ID FN619612; SV 1; linear; mRNA; EST; INV; 671 BP. XX AC FN619612; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-671 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 23b8068c22f1c5bf5c71a0ec4e90625c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..671 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 671 BP; 216 A; 138 C; 161 G; 155 T; 1 other; ttagcgagga agcaaagata ctataatcag gttttgttgc gaagccgata gaagattagg 60 tgcgcaaaga ggtatagaag aacttaaact agcccctttc ttccgtggcg tcgattggga 120 acatatcaga gaaaggccag cagcgattcc ggtcgaagta cgttctatcg acgatacttc 180 caatttcgac gaatttcccg acgtgaaatt ggagatacca tccgcgccaa tgcctcaaga 240 tggggaggta atatacaagg actgggtatt cattaattac acgttcaaac gattcgaagg 300 tttgacgcaa cggggcacgc caaccaagaa atagcaacac tctatttctt gctcggtttc 360 gaccagtcag tagcctgacg caactggaat tccggccttg gtacagaacc cgtaccctcc 420 gatgtcatca acggcatctc gaacggatgg ttgattcgtt ttatcttctg aatacggtcc 480 agtcaatcat cggcagtcag acgcttttac atgtacatac gtgaagtgaa atagtcacga 540 cgtacgtaca taggtacacg ttatcgtatg aaaatgcgaa agaagaatta gaaaagaaag 600 aagaggtaaa aggacaagaa agaacatgac aaagacgata aaaaacgtcg tggtgtttta 660 ggacnaagga c 671 // ID FN619613; SV 1; linear; mRNA; EST; INV; 652 BP. XX AC FN619613; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-652 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; c56d3f19a36705c723e601cf2603adf4. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..652 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 652 BP; 252 A; 90 C; 118 G; 191 T; 1 other; tggatcgcaa ttatcggagg aaggcgcttt acgctcacaa tttacagtgg atgatttgga 60 tattgaaata ttgccactta tatatgaaat aattcggagt gttgagaaag atccacatga 120 cactacacag aaaactaaag agtcacaaga tacaagtcat aaaatattag agcttcaaaa 180 gaaattagat tccgcaaggg cacagattag aagattgcca ggaattgaat atagtaaaga 240 agaacagttg cagaaacttg aaactctacg taaacaactt aggcttaaaa gagagctttt 300 attgaaatat cgtaacatgt gtacatttga agtcccaaaa gtataaataa ttttgcttgt 360 ttaagaaaaa gcaatttata tcagcatggg atttttacgc atgttattta catatttgat 420 gcgtaatgaa aaattaatcg agaaattagc aaatacaaaa ccaattaggc agtctgcacg 480 atttgtagca tatttgttac ttcacagtag aacacatggt attcatttat cttttgatcg 540 tgaaaaattt gttaggcaat taaggctgtt tacaaataca tttagacaaa agttgcaaga 600 agctaaagat gaaatgaaga anaatccacc aaagtagtcc ttgtatatat tt 652 // ID FN619614; SV 1; linear; mRNA; EST; INV; 704 BP. XX AC FN619614; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-704 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e56e039ad79e99b68e61aad5371fcf5e. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..704 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 704 BP; 214 A; 128 C; 137 G; 225 T; 0 other; ctttaatttt ttaagatgcg taaaatctat ggtctagtta ttatcgatat tatattcttt 60 cattcaaaat atgcaacgtg ttgctaaacg aaatggggaa atcttcctta tcggtcgcca 120 gtctgttaat aaaatctgag tcgatgtgtt ctaagtttta agttctaaga gttaagaggc 180 ggctgtgctt tagatacaga gtcgggaaga aaagattcat ctgattactt ggatggctca 240 attaataaag tactctctct ggtttagaca acgtagacga agaagaccgt gctcaattga 300 attatccttt ctcttcctac gataattcac gttcgcgaca aatcttattt ggaggaaaaa 360 ttttgatgtc tcgcattatg acctatctcg atggatcgct atggagatac gacacctaaa 420 gagcttaaat tttcatctac gaataaccag gcaatcacac tcgagattat ttgtctgttt 480 cagcaaaatc cacttaatag tggatagatt tttcatttga aaaactccta cgatatataa 540 gtattcattc agctcgtctt tagtttcacg ttacactact gggtaattgg gatgcaacct 600 tggtgattga cgctcgacga taaattcgac cgaaacgaaa atacaagagc ggagctagaa 660 catttcgggg gaattatgac tcacgtgtgc tcccactgaa actg 704 // ID FN619615; SV 1; linear; mRNA; EST; INV; 716 BP. XX AC FN619615; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-716 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a064f8735d768ff71ec4bcb2d759f362. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..716 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 716 BP; 234 A; 115 C; 113 G; 254 T; 0 other; aatagcttgc atttaatttt cttttcctcg cttattatgc acccgcgtca ttaagtcctt 60 ttttttcacc tacgatcgtt acgggaaaaa ccgtgaacat tgtcggtcgt tactgcgatt 120 tttgctagta gtttcccact cgaaaatttc cagcctgttt ctttcgacca atgcaattaa 180 aaaatcatta ccattttgaa taactttgca ggatatctac agaggagagt aatttagaaa 240 tttaaagaaa cttaaacaga gattaaaatt tgctgtgtga aagaaaatag aataacattg 300 aactaataat tatcatggag gaggtgatct ccgtcttctt ataattagca tttcattctt 360 caaattgcta atgattttta tggtaatgcg tgatttttta aatattttac acgacgcgct 420 ggtacacttt taagagcaaa atcgattatt tcgtcataat cggtggacgt gtaaactttc 480 tacgctgccg tttttatatt attttctcgg atgttgtata agcggcatac cagcaggcaa 540 gaataaacgt attaagtttg tagagaaacc tgtattttct tcgtaaatat aaaatgctgt 600 ataaaaataa aagaataatg ttcgtaagct ttataaaaca cagcaggata tttgtgaacc 660 attttctcat gaaaattata aatcacatta aatcctacgt tttatttaaa atcata 716 // ID FN619616; SV 1; linear; mRNA; EST; INV; 684 BP. XX AC FN619616; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-684 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e137d2351deebbd6284df24b91f73b8c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 684 BP; 223 A; 116 C; 135 G; 210 T; 0 other; taatttttgt agaacaaaga aaaaggtgga gagtattact aaaactattc gtcgatgtgg 60 gtggccagca gtatgtatac atggcgacag gtcgcaatta gaaagagatt ttgttcttac 120 agaatttaga agaaataagg attcaattct tgtagcaaca gatgttgctg ctcgtggatt 180 ggatgtcgac gatgtaaagt acgtgatcaa cttcgattat ccaacttcat ctgaagacta 240 cattcataga attggtagaa cgggccgatc aaataattca ggcacgagtt atgccttctt 300 tacaccacaa aatagccgac aggctaaagg tttaattaat gtacttaagg aagctagaca 360 agttatcaat ccaaaactta tggaacttgc agacagaact ggaaatgatc ttgcacgaaa 420 tcgttggggc tatggaaatt atcgcagaag agaaactact ttccctaaaa cacataaacg 480 attcgataat ctaagttact ttgacgcgta gttggtttaa ttcaacttcc gatcacaaac 540 tttctgaaca agtaacctgc tggagatctg aaatttatta caatttgttc ccctgtgatt 600 agtaaaagtt ctccgttaag ggtcctattg ctttacgtta gttccgtgct cggttaaatt 660 ttttaatttc tatttataaa tcta 684 // ID FN619617; SV 1; linear; mRNA; EST; INV; 718 BP. XX AC FN619617; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-718 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 6cbf5b61da773bdb7c1458438a182f68. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..718 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 718 BP; 250 A; 94 C; 90 G; 282 T; 2 other; tagtacataa cctcattatt tcgattggaa tatctcgtga ataataacaa atatgattaa 60 tccacttcaa aggttatttt ctttcttaaa gtattggtac tttagataca tgcttgttac 120 ggagttatac atggttgaag aatgggaaag aaactttatc aatatatttc tgatcacgtt 180 gttcttttcc tttttctggt ttaattgtaa aataatgctg cctattatac aatacgcatt 240 acactcaaaa gaaatattgt cttaattcac tgtgtttatt gtaacacaat tatactatgt 300 tgtgttttga ctaaagcaaa aagaaattat aacttatagt taatattgca tgttaaacca 360 atggtaaatt tctacattca aagttatgtg ttaatctgtt acattatttt aataaattaa 420 attaatagaa ttaacgtttt actttacaaa ttaagttaat ttaatttatc atataaagaa 480 atacgtcttt atatatccaa gaccatgtgt tatatgtata gtaaaatgct aagcattcat 540 tctttctata cgtaatacaa ttttggcatt tttcaaagtg taatgtgatt gctgataatt 600 actctgtnca ggtcaaagat aattacatac atttagaaca tattcctggt attttctgta 660 ttaataatag caaacatcac acagatagtt tttcantaca ctgacatttc tatatgag 718 // ID FN619618; SV 1; linear; mRNA; EST; INV; 398 BP. XX AC FN619618; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-398 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; f5a4d66e9fff0106cfd49b44d50cbde9. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..398 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 398 BP; 116 A; 76 C; 67 G; 139 T; 0 other; cacatacgtg gatgtgtgta tggatagcaa acaccagcgt attgttaagt tattgctgac 60 cgtatttgta cattccctag taactctatc tacagttcga tgcattccac aaacaactac 120 gattggtttt aaccattaac taacccttct ttaacgggtc gactgtcgga tcatgatgcc 180 atattagtgt tgtatatctc aatatgataa tctgaatatt ttaatatttt taattaattt 240 cgttgtcgat cattagtatc atccacgtga tcactttcta atgtcggtca atgcacaggt 300 tctagcaacc tccacaacgt ggaggtcaat gcgttaacga ttatttcgat atgaaaatta 360 cggcaattta atctatttat tcgacgttta aacaagtg 398 // ID FN619619; SV 1; linear; mRNA; EST; INV; 514 BP. XX AC FN619619; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-514 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 77882eea7c5efcd17b64f9c49ef503f4. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..514 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 514 BP; 203 A; 57 C; 92 G; 162 T; 0 other; cagtttttgg aagtagaggt gttattaaca aagaaggaaa tgataaaaaa gcgtattgaa 60 ggtcgtcaat tggcacaaaa tgttggagaa caagtaatta tacttggcac tattggaaaa 120 aaaggttcaa acggtagaaa tatagaatta aaaacaaccg atggtgtaca agttaatgta 180 accttaccag taccaattga taacgatgct gaaggttata tagaaataca tggcacatta 240 caatccgcat ctacaatgaa ttgcagtaat tatatagtat tcccacttag tatgacagaa 300 aattttgatg tcgatcggta taaacaactc atggttatct tgaatgtatt aggatcagga 360 aaatggaaaa tgtctgaaga tgaaacaggg ttttaaattt ataattgtat aacctataaa 420 aatgcttaaa aactttttat aagaatacga ttattttaat atgtatgtcg aagtgtaaat 480 tattctttta taataaattt aaatagtatc ttgt 514 // ID FN619620; SV 1; linear; mRNA; EST; INV; 714 BP. XX AC FN619620; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-714 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 1460ccbbb126b44dc8a1f83e4bc047f5. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..714 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 714 BP; 202 A; 158 C; 165 G; 188 T; 1 other; aaaatctatc gcggtacaca gagttttcac gagacgtatc gttcccacat atttccagtc 60 aggatgagtc ccgtattggc cgttgagtta ttgttcttcg ccatcttcgg tcagacgacc 120 cacgaacaat tcaaggtcga aaaaacgcag cccgagtgga ccaaagttct cttcaagctc 180 acctttggta tttatatgct ggtttcggtg gttgtgctga ttaatctgct aattgccatg 240 atgagtgaca cttaccaacg gatacaagct cagtccgaca tcgaatggaa atacggattg 300 agtaagcttg ttagaaatat gcacaggaca agcacagcgc catcaccact gaatctcctt 360 accacctgga tggcatattt gtacaaactc tgcaaaaagc gggctgcaaa aaagcaacga 420 ccttctcttg tccgcttgat gggattgcaa agaaccgatg cgctttcagt gcggtccaga 480 atgggcgcca agtggttgtc caaagtgaaa aaagcacagg tggctcacaa ggatagcgtt 540 gctttatcgg ttgttcacct gagtcctctt ggtagccaat tatcattcag taacgttgtc 600 agaatcgata atgttgttga ttgggaagcg gtccgacanc agtatcgtga tctttatggt 660 gaaaatatcg agaaacatgt cgaagaatcg aaagaatcta ccgatcatga atct 714 // ID FN619621; SV 1; linear; mRNA; EST; INV; 644 BP. XX AC FN619621; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-644 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; cd417714fe5d546a4cc563252e552e8f. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..644 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 644 BP; 159 A; 184 C; 160 G; 140 T; 1 other; gaggaatcgt aacgtaaaac tatatcgcgg ttacaccagt tcctcgcgcg ttccaaattc 60 cagaaatatt ctagaaactt cgctaacgaa gcagctcgcg actttaaaat ataaggtaaa 120 tcatgcggaa gatcagaagc tgcggtcgag tatcgtggaa cggatcgact tggatcgatc 180 acgcgaacgg cgcacatatt ttttctctat ttttacaaag gactacgctc cactttcatt 240 aaccgtcgta cgtgccaagt gtcaagcgtc cctgcaattg ggacacccgg tagacgaagg 300 taagcgtctc cgtgccaacg ttccaataac cacccaagag cacaaggaca gtctacccca 360 gttttcctat tgcgaccgcc tacggcacgt ccttcacccc ctctcctctc tcgctcgctc 420 actctctcct ctgtgctcgg tcgctcttgg tcgactctct tccgggccga tcggcgtgga 480 aaataccgcg ggaaatcgat ctcgaccgga cggagaggaa attcgtgagg cgtgagacca 540 cgccgcgcgg ataaaagggt ggacccggcg tggaaaggcc gacgccagag agggtgatca 600 tccccttcgt cnaagacgga ccaagaaacc gcgttggttt cagg 644 // ID FN619622; SV 1; linear; mRNA; EST; INV; 671 BP. XX AC FN619622; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-671 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 9fe6fccbb5c33bd84197f5164e5adc1e. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..671 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 671 BP; 221 A; 120 C; 143 G; 187 T; 0 other; ggcagagtgg tcgatactcg atattacggt accacatggt aacggaggta aaatggcgtt 60 aactgttcct tgtgtcgctg tgagaggttc cattggaatc agcttaggac aaggcccacc 120 atcatatgaa ccagtgaagt cgtttcctaa agatgacagt aaaacatgta aagcaatgat 180 ttttagccca gagggaagtt attttgcatg ggtcaatgga gtaactacca agattttaca 240 ttgttctacg tggaaaatta ttacagaaat taaaaggccc aaaatttctg cgattcagtt 300 ctccactcga agcacatatt ttatgacgtg ggaaccattc attgcgacaa catcaaatcc 360 tcaaggaaca ccaaatcttc acatatggag atcagagact ggagaactgg tgaagagttt 420 catacagaaa aaacaatcag actgggaacc tcaatggaca agtgatgaaa aaatctgtgg 480 gctattagtt ggtgcagatg tcattctata tgaggatgtc aattttaaca aaattatgta 540 cagaataaat gtagccaaag ttgctagatt tagtatatca ccaagtaata ctccgtacta 600 tattctctgt tatatgccgg gaaaatcgga tcagccttca tttggtaggc tatttcaata 660 tccgaagttc g 671 // ID FN619623; SV 1; linear; mRNA; EST; INV; 659 BP. XX AC FN619623; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L16 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-659 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 28c526167dda8fd0813bd60db8df9b01. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..659 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L16" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 659 BP; 204 A; 114 C; 148 G; 192 T; 1 other; agacgagtta ctgtacttag ttccattatg gggttcgatg tgaaccgctt tcagggcgaa 60 gttgatgagg aacttgtttg tccgatatgc tccggtgttt tggaggatcc tgtccaggtg 120 agcaatatgt tgcaggcacc tgtatgtgaa catgcctttt gtcgtacatg cattaatgaa 180 tggataaata gacagcctac ctgtcctttg gatcgtacac caattacatc tgctcagctt 240 agagcagttc caagaattct tcggaatctg ttggcccgcc tttgtattag ttgtgataac 300 attatgtacg gatgtcaagt aatagttaag cttgatagtt tagtgtcaca cttggagcaa 360 tgtgaatata atcccaagag accgatgctt tgtgaacaag gatgtagtct tattattcct 420 aaaaatgagc taaaagatca taattgtgtg agagaactca gaaacattat acattcgcaa 480 caacaaaagc ttgctgatat gaaacgtgag ttaggtgaac aacaacttca aataaatgaa 540 cacaaaaggg aaatacatct tttgaaagac tttatgagag cattgagagt ttcaaatcca 600 gcaatgcgtg ccatcgctga tcaaatgnaa agagacgaag ttgtaagatg gtctgctac 659 // ID FN619624; SV 1; linear; mRNA; EST; INV; 671 BP. XX AC FN619624; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-671 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0acaae1eb027f115d0f90751e94e0670. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..671 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 671 BP; 178 A; 132 C; 148 G; 212 T; 1 other; ggatcctgtt ctccggtccg atttccggtt tccttcgaga cccagcgact cgtctccgcg 60 aatcgatgta tggttaagtc gcgacgaata tccagtcatt aggtccgttc gaagtgtcgc 120 gacgagtcaa gaggaaattt cttgactggg acggacactt ccgtgcagcg cctccttcga 180 cacgcgacca tcaatcttaa acccgcgaat caacggactt ccggttgtct gccttccccg 240 tatttcctta acaatagttg ttttttttct gttcatctat tttcagttgg aaagagaggt 300 gagaagaatg gattgtttaa aaagagactg ttaagcaaaa gagaagtgta aaatgtttat 360 cgaaacgttc attactgggt cacgatattg ttacgagata cggtacattt ataacaactg 420 atcaattccg ctgtttgaaa attcctttgt tttagtatta cagcacgtga actatattgg 480 tggactgtgg atttttatgc atttgttcat gattattcca ctagataatt catttttatt 540 ctcatttgct cgtataactt aaaaatgcgc gttgacctgg cgcatagcca aacatgttaa 600 agatcgaagg tcagagaaat tttgcgcaaa gtgaagtcct tggagttgnc gatttgacga 660 ttggaaatgg a 671 // ID FN619625; SV 1; linear; mRNA; EST; INV; 605 BP. XX AC FN619625; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-605 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8b140926d8a3efbb7475cefe7d2b5114. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..605 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 605 BP; 211 A; 98 C; 137 G; 159 T; 0 other; aagattcaaa cagcgattgt cgtcgttatt tcgcagctga aaaaggaaac aaacgaaacg 60 aaggaaaaaa gaaactgtat aaatacgtgc atattgtgaa cattttttag actcaggatt 120 tagcgttcct cgcatgttgt aataatctca aaagcgctac gttttttatc gccgtccatg 180 aaagaggatc gtgattgaac agatgaaaat agaaactctc ctattgtttc gtatgcagcg 240 tgtacgacaa ttaaaaacca gcacgatgtc gtatatcaaa ttgaatttta acatggagcg 300 aacatcgtgc aacgttgaag aagggaaatc tgtaaatgca aaactcgtga cgttagcgtg 360 caaactttta ggtcatttaa aaagagaaga gccaagagga ttaattatga aatagtcaat 420 acgttgaatc attgtgatac tgaggtggaa aaatcgcaag acgtagcgaa tgctgtggtt 480 gtacgagcca tcgaagtcag ttggaacgac gtttttcttt cgtctgtcaa tggaaaagac 540 gggaatttcg aattttcatc gttgacaaga ttgccagatg catcgaaaag agaaacacag 600 gcgga 605 // ID FN619626; SV 1; linear; mRNA; EST; INV; 461 BP. XX AC FN619626; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-461 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8feeb05e36811833772ceb592205aa18. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..461 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 461 BP; 139 A; 72 C; 110 G; 140 T; 0 other; gattagatca ctgtgaaaat acaatgggtg cagtacgtgt aatcaatgac gatggccaat 60 tttatgggga aatggctagt gcaggtgcaa aattggtcgt agttgacttt acggcaacat 120 ggtgtggccc ttgccagaga attgcaccaa tatttgagca attatcaatt aaatacccac 180 atgcagtatt tcttaaggtc gatgtagaca agtgtgcaga gactgctgca atgcaaggag 240 ttaatgcaat gcccacattt atattttatc gtaaccaaac gaaattgggt ttatgtcacg 300 gagctgatcc ggctggatta gaatcaaaga tacaacaatt ttatggcagt ggagattcag 360 aggattctga gagtccagtg tctggacatg tacgagatag tgataatgtt tattaccata 420 tttttggaat atttcttgat gcagttttgt acttctggaa t 461 // ID FN619627; SV 1; linear; mRNA; EST; INV; 699 BP. XX AC FN619627; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-699 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e30b28c7884eb2588c9c658fa92452cf. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..699 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 699 BP; 249 A; 102 C; 141 G; 207 T; 0 other; tcacactgac acgattgcga cataaaagag aatgcgtggc attttgtcga gcgaaaactg 60 cttaatatat aaaataatat atcgataaaa tacctatgta tcaagatttt cttgttaacc 120 ctatccatga gaagtgctaa atcgtgttag gggatctttc tgtacgtaca acagggcaac 180 atttaagtca ttaagaaaac attagataag gaagattgaa ttcacgttac atgtatatgg 240 catgatgatt tgatatgata tgaagatact tccatttaaa gtcttaataa tatatttaat 300 gatttcataa tttcgggtct aatgtcgaca acaaatatgg cagcgacaga tgatacaaaa 360 cttgatttta aagttacaat ggaatatgcc gaccctcaga atatcgaagg tgagctaaca 420 gtaggaggca aacaaaaatc aaatctcgga gaacctgaat ttaatacttt ggatgaacca 480 atcagggata caattctcag ggatgtcaga gcagtgggca ggaaatttta tcatgtttta 540 tatcccaagg agaagaaatg cctattaaag gaatgggatc tttggggacc actagtatta 600 tgtacattca tggcaatgat attacaaggt tcttctgata cagcaaataa ttttaatgat 660 gggggaccag atttgcagaa gttttgtaat cgtatggat 699 // ID FN619628; SV 1; linear; mRNA; EST; INV; 689 BP. XX AC FN619628; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-689 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; bb3ea04b4a859b4deaef0418ab091327. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..689 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 689 BP; 224 A; 102 C; 115 G; 248 T; 0 other; ctagtcagca accatacttg cagctacagt aattaagttt ttacatttac tacctcgtcc 60 cattgaacag actatacata tcaatttttc aaatgcttat acatcatttg tataatagat 120 agaatatatg tatgtattgc ttaagtctgt tcgaattgaa tgtttctttc gcgagggggc 180 tagaatctaa taagaaacaa taagacacat tatttacacc attagtcacc ttgttaatag 240 tgatgtatta aatagaaaca cttgatatgg aataaaaaat tgcattacgt aattaaaagt 300 gtgggaaaat ttcaaattca ggtttgaata tctctatgtg aggctaacca aacacggata 360 taaaattgtt attgaaaatg aaattaatgg caattttgga tcagtttgaa ttctctgtat 420 tttctgattg aatttttgtg gacaaacatt tatattatat aaatttatgt atcaaatttt 480 atgcattttt atattctttg tggatgcaag tgcggagtta tgtacgtgta tccgtgcggt 540 caatctccta ttttcacgcc gactgctcag agagtaattc gtttctgaac gcttcgcagc 600 ggaagggtta aaataaactg atttttatac acagcatttt atttactgtg tgcctgattc 660 atagacgcag atcaagaaat tcatttcta 689 // ID FN619629; SV 1; linear; mRNA; EST; INV; 683 BP. XX AC FN619629; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-683 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 5f4a9d639b15f90d586b2d8b057fb43a. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..683 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 683 BP; 233 A; 102 C; 97 G; 250 T; 1 other; agacacatag taatcgaatt tataaaaggc gcgaaaaaat tctctctctt ggttcgacgt 60 ggaaaaagca tttttcatcg ttgtaagtga ataacttcga ttttctcgta gaaaatatcg 120 tcaacgggaa agcaattatt ctgctaatgc aagaacaata atgttctttc gtgttttagt 180 ttcattctaa ttgtgttatt aaatttctgc ttgtgtttta actaagttcc tcccacgttt 240 tctccctatt cacttttcat gcagaaaata acgaggagaa gggaagaaga acttgtgaat 300 tttttcctcg aattttaacg aacaaacgtt atggcaaaca gagccgagcg gttagatttc 360 tccagctccg caatttctat tcgataaatt cgacattctt tattatttat caatgactga 420 tatttacgaa tatttatgaa tttatggaaa attcaaatat ggaacgatac aaattactat 480 aaaatatcta aatacaattt tttaaagatt tataataatt gttataattt tggttctaat 540 atttattgag taaaatctat ttacgactat ataactttaa aaaattacaa aatcatctac 600 aattcactta ggtatcgatt actaaatgca ttttccaatt tatctgntta tcatgtacac 660 gtgttggtat atcgaataaa gta 683 // ID FN619630; SV 1; linear; mRNA; EST; INV; 625 BP. XX AC FN619630; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L23 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-625 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 3a11e258bbaf62150bf406b88e3eb714. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..625 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L23" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 625 BP; 167 A; 99 C; 147 G; 211 T; 1 other; attgcatccg aaagggttga ccgtgttaat ttgttaattt aatcgatatt tcatcgacgc 60 tggaacacga cgacgattta tgcttgcgcg aataacgcga ctaatattac acgatgtgca 120 gggggtgtaa aaagggtaga agagcgagag agaagggtaa aacggtgacg agctggttgg 180 atatattgac agtgaattgg ctcgatatat tcacgctaat tattcgtagg ctatagtgct 240 tcgcgtttag ctctattcat ctataatgaa gtgcaaaggg taagctcgat aataactgta 300 tcgtaggggc gtgactgtag ggtggttcgg ggttgcgagc ctatggggtt tcacgcgtat 360 tagaccttca ttcggatcat ctaatattta gtgaatcttg ttacatcgtg aaaataacga 420 gttacgtgtg tactttttct tttttctttt tttaatctca cgatcgtatg gaagatcgag 480 agacgttcgt aattgtacat ccgattttct tcaaatttta aagaattaac ccacgtatta 540 tgttttacaa cgtggcgtaa gatttcttcg atatcttcat ttttcgatat tttttangaa 600 cgtttaaagt cgatgtctgt ccggt 625 // ID FN619631; SV 1; linear; mRNA; EST; INV; 688 BP. XX AC FN619631; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050L24 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-688 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 922465c66549074409384ab26118ed41. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..688 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050L24" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 688 BP; 277 A; 75 C; 167 G; 169 T; 0 other; taagccatgt ggtaggagtg aaataggtta acattcgttt gaaaaagcat tttgtaaata 60 tgtcattttt tattcgaaat aaacaaggta gcggaggaat taaaagaaag tttaatggtt 120 atactaaaac aaaggggaag acaaagaaga taaataagtc tatacctgat gataatgaaa 180 gtattaccag tacagacgaa gaacttgcag aagaaaatca gagaaacgaa gattatgaaa 240 gcgaggaaga ggaagaaact gctcatgaga agcgtgtaag actcgcaaag aaataccttc 300 agcacatcga agaggaagaa agagataggg cagaatttga agaaggagca gtagcaagaa 360 gattgcaaga ggaatattta gaagaaaagg gaaggttacg gaagactgta gcttcaaatt 420 atatcaatca tggtgaacca attgttctaa aatgtaaaga acataaaagt tcaattacct 480 gtttatgtct ctctagcaat ggaaagtttt tatattctgg ttcaaaggac ggtagtttag 540 ttaaatggtg cttaaaagaa agagcaaaat tgaaagccgt taaagggaaa aggaaatcag 600 aggctggaat gaaatgtgta caatgtttgg cgattagtac cgatggtaaa tttttggtag 660 caggagatag cgggtgtaaa catattct 688 // ID FN619632; SV 1; linear; mRNA; EST; INV; 759 BP. XX AC FN619632; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-759 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 3c56b3b27961fdc4fa7a8e51d745e4ce. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..759 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 759 BP; 261 A; 148 C; 125 G; 225 T; 0 other; gtgtcttgtg tatctcccgt gttccgttta tagttataaa gtgcattctc gtatatgatg 60 gcgtccgcga aaagaaagca agacgagaaa aatttaaaaa tattacgcga gctggtctca 120 caacctggta ataaaaaatg tttcgattgc catcagcgag gcccaactta cgtcaacatg 180 acaatcggtt cattcgtctg tacctcttgt tctggtatgc tgaggggctt gacaccacca 240 catagagtaa aatctatttc tatggcaaca tttactcaag aggaaataga ttttataaag 300 gaacgtggta atgaatattg tagaagaata tggttgggct tgatgaacca aaattcatca 360 cagactttag atacaaaaga tgaacaaaaa atgaaagatc tagtgagtgc aaaatatgag 420 cttaaaagat attatttgga tccgtccatg gcaaatcaga actcaaatca aaaatcacag 480 tcatctaatc aaactaacat tccaagggtt ccacattctg gaacatctac tacaacatta 540 tcttctacaa catctacatc agcttcgaaa acaaacaatg ttgaatcagt taattttaat 600 aacttttcaa cagattttgt agctgatttt tcagcaagtc ctgatccatt ttatactact 660 acaacacctg gcaataaact cagtcaacct attgtacctc agtcattctt tgccattttt 720 gacacatccc tgttttgata gccaaagata caatattga 759 // ID FN619633; SV 1; linear; mRNA; EST; INV; 726 BP. XX AC FN619633; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-726 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 93e36c792b088ef508e329a623d6fe33. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..726 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 726 BP; 207 A; 179 C; 129 G; 210 T; 1 other; aggctttctg tttggagctc cgtggtcgct cgtcgttaac caaccgcacg tgctacagtc 60 cgacgcgaca gtcagactta cgtccctctc ttcctgtccc tcctactctc tctttctctc 120 tttctctttg actctctatc tttctacaac cgagctaaga aattaggctt gctttgatac 180 atagagaaaa ggatgaaaaa agagaaaggg aaacgcatag agctaaaaca ctacaccacg 240 accgccataa aaaatgcgaa agatgaaata tgattttcat gcacacgtcc aacgatgacg 300 ttttaccggt tggttttata acttcgataa aaaccgtgat tttaacgtcg catcgttgcc 360 ccgttttatt cgaaccatta aaactccgca agtaaaaaca tcttatttat gtacgccgtt 420 gctcgtgctc ctcacgttaa aagggggaga aacagggcag atcgatgcat ctcatggcag 480 aataacattt atcggcgaaa gaagaaaaca accatctttt ttcttctctt tgcctatttt 540 tctctccctt tccctttccc attacgtaaa aggcgttcgt aaaattcact ctcgagcaaa 600 gttaacgcct ccctttgact ttgtttaaat caattccagg gcattaagtt ctctctgaat 660 cagccgggca tgaaacgcgc caagccaata atgagttnca gcgtaaactt tggatataaa 720 tacata 726 // ID FN619634; SV 1; linear; mRNA; EST; INV; 699 BP. XX AC FN619634; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M03 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-699 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 980d83e791e2ea81256f8dc70664d2f9. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..699 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M03" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 699 BP; 197 A; 149 C; 159 G; 194 T; 0 other; tcgttgtcag tcgtaaacca tggaaatgtg taagccactt aaaatgcgct gtacgtcgtt 60 ctccgttatt ctactgtgca ttctcaagtg tgggtttgtg cttgcgatat gcactgtgag 120 caagcacgaa ggaggatcag cccatcgttt gcaatgctct aacgttactc tgcaaacgat 180 tttggaacaa acgacgaacg tatccgctct attggtcttt gactcaaatt tccgatacat 240 caaagaacga gcttttatca aatacgctaa acatttgctg ttcttgaaca tctacaattg 300 tcacttatac gacattcacg ctgacgcgtt cgacagccta gctagtttga ggaaactcag 360 tctacctagc aataatatca cagaagtgaa agaggcgtgg tttaaggatc tggtatactt 420 ggaacaatta gacctgtcgt tcaatcgaat cgagaaaatc gcgccagcaa tctttagcag 480 aataccatta ctcaaaagat tcgatatcag agagaatcgc ataacttgtt tggatccttc 540 agcatttccg ggtggtatcg acaaagttta tttcctcgga aaccccctgg ctctcacgtg 600 tcgaggagag ttaactctgt ggatgcggga ccacggggtg aaactacaga ctgagcagag 660 taagaaggaa gagtggttgg ataagcttct ttggctctg 699 // ID FN619635; SV 1; linear; mRNA; EST; INV; 727 BP. XX AC FN619635; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-727 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 09abdb3c557ff0eb9fcb4fede12d3077. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..727 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 727 BP; 170 A; 168 C; 153 G; 235 T; 1 other; tacgaattcg aataggcgaa agtggtattt ttgttcttgt ttttgcgtat ttattgtttc 60 atacgtttta atttttcttc aacgaaacgc attcgatcgt tttacgcata tatatatgtc 120 tttgagttta acgcgatgct cgcagagttt ttctcttgtc cgtgtacatg taatcggtaa 180 atgcgcgcgc gcgcccctga aatcatagga atacgttact acgcgcgctt acctgtcatt 240 gtgccagcca gcttaatcgt ttcagtttct ctcgaaatta ctccgttctt ttttttcccc 300 ccctccccct tcattgaaac gcaatatgtt cgattcgttt cgaaaaccag tcgtccgtcg 360 tgtcgctttt gtctcttcgg tcctcgttgg ttcgtggctt atatgctttg caaaatcgca 420 acgagaagat tcgttcgatg atcagcgact aaatgcaatt ataaatctca tagaattttc 480 tgtctgatcg cgtaatttag tgacgcgaaa caaattggcg cggtaccttt cacgatcgct 540 cgtatcaaat gacgcgatcg atgccccagg agggcacgaa acgtattatc cgacgataag 600 caacgagatg tcacgcgtaa agcgttaacc gagaatccga tncatttttg cccgtatttt 660 cggtgtaacg agccgataac gttcgatcat ggtggagaac attgcccgtc gtgctcgaac 720 gtatcga 727 // ID FN619636; SV 1; linear; mRNA; EST; INV; 623 BP. XX AC FN619636; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-623 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 83ab9fb7daf0e1bb3f8278f7545abd08. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..623 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 623 BP; 188 A; 101 C; 120 G; 214 T; 0 other; tgtgatcccg gctcgtgcct agcatttttc attcaaagat cgttattgca tttttgatct 60 caaagtacaa tatattggtt agaaattatt aatttttagg tcatcgtgaa aaaatataga 120 tataactgca gtaaataata ttatacaata aaatgaacga atttaagttg cctaaagctc 180 ctaatggtat tggagttacc gcttcatgcc ttgcagcggt cggcgtgacg ggttatggct 240 tttggaaatc tatgtataca gtggaagctg gtcatagagc aattatattc tctcgtttgg 300 ggggaatcca acaagatata ttaactgaag gtcttcattt tcgtattcca tggtttcatt 360 ggccaattat ttacgacatt agaagtaggc ctaggaaatt atcttcccct acaggatcca 420 aagatttaca aatggtcaat atttctcttc gtgtgctgtc tcgtcctgat gcaactatgt 480 taccatcaat gtatcgtcat ttaggtcttg attatgatga aaaggttttg ccaagtattt 540 gtaatgaagt attaaaatca gttgtagcaa agtttaatgc atctcagcta attacgcaga 600 gacaacaggt ttcaaattta gta 623 // ID FN619637; SV 1; linear; mRNA; EST; INV; 702 BP. XX AC FN619637; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-702 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; de5e3b4d466aaabb31addb31b241eee3. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..702 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 702 BP; 265 A; 92 C; 75 G; 268 T; 2 other; gctgcaagct agctcatatt agtaactgcc caatcacctg taactgtgat ttatagttct 60 ttttatattt atttttacat attttacgtt accagataag aaatagcatt atcgaattga 120 ctcatacaaa atttattgtg actataagga ccataaacaa tatgattatt tcataagtaa 180 tggaaagata ttaacatgct atacaaccaa ttctttttaa atccatgtaa aatatttgta 240 tgctagttag actgaactat aaaatgtaat ttgtcattaa agttaatatt ctttatcata 300 aacatgaaag taaataatac ttttgcaaac atttatacat attcaggtac caaaaaatta 360 tataatcatg caatgtttta aagagtttat aacatagaaa aatgatattg tagtggctac 420 ttctcaattg ttttccataa atgtcatcaa ttttataaac tttacaagat gaatgtaaaa 480 tttgcgttta atatttgtaa attaaatata tctgtaaatc attttagttc acttcagata 540 aaaaattaaa aatattttaa atcacattat catcgtgaag ataatgtatt tttaatcctt 600 ttacaattac agncaatgct ttatactgta taatgtacaa cttcaaagtc catatttcca 660 actactgnaa atcgtcattg attttagaac gatatttttt ta 702 // ID FN619638; SV 1; linear; mRNA; EST; INV; 633 BP. XX AC FN619638; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-633 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a09ea453395c6df19efd3dd8882f1e28. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..633 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 633 BP; 214 A; 82 C; 116 G; 220 T; 1 other; gttacactat tcatcatggc gagccgcana gcttcgtggt aagctctggg tgtcactctt 60 tttgtgcaat tatcgaatgg ttctgtatat tcgaagttgt tacgaaaagt aagcgattca 120 attaaaaaat aaatgcaaat attttatact aaaatatgat tagagacacc attgcaacca 180 taacatttac acatatgtaa atgtttcaat gtaaaaggag aggttatgtt ttacagtatt 240 ttcgtgattt tgtaagattc agaatttata taaatattcg ctccgaaaat taatatatct 300 atagttatcg ataggaagat ttagattata tttgagttgt atacaatgga cggagtagga 360 ttatttaata cttttcttca atcacaccaa cctcaattag aattttctgg ggtgccaaga 420 atatattggc acattttatt taaaaaactt cagtaccaga tatttgattc aggtctgata 480 tttcaattaa tgagaattga ttacgatagt gatgagaagg gtgacaagga tcctatttgg 540 aagctctatg tttgtcgtaa agaaggcatt gttgttaagg atccgaacaa tatttatttg 600 atagatcatg catggacgta tgatattaac aaa 633 // ID FN619639; SV 1; linear; mRNA; EST; INV; 647 BP. XX AC FN619639; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-647 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e00d40b380602b94540ee4fe6d57f9ad. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..647 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 647 BP; 254 A; 100 C; 120 G; 172 T; 1 other; tgattcatgc gacctttgtt tatgttggca acatggtcac tgcaatgcaa tagaaaagga 60 aaaggatgta cctgagaaat atgtttgttt tatttgtcgg aatccatatc gccaacgatt 120 atccaaaaaa tattttcacg atcaagattg gataaaagag ggaaagctac caacattatc 180 tagcagaaca aaaaatcggc atcgagttaa ccaaggaaca gctatgttaa aacgttctta 240 tgatctagtt gctgctcttt tacacattca gcaagtttta catagtttaa gagtaaaaat 300 caatgtagct caaaagaaag atcgtccaaa gctatatctc tgggccaaaa attgggaaaa 360 agttgaggta ccaaaaccaa atgttacacc agtaccaatc atggagatta tgaaagtagg 420 aaaagagtgt acagatattc caagtgaagg ttttcatcga actgaagtaa aaacagaaac 480 aaagttcagt ttaagagatg atcatgatga anagtcgata gcctcagatt cagaactaat 540 gaagatatta gaagaagata atataactcc agatgaatct aaaattaacg gcaaaaagga 600 agatattata catcgaacta aaagtcatat tcttcttgat gcactta 647 // ID FN619640; SV 1; linear; mRNA; EST; INV; 507 BP. XX AC FN619640; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-507 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 52624ec0d13d798d4ab7edf33574d1ea. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..507 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 507 BP; 164 A; 67 C; 73 G; 202 T; 1 other; tgacaaacgt anatggatgt atgctttctg aatttccgaa gctaatgata attagtattc 60 gctaattgtt cgtatttagt tacgctctgt atatacatgt atgtaggtac attttttaat 120 taaatgacat atgtacatat atataatcat atacgttatg ctaaagcggt attacgttgt 180 acgcggctga cacgcgtaga cacgaatatt ccttcctcat cgatgtattt taagtatttt 240 aacatttgac gatccatgta tctatatgat agaaaatcat cgttattttt attacaatta 300 tcatcattta aatatctgct tattagtatt tttgttatcg ttattaaaat actagtatcg 360 tagatatagc aattgacaaa aattggaatt tttatgttta aaatcaatca tacgatcgtt 420 tgtttcgttc gattcaaatg tatctatatg tgattaacat tttattgtaa atctaaatat 480 atcgcaaata aagttttcgg aatacgt 507 // ID FN619641; SV 1; linear; mRNA; EST; INV; 678 BP. XX AC FN619641; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-678 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 63dfbb5bde96169ae59ae288304254c6. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..678 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 678 BP; 218 A; 114 C; 142 G; 203 T; 1 other; tggtgcaact gtattaggag ttcatttaga aggacctttc attagtccaa gtaaaaaggg 60 agctcatcca gagaatcata taaaacaatt tgaaaaaggt ttcaagtcat taagtgatat 120 gtatggaagt ttagaaaatg tttgtcttgt aacattggca cctgaacttc ctaatgctca 180 atctgtgata acagaattgt gtaaaagaaa tattacagtt tcccttggac attctgtagc 240 aaatttgaaa gaaggagaag aggctgtaaa gaatggagca tcctttatta cccatctttt 300 taatgcaatg ttaccttttc atcatagaga tccaggattg gttggtctct taacatctga 360 tcaagttcat cctggaaaaa ttatccatta tggcataatt gcagatggag ttcatactca 420 tccagcagca ttgcgtatag ctcatagaac ccatcctgaa ggtcttgttt tagttactga 480 tgcactgtca gcgctaggat tagaagaagg tatacatcaa ttaggacagc ttanaatcga 540 aatgcgctcg ggacgtgcat atattgctga gacagatata ctgtgtggaa gtacagcaga 600 aatgtcgaaa tgcgttcgtc atttcaaaga agtaacaggt tgttcaatag tagaatcttt 660 agaggccgcg actctcca 678 // ID FN619642; SV 1; linear; mRNA; EST; INV; 644 BP. XX AC FN619642; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-644 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 9b150f9d4709b3e42f6cfcf04d1dd844. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..644 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 644 BP; 204 A; 127 C; 112 G; 200 T; 1 other; gcgagtgtaa tggctgacac gtattggatg taaatatatg cacgtcctta ctttttatac 60 agtgttaaat tctactttat cgcgaaggtt attcgcatta tactcatttt cctaaagcga 120 ctaagcatag atactgcgat ggctgacgag ggatttgatc tgtgcgaata catgcggagc 180 gaatttgcta tgcaacgtct gcttttgatc ttgcgacaaa gtcaaaatta ttgcacagat 240 aatgaatgtt ttagcatttc gcgattgcca ggaccacgcg atacttcgtc gtcctcaaac 300 tttttcatga ccactttgtt aattattgga ttcgccgttc ttatgtatgt tcttagacca 360 aactctcttc gtcaattaac caacaacaca gctaaagatc gagataacga acgcgattca 420 aacggcgacc ctcccgtacc tccaccgaca gtgcaataag aaatgcaata tgacaaacac 480 tggattttca tagtagaatc tgccaaactt atgataacag aagaactggg gagagaataa 540 ttaattcatt cattcgattt ataaaatata actagtatat atttaatcta accaaataag 600 aanctatact tcctttcaca ggtttgagaa tcattatatt tata 644 // ID FN619643; SV 1; linear; mRNA; EST; INV; 697 BP. XX AC FN619643; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-697 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 1ace2ae2bb624cf0002555024b116cb2. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..697 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 697 BP; 262 A; 91 C; 134 G; 209 T; 1 other; ataaccttaa atattttacc gataagggaa gcaatttatc ttttaatata tggatatatg 60 tgtatgttgt tatcgttcaa atattgcaga aagatggaaa aacgtagatt tcttgtaaat 120 ctaacaaaag ataaaagaat tactcgcgtc tttgagagat gtttctcaga tgccactgca 180 actgataata atgagaaaac aacacatttt ggattcaaaa ctgtcaagga aagtgataaa 240 gcgaaagaag tatatacagt ttttgagaag gtagcaaatt cgtacgatca aatgaatgat 300 gctatgagtt ttggaattca tcgcatttgg aaagatatat tcattcaaag gcttggacca 360 actcacggca ccaaattgtt agattcggct ggtggcacag gagatattac attcagatat 420 ttaaattatt taaagaatac ccctaattac caagatgtaa aaagttctgt aacagtttgt 480 gatataaata agaatatgtt agacgttggt aaagtaagag caaaaaaatt aggatggaca 540 gatcaaaata attttaatat tgattggaag caatgtgatg cagaanagct tcctttcgag 600 gatgattcgt ataatgctta cacaatagcc tttggaataa gaaatgtaac gcatattgaa 660 aaggtacttt ctgaagcata cagagtactt actccgg 697 // ID FN619644; SV 1; linear; mRNA; EST; INV; 654 BP. XX AC FN619644; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-654 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 2273f8ece468abedc6de2f17ecda2197. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..654 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 654 BP; 206 A; 137 C; 109 G; 201 T; 1 other; cgatggatta ctcggctggc cattttaaca ctccgtggta tcaatatcgc taccattgac 60 aaatcgaacg tgtgattctt tttataattg aaactacgtt ttttatctca tgtgccgata 120 aatacataaa cacgacgata ttgcgagaaa aaagaaagat tcctacgatt ctattaccac 180 accattcgcc ggcaataaat cagaaaatgg aactccgcct gatgatctct gtcgaagtcc 240 tcgctggatg ttatcaaatg aagaatcgaa ttcccgtgta tcttccatcg aaatctttat 300 taaccttgct accagtcgac tataattctt tattcataaa tacgactcta ataatcgaca 360 tactttgatt tgattaatat ctgattgttc aaaatccaaa catgcgggat gatctaaacg 420 aaacattgtt ttctcaaaaa ccaaagacga agggacaaag agactattct tttggcgtac 480 attaaattgt tctatcacgg agaaacagag attgggtcat cctttttaaa taaacggcgg 540 tattaataaa aatcaaactt ttattaatac agcttgtctc ctgcgcactt ggattcgtta 600 tncaggcttt acagccgatc gtaactcgcg caattcacgt cgttcgatct gccg 654 // ID FN619645; SV 1; linear; mRNA; EST; INV; 647 BP. XX AC FN619645; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M14 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-647 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 1d30a05a121cd14f5dc04b649497d705. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..647 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M14" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 647 BP; 197 A; 89 C; 120 G; 240 T; 1 other; atacttctgg tatacgaagg agcgtcctat cgcctgatgg ttttaggtta aactctgtga 60 tatttattaa acaataaaat atgcgtgcaa ctattgctat attgtgcgtg ttcctcggtt 120 gttgtagcaa cgtcgttttc ctggaacttc ttgttgagga tgatccaggc agtggaaatc 180 ttattacatt tgcacagttc ctttttatat caatagaagg atttttattt acttcaaaat 240 gtgggactgt aaagcccaat ataggaataa aggattactt tatattagta acaatgtttt 300 ttattgcaaa tgtttgtaat aattacgcat ttgattttaa tatacccata ccattacata 360 tgatattcag agctggttct ctaatagcta atatgatcat gggtatcatt attttaaaga 420 agagatatgt ttttagtaaa tatttgtcag tatttatgat aacccttgga attgctattt 480 gtactattgt tagcggcaaa gaaattaaat ctttgcgagc caagaatgta gaacaagtac 540 ctacaacacc atgggatgat ttgttttggt ggatattagg tatatcatta ttgacaattg 600 cactatttgt atctgcaaga atgggtatct atncagaagt attgcat 647 // ID FN619646; SV 1; linear; mRNA; EST; INV; 692 BP. XX AC FN619646; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-692 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 3504952432b8d89361b5e29e738575c3. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..692 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 692 BP; 225 A; 88 C; 127 G; 252 T; 0 other; actggtgatt actaatttcg cataacgttt tgaatgtgaa acagtatgct atgataaagt 60 tatttgttaa tcaggattaa ttatataaga taatgtttaa tctttacgta agattaacat 120 ttatatacga aaatagttga ttgttcattc gtttgcgtcg tatctttgaa aaggtatagg 180 agttacatat gcaatgatta tgtaagtttc tatatgaagt gctgaaatat ggcaatgttt 240 ttatgtaaca attagcgtat tgaaaacttg gatctgtagt atgtataatt atatcgcgaa 300 cattacagag acacgcgaac ttaacgattt cgtcttgtcc gtcgtgtatt cgccaattat 360 ttcataatat ctgtttgcaa ctgatttaat gcgtatttta agaacaggat tattatcgaa 420 taaaatgttg cgtacctagt gtgaaatggt cgcaagaatt atagtggcaa ctagcaaaag 480 catggaaagc gatttaaaat atacgacgac atggcctttg gtgcacagta taaacggtta 540 ctttttttat tgcagtatat atttataaat tcttatgtac atttagcttg tacatagaac 600 gactctgatg aatgaatgat ttcatatatt ttataatcgg atgcgaaaaa gagtatagtt 660 ttcgcttccg cgatatagca ttacgtcatt tt 692 // ID FN619647; SV 1; linear; mRNA; EST; INV; 760 BP. XX AC FN619647; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M16 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-760 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; c7c977b330237c263b4b6b1e1acbe5ae. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..760 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M16" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 760 BP; 274 A; 123 C; 106 G; 257 T; 0 other; cacgattctt tatttttaag aattattaga gagaatttaa ttgtttgtga gaaatggata 60 atcaaacttg aataaagcaa ttcataactt ttgaacagat ttattacacc ttgatatcat 120 tcaaatgctt taaatagtaa aacattttta tcggctattg ccacgtaaaa attgtttggt 180 atgaagtaag aaaggttaga atatacatat aaaacaataa tataactcac attaaatatc 240 acaaacgagg aatgaggaaa ataatttatt ttacttccag tataaacatt tattgaagac 300 tacgaaaaaa tagaatgtct gtttaagaga aatgaagaac ttccctcccc ttacgatgtt 360 ctgcgcttac tgtggattac ggaatagagt taccaaacgc gcatgcacgt agcttaccgc 420 gaattactca taatggttca aattttttaa ccttttttca ttattaaatg actcttacat 480 aatttttctt cttgtatttt attgtaataa tatattttat tgcacaatat tgaaacacat 540 gtttagaata aattctaaac actactaccg tctttactgt gcatgcgcgc ggctgtttga 600 aatacaaacg ttctgaagct tctatactct cattccagtt ctgatacagt gggctccagt 660 taaacgacac tcaatgtacc gcaaaacatt gtccatttca ttgtctaaaa aaagatcagt 720 gaaaaatgta tatccaataa aagttcaaaa atcgtatacc 760 // ID FN619648; SV 1; linear; mRNA; EST; INV; 719 BP. XX AC FN619648; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-719 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 10fea27b0ae1452769381f97ad090ef0. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..719 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 719 BP; 212 A; 120 C; 153 G; 234 T; 0 other; ggggggcgca aagcgatcgt ttattgtgtc gggagaaact gtcggtacag gtttacgtca 60 gttttccttc acttttttcc caacgtgatt agctaagaat aatatcgatc gaacgatagt 120 gaagacaagt gaaatgtttt agtggtaaaa ctgattaacc gtacggttgg attttggata 180 tcgtgcaatg aagtttcgag tggactactt tccatttact agaatacaat taatgttgta 240 ttttaaataa atcttgttaa tgagattctc tggtttactt tagaattgag tgttttcgat 300 agaaaatatc gttgtcgtac cgctttagtg ccatcgtcgg aaaagtgcat aagccagaag 360 taacattatg cgatcgataa ttcgccgacg attttgaaat acgcttcaat gattcgatca 420 ggtacttgtg actctgtgca acgtttcctt agttgtcagt gagatgtttc aaaagtgatt 480 tttcagtgtc ttccaagacc tatgcacgac gaaataatag caagtacaaa ctatatactt 540 gttggtatgt gtacaagcga tgctaggaac agtgtcgtcg ttttggacaa atacattttc 600 gcgtcttcca tccgtacacg gttgcaacaa ctttaccagt aacattgcat ttcgtataaa 660 aaaagatttg cagcaacata tacttgagag aaaaatgtgc attttaatat cgtcagcgg 719 // ID FN619649; SV 1; linear; mRNA; EST; INV; 697 BP. XX AC FN619649; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-697 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 17f82204d7c3224331f7ba88cc729564. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..697 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 697 BP; 258 A; 98 C; 135 G; 205 T; 1 other; taaattttag caacgttcac accaccaaag atggaaaaac gtagatttct tgtaaatcta 60 acaaaagata aaagaattac tcgcgtcttt gagagatgtt tctcagatgc cactgcaact 120 gataataatg agaaaacaac acattttgga ttcaaaactg tcaaggaaag tgataaagcg 180 aaagaagtat atacagtttt tgagaaagta gcaaattcgt acgatcaaat gaatgatgct 240 atgagttttg gaattcatcg catttggaaa gatatattca ttcaaaggct cggaccaact 300 cacggcacca aattgttaga ttcggctggt ggcacaggag atattacatt cagatattta 360 aattatttaa agaatacccc taattaccaa gatgtaaaaa gttctgtaac agtttgtgat 420 ataaataaga atatgttaga cgttggtaaa gtaagagcaa aaaaattagg atggacagat 480 caaaataatt ttaatattga ttggaagcaa tgtgatgcag aaaagcttcc tttcgaggat 540 gattcgtata atgcttacac aatagccttt ggaataagaa atgtaacgca tattggaaag 600 gtactttctg aagcatacag agtacttact ccgggtggtt gttttatgtg tttagaattc 660 agtcacgtag ataataatat ctttgaatgn ccctatg 697 // ID FN619650; SV 1; linear; mRNA; EST; INV; 719 BP. XX AC FN619650; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-719 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; f34b038d8f4935aa6e6f5fa0fc2334bc. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..719 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 719 BP; 223 A; 171 C; 147 G; 178 T; 0 other; gaatcatatc acttctgtca cgacacgaag aaactacgat tagagaaaga atactccacg 60 aagccaagat gaaaacaatg tttgcggtag ccctcgttac ggtctttctg gcctttgttt 120 ctgccactcc agaatgtcca tccaaccaag atgatgttgt cttggttccg aatccagctg 180 attgcgcatc ctattatgcc tgcgacggtg gcgtagcata cttaatgaat tgctctgccg 240 gacttctctt taaccctgaa ttacgagttt gcgattgggc agagaatgta acctgttcca 300 ttaactcttc atccagcgcg gcacccagag gagaatccag cgaagaatcc agcgaagaat 360 ccagcgaaga atccagcgaa gaacctagcg aagaacccag cgaagagtcc agcgaatcta 420 gcaaatccag cgaatccagc gaatccagcg aatccagcga atccagcgaa tctagcaaat 480 ccagcgaatc ctgcgaatct agcaaatcca gcgaatccag cgaatccaat gaagaagacg 540 atgaactacc tatttataag taaagaatca gaagtttgaa gatatctcag acggaagaca 600 aattgttact atttatacgg gatgtctcag aatcactctt ttcgtctaaa attgtcccat 660 ctcgaaattc tgtacacatg ttttgagcta ttgcacatta ttatgtatta tagtgatcg 719 // ID FN619651; SV 1; linear; mRNA; EST; INV; 680 BP. XX AC FN619651; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-680 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ce1ca025d7fa3f30574137b41f7e85bd. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..680 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 680 BP; 276 A; 106 C; 110 G; 187 T; 1 other; ttcatgacag tcaagaggtt acaacgaaat aacagtgtgc ccgctaataa attttattta 60 gaagtgataa tatcatgaat acaaatgaac gcatataact ttttgtgaaa taaaaaaagg 120 atattcaagt tgaaaaacgt acaggaatcg agatagtata tcgtcggttc agaacataat 180 gcatgaataa taaggaaata caaattttat taaaaataac gatttttggg ggaaaatttc 240 cttaactcaa tatccaaaag gaaagattac tttatcagaa aatgtagtat tcattaaata 300 tgatgttaat gaaaatataa aatttatatt atgatacttc tatcatagaa aaaaagatag 360 cgaagagata cttcttttga acggcatttg aatttcgagg aaaatgccaa tactttcaaa 420 tttatctcat cgttttcata tgttaacaac cgatgcgaaa cctgatccac cacgaatacc 480 gatcgaagga tatactcaca atttagaacc aaatacaata aacggtgatg aaacgaagac 540 gaccatattc gaatctgttc caattactac agaaccgcaa ttggaattaa agaacgtaac 600 gtccccggaa aaagacgacg cagtacctat tacgaatgcc nagtcttttc tacgaaaaca 660 gatctgaatg atacgaaagg 680 // ID FN619652; SV 1; linear; mRNA; EST; INV; 672 BP. XX AC FN619652; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-672 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 93cc3c06b5ae60270526ca91ee3037d9. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..672 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 672 BP; 254 A; 132 C; 151 G; 135 T; 0 other; gtctggtgtc cggattatac agactccagt gcagacatac aacggtacac gcatgagtca 60 tacagaacac acacacacac atgtcgagac attagataac ataaacatat acatacacat 120 acacgcagtc gaaggaaaag atatagaata cacggaaaat atacggaata tgatagctcc 180 cggtatctgt ctcgcgttac agatccacac gcacacacac acacacagag aacacacatg 240 cattcatttc tacatacaca tagagatatt ccatcatgaa agacgaacgg tacaagagac 300 ggtgtgcacg cgtacgtaat tacgttacgt ttgagtgacg gccgagttct aacacaattg 360 catattaggt atgtattata cgaatgcata ttaggtattt atatagaatc tatataactg 420 tataattgta acgaaatgcc aatgtccgtg gacccaggca actgtatcac ctgacaacgc 480 gcgatcaatc gaattgaagg tgttgcgggc ctgcagtcgc ttaggtccag cccttggacg 540 caggaagaga aaaaagaaaa agaaacacgt gacaagaaca ggaaaaaaat gggcgaaaaa 600 ggaaagaaag gtggggagaa aaaagtatat atgatacgaa cggaaaagag aaggctagcg 660 gaagatagag ag 672 // ID FN619653; SV 1; linear; mRNA; EST; INV; 658 BP. XX AC FN619653; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-658 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; fa7d794f7e453f97ccdd4e32962dcaaf. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..658 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 658 BP; 192 A; 140 C; 148 G; 178 T; 0 other; accgataccg gctgcgacaa gtgtaagttg agctctgcaa ctttgaaaca gttttacagc 60 aagcaagtga agacgccgtt tgtcgctatt tgtcttacat acacacctgt gtcaatttaa 120 agatgttaaa agcactgttc ctgattgctt ttatggcgta tgccactgcg aacaacgcga 180 ctgataagaa aatcgtttgc tactatggta gctggtcagc ttatcgtcct ggacttagaa 240 gattcgaacc aaccgacata gatcccactc tatgcactca tataatctat actttcattg 300 gtatctctga gaatggtaac atcagaatat tggataagtg gatggatcta ccaaacggta 360 gagatggtta tggaaagttc accagacttc gtcacctgaa tccagaagtg aaagctttga 420 tagcgatagg tggttggaac gaaggttcct ccaagtattc caagattttc gcgaatccta 480 taaaacgcgc gcgattcatc cgcagcgtga ttacgtttct gcagatgtac gatttcgacg 540 gtttcgacgt cgactgggaa tatccgaacc aacgtggcgg caatccaatc gacgtacaga 600 actatgtcac gttgttgaag gagctgagag aagaattcga caaacacgat tacattct 658 // ID FN619654; SV 1; linear; mRNA; EST; INV; 646 BP. XX AC FN619654; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M23 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-646 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 1127dd958c858d3f88d00c0159281a48. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..646 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M23" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 646 BP; 255 A; 102 C; 113 G; 176 T; 0 other; gggggatcgg ccccctaagt gatgtatgcc gtatgccaac gtcaatcgct atctatgact 60 atcgtatttc tcaaaaaatg aatcaaatgt ccagagaaga aattgatatc agacgacttt 120 taacaagatg tgaattcatg gcaaaagatg atcctcataa ggattggaag cttgaaaagt 180 acatccttgc tttggatgat atgatcaaac aattacaaac attaccacgt aaaccatcta 240 aggataccat ggtgggttat attaaacgaa tagatttcct gaagggatta gtaaatacaa 300 ctaaacttac aaatccagta gatagagttg cagctgtaca aatgttacca aaaagttcta 360 caacgtttaa tgattcaata ggtccaaata taacaaccca gatacatcaa aaaacttcag 420 caaaatataa tagagaattg cgtgctgaat tattccatag tgataaagat ggtataagac 480 aaagattaac tagtacaaac atgcatgatg atgatttagg tgcaatttta aaatataata 540 gaaatattca agaaaaaatt gctgagaata tgctttccat gactagtagc ataaaagagc 600 atgctttagc agcaaatgcc attataaaaa aagatattgg cttact 646 // ID FN619655; SV 1; linear; mRNA; EST; INV; 671 BP. XX AC FN619655; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050M24 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-671 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; fa2c4b699b5691b8e8c2cebd772035ca. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..671 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050M24" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 671 BP; 199 A; 131 C; 170 G; 170 T; 1 other; acggtactcg ttcgtttcaa tcttcgattg attcgttaga atattttgtg gcaagtacga 60 cgttgaaaaa tttattgttt tcgtttttgt gatatctatt atctaggaag aaggatacgt 120 taatttataa ttaatagaga aaagatatgt caactgtaaa aatagagatg cgggctattg 180 atgttctgcc gagatacgct agcggatgtc gtcttaagat acggagggaa aaagagagaa 240 cagaaaaaca aacggagtaa tcaagatgac ggacgagttg ttgttctccg cgttgggcct 300 gaccatggac acaggtgtcg agaagcaact gttctccacg aattccacga gtttcgcatg 360 cgactacgag gcatcctctt gcagaacgac gccacgcagc gatgactcgg agactgtcga 420 tcaatcgaat aatataggaa acacgatcga gtgttacacg gatctgactt gtccggcgaa 480 gacatcttgg aacgaatggt ctgataacaa ttcaacgcca ctgtcaggca caggatgtct 540 aagcgaacta agcgaactga gcgagttaga ctcgaagtta gaatggtgtt tagacagaag 600 ctggaactcg ggtcttccgg acaggacacc gttgtgcaca ccangatgcg aggggtttct 660 acaccttcct c 671 // ID FN619656; SV 1; linear; mRNA; EST; INV; 652 BP. XX AC FN619656; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-652 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 4360fc90ed64f5f51a3adf350f3efdb2. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..652 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 652 BP; 216 A; 122 C; 105 G; 208 T; 1 other; attatttatc gcaattcgat caactacgct cgcctcgttc gataacgcga acgccataca 60 ccagcgttgg tccgtaaacc aatgacagta agtaatttcg tctatcgcgc cataatccta 120 tttagttacg ttaaattatt tgtagaacgt tgaaaagaga aacgatcatt attctataat 180 taacgcgctg aaagctatac tttgattgta attcacgatt gtattcccgc tactaacgta 240 tgacacgtct ggtcaagtaa tgcatttaaa aggtagaaaa atcgaccggt atctcgcaca 300 ctcgctatat caaacaatac cggttatttt atataacata acaaagatac tttacaacga 360 gataatattt tataccgctt attagttgat cagctattcg tatcgttgta acaacgcctt 420 cttagctttt tttctaggga ctttcccgat tttaactacg atgcataaat ttaatttaaa 480 atttagataa tacgtatttt ctaagaaaca gaaagctaat atgtaaaaca ctaggccttt 540 tgaaacacgt tataagcaat taattatcat acggtgatag taaatgcgtt caaaaggata 600 gcgatcgaca atgtgcctta tacgtgtagc gcgtgtaanc agaatttttg ca 652 // ID FN619657; SV 1; linear; mRNA; EST; INV; 719 BP. XX AC FN619657; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-719 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 4b27f2fedcc1b913ad80befb84f90e06. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..719 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 719 BP; 198 A; 162 C; 208 G; 149 T; 2 other; cgatcgattt ggagggctct tttgaacgtt cccgaaaacg gccggagaaa ggacagaaag 60 gtaacgccgg caacaaggga aaggaacgat tcgagagatc gcaatgcgga cgaaactgca 120 gttcctttta tttgcgttgc tgttagtgtc ccttttggcg gacgtctcag cccaacaacg 180 acgaaaaaaa catcgccgtc gcacaaccac gacggaagcc accgttcaag atgaggtcat 240 gaacatgctt gaggctgatg aaatagccga ggcagcggag gatgctgagg tcaccgtcga 300 agatggcaga aacgaggttg gatcgaagcg tcagagcttg ctccgtatgg atccgtgcat 360 ggataaacac tgcggcgcag gacgagtttg caaggccatc gacgaagaca ccgccgagtg 420 tgtttgcgtt gaggtatgcg acgaagaggt cgatcctagg cgaaaagttt gcactaatca 480 caatgaaact ttcggaagcg attgcgaggt ttaccaggcg cgttgtttct gcgactccgg 540 cgacgccaga tgtagaaacg ccgaanagta tcaacacgtt cacgtcgtat acttcggtga 600 atgccgagag atgcctgagt gcaaaaagga agacatggct gacttcccag gcgaatgcga 660 gactggctgt ttatatcatg agagatcttg ctgatcgtnc agaatactat cgcattatc 719 // ID FN619658; SV 1; linear; mRNA; EST; INV; 632 BP. XX AC FN619658; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N03 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-632 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ca3f7f49f7a7b3ea2f046ec018873a12. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..632 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N03" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 632 BP; 219 A; 102 C; 108 G; 203 T; 0 other; cgagaacttt cgtgttgtac tatatgctta aaatcaatca gaagcactgt gaaacaaaac 60 aattgtttac gaggaaaaga ccaattgtat tttgttcgct ttcatattct tgtttgcatg 120 cagtaatgtc agacatgtga aaacaaagca tagaaaacga cctacgaatg atattaactt 180 gttaacaaaa ttctcggaga tgctaaccta cattcagtag aaaatgagta acactggtag 240 cgcttcgtgt tttattccgg atctcaaatc atcatacccc gatgaagata acacgttggt 300 gcgaaatttg tcaggaatga atttaccgga gaaaatttta ttcgtaatcg atacgacaag 360 ggagaggaat tgtactcctt ttaaactttc tacaggagct agctatatgc ctttgtttat 420 gataaaacga actattgaga attttattta cattaaatct atcgttcaac atagtcatga 480 atatgcatta atgattttga attctcacag ctctcaatgg atatgcgatt ttaccaataa 540 cactaacagc attgttaatc acttgaatct tattaatgat gatcttctag aagaagataa 600 aaaatcatac gattttggac aaatgtttga tg 632 // ID FN619659; SV 1; linear; mRNA; EST; INV; 743 BP. XX AC FN619659; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-743 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8a310698e8af518f0cb5359e957eaa69. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..743 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 743 BP; 283 A; 122 C; 121 G; 217 T; 0 other; tattatcgcc aatttgttct gttccaacac aatatatctt cgtaaggttg cagtcgtaag 60 gttacacctt tcatacttgc atttctaaaa tggagtagta attattttat aaacttccaa 120 ttgatcgtaa aaaaaaatca ttataatatg tattttgcat ttgatacttg aaattttcga 180 actatcaaat aacacaagac aatcgtaatt gcataatgga aaattaaagt tcatagtaat 240 atattaataa attttatgtc tatcgaatat ttctagagat taagatgatt ttaatttctc 300 cgatttcctt cagcagcaaa aaccatagaa cttttgatcg acctaatgac aaaacaagaa 360 caatggaata tccaagctac gcggaatagc ataaaatggt tttaggtgta ttccaaataa 420 gttcgaaaat aatatcgtat gattaaaaat aaagcgagag aagccaacga tgctctatac 480 gtgggtaacg agcaggaatt acaaaaagca actttaagag atccgcgcaa gtaacgagag 540 aacgaacctg agcgcatcag aatgcaatag taattacagc cgcagccctc cggcgctgat 600 ggaagtagtt ggaaaataca agtgcaaaac aatttaagta tctgcgtgca aattcaaaag 660 ataataattt atatactggc aaatacccaa agatatgtgc tttcgctttt ctataaataa 720 tcacgacgaa tgtatacaac agc 743 // ID FN619660; SV 1; linear; mRNA; EST; INV; 715 BP. XX AC FN619660; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-715 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8068ff874c56bbb881bad246448963cb. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..715 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 715 BP; 197 A; 136 C; 156 G; 226 T; 0 other; cgaatacgaa tattcgagct cgcagccacg cttctgttac atgttaggcc aacaaggagc 60 caatcatagg ttctcaacga gttttgtaaa cgaaactcta aatgctgcca acacatgcgt 120 accggtggaa ttgaactcaa aagtattcat taattctatc gtaattgcgg ttaccggtgt 180 aataggatat actttggcag gcacgctcat tactgtagta gggaaaaaga aacttatagc 240 aacttgtttc attgtagcag ctgcttgttg tggctcgctt tactgggccg aagacactaa 300 cagtattctt ggtttgtgct cagtttttgt agccatgtct agtataggtg gtgcctgtgt 360 cgtaaacgta atcgttgata atttccctac atgtttaagg actatggcag ttagtctaac 420 tatgatggta ggcagaatcg gggcggtcat aggcaattta ctatttccag tgctgtttga 480 cttgtcctgc ttgggtcctt tcattatgat tggctctgcc tctctagtat cggcattact 540 agtgatcatc cttcaacgaa aaaaatcgca gaagaataag ccaaagcaaa ttgcctgacg 600 aaaatgtgta tggttaaaat gtaaccagct aatagtagct aaaagaatcg cttgtaaata 660 cacactttgt gctggtcgtt gatcaattgt aatttttcgt gcatgatttt tcatc 715 // ID FN619661; SV 1; linear; mRNA; EST; INV; 764 BP. XX AC FN619661; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-764 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 353e91aae12082280339ffcd4b411e69. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..764 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 764 BP; 236 A; 125 C; 138 G; 264 T; 1 other; gagtacacat tggtattgga acgatacctc gattggaatg gtcttctggt agaggcggat 60 cctttgaact tcagtaaaat gttgagaaaa aacagaaagg cttatctaac acctacgtgc 120 cttgccactg agccatttcc ctccgtgaat tccttcttga tggccgataa tgttggacgt 180 cttcacgagc caaatgcttc tgatagccat ttacctaatt ctcctgatgt tgcccacagt 240 ggagtccaca tctcagttca atgttttcca tttatagatt taatggctgc tcttaatgtg 300 actacagtaa attattttag tcttgacatt gaggggtatg agcttcaagt attaaaaacg 360 attcctttcg acatgataaa tatcgagaca ctttctgtgg aattctctca tgttgaagaa 420 ggcaaaaagg agttgatcgg ttttatggag tcaaagggtt attatgtgta ttcaatggtt 480 attagatcag ataatttagc acacgatata atattcgtga aatacgacga anagtttaat 540 tcactaatag aataatctcg ccttagctac gttagcgttt ttcttttaat atcatattta 600 agtaacttat ttaattggaa aactacatca ttaagttata cattatcaca ttacagtaca 660 tagcataaaa cgtatttcat tgtaaatgta tttttccatt gtaaatgcaa gtggtagtat 720 aaaagctagt tgatcaatat tatgaatatt gtcgaattta ttta 764 // ID FN619662; SV 1; linear; mRNA; EST; INV; 618 BP. XX AC FN619662; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-618 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 1601779b7507e35af7677dd5ce484258. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..618 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 618 BP; 220 A; 117 C; 135 G; 146 T; 0 other; cggtatacgt attaactgtg cttgttatgt gttgctgaga aacgtttcgt ttcgtttcgt 60 ttctcaagga gattatttgc actaataatt gaaaaatgga ggataaaact gggaatagct 120 cagtggagag tgcggaaaac accagttcag tgaagaagat agcgaggccg aagtctacaa 180 aatcgcaacg gccaaaatct tcgcacccac caacttctga aatggtaaac gctgctatta 240 aggaactaaa ggatcgtaag ggatcatctc ttcaagcaat taagaaatat attgcatcga 300 cgtacaaggt tgatggagag aaattggcac catttattaa aagatatttg aagtcagccg 360 ttacctctgg cgctgttgtt cagacaaaag gtaaaggtgc ttctggatca tttaaactgt 420 ctacaattaa aagctccgag tcaaagaaga tacatcgaac tgtcaaagta tcgaaactga 480 agaaacccaa gaaatccata gagaagaaaa cgtcaccgat tcgaaaacct gtgtcgccaa 540 agaagtcgaa tcccccaaag aagaccgaag atgctaagaa atcgacagct gtgaaaaaat 600 ctacggtcaa agcagctc 618 // ID FN619663; SV 1; linear; mRNA; EST; INV; 674 BP. XX AC FN619663; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-674 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 3eb28e5e39361107f8de9c81c5cbacc9. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..674 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 674 BP; 229 A; 101 C; 153 G; 191 T; 0 other; ggtggagtat tctgacaact gtacatggca tcaaagaatc agatcttggt agatgtgaaa 60 tgtttgaaga aagacaaatt ggaggagaaa gatttaattt cttctatgaa ggatcacgtg 120 ctaaaacgtg tacatttata cttcgcggag gagcagaaca atttttagaa gaaacggaaa 180 gatctttgca tgatgctatc atggttgtta gacgtttgtt taaaacaaat gcctatgtag 240 ctggtggtgg agctattgag atggaactgt cgaaaacgtt acgagattat tcccgtgtaa 300 tagcaggaaa agaacaactt ttaattggag caatagctcg tgcacttgag gttattccaa 360 gacaactatg tgataacgct ggtttcgatg caacaaatat tttgaacaaa ttgcgacaga 420 aacaacataa aggcatgcat acgtatggtg ttgatatcaa caccgaagac attggtgata 480 atatgttagc ttatgtgtgg gaacctgctg tagtaaaaat taatgcctta acagctgcaa 540 ctgaagcagc atgtcttatt ttatctgttg atggaactat aaaaaacccc aaaagtagtc 600 aggataatgg accacatgct ccaatggatc gtggcatggg aagacaatgg tataataatc 660 gctttattaa ctta 674 // ID FN619664; SV 1; linear; mRNA; EST; INV; 667 BP. XX AC FN619664; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-667 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 5a016d1460fa3a315c1f0515413122c4. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..667 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 667 BP; 256 A; 92 C; 87 G; 231 T; 1 other; tgatggaatg gatagcgatg atgacgatct acctgatctg ctggaatgaa gacattatga 60 cgctacttta aaattatcta cattaaaaaa gtaaaaaaat tcaaaaacaa atggcgacct 120 aaaaatagtg caccaccagg caatatttta gtaggccatg ctatttgtat tttcaattaa 180 atttgctaat ggcccatgtt gcaatagcaa acaaacaaat aaggtgcacg aaaaactgtc 240 aacataaaag aatacagtgg tcaagagaaa agaaacagta atattctttt taaagattct 300 ccaatccttc tttaattcgt aattcatata ttgagttacg attaacatcc ttttttttaa 360 taatgattac ttgtgtataa tataacggag ataaaataat gaaaatgact taaattattt 420 gtggaattct ttatttcagg aaatcccgtt aaaagaactt gatacataca atgtatgtat 480 ataaaaaaag aatttttatc aactagttag tgcaaacagc tttattttgt ctctttataa 540 gctcgatata tttttgttta catttacaac atgtttaatt ttcagaatta tacttaattt 600 ttcattattc tttaataagt accataanca ttacaaaata aaatatccgt gttctatact 660 atttaaa 667 // ID FN619665; SV 1; linear; mRNA; EST; INV; 710 BP. XX AC FN619665; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-710 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0f116edfb97fdb10f04c7e6faaa4d27d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..710 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 710 BP; 213 A; 113 C; 136 G; 248 T; 0 other; gggcagccga cgaatgacga ttaatgtcgt cgagttttct gtgccgtaca atcgttggta 60 ctaaacatag cctcgggaac atgagtacct ttttaatgat taatgctgaa cgctgtgata 120 tcgcgtacat aaacattttt attattataa acataaatgt gcatattata atgttaaaca 180 aaaaaaaaat gaagaagagt actattctcc tttatatgtg aagactgaat ctcgccaact 240 tatacacagg tttagcagcc gcagatacgt tcgtatacta tgtagactac agatagacta 300 tttgtacatt attaattata acaatatgtt ttacgtcggt agtgccgtat ggggacggta 360 ctattatgtt atagttcgat aacgggtgcg tgttcgtatt gtgcattttc ttttcatatc 420 gagaattaat taatcttcgg tgtttcatga tgagaaattt accagatatt tcgcgtttac 480 ttttcgtagg tgttggaata gttgcacgaa tctggtttcc tatttttctt tcttctgtat 540 tttgcgattt tcgtctctcg attcgaatcg aagaaaggat tcgatcaaga aaaggaaaaa 600 acaagaagaa aaatcgttta tttggtttct aacgattgct tgccacgttt cgcttctttt 660 tcgattatca attagatcgt tcgtgaaata aaaagggaca tttacacaca 710 // ID FN619666; SV 1; linear; mRNA; EST; INV; 605 BP. XX AC FN619666; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-605 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 693925907faacd9dce7de21c61c357e2. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..605 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 605 BP; 198 A; 95 C; 123 G; 189 T; 0 other; accttgcaag tcctttaaca aaagaaatgt tgtctctaat tctaacggaa tagtttgctc 60 ttccagaaat atgacaggac cgtaatattc gtaagtgaaa atagctcata tatcaccatt 120 caacatggta gatctcaatt taattagaga atatcttagt atacaaatta atgaaataat 180 ttgttggtgg tggttttaca tcaatgaaat ttcatggcag ttgctaatag ctgttactgc 240 gtatctttgc gtttgtattt ctctttattc aatcttgaag agagttaacc acacagcgtg 300 gcagcttccg ggtccacctg ccagaatact gttagttaca gctcatcctg atgatgaggt 360 catgtttttt ggtccactgg tgtactgggt tacaagatca aaggatagta agatttacct 420 cctctgtctt agcaatggtg gagataagaa gaggaaagaa gaattatgga aatgtgcaaa 480 actattagga attccagaag ataatgtaac tatagtgatg aatacagagt tacctgatga 540 tcgaaatgta caatggccaa cagagatagt ggcagaatgt atcttgcaac atatagagat 600 ttata 605 // ID FN619667; SV 1; linear; mRNA; EST; INV; 675 BP. XX AC FN619667; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-675 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 41aff33174b57596013097c441fb2a0c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..675 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 675 BP; 211 A; 128 C; 131 G; 205 T; 0 other; gtgtgaagtg gacgataata cttattacga tggagatctc tttgaaacaa aatgcaatcg 60 ggacctgcgg tgtatctgta tgcctgggta cataggtatg aaacaaaaag taacgttttt 120 tcctcatgtc cccgaaaaaa attaacctcg tacagattta tggttttaag ttatacagct 180 ttgcattaac tttcacacga agctctcacg ttgtaaattt accacctagg gaaacttcgt 240 aatttcagaa aactagagag attgaaattt atatgccttt aatatcagaa ccacttcgat 300 ctccaagtac aagcagaact taaatcgaaa gccttttaaa taaagtacaa attgattgtt 360 taggcgaaaa tgtcgagccc ttttgtagga aacgcaatcg caggggttgt tccattctgt 420 tcggaagtcc tttcctagtt caccggaagt gcgcacctat gttcgtccac ggtcagaatc 480 cacatagaga ttgcagcaac tttttcagat gtcgtaagtt tataagagaa atatcgtctg 540 taaattgttt ctttctaccg atggaagtac atatatagca actatgctgg ggaaaaatgc 600 accttataac gttgcttcct tttcgcagcg agcagaaaag atatcgttat tcgtaactat 660 agcaacattg agttc 675 // ID FN619668; SV 1; linear; mRNA; EST; INV; 683 BP. XX AC FN619668; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-683 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 73a310162bdf8194163e6ed217f6376c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..683 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 683 BP; 216 A; 136 C; 149 G; 182 T; 0 other; cacaacggca acgacgacga cagctgtaac agtgacgacg acggctacgt cgacacaaca 60 acggcaccgg cggttgccgt gtcgcctgaa gctgcacctg gttggataga attttgtgag 120 agacacgccc gcgcttcggc ttctgatttt gctaaggcct tttgtaccta tgtcagtttt 180 aatttaccag aaagtgcaag atcgaatctt tcccaccgtg attttttaag aaaatttgtc 240 gaaagctttt gtgaacactt tgaaagcgaa tacctacgtc gtacgattag atcaccatct 300 ttgtatgaca caaggcatat agcaagtaat gatagagatg ttaatgttgt aagccaaaac 360 aacaatgctc ctattcgtat atcatctcat gaagagttca gtgactactc tgaacatgat 420 ggagatgcaa tatcaccaaa gcctacacac aaaccatttt tccgccgctt atcctttaaa 480 gggcttaaga agggcaaaag cttcttccat aagcagcaaa gtgatgaagt ggagttatcg 540 catagtgaac atcgtagaga taagcattca aaagctaaac tttcgaagat tgtggtggaa 600 tgtagaaaag aaggcattgt taattcttta atgggagaaa tattgatgga actcaaaaat 660 gggagaatct agacttgcct tat 683 // ID FN619669; SV 1; linear; mRNA; EST; INV; 674 BP. XX AC FN619669; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N14 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-674 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e26cb370bc603245b6f40c5bfe938139. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..674 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N14" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 674 BP; 232 A; 100 C; 130 G; 212 T; 0 other; gtgtgtcatc gtttgtttgc acatagtttt tataataaat agttaaaaat gtcagaaatc 60 tcgatggcag aggcgccgca tctgcagaaa atagatttaa cgactttaaa tcttcaacaa 120 ctcacagtat tgaagcaaca attggaccaa gaactcagtg ttttccaaga ttcgttgcaa 180 acattaaaaa ttgctcaaag taaatttcaa gaatcaggat catgcctcga gaagatttct 240 ccatccatgg agggtaatga aattcttgta ccactaactg gatccatgta cgtggttgga 300 aaactggtag atactaataa tgttctaatt gatattggaa ctggttatta tgcccagaag 360 aaggtgccag atgcaaagga ttacttcgat aggagagtgg cttatgttac tgagcaaatg 420 gaaaaaattc agcaactggg tctcgagaaa ggtaaaatta gagatgcgac agtggatgta 480 atagaaatga aacttcaaaa tcaaatgccg aaggaagatt gagaacgctt ataaaacatc 540 aactttttta aattatatgt ttttacctaa tctgttctgt gcttttattg gtaatgtcat 600 taaattacat tgttctgtcc cttttgtaag ctatgataat gattacttga gattttaaag 660 aacattaatt ttat 674 // ID FN619670; SV 1; linear; mRNA; EST; INV; 642 BP. XX AC FN619670; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-642 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 3e3411ba87ab36913abebcb372fddb0b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..642 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 642 BP; 191 A; 94 C; 102 G; 255 T; 0 other; taatcttaga tgtgtcttaa gtactacacg aagtacgttc atatcataat tgtatttttt 60 tacgataaat ttgatgtgtg ttttattctt cgttgttttc ggtatcgcac atgtgaaaat 120 taggattttg acggatctga tttaatgcaa tggcgacgtt actttccatt atgttgtcca 180 cgtttctcct aaccattggt tatcgccatg ctcctgaaaa ttcttgcaac tatgcaatgc 240 cagtgttaaa aaatcaacga taggaaaaga atgatcttct tccggtgtaa gagaatttac 300 atcgttcctg gatattgtaa tgaaccggtg gaacgagttc aaatcgagcg ggctctgtta 360 tccgtataga aatcttttct cctttgattt tcgttgaatt tcatcaaaat ttttatatat 420 ttttttcaaa tagtattact gactactagt tgttatcaaa agagaaatgt tagaagtatg 480 aatttgggaa tccgaaaatt agatgtatct gttaatcttt atactcttaa atcacatacc 540 ttttttaatt ctatattcat cagatttttt aaaatgatgt tcttacatta tttatttttt 600 tttcggaaca acgaaatatt tttatatttg cgtaaataaa ga 642 // ID FN619671; SV 1; linear; mRNA; EST; INV; 638 BP. XX AC FN619671; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-638 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 9bcc7f8016bd0bf4f21c843a30a9e641. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..638 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 638 BP; 231 A; 88 C; 102 G; 217 T; 0 other; atatgtagtt gttgcgacaa attctgcgac atattgattg aaatttttgt atatttaata 60 catgtgaatt ccgtgatgat taacgaaatt gttatttcaa tttaattgtg aaaacgaaaa 120 tatggaaaat agtatttttt gtatactaaa acttcgttgt agaaatactc tgatcattaa 180 aagaacgttt tttgaaccat ttagatcaca atatccggta atatatgata aacctattga 240 taaacacaaa aaattgacct tttcacagaa attacgaaaa gtgtctgatt tatttaagag 300 taactgtaaa ttgtttattc aagaaatatg ggataactac agtatatatc catgtaactt 360 tgcagtaaat gaaattaata atatatggaa gtttgatgga actcaaaaat ccttggatca 420 atggattgta aattctgata aggattatca tcatggttat tcagctgcta aattagaact 480 atcgtcttat ggcactggaa tattccatgg tatattagat acccgtatac ccaaagacgg 540 tcgaactaca aatgctggct actgcaatat cacttcagct ccaagatata gatcttttgg 600 tcaacagtac tactatgatt ggcacatcat aatgaagt 638 // ID FN619672; SV 1; linear; mRNA; EST; INV; 674 BP. XX AC FN619672; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-674 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 6f807e84d4a0c62a2957138748a8023d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..674 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 674 BP; 178 A; 122 C; 134 G; 240 T; 0 other; ccagcgtaca gtgaatattt cgagccgcgc tctcggacat gattgcgtaa cgcatgtaaa 60 ttacaattgt tttgtcgata acgttgacgt ggtagcgcgt tcgatcaacg tcgaaccttt 120 cagtgctttt tctgcgaatc gtttatcgaa ttcacacaca gttcgtcgcg ttgtgtaacg 180 tacaacaggt taattagact gcacgctgtg gatcagaatt gtattgctct gtcggtaaaa 240 gatcgcggac tctgcgagtt agtgatgatc tttgactttg tcgtgaaata ttttttatcg 300 ctttttctca actttttata tcaacattgt ttaaatatgt atttattaag attctattga 360 taatttcctt atatagtcat atagttgagt tagcgatata taaggtagta ctaggtagat 420 aattttacat agctactgtc gtttttctaa ttttcttgta ataccaggga ttatctattt 480 acgtatatta caattggcgg aagagtattc gtgattaaaa tggcacgcaa agttctacta 540 acgttcggcc aatattatcg aaggtctaag ccgcgaacat gattgtgtaa taatgtgtcc 600 gcgtcttatg ccattattgc attcttaaac gcatcatcac gtaactgtcc ctctcgtagc 660 atcggtgtcg ttaa 674 // ID FN619673; SV 1; linear; mRNA; EST; INV; 679 BP. XX AC FN619673; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-679 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; d499cfc93a0b7fce3781ac9ffef997e2. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..679 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 679 BP; 238 A; 123 C; 129 G; 188 T; 1 other; gggcacaggg ttatttgccc atgcaattca tataatgtgt tctccggagt acagatttat 60 ccgcaatgaa cattgtgata atgtaccaat tggagcaaat cccgatagaa ttctacggag 120 aaaaagaaga aatatatctt gtccttatgg tttgaaatat caagattcat ttgattttca 180 tgaccgtata ttttcaaaac atcgacgaac cctgtcaaca tatgaacttt gccgtttggg 240 tctaagaaaa gttgaggaac caaaaaattt ggaatatgtc agttacgaat ccaggttaaa 300 ctcattcgca agatggcctg catacatcag acagaaaagt gaagaattag ccgatgctgg 360 cttctattac acggaaattg aaaatatcac tacctgtttc cattgtggag ttagaatagg 420 aaattggaga ccacaacaag atccattgga actacatgca attttgtctc ccacctgtta 480 tttcatatca acagtacagg atttcaaata tgctaatagc gtaacaggcc aggagttata 540 tgaaacttct acagaaacac caacacaaat ttcgtatggg attcctaaga gnatccatag 600 tgaaaatgat actaatgaaa tgactctcgt tcagaaaatt gctgctgcga aggaaaaaaa 660 tcgagcattg aaaatgctc 679 // ID FN619674; SV 1; linear; mRNA; EST; INV; 691 BP. XX AC FN619674; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-691 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 326e22fc4c50c43b9ab56f44fa8149d8. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..691 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 691 BP; 214 A; 122 C; 141 G; 214 T; 0 other; cttgtagttt tctgcacttg cgtcttataa catagaacat aaatcatggc cccaccatca 60 tatagtgatt taggcaaaag tgctcgtgac ttattttcca gtggatttca ttttggtcta 120 attaagttgg atgtaaaaac aaaaactaag tctggtgtag aattctctag tggtggggta 180 tctaatcaag atagtggaaa agtatttggt actttaggaa ccaaatataa tattgacgac 240 tatggattga aatttagtga aaagtggaat actgataata cacttgctac tgatgttact 300 tttgccgaca aattgctcaa aggtcttacc cttggctatg gctgtacttt ctctccacaa 360 acagggacca agactggaaa actgaagaca tcttacaaac atgagaatgt atcagccaat 420 gctgattttg atcttagtct ttctgctggt cctttggtaa atgcatcaac tgttgcagga 480 taccaaggtt ggttggctgg atatcaagct tgttttgata cacaaagaaa taagcttaca 540 aagaataact ttgcacttgg atataccgca tctgacttta ctcttcacgc agcagtgaac 600 aatggctgtg actttagtgg cctcatttat cacaaagtga aaccagaact agaaggcgca 660 attaatctgg agtggaattt cagcacatgt g 691 // ID FN619675; SV 1; linear; mRNA; EST; INV; 604 BP. XX AC FN619675; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-604 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a30c8c37fe60f480e873d0965e6f52f2. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..604 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 604 BP; 211 A; 74 C; 99 G; 219 T; 1 other; aattttttta taaggtaagg gaatgtgaat ttgcagaaga acgcatgaaa gaaaggaatt 60 aatagacgtc gtttgtatat ctcatttaaa caaatcaatg acgtacttag attatggctt 120 gcctatgatc tcttccgtaa gcatagttgt ataatctgct aacagattta taatttttgg 180 caatcttatt ctcctgtatt gctctaacaa aatatttttg ttctttcaaa tgttactttt 240 tatctaacaa ggcaatacaa agatgtaatc gttataagtt tttgcttaat gaaatttttc 300 aaaatgtgga tttaatacct catattttac atgtattgta cgcgaaaagt acattcatgc 360 tataccctaa aaattttaat tcgtttttac tgaaatgaat acgaggatac ggtaaaaata 420 tgaatttcga gtttacgtga taaaatgttg gccgtaccat gatacatgtt ctataaaaaa 480 tgttttgtga agtgataaag agggatattg aaataatggc tcataataat gaaatgatag 540 tttatgatac ttaaatgtag tctatgtgac aaancatttc tgaatatgac gatagaagtt 600 acct 604 // ID FN619676; SV 1; linear; mRNA; EST; INV; 627 BP. XX AC FN619676; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-627 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a6bedb56cb6ad42924024a9c5e897e9f. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..627 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 627 BP; 209 A; 106 C; 110 G; 201 T; 1 other; tacgttaaag acttgacgcc ttcttcagac tttttcgtta caatccgagc atcattacta 60 tgatattgtt ccatttttac cctttcatat gtccggacta gctgcgaagc aagtattttt 120 gagaaatttt tgtaaaaaca tattaacatc tgagagacta tcttgctaca atttggtggg 180 aacaatggca aattcagctg cacaacagaa attaaatcag gcatcaatgg aaccattctt 240 ggaaacatat tacgcttcta gtaataatta tgaaattcca ttaaattttt taccagaaaa 300 tttaacatgt gaaagtaaca tgcaaacagt tcctgggaca aatgcatgtg ttgaaactcc 360 aatgttttta atggataagt acatgtctga taaacagaag tataaagcaa tgagacaatg 420 gatacgtttt aagaaagaga agctgacctc taattctgaa gaatctttta ttctgagagt 480 attatccttc aatattttgg cacaatatct attggaagca tatccatttt tgtacaaggc 540 tcatgacaaa caggcactgt cttgnaagat taggaggcag cttctctttg cagagatttt 600 aggagcacaa gctaacgtga tttgtct 627 // ID FN619677; SV 1; linear; mRNA; EST; INV; 665 BP. XX AC FN619677; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N23 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-665 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a0e607cdba77a36b6f21633ce0fead75. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..665 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N23" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 665 BP; 158 A; 139 C; 147 G; 221 T; 0 other; gcggaccgaa aaacgatccg tataaggagc tggcccacta cctctctcgt gctcgttcgt 60 tcgacgtccg gtcgaacaca cacacggtac tcttgcgaat cgtttctctg cgagtaaaag 120 ctatccactt gggttccgtg tcgtctgccg tcttgcctcg tttcttctcg tgctgtttag 180 tatcaagtga gattggttca ccatgtatct gctctaaatt acgattcgaa tcttcaggtc 240 cgcgttccgt gcacgctcga aatcgcactt tctcgcctgc ggcaaggcag cgccaatggt 300 caccttaccg gtgtttaatt gtatatacgt aagctgattt aacacgtcga cgttgaccat 360 aacgtcagaa ggaaattctg ttaagcagag attggctttc gtttaagttt gataaatgta 420 agaatttatg gctagcctgt ttagcatcga atcatttttt tttctttttt tttttcgata 480 gtggagaaaa tttctggttt tgttaattat gtcggaagtt ttgctgggtg gaattgcctg 540 gtaatataat tacatatatt tcgtacaaaa gtgttacgta gtttgatacg cattcatcct 600 acgtacactc gtcgactaat gcgagaagaa gagagtggct tttcgccatt agaatagtat 660 tgtaa 665 // ID FN619678; SV 1; linear; mRNA; EST; INV; 593 BP. XX AC FN619678; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050N24 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-593 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 615a195a1c2289d702eba8fab6346846. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..593 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050N24" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 593 BP; 248 A; 117 C; 153 G; 75 T; 0 other; gcaccggtaa gtgatactcg tgagacgaaa tggcaccccg taggcctgcg aaacattcgg 60 aggcagaggt tgcagagaga gctgcagaag ccggcgatgt gccgcccccg aagaagaaaa 120 aagctgtagc gaaggctgcg aataaaagtg acgaggaaaa gcaaattagg tttccgagag 180 ctgcaaaatc aactaaaacc gagcagacag aaggtacaga aacggtcacg gcagtaacta 240 ggaacgcgaa aataaaaccc aaggcatcag ctaaaaccgc ctcagtgact gaaaaaccaa 300 actctaaaat caagtccgcg ccgaagagga acaaagaggt tgcggaaaaa ggtgatacga 360 atccagtcaa cgagaaggtg aaaaatgctg aggaaactga aacgaaaaag acacgcgcta 420 aagccacagc gaaaaaggcg ggaatcaatg tagacgaaga atttaaggga aagtcaaagg 480 gaaaagccaa aagagaacct gttaatgaaa atgctgaaaa tttagaaaca ccagctacaa 540 aaaagagaaa gcaaacaaat gctgagccca catcaaagga aagtaaacta aag 593 // ID FN619679; SV 1; linear; mRNA; EST; INV; 653 BP. XX AC FN619679; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-653 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 050e9ed89a3f9284841eab62749f4b60. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..653 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 653 BP; 203 A; 107 C; 123 G; 220 T; 0 other; aaacagttca gaagtgttaa ctgtttggaa taatcttcag cgcgacttcg attgttgcgg 60 cacaacaaac gcgtctgatt gggtcactat tggacacatg aacgcgaatg acattcctcc 120 ctcgtgttgt attgatggaa caggcgtaga accgaaaaat tgcagtctta cgtcagcagt 180 ggtacaccag ggttactata ataagttcct ttctttcgtt aaatctcatg ctatgcaact 240 tggtggagtt ggactcggca tagctttcgt tcaggcgatg ggaatctgct tctcagtttt 300 cttagctcga tcgattagaa acagttacga gtcggtctaa tatcttttca gcgatttgag 360 aacgtatcac gtgcatcatg ataaaatgaa gattagaaat taattctaaa tgaaagctat 420 aatcatagtc ccatttaaga agcgttaaat gaaaaaggta ttttacgttt ctaggattta 480 ctatcatatt ataatcaaag gagctattga atatggtatg aagattgaaa tatctacata 540 tttcctttgt tttcaataat taatgtttaa tgaaaaagtt aatcacgaca aattgtatat 600 atatatgtgt acctctcgaa atcatgtaac ttttattttg aacatttttc att 653 // ID FN619680; SV 1; linear; mRNA; EST; INV; 669 BP. XX AC FN619680; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-669 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 149b33e5ec1696d6cd3cbe405b4ef3de. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..669 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 669 BP; 214 A; 105 C; 124 G; 225 T; 1 other; atctgtttta acgttgagtc ttgtcgtcaa taatctgaat tctatggttc aataactggc 60 gaatattgta ttcgtgaata tcccgaattt caagaaattt caatctgtgt gtttggtaaa 120 agttgtattt agatatcata cccgtttcgc ttataatgct gatcacggta cgcgtgatat 180 gtacggatag atacgtatag atagggccga tctgtggaat ggatgcttaa taagtcacat 240 ttcgcgttac ccataattac ctttcccgag cccttttgta aagtttactt tcagttatta 300 tcgaacttga aatattcatc tatgacgccg ttttattaag gacatgacaa caatctgtta 360 tacacttact ataaactata tttgtgatta ctgtggtttt ggataaatca ttcatttctt 420 gattgaaaca ttataaagaa tttgatacag ttatggaatc aaatgacaga aaaaatgttt 480 atcttgcaaa aacgggtgat aattctttga attctggaat gagagacaca atgattgttg 540 gtcagacaaa tatgacacca gaatgtggtg aacctgtgat gaaacctgga aatgtgtcca 600 gtgttccaaa caatcaagca aagtggtcna atactcaaag taagaatttt ataaatcatg 660 cagtcccag 669 // ID FN619681; SV 1; linear; mRNA; EST; INV; 728 BP. XX AC FN619681; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O03 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-728 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; da4c426b41f4a7634de6e7c7b1a994b6. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..728 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O03" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 728 BP; 237 A; 143 C; 120 G; 228 T; 0 other; atcgttctct acaacaagta cagatttaaa aatcttgtat tttacaataa caaaatgatg 60 ttaaagaaaa tagtatctcc caccatcatt cataaggtta aacaactaga attactttca 120 aaatctaatc tttttgcaaa ttcttgcaaa ctgcctcaat ttggtagaac atctagtagt 180 cttgccaacg ttagacaatg tttaaattct ggtacaacta gcaatagaac caattgctta 240 acaaagttcc cagcaactac cactttaggc acacaaataa gccgcaatgc aagtacatct 300 acaggtgatc atgtacgtat gtgggacatg gaaagaattg tctcaacagc actaccaatt 360 ttgatacctg ctgctttaat agcagaaaat ccaattcttg atggtgtaat gtctgtatta 420 gttgtaatgc actcacactg gggtttggaa gctgttatta cagactatgc acgtcctagt 480 gttgttggac ctgtagtacc aaaaattctg cactttacat tgcttatgat ctcagcagct 540 acactgtgtg gtctgtttgt actcataaat aatggtcctg gggtttcaag agctgtgaaa 600 gatgcttggg caattggcaa aacaccccca caaaccatac ctgcctctaa tgaagaataa 660 ttccctttat gtatattttt gatagaataa tcccctatta ctacatattt tgatagatca 720 tgaatatg 728 // ID FN619682; SV 1; linear; mRNA; EST; INV; 686 BP. XX AC FN619682; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-686 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 2ca625ee42ca15f1602ecd9c929ac7a5. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..686 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 686 BP; 251 A; 103 C; 108 G; 224 T; 0 other; gttaggaaga agaaaacggc aacgttattg tgcgataagt acaattcgcc ttgattatac 60 aagtacaaat taagaattgc tccttagtca gtttgtaata taaacaacag tgcatctaaa 120 cgttataata taacataata ttgcttccta tggctgtgat tactgggtca tttgatgttg 180 actttaagag ggagtaatat catatgaaaa ctcatacttc acgccatatt tatttattct 240 tgttcatcgt taccgatata tcattatttg acgtcataaa atgtcactga ttcttatgta 300 tgcaaatata tatatttttt atgatagaat cttttttcaa atgcgggaaa aagagttagg 360 ctacgttgaa aattctttat aaatgtagct ttttcttaaa tcaatgggcg tatattcaaa 420 ttttaatcac tcttttgtat aaactactaa agcacataac aaaattaaaa tatgtcaacc 480 agaggaggaa tctgtgatgc tcgttgccaa gcatttcaca ctgcaatgat gcaaaaagaa 540 aaaaacacac gtattagatg gtttttgaag aaatcacaca aattattaga tcatttgaga 600 aaaacggagg aaattaaact tccgtcgaaa catgaaccag aaaaacagcc agtcgtggat 660 attatacatt taaaaccttt tacaat 686 // ID FN619683; SV 1; linear; mRNA; EST; INV; 681 BP. XX AC FN619683; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-681 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8774d2418ddbfe24fe3318699b6b345e. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..681 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 681 BP; 276 A; 74 C; 115 G; 216 T; 0 other; aatgttggag gaatgaattg aagagatctt tggtggatga attttatggc aaattagaag 60 tagaaatttc taaatatagt acagatgaac aaaaagaaga aaagaaagta tcttcatctc 120 agaaaaaaga agttactaag aatgagtatg atgaggatat ggaaaaaaac attataaaac 180 aattatctga atttaataat ccatttttat taaaagcttt agaagaatat ctcccttctg 240 aattgttaaa aaaaagtaat actgatgatg ctgaggtttc caagattcaa aatatcaaaa 300 tggaaactaa tattttcaaa aggcttgaaa gagaatcaaa ttatatatta ccattcagaa 360 aaatattaga aaatattcta gcagaaattt tgatttcacg atataatggt gccagcaaat 420 tagtcgaaaa tattatgtgt gaggaatata aattggaatc acatttaaca ttaatgagat 480 ctgtctatat gatggaagca ggtcatatta tgaacaaatt ttaccaaaga ttgtttcatg 540 agattgaaac taatcaaatg tggaataatt catattttct atcatgtata cttgaagaag 600 tcctttctca atggtgggca gatttaagtt cacgttggtc tattacagtt tctagcactc 660 atacaaatca agtattaatg g 681 // ID FN619684; SV 1; linear; mRNA; EST; INV; 713 BP. XX AC FN619684; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-713 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; bb4d9d80c3676df7b0264b3b3c028e6d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..713 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 713 BP; 191 A; 172 C; 171 G; 178 T; 1 other; atcatcgaat gtctctcaag tgaccattct cgttaccctg cttgcagcga ctgccaattc 60 aaaagcctat cgtggcttta ccgtgccatt gttcgatagc cgcatagtcg gaggacacga 120 agctaccgct gggcaatatc catggcaagt ctctttgcaa tggggatggc tgtttggcta 180 ctcccacttt tgcggaggag ctatcctgaa taatcaatgg atcgtcacgg ctggacactg 240 tgttctggct gtgccaacct atggtgattt tatcgtgaaa gctggaaaac acaatttgaa 300 gctggcagaa agcactgaac aaagtatcca agtcgtgaag acgtttgtac acgaaaaata 360 tacgggagat gtggctccat atgacatcgc attgttaaaa ctggcatcgc ctctgaaact 420 gaacaacgtg gttaaagcga ttagtctgac aaggtcgaac tcagtatcgg gcaaagcggt 480 cttgacaggt tggggatcaa catctaggac ctctaatcca aacatgcctg acaaacttca 540 aactgccgag ttgccaatca tcgatctgca gacttgcaaa gattccatag agaaactcac 600 tggtccatct cctttgcacg agacgaacat ttgcactgga ccgctcactg gcgggttttc 660 agcgtgtaat ggcgactctg gcggtcattg atcctcganc gtagcaaacc tga 713 // ID FN619685; SV 1; linear; mRNA; EST; INV; 695 BP. XX AC FN619685; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-695 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 516d726abbbfeab5de3e4bbf06177a18. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..695 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 695 BP; 237 A; 158 C; 114 G; 185 T; 1 other; tatatcgttg agtgtaatat ttcaatttcc atcactctcg gtttagtacg catttacgta 60 tcgttatacg tattacaaat gcaatgggag gaacgaatct ccatacacct tcatccatat 120 ttcaacatat gatgaatcag caaaatactt cacaacaacc agagacatgg atgcaaatac 180 cacaggtgcc tccaatgtct ataccatgga gtgtaccatc tctccaacaa ccagaattag 240 tcagatttac cggaggacca agtttcgaat caattcttca tcagaagaga aaacttgaga 300 cctgcgatct gctagatatg agacaaacga aacagtttat aacagaagaa aaaatggctg 360 cacattttaa aggtttacat attagttcca attatcaaca gtcttcaatg ccatcaactt 420 caacagctga ctcacaacca gttaatccta gggagttgaa tatggaacta gaattggata 480 cagctaatgc agatcaaatc aaaactggac ccaggctagt tctttcagag gaactgaaga 540 gaatccaaca tgaaccaatc cttccaaatt ccctgttatc caaattggaa aggccctcca 600 tggctttagt cctttgggaa caccgagcaa catcttcgta tatttccact agagatacac 660 cactccgatt cctatacgtc tgacgantat ataac 695 // ID FN619686; SV 1; linear; mRNA; EST; INV; 665 BP. XX AC FN619686; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-665 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 6e8fb3e70df01ceb9e6f7a1a1899765c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..665 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 665 BP; 179 A; 135 C; 135 G; 215 T; 1 other; atcttctgaa cgtaccattt aaaacataac gattaatata aaaaatcatg ggtcggttta 60 ttttgaccgc tctggtaatt ctagcgttag aaggcaattt ttaaaatata atcgtacaaa 120 gtagaaacga tcatatggag acgcctgtaa gtatcgcgag agacgtattc gcgaatcctt 180 ctgtgttcga ctacagggtc atgggaacca gcaggccatt cattattcaa atagccggct 240 aacgtagccg gatatccgct attctatcac gttcctccct ctcttcccaa actttcggta 300 tcgactgggt tatccacccg gctcgtttgt tattgcagga aacgagcgcg attctcgatt 360 cgtatgcatc gtaagtgcat gtatttacgt gttatgcgca tgtatgtatg catgtatgta 420 tgtatgtata atacgtatac ttgtatgtat gtatatatgt atgtatgtat gacgcacgta 480 tttggcactt cttatgtgat cgattattta cagagatttc gcgcgtcgaa ctcgtatcga 540 gaacgcttct cgacaagacc gggaatcgat acgaacggtt cacgcgaatt cacgctcccc 600 cctttctttt atttataatg tttatacnat gaatctggga ctgaaattta tacactgagc 660 tattt 665 // ID FN619687; SV 1; linear; mRNA; EST; INV; 681 BP. XX AC FN619687; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-681 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 637c9543b86132255c68c106577dc7e7. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..681 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 681 BP; 196 A; 130 C; 152 G; 203 T; 0 other; atagccgatt cttaataggt agacaacatt cagggaatcg acaacgatca cgaaaaattc 60 gtgattcgga gaattcgatg agtgtgtttc acaatccttt taaatcgttc gacgacatcg 120 tttcgcactc gagattacgg acaactcgag ttgtacaacg tatacacgac gtaacacgtc 180 cgttgacagt cgatgagtag ctacgcgaaa cgcttatctg ataaagcgcg gacttcgtat 240 cgaccgtccg ggaatttttt aaacatagag cacgtcacga atttagcctg gcatgtgcat 300 gtacgtagaa cgcttcacct tttttcttta cctgtctact aagagacaaa cacgcttcag 360 cgcttgctcg tagcgtagcg aagagtagct agtgaaccat agactagtct aacgatgtaa 420 atatataata tacaaatata tatttacgta tacataattg tgcgtgtatc tgtgtctgtg 480 ctgtatgaca aaagtacatg aaattgtaac agtctaatgg gatctttggt ggtgatggtc 540 ttgtaagacg ttgagtatgt ttgtaacgat gtaacgagaa gtaacgacta aagtttgact 600 gcttttgagt aattactaac gatgccgtat cttttggtct cgtcgtagag cgacttcgct 660 gttgtcggat ctaccgagat t 681 // ID FN619688; SV 1; linear; mRNA; EST; INV; 654 BP. XX AC FN619688; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-654 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; cc1f908a99eb89cfb2889e7fd2086f81. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..654 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 654 BP; 234 A; 98 C; 112 G; 210 T; 0 other; atagaaccat atgtcaagtg tatgtaacat accaaaataa aattgacaaa caacttaaac 60 agtgaactgt tcagaacaat ttctttaaaa aaatgataca tagtcttttt attatcaact 120 cgtcaggaga tgttttcatg gaaaaacatt ggaaaagtgc agtggcacgg tcactatgtg 180 actacttctt cgatcaacgg cgaagagttt tatctcctga agacacacca cctgtaatag 240 ctacacctca tcattatctc attagtatat atcgttgtaa tatgtttttt gttgcagtat 300 gcatgacaga agttccgcca ttatttgtga tagaattttt acacagagta gtggatactt 360 ttgaagacta ttttagtgaa tgtacagaaa ctatcataaa agaaaactat gtggttgtct 420 atgaattatt agatgaaatg ttagacaatg gtttcccatt agctacagaa tctaatattt 480 tgaaggaact tattaaacca ccaaatatat taagaactat cgcaaatact gtaacaggaa 540 aatctaatgt tagtgcaaca ttaccaagtg gacaattatc taatgttcct tggaggcgta 600 ctggtgttaa atatacaaat aatgaagcat attttgatgt tgtagaagaa gttg 654 // ID FN619689; SV 1; linear; mRNA; EST; INV; 627 BP. XX AC FN619689; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-627 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b7606ea214e634dcc5f664a2ad891afe. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..627 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 627 BP; 190 A; 100 C; 140 G; 196 T; 1 other; accaaatgac atttcaagtg ttttaaacta ataagaaaca tggcgcttct gaataaagca 60 gattggtata ctccagctcc tttgtggcaa aatggtcctg cacctggtgc attttatcat 120 ttcccgggag gttctacaca ttccggtgtc ggaggattac aaagatcaca ggcccctatg 180 acaactggta catctgtagt tggaatccaa tttaaagatg gtgttcttat agcagcagat 240 attcttggtt cctatggatc attggcgaga tttaggaatc ttgaacgtgt gatgaaagtc 300 aatgacaata ttatccttgg tgcaggagga gattatgctg attaccagtg cttgaagagc 360 tacattgaaa gaaaaattct tgaagaagaa tgtttggatg atggtttatc tttaaaacca 420 aaagctcttc actgttggtt aactcaagta atgtacaata gaagatcacg ttttgatccg 480 ttttggaata atttcattat tggtggtttg gaaggtgata aactgttttt gggtactgtg 540 gataaactag gcacaggtta tagtgaccca gtaatagcaa ctggatatgg tgcatatatg 600 gcaacaccta tcttangaaa agcttat 627 // ID FN619690; SV 1; linear; mRNA; EST; INV; 688 BP. XX AC FN619690; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-688 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b0c2369ce0616dfa9fa333f6574a38c8. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..688 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 688 BP; 270 A; 127 C; 136 G; 155 T; 0 other; agagacgctt tattgtacat tagatgaaac tgaaaattgg ctgtacgaag aaggtgaaga 60 ttgccagcga caagtttatt cagaacggtt aacacgcctt aagtcacaag gtgaaccaat 120 taaagaaaga cgaatagaat ttgaaggcag aggctacgct cttgatgaat tagcaggagc 180 tttgcaactt gctaaaaaag ggatagatct cattagatca tccaatggaa aggatgataa 240 atattcccac ctaacggaag aagaggtaaa gaaggttgaa aaagcagtgc aagaaaaatg 300 gacgtggcta gaagagaaac gcatgcttct tgccagtaca cctcgcacac aacaaccgcc 360 agttactgta gctcaaattc gtgcagagaa gcagtcactg gatagtgtcg tagtaccaat 420 tttaaataaa ccaaaaccta aagtcgatcc accaaaagag gagaaaccta aagacaaagc 480 ttctgaagct catcaaaaaa ataatcaaaa tagtcaaggc gatgctcact ctcaaaccaa 540 tcaacaacaa caacaccagg aagagaaaat ggatgttgag taacagtcat ccaattttat 600 gtgtgtttaa tattattaaa tctaccatgg ttaaaataac gaaaatatgt cattacacaa 660 gtcattttta tcatgtcaca aaagcgcc 688 // ID FN619691; SV 1; linear; mRNA; EST; INV; 670 BP. XX AC FN619691; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-670 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a976a526f4c9fa0fcef8548dc8d3597a. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 670 BP; 197 A; 139 C; 167 G; 166 T; 1 other; tctttgacag ttcacttcat acagagaaga acttaagcaa cgtaaacgag acgaaaacaa 60 gcatatcgct gtattccctt gtaaacttcg tatcttgcca cagtacatct tcaactctcg 120 agatcctatc gtaatgggcg tgatggtaga agccgggatc gtaaaagaag gaacaccgct 180 ttgcgtgccc agtaaagact tcgtcgaatt gggtatggtt acgagcatag aatacaacca 240 taagcccgtg gaatctgcga ggaaaggtca ggaagtctgc gtgaaaatcg agccaatacc 300 cggcgaggcg ccgaaaatgt tcggccgtca tttcgacgag aaagacttcg ttgtgagcaa 360 gataagcagg caaagcatag acgcctgtag ggaatacttc agggacgatc tggtgaaaac 420 tgattggcaa ctgatggtag aactgaaaaa gctcttccaa atactctagg acatactgga 480 ctgggtggaa acattgaagc tccatcgatg gttttatggg ttgacaaatg taggagcggg 540 ggctgcgatc aacgagcaca caggccacct tggattcgtt tctgatcgtt gtcgatcgtt 600 ctcccgaatt gtaatatata cgtcgaattt tctgatcttt ctgtcngtgt acttgatata 660 cgcaaataat 670 // ID FN619692; SV 1; linear; mRNA; EST; INV; 699 BP. XX AC FN619692; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O14 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-699 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8795e4fc80759def86a73ff0e25dd207. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..699 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O14" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 699 BP; 275 A; 89 C; 127 G; 208 T; 0 other; tgtgtatacg tccgtggacc gtggttatca gcttacttac tctgcccaaa agttaaccta 60 acctgaacgt agttaaatac tttatacata attgtacagt ttaaattgta ctgttaccgt 120 ttgtatttaa atatgttttc ctgaaatttg tttgttagac attctagaaa tacagtataa 180 ttattattat acaaaattaa ataatatttt tcttgaaaac tgtgaaaaaa cgtccaataa 240 aagattatat ttcactgtac gtgctggaag agtcaataat aagttattac gataaagtaa 300 aagacggata ttttttatta tcgattactc acaatacatt actcacaata tatgaaaatg 360 tataaacgta aatttgatga attagaagat aataatgtta gaaaagcacc agtaaaaaac 420 tcgttagatt cggatgaaga agatgatgat gctaacgagg ataattacaa tataatgagc 480 gatgatgaaa ttgaaggtgc cgaagatgga ccaagtgctc cagaaacaaa tgtggggttt 540 acagcattta atatgaaaga ggaattagaa gaaggacatt ttgacaaaca aggccattat 600 ctttggaaca agaaaaagaa atagagataa ttggttagac acattgatgg atgcaatcaa 660 acgagtctac agcagtgtaa gaaagaagcc aagcagtaa 699 // ID FN619693; SV 1; linear; mRNA; EST; INV; 687 BP. XX AC FN619693; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-687 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 7992389c2bf361945d6af50598467715. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..687 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 687 BP; 218 A; 99 C; 115 G; 255 T; 0 other; gacgcgcgcg caaacgctct cgctcttctc attgagcttt gagagagata tacagatata 60 ttatatgtga atggtatact tgttgaatgt aagtgatcgt gtacccaaag aaaggaaata 120 gagggtgttg ttttccatgg ttttgctagg attttgggat ttctcttcaa aatcagtgat 180 aacgatttac cagttcatcg ctcgttacaa aacgcgacta taccatatac aaatacgaca 240 aagttaatca taataccgaa tgcaagaacg ttgtcagagt atttacgcga gttttgcaca 300 aaacgtacga ataaattcgt acgttctttt cttcgtacct gttggaaatt tcgatttcat 360 ggttttgaaa tagtcgttat gacttttgcg agtcaatgct gactgtgtta acagaacttg 420 tattctgttg gaaacctgta tgtaacacga tctttatttt tatctcgaga atcacatttt 480 tctaactaat acgtttgttc aaatattaca tagatattaa gtagataata aagttgttta 540 ctatatatct ctattttatc taaaagtatt attaactgtt tattaatttg tataaatagc 600 tagtttattt atattttgaa aaataatttt cgttgaagat ttacaaaaat tcctttattt 660 agctatatta agtagtatga aaatact 687 // ID FN619694; SV 1; linear; mRNA; EST; INV; 646 BP. XX AC FN619694; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O16 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-646 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e5d0c0411d15379231759c032ddafa18. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..646 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O16" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 646 BP; 183 A; 138 C; 174 G; 151 T; 0 other; ctcatttgga ttggtcaatg gacggacaat atttacgtag caacagcgga gattatgaac 60 tgctatattg gaatcccgga gtttgtcgtc agatcccacg accgtcgatg atgagggaca 120 tggactgggc gagtcacacc tgtgtaatat ccttcgaaac gatcgggata tggccggaag 180 gagccgatgg tacagacgta aataattgta ctcgtagcgg tgattccaag ttgctcgcaa 240 ccggcgacga tttcggaaaa gtcaggctgt tctcttatcc cgcttgtcaa ccgaagtcgc 300 tgtgtcatgc gtacggtggt cattcgagtc acgttacgaa cgtatcgttc cttcaagacg 360 atacgcgact aatttccact ggcggaagtg acacgagcgt tcttcaatgg atcgtaaatt 420 actaactctg ttaacttacg aatcgagtcg agtggaaaat ggacaaacgg cgaaagtacg 480 aagcaccaca aaatagcatt cggcataacc tgccgaattt agtgaccgcg ctattctcgt 540 tgctagctga aggaggagtc gaaagaaaca aacggcgtcg gttgcgaccg tggagaggaa 600 aactcctggt ttaaagaaag gggaacagga aaaatgctgc gtatag 646 // ID FN619695; SV 1; linear; mRNA; EST; INV; 701 BP. XX AC FN619695; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-701 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 81f87602ba890780a25c845f7a9758fe. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..701 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 701 BP; 152 A; 172 C; 180 G; 197 T; 0 other; tcagccgtcg cgacgcgtgc gtcttctctt ttgttggttt tgcgaacctc acgagactcg 60 cgtgtctcgc acagcgtgac tagtcttgtc cgtgttcgcc cacctgttcg ctccagtaag 120 tcgatcgtga gtatcatgac aacgaatgtt atgttccgtg taataatcgg cgactcgtcg 180 ctttgtgcac gtgcatcatc gttgttatcg taactccgtt atcttgtgtt tccgtgtttc 240 accagggata cctgagattt cgaggggttc tcgcgagtga cctgacaacc tcacggtcca 300 ggatctgcca cgcgatcatc gcgacgcgtg tgtcgtgttt cgagcaccgt aatctcgtga 360 taggtggttt gctagccggc taaactggaa cagtgtgctt gctactggta cgttgcaata 420 gaagaagaaa caaacggtgt gaggaagaat ctgttgcaat cgtgttggaa atcagtgaac 480 caatctctcg gtgataatta gtgcgaaaca ctccttggat tatgactgtt ggatgacact 540 tggcattccg tcgtcgtagg gtcgacgcag ctttccacct ccatgatacg ctttctactc 600 gtcaagaaaa tcatcatgac tcgatcgaaa gacaccccat cgagggtccg cgtggtcgat 660 agctgggaat attcgacagc ttcgtatcga acgttaaacg t 701 // ID FN619696; SV 1; linear; mRNA; EST; INV; 684 BP. XX AC FN619696; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-684 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; dc39e7095e975935dbf43c3c4029392f. