ID HO747604; SV 1; linear; mRNA; EST; FUN; 711 BP. XX AC HO747604; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050043.T3_H11_081 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-711 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 0e7c8213b1f9d4df741f5a7af9b8af6a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..711 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 711 BP; 130 A; 250 C; 196 G; 135 T; 0 other; gggccttgaa tcccgcctct acccccttct tccccggagc aatgcggctc aacgacgatg 60 aaggtgtcgg gcacgcgcac gggaacgggc ttgggaacgg gaacatccct ctcggcttca 120 ggcagtctgt cgtgcgggaa caccagtaca gcacatcctc gtcgctctcg atctcccctt 180 cggactaccg ctccgtcagg tcttctccga gcccgccaca ggacgagaga gaccggcgga 240 tcgatggtgg cccgcgagca ccgtcgtccg cggaattctc gcgcaactcc cctggcttcc 300 gccagagcca ggtggaccag tccgacaagg cctcttactc gcatctcctg agcgcgtcga 360 tctcccgcga cctcaacatc tcccccaaga gccagggtcc cttggacggt gatgagaccc 420 cgggtcccac gtcagggccg atccccatga acgggcagat ggccgccacc gcgtccttat 480 acaacacaat ggcagtaaga agcagggaga ggtttgggac gccacccgtt atgcagcagg 540 agcccagcag cgtcaggtca ggagggttca tccatccccc gttcgtctct tcctccccct 600 cgtcatcgct cgactccggg agccatttcg ggcccaactt cgagttctcg gccacgtcga 660 acttggaggc acagctccga gcttctcctt tgttcaacga tttgttggat c 711 // ID HO747605; SV 1; linear; mRNA; EST; FUN; 721 BP. XX AC HO747605; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050043.T3_H12_082 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-721 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; f78562d82efca906c287a746a4a9f5d4. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..721 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 721 BP; 136 A; 259 C; 151 G; 175 T; 0 other; acctctcccc ctttccgtca cagaggtttt ctctccgctt cctctgtcct ctgcctgacg 60 accttttccg caccttctca tcgctcgtcc gcccccaaga caccaccaag tcctccccgc 120 acgaacaagc cacaatgtcc aagcacttct gctgctgtat ccctgtccgc gcagcggtct 180 tcttcttctc ccttctgtca ttccttgcct caggattgac cgcagccatt ggctggtacc 240 tcgtcttcct cattaactcg gacaagcttg aggaggccga gaagaacctg aatgaccagg 300 ataagaaaac actagatgcc gttgcacaca agtacaagtg ggccttcatc gtcgctgcgg 360 gcgtcttcac cctcattgct ctcatgtcct tcttcggttt cgttggttct atcattcgca 420 accgccgcat ggtgaaggcg tactccttca tgaccattat catcttcatc ctcggcacgg 480 tcgcgactgg cttctcgctc tacgcgacct tcagccacaa gccgctctgc gtgacgattg 540 ataacgtcca gtcatgcgcg acctccaacc tcaccacagg ccagaagatc ggctacaccg 600 ccttcgccgt tgtccaatgg ctaattgatc tctacatcgt cgtgatcatc cgccggtacg 660 ccgagcagct cgacgaggag cgcgagtacc gccacgagtt ccgcctcaac cccaccgcgc 720 c 721 // ID HO747606; SV 1; linear; mRNA; EST; FUN; 650 BP. XX AC HO747606; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_A01_015 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-650 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 14bd0e0431bd491ebbbd6f8c240351c2. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..650 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 650 BP; 113 A; 259 C; 150 G; 128 T; 0 other; gaggatggat acgcgccggt cgcagaagag gacgcagttt agggcgggct ttttcgcgtc 60 gcctggcgcg gagggcctcc ctgcagtggg cgcctggcta ctggggtccg catcagactc 120 gtcgcttccg cctcctttca ccttgaccga agaccaggct ggcctccacc ttcacatatc 180 ctactccctc gaccttcctc cctctcccat cccttctcgc cctctctccc ctcccccgcg 240 tgcacccatg cccgcgcgac actactccaa gttcatcccc ccgagcctcg ccatgagcga 300 gctcgtatgc caaaagctcg acaacgacgc tacggacatg ctcatcgaca aatggctcga 360 gcggccccac cgcccagact cataccacga ctggttccag cgcgtccgcg agcgcaagca 420 cctggccaat gccaacgccc acatcgcccc ccgcgaccag gtcctcctct ggaacaaggg 480 cgtcttcgtc cccgagaaca tctttacccg caccgtcgat accggccgcc tcattcccct 540 cgaccgcacc tgggtcaccc atgtctacca tcaccgctct atgaagcggc ttactatgat 600 gcctgtcatt ttcagtatgc ttccggaact catgctcctc cgcggcatgc 650 // ID HO747607; SV 1; linear; mRNA; EST; FUN; 645 BP. XX AC HO747607; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_A04_032 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-645 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 6b0fe5512aed57bd21760c578e6a1b83. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..645 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 645 BP; 135 A; 187 C; 175 G; 148 T; 0 other; cagccttcta tcatcagctt gcggaggccg aggatggccc acaatcactc tcatggcgga 60 ggctgccacg acgaaagcga ccatcatgac cacgaccatg gcggcacgag ggctttcttt 120 atcttgtcct ctgcttgtgt tggtctgtag gtcggcccga acgctactcg ggctcatgtc 180 gtctccaaaa ttctccgtct tcttgacaca gctggcggcg tcgttgcgcg aggagaaggg 240 tcctgaactc gcctacttgc tgaaaccgac gagcgaacac gggaaggcct tggtcaagga 300 cttccgggga aacattacca ggcaatcact gtcacactac gaaggcagcc ttgagagccc 360 ttgggacgag gtcgcgatcc agtatgtcct ggtcgtgaac ctttgtggca aaaagcgggc 420 gacggaggca ttcaaggagc agtgcaccct actttcgtga gtgagttgcc aatccgccgt 480 ctgcagatcc ttcacttaga gcatatacgt ctgttccacc tcaggctctt cttgcggttc 540 ttcgccacca acagtggttg gaccctcccc gccctgttct ccattctgag agatttgcga 600 gacctcgcgt ttgatgcaga cctggaagcc atgtcaaatg gacaa 645 // ID HO747608; SV 1; linear; mRNA; EST; FUN; 595 BP. XX AC HO747608; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_A05_047 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-595 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 02931d4a674c3cb8c5aa0defbc091e54. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..595 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 595 BP; 149 A; 160 C; 176 G; 110 T; 0 other; gcctgcacag aatcccatct ctttgcggct ctacaaggta ctcagtgcga acttcgacga 60 tgacgcgaca cgcgaggccc tagacaccct tgccgagctt tatgtgccct ccagttctac 120 cgctggaccg ggacatggtg cgaatgggaa ggggaaggaa gtcagactcg atggagacca 180 cgatggagac gcggaggatg atgtggaagc cattcctgga gtaaagctga aaggactcgt 240 cggggcggta gcggtggacg aaggcacgcc aggagacatc gcagcaagag cacggaagaa 300 tctccgaagg gatgtcgaga gcaagcttgc agagtcgagt cgacagttct tgactgcttt 360 cggagaggtg gacaagcaac tagatatact gcaggagcat atcggcctga tgcgcacacg 420 gtgtgatgag acgcagactc agctgcacat gaccaacaaa gcttgcaagt ccctactcga 480 ccgggcaggc tcgcttcgag aagagaggca aggtgttgcc gtccgccaat ccattatcac 540 ccttttcctc tcccggttta ccctctctac gagcgaaaag gacgccatca cctct 595 // ID HO747609; SV 1; linear; mRNA; EST; FUN; 759 BP. XX AC HO747609; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_A06_048 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-759 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 6496242cca71b86909a8fa22d0d4a9da. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..759 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 759 BP; 226 A; 153 C; 161 G; 219 T; 0 other; tgcataacat tgctttttaa acaactggca gggccttcgg aataaggaag ttataattga 60 ggacacttaa atataagtga ggcagtgatc caggaagcct gtattttaaa agacaaaggg 120 acattcatga attggagagc attgcttttc agggctccag gactcagaaa cttgaaagca 180 atctgaaatt ccagtagagg tgagttccaa tttgattttt ctgggattaa ttcattaaaa 240 gctaaaaact gagccctcac tcataacctg atatgtaatt tcgaggagta tagatgaccg 300 accctggagg ctctgttctg ttgttttacg caaatcttca ttttctagac caagccccac 360 tctggtgttc gggatctgct tgcagacact cgtgatttgt agggtcatcc aaagcagttt 420 tctcacctgc agtcagtttt catcctcagg ggacatttgg taatgcatag aaacattttt 480 ggttgtcaca atgcgggggt gctattggta tctagtgttt agatatgttt ccaaacgtct 540 cataaagcac aggacagctc cacacaatga agaataatct ggcttcgaat gataatactg 600 tgatcaaaat caatgatttc caaagagtag tccccaaact agccacttca gtactggaaa 660 catgttagaa atgcaaattc tttgcctcca tgtccatcca acagcatcag aaacttgagg 720 tggagcccct gcaatctggg tataacaagt tctccaggt 759 // ID HO747610; SV 1; linear; mRNA; EST; FUN; 754 BP. XX AC HO747610; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_A07_063 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-754 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; c9047d92aea73a81b40008c62d8ea5cf. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..754 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 754 BP; 137 A; 237 C; 196 G; 184 T; 0 other; ctgccttccg tccccccgac ttcgaaactt tctccatgtt cacctcccgt ccctgggcac 60 ctcttgccgc tggcgcattg ctggcgtcga gagcgtatgc acacgataat ggcatggaca 120 tgagcatgga cggggcgatg agccttgcat ccggcaacat gattccgtat cttcatttca 180 cccccggaga catcctttgg ttctacggct gggtaccgtc gagcaagggc gcgatggtgg 240 gcacttgcat cgggttattc ctgttcgccc tggtcgatag atggcttgcg gctgcccggt 300 cggtgatgca ggcgcactgg tccaaacggg cacagattgt tcaggctgac aggctcaacc 360 tccgcgagaa gtctcccggc acctcctggc ccggtcgcat ccgccatgtg gctactctgc 420 gcacagggct gcccttcatc ccagcccacg acatctcccg tggaatcatt catggcgcgc 480 agtcgctcat ccacttcgca ctcatgtttg tcgtcatgac cttccaggca tccttcatca 540 tatccgtcgt ggttggtttg ggcgttggag agacgctgtt cgggaggttc accgctcacg 600 ctgggcacca ctgacctcat gctcctcctt ctccgtaccc ttacgccttc gctgaactgt 660 gtcggacggc aaggactctg tataacagag atgtactatt acttgtgtat agactttggc 720 cagaccaacg accatgtcgt tctcaaaaaa aaaa 754 // ID HO747611; SV 1; linear; mRNA; EST; FUN; 606 BP. XX AC HO747611; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_A08_064 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-606 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; c78a657b5d071b46d0f51f989a17320d. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..606 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 606 BP; 145 A; 169 C; 164 G; 128 T; 0 other; agaattcggc acgaggatct ccaacatcga ggtcgtctac ggcgacgagc cctcccaagc 60 tccctctggc cgcgtcctcg agaccaacca ccagaacgac gacatcaact accagtttgg 120 cggaaaatac gtctggctcg tccctgtctt cacgaccgac cgcgacgccg cggtcactgg 180 tttcgacatc gccatccaag ggagcgccga cggctcgctg agcgacctcg ccaagggtgc 240 gggtggcgac taccgctacc tccttctcga gaagagcgcc acgcagacgg agagggttac 300 tgaggttgct ctgctgcgtc ttgacaatgc gatgaccgag cgtcctgcgg ggtgggctgg 360 aaacactatc gacatcaaca aaggccgcgg caagagctat ttgtacctcc tgtggaagac 420 cgcttgaagc tctcgttctt gttaaccatt gaacagctat caaacacttc ccgtagaact 480 gtggcttgcc agtagccagg atggcgatgt atctgagtgt cccgaatgtc gtagagttgt 540 tgttgctttg tcgttgtgaa tgtcaagtgc gttagcgttg tccaataaaa aaaaaaaaaa 600 aaaaaa 606 // ID HO747612; SV 1; linear; mRNA; EST; FUN; 759 BP. XX AC HO747612; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_A10_080 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-759 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 12cb9587a43701387aa7a585df2ae28b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..759 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 759 BP; 150 A; 228 C; 192 G; 189 T; 0 other; ttctatccgt acttcccgac cggtgtagat gctgtgcgtt gctactgccc tcgatcgtgc 60 aaatactcat attgacctcc tcactacagc gtgcagcgtt cgctctcttt gacaaggatg 120 gcaacggtga tatctcaaaa aaagagatgc gtgaagcggt gcggcgaata tatcgcgagc 180 ggaaggccct gacggcgagc ttgaaggatg ttggttctgc agtggctaag ctcgatgcgg 240 tcatgctctc cgttgttgca ttaattttcg tcttcatctg cctgcttatc ttcaaccgca 300 agaacaccct ggcatccctc gtcccgctcg cgaccattat tgtcggtttc tccttcatct 360 ttgggcattc cgcacagaca ttgtttgaat cgctcatctt catcttttct acgcacgtct 420 ttgatgtcgg agaccttgtg atgattgacg aacagcccct gttcgtccgc gagtttggcc 480 tgttctcgac caccttccga cgtgtcgatg gtatggagat catcgcaccc aactcgctgc 540 tcgcctcctc gaagctcgtc cacaacctcc gccgcagcaa ctcgatgtgg gagtccacga 600 cggtcaccgt ggcgtacgac accccccttg aggtgatcga gcaactccgc acgcgcctgc 660 aggcatacgt gaacgcgaac aaccgcgagt ggtccggcgt cggcgtgaac atcgacaaga 720 tggagcacca gaacgcgatc tcgctcatcg tggccatgg 759 // ID HO747613; SV 1; linear; mRNA; EST; FUN; 550 BP. XX AC HO747613; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_A12_096 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-550 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 43ef2f2d4e3bc1833d9bbda55632cb24. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..550 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 550 BP; 121 A; 164 C; 135 G; 130 T; 0 other; agaattcggc acgagggcgg tcccgtcaat gcaccgctcg atgacggtgt gatccacttt 60 ccatggagag ttggcgcact ttgccgcatc actagcagtt tccatctcgt ttctcgtatc 120 agcttcgttt catcatccaa cgcggatgac accgcggctc tcccgctcgt gcccgatcca 180 attacgtcca tccccgcatc cttgacgatg cgggtttaca acgaaccgac gccacaacag 240 ctcaagttga tcctgttgtg tagcgcatag catctcccct cgcatgacac gaaccagtgt 300 tcgtgtaacg cttgcgtccg gctccattgc cgaacaggct gcagcccggc gtgaagcttc 360 cgaaaatcaa gtaccccagg acatccccgc cgtcgcggct tgcgctttgc ccacaacggg 420 ccggcgcggt tgcggcgctg cttcaccgtc aagctcgttg gagccgtgga gacatatatt 480 ttgtagacgt atctgttgac gttgtgattg aatgtcattg gtttcatgtc aaaaaaaaaa 540 aaaaaaaaaa 550 // ID HO747614; SV 1; linear; mRNA; EST; FUN; 636 BP. XX AC HO747614; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_B01_013 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-636 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 718935c275dcc9eb9055f2e7233d14c9. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..636 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 636 BP; 152 A; 196 C; 168 G; 120 T; 0 other; accgttgccg tccccgacca agacggaggg gcagatgccg cgtccaagaa gaataggaag 60 cggccccttt cgtgcggtga atgtaggagg ttgaagctca agtgcgatcg cgtgtttcct 120 tgccagtctt gttgcaagag aggatgtgca gaaatatgcc cggacggcgc attgaccggt 180 ggcaagggga gcaggttcat cttggcgaat acggaacagc tccacgataa aatcaagtcc 240 atgagcctcc gcatacgcga gcttgaggag gccctccaag atctccagtc gggccaccca 300 ctgctccgcg aggacctcct gctcatcaag aagtcagcag accttttcgg cgtggattcc 360 aaccaggctc agtcgttagg aggggacaga cgcaacgacg cacagcacgg attagtcaac 420 ccactttccc ctcccatgct aagcgaccgg cagagccatt cacctcctgt caatggccat 480 cgagatgact acggcatccc gccagacatt ctcggtgtca gcaataaatt cccagccccg 540 agtactctta gcgtcgacct aaatccaact ctacgcaagc gcatacttga cctgcttcct 600 cctcagaacg aggcccggta cctctgcgag caagcc 636 // ID HO747615; SV 1; linear; mRNA; EST; FUN; 514 BP. XX AC HO747615; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_B06_046 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-514 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 9fa5e5d1f3cb316f452b8b8b3c49995e. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..514 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 514 BP; 109 A; 147 C; 109 G; 149 T; 0 other; ggcaccgttt ccttcttctt tgttgccttc gcactcaagt cgcggcttgc catttttctt 60 ttgccacttc tcccgcgcta tttgttgcct ttagcgtctt agttttaatc ccctcctcct 120 caacccatcc ccacccgaca tcacttaaca atggatccgc aacaggcgaa cgctggccag 180 cagtaccagg ctcacctcat gaacatgtgt gccgctggca accacgaccc cagcaagaag 240 tatggcatct gcggcattct caccgctatt ttctgcttcc cctgcggtct catctgtctt 300 ttcaccgacg tcgagaagcg ttgtgtacgg tgtggcgcca agtgttaaac gggctccgga 360 gtgacagtgg ttatcgtcga cggcggtcga cacggtgcct gttgagatat catatgtgtt 420 gtctgttcga taatcgactc tatcgttgta tatcctgcac aacgtgcctt cgttaattcg 480 tattttatat gtcgacaaaa aaaaaaaaaa aaaa 514 // ID HO747616; SV 1; linear; mRNA; EST; FUN; 635 BP. XX AC HO747616; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_B07_061 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-635 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; b88611363b50a64bb40211632227c58b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..635 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 635 BP; 167 A; 170 C; 174 G; 124 T; 0 other; gcctctcggc gtaccaggag ctgaagctgg gcaagaaggc caaatacatc atcttcacgc 60 tcagcaaaga cctcacggag atcatcgtcg agaagaaggc ggagccgagt gccacctacg 120 acgatttcgt cgcggacctg cccgaggctg aatgccggtg ggccgtgtac gactttgact 180 ttgagaagga cgacggcggg aagcggtcca aaatcacttt ctactcttgg tcacccgacg 240 actccaaggt gaagcagaag atgttgttcg cgtcgtcgaa ggaggccatc cgacgctccc 300 tggtcggcat cgcggccgag atccagggaa cggacttcag cgaggtcgca tatgagagtg 360 tgctcgacaa ggtctcgcga ggtaactgag cggttgatgc ttacgacgac cccccacaca 420 cgcgcatgac gacatcgatg ccgatgtcca ggactcccca ccccccttgt ttattgaaga 480 ctcggagatg gtccgaatga tgatgctggt ggaggcttgt agtcatataa cgaacgatct 540 ctctgttggt cccgatgccc acgagctcca gaagtgccgt agaactgtat aaatcccata 600 cttttcactt gaaaaaaaaa aaaaaaaaaa aaaaa 635 // ID HO747617; SV 1; linear; mRNA; EST; FUN; 765 BP. XX AC HO747617; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_B08_062 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-765 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 047107e6eeb303c3e1a2ced0df5e66c8. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..765 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 765 BP; 146 A; 245 C; 205 G; 169 T; 0 other; acagtcacct tcagctacac gacgccattc tggaacttta agcaatggga acttgaactg 60 gactggctcg cgctccgagg cgtcaatctc ccgctcgcct ggaacggcta cgagtacatc 120 ctctccgaga cattccgcga ggttggattg accgacgccg acatcgcgtc cttcttctct 180 ggccccgcgt ttcaggcgtg gaaccgcttt gggaacatcc aaggccactg gggcggcgct 240 cttccggctg cgtggatcaa cgaccagttc gagctccaga aacagatcgt gaagcgcatg 300 ggggagcttg gtatgacccc cgtcctgccc tcgttcaccg gcttcgttcc ccgtgccctg 360 tcatcgctct atccgaacgc ttctattgtc aacggaagtc aatgggcagg cttccctgcc 420 gctctcacca atgtcacttt cttggagccc tttgacccgc tgttcgccac gatgcagaag 480 ttcttcatct cgaggcagaa cgatgcatat ggtaacgcat cgcacatata cacgctcgac 540 cagtacaacg agatcgagcc gttcagcggt gccaccgcat accttgccaa catcagcgcg 600 ggcactttcg caagcctgcg cgccgcggat cctgatgctg tctggcttat gcagggctgg 660 ctgttcctcg ccgatgccgc gttctggaca aacgagcgcg ttgaggccta tctcggcggc 720 gtacctggga atgacagcat gatcatcctg gacctgtact cggag 765 // ID HO747618; SV 1; linear; mRNA; EST; FUN; 685 BP. XX AC HO747618; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_B09_077 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-685 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; f40c57c9e5dd39ad7ec52cd0d2f6bfea. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..685 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 685 BP; 101 A; 208 C; 192 G; 184 T; 0 other; cgcaggtgcc cgactacgat atggcgagcc gcgggttctt gggcgggggc atcattccac 60 tgtcgagctt gcgcgacttg ccgacgtacg agagcgtcgc tggcggaagg gggtcgctgg 120 gcgcggggga gaggagcttc agcgatgggg acctcgcggg catgttcgcg gcgcatcggc 180 tggggccgag ggcggggcgg tcggcgctgg gacgggtgcc gccttcgaat gggggtgcgc 240 ctgctactgt cgctacgcct tgagccttgg tccggatgct tgcgttccgt gtcctctgga 300 gtaaaggtct ccgcctcgct gtggacttat cattattgtt ctgggctcct ctccgtctgt 360 ccggtcttcc tctggtcttc aatcctgctt cacggtcgca tccaccttcc atcatgccgt 420 tttcctcctg gaccctcctc gacgccgtct ccgcctcttt cgactacatg cggactattt 480 atggctttaa aagacatatt gtacctcaca tttagactgc ctcgctcacg cattgctaac 540 gcctctcgat ttacgaaaag tacatatcca tcccacgcat actagacact gttggaactc 600 cctttaggct tccccgtata gactcttgtc ctcattcgtt ctgttgttgt ccatcacagc 660 gtccttctcg catttgtatg tactc 685 // ID HO747619; SV 1; linear; mRNA; EST; FUN; 624 BP. XX AC HO747619; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_B11_093 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-624 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 15f54f4f3fe54f73854787fae44f24b9. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..624 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 624 BP; 113 A; 243 C; 123 G; 145 T; 0 other; ctttgctctc ctgccactct ctcctagtcc gtctgtcagt gtcacgcccc ccgtcttgtc 60 cacgttatac tcgccctacg tataccctcg ccctctaatt atctgtctct gctctcacgc 120 tcttttgcct cccacctcgc cctcttcacc gacctcgccg cggacatccc tcactcggca 180 cactcctctg cactgcacgc agaggtgccc gcgcacacga ccgccaattg atggaagaca 240 agagagccca aggagctggt atccgccggt tctacgattc acaatccacc accaccttcg 300 accccaatca tgacctcgtc acctcccctg tcttccaccc ggccgtcctc gcgggcctcc 360 gcctcacctt cgcgacttac gcgctgttcg tcgcactcta ccagctgatc gaggaagcgg 420 tgaaggaggg cgacgcgcga acgttcttct cgtactttac ccacctttcg tatattggct 480 tgatcgcgta catgtacgct gcaggcgtcc aaacactctg ctatgtattg aaccggagac 540 aaggataccc tctccagcga tggccccgcc ccctccaaca ccttcacaag ctactgtact 600 ctaccgtgac cacctatccc atgg 624 // ID HO747620; SV 1; linear; mRNA; EST; FUN; 785 BP. XX AC HO747620; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_B12_094 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-785 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 15172b2643783f4b1850ba572dd4d8e3. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..785 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 785 BP; 183 A; 241 C; 199 G; 162 T; 0 other; atcctcactt ttcgccgccg acatcaaaat gcagcgaggt ctgcccatgc aacctacggt 60 cgtcctcctc aaggagggca ccgatacctc ccaaggaaag ccgcaactcc tctcgaatat 120 atctgcgtgt cttgcgatcg cggagacact ctccagcacc ctcgggcctc gcggaatgga 180 caagcttatt gtgaacgagc gtggagaggc tcagatcacg aacgatggcg cgactatcct 240 caagctcctg gacatcgtcc atcccgcagc gcgtacgctc gtcgacatcg cccgggcaca 300 agatgcagag gtcggtgatg gaaccaccag tgtcgtgctc ttggctgcgc gactgctgaa 360 ggaagtccga gggttcatcg aggagggtgt cagcccgcac atcatcatga agggtttcag 420 gaaagcgtcg gagcttgctg tcgaacacat caagtccgtg cagattacag tagacaagtc 480 aaatgctgaa cagttccgct ccctcctcct caaatgcgcg tctacatcca tgtcgtctaa 540 actcatccac tcagaaaagc cattcttctc caatatggtc gtggacgccg ttcaatctct 600 tgaccaggac gatctcaacg agaacctcat cggagtcaag aaggtccctg gtggtggcat 660 gcaggactcc ctcctcgtcc gcggtgtcgc tttcaaaaag acgttcacct acgctggtgc 720 cgagcagcaa ccaaaatctt tccgcaaccc gctcatcgtc agcctgaacg tcgagctgga 780 gctga 785 // ID HO747621; SV 1; linear; mRNA; EST; FUN; 640 BP. XX AC HO747621; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_C01_011 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-640 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; f4da44d2e235363d49d3a4b1da3ee4d1. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..640 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 640 BP; 125 A; 202 C; 172 G; 141 T; 0 other; ctcaacttct ccctcgctca cggtccgccc atagagatca gtgggtttca cagtggatac 60 gtgccagccg gatggctcgt atgtgaggca tgcatgacga cgtcgctggt tcaggagata 120 gtcaagaatc agctcgtggc caaccttcgc cactccggcc gtcgtgtgct tgcacgcggc 180 taatccgaac ggtgtgaaga ttgcgaaacc ttcgcgtctt cgtccattcc gaattgtctg 240 tttggacata tacccagcgg gcctccacca gctgcgcctt gcttctttct caagcacctg 300 tgcatacaca cgttccttcc tgtcaagctc ccagtactta taccttacaa aacatgctcg 360 ctctcaccgc ccgatcgcac gcgctggtag ggcgcgcctc tgcagtcgcc cgctgggcga 420 gcaccgcggc aagcaaggat cagttcaaga ttctcgtggt cggaggaggc tctgccggtc 480 tgtcggtcgc gaaccagctt tacaaccgct ttaagcgcgc cggaaaggcg ttgaacgcgg 540 gcgacatcgc cattctcgat gccgccgaat atcattacta ccagccggcg tggtgcgtag 600 acgttcctcc atgtgcaccg cgatcagagg agcgcaagac 640 // ID HO747622; SV 1; linear; mRNA; EST; FUN; 514 BP. XX AC HO747622; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_C03_027 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-514 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 94ea2d04f23fa97f7f231ae64ba34f09. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..514 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 514 BP; 118 A; 188 C; 111 G; 97 T; 0 other; cacagatggt gtcctaaagg cgggagatat agccaactca cagacgcaga atgtagcaga 60 catttgaagc gagtcccggt accgtacccg gtcaactgga agaagcggga aagatttctc 120 tcgaggaagc agaatgtcat gctcatcagc gcatttcgtc cgtcctctcc gaagacccga 180 tgttcgacaa gtctcgcagg ccgtttctct ctcgcacaca gctctatatc cgtggattag 240 ccccgtccaa ctccacatcg caggtacatc ccattcagtc ccgctctccc aaccccgccc 300 caatcgcgac acatagcatt cgagcccgtg cttaaatttc aaagctcccc caagctccac 360 gccaggcacg gcaccctcat cgctcaccac gccattcccg gctggtacat ccagaccgag 420 ttcgcccacg acgtcaacgt cactgcgctg gggaccactg ctacctacct ccccaccacc 480 cgcaagtttg agctccgcac gcccgccccg actg 514 // ID HO747623; SV 1; linear; mRNA; EST; FUN; 638 BP. XX AC HO747623; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_C04_028 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-638 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; a50d911e19637f1c5814f610db2b74d2. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..638 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 638 BP; 144 A; 186 C; 195 G; 113 T; 0 other; gattcgtctt tgcatcccct gctcttacgt ttcattttcg ccgcgtcctc tgtcaatgcc 60 cacgccaccg ccgttcacga accccgatga tttcgccccg gtcaagggcc ggcgtggcca 120 atacttctgc cagatatgca ctcctgccgg ctacaagcag ggacaggcca tgagtctcaa 180 ggccgccttg cgtcacgaaa gggaaaacgc caaacacata gagaaggtcc aagagaagat 240 taggaacgac tggaattacg accctccgtg tgactggggc ccgattcgac tgccggaggg 300 ctctacggct tgggagcagt cgtatcccgc agaccgggtc gaagatttca ttaccttctg 360 gctcgccaac gtcgccgctg aagagcgtgg ggagaaggtg caaaccatgg actcattcat 420 cgacaactac aacaagaagt accaggactg gctcgatagc tggtatcacg gcgaggccca 480 ggcttacgtt cagggccatg gagacgtgga agaggacgag gaggagggag gcggggagga 540 ggatgggtgg aatggcgttc ccggcatatg ggccggcgac aactggtccg cccagcccaa 600 ggagcagtgg cagccctgcg ctgccgaccc cacatcgc 638 // ID HO747624; SV 1; linear; mRNA; EST; FUN; 684 BP. XX AC HO747624; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_C07_059 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-684 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 81d381887eaba2286cbf40058a3628d1. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 684 BP; 125 A; 239 C; 154 G; 166 T; 0 other; cagacgtcat ctgctccgac tcgacctcga cctcgccaga cgtcccctct gccctcgctg 60 cgcgtccttg ctcggtcttt gtacgcccta acgacctgac acgacccctg cctgccaggt 120 ctctgaaccc cccactggcc cgcgatacct gctactccca accgtccagc ctttaggatg 180 acgcaactat tcagagacat gcgcctctat ggcaatatca tcctgctgct cctcggcgct 240 accgtcttgg gtataacagc atactgggcg agtatcttcc tgcctaacat ccgccacgat 300 tactccatct ttgcgctcat tgttccggcc ttgaccatcc tgctactact agttggcctg 360 cagtggtcaa ctccgagaac agaggctttc ttcctgttca tcttcggggt gttatggctc 420 gccatggcgt cttggacgac cgacatcatc gggaacactc aatgcgacgc gttgtctagc 480 cagactgtgc cgaccaagag cggaacgctc cctgcacgaa gctggtgcta cgaaatgcgt 540 gtagtccaag ccttttcttg gatgatcttt tgcctctacg caatcttcct gtatatcctg 600 atctcactca ccacccgcgc caaaacgctg ggccgtccct acgcctgggc ggaacccatc 660 atcgagctcc cctggttcgg ccag 684 // ID HO747625; SV 1; linear; mRNA; EST; FUN; 634 BP. XX AC HO747625; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_D05_041 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-634 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 7e2f20a1cd63f27b005e8564c96c2691. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..634 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 634 BP; 128 A; 233 C; 151 G; 122 T; 0 other; tctcaccagc tgcatctcgc gccctcagtc tcaccacttg cctaccgctc catcctccat 60 cgctggcggc caccgcgtct gattcagctg ttccgcacct tcccagccct cgtcttctag 120 cttccatctg ccgatcgcaa gatgttcaaa aagttccaac cgtcctcgga cgtctcgggc 180 caagttagcg tgaagtcgtc ggtgcagagg tccatccgat ccaatatcct cgcgcaatgg 240 aagatcaacc ccgaaacatt cgagcagata tggcccaaaa aggagcccct cacgctcgta 300 aagtgcagag agcacatctc catctacacg ctgcatggag agccgctgtt cttccagcac 360 ttcgacggcc cgttcatccc cacgcttcga ctccttcaca aatacccctt cattctcccc 420 accgtcaagg tggaccgcgg ggcgatccgt ttcctcctcg ccggcgccca catgatgtgc 480 cctggattca cgtctgcggg cggttccctg cccccagctg agcaggccct ccccgccagc 540 acgcccgtcg ccatccacgc agagggcaag gagcacgcgg caggcatcgg catcaccaag 600 ctgggcacag aggagatcaa gtcggtgaac aagg 634 // ID HO747626; SV 1; linear; mRNA; EST; FUN; 666 BP. XX AC HO747626; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_D07_057 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-666 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 4a6eb1b45bbe256bab2983b94be25a90. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..666 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 666 BP; 154 A; 192 C; 194 G; 126 T; 0 other; tgcggccacc acctctccct tactacatcg cttcgcggcc gccgcgtgca cacctgggaa 60 cttgctgcga gtggagggca aggcgtagga gatgaaggcc ataagaggtg tgcttctcac 120 ctgcgactca gccgtcaagc agatattgtt gacgatgaac gagaagcaaa gcttcatcat 180 tgaggatcta gatgaccacc acgttgtcat caaggcggac gaagagtatc gagtgaggag 240 ggagcttgag gctgagctcg agaagaacac ctacagcttg gaatgaaggc aagcagtatg 300 ttctcatcgc agactggtcc tgtatgcgcc aatcctgtac gtacccgcaa ctggcttcaa 360 tagcgatgtc gagccctccg gaagggccca aatcggattg tgtgcaggcc gtacatgagc 420 acgttccgga tgaaagaccg ctgcggaaac ggggttcacg gacattcaat gatacattag 480 gctgggacgg atggaaggag cggccagacg aaccttcacg acggcttcgg tcttgaccaa 540 gtgcgcacgg ctgccgacga cgaccgccgc ctcgcccttg ggcgagatct cgtccatcgc 600 cttccacaac gcctccaggt cctcgaaccg actcgcgccg tctgcgtcga ccatcagcag 660 ccgctt 666 // ID HO747627; SV 1; linear; mRNA; EST; FUN; 711 BP. XX AC HO747627; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_D08_058 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-711 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 7a29abf39ed2f85e94e2d77820c87ef3. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..711 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 711 BP; 155 A; 261 C; 167 G; 128 T; 0 other; catttctccc ggtcgcctct cccgagtctg tcgagtcacc gcgtacttaa tctcagctaa 60 accgtctacg atgatcctca acaaccccga cgcaccacag gagatgaagg gcaacgtacc 120 tcctgtcccc gaaaagccac agcaggctgc tgaggctctc cctccgcctc cgccttactc 180 gccgcctcca agcaatgttg cacgacagat gccaataccg ggacctgcgg tctcgggacc 240 atctgctgtc ttccaaccgg tgaacccgca gagggttaac tactttgaac tcttctcaaa 300 acacgacgct atttcaggga catacctcat cgaccctgag ctgccctcac cgatggcagg 360 tttgtctaag gccctccgca agaaacctga ctcggtctgg ggcaagccca agggctgcaa 420 gaagtcgaag aactacaagg agctcaatgc gtccttccag acacgccacg gacacatcac 480 cctcgacctt gccgtcccat ccaggaacct cgggaacctc agccctttcc ccggagacaa 540 gatgcgcacg cgcatgtacg tcgccacgcg acacggacgc atcaagctcg acgtgcacga 600 ggtgaagcct agctgctcgc ttgacctaca cgtcgagtcg cggcacgggc gcatcagcat 660 cctcctcccc ccgaccttcg acggccccgc tcgtcatcca cgcgcgctcg c 711 // ID HO747628; SV 1; linear; mRNA; EST; FUN; 567 BP. XX AC HO747628; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_D09_073 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-567 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 4c68d4c7ff3dfccc60c73d08376b3563. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..567 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 567 BP; 138 A; 186 C; 138 G; 105 T; 0 other; ctgcaacgaa gaccaccaaa aagggaggca agacgcgctc cgccctccaa gatgtcgtga 60 cccgcgaata caccatccac ctccacaagc gcgtccacgg ccgctccttc aagaagcgcg 120 ctccctgggc cgtcaagtcc gtcgtcgact tcgcccagaa ggcgatgggc acctccgacg 180 tccgtatcga ccctaaactc aaccaggctc tctgggcgca gggcgtcaag tccgtccctc 240 accggatacg cgtcaagctt gagcgcaagc gcaatgacga ggagaacgcg aaggagaagc 300 tcttcaccta cgctagctac gtccctgtag actccttcaa gggcctcgag actgttgtcg 360 tcgacacgga gtaaaaattt cccttgcccc tctgccatcg cttctatgtc acctcctgct 420 cgagttgctg tggcctcggg tcgcaggacg gatgggaggt tcgtccactg tattatgcct 480 tgtcatgcac cgcacatgcg cccagccgca cacgttacat actgcctata tgctatgtga 540 agcaacccaa aaaaaaaaaa aaaaaaa 567 // ID HO747629; SV 1; linear; mRNA; EST; FUN; 666 BP. XX AC HO747629; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_D12_090 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-666 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 9e8e80cda0599b82020adeb399eaef3b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..666 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 666 BP; 108 A; 282 C; 146 G; 130 T; 0 other; gacactttac acctcgtggc cctctcgccc ttcccctcct ccccctcgtc tcgcagcctc 60 gtcgcttttt agcgcaccct ccactctttt gcacagtccg ctccttcagc cccctcacac 120 acccgaaatg ttctccgccg ccctcctctt cctcgccgcc gcggtctccc ccgccctcgc 180 gaccgtctac gtcacctcgc cggtggccac gacgtcatgg gccgccggcc agcagcagac 240 cgtctcctgg aaggacgacg gcaccgcgcc gtcgctcgcc aacttcggcc ccagcaaggt 300 ctcggtctac gtcggttcgc agacccaaca gactatggtt cagcctatcg tcgcctcggt 360 cgatctctcc acaaccagct cgatcgtctt cacgcccgac gccagcaccg gtgagaacgg 420 ccaatactac ttcatccgct tcgagtccct cagcttcaag gaccctaaca accccgcgta 480 ccctgctctc gcgttctcct ccaagtacac gatgactggc atgactggca ccttctccgc 540 cgacgttaag tcccagattt ccgctgccgc taccgccacc gccgggaatg gcgcagctac 600 ttctgccgcc tccgggtccc aatcgacgcc tgctgccacg accagcaaga tgaccagcgc 660 caaggc 666 // ID HO747630; SV 1; linear; mRNA; EST; FUN; 540 BP. XX AC HO747630; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_E01_007 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-540 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 18763b15fb75172f11bab1769a55216e. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..540 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 540 BP; 122 A; 181 C; 140 G; 97 T; 0 other; gctcttcgac tcccgtttgc aacaatctca ccatgtctga caaacctgtc acctccttcg 60 acgagttctc caagctcccg cccccgctct accccgccgc agctaagata atgaagctct 120 ctaacgccta cgtcgtcttc aacgacgccc agaaggtcac aggcttcaac tggagcggct 180 tcaaggacgc cgtcgtcgcc cacccgggca cagacctcgc gttcgacaag ctgcaagcaa 240 cgtcgatcac gaaccagacc gcgtccgtcg acgacatggc aggcaagatc gcgcagttcc 300 tcgtcgacac gtttgcgctc aaggcagcaa accaggacgg actaaaggcg cagatcaact 360 cggcgttcaa caacctggac aagaaggagc acagtgggtg gttcagctcg tacacaagcg 420 agggccgcgg gtcgtcgtgg gagtaccgga tcgtgttcgc gctgccaaac ccggaccacg 480 ccgactggtt ctactcggtg gtgtcgacca tcaagcttac tgcggatatt ctcgagaccc 540 // ID HO747631; SV 1; linear; mRNA; EST; FUN; 590 BP. XX AC HO747631; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_E02_008 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-590 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 81adc362749813ecd57f1a1a27072651. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..590 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 590 BP; 159 A; 163 C; 154 G; 114 T; 0 other; agaattcggc acgagggcca cgtcgccgtc cccctcgtct gcctcgtctt caccatcatg 60 ggctggtttg gttccaagag ctccgaggca cccgacgcaa catcacgaaa ggaccgtcag 120 aagtgctggg aaagtcggga cgcgtatttc gcatgtctcg atgctgccaa agtcgtcaaa 180 cccggcgagg agggaaacac ctgtgcggac actaaggggg cctatgaggg gaactgtgcc 240 aaaagctgga ttgactactt caacaagcgt cgagtgctcg ctgagcagca gcagggtgtc 300 ctagcccagg cccaaaacca agccaatgct gccaggaaca ggtagaccgc ccctttcaca 360 gcgcatacac gcacaacctg gagcgtaggc atcctccccg gatctcggac ttgtgacgaa 420 aggtcgaatc cttgctgagt ggcggtggca ggatattata gacgatgccg gggtagctat 480 ctacattatg atgaccttca cgcacacgcg gactccccac tcacatgtac tttacaaccc 540 ataatggatc tttcggaaac tttgatttta aaaaaaaaaa aaaaaaaaaa 590 // ID HO747632; SV 1; linear; mRNA; EST; FUN; 671 BP. XX AC HO747632; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_E06_040 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-671 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 35eb2bc4c1b138c2a4fdd535ee132ce8. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..671 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 671 BP; 158 A; 233 C; 162 G; 118 T; 0 other; gtcgcgtacg caccaagacc accaaaaggg cctcccgtgt cctcatcgag aagtactacc 60 cccgtctcac tctcgacttc cacaccaaca agcgtatcat cgatgaagtc gctgtcgtcc 120 catcgaaacg tctccggaac aagatcgccg gcttcacgac acacttgatg aagcgtatcc 180 agaagggccc cgtccgcggc atctccttca agctccagga ggaggagcgc gagcgcaagg 240 acaactacgt ccccgaggtc tccgccctcg acatcaacac caaccccctg gagatcgacc 300 ccgacaccaa ggacctcctc cactccctca acttcgacac catccccttc accgtcgccc 360 agcccgccac gcagcaggcc gagcggggac cccggaggga acgccgcaac gtccccggtg 420 ctggccgctc gtgagcggga ggcttccgtt gcgcttgggc tcctatgcgt tatgactgct 480 atgctccgta ttgctactgc aaagatgacg atgatggtcg gggccctcgg agcgcagccc 540 ccgcatggct gctggtcgtc gctatcgtat cctcacatac acatgtactt gcctttctac 600 gaagactggg cgggcagtct ccttgtaaaa ttgagccatg caagatcaaa cccaaaaaaa 660 aaaaaaaaaa a 671 // ID HO747633; SV 1; linear; mRNA; EST; FUN; 680 BP. XX AC HO747633; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_E07_055 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-680 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 3acb8c793a607694fa7a4ad652762d6a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..680 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 680 BP; 142 A; 260 C; 172 G; 106 T; 0 other; gtccctccat cccagccctg ccaggccaat tgggcctcgc tcgcttcgtt cggcatcccc 60 acatcccttt ccggtccccg aggtcgcccc cctcaacatc cgcaagcctt ccagggacca 120 gcccaatgga tctgctcttc tctcgccccc accaagcacc agcccttcgt cgcgctcgac 180 cacacccacc ccatccatct ccacaccaac gagcctcatg tcgggtcctt ctccacgacg 240 aaaacccgca ttccccaaga tcgcactcaa taccctcaac ctcgggggcg gctccgcctt 300 ttcggggata ggcggtcctc agccccaaga ctcaccacaa cagcccatac gctcccatct 360 gaccaacgag agcgagccga cgattaaacc ccccgacatg agcaccatct cgccacagaa 420 cccgcccccg cacatggtgt cgatggtgga catcgagacg caggtccggg aattcgagca 480 gtggtcggac gacatgcttg aggacgtcgg acggctgggc gagggcgccg ggggcgcggt 540 gtacaaggtg cgcgaccggc ggacgaacgt cgtgatggcg cgcaaggcga tcacgacgca 600 cgaggccccg atgaagcagc tcctgcgcga gatcaaaatc acgtcaagca cggagcacgt 660 caacatcgtc cacttctatg 680 // ID HO747634; SV 1; linear; mRNA; EST; FUN; 657 BP. XX AC HO747634; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_E12_088 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-657 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 509b6c0963932b58f3a3879bb5ff347a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..657 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 657 BP; 154 A; 241 C; 165 G; 97 T; 0 other; gaaaaacatg gcccacgtcc tccccgacct cccgtacgcg tacaatgcgc tcgagccctt 60 catctcccag cagatcatgg agctccacca caagaagcac caccagacct acgtgaactc 120 cctcaacgcc gccgagcagg cctacgccaa ggcctccacc cccaaggagc gcatcgcgct 180 ccagtctgcc ctcaagttca acggcggagg tcacatcaac cattccctct tctggaagaa 240 cctcgcccca gcccagtctg agggcaaggg caacggcggt gtcctcaagg acggccccct 300 caagtccgcg atcgagcaga tttggggcag cgtggacacg ttcaagaagg agttcaacgc 360 gaccaccgcc gccatccagg gctccggctg gggctggctc ggtctcaacc cgaccaccaa 420 gcgcctcgag atcacgacga ccgccaacca ggaccccctc ctcacccacg tccctatcat 480 cggcgtcgac atctgggagc acgccttcta cctccaatac ctcaacgtaa aggctgacta 540 cttgaacgct atctggagcg tgatcaactt cgacgaggcc gagaagcgct acaacgaggc 600 cctcagcggc gccaagctct aggctgctta cgcgagagcg aggacgaaga aaatcgg 657 // ID HO747635; SV 1; linear; mRNA; EST; FUN; 328 BP. XX AC HO747635; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_F06_038 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-328 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 4176efc3fb281383d91e9a88f5318d57. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..328 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 328 BP; 83 A; 85 C; 53 G; 107 T; 0 other; tttaaattgt cgttccggct ggtgctggtt gattttctcc ggttctcttc ttgccgtctt 60 tcgggttctt gtgcttggac cacattgcgc attcatctgc actacgcatt cgttccgact 120 cataatcact tctctttaca catggtttgc acactctcct tggatcactg tagcatttcg 180 ttcgtacctc cgtattgtgt ccattcactg tatccatacc tagaaacacc ccgactggga 240 ttcgccgcat atttgtatcc tacacatcta gacaatgcac aagtgtactc tcttagattc 300 caaaaaaaaa aaaaaaaaaa aaaaaaaa 328 // ID HO747636; SV 1; linear; mRNA; EST; FUN; 347 BP. XX AC HO747636; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_F08_054 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-347 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 4b3a866a0256152f8869721807b0b9c7. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..347 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 347 BP; 60 A; 129 C; 89 G; 69 T; 0 other; gacacacaag agctttccgt cgcggcctgg ctatagagat gagcgcagct aacgagaacg 60 tcctggttgg tgtcatcggt ggcagtggcc tatatcacct cgataacttg acacttttgg 120 aacatgtgaa cccagagacc ccatggggtt tccccagttc gctcatcgca atctgcgccc 180 tcccctcggg caccaaagtc gcctttctag cccggcacgg catcgggcac tcgatcccgc 240 cctccgccgt gccctcgcgc gcgaatatcg cagccctcaa atcgctcggc gtccgcgcca 300 tcctcgcctt ctccgccgtc ggctccctgc gcgaagagct cgcgccc 347 // ID HO747637; SV 1; linear; mRNA; EST; FUN; 513 BP. XX AC HO747637; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_F12_086 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-513 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 1e7b69f070c029ee2f8f567a47b18355. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..513 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 513 BP; 87 A; 198 C; 135 G; 93 T; 0 other; ctcactatgg ccaccgagaa tccctctgcg atcttgccca ccctcgaccg cctcggcgta 60 tctgcggtac ctgccaacgt cgacgcgaaa aaggtcgcac agctctggct cgactccttc 120 gcgaagtaca tccaatccgg cgatatcgat ggcgtcctgt cgcagttctc ctccgacgcg 180 tggtggcgcg acatgctcgc cctcacctgg gccttccgca ccttccacac cgcgccgcag 240 atccgcaagc ttctcgagga caggctcccg ttcgccgccc cgtccgactt ccagctcacc 300 gacgccacgt tcgagcaccc gttttcggac ctcgcgtggg tgctcgtgca gttcgcgttc 360 cagacgaaga tcggcaagtg tagcggcatc gcgcgcctcg tccccaccgt ggacggcggc 420 tggaccggct tcacgttcta caccaacctg gaggacctca aggacttccc cgagaagatc 480 ggcgcgctcc ggaaccacct cccgaaccac ggc 513 // ID HO747638; SV 1; linear; mRNA; EST; FUN; 495 BP. XX AC HO747638; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_G03_019 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-495 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; e3a63da876bc199c9f8e644c91834fed. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..495 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 495 BP; 102 A; 167 C; 125 G; 101 T; 0 other; ccagtctcga tctctcccac catgcttgct tatctcaacg agcagccgtc tttcatgcca 60 ggtgtccgtg atgtgcctcc caccgcgtgc gtgtttactg gtggcaatcg ctcggttact 120 tacccaatag tatcaggcga atttcaacag cccgcaagca ctcaccgggc aacgatgtgc 180 cttctaccca cccgaccata ggcactctcg atgcgacaaa ggacttcgta cgagacttcc 240 ttcgccgact tccatacacg cccccagaac tcccgacaaa cccggagctc aggcgcgagg 300 tcgcagatat catcgcgtcg tggaatgtcg gcgtggattg ggagtacatc gagggcctga 360 cggagactag ctgctccatc gccgagtccg catacgcgca caccggctac gagcaccaac 420 gcctggtcgc gatctacacg gcgtgcctta catacgctga tgaccttggg catcgcaacc 480 tcgaggctct cgggc 495 // ID HO747639; SV 1; linear; mRNA; EST; FUN; 615 BP. XX AC HO747639; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_G04_020 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-615 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 69ccf57cdea105e1c7906ffcfe63ecaa. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..615 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 615 BP; 147 A; 201 C; 138 G; 129 T; 0 other; agctacccca cttagggcgc gtcctctctc gtatcagcca cctctttctc cattccatct 60 cggcttgcct tttcctctca tgcctcctgc tgcaaccagg aactccagga gaaagaaggc 120 agtcaaagac aaagattcta catcgcgtca gacttcctta ctggacgcct tcggtgttcg 180 aacgtcgtcc aagaagacct ctccagccac ctcagctgca cagtccgaga atggttcaac 240 cacgggagac gatgttatcg acattccttc ctccgacgct gaaccgctta tagagttgcc 300 actggattcg tccagcccaa tgacagtaga agaaggcgag agccagaaag atgaagctat 360 catccagcat gtagaggtga agtttggtcg ggccaaccaa gctgggggta gtcgtgacgc 420 acccatagta gtcgccgaca gtagcccgct tgcttctcca gtctctcggg ccaagattca 480 ctccccaaga gtgtcccctc ccccaaaggc tccacctaaa gccctcttct cgatctttgc 540 accgaggaaa cgacccgaag cgcgagaatc atcgccgagc aagctctatg gcaagccatc 600 cactacaccc tccgc 615 // ID HO747640; SV 1; linear; mRNA; EST; FUN; 649 BP. XX AC HO747640; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_G05_035 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-649 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; e904a28b7b8f696d4c2563124ead342f. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..649 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 649 BP; 130 A; 205 C; 209 G; 105 T; 0 other; ttgcttgggg tagaaaatgg cgtcggacag gccctatgtt ttcttcgaca tcactatcgg 60 tggtcgcccc gctgggcgga tcgtcatgca gctattccag gacattaccc ccaagaccgt 120 cgagaacttc cgtgcactgt gtaccggcga gaagggcgtc ggcaaagcag aaaagcccct 180 ccacttcaag ggatgcacct tccaccgcgt gatcaaaggg ttcatggtcc agggcggcga 240 cttcacggcc gggaacggga cgggagggga gtcgatttac ggtgagaagt tcgaagacga 300 aaactttgag gtgaagcata cgaagcgctt cctcctctcc atggcgaacg cgggcgcgaa 360 cacgaacggc tcgcagttct tcatcacctg caacccgacg ccacacctcg acggcaagca 420 cgtcgtcttc ggcgaggtcg tccgcggcaa gaccgtcgtc cgcgcggtcg agaactcgga 480 gacgtcgagc ggcgacgtgc ccgtcgagcc gtgcgtcatc gcggactgcg gccagctcgc 540 gcccgaggac ccgtcgctct cgcagcccgt cgccgccgat ggcgacgtgt acgaggacta 600 cccggaggac caggacccgg tggagggcca ggacgtcagc caggcgccc 649 // ID HO747641; SV 1; linear; mRNA; EST; FUN; 642 BP. XX AC HO747641; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_G07_051 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-642 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; b873e478408907a942871c148f0cbb33. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..642 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 642 BP; 119 A; 247 C; 150 G; 126 T; 0 other; cccctctagt acctaatcca agaggctcgg ctcgccgtct cctcgtcagt ccttacacca 60 tgttcaccct cgcccgtcgt taccgctcta ccttcgtttt ttgctgctct ggcctctgct 120 ctcgtcctcg ccgatgcctc ccacgaccac cgcggcctca accatgctca ccgccgtatc 180 accctcaacg aaaccgatgg ccatgttgag aagcgttttg atggcactcg cctcacctac 240 ttcaacgttg gtgtcaacgc ttgcggctcg ttcgaccatg atagcgactt cattgtggcg 300 ttgaacaccc accaatggga cggtggctcc cactgctacg aagagatcac catcacgtac 360 caaggcaagt ccgcccaagc caagatcacc gacgagtgcg aggagtgtcc gtacgcggcg 420 atcgatctct cgcccggcct cttccaatac ctcgtccccg gcggcctcga cgctggccag 480 gtgtatggaa gctgggtgtt cggcggcggc tcgcccccac ccccctctac caccaaggcg 540 ccgccgcctc ccaagacgac gtcgacgacg catagcacaa cgcgacagcc gaccacgacg 600 tcgctgtcga cgccccccac cacgtcgcat tcatccacca ag 642 // ID HO747642; SV 1; linear; mRNA; EST; FUN; 589 BP. XX AC HO747642; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_G09_067 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-589 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; dfc5d51e98cb4060c382ea566e479ff1. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..589 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 589 BP; 116 A; 191 C; 153 G; 129 T; 0 other; gcaagtccac tggtggtaag gccccccgta agcagctcgc caccaaggct gccaggaaga 60 cggcgacggc ggccgcgacc ggcggtgtca agaagccgca tcgcttccgg cccggaacgg 120 tcgccctccg tgaaatccgg cgctaccaga agtccaccga gctcctcatt cggaagctcc 180 ccttccagcg tctcgttcgt gagatcgccc aggacttcaa gaccgatctc cgcttccagt 240 cctccgccgt catggccctg caggaggccg ctgaggccta cctcgtctct ctgttcgagg 300 acaccaactt ggctgctatc cacgctaagc gtgtgaccat ccagcccaag gatctcgctc 360 tcgctcgtcg cctccgtggc gagaggtctt aagcgcctag cgccccccgg ttctcgatac 420 aggcctggcc cttcgctata gtacactgta ttccgcttcg tcgtattcgc tcgtattgtc 480 tggtcttttc gaagaccttg tattgtaatg cctcaatatg cggtaggagt atgtatacgg 540 tccggtattg gtagatgaaa tcgatatcta tgccccagaa aaaaaaaaa 589 // ID HO747643; SV 1; linear; mRNA; EST; FUN; 557 BP. XX AC HO747643; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_G10_068 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-557 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 8210cb1b6942b637ea0a4f9ad3e73409. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..557 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 557 BP; 123 A; 159 C; 151 G; 124 T; 0 other; gagacatcgt ccccttgctg tatctttaaa accaaaagat gtctgacgct tctgatgaga 60 tccaagtcga cgcccccgtc gaggttgagg cccccaccga ggctcccaag ggcaagcttt 120 ctgtagagga cgctcttcag caagtcctca agaacgccct cgtccatgat ggtctcgctc 180 gtggtcttcg tgagtgcgcg aaggcgcttg acaagcgcca ggcgcacttg tgcgtcctcg 240 tcgagacgtg cacggaggcc gagtacatca agctcatcga ggccctctgt gctgagcaca 300 agatcaacct catcaaggtt ggtgacgcca aggtcctcgg cacgtgggct ggtctctgca 360 aaatcgaccg tgaaggcaac ccgcgcaagg tcgtcggctg ctcgtgcgtc gtcgtcaagg 420 actacggcgt tgagtccgag ggcttgcacg tcctcctcga ctacttcaag aaccgttaaa 480 ttattgtcgg ttcctctgta gctgtagtca gttgtagttc tccagcaatc aatggcatga 540 tatgcacgaa aaaaaaa 557 // ID HO747644; SV 1; linear; mRNA; EST; FUN; 263 BP. XX AC HO747644; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_G11_083 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-263 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; ff94d667df5233c26eb2ecfa88a4c852. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..263 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 263 BP; 88 A; 56 C; 67 G; 52 T; 0 other; ctgagctcgt ttgaggatgc gtgcaagact tacgagctca cctgggccat tcgtctctcg 60 gcggagcgta atcgccaggc caagctggac gccgacaagg cagctgctaa agctgctgca 120 tagaatggta gaacgcgaga ggcatgcacg gcgattgaat aacaatgatt gtacgattcc 180 ccgccttgtt gtacaccata caccgataga gattacaaaa gtggagatgt tgatgtataa 240 aaaaaaaaaa aaaaaaaaaa aaa 263 // ID HO747645; SV 1; linear; mRNA; EST; FUN; 382 BP. XX AC HO747645; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_G12_084 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-382 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; bac2b8d2bae1f1e0caad47137d5f22fb. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..382 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 382 BP; 96 A; 90 C; 103 G; 93 T; 0 other; gtggcgacta ccgctacctc cttctcgaga agagcgccac gcagacggag agggttactg 60 aggttgctct gctgcgtctt gacaatgcga tgaccgagcg tcctgcgggg tgggctggaa 120 acactatcga catcaacaaa ggccgcggca agagctattt gtacctcctg tggaagaccg 180 cttgaagctc tcgttcttgt taaccattga acagctatca aacacttccc gtagaactgt 240 ggcttgccag tagccaggat ggcgatgtat ctgagtgtcc cgaatgtcgt agagttgttg 300 ttgctttgtc gttgtgaatg tcaagtgcgt tagcgttgtc caataaaccc gccgtgtttg 360 agccaaaaaa aaaaaaaaaa aa 382 // ID HO747646; SV 1; linear; mRNA; EST; FUN; 702 BP. XX AC HO747646; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_H06_034 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-702 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 46c71756d4d6d1f718238e5271a5531f. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..702 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 702 BP; 125 A; 254 C; 171 G; 152 T; 0 other; tcccttgacc ctcgcggtcc cttccttcga ccgcagctct ccccgacccg tcttgcaata 60 tgtctactgt cgctctttca gcccttctcg ccctctcggc tgccacttct ggcgttctcg 120 cccagtccgc taccacttct tccttcgtcc cgctcgcctc caagcacttt tcatacccca 180 gtggcatccc ctaccaagcg gattccgatg atggtgttcg tggtacccag cagggttaca 240 acctttgcaa ctccacgacc gagggcgtca actcgctctg ccagaccgcc ttcgtcaacc 300 acctcgacga ctggtgtgtc tgggcgcccc ccaagcccgg ctccaccatc ggagacaccg 360 agggtgagga ggttgcttgg tgcaccaaga agggccgtgg tacacgtatc atccccgctg 420 gcgccatcac cggtgtccag ttcatgaaga cccctggcta cctccagatc gtcggcttca 480 tcgaccagac caagatcaac ctccagtctg atgatggcgg aggagagctc gacccccacg 540 gtgctgatct ccgtggtaac cctcttggcg gtctcgtcta ctcgaacggg ttcgcgagca 600 acaacgggaa caacaacacg ttccagcagg tgatcgagtg gcacaacttc atgggctcga 660 actcgttctg cttcaaggtc tgcgaccccg ctggccccaa cg 702 // ID HO747647; SV 1; linear; mRNA; EST; FUN; 623 BP. XX AC HO747647; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_H07_049 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-623 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 8bfb3650ff9089532ee82d7451a9acc4. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..623 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 623 BP; 101 A; 254 C; 147 G; 121 T; 0 other; ctcgcattcc gttcttgctg cccccatttt cctcgcttcg caacatgtcc tccgacgaga 60 agaacttcac ccactcgctc cccatcccca tcgcagaggg ccgccgccgc tcaggctccg 120 agtcagattc tggctcttcc gactcgggat ccaactctcc cccgtcccct taccccactc 180 cggcgtctgg caccttaccc cgcattgcgc ccttcagtcc gtccacctcc cccatcctct 240 ccttcttcct gagcaaccaa ccgacctctc caaagtctcc tccgtccacc ttcccgttca 300 gaagaggcac aacgggccca agtttctgcg aagacgagga tgcgttcgag gccgacaagc 360 ctatttatca gcacggccgg cgtgcgagtg ctgctgcctg gcccgggcac gaccgctatc 420 cccctgccgc tccggtgcct aacgatcagc aggagcgcgc cgcgggcctg ctccggcggc 480 tctctctggg cggcaatatg gctaggcccc caatcccgcc gattccgaag aagatgccat 540 cgaacgggtc ttcacaggcg cctcgtagca agacgcctcc gaacctgacg tccaactctg 600 ctgctgctgc cgccgccgcc gcg 623 // ID HO747648; SV 1; linear; mRNA; EST; FUN; 722 BP. XX AC HO747648; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_H09_065 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-722 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; f6b8520a1e9494c864ef74007d8d37e6. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..722 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 722 BP; 199 A; 211 C; 188 G; 124 T; 0 other; tttccctcgt cgccgtctcc gtctcccact gacgcaacca tcatgccgaa agcaccaaag 60 gcagcgacga agtcaaaaca cgatcccctt cacgtccaga ttggcgagga tgaagtctac 120 gcgaagtacg gccgtatatc tcagccagga cgccgtagga agtcgaaaac aaaagatgac 180 gacgacgaag ccgcagaagt gatattggat cccaaaacat cacgccgcat ctttgagctc 240 gctcgcgatc agcaggacga gctgggcgag aaggacctgg acgacgagga tgaaattgat 300 gatgatcagc ctgacctctc cgtcccgaga gcgacgctgc cggcagatga cgacgacctg 360 gatgatttgg accagtacga tgacaaggac gacgaggaaa ttgaggagat agaagtcgat 420 gaagatgaca tgaaagcgct agacgccatg ctcccagcaa atgccggcga gcggaggaca 480 ctcgcagaca tcattttctc gaaactggac aacttagaag ccggccagcc agatgctgca 540 tcagagaagc gccatgaccc agaccgtatt cctgacccgg cagccgggct tgatcccaag 600 gtcgttgagg tttacacgaa agtcggccag atgctgaccc ggtataaatc cggtcccctc 660 cccaaaccat tcaaaatcat cccttccctc ccgcaatggt ctcgcatact cgcgcttacg 720 ca 722 // ID HO747649; SV 1; linear; mRNA; EST; FUN; 310 BP. XX AC HO747649; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_H10_066 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-310 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; ceb757572f573f8a63121674b44d1d50. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..310 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 310 BP; 81 A; 79 C; 80 G; 70 T; 0 other; aggtctcgga gactccgtcg caagcgcaga aaaatgcggg ctcgctccaa gtaatcgccc 60 aaacggcttc taggcttgca aataagaatg tcacttcaag cggtgtatta tgtcctgctc 120 ctcgacgtca ggagggtcat gccgactcag tcgcgtcgcc tcgcgccgcc cgcctcaggc 180 ccggggagtg gtgtctcgat taggtcagga cgtcgaggca gttatggggc attgtaacaa 240 tagttcatgc ttgtcacttg tattgcaatg atctacttgc cgactctatc aaaaaaaaaa 300 aaaaaaaaaa 310 // ID HO747650; SV 1; linear; mRNA; EST; FUN; 536 BP. XX AC HO747650; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050044.T3_H11_081 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-536 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 3d1025756694406426afc008b604e2eb. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..536 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 536 BP; 117 A; 155 C; 163 G; 101 T; 0 other; agcactgtac ccagcacgcc cgccacggag ttcggtgcgg acgacggcgc gagcagcttt 60 gacgagaagc gagagaagcg gcgcaagcgc agaaaggcgg agatatggat cacacggcac 120 gtcgcggaga tcgtgtgtcg ccaagagttc atcctcaaac tcgcacgcgc gatgatgatg 180 tttggcggcc cgacgcaccg gctgcaggcg cagatcaagg cgacggccaa ggtgctcgac 240 atcgagctgt cgtgcatgta cctcccggac gtcatgctca tctcgttcga tgacgccggg 300 acgggcacca gcaacatcaa gttcatccgc cagggaagcg cgctcgacat cgggaagctc 360 caagaggcgc atgagcttta ttgggaggtc attcatgaca atgtctctgt caaggacgcg 420 tctgtccacc tagacgacct catgcgccgc aagcagtttt acaagaactg gcaaatcgtt 480 ctcttcgggg gcgcctgctc ttcgtccatt tgcagtgtca gctttaacgg gtcgtt 536 // ID HO747651; SV 1; linear; mRNA; EST; FUN; 650 BP. XX AC HO747651; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_A01_015 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-650 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; fbfd3176e64cefd9857717939875f139. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..650 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 650 BP; 168 A; 177 C; 178 G; 127 T; 0 other; cctccttcag gcgtatatcc cctcgccctt tgcctcctcg tcaacatttc tcagcatgac 60 cgatactgct gcaacggaac ctactcatcc cgtccccgat gccgatgcaa ttattgacaa 120 tgctgaggag gaggaagatt cagaaatcct tcttatgaaa aagcgcgtag aggagatgga 180 acgcgaggcc aacaaactac gtgaactcca atctgccgca gaaaaagcgg aacagagctc 240 ccaaggaagc gaacaagtac ccatggagac tgaggaggac aaggcggcgg cagactcaag 300 gagcgtatac gttggaaacg ttgactattc cgcgaccccg gaggagatcc aacaacattt 360 ccaggcgtgc gggactatca accgggtcac catactctgt gacaagttta ctggccaccc 420 caagggatat gcgtacgtcg agtttgcaga acccgacttc atagacgccg ctctcgctat 480 ggacaattcc ctcttccggg gacgtctcat caaggttact ccaaaacgga caaatatccc 540 tggtttcaac gctcgtggac gagggcgggg tgggtaccgc ggcggatatc gtggagggtt 600 taggggaggc tcgtatggat acaacccata ccgtgggcgt ggcaggggtc 650 // ID HO747652; SV 1; linear; mRNA; EST; FUN; 593 BP. XX AC HO747652; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_A02_016 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-593 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 410155ffc460136345dbdd0bd94fa3f6. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..593 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 593 BP; 78 A; 243 C; 172 G; 100 T; 0 other; tcccatccag agccttgagc caggtgctcc ccacattccc tacacacttc ctatttgcct 60 ttggtgcaaa ccctcccttt ctttttaaca ttaccacaat gaaggccgtc tttgctctca 120 ccgctctctt ccacctcatc ctcatggtcg cggccgtgcc cgcaccccag gccaacggcg 180 gtcccaagtt cgaccccgct ggtgccaaga acgtcggcaa cggccacggc ggccagttca 240 tcggcggcca gtgcctcagc gcggccgact gcgcgtccgg ctgctgtgcc ggcccctcgg 300 gcatctgctc cggcgtcggc gcgcagacgc aggcaggcaa gaccggctgc ggcttcgtct 360 cgggctcctc ctcccccgcc cccgctcccg cccccgcccc cgcgccccca agcaccggcg 420 cgtccaacac gcccgcgaac ggcggccccg ccttcgaccc cgctggggtc aagaacgtcg 480 gcaacggcca cgggggccag ttcattgggg ggcagtgcct cagcgccgcg gactgtgcgt 540 ccggctgctg cgcgggcccg tcgggcatct gctctggggt cggcgcgcag acg 593 // ID HO747653; SV 1; linear; mRNA; EST; FUN; 507 BP. XX AC HO747653; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_A03_031 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-507 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; bae4c8f4c85c96b60639fa0372bf8d9e. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..507 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 507 BP; 73 A; 167 C; 183 G; 84 T; 0 other; gatcccgggc ctcacccagc gcgcatccac aatgatctct ccacacgctt ctatctattc 60 tgtgtccgcc ctgggcgccg gcgctggcac cgcagcggcg ctcgccacgc tcccgcacgc 120 ctatccccgg ggcacggggc cgggcagcgt cgtgtctggc ggcagctccg cgtccggggc 180 gcgtgcgctc tctgtggtga acgacaccgc ggacctccgc gctggggtgc cagtgggcgc 240 cggaggcgca gtgctgacgg ctgcgcagct caaggccgcg gagacggagg gcgagaagaa 300 gaaggatggg gggcccgtcg acccgcccgt gcagttccac tcagactcgg gtgtgcggtt 360 cgacgaggac ggcaacccga tattgccctc cgggtctggc tctgggtctg ccgaggcggc 420 gcgcgagccg gaagcgatcg aggtgccgcc agagtacacc gagatatgat cattgcggag 480 gtgcctgtgt ttgggtttca atggttc 507 // ID HO747654; SV 1; linear; mRNA; EST; FUN; 753 BP. XX AC HO747654; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_A05_047 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-753 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 99f9b5368405009f1a2bfcb1acb0dd68. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..753 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 753 BP; 173 A; 228 C; 215 G; 137 T; 0 other; gtcacgctgc tgttgactgc catccagacg acttccaagt tcatcgcgac gaatgtccga 60 aaagcgcgct tgatcaatct ggtcggcctg gccggcgaga cgaacgtgca aggcgaggag 120 cagaagaagc tcgacgtgct ctcgaacgac attatgatca actccctgcg cgcatcggga 180 aagacggccg tgctcgtatc cgaggagctc gaggaggcgg tggtcatcga ggaccggtac 240 aagggcaagt actgcgtcgt ctttgacccg ctcgatggct ccagcaacat cgacgccggt 300 gtgaacatcg gcaccatctt tggcatctac cacatcgcgc caggatcgaa gggaacgatt 360 gacgatgtcc tgagaccagg gagcgagatg gttgcggctg gttacacgat gtacggttcg 420 tcagctaacc tcgtcctgac cacgggacac ggagtaaatg gttatacgct cgacgcggcc 480 ctcggagagt tcatcctcac ccaccccgat atcaagatcc ccccacgagg gaagatctac 540 tcgttcaacg agggcaactc gctctacttc cacccaccag ttatcaacta cctcaactcg 600 atcaagtacc cgcagagcgg caagtcgccg tactcggcgc gctatatcgg ctccatggtc 660 gcggacgtgc accgcacgct gctgtacggc ggcatcttcg gctacccgga cgacaagaag 720 agcaagaagg gggagctgcg cctactctac gag 753 // ID HO747655; SV 1; linear; mRNA; EST; FUN; 842 BP. XX AC HO747655; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_A06_048 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-842 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; d3e5f15386aa78a2bce7701a7be7c8bc. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..842 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 842 BP; 246 A; 165 C; 173 G; 258 T; 0 other; tgcagaacat tgctttttaa acaactggca gggccttcgg aataaggaag ttataattga 60 ggacacttaa atataagtga ggcagtgatc caggaagcct gtattttaaa agacaaaggg 120 acattcatga attggagagc attgcttttc agggctccag gactcagaaa cttgaaagca 180 atctgaaatt ccagtagagg tgagttccaa tttgattttt ctgggattaa ttcattaaaa 240 gctaaaaact gagccctcac tcataacctg atatgtaatt tcgaggagta tagatgaccg 300 accctggagg ctctgttctg ttgttttacg caaatcttca ttttctagac caagccccac 360 tctggtgttc gggatctgct tgcagacact cgtgatttgt agggtcatcc aaagcagttt 420 tctcacctgc agtcagtttt catcctcagg ggacatttgg taatgcatag aaacattttt 480 ggttgtcaca atgcgggggt gctattggta tctagtgttt agatatgttt ccaaacgtct 540 cataaagcac aggacagctc cacacaatga agaataatct ggcttcgaat gataatactg 600 tgatcaaaat caatgatttc caaagagtag tccccaaact agccacttca gtactggaaa 660 catgttagaa atgcaaattc tttgcctcca tgtccatcca acagcatcag aaacttgagg 720 tggagcccct gcaatctggg tataacaagt tctccaggta actttgatgt gccttagttt 780 aagattcttt ccttattaat gttttctgtt tttcatcatc tatttagaaa gtgacttaca 840 tg 842 // ID HO747656; SV 1; linear; mRNA; EST; FUN; 369 BP. XX AC HO747656; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_A07_063 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-369 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 5522dfc10452344ba18abf29a904d729. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..369 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 369 BP; 96 A; 112 C; 78 G; 83 T; 0 other; tcccttcttg gtcctaggcc atttgccaac cgcaaggacg cgatggacaa gtggctggag 60 cagaacagcc aggagcgcag cgcacctccg cccctagaga gtgtcgagcc ttcgagccca 120 ccgccagatc cttctccttc acctgccccc gcggcaaagc ggatagatga cgcatccctc 180 taagttaata ccttcatcct gctttcgtta tgcatgcttt aatctgaggt tttttgcgat 240 ctgatggagt cgctcacgtt ggacctagca taccacacgc gacagcctgt attccgcatg 300 gtatctatcg tacacccgca acactgctca caataataca gcattgttca caaaaaaaaa 360 aaaaaaaaa 369 // ID HO747657; SV 1; linear; mRNA; EST; FUN; 748 BP. XX AC HO747657; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_A08_064 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-748 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; a7285b514753b7badb26cb6a09c4a6c4. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..748 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 748 BP; 131 A; 228 C; 264 G; 125 T; 0 other; agctatatat cagcagctat cctagccctt tcaccgcaac ccaggaatac ctgaccacaa 60 cttgagcttg aaatatgtcc gaggctgtgt catccgaatc caagtcctcc ccgctccctc 120 ttacgcgaag ttccgtactc gctgcgcaca aagcgataga agggcagatt caccggaccc 180 cgctccttac gtcgtcctcg ctctctgcgt cgctgccggg agcgaacacg ctctacttca 240 aggccgcgct ctctattatc caagaagaga ccggcgcggc gttcgtgccc ccctacgacg 300 ccgcgaacac catcttcggc cagggcaccg cgatgctcga gctcctcgca caggcccccg 360 agccgctcgc ggcggtcgtc gcgcccgtgg ggggcggggg gctgctcgcg ggcacggcgc 420 tcgcggcgga ggggacgggg gtgcgcgtgt tcggggcgga gccggcgggc gcggacgact 480 gtgcgagggg gctggaggag gggaagaggc gggaggaggt ggatccgaag acgatcgcgg 540 acgggctgag gacgcccgtg ggcgtgctga atttcgggat tatcaaggag aaggtcgaga 600 aggttatcac ggtcacggac gaggagatca tcgaggccat gcgcttgctc tgggagcgca 660 tgaagatcgt ggtcgagccc agcggcgcgg cgtcgttcgc tgcggtccgg agcggccagt 720 ttcaggcgtt gggcatcgag gggcccgt 748 // ID HO747658; SV 1; linear; mRNA; EST; FUN; 826 BP. XX AC HO747658; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_A09_079 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-826 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; a0019ed0c5148cf9a3229736b295e467. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..826 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 826 BP; 168 A; 248 C; 192 G; 218 T; 0 other; tcttctctcc ctcccgccgc cagggctcct cctccgcccg ccccaacgcc tcattccctc 60 gagccctcat acctttcttt aatcactcat tcgctcctcc ctccatcctg tctctccttg 120 ctcttttcgc ataatgggcg ctgtctgttg tcgagcccag cccatagact tcgatgggga 180 ggccaacctt tttcacttca tcctcctacg atgcgtcggc aagggtgcct ttggaaaggt 240 tcgagttgtc cagcacaagc agactcgtga actctatgcg ttgaagtaca tcaacaaagc 300 aaagtgcgtc aagatgaagg ccgtcgcaaa tatcatccag gagcgccggt tgctcgagga 360 gattgatcac ccatttgttg tcaacatgcg ttacgcgttc caagatgacg aaaattgttt 420 cttcgttttg gacctaatgc tcggagggga cttgagattc catttggaga gattaggatc 480 attgcctgag gaggtcgtaa ggttctatgt cgctgagatc ggttcggccc ttgctttcct 540 ccatgagcac cgcatcattc accgtgacct caagcccgac aatatccttc ttgacgagcg 600 cggacacgcc catcttaccg atttcaacat cgctgtacac tattctgaac ggcgcctgtt 660 gaccggtgtt gcgggctcga tggcctacat ggcacccgaa atccttgcta agcgtggata 720 tacctacacc atcgattggt ggtcacttgg agtctgcgct tacgagttga ttttcggccg 780 tcgaccgttc cgcggcaaga cgaatagcga cctcacccac agtatc 826 // ID HO747659; SV 1; linear; mRNA; EST; FUN; 699 BP. XX AC HO747659; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_A10_080 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-699 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 91a9a00b477266a176075aac9842dee5. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..699 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 699 BP; 143 A; 177 C; 215 G; 164 T; 0 other; tttttttttt tttttttttt ttgggagatg aatggagttc ctggtgctct gatatatata 60 cattccatcc tagtacagtg tggtgaccaa actgttcgcg ccgctccata cgcgcggtca 120 acgcgaggac gtcgatggcg atacttaaca gacttcgcgg tcaatcaaat tctggcaata 180 cgaaaccaat tcggctgctg gtcgtccgcg agacccaact ccttcctgac cgcgtcgatg 240 tgctccctcg tgagcgggga gtgtagtcta tccgaggtgc tgttgtccct cagggcgaag 300 acccagcggt tgtagacgag ctcgttatcc ttcaccggca agagatccga gcggacgcgg 360 ggaaggtcgg ggagagcgtg gtagacgcga ttgggtatca cgtcactgat gacgatcata 420 atgccttcga tcaggccttc gtattcttcg ggagtgtatg tgcaaggctc aggctcttcc 480 ttgtcagcct cctcgaggta gtacttggcc catcgacggc ggcactggta gtcgagcacg 540 aacccgaagg cgagagtcgg ttcagggggg ggacggtggg gatgaacgac cttaacacgg 600 cgggtgcgcg gacgagcgga ctgagagatg gtggggacgt cagccatggt gagccgcact 660 cgcacttcaa gcctctgttc tcccgaacga aagcaacga 699 // ID HO747660; SV 1; linear; mRNA; EST; FUN; 760 BP. XX AC HO747660; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_A11_095 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-760 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 50bd6bbc9e0dc7a6283cfaad227a39e1. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..760 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 760 BP; 144 A; 249 C; 188 G; 179 T; 0 other; gtcaatggtt tccaccgatc ccgccaactt caccatcgtt ctcaacaacc agaacgtgac 60 accggcgact tcgcaagtgc tcgcggccct cgtcgacggc tctcttggca acatgactgt 120 caacccgccg agcggtggct ggaaggcggg caagggcttc cgcgtgaacc tggtcaagga 180 cacccaggac ttgaacagca tcctggctca gtcgccccag ttcgacatca ctacctccac 240 ttcgagcacc ttctcgactt cggttactgc tactgcggcc tcgggcactc tgactgtgcc 300 tgccacaacc gcaagcaccg cgaacacggg cagtagctcg ggtgccctca atccgagctc 360 gtccgacacc tccaccacgc ccacgaagag caacggcgcg tcgcccgcca tggcggtgag 420 caacggcctc gtcggtgcgc ttgctctcct tggcgccttc ctggcttgaa aatggccaaa 480 acggggccgg catggaggcc gtcatgtttc gctattggga cgttcgtttt gtagttttgt 540 tccttcgatt ccttgccaag ccaatcctct tcattcccgc tgctatagat acattaccgg 600 actttagtac tttagtactc acggcgcata ctgttatctc tgaccagagg ggtggccgtt 660 ttacccattg ctccttggga aagggtcgtc gaatctgcgt tagcctaggg tacatctatc 720 aatctactcc ttccgtactg catccgacct ttgttaccta 760 // ID HO747661; SV 1; linear; mRNA; EST; FUN; 768 BP. XX AC HO747661; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_B01_013 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-768 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 9898ffe90217fc88b0eeaed751a0011e. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..768 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 768 BP; 120 A; 312 C; 191 G; 145 T; 0 other; acagcacgta ctaccccggc ccgtaccacc ctgactacct gtcgtcgcag tacagcatcc 60 cgagcgactc gctgccgttc tcctctgggg acacgtccga cgcggactcg gtctcggacg 120 cgcgcgcaag caagcgccgc cgcatgagca atgactcggc gtccgagccg ccgtcctccg 180 ccgtctcgtt cagctcgtac gcagactcgt tcacgtcgac ctcgtcgtcc gcgtcgcact 240 cgcagcgctc ctcggtcgac ttcccctaca ccccttactc gtcgggctcg ttcaacatgc 300 tccgcggcgg ttcgtggcat cccccaatgc tacccccagg cgacagctcg ccccactttg 360 tgcacccgcc gatgctgccg tccgccgggg ggccggatga ctcgccgatg ttcttccacc 420 caccgatgat ccatggccag gacgacacgg acgcgctctt cgcggcgtat ctgcacccgc 480 cgatgctgcc gcccgacgac tcgccgccga tgagctcgct ccagctccac ccgccgatgg 540 tctccaccga gtggaagatg ccgggccacg gcgagtacga cacgaacgcg atggtcacgt 600 tctgagccac catatgccct cctctcacgc tatcccgcct atgcccctct tttttcatct 660 tttctctgcg ctgccgctcg acgctcgatg ccctcatgtc ccccacgtta cccactccat 720 ctcatatccc cccacaacgg acagacgcgt aacggacgat ccgcgatg 768 // ID HO747662; SV 1; linear; mRNA; EST; FUN; 802 BP. XX AC HO747662; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_B02_014 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-802 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; bdbe55d88006de6874bc81684cd38714. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..802 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 802 BP; 199 A; 248 C; 195 G; 160 T; 0 other; gtcatcacca ccgccaccac ccaaagtacg cctctcgaca tgagccctcc aagtaccaaa 60 cccatagctg gttcacctcg cggctggctt ccacttttca gtatctatga agatagtgtg 120 gtcgctggac gcgccgccgg tgctgacagt gtctacaaga cggctttcga ggtaaaggga 180 tccaccgacg acacggtaat cgataagtac tggaacgacc ccgacaacaa ggacgtcgtc 240 gatgctttca tgaacaacaa gctctgcctc gatgcggctc aaggaaagcc ggaggccatt 300 gcagcttata agagatacgc cgtgcaagac tacttctatc tcgtcgactg gttcaagttc 360 agggtgctgc gccttgcgac cctgccccac gacgatttcg atctcgacgg tcttagcaat 420 gaactcaact ccgtccacaa atccctcaac tcttacgtga aagactggta catcacctgc 480 ctcaagcccg agtcggaggg cgggctcggc cttaacgcag tggacttcca ggtggagcgg 540 accatcgggg aacttgctta cagccaatac cttttgaaca actcccgcag cgacaactgg 600 tacaacctcc acatcatctt gatcgggtgc tattggggat ggtcgaagct ggccctgcag 660 ctgtacaacg atcccaagac ggacaagagc acaatctttt acaagagctg gatcgccggt 720 agcgtcgaca ccagcacgaa tccaccccag atgaccaagt ccgccaaagc gctgtcctcg 780 ttcctcgacc agaacctgca ga 802 // ID HO747663; SV 1; linear; mRNA; EST; FUN; 610 BP. XX AC HO747663; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_B03_029 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-610 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 897ca73fd39a0b9f4fb5d0278057c1af. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..610 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 610 BP; 147 A; 165 C; 170 G; 128 T; 0 other; gtgatcgccg acaatgaagc tcgtgagatt tttgatgaaa ctcaacaatg agacggtcac 60 cgtcgagctc aagaacggtt ccgtcataca cggcacgatc accggtgtgg acatgcagat 120 gaacacgcac ctgaagaccg tcaaaatgac cgcgcgcaac cgggaaccca cctcgctcga 180 ttccctctcc atccgcggca acaacatccg ttatttcgtt ctccccgacg cgctcccact 240 cgacacactc ctggtcgacg atgcaccaaa accgaagaac agaaagaagg atgaggcgcg 300 aggccgtggg cgtggtggac ggggcgatcg cggccgggga cgcgggcgtg ggcgtgggcg 360 tggcagggga ttctaaggtc gatcctacca ggtggcgtgt ggtccttctc ttctatgaca 420 tacccacctg tgttggaggt tgtgggtcgg aggagtggat tgcgtgaggt tcccctgcgg 480 actgccactc tgcctgcccg gtgtattata agcaaaacgg gttctgtaca ctgttgtcca 540 tcccatccaa gttgttgttg ctactgcaat tgtagattcc tttactctca aaaaaaaaaa 600 aaaaaaaaaa 610 // ID HO747664; SV 1; linear; mRNA; EST; FUN; 776 BP. XX AC HO747664; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_B04_030 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-776 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; eb6fd054eca5243a02642e6a1f3a4ce7. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..776 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 776 BP; 131 A; 295 C; 210 G; 140 T; 0 other; cacccttgat actctcgaca tcactgtcga caacgcggac tcgccttcct taacggtcgc 60 gactggggtg atcccagcgc aggatggtcc ccgcgaccgg agtgtgatcg agttctgtga 120 ggcgtttggg aagacgcggg gtatccggca cctcgtcatc cgcaagaatg cgtacctgac 180 gcagcccaac gcaatctaca tcttcgagca gctcgcgaag gccatcgggc actggcgcaa 240 gctggaatct gtgaacgtgg ctttccgtct gagcctttct cccgccgcca acgcgctcac 300 caaggcgctg gccgctgccc cccgactgca cacgctgagt acgcagctgc cgacagtgtg 360 gaacacgacg ttgcttgata ttgcgcagaa cccggcgctc aagcgcgtca tcctcgatcc 420 ggctcccgag ctcgtcggcg cgcacctctt ccttgccgag gcaaagcgcc acccccgcct 480 catggaactc atccacgccg gcacccctcc atcctcgccc cgccccattc acgctggtat 540 cgtcgccagc gtacgcgcaa gggcgtccag cgccgctgtc atgcacaccc tgcctatgtc 600 catggcccca tcaacggtgc ccacatgggg ctacgagcgc gatgtcccac caccgatgtg 660 tcccccggtc tcgcgtcgtc ctagccaggc agtctacgcc ccgggctcgg tcgtcggccc 720 gtcggcatat gcttacgcac cccagcctgt cgccggccca agcacgtcct atcccg 776 // ID HO747665; SV 1; linear; mRNA; EST; FUN; 751 BP. XX AC HO747665; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_B05_045 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-751 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; c3db115e3bf90cbecbb9a10932d76af1. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..751 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 751 BP; 163 A; 278 C; 197 G; 113 T; 0 other; ggtaacgacc cgccatgggc ttcttggaac ttgggtgtct tcttgtgcat aaggtgttcg 60 ggcatccatc gctcaatggg cacgcacatc agcaaagtga agtccgtgga ccttgatgtc 120 tggacccccg aacagatggc ttcaatacaa aagtggggaa accggagagc aaacttgtat 180 tgggaggcac acttgagaca aggccacatc ccagctgatc acaagatgga atcatacatc 240 cgctcgaaat acgagtccag acgatgggct cgtgaaggac agcccccaca agaccctgcc 300 gtactagacg gcgatgcccc tgctcccgag gccgctcctg ctcaagccgc gccggcaccg 360 gtcgcacccc caacacatgc ctctcgcgct tccatctcct ccgtcgacgc cgcccgcacc 420 cagcagccac accagctact ctccaccgcc gtcgctgcca gtcgccagac cgccgccccc 480 gccctaaccg ctccccagca gcaacgagct gcaccacaag cccagcaacc acaagcccag 540 cagcaacccc agtcggccgc ggtggacctg ttttcgctcg acttccacgc acctgcggcc 600 gcacagccgg ctcagcagga gcagcagccg aagaaggacg tcaaggcgga gatcatgtcc 660 ctcttctcca ccgccgcgcc tgcccagccc aacccgatgg tctacgccca gcagcaacag 720 caggcggcgg cgttcggcgg tgctggcgcg a 751 // ID HO747666; SV 1; linear; mRNA; EST; FUN; 736 BP. XX AC HO747666; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_B06_046 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-736 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 0e5117e304eba833beec21b6e5ab4a36. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..736 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 736 BP; 152 A; 252 C; 178 G; 154 T; 0 other; tcttctcccc cctcggctgc gtgccccacc cgccagcatg actactcctc atgtaatagc 60 atcctcttcg ggacaccatt cctcgcagtc tgctacttcc aatggcgtcg tgggcaatca 120 cttccgggtc ggaaagaaga tcggagaggg ctctttcggt gtcgttttcg agggcactaa 180 catgctcaac aaccagcctg tcgccatcaa gtttgaacca cgcaagtctg aggcacccca 240 gttgcgtgac gagtaccgat cgtataggac tttgaatggc actccgggtg tgccacaggt 300 ccactacttc ggccaagagg gcctccacaa tgtcctcgtc attgacctcc tcggtcccaa 360 cctcgaggat ctgttcgaca tgtgtggccg caagttttcc atcaagacgg tctgcatggc 420 tgcgaagcag atggtgaccc gtgtgcagtc tatccacgaa aagtccctca tctatcgcga 480 tatcaaacct gacaactttt tgattggtgt accgggcacc aagaccgcca ataccattca 540 tatcatcgac ctccaccctt ctagcgtcgg cccacggtgg cactcctcat acccctcaca 600 gggatcgcca ccgggaccgc gagcgggcgc acaggtcatc acgcaaccag ctccaggacg 660 gtgcctcacc atcgtcgccc ctggtgctgg ccccgacgcc tgcgcacgtc aagaacgccc 720 gccgtccaca cgacgc 736 // ID HO747667; SV 1; linear; mRNA; EST; FUN; 751 BP. XX AC HO747667; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_B07_061 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-751 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 5082fdb7048da7fc632d3ee81608b98a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..751 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 751 BP; 174 A; 191 C; 232 G; 154 T; 0 other; agaacatctt cttcagcgtg gtacagcata ggtacgacag taataagtat aacgagaaaa 60 cacaaagtcc aaaggaatca ataatttagt tcggcgtata tggtatcgtc cgataagtcc 120 gtggcccaca caaacgttct tgactccgaa cccccgtcat ccctggtaac ttactaaact 180 caagacccaa tctttagcgt gatgttcagc gtctccagga acggcattag cccagcccct 240 atcagcgaga agtgtgcgac gttcaggctg acctcgctgc caggcacgaa gtgcgagggg 300 acggtgacgg tgaagttctc gtagggcggc ttccagcccg cgctcgtcag ctgagggctg 360 tatggcccgc tgtaggcgag gttgcccatg acttccgtca cgtcggtctg gttgcaggct 420 ccgttgcacg gccagaagcc gatggcgatg gcaacctctt gcgagctgct cagggtcatg 480 ggccggtcga gttcgacggt gatgttgcta ccaggcctga cggtcgacca ttcagcgggc 540 gcggtgatct gacagcgctg agcgaacgca gaggcgacga ggaaggcaag cgcgaagagg 600 taaaacagca tggtggcgta ggttgtattt ggcctcgaag atgctgtggt ggggtgctat 660 gtaaagagac ggctcgaccc gaaggaaact tgaggaagcc attgcgcgga ctaaggcaca 720 gcttgtcctt cgcgagaatg cgttgagctc g 751 // ID HO747668; SV 1; linear; mRNA; EST; FUN; 737 BP. XX AC HO747668; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_B08_062 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-737 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; a455511f88326f85f6a1bfed733efc7c. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..737 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 737 BP; 147 A; 241 C; 199 G; 150 T; 0 other; gagaacaact ggtccatcag caggcgcgcg tcgtacttcg cccaggtcct ctcccagttc 60 atcgagaaag ctctttccga tgctgtcccc ggccttaata tcaagaccct ctccacgttc 120 ttgttccagt cccacgagcc gtcccatgac ttcctccaga agatggcaca gtctaaggct 180 ccccacgacc agctcgtcgc ggacatcctc ggtttcgctg tggcttcgtc tgtcagtctt 240 tcaaagaccg ccgctgagat cgtcgacttc taccttgggc ctgaccgtgc ggcggagcgc 300 gctgaagtgg tcaggctcgc gaagacgaca gatgcggcca gccgcgagct cttgaagggg 360 tatgttaggg aggcacaacg gctggctcct cagttcgcgg ccgttcttcg cgaggatgct 420 gcctctgcaa gcgcgtcgag ttcccagccg aagcagacct tggtgctgga cttgaagaca 480 gcgcttgtga acccgggcga tttccccgac cccaagaccg tcaaccctcg ccgcccggtg 540 cagtcctatg acatcctgca ggaggcaagc ttctacaaat cccttggaag ctctcgcggt 600 gaagagatcg tcgtcgagat cgtcaaaagt gtcttccaac tcccaaacct ccagcacgcg 660 aagggcgctg ccggcaccat cagcaggctc gagacgcaga agttcggcgt cccgttcgac 720 atgtacaccg ataacat 737 // ID HO747669; SV 1; linear; mRNA; EST; FUN; 808 BP. XX AC HO747669; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_B10_078 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-808 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; d3574be6cd1986c56e9b41900aa3cee9. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..808 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 808 BP; 161 A; 271 C; 226 G; 150 T; 0 other; tagaacacta gagccgcctc gaatagactt ctaagacaca ccctttctgc tctccaatca 60 tgaagtttgc aactgaggaa gagcttgccg gtcaccacgc cgccaccgtc cgcggtgcga 120 tcgagggtat cctcgccggt ctcgccatct ccctccccag tatgtggtac gctaaccgcc 180 gctggcccgc cttccgcgcc ctccctcccc agctcaaggc ccttggcgtc gtccttatcg 240 ttgctcccac ctacgccatc cagagtgagc gtcgtggtgt tgaattcgat gagtccacct 300 ggactggcgc tggcaaagcc ctcctcgacg acaaggagcg cgaggaggag aaccgctggg 360 aggcgctcag cagtggggag aagatgaagg attgggcgat gcgcaaccag tacaaggtca 420 tcgtcggcag ctgggccgcc agcatggcca tcgctgctac tatcgtcatg cgtaaccgct 480 accagacgac gccccaaaag atcgtgcagg cccgtatgtg ggcacagggc ctcaccatcg 540 gtgtcctcat cgccgccggt gtcctcaccc agactcagcg caagcaggcg gcggctaccc 600 gcagcatcga ccactcgtgg gccgagctcc tcgagaagca ggcgaaggag gaggagaatg 660 agctcaagct ccaccagctc gctcaggcgc agccccagcc gcactccgca tgagcgctca 720 gcactgagcc gttctgtctg tcgttggtct atccgagtgg gtcccttcat gatgtccatg 780 acgaattgta gcaccatctt accgcatt 808 // ID HO747670; SV 1; linear; mRNA; EST; FUN; 380 BP. XX AC HO747670; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_B11_093 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-380 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 1135280004f7358d23cadd9ecaab371c. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..380 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 380 BP; 97 A; 103 C; 89 G; 91 T; 0 other; tgaaaagctc gtagttcgcc ctggccgtca actgttcctt tgacaaaatt gggacttcat 60 ccaccgttct ttcttgtgct ttcaggagtt cgggaggtca tcaaaacctg gtttatgggc 120 catcgtgcgt gcataatgcg cgaggcttcg ccgcaatcag gcccaccata gtctgcctgc 180 atccacttca cagattccgg atgcgcacgc acggtcattg cgtctaggac gccgtcaaac 240 gaatacctct ccatgcctcc gtgggaccgg atgctggggg caatccttcg tagcgtccca 300 tgtcgttctc attctccaat cggactttgt ggccagtgtg cggctcagaa aaaaaaaaaa 360 aaaaaaaaaa aaaaaaaaaa 380 // ID HO747671; SV 1; linear; mRNA; EST; FUN; 550 BP. XX AC HO747671; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_B12_094 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-550 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; a13556af26ca9dd096e74b94fb234fbb. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..550 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 550 BP; 116 A; 159 C; 151 G; 124 T; 0 other; gagaagacat cgtccccttg ctgtatcttt aaaaccaaaa gatgtctgac gcttctgatg 60 agatccaagt cgacgccccc gtcgaggttg aggcccccac cgaggctccc aagggcaagc 120 tttctgtaga ggacgctctt cagcaagtcc tcaagaacgc cctcgtccat gatggtctcg 180 ctcgtggtct tcgtgagtgc gcgaaggcgc ttgacaagcg ccaggcgcac ttgtgcgtcc 240 tcgtcgagac gtgcacggag gccgagtaca tcaagctcat cgaggccctc tgtgctgagc 300 acaagatcaa cctcatcaag gttggtgacg ccaaggtcct cggcacgtgg gctggtctct 360 gcaaaatcga ccgtgaaggc aacccgcgca aggtcgtcgg ctgctcgtgc gtcgtcgtca 420 aggactacgg cgttgagtcc gagggcttgc acgtcctcct cgactacttc aagaaccgtt 480 aaattattgt cggttcctct gtagctgtag tcagttgtag ttctccagca atcaatggca 540 tgatatgcac 550 // ID HO747672; SV 1; linear; mRNA; EST; FUN; 112 BP. XX AC HO747672; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C01_011 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-112 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; e292d08add3cdb58e4673794d4c4a4ff. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..112 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 112 BP; 29 A; 28 C; 15 G; 40 T; 0 other; tttttttttt tttttttttt tttttttgat ctaggaagtc aaccccaatt tccacaatca 60 aaagtgtgcc acgcatacaa cgaacacggc cccattgaca tccgaaagcc cg 112 // ID HO747673; SV 1; linear; mRNA; EST; FUN; 797 BP. XX AC HO747673; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C02_012 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-797 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; c1abb610c4658406c6dc3f5658332f2a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..797 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 797 BP; 246 A; 173 C; 250 G; 128 T; 0 other; ggacaccatg gcagcacgca agctgcaaac tgagattgac cggaccttga agaaggttgg 60 ggaaggtgtc gagctcttcg agagcatata tgacaagatg caggcgtcga caaaccagac 120 acagaaggag aagctggaga ccgacttgaa gacacaaata aagaagctcc agcgattgcg 180 cgaccaaatc aagacctggg tagcgagcaa tgacatcaag gacaagtccg cattgctcga 240 caaccgcaag ttgattgaga cgcaaatgga gaagttcaaa gcatgcgaga aggagatgaa 300 gacgaaggcg ttctcgaaag agggcctcac ccagtccgca aagctcgacc cgaagcagca 360 ggagaaggtc gacaccatgt catgggttca gtctatgatg gacgagctca tggtccaagt 420 cgagtcggcg gaggctgaga ttgagacgct acaaggaggg gggaagaaga agaagtctgg 480 aggcgctgcg gctgagcggt tagagggact ggagaggttg aacgagcggc gaaagtggca 540 catcagccgg ctcgaactca ttctccggtt actggataac ggctcgttag caacagagaa 600 ggtcatctct atccaggacg acgtgaaata cttcgtcgag agcaatatgg atgaggactt 660 tgaggagtat gagggcatct acgatgacct caacctcgag gaggaggaag agaagttccg 720 aatgccgaac gacgagagtg acagtgagga ctcggaagaa gcaagtgaac cagatttgcc 780 tctacgaacg ccatcca 797 // ID HO747674; SV 1; linear; mRNA; EST; FUN; 709 BP. XX AC HO747674; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C03_027 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-709 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 9f5b04588225743ef7d06cfb139d3376. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..709 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 709 BP; 136 A; 225 C; 195 G; 153 T; 0 other; tttttttttt tttttttttt ttccgacgaa ccaaaaacgt tgcagaaaga acggtataga 60 gtagcagtgg ggatataaac aaacaattga gaacgaacca agccgccagg gcaattgtcc 120 cactctcaga gccgcgacaa gtcgcgtccc cagatacccc gacagaccca atcgatcatc 180 agcagcgccc tcgtgcgcat gctgacctgc tcgctccagt acacgctgcg ccacgcgtac 240 atcgccgcga gcccgcccat gaagctgaat cgcccgaggt cgaacacggc cgcgttcccg 300 atgtatgcga gcgagcccag gtgcgtgtac cggaacggct gcgcgacggc gtcgtccgcg 360 ccctcggtcc cgacgccgtt cgcctcgagc acctcccggc gcgcggcgat tttcgagagc 420 ttcttgccga ggtacttacc ctgctgcgac gcaacctgtg cggttgcggg taacgccgtg 480 atcttgttgc cgacctcctg aaggaggacg gcgagctcgt tcaagttcag ggagttgtcc 540 gagtccgagt cgtacaggtc gaacagctcg cggaccttct ggagctggtc ctccgccatt 600 gggatccgct tcttgatgcg tgcgaccatg atctcccatt cttcgaagtc gatcttgccg 660 tccttgttct tgtccgactc gtccaccagg tccaggaggt agctcacga 709 // ID HO747675; SV 1; linear; mRNA; EST; FUN; 671 BP. XX AC HO747675; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C04_028 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-671 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 352e137e7d6225e7b2a37371bc2ca907. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..671 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 671 BP; 148 A; 215 C; 187 G; 121 T; 0 other; ggttcgtctc tgaccgatga ggaccgtaac aggaactcat cagacgaggt tcccatgact 60 ccctctccag cacctactgc atcgaacggc tatacaccag cgcaccctcc cgcaatgcca 120 ggcctgctcg cccttccacc attccccgaa gacgtcccga cgcacccgtt gctcgtcgtc 180 gactaccagc tgatcaagaa gcacgaccag gcggaggtcg acaagctctg gaaggccgcg 240 accgagcttg gcttctggta cttgaaaaac catggcgtgg acgaggacgt caacaagatg 300 ttcgagatgt gcgcggagac gatgcgcctc ccgctcgacg agaagatgaa gtacgagcag 360 ggcgacgccg ggatgtcgtt cgggcgtgta ctcacctcct gattcccgtt cccattgatg 420 ttctgcagcg tcgctgatct ctacatgcag atataaagcc gcgggctcga ctgccaccga 480 tgagaccggc gccctcgata cggtggagtt tatcaacgtc gcgaaggacg acgcgctcgc 540 gtggcccggc aaagtccacc gcgagtaccc ctcgatggtc aatgcgcgca tggagtccac 600 catcaggccg ttcatcaaga aatcgctggc cgtgaacaaa acgctcctgg gggtcttgaa 660 cgacacccgg a 671 // ID HO747676; SV 1; linear; mRNA; EST; FUN; 742 BP. XX AC HO747676; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C05_043 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-742 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 0703924633cc355c3a49145cc74962fa. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..742 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 742 BP; 142 A; 281 C; 193 G; 126 T; 0 other; actcccgagc actttcgacc tgcggaagag cagcgcatcc aaaacgtctc ctagaggaac 60 cagctagaca cagaacagca caaatgtctc accacgacgt cctcggcgct gtcattctcg 120 cccgcaacgg cgaccgcacg gagtgctacc agcacccggt cactcagaag cccagcacca 180 ctcgcagcac cccgctgggt gaggtccaag cgcaccagct cggccagtac ctccgcggcc 240 tctatcttga cccttcctcc gcttcgcaca tccgcggcat ccactccgac ctcgtcgacc 300 tcactgaagt cgctgtccgc gtcaaggtcg gcggcgaggg ttcggcggtc ttcgactccg 360 ccaccgcgct cctccaaggt ctcttccccc cgaacccggc caacaggatc acccttgcga 420 acgagaccac catcgtcgcg ccgctgggtg gctaccagta catcccggtt gagacggtcg 480 agcccagcaa cgaccgctcg ctcgagccct ggactgactg cccggccttc cagaagcacg 540 ttgccaagat ccatgcgtcc gccgagttca aggagactgc gaggcaggcc cagccgttct 600 tcaacgcgct cagggacttc gccttcggcc gggacctttc gctcgagaac gcgtacaacg 660 tctgggactt cgtgtcgtct tcactggttc acaacaagac gtacgcgcac cgtctcccgc 720 cgacgttcct tgagcaggcg cg 742 // ID HO747677; SV 1; linear; mRNA; EST; FUN; 714 BP. XX AC HO747677; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C06_044 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-714 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; c6d5c311fc60f435473b5e1309450584. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..714 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 714 BP; 143 A; 260 C; 160 G; 151 T; 0 other; ggacactcac ttccaactta gacgacgcat tcccctccat ttcgcatcat ttctcattgt 60 tctgacgtcc tctgttctat tcgcactgta gccatgagca agcttaacgc caacgccttc 120 agtttcgtcc ctggtcaatt tcgcctctct cagcagcctc aacagcccgc acaaccgcct 180 cctcccccca tagagcgacc tcctcagaca gaagcacctc ctcctcctcc aactatctcg 240 ctaaacatcg gtggggccaa acctgcgcct ctccctgctg ctgcaccgtc cccacctgct 300 actatttctc tgaacatcgg cggatccaag cctgccacgc ctcctgctcc cagtgctccc 360 agtgctcccg ctcctcctcg cgccccgccc aaggttgatg cccctgggca cgagcctgtt 420 aaggctgtcc ctgtccccgc cgcctccacg tccaatgctg ccggctccaa gtcgttcact 480 ctcgagcgcg cgaaaacaga ttccactgcg atcgcacacg aggtgcgcaa cgctgtagac 540 caggaagtgc taaaagatct gtacggaaac gtcaaggagc acctgaacat cgtcttcatc 600 ggtcacgtcg atgctggcaa gagcacgatg ggcggcaacc tccttttctt gtccggcatg 660 gtcgacaagc gtacgatgga gaagtacgag cgtgaggcga aagaggccgg ccgc 714 // ID HO747678; SV 1; linear; mRNA; EST; FUN; 783 BP. XX AC HO747678; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C07_059 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-783 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; bb82aeba8ce60033954ae59f7ae6959a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..783 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 783 BP; 201 A; 216 C; 227 G; 139 T; 0 other; gaggattgtt cctccgccat taccttagtg ggcaaactag ggatcatcta cctatcggag 60 acgatgctgg gccgaagggc aatctacagg actccatcaa cttcgtactc accaacttca 120 tcgagatgaa caagttgtgg gtacgattgc aacatcaagg tcactcaaga gaccgggaaa 180 agagggagat ggagcggaag gagctccgca ttctcgttgg cacgaacctc gtccgcctca 240 gccagctgga gggtgtggac ctcgatatgt atcagaagca aatcctacca agcgtcctcc 300 agcagattgt tgtctgtaaa gacgttattg cacaggaata tctcatggag gttgtcattc 360 aggtcttcac ggatgagttc cacctctaca cactcggtcc cttcctctcc gccaccgcgc 420 aactgcatcc caaggtcaac atcaagcaga tagtcatcgc gctcattgac cgccttgctg 480 cgtacgcagc acgggaggct gagagtgagg atcccgagga gacgaaacgg caagaagaag 540 ccgcagcacg tcgcttggct gagagggtca agcaggcccg cggtgtgcgg caaaatggcc 600 agaacccctc gtctcccgtc gccgagtcca catcaaccct ccacaacgag tggggttcgc 660 ccccaacgag tcctaccgtt gccgagaagt cagaagaagc tccggctccg gctacgaacg 720 ggaatgggaa cgcggaaggc ggcgagacag agagggacga gaaggggaaa gagaaggagg 780 cgg 783 // ID HO747679; SV 1; linear; mRNA; EST; FUN; 599 BP. XX AC HO747679; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C08_060 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-599 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; e4e500565ef0147ced6da56315180e7e. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..599 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 599 BP; 95 A; 192 C; 215 G; 97 T; 0 other; gggcgcttct ccgcgggctc atcgaggcgc acggccgcct caggtccgag ggctccatgc 60 gcttcgacga ctttgtgcgc ggtaagggcc agggcctcgt catcaacctc ttcggcccgc 120 ctggggtcgg gaagacgctc tctgcagagg cgacgagcga gcacctccgg cggccgctgt 180 acgtggtggg gagcggggac ctcgggacga cggcaggcgc actcgacgac gcactgcggg 240 tcacgttcga gctcgcggcg gcctggcgcg cggtgctgct gatcgacgag gcggacgtgt 300 tcctcgagca gcgctcgctg cacgacctcg agcgcaacgc gatggtcgcc gtcttcctgc 360 ggcacgtcga gtactaccgc gggatcctgt tcatgacgac gaacaggatc ctcacgttcg 420 acgaggcgtt cctgtcgcgc atccacgtcg cgctgcactt cgacgagctc agcgtcccga 480 cgaaggtgca gatttggcgc gggttcctgc gcaaggccgg gttcgaggag gcggagctca 540 aggagagcct gctggtgcag ctcgcgaggc gcgacatcaa cggacggcag atcaagaat 599 // ID HO747680; SV 1; linear; mRNA; EST; FUN; 629 BP. XX AC HO747680; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C09_075 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-629 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 128642f93b1d46e12517da1759294ea3. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..629 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 629 BP; 102 A; 225 C; 201 G; 101 T; 0 other; gacgcagctc acgctgttct tctgctcaca ggtgtgtgca tcgtttgcgc cgcgcgcccg 60 tcacgcgcac gcctagcagg cgcgcgcgca caggctcgga gtaccgggat gcggtgtgat 120 gctgacagta ttctgtgccc ctccgctctg cccgcgcacc atcagagcgg gatcagccac 180 tcgctcgact tccccatcga catcgacgtc gacatgccgc ccgcgctcga cgtgctcatc 240 gcgtcctgcg tgccgcacgc gcagcggagc gagtacgcag agaccgagac gctgttctac 300 ccgccaaacc tgccgctcac gaccacgctc gagctcgcga accacccgat cctcgaagcc 360 gtgcggaata cgctcttccc agcgctcccc gtgggccact acctcaccgc gctgcgggac 420 aagctggagg tgtggccgtc ggggaacgcg ccgctcatcc agccgcgccc gatcgacaac 480 cgcgtcgcga cgatcctgat cacgctcccg gtgaagttca agggcggggt gcttgtcgtg 540 cgcggcacgg acgggctcga ggagcgcttt cacgggcgcg gaggaaggcc aggggacctg 600 gagtggacag cgttcatgtc ggactgcga 629 // ID HO747681; SV 1; linear; mRNA; EST; FUN; 812 BP. XX AC HO747681; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C10_076 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-812 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 17db2f2641fb5feb2b0c7e193da8db58. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..812 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 812 BP; 172 A; 268 C; 211 G; 161 T; 0 other; gacgcagagt caggtccaca gcccttcgaa aatggtctct acgccgttag tcccctacga 60 tcccagtgcc ggcaagacgt atgccaacgt cgacccgtcg aacattgccc gtgtcgagat 120 atttcctccc atcggtattg ctcgtgtcgg cgactctggg acttgggcga acggacaacc 180 tgaccccggc tatcctgaaa tcgagtattt ctacggccct gaagctcctg ggctcgatga 240 ctaccccttt ggatcgttcc gggactccca gcgtcgcatc aagcgtcagg ctgcacggtt 300 tcgcgtgtac gcatacgaca gtcaagggaa ggtccttggg gagatcaaca acgctcagaa 360 ctaccgtctc acttggactg tccacgtcgc gaataagaag gctgcgaacg tcctgtttca 420 tggacgccta accaaaccag agaccgacct acgtaacccc gacgttgacc ccgtcgatca 480 tccttccggc gacccgtttg atttgtctct ggagtcccgg aagaagctca tcatcgaccc 540 cggcgagaag accatcgtgc gcgacggtgc caaccccaag ccagtccagc tcaacggcaa 600 gttccaaggc accgcagaca agcccgtcga cgtcaatctc ggcgagctgc gcaccgacag 660 ccatggccgc ctcgtctttc taggtgggag cggccacgcg cgttccgtcc agaccgactt 720 cctcctcaac gaccagccga acatcatctc cgagttcgat agcgtggact ggtacgacaa 780 cgtgtgcgac ggatgggtct ccgtctccat ct 812 // ID HO747682; SV 1; linear; mRNA; EST; FUN; 777 BP. XX AC HO747682; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C11_091 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-777 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; d7a06feabcd45954ff951e135d8aa019. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..777 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 777 BP; 154 A; 253 C; 178 G; 192 T; 0 other; ggcgttcgcc aaccaaagca attttcgaga ccacgttacc catgggtgta ttccaggtcg 60 gggggcatat cttctcgagt atggccatct cgcgcataca cgttagcact gtgcacacca 120 tcaaggcgct atctcccctg ttcacagtcg ccgcttacgc gctgctgttc ggtgtcagct 180 actcgtccaa gacgtatgtc tcgttgcttc ccctaaccct cggtgtcatg ctcgcatgca 240 gtcgcgacgt ggataggtcg agcgcgatcg gtatcctctg cgcctttggc tctgccatta 300 tcttcgtgac ccagaacatt tacttcaaga aggtcgttcc ctccaatggc ggtcaatcct 360 cccacaaact cgacaaattg aacctcctgt tctactcgtc gagtatggcg ttcgtcctca 420 tgatccccat ctggatgttc tacgatctcc ccgtcttgtt ggccgcgaac gaggatcaca 480 tcgtacatcc cacccacggc catgagaccc cgcactcggt cgcctattac ttcatcgcca 540 acggcacggt ccactttgcc cagaacatca tcgcatttat tatccttgcg tcgacctcac 600 ccgtgaccta ctctatagcg tcgctcatca agcgcgtcgc tgtgatctgc atcgccatcg 660 tctggtttgc gcagtccgtc caccccatcc aagcggtcgg tatcacgatg accttcgtcg 720 gtctgtacat gtacaacaac gcgaagggcg acgtcgagaa gggcgaacac aagatgc 777 // ID HO747683; SV 1; linear; mRNA; EST; FUN; 708 BP. XX AC HO747683; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_C12_092 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-708 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 9af60a0eb087cb4554984bd2574002cc. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..708 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 708 BP; 140 A; 228 C; 206 G; 134 T; 0 other; gccccatcca agagcgtctc cccttcgtcc acttcaccct ccactagtac tactcgcgac 60 tgcaaaaatg gcctcagtca aggtcggcga cgagatcccc gcgggcgtgt tcgtccacgt 120 cccgttcacg cctgagctcg aggagcacag cgcatgcggc atccccacga agctctccac 180 tgacgcgtgg aagggcaaga aggttgtcct cttcgccgtc cccggcgcct tcaccccctc 240 gtgccacgcg aaccacgcgc cgccgtacct cgcgcaggtg cccgcgctga aggcgaaggg 300 cgtggacgtc gtcgcggtcg tgtccgcgaa cgacccgttc gtgctctcgg gctggagccg 360 catcctcggc ttcaaggagt ccatcctcgc gctcgcggac cccgatgccg cgtggtccgc 420 caagctcggc ctcaccgtcg acctctcagg gctcggtatc ggcttgggga agcgcacgac 480 gcgctacgcg cttgttatcg acgacttgaa agtcacgtac gttggggtcg agcctgaccc 540 gactaaggtc actgtttcgg gtgtcgacgc ggtgctcgct gcgctctgaa ggggtggcag 600 tggttgacgc agaagaagat agacaaggca tagcaaggtg tcgtgttgta cgttgcaatg 660 actttccatg taatacagaa ttgtgaacct aaaaaaaaaa aaaaaaaa 708 // ID HO747684; SV 1; linear; mRNA; EST; FUN; 783 BP. XX AC HO747684; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_D01_009 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-783 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 875ee5c1470c81495122c3d564b3af73. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..783 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 783 BP; 160 A; 279 C; 196 G; 148 T; 0 other; gaaagactac acgtcgccca cgtcgacgtc ctcaaaacaa acccgcagcg gtccgccatg 60 tcgtcaaaac tcccctccgt cctcagtgct accgacgagg agatacagct cctcctcgcg 120 gcccagtcgc acattggcag caagaactgc gacaagcaga tgcttcccta tgtctggaag 180 cggcggtccg acggtatcca catcatcaac attggcaaaa cctgggagaa gctcgtcttc 240 gctgctcgta tcatcgccgc catcgagaac cccaacgaca tctgtgtaat ctctgcccgc 300 ccgtatggcc accgtgcagt cctcaagttc gccgcgaaca ctggcgcgca ggcgattgct 360 ggtcgcttca cccccggttc cttcaccaac tacattaccc gctccttcaa ggagccccgc 420 ctcatcatcg tcaccgaccc tcgcgttgac caccaggcca tccgcgaggc atcctatgtc 480 aacatccccg tcattgcatt ctgcgacacc gatgcacccc tgaagttcgt cgacgttgcg 540 atccccatca acaacaagtc gcgtcactct gtcggtctcg cttggtggct ccttgcccgc 600 gaggtccttc gcctccgcgg cacgatccct cgcacttccg atggctggaa cgtcatggtg 660 gacatgttct tctaccgtga ccccgaggag gtcgagaagc agcaacagga ggaggccgcc 720 cagaaggctg ccgctgccag tggagagcag gcggaggcgc ccatcaccga ctgggatgtc 780 acg 783 // ID HO747685; SV 1; linear; mRNA; EST; FUN; 517 BP. XX AC HO747685; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_D02_010 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-517 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 69d95cf83f6c40f253c9f04416589a5c. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..517 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 517 BP; 126 A; 156 C; 137 G; 98 T; 0 other; ggtcctctcc actgcggccg actacacatc taacggcctc ctcaccgccg ccattgctta 60 cctccagaca gtgcggcttc aacggcgacg actgcacgct catcgagacc acgctcgtga 120 acccgaccgc ggcaggctcg ggctcctcga gagacatcag cctcatccgc ccacacgcgt 180 tcagcgtcac gtcgggcttc ggctattaca atgggtgtga caacaccggc gcggactgca 240 agagcgggag ttgcgacacc gcgttcttca agccggacga cacccatgtc caggtcgcat 300 gccagaagaa cgacatgaac ttggtcatca cgttctgcga gtagagtaga gtgtggcacg 360 actcggactc gggcgatgcg tattacggat ttaatgaggt ctcattagga taccataggc 420 ctagatgacc acgttacatg tacaggtaga ctctgataat tggggaggcg tttgctgcat 480 ccgcatccgc cgtgccacca aaaaaaaaaa aaaaaaa 517 // ID HO747686; SV 1; linear; mRNA; EST; FUN; 732 BP. XX AC HO747686; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_D03_025 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-732 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 9ff9fae9840c2bf7461284d6e6fb1d9b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..732 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 732 BP; 134 A; 270 C; 184 G; 144 T; 0 other; cttaacaaca acgttcgcga ccgtgcgacc caggctgtcc tcgctggccc ggccatggcg 60 ttcatcgata cccagaagca gctcctcagc caactcgtgc tcgccggctc cagcagcaac 120 aacagctcgg acgcgagcac taccaccatc tccccgtcgg aggccacctc catcctcgcg 180 agcgcgtctg ctgcgtctgc cactgccacc gcatccgcca ccgacagcgc gtcctcttcc 240 cagtcgactc agtcctcctc gtcatcgtct tcgtcgtcat cgtcctcctc ttcgtcgtct 300 tcgtctaact cgtcgaacac ccaggagtct gataacacga tcgtcgactc gcccggaacc 360 cgcaacctcg agaccggccc gagcaagggt ggcgcgatca ccatcgacgg atggcaggcg 420 gaccagcccc tcctgtccga ctcttcgtcc gcgtccgcgt caggcagcgc ctctgcaagt 480 gcaagcgcga ctgacagcgc ctccgccgct gccgcatcct cgaccggcgc cgccgcggcc 540 acgaatggtg ctgtgtctgc tccgtactaa attatccact tggtttaaga cgacacgcga 600 tgtgacatag ggtatgacgc gcggacgttt ttattcctct tgcacacgga ctcggcggac 660 actttgggcc tgatgaggac tattgctact ccatacatgc atcacagcta ttcgagcaca 720 ggctcgcgat ag 732 // ID HO747687; SV 1; linear; mRNA; EST; FUN; 338 BP. XX AC HO747687; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_D04_026 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-338 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; b570eea988d88c5f2aae91b535daa10c. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..338 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 338 BP; 69 A; 110 C; 103 G; 56 T; 0 other; gtgaggtgct tccgcgagac caacccaccc ggtgccggtg gacgactgct ccagatgtcc 60 tcgaacgggg gactcgttgg catgcccgcg tgctccttct attgcgcatc caagttcgcg 120 ctggaaggtg cctccgaaac gctcgctgcg gagctggacc ccgcatggaa cattaaagtt 180 accatcatcg agcccggatg gatccgcacc gaagtcgcgc cgaaggcgat ctggtcgccg 240 caacaccccg cataccagaa accgactctc cccactacgt acattcggaa tgccggatgg 300 gacaaggtca cggtgtggaa ggacgcgcgg cggagcgc 338 // ID HO747688; SV 1; linear; mRNA; EST; FUN; 744 BP. XX AC HO747688; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_D05_041 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-744 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 9a8aeccea338fe5ccbde5d0e57fdad97. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..744 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 744 BP; 163 A; 213 C; 236 G; 132 T; 0 other; gaggcaggcg acaaccctcc acaggagtca gggttggtga acaacggttg gtacggtaaa 60 ttccatacgg agatgttctt ctggcacagt gcgcactggg cgctctggaa caactgggac 120 ctcctcgatc gcgcgaccgg ctcgtacgcc cgcttcctcc ctacatccat ccaacgcgcc 180 cagatacaag aggattattc cgcaggcgca cggtggtcga agatgacaga cccatcaggt 240 cgttcggccc caggcgagat caacgagctc ttgatctggc agcagccgca cccgctcctg 300 tttgcagagt acgaatatcg tgcaacacgg tcgcgcgcga cgctggagaa gtggcgggac 360 gttgtgcggg cgaccgcgga ctggatggcc gtgtacgcca agcggaacga gaacaccggc 420 ttctatgact tagggccacc gatgtacgtc gtcagcgaag atacaagtcc gaacgcgacg 480 tacaaccccg cattcgagct tgcatactgg cgattcgggc tggatcatgc gagtacgtgg 540 atggagcgcc tcgaagaagt tccggaggca tggacagaag tgcgagataa cctggcgccg 600 cttccgatcg agaatgggtt gtatgcggtg tacgagggca tcccgagcga cttctgggac 660 acgccaacgt acacgaacga ccacccggcg ctggtggggt tgtacgggtg gctcccgcag 720 acggcgaacg taagccttga cttg 744 // ID HO747689; SV 1; linear; mRNA; EST; FUN; 242 BP. XX AC HO747689; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_D06_042 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-242 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 5d2415407c7adf7044ff46a48339125e. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..242 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 242 BP; 87 A; 55 C; 57 G; 43 T; 0 other; gtgaagtcat cctcgttcac atccctcggg tgcagtcggt ggcggcggca gcaggcccgg 60 gttcgtggta gggatgtgcg cttcgcgatg catgtcctac gtagatgccg taccgtcctg 120 tactctatcg cggacctcgc tcaggtcacg ctgcactcga tacattgcac ttctgggagc 180 cgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 240 aa 242 // ID HO747690; SV 1; linear; mRNA; EST; FUN; 751 BP. XX AC HO747690; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_D07_057 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-751 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 77721def76454fd1a3867dd284ea52d1. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..751 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 751 BP; 135 A; 231 C; 188 G; 197 T; 0 other; gagacaacgg ctcgcttgta acctttaagg cccctgacac tcagcccttc aacatcgcgc 60 tggtcgctga cgacacgaat gagacccctt cgttcctcgg cgcagttggt aacaccgcaa 120 tcgcaagtgg ttctgggagg tgcgtcactg gcatcgcttg cgaaggcttc gcgacttctc 180 tcgtgctcaa ttgccggatt ctaatgtttt cttcgtctct tctctgtgtc ggtagtgcgg 240 ccgcgctcag caatgtcacc caatgcgctg ttcttcgtga gcaactgatc acccggttag 300 agccccatat gcaacgaaaa cgcccagcgg acgagagtca gtgcgacact gactccgttt 360 gcgacgattc ggacgacgaa atacggaaca caaacgagac gacggccatg acgacgacgg 420 gctcgaagcc cgtctcgctc ctactctacc acccctattc gctttccccc ttgccctgct 480 ccctggtgcg tctgtcaccc gcccatccag gcgcgttttt cagttctacg aagaacagct 540 ggggcgagcg ggcgccggac tattttcccc tcaatacatt tacgtacctt atctattgac 600 ttttttctac tttacgctgc cagctagttg tacattcggt ttcttttggg ccttggtttt 660 ctgggttcaa cggacttgcc tcgcacggta cgaaccgttt tgatgttgcc gtgctccggc 720 ctacgtgtcg gtgcttggtg cttcgcgact g 751 // ID HO747691; SV 1; linear; mRNA; EST; FUN; 740 BP. XX AC HO747691; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_D08_058 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-740 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 0ed78438d470b772b23de0bf6cb4dd98. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..740 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 740 BP; 176 A; 208 C; 202 G; 154 T; 0 other; tatataccca gtagcaatcc cgcaagccaa tacacactcc tggagaagct gggaacgggc 60 agtttcggca ccgtctacaa agcgatccac aacgactcca aacagatagt ggcaatcaag 120 cagatcgact tggaggactc cgatgatgac atcactgaaa tccagcaaga gattgccagc 180 ctggcacagt gcgactccga gtatgtcaca agatactatg gctccttcgt cgtggcgtac 240 aagctgtgga ttgtgatgga gtacttggct ggcggttcat gtctcgatct cctaaaggcg 300 ggtgtattct cggaagccca tatcgccgtc atcatgcgtg agctcctcct cggcctcgat 360 taccttcaca acgaagggac gatacaccgc gacatcaaag ccgcaaatgt cctgctatcg 420 gcgtcaggga aggtcaagct tgcagacttc ggcgtggccg cacagctcac caacacgttg 480 cggcatacat tcgttggtac gcccttctgg atggccccag aagttatccg tcaggcgggc 540 tacgacgcga aggcggatgt gtggagtctt ggtatcaccg cgatagagat ggccaagggg 600 gaaccaccat tggcggaata tcacccaatg cgggtgctct tcctgattcc gaaggcgaag 660 ccacccgtgc tcgagggccc gttctcgctg gcttttaaag acttcgtggc tcagtgcctg 720 acaaaggatc cacactcgcg 740 // ID HO747692; SV 1; linear; mRNA; EST; FUN; 770 BP. XX AC HO747692; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_D09_073 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-770 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 077cac3251accdcce89de72ce2ca8441. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..770 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 770 BP; 166 A; 250 C; 187 G; 167 T; 0 other; ccccacactc gtttaacgtc aactatgaag aacgggttca aaacgattcg tttgggtcac 60 gccgttggtg cgcatcaaca tagcaccccg catagtaaac gtgatggctg ttcaaccggc 120 ggtctctaca agacacccac caagggccag acagtcgatg ccagtcagcc agtcgctatc 180 agctgggata cttcatgcct caacacgaca gccgtcgaca tttacctgta tgccccaagc 240 gctgcccgcc cggttatcca cgagtgggag aatgtcaact acaagactgg cagctacaat 300 gcaaccctca aacctggctg gtggaactcg acctcgtccg tgaacctcca attcaccatc 360 gttcctgcgg ggcaaccccc ctttgccgct accctccctc ctggcccatt cttcaccgcg 420 acgcactctg gtacttcaac tgacggcacg gccgtcgctg gtgccgtcga acaggtcaac 480 aacacgccca ctcaaaagca cggactgtcc aagggtgctg tcgccgctgc cgtcctcatt 540 ccccttctct tggtcattgg gcttatttcc gcggccttcg tcaggatcag tcgtcagaag 600 ggcaaggaga agcgcaagca gttcagcgag atggttgata agcgtatgtc gacaatttcc 660 actgactgga agtcgctctc cggcgccgga gccaatgctg ctgtccgaaa cagcatggct 720 atgtcaggca atcgtgcatc gtcgttctct ttcggtgcta tccgtccctc 770 // ID HO747693; SV 1; linear; mRNA; EST; FUN; 283 BP. XX AC HO747693; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_D10_074 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-283 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 5ddae62ffb3a39414e20efca3c34e2a1. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..283 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 283 BP; 88 A; 75 C; 74 G; 46 T; 0 other; aacgttttca cgtacaacaa gtacacccaa atcgccgccc gcgccctccg cgcgtcgctc 60 aaggaggagg agcgtgtcgc cgctgagaag cgcggcttga cgattctccg gtaccaacgc 120 tgggagaatg gccagggtgg cacgcagacg ttcttgaacc cacctcccga gacggacgcg 180 gccaagaagg ccgcggtcta gagttttatc tgtgtattcg tttagcagca ggaatagacg 240 tttccaaagc cgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa 283 // ID HO747694; SV 1; linear; mRNA; EST; FUN; 646 BP. XX AC HO747694; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_D12_090 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-646 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; efe1a0122406218ce140239883775536. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..646 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 646 BP; 124 A; 173 C; 185 G; 164 T; 0 other; tttttttttt ttttttttat gatatacagt gattcattca ctctacgctg actgaaccgg 60 gtcgtctagc ggggccgcga ccgcgaccgc atttagtcta ccttggtgct ctcgacgttc 120 cagtactcct gcgcgtagtc ttgaattgca cgatccgaac tgaacttgcc catcttcgca 180 gtagtccgaa tactcttctt gatccattcc gagcggtcct ggtaagcctc gtcgacgagc 240 ttcaaggctt ggatgtagga gtcgaagtct tcggtcaaca agtagtagtc gttttggcgg 300 atcgtgttga gaagcggttc gtacgcgcca tcgtcgccga agcgaccgga cgccacctcg 360 ttcaagacgc gcgcgagcgg cgggcacttc tgctcaatcg gcactgggtg atacatgtgc 420 tggtagcgga gatcctcaac ggcaggcgtc aagtggccga agaagaagac gttgctctcg 480 ccgacctcct ctgcaatctc aatgttcgca ccgtcgacgg tgccgaggag caggccgccg 540 ttgagacaga acttcatgtt tgacgttcct gatgcttctg tcccggctgt cgagatgtgc 600 tggctgatat cggacgcggg gatgaggatc tcagcgagag acacgg 646 // ID HO747695; SV 1; linear; mRNA; EST; FUN; 812 BP. XX AC HO747695; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_E01_007 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-812 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 785d4badec06ba8a588db7e52dd9b004. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..812 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 812 BP; 152 A; 301 C; 214 G; 145 T; 0 other; gttacaacct ttgcaactcc acgaccgagg gcgtcaactc gctctgccag accgccttcg 60 tcaaccacct cgacgactgg tgtgtctggg cgccccccaa gcccggctcc accatcggag 120 acaccgaggg tgaggaggtt gcttggtgca ccaagaaggg ccgtggtaca cgtatcatcc 180 ccgctggcgc catcaccggt gtccagttca tgaagacccc tggctacctc cagatcgtcg 240 gcttcatcga ccagaccaag atcaacctcc agtctgatga tggcggagga gagctcgacc 300 cccacggtgc tgatctccgt ggtaaccctc ttggcggtct cgtctactcg aacgggttcg 360 cgagcaacaa cgggaacaac aacacgttcc agcaggtgat cgagtggcac aacttcatgg 420 gctcgaactc gttctgcttc aaggtctgcg accccgctgg ccccaacgca ccccagctct 480 gccagcacat cttcgatcgt attggtgtcg catacaactg ccccaacgcc gcccagaacg 540 gcacgttcga gtactgcgag ggtgagaacc aggacccccc tggtgtctac acctcgaacg 600 gccaggtcgt gacctacacc cagccccctg agtcgctcgg cccgatctcg acgatgccct 660 acgacccaaa gatccccgcg tcctcgaact gcgtccagct ccagtccgcc gccctcttca 720 ccgacctccc cgcccccacc ggcgcgtcga ccgctgctag cggttcggcg actgccaccg 780 gctcgcgcgc tggctctgct ggtgctagcg gc 812 // ID HO747696; SV 1; linear; mRNA; EST; FUN; 804 BP. XX AC HO747696; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_E02_008 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-804 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 0330958365188476e0dbbe4bef19c222. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..804 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 804 BP; 188 A; 254 C; 227 G; 135 T; 0 other; gactcattcg tggcaaacac aggatggctg atgccccgtt gccagctttc aaccgcgaga 60 cgcactcgaa atacacggaa agcccaaatc cgacgttcag ctacggacag aaagtcgaag 120 ccactgaaga gggcaagaag tgggtcgacg gcgagaaggc cgggtggacc gtcgtcgatc 180 cgtccaagga ggaatcaaga aggatttacg cgctcatgat cagcgggatc atcccccgtc 240 ccatcgcctt cgtatcctcg gtctcagaaa ccggcgttga gaacctcgct cccttcagtt 300 ggttcaacca ggtgacgcag tcgccgccgc tcgtctccgt gtccatctcg cagctatccg 360 acggcggcct gaaggacacg gcggacaaca tcaagaagac gaagcagttc accgtgaaca 420 tcatcagcga gccgtttgtt gagaacgcaa atgtcacctc gctcgactcc ccgcgcgagg 480 tcagcgagtg gggcatctcc ggcctcacga aagcacccag cattcacgtc aaggcacctc 540 gggtcaaaga gagcgcattc agcatggaat gcgaactttt taagaccgtg gacatcatcc 600 accctgagac cggcaagcag acggccacgt tagtcctcgg cctcgtcaag tacttccaca 660 tccggaacga cgtcctgaac gagcgcggcc tcatcgactt catgaagctc aagcccgtcg 720 gccgtatcgg cgacatctcg tacacgcgcg tgggcgacgg gttccgcctc gcgcgcccga 780 gctgggccgc agagggcgcg aaga 804 // ID HO747697; SV 1; linear; mRNA; EST; FUN; 610 BP. XX AC HO747697; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_E03_023 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-610 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; d22b372cad77c70086d73a451fd3dc01. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..610 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 610 BP; 120 A; 166 C; 176 G; 148 T; 0 other; ggttgacgtt aagcctcggt ttctacctcg tatggctctc cgagtattgc ctcacggtcg 60 agattatccc cccgtcagct gggtccgagt cgccaacgac gccgtacgaa cccctggtat 120 ctcttccccg cgccatcggc gtgcacagcg cgaacaatga cgaggatgtt ttcagtgata 180 cagggattcg ggagatggtg gaaggcgaag cggacgatcc ggacgccgca tacactggtg 240 tagctgcacc cgtcgtgtta cccttactgg atgcgcctgt cgaggggtcc gaggtggagg 300 gagctggtgc ctgtgacgac gctgagtagc cggcacgccg cttgcggaca gggtggaggc 360 ttggtttctg actcgcacca tctttttatt catggccatt gtctcgacga tggattacct 420 atcggatgtg ccgaaccagt tcatgtggca gaagaggacc aggagggctt ggcacgcgca 480 tcccggttcc gagtccgtac tactcttgcg ctcactctgt tgtacaaatc cctgctattg 540 tgccggatcg attgggactt ctatactcat ctgtacagca caattaattc taaatcgaat 600 tgagaaccat 610 // ID HO747698; SV 1; linear; mRNA; EST; FUN; 784 BP. XX AC HO747698; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_E04_024 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-784 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; e6f6b67ea6d7b8629e6e8d337f90fd38. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..784 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 784 BP; 159 A; 277 C; 204 G; 144 T; 0 other; ctcttgtctc tgtctgctcg agctgtcgct cgctctcctt caggccggtg ccaactgtac 60 acactaacgc gacccggggt cgtttcgtct tcttcacgct cacaaggcac cctttcactc 120 cttcgaatcc accggtcggt gtcgttaagt aatttcaagc accatgtacg ccaaggctgt 180 cctcgtcgcc ctcctttccc tctccctctc tgcctccgcg gcgaagaaca acaaggctgc 240 tgacacatgc gacgccgctt cagccaagac agtaaccgtc actcagaccg ttgctgctgc 300 ctcaactggt gtcctcgcag ccaacccagg tgcgaacagg ggcacgggca gaggtactgg 360 caggactggt actggtaaga ctggtaccgg cgccaacaag ggaggaaaca atgctggggc 420 tggtaaggct gccacgacca ccacttctgc tgccgctgca gccacgacca ccgctgctgc 480 gacgacgggc aagacgacgg gcaagggtgg caacaacgcc gccaccggcg caagcgccaa 540 tgcccagacg tcgctgaccc ttgatccctc caacgtcttc accgctttcg cttcggacgg 600 caatgcgaac gcgaccgctg gccaggagcc ctcgatcacg tcgacgaaca acttcatcaa 660 cttctgcctc gagttccccg gcgtgcagaa gaccaacggc cagcaggtca tcgagggctc 720 gtgcaaccag accccaatgg gcctgatcgc cgcccagtcc aagatgccct cgtccaagtt 780 ccag 784 // ID HO747699; SV 1; linear; mRNA; EST; FUN; 525 BP. XX AC HO747699; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_E05_039 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-525 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; f9780900da7641ae805df8eb76780313. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..525 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 525 BP; 90 A; 214 C; 152 G; 69 T; 0 other; ggcccatgca gcacccgccc aacgttccgg gtggtctcgc gatcggcacc gcccccagct 60 ccaaggagga tgacgcgcaa gaggagagtg aggatgctcc cgcaggcgac gctggtcgca 120 accctcgtaa gcgccgtcgc gcgtcctcgt ccgtaaacca accccgcggt aacaattctc 180 cggccgtgcc cggttcgtct ggcgcgcccc aggctggcgc acctagccag tcaccgcacg 240 ctcgtccagg tcaaatccct cccggtgctc ccccaatcac catccccggc gtcgcgcccc 300 agatgcccct cgaatcctcg ccgcgtcaag gcgcccaggc ggcgccgcca cccacaagtc 360 ccaactacgc ccaaccggcc ggtggctacc cgcctgcgcc tgccagcggg gacccaaaca 420 tgtacgcgca cagcggctac cctggcgcgc ctccgcctca gcaatacgcg gcgttcgggc 480 acgaaggtgg cgcgcaatcg gcggtcaacc agaatccgtg ggcgt 525 // ID HO747700; SV 1; linear; mRNA; EST; FUN; 479 BP. XX AC HO747700; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_E08_056 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-479 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 95a29971a2d707e50dd6e1fef447c6eb. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..479 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 479 BP; 64 A; 208 C; 126 G; 81 T; 0 other; ggtctcgctc acgtccccgc cctcccgcct tgacctttac gacctcctgt ccttcacgtt 60 cttatattcc gtcatgaagt tcaacgcctt cgccctcacc gtcgtcgccg ccgtggcgag 120 cgtccacgct cagtccagta ctagcggcag tgccgccgca cccacctcca ccactggtct 180 gtcgccctgc atcctccagt gcactacgca ggctgccacc gcggccggct gcaacggcgt 240 caccgacctc acctgcgtct gcaccaacgc caccttccag cagcaagccc gcacctgcct 300 ccagcagtcg tgcacggccg cagaggtcca ggccgcggag gccctccagt cccaggagtg 360 tggtgccatc gcatcctcag gcgcgagcag cgcgacctcc gcggcctcct ccgggaccgg 420 ggccgcatcg tcctcgggca cgcgctccgg cgccgcctcg tcctcgggca ccgcgtccc 479 // ID HO747701; SV 1; linear; mRNA; EST; FUN; 790 BP. XX AC HO747701; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_E09_071 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-790 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 80b9d0e120c71baa456538ceeedf6b1a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..790 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 790 BP; 166 A; 257 C; 241 G; 126 T; 0 other; cttgccccaa ccatcaagct caacaacggc atcgagatcc ccgtaatcgg cctcggtacc 60 tggcagtcga agccggaaga ggtcgtgaat gccgtagagt acgctatcaa ggaggcgggt 120 taccgtcaca tcgactgtgc atggggatac ggcaacgaaa aacaggttgg ggaaggtatc 180 cggaaatctg gtgtccctcg ctcggagatt ttcgttacta gcaagctctg ggcaacccgc 240 ttgcataacg tcgaagaggc gctcgatgag tccctcaaca acctcggcct cgactacctc 300 gatctgtacc tcgtccactg gccgatccac ctcaacccga acggcaacca ccccagcttc 360 ccgacgctcc cgaacgggca ccgcgacgtc gtgcgcgact ggccgctccg cgagacgtgg 420 aagcagatgg aggcgatggt gaagaagggc aaggtgcgcg cgatcggcgt ctcgaactgc 480 tccgagcgca tcctcgagac cgagatcctg ccgtacgccg agatcccgcc cgcggtggac 540 caggtcgagc tccacctcta caacccgcag cacaagttca tcgcgtacct caagagcaag 600 ggcatcgtgc cccaggcgta ctcccccctc ggctccacag gcagcccgct cctcaaggac 660 gacgtcgcgg cggaggtcgc gggcaagcac gggttggcgg tcgcggacgt gctgctcggg 720 tggcaggtcg cgaacgggat cgtgacgctg ccgaagtcgg tcactcctgc gaggatcaag 780 gggaacatcg 790 // ID HO747702; SV 1; linear; mRNA; EST; FUN; 629 BP. XX AC HO747702; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_E10_072 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-629 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; f863214f431fada5aa2dcdb761465913. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..629 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 629 BP; 93 A; 240 C; 97 G; 199 T; 0 other; tctctctctc tctctctctc tctctctctc tctctctctc tctctctctc tctctctctc 60 tctctctctc tccttgtgtc ctcgcgtctc ccgtggtcac ggtcacatcg tgtgctgctg 120 ctgcttcgcg atccgacttt aaatttcccc tctcgacata tcgcttcttc tgcttcctcg 180 tcgtcaccct gctcgcctct cgatcgctcg gctccgttcc ttcttcctcc atatacctaa 240 tgtcgtagta tacacgtacc gacccgtagc cactccctct ctcctctccg ctctccgcct 300 tccgttattc gttattcgtt atccgctgtc tcacaccgtt atcgcctgcc cggctgtccc 360 tctcgtaacc tagcttccct gcctcccggc tcccaagttt cctctcatgt cccattctta 420 gcttttcgcg cgcgagcacg ttaccctgct gttgctgtat tcccccaaac cccttgtgta 480 cttgtacgtg tacccgcctg ccgccgtcgc cgttatccat agtataccta ccgccagggc 540 ccaatcccgc cgcataccgc ttcgtaccgt atgtcctccc gaataagcta acgttatgga 600 ttagatgtca aaaaaaaaaa aaaaaaaaa 629 // ID HO747703; SV 1; linear; mRNA; EST; FUN; 732 BP. XX AC HO747703; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_E11_087 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-732 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; bf32b76176fa7eca74c9f23458105542. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..732 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 732 BP; 145 A; 228 C; 216 G; 143 T; 0 other; gggatgacgg cgactacaag tatgcgaagt acaagccatc ctggcctgac gtcaagtggg 60 agcctctgaa ggagttcccg gtgacggaca gcgcgcataa tgcggatccc gagaagaaga 120 gcctgttgtc ggccgcgtca aaggtgacgc acataacgcc cgtcatcggc accgagctgc 180 tgggcataga cctgcgacag ttgtccaaga gccagaagga cgagcttgcc ctgctcgttg 240 ctgagcgcgg tgttgtcttc ttccgtgacc aggaactcag catccacgag cagctcgagc 300 ttgctcggca tttcgggcct ctccacaagc acgccacgac gccgattccc cgggagcccg 360 gcctggagga agttcacgtc gtatacaccg atggttctcg ccggccagac ccttccgcgt 420 tctcgaagct cgatctctgg cactctgatg tgtcctatga gctccagccc ccaagcacca 480 cgtccttcaa ggtaatcacc agcccgccgt atgggggcga cacgctttgg agctctgggt 540 atgccctgta ttcttccttc agccccggct tccaaaaata cttggaaggc ctatcggcgg 600 tgcatagcgc ggttgcgcaa gcagatggcg cccgtgctgc tggacagacg gtgcgacgtg 660 agcccatcga gacggtccat cctgtcgtgc gtgtgcaccc tgtgacaggc tggaagagcg 720 tctacgtcaa cc 732 // ID HO747704; SV 1; linear; mRNA; EST; FUN; 793 BP. XX AC HO747704; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_E12_088 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-793 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 37c29459787b0d5d51154f15b89a0ecb. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..793 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 793 BP; 159 A; 254 C; 219 G; 161 T; 0 other; cgcaataaca caaatggcgg ctcggaagaa gcatgacctg cgcaatcctc tcggcgaaat 60 tctacctcgc ccggcggtgg cggcggcgac atttcttcgc tcatccgtcg tgggacccct 120 tccgtatccc gtccgagctg cgcggtgctg ctcctgcaac cgcgccgccc aggaaagaac 180 cacaacgcgc gccccagtgc ggtgccccac tgctgccaag gctctacata cttcgaagat 240 gtgttccacc ctctgatggt tgttgtctac cttcacttag gcggccgcgc ctcaagctcg 300 ccgagctcgg catccaacat tcttcctgcg cgcagacgag gagcggggac gggaatacga 360 tcctcctgta taacggggtc gcgcgtcgac acggcgacgt ccttgcgagt tccgggtctc 420 gtctagtcgt tgcccagtcg acatccaatc cggccagacg tgcgacgcgc tactcaatcg 480 cagctacgta tgttcgagag ccgcgccacc gaccatacgc catgccggtt gtcgatcgac 540 caacgatcgc aagcgttgca tttgggtcca ccatcataca caagttaaac gccggggacc 600 gaatcccgag gggcgagcga tttgtttgac caagccttga ctcatcatgc atcacgccgt 660 ccgccccgtc tctgaatgtg tcaaatctca gtagtcgtct cagctcggcg aatcggtggg 720 gggagggaat aagtagcgtt gtgcagatgg ttcagtactc acagggcacc agtgacgaac 780 gttaacgtta cct 793 // ID HO747705; SV 1; linear; mRNA; EST; FUN; 587 BP. XX AC HO747705; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_F01_005 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-587 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 98602691934a17ccb12219ba299c0f0b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..587 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 587 BP; 121 A; 205 C; 176 G; 85 T; 0 other; ttcgtctgcc gcgacgaaca aggtctcctt cacaatccgc cgaccgaccc ctgcctcgcg 60 cgcggactca gactcagact cctcttcttt caaagtcccc gcgctccccc gccgcatctc 120 tggcgccagc acacccaaca gccctctcgc gcggaacagc cctcccccga ctcgcacata 180 cagcgagcgt gacagctcag atgaggactc tggagtggag gacgagctcg tgacggcgtt 240 cgatgagttc ggcgcgaagc gactccatga gaggccgaag ccccagggcc cccttgtcat 300 tcctgcgctg cagaacagag actggcgcga gctcgcgcgc aagcgcaagc agatgtacgt 360 tcccccgagc gctgcagcga ctactggcgc agacggatcg gtgggcggcc tcggcacccg 420 cgacaccatc aattcgggtc cgcaggtgtc aggcctgcag atgcggaaac gtgtaaagct 480 cgaggacacg caggacgagc ctatgccgga cgacggtgtg gagcagccta agcccgaaat 540 gaaggaggag gaaacggacg accagcgggc cctccgtgcc atcctcg 587 // ID HO747706; SV 1; linear; mRNA; EST; FUN; 484 BP. XX AC HO747706; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_F03_021 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-484 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 7eee1376af1b28cf9c92aa3f2469dc1e. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..484 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 484 BP; 136 A; 122 C; 125 G; 101 T; 0 other; aagaagtgtc tcaacgaggt cctgaagtat ggtggcccct tcagccctcg cgacctctac 60 ccatatcagc ttgcgctgca ccagatagac tcgatgcgta aggagggcaa gtttgtcggc 120 attgatggga ccatccctga aggtcagggt atcgtcatgg cccacctcaa cgagtgccac 180 gagctcctcg agatgctgaa agagtcgatg gacgacgcgg aggaggacga ctacgccgac 240 ggcgacgaat atgacttcga cgacccatcg tcagaagagg aacagtgacg acgctcgatt 300 tggggacttc cgagccgatt gactgaggaa ctgtaatgga cagtgcaacc gccgtgtgtc 360 atagcagatt ttcccatttg tacgagtgca ccattatact agaggttttg gacggaacaa 420 tacgttcctc tccacactcg tctatcatca tagcttatat ccgaaaaaaa aaaaaaaaaa 480 aaaa 484 // ID HO747707; SV 1; linear; mRNA; EST; FUN; 716 BP. XX AC HO747707; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_F04_022 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-716 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; e2324457ca0722585fd0159ba22aa5ab. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..716 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 716 BP; 155 A; 188 C; 198 G; 175 T; 0 other; tttttttttt tttttttttt cgagttggct caaacacggc gggtttattg gacaacgcta 60 acgcacttga cattcacaac gacaaagcaa caacaactct acgacattcg ggacactcag 120 atacatcgcc atcctggcta ctggcaagcc acagttctac gggaagtgtt tgatagctgt 180 tcaatggtta acaagaacga gagcttcaag cggtcttcca caggaggtac aaatagctct 240 tgccgcggcc tttgttgatg tcgatagtgt ttccagccca ccccgcagga cgctcggtca 300 tcgcattgtc aagacgcagc agagcaacct cagtaaccct ctccgtctgc gtggcgctct 360 tctcgagaag gaggtagcgg tagtcgccac ccgcaccctt ggcgaggtcg ctcagcgagc 420 cgtcggcgct cccttggatg gcgatgtcga aaccagtgac cgcggcgtcg cggtcggtcg 480 tgaagacagg gacgagccag acgtattttc cgccaaactg gtagttgatg tcgtcgttct 540 ggtggttggt ctcgaggacg cggccagagg gagcttggga gggctcgtcg ccgtagacga 600 cctcgatgtt ggagataaac tgcatgattc gtgtggcgat gtactgacct ccatgttatg 660 atcttgctgg gatcttgcac cagcaacatc agttctcggg gcagctaaac acttta 716 // ID HO747708; SV 1; linear; mRNA; EST; FUN; 666 BP. XX AC HO747708; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_F06_038 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-666 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 0f586511d8e66af2d6ad28af9ff6722b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..666 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 666 BP; 106 A; 228 C; 236 G; 96 T; 0 other; ggcattgtcc tcttcagcgg tgcgctcgag gacacgttcc ggataccatc atcttcatcc 60 gacacgccag ccacctggcg ggcgtctcgc cggacacgtc gcgcggccgg cagcagagac 120 agagacgatc tgtctacgga cgacgaccca gacagcatcg tggtcatccc gcccctgggc 180 gaccgcgagg tcgagaacga cgtccggcgg ctgctcggcc accatggcgt gcgctcgcgc 240 cagagcctcc tggaccctga gacggacccg cgcgagccgc tcgacagcgc cggggagagc 300 gcacgggacc gcgatcgcga tggcgacgag atggacgtgg acgagctcga gagcgactgc 360 gtgtcctccc atgcaccttc gcgttccggt tctccaaccc cagcgtcgca gccgtccgca 420 ggtccacaat ccgtcgtggc acgcccgctc gagctcctgc gctcccgccc gacgctgctg 480 gcgaggcgcg agagctcggg gccgtatggg tcgcgttggg gcgtgtcgtc gtccgcgcgc 540 aggcatcgga ggggcgccgg acagggtcgc gatcagcgag aggagggtga ggaggagcag 600 gacctcgcgg ggatgtgctt cgacccgacc ggcacgttcg tgtatgtcgc gagcgtcaag 660 ggcatc 666 // ID HO747709; SV 1; linear; mRNA; EST; FUN; 639 BP. XX AC HO747709; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_F07_053 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-639 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; d0e7cd16c3ba7e272171e99e4ee3b7a3. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..639 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 639 BP; 144 A; 176 C; 163 G; 156 T; 0 other; cggctgtcga gtcccgctcc gctcaggata tcttcacgcc cccggtattg aaccccacca 60 ctggtacggt gtggaccgtc gggtctactc agaaggttac ctgggacatc tcgaacccgc 120 cgcagaccat cacgggcagg aacgaatcct tgatcatcct ccggaagggc ggcattacta 180 ccccagtcat cctccagaac aaattcgaca tcctccttgg gtccattgag gtcaccgttc 240 cgtgggtcgt ggatggcgac gattactccc tcatcctgtt cggcgactcg ggcaacgaca 300 gcaaggactt caccattact ggcagcggca tctcgttcta agcatctcgc tcgccagctg 360 aagaatggtg cttcgacgtc attagactcg tgacgaaccc cgaatgattt ggatggatgt 420 acgacggact tctttgttgg ggaatggact taaagtggac cgctcgtacg tacgtcgggc 480 gcattgcgtt gtactcgcgt tcacgttcgg tgtagtacta ctgctcctgt tctgctgtac 540 ggcgttggtt tcaatggttc attttcgcaa ttttcggaca gcaggacatt gcctgtgtcg 600 tctttgagcc gtcaaaaaaa aaaaaaaaaa aaaaaaaaa 639 // ID HO747710; SV 1; linear; mRNA; EST; FUN; 754 BP. XX AC HO747710; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_F09_069 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-754 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; a601b1f3c0c10223f989f17050803b5c. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..754 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 754 BP; 148 A; 250 C; 176 G; 180 T; 0 other; gacatttcct ccccatcgct tccgtcttcc tctctctttc tccccacacc accccctata 60 cgacccacca ccatgtctga cgacgagcag cacaaccaca acttcgagca ggccaatgct 120 ggggcttcca ccaccttccc catgcagtgc tcggcccttc gcaagaacgg ccacgtcgtc 180 atcaagggcc gtccctgcaa gatcgtcgac atgtccacct ccaagacggg caagcacggt 240 catgctaagg tccacttggt tggcatcgat atcttcaccg gaaaaaaact ggaggatatc 300 tccccctcca cccacaacat ggatgttccg aacgtcatcc gtaacgagta ccagctcgtc 360 aacatcgacg acggtttcct gaacctcatg actcaggatg gtactcccaa ggatgatgtt 420 aagctgcccg agggcgatct cggcaaggat gtccagagcg gcttcgatga cggcaaggat 480 ctcctggtca ccatcgtctc tgctatgggt gaggagcagg ccatctcctt caaggaggcc 540 cccaagggtg cgtaggcggt gccctctggc agtggccatc gttttcctcg gtctctcgtc 600 gtcgtgtttg tgtcgttcac acgcttaccg ttgtgttcca gttgttaagc atcagtatcc 660 cgtagcagta gcctcatcac tgtgcatgtg taccactccc tttgcccttt gcgcgccctt 720 gtattgctcc ccgccctgtg ttgtgtcatg caat 754 // ID HO747711; SV 1; linear; mRNA; EST; FUN; 631 BP. XX AC HO747711; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_F10_070 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-631 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 27e29946de8827a918c1192ba345cd4c. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..631 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 631 BP; 143 A; 167 C; 191 G; 130 T; 0 other; ggaacgatgg gagtgaccgc gttgtgcttc tggtcaacaa cctcggcggt gttagcgaac 60 tcgagctcgc aaacattgct ggcgaggcct gcaaagcgct ggtggccaag ggcgtgaagg 120 ttgaacgctt gatctgcgga acgttcatga cgagtttgaa catgcccggg ttctccatta 180 ctacgctcct actcccttct gcgtccgaga ccggcaagga agttccggac gcctccctgc 240 tcctgtctct cgtggatgcc cctgccaatg tccccggctg gaagtgggcc tccaccgccg 300 taccccctga caccgaccaa attccttccg gcgcgaaaaa gcagggttcc gtggcagctg 360 cacaggaaac cgtcaccttg cgcgccgaag atcagaaggc tgggcgcagc gcttacgtcg 420 aaggcaacag actcaaggag gagcgtgtgc ccgacccggg cgcgtggggc gtgaaggttc 480 ttctggagag tctgctgcag tgagtaggat gcaaggagtc ggtggtaccg aagggtttat 540 gtcaaggatg agtaacgaag tgctgtgtat gagtaactgt gtgtcgatca aacgtccact 600 tgttgttttt ccaaaaaaaa aaaaaaaaaa a 631 // ID HO747712; SV 1; linear; mRNA; EST; FUN; 513 BP. XX AC HO747712; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_F12_086 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-513 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 91710473d98cf0d4565f35633d4c70e5. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..513 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 513 BP; 93 A; 127 C; 154 G; 139 T; 0 other; tttttttttt tttttttttt ttttttaaac tttccaaaat atacattgtg catcacatag 60 atacgtacat acatacatct gcttcaagct actcgacgct cgtcgtttag tagttgtcga 120 cggtaacaac ctcctcgaac ttctcgctgg agtggtgctt ggccttttgc gcgtcgatgt 180 agtcgaggcc cttggtctcg acgatgcggt cgatgaacgc accggcgaag cccgcgagga 240 tctccttggc cttggcgtgg ctgtcgggct ggccgttctt cgcgacgtgg tcctcgtagg 300 ccttggcagc ctggcaaaag acaggtcagg ctagggccgc cacgacggag gatcttgcaa 360 cgtcacctcg taggatgcag cggcggcaat gagctcgtgg gagagcttag cctggtgggg 420 ggcgttgttg acctggtcaa aggcctgggc ctcgtcagac tcagagtgga agaaacccat 480 tgtgtcgtaa gcggtggcgg tggatgcggt ctc 513 // ID HO747713; SV 1; linear; mRNA; EST; FUN; 747 BP. XX AC HO747713; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_G02_004 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-747 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 1208caef0a1cad84c015d3398fdbb205. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..747 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 747 BP; 162 A; 200 C; 210 G; 175 T; 0 other; tttttttttt ttttttttgg ttgttccaag cagaatcccg gcaattggac atgaatttcg 60 taagcgttgg atggacaatg aacggacaag agaggtagag gtagaggtat gcagactagg 120 ggtaacttcg gtagtattgc caaccggggg atgggagagt gatggtgcaa aggggaggga 180 gtgtgcgcgc atcgtcatcg gacgcaaaga tgacgacgac agctaaagcg gcgagaggac 240 attcggaaaa cgtgaacgtg cgaagaacac tgaaagcgac caatataaag tgtatacgat 300 gtagaccttt tcgggtgatc atagcattgg cggaagcggg gtacaactgg gcgcgacacg 360 cttattcctc gtcgacgggg acctcctcct cgtactcgcc ctcctcctcg acggtagcat 420 cctggtattg ctggtactcc gcgacgagat cctgcatgtt gctctcggcc tcggtgaact 480 ccatctcgtc catgccctct tgggtgtacc agtgcaggaa cgccttgcgc ttgaacatag 540 ccgtgaactg gtcgctcaca cgcttgaaca gctcctggat cgccgtcgag ttccccagga 600 acgtcaccgc catcttcagg ccccgcggcg cgatgtcaca ctgcgccgtc agcacgttgt 660 tcgggatcca ctccacgaag tacgcagagt tcttgttctg cacgttctgc atctgctcct 720 cgacctcctt catcgacacc ttcccgc 747 // ID HO747714; SV 1; linear; mRNA; EST; FUN; 724 BP. XX AC HO747714; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_G03_019 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-724 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; adc7c8c37a3fcf9c2181b5b78feacb06. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..724 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 724 BP; 195 A; 204 C; 222 G; 103 T; 0 other; tcctgccagg tccttgagcc tcctcacctt tccaaccttc tcgccacctt ccctaacccc 60 tcatctcaac gttcacacct ccaaaacact cgaagttctt caatggcaga gatcaccgag 120 caagtcgacg agcagcagca gccccagacc tcggaggcca cgggcgaaca gaccacggcc 180 tcgaacttgt ccgcgggcgg cgacgtaggg cagaagttca acgtcgcgaa ggcgaagaag 240 gaggcgggcg atgaggcatt caagaagaac gacctcgatg gcgcccttag gaattaccac 300 gaggcacttc tgtacttgaa aggcctcgac aagaacgcga cgcagagggc actaggccag 360 cccgtgccgc agccgccacc gattgaagcc gtgaaccagg ctgccgagga aaagcagcgg 420 acagaggtgg acttcttgtc ggagaagatc tactcgaaca tgtcgcaggt gcacctgaag 480 aaggggaact ggaagcgggc gatcgagacg gcggacgtgg cgctgcagca gaacaagaag 540 aactccaagg cggcgttccg caaggcgcgc gcgctgaagg agcagggcta ctttgagaag 600 gcggagaaga tcctgcagga tctcattaac gaagctgacg aaagtgataa gccaacgtac 660 gaggaggaac ttggggacgt gcatgtgcga gagaaggccg cctcggacaa gcatgacaag 720 aaga 724 // ID HO747715; SV 1; linear; mRNA; EST; FUN; 692 BP. XX AC HO747715; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_G04_020 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-692 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; d878714e0df76a6b40fdd9de15e2ad3b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..692 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 692 BP; 143 A; 223 C; 186 G; 140 T; 0 other; tacttcctcc tcagcgcgtc ccccgtcgtc cccatcctct accatgtcga ccgcgtgcgc 60 gacggccgct cctacgtcac ccgcgccgtc cgtgcggtcc agcgggggcg caccatcttc 120 acaatgatgt gctcgttcca gaaaccggaa ccccggcagc ccactttcca gttccccatg 180 ccatctgacg tcccagcacc cgacgcctgc gacagcatcg actcggtcta caggaaggcc 240 ttggacgagg cgacggacgc gaaggtcaag gatttctggc gcgtcaacat tgaggagcgt 300 gcgcggagtc ctatcgacgt gaagctcgcg ggcgtgcggc accccaagga cgggatgccc 360 gtcttcatgt actggcacaa ggcgaggaac acgccgaaac gttatgatcc tgccttccag 420 aagtgcattc tgagctacat ctcagactcc cagttcattt cgggcatccg ccggacgatg 480 gccatccttc ataaagattg cgaagcagat ggcaacctga aggttgtcat gcagacgtct 540 cttgatcata atatcacctt ctacgatgac gacttcgact gtggagactg gatactctat 600 gtcgttatca gcgaacgagc cgccagtgga cgtggggcgg tgcatggtcg gatgtacacc 660 cccaacggct ctctcgttgc aatatgcaac ca 692 // ID HO747716; SV 1; linear; mRNA; EST; FUN; 726 BP. XX AC HO747716; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_G06_036 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-726 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 6c5d03c0e483441a29454a52be5fb5f1. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..726 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 726 BP; 145 A; 248 C; 189 G; 144 T; 0 other; tctcaccagc tgcatctcgc gccctcagtc tcaccacttg cctaccgctc catcctccat 60 cgctggcggc caccgcgtct gattcagctg ttccgcacct tcccagccct cgtcttctag 120 cttccatctg ccgatcgcaa gatgttcaaa aagttccaac cgtcctcgga cgtctcgggc 180 caagttagcg tgaagtcgtc ggtgcagagg tccatccgat ccaatatcct cgcgcaatgg 240 aagatcaacc ccgaaacatt cgagcagata tggcccaaaa aggagcccct cacgctcgta 300 aagtgcagag agcacatctc catctacacg ctgcatggag agccgctgtt cttccagcac 360 ttcgacggcc cgttcatccc cacgcttcga ctccttcaca aatacccctt cattctcccc 420 accgtcaagg tggaccgcgg ggcgatccgt ttcctcctcg ccggcgccca catgatgtgc 480 cctggattca cgtctgcggg cggttccctg cccccagctg agcaggccct ccccgccagc 540 acgcccgtcg ccatccacgc agagggcaag gagcacgcgg caggcatcgg catcaccaag 600 ctgggcacag aggagatcaa gtcggtgaac aagggcgtgg gagtggagac ggtaacgtac 660 cttggagatg atctttggag ggtgcagtcg ttgtagtggg accgtgtatc ggcaggcgtt 720 cgagca 726 // ID HO747717; SV 1; linear; mRNA; EST; FUN; 672 BP. XX AC HO747717; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_G07_051 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-672 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; df30c207b7922c599f6851c5745feb9b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..672 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 672 BP; 162 A; 172 C; 187 G; 151 T; 0 other; tttttttttt tttttttttc atttgggtac gcgaccacag attaccaagg tgttcgcagt 60 catgtggaat acactagcag aggtatggga attgaatatc gaacttcgca gacacgtatc 120 ggtccgacga gcatgtaacc tccggctcaa ttgcagcacg aatcgcgcgt ctcgggaccc 180 tctgcgtccg cgtgatatgt atcccattgg aaaatggtag cgccaaggaa ggcaccgatg 240 aagatgcaga tgataatgta cccgttgaag tacatagcaa gaagcatgac gaagtaaccg 300 accgcgaact gtaccatgaa caaagccgac cggaggagat gctgccataa cttgagctga 360 tcgatgtgcg agccctggga gttgagtccc ttgcgacttg cggtatcgct ttgctcctcg 420 gtacgaagct gaggagcgga cgcaccgttg acgcggtgca agtagcggtc gaactcgcgt 480 tggaagcggc ggagaccctc gagcgcgatg acgaggaaga tgaccccgat gcacgagccc 540 gcgaagccgc ctttactctg gacgtgccaa ctactcgaga tgaagcagct gttgacggtg 600 taccagttcc atagcataga tatcttacac gagctgcttc ccatgcccat gtccatgctc 660 atgcccgtgc tg 672 // ID HO747718; SV 1; linear; mRNA; EST; FUN; 727 BP. XX AC HO747718; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_G08_052 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-727 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 896cc620c4211a09f464b34b5c9d3fa3. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..727 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 727 BP; 168 A; 175 C; 216 G; 168 T; 0 other; gtttttttat cgtcgaccgt accaccatag actacggtca tagaggtaat gtatagggat 60 atatacaacg attggtggac atatttgtat gatctcgagc tcaaacaaag atacatggcc 120 cagactctat acacacgtca aggggtccgc ttagctctac gcaaggtgca aagttgttgt 180 acgaaggtca cgtcgccgct tgcactggga cttccgcaag tccggagggg ataccgacct 240 ccaccttcgg aataggggtg gaagtcccag atacgatgtc ttgggtagga acttggccaa 300 gcaagatgtg cagaattggg gcgaacgtcc tcaactggtt cggggcgtat cgctcgtatg 360 ccgttggttt ctggtacacg atctccgcaa cggcaatgag gatgtcagaa gtcaggcctt 420 tgatcgcgaa tgctctggac cggaagcggt gcatgcgata ggtgctgttc gctagccacg 480 gcaagtcgat gtccgacgta caatggagat cttcctcgta tatccaatcc ttgctaccct 540 ttggagcgag atcctccttc gcggcgaggg ctgggtccag gatggcctcg agggggtggt 600 caaatatcct gtctacttcg ccgggcgagg gcaccagtgt ctcaaggacc gacaggtccg 660 taagaagcgc gactactggg gtgacaagga gcttgctaga ggcgatatat ggtcgcagag 720 tgcatac 727 // ID HO747719; SV 1; linear; mRNA; EST; FUN; 740 BP. XX AC HO747719; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_G09_067 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-740 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 29ab9ed68e4f37b4b58afd753e13adac. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..740 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 740 BP; 137 A; 217 C; 242 G; 144 T; 0 other; agggatcgtc tggccgcgca ccggcttacc cttcgcgcaa cgccgccgtc cagttttggc 60 acgctctcgc ggacgctggt ggggttcacc atcagcgccg aggtgaactc gactcggact 120 tcaaggcctt ctgtagctac tacccactca accccggact cccgtgatgg ctcggattct 180 gcactgggcc gatgatccca ctacacgacg ttgcatcctg gatctacgac gcggtcttgc 240 ggtacggtgg gcacaaagat gagcatgcta acccggagct ggacggcgct cggatgctca 300 cgtacatctg agcttcaggt ctgcaggcgc gggcccctgt tgcattcacg cggtccgttc 360 tggctccaga agagctgctt ctgaaagcgg gcggttatgt atcgaccgtc gagcctatcg 420 gaagatgcgg gatgagtgtg gtggtatctt accagcagcc tctgcggaac acccttgcgt 480 tcaccctgat cgcgcctgcc gccgaggaat cgaagcaatc aaatgccgtc ccaggcatct 540 acctacagga cccgacgtgc ggaatgttca attgtgcagg aatggcacgt ggacgactgc 600 gtatgcggtg tcgtggcggg gtccgacgag atgttcccgg tgccgcgcag ctcctgcgag 660 acggacgagc gagggaggcc ctgagcaacg atggacccag gagggcgagg tgggaggctg 720 atgaggtgga tgcgcatggg 740 // ID HO747720; SV 1; linear; mRNA; EST; FUN; 748 BP. XX AC HO747720; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_G10_068 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-748 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 23afb37e15b8c30928d02503c73b50bc. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..748 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 748 BP; 130 A; 254 C; 223 G; 141 T; 0 other; ggtcttcagg tagccgctcg gttcaagcaa atgaacatat ccaccgtcgt catcgagcgg 60 gaagccagaa tcggggacac ctggaggaag cggtaccctt ctcttgctct tcactcgcct 120 cgcaggcacc ataccttgct gtaccagccc ttcccttcaa actggcccga gttcacacct 180 cgcgacaagc tcgccgactg gttcgagcac tacgcctcca tccaggacct cgtcgtctgg 240 acgagcaccg agctcaagcc ccgcccgacg tacgacgccg aacatcggcg ctgggacgtg 300 atcatcattc gcaacggcgt cgaagtcaag ctccgcccgg ttcacctcgt cttcgcgact 360 ggcacgctcg gccgccccgc ggtgccggac gtgccgggca tggaagcctt ccaagggcaa 420 gtcgtgcact cctcacggtt cccaggcggt gttctgttcg cgagcaagca cgccgtcgtc 480 gtcggtgccg ggaacagctc gatcgatgtc tgccaagacc tggctctgtg cggcgcggcc 540 tcggtcacga tggtgcaacg ctcgacgacc tgcgtcatac cgcgtgacta cgcctgcgac 600 gcggtccggg ctgcgttccc tgaagacgtc ccgctcgagg tttcggactt ccgatttctg 660 tcaatgggct tcggccagcg caggaagatg gccatcgaga ggcaggccgc cgcttggggg 720 gcttgcgagg atctgcacga gaagcttc 748 // ID HO747721; SV 1; linear; mRNA; EST; FUN; 823 BP. XX AC HO747721; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_G11_083 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-823 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; ef38f79f2a603f81c0db9451d4c7213b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..823 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 823 BP; 102 A; 321 C; 192 G; 208 T; 0 other; ctctctcaga cccaacgcca acacaccgtt acactctctt taccccttac actctttaat 60 acaaaatgtt cttcagcaag ctctccgtca ttgccgccgt cgccttcggg gctcttgcct 120 ccgccatccc cctcaacacc cccgtcggtg accttcccgt ccccgacctc tccgccgtga 180 ctggccttac gagtggcctt acgggcaacc tccccgtccc ggctctcggc ggtgtcctcc 240 gccgcgatac gcccaagagc attgccgtcg tcctgactgg tgtccaggct cagcttgagc 300 ccgtgactgc ttccctcgct ttcgtcactg ctgcgaacgc gacggttgag gctgtcaccg 360 gtcccgtcaa ccagatcgtc agcatcctcg gcggtgccgt tgtcgacctc aaggcccttg 420 tcggtgttga cgccagcatc attctcgctt ccgtcgatgg cacggccgcg attacagtcg 480 acgtccttgc cggcctcgtt gcgcacatcg tcgtcaccgt gttctacgcg cttggtgctg 540 tcgtcaccgt cgctggctct gcgatcgacg gtgcgctctt cgcgctgctc gctaccgtcg 600 gcgcggctgt cggcacgctc gtcgccctcg tcttccagct cgtcgctggt ctcctctttg 660 gcctcgacgt tgccgtcctc ggcctcatcc gtgccgttgt ccccatcatc atcgacctca 720 acgtccacgt cgtcattgac ctccttcaca tccagtaagc gctctccgcg gccttgggtc 780 ccttcctcca tactccacgt cttctctgca tcttcgtcgc ttt 823 // ID HO747722; SV 1; linear; mRNA; EST; FUN; 631 BP. XX AC HO747722; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H01_001 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-631 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 9737b3adbf9a43093e9dd45820139b6d. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..631 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 631 BP; 148 A; 180 C; 166 G; 137 T; 0 other; ctcctcacga atcatgcagt ttatctccaa catcgaggtc gtctacggcg acgagccctc 60 ccaagctccc tctggccgcg tcctcgagac caaccaccag aacgacgaca tcaactacca 120 gtttggcgga aaatacgtct ggctcgtccc tgtcttcacg accgaccgcg acgccgcggt 180 cactggtttc gacatcgcca tccaagggag cgccgacggc tcgctgagcg acctcgccaa 240 gggtgcgggt ggcgactacc gctacctcct tctcgagaag agcgccacgc agacggagag 300 ggttactgag gttgctctgc tgcgtcttga caatgcgatg accgagcgtc ctgcggggtg 360 ggctggaaac actatcgaca tcaacaaagg ccgcggcaag agctatttgt acctcctgtg 420 gaagaccgct tgaagctctc gttcttgtta accattgaac agctatcaaa cacttcccgt 480 agaactgtgg cttgccagta gccaggatgg cgatgtatct gagtgtcccg aatgtcgtag 540 agttgttgtt gctttgtcgt tgtgaatgtc aagtgcgtta gcgttgtcca ataaacccgc 600 cgtgtttgag ccaaaaaaaa aaaaaaaaaa a 631 // ID HO747723; SV 1; linear; mRNA; EST; FUN; 788 BP. XX AC HO747723; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H02_002 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-788 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; caa35e57a20185a79844ded92c1954ac. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..788 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 788 BP; 157 A; 255 C; 227 G; 149 T; 0 other; gacgaccttc gtgccgtaca actataagcg cgacacggtt aggatcacta caacggacta 60 cttctcggtg ggctcggtca tcgtgttcga cgcgaaccac ctgccgtatg gctgttccgt 120 gtggcccgcg ttttggacga agggcgagaa ctggcccatt ggcggcgaga tcgacatcat 180 tgagggcgtc aacctcatga aggccaacca gatggccctg cacgcgcaga ccgggtgcac 240 gcaggcgtca ggcgtcacac agagcggcac caccggcggc acgaactgca cggacggctc 300 agggtgcacc gtcggggaga accaggcgaa cagctacggg gagaacttcg cgaacgcggg 360 agggggagtc tgggccacgc aatttgacgt gtctggtatt tacatctggt tctggagtag 420 atctaacgtc ccaacctcgg tctctagtgc gaacggcagc gtcgacatct cctcgtgggg 480 cactccctcc gcatcatacc ctgcctcgtc ctgcaacatt accgagttct tcacgcccca 540 acagctcgtc atcgacatca ccctctgtgg cgactgggca ggcgtcgcga gcgtgtacca 600 gtcgacctgt gggggcgacg gctccgcgac ggcgtgctac ctcaacaacg ttatcaacaa 660 cggcacgaac tacacctacg catacttcga catcaactac gtcaaggtct tctccctcac 720 tgacggcgtc cttgtcccgt ccgtctcggg tgggtccacc gtcctcgtca cccaaaccgg 780 gtccgctg 788 // ID HO747724; SV 1; linear; mRNA; EST; FUN; 619 BP. XX AC HO747724; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H03_017 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-619 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 14a89425e9ac734eca62961100766632. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..619 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 619 BP; 151 A; 173 C; 162 G; 133 T; 0 other; agctctcctc acgaatcatg cagtttatct ccaacatcga ggtcgtctac ggcgacgagc 60 cctcccaagc tccctctggc cgcgtcctcg agaccaacca ccagaacgac gacatcaact 120 accagtttgg cggaaaatac gtctggctcg tccctgtctt cacgaccgac cgcgacgccg 180 cggtcactgg tttcgacatc gccatccaag ggagcgccga cggctcgctg agcgacctcg 240 ccaagggtgc gggtggcgac taccgctacc tccttctcga gaagagcgcc acgcagacgg 300 agagggttac tgaggttgct ctgctgcgtc ttgacaatgc gatgaccgag cgtcctgcgg 360 ggtgggctgg aaacactatc gacatcaaca aaggccgcgg caagagctat ttgtacctcc 420 tgtggaagac cgcttgaagc tctcgttctt gttaaccatt gaacagctat caaacacttc 480 ccgtagaact gtggcttgcc agtagccagg atggcgatgt atctgagtgt cccgaatgtc 540 gtagagttgt tgttgctttg tcgttgtgaa tgtcaagtgc gttagcgttg tcaaaaaaaa 600 aaaaaaaaaa aaaaaaaaa 619 // ID HO747725; SV 1; linear; mRNA; EST; FUN; 526 BP. XX AC HO747725; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H04_018 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-526 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 69cf232e47c0b53af3efed8518358af3. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..526 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 526 BP; 122 A; 180 C; 165 G; 59 T; 0 other; acacctacag gaaacgagga cgactccgat gcagacgtac ccgcgcccaa acgcgcacgc 60 accgacgccg cgccggaggc gacctccgcg gaagacacag ccgacacgaa atcagaaccg 120 gcgatgaggt ttatgccgcc ttcaaagcgt ccttgagcgc gtcccgcgcc cagcctccgc 180 cacaaggccc tgtcgtcccg gagacagtca agatcgttaa acgttaccgg tttgcgggag 240 aggagactat agaggtagtc gacgttcccg cggactcgga agatgccaga aagtggccgc 300 gctggacgcc ctcggccagg gcggacagcg gcgacgcgcc tggcccgtcc acaggcacct 360 caaagacgga gcccagtggc ggggccccgt ctgcctcctc tgcgacaccc gtgaacccag 420 cgaagacccc tgggaggcgg ccgggtcccc ggaaggccaa gagcaaactg gcggacgtcc 480 ccacgaagaa agagcccgtg aagaagctaa cgacgctgga caagtc 526 // ID HO747726; SV 1; linear; mRNA; EST; FUN; 769 BP. XX AC HO747726; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H05_033 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-769 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 6d67845a092006f1d19699220c8b3771. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..769 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 769 BP; 197 A; 186 C; 239 G; 147 T; 0 other; aacatggata tcgacaagct tttcaaggtg cctaagctac ccgccggtgg taacaagcgg 60 cgactacctg acaatcctac cccggagatg ctgaagcgtc tgaggatgga tgaggagggt 120 aactctgttt ccacgactcc ttccgaaaac ggcgcaccat cccgcgacaa ggggaaatac 180 cgggccaccg tggaggatga ggatgagggc caggactcca tggacttcgc gccgggcgga 240 gatgcggact acttcgcaga ggaggacgac gaggggaggt tctttggtgg tgggttgacg 300 aaagagcaga aggacatcct gaacatcttc gaccaagcgg gagaggatga cgtgagagag 360 gatctagagg agatgactgc aaccggagta cggaggttat tgacgcgttt cgagcgcgcc 420 gcgaacaaaa accaagacca gcggtcgaag tatccagacg atcctgcaaa attcatcgaa 480 tctgaggccg acctggatct cgctatcaag gctctccttc cgctggctca gtcaccagtg 540 cttgcgtacc ctgaaatcgt tcgttcaggc gcaatcgagc gcctcgttgg tttgctcagc 600 cacgagaata tggacattgt catcgacgtc gtggaggtca tacatgaatt tacggatgag 660 gatgcaggga atgaccaaga agaagaggag gaagacggta gtcgcgaaga cgcgttgaag 720 cagttggtcg agagtctgct ggccaactcc atcctggaac ttcttgtcg 769 // ID HO747727; SV 1; linear; mRNA; EST; FUN; 568 BP. XX AC HO747727; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H06_034 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-568 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; cf212a9122d7f9888b313b577dad8991. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..568 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 568 BP; 183 A; 118 C; 219 G; 48 T; 0 other; ggatgatgag gacgaggacg atgatgaaga ggatgaagag gacgaggaga tcatgacgga 60 ggagcagaag atggaagagg gcaagcggat gttttccatc ttcgcggcgc gcatgttcga 120 gcagcgcgtg ctccaggcgt accgcgagaa agtcgcacaa gagcgtcagc tccaacttct 180 ccgcgagctc gaggacgaag acaagcaaag caaagaacgg gaggccaaga agcagtcgca 240 aaaccagaag aaaaaggata agaagcgaca acagaagctg gcgaaggagg gagaacgggc 300 tgctaaagcc gctgagaagg cggccgaaga ggctgagcaa cgagccaaac aggctgctgt 360 cgaggaggag cagcgcaagc gtcgcgaaga ggagcgcgcg aaacgtgaag cccagcgcaa 420 ggcgcaggag gaggagaagg cgcgaaagga agaagagcgg cggaagcgtc ttgcggaaga 480 gcgggaacgc gaagctgaac gtgaacgcaa gaagcgcgaa cgcgaggaga aggctaaggc 540 tgagcgcaag gagcgggagg agcgagag 568 // ID HO747728; SV 1; linear; mRNA; EST; FUN; 713 BP. XX AC HO747728; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H07_049 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-713 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 533ab6351d721a4aa0658a961ca7b984. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..713 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 713 BP; 148 A; 251 C; 169 G; 145 T; 0 other; gatgggcttg acgacgattc gagatatcat cgccgaactc aaccgccagg ccgctgagca 60 ccaagtccac cgtctgctgc tcatcaagca gatcacctcc gaccccactt atcagcgcga 120 agtcaagcgg ttgcaagaga gctatggctg ggactcattt catggaggat ccgatggctg 180 gcgggtcttc ctccagccgt cgtcggacgg ggatggaccc cacttcagcc ctgccgtgag 240 tgcgctcatg aatcaagtcc acatgaacga ggccacgctg tcttgcatcc gcgcacggat 300 agaggccctg acaacattcc tcctccccgt cttgcgactc ccacaagaag tgcttgagat 360 gatcttcaag gcggggagcc gcataccgca agacgaccct atcctgggat catccattcc 420 ccgtccctta cccctcgtca ttgccgccgt ctcccaccaa tggcgcgata tcgccctcaa 480 cacgcctgat ctctggacga acatcgtaat caaacatccc aaaccgttcg gatgggtccc 540 gttgtgtctc cagcgctcgt gcaacgagcc catagacatc accattgatt cccgctcggc 600 gagtctcctc acaacgcctt tggttgacca gctcctcaca aggctacttc cccacgtcgg 660 ccgatggcgc cgatttgcgc tcacgacctc cgaccctgcc accatctata ccg 713 // ID HO747729; SV 1; linear; mRNA; EST; FUN; 520 BP. XX AC HO747729; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H08_050 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-520 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 0918b3cb50f6ba853dfd1c84465709bf. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..520 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 520 BP; 142 A; 134 C; 154 G; 90 T; 0 other; gttcttcggc aagccgcccg cccccgcccc tggagcgaag gcgccgccga agcagccgga 60 ctttgcgggg gcgcggaaga ggaggctgta caagccgggg tcgagcaagt acgacgacct 120 gctgccgccg aactacatcg acctcgtgcc gaaggaggcg ctggagcagt tcaagagcga 180 gagcgagacg ttcgagtgga cgaagattcc ggagtgggtc ccgccgaagg agctgcgcta 240 gtgagtagtg ggagggtgac tcagcggacg atccgacatg gcacgattcg gaagcgagga 300 agcgagggtc ctgggggcca acgcttgtaa ctgctcgaca ttcttggatg acatcacgct 360 agtatggcta tggtttatta tgaacacact ctgcacgcat gtacatttat ctcacacact 420 tggtacccac aggctcgctc tactggcaca tatagtactc gcaatgtatc cattttgaca 480 acgcaagcca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 520 // ID HO747730; SV 1; linear; mRNA; EST; FUN; 457 BP. XX AC HO747730; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H09_065 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-457 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 056e3ec74aa94a266ce815cbe1610d49. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..457 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 457 BP; 100 A; 105 C; 156 G; 96 T; 0 other; tctctcgggt cgtctatcga aatccgctct gtggagggcg ggtttaagga ctccttcgac 60 gaggacggca gtacgagtct ggacctcacg atccaagtcg gggacctgtc gaccaaaggc 120 gggaagtctg ggggtctgga ttctggggtc gactctgcga tggagatgac gccgctgtcg 180 gcctctgaga cggagtcgcc gcggggggtg tcaggtccgg agcggttgtc gctggtctga 240 gcgtcctgac agtggcaagc gtgccagcac gctcagtctg ttcctgggcc agcttcgatc 300 tcagatcggt ctaagtctga aaggtctgcg tgctgtgtcg aagttgtata catggaagaa 360 ggccgaagtt ccgtgagatg cggagaacgc tgcgcttggg gaagcccggg aatatcgact 420 acgttggcgt gtgtaaaaaa aaaaaaaaaa aaaaaaa 457 // ID HO747731; SV 1; linear; mRNA; EST; FUN; 606 BP. XX AC HO747731; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H10_066 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-606 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; bd66487fc6f0dc991e718e77fbac1a8b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..606 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 606 BP; 143 A; 170 C; 161 G; 132 T; 0 other; tcacgaatca tgcagtttat ctccaacatc gaggtcgtct acggcgacga gccctcccaa 60 gctccctctg gccgcgtcct cgagaccaac caccagaacg acgacatcaa ctaccagttt 120 ggcggaaaat acgtctggct cgtccctgtc ttcacgaccg accgcgacgc cgcggtcact 180 ggtttcgaca tcgccatcca agggagcgcc gacggctcgc tgagcgacct cgccaagggt 240 gcgggtggcg actaccgcta cctccttctc gagaagagcg ccacgcagac ggagagggtt 300 actgaggttg ctctgctgcg tcttgacaat gcgatgaccg agcgtcctgc ggggtgggct 360 ggaaacacta tcgacatcaa caaaggccgc ggcaagagct atttgtacct cctgtggaag 420 accgcttgaa gctctcgttc ttgttaacca ttgaacagct atcaaacact tcccgtagaa 480 ctgtggcttg ccagtagcca ggatggcgat gtatctgagt gtcccgaatg tcgtagagtt 540 gttgttgctt tgtcgttgtg aatgtcaagt gcgttagcgt tgtccaataa aaaaaaaaaa 600 aaaaaa 606 // ID HO747732; SV 1; linear; mRNA; EST; FUN; 701 BP. XX AC HO747732; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H11_081 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-701 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 79472325495e508ae6c7e7102c4578c7. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..701 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 701 BP; 163 A; 224 C; 163 G; 151 T; 0 other; tcaagcgtac cctccccgtc ttttgctatg gcggtatcaa gacatacctc ttccttgtca 60 ttctcctcgg catccccatc acagcaggct actggactct catgagcacc tacggtcccc 120 gcaagaacca aaaggttcaa ctccccggtc gtaacatcga agagtacatc actatcaagg 180 accccgagct caaggccaag tactttggca aggagaagat acccatgcag gtcttccacg 240 acgctttctt tgacgacaag gtcgacttca acggcgacgt gcttgacatc ctcgagtctc 300 gccacgactg ggccaagatg gtcttcactc ccgaactctt caaatacgtc cttacccaga 360 tgcttcccga ggtcatcatg cactccaagt cccaggatga ggagcaggtc cgcgaccact 420 acgaccgcgg agacgatttc tacgaatggt ttctcggtcc ccgcatgatc tacacctcgg 480 gtattgtccg tgaccttact gtcgaagagt ctcttgagca gctccaggac aacaagctca 540 ccgcagtctg tgagaagctt gacctcaagc ctaccgaccg cctcctcgat gtcgggtgtg 600 gttggggcac tctcgccgca tttgcgcaca agaactacgg gtgcgaggtg accggtatca 660 ccctcggcaa gaaccagacc aagttcggta acaaccgcat c 701 // ID HO747733; SV 1; linear; mRNA; EST; FUN; 612 BP. XX AC HO747733; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050051.T3_H12_082 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-612 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 01ab3181a9912458372015dc8eb5da50. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..612 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 612 BP; 113 A; 152 C; 246 G; 101 T; 0 other; ggaaagacgt cgacggtctg tcggaggagc aggaggagct aggggatgtg ccctgggatg 60 agatgtggga gaagagcgag tacccgttcg tccgcctcga gggcaaccgt gccgtgtgcg 120 ccatctgtct catggacttc gaagaaccta agcgcgtccg cggggcaaag ggctcgggag 180 cgaagtcgag tgcgggagta ggcgccaacg cggagcccgg gccatcctcg ccgcccgtgg 240 gaggagggag cgagagtcag agtggcactg ggagcgtggg cggggaggca acgcaggaga 300 tccaggtgga ggcggtgacg gaggaggagc gggccgcgct gggggaaggc gaagggccgg 360 agccgctgcg gctgctgacg tgcggacatg tctttcacaa aacgtgcttg gacccgtggc 420 tgacggacgt gtccgggcga tgcccgatct gccagcggcc ggtggaggtg cccgagccga 480 cgaagaagtc gaaggggagg cgtcggcgaa ggacatagtg cggcgtttgt gatgtgcgac 540 gacggctgac tctcgttttt tacacgctcg atatttcttt gtgttctctc gttctcgcac 600 gtcggactac ag 612 // ID HO747734; SV 1; linear; mRNA; EST; FUN; 815 BP. XX AC HO747734; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A01_015 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-815 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 9d61de16f2f0746ef972355fd34a3ce3. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..815 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 815 BP; 160 A; 278 C; 171 G; 206 T; 0 other; cagcagcctt tacgacgtcc tcgacgactt cagtggccca gtccgtcctt actgcattcc 60 gagcaaccac attcgttact tcgcttttca aagtcatgtt ctcccgctct gtcgtcgctg 120 cccttgttgc tgctcccttc gtggccactg cctttgccgc gtcgtgcact cgcacgtaca 180 ccgtcaagga aggtgactgg tgcgatacca tctccgctgc caacaatgtc tcaacctacc 240 agctcgctgc tgtgaacacc gccatcgata acctctgcga caatcttgag gtcggccaga 300 acctctgcct tggcactcag ggtgaggact gctcgaacac tcatgttgtc gaggccaacg 360 acacgtgcga ccaactctac aacacctatg gtatcaactc caccattctc taccacaaca 420 acccccagct caccgcggct tgcgacaata tgtacattgg cgaggtcatg tgcgtcgcga 480 acgttgctct tgccccggca cccccgtctg gcctctcagt ccctacgacg agcagccagg 540 cccctgccac gacggagacg gagcttccct ggtgcgacga gctctaaatg actttcgtta 600 gtcatttgcc accacttccc atacagtctg gcctatggtt cgtttaccct acccgctgat 660 acctgcattc ccactctcgc gaggtaacat gcagccatac taccttacac attgttctcg 720 ttttgtcatt aaccctgcat acccgcacga ccctgtgcat ttatctgttg ccgcgcacca 780 ttgtctttta cttcgtagat agttcagttg gacta 815 // ID HO747735; SV 1; linear; mRNA; EST; FUN; 539 BP. XX AC HO747735; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A02_016 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-539 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 78524257dd52e5227fb8ca5bee22d64a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..539 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 539 BP; 74 A; 211 C; 161 G; 93 T; 0 other; acgaccacgt tcgtcaaccc cttccgtatc aagccccgga agcccaaacc ttccctcgcc 60 ccgaccaacc ctcctccctc ctcagtccct gtccctgctt cgcgccgcgc atctggccct 120 cgccgaatgc tcaactccct ccagctgacc ctcgccggca acgcctccca cgcgtctatt 180 ggtcctgcac ccgactcacc ccgctcaagc atctcgtctg cctccacgtc gacgttttcc 240 gcgtcatcct cggtcaggtc tgcgttctcc ggctcgtccg gctcgccgcg gggcgtgcga 300 cgcacgcaac catatgccgc gccgtactac gctgccatgc ccgggtctgc aggcacgact 360 ccgggttcct cggcgcggag gcgggccgcc tcgtgctcgc gcgagtggcg gccggagcca 420 gagccggtcg aggaggaggg cgaggactct gatgcgaacg cgctcgggct cgagttcgcg 480 tcgtcagagt ctgagttcgg ccagatgcgg agggggaggg ggagcacgcc gctgcggga 539 // ID HO747736; SV 1; linear; mRNA; EST; FUN; 755 BP. XX AC HO747736; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A03_031 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-755 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; c68efe2c84368896ca756825988acef9. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..755 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 755 BP; 163 A; 204 C; 204 G; 184 T; 0 other; gagactatca agtttgcatc cgggctcgct cgttcggcta tattccgaag tgagtctcta 60 cttttggccc cccccgacat cgtgacacat ggaaacatgt ccttcatgcg accaagcgga 120 agatacgtgg cacgagcttg caacgcacta cgacgactct tctttaacac ctagctgaag 180 atgcagatac ctctttccag gaagaacagt gcatgtcagc gccgatttca aggtacggaa 240 gatcccgatt ggacccgcgt ctggtccatg gcgagccatg ccagtccgga gcaaatgaag 300 aaagtccctt gaagctttga tttgcgtagg gatcgtcacg gggagcctct tgttgtatcg 360 attccctgtt ggggctaacg gtgtttgagc cactttcacg gttggaatta tccgcacggt 420 ggctgccccg agcttgactc gacggacttg taggatttgg gcctgctcgc agctgtcctc 480 gcgggagatc ggggtgggtg ttgcggcttg catcgtttct acccgtgggt gccatccaca 540 cgaggagacc aagagtctta taaacgtttc tggccgcttt gacacaagcg agggaggcgc 600 ttcaaccatc tttggaatgt atcatctcag agtctagatt gacctattta cctccattcc 660 atcaacgcac aaaagcgggc tatggacctt tgtcccacgc ttgaagaact gtcccgtagt 720 ggccacggtg cgcaacggcc aaagcgtcag cgctt 755 // ID HO747737; SV 1; linear; mRNA; EST; FUN; 693 BP. XX AC HO747737; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A04_032 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-693 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 3d85a5b2bb50b5938e152da1d6113a89. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..693 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 693 BP; 124 A; 228 C; 207 G; 134 T; 0 other; ggccccatcc aagagcgtct ccccttcgtc cacttcaccc tccactagta ctactcgcga 60 ctgcaaaaat ggcctcagtc aaggtcggcg acgagatccc cgcgggcgtg ttcgtccacg 120 tcccgttcac gcctgagctc gaggagcaca gcgcatgcgg catccccacg aagctctcca 180 ctgacgcgtg gaagggcaag aaggttgtcc tcttcgccgt ccccggcgcc ttcaccccct 240 cgtgccacgc gaaccacgcg ccgccgtacc tcgcgcaggt gcccgcgctg aaggcgaagg 300 gcgtggacgt cgtcgcggtc gtgtccgcga acgacccgtt cgtgctctcg ggctggagcc 360 gcatcctcgg cttcaaggag tccatcctcg cgctcgcgga ccccgatgcc gcgtggtccg 420 ccaagctcgg cctcaccgtc gacctctcag ggctcggtat cggcttgggg aagcgcacga 480 cgcgctacgc gcttgttatc gacgacttga aagtcacgta cgttggggtc gagcctgacc 540 cgactaaggt cactgtttcg ggtgtcgacg cggtgctcgc tgcgctctga aggggtggca 600 gtggttgacg cagaagaaga tagacaaggc atagcaaggt gtcgtgttgt acgttgcaat 660 gactttccat gtaatacaga attgtgaacc taa 693 // ID HO747738; SV 1; linear; mRNA; EST; FUN; 596 BP. XX AC HO747738; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A05_047 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-596 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 8b852df53c6a8f55afd9870659a97dbe. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..596 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 596 BP; 132 A; 161 C; 153 G; 150 T; 0 other; tctttcttcc ctccgccttt tcacttgcac actggctgtg caaaatttta gtctttccta 60 actgttcaag ttcgttaacg acatccacct ttacatgtaa acgaacccca aactctctgc 120 aaacatgcat ttctccactg tagtcgctct agctctcgtc cacttcgtcg ctgcggcgta 180 cgttccccgc acggaagccg attcgggcct cgttgccgac gacgtcgaga tcaacgccgg 240 gtctttggtt gcccgccacg gcactgtgcc catcggccaa cgctgcagca gcgattccca 300 gtgcgcatcg acctgttacg ggttcagcac gggaaagtgt gcgagtgttg tcgttgccca 360 gccgctcgac ggtggttgcg gccacggcgg tgctcacccg aagcacaagc tccgtaacct 420 ggactaagaa tcgaaggcgg cggcgtggtt gttatggttg gtaacgtttg gggatggtga 480 cattattagg aaggctgagg gtttggagta acttattgtt agtattcaag gcgggcgctg 540 ttagagacgg aatgcatcct acgcttcatt taacatcaaa aaaaaaaaaa aaaaaa 596 // ID HO747739; SV 1; linear; mRNA; EST; FUN; 812 BP. XX AC HO747739; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A06_048 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-812 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 196dfab8429539545da58f95d7607cf1. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..812 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 812 BP; 236 A; 160 C; 169 G; 247 T; 0 other; tgcagaacat tgctttttaa acaactggca gggccttcgg aataaggaag ttataattga 60 ggacacttaa atataagtga ggcagtgatc caggaagcct gtattttaaa agacaaaggg 120 acattcatga attggagagc attgcttttc agggctccag gactcagaaa cttgaaagca 180 atctgaaatt ccagtagagg tgagttccaa tttgattttt ctgggattaa ttcattaaaa 240 gctaaaaact gagccctcac tcataacctg atatgtaatt tcgaggagta tagatgaccg 300 accctggagg ctctgttctg ttgttttacg caaatcttca ttttctagac caagccccac 360 tctggtgttc gggatctgct tgcagacact cgtgatttgt agggtcatcc aaagcagttt 420 tctcacctgc agtcagtttt catcctcagg ggacatttgg taatgcatag aaacattttt 480 ggttgtcaca atgcgggggt gctattggta tctagtgttt agatatgttt ccaaacgtct 540 cataaagcac aggacagctc cacacaatga agaataatct ggcttcgaat gataatactg 600 tgatcaaaat caatgatttc caaagagtag tccccaaact agccacttca gtactggaaa 660 catgttagaa atgcaaattc tttgcctcca tgtccatcca acagcatcag aaacttgagg 720 tggagcccct gcaatctggg tataacaagt tctccaggta actttgatgt gccttagttt 780 aagattcttt ccttattaat gttttctgtt tt 812 // ID HO747740; SV 1; linear; mRNA; EST; FUN; 466 BP. XX AC HO747740; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A07_063 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-466 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; ddf170e8e9f0d7177d0d7f33ed635e27. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..466 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 466 BP; 132 A; 101 C; 125 G; 108 T; 0 other; ccaaagtttc gatcgcgccc tcggcgcaaa cctactctac aggtttgagc gaccgcagta 60 cgcagagatc cggaaaaagt acgtcacagg cccgacggtg caggtcggcc aggagaaaga 120 gatgagtgca atatacggcg ccgaacactt ccttcggatg ctcgtgagct tgccgcagat 180 ggtcgcgagt tcgtcgatgg acggggaatc agtagggctc ttgagggatt acgtgaacga 240 gttgctacaa tggatggtga aggaacgaaa ccggatattt gtgccggaat atgaaagcgc 300 aagcatcgca taccaaaaca tttcgcggtc atgatgtcga cccggctttg gctgtatgta 360 tgtatgtact acctatgttt gccttgtgct tgccgtgtat tgtattttcg gagtcaattc 420 tgagtaattc ctttcacgat caaaaaaaaa aaaaaaaaaa aaaaaa 466 // ID HO747741; SV 1; linear; mRNA; EST; FUN; 777 BP. XX AC HO747741; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A08_064 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-777 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; a87e6951b6c08f38bec3a0046c63ae4d. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..777 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 777 BP; 181 A; 272 C; 183 G; 141 T; 0 other; ctctttgcct cctccacaca ctctccacac actaccacca ccatgccaga ccaggccccc 60 gcagcccagc ccgcagcggc cgccaaatcc aagcacggac atgccgcgtc ctctgcacaa 120 cccgcaccac gcaaagtgcg atttaatgtc ggcacccagt accaggtcct cgacgtcgta 180 ggagagggcg cgtacgggat cgtatgttcc gcaatccaca gaccaagcgg ccgaaaagta 240 gccatcaaga agatcgcgcc tttcgaccac tcaatgtttt gtcttcgaac cctccgcgag 300 ctcaagctcc tcaagttcct tagcgaagcg ggcgtaagcg aaaacatcat ctcaatactg 360 gacataatca agccaccatc tatagagcag ttcaaagaag tctacctgat ccaggagctg 420 atggagaccg acatgcaccg cgtcatccgc acgcaggatc tctccgacga ccacgcccag 480 tacttcatct atcagaccct gcgcgccctc aaggccctcc actccgccga cgtcatccac 540 cgcgacctca agccctcgaa cctcctcctc aatgccaact gcgatctcaa ggtctgcgac 600 tttgggctcg cgcgctcggt gcgcaccgcg gagccatcgg gaacagagac ggggttcatg 660 acggaatacg tcgcgacgcg atggtatcgt gcgccggaga tcatgctcac gtttaagcag 720 tacaccaagg cgattgatat ttggtccgtc ggctgcatcc tcgcggagat gctctcc 777 // ID HO747742; SV 1; linear; mRNA; EST; FUN; 750 BP. XX AC HO747742; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A09_079 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-750 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; dea0e836ddcccec1bee145d1cc4cb224. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..750 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 750 BP; 136 A; 277 C; 204 G; 133 T; 0 other; ggccattctg cccatcaacc ccgatcgcgt cgtgcgtagg gcaatcgccc gcttcgggtt 60 gtctatcgag caggagatcg agatcaactc ctctgttcct acgtctctcc cggtcgggaa 120 ggccatcacc atccacaacc tcctcaaggg ctacgtagag ctccagcagc ccgccaccca 180 gcgcgacctc gatatcctcc tcaaggccaa gaactcggac gcatccacgc agatcttgaa 240 ggacctctcg gccaactacg ccgagaaagt cttcaagaag cgcctcagcg tgctcgacat 300 cctcgaggag aacaaggaca tcgacctccc gctctcgacc ttcctccaga tgctcccgtc 360 catgcgcatc cgccagtact cgatctcgtc ctcccctctc tggaacgcac agcgcgtctc 420 gctcactgtc agcgtcgtcg acgccccagc gctctccggc cgcggcgagc ccttcctcgg 480 cgtcgcgtcg acgttcctcg cgggcctcca gaaaggcgac aaggtgcagc tatcggtgcg 540 cgcgtcgaac gtgcacttcc acccgcccac cgaccccacg atcccgctcg tcatggtcgc 600 ggccgggtcc gggctcgcgc cgatgcgcgg gttcctgcag gagcgcgcga tgcagaagct 660 cgcggggcgc gaggtcgcga agaacctcct cttcttcggg tgtcgcaacc cgctggagga 720 ctacatatat ggggactcgg acttgaagga 750 // ID HO747743; SV 1; linear; mRNA; EST; FUN; 698 BP. XX AC HO747743; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A10_080 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-698 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; ab9da6829a08366aaabc79fd4d40923f. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..698 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 698 BP; 160 A; 215 C; 146 G; 177 T; 0 other; ttcgttcctc tgctttcaaa ctcttgttct ttttcgctag tcgaatttga caccttttaa 60 cgctttcttc ttcaaccctt ttaaactttc ttttaccctt ttatttttat agtcatgtac 120 aagctccttg tttccgctct cgctcttatt gccttcgctg gttctgctgt ggctgagaag 180 cacactgtca ccttcaccaa ccgctgcggt cgtggcactc ccatgctcat ccagaacggg 240 aaggtcctct cgaagggcaa gccctacacc tccaaaggac ccctcatcgc tgccattgct 300 tacctccaga ctggcaagtg tggcttcaac ggtgacggct gcttgaccct cgagacgacc 360 ctcaagaacc ccaactgccc cggctgtggg tcgtcaacgg acctgacttt gatcccgccc 420 cacaagttcc acatcaagac cggcttccgg tacaagaacg gctgcaacgg tcacggcacg 480 acctgcacgg gaccgcgctg cggcgtgaag aatgcgttct acaagacaga cgactacacc 540 gcccagcgcc agtgcgagac caaggacgtt ggtctcgagg tcatcttctg ctgagcttgt 600 cgctgcgcgt caagatgctt gtatgttact atacccccaa ccttattacg tctaatgcag 660 aggaacacaa aacacttgtc aaaaaaaaaa aaaaaaaa 698 // ID HO747744; SV 1; linear; mRNA; EST; FUN; 778 BP. XX AC HO747744; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A11_095 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-778 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; e6d8309972045f4a2d3768c8b570b63b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..778 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 778 BP; 136 A; 294 C; 164 G; 184 T; 0 other; gggtcgacgg tcccatctcc gtcccgccgt cccgctcttc agcacgtagc cctcttcccg 60 tccgaccctc acccgtctcc ttgcctacac catgtatctc tctaacgtgc tcttcgcctc 120 tctcctcgct tccgctctcc agctctctca agtctccgcc tctcgctcct ccctccactc 180 ctctcacgcc cgtcgctccc tcgccttcaa gagggaccac atctcagagc tcctccagca 240 ccgcgacctt cttagtggtg tcggaaagac tctgggcgat cttggagatg gtctaggagg 300 cgtgatagga actacaggca acgaccttgg aaatattgtg gatgggctca cgtccctggt 360 tcccgcaagt tcaacgaccg tttcgtcgtc atcaacatct gcgtcagtga catcgaccga 420 ctcctcgtcc ctgtcgtcaa ccagctccgg gtccgcgtct gcaacatcat caaattcaac 480 taccgcaatc tccacgacat ctggatccac atctggatcg tctacaggct ccgcatccat 540 aaccgatacc caaacgtcca tttcctccac gacgacggct tcggcgactt ccacctcgac 600 ccctggtgac ccattgtcgt cctggctatc gtccattttg agcccatcga ccacggagtc 660 ttcaagcaca ccatctgtca cttcatcgcc cactgaatct gggaccggtt cggggacggc 720 gaccagtcct gcgactggct cggctacctc caccgactct ggttcggcca gctctgct 778 // ID HO747745; SV 1; linear; mRNA; EST; FUN; 717 BP. XX AC HO747745; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_A12_096 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-717 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 5c8a7c805405225f4dfc096eeaf07a5d. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..717 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 717 BP; 160 A; 235 C; 219 G; 103 T; 0 other; ggacctctcc aacttgcaca tcttcctaca gctttcgtat ctatagtcat gactcaaggc 60 tgggagcccg gaacctggta cgaccacggc gctgtcgtcg agtacgaagg ccacaagtac 120 aagatcatcc agccccacaa ctcgcagtcc gattggacac ctcccgtcac ccccgcgctc 180 tggggccgca tccccgacga cgaatgtggg gagcaccagc accacgagca acagggccag 240 cagtaccagc cccagtacca gcagcccatt ccgccgcccg gctacaaccc cggccagagc 300 agcggtggcg agaagcacga gcagaccgtc accgttcacg aggaggagcg caagcacggc 360 tgggacgctc ttagcgatga gcgcaagaag cagatcgaga ttggcggcgg tctgctcgcg 420 ggtgctctcg cgctcggcgc cggagtcttc gcctggaacg agcacaagaa gagcgaggag 480 gataaaaagc ggcatgcttg ggatgtcacg aactggctgc acgagagcga aaagcgcacc 540 gccgacttct gggccgggcg cgccgctggc ccggtaactt gggtcctggt cgacggcaag 600 aacatccccg ccgaacacgc catcgtcggc ggcgaggagc acggccagcc gcactacatc 660 tgccgtggtt ggcacgaggg cagtctccag attgggaaag catgctcgat cttccaa 717 // ID HO747746; SV 1; linear; mRNA; EST; FUN; 684 BP. XX AC HO747746; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B01_013 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-684 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 73bbfa90c3244bd4f94ead5190337a20. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 684 BP; 198 A; 164 C; 180 G; 142 T; 0 other; ccctcttcct acctcttgag tacattgcac accacctacc gccagggaat cgaggaagct 60 tagaacaaga acccgcccag tatgccgaag aataaaggaa agggtggtaa aaaccggaga 120 cgtggaaaga acgagaacga cgacgacaag cgcgagttgg ttttccgtga ggacggacag 180 gagtacgctc aggtgaccaa aatgcttgga aatggccgac ttgaagcgat gtgcttcgac 240 ggcgagaagc ggctggcgca tattcgagga aagatgcgga agaaggtctg gatcaaccaa 300 ggagacatca tccttctgtc cctccgcgat ttccaggacg acaaggccga tgtcattgta 360 aaatacactg cagatgaggc tcgtagtctg aaagcgtatg gagagctccc cgaaaatgcc 420 aagatcaacg agacggagac cttcggcgag gaggagggcg agtgcacatt cgagtttggg 480 gaagacgagg aggtcgatat cgacgatatc tgaaggcgtg tatttctatc cctgtatttt 540 tcgcgactgc catctcgatg tacaccccac gacttcaact gtctccaccg ctatttcgac 600 ttgctgtcat tcagtttgtt tatgcccatc gatgtaccac acgcaaatgc gattcgttct 660 tccttcaaaa aaaaaaaaaa aaaa 684 // ID HO747747; SV 1; linear; mRNA; EST; FUN; 665 BP. XX AC HO747747; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B02_014 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-665 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 77a5d38e6b3d596001517a4624b6b48a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..665 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 665 BP; 153 A; 212 C; 173 G; 127 T; 0 other; tctcatcccc ttgcggatgt gcatcccgcc aacccattcg cgaccctctt cccgggccag 60 gactacaaca acgctcgcat catggacttc caggacgtcc ccaactacgt cagcaacggt 120 ctgagctcca tcgaatccgg acgcatgccg tggcacgatg tgcacatgac tctcactggc 180 gaggtcgtgt tggatatcgt tcagcattac gtcgagcgct ggaacgaggt caagaagcgc 240 aagtaccgta ccgacgcgcg gtacgactgg ctggcacttc cacataacat cgaggcgtca 300 cccaatgagg ccgttgtccg ccaccctcac cgcaaccact ggcatgagat aggcagacat 360 ttcaaggacc gcttccactc ctacttcggt gatgaggata gcggacgcac cgcggactac 420 gaccccgacc atccaatcac ccgtggcacc tgccgcgtgc aggttgtccg cagtgtctcc 480 gactggagcc atggtgtcct gacggagtgc agcatccaaa acgcctacat cgagttgatc 540 cggcagtctc aacacttcat ctacatcgag aaccagttct tcatctccaa cacgcaggag 600 gatggtgtgg tgaagaataa gatcgcgaaa gcgctcgtgg aacgtatcgt ccgtgcagga 660 cgaga 665 // ID HO747748; SV 1; linear; mRNA; EST; FUN; 584 BP. XX AC HO747748; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B03_029 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-584 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 38c70ec45eb7f9b40528fd88e7f99252. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..584 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 584 BP; 109 A; 201 C; 157 G; 117 T; 0 other; acagacattg tttgaatcgc tcatcttcat cttttctacg cacgtctttg atgtcggaga 60 ccttgtgatg attgacgaac agcccctgtt cgtccgcgag tttggcctgt tctcgaccac 120 cttccgacgt gtcgatggta tggagatcat cgcacccaac tcgctgctcg cctcctcgaa 180 gctcgtccac aacctccgcc gcagcaactc gatgtgggag tccacgacgg tcaccgtggc 240 gtacgacacc ccccttgagg tgatcgagca actccgcacg cgcctgcagg catacgtgaa 300 cgcgaacaac cgcgagtggt ccggcgtcgg cgtgaacatc gacaagatgg agcaccagaa 360 cgcgatctcg ctcatcgtgg ccatggagca ccgccctaac tggcaggact ggggcggccg 420 ctgggtccgc cgtactgcgt ttatgcgcaa cctcaagaac atcctcgagg acctcgatgt 480 ccgctacaca aagcccatcc agcccatcat cgtgccccgc ggctctgggg gcctcacgtc 540 accgttcctt gacctcccgc cgtcgccgcg ctcgggtggc gcga 584 // ID HO747749; SV 1; linear; mRNA; EST; FUN; 691 BP. XX AC HO747749; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B04_030 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-691 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 16a5f717fbce50cdd844db07a299622d. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..691 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 691 BP; 131 A; 240 C; 173 G; 147 T; 0 other; tttgacgtgg tgactcttgc cgacgcccag cacctctcct tacggcctct ccctaccgcc 60 cccgatggcc gctatgattc tgaggcgcag cctattacga gtgcaacccg gagcgacccc 120 cactccgact tgtacaaggt cgccaagggc ttgaaggata gcctgaaggc tgctactcct 180 cttctcaagg aggcactgga cccaaccaca tatcaggcgt ttgccgacca gctcctccac 240 tcggactctc ttgatgatcg caagaaactg gtgatctcgt tcatctcatc ccgaacgcat 300 cttactcact ggatgtggct gatagttcac gaaaacgctt tcgatcttgt gctggatccc 360 ggagggtacc ccggagtcca ttgcgctgaa caaccaagtc atcaccctgc tctacaacac 420 tctgccgcac ccgccggtga cgtacattgg gaccgactac cctcccggca gccagggtcc 480 cgtgccgtct gtcccctccc ctggactgca gagcgcacct gcctctgcgc gccttccacg 540 caccttccgt agcgcggatg gtagtgggaa caacgtcaac atgccgaacc ttggcaaggc 600 ggggacggcc tatgcccgca gcgtccagag caagtatccg ctttcgtcga gcacccttcc 660 ggaccctggc gatgtcttcg attcgctgtt g 691 // ID HO747750; SV 1; linear; mRNA; EST; FUN; 761 BP. XX AC HO747750; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B05_045 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-761 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; d1720fd3c175bd6f0a9b0051e6284897. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..761 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 761 BP; 181 A; 212 C; 224 G; 144 T; 0 other; tctctcccaa gtcctgtgtt ttccacacac gttctttacc tcccactatc ggggtgactc 60 ttgatctgca cttgttattc cccgattgga ttatggcgac tccgagacac gagttctacg 120 agaccgacga gaacctcacc ctctccatct tcgacaaggg agccgatcca gaacaggtca 180 aggtctcctt tgagcctagg aagttcacct actcccacgg cgataagtct ctcgtgctag 240 agcccctcaa agggcagatc gatacagaaa agagcagctt caccgtcgga aaggtcaagg 300 tcgagatccg cttcgccaaa atggctcagg gacggtgggg tgccctcacc ggtgatgctc 360 cagaccccct tgctgcgttc cccgcgagct cggcgcccac gagtacgaca gtccgcaaac 420 aacggaagaa ctgggacggt attaccactg acatccttgg caaggacaag ccgctcactg 480 gcgaccaaga cccgaatgca gggggagatg ccgccgtgaa cgaattcttc cagaagctct 540 acgcggacgc ggacgaggat acgcgtcgcg ccatgatgaa gagttactcg gagagtggcg 600 gaaccaccct cagcaccaac tgggatgagg tggggaaggg gcgggtcgag gtgaagcctc 660 cagagggaag cgagtggaag aagtgggctg catgagaatc cgggcagagt gattattggt 720 ggagatgctc ggggagttca gagcatgcca cggtttacga t 761 // ID HO747751; SV 1; linear; mRNA; EST; FUN; 482 BP. XX AC HO747751; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B06_046 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-482 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; f7d3f8095ae0919489404a4116c281e8. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..482 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 482 BP; 108 A; 144 C; 168 G; 62 T; 0 other; cgtcctcagg ctatcagcat cctcccaccc accgtcacca cggacagaaa cgcaccttcg 60 catacgtcgt caaatgactg cgcatccaca cccgctcacg cccgtcgacc gcatacgggc 120 tccgctcgac gctaggcggg ggcaccgcct cggggtgggc gtcctggcgg agggtgatgg 180 acgagaagcg ctggaggagg cggacgagca tgaacgagat ctcgttgtat gcgaactgcg 240 agtagagaga aacgcgtcag cggcgagcgg caaggtagga aaccgaagac tcgccgcgac 300 tccgcagggc gagagatgcg gcgacgaaag gtgcgaagcg gcaacggaac ggagcgacgc 360 acctgctggc cgaggcagat acgcggcccg gcgttgaagg gaaggaagat gaacgggttc 420 gggacgaggt acttctgcac gcgctcgtcg atgaaccgtt ccgggtcgaa cgacagcgct 480 gc 482 // ID HO747752; SV 1; linear; mRNA; EST; FUN; 607 BP. XX AC HO747752; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B07_061 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-607 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 532d7e246561499227a33d7f321f0ba0. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..607 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 607 BP; 144 A; 143 C; 190 G; 130 T; 0 other; tttttttttt ttttttttgg tgaccaccac gagaattaac attataagga ccaatacttc 60 gtcgtgaaac catctctggc atgaacatgc ttcgtaatat catatccaca ttagatcttg 120 aacgcgccgg cggggccctg ctggccgatc ttcttctgcg cacttgagaa gcagcggtag 180 gtcgcgagac cgccgttacc gaaggtgaca tcgcagtagg ggaacttctt ctggtcagac 240 ttgctgacgg ccttcttgag agccttggtg gcctccttct tgcgaagagc cgagatggag 300 cccgaggggt catggaagct gatgtggagg ctgctcttgc caccaccaaa gaggccacga 360 gcctcaagcg cagattcgtc gaagagacca cggcggacga ggtactcgat ggcggcggtg 420 tcgatgtcgt tgagctcgac agcggacgag tcttcacgag cctcgaaggc gacggggcca 480 gcgacagcaa cggtggcggc ggtgagggca atgacgacag cggcgaactt catgatgaag 540 attatgtgga cgtgaaaaaa gagttggtga agtaaaggcg aatagacaag gaaggaacgg 600 gctcggg 607 // ID HO747753; SV 1; linear; mRNA; EST; FUN; 613 BP. XX AC HO747753; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B08_062 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-613 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; a2a947ec8f9eabdb17786f5c3be3d326. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..613 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 613 BP; 139 A; 188 C; 178 G; 108 T; 0 other; gtggagtcga cggacaccgc tgttgcgacg ggtacggcta caggcaccgc tgccgagtcc 60 accgacacct ccgccgcgac taccacgggc tcgtccgttg gctctggcgc gaccgcgacc 120 ggtacggata ccgcggtcga gtccaccgga accgctgctg agtcgactgg ggccgcgtcc 180 gctacgggta ccgcggccgc cactgctact gctacttcca cgtcgtccag cagcgatgac 240 ggggacgatg ggagcaacgc gagcactgac ggctccgacg actcgtctgc gcgccgtcgt 300 cgccgctcca aattccccgt caaccgcttg tgagcgcatg ctcggcgccg gtagacctca 360 tatggacctt cccgttgaac gctagtcgta cggaagacgt acacatacgc agtagcacac 420 gcctcagtgt agacgtacat acacgtatgt atgcatagac gaacgcgtac aggactgtaa 480 cccgcagtcg tcgcgccgcc gaagtgcttg gggcgcacgg tgtgggtttc aacttggtct 540 tgtagcataa gttattcttt gaatgacatg gcggacgcct ggcaaaaaaa aaaaaaaaaa 600 aaaaaaaaaa aaa 613 // ID HO747754; SV 1; linear; mRNA; EST; FUN; 816 BP. XX AC HO747754; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B09_077 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-816 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; f23df2647fbade621361e776ba2169c7. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..816 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 816 BP; 158 A; 241 C; 204 G; 213 T; 0 other; ggtctttcct gtcccttctt cctcgtatca taaccatggg tctctccgtc tcccggctcc 60 tttccggtct cttcggcaag aaggagatgc gtatactgat ggtcggtctg gacgcagctg 120 gtaaaaccac catcctgtac aaattgaagt tgggcgaaat tgttaccacc atccccacca 180 ttggattcaa cgtcgagact gtcgagtaca agaacatttc gttcactgtg tgggatgtcg 240 gaggacagga caagattcgt cccctctgga ggcattactt ccagaacacc cagggtatca 300 tcttcgtcgt cgactccaac gatcgggaac gtatctcgga ggctcgtgag gagcttcaac 360 ggatgctcaa cgaggatgag ctccgtgatg ccctcctcct tgtcttcgcc aacaagcagg 420 atctccccaa cgcgatgaac gcggccgaga tcactgacaa gcttggcctc cacggtctcc 480 ggcagcggac gtggttcatc caggcggcct gtgcgacgag tggtgatggt ctgtacgagg 540 gtcttgagtg gctgagcgcc aacatcaagc gacgggtgtg agcggtgtcc tagccgtctg 600 ggttcctctt caacattgcc gaacgttcat gtcttatgat ccttacgata tcgaagaggg 660 tttcactggt tacctccgcc tcctcttgct cacctgtccg ccgcagcctt ttccccgtcc 720 gctaccttga cttttcttct gtcttctgtg ccatataacg taactcggag ttcattttcc 780 cattagcgta caacacttgt acgtctgtgc gccccc 816 // ID HO747755; SV 1; linear; mRNA; EST; FUN; 619 BP. XX AC HO747755; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B10_078 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-619 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; c21418efaf1731914a8262e77f645bef. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..619 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 619 BP; 133 A; 153 C; 202 G; 131 T; 0 other; cttccctcgg gccatcccct gcctgtccgc ctcctcgctc tcaatgtact ctggcggtct 60 actatttcgt atgaggtatg tttattccct tactcgaatc gaagctaaca tggggggaca 120 gcggcgatcc aagtcatccc agcgccgtgg aggaccggcg ttgatggtgc ggaggacggg 180 ggccgacgta gcggctggca gggtgcgact cggagtagga aggatgtcga cggaggactt 240 ctctcaacga cgagggtgag agcgcgacgc cgccaagccc catccagtct tcggggggct 300 tgggttggac ggtcgggtga gggcccgaac gttatctggt atgtaagtca tgttccattg 360 atatattact agttaccctg gcacatcgtc cgttcgcggg taggcggttc tctcgagccg 420 ctggctgcgt gtgcgctgtg cgtacgtgtg ctgctgcaca tctgccgtgc cgccaaccgg 480 gtgtggagga gggggggcga gggcgggtga ggcagtatga cgggaggtat gacgcactgc 540 cagtccaaat gtagtcgagt accataggaa gcaatgaaaa tactattttc gaacgcaaaa 600 aaaaaaaaaa aaaaaaaaa 619 // ID HO747756; SV 1; linear; mRNA; EST; FUN; 675 BP. XX AC HO747756; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B11_093 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-675 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 629360165a99a0fcc13f3fd6b6550cec. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..675 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 675 BP; 133 A; 208 C; 191 G; 143 T; 0 other; gagaaaagac gctctccttt gccccttcca cctggcccgc cagctctacc gctcatcggc 60 aacgttctcg acgtgcccac cgacgggcca atgggctttc gcgacttatg cacaaaatat 120 ggcgatattg tgcacctgga tgtcgctggc caacccatga tcatcctggg atcgcaagag 180 gcagcgtcgg acctcctcga gaagcggtct tcgaactatt ccgatcgagc cccgtccccc 240 atggtagata tttcagggtt ccgctgggca tttaccgtga atggctacgg ctcgttctgg 300 cgtcgaagcc gccgtgcctt ccaccagttc ttcaaccagg ccgccgtgca gacctacgcc 360 gccgaccaac gcctggaggc gcaccgtctg gtgatgcggc tcatcgacaa gccggaggag 420 ttcgtacacc atattaggca tttgttcgga tcctccatca tgcgcgtggt atatggcatc 480 gatattgatg aggagccagt cgattacctg aaaatggcgg aagacacgat gacgatattc 540 tctgaggcgt ttcagcccgg caagtacctc atggagacgc ttccattcct ccgcaacttg 600 ccgtcatgga tgcctggggc gaccgtgaag cgcgagggcg cggcctggcg accggtcgtg 660 cggaagctcg tcgag 675 // ID HO747757; SV 1; linear; mRNA; EST; FUN; 187 BP. XX AC HO747757; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_B12_094 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-187 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 6cf7c14fb3d637d73ebefb0fd7a5201b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..187 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 187 BP; 41 A; 55 C; 51 G; 40 T; 0 other; ctagtggatc caaagctctt catggaccta ggctcgtggg ccgtgggggc ctctcatagc 60 acggcgaccc tgtccccagg gggacctcgc ggtttgtatg ggcttcggct catgcaccgt 120 cccaatattt ccctgtcctg cttcggccct cgggctgggc aggccattaa aaaaaaaaaa 180 aaaaaaa 187 // ID HO747758; SV 1; linear; mRNA; EST; FUN; 438 BP. XX AC HO747758; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_C01_011 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-438 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 438e21a72085ee433f77b4b95fe2931b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..438 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 438 BP; 121 A; 138 C; 106 G; 73 T; 0 other; ttatccaccg catccaccgc caccgcttac gacacaatgg gtttcttcca ctctgagtct 60 gacgaggccc aggcctttga ccaggtcaac aacgcccccc accaggctaa gctctcccac 120 gagctcattg ccgccgctgc atcctacgag gctgccaagg cctacgagga ccacgtcgcg 180 aagaacggcc agcccgacag ccacgccaag gccaaggaga tcctcgcggg cttcgccggt 240 gcgttcatcg accgcatcgt cgagaccaag ggcctcgact acatcgacgc gcaaaaggcc 300 aagcaccact ccagcgagaa gttcgaggag gttgttaccg tcgacaacta ctaaacgacg 360 agcgtcgagt agcttgaagc agatgtatgt atgtacgtat ctatgtgatg cacaatgtaa 420 aaaaaaaaaa aaaaaaaa 438 // ID HO747759; SV 1; linear; mRNA; EST; FUN; 550 BP. XX AC HO747759; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_C02_012 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-550 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 58be5ad5b8b1ca9fe04f469cb25e33ff. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..550 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 550 BP; 112 A; 154 C; 153 G; 131 T; 0 other; cacaatcctt gcaatgttca cgacactgta gtagtagtac aaaacacaaa cgcctaccaa 60 tcgaaacatg ctaacaaaca ggtataatct gaacgagtgt ccgtgctagc ggtgtatgcc 120 cgcacggtgc gggcgacgta actgagtccg tttttctaca atcgtccgtg cgcatgttct 180 ttctctcctg tccttctcgt gctcctgacg cacgttctag acgactgtat gctgaggaga 240 tgtttactcg gtggtgaccg acttcgccag attcctcggg aagtcgacgt cgaagccgtt 300 cttgaccgcg aggtggtaac tgagcagctg catggggatg atgttgagca ggccctgcaa 360 gcagtcaacc gtgcgcggca cgcggatcgt cttgcacccc ttcgggatgc cgtcgtcgtc 420 ctcgttgcac acgacgatcg gctgcgcctt gcgggccgtg atctgcgcat acgcgctctg 480 caccttgggg tatagcgaat cctgcgtcat gacaatgatg acgggcatgt tctcgtcgat 540 cagcgcgaga 550 // ID HO747760; SV 1; linear; mRNA; EST; FUN; 731 BP. XX AC HO747760; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_C03_027 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-731 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 7282335b9414aeecf519b742144f1703. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..731 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 731 BP; 138 A; 216 C; 194 G; 183 T; 0 other; gattgcattc gctcgctgcc gatatcgcct gtgctccagt attctctgcg cgcggaccaa 60 gacgaacacc atgactctcg tgtggctcac acatccggtg ctctggcact cttccagagt 120 gccgtcgtgg aacttcacag caatgactcc cgaccaagcc ttgttgattc aggctcgctg 180 gcacaactgg tacagtgcgg actgggatta cggaaggacg actgtgtact tcttctgcgc 240 ggcaattttc gccttcgggc tcgtcaacgt tgttttccag ctgcggcagc gcgcaaacgg 300 agacgcagtt cgaaaggtcc cgctatacgg caagatcgtc gcggcttcga ggtccatcgg 360 cgcaaggcag atttatatcc ccgttttcga ctactactcc cctcctttgg gtgccatcgt 420 gctcgttgcg ggcttgatta cgttcttcac tgcgttgacg tttgctgtcc atccctaccg 480 ctggcccaac cctgcgatgg gagacagctg gccgatcgcg acgcgatctg gatggatttc 540 gattgggatc atgcccttcc tcttggtgct tggaacgaaa ttcaacctca tcggcatggt 600 gacgggcgtc tctcacgaga aactgcaagt gtaccatcgc tggacggcgt ggattatgta 660 cgtcacgtcc ctgatccaca cattcccgtt catcgtacag tccatcaaga acggcgagat 720 ggtcttgaac t 731 // ID HO747761; SV 1; linear; mRNA; EST; FUN; 766 BP. XX AC HO747761; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_C04_028 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-766 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 7d46c1b069c9086a9ac3840e6749b32f. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..766 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 766 BP; 154 A; 218 C; 211 G; 183 T; 0 other; gctccaagct tgaaaggcca attcggtccg aagggcgccc tcttcagtgg atgggagtcc 60 cgaagacaca atccgagctt cgactggtgt atcatcaagt tagggaccac gggctcaatc 120 agtggctttg atatcgacac tagtcatttc aatggaaacg aggcaccaga agcgtcggtt 180 gacgttctct ccaccccgga tggcacagca ccggaggcca atgaccccag atgggaggag 240 ttactgccca gagtgcaact cgggccgagt tcgagacacc ttttcggggt accgccgtcg 300 aaggccgtca actacgtcaa gctgaatatg tatcccgacg gggggattgc gcgatttcgt 360 gtctacgggt tggtaacacc cgtcttccct gcttacgcct ctgcggtttt tgacctcgcg 420 catgtgtttg ccggcggtcg tgtggtctgc acgtccgacc aacactttgg cgtgggctcg 480 aacttaattc tccctgggag gggtgcgtac gtcgcttcta tccgatgttc cgcagtcgtc 540 acacttgccg taatctcgtc ccttatcatt ttcccttggt aaattgcagg caaagacatg 600 ggtgatggct gggaaacgaa gcgcagtcgt gctcccggac ataaagattg ggcggtcatc 660 cgactgtaag agccgcctct atcgtcattt tccgtacttt ccgtgggtat gctgacctac 720 ttacgatgca accccgcaac ggtcgtcgcc cctcgcatac cacgta 766 // ID HO747762; SV 1; linear; mRNA; EST; FUN; 744 BP. XX AC HO747762; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_C05_043 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-744 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 370117fd1e312256f54d5adf9832a2dd. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..744 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 744 BP; 143 A; 215 C; 208 G; 178 T; 0 other; gggccccctc cgactgcgca tctacaacca ctcccctcta gctatggatg gtcgctttga 60 tccctctatg cctttcatac ccgtcccccc actcccgaac caacctggag tccctgtcat 120 agcgtctgac atatctcacg agaacaagca agccgacgtc gttctcagtc ttccggcgcg 180 acatctgcac atgatgattg tcgccgagat gcctcccatg ccagatattc ttaaggccct 240 gcgggaagat gctctacctg cggggggcga cgctgtggac aagtcgcagg acgcgtccgg 300 tggccgtgga ggcaacctca tgcgagacat cgccggccgc agtcttcctc tgttgttcat 360 ccggtcgtac gatggtgttg gttaccactc gggtcgcgtc atcgcggcgg acaacgttct 420 gcacgccatg gacctctcga accctgcaag gtcaggaggt gaaggtggtg attggctggc 480 ggccgcacag gcggctgcga ggatgtcgga tgacaccaac atgcacgggt ggaacctgaa 540 ggtgttgtag gtcggtgagt tggcacctgg gtacggacgc gtcagaagaa tgtcttacaa 600 gggctactgt acactatact gtcgacgtcg gacgcttcgt cttgctctga tcatgcttaa 660 gtacgtgctt gttttacgtc ggcgatcgct ttctagctac acttgagatc caatgtacca 720 gtgtactctt gaagtatcta tttg 744 // ID HO747763; SV 1; linear; mRNA; EST; FUN; 615 BP. XX AC HO747763; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_C07_059 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-615 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 2a6767a1ef04232960f70e63e3f45660. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..615 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 615 BP; 102 A; 236 C; 142 G; 135 T; 0 other; tttttttttt ttttttttga cacgagacga acgcgaagac gacccgtcct cgctctcttc 60 tgctaccgac cgtccagagg cgtacccaac acacaactac ttacagtacg actaccccaa 120 tcgaccgcca cccacatgcc tctccgcgca ccccgtccga gtttccgagc cccgtcctgc 180 gttccaatcc atttcgcccc ctcgggcagc ccaccctccc atctctgtcc gctcccgtcg 240 cctccccccc tcttccatga tccacccatg acgcccgctc gcagccagga cgccaatata 300 gtcatcttgg ggcacccggg tgccctgccc gtgagagcac tcacggccgc attggcgcca 360 gccacgcctc ggccgcttca aattggtcga tcagctcgcg caggacagcg ccctcggagt 420 gcaactgcct catggccgtt agcgtcacat cccagaaccg gaagccgcgt tcgacttctg 480 ccttggggta tttgacgcct tggaacgtct cctcgcagat gtcgagtgcc atctgcttgg 540 cctctcgtac accctccgcg tttgcgtccc gtacccgctg ccggccattg atgtgcgtga 600 gtgcgtcgag ataga 615 // ID HO747764; SV 1; linear; mRNA; EST; FUN; 470 BP. XX AC HO747764; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_C08_060 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-470 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 8aab1b21ddf478d4ed4b412d40852de2. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..470 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 470 BP; 85 A; 186 C; 128 G; 71 T; 0 other; acagattggt cgccattcaa cgactccgtc gtcgcctccg ccggcgaaga tggcaaggta 60 atgatctgga aggtcgagtc ctccatcttc gaaggctggg ggcaggatgg ctgggagccc 120 caagacttcg accccgtcgc ccgcatcgac gtctccccgc gccgtgtcgg ccacgtcctc 180 ttccacccca cagccaataa cgtccccaca cccgcaccca ccaaaaccac ttccgcgcca 240 ccacccgcgg ctgctgctgc tcccgcaccg gcccccattg ttcgcgaaga gcctgccccg 300 tctcccgtct ctccgccccg cgcagagcct acgcgctcgc tatccacctc gcaggatgac 360 ttgcaggtct ccgcgctcaa ggaggagaac gcgaagctga ccgcggagct gcgcgaggcg 420 cgcgcacaga tccgcaacct cgagctgcag gtcgagaccg tgcgcgcgaa 470 // ID HO747765; SV 1; linear; mRNA; EST; FUN; 398 BP. XX AC HO747765; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_C09_075 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-398 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; cd44db5567adc52cfaac416b0721f232. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..398 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 398 BP; 85 A; 91 C; 112 G; 110 T; 0 other; atatgcgggc ttcttcgtgc atcttaacga tattcctccg gtgttgcgct ggttgcagtg 60 gctttgcccc ctgaagtact ccttggaggc gctctctgtc aacgaagtcg gctcgggcct 120 catgatagaa gacacactcg aaggcgtgcc tgtcaatgtc tcagcggcgc tcattatgca 180 gacgctgttt ggatttgggg cgaacaacta ctacagagac gtgctggtgc tgtttgcgtt 240 cattgccggc ttcgggcttg gggttatatt tgtggtatgg gcgaaggtgc gcgagaggcg 300 gtagccgaac ccctgcttct ggacgttgta acgctagatt gggacatgaa ttaccattca 360 tgctgttgta ttttctcctc aaaaaaaaaa aaaaaaaa 398 // ID HO747766; SV 1; linear; mRNA; EST; FUN; 252 BP. XX AC HO747766; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_C11_091 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-252 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 857bb762db529143d2fb95ba81082b90. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..252 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 252 BP; 44 A; 98 C; 65 G; 45 T; 0 other; tggttcctag cacccctctg ctcttcccgg cactctcttc aacaacgctc ctcgccgaaa 60 tgctcttctc gcagctcacc tttgtcgccg ccctcctcgg cgccgcgtcc gcggcactca 120 tcgggcaagg tcgcatcgcg gaatgcgcgg atgagcaggt cgtcaacacc gcgtacatcg 180 gcgcgaacaa ggacgtcaag gtcacgctct cccactgcgc gaacgaggcc ctcgtcaacg 240 cccacggcga ag 252 // ID HO747767; SV 1; linear; mRNA; EST; FUN; 768 BP. XX AC HO747767; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_C12_092 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-768 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; ce9e3f46cd165f111be7841d665bad1a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..768 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 768 BP; 151 A; 230 C; 239 G; 148 T; 0 other; acttcactcc atacagaatt acaagggcac gcgatcaaga accaggattt gcttgcggcg 60 tcgggcgcag tgtcgctcaa ggggtcagca ggcatgtctg cgctcgccga ggacatggag 120 gcagcggcgc aatcggaaga aggcgtggta ctgcgtctgg cggcgctgga cgagggcctc 180 caggcacgcg accgcgaact gacggagatc gcgaagtcca tcgcacagct agcggagctg 240 ttcaaggacc tgtcggcgct ggtcatcgac caggggaccc tgctggatag cgtggagtac 300 aacatcgagc agaccgcagt gcagatggaa gacgcggtgc gggaactgaa cactgcaacg 360 aggtaccaga agaatactgg gcgccgccag tgcatcttcc tgctgctcct gattatcttc 420 ggcctcatca tcgtcctcat cttcaagccg cggcgacatc actcgccacc cgcactaccc 480 cctgcccagc ctcctcctcc ggccaccact ccaaccgccg tggtcaacgc gctggtgaga 540 gcgtccggat cgctcgggac gcggtcgttg cgtgtggtgg agactccgca cgcgtcggtg 600 cggatgttgc ggcatcggag gggcgtgtat aggtgaccgg gcctgggcga gcacaggtgg 660 cctgcacgga tcatatgatt tattggtttt cggtagcacc cgtattctag atcttcgcta 720 gcattcctac ttaccatccc caaatttcta atcgtagcgg gggctgct 768 // ID HO747768; SV 1; linear; mRNA; EST; FUN; 697 BP. XX AC HO747768; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_D01_009 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-697 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; a6db4c6ad9b32c29d05c96bd50948d14. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..697 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 697 BP; 151 A; 229 C; 191 G; 126 T; 0 other; acctccttcc cctcacatta tatgcttctc tcctcgtctc ccaggacgac gcactccccc 60 catggccgcc cacgtaagac gcacaactgc actcacccgc actctgacgc gttcgctggc 120 gactttgaat accgcgccac cagcggggaa gagtccaaca gcgattgtga tgctgaacat 180 gggcggtccc tccacggtgt cggaaacaca tgacttcctc aagaacctct tcttggacgg 240 agacctcatc ccccttcctt tccaatccgt tctcgcaccc ttgatcgcga ggcgtcgcac 300 cccccaaatc gagaagcagt atggggacat tggcgggggc tcaccgatcc tgaagtacac 360 caagatacag ggggaacgta tggccgccct cctcgacgaa ctacacccta cgacggcacc 420 ccataaggca tacgtcgcgt tccgctacgc gcggcccctc accgacgaga ccgcgcgcca 480 aatgaaggct gacggcgtca agcgtgccgt cgcgtttacg caataccccc agtatagttg 540 cagcacgaca gggagcagtc tcaacgagct gtacaggcgg ggcaaggcgg gagaggttgg 600 ggatgaggta gagtggagtg tgattgaccg gtggggtacc catcctggct ttatcgaggc 660 ggtcgcacag aacatcgagc gagcgctcgc gaagttc 697 // ID HO747769; SV 1; linear; mRNA; EST; FUN; 458 BP. XX AC HO747769; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_D02_010 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-458 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; a075eda1463f5810a18ae935695451fd. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..458 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 458 BP; 84 A; 110 C; 166 G; 98 T; 0 other; cgaagcccga gaacctcacc gcggccgggc tgccgaaccc gtggagcacg gaccccggct 60 tcgagtacgc gaacgggaca gaggtgatcc agccgccgac ggcgacgagc acgaagccgg 120 cgggcgggaa gaacgccgct gcgcgtgcgg cggttggcgt gagggagggc gcggtggtgc 180 tggttggggt tgtgcttggc gcggttgtgt tgctctgaga tgggataggg ctgtgtggat 240 tcggggtggt cgccgcgcac gaacatcttg cagtacttcg tcgagtgcat tagatatttc 300 tctgactgtc tgggatgttg aaatctgagc gccgagcgta gagacccgca atgaaatgtg 360 ggagtttttg accgccaaga gttcgcgatg ctcttgatgg tagcttccta agtacatgta 420 cagttgtaca gttgtacagt tgcaaggttg ctcgactc 458 // ID HO747770; SV 1; linear; mRNA; EST; FUN; 765 BP. XX AC HO747770; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_D03_025 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-765 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 08ea11a7a3ebac5934c943d7fbea9c8a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..765 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 765 BP; 141 A; 281 C; 210 G; 133 T; 0 other; caccatcgga gacaccgagg gtgaggaggt tgcttggtgc accaagaagg gccgtggtac 60 acgtatcatc cccgctggcg ccatcaccgg tgtccagttc atgaagaccc ctggctacct 120 ccagatcgtc ggcttcatcg accagaccaa gatcaacctc cagtctgatg atggcggagg 180 agagctcgac ccccacggtg ctgatctccg tggtaaccct cttggcggtc tcgtctactc 240 gaacgggttc gcgagcaaca acgggaacaa caacacgttc cagcaggtga tcgagtggca 300 caacttcatg ggctcgaact cgttctgctt caaggtctgc gaccccgctg gccccaacgc 360 accccagctc tgccagcaca tcttcgatct tattggtgtc gcatacaact gccccaacgc 420 cgcccagaac ggcacgttcg agtactgcga gggtgagaac caggaccccc ctggtgtcta 480 cacctcgaac ggccaggtcg tgacctacac ccagccccct gagtcgctcg gcccgatctc 540 gacgatgccc tacgacccaa agatccccgc gtcctcgaac tgcgtccagc tccagtccgc 600 cgccctcttc accgacctcc ccgcccccac cggcgcgtcg accgctgcta gcggttcggc 660 gactgccacc ggctcgcgcg ctggctctgc tggtgctagc ggctccgcta cccgcacggg 720 ctccggctcg acggcgagcg ccactggggc gactggcaac ggtgc 765 // ID HO747771; SV 1; linear; mRNA; EST; FUN; 598 BP. XX AC HO747771; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_D04_026 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-598 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; dab0e3474a25004be0e9d7d02716aa06. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..598 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 598 BP; 143 A; 169 C; 153 G; 133 T; 0 other; ggcgagctct tgaaggggta tgttagggag gcacaacggc tggctcctca gttcgcggcc 60 gttcttcgcg aggatgctgc ctctgcaagc gcgtcgagtt cccagccgaa gcagaccttg 120 gtgctggact tgaagacagc gcttgtgaac ccgggcgatt tccccgaccc caagaccgtc 180 aaccctcgcc gcccggtgca gtcctatgac atcctgcagg aggcaagctt ctacaaatcc 240 cttggaagct ctcgcggtga agagatcgtc gtcgagatcg tcaaaagtgt cttccaactc 300 ccaaacctcc agcacgcgaa gggcgctgcc ggcaccatca gcaggctcga gacgcagaag 360 ttcggcgtcc cgttcgacat gtacaccgat aacattggcc tgccgagcta ctggcctacc 420 tccatgctcg tcacgtacgg agcgtaattc gacatgaatc tgatgaccca tgtcttgaat 480 gaatcttgat gctgcatgca cgatatacgt tgaatgattg gctacgttct tggctttgta 540 catcatcatt ccgcaggaat cgatttttgt ctacgttcaa aaaaaaaaaa aaaaaaaa 598 // ID HO747772; SV 1; linear; mRNA; EST; FUN; 450 BP. XX AC HO747772; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_D05_041 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-450 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 7413149fa728c9a35f230d3e0742de19. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..450 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 450 BP; 91 A; 137 C; 128 G; 94 T; 0 other; aaagagctgt tccgctggca gcccattgtg cgcctcgacc ttcctcgtaa gctgcgggag 60 gacgacgtct acaatggcta ctacttacag aaggactccc tcgtcatcgt caatatctgg 120 tcgatcctgc atgacgagaa ggtgtactca aacccgcaca ccttcgcacc cgaccgcttc 180 ctcaagaacg gcgcgctcga cgccgatatc ctcgaccccc tagacgtcgc gttcgggttc 240 gggcgccgca tatgtcctgg gaggtacatg gcgtacgaca ccatgtggat cgcgatcgcg 300 acgctcctga cgtgcgccga gatctgccct gcgaaggata gtgaaggaaa ggatattgag 360 gtcaaggagg actacgctag tgcgtttgtc acggagccgc ggccgttccc ttgtcatgtc 420 cgtttccggt cggaagcaca cgctgcgtta 450 // ID HO747773; SV 1; linear; mRNA; EST; FUN; 714 BP. XX AC HO747773; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_D06_042 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-714 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; bf9e40d3c467c1301a41d2cde5f2eddc. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..714 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 714 BP; 157 A; 197 C; 198 G; 162 T; 0 other; tttttttttt tttttttttc aaattgactc cgtgcacgag ttggctcaaa cacggcgggt 60 ttattggaca acgctaacgc acttgacatt cacaacgaca aagcaacaac aactctacga 120 cattcgggac actcagatac atcgccatcc tggctactgg caagccacag ttctacggga 180 agtgtttgat agctgttcaa tggttaacaa gaacgagagc ttcaagcggt cttccacagg 240 aggtacaaat agctcttgcc gcggcctttg ttgatgtcga tagtgtttcc agcccacccc 300 gcaggacgct cggtcatcgc attgtcaaga cgcagcagag caacctcagt aaccctctcc 360 gtctgcgtgg cgctcttctc gagaaggagg tagcggtagt cgccacccgc acccttggcg 420 aggtcgctca gcgagccgtc ggcgctccct tggatggcga tgtcgaaacc agtgaccgcg 480 gcgtcgcggt cggtcgtgaa gacagggacg agccagacgt attttccgcc aaactggtag 540 ttgatgtcgt cgttctggtg gttggtctcg aggacgcggc cagagggagc ttgggagggc 600 tcgtcgccgt agacgacctc gatgttggag ataaactgca tgattcgtga ggaccacgat 660 gagcacaccc tttggcgagt cgacgaaccg ccatctcaac gtccccgcca cagg 714 // ID HO747774; SV 1; linear; mRNA; EST; FUN; 770 BP. XX AC HO747774; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_D07_057 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-770 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; f01870179dca269578d7896ed52f9b11. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..770 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 770 BP; 114 A; 253 C; 246 G; 157 T; 0 other; cccatctcga cgtttcgctg atgaaggtcc cgctcctcct tgcgcatgtg gtgtgcacat 60 acgagggtat gacgcctccg aacacgggaa cgcagaccac cccaacgaac acccgtccgt 120 cgctggctgc aaccgcgctt gaggttttgg ccgcatgcgc cacgctggca tccaaggccc 180 ttctctgtgg ggtcgcgctc gccgaagtcg cagcgcttct cgcacaccag cacccatccg 240 ccttgtcgag cgccgccctc gacctcctct tcccccacag tccggccacc gtatccgctc 300 tgcgcctcac ccccgcttcc gctgcgggat gcgtgcttgg gattgcgggg ggccttggtc 360 gcatatggtg tcaccgcgcg ctcggccgct tctacgtctg ggacatggtc atctgcgacc 420 gccacaagct ggtcaccacc ggaccgtacg cggtcgtccg ccacccaggc tacaccgcgt 480 tcgtcgtgat gctctgcggg aacattctgc tgctcgcgag cgcggggtcg tacttcacgg 540 cagcagggct gtgggactcc atgttgtgga agacgacgac gtgcttggtg gtcgggcatt 600 tggtgtgggt cggggcgagc ctgtgctgga ggacggcgga cgaggatgcg atgctgaggg 660 gcgagttcgg cgagagatgg gaggagtggg ccaagaggac gcggtatcgc cttgtcccgt 720 tcgtgtactg atatctcgcg ccatattatc tgccgcctac ttgtcgacga 770 // ID HO747775; SV 1; linear; mRNA; EST; FUN; 796 BP. XX AC HO747775; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_D09_073 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-796 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 097a38fd2a0b8eeb7149180aefb6910f. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..796 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 796 BP; 144 A; 311 C; 160 G; 181 T; 0 other; ggtgttctct tccagtcgtc cgtcgccgcc gctgctcccg ggtggaggcc tcgctcggct 60 ttcctctcac tttctctcta gcagccatcc atccccatgg ccccgtcgac caacactccc 120 cccggcctct ccgaggcaac acgaaactcc acctcccact ggcaagacga ccttcaggcc 180 ctcttcgatc actcaaagga ccgcttcgca gatgtcgtat gggagctcca gtccgaaggc 240 gaaagcgaaa aagcggcgga agaggtctgg ggtcacaaag ccgtggtcta tgctcgcgcc 300 ccgccgtcgt ttcaggcccg ctacttttcc ttcaagcccc ctcctccatc ctctcccatc 360 ccgtattcgg cctctcccac tggcaccctc cctgctcagt ctgcactctc cttgaatctt 420 ggccccgact attcctctcc ctcccgttcc ccttctccct ctccctttcg tgcaacatcg 480 ccctcccctt ccaccaacaa ccctggcgcc ctcctccgtc tcccaacaaa tatcaacccc 540 gccctcttct ccaaggagtt agagtacctc tacaccggaa agggcctcgg cgagtatttc 600 gagttcctct tcgacgccgc agagaactca gaagagggcg atggggacga agccaggata 660 gacaagctcc gcaaagatct cgtcttcatg tggagatcac gcctctactc ggacgttcgc 720 atctctctca caggctcatt ctcctccact aaccatgaaa gctcgactgc catcttttcc 780 tcccatcgct tcattc 796 // ID HO747776; SV 1; linear; mRNA; EST; FUN; 442 BP. XX AC HO747776; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_D10_074 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-442 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 44ea9593a9ce61e0ed80d46682544f2b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..442 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 442 BP; 125 A; 122 C; 110 G; 85 T; 0 other; tccagcgcgc ggttgtagga aacccagcct gcaggcacgg ccgccccgcc tccaaagctc 60 cggtgaagag cactggagag cggcccgcca tacactttgc agttcgctcg tcaacgcata 120 tctccataca atgaactaag cagccattgc gctttggtct gcaatgcata taactgacta 180 acccgatatc gggaccgcgg caaatccggc cgccgagcgt cgtgaagatg acgctcgcag 240 cttgatttcg agcatccgaa aatcaaatat cccaggacat ccttccgcga cccgcgcgct 300 gtgcgtgtgc ggccgcgacg ccattctcaa gctcaagaag ccgtggatag gtagtgtatt 360 tatacgtaga cgtaacgctc gacgcgtgaa ttgaatatat agtctggttt ggcgaaaaaa 420 aaaaaaaaaa aaaaaaaaaa aa 442 // ID HO747777; SV 1; linear; mRNA; EST; FUN; 746 BP. XX AC HO747777; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_D11_089 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-746 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 19913550a0e67cad37f561474a6a278a. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..746 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 746 BP; 177 A; 194 C; 227 G; 148 T; 0 other; ttcggaaggg tatggaagtc aaagttctgg gcgagggcta ctcgccagaa gacgaggagg 60 acatggcgaa ggctgttgta gagcacatct ggattagtgg agcccgatac ttcatccccg 120 cagaagaggt cccagcagga aaccttgttc tgcttggcgg cgttgacgcg tctatctcca 180 agacggcgac aatcgcttcg ctcgacgtcg aggatgacct tcacatcttc cgcccgatca 240 agcatatgac gcagtccgtt ctcaagattg ccattgagcc tatcgcgccg tccgaactgc 300 caaagatgct agctgggctt cgcagtatca acaagtcgta ccccctcgtt gcgaccaagg 360 tcgaggagag cggcgaacac gtcgtcattg gcacgggcga gttgtacctc gactgcgtca 420 tgcacgatct gcggagactg ttctcagaga ttgagatcaa ggtgtcggac cccgtcacga 480 agttcgcgga aacggtgctt gagacgagcg cgctcaagtg ttatgccgat acacccaata 540 agaaaaaccg tctgacaatg atcgcagagc cactagagcg tggtattgcg gaggacgtcg 600 agactgggcg ggtgaacata cgcatgacgg cgaaggagcg cggcaagttc ttcgaggaga 660 agtatcagtg ggacttgctg gcgtcgcggt caatatgggc gtttggccca gatgacagcg 720 gccccaacat attgctcgac gacacg 746 // ID HO747778; SV 1; linear; mRNA; EST; FUN; 679 BP. XX AC HO747778; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_D12_090 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-679 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 352ee88cf337c06ef931e2e26bd1b0c4. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..679 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 679 BP; 179 A; 172 C; 164 G; 164 T; 0 other; catcagtgcc gacgccatca taaccagcac catcgattcg gtcaccgcca tgtacatcga 60 ggttgtttcc caaggcgaca tcatctctcc attcgcgacg aggtacccct tctacttacg 120 ccttaagact gggatcgcgc cggcatacgt ccacctcaat ttcacgaaga gctggggaga 180 cgcaattggg accaactacc tgcaggtcgt accggaaatg gagctggtca ccattggctt 240 caacgatatc ctatctttct acaaggaaga actggcaggg gagacggaca actatattca 300 catgcgcgca agcgcggaac aaaaaccggc ggctgccgtc ctccacgaac tcgtcgagga 360 gaaccttgag tcgtggttca agatcaagca gctcgcatcc cttcaatccg gcctggtaga 420 aatctgtatt ggttatctca tgggttatgt tgagttccac ttcaaagcac gccgctatcg 480 cctacacgag atcgtgggcc ggtcatgaac gttaacgttt acgttcagcc tgagtgtttc 540 atttgatggg ggtctcatga agtagtttcg tgctttgcgc tgtggaatga tcgttcgcgc 600 tgtatcgtcg gcattatgat gtaaccttaa gtatagatga gcttttggat ctcaaaaaaa 660 aaaaaaaaaa aaaaaaaaa 679 // ID HO747779; SV 1; linear; mRNA; EST; FUN; 789 BP. XX AC HO747779; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_E01_007 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-789 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 347ea5afbc93dc06df67119b33e09b05. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..789 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 789 BP; 183 A; 233 C; 205 G; 168 T; 0 other; ggctttcgaa cactttcttt tgcattcaca tcaaaaatgg gaaaggagaa gacccacgtt 60 aacgtcgtcg tcatcggtca cgtcgattcc ggcaaatcca cgactaccgg acacttgatc 120 tacaagtgcg gtggtatcga caagcgtacc atcgagaagt tcgaaaagga ggctgctgag 180 ctcggcaagg gctccttcaa gtatgcctgg gtgctcgaca agcttaaggc cgagcgtgag 240 cgtggtatca ccatcgatat cgccctctgg aagttcgaga cccccaagta catggtcacc 300 gtcatcgacg cccccggtca ccgtgacttc atcaagaaca tgatcactgg tacctcgcag 360 gctgactgtg ctatcctcat catcgctgcc ggtaccggtg agttcgaggc tggtatctcc 420 aaggatggcc agacccgcga gcacgccctc cttgccttca ccttgggtgt caggcagctc 480 atcgttgccg ttaacaagat ggacaccaca aagtggtccg aggaccgttt caacgaaatc 540 atcaaggaga cgtccacctt catcaagaag gtcggttaca acccgaaggc ggttgcgttc 600 gtccccattt ctggctggca cggcgacaac atgcttgagg agtccaccaa catgccctgg 660 tacaagggtt ggactaagga gaccaagagc ggggttgtca agggcaagac cctattggac 720 gctatcgatg ccatcgagcc ccccgttcgt ccctccgaca agccccttcg tcttcccctc 780 caggatgtt 789 // ID HO747780; SV 1; linear; mRNA; EST; FUN; 774 BP. XX AC HO747780; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_E03_023 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-774 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; d2b8a6e5d7c5b2b9087ea8c01f189d07. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..774 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 774 BP; 168 A; 258 C; 210 G; 138 T; 0 other; cacacatctc accaacatca ctggcaacaa gggtcgttct tggagcccgt tcaagcaact 60 gcgcaaaacg ttccacccat cctcctcctc accagcacta ccaatggcag agtaccgtca 120 gctcggaaag tctggcctcc gtgtgtcggt ccccatctac ggtggcatgt ccgtcggtaa 180 cacgacctgg agcccctggg tccttcccga agaggagtct ctcaccgtcc tcaaggccgc 240 gtgggaccag gggatcaaca ccttcgacac ggccaacctc tactctaacg gcgactccga 300 gcgaatcctc gggaagttca tcaagcagaa caacatcccg cgcgagaacg tcgtgatcct 360 caccaaggca tttttcctcg tgcacaagga cgaccctaca tgcgtgaccg tcctccggcc 420 cgacctcaac acgacacgcg actatgtgaa ccagggtggg ctctcacgcg cggcgctctt 480 caaccaggtc gaggcatccc tgaagcgcct tgacacgccg tacatcgatg tgctgcaggt 540 gcacacgttc gacccgacga cgccgcctga ggagacgatg cgcgcactgc acgacctcgt 600 gcagagcggg aaggtgcgct acatcggcgc gtgcaacatg cgcgcgtggc agtttgcgga 660 gttgaatcac gtggcggagc tcaaccgctg gacgacgttc acgagcattc aggtcgagta 720 ctcgctcctg taccggcctg aggagttgga gatgatcgcg tacgcaaact accg 774 // ID HO747781; SV 1; linear; mRNA; EST; FUN; 643 BP. XX AC HO747781; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_E04_024 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-643 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 510586442d932a0efe9cb56f079c04ae. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..643 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 643 BP; 181 A; 168 C; 195 G; 99 T; 0 other; gaggagctgg acgacgggcc cgagccggtg aggcagaagc tgaggaagga gtgggtggag 60 gatccaactg aagcacggaa gacccttcaa agtatctttg atggagtcgt tcgcgagcgt 120 ctgaaagagg tcctgcggca gtatgagctc agagaacgac actttgagtg cgtcgtgcga 180 tcgaaggagc tcgaggtcct cctgtcgcgc gcccgcgcgg cggaacagaa agagctcgcc 240 gacgccgaaa aggctcgagc ggacaaaacc gaggacgaga gcaaacagct acggaaagag 300 ctagaagacg cgacagagca acaaaccatc ctcattaaca aactactgtc gtgttgtaac 360 gagcccgata gcgaacgaac gcgacagttc ctccaatcat tacacggcgc cctgcatccc 420 aagaacgaag ctcacaagca cggcactcca ggggaggggc agaatcagaa ggcgaataat 480 gtcaatgcca gccgtaaggc agatggctcg tgacggcgac gtgggcgagc gcgcggtcgc 540 cggtccagct ctttacgtgt agcctgtacc tcgttgcacg ctcagcacac atcgtcgttt 600 tctggtaccg agattcgcgg cttcaaaaaa aaaaaaaaaa aaa 643 // ID HO747782; SV 1; linear; mRNA; EST; FUN; 376 BP. XX AC HO747782; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_E05_039 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-376 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 5ff1ac677f87f1a84a8da06725a9749f. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..376 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 376 BP; 83 A; 120 C; 53 G; 120 T; 0 other; tcatcttttc cattcccttg acgttgtttg agtatacctt atgcattatg tttcccccca 60 tctgtttctc atctctcaac cagtgccacc gcattttttt ccgtggcctt cgctactccg 120 ttctcgtttt ccaccattcc ccacccactt gcatttggag tagcgtcgta catccccact 180 tcccccatgc tccccatcgt cgtccataaa tgcaaaaccc atcgctaatg ttcagtcggc 240 gttatagtac ccgactccca ttcacttcca ccatactccc cttatctcgc atgtctctcg 300 ccatagtcgt cgtcgtcttc gtggacgcaa tgaattaaat gtgactgttg tatattcgaa 360 aaaaaaaaaa aaaaaa 376 // ID HO747783; SV 1; linear; mRNA; EST; FUN; 725 BP. XX AC HO747783; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_E06_040 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-725 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 76bf68a179368d4b57dec807a88b637b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..725 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 725 BP; 162 A; 245 C; 162 G; 156 T; 0 other; tccagtcgtt caaaaacgcc ctccgacgat ggcgtccagc agcaagctct ctcgtggaca 60 aatcgcatcc gctcaaatta acggagacta tgccatcaaa cctgaaagct ctcagcctgt 120 gcttgacacc tccaactggc ctcttctcct caaaaactac gacaagctcc ttgtccgctc 180 agctcacttt gtccccatca tcgccggatg caacccgctg aagcgggata tcacatccta 240 cgtgaaatct ggggttatta acctcgacaa gccatcaaat ccttcgtccc acgaagtcgt 300 cgcatggctt cgaaggatat tgcgggtgga aaagacgggt cacagcggaa ctcttgaccc 360 taaggtcacg ggctgcctga tcgtatgtat tgaccgcgcg acacgtctgg tcaagtctca 420 gcagggtgca ggaaaggagt acgttgccgt ccttcgcctc cactctgctc tccccaaccc 480 caccgccctc cctcgcgcca tccaaacact caccggcgcg ctcttccaac gaccacctct 540 catctccgcc gtcaagcgtc agctccgtat tcgtacaatc tacgaatcaa aactgctcga 600 gttcgacgaa aatcgcaacc tcgctgtgtt ctgggtctca tgcgaagccg gcacatacat 660 tcggacactc tgcgtgcatc tcggtctcct gctcggcgtc ggtggccaca tgcaggagct 720 ccgga 725 // ID HO747784; SV 1; linear; mRNA; EST; FUN; 814 BP. XX AC HO747784; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_E07_055 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-814 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 566abbf0bb88e0474408f5b0d1a76919. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..814 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 814 BP; 152 A; 269 C; 234 G; 159 T; 0 other; ggtatcacgt ccggccatcg aaggtcgagg tcgacgcctg ggccagcctt gttgacggcg 60 gtgctgacaa gtggagctgg gacaagatgt tcgcggcgat gaaggcgagc gaaacgttca 120 cgccgccgtc gtccgacgtg cagaccgagg gcggcatcca gtacgttgcc tcgagccgcg 180 gcacagatgg tcccatccac gtctcgtatc ctggcttcat gcttcccgtc gttgggaact 240 ggacgaccac cctggcaaac gtcggcgtgg atgtctccgc tgacgcgtac gccggagatg 300 cttggggcgc attcgtcgcg agttcttcga ttaatcccag caactggacg cggtcatacg 360 cccggtcggg atacatcgac ccccttcccc ctcgctaatc tcgccatcct cccgaacgcg 420 atcgccaccc gcatcatctt cgacacctcg aacgcgagca acctcactgc caccggcgtc 480 gagtggactt ccgcgtccgg cgctgcgaaa cagaccatca aggcgaagaa ggaagtcatc 540 ctggccgctg gtgttgtcgg tagcccccag ttgttgcagc ttagtggtgt cggaccgtcc 600 gacgtcttga aggccgctgg ggttaacgtg ctctcgaacc tgccgggtgt tggacagcac 660 ttgcaggacc accttagcac cgcagtgact tggacgacca agattgatac cacaggctct 720 ctgcacgcca acagtgcgga tgccagcaac cctgccttca tgtcccttgt caactccgcc 780 accgcatacg tcaacgtcac tgatctcctc ggcg 814 // ID HO747785; SV 1; linear; mRNA; EST; FUN; 645 BP. XX AC HO747785; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_E09_071 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-645 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 750e96920048be527e6481e78c84aef9. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..645 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 645 BP; 151 A; 159 C; 200 G; 135 T; 0 other; tttttttttt ttttttttgt tcctgcactt gcacgcatat atacttggaa gcccagcgta 60 gttaaacata tctgacggcg agctatggcc gacctggaga gtgcgcagcg ggactagagc 120 catgaatcac agaaagtgaa gaagctattc agcccggaac catctcgtgc aatcctggat 180 tgaggagtcg gccgaaggtg tgcggggatg cggttttaaa acgagttctt cagcatatcg 240 tacagtaggt cgctgtacag aaagacaagg ctcttgttaa cgatacaacc accatcttcc 300 cattctactg aatgaaaatg gtctcgctcg ggtcgtcgag ggacgggaag aacagtgccc 360 cgacgaggac gaagtgaggc acagacagga gcgcgggtcc agacgggaag tcgcggggga 420 tcgtgacggt atagttctgg acgagcgtcg tgcttccggg caccgcctgc ggcgcatacg 480 gcccagcgaa gagcacgatg cctagctgct gggtgggatc gatgccgtca caggtaccag 540 ggggtgccct tcccgcacag gacaggaggc cgatcgcgac cgagacatcc tgcgagttgg 600 tgagagtatc aggcttgtta acctggacca cgaactgatc gccgg 645 // ID HO747786; SV 1; linear; mRNA; EST; FUN; 721 BP. XX AC HO747786; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_E10_072 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-721 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 03e47576b48373482f7a1dfd454d3920. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..721 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 721 BP; 150 A; 246 C; 186 G; 139 T; 0 other; atcccgacta cctcctcccc atctcttcag gtgtacacct gcagcgggta tccagaatat 60 cgcctaggcc tcccaagtcc tcagtcgacg tccgctctcc tacccccctc tctgtactct 120 acggctagct tagcacgaac atctcctcga accctctcgc catgttcagc tcaacctcac 180 gccatcttcc ccccggcccc cgcggcatgc ctattgtcgg caacctgttc gatattccct 240 ccacggagca gtggatttac ttcaagcagc aggcagacat gctcgggaag ccgctcacga 300 cgtacaaagt gttcaaccag acgctcatcg tgctgaactc gcgcgagtcc atcgaggcgt 360 tgttcgtgca gaagcaggac gtgtacgcga gcaagccccc gcgcaagatg tcagagatct 420 cggacctgac aatgacgctg ccgttcatgg atccgggtca ggtgttctcg aacgcgcggg 480 agcagttcca ccacggcatc gggaagaccg aggtcgggac gtacaaccgg gactacgagg 540 cggcatcgag gagcttcatg caaactttgc tgggcgacct gcagtgcacg aagctcgaca 600 aggcgatcga caactctctc gggcatatct tcctcaaggt gtcgaccgga tacgacggcg 660 agcagaccgc gcccgtcctc gagcggatga acaacctcgc gcactttgcc gccgacgtcc 720 t 721 // ID HO747787; SV 1; linear; mRNA; EST; FUN; 574 BP. XX AC HO747787; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_E11_087 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-574 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 4f203f7db74d2b4f51178956347a7e98. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..574 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 574 BP; 98 A; 204 C; 191 G; 81 T; 0 other; ggtctgcacc tctccttccc tccatccgcc acttcccgta tccacaaaac ctcatcctct 60 ccgaaatgca cgcacgggcc atccttctca gcctcgctct tctgctctcg agcagcgccg 120 tcctcgccca aggccccgtc cagacaacct cggacgtctc ggcgacggac tctgcgacga 180 gctcagagcc cgcgactggc acagagtccg caacggcgac ggacaccgcg acagacaccg 240 acaccgagac ggcgaccgag acggccacgg acatcagcgc caccgcgacc gcgacctccc 300 ccgcctcggg cacaggcaca gcgacgagca agcccgctac gtcctcgtcg ggcagcagcg 360 cgagcgggtc gcgctctgcg agtgggacag gcgcaggcac ggggaccgcg acgggctcca 420 gcgcgtcggc gagcgggacg ggtaggccga atggcgctgc ggcgctttcg agcgggctgg 480 agggcgcgat cgcgctcgtt ggggggatcg tcgtctctgg gttgatgctg tgagggggct 540 gttgcggatg tacctaatag ggatggagag gggc 574 // ID HO747788; SV 1; linear; mRNA; EST; FUN; 727 BP. XX AC HO747788; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_E12_088 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-727 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 46234af73f1a42e31bd0d39da4d58238. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..727 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 727 BP; 146 A; 233 C; 174 G; 174 T; 0 other; tttgtacctt gtgacctcca gttcggtagc tagtcgacag agcttcgcct tcgactcctt 60 gaaactggtt actctgctcg ctcgctgaaa ggaaaccacc ggctgctggc atgtgtgagc 120 acgagatagt aggggattgg taccgaggct gtcaggtata ctttctcccg actttccccg 180 ctcatgaccc gctcatgata tcctacgcaa gcactttcat ggtcgctact atacgggcga 240 gcgcgttgat tgcggctccc aacggtgcaa gacgagctcc gcgcacattc atggcgcgac 300 cacggtctgc cgctgcgaag ctgtatgtat gcgctcactc acctgcgctc ccgacgctat 360 cctctccgcg ttccgcgtct aacgccgctt catgtgctgt aggtggtgag ggacaagaag 420 acagtgcaaa acatgatcca ggcgaaacat cccgactgca agtgacgagc gcccacgttc 480 ttgcgggcgc tccgagaggc gcttacggat cgcgcctgac acgtcattcg ctactcgacg 540 cccggataac tctttgccat ccaccctcac cgtcaccact ttccactcgc attggcatac 600 cacgactcga ctggccctct gcagctctcg tacttggact tcgctatccc accctgtcaa 660 acttagatgt atcatagtca ctcgcctgta cctttgtacc taataatgcg ccactatatg 720 ctatcga 727 // ID HO747789; SV 1; linear; mRNA; EST; FUN; 691 BP. XX AC HO747789; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_F01_005 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-691 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 225bb7251604faefe8659fb6fe61d900. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..691 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 691 BP; 166 A; 214 C; 177 G; 134 T; 0 other; gacttctact cctaccgcac ctcccaggca ttcgtcctaa caccacatcc acaactcccg 60 ccatgcagtt gttcacgatc gtcgcctccg ctctgaccct cgcctcggcg gtcgtcgcta 120 tgcccgccga gctccgcacc cgcgcgaaca ccgtgcaggt tacctgggac tctgtctacg 180 acaatgggtc gaactccttg aacatcgtct cgtgctccaa cggagccaac gggcttgagc 240 cgaaatacca cacgttcggt ggcctccccg acttcccctt catcggcggc gcacaggcca 300 tcgcaggatg gaactcgccc aactgcggaa cgtgctggca gctgacgtac aacggcacga 360 cgatcaacgt gctcgcgatc gaccacgccg actccggctt caacatcgct aagaccgcga 420 tgaacgcgct cacacacggc caggctgacc agctcggcgt cgtgcaggtg acgtcgaagc 480 aggtcgacaa gagcgtgtgc ggcctgtaat cgggagagca tactagcgat accgaataac 540 tttggactcg gaatggactt tcggatggaa cagtgcatgt acgatatgcg attctggtgg 600 actatgtgct gtactatgtg ctgtatcatg gcttatcggg attgaaatgg cactgacata 660 tgagctcaaa aaaaaaaaaa aaaaaaaaaa a 691 // ID HO747790; SV 1; linear; mRNA; EST; FUN; 663 BP. XX AC HO747790; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_F02_006 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-663 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; f26030b1936d0605cf10c850abb834ea. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..663 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 663 BP; 139 A; 175 C; 201 G; 148 T; 0 other; ggcctcatct cgtccatcga tcccggcgtc gccatcacgg ttcttcacca agcacttgat 60 gtccagacga cgtacggtgc tgtcatgctg tccttcctcg gtgccttgca ctggggattc 120 gagttcgccg gatatggcgg acacaagtat tacccgcgcc ttctgttggg agcagctccg 180 gttgtgtatg ggtggtctac gctggctttg gatcccatgg gcgcgctgat tgctcagtgg 240 gtcggtttta ccacgatgtg gtgggctgac ctgcgggcca ccacggcagg gtggacaccg 300 aaatggtact cccagtaccg gttctatctg tctgtcatgg taggattctg tatcatcggc 360 tcccttatgg ccacaagcat ctgggggcct gttggtggcc acggtctcat cagccacgat 420 ctcaacatga ttcgcgctga gcgcaagagg agagcacctg agagagaggg atccgtccct 480 ggggagattg aggctgtcgc ggcaccagag gactccgacc actatgtcat cgtgaggaag 540 cacgagaatg gcgggggcga aggcaatggc aatggccagc agcaacagtg aacaaccccg 600 agcttgagaa gtattgaaat aaagtgcgaa gcacgctgta ttcgattatg tacaacaaac 660 ata 663 // ID HO747791; SV 1; linear; mRNA; EST; FUN; 814 BP. XX AC HO747791; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_F04_022 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-814 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; b661dddba174626906fb04fe971ef79d. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..814 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 814 BP; 176 A; 290 C; 217 G; 131 T; 0 other; atcgacacct gtcatacaca acatgcactt cgtctcgacc gtgaccatcg tactctctgt 60 cctcgccgcc gaggttgtcg gcaggcagat gaccgtcgtc aacaactgtg cctataccat 120 ctggcccggt ctgtacaccg acccgtcgag cccttcgaag cccacccaag cgaccggctg 180 gcagcaggac gcgcacaaga cggtctcgtt ctccgtcccc gacaactgga agtccggtcg 240 catctggggg cgcaccgggt gtgacttcag caagaacgct cccggcccga accagtgcgt 300 taccggaggc tgcaatggcg gcctgaagtg cgatcccaag acgggcacgg gtgtcccccc 360 ggtgaccctc gccgagttta cgttggcatt caacggccag ccggacaact acgacgtatc 420 tgtcgtcgat ggtttcaaca ttcccatgtc aatcaccaac aacaagggct gccatgaggc 480 cagctgcaag gccgacctca acccgaactg cccagcacag ctgaagggcc ccaccgacag 540 ctctggaaag gtcgcgggct gccagagtgc ctgcaaggcc ggcctcggcg acaaaaccaa 600 caacccgaac tgctgcaccg gctcgcacaa cacggccgcg acctgcccgg cgtccggcgt 660 gcagtactac agctacttca agaacgcatg caaggactct tacgtcttcg catacgacga 720 gcccagcggc actgcgctct ggacgtgcga cgcgaacaag gcggctgact acaccatcac 780 cttctgcccc tagatcgtcc gaaccacgcc cagc 814 // ID HO747792; SV 1; linear; mRNA; EST; FUN; 723 BP. XX AC HO747792; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_F05_037 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-723 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 655e51ef9bc595a35b09dd671961b9f3. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..723 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 723 BP; 163 A; 186 C; 220 G; 154 T; 0 other; tttttttttt tttttttttt ttttttcagt gcgccaccgc ctacgaagag atatcatggg 60 ccgtggcggt aggtaacatc acagtcctat atacaagtga atatatgcta ctttacattc 120 cgcagataga ccacagcatt cctcgcgtcc agcatacagt cgcaacaaac cacgacaaag 180 gagatacagc gcaggggctc gaatatctcc gaacggccga gtggggacta ggccgcctcg 240 agcgacttgg tccacgcctt gattcgctcc cagcatttgc gcacctcctt cggccacgct 300 cgttccacgg agacgaatat gaggaagtcg tggctgaaac gttccggtat ctcatcggct 360 gcgtaagagg tggccgacgc gccgagttgc gccaccaggg cggtgattga cgggacgaat 420 cgctcggtct tgccgtagta caccagcgtc gggggccact gggggagggg cggatggagc 480 gcaggagaga aatacggcgt ggacagctgg tcggcagggc gggatccgag caggtgtttt 540 acatgcggaa tatggccctc aactgagaga tcgcaccacg ggctgagcaa gatgagacca 600 ccaggagaag gtaaagcgtt gctttgcagc agataccgct gcagtgcaag cgcgagatgg 660 gccccagccg aatcgcctat catgatgacc ttcttggatg ggatgttgag ttcgcggacg 720 atg 723 // ID HO747793; SV 1; linear; mRNA; EST; FUN; 422 BP. XX AC HO747793; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_F06_038 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-422 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; ac2f33ca7c0be5547fbf95d7a0f48ddb. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..422 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 422 BP; 111 A; 99 C; 130 G; 82 T; 0 other; atcgagagcg agatcggcct cgtcgccgtg ctgcccatcg ggatggcgca ggtgtcgtcg 60 gtggtgctga gcgtgaagaa gggcgactgg gtggagaagg gccaggagat ctcgtgcttc 120 cagctcggcg ggtcggacat cgtgatggtg ttccagaagg acgcgaacgt cacgctcgac 180 caggagaagg gcgtccacta caagttcggt acgaaaatcg ggagcgggga gcgcgttccc 240 aagtaaacca tgcgtacgcc agtcgtggcg gtgtaacacg agtcgaattg aagagctgtt 300 tggggctcga gcgtccggat catccttctt gtactagcta tcgaccaagt atcatgtact 360 gagcaataac ccttgtccca catatgtaga tttccaacta aaaaaaaaaa aaaaaaaaaa 420 aa 422 // ID HO747794; SV 1; linear; mRNA; EST; FUN; 744 BP. XX AC HO747794; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_F08_054 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-744 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; b6cc365e8d6cd459b94bb0e789cb1d54. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..744 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 744 BP; 137 A; 225 C; 194 G; 188 T; 0 other; cccaggcttc aacatccagg gtcctcccga ctcttccagc cgttcgtcgg ccgcgtctac 60 ctctgggtca gccagtgcct ccgcgagcgg ctcggcgacc ctcactgtac cagctacgtc 120 taaaaccggt gcgtcaggaa ccgcagcggg cacctctaca ggtttcgggg tgaccgtctc 180 caactcagga tccgctgccg ccagtaacac tggtggcgct gctgcgtctg gcagccgctc 240 ttctgcgggt acggcgccgt cgaatttcaa caacggcgct gcttcccttc ctaacctcgc 300 tccttgggcc gtcgctgccc ttggggtggt cgcaggtgcc gctgctgcat tgtgatcggc 360 tagtgtaggc gtttctagga cgagttccaa gttttcgcgt agttatcgat gttcattttt 420 tacgttttca ttctgggttt tggacgccat cttacgcctt cacgcacgcg aagagtactt 480 ttacgtactg ccataatacc tgtcagtagg aaggaccggc ccacctcgtt gctcgccatg 540 tcgttgagtt gagggttggg gcctcgtgcc ccagacatct tttccatacg gaccttagcc 600 agaacattgc tgtcgggttt gaacttgtgg ttcatacgcg ttgtttcagt acgtacgcca 660 tataccaaca ttcctttagc ttacacaccg agcccacacg gcgcgaaatg cacattgtgt 720 gcgagacgag tcaaaaaaaa aaaa 744 // ID HO747795; SV 1; linear; mRNA; EST; FUN; 606 BP. XX AC HO747795; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_F09_069 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-606 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 594eb0d5fa7815ef4a0a298988e37e19. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..606 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 606 BP; 145 A; 169 C; 161 G; 131 T; 0 other; acgaatcatg cagtttatct ccaacatcga ggtcgtctac ggcgacgagc cctcccaagc 60 tccctctggc cgcgtcctcg agaccaacca ccagaacgac gacatcaact accagtttgg 120 cggaaaatac gtctggctcg tccctgtctt cacgaccgac cgcgacgccg cggtcactgg 180 tttcgacatc gccatccaag ggagcgccga cggctcgctg agcgacctcg ccaagggtgc 240 gggtggcgac taccgctacc tccttctcga gaagagcgcc acgcagacgg agagggttac 300 tgaggttgct ctgctgcgtc ttgacaatgc gatgaccgag cgtcctgcgg ggtgggctgg 360 aaacactatc gacatcaaca aaggccgcgg caagagctat ttgtacctcc tgtggaagac 420 cgcttgaagc tctcgttctt gttaaccatt gaacagctat caaacacttc ccgtagaact 480 gtggcttgcc agtagccagg atggcgatgt atctgagtgt cccgaatgtc gtagagttgt 540 tgttgctttg tcgttgtgaa tgtcaagtgc gttagcgttg tccaataaaa aaaaaaaaaa 600 aaaaaa 606 // ID HO747796; SV 1; linear; mRNA; EST; FUN; 702 BP. XX AC HO747796; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_F10_070 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-702 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; ef9250f62ef26cefed6e4f53f7fcf896. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..702 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 702 BP; 124 A; 252 C; 210 G; 116 T; 0 other; gggaaggtca aggttgcatt gcggagccca aggccaactg ggtcggtatc tactggctgt 60 ctgccaccct cacgtacact gcctccttcg ctctcgcggt caaccgctcc ctccgctccc 120 tgtcgatcaa gcccctctcg tactggaagc tcatgctccg cgacgggctc aacctgtacg 180 gcgcgatctg ggtcgtgaac atggtcaaca tgctcttctg gttcatcatc acgcccaccg 240 gccccaacga cccgatccgc accatcgtca cctctatggc cgccgtgctc accgcgtcca 300 tgtcgatgcg cattatcctc gctgtgcgtg gctcactcgc ggccggcggc tcgttcgcgg 360 tctcctcgtc gagcaacccg agccgctccg gcaacaccac gcacgtcatc tccgcgaacc 420 gcggtggggc cggccagctc gcccccgcga accccgtgct gtcgctccgc ggcaacgacg 480 gcctccacca ctcgggcgcg atgtacgccg tcccgttcgc ggacggggac aagacgggcg 540 cggactggga cggcaagagc tccgtgacgg gcgggcgcga ccagaaggtg accgagatcc 600 tccccatcga gagccagaac ggtggacagg agggcgtgca cgtcaccgtc gagacggaga 660 acgacttcac tgggtaccag aaggagaaga actgaggaag ct 702 // ID HO747797; SV 1; linear; mRNA; EST; FUN; 786 BP. XX AC HO747797; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_F12_086 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-786 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; d2bf6e93956ee396de542735613bde02. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..786 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 786 BP; 179 A; 202 C; 240 G; 165 T; 0 other; gggtcgactc tcccttcaac gtcttggctc ggcacaaagg ggccaacaat tctgctagtc 60 atggcggcac ttcgtcgttc aaatggcaaa gcacattgtg ttcgaagttg ccggctcgac 120 ctgggtttga aattgctagt tgcttctggg aggacaagaa acccgaacac aatgttttgg 180 acgttgcgta ccgacagaac cgtatggcga caacggctgc attgaggcaa cttcacgctc 240 tgaacgttca ggcgcccatc ttcggccttg tatgggcgga aggcacggtt ggagctcatg 300 tcgaatggtg gaaggaagag gacaacgatt tcactatcgt gtcggccccg tacaagaggt 360 ccacaggacg gaagcgcctc cacgactggg acctgtcaaa accggcagat atcatcgagg 420 tgtatctgct cctccggaac ctagatcgat ggaccgtcgg agggttccgg acccgagtcg 480 tggaaggtat taggagtctc gtcgaggagg tctcccaccg ggatcgccgg gttccacaat 540 ggcgacgacg cagcagtctc gggattaagg ccagcaggac ggacgaggcg gcagacgagc 600 ctgagtccat ttctagtccc atgaagccgc atggtagggc aaaggccatt gcccccaagg 660 caaagcggcg gacgactcgg cgcggtgtct gcgaattggg agcgcccacg agagcaatgt 720 ttcacgaggc ataggatatt cattgtactt ctggattcga ccatattctt ggatttgtgt 780 aatgcg 786 // ID HO747798; SV 1; linear; mRNA; EST; FUN; 726 BP. XX AC HO747798; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_G01_003 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-726 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; b8b01abba0ab6129b18a9f5814e843dc. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..726 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 726 BP; 129 A; 261 C; 150 G; 186 T; 0 other; ctccctggcc gccgttggct ttcttgtcct cctcctcttc cccacctctc cccctttccg 60 tcacagaggt tttctctccg cttcctctgt cctctgcctg acgacctttt ccgcaccttc 120 tcatcgctcg tccgccccca agacaccacc aagtcctccc cgcacgaaca agccacaatg 180 tccaagcact tctgctgctg tatccctgtc cgcgcagcgg tcttcttctt ctcccttctg 240 tcattccttg cctcaggatt gaccgcagcc attggctggt acctcgtctt cctcattaac 300 tcggacaagc ttgaggaggc cgagaagaac ctgaatgacc aggataagaa aacactagat 360 gccgttgcac acaagtacaa gtgggccttc atcgtcgctg cgggcgtctt caccctcatt 420 gctctcatgt ccttcttcgg tttcgttggt tctatcattc gcaaccgccg catggtgaag 480 gcgtactcct tcatgaccat tatcatcttc atcctcggca cggtcgcgac tggcttctcg 540 ctctacgcga ccttcagcca caagccgctc tgcgtgacga ttgataacgt ccagtcatgc 600 gcgacctcca acctcaccac aggccagaag atcggctaca ccgccttcgc cgttgtccaa 660 tggctaattg atctctacat cgtcgtgatc atccgccggt acgccgagca gctcgacgag 720 gagcgc 726 // ID HO747799; SV 1; linear; mRNA; EST; FUN; 721 BP. XX AC HO747799; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_G03_019 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-721 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 3289844fccd630ccd7340f63ca9136e6. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..721 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 721 BP; 148 A; 196 C; 222 G; 155 T; 0 other; ggcgaattgt gacgaatttg ggatgggatc tctcaatgtc tactccgccc atggacccgt 60 tgtcaacccg attggtttag aagcctgtgg acacgccgag cagaggtcag cgggagggag 120 ttcaggaggg agtgctgctg ccgtcgcagc taatttatgt gatgttgcct taggcaccga 180 tactggtggt tcagtgaggt tgcctgcttc gtactgcggt atcgtaggtt tgaagccctc 240 ctacggcatc atcagcaggt ggggcgttgt gtcctttgcg gatagcctgg actgcgtggg 300 tgtccttggg cgagacgtca gtactaccaa acgtgttcat gacatcctcg cggtgtatga 360 cagtagggat cctaccgccg caccgtccca tgtcagggaa agggcccggc agctgaacga 420 aggcagcctc gagactctga agggtggctc tctagccgga ctccgagtag gagtgccaca 480 ggaatacttc cctacggagc tacaaccata cgctctaagc gcctactacg tcatttccag 540 tgctgaggct agcagtaatc tcgcgaggta cgacgggatt gagtacggca tgcgcgtcga 600 gccgccacca ggcgccaatc tgaccaagac cgcgaacgtc tacgcataca cccgttcgca 660 agggttcggc aaggaagtcc agcggcgcat gcttctgggc acttacgcgc tatcggccga 720 c 721 // ID HO747800; SV 1; linear; mRNA; EST; FUN; 483 BP. XX AC HO747800; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_G04_020 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-483 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 1fd85b5112e3163a84ceeac82df32bad. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..483 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 483 BP; 93 A; 147 C; 138 G; 105 T; 0 other; tcgcggatta aaagtgattt gtggctaccg gtcctcactc ataaacacca aaaactatca 60 ccatgcgtca catcgctgca tacctcctcc tccagatcgg cggcaacgcc agcccctctg 120 ctgcggacat caagaaggtc ctcggtgccg tcggcattga ggccgatggt gaccgtctca 180 acactctcat ctccgagctt gagggcaagg acgtcgccgc tctgattgct gagggttcct 240 ccaagctcgc ttccgtcccg tcgggtggtg ctgtcgctgc ctctggcgct gcggctgcgg 300 ctggtggtgc ccctgccgct gctgccgagg cgaaggaaga gaagaaggag gaagagaagg 360 aggagtccga cgacgacatg ggcttcggtc tgttcgacta atccctgtag cgttagcaaa 420 acttttttcg tacccgcaca tctcaccatg ccggcggccc ggctgttatg atgatacgct 480 tgc 483 // ID HO747801; SV 1; linear; mRNA; EST; FUN; 781 BP. XX AC HO747801; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_G05_035 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-781 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 0c3df4119dbbd05c540b8c81d9951b6d. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..781 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 781 BP; 153 A; 256 C; 231 G; 141 T; 0 other; actcgactat atatcccgcc tcataatctt ccctgatacc ccgctcttcg gatacccttt 60 ccatcttatc cacctccagc ccaatcacat caaggtcctc ggaagatcgc ctgatctcac 120 tgacagtcac gtaacgatgt acgttcccgg tgtgacgaaa cccagcgccc aacgactgta 180 tcgaaggaag ggcggaggcg gaggcgggag gggaggagga ggaggaaagt caggatcatc 240 cggaagcagc ggctctagcg ggagtacggg gagtgtgggg agtcgcgggt cgacaatacc 300 tgtctccggc gcgacgaacg ggcgcacgac ggcggttgcg tatggctccg gaacgaccaa 360 ggtcgtcacc atccctcaag gccaaccctt cgcggggcgt acttctggag gtgcgacgcg 420 cgactcggtg tacgggaaca gctactacgg tagtggatac cccggcgtaa caggcctcgg 480 tgtggcgaac cgcggcttcc ccttcgtctt ctggcctctt gtgtggggcg gtgggcttgg 540 atatggcgcc gcgtacctgc acgactctcg cgagtatggc gagccaaaca actcatcccg 600 cccaggcgga cccatgacgc aagccgcgtt ctcctccaac acgtcgagct caacattcca 660 cgtcgtcgcg gacaacagca ccgtcacctc gctcatcgcg tccgtgcgca acaactgcac 720 cgtgtcctcc aactcctcct cggcgcccgc gccgttcaca gggagcgcgt cggatccgct 780 g 781 // ID HO747802; SV 1; linear; mRNA; EST; FUN; 428 BP. XX AC HO747802; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_G06_036 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-428 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 626c29c8e970df6b6df9050ef9b62403. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..428 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 428 BP; 95 A; 111 C; 126 G; 96 T; 0 other; catcgattac gttcctttgc tccgctcacc ctactctccg gaaagcgcag ccaggaggcg 60 cgctcaggct gcgcttggcc atagcccagt acgactggtg gctcgcggac ccgtgcttcc 120 tatgcctttt tgccgtgcga gcccctcgca tcgggccgac caatgaggga ctatgtaaca 180 cctgcgtggt ggaaggatga aagcgatgtg gtagcgatga ggatcgaaga tatgcctatg 240 gcgtgttgga cggggagagc gcggttctag cctcgatcaa ttcaaatgtg gcttccggtc 300 ctacttgagg ttgccagcat gacattgggg tcggtgtgca cggcacggcc tacgactacg 360 tgcatgtatg ttgtcccgcg aaggacccca gtctgtgtct tgagtttaaa aaaaaaaaaa 420 aaaaaaaa 428 // ID HO747803; SV 1; linear; mRNA; EST; FUN; 407 BP. XX AC HO747803; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_G07_051 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-407 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 1e1deeb1bcf5e618bdf7f571247bd41c. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..407 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 407 BP; 97 A; 100 C; 131 G; 79 T; 0 other; tataacgtcg gcgttgcctg cttgaacatc ggcgcgcata aggaagcgat cgagcacttc 60 ctcagtgcac ttgcgctcca ggattcatca ggaggcgaga agagcgagca gctgtggacg 120 acgctccggc gggcattcca ggccatggag cggcaggacc tggctgacat ggcgcggccg 180 gggacgaagc tggaggcctt ccgtgaagta ggctttgact tctgactgga ggactccgag 240 gagattgttg ggcgggagtc ggtccctgtg gaacggcgag agcgtggatt cagccgctat 300 gcttcgctga gtcgcagcgt ccttcgctgt gccatgtacc ctgtatacta ggctgaccac 360 tgtagcagcc attgtatagc gtgaaaaaaa aaaaaaaaaa aaaaaaa 407 // ID HO747804; SV 1; linear; mRNA; EST; FUN; 737 BP. XX AC HO747804; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_G08_052 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-737 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 46c6ed6a0bfdc9e44f91b79ac03b98f3. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..737 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 737 BP; 168 A; 226 C; 168 G; 175 T; 0 other; ccctctgcca cctcagtcag tagcatacat gtaagtaacg acctcacgac cttcacgacc 60 gcaacggcac aatactcacc atttcgttaa tcccggcttg ttttagtttt acaatgtcac 120 cagcatcgtc ttccgctcca tcgcgcaagc cgaggtgcct gacagcatct cagattgcca 180 aggacagcgg ccacttcgac cagcggtcgc aataccagaa tctagttgct ggaagttatc 240 gcgtcgactt gccctcctcc ttgaagtctg atgtggaagg ggttttcaac gagtccgcac 300 agctaccagt ggcgtgggtt acctcagctg aactacttca atacctcgat ccagacttcg 360 ttcagtttct caagtcggca aagaggacat cgccttcatt attttccgac aaggtcaaag 420 cagtggaatc gggccacctc cttggtgacc ttcacaacgt tttcctcgcg ttcaataggc 480 tacacaaatt gcgcgagtcg gattcatggt cagaggcaga ttgggccgcg caagtctata 540 acgtcctccg catctccgtt gggcaactca gcgacaacag gtctcattgt tcaatctcgc 600 tacctcaacc tctgtcagaa cacaaggcta acaagaaggc tgccgccaac ctgcacgcgc 660 gcactgtgcg gccggacgga tcgttgttca tccctggtga tgagctcgcc gaactctctg 720 atacggtcga ctctccc 737 // ID HO747805; SV 1; linear; mRNA; EST; FUN; 622 BP. XX AC HO747805; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_G09_067 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-622 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 5698a257d6c25ffce79bf1c831199f96. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..622 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 622 BP; 141 A; 208 C; 121 G; 152 T; 0 other; cacgtatatt catctctctc cccctcctac ctgtcgcatc cccctctccc atctcacacg 60 accttcatca tgcctgccaa gaagcgttgc cagctccagt ctgagccccg ttgcaaccag 120 gccgtcttgc gcatcgttgg ccagtgcccc cactgccgtc tcgagttctg tggcacacat 180 cgcatgcccg agcaccacga gtgccagaat ctggagagct gccgacaaca ggcgttcgag 240 aagaacaagg cgaagctgga gagcgaacgg actgttgcgc ctaagatggc cacagcttaa 300 cttccctcgc tcttcccttc ctcttcctcc ttaacgccac tgcgccagcc acgggggtgt 360 acagggcatg gccgtcaccc caacccgtgc atcatctcgc catgtcatct tacgtccacg 420 actccgcacc tcgtcgctat catgacacac ggaccttgct ataccacata tcatctgttc 480 cccataactc ccgatgttgc tccccaatct ggctccttca tctagtgcat aatgtttgct 540 acatcggtgt ctatttgtgt tttcatatat tctcgggtca ttatataact gtatgatttc 600 aaaaaaaaaa aaaaaaaaaa aa 622 // ID HO747806; SV 1; linear; mRNA; EST; FUN; 733 BP. XX AC HO747806; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_G11_083 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-733 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 97768cfae3d3704e38ad346d277e5ad2. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..733 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 733 BP; 144 A; 197 C; 206 G; 186 T; 0 other; ggttctctgc agtcgacact gatcgttcgg gagcgattag tgttacggag ctccaggccg 60 ctttggtgaa tgggaactgg accaggttcg acctggatac ggtgaagatg ttgatgtcga 120 ttttcgacac cgaccgcagt gggaccatcg gtttcaatga gttcgcggga ctatggaagt 180 acatccaaga ctggcaaaac gtcttcaggc acttcgaccg agatcggtct gggtccatag 240 agggagccga gctctccgag gctctacgta gctttgggta caacctgtcg cccacgctcc 300 tggcgctgct cgagcagaaa tatgcttccg gtccttctga ccggtacggc cctccgcctg 360 gcatcacctt tgaccgattc gtccgggcgt gtgtcgtcgt gaagacgctt acggaagcgt 420 tccaacgggt tgacacggac cgcgacggat gggtgcaaat gaattatgag cagtttatga 480 agatcgtcct gagtgcgcct taatcgcgcc tcgccctggg agcgtccacg actgtcagca 540 tgtcgtccgc ctgccttgtg tataatcttg ccatgattgt catgaccgta ccgtatgtcc 600 ggagcataat tcgactgcta gactcggaac gatagtccct cctctctggg ccctgtatgc 660 atctctctgt acgagttgtc ctgtctccca aagacttctg tgacgatatt gtcgtggaat 720 acttattcga gtc 733 // ID HO747807; SV 1; linear; mRNA; EST; FUN; 722 BP. XX AC HO747807; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_G12_084 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-722 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; fadb531cf63e8a4a14802d1d41b67601. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..722 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 722 BP; 148 A; 193 C; 185 G; 196 T; 0 other; ggtcctaacc aacaacccta cgtcactggg tctctttttc ttccacccga tcttacagag 60 cctatcgatt gcattgttca cctacggcat attgacactg cagcccacgt cccaagccaa 120 gaccaaggcc gccggactga ccaggcacca attggcaatg atgggccttg gagtcccggc 180 gatccttttg ggtacccttg cgatcgttcg ccggaaggca ttacatggga gttctcattt 240 caccacatgg catggaacgc tggggatcat ttccgtgtcg tggctggtga tccaggtgat 300 gctgggcggc ggaagcgtct ggttcggagg acgcttgttc ggcggcaatc cgaaggccaa 360 acaggtttgg aagtaccata ggctgtccgg gtaccttctg ttcccgcttt tccttctcgt 420 agtgcacctc ggaggtgctt ggtcggactg gtcgactggc gctagtgcct acttcgtccg 480 ccttttagcg tttacgataa gccctatggt attgcttggg gccatccttg ttagacttcg 540 gacttcgaag atgcaattct ggtaggactc cttaggtccc acacagatgt gtggcatgtt 600 ccggcagtct cgttctgtat gtgtacaata ttctcgtgat actccttcat acgttatgcg 660 atcgtacttc tcttcagaat gtcaagccaa tcggttggtt ccactcaaaa aaaaaaaaaa 720 aa 722 // ID HO747808; SV 1; linear; mRNA; EST; FUN; 756 BP. XX AC HO747808; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_H03_017 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-756 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 192bd524ddb20d343b3881d338af8073. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..756 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 756 BP; 194 A; 193 C; 215 G; 154 T; 0 other; aggatctacg tgctcgagaa gcaaacccgc aatgcttcgg acatgaactc taccacccgc 60 ctcgtgaagg aggcttccga attatgctac aaggctggga atctcgattt gctgaatagc 120 aatattaata cattgagcaa gaagcatgga cagctgaagg cggtcatcca agctttggtg 180 gagcaggcga tcggttggct tgaggagact cgcaagtccg cgggcacaga gaagtggttg 240 cagctcatcg agactctgag aaacgtgacg gagggcaaga tcttccttga aactccccga 300 gcacggatta ccctccttct tgcacaatac catgaatccc ttacaaccgc cgcttctacc 360 acgcccgctt ctcggaaaga atccctccag ctcgcgtcgg atctccttag cgacctccag 420 gttgagacct actcgtctat ggaccgccga gagaagacgg agtttatcca ggagcagatg 480 cgcctgttga tcgctcttgc tcgtctcaag gacgctgaga ttggcggtga aggcaaggac 540 tcaatcggcg gtggcgaggc tgagtgggta aaggtgcggg tcggtggccg gaaggtcaac 600 gaagagttcc tcaagagcaa ggataatgag gacctcaagc tcaagtacta cgacatgatg 660 atccagtatg cgctgcacca gggtgcatac cttgatgccg cgaagtatta ccacaaagtg 720 tgggagacac catccatcaa ggaggacgtg aatggt 756 // ID HO747809; SV 1; linear; mRNA; EST; FUN; 781 BP. XX AC HO747809; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_H04_018 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-781 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 29ffa27ae25300eca16f9860b07257b3. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..781 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 781 BP; 169 A; 227 C; 198 G; 187 T; 0 other; gaaagctctg aacactcttc tttctggtgc cttattcctg ttgctgggtg cttctggagc 60 gtccactggc ctcgacgtca gcactacctc tgggacattc agaggcgtcg ccaatacaac 120 tgggatagag cactggcttg gtatcccatt tgcagaggct cccgtcggtc ccttgcgctt 180 caaggcaccg ctccctttca agaaacgatc gccagtggtt caaagcgcgt ccaccttcgg 240 caatgcatgc cctcaggcgc cttcgaacag cttgggggcg cctcaatcgg aagactgctt 300 gttcttgaac gtctttcgtc ccagcaaagt cagtgcagca agtagaatcc cagtcctcgt 360 ctggatacat ggcggcgcgt acatgagtgg tgccgcctcc gatccggagt ttgatcccac 420 gtttatcatt caacggagcg tggcaattaa caagccgatc atattcgtgt ctatcaacta 480 tcgcatcaac acatttggct ttctggcgag taaacatgtg gccgcgtcgg acttgaacaa 540 tggattgcag gatcagcgtg cggctctcga atttattcaa gaaaatatcg cagcgttcgg 600 cggcgatccg acgaaggtca cgatatgggg acaatctgca ggggcaggca gcgccgaagc 660 acaggtactg ttcccttctt ccaaagatct cttcagagcg gcgatctttg actccgccac 720 tggtccattc aaaactgcac ctcccgccag tacctacgac cgaccgggtt tcccttacga 780 a 781 // ID HO747810; SV 1; linear; mRNA; EST; FUN; 756 BP. XX AC HO747810; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_H06_034 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-756 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 072f5b44d6e383b15c7553341696222c. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..756 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 756 BP; 162 A; 231 C; 205 G; 158 T; 0 other; ctccctccct ctcccctaat ccttactccc ctgacctggg ctgtctttta tcttccgata 60 tggcggcaac acagtataat caagatcagc tccgggagct catccagggc ccaagcgaca 120 tagagtggac gtatgagatg aggcgacaat gccaggagat cctgccccat ctgttccttg 180 gccctctcca agcatccaag tctctggata ccctcaagtc gctcgggatt acgcacattg 240 tttgcatacg cgacgcgaag gaggcgttct ctgtacggcc acggttcccg gagcacttcc 300 agtatatggt gttggatgtc caggatagcg aggagcagaa cctcattcgc ctgttccccc 360 aggcgaagca gttcatcgac gacgcgatcg caaagggtgg ttgtgtgctt gtacattgca 420 atggcggcat cagcctgtcc ccctcgtttg tcgtgatgta cgtcatgcag ttttacaacc 480 tgtcctggga ggacgccctg cacctggtcc agaaccggcg gtactgcatc tcgcccaacg 540 gcggcttcat gacacagatc aaggagtacg agtcgatcta caaagccaag gccgccatcg 600 cgtcgcaccc gcagcgcctc ttctccgtgc ggaggaagcg ctcggatgag gacgacgagg 660 accaggtcca aagggagggc gaacgtacga aacgcgccct ggtggacgag gatgatgacg 720 acgcgatgca gacatagtca acccttcttc cctccc 756 // ID HO747811; SV 1; linear; mRNA; EST; FUN; 805 BP. XX AC HO747811; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_H07_049 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-805 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 48facc6b5ca5f80b987fdf3007f7e9ef. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..805 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 805 BP; 146 A; 254 C; 206 G; 199 T; 0 other; ggatcgaacg cgttattgag tgtggcgtcg tgccgcgctt tgtcgagttc ctccgtggcg 60 acaatgctat gcttcagttt gaggctgcat gggccttgac caacatcgcc tccggcaccg 120 ccgagcacac ccaagtggtt atcaatgccc aggccgttcc ggaattcatc aaacttctgt 180 cttctcccgt ccccgatgtc cgtgagcaag ccgtgtgggc gttgggtaac atcgctggtg 240 acagtccgac atgtcgcgac tacgtcctcc agcagggtgc tctccggcct ctcttgaacc 300 ttcttagcga gaacaacaag ctcagcatgc tccggaacgc cacttggacg ctgagcaact 360 tctgccgagg caagtcgcct cagcccgatt gggatctgat ttcgcctgca ctcccagtgt 420 tgacgaagtt gatctactcc ctcgacgatg agatcctcat cgatgcgtgc tgggcgattt 480 cttacctttc ggatggttcg aacgacaaga ttcaggctgt tatcgagtct ggcgtttgca 540 ggcgtctcgt cgaccttctc atgcaccctt caacgtctgt tcagactcct gcccttcgct 600 ccgtcgggaa catcgtgacc ggggatgacc tacagactca ggtcgtgatc acatccggcg 660 ctcttcccgc ccttctttcc ctcctttcct cgcccaagga cggcatccgc aaggaggcct 720 gttggacgat ctccaacatc actgcggggt cgccacagca gattcaggct gttattgagg 780 cgaacattgt tcctcccctc atcaa 805 // ID HO747812; SV 1; linear; mRNA; EST; FUN; 484 BP. XX AC HO747812; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_H08_050 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-484 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 8a54f8888c1d6ca2e005c2baa682832b. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..484 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 484 BP; 96 A; 169 C; 127 G; 92 T; 0 other; gttaaagcca ccgcgtgaat tatacagata tgctacgttt cagggagagc acatcggata 60 accacccaca ccttggccac ccacgaaaca caggacatag ccccactatg tcgccccgta 120 cttcggtttg aagtacgctg gcggtagctt cgcaaggacg tccaccagcg gccgctcaac 180 cacgacaagc tccccaggcg cgacgaagtc gacgaacagt agcggccggt aatgcgtcac 240 cagcttgaag ttgcgctgct gcacctcctg ctggccctct tctacggtca cctcgacccg 300 cccgttcgcg gtggcattag cctctggggc cttccgcttg ccccgcctcc gcttctcgaa 360 gcccccgtat ccgatgcgct cggtgagcat gaccttgcat atccacgttg cgccccagaa 420 cagcgcggtc tttggaggta gtggggagcc ctccgcattc gaactaccat cgacgcgcga 480 gccg 484 // ID HO747813; SV 1; linear; mRNA; EST; FUN; 559 BP. XX AC HO747813; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_H09_065 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-559 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 3fcb46f8ac287f1e003de61a2a765858. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..559 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 559 BP; 173 A; 151 C; 155 G; 80 T; 0 other; ggcgcccaca cccctcgaac gcatcatgac agctccgaag ccgaagccga ctagcggcgc 60 gatgatgtcc aaggtcggga accctcaagt ttacgaggac ggtgaccaga ggaacgctcc 120 ggctggagaa cacacccagt cgccctcgca cgacaagccg gaacagttgg cgggaggtca 180 gatgaacgcg cacagcatct tggatcctaa ggataacagg agcccccgta ttcacgagaa 240 gcaggagcgg aaacaggaac gggaagccga ggaagtggag cgccacaaga cggtgacgaa 300 ccctctcgag ccagcgttga agcacggaca caagccttct cgcggagcgc aggtcgatgc 360 ccagttgcag cacgaggacg aggaagcgtt gatggcgaag ggtccctact acgggatgtc 420 ccacaatgct aaacacaagc acgagcatgc cgagtgatcg ttgagtggca aacctcttgt 480 atcctagctc aaccgatgct tctgtagcaa tatatacgtt tcatcctaaa aaaaaaaaaa 540 aaaaaaaaaa aaaaaaaaa 559 // ID HO747814; SV 1; linear; mRNA; EST; FUN; 769 BP. XX AC HO747814; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_H10_066 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-769 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 004f7dea2aa814533700e298a105e3c7. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..769 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 769 BP; 149 A; 228 C; 194 G; 198 T; 0 other; cccgtctttc ctgtcccttc ttcctcgtat cataaccatg ggtctctccg tctcccggct 60 cctttccggt ctcttcggca agaaggagat gcgtatactg atggtcggtc tggacgcagc 120 tggtaaaacc accatcctgt acaaattgaa gttgggcgaa attgttacca ccatccccac 180 cattggattc aacgtcgaga ctgtcgagta caagaacatt tcgttcactg tgtgggatgt 240 cggaggacag gacaagattc gtcccctctg gaggcattac ttccagaaca cccagggtat 300 catcttcgtc gtcgactcca acgatcggga acgtatctcg gaggctcgtg aggagcttca 360 acggatgctc aacgaggatg agctccgtga tgccctcctc cttgtcttcg ccaacaagca 420 ggatctcccc aacgcgatga acgcggccga gatcactgac aagcttggcc tccacggtct 480 ccggcagcgg acgtggttca tccaggcggc ctgtgcgacg agtggtgatg gtctgtacga 540 gggtcttgag tggctgagcg ccaacatcaa gcgacgggtg tgagcggtgt cctagccgtc 600 tgggttcctc ttcaacattg ccgaacgttc atgtcttatg atccttacga tatcgaagag 660 ggtttcactg gttacctccg cctcctcttg ctcacctgtc cgccgcagcc ttttccccgt 720 ccgctacctt gacttttctt ctgtcttctg tgccatataa cgtaactcg 769 // ID HO747815; SV 1; linear; mRNA; EST; FUN; 674 BP. XX AC HO747815; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_H11_081 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-674 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; c9480619add510f76b95f20cce8dc717. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..674 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 674 BP; 144 A; 174 C; 218 G; 138 T; 0 other; agcgatcggc agtcacctca ggagggtaga ggcaacccag gacgaccact tgccgatgtg 60 catccaccgg tgctaaaccg tagagactga tcgctatcgc gggcgatgca gagactacga 120 gtgagcatcg cggtgatgcg actgccaggg gttagaggca tcgatgaggc cgggcaccgg 180 acttgatacg cataggccac aggcgtgccg tgcctaagtc catggagaga ctaggacgga 240 cgcaggcagc gattaggtgc agcacgacgc catctggcag cgcgcccagc ttgggaggtt 300 gtgactcgcg agaaccaaga gcccgtgtac cttcatcgat gtattcgcag actgtgagat 360 acacggtctg cggtatgtgt tcgtgtcgtt gttgacgcga gcagccgttg gtctgtaccg 420 aggccacggg cgcgcgatgc ctgtggaacg agtacggccg aggaaagcaa atcaaatacc 480 ccaggacatc cccgcacggc tctcttcgtc ggactctctt gtagttgctt gtctgatcca 540 gttgggaggg cccgactgag gaagtccgga tggctggact gctgataagt acaacacata 600 tggcgtctgg cgtagtgcag tggtgcgctt gtatgacgcg gttttgtcaa tccaactgtt 660 gttgtgcatc aact 674 // ID HO747816; SV 1; linear; mRNA; EST; FUN; 673 BP. XX AC HO747816; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050052.T3_H12_082 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-673 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 955ace0a60537f8bc2258a940505e1d3. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..673 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 673 BP; 145 A; 220 C; 163 G; 145 T; 0 other; ggtcgaattt ctatctacac ccccacatct tacacctcgt tgaacaccca tggcccgtac 60 caagcaaact gcccgcaagt ccactggtgg taaggccccc cgtaagcagc tcgccaccaa 120 ggctgccagg aagacggcga cggcggccgc gaccggcggt gtcaagaagc cgcatcgctt 180 ccggcccgga acggtcgccc tccgtgaaat ccggcgctac cagaagtcca ccgagctcct 240 cattcggaag ctccccttcc agcgtctcgt tcgtgagatc gcccaggact tcaagaccga 300 tctccgcttc cagtcctccg ccgtcatggc cctgcaggag gccgctgagg cctacctcgt 360 ctctctgttc gaggacacca acttggctgc tatccacgct aagcgtgtga ccatccagcc 420 caaggatctc gctctcgctc gtcgcctccg tggcgagagg tcttaagcgc ctagcgcccc 480 ccggttctcg atacaggcct ggcccttcgc tatagtacac tgtattccgc ttcgtcgtat 540 tcgctcgtat tgtctggtct tttcgaagac cttgtattgt aatgcctcaa tatgcggtag 600 gagtatgtat acggtccggt attggtagat gaaatcgata tctatgcccc agaaaaaaaa 660 aaaaaaaaaa aaa 673 // ID HO747817; SV 1; linear; mRNA; EST; FUN; 696 BP. XX AC HO747817; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050053.T3_A01_015 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-696 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; ffcf8eedbabb8f9b63ec03e241db02fa. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..696 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Ltd.) The phagemid pBK-CMV FT harboring the inserted cDNA from Ganoderma lucidum BCRC FT 37180 was mass excised from the Lambda phage, with the FT helper phage co-infected bacterial host XLI-Blue MRF'. The FT released circularized, packed phagemid particles after FT infection and multiplication in XLOLR bacterial host were FT plated on a Q-tray, picked by robots by white-blue colony FT selection, first stored in 384-well microtiter plates, and FT further multiplied in 96-well plates for DNA extraction and FT subsequent sequencing. Colony PCR was performed by T3, T7 FT primers in 384-well format to prepare templates for FT sequencing, and sequencing was performed in 96-well format FT by the T3 primer only. Sequences were obtained from trace FT files by base calling with phred 0.020425.c while setting FT the value of the phred parameter '-trim_cutoff' as 0.01. FT EMBOSS vectorstrip and BLAST of EST sequences against NCBI FT UniVec database were applied to remove vector and adaptor FT sequences. BLAST of EST sequences against all reference FT sequences of microbial, viral and mitochondrial genomes FT from NCBI ftp site was applied to remove suspicious FT contamination of sequences (sequences with blast hit whose FT E-value is less than 1e-20 will be regarded as FT contamination). Only those with more than ten contigious FT A/T will be regarded as poly-A/T tracks." FT /db_xref="taxon:5315" XX SQ Sequence 696 BP; 150 A; 179 C; 227 G; 140 T; 0 other; gacagtgagg gtgactgcca gacagcgatc ggcagtcacc tcaggagggt agaggcaacc 60 caggacgacc acttgccgat gtgcatccac cggtgctaaa ccgtagagac tgatcgctat 120 cgcgggcgat gcagagacta cgagtgagca tcgcggtgat gcgactgcca ggggttagag 180 gcatcgatga ggccgggcac cggacttgat acgcataggc cacaggcgtg ccgtgcctaa 240 gtccatggag agactaggac ggacgcaggc agcgattagg tgcagcacga cgccatctgg 300 cagcgcgccc agcttgggag gttgtgactc gcgagaacca agagcccgtg taccttcatc 360 gatgtattcg cagactgtga gatacacggt ctgcggtatg tgttcgtgtc gttgttgacg 420 cgagcagccg ttggtctgta ccgaggccac gggcgcgcga tgcctgtgga acgagtacgg 480 ccgaggaaag caaatcaaat accccaggac atccccgcac ggctctcttc gtcggactct 540 cttgtagttg cttgtctgat ccagttggga gggcccgact gaggaagtcc ggatggctgg 600 actgctgata agtacaacac atatggcgtc tggcgtagtg cagtggtgcg cttgtatgac 660 gcggttttgt caatccaact gttgttgtgc atcaac 696 // ID HO747818; SV 1; linear; mRNA; EST; FUN; 784 BP. XX AC HO747818; XX DT 02-JAN-2012 (Rel. 111, Created) DT 16-MAY-2013 (Rel. 116, Last updated, Version 2) XX DE HMA050053.T3_A02_016 GLEST05 Ganoderma lucidum cDNA 3', mRNA sequence. XX KW EST. XX OS Ganoderma lucidum OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; OC Polyporales; Polyporaceae; Ganoderma. XX RN [1] RP 1-784 RX DOI; 10.1371/journal.pone.0061127. RX PUBMED; 23658685. RA Huang Y.H., Wu H.Y., Wu K.M., Liu T.T., Liou R.F., Tsai S.F., Shiao M.S., RA Ho L.T., Tzean S.S., Yang U.C.; RT "Generation and Analysis of the Expressed Sequence Tags from the Mycelium RT of Ganoderma lucidum"; RL PLoS One 8(5):e61127-e61127(2013). XX DR MD5; 8fddc5433b761eae8ab206ff215c7816. DR StrainInfo; 523525; 0. XX CC Contact: Ueng-Cheng Yang CC Advanced Bioinformatics Core (ABC) facility, National Research CC Program for Genomic Medicine (NRPGM), Taiwan CC National Yang-Ming University CC No.155, Sec.2, Linong Street, Beitou District, Taipei City 11221, CC Taiwan (R.O.C.) CC Tel: +886-2-2826-7359 CC Fax: +886-2-2825-0480 CC Email: abc@ym.edu.tw CC Seq primer: T3 Reverse CC POLYA=No. XX FH Key Location/Qualifiers FH FT source 1..784 FT /organism="Ganoderma lucidum" FT /lab_host="E. coli XLOLR" FT /strain="BCRC 37180" FT /mol_type="mRNA" FT /dev_stage="18 days" FT /clone_lib="LIBEST_026664 GLEST05" FT /cell_line="A2B2" FT /cell_type="Haploid" FT /tissue_type="Mycelium" FT /note="Vector: pBK-CMV; Site_1: EcoR1; Site_2: Xho1; The FT cDNA library of Ganoderma lucidum BCRC 37180 (GLEST05, FT monokaryon) was constructed by ZAP Express cDNA Synthesis FT Kit and ZAP Express cDNA Gigapack III Gold Cloning Kit FT (Revision B.01)(Stratagene Co. Lt