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 684 BP; 196 A; 124 C; 179 G; 185 T; 0 other; cgccgatatc tttgcaggtg tacaattgta caaaggggtg ggccacgttt ggtccgatat 60 tggcggtctg gctgaagtga aaagatctct cgtagagatt ctgcagtggc cattaaaata 120 tcctgaaata ttcaagaatg ccccgattaa gttgcaaaat ggggttttat tgtacggcat 180 gcctggcacg ggaaaaacga tgctcgccaa agcgatcgct aacgagtgcg gtgttaattt 240 aatcagtgtt aaaggtccag aattattatc aaaatatatt ggtgttagcg aggaatctgt 300 taggaacgta ttcgaaagag ctcttcgtgc caaaccgtgt gtattatttt tcgacgaatt 360 tgacagtctt accccaagac gaggccacga tagtactggc gttactgaca gagtggtaaa 420 ccaattatta acgcaaatgg atggggtgga ggacagagaa ggtgttgcag tcgtggcagc 480 gtcttcaaga ccagatttat tagatccagc actattaaga cctggacgtc tcgataaagc 540 tttatattgt ccacttccga atgaggtgga cagggaggaa atcttaattg ccctgtgtag 600 aacacagaac atagacgcgg cggggttaga ttttaaagaa ctggcagtac ttacttcagg 660 atttacgggt gcagatttaa tgct 684 // ID FN619697; SV 1; linear; mRNA; EST; INV; 695 BP. XX AC FN619697; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-695 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8f5f9d9a1558b7176815757bbdc86805. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..695 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 695 BP; 212 A; 134 C; 165 G; 183 T; 1 other; gataaagatt tctttccgga agctttaaag cgattcaatt caatcgatag acataacatt 60 caaaaatgtg aaaagtcaaa tttcgaaaag aagattgcag aagagttatt cggttgttct 120 aacttgtacg tcagaatggc atgttgacat cttaactgaa tagatattct aactaaaaat 180 attgctgcag gaactaacga actaatttcg attaatttca ttgaaaattg attcgtccaa 240 tgatagtaca cattcggtgg gatctctccc taagagaaac gaaatcgcaa accagtttga 300 tacttggaag ctaaatttac gcgctgataa tcgccaacag atgcgataat taaaacgaag 360 aaacgatcga taaagattta ctgtatttcg acgtggcacc gattttcgtc gattccaagc 420 agctgcatcc acaattccaa ggtgtggaag aagattcacg agctatcagg gacgggcacg 480 ggggttcctc gagttatcgc ggccgaaccg attttgacgg ggatgataag tgtgttggag 540 cgcggctgga gtttgtattt gtcgcctgaa caccttccgg aaatcgttcc tgtcgttcgt 600 tggcgaanaa ggggaggcaa gcgctcgaaa tcgaggcgaa cgtacccaga gatgggaata 660 tttcgtggaa cgttcgccct gaccagtgtc catgc 695 // ID FN619698; SV 1; linear; mRNA; EST; INV; 718 BP. XX AC FN619698; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-718 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a9d7032f8b3c6f47ebbcc1f37bca48a5. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..718 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 718 BP; 229 A; 129 C; 135 G; 224 T; 1 other; gggggatgac ctctggtgtg tgtcgaacat gcgtaacatt gtcgagtgtg acaccgtaat 60 tagtttttga tacgtttctc ataattgttg gtattgactt tatttgaata tacggaactg 120 cttcgtgaaa actaatgctt tgtggtgaac actatcgggt aacaacaatt tgttctcttc 180 gagaaagaaa cgaaacgatt caactctcgt ttgccataac caataatttt aacacatact 240 agtggctcca tttattggaa aacgcgtagc ccattgttat tgacagtgaa gttgagttag 300 tggacttcac ccactaacgc catttctttg aaaagcgttg aaactgttta tacatcatta 360 tacagcgaag acatagacag cgaagtaaat tattaaaata aagaaaaaat gttttcaagc 420 ttgcattttg aagttaagat gacaaattat tttctgcaca gtatattcat ataattacaa 480 tacgactgaa agttcaaaat gtatgaacac ttgttcaaga gtcctttaca ccgtgtcttc 540 gtatacggaa cactaaagcg aggagaaccc aaccacaagt tgatccaaga cgttgcaaat 600 ggatacgcaa agttcttggg acttggaagg accactgttc catatccgct aataattgcc 660 actaatttat atataccatt tttgttgaag aaacctgnct ttggtcacca tgtatttg 718 // ID FN619699; SV 1; linear; mRNA; EST; INV; 661 BP. XX AC FN619699; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-661 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e60ed6c9b7cd1ef9e1ad72e2bdc4026c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..661 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 661 BP; 237 A; 99 C; 114 G; 211 T; 0 other; cagttggcat cattagtact aaaatggtgc tatagtcgga gacattatga tacgtattta 60 attgcagatc gatcaaaaac aaattttact aagcgtacat attttcgaaa ataaatgtga 120 tcctttatca acgtacagaa tgcagatagt gcatttttct tggggtctca tttggtatat 180 tttatcaact tctcaagtta atgctgatta tagtattact ttgacacatg atggccctgt 240 agtattaggt ggaactatta catttaaagc tgatatcttt gataatggta taagaccatc 300 agaaacattt agatataaat ggagtgacaa tgcattaatt cctcatatat acgagacagg 360 ggctatatca aatacaacaa catattggaa tttaaatatt gcccatgaaa attatacaat 420 tggaaaatat gaagttgaag taatggttta taaatatgtc gtgttctggt ggcaacatgt 480 taccagtgcc cgtaccgagt tttatgtaac tgaatctttc aatggagaca tcaacattat 540 acagcccaac aaaacatcaa tggatacata tgtctcttca gcgtctgaaa gcaacatgac 600 aataaatata aaaaaaggag atatggatta tttgatgaaa gccacatcag tatcaattta 660 t 661 // ID FN619700; SV 1; linear; mRNA; EST; INV; 633 BP. XX AC FN619700; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050O22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-633 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 2a1590b5f46fbb121a28eb0553b73e03. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..633 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050O22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 633 BP; 208 A; 92 C; 145 G; 187 T; 1 other; tacgtgaccg cacccgtttt atgtggttat gtgtggtgat tcatagatat tgtatgtaaa 60 atttattgaa ttcctaaggt aaaaaatata atacaaaatg acagaagttc ataacttagg 120 tgttgacgct atcagttgcc atgcttggaa taaagacaaa aaagaagtgg ccatttgtcc 180 aaataacaat gagatccaag tttacaaacg cacgtcgagt ggttggaaat tgttacagaa 240 tctacaggaa catgatatgc atgttatggg tattgattgg gcaccaaata ccaatcgaat 300 agtaacatgc tccgcagata agaatgcata cgtttggaca caggaagggg atgggaagtg 360 gaatcccgca tgggtacttt taagaataaa tcgtgcagca acatgtgtaa aatggtcgcc 420 attagaaaat aaatttgctg ttggatctgg aggtagagta atagctgttt gttattttgt 480 ttctgaaaac aattggtggc agtgtaagca tataaaacgc ccattaaggt ccactgtaac 540 tacagtcgat tggcacccag ataataaagt tttggttgct ggatccactg attataaagt 600 tcgtgtattt agtgcatttn atagtgatat gga 633 // ID FN619701; SV 1; linear; mRNA; EST; INV; 714 BP. XX AC FN619701; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-714 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; acdf80f7620df6675989b61449d1c73a. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..714 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 714 BP; 265 A; 125 C; 160 G; 164 T; 0 other; gagttgacga tgacagttaa gtcgtctgct tgcgatgctt tagttaacct ttacgattcc 60 taggtgtaaa cgtttagatt aggttttaca tcattgcaag gtaagaaaca attacgtgat 120 gggcgataaa tcggaaaatt cgaaattgaa cgaacagatc atagaggaac tcacgaaaga 180 tctagaaagt tcatgtatca gagcaaataa caatcccgcg tcgaacgaca atatcaaaga 240 ttcgactgaa aaaaatggca attcctcggg ctcttgttac gaacgcgtgg ggattatcaa 300 tgacaatgct gatgaacgtg aaacaggggc caaaggcgac acaaattttc cgcaagacta 360 tatagacgaa gagtcgttga aagatagaga actaactctc tctgacgatg aaaaggaaat 420 ccttaaagca gaagcagtga aatataaaga taaaggaaat gatcttttta aaagcgaaga 480 atatcaagag gcaatatctg tgtacacgca aggattgcga acttgtccgt tagcttatag 540 caaagagaga tccatactgt atgctaacag ggctgccgca aagttaatat gtctggatag 600 agaatccgcg atatccgact gtacaaaggc catagaatta aaatccaact atgtgaaagc 660 atacgctaga agagccgaac tatacgaaga aacagagaaa ctagacgaag ctct 714 // ID FN619702; SV 1; linear; mRNA; EST; INV; 601 BP. XX AC FN619702; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-601 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 6ca54f212d338eac55ad706706c17068. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..601 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 601 BP; 219 A; 72 C; 110 G; 200 T; 0 other; aataatcgag atcatgaatt ggcgtataat tttgttaggt atactgattt ttaaaagagg 60 aatttgttat gaaaagtgta agccaaatat agcaattatt ggaggtggta tttgtggcgc 120 aacaagttct cattttctta cagaactatt taataataat cttaatatcg atttatacga 180 agcaaaaatt atcggtgggc gtttggccac tataaaaatt gacggtaatg aattcgaagc 240 tggaggttca gtcatacatc ctcacaataa atatatgcga aattttgttg aattactcgg 300 tttagaacac agaccatcta aaggtaaaag aacaagtatt tggaatggca atgaaattat 360 atttgaagag agtaactgga agatattatc atttattaaa ttaatttaca gatatggtgt 420 tcagccattc agcctcaaca gatacgtaac ttctgtaata aataactttg aaaaaatata 480 ccatctacag gatgatggag aagttttcat gaatatcagt agtcatttgt tatctatgaa 540 tcctgaattt ccaaagttgt tagagaaatt tattaaaagt gaaattttga aatctgggta 600 t 601 // ID FN619703; SV 1; linear; mRNA; EST; INV; 717 BP. XX AC FN619703; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P03 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-717 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 2fb6313b648b8b7fe14671458e16e3d3. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..717 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P03" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 717 BP; 226 A; 105 C; 171 G; 214 T; 1 other; gtaagcgagg tgtggttaat tggaacggat gagtgttcag tttgtattgt agtgaagata 60 tttttctttc gaataaaagc aaaaaccgat caaaacgaaa agggcacaat gcttggtcgt 120 ctacctgcat gtgtaatcga tgtgggtacg ggatatacaa agctaggctt tgcaggcaat 180 aaggaacctc agcttataat tccttctgca attgctatta aagaaactgc aaaggttgga 240 gataataatg ccagaagggt tactaaaggg gtcgaagatt tggatgctta tattggagat 300 gaagcttttg aagcgactgg ttattctgtg aaatattcgg ttagacatgg attagtggaa 360 gactgggatt taatggaaaa attcctacag taatgtattt tcaaatatct tagggcagaa 420 cctgaagatc attacttttt actcacagaa ccaccattaa atactccaga gaatcgggaa 480 tatacagcag agataatgtt tgaatcattt aatgtgccag gtctttatat tgctgtacaa 540 gcagtattag cattagctgc ctcttggaca gctaanacaa tagaggatag gacattaacg 600 ggtgttgttg tcgatagtgg agatggcgta actcacgtaa taccagtggc agaaggtttt 660 gtataggaag ttgtatagaa cacattccta tttgccgtcg tgatgtacat atttcat 717 // ID FN619704; SV 1; linear; mRNA; EST; INV; 637 BP. XX AC FN619704; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-637 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 3aa340bd289067b35e722eb0f2142ee5. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..637 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 637 BP; 227 A; 103 C; 126 G; 179 T; 2 other; ataaacgttt tactaccttt tgtctttgat atattctcat agatgaaagc ttgtaatttt 60 tattcagtac aataccatat ccaattaaaa tgatatttag tgaaattgaa atgataaaac 120 gattgagacg cagattaaag cgaacggttg acggttgact atcgatgggt aaataactaa 180 caagatcgaa ttaattaaaa ttcgatgcct ctcggcaata aacctgaatg tgtcaagtgt 240 gggactcgag agaccccact ttggcattca accgaagccg gtaatctttg caacgattgt 300 cttgaagaag aaagaaattc agaagcgggt tcaaacgtaa gaagcgaaga tgaggataag 360 ggaggtctat taaaacctac cagacgaagt acaaggatta cgagatattg caattctaaa 420 tcagttccac ataaaattac acccaagggg aaaggaagaa gaaatttgtt caaaaaaact 480 ccagttaagg cgccaacagc tacagcaact cctgttacaa gcagttatgt attttataaa 540 ggcacttatt ttncagtagg tgatatagtt tctatgcagg atattgacgg aggaatttat 600 tatgctcnaa ttcgtggttt actcacagat caatatt 637 // ID FN619705; SV 1; linear; mRNA; EST; INV; 709 BP. XX AC FN619705; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-709 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 41610bf34956399d968d1e09fc80537c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..709 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 709 BP; 237 A; 125 C; 134 G; 213 T; 0 other; ttcacatcgc cacctccaat ttggaaataa catcagctcg tgggtagatt atctattacc 60 tagatttcat cccaggacat tgaatgtcat taagcatcac aaacacgatg gagcaatgta 120 tccatttagt atctttactt atggaaaaaa tttatggaat acagaacaat tctcagacga 180 cttttctgaa agaataagag cttatgttga agagtgcgat ttaatgcaag gatttcagat 240 aattctagat tctatggatt gttttgctgg aatcggggca tcatgtatac aacacctaag 300 agatgaatat ggaaaaagta ttgtgtcatt tccatgtata gacagtaaga agactgaacc 360 ttctacatcc aacgtgatta aaatacttaa tacagcttta tgctggcaac atataggaga 420 atatgcttca ttatttagtc cactatgttg tggagaaaca gcttggccac aaattggaga 480 accgcgtata tttaatcatt tgacgtacaa ttctgaagta aagtatcata gcagtgcaat 540 tttagctact gcattggaca cattaactat tagatacagg cgaagagaat atccaaatgt 600 agttttatcc gatttatgtg cggaatctca taaactgggt cgaaaagcag tcgcaacaag 660 tctaacttta ccccgtccga tgactgctaa agcagatttg attgacgtc 709 // ID FN619706; SV 1; linear; mRNA; EST; INV; 635 BP. XX AC FN619706; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-635 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ecabd985bb9575288bf8f4f3c343460a. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..635 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 635 BP; 218 A; 96 C; 108 G; 213 T; 0 other; gctggacttc atccaacagc agaccaaata gaattatatg cctattattt gccaaataca 60 ccattgtcaa atctcattaa tatttttatt gcgttatgta aacttgatag cactctatat 120 tttatttgca atttagatga ttttaaattt cttgctgata tgatacaaca tataccatta 180 cctcttcgta cacgatatgt gttttgttgt gcaccaatca atagaaagat gcctctaacg 240 tgcagtatgt ttttaaagta tgctcgacaa tgcagtaaaa atgaaccagc aactatttta 300 tggttatgta aacaaattaa ttggcctcct agaatgccat cgaatgttgc tgatcttcta 360 catttagaaa gtatatttga tgtattggat gtttatcttt ggttaagtta tcgaatgcca 420 gacttgtttc ctggtgcaga tgcagttaga acattacaaa aagaagtaga taaaattatt 480 gagcaaggaa ttagaaaaat tacaatatta tttaagaact cgaaagcaga ttttgaggct 540 aatgaagaac ggttgtatta ctcggacaga aatctaaaaa caaatgatga aaggcggaaa 600 gatacattaa cttattcgtt gatatctcga ggtct 635 // ID FN619707; SV 1; linear; mRNA; EST; INV; 714 BP. XX AC FN619707; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-714 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b458d91ba3f12cd0d7ac1ee82091737a. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..714 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 714 BP; 261 A; 96 C; 141 G; 216 T; 0 other; actagtcgaa cgatcgaagg gttgctcgtt tttatcgaag aacaaagcta caagtcgaaa 60 gcaaagtcga acagcgtaca cgtttgtacg atatcgtaat tgcaatcttt gtccatgact 120 aaaatgaatt tcgcgtctga aatgctatga taaaatttgt atttactacg attaagcgta 180 taagtaaact gtagaggaag ggaaaagaaa gaaaatggtc gttgaaagag gagagaatgt 240 aaataggggt ctgcgaacgg tatacataat tatttagagg tttaagagaa tagaggaaaa 300 ctaaagacac acagcagtgc tgttgttaga tttatttaga ttagtttaaa gcaaaacgtg 360 gccgatatcg tataataaat gatacgttat ttgtcgaccg acaaattgca tattgtaaaa 420 cttggcaacg aataccaagt atatttaatt ttgtagcaca ctggaacgag tgtcgaatat 480 ttaccaaagg taacacgtga atacgtttgt acatagatgt tacgtgttaa ttcttgttac 540 cacgatgtaa aaatacaatg agaatgtaat agcgaatttt aaagacattc gtaattcgcg 600 atgttacgag ttcgcttcat atttatttat aatatttctc ctgattgatt tacgaacgca 660 aataatgtta aagagtttat caagtacaaa ttaaataaat ctatcgattt aaac 714 // ID FN619708; SV 1; linear; mRNA; EST; INV; 706 BP. XX AC FN619708; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-706 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 76cee775c735dfdc659d22e4fa2aea8e. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..706 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 706 BP; 214 A; 130 C; 150 G; 212 T; 0 other; ggccttttca tttagaaacg aagatggctc gtggaccgaa aaagcatttg aagcgtctta 60 acgcgcctaa agcgtggatg ttagataaac ttgggggtgt atatgctcca cgtccatcta 120 cgggacctca taaattaaga gaatcccttc ctcttgttat ttttcttcgc aataggttga 180 aatatgcatt gactaattgc gaagtaacaa agattgtaat gcaacgtttg attaaagtcg 240 atggcaaggt tagaactgac cccaactatc ctgcaggttt catggatgtt attaccatta 300 acaagactgg tgaatacttt cgattgattt atgacgttaa gggacgattt accactcata 360 gaattacagc agaagaggcc aagtacaaat tgtgtaaggt aaaacgtgtt caaactggac 420 caaaaggtat tccattcctg gtaacgcacg atggaagaac tattcgttat ccagatcctt 480 taattaaagt taatgatacc attcatttag atatagcaac aggcaaaatc ttggattcta 540 ttcgatttga ttcaggtaat ctctgtatga ttactggagg gagaaattta ggacgtgtag 600 gcacagttgt tagccgcgag cgtcatcccg gttccttcga tattttgcac attaaagact 660 cacaagggca tacttttcgc acagggttga acacgttttt atcatt 706 // ID FN619709; SV 1; linear; mRNA; EST; INV; 623 BP. XX AC FN619709; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-623 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; abc12ad4e8f8bdda74ed38fa0a44c439. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..623 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 623 BP; 234 A; 104 C; 85 G; 199 T; 1 other; acatttttat aaaagtttta gccagattta tctgcaattg gccatattac gaaatacaat 60 tctataatta aaataatgag tttctttatg atgcagaaat cccataaatt aattctgttt 120 atatggctcg tatctgttct aaccttatct atcagtgatg aaattaaaac agatggtgat 180 acgactttaa cgttaacgtt aaatgcaagc tttactgatg gagattccaa aactgaacat 240 cgaactttat cttcgccaat tcaatttaca tctgaatctg ttaattctgc taaaacccaa 300 aacaaaaatg ttggcatccc tttagttgca ggggaaggtg acccaatttc agttatttct 360 actaccgaac taacattttc tactgattca cataaatata taacaaatgc tactgacagt 420 ctagatgtac gagttaatgt atcaacagaa gatgataatg ttccttatgt aaaaagtaaa 480 aaaaccactt ctaaaattgt atcaaggaaa ggagcaaatt cttccattga aactatatca 540 tcccatgata cgaagacaat aattaaatct ttaaacacac aaatgttcat aatattggaa 600 agattgatan caacatgaat gaa 623 // ID FN619710; SV 1; linear; mRNA; EST; INV; 679 BP. XX AC FN619710; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-679 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 1873aaa28996c21316defb46de366208. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..679 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 679 BP; 245 A; 84 C; 101 G; 247 T; 2 other; cgattgacat ggcatagccc tgctcatgac gaagaaagtt cagtaaaagc tgatgcagag 60 tagcgttcag ccttccgata tacatacaca gcacgcttta gaatatgtcg tatataaaaa 120 atagcgtgta caaaatgaaa aatatgaaga tttattgatc attgcacagt catactataa 180 attttaatgt tttttttacg attattgata acttaaaaat taagagatag gccatcctta 240 ttgtgttatt gttagtcaaa atataattac tcttaaactt aaaaacagga aatatacttc 300 cataaagtct atactcgcaa cttcttttgc aactgttaca tttttgtata aggatcaata 360 aaaagtttat ttttcattgt taatttaaat gtaaagttta gtatgtccaa tgtaaattag 420 aacagtatgt atttcgaatg taacgtgttt ttttatgtaa tttctcctct gattgcttaa 480 tttgcaatta acttttaaat agatgaatat gattatacga taatagcggt tgttgctaaa 540 gaagttgaaa gcttgattat ttttattcaa atacaacgtg acagtgtaca gtaagtacag 600 aaatatgaat ganaataatc ttttaatttg taaaaacctt tttgatgata aaatactttt 660 acataagata tagtngtat 679 // ID FN619711; SV 1; linear; mRNA; EST; INV; 717 BP. XX AC FN619711; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-717 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 6e7d8399939caecdb456e81d990882b5. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..717 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 717 BP; 270 A; 96 C; 96 G; 254 T; 1 other; gttggtgcct tatcaatgga caaaactgcg taaacactgc ccgtgcaacg acaatgcgat 60 atattagtat ttttttatcc gtaaacaata gtatcatggt aagtagaaat acgcaaccgg 120 aaaaacaaag tctggatgct attcttgtac atacaaaaac gaaaactgtc gaagttggca 180 taaataagta ttttatatta atgttaaatt aaatcatgag tagtattaca caatttaatg 240 ttaattgtaa ttaaaactat gtatgtagta catgtaattt tttatcttat atatgtgctc 300 attgttttct ctttttgatt gtcgcagaaa tactttatcg aaattcataa atgtgttcgc 360 attctaagaa gattatgaaa tgtgattccc actcatcatt tttactactc atgatataaa 420 tactataatg aaggaaaaaa agagcatggt ctcatcctac gcatctcact tatgcattat 480 cttgttattc ttattaatgt tactgtcatt attatcatta ataaatgata tacgactatc 540 tattttaaaa gattcataag aaagacatag aattatgtac agagaacaga ttattatatt 600 tagtagaaat attcctacga atagtaatat gatatatata ataaatttag gttaatagca 660 tccacaacag tatacattta tttatantat attaatgaac aaaaaaacaa aattgct 717 // ID FN619712; SV 1; linear; mRNA; EST; INV; 646 BP. XX AC FN619712; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-646 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ae0c9c3ffe1b5188350dda5403237ff5. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..646 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 646 BP; 234 A; 86 C; 107 G; 219 T; 0 other; tcaccggatg atcgctggac gacgttctgt tacgaaggta gatccgcgac aagcgacaag 60 tgcgattctg tgtgatggag tttattcaaa tttcgagttt aagatgattc acactacaaa 120 taacgatgta caatgatctt catttttagt atgatatttg ttgaatgcca taagtgcctc 180 ctaagtctcc ttttaataaa tttattactg tcaaagaata taaatctaaa aaataacaaa 240 tgaaatatgc caattaatta tcgataaagt aataattaaa ttattaaatt tataaatatg 300 aggtataatg gatgagaaaa gagaatcatc ttattttatg tgaacgtcgc aattacatat 360 gaatcgcctt aatgtgtctc tcgcttaagg gaatgtttgt ggtattgccg cgtatgacaa 420 cattgtctaa gtaattcctt taatgtttaa tatattcagt aataatagct tttattgctg 480 aaatcgttac agaactgtgt atatattttc acgataaaaa taagtaatat caattaacta 540 taaaaagaaa aagatcttaa ggttacattt caacaaatca tcataatagg atgtcgacgg 600 atttttatat acgatattat gtaggtcata aaggaaaatt cggtca 646 // ID FN619713; SV 1; linear; mRNA; EST; INV; 696 BP. XX AC FN619713; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-696 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 18e72b166dc3ddefef1dd6d62f0efdec. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..696 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 696 BP; 248 A; 95 C; 146 G; 207 T; 0 other; ctacctaatg gtaaaaaagg actagtactt atttcgtagc attattacac tgagaaagtt 60 tcattattta ggaatgaatc gtgtcgattt gaaagtggtt ttattgggta attcagctgt 120 aggaaaaacg agtctcgtcg aacgtttcgt aaatgaaagg tttaatgaag gtctatctta 180 ccaaaactcc gcgaagtgga aggaatattg taaaataaag ttattaagat ttacactgag 240 aatcatctaa gagatgcgtc acaaaacaaa ttaacccagt tccaatttcc ttagactata 300 ggcgctgcgt ttgcagctaa gcaaatgcaa gtcaatggga aaaggcttgt catggggata 360 tgggatacag caggcagtga gaaatatgat gctatgagta ggatatatta ccgtggtgca 420 aaagctgctg ttatctgtta tgatatcaca aaatcaaata catttcaaag agcaaaattc 480 tggataaggg agcttaggga aattgaagaa ggatgtaaaa tatacatttg tgcaacaaaa 540 aatgatattt tagagcatgg tgcaattcca tctcctaata tagatgttgt aaaaacatat 600 actgctgaca ttcgagctaa attgtttata acatctagta aaactggaga aaatgttgct 660 gaattgttta atgaatttgc acagatttat gtctga 696 // ID FN619714; SV 1; linear; mRNA; EST; INV; 606 BP. XX AC FN619714; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P14 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-606 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b76b03c12754441b5c0f603fe79e36e7. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..606 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P14" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 606 BP; 146 A; 149 C; 146 G; 165 T; 0 other; ggcttcgcgt atgggatacc gcgaaacgag agataccggt caccataatg gttttcctcg 60 ataacgccac gtaactggcc ttgcttcacg cgtaccacgg tgttgtcgtg cgccataccg 120 cgaaacgaat cagctttcga cgaccgacga ttcgagctga actggtagca cgcttgcaag 180 cacataaata ttgtgagtcg aacggtacct ggtttcgaca tccggtatct gaccaagtgt 240 ggagcaacta cacgacgtgc gtcaacctgc aagatctcag ttggcagcaa ggaattagtg 300 gactgtacga agctggatac gcaatatcgt tggtagcatt gctattgtcg ttaggtattc 360 tcacatactt ccgctccctg aggtgtgcaa ggaccactct tcatatgaac ttgttcgcct 420 cgttcgctgt aaataacgca ctatggctgg tgtggtatag atgcatcgtc gcgaacacgg 480 atttactatt gaacaatgag ataacatgtc gactgctaca cattattctg cattactttc 540 tacttactaa ttacgcctgg atgctgtgcg agggttttta tctgcacacc ctgctcgtta 600 gcgctt 606 // ID FN619715; SV 1; linear; mRNA; EST; INV; 657 BP. XX AC FN619715; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-657 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b7e37e0893b18146c62707418342ccec. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..657 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 657 BP; 247 A; 110 C; 153 G; 147 T; 0 other; caaaggagat gctaagttga aaatgaattg gacgaagcaa agagccaatt ggttgtaaca 60 gacttgaaag ttcaaaatga tatacttatt gagaagagaa aggcacaaga agaaatagca 120 tctttacaga gggttataca tgaaacaatg gaggaatctt cttgttcacg caagcagcta 180 gatacagaat tggctaaatt aaaaattact ctctccaaat tacaagagga gaatgcattg 240 ataaagtcac aattaccacg agatccacct atagatggtc cacagatatc actctccaca 300 gtaacgaaaa caatagctcg aaaagtagtt tctcaactag gtgcagatgc tctatcttta 360 ggtcctgata atcttgagga aagcatgagg aaggtaaaaa gatatgcgca ggaagatgcg 420 gaagttttac gctcgctggt agtgccgctc gaggaagaga ttaaagcctt aaaggaaaaa 480 ttaagagcga cggatgacga actccagaag tgtaaagaga tacagttgca gaagaaacag 540 caggaacctg gtccctctga gtcaatatgt gatatgtgtg caaattacga agcacaatta 600 gttaagatgc aggcagctgc taaagaattc gagaagcagc aactagattc tgagcga 657 // ID FN619716; SV 1; linear; mRNA; EST; INV; 631 BP. XX AC FN619716; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P16 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-631 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 27b0516ee81b9b849e987fa2c843f61a. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..631 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P16" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 631 BP; 196 A; 98 C; 113 G; 223 T; 1 other; atatcagtca ggtcattgtt aagtttcagt aaaagattgt gattatttca gtgaaataca 60 attgtcaagt gtgaattttt taacataaca gatgtttatt gtaagaaatg tcacaaattg 120 atattcagga cttatttgat aaaagcaggt ttcccagtgg ttggcgtctt gcgcttatat 180 ttttatatac tccagttggt cttttacttg tatcactacg gttattaatt gctctacaac 240 tttggcttat tgcaatacta ttacctgatt ataatactct tcggacattt ttacatcatg 300 gatttagttt tgtttttggt ataatggtga agatcaatga gggtgaagtt agagacaaac 360 aggcaagaat tgtaattagt aataatgtct ccgtcttgga tcattttgct ttatataaag 420 ctacacaagc actaagtcct actatgtggg aactacctac ctccatgggc aatgcactag 480 gtttacaagt aatggatatg aatagcaaag aagcactaat tgccaatatg aaacagtttc 540 tttctacaac agatagcaat attgcgatac aacctgaatt tggtataacc aatagtcgtg 600 tagccttngt aaaatttcac tcttggccat t 631 // ID FN619717; SV 1; linear; mRNA; EST; INV; 639 BP. XX AC FN619717; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-639 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 71382dcbc23fc4a2c50f3830a8aec2dc. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..639 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 639 BP; 220 A; 117 C; 92 G; 209 T; 1 other; aaaactaaat atggactacc caaagaactt attcatttta ttttccataa agaattatag 60 aaatagttat tatctggtta ttttaatctg gttattatca ttacaaacct tcatagctta 120 cctatgatct gagacaaatc gttgtaattg cgacagttct gtttatccag ataatcttta 180 cttattgatt ccaagaaaat cgtgtaacga acgatattca taaataaatc gatgactaca 240 ttttgcaacg aaactgacaa acccttaaaa atccaacata ttcatacact tgttccgtga 300 gtgattttgg taaagagatt tctttaaatt taaccttaag ttcttcgata ttcctgaaac 360 gttccataac aggatcgtga tggggattgt atttctctct cgagccttcg ttaacaaaca 420 gacgaatgca aaactcatct ggacttaatt cttgtttaaa cgacgcatac acgtccaaca 480 tctccatcag tgggatcaga atgtttaatc gaaagacatt gatttttgtc aactaaaaga 540 agcaacgtcg agtaagtgaa aatagaacac gtaaaagtaa gattatacct cttcctcgct 600 gncatccatt actgaaaaac gacagttatt tatctttta 639 // ID FN619718; SV 1; linear; mRNA; EST; INV; 717 BP. XX AC FN619718; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-717 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 362ce2cea3aae7fa905032b98cd93153. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..717 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 717 BP; 248 A; 109 C; 118 G; 242 T; 0 other; gggtagtggt ataggtttgt tacatacatt gcattgtaaa gaagaaatac agcagatatc 60 ataccagctg attatgttat ttccaacatt atttcctctg cttgggatgt tgccaataga 120 aaagttgcaa taaagtcaga ccaattttct aatttacctg atgaggaaaa aatacctgtt 180 tataattcgg tatcttcttg tcaaaaacca atttcttggg gtgaatttat gaagcttaat 240 gaaatttatg gattaatagt acctagtaca agagttattt gggtttatac actgacactc 300 aatagatatt tatttcttca caatatctat acttttttat tgcacataat accagcaatt 360 atcggtgata cactggctca tctcactggt cggacaccta tattattaga tgcatacaaa 420 aaaatacata aatttagcac tgtgatacat tacttcgcag taaatgaatg gaagtttaaa 480 aatgacaatg ttataaaact ttggaacaag atgaattcta ctgatcgaca aattttctgc 540 cttaatgtag aatacttgga ctggaaggaa tacttctatt atcatataag aggtgttcgt 600 atgtatttat taaaagatcc tatggacaca gtagaagcag gactagccag atataaacag 660 ttacgtctag cgcataaata catcatttta ataatatcac tattactttt atgggct 717 // ID FN619719; SV 1; linear; mRNA; EST; INV; 728 BP. XX AC FN619719; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-728 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; dc956c9fb14f2181dd67fcefd1ddfa5c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..728 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 728 BP; 287 A; 133 C; 139 G; 169 T; 0 other; tcagtatttt tctgaattat tgaagagcac aataaaaaat tgagttcaat agaagaacaa 60 aagataaata actagtcaat gatagatgta ggaatccaag taggacttca ttcccctcga 120 gcacatttaa ttgcatgctt tgaaggcaca gaagcggcga taaaacgatt acgcaagcaa 180 aatgaggaac agagagaaca gatcgcaaca ttgaaagcaa catcaccaaa tatgaatggt 240 aataaaggag ctcgatcgcg tgacagtaat aatagtcatc atagtcgcgg gtcacctacg 300 agcactatac aagaacaaac tcctcatata tttccaccat tacagagcta ctggcatcac 360 aagtcattta ggaacggaag aaatcgacac aataaatcgg accatcagac ggaaatggat 420 tcgactatgt taccacaact ccaaaatggc aatataaaat tagatacgat gatgtttgaa 480 tctctatgtt atcgatctcg tggtcatcgt aattattatc gcgatgaaag caatcgaaaa 540 tatagaggaa acatttcaca aaaagatcgt agagatagtg aacataatca ccatcatcac 600 catcatcatc atcgacgaga ttacaaagat aggagttcta aaagtcatca aaaagaattt 660 ccagattcag cagaaggatc tagcgaagct gaatattctg cgggggggta atcataaaac 720 aaaagtag 728 // ID FN619720; SV 1; linear; mRNA; EST; INV; 648 BP. XX AC FN619720; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-648 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; c032e75f724b8fb98e05698d9221f902. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..648 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 648 BP; 260 A; 100 C; 112 G; 176 T; 0 other; gcgccgtcgt cgcgctctga aatcgtttca ggcgctttgt ttcttctttt tctttcatcg 60 agacaagaag aaaaaatata aatgaaataa agagacaaaa ggaaactatg aaagaggcat 120 caggcaaata tcgatttcct ctatctgtgt ttcctttcga tatttgaaaa cgaaataaaa 180 atttttaaaa ataaaagaaa aagaaaaacg aatgaaacgc tgtgctgccc ccgtgtgtta 240 gttgaacttt attagatgac ttttttcttc gaatgtgtga gaaaaatttt acataacgaa 300 gtaaaagaag aaaaaaaaag aaaatacaaa aaagtgtctt tcacgcatcg aaatgaaggg 360 aaaaaaacaa tttacacaca gagaatacac caaacacata cacactcaca taaacacaca 420 catacagaca ttttgatgta taatgttata tcgaacggga attgaatgtt tattgtacga 480 ggcccgcttt ttagcaagcg ttggtcagaa aaataattcc acgaggacac gagtttaata 540 taattaatat ttaagtagct tactataatt aaacgacgat tacgattaca taaaggacaa 600 agggaaaaaa caagtaaaca attagtgtgt ggaagctgtt tatacctt 648 // ID FN619721; SV 1; linear; mRNA; EST; INV; 692 BP. XX AC FN619721; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-692 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; d9dfa3e689b1a4ce16d5a5e90b9b5478. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..692 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 692 BP; 265 A; 81 C; 86 G; 259 T; 1 other; tttagaagca gaaactgctc tgtgatacga gtattcaaca gtgaggtggt tgtttattgg 60 gaatatttaa ttccatttgt gtgcaaaata ttacaaagat gtgttttatg tataaaactc 120 attcgattgt aaaaatatat ctattgtatt cttcaatata tgtattatga attattttaa 180 gtattactaa tctctaacta tccgattaac agctgtctca tttattttat ttaattcatg 240 attactttgt atagctaaaa atattctagt atttgacaat tactaaaatt tagaacacat 300 acgttagtaa catatgagaa agacgattgt agaaattgtt aacatcatca tatgaaagac 360 aaatgccgtc taaaacaaga gtgagatcat acatttaaca tttcataaga ataaatagca 420 gttttctcat attgagaaaa gaagcaatta attgtcttaa ctatgacata ttattacaaa 480 tacacgatat tataatatat actgtcgtta acaaagcttt cattatatag aaattttgaa 540 atacagtaca agatacaggt tgtttaattt cattactata taactttcat tattatataa 600 gtcatgtgaa tcaaatatat gtgtctgcgt ttaagctttt tatataatta gatattaaat 660 caacaaaatt tcaaaattna acaatttaaa at 692 // ID FN619722; SV 1; linear; mRNA; EST; INV; 611 BP. XX AC FN619722; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0050P22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-611 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 20e7b1b468e132614aaf60573b5e8378. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..611 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0050P22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 611 BP; 230 A; 71 C; 120 G; 190 T; 0 other; caggtcacag atacaagtca taagatttag aattactcat aagcacatcg gtcgaaaggg 60 gcgaaaggac aaaagcttaa tgttttaaaa tttttacatt gttcaaagaa taaaggtgag 120 taatgggtga gaatacttgg aaaatcggtg aagcagtcga gattccagaa gaggctatta 180 agatagcatt agagaatgaa gtgtcattat cagacaggtt ttatttatta gcaaaacgat 240 caccatttct tttagctggt atagttggtt taactactgt ttgtggaata ggtgcatata 300 agtggaaaac acgaacagta aaaccttcta tatttattgt acaactcaga gttgcagctc 360 aagctactgc acttggtatc atcagcttag gaatgtttta tggcatgtat aacgattata 420 taaagaagaa gaaggaataa cattcatcat ttaatgttag aaagaaataa ggagacaatt 480 tgaacattca atcatattca aagagtaaaa taaaatatgt ttatttttag taaaatggag 540 cttgaaagct atttatgatt attaaattta gatactttat gtgattatta aattctgtag 600 aagaaggcat a 611 // ID FN619723; SV 1; linear; mRNA; EST; INV; 699 BP. XX AC FN619723; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-699 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; cf4a0afe2d6521ddbbaccba49d090cdc. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..699 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 699 BP; 170 A; 125 C; 158 G; 246 T; 0 other; gtcggttcag attgaggttt gcgcctggtc accgtggtga cagttcttcg atacttgata 60 gtgacacttt cttcttttcc ttagcttgta ttaactacac cttccgttca tgttgtttaa 120 gtgatttcta ttaataaatt tgtgatggca cagacgcttg tttgtatacg gtactttctc 180 atcattggtg ctatcatcat tggtgtgtgc ggtatcatag aaagtgtttg cgctggatat 240 ttcatatacc agttgtatga atattcatcc ctcacgtcta gcaatgtatg tggttcagcg 300 ataacattgg tcgtgatggg tttgataacg tgtatgatag cttggtgcgc ttggcaattc 360 ctagatttta caaatagtgg ccaagttata attttttctg cactgcttat aattgttaca 420 attgttaata cgagtgctgg agtttgggcc cttgttagac atgaacaagt ggatttgttg 480 ccaatagcgc acctcgaaca tgtatttgag cttgctataa cggacgataa gtctcactgg 540 gatagtatgc attcaaagtt acattgttgt ggaataaacg gaccagccga ttatcgtagt 600 caaaatgcag ttccttggtc ttgttgcgat acgtcatcac ttgcaaatcc taatgataaa 660 ggcgtgtgta ctagcatgta tgcacatggt tgccagcat 699 // ID FN619724; SV 1; linear; mRNA; EST; INV; 641 BP. XX AC FN619724; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A03 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-641 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0791f06d67562e3e0f07f759137876bf. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..641 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A03" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 641 BP; 252 A; 103 C; 146 G; 140 T; 0 other; gaaataacat taaagaagtt gacaaacagg cagaggaatt ttcagaaagt ataaacgata 60 caaataaggc taataaagaa gaaagcgaag atgaagatga acctgaacaa gaaacaactc 120 ttttcgtaaa gaatattaat ttcacgacta cggaagagca gttaaaaacg tattttggca 180 aatgcggtgc tcttcattat gtctcgatag caacaaagaa ggatccaaaa aatcctggcg 240 ctaaactgtc gatgggttat ggattcgtta gatataaaag aaaagtagac gcggatcgag 300 cattaaaagt cttacaaatg acagtgctag agggaaagac tttagaactc aaacgttctg 360 aacgaacgct aacaactgat ttaaagagtg caaaaaagac atcgaaggta acggctcaaa 420 caggcacgaa aattttaata agaaacgtac cattccaggc aacggttgag gaagtaactg 480 aactattcaa agcgttcgga gagttgaaag cggtgcgact gccaaaaaaa ttggttggtg 540 tcgaaaagca cagaggattc gccttcgtag agtattatac aaaaagcgaa gccaagaaag 600 cgtttaaggc gttgtgtcaa agtactcact tgtacggtcg a 641 // ID FN619725; SV 1; linear; mRNA; EST; INV; 712 BP. XX AC FN619725; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-712 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; aab3fee27938a7d67bc682551dacf481. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..712 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 712 BP; 225 A; 131 C; 162 G; 193 T; 1 other; gcataaccgt cgtcgggcaa ggtagacttt cattcactgt ttggtactcc gaggagaagc 60 attaacggtt gctcgtgttt aagaaaactt ttaagaaagt attataaaag atgtcaggac 120 gcggtaaagg cggaaaggct aagggaaagg cgaagacccg cagtagtagg gcagggctgc 180 agttccctgt cggaagaatc catcgtcttt taagaaaagg aaattatgcg gaacgagtgg 240 gagctggagc gcctgtttat ttggctgctg tgatggaata tttggcggcc gaagtactcg 300 agttagccgg gaacgccgca agagacaaca agaagacgag gataattcca cgtcacttac 360 aattagctat tcgcaatgac gaagaattaa ataaactgct ctcgggtgta acaatcgctc 420 aaggtggtgt cctgcctaac attcaagcag tgcttttgcc gaagaaaacc ggtactggtg 480 gatctggaaa gggagacaaa acatcccagg aatattaata atcattagaa taacctttct 540 aacttaacag tactttaggg ctatatcttt gtttcttgag ccattaaaga aaaacaaaat 600 aatctctgga acctatcaaa ttaccatcgc ttcatncaac gttcattgta aattatttta 660 gttaacagta taatctacta ttttatttta ctgatacatc ttttatttag ca 712 // ID FN619726; SV 1; linear; mRNA; EST; INV; 681 BP. XX AC FN619726; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-681 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 80d0dadbbb657221c156a0a3d3489855. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..681 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 681 BP; 220 A; 143 C; 147 G; 171 T; 0 other; ccagcgagca ttcgacacag tctacagcga agttctaagc ttagcgtgat acacagttac 60 aactagttac cagtccccaa gagatcgcca tggcaaccgg cacaataaac ttggaaaacg 120 ggagcataga gcaaacgcgc cagtttttag attcatttga catcgtctca tcagattgcg 180 atggtgtcct ttggtttctt gacaaaccgg tccctggctc ggcgcatacg cttcgtaaac 240 tacaggaact aggcaagcag ctgtatctaa taagcaacaa cagtaccata accatagatg 300 aattacgcaa gcgactggat tcatatggat tagatatcaa accgaaacaa ataataaaca 360 ccgcgaaaac cattagctgg tatctgaaga agattcagtt cacgggcgaa gctttcgtga 420 ttgctacgac acaattccga caaattttaa tcgacgatgg atttaaactc gtaccacagg 480 aaaaaactca gatcatagag cataaacgat acgagggact aaaggctatc gaagatgatc 540 cagctattaa agcagtgatc gtcgatttct gttctctttg tgattggaca agattagcat 600 tagccatttc ttgcttggat agaaaagaag ttctttatat gtgcggttgt agagaggaat 660 ggctcgttta tcatggcaat a 681 // ID FN619727; SV 1; linear; mRNA; EST; INV; 712 BP. XX AC FN619727; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-712 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 2d43889d27ba5ec748328fac7ea96437. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..712 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 712 BP; 244 A; 130 C; 130 G; 208 T; 0 other; tctacattaa gtaaatcaat gttatgttag ctaaaataat agtttcatct gaaattaaaa 60 taaaattttc tcgtgtctct aaaaagaaat gtaaaatgta aaaatgtgtt ccaagtgaag 120 aaagcagttc caggtaaaaa tttccccacc ccgtgtattc gattcgtaac agtccagata 180 atatttcctt atttataatc aataaaataa tatatatgtc atcttcgaaa tcacaaggag 240 tggccagttc cagccagagg ataagccaag ttagaggagg cagagtattg caagtttaaa 300 tttgcacttg gcacctgatc ggctgacgta gtatgaaacc gagggatgca accataggaa 360 ggggtataat tcgctcgatt atttaatagc ttggcctagc gcctggctac aacatcgagc 420 agagccctgt ctcgtggatc ttccgaattg caatctatat taaagccccc atggcgaatt 480 actcattagt agattaacga gcaccaccat gccaatgtta attggagttt cctatagaat 540 tgaagatatt ctggcatatg cgaagcatat agagttcccg gatgaataga ggagatttaa 600 ataaactact acgtagaaac gaaactctca tcaacttcat tcgagattca ttgtttgatt 660 tctgaaaaat atctttagaa cgtctctata gaacatctaa ataaacactt ct 712 // ID FN619728; SV 1; linear; mRNA; EST; INV; 704 BP. XX AC FN619728; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-704 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 3f0af144f7938f4f72ea3d00aa615bea. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..704 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 704 BP; 218 A; 116 C; 139 G; 231 T; 0 other; ttttcgacga tgaaagtggt agactgtagt gacaatgaat tccctcaaaa caatatatgt 60 acacttcaac cagcactgtt atctaagcac tgaacactga aatactagat tgaaaaagta 120 gatcttcaac tcattcgttt cactagatta cttgttgcga tgacaacact gtctagatcg 180 aggttaggtt gagtcaggtg tgcacgagat aaaactgcat attttgcgga gagattatac 240 aaagcaatgc gtggattagg tacagatgac tcgacattga tacgcataat cgtagctcga 300 tcggaaatcg atttaggaga tattaaggat acttatcaaa aaatgtatgg gcaatcgctt 360 gcaggtgata tcgttgatga ctgttcagga gactacaaga gactcttgct tgctctactt 420 ggatgaaagc atttcatgtt caacaattct ttttattttt aaatcttact ttaacgccag 480 gaatatatct tcgcaacatt ccgcgaagct gtatagatgt cttaacttta atttgttcta 540 aattacatca ataattagca gtgattacgg tactggctgg tttttgtttg actttgttga 600 ttatggtgtc gtatatgagt ttgctcgtaa aattagttcg ttcgcatcga aatacactat 660 actaatctct gtgagaccaa tcgagtgaat ataaataata ttgt 704 // ID FN619729; SV 1; linear; mRNA; EST; INV; 662 BP. XX AC FN619729; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-662 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; f80ab3ce361679476c0cd26b4eb14918. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..662 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 662 BP; 204 A; 130 C; 129 G; 199 T; 0 other; tcattccgtc ttgatccgct tgagctgaac gtcgggttgt tcttcgaata caaataacgg 60 gccattcgcg tcctcattac aacattcagg tttaggcagc ttcagttcta cgtttttctt 120 cgatcatacc agttcaacct tgccattgga cgttcgattc aaacagatag aaacagaaat 180 atgtggggcg ttttgttgtt tgcatctctt gcattgacta acggcgtcga acataaaaca 240 atttattcgt ggagcaacgt cgaatataat tttccaaatg acgctattag agattctcta 300 atatacaatg gtggatatat acccgaaaat aatatgccac ttggtctaga aaattggaaa 360 gacaagatat ttattactgt acctcgatgg aagaatggcg tcgcgtccaa tttaaattac 420 atctcaaaga acgatccatc agaatgtcca aaattaactc cttatcctaa ttgggaaacg 480 aatgacatta atacacctga tggcattgta agcatctttc gtgtcaaagt cgacgcttgc 540 gatcgattat ggggtgtcga cacaggtact gatgacattt taggcaatag cacacaagta 600 cgaccagtga gaattttcgt aatcgatttg ataacagata agatattgag aatatatcct 660 ct 662 // ID FN619730; SV 1; linear; mRNA; EST; INV; 649 BP. XX AC FN619730; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-649 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; d6498449b7f05b1717f4ea90e249724c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..649 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 649 BP; 234 A; 121 C; 142 G; 151 T; 1 other; cggtatacgt attaactgtg cttgttatgt gttgctgaga aacgtttcgt ttcgtttcgt 60 ttctcaagga gattatttgc actaataatt gaaaaatgga ggacaaaact gggaatagct 120 cagtggagag tgcggaaaac accagttcag tgaagaagat agcgaagccg aagtctacaa 180 aatcgcaacg gccaaaatct tcgcacccac caacttctga aatggtaaac gctgctatta 240 aggaactaaa ggatcgtaag ggatcatctc ttcaagcaat taagaaatat attgcatcga 300 cgtacaaggt tgatggagag aaattggcac catttattaa aagatatttg aagtcagccg 360 ttacctctgg cgctgttgtt cagacaaaag gtaaaggtgc ttctggatca tttaaactgt 420 ctacaattaa aagctccgag tcaaagaaga tacatcgaac tgtcaaagta tcgaaactga 480 agaaacccaa gaaatccgtg gagaagaaaa cgtcaccgat tcgaaaacct gtgccgccaa 540 agaagtcgaa tcccccaaag aagaccgaag atgctaagaa atcgacagct gtgaaaaaat 600 ctacggtcaa agcagctcag aaatctgaga aagttgaaaa agttnaatc 649 // ID FN619731; SV 1; linear; mRNA; EST; INV; 655 BP. XX AC FN619731; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-655 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0287927fa21c302fffccc0afb0b21e71. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..655 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 655 BP; 216 A; 105 C; 139 G; 195 T; 0 other; gcttctgttc tctcggtata tagttgcgaa gttctgagga aagcgcggca accagactgc 60 gttctttttt ttattgttaa agttgctatt attgcatgct tcgtaacttg tttcaaaggt 120 taattagaag aatgactgtg gaaatcgata aagaagaatt aaaaaaacgt ttgacaccgc 180 tacagtggca cgtcacgcag gaaaagggta cagagaggcc tttttctggt tgctacaata 240 aattctatga gaaaggcacg tacacctgca taatctgtga tcaagaatta ttttcttctg 300 acacaaagta cgatagtggt tgtggttggc ctgcattcaa tgaagtatta gaacagggac 360 gagtcaaatt aaccaaagat acttcacatg atatggtgcg gaccgaggtg acctgttcaa 420 agtgtggtgc acatttgggg catgtattta atgacgggcc aaaacctaca aggaagcgat 480 tttgcatcaa ctcggcatcc ataaactttc attcggcagg agaagagaaa aagatgacat 540 cataaaacta aggagggcat aagtttttaa taaaattttt gaaaatgttt aaaatatcta 600 acttatctta tcgacaaaaa gaatttatat ttcttaataa atatgactct atttg 655 // ID FN619732; SV 1; linear; mRNA; EST; INV; 208 BP. XX AC FN619732; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-208 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; f5193501be18b00fe4404a39ee8d8553. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..208 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 208 BP; 78 A; 17 C; 45 G; 68 T; 0 other; gtatcgcata atcgtatgag agcttttaca ggtatataga caattaattt ttaatgtaag 60 aatattttgt aataattaaa agagaatggc tttgcaaaat gttacgaaga tagcgattgg 120 atggtaggat tatatcttcc gttataggaa tatatgcgtt tacgtatgcg agaagaagta 180 tagaggaaaa aagatatcgt attatgaa 208 // ID FN619733; SV 1; linear; mRNA; EST; INV; 661 BP. XX AC FN619733; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-661 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e903799d5230a032903cf3878b2cd96b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..661 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 661 BP; 202 A; 144 C; 175 G; 140 T; 0 other; agcgtgagag ctgagagagc taggctcgag tacgaaagca ccatgaggaa aggagccaaa 60 caaatggaac ttctcgaaga ggaacgattg agcgctctta aagatctcgc gaacgtttat 120 ttggcccatc tgcaagctct agctccccgt ctgcaacaga gtgcagaccg tttggcagct 180 cctatacgta actgtaacgt gtcccaagac atcgaaatct tgaagaatct tatacgcaga 240 gtagagagca acgacgggtg taacgccgaa caattgctcc cagactttta tgccgaacac 300 gttactctag cgatgaacag agaacgtcgg aaacaagcgc tggtaagagt ccttcatctg 360 atcagacagg acttggagcg cgagaggcgc ggcagggaag gtttggaaac tcttcaccga 420 gccttcattg caacaccggc attcgcggcg gatgagagca cgcaaaacgt aacggaaaaa 480 ctacaccata tgcgttctat gttattgtat ttggaggtag cgaggtataa agttggagga 540 acattggccg aagtagaagg cagtaaacga gacaaacatc cgttggccga acatatttta 600 gtttcaaggg ataaacaggg tttgcagcaa agtgtccctt agattctttc taggcaaaga 660 a 661 // ID FN619734; SV 1; linear; mRNA; EST; INV; 689 BP. XX AC FN619734; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A14 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-689 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; df2c9cad9b265fa9c72dbc03872fa0d7. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..689 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A14" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 689 BP; 237 A; 115 C; 94 G; 243 T; 0 other; ctgcttacct tttatttata gatcccgtgc acaattgcat atacaaggct gtaagaaatg 60 tttgttacaa ctcaattaaa aatgaatatc attaatgtac attaattggg tttgggtttt 120 caacacagct gacaatgcta catgaaacaa taacaattct tctatttcgt aaactaatat 180 caattattca ttcccctatt tttataatta ttttatgatt tatataaaca ttgctagtcg 240 ccccgcggca gtcggattga aaaatttaac ccaaacccac tatattggtg atattttcac 300 tgatgatttt cgagttataa gttataacaa atatttcttg cagccttgca tatacaactg 360 tacacattta taaatactcg tttatgaaca gaagataaat atctgacaac caatactgac 420 gcatacgttt tacgcatacg tacggcacag atttacaact atagactgaa acatactgta 480 gtgatatcaa cagtgtttag agaatatctc gaatgctaag gtcgaccgct ggttatgtgt 540 ataagaaata gtagttcaaa atattgatat ctataacata tttcaaaatt atcagaatca 600 aagatgaggt agctttccta tagtttcatt aaatttgtaa tatcttacaa aatattaatt 660 ttttttggca ttgtatccta atacctttc 689 // ID FN619735; SV 1; linear; mRNA; EST; INV; 667 BP. XX AC FN619735; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-667 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 61ef42e1cf4554951524e54156b4931f. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..667 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 667 BP; 236 A; 120 C; 98 G; 213 T; 0 other; atcgatattt tcgaaagctg aggagcacgg atactcttac cattaaaatc tacttttata 60 ccatataaag cgacaattat tacattccaa agataatcaa caattttttt acaattttat 120 gtaaataata tctctataca tctaaactat tcagttccta cttgttagtt atggtaggtc 180 tacagtgagg ttataaacat aatgatcgtc taagcatatt tatctgaatc ataaacaata 240 cgaaagaaca tgaaaacaat aagttctttc atgataattt ccaccgacca taatacagca 300 aaaattcggc ggactacgtg aaattcaaat aagtacaacg atgaaatctg tgtacaaatt 360 gttttttctg tgccttatct tcatcggatt aataatactg cttaatttaa caaatttctc 420 taacaaaatc tatgatgcat ccaccaaaga agctgtcgtt tctactactg ttaaaaagct 480 tcaaccacca tcaataacag gagaagtaac aatctgtgtg gtagtttgtg gagacagagt 540 ggatgaagcg ctaactatgc tcaaatctgc cctagtcttt acatgtagat ccttacagtt 600 cattattctg gcagaaacca gcttaattcc agcttttaat gagaagttag cagaaatgga 660 actttta 667 // ID FN619736; SV 1; linear; mRNA; EST; INV; 626 BP. XX AC FN619736; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A16 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-626 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; c9c86a9fa1122bd2e30b1f0f75ca7425. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..626 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A16" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 626 BP; 238 A; 89 C; 129 G; 170 T; 0 other; agcagcagca attgatacat ggtacatgta taaaacaaca ctatctgtaa tgattctgag 60 ttgtttggcc gaacaatcag agtaaatatt gcaaaccaca gaagataaag gaaggttcat 120 cgaaacctgt ttgggctgat gatgtatggt tacaagaaca tgcaggtgaa acacttaaaa 180 atgatgataa tacaaaatca agtgatacaa tgcagccaaa aaaagggaaa cagaatcctc 240 aagtatattt tgatattagt atcggaaaac aagaagtagg tagaattatt atgatgttga 300 gagctgatat tgtaccaaag actgctgaaa atttccgtgc cctttgtact catgaaaagg 360 gatatggtta tcaaggaagc acttttcata gaattactcc agaatttatg tgtcaaggag 420 gagattttac aaatcataat ggaacaggag gaaagtcaat ctatggaaat aaatttgatg 480 atgagaattt tgaactaaaa catacaggac caggtacatt gtcaatggca aattctggtc 540 caaatacaaa tggatcacaa tttttcataa gtacagcacg tacagattgg ctagatggaa 600 aacatgtagt ctttggacat gttctt 626 // ID FN619737; SV 1; linear; mRNA; EST; INV; 687 BP. XX AC FN619737; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-687 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; bd0c6bd7ebd4b8b4bf9d54fcad45feb7. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..687 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 687 BP; 182 A; 181 C; 162 G; 162 T; 0 other; actccgtatt ttagtcgacc gggaagtgtg gcacatatca aagtacgaat tttctcgtgt 60 gtctaagtgc cattttagga tatggacgaa atcaatgcaa tgtccttgga ggagtcgcgg 120 cgttcgcagc gtccctacag gtacggaatg gttctccttt gcgttggagc actgataaac 180 tggttaggcc tcgcggagaa ctacgtcgaa ccggtacgat atgtcagtgt tgcttgcata 240 atcgcaggcg ctcttctcat atgtgccgca atgtgttgtt ggctgcacgc accgccaagt 300 agggcaacca tgcacacaca acacacaaat catccaatca cagcacagat cgacgatcct 360 attcacgtaa tctccattga ggaaccatct agtggatcca gacaaaaacc accagattac 420 gaggcggtca cggacgcgcc acctagctac gacgatgcca tcaagttgaa tcctagtcat 480 ctatttaggc cgaacaatag caacacgttg tcattaccaa ccgtacacaa catggttcct 540 agaaccgtgg agattgtttc tagggacccg acgccgtcgc cgccgccgcc ttacgcgagg 600 tgaaatattt ggcaatacca ctcatgatcg cgacaagaaa ggcttgcggc atgctagagt 660 tcctgcgtat ttgcatacat ctaccaa 687 // ID FN619738; SV 1; linear; mRNA; EST; INV; 676 BP. XX AC FN619738; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-676 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; f273ba0735f6429b518dcd8776c47ea7. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..676 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 676 BP; 213 A; 104 C; 122 G; 236 T; 1 other; gtcgatcgtt gagtttggac cgttaaactt cttcttccgg cgcgtgtacc ctttatcgtg 60 ttttcatcta cttattgtta tcgttgctac aacttttaca tctcgagaag gacattcgaa 120 acgttccatt cgatcgggct cctaaataac aataaattat tgtcgtaagt cgggaatgtg 180 attgtttgtc gggaaccggc ataaacgttc tcgaatcttt ctgtcaaccg tcaaaagtgt 240 tagtgagaga aagaaaattg aattcgtgag attcgtgact ggaaaagtgc gtcttagaaa 300 ctagtgcaga aggtcacgtt caacggctgt aggaatgaca acaagaaatt aatcatccgg 360 tgagtcgatt atttcccgcc atttctttaa aaattcaaat gctgtgaaga ataagttccc 420 tcttttttcg tatttaagaa ttctttaaaa attttgaagt tagaatatcg aacattatcg 480 tttgtatacc ataagtagac tacgtatttt tatgcaaatt catattttta atatattcat 540 attttgtata ttgcacgagt tcgctttaga agcagtaagt aataaatatt gaaatttata 600 aattcatatt ttagtagtct gtcatgagtt gtcaaatgan tatagaaatt ataataaaaa 660 ataggttacc aattaa 676 // ID FN619739; SV 1; linear; mRNA; EST; INV; 710 BP. XX AC FN619739; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-710 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 77e964c630ff4dfcda4c9611a9303a89. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..710 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 710 BP; 237 A; 168 C; 106 G; 198 T; 1 other; atttcagctt tggtagaagc ttacgttcta cgcttctata cttgtttgta gcggggaatc 60 cattcaacga catcgggctt ctggtaaaaa tcatactcga ctgttgagat tgttcgcatt 120 ttcgccgtaa aaatgagatt agtgtatata acctgcgttt tattagctac ggcaatttgc 180 tgcatcgcgg ctgataatgc tgctacgacc caaggagata ctaaatctat caatcagcag 240 acagtggatc tagtcaatag tactgcacct attaaaaatt cctcgagtat tgctgcacca 300 gttaaggatt cagttaagga ttctagtacc aatataacta aaaatgccga tgtcaaagac 360 aatattacca gaaccactga tactattcct gctgctacta ctaacatcac tattggtaac 420 aaaacaaaaa ccgatactgt tcctgacaat gatcatggta cagaatctag caattcaaca 480 acaagtacaa taacttcaac acctacaact tcatcaataa caaccaaaca aacacctacg 540 tctgaaaaat ctacacctac gactatagct acacctacta ctacgaacaa tacaacacct 600 atcgtttcat caacaccaat aactactcca gttccatcat cngacaaagt tgttcccatc 660 tatacacaac gacactttga tggactcagc tttttaggtg gcatcatttt 710 // ID FN619740; SV 1; linear; mRNA; EST; INV; 676 BP. XX AC FN619740; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-676 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 91c73ac1bc78d6141897238212029835. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..676 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 676 BP; 215 A; 130 C; 118 G; 213 T; 0 other; cattctgtcc cattctattt tttatataaa aacaaactaa tattcttcct taattgttat 60 caacatcgtt ttacgagcca tctctgtatc atacaaaaaa tgattcgtat aaaagtatgc 120 gtacaattat tttggttctt tttgatcctg cagtcaacaa agtcgaacga agaagattat 180 gaagtggagt tggtacaagt tgtgtttaga catggcgatc gaacaccaac taaatcagaa 240 ttatataaaa atttagccta taatcctatt tacgactcgc ttggctacgg tcaattaacc 300 gatgctggga aaataagaga atttcgtctt ggtgcgatat tgagaaaacg gtatcgtgca 360 tttcttggtg atcatcataa gtatggtagc gtttatgcct acagttcgga tattgatcga 420 acaaaaatgt ctctgcaatt agttctgggc ggtctgtatc caccaacctt gaacgaaatg 480 ggccacttag agttatctcc aatagcaacg cattacgtac cactgattct agacaatcta 540 atgttcccaa tgctttgtcc agcataccta aaggaatata tccagactaa agtttcatct 600 tcggttcgtg caatagtatc gaaaaacaaa gaacttttcc agtatcttgc aaattacacc 660 ggatcagaca tgatct 676 // ID FN619741; SV 1; linear; mRNA; EST; INV; 658 BP. XX AC FN619741; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-658 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 395d581ec312af31db23d87cd9dfaa87. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..658 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 658 BP; 257 A; 98 C; 105 G; 197 T; 1 other; tattgtcata acttcttctt ccaaatatca acttgaacac tattttatga acactattac 60 gcgatatgat atcaatcaat tttacaatgt aacgtatgaa aagaattgat aaatattact 120 gcattttaca ataaactgtc accacagaat caagaattga atatatgtct taaaggacga 180 atacaatcca tatcgtaagg attttgaaat aaaagacaaa aaataaaagc ataatttaca 240 taaaataaag atcatcatga ttggaccaga tttaccagaa ataccactac ctttgaaaaa 300 tatacagcag tatctaaaga tagcatcaca gcatgaccaa cgagatcctg ttgttggtta 360 ttggtgtcgg gtgtatgcct tccaaacagg atttaagtta tcaaccaaaa ctgcaaaaga 420 aacaaatttt ttaatggaat taatggattg gttagaaaaa actaaaaaga aattacacga 480 taatgaagct attacaaatg atgtagctgc ccaggctcat ttagagaatt gggcattgaa 540 gctatttctt tatgcagata aaaatgacag agctgctaat tttgaaaaga acgtagtaaa 600 gagctttttt acagctggat tactttacga tgtattaact gtngttggtg agctaact 658 // ID FN619742; SV 1; linear; mRNA; EST; INV; 661 BP. XX AC FN619742; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-661 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; cc242285e22f3f57cdd6b213c043b257. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..661 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 661 BP; 181 A; 148 C; 177 G; 154 T; 1 other; ctttggggaa gactccagcc agagtggtta aaacacgagc tcactgatac tatagtagta 60 cagttcgaag agtgagcagg agctatccgt ttaaccaaag tgtcgtacag gaacgcgtta 120 tggtggtgtc tagcagctga cagcgcgcgg ccatacacga attcgggtat aagagaggat 180 cgacgaacga gcttcttgcc gattgcttca ccatgatacg aatattcgca gacaccttca 240 ggaatttaat aaccacgtcg gcggtgatcc tgaagacaga ccacgaccat aatcggttat 300 ccagtctttc gggggataat atccttggca ctggagttag gattggtcgg ggcgaggacg 360 cagtcgcggg tatcaacggc aacattatgg attcggcgca atcagccatg atgaagcaaa 420 cagactacgt cgtggatatt gtcttgccaa ccgttctaat tgctctgcta ttcgttggca 480 acgccatcat cgtctacatt atctttcagt atagaaagag gaaagtttca tcgatagagc 540 agggcgagga aatgtctcta cgtaacgcag ccgaggtgcc acaggtgtga ttcgtgttag 600 aatcagcgat naaatttacg gtgccagccc caaaagcgtc agcgagaagg cctacgttgc 660 a 661 // ID FN619743; SV 1; linear; mRNA; EST; INV; 640 BP. XX AC FN619743; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A23 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-640 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; f3e84d2a6cb08b92f3c362635c7be14d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..640 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A23" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 640 BP; 208 A; 93 C; 177 G; 162 T; 0 other; gaagaaaagt tgcttgctgc gcaatgtcta tcagtttcac tttgttcacg atcctcaaga 60 caaagttgga gatagttcca acggttgaaa cgtagagagg gaaaatattc aaagagacaa 120 ccggaaaggg tgaaagtttg agaaacgtgg tcccatggaa cgacgttcca agcattcgca 180 cgagacgagg cgtgccttcc gtatattaat tgttggcaaa tttctgattt ataaaacagg 240 aacgggagaa tgcggccggt atagattcgg gcaagtgcct catttatgcg tttcgtttaa 300 aaataaatat tcgatagtgg aagaggacaa tgataagttg gtgaagatga aaagagagag 360 aaagagagag cgagcgcgcg agagcgaaag agagagagag aaagagagag agagacttat 420 gtaagactta ttcaaagtat tgttgtgcac ttattgccgt gctgtgagta tattggttat 480 tagcaagaga gaatcgtggt gttgaaacac gtgtacggtt attaaaatga aaagtaataa 540 ggaagaaaca cttaccgcgt tgtggcgcct tcgagaaagt atgtattttt ggctgggacg 600 tgttacgaaa tcatttatca ctgttaacgg tgttccgggc 640 // ID FN619744; SV 1; linear; mRNA; EST; INV; 623 BP. XX AC FN619744; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051A24 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-623 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 905a3e5712a80212d7b6b98135cf66b0. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..623 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051A24" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 623 BP; 209 A; 88 C; 117 G; 209 T; 0 other; gactagacat agtatttcat aatattagaa ttggattatt ttatctagat catgatcatg 60 ttactagaaa catagagaag gctaaaagtt taatagagga aggtggtgat tgggatagac 120 gaaatcgctt aaaagtatat caagggacat actgcatagc agtacgtgac tttaaagaag 180 ctgcaacttt ctttttggat acaattagca cgtttactag ttacgaactg atggattata 240 atacatttgt gagatacact gtgtatttaa gcatgataag tttacctaga aatgaactta 300 gagataagat tatcaaaggt tcagaaattt tggaagtact tcatagtaat ccagatgtaa 360 aagattactt attttctcta tataattgcc aatataccga tttctttaaa aatctagcac 420 acgtcgaggg tttgttgcgt agagattatt taattttccc acattatcgg tattatgtta 480 gagagatgcg cattctcgca tacacgcaac ttttagaatc ttatagatct cttacacttc 540 aatacatggc tgaagcattt ggtgttacaa tagaatacat tgatcaggaa ttatcgcgtt 600 ttatcgctgc tggaagatgc att 623 // ID FN619745; SV 1; linear; mRNA; EST; INV; 733 BP. XX AC FN619745; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-733 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 01b0d252fb84b656bbbb986d61781bd6. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..733 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 733 BP; 213 A; 136 C; 160 G; 223 T; 1 other; tcggcagttg attgtacggc taaacaaacg atgaaaaatg gtggaaacta gagtgagctt 60 tccaacaaac tcgggaaaca tcggaaatcg tgcctgtgtc gacaaatgaa caaataattc 120 atcgccttcg gttcgttcgt ttgttaagca acaggtagag taaagaagac tgtttcgaat 180 taacaggagg actatgatgt atcaattctg acggcggcga aataggtccc atgtgttttg 240 ttacgtcctg tacatgcact cgggtttgtt cgcttcgatt atatttgctg aataattata 300 gccgctttca acgtgatggt tatcacgatg gctttcaaga tggttatcgt agaagagcaa 360 ttggagaaac aaaagcaaaa gtgggatgaa acaaggtgtc ggccgaatca cacatacgaa 420 ttatatttat gttctgttcc catatacgag tgacatacat cgttacaatc atgtttattc 480 gctagacaca gtagttttaa ataatgaact taatcgcgaa ggccgttatt gtacatgctt 540 gtacatgttt cgacaagatt tttcatcgat cgacatttat tatttattaa taacagactg 600 gtcgtatcat agaccgttat ctgcgctcgt gtccttatcg ctaaaggcct ctatacacgt 660 tgaaccacgt tgcggcgcat gcatcgacac ttangtctcg tgatttacat gtacgatgca 720 tgtagtttgg tat 733 // ID FN619746; SV 1; linear; mRNA; EST; INV; 678 BP. XX AC FN619746; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-678 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 54f66a7fb3b0c87cfb9b76ed2fa09dfc. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..678 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 678 BP; 228 A; 114 C; 128 G; 207 T; 1 other; aatgggtgat acaggaatgt ctttggcttt gaactcatac aggaaatata taatagccag 60 attacaaaaa aggaggatgt tttaatatta ttcgttcatt ggtatcttat aagatatgga 120 ttcagatgta ttggaatcgg cgattctaaa gcatttgaac caacagaaaa aggatcacag 180 ttacttccag aaggatggaa cacacgtcca aattatgcat tacgttatat aaaggacagc 240 aaattgttca tatttcatgg tataaagtct gatgaagatt tacttataaa tttgttgaaa 300 gtcgatgatc aaaatgtgtc caatgttcaa tttccaatta atcagactgt aaatgaactt 360 catggagctt tagaatctgt aataccttca tatcaaaatg ttataaatgt aattcaggaa 420 gatcttatac acgcattgat tcctggcaat atggtcgtaa attctactca aacaagctct 480 acaaatgatc gatcaagaga caatccatta agggtaggag agccgacatg tccaccgtct 540 gcatcatatc catggaaccg tgatgctgat cctttaagag taggcgctgc agatttaaat 600 ccatttgccc agggtagtgg tatgattttt gatccatttt catcancacg taatcgtatg 660 aacccatcta gacctggt 678 // ID FN619747; SV 1; linear; mRNA; EST; INV; 657 BP. XX AC FN619747; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B03 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-657 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 48af983671140860360a00d31cee51b6. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..657 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B03" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 657 BP; 273 A; 88 C; 125 G; 171 T; 0 other; accgtgtgct cgatcgactc cgtttataag taaaaatgat aaagaaaaac aaaaagcaga 60 ggatgtatta aaatcaaaat gcccaaatgt tgcgaaggag caagtgtctg taggacaaac 120 ggtagcaagt tctgttggtc aaccggagca gtatatcaaa acttcgagtg agatgaaaag 180 tcatgtgcat cccagtattc cgggtaatca gaaagagcaa aataatattg aaataggaga 240 ttcaaaatca ggtaaattac atccgaagaa gaacttttac gaatggaacg gaaacgaaaa 300 cagatggaga aacaagcaga gtataagaga ttaatgaagg aaagagaaca acagaagact 360 aatcaaaata aaaaaagcaa tacaagatac tagtcaataa agttaaaaac tttatatgaa 420 cgagtcgtat aatcacagtt gtatacacag atgtggaaac tcctgtgtta aaggtagtta 480 ttcctgattt gaagacagaa gatttcttga attgtttaac acacgcatga tattgaatag 540 tcaaaacaat ttcaacaagg taatacatgg tggaagtaaa caaattaatt cttttgtaat 600 ttgaataata ctttgtaaat aggtatacat tatttctttc ataacaattc tatttat 657 // ID FN619748; SV 1; linear; mRNA; EST; INV; 673 BP. XX AC FN619748; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-673 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e4a7ff09c675126236498ff7daf7fb05. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..673 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 673 BP; 264 A; 78 C; 116 G; 215 T; 0 other; gattgtgagt ggatcaggaa gtaactatga attttaataa attacgacaa tgctataaat 60 ttatttcttt caaatcatta catcaaagtt catatgttat accaaaacca tttatttatg 120 ataaattctt gaaaattgct tcatatagca tattttttca tggtaatcta caaaaaacat 180 tgaaaaggag cctttttcaa cctcaatact tcacaataca gatgttaaaa ccatgtctgg 240 aggtgataaa atttgcctta acgttgatta taatgagatt ctaaaagctc aaaatgatag 300 tagtgtttta atcatcgatg tcagggaaga ggaagaaatt gatgaaactg gaaagctacc 360 tggaagtatt catataccaa tgggagatgt tgcaaataca ttgttgagct tttctgagaa 420 agattttaaa gaaagattta agaaagaaaa acctaccaaa aatacgaaga tcatattaag 480 ttgtagatct gggaaaagaa gtggcatggt tcaagaagaa attcaaaagc ttggatatga 540 aaatgcatac aattatgttg gaggatggct agattgggaa agcaaacaga aagtataagc 600 tattgttata attattttta catgagtaga tatatttatt tataaatatt agtaaacacc 660 tatatataat ttc 673 // ID FN619749; SV 1; linear; mRNA; EST; INV; 687 BP. XX AC FN619749; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-687 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 6a89d39c86ba6ba3ea5be4c3b4266db6. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..687 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 687 BP; 207 A; 157 C; 187 G; 135 T; 1 other; catcgggacc gtaccatgag cggaggctcc actccaagca tcatcggaga atctctccca 60 agagtcaaca gcgtcaccag cgtgctgaaa cgattattca gtaaagacga tagaccagat 120 ggcggtacat caagtgggac taagacacct ggaaggcttg catcttctag ttcggccgct 180 tcgctacaaa atagagccgt tgcaggagga ggctcgatga ggatcggagt aatcaaggaa 240 gaagcggacg aacagatgac gttaacgtcg atgaagtcgg agaagagacc ccacgtgcag 300 agtatatttt cgccgggacc accacctcca agtgctcccg tcgctgttcc tggcatggaa 360 cactcacgaa atggagagat attttcgtgt agcgctcctg taactggtgg tgtgttaagg 420 ggaagtaacg gtgagattgt caacaacagg agatacgggt ctgattcaag aacacaacgt 480 acttcacagt cagcacgatc gggcattgct tacgaaccag cagccagcta cgtggacagt 540 gttgcggatg atcttataag cagcgatagt tcatccgcta gcgttaactc cgatgacgaa 600 tttacaaaat tgaagacnga aagagaaaag caaagacgtg ataaagtaga acgaagattg 660 gctaggagca ccagcggccg caataac 687 // ID FN619750; SV 1; linear; mRNA; EST; INV; 748 BP. XX AC FN619750; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-748 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e1e2930f5f14b0afc41de94929740608. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..748 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 748 BP; 249 A; 110 C; 132 G; 255 T; 2 other; tactaacaat atactagatt ccagtaatag ttaattttta aaatcgttat tgataaagtt 60 caaaagaata ctgaagcaaa agtatatgca tttgtatgca ngcgtttttc ataggatgtg 120 atatatccga aaaataagat agttctaaat caaaaaaatg aagcaattct tagtgttatc 180 acatggtcat tagtcactaa gagacagtta tgttgaatgc atgcctaata aacttaatta 240 ccggctgcaa atcgtatcgc attgatttct gctaaagtca ttacgtattt caaatcagat 300 tcgaggtcca aagaagtggt tggattataa ctttaccaaa tattagcaca agtgtttaag 360 tgtttaagtt tattcaggat gcatctgctt ttctccatta ataacaagaa atttaatcaa 420 caaagaaatg atcacgtaaa agcatcaaga attgttttct tcagttaaaa cgtctttggt 480 ttgacgtatg agtttgataa tgcatcttat gggatactct gtacatactg cagtatcatc 540 taattctgca ttgcactagg gtataaggtg gtttgctttt ttcattggaa ataaatgttt 600 ggtgagcatc gttcaattca agaattgtta tgtgtgcacg agtgctcnta aacagagtac 660 atacatcgcg ttaacgtgca cactttttga aaaatagagt agccatattg ttttgcagca 720 gtgtttcacc tatggacaga acttttta 748 // ID FN619751; SV 1; linear; mRNA; EST; INV; 670 BP. XX AC FN619751; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-670 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; f931d24b4a688a8688cb2156608bad16. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 670 BP; 260 A; 81 C; 119 G; 210 T; 0 other; tacgtatact gaacatgtgt aatataaata gtaaatacta gtgttgatat aaaatcgata 60 atttacgtta aatcattcgt gaggaaagaa gatctattaa catattcaac atttgagtaa 120 tatccagaac tattattata attaaataca tgatcgagca catgatcaag aagattgata 180 gatttatgaa caccttcaaa cttaacctat tttacgataa gagattatta taagactttt 240 taaacaagcg taatgaatga tgcgactaga acgggacaga aaaatttata tgtattaata 300 tcgtcatcat tcttggttat cgcggaatcg gtaaaatatt aaatttaggt taacatcagg 360 ttttagtggt gaaaccgtat atatcaataa ttttattgta caaaagagta taatattaat 420 tgaatgcgat taggaactta tagtaacatt ataggaaaca atgggtagtt attttgatcc 480 ccttggtttt actaaacaaa cagcaacttt gttgcgtggt ttgggtgcat ctgagttaat 540 agtagaatgc aaaaagagaa atatatcaac ggatgtatta caagagctta ctgtacaaga 600 gctaataatg ttaggcattg accctaaaaa ggccgaagaa gtaaaaaatt gtttaaacgc 660 caggaaagga 670 // ID FN619752; SV 1; linear; mRNA; EST; INV; 676 BP. XX AC FN619752; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-676 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8155a189adf56030dab9dc31f2bb1a41. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..676 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 676 BP; 245 A; 103 C; 132 G; 195 T; 1 other; taacctgctg tgtatacaaa caaaatctgc tggtcggaag gaaaaacaga aggtgtaaag 60 taaaagacaa agacataaca gaagtatatt ttattgaata actattcatt tataattaac 120 gtattttgag acagccaagg ataagctata aattgtatat aattgaacat gcagtgatac 180 aagtagaaga aaacaaagag acgtgtgcgt ttttctgtga tatatgaaat cttactagaa 240 aattataaaa tattgagatc cagttttaat catttctaca ttttatcaaa tgactgtgga 300 tggtactaac agtaatgagc aagttattct tgtaacgggt ggttatgatc atactatcaa 360 aatatggcaa cctcacacag gagtttgcca acgaactgca gaacacacag attcgcaagt 420 caatgcttta gacataactc cagataaata tctaatagct gctgccggat atcaacatat 480 aaggatgtat gacttggttt cgaacaatcc aaatccaatt attaattatg aaggagtgtc 540 aaagaatatt acaggtttag gtttccagga agaagggaaa tggatgtata ctggtggtga 600 agactgttca gcacggattt gggatctcan gtcaagtagc tttcaatgcc cacgcatatt 660 tcaagtgtca gctcct 676 // ID FN619753; SV 1; linear; mRNA; EST; INV; 670 BP. XX AC FN619753; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-670 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8143ac0e444a797cb9d0c223e39c604b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 670 BP; 201 A; 149 C; 161 G; 159 T; 0 other; gttcacttct atgattcgga cagttggttc gttgtgacca tctcgtacca tcctcttaca 60 aaacaactgt agccatggcg gataaagaga agaagaagaa gacgaaaaag aaggaagagg 120 cagcgcctgc tcctgcacca gaaccagaac cagcacctgc accagcagaa gaaaagccac 180 caactcctac cggtacccct aaagaatctg gttcgactag agcaagtagt cggggcagcc 240 gcaaagctaa acgcactgga tccagcgtgt tttctatgtt tactcagaaa caagttgccg 300 aatttaaaga ggcattccag cttatggatc atgataagga tggtgtaatt gggaagaacg 360 accttcgtgc cacttttgac aatgtcggtc gtttggttac cgataaagaa cttgatgata 420 tgctaggtga agcccctggt cctatcaact ttacccagtt actcaacttg ttcgcaactc 480 gcatgtcggg atcaggttcc gacgacgatg aaaccgtaat tgctgcgttc agtaccttcg 540 acgtaaacgg aaagattgat ggtgaaagat tgaggcacgc tttgatgaca tatggtgaca 600 aattcaccgc gaaggaggcc catgatgctt atgataatat gtacattgat gacaagggat 660 ttatcgatac 670 // ID FN619754; SV 1; linear; mRNA; EST; INV; 713 BP. XX AC FN619754; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-713 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; c831db4c5852faf40c9774fbe3ad99fa. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..713 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 713 BP; 205 A; 130 C; 175 G; 202 T; 1 other; tcatctggga cgtcataagg ctggtatcga ggagactgtc aaactggtga aatccgcagg 60 tggtatttgc tatggttatg tttgcgacct atgcgatcgg gaggatattt acaagaaagc 120 agagcaagtg aagaaagaag ttggaaaggt aaccattctg ataaacaatg caggtatagg 180 aaatggctat aaacttttgg acacgccgga caaccttatt atccgaacaa tggaagttaa 240 cgtaatgagt cacttttgga caacaaaggc gttctcgcct tcgatgatag aagataacag 300 aggtcatatt gtgagcattg caagtatggc tggacatata ggagtgtcac atttggtgag 360 ttattgcacc tcaagattcg cggcgattgg atttgatgaa gctcttcata tggagctagt 420 tgctgaagga tataagatta atagcacggt ggtatgtcca tttttcattc gaagcaccgg 480 tatgtttgat aacgtttcaa caagatactt gccgaggctc actccgaacg tcgtggcgga 540 tcgaatcgtc tccgctataa gatgcaacga anaagttgct atagcacctg gttatctttg 600 catttttctc attctgaaat gggcatttcc gtgggaatgt gcgaatatgc tggtacgagg 660 tttacttttt aataagattc accatcagac tcaccatctc ctcctgtcaa gaa 713 // ID FN619755; SV 1; linear; mRNA; EST; INV; 194 BP. XX AC FN619755; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-194 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 18fee22133811f028bcc85625bdae85f. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..194 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 194 BP; 53 A; 23 C; 28 G; 89 T; 1 other; gaacacaaat ttgttcaatt tctttcctcg gtttgcttta atctatgttg aatgtcagtt 60 atattgtaat gtttgatatt tgttgaaatg aacttttgga ttatttctaa tcgcttatta 120 atgttttaac tcttatgaaa taaccgcaaa gaattttaat tgcacgtgca ttgtttnaat 180 gcgttttatt tatt 194 // ID FN619756; SV 1; linear; mRNA; EST; INV; 250 BP. XX AC FN619756; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-250 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 1975e4189a3a736eab1b482ecf078bfb. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..250 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 250 BP; 69 A; 35 C; 39 G; 106 T; 1 other; atttgttcaa tttctttcct cggtttgctt taatctatgt tgaatgtcag ttatattgta 60 atgtttgata tttgttgaaa tgaacttttg gattatttct aatcgcttat taatgtttta 120 actcttatga aataaccgca aagaatttta attgcacgtg cattgtttaa atgcgtttta 180 tttattangc gaagcaaacc tcgctccctt tatacgatca gtgaaggtca ctattttata 240 aaatggctga 250 // ID FN619757; SV 1; linear; mRNA; EST; INV; 679 BP. XX AC FN619757; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-679 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0d80d1c8c9c1e51d3c15f5f26620e360. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..679 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 679 BP; 249 A; 117 C; 117 G; 196 T; 0 other; ttctatgtac gaatgtgtga acgaatgtat acaataacac tacagaagga acgaatgcta 60 taaaaccaga atctctattt cttattgcca aaaatacgat aaaattgtga aattgccacg 120 tatagtttgt tacaggtaac tctaggataa aattgctatt gcagcttgta tattgcagct 180 aacagctata atcctaatta taactgaaat tacgttatcc taatacagaa ttatgttgtc 240 accttcgtcg agtgcaaacg ataacgagga tgtctttact tatcaaatag aagttttatt 300 tccaaatatc acagaagaca aaataagaac cgttgaattc ttagatgcag ctcgaggtct 360 tgtgcgaatt attgaaaaac ttggcaaagt gtttgctcca gttaaatatg gtatccaggg 420 caacattgat aaactcacgt cccggcatgc aaaggataaa gaaaaaaacg cgattctaca 480 aaatatgatt ctaattgaaa aaaatactga aactaaatta atcgctacag atgctctaac 540 gcggttaacc agagctttgc atatgattct gttatttttc gaacaaattg tcgaggactc 600 gaaaacggca acaccaacag aggatttagt agcattttta aaaaagctta taaagaagct 660 ctacagccat atcacggtt 679 // ID FN619758; SV 1; linear; mRNA; EST; INV; 704 BP. XX AC FN619758; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B14 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-704 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 23beab342e2d376b63883a278cb55b39. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..704 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B14" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 704 BP; 203 A; 111 C; 108 G; 282 T; 0 other; tttcatcggt gagtatgtct tctgctgaat ttccggttgg ctgtcattcc aaaattttac 60 caactacctg aaaaatatga cttgccaata ccaatttaac tattcacatt tgtatcgttt 120 tacgcttttt actgtttatc gttaacatta acattgatcg gtagacttct gtcaggatat 180 ttgaagaaat tgaattttcg aagattcacg attaaagatc tttctttgat tgaaaaaccg 240 ttttccgtga aatgtaagcg gctgaaaata atagcgagat agtctgcgaa ggaagttctc 300 gaagtatttt ttataaatta aaatctaacc tttctagtat atagtttcag gcggtatggt 360 cgttacctaa cctactttat tacttcttcc atatttaacg tttaattgtt tatgtatgta 420 gatgttcatc gtttaatgct caactgataa tacttaactg atgcgtatat aatcgataga 480 ttattgattc ccgtgcttaa gatatcgttt atagcttctt tagagatata tacctattta 540 ttttcttatt aatattctac tttccattca tatatttgct cgtgatgcga tattacgttg 600 tctgttaaca ttgtcattta cgttgaaata aagttataca gatttagacg tattaatttc 660 atcgcgatat atcgtcatgt atgtatacgt atacatatgc tttc 704 // ID FN619759; SV 1; linear; mRNA; EST; INV; 620 BP. XX AC FN619759; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-620 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; c99b5c004279704bd8d3ebf93dd3267c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..620 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 620 BP; 202 A; 128 C; 127 G; 163 T; 0 other; ggcagttgcg cgcaaaagtc tgtttattta cgtgctgtcg atgattaatt gatcgcgaaa 60 tcgcgtctac aagcgatatc cgatgagtga gttcaatgga aacgaaaacg agatggtgta 120 gaggtgaaaa cataaccaaa catattcgtt gaatataaaa gaaagaaaac gctagtgaat 180 gtcgtgactg tggaattcag cctatcatca tcgttctaac ctacttcacg aaacatcgta 240 ttcttcattc tggaattttc tgacgcgctg tcaacgtttg tttgccaaca gtggttcggc 300 atgatgccat gttcccgcaa acgctgagca cagaaccgtg caaaattgtt accatggctg 360 acgaagtact gccggcgagt ttagaaaagc tcgaaattga tcgtctctca tcgagctgtc 420 ctcatattac acctactgac aaagtgaagt cgtcaaatcc gtttagaaca acaagtaaaa 480 tcatcagttc tgattgcagt catttatttc gaagacgatt gatacctgtc aagccaacgt 540 taaccacaaa cagtcccaga ggcgttaaaa gcacaacgaa ttttaaacca tcaaaaaatg 600 aaagagtact ttgataaaat 620 // ID FN619760; SV 1; linear; mRNA; EST; INV; 680 BP. XX AC FN619760; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B16 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-680 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 34aa8fdc7fb53cbbc42359f34f43e2db. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..680 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B16" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 680 BP; 224 A; 123 C; 133 G; 200 T; 0 other; aattattctc tagtaattaa ccgagcaaaa cagccacgta agtaattgta tatgtggaat 60 taatgtttaa aactaacgta agtgaaagaa acagccataa gtttgaggtc atgtattatc 120 gtggttttat ttccttaaaa gtgataaagc cattgcacgt tgcaaacttt ttctccgtaa 180 actttacttc tattcggcct cttaactttg tgccactaaa ttgtatctga ctgtaattcg 240 taagcaggcg aatctatcac gagaatgtag ccgttttgac ctcaaactta tgcacacaca 300 tagagagaaa gagagaaaag atgaagaaac gcgggcttgt acaatacgcg ccagagtata 360 ttttatattc gcacgcgctg gtgcgcgcca atattttttg tatcatatta aaaaaaaaaa 420 tcgaaagatc gatcggtgga tgattcgtat ctttggaaca gcgcaaaacg aacgagagat 480 tattaccata ctgcacaaaa acaattgcaa cccacgttgc tccagagtca ataatgggtg 540 ctgtttaagc gtgacttgtc gctattttaa tgagtgtgta gtatgtcgag tattaagacg 600 tttcgatgtc ttaaaagaga acgaaagccc attaagcctt atatttcgca aaacgcgatt 660 tgtaacaagc atttgtaccc 680 // ID FN619761; SV 1; linear; mRNA; EST; INV; 664 BP. XX AC FN619761; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-664 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e35f9b1fa373013394922fdf71d50af9. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..664 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 664 BP; 237 A; 82 C; 104 G; 241 T; 0 other; agttcctcga tttccggtca gcacgattca gcaatacgac gggacttgta tttgtataaa 60 aaaaaaagta ttatgtcgtt tcatggttat ttaaaccacc gattaagatt tcacacgagg 120 aaatgaattt tagaaagaat tattcatatt tttgtttaga acattttccc aattatataa 180 atacttgatc aatattttag caaatgacag ctctaattag gagaagtaat tttcgtctaa 240 aaacttgttt ctatctctag tagtttgcga gagataatat aaaatagaaa aatagttgaa 300 tattcggggg tctattttac tcgctcggag tgaatttggg cagcaagaaa aatattttat 360 tacgtttacc tatatatctc taaagtttca taatttcctg aatttccggg ttgggaatat 420 tcccttgtca agttgcaagg tactcgtaaa tttaactaat tgtattttgt aagtttttat 480 ttcagttttt tgtagtataa tacaaaaaca tttggaaatt ataaaataca gttcttgtat 540 tttaaagtat tataaatgta gaacaacttc taaattttct ttttgtgtaa taatttcaaa 600 ctaaattagt aagcagaata atccgagaga gagagagaaa gaaaacgcaa aaaattttta 660 ataa 664 // ID FN619762; SV 1; linear; mRNA; EST; INV; 721 BP. XX AC FN619762; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-721 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b7f9fe3012f3fdee396307bcab206d55. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..721 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 721 BP; 222 A; 130 C; 151 G; 217 T; 1 other; aggttcacat tgacaaaatg gtttcgaatt gcttgaattc aaatcgtttg aagaagcaac 60 atgactaatc tgaatagttt aattgtcatc tttatccaag actcgcatag tcaatcgagc 120 cattcggaaa tcacattgac cgactccaaa attgaaaagt agagaaaagt aattgcaagt 180 atcgcaagtg gtgggacaat atgcgataaa agtgaaaaga tgagcgtggc gttaagctct 240 aaggaactag aggttttgtg agaatcaatc gtgtacttgg acgatgaaat tgcgaaatta 300 tgagattgtc caaagatgaa gttggtcaat ttgactttcg ccgttctctt gatccgttct 360 cttagaacgt tcatagagct ctgggtcttt tcgttctata ggattaatat aaatctagaa 420 attgtacagc ccaactttaa gtctatcgca gtttcgttcg aacgacgcta cttcgtcctg 480 tacaaatttc gattcctccg atgaaacgtg tattcacagc aaagtacttt tttctgtaat 540 cttattatgg cttgcgaaag tgttcgaata atcttgatcg ttccgaggtg acgcgtatca 600 cacgaacaat agaaaactcg gaaaataccc gaaggtcgga tgatacccgg cgtcgtacgg 660 taaaatcgta aaattgattt cgataactta catcgtacgt gcngttcagt ttagttgact 720 a 721 // ID FN619763; SV 1; linear; mRNA; EST; INV; 677 BP. XX AC FN619763; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-677 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 3f516676cead84896eff76614d93c891. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..677 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 677 BP; 196 A; 105 C; 116 G; 259 T; 1 other; tagtaattta cgcagatgca ctacgttatt gtattcattg caatttccgt atatgcaagt 60 gtttgtagaa attgaaattt ttttatttag tgaacaagcg tttaattaca aaaatggcag 120 atactgcaag tgcaacacca gaagatactt cagtacacat ggagaaaggc tggtttgctt 180 tgaagcaaca catcattgat aataagatta aagttggatt atgggttaca agacttttca 240 ctcttatatt tactattggt tatatcattc caatatttgg taattcttat aatatttatt 300 ataaagtatt aatgagtaat gcagcaacta gtgcattacg cctgcaccaa agagtgccac 360 atgttcaatt gaatcgacaa tttttggaac atttatttat tgaagattcc tgccattatt 420 tattttattc tttaatattc ctctatactg ctcctgttac attggtgtta actcctatat 480 ttttatttgc attgatgcat tttgctagtg gttctcttat attgcttgat gacaaaatag 540 ttggtgggga gcacgcctgt taatatctct ggtggagttt caatcacgaa atattttacg 600 actttgtgca ttatctgaga taattatctt acctctcaca gctcttcttg tattnacggg 660 acgtgctggt ttattga 677 // ID FN619764; SV 1; linear; mRNA; EST; INV; 652 BP. XX AC FN619764; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-652 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 5d5b6ebaa2832094e9644773f64fe109. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..652 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 652 BP; 213 A; 120 C; 171 G; 148 T; 0 other; tcttcaactc gaccgaacca aagcgaaagt tacgcttctc atattcatat tgatgtttgt 60 atggtgaaag tttgcatcgt gtcatgtcat cgaacaacgt gcgctacgat tcttcatcgt 120 tgttgaagaa aaagattgaa acggagaata gagaagagga ggaacaaaca gaggagaagg 180 accaatgtcg aaaaccgtgg agaactttgg acgccccttt ggtcgatcgc gacgataact 240 tatacgtagc tgttcgtcag agcttgcaca gagttaatcg ggcttcacaa aattcaatgt 300 ctccgctaag aatcgctgaa actgggaata gaagtggagt gtcgcaggag tcgagcatgg 360 gaagattttc caccataaag gcgttctttg atatgggcca ggaggaggga acaaggtaca 420 catgtaggac caccataatg ggcaagctga agaggacgct ggagttgaga aaacgaggaa 480 cgggttccat ggaatcgacg atgaacggtt tgaagaaaga tgtaaacggc aatataatat 540 cagactagaa gcgaactacg aagggacttg aaagctactg tcgttctacg atcagcggaa 600 taaaatcatc gaagaggaaa caaactgttt cagaatctac cgctgtttta tc 652 // ID FN619765; SV 1; linear; mRNA; EST; INV; 636 BP. XX AC FN619765; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-636 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 27bea210086f3431ebc031d31964e362. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..636 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 636 BP; 194 A; 102 C; 156 G; 183 T; 1 other; ggattatggc catgaggaag tttccttgaa gtttggccga gatccccgtc aatttagtac 60 attgagtaaa gagtttttag acgatggaaa tggtcacgta agtggtatta aaacagtgtc 120 agtatcatgg acaatggaaa acggacgttg gaaaatggga gagcttcctg ggacagaaaa 180 aatatacaaa tgtgacctag ttttactcgc tatgggtttc ttaggtccag aaaaatatat 240 tgcaacagaa ttaaatacgg aaatggatga aagaggtaat ttcaaaacac cagttggaaa 300 ttacaagaca agtttagatg gaatatatgc agcaggagat tgccggcgtg gacaatccct 360 ggtagtttgg gcaatcacag aaggtaggca agctgcaaga gaaatagatc tggccttaat 420 gggggaaact ggtcttcctg tatccggtgg tgttataact ggcgttatgg cttaagatgc 480 atacgttcta agtgtagtcg atcagaattc ggctatttct ttctaccctt ttcgttccta 540 ccgcgttttt cttctctttt ttaaactttt tcagtgatta aaccgtgcaa agtatgcgat 600 cagtgtaccg gtaggagaac naagtacatg taaatg 636 // ID FN619766; SV 1; linear; mRNA; EST; INV; 626 BP. XX AC FN619766; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-626 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; da7c3839328b5f1ccd08cecc97cb8f68. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..626 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 626 BP; 182 A; 135 C; 139 G; 170 T; 0 other; gggggaaaag gaggggacga taaggacaag actcgactac agtgtgccgg tttctaagat 60 tgttgcgaac ttcattaatt gcaagaaaac ctctcgttga aattggaact cgtcttttat 120 cttccatggc tcaagcgttg caaactcacg gtgtcgtacc cgacgttata gacaaggtcc 180 cacaaaatgt actacaggtc acttatccaa ataaccttgc cattgaaatt ggcaaagtat 240 tgactccgac acaggtgaaa gatccacctt ctgttaagtg ggatggcgat gcaagcgttt 300 tctataccct ttgtatgact gatccagatg caccaagccg caaagaacca aaatttcgtg 360 aatggcacca ctggttggtg ggaaatattc ctggttctga tgtttccaaa ggtgaagtac 420 tctctcagta cattggttca ggtccaccag aaggcactgg acttcatcgc tatgtgttcc 480 ttttgtataa gcagcctcaa aagcttacat tcgatgaacc tcgtttgact aatcgcagtg 540 gagataatcg agcaaatttt tctattaaaa aatttgctgc aaaatacaaa ctgggagacc 600 ctattgctgg gaacatgtac caggct 626 // ID FN619767; SV 1; linear; mRNA; EST; INV; 696 BP. XX AC FN619767; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B23 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-696 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 67886f24e159068c061920ee0412e337. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..696 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B23" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 696 BP; 200 A; 109 C; 118 G; 268 T; 1 other; aggtagtaat ttacgcagat gcactacgtt attgtattca ttgcaatttc cgtatatgca 60 agtgtttgta gaaattgaaa tttttttatt tagtgaacaa gcgtttaatt acaaaaatgg 120 cagatactgc aagtgcaaca ccagaagata cttcagtaca catggagaaa ggctggtttg 180 ctttgaagca acacatcatt gataataaga ttaaagttgg attatgggtt acaagacttt 240 tcactcttat atttactatt ggttatatca ttccaatatt tggtaattct tataatattt 300 attataaagt attaatgagt aatgcagcaa ctagtgcatt acgcctgcac caaagagtgc 360 cacatgttca attgaatcga caatttttgg aacatttatt tattgaagat tcctgccatt 420 atttatttta ttctttaata ttcctctata ctgctcctgt tacattggtg ttaactccta 480 tatttttatt tgcattgatg cattttgcta gtggttctct tatattgctt gatgacaaaa 540 tagttggtgg ggagcacgcc tgttaatatc tctggtggag tttncatcac gaaatatttt 600 acgactttgt gcattatctg agataattat cttacctctc acagctcttc tttgtattac 660 gggacgtgct ggtttattga caccatttat ctattt 696 // ID FN619768; SV 1; linear; mRNA; EST; INV; 633 BP. XX AC FN619768; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051B24 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-633 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0fbc6d32026627d17544151e5f4c95cb. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..633 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051B24" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 633 BP; 224 A; 75 C; 108 G; 226 T; 0 other; atgtcgatgg cccgccccgt actatataat ttcatatcac tattaatgtt gtaacttgta 60 acagattttt ctgtgattgt aattttattt tatgctcaat accgatttat attgcaagtg 120 acacaaagtg cttatctaca tgaaatatac gatacaaatt ttcattgaat gatagatctg 180 tattatatat ggtttgtgac ataacctaac gatcatttac gtcatttgga ttgttgtatt 240 agagtaataa gaataagtat ataaagagtg ctgctgcttg cggtcaataa taagaggagc 300 ttgtgaaatt aataattttt ttcgaaaaat agttatcgaa gtcaagataa tgacttttct 360 atataacttt tttaaaagat cttctacatt cacattgggc attctagcag ccaccttttt 420 cttcgaacga ggatttgata tgattgggga taaaatattc gacaggctga atgaggggaa 480 actgtggaaa catattaaac atcaatatga agaaaagtaa tcaaaagtgt taattatgtt 540 atgttagatg tgtatgtttg tgtatatcat atagatatgc tattgtataa ataaagaaca 600 attcgaataa aaatatgata taaatttaaa aag 633 // ID FN619769; SV 1; linear; mRNA; EST; INV; 710 BP. XX AC FN619769; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-710 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 673b7b2db6d1d4efa8634b6e0484a0f8. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..710 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 710 BP; 266 A; 98 C; 162 G; 183 T; 1 other; attcagatgg aggacggtga aataaagcgg acgttgggct ggataaagag aaaaagtcgt 60 acgcaggaat accggaggcc gaatttgtag acgatgtcga cgcttttatg gcaagacctg 120 aaaatgaatc tgcagacaaa gtactgcaga tgttggatga gagccatggg aaatataaat 180 ttatggaata taatttattc aataaaagga gacgtctgaa agctcagatt ccagatctag 240 agcgatcgtt agaaatgatt aaaaaattgc agcaagagaa gaacaagagc aaagaattag 300 agacacaatt tttactatcg gaacaagttt atgcaaaggc tgttattcct cctactgata 360 aagtgtgctt gtggttagga gctaatgtta tgttagaata tactttagac gacgctcaag 420 aaatgttaac aaaaaatatt gaagcagcga agagaaatct aggttatgtt gaacatgatt 480 tagattttgt aagagatcaa tttactgcaa tagaagttaa tatggcacgc atttataatt 540 gggaagtaaa acgcaggcaa gctgctaaac caacatgaag gctgtctttt tttgataaat 600 tcaatttgca cacaacaaat gcctaaaact cgaagggtta tttacattgt caaaagtaaa 660 gaacctggga ataaaaagta ccgttggaaa ctgtncatgg aataaagggc 710 // ID FN619770; SV 1; linear; mRNA; EST; INV; 685 BP. XX AC FN619770; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-685 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 1e0404c6cc607e868f7808d786f00f0d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..685 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 685 BP; 231 A; 107 C; 133 G; 214 T; 0 other; cataaccttg caagtccttt aacaaaagaa atgttgtctc taattctaac ggaatagttt 60 gctcttccag aaatatgaca ggaccgtaat attcgtaagt gaaaatagct catatatcac 120 cattcaacat ggtagatctc aatttaatta gagaatatct tagtatacaa attaatgaaa 180 taatttgttg gtggtggttt tacatcaatg aaatttcatg gcagttgcta atagctgtta 240 ctgcgtatct ttgcgtttgt atttctcttt attcaatctt gaagagagtt aaccacacag 300 cgtggcagct tccgggtcca cctgccagaa tactgttagt tacagctcat cctgatgatg 360 aggtcatgtt ttttggtcca ctggtgtact gggttacaag atcaaaggat agtaagattt 420 acctcctctg tcttagcaat ggtggagata agaagaggaa agaagaatta tggaaatgtg 480 caaaactatt aggaattcca gaagataatg taactatagt gatgaataca gagttacctg 540 atgatcgaaa tgtacaatgg ccaacagaga tagtggcaga atgtatcttg caacatatag 600 agatttataa aatcaatgca gttgtaacat ttgataaaca tgggataaag caacataaga 660 atcatatttc tttatactat gccat 685 // ID FN619771; SV 1; linear; mRNA; EST; INV; 672 BP. XX AC FN619771; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C03 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-672 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 2ac8e1bb83bbf2bcdf2d9cc136222af5. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..672 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C03" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 672 BP; 236 A; 93 C; 100 G; 243 T; 0 other; agtttgctgt tatacgatat agtacatata tttctactgt ttctactatt ttttctaaaa 60 ttctatacac tcaattccat actacgatag aaatgattca ttattttcta ttgagttgat 120 agtatggtcg tataattgaa tcctacatgt attcctacat gtattcctac gtgtagtcct 180 acatgtattc tagtcataac ctgtaaagta acaaataaaa aatgtgacaa aactaaatgt 240 atataatatt aaaatactgt aagttgatat gttatatgta attaataatt taagaaatga 300 attactatat ttgaacctcg atattcatga atataatcgt ggaaaaaaaa tataatcgag 360 ctgctataac ctagttcaat ctgttattga ggcattatga cgcttagtcc gaaagattta 420 tcaaaattat tggatatcct tgaagaagaa aatctggata cgagtttgga aaatctttgt 480 aatcagttac atcaaatatt tccgaaagaa gatcgattta aagtgggaat gacattggtt 540 ctccttttac agcatataga tttattgcca aaaaatgttc aacgtataat agcagttgca 600 ttgttatttg atctttatag aggagaaaca ctagcatcga caccatttgc acctgttttt 660 gtacaggttt ta 672 // ID FN619772; SV 1; linear; mRNA; EST; INV; 643 BP. XX AC FN619772; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-643 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; aa589055dc201986304dfb97312d48a9. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..643 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 643 BP; 273 A; 74 C; 117 G; 179 T; 0 other; gttagtgata ttttaagatc atacgtaaat atttagtatt tactcgtttt atattgctgt 60 ataatgtata atactaatat tgtttatcaa tgttggtaaa aaaaagcaat aatatatttg 120 gaatatatat aatataattt gaataatata aaagtatatc tctttcacac atacatctcg 180 tgtcttataa tctgctaaaa atgggtggtg gagatttgaa tttgaagaag tcatggcatc 240 catctactat gaaaaatata gaaaaagttt ggaaagcaga gcaacaacaa agtcaagaaa 300 aaaagaaaat agctgaatta aagagagaaa tagaaatgga gaaagatcga gaggatatga 360 aaaaatacgc aatggagcaa ggagtaatag aaaagaaaga tgacaaaaag ctagattgga 420 tgtataaagg accaaatcaa ttagtcaata gagaagaata tttacttgga cggccaattg 480 ataaatcatt tgaacaaatg gttcaaacag aaaaggataa tgaattaaac caagcaccaa 540 gaaatcatgt tgaacacgaa tgtattcccc cgtcattacg tttcttttct ggtaatgaac 600 aagtggattt agcaagaaag atgcaagaag accccttata tgc 643 // ID FN619773; SV 1; linear; mRNA; EST; INV; 721 BP. XX AC FN619773; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-721 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e430a8f7617b9f2045f9452e0b912292. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..721 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 721 BP; 225 A; 113 C; 131 G; 252 T; 0 other; agttgaacac cttgcaattt tatagtacgt ctagtcatag tatgccatta atgttttgac 60 gtgttatttt tttaaccgaa tagatagcga attagtcgcg aaaattggct gaaaacatat 120 aaatacttta ggtaaaatga tgcaagatga gcatctgggt ggtcctccaa gacgaaatgt 180 ttttagtcag caataggaag aatatcacag ttatatcaaa catatttgga tctttggact 240 cctcacgcag tgtcaagatg gacagtggct ttttttctag tatttgtttt ttctttacgt 300 atacttttat cagagggatg gtacattgtt acatatgcac tagcaattta tcacctaaat 360 ttattcatag cattccttac tcctaaaatt gatcctggaa tggattttga tgatggtgaa 420 ggaccagagt tacctacaag atctaatgaa gagtttagac catttattag aaggttacct 480 gaatttaaat tctggtattc tgtgatgaaa tctacaatta ttgctatgat atgtactatg 540 tttgattgtt tcaatgtacc agtattttgg cccatacttg tcatgtactt cataacgcta 600 ttttgcatta ctatgaagcg tcaaataaag catatgataa aatacagata tttgccgttc 660 acttatggaa aaccagttac cagaatcacg aggatacttc gaggttatac cttcaagtga 720 a 721 // ID FN619774; SV 1; linear; mRNA; EST; INV; 650 BP. XX AC FN619774; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-650 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ae00e5da508d96092ffba412c1b8490b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..650 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 650 BP; 233 A; 96 C; 128 G; 193 T; 0 other; catacacgct ttaggtacat gtaaatagaa cagttgagat agtgctactg tttaataggg 60 aaggatcgtt tagcgattcg actaattatt ttcgtatcag tttcttgaac ataaaacgaa 120 aaataagaat attttcttta ataaattttc gagggtaaga ttacttatga gtcttatgag 180 ttacaattag atgacgttga ctgcattttt catgcattag cctaccagct gatcagagcg 240 ttcattgcgc gtacaacttg tggatgttaa cataacctaa tagcttatga cacttcagaa 300 ttgtatgtgt ataacgattg aaaattggaa agttaaactg aaagaagata aaatctttta 360 tgtagagtac aaaaacgtct actatctgtt gaactgaaaa ttcatttgaa tgaagaaggt 420 taggcttaca agaaaggtag atagaattag tcaacacaac aaccaagcct tataaagatg 480 agtatctcct agtgaaataa taaacgtaat cttttgaagg cacctgtgca aaattaaaat 540 tgtaaccatg tctggaaaag gaaaacaaag ttccaaaaag gcggtcgtca aagtgcgaga 600 aagtgggaag actgtacagt cttctgccct taactcggat acgactttgg 650 // ID FN619775; SV 1; linear; mRNA; EST; INV; 698 BP. XX AC FN619775; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-698 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e2457b28156fd8cf25324b35339c5abc. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..698 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 698 BP; 214 A; 106 C; 111 G; 267 T; 0 other; tgatttttgt gtgtgtgtgt gtgtgtgttc cctggcaaac cgttacagaa ttgatcgaag 60 acatctttct atattatact cgtcttatcg cctgatacgc cgatgacggc tactcggtga 120 tcttttatat ttacgtatgg gactattttt ttcatagact ttgtcgctat ctttattatt 180 attacataga cgatagacgg ttggttaaag gcagaaattg ctggtattca gctaaaaatt 240 gatagaaaat acgcgtaatc aaagaaagat atttgtacat tatcttttct cctttatcat 300 ttacactttc atctatggat cactggatat ctatgaattt agaacaagaa cgaaatcata 360 ttatttatta taaaatgtta ttattatgga ataggtctaa ttttatcagg tttatattac 420 acaagtgctt cagtatcaaa tggtaaaaag tgagaaagaa atattttcta tctactaatt 480 atatgttgaa ataacgtagt tttccgatta gatagtctta ctctaaaatt tcaccgaaat 540 gatcggcttc cttgttgaaa gatgtccaaa atcaacattt ttacatcctc ttttctatcc 600 gctttacatt ttgttggttt ataattctgg aacatcttat agcagaaatg taaattacat 660 tgccattttt atgagtttac cagaatgtac ctgtacaa 698 // ID FN619776; SV 1; linear; mRNA; EST; INV; 697 BP. XX AC FN619776; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-697 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 66ddbd5047553ee8510bbc8c06a7964d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..697 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 697 BP; 220 A; 132 C; 133 G; 211 T; 1 other; gtattgacgg tgcataacct aacggtgtgt gatgtttcta acataaagta tgtacctaat 60 tgtcgtctaa aaattgaaaa tacatttgat taaatgatga gagatgcgta ccaagggcct 120 gctattgttg cttatctgca tagcaggaag cagcattatc ttcattgctt tcagcaatca 180 acgaccttcc attcaaactc tagttacaga gacacacaag caattacgaa acttccaaga 240 aaatttgaaa gacgttgaag aaaagcgctt ggtgacagat tccaagtatc ttgcaatgct 300 tgggctcgat gggcaaacga gcacaacacc ttttagtctt aaatctcaaa atgtgactgt 360 tgtctctctt atcaggcctg gaaacgaaca acacatttac ggctttgtga gaaatatcag 420 tcactttttg ccaaataaca gtattgtcgt atatagcgtc ggattgaacg acgattctct 480 gcaaagtatt aggacggctt gcaactctac aaaatgtaat gtgattcatt ttgatataag 540 cctatttccg gcccatgttg aagatgatag gctacatgtt tatagacctc tagtaatcca 600 gacagcttta aacacattag gaaatatact gtatatggat tctaatatgc gtttgaattt 660 ctcagacatt tcnaagtatc tttcaccgaa tctggga 697 // ID FN619777; SV 1; linear; mRNA; EST; INV; 651 BP. XX AC FN619777; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-651 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 69bf25705fe766659274118f8a17547f. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..651 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 651 BP; 215 A; 100 C; 123 G; 213 T; 0 other; ggcagcgttg aaggatgttg ttcctgtata taaaaatttg aaagaagaat gggcggcgac 60 tccgtgtaat ttgaagaaat gtggtgaatt acttaatcaa ttaaaggttg gtcttacaca 120 tttaatgttt cttccaacat ctaataacac agctagtcaa aatgaattac ttatagctcg 180 tgatatttta ggaattggtg cacaatggag tatagtaacc gaagatatac cttcttttga 240 acgttatatg gcacaattaa agtgttacta ttttgattac aaatcaggat taatggagtc 300 tgcatataaa taccatcttc ttggtttaaa tctcttattc ttactgtctc agaatcgcgt 360 ggccgaattt cacacagaat tagaactgtt gccttccgat caaatacaat ctaatgttta 420 tatcagacat cctttgagct tagaacaata tttaatggaa ggttcatata acaagatatt 480 cttagcgaaa ggcaatgtac ctgcagcatc ttacaatttt ttcatggata ttttattaaa 540 cactgttcga gatgaaatag gagcatgtat ggaaagtgca tatgacaaga tctctataca 600 agatgcctcg aggatgttga acttgaattc tgaggcagat atgaaagcat t 651 // ID FN619778; SV 1; linear; mRNA; EST; INV; 664 BP. XX AC FN619778; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-664 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ab40883c52c76f15e40ecb651d03ce8b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..664 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 664 BP; 202 A; 115 C; 152 G; 194 T; 1 other; cgatttcgtg aacagctacg tttacttcag tgtgtatcct acgaaaattt tattcagaac 60 tatgaagagt ttagttaacg aagttaataa tgccgtcacg gaatctcttt ttggattatc 120 gtttgcattt ccgcaattgg agtatcaaga atctcataaa gtcgttttga tgccaaacct 180 tcaagaacgg aaacataagg tatccctgat atgcggcggt ggaagtggac acgaaccttt 240 cgcggctggt tatgttggaa atggtatgtt agctgcagct gtagcaggct caatatacgc 300 agcaccacca tcaaaacata tctcttatgc catcgaccgt gtttctcaat ataataatac 360 tggtattttg atggtcgttc caaattatac tggagattgc cttaattttg gaatagcaat 420 tgaaaaagcg agacaaaagg gtctgaagat ttcggaagta attgtaaatg aagactgtag 480 tataccgaag gaggagcaag gtgtcgctgg caagcgtggt ttaacgggaa taatatttgt 540 tatgaaaata gctggtgcgt tagcagaaca agggctatca cttgaagaag tgactaanac 600 tgcacatcat gttttgcaag atatagccac ttattcggtt ggataacagc ttgtgcaata 660 cccg 664 // ID FN619779; SV 1; linear; mRNA; EST; INV; 689 BP. XX AC FN619779; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-689 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 5fa001200f4518012bda388fdc1ea03e. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..689 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 689 BP; 242 A; 130 C; 131 G; 185 T; 1 other; ctcagttggc tggcaagcca atagttttag caagtggtaa caaaaacgta ggagttggag 60 taaacagcgg tggaagcgta gtacttggaa agcaacaatc atcgcaacag acacagcaac 120 aacctatcat tttaccgagt caattactca acatcaaaac gatgcacggt ttgaaggtga 180 taccgacacc tgccggattg aaaactacgg gtgcggcagt ctacgcgcga gttattgctc 240 caaccaccat aacttcgtct caatcaacgg gaaatcaaca gcagactatt cagacgcaac 300 caccgagaaa taacacttac agtacacctt gacaaaattg atgtcactgt cttttttcat 360 ttatcctcgt tccaacttat tttaatctct tgtaattctg aattttctga cgaacacacg 420 atttatatgt ataaaaacaa aacgtaaaaa aagaagaaga aacgaaaagt gtaataggtc 480 agtaaagctt atacatagaa ttgagttata ttatgtacta tattttaggt ccggtaaaat 540 gaaaaaagga aaatcgtgca gttctttcga caggttgatc ggaatcaagg cacattgcta 600 catcangacg ttaaaatgaa aacacacggg tcgtgaagat ttttttatat taagtgtcga 660 attattttac aatgcatggt acgatttta 689 // ID FN619780; SV 1; linear; mRNA; EST; INV; 664 BP. XX AC FN619780; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-664 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a1bceb60a1d38d93e012a57a60f32757. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..664 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 664 BP; 215 A; 117 C; 97 G; 234 T; 1 other; tgtatttact taataacttt cnaaagcgta ataagaatat aaatactaca ggaaaaacag 60 atatcgataa tatctgtcat atttatttat gtgtaatgga aagccaatgt ttcatatctt 120 tcacagacaa gatttaattt cgtataatta aaaaagattc cagacatttg gatttattga 180 gaaaacgaga aaccattgtc gatctaaacg atctgtaact cgaccaattc taacaagttt 240 acatttaact acgacgagtg tttgttcgag ctgttgtatg tcaccggaag tatggtagca 300 tcttctctac cttcaattcc aattttatcc atatgtatat tcacgtttta tctaaaacac 360 ggacgaaaca tgtagtaaag tcgaaatata taataaaata acaaagaata cggtcagtta 420 tgatcttgtt gtgacattgt ttaagtgggt ttgagcgatc aattgacttt gctccagcta 480 tatatacgaa accttcttcg tccctactgt agtagctgca atctccttga atacaatctc 540 ctagtacgtt tctatttgac tttcattaag ctcttccatt aagctacgtc ttctctcatt 600 agatcttcaa ataattttta aatgcacgaa atcctgttaa tcctctgtag gatgttttac 660 tttc 664 // ID FN619781; SV 1; linear; mRNA; EST; INV; 684 BP. XX AC FN619781; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-684 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 4a37d20fb64e2e30d84cbd39e3587ace. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 684 BP; 227 A; 131 C; 127 G; 198 T; 1 other; ggaaacggat agtgctagct ctcgcttctc gtagaatttt tcccttaatt gagtaaacaa 60 tggcacgtac caagcagacg gctcgtaaat caacaggagg taaagctcct cgtaaacaat 120 tagcaacaaa ggcagcacgt aagagcgcac catctactgg tggagtaaaa aaaccacatc 180 gatacagacc tggcacggta gctcttcgag aaatcagaag atatcaaaaa tcaactgaat 240 tgttgatcag aaaattacct ttccaaagat tggttcgtga aattgcacag gatttcaaaa 300 ctgatctacg ttttcaaagt gctgcaattg gagctttaca ggaagcatcg gaagcatacc 360 tagtcggttt atttgaagac acaaatttgt gtgctatcca tgcgaaacgt gttacaatta 420 tgcccaagga tattcaattg gctcgacgaa ttcgcggcga gcgtgcttaa acaatatgta 480 attcccatat ataccattta ctactattat tattatcatt cgtaacagtc gtgaatgaaa 540 cattttcatt ccaaactaca tattatgttg tactccataa cagtgtacaa gttatgacat 600 gatcatcctt tatagtatgt atctaaactt cagatttctg gnaaactaga tacaaagatt 660 ggtataatct gtttttatat gtag 684 // ID FN619782; SV 1; linear; mRNA; EST; INV; 713 BP. XX AC FN619782; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C14 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-713 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 91ba1141b7df5ed0c40f878b5fd3ded8. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..713 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C14" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 713 BP; 248 A; 106 C; 167 G; 192 T; 0 other; ggcgaagtca tttcgtggac gaagtaagtg aggtgtattt ttaaaaagtt tgaaataatg 60 acagcaaggt acgatagagc tatcacggta ttttcaccag atgggcattt actacaagtg 120 gaatatgctc aagaagtcgt gagaaaaggt tcttctgcgg ttggcgttcg tggtaacgac 180 gtagtcgttt taggtgttga aaagaaatcc attgcgaaac ttcaagaaga acggacagtt 240 cgtaagatat gtctcttaga tgagcatgtt gtaatggcat ttgcaggttt aacagcagat 300 gcaagagtac taattaatcg agcacaagtt gaatgtcaga gtcacagatt aactgtagaa 360 gatccagtta cactggaata tataactagg tacattgcag gtttgaaaca gaaatataca 420 caaagtaatg gtagaagacc atttggaata tcatgcctgt tagctggttt tgattatgat 480 ggtgttccac acttatatca aacagaacca tcaggcattt attatgagtg gaaggcaaac 540 gcgacaggtc gaaatgctaa aacagttcgt gaatttttgc aaaaatatta tacaccagaa 600 gaagtagcga cagaaaaagg aacgataaaa ttggctatta gagctttact agaagttgta 660 caatcaggaa gaaaaaattt agaaatcgcc gtaatgcgac gtggtcaacc tct 713 // ID FN619783; SV 1; linear; mRNA; EST; INV; 670 BP. XX AC FN619783; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-670 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 66fac6fe894791cc27f150dbbec63330. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 670 BP; 238 A; 85 C; 127 G; 220 T; 0 other; gcgagaagta aaatcaacaa tttcagagac tggcacttaa cttaactgat atttgctggc 60 aactctgtat tggaacacct ggtagttcct tgagttcaac taatcgaagt tgtattgtaa 120 attgtgtaga gagatttatc gatacaagta actttattgc atatagatca agaaatacag 180 ttttaactaa tcctgcagaa gtgaatgtgc aataaattat ttcatgatta agacactttc 240 atagttaatt gagcaaaaat attgttacta tttatgatac ataacatctt gtaaacaaca 300 ttttatacaa tttatgtaaa aaaatgtttt caagtcttcg tggatttgtg aaccggcata 360 gacgaaagtt tatattggga ggtatcatag ttggcggtgt tatttttgtc agatatacag 420 cacgtagact tcgagaatgg caagagaaag aaataaaaga tatgctggac agatcaaagc 480 gacgtcaata ttttgaatgt aatgaaagaa catgcagtca gatgataatg tctcttacat 540 taactttaaa agattctata ataaaagttt tggatactaa tgctgttgta gataagctta 600 gaaatggttg tcctgataaa gtatcatgtt ggaatgaatt aaaagtgtta gcaattgcta 660 gatctgcctt 670 // ID FN619784; SV 1; linear; mRNA; EST; INV; 673 BP. XX AC FN619784; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C16 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-673 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 31271e6b89c29dcb49d0b6de1f78ead7. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..673 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C16" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 673 BP; 223 A; 116 C; 137 G; 196 T; 1 other; cgttttctga ctgccgtgct aaccgctatt tttaacgcat gacggcgtaa tactaaattt 60 gatagaaaaa atgcgatgcg ctctggccgc ggcaacttta catttttcga tcgagaaata 120 aacaaagcaa agtaattcat gttatgttgc aatgagcgat aaatcgacaa tggcggcaat 180 gtccccgtcg aagaggaggt tacagaagga gttaacatct ttaatacgtg aacctccacc 240 aggtgtacat gtagatgaag atcttactag tcaaaattta acacaatgga ttgttcacat 300 ggaaggtgca aagggtacct catatgaagg cgaacaattt caactgcaat ttagattcag 360 ttctaaatat ccttttgatt cgccagaagt gactttcata ggtggaaata taccgataca 420 tccacatatt tacagtaatg gtcatatctg tttatccatt ttaactgaag attggtctcc 480 agctttgagt gtgcaaagta tttgtttaag tattgtatcg atgttgagca gttgtaaaga 540 aaagaagagg ccacctgata attcctttta tgtaaaaaca tgcagtanaa atccaaagaa 600 gacaaagtgg tagtatcatg atgacaatgt gtaacagcaa ctgcaaaagt tataacaact 660 ttgtcattgc tgg 673 // ID FN619785; SV 1; linear; mRNA; EST; INV; 731 BP. XX AC FN619785; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-731 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; d562500d996e3ea6c935ae2c719556c2. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..731 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 731 BP; 308 A; 95 C; 108 G; 219 T; 1 other; gagattgatg agtctgataa acgaataaat ttaaacgtag gaaaaaggga aaacaaagaa 60 aagtacgtgc acgagaaaga aaatatctgt gaatgcgtaa cattaattta atttaagcga 120 aagtgcacgg aataaaatta taatgacgtt atatttatat ttaactcgtt aaaaagatta 180 aatctcgatt gtgatctaaa tttgataaga aagaaaaaaa aaaagaaaaa gaagaaaaaa 240 acaaaggaat atgtgcagac gtttatagct tgatcaactt tgtttcatgt ccactcaact 300 atgacgaaaa ctttagttta agcacagttt tatttaattg atcttcgatt aaaacttcaa 360 taataacaat aatattaata atcgtcttcc gtttgcaaac atatattata gtattaatta 420 tataacaaca atgataacaa aattggtcaa aatttcttcc ccaatcgtga caatatacat 480 atacactgga aatagtcgaa ttcattgata tacatcgatc aaagagctta aatacagtac 540 attgtaacga ttaatacaca tatatgtaca tgtgcgtgtt gaaactaaag aaatcgtatg 600 ctagaaataa caaatattac tcgcgcatag tttaatatgt aatctttaat aggattgtca 660 aacgcagcgg tgaaaatttt atatcagatg cnaacatatt aatgaacttt atatcatgca 720 cgatcaactg a 731 // ID FN619786; SV 1; linear; mRNA; EST; INV; 704 BP. XX AC FN619786; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-704 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 808ac729b4c171a1f8eb1670e2cb8051. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..704 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 704 BP; 240 A; 97 C; 142 G; 225 T; 0 other; attgatgatt ccgatagttg gttttacaga ttacgcgtca taattatcga agagtgtaac 60 tgcaagttta gtattcaaag taccatagtg ctcgtgtgaa atctataaaa tgaacgtagt 120 atcgtgttgg atattaatta gtgtattgga attagtaaca gcagaacgga acacaaatga 180 ctatatgaaa cgagaacatt gtttgatcag accatatcaa gtgtctggaa cgacaatacc 240 ttattgggat tttatgggta gtacgatgat tacgaataac tatataagat taattccaga 300 ctcacaaagt aaacagggtg ctatatggaa ttctgttcca tgttatgtaa gaaattggga 360 gctccaagtt cattttaaag ttcatggaaa aggaaaggat ttatttggcg atggttttgt 420 gatttggtat gcaagagaaa ggatgaaaac tggtcctgtt tttggtaatc aagattattt 480 ccaaggatta gctataattt tggatacata tagcaatcac aatggtccac acaatcatca 540 acatccttat atttctgcta tggtcaataa tggttctttg cattatgatc atgatcgtga 600 tggaacacat acacaaattg caggttgtga agcaaacttt cgaaatttag aacatgacac 660 acacatatta atatgagata tgaatgggat acattaactg tttc 704 // ID FN619787; SV 1; linear; mRNA; EST; INV; 644 BP. XX AC FN619787; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-644 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 384efef23c854e29b385441da4a742ff. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..644 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 644 BP; 208 A; 123 C; 153 G; 159 T; 1 other; caaaccgact aaagccagct gattcagtat gttaatgaaa atttcatctc gtcgagcgag 60 ctagtaaact ggacactgtc agactggacg aataatccat cgatcttgca acgcattcaa 120 gaacccaaat actacgagtg ggcgaaagat ctgaacgaaa tttggaagaa attggcgagg 180 aaggtgaatc ctgaagttgc caggcagccg gatagacata gtctgattta tgtgcccaat 240 ggtttaataa ttcctggtgg tcgtttcaaa gagttttact attgggacag ttactgggtg 300 attgaagggc tactgttatc cgacatgtat caaactgcca ggggaatgat agacaatttc 360 ttatacatgg tgcagaaata tgggttcata cccaacggtg gtagaattta ttacctcatg 420 aggagccaac cacccctaat acatctcatg gtatcaaaat acttggactt tactggggac 480 tacggttatc tgcgcaaagt tataccgact ttagaaagtg agtttgcctt ctggcaacag 540 aaaaggatga tcgacgtana aaaaaatggg agaacgtaca aaatgggaca ctatgccgtc 600 aacagcacga gacctcgacc ggaaagttac agggaagatt atga 644 // ID FN619788; SV 1; linear; mRNA; EST; INV; 745 BP. XX AC FN619788; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-745 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 31f339533c8d3b0fd073dc000908f798. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..745 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 745 BP; 232 A; 149 C; 171 G; 192 T; 1 other; cgttcctcgt gacagtgttc gacagacagc aaccatacca cctacgttct aacgacgtag 60 agaacgtaga gaaccgccac gattttctat ttttctcgtc gcgaataatc aatttatcat 120 tcgtattctc cacgacggtt tttttcgtcg tgttcgattg tcgtagccgc agtgtttctt 180 aagttctttc tttatgagta aattcgtttg ataatttcaa caagcaaaaa gaaaataaaa 240 cgttcaaagt aaaaaagaaa aagtaaaaaa agtaaaaaga gaatcgcgcc accatggccg 300 acgaacttcc ccctgaacaa attgcggtcc ttcgtaaagc gttcgacagc tttgataggg 360 agaagagtgg tagcattcct accgatatgg tggccgatat cctcagattg atgggtcaac 420 cctataacaa aaagattttg gacgaactaa tcgaagaagt tgatgcagac aaatccggtc 480 gtctggagtt tgaagaattc gtcacgttag ccgcaaaatt cattgttgaa gaggatgcgg 540 aagccctaga aaaagaactt cgagaggctt tcaggctcta cgacaaagaa ggaaatggtt 600 acatacccac tacgtgcttg cgtgagatcc tcagggaact ggatgaccaa ttgacggacg 660 aagaactgga tatcatgatc gaggagatcg attctgatgg atctggcacc gttgactttg 720 atgangtcat ggaaatgatg actgg 745 // ID FN619789; SV 1; linear; mRNA; EST; INV; 761 BP. XX AC FN619789; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-761 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a0700384d13ba62610d8dec8148345a9. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..761 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 761 BP; 247 A; 144 C; 102 G; 268 T; 0 other; tttattaact cttggttaac gtttgattaa tttttaataa tcttagtaat tcacagaaaa 60 atccatcaat gaaatattaa tcaaattcaa gaatattttt atcgacattt aaataactga 120 agccacaaca atattttcta aatcttttta taaacattta ctctgaccga ttttcacctt 180 ctagtagcaa atatttctta ttttatcccc tattgctttt ttatacaacc aaatcacatt 240 tatatgaaga atatttctgt atttgataag tttaatgtta tttcattgta tacattggcc 300 acgtcgtcta ttgaaatatt ccagtttcct tccactctaa taaagagtcg ataaaggtaa 360 cgtaataaat ccaccactga aaggaatcaa ttttccattg tacctttcct cttattacat 420 cacactttag ttttattctt gaccacattt tacatcgtcg tattcgatgc aaatcccatt 480 agttttctat ttcacggggc gaatccgata atattcattc agaaagaata gaaaatcttt 540 cgtcatattt ttccacgaac gtggaatgat ctacgttata ccataggagg aatcggagaa 600 ataacaaggc tgctgggaaa agtccgtagc gtgcacattc ccttccactt acgtaaaaat 660 ggtaaaggat tcgcgcgaag atataacgat tcgcgtacgt gaacacgatc acgtcacgtt 720 tcgtaaagta cagacgtacc ctcatattct gaatatttta t 761 // ID FN619790; SV 1; linear; mRNA; EST; INV; 669 BP. XX AC FN619790; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-669 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 6b3623321d5008d7f8961253b35598b3. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..669 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 669 BP; 183 A; 121 C; 157 G; 208 T; 0 other; gtcaggatac agaatacgga accgtcaaat acgaagatga actcatacca tcctggaagt 60 tttggaaaaa aagacgatac gtagtcggag tattagcatt ttttggattt tttacgtcct 120 acatccttcg tgtaaattta agcgtagcca ttgtcgcaat gaccgcgaag gttcacaaaa 180 tcgataaaaa tggaaccgaa tattttgaac aagaattcga ctgggattca aagacacaag 240 gactggtttt aagttccttt ttctacggat acataagtac tcaattactg ggaggatggc 300 ttggcgcacg tattggcgga aaaagagttt ttggtcttgg aatcgccact acagcttttc 360 ttacaattat cacaccacct ctagccagaa tgagcgttta cattttggtt gctttgcgaa 420 tagtggaagg aatttgcgag ggtgtaacat atccttgcat acatgcaata tgggcacagt 480 gggcacctcc attggaaaga tcaaaattag cgactcttgc attctccgga agtttctttg 540 gaactgtatt tgctatgcca gttgccggtt tgatggctga ataccttggc tgggcttctg 600 ttttttacgt ttttggagca gctggtcttg tttggttttt ttactggtgg ataatcgtga 660 agggataac 669 // ID FN619791; SV 1; linear; mRNA; EST; INV; 592 BP. XX AC FN619791; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C23 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-592 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 5720a034744c9a35a379035e45aaa966. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..592 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C23" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 592 BP; 176 A; 119 C; 132 G; 164 T; 1 other; tgtattcgat taaagccccg ctttcggagc agtgcacgtg cctcaagtac aaaatatcct 60 aatgccacgt ttggtgtatt tgcattaaga ggttcaactt cggtatttgg atatttggat 120 tagtcgacgg aaatttggag agcttcgcgt atcgagatcg tccgagctac gccggaagac 180 cagcggaagt gggaactata tatcctatct gtttctgact ttattgcgtg aatccacacg 240 cggtacgcta gctgattcag cgaacagcga aagaattgac atggactatc agattgaaac 300 agtcactaaa gatgataagc tcaggatact taactttctc aagaagttct tcttcagaga 360 cgaaccgctc aatcagtcgg tccaattgct atcaaataga gaagacttca cttgccgtga 420 tttggagaat tatagtctag cttcccttga gaacgatctc aatctgatgg cagtcttacc 480 gaacggtgtt ttaattggtg tagtattaaa tggnaagatg ggatcctcca gcgacgaaga 540 acacagttcg tagctacatg tcgaaaccaa aattcaaaaa aattcttaag ta 592 // ID FN619792; SV 1; linear; mRNA; EST; INV; 651 BP. XX AC FN619792; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051C24 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-651 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8d10ae9fa1af069395abe48a9fb6c94e. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..651 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051C24" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 651 BP; 186 A; 103 C; 124 G; 238 T; 0 other; gcgtgaattt gttatgtcgt tgctctagtt cttatttaca gttaaaagtt ttatttagat 60 tttatatttc atatagtttt atatgatcgt ataaagttag aattcaagag aactgaatat 120 ttgttatttg ctaagtagaa ttttcgtcat attttcatga atttgtactc tttataacga 180 tgaggcatat gtgcattagc taattacatt ctttagaaaa atggggttag gacagttaag 240 aactcggcca atgaagtgtt tacaagaaga ttgcgtaatg gttatgaaat atctgggaac 300 ggtattcgtg agatgttagc cggtgtgaat gtcactttca acttttcacc gcgaacacag 360 gatttctatt cccacacttt tcgagttcaa gttcgagttt gtattttctc ttgatatgca 420 aggagtccta tattgttcgc gaacgctggt ggagacgcac tttaacgcga tcgttttcct 480 gtaatgctgt tacaatcact agtcgtcaag agtaatcgta atcgacaagg attaaaatca 540 caaacacgag aaattgtctg ttgcactttt cgacctgtat atattctctg taccgatatt 600 ggtaatatac tcgtttggtc acttttacac atcattgtat acatataccg a 651 // ID FN619793; SV 1; linear; mRNA; EST; INV; 635 BP. XX AC FN619793; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-635 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 80cf15b75c58c14fe3d6b81da790f51e. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..635 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 635 BP; 244 A; 103 C; 126 G; 162 T; 0 other; acaacaatgg tgataaaata tattggtcgg acacatacct ttaagggcaa accactatgg 60 gagatattag gaaatttgaa gaaccatggc gtaggtagaa tcgtaatacg aagtgctcga 120 caaaaatatc ctggagcatc ttacatgagg ataatgaaag ttgttgccct accagatact 180 tcaaaacata tacatgaccc acggaaagtg gtggtattgg tggaaaaagt tttcagaggt 240 caaaaatctc ctacgctagt acagatggat ggagcaacct ataaaccaga ttacgtgttg 300 attcccaagg atcaagaggc aaaatatata aatgaaacta aagtaccgga gctaagactc 360 atgccacgaa caactgactt tcctcccctt ttgaaaaaga tattaataga gaagcaagga 420 aaagatactc ctgatttaaa actaaaaatt gtatatagta ctttaggcat taaaagttac 480 agagttgccg aagaagatga aactcctacg gttgatataa aagtgggatt aggaaaccca 540 gtaagtcctt gtttatacaa caataccaag cagaaggatg catcatgaat ataattcaat 600 atgtgcattg ttatcttgta aatttataat aaaga 635 // ID FN619794; SV 1; linear; mRNA; EST; INV; 731 BP. XX AC FN619794; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-731 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 948b21f8b2cb547151033ace2a404c52. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..731 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 731 BP; 265 A; 101 C; 146 G; 219 T; 0 other; acaatttatc gaaaaataca catgagagaa cctgtgaaaa ggaaacagac attgaaaagt 60 ctttctattt ttatttgtat ttattgaata tatatcattc ataaccttta aacaatagat 120 ttatattaca gcgtgaaaca tgtctaataa atttgaggct acttgcaggt taaaaactaa 180 cgaagacaat gggaatgatg aaacacaatc aaaagataat aaaaaaaact gaaaaagatt 240 tactagtttg caagcgtgga gcagttttgg agtctttaca gtcatttagt ccggccatca 300 gagttaacaa catctacaga ttttcatctt ttcaaagttg gcataaaacc aatgtgggaa 360 gatgaggcaa atcagaaagg tggtaaatgg atagtacggt taagaaaggg tttagtttct 420 agatgttggg aaaatcttgt attagctata ttaggagaac aatttatggt tggagaagag 480 atatgtggag ctgttgtatc tataaggttc caggaagaca taatatgtct atggaataag 540 actgcatctg attatgcgat aacagcacgt attagagata cattaaggag ggtcttacat 600 cttccaacaa gtgcctcaat ggaatacaaa actcatactg atagtttgaa gaatgttcat 660 cggctctaaa atcttgtgat gctaactcag agattcagtt ctttgtcgac ttcagctcat 720 agatactgtg a 731 // ID FN619795; SV 1; linear; mRNA; EST; INV; 672 BP. XX AC FN619795; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D03 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-672 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 25ebd22dcd3799fcce276a8a61a9a773. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..672 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D03" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 672 BP; 201 A; 106 C; 128 G; 237 T; 0 other; ttggctgtag acggaaagtt cggcgttgac tggagaagaa aattcccttg ttgtgtttga 60 atttgtttac ggcgtctata atcagcattc tccttatgaa atttgccgtt tctatacagt 120 aagttcggat cttaggtaac tgagtgtgtg ttaggtgaca agatcaggta tcaacatcat 180 catggacttt aatacaatga catggtgcaa ttgactcctt tcccgcttct ccattttatt 240 atccttcgct gatggactat ttgctgcacg gtcatctttg ttttcatcga cttcttatct 300 ttcatcgtac attttaactg agggtgaaga aggaagagat ttttcaagta tctaaagaga 360 aggtaatgct tcatacagta atgtaataaa atctagctca taattgtgga gggaagtgtt 420 gtgattttac tacaggctca tttagttctt cattctcttg gaacattcca agatggttgt 480 tgagagttaa gcaccatttt gcatttataa ttcacaaaga aaatatattt tcttttcatt 540 atacgttcat gctatctaat gagaaatggt aagtaattaa taaaatagta acattgggaa 600 caataaaatg accaaaggca aactattgca tagaaatgaa gaacatttta catatttgca 660 tgactgcctt tt 672 // ID FN619796; SV 1; linear; mRNA; EST; INV; 659 BP. XX AC FN619796; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-659 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b07c3a464eb6f1c5d98734f616b80461. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..659 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 659 BP; 183 A; 134 C; 127 G; 215 T; 0 other; agaaacgaat cgactgaata tttctaccac aaacttggat tttacacgtt tctagcgcgc 60 caaagaactg tacgaaatag aaaaagctgt ctctattaat cgttcgttac gattcgacga 120 ttttctgcgc tctctttctg tctctcaatc cttttctatc gcagaaacaa ttcgcagctt 180 ttccactttg tttctctgtt ttattttacc gtcgatacga ttcgattggt tgcacgcgcg 240 tcccaccgat ccagcttcga tcttacacaa agcactgtcg acagcgatgt ctttcgtcgc 300 gttagaaaaa tacactgtta gccacttttc gtcttcgcgc tttccgcaat tactagattt 360 gtctagtcaa aattgtggta taattttaag caccgatcac acagtatgta tcgcttgtaa 420 gcagcttgta ttaaaggttg agcaatacga aacctagagg cccgaatttg gcaaacttcg 480 tgcgaagatg cgtagcgata tatgcgatta acgaccttgt agaagactga atcaattaat 540 taaggattag tgtaaaatac gagtaagaat gggaggattt tcagatgctt gcgagtttcg 600 ggtgaatact ttttagcgaa atatttaccg tattataagt aagctttatt ttctatgat 659 // ID FN619797; SV 1; linear; mRNA; EST; INV; 675 BP. XX AC FN619797; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-675 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e5545681b6d8720dfbebe7a806bf2e43. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..675 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 675 BP; 244 A; 101 C; 94 G; 235 T; 1 other; tctaacctat tcgtcgtaat tgtcatgtac atataggaac attatattta tagatgtaaa 60 tacgaaagaa aactaggttc tttttaaatt tattcagcaa ttgttcattg ttattgtgtt 120 accatgaatg tacgaatatt taaaaaaatt caattggatt ttaaatgtaa tagaagtatt 180 tttgtgaaac atcctcgttc tattcattat atttccaatg cttctcccct aaatgtgtat 240 aagaatcatt ttttaaatag taaagtatta atgggatatt tttcaaataa gcggaatttt 300 tcaaatacac aaaatgctgt tttgcatcag caactccatc caaaagtatt tcaaagatca 360 ttactatgta acaaggatgt agtattaact tcaaaaattc ctagcaattg ttatcctact 420 gctgtaaata gtattagaca atattcaaat tcaggaccca cagttgtaaa tgaagttatt 480 aaatacaatg gtggcatttt ccaaatgata tccgaaagta tatgtgtaga atgggtcacc 540 gaagctttta gattcatgca tcatcaaaca ggtttaccat ggtgggcaag tatcatactt 600 acctcaatta ttacaagaac atttataaat ctaccattaa atatacttga tcttcacaat 660 anggcaaaac aggaa 675 // ID FN619798; SV 1; linear; mRNA; EST; INV; 716 BP. XX AC FN619798; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-716 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8a5b944c829d8be162b7d2a80236f2ef. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..716 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 716 BP; 223 A; 124 C; 131 G; 237 T; 1 other; agctaagcaa taaagccgag gaaattacgc agaggaggcg aagttcagtt tctatgaaca 60 ctcaacgagg tgagatctgt aaatattagg aatgagagcg tagatataag ttattttttt 120 aaaagaaccc tttgacttat atatcgagag tttccgtggt ttcgtcgatc gagcatcgag 180 agttacatct catctcgtta attttacgac tatattttta gaaattgttg taattccgtg 240 taaacaaatt tgcttgattt tatgattccg tagctttgga tacgacgttc ggttttgtta 300 attttctcac gcagatagtc gtaactttta tttgaatctc tttgaaaaag aaatgtttct 360 cgtactgtca ttccaatacg tagacagcgg atttttacgc attcgcggaa aattcgaaag 420 tataaaacgc gtacgatacg cgcagtatgc aaaaatatac atagaatatt caaagtgcag 480 taggcgttat aatatgtagt aggtaaatca aatttctacg taagtctaat ctctacgcgt 540 gaatttgcat aaatatcaac gaactaccga taaggcgtac gaatactttt acgaatgact 600 gtatgtgctt accagtgtag atattcgtaa tttaactgtt gtaatctacg ttattctacg 660 taacagccac cgtatcattt tcccatctac natctaccaa cacatttcat cttcgt 716 // ID FN619799; SV 1; linear; mRNA; EST; INV; 680 BP. XX AC FN619799; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-680 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 6b4f84f2ac0a79a37d4fcd594aedb2c4. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..680 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 680 BP; 262 A; 88 C; 136 G; 194 T; 0 other; tttatcaaca tatttgtggt ttgtgatttg ttacattaac agtttttaaa atcataggtg 60 tcgcattatt taagagagac agaaaatgga ttatttcgtt ggagaaatat ttgaaattcc 120 ttattttaaa gcagtatgga gtactatcgc atccgttgga tggtatataa ttggaattac 180 aatctgcatt tggtgggcat ctccgtatat tttggaaaaa tatgcgaatt ggaagttgaa 240 aaagaatgaa caggaatatg cagcaaaata tcataagaat ccagatctat tgcgagaaag 300 actctcaggt ttagaagctt cacgacaaaa aatgcaagaa gaatattatc agaaatgtat 360 gttagcagaa caggaaagga tagagaaagg aaatgcaaaa ggaataccaa aattaatctt 420 agacaataat gtcataggcg atagactagg aaagaagaac aacgattcac caccttttac 480 agagaagaaa cacaaatcta taagagagga atacaatcca ttaatgggcg atggttctag 540 aggatatcga ccccctaagc gcacttgttg cggaaagggt agttgcggat aattacatgt 600 aagcgtaaaa actaatataa ttttattttg tattcataat tatcaacaag aatacttgaa 660 aaaagaaatc gtttgtttac 680 // ID FN619800; SV 1; linear; mRNA; EST; INV; 694 BP. XX AC FN619800; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-694 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 431b7340428cf940594a0f4e20798675. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..694 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 694 BP; 227 A; 98 C; 108 G; 260 T; 1 other; ggggcagaac aaacagaaga agnactaata attttttttt aataatcgga gataagtttg 60 taacctttta cggcgatcgc tgtatattat gtggatgtgg cttaagagaa gcgttattca 120 ttaaccagcg tcgtaatcaa cattgacatt ttcgtttggt tacttttaat cggttgtgtg 180 cttagaagaa gcgaaaataa agtgtgaaga tccttccgta aaatccaatt tccgatttcg 240 gcgtgtatgt tattactttc ttctgtactt acagcatcgt ttcttataaa acatcgttac 300 atcatttttg catctatcct aaattgtttt taaggtatgg atattttatt aatctgtatt 360 ttctgtttta catacacaca catatatatt tcttttttca tgacaattaa taaagcgaaa 420 agattattgg tttagactcg ataccctgcg aacgtgtcat tttatttctt tttttcaaga 480 tagcttatta atcatcaaca aatacatggt tttaagacat cttaaaagaa gtatacacaa 540 aaaaaaagat attttttgat aagaagcttt taacgtagat tagtaggatg tagcgtttca 600 ctgtaatatt gcgcctgaat taataatatt aatagatagt ttaatacttt tatattgtat 660 tagatataaa caatcaactt tcacaaagta cacg 694 // ID FN619801; SV 1; linear; mRNA; EST; INV; 748 BP. XX AC FN619801; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-748 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; f7958abb87a050ee7acdcb87b50ff4fe. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..748 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 748 BP; 248 A; 119 C; 120 G; 261 T; 0 other; tgatatagtt tacttccttt cttcacgttc ctcgcatgag aacagcgtcg ttatattgtt 60 gccattggag actgcaatat tttctacgaa atcgcgaccg ataattagag ataatagaat 120 cgataaagaa tcgcgacgac tagcattttt tgtgcatgta attgcatatt cgccacattt 180 ttttgatatt cgtatattcc atttatcaaa atggttactt cgtcactttt ctcatcgcaa 240 agaattccgt aggaaaggta agccaacaat ggtttgcaaa cagatatttt tctcgcctgt 300 gagttccatg agaaaagaaa gtaaactata aaaagttact tattacctag tcaagtaatt 360 ggaaagtgat agcagatagg tcacattctc caatcaaatt tgtattgaaa ttactagtta 420 gttttacgat tacgtttttt ttcgaattac tttgtaaaat tccattgaag aaacttgaaa 480 atttttaaaa ctttaaccga gtcttgcggt gcatcgatac aaaataatca aacagtgact 540 gaaaggaaaa tgataatttt attacgaatt tggctgggta ttgaaatctg tttttctatt 600 acttaccaat tgcctgacta ggtaaggaat tgaactacga aaatatatat acacaaaaca 660 cttttctatg atatatgtct tttacaagaa ttaccttatc tttttacacg tcctctatat 720 taaaaagagt atgtgcggaa atatgatt 748 // ID FN619802; SV 1; linear; mRNA; EST; INV; 671 BP. XX AC FN619802; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-671 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 2c661654315cb008944094991f9b33f1. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..671 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 671 BP; 223 A; 108 C; 97 G; 243 T; 0 other; tacgcataca tgtttgcaca aattgccggt gtcgatatga cgaaccgtaa atggaaacgt 60 catccttgtt ttaaaactac ggctctgttc attctgtaga tccagcctcc cacaggatga 120 gcaagttgta tttgtgatcc cttcctcttt attgtgcagt acgattacga acaatgtttt 180 cttctccaca tttcatatgt ttacgttact tccttttttt gctaaatagc gatttaagag 240 acagtcatca tcatatcaat tacacaacat ttattacgta ttgcaaataa gttagattac 300 aaaagaaata aatttaataa ttacgtttcc taatgttgct tccattttta aatatgctac 360 attttaaaaa ccaagtaaga aagttcaaaa tcttaattat aacgacttat attttgtgtt 420 gaattaatac aagtgatata ttgtgaactc aacgtgaaag tttgcttgac caacagatat 480 gttttattag tctctcttag cacagattca tataaaagat tctatatttt tatgcgaagg 540 gatgagtcta tatagaatga aataatatat aaatctatac tatgtgtgcg cgtgtgtatg 600 ttacacatac actcaaaaga tgtaatacta tatgtgttat aatataataa atcagtctat 660 gtttcaatca t 671 // ID FN619803; SV 1; linear; mRNA; EST; INV; 674 BP. XX AC FN619803; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-674 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e1ec9f4b6f358bb9c344f80525b99b16. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..674 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 674 BP; 282 A; 98 C; 144 G; 149 T; 1 other; tcaagatggc tgctaccaac tcgaatatat ccagtgatag tccaactaag aaggaggacg 60 aattcaatga attttattcc gaagtgaaag aaatagaaaa aagagattcg gtattaactc 120 ccaaacaaca aattgacaga ttacttcgtc ctggttcgtc gtattttaat ttaaaccctt 180 ttgaagtatt acaaattgat ccatccacat ctatagatga aataaaaaag aaatatcgac 240 gtatgtcaat tcttgtgcat cctgataaaa accaagatga cgcagaacgt gcacaacaag 300 cattcgaaat cgttaacaaa gcatggaaaa cattggaaaa cgaagaaact agagctaaat 360 gcatggatgt tatagaagaa gcaaaagcta gaacggatca tatgattgta gaaaagaaga 420 aaaaattgaa aaaagaggga aaaacagaaa gtgcaaatat acttgaggag gaaacagaag 480 aaggttatag acatgcagtt tgggttatga caatgaaact ctttgctgac atggagagaa 540 gaagaaggga attagctact agagatgcgg aacaacgtan aaggaaacga gaagaagaac 600 tatctgcaga agcacaagct gtgttagaac gtgagtggca aaaaattttg aagaatctcg 660 acaaatcaga agtg 674 // ID FN619804; SV 1; linear; mRNA; EST; INV; 653 BP. XX AC FN619804; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-653 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 262f83900ba65ec684dbac291d3ba381. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..653 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 653 BP; 188 A; 110 C; 126 G; 228 T; 1 other; gtttagggtt cgaccaacgc tagaaccagc gcagcagtca aaattattgg ttttacgatt 60 ttattttaaa attcctgctt cgtgttacat atttttccgc aatgatgcaa cgacttttgc 120 agcgacgact aaaggaacga tataatgaat tgtatttttg tatttattca aaatcaaact 180 aattttgtcc tggctacttg taccgatacc tgtcgaaata actgctactt gtcaaaatgt 240 aaaagggtgc tgcaatctgt ttatcgaacc ccaattaacc agtttcctcg tttttttaca 300 acttcccacg cgtttttaga atttttactg tgtatcgttc gacacgatgg tgtcgattat 360 caggaaaatt ttggactcat cgtgatgctc gtttaaagca ggattttgat taggctgaga 420 tagtctttgc ctcgtatgga ccaagattac gaataatacg atacaatgtc gttgatattc 480 cgattttgtg atactatttg gcgattaaaa aggcgtactt ttatcatcgt aagcttaagt 540 aaagtcggat taatggaatt atcgatgatt cgcgaattgt tacaatctgt taaagaattt 600 tggttagaca gagatattcc ttgtttgcac gttgantaag atatcagagc gaa 653 // ID FN619805; SV 1; linear; mRNA; EST; INV; 694 BP. XX AC FN619805; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-694 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e584699404ffbcb4d0f6825a729a826c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..694 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 694 BP; 203 A; 143 C; 161 G; 187 T; 0 other; ggactcggca aggagcacct gcctgatctt ttccccaaag aagaggactc tgtcggagcg 60 ttgagcaaat atcttaaact tttcgctgaa cataaggtta atctgtcgca tatagaatcc 120 agaagctctc ttcgtcgaac gaatatgtac gagtttatgg tggaatgcgt gcctggcgga 180 gaccttggaa cagtgattga aaaattgcgg gaacaatgca gctacttctc gatcatttct 240 agaaatcaca aagataacat gggaactgta ccatggtttc caagaagaat cagagatctg 300 gacaagtttg ccaatcgaat tctctcgtat ggagcagaat tggacagcga tcatcctgga 360 ttcacggatc ctgtttatcg acaaaggcgc aaatactttg cagatttggc gttcaattac 420 aaatatggtc aatcgatacc aagggtggag tataccaaag aaggaatcga aacatggggt 480 gtagttttca gaaacttaac gaagctttat ccaaagtatg cctgcaagga acataatcac 540 gtgtttccct tgttaatcga gaattgtggc tacagagagg acaatattcc gcaattggag 600 gacatatcaa actttttaaa agattgcacc ggtttcactc ttcgacctgt ggctggcctt 660 ctatcttcgc gcgacttcct agctggtctg gctt 694 // ID FN619806; SV 1; linear; mRNA; EST; INV; 728 BP. XX AC FN619806; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D14 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-728 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; bd4cff0c5c11006684ad0134acee32a5. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..728 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D14" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 728 BP; 243 A; 110 C; 149 G; 226 T; 0 other; gtgagagttg tatgactgct ttagagtaac ggagtgtttg tgtttgctaa ctttttgacc 60 aatttattta atcgcctatt taaaaacaga atgaaacctc taatgttgca tgggcacgaa 120 cgtgccataa cgcaaattaa atacaataga gaaggagact tattattctc ctctagtaag 180 gataaaaagt caaatgtttg gtattctttg aatggagaac gtcttggatc tttcaacgga 240 cacaatggct cagtatggtg tattgacatt aattgggata caacaagatt tttatctgga 300 agtggtgaca attcactaag ggtttgggat tgtcaaactg gaaaagaaat aggtttgctt 360 catacaaaca gttcagtgag aacatgtagc tttagttatt cagcaaatct tgctgtttat 420 tctacagata aagctttagg acaccaatgt gaaatgttta tcattgatat caaaaatgtt 480 gatgcagtat tatcacaagc agatgcaatt tctagaatcg ctgtgaatgg tcccaaaatc 540 actgctatct tgtggggtgc attagatgaa acaattatta ctggccatga agatggtgaa 600 atcactcttt gggatgttag aacaagaaaa aagatgaaca gtgtcagaag tcacaatcac 660 aaataaatgc atgcattttc acaagatgca ctatgtcgtg actgctcaaa gatatactgc 720 taactttt 728 // ID FN619807; SV 1; linear; mRNA; EST; INV; 695 BP. XX AC FN619807; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-695 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 785aac368daa00b1fd8e5174fce27c11. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..695 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 695 BP; 203 A; 112 C; 163 G; 217 T; 0 other; taattagctt ccacattgaa cagtccagcg gaaagagcac ttattagaat tttgaaaaat 60 ggcatatatg atggataatt tagttcaacc taatataagt tcagttgtca gtaatttggg 120 cccagattgg ctttcttctg agcaaagtac aggattattt cattaattag acatcaatca 180 tggcttgtga atttgatgga ggagttgtaa ttggagcaga ttctagagcc actactgggg 240 cttatatctc caatcgtttt gctgataagc tgactaaaat cactgatcac atttattgtt 300 gtcgttctgg ttctgcggca gatacccaag ctatttcaga catagttgca tatcatttga 360 gtctgcacaa aatggaattg ggcatggaac ctttagtaga aacagcagca aatgtgtttc 420 gtgaattatg ttacaattac agagattcct taatggcagg aatcttggtg gcaggatggg 480 atagtcgtaa aggaggtcaa gtttatagta ttcccttagg tggtatgtgt gtacgacaac 540 caatttccat tggagggtct ggttcaacct atgtgtatgg ttacatggat gcacaataca 600 agccaaacat gtcaagggat gaatgtgtta aacttgttga aaacacattg gcattggcaa 660 tgtctcgtga tggtagcagc ggtggtgtca tccga 695 // ID FN619808; SV 1; linear; mRNA; EST; INV; 689 BP. XX AC FN619808; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D16 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-689 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 16a1982aca09bb027a7ddc6142973f9f. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..689 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D16" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 689 BP; 226 A; 111 C; 135 G; 216 T; 1 other; actcgtgatg gagagagttt tccttgttcc gtttcacgat caactgagtt tcgagagtca 60 cgcgcagaga gagtttcttt cttcgatcgg tttctttagt taaataaaat gattggcatt 120 gtgaaagtag ttggaacttt taattggaaa gcttgcgaga ttttgtcaga ttgcaattga 180 gatcgtaact ttcgacaagt tataaagcta tatttttaag caagtaggta ttgaagttct 240 gaattatttt ctgaattctg aagtcaagga aattacgtat tcgaaggcaa atgacttgaa 300 tagtaaggac gaatgtcgaa atttgaaaaa atatttcaag ccagagaaat aacagattca 360 ccgatgcatt cacttcagag tttagtttct actatttgaa gcaatatttc acctactggt 420 ttattctaga aggttgacaa aagtggtctt ttgagagaaa caagggtccc gatcagatct 480 gcttagaaca gatagactta ttccactagc tccactgtca gtagtacgag tcgtctttag 540 tcagaccatc ggaacaggaa atattcccaa aataaagcag gtacatctaa ataatcttct 600 gcgcggtaat aaagttcaaa acgttaagat taatcgtacg aagtcccccg tgncaatata 660 tcataaattt caaaatatta aacgtatta 689 // ID FN619809; SV 1; linear; mRNA; EST; INV; 730 BP. XX AC FN619809; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-730 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 950f8f508af678450f33fb7b9f23d557. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..730 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 730 BP; 217 A; 153 C; 173 G; 186 T; 1 other; gggttttctg tacagtgatt acgaacgaca acattattca aggatttatt gccgacaact 60 ataacaatat tcacgatgga atcattacga gagcgcaaga gattacttcg tcaagagttt 120 tttgaagaag atgaaaactc gcactccgaa gtggatagtt ggatgtctac aaaaccaaag 180 aatgagtttg acagaccgag ttgcacaatt aatctggacc cacattcgga agtatataac 240 atgaatcaca aacgaagagg tatcgcgtta atcatcagcc acgtcaaatt caagaaactg 300 gatacacgag ttggtgggga aaaagatttg aaggaactgg agcaaacatt aaagtacctt 360 ggctttgacg ttcgagttca cctcgacttt aatgttaaaa cgatattgtc gacggtgcaa 420 aacgctgctg cggaagacca cacggatgcc gactgtctgg tcgtagctgc tatgtcgcac 480 ggagaatcgg gtgtcctgta tgcagccgac aatgtgtatc cagtggaaat gctttggagc 540 cctttccttg gcaatcgatg caccagctta gcaggcaaac cgaaattatt tttcattcag 600 gcttgccgtg gaacgaagct ggacaatggg gtggaaatta ttcatgagac ggattccacg 660 cccagtacta cctacagcct ccctgcctat gcggatatta tggtcgcgta ctccacttac 720 gatggntttt 730 // ID FN619810; SV 1; linear; mRNA; EST; INV; 709 BP. XX AC FN619810; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-709 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8001390e5e157163dc72401cf59ef216. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..709 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 709 BP; 225 A; 118 C; 127 G; 239 T; 0 other; gatggcgtag tattcgacgc gctaactgag atacaggatt gtagttctga acaaattgcg 60 tgatctaaat ctgtatgata cgcatcacgc agttctataa tatggaaaaa ttcttagttt 120 tcccgcgcgt attttgatgc tcattgaaca ctttaacgga ttttttttag caccatttat 180 atcatatttt gtatttatag cgataataca tatcacaaat agatagtttt agtcaacgta 240 taattacgtg agtcgtattt aatctcatat tctgaattct gtagaaatgg atctgccttg 300 tgattggcag accaacaaac aaagactttc agaacgcagt caatatttat tagagacagg 360 acaatggtct gactgcaaat ttgtagttgg tcaagaaccc catcaacaaa cattaaaggg 420 acataaatta tttttagcta tgtctagccc tgtctttgaa gctatgtttt ttgggggtat 480 ggcagaaaaa aatgacctga tacctattcg agatgttcaa ccagaagctt ttaaagctct 540 tttagaatac atatatacag atcgagttga tttaagttct tttgaactag cttgtgaatt 600 atattattgt gccaaaaaat atatgcttcc tcctctggta aaagaatgta caaagtatct 660 atgttctgac ctgtctccaa aaacagcttg cagagcatat gaatttgct 709 // ID FN619811; SV 1; linear; mRNA; EST; INV; 683 BP. XX AC FN619811; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-683 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 7e9d07b67f8b6f8283e157cfb714c723. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..683 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 683 BP; 191 A; 136 C; 175 G; 180 T; 1 other; catgatatct gggaagatcg gatgtacgga ttctttttga gtattatcaa actgaccaga 60 ttgatctaat cagttgatct aatcatgttt atgtgcagtt taggtcacga attgcacaaa 120 ttttaacaat ctggaaatag tatttaatta attaccgaat cgtggtactt cgatcgagtt 180 tgtaaacaaa gaatggccgg ggagcctcgt gaaaccgggc atacgaagcc gttggtgcag 240 cttttcccaa aagcatccga gtgcgtgtat acgagttgtt attgcgagga aaatgtatgg 300 aagctctgtc aagatgtagc gaccagacac ggatcggaat tacagcattg ctatgttgct 360 ttcgtgagca actcctggcg tagcgtaccg ctttggcgac aacgtgcagg aaaggacgag 420 gataagctcg tagtttggga cttccacgta attcttatct acgcacccga cgaaagggcg 480 gttgtttacg atttagatag cgcgttgccg tttccgacgc atttctggaa atacgcgatg 540 gaaacgttta gatcggacga ggttctacag ccagagcatc acagaagatt cagagtgatc 600 ccagccaatg tgtacttgcg agaatttgca tcggatcgac atcatatnga acgcgaggat 660 ggaaacatgg atcagacgcc acc 683 // ID FN619812; SV 1; linear; mRNA; EST; INV; 723 BP. XX AC FN619812; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-723 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 7c40a624e2a3a8ef85d7f051cc1b8b82. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..723 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 723 BP; 230 A; 131 C; 165 G; 195 T; 2 other; cataaccgtc gtcgggcagg tagactttca ttcgctgttt ggtactccga ggagaagcat 60 taacggttgc tcgtgtttaa gaaaactttt aagaaagtat tataaaagat gtcaggacgc 120 ggtaaaggcg gaaaggctaa gggaaaggcg aagacccgca gtagtagggc agggctgcag 180 ttccctgtcg gaagaatcca tcgtctttta agaaaaggaa attatgcgga acgagtggga 240 gctggagcgc ctgtttattt ggccgctgtg atggaatatt tggcggctga agtactcgag 300 ttagccggga acgccgcaag agacgacaag aagacgagga taattccacg tcacttacaa 360 ttagctattc gcaatgacga agaattaaat aaactgctct cgggtgtaac aatcgctcaa 420 ggtggtgtcc tgcctaacat tcaagcagtg cttttgccga agaaaaccgg tactggtgga 480 tctggaaagg gagacaaaac atcccaggaa tattaataat cattagaata acctttctaa 540 cttaacagta ctttagggct atatctttgt ttcttgagcc attaaagaaa aacaaaataa 600 tctctggaac ctatcaaatt accatcgctt catcnaacgt tcattgtaaa attattttag 660 ttaacagtat aatctactat tttattactg aataacatct tttatttnag catagtgtaa 720 taa 723 // ID FN619813; SV 1; linear; mRNA; EST; INV; 682 BP. XX AC FN619813; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-682 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 6e8ff16acd8ff7587cd8f2fccd194b51. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..682 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 682 BP; 246 A; 127 C; 127 G; 181 T; 1 other; aattccattg caccaaacac agttgctaat aagtaaggca gttggtgcgt ctcgatataa 60 tgaaaaacga acgattaaaa ttcatttgtt tcaagtttcg gaaaataagg tgttctaaag 120 caaaattcag acattacttt gcggaaatgc agtgtataac ctttcggtaa ccatgccaat 180 aaattaatgt ataaaagaaa agattcctgt acatggaaat aatttaaatt ccacttagtc 240 tggataatgt atttaattaa attaacgtat cattaggaaa tatataacag aaaaatgtct 300 gctggaacag aattattatc cacagcggaa ccacatctta ttccaggata tgccggatac 360 tgtcctcagt atcgctacag atgtggtgaa acgtatggaa gtcttacgca caaagtgctt 420 cttgatccta cagtgaatca tgcggagacc ctcattttat cgaaccgtat ccccgacgat 480 tacgaggtcc agaggccacc gaaaaatgac atagacgtag taaatgctag atacaaaagg 540 accgacccta tatttattca tcctataaca ccaagttacg aaggatttac tccaaaacta 600 aaagccagaa atggacagag atacacggtg ttggctacag aaggacttgc cgaatttttg 660 acgacacnat taagaaatag ag 682 // ID FN619814; SV 1; linear; mRNA; EST; INV; 753 BP. XX AC FN619814; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-753 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; d1b9debd69910d299d03b9572054b44c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..753 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 753 BP; 273 A; 117 C; 103 G; 260 T; 0 other; taagtgtgat gagtaaacac attcaaagaa tcatcaggta tttaacaact aagcttatat 60 tctaaagtcg aagattgatt taattaagat ataaaatttc attaagaaaa aaagaaagat 120 atacaaattg cttactcacc aagaattggt ttcttcaggt ggtacgaata taaatcatga 180 ataagcacga agatatcact gtttccactg ttctgataaa cagttggtta atttgccaac 240 aatcaaaatc gcacaataat cgttcaatga acatttaatg caatccgata atagacaata 300 cttataacgc aatgcttatt gagctgtgca tatacagatg cactgaagaa acgatagagt 360 tacgttttgc agtgattaat tgatgctgat agttacataa ttttcaatgt taagtatcta 420 cattcaggta tacatctatt ctaaacatct gtcacaatca caatgaaata gctctataca 480 ataatgagtt gtttttctca tcacatctca ttagacctaa agctgacctt gttcacgact 540 cacgatactg cctgtgcgtt acttaagata tattacttta agtttaattc ttattgaata 600 actttgcaaa cattgcatac aatattattc gcacaattat atgattaatc atttattata 660 ttttaagtca gatatacttg acttaatgga tttttgcagt aaattgctca ctaaatgtat 720 actgtacata tatataattt acgttacgta ttt 753 // ID FN619815; SV 1; linear; mRNA; EST; INV; 595 BP. XX AC FN619815; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D23 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-595 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e88905b56756528c20372d2a99849339. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..595 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D23" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 595 BP; 169 A; 106 C; 156 G; 164 T; 0 other; catggacagc cgttctattt cgaagaaaga aagtttgtcg gagacggagt cttcaaagcc 60 gaattaaacg agtttttaac tcgtgaactt tcggaggatg gctattcagg ggtagaggta 120 cgtgttactc ctcatcgtac agaaattata ttattggcaa cgcataccca gagtgttctc 180 ggagaaaggg gtcggagaat cagagagtta acttctgtgg ttcaaaaacg atttaacttt 240 aaagaagcac agaacatcga gttgtatgct gagaaagttg cgacacgagg tctttgcgct 300 atcgcacaag cagaatctct gcgtttcaag ttaattggag gtttagctgt acgaagggct 360 tgctatggag ttctgcgctt tattatggaa tcaggagcaa agggttgcga agtggttgtt 420 agtggcaaat tgcgtggaca gagagcaaaa tccatgaaat tcgttgatgg tttgatgatt 480 cattctgggg agccaaccaa cgaatatgta aacactgcaa cccgtcatgt ccttcttcga 540 caaggtgtac ttggtatcaa agtgaagatt atgctgccct atgatcctca tgggc 595 // ID FN619816; SV 1; linear; mRNA; EST; INV; 606 BP. XX AC FN619816; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051D24 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-606 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; f8362860c695232ad439c08bfc888766. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..606 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051D24" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 606 BP; 201 A; 101 C; 127 G; 176 T; 1 other; ctgccggcac tactttccga cggtgtatcg aatagcaaaa tataatacca gtgttaaata 60 cgtaccccng tgttttaagt aaaaaagttt gtataagaca tcaaaaataa tcaaaatggt 120 tctaaacgta ccgatgataa aattctacaa tggcaacgaa gtgccgtcat tcggacttgg 180 tacatggaag tccaaacctg gagaagttac tcaggctgtt aaggatgcaa ttgatatagg 240 ctatagacat atcgattgtg cccatgttta tggcaatgaa aaagaagtgg gtgctgctct 300 taaagcaaag ctcaccgaag gtattgtgaa aaggcaggac ctttttatta ccagcaaatt 360 atggaatact ttccatagac cagacttggt tgaacctgct attaaaaaaa ctttatcaga 420 tttgggtctt gaatatttgg atctttactt aattcattgg ccaatggcat acaaagaggg 480 ggatgatctt ttcccaaaga atgctgatga tagtcctgct cttagcaatg tagactatgt 540 agatacatgg aaagctatgg aagctttggt atctaaagga cttgctaaaa atattggtat 600 ttagca 606 // ID FN619817; SV 1; linear; mRNA; EST; INV; 722 BP. XX AC FN619817; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-722 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 130f3595b180cf8505688d240af856a0. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..722 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 722 BP; 311 A; 98 C; 120 G; 193 T; 0 other; gatggattaa acagaaacac gacgtgatgt aaagattaca aagagtattt cttgatatta 60 aagaaatttc ataagattaa ttacaaaaaa gtgtataata ccctttatag caacaaacaa 120 tacattaatt taaaaacatt acggataaat agtaaagact accatccaat ataaatagta 180 aaaatgaatc aggcgaaaat tgataagtta gtttctaggt taaggtacaa tgttggatca 240 aaaccacgca aattaaagaa catagatggt ccagaaggaa gattgcgaaa aattaaaaaa 300 acattaactg ctgtaattaa atatgaaagg attgagttaa attatgcaag ggcagatgag 360 acaagaggtt acgttgaacg attaatatca gaagccatac gtcatggtcc tcaacataca 420 gaaacaatgg accttgcaaa gttttggata atagaaaaac aacttatcca taaacttttt 480 aaggttcttg taccaagata tgaaaactat acaacttcat ttacaaagct ccataatgca 540 ccatgtatat atccgggcta tgcatataaa agggctgttc tagaattgaa agggaatgta 600 tatccagtta tagaacaatc caatccatat caatataatt taattcacaa tatattactg 660 gatgcagcta gaaaagagta cagaatggaa aagtataaag aaatagctga taacatgaag 720 tt 722 // ID FN619818; SV 1; linear; mRNA; EST; INV; 699 BP. XX AC FN619818; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-699 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ca01db1370f129ba99162ace8ec57061. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..699 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 699 BP; 249 A; 100 C; 104 G; 245 T; 1 other; tacgagcgat tattgtctgt attaatttat ttatcatttt aaatatactt gacggttttc 60 aacagcgaga tatctcgtta ttcgagcaaa ccaaccaacc tctacataaa ccacgacaaa 120 gcttgtcaaa tacagcatgc gatgtgaata tagaatgtta agattttata tgatttcttt 180 acattgttat ttacatatgt aggaaacgtt atgtaaattt tctatttaag gaaatctttt 240 aatgatattt gacagaagga aatttaacag aaaattttct gaatatagcg agaatttttc 300 tcgaatgaga acaagtttcg aagaacgtcc agtgaaataa aaaaagttag ttttactttt 360 acttgtttca ttgcattaac aataaaatat agaaatgcga aaaaaagaag gaatttttat 420 tataaaatat gcataacaag cttcaagagt tcgtcacgta gattattaac attgctgctg 480 ttagagatat cataataaca gaaaatatta ttaactgatt gttatcttct cgtcttttct 540 cgtaatacag ctgtagtgat ttgcatctct gcaaatcgtt cctttgcaca atagtctatt 600 aaattttaat taaatcgaat aatttcattc catccataca aagaacggaa ttncacggga 660 gaaaatgagt aggttctcat tgtcgatgaa tctctactt 699 // ID FN619819; SV 1; linear; mRNA; EST; INV; 653 BP. XX AC FN619819; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E03 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-653 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ff252b053ab5b888b46031e2b8e975de. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..653 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E03" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 653 BP; 223 A; 95 C; 98 G; 237 T; 0 other; tagttcagtg gctgatttaa ttgactattc gatgaatttt agtcaatcag cagttttctg 60 ctattcacgg cctaagtatc ctggccatcc atctttccct gttagattaa caaagccagt 120 aagcaggttt actcaagtcc gaagtttaca atacctttgt cgttttgtta tacgtcaaaa 180 tactagatta gacaatattc ataagttgcc tttaccaaaa acaatcaagg gttacataga 240 agaagcgcac tattaacatc ggcctcacaa aaaatatttt ttacattgta aatgtattgt 300 tattgataac acaaagtttg tattgcatag caataatagc tttttcagaa aaacgtattt 360 ccgagaagta acaaaagatt agatatgagc ttctttttat aagaactgaa atgttcattg 420 ttaagatttg caagatatct ttacttttat aaaaattaat tgatataatt gtatcacttt 480 caagaacatt gataatgaac aatatgtgaa agatttaggt ttacatgaat cacttcttta 540 caaagtacgt tacatttttt aacgtaattt gattcgattt atgaagtact tggctgtgtg 600 gtgtgtatct gtataaaaaa atatattttt ttataataaa agtaatctcc tgc 653 // ID FN619820; SV 1; linear; mRNA; EST; INV; 684 BP. XX AC FN619820; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-684 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8b417b6f09bc205c20503c8fcf54e08d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 684 BP; 216 A; 149 C; 134 G; 184 T; 1 other; aactaggtca actacgtcga acagggcatc tctctgtacg aacgtttttt actcttttca 60 tacgcagaat ccactgtcaa tgccggacgc cccctatgca taacataaca tagtaaataa 120 ttgaccgttc gaacgatact ttgccgatct ttacaaactt cgaataaata caaataaatt 180 gaaagtagca tttcgcgaag acatcgcggt tgcaaaaacc ggtgaaactg aaacaggtgt 240 cgtagagcaa acggctaacg gcaggtatcg ctggaaattt taaataagct cgttgcaaaa 300 tttcctacga atcgtacagg ctgtgataat cacgtacact attatacgcg atcgtacgaa 360 atgtgacgaa tggtgcgaat acaaagtttg tacgtgacaa caaacggaaa acgatggagc 420 aatccgttgt cttgcgattg tactttgaca aaagtatact tatcatgttt tattattcta 480 atgaaaatta aataaaagca acccttgcgc cgatgatgcc cagatgtata acgttgaccg 540 gctgaccatt tcgatccatc gatcgtgaat cttagaaatt tatacgcgca cgttgatcgt 600 tgtctcgaac tggcagaaag cctgaaacta cttttcctaa taaacgcgcg tacaacgtct 660 ccacgctttt cgtgcnaact ctgg 684 // ID FN619821; SV 1; linear; mRNA; EST; INV; 685 BP. XX AC FN619821; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-685 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; aec85e25f532fe2bf173d2d1efee4b96. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..685 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 685 BP; 264 A; 85 C; 128 G; 207 T; 1 other; tatgatatgg ttgctattcg cgcattgttt atgtttgtgt cgttagtagt catcgcagaa 60 aattcaatat ggtgtccgtc gattgaagag ctaaattggc gttctgtata tggaaataac 120 attaaaaata aagaaatcgt ttaactcggc gtaattatga aaattcgttt gaaaagctag 180 tgtgtcattt ggaaatactg atgtcatagg gatatattgt ataaaaaagt aaaaattgtc 240 atctttaaaa ccctgtactg aaattgtatg ctttaagaaa agaaattcag atatatagaa 300 aataaaaata ttaacaaaaa attaaagatg gaaaggctta aaacgaagag tgtagtggat 360 ggaaatgaat cagagggtga acaaggtagc attgaagaaa agaatgaaaa ttctttaaca 420 gatattgcta ctttggacat tacatctcga cttagtctga aaacaactga aatatatgaa 480 gaatcacata gaaatggaat acctctagtt aatggtattt gtaattcttc tgaaagaatt 540 accagcatag ttaatcttcc acaatctgaa tctatggaaa ctagtcattt aaaccaacac 600 ggtacattag cagaaacatt gtcattggaa agtagggata ataacattca atatgttagt 660 tatacaagtg aactccnaat gcctg 685 // ID FN619822; SV 1; linear; mRNA; EST; INV; 558 BP. XX AC FN619822; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-558 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b264c7601efb5302a4951ecdb6018972. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..558 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 558 BP; 193 A; 91 C; 109 G; 164 T; 1 other; gacgtttata tacaggcagc tgcttacgtt agatcaaaat acagtgctct tagtgattgg 60 cttgcacgac taagctaaat ttcaattgaa ttaggaaaat accgattatt cttgtatttc 120 ataaactgta ccatcaagct gtaaggaaca caatgacgga tttaacaggg ttacgaatga 180 acgtggccgc tctgaaacgg gtagacccct atgtaaaaga tattctggag actgcgacac 240 acgtagtact ttatactttt aacgcagata ataatgagtg ggaaaaaacg aatattgaag 300 gtgctctatt cgtatactcg cgaaatggcg aaccctataa cagtgttctt atcatgaata 360 ggaaacatgt tttataatta aagattaaat acaaataacc tagtagagcc agttacgcaa 420 ggcttagatt tacaactcca agaacccttt ttactctata gaaattccag atgtaatatt 480 tatggcattt ggttttatga taaagaggaa tgtgtacgca ttggtgcaat gttgaacaaa 540 cttattaaag aatcngag 558 // ID FN619823; SV 1; linear; mRNA; EST; INV; 639 BP. XX AC FN619823; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-639 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 9e5bdcaeab34ee737be918934aef812b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..639 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 639 BP; 247 A; 93 C; 144 G; 154 T; 1 other; ctgatttcct agcaaattta ttttcttagt tttattactc gtttagcaaa ccggcggcat 60 atgtattcat aaaatccgac attttctacg tacatatgat cttatactgt tcattattcg 120 aacgacatta gcgagtaata tttgaacgac ataaagcgta aaatgaaacg cataccgacg 180 actgcgcgta attaaatttc gaaattaaat tagatctcct tacatttgat tagcatgctt 240 tcagacaaga cggatgacgg gtaaaaaagg agtgcggaaa tgtatatcag tgaaagcatt 300 tgtatagagt atagactgta cacgctgtgt atagactaag gtgaaagaac aagaggagac 360 gaaaggtaaa acatgattaa taagaaagta aattaaaaag cggaatcgta gacaaagact 420 agtggcagag agaaaataac aacggagaag tatatgggaa aattacttcc aaagcaagaa 480 aagaaagcgt gaagtgaaac acgagctgga agaaacgaac gaaatgtagg tataagacag 540 aacagtaaag aaatacagga caggaaaggt ttgtgagtat gtgcacgtga atggctggca 600 ttgagaagcg tncagaagcg cagctgcaac gcaatactt 639 // ID FN619824; SV 1; linear; mRNA; EST; INV; 673 BP. XX AC FN619824; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-673 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 61271c1653e6d91e20775934cb222ff1. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..673 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 673 BP; 216 A; 125 C; 137 G; 195 T; 0 other; ctttcaagtc cagacactgc agcatttcct tacttggaaa atcgagagtg tactttgggc 60 ggatatatga cgacgattgc cacgttttat agccgtgaag gaaacaggag ctttccagtc 120 atcatttaca ttgccaccaa taagaataaa cattggctcg gtgaggcgcc gcttcagaat 180 atcgctaaac aaatttctga atgttccggc ccgaatggac ataacgtcga gtatctttta 240 cgattagcag agtttatgca tcgttatcta ccagaagccc aagacgagca tttgtttaca 300 ttggaattat tagtacgttc gaggataaag gaaacgaata tgtgtctgga tacgttaatg 360 ggcaagcgag acttcaatat cgacctggag gacgtcgaga tcgatgccaa ggatgaggat 420 agtgacttag tcaccaataa cgcaaccaat aatctcagga aaaattcctt ccaattcagt 480 ctcaaagtac ctgataagac attgcgttgc ctcaaaatgt agactgcatt tcctatttat 540 atcgataatt atcgaatgct tggaccgaga tgtaaagaat ttatgtaatt aatgataata 600 agataatatt cttcgtagat aatttatgta aaaacgttcg tatctttcaa aaacgtgtgt 660 ctattgaata atc 673 // ID FN619825; SV 1; linear; mRNA; EST; INV; 735 BP. XX AC FN619825; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-735 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; fe3b0f1b6a2875b6994fe7920eab2465. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..735 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 735 BP; 234 A; 138 C; 151 G; 212 T; 0 other; cctcccgtta atgaaagtct ttttgaaagc tctgtagtac gattagaata attaactctt 60 tgcagttcag taatccctag actacagata tttatacatt tatgagatat tcgaagatat 120 ccggaatttg gaatttaaaa atgttcggaa cgcgtgttca tagtctgtag agatacacag 180 aatatcgaaa ggcttccatt gcgactaaaa tttcttcctc ggaacccgtc aacgcgaaaa 240 ctgagaacta cgcgtgacac cagcgtggtg tcatcagtgg tcaacgtgtc gatacttgtc 300 acgtgaaacg agcatctctg ctcgcaaaat caagaaatct gcgataaata tctacagtct 360 agtggttacg tttcatctag gaatataagt aaagcgacaa atgcgacgaa catctagttt 420 ttaaaaaccg tgaatcaatg tacaagctca atctgtaaac gactgcaaag ggctaagcga 480 aagttaaacg tttgaaattt cgaatttaga tactttagga attgaaatac ccattggtac 540 aagctcataa gaatagaata gaagagtcta aggtgaattt gtaccaatcg gtgtgcggag 600 tctcttgttc gtaggtcgat catttcgacg atatgtttct aagtgtttct caccacttcg 660 gactctagaa tccatgaatt actgtaccgc aaatggggag catcaggatg ttgcattatc 720 cgtagagaac ctgtt 735 // ID FN619826; SV 1; linear; mRNA; EST; INV; 713 BP. XX AC FN619826; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-713 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 36c465d3e84f52af5f3facfb7bb2bf87. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..713 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 713 BP; 211 A; 102 C; 131 G; 269 T; 0 other; acgtcgtcat actgagttgt acgtacgtgt cgaatttggt gttatacata ttaattatta 60 tatcaacgta aaaattacaa aaatgtcaga cgtaaggaat tggattaatt ctttaccaat 120 ttttacaaga tattggctat tatgtacaat agtttgcaca ttagttggaa gattcgggtt 180 aataaatccg actttactaa tacttataaa cgaacggttt ataaacaatt ttgaaatatg 240 gagagcagcc accagtgtgt ttttctaccc tttaaatcgg ggttcgggat ttcatttttt 300 gattaactgt tatttcttat ataattattc attgaggtta gaacgtggag aatacgatgg 360 acgaccagca gattattgtt tcttactctt attcaactgg atatgctgcg ttatcatagg 420 agttattggt gaattacata ttcttatgga tcctatggta ctcagcatat tatacgtttg 480 gtgtcaattg aataaagatg ctattgttaa tttctggttt ggtacacaat ttaaagcagt 540 atacttgcca tgggtattgt ttgcatttaa tctcattatc tctggaggtg gcatgatgga 600 actttttggt attctagtgg ggcatcttta tgtgttttta aaattcaaat atccctcaga 660 actcggtgga cctgaattta taatactcca aaaattctga aatctatttc cat 713 // ID FN619827; SV 1; linear; mRNA; EST; INV; 687 BP. XX AC FN619827; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-687 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; bd1e4cf354284341d35e014cd3daa50d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..687 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 687 BP; 210 A; 119 C; 162 G; 195 T; 1 other; tcacagttag tcggtgttga ttttgaagtt tatgggagag tacaaggcgt attctttaga 60 aagtatattc aaaaacgtgg gaccgaatta ggtttaaaat tcactggcga agttgtggcg 120 aacgctggtt tcggcatcga aggtcactgc tttgaagatg cacgaaatgc aaattccttc 180 gatcaacttt ttggagatac attcgcggaa tcattagtat ctataatcag attctacttt 240 ccatctatta atcgcttctt gaagatcaaa ttcctgccta aacgattgga ccaattcttt 300 aggaacaccg tcacagaaaa tctggaagtc agaagaaagg aactagtacc taggaatgat 360 ttcctgcaat taatgatcga catggaaaaa gcaggggagc atatcgatga agaggttgta 420 gcggctcatg tagtttcttt cttcgttgac ggagtcgaga catccagtgt tactttacgt 480 ttcgttggat acgatttggc tattcatcca gacattcaag ataagttgag aaacgaagtg 540 atatctacga tcgagaagca cggaggtttg acgtacgagg cattgaaaga catgacgtat 600 atgaaatcag tgattagcga atctcaaaga tgtcacgcag ctatatgcta catgcantaa 660 gtgtgcacag aggagtccga gtttgca 687 // ID FN619828; SV 1; linear; mRNA; EST; INV; 714 BP. XX AC FN619828; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-714 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 6c79a20392880b6bf08f27790032d39b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..714 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 714 BP; 232 A; 111 C; 136 G; 235 T; 0 other; gtagtgtagt gttctggaag tagtagtggt cgttcagttc tttaccagtg gagttacgaa 60 atctttatta attaataaag aattattata tatcaacaat ggagattcaa atattgaccg 120 cgaagtcatc aaaattcata gcaaatgtat ccgtgaaacc tactacaacc acacgagaaa 180 ttaaagaaga attatttaaa ctaaaaaaag caccacatgt tcacagacaa tgtttaaggc 240 tagatactaa gggaaaggct ttatctgatt cagatacatt gaagaattta tctatttcta 300 gtggtggaaa attatacttt aaagatcttg gaccacagat tagttggaaa actgtatttt 360 tagtggaata tgctggtcca ttatttctat atttatggat atatcaacgt gcatggattt 420 tttatggtga tgcaactgcg tctgaaatta ataatgtagt ccatgtggca gcagtttgtt 480 ggtctataca ttatgcaaaa agacttctag aaacattgtt cgtacaccgt ttcagtcatg 540 caacaatgcc actgcgcaat ctctttaaaa actgttcata ctgttggctg tttgctatgt 600 atgtagcata ccatgtaaat catcctttgt atacagctcc gaatcaatgc agtttctgac 660 cggactagta atatttgcac tttgtgaagt gggaaaactg agtattcatg tggg 714 // ID FN619829; SV 1; linear; mRNA; EST; INV; 700 BP. XX AC FN619829; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-700 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ad90ee310c111447233b3d8c5f683caa. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..700 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 700 BP; 238 A; 84 C; 126 G; 251 T; 1 other; tacatagtaa taaagttgta attattctct ttatgaaagg cttcagacca cataaggtgg 60 atttgtaatt cattttaaac aatattttta aatagaatta cgcgtgaaag tgttataaaa 120 atcgagtata taatcgatct ttaaaaagaa gagaaaagtt ttattaagtg ggaaattttt 180 cattgagaga atagaaagaa tggagaagat caaggtttat atacgtaaaa tatattgcgt 240 tttcagtgtg ttaccacata gttaggtatc aatttacacg aatgccgaaa gaatttgagg 300 tttcgtgctg gacacgggaa cacgattcca tcgtccttcg tagtcatctt gtatacctcg 360 aagtgggacc atttgacatc ttgtactggt caacgatgat gacataaaaa tgaagacgat 420 cttaatttct ttcagtggtg gttaaaagat ctcttacatt atttactgtt atcttttaat 480 ttataaagtt ttaagattta tttttcaaat aataaaaatg gtatcgtatt tttatagcat 540 ttttgtaagc taattattga cgtggataaa aacatatatt cctgtaatta attagcgtgt 600 tgaatatttg taatgataga taattagtaa aaatggtaaa ctttgtatgg ctttgttctc 660 gggtaagttt caccaagtac ancataggtc ctgagccatt 700 // ID FN619830; SV 1; linear; mRNA; EST; INV; 704 BP. XX AC FN619830; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E14 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-704 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 2fb443e97f31b9a6e2ab71399969dd13. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..704 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E14" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 704 BP; 264 A; 95 C; 108 G; 237 T; 0 other; ggtcattata cgcgacagtt taaacacaaa tagtagcaaa gcttaataca aattggtgaa 60 tcagtataat ttggtacact ttaacgtaat gggccgaaga aaaagtaaac ggaagccacc 120 aagtaaaaag aaagctattc aaccgctaga tacacaattt aattgtccgt tttgcaatca 180 tgaaaaatca tgcgaggtta agatggataa gtcgaggaac acagctagaa ttacttgcag 240 agtctgctta gaagactttc aaaccacgat caatttactt tcggaacctt tagatgtgta 300 taatgattgg attgatgcat gtgaaagtgc aaactaaatt ttattatctt tgtatcaggt 360 caatgctaat agtattattt atttcttcta aatgtaaatt gtcagcactt tagtataaat 420 ggatatcaca tcatgtgcaa tgcataagca acaacttagt gctatacata tattctatga 480 atcaatgaaa agacatttag tttcataaat ttaatatttc attagtttaa agtaacaaaa 540 tttgatatac atatcccata tgtagtgtat tcaatattat attgtattat ttttaatcat 600 acatgatatt tgtataatca tatttatata tatgagcttg tcgtacttac taagtaagga 660 tatgaatgtt tacattaaaa ataacaaaaa tattatatga ctgc 704 // ID FN619831; SV 1; linear; mRNA; EST; INV; 665 BP. XX AC FN619831; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-665 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; d99e30358d166f1dc34951c25ecf0c77. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..665 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 665 BP; 256 A; 88 C; 120 G; 200 T; 1 other; aatgttctaa tttaatacat tacttgtgct tgatcaaaat gttataatgg aatcggaatt 60 atcggaggaa ggcgctttac gctcacaatt tacagtggat gatttggata ttgaaatatt 120 gccacttata tatgaaataa ttcggagtgt tgagaaagat ccacatgaca ctacacagaa 180 agctaaagag tcacaagata caagtcataa aatattagag cttcaaaaga aattagattc 240 cacaagggca cagattagaa gattgccagg aattgaatat agtaaagaag aacagttgca 300 gaaacttgaa actctacgta aacaacttag gcttaaaaga gagcttttat tgaaatatcg 360 taacatgtgt acatttgaag tcccaaaagt ataaataatt ttgcttgttt aagaaaaagc 420 aatttatatc agcatgggat ttttacgcat gttatttaca tatttgatgc gtaatgaaaa 480 attaatcgag aaattagcaa atacaaaacc aattaggcag tctgcacgat ttgtagcata 540 tttgttactt cacagtagaa cacatggtat tcatttatct tttgatcgtg aaaaatttgt 600 taggcaatta aggctgttta caaatacatt tagacaaaag ttgncagaag ctaaagatga 660 aatga 665 // ID FN619832; SV 1; linear; mRNA; EST; INV; 656 BP. XX AC FN619832; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E16 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-656 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ca3e47515d5712f3005625049e029707. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..656 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E16" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 656 BP; 203 A; 111 C; 113 G; 228 T; 1 other; attgcagtac atattttgta actttccaat tatacgtaat tctactcggt aaaattggta 60 atgtaagcca ttatacgtta gtgtatgcca tctgatgttt actaaaattt ctttgtctac 120 aactgctaaa gtgttacttt ctctacctac attaagaaat atacgaagtt attctgttat 180 gatgagtaaa tttgatctcc ctgatcgctt taaaggcaat gataagtcag tatgggtgga 240 atatcttcag ttggcttcaa aatataagcc cataaattta ggacaaggat ttccagattt 300 ttatgctcca gaaaatttaa ctaaagcttt ggctgctgtg actacaagtg acaatcctct 360 attaaatcaa tataccagag gatttggtca cccacgttta gtaaatgcta ttgcaaaatt 420 gtattcgaag ctcttaaacc gtactttaga tccaaataag gaagttcttg taacttctgg 480 tgcttatgaa gcattatatg ctacgttaca aggtcacaca aactctggtg atgagtggat 540 aattattgaa cctttctttg attgttatgt acctatggtt agatctgctg gaggaattcc 600 aaagtttatt gccttanaac caaaaagcac aaagggatct ctttcttctg cagatt 656 // ID FN619833; SV 1; linear; mRNA; EST; INV; 726 BP. XX AC FN619833; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-726 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 5fc04c1175bf694def4fafc8fb41d4d3. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..726 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 726 BP; 245 A; 110 C; 144 G; 227 T; 0 other; ggttaattga aagttagtgg ttttcacgtt cgtgttctat gtatatatgt agcgcttaaa 60 gttctaaaac agacaacaat ttaaatatcc ctgaaggtca ccttttatga acattatgtc 120 cattcattgg ggtatagcag gtgctggaaa aatatcacat gattttgtaa catgcttacg 180 aacattacca gaatccgaac atgtagttct tgcagtagcg gcacgtgaac tatcaagggc 240 acagaacttt tctagcttac ataaaattaa aaaggcgttc gacaattatg tagagctagc 300 ggaagacaag aatattgatg tcgtatatat tggaacgtta cgtcctcaac actttgacat 360 tgcaaagtta atgttaaatc atgggaaaca tgttttatgt gaaaaacctt taactatgaa 420 tttgaagcaa acaacagagt tgataaattt agcaaagcag aagaagttgt tcttaatgga 480 aggtatttgg agtagatgtt ttcctatata tgaaactctt aaaaaagaaa ttgaatccgg 540 aagtattgga gatgttcatc aagttcttgt atccttcgga tttagaatgc cagatgttca 600 acgactcaac ataaaaagtc taggaggcgg taccatattg aacttgggcg tgtatggaat 660 ttcattttgc tgttaatatt taataacgaa acacctcata ctgtacaaac tgctgggtgc 720 ttaaat 726 // ID FN619834; SV 1; linear; mRNA; EST; INV; 738 BP. XX AC FN619834; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-738 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; aa28e9ac5cf42146f76f142454ef719e. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..738 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 738 BP; 263 A; 122 C; 123 G; 230 T; 0 other; tgctatttaa tattgtttta tgaaattttc catgtgtggt gcgttgtgtg caatgaacta 60 atagatgcaa aaaaatatag tacaaaaaat atagacaggt caataaagta acatgctaca 120 accatcgtat ctgagatagg cttgctaatt atactttgag aaagagtact caactatttt 180 aagtttatat tatagtagta ttatatatca gcaatcaaac taatagctct tacaggtaga 240 actgtacgtg ttcctatatt taatataaaa ttgcctctgt tatataagct ggtttatata 300 aataaatttc taaaatgctt atgtgtcatt taaacacgga cacataaata ttataattat 360 aattagaaaa caaaagagca caactaaata cgagacacta ttgtaagtga acctatgtac 420 acgcgaataa tctatcgcgg tcattcgctc catcaacaga aaatccgtag ctaccatatt 480 ataccgtaat gatagaatcg ccaatttcat ggtcaattta tcgccatttt tgatatcgct 540 gtgataacgt cgagcgctgc tttgaaataa acgatgctac gtaaacagct gcatcgctat 600 ctatgaatac caataggctg acacaaacat aggcttacgt gagcggtcag aggttacgcg 660 agtgtaataa tagaaatctt gacatagata gtagagctca cgccctgatg acttctcacg 720 atagaaattt tttagtat 738 // ID FN619835; SV 1; linear; mRNA; EST; INV; 704 BP. XX AC FN619835; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-704 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; aa9cbda05c727a77dccc2d2bcd36307d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..704 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 704 BP; 247 A; 123 C; 121 G; 213 T; 0 other; gttcgtgctc gttcagtgat cagtctttca tcggaaagtt ttggacagaa cgagtacgaa 60 gcaaaaccgt ggttaaagat gtatctgact cctgcaaccc tctatgcttt tagattatca 120 ttccaacagg gaactgtttc gtctgtgctc cgtagaaata cgatcgaacg tcataccacc 180 aaatcagcat gtttgcttgc aattattggc ctatctatga acgtattttc tgtgaggaag 240 ttgctagcct caaaacacaa tagtcgtttg taaaatcgtt attaaagtca gcagcaacag 300 atcaatccga aaaaaaagta aaaaaaatga agagaaaagg cagcgtaatt gtttgggacg 360 aaagattctc accaaatatt agaatattac acaaactccc ttgattgtat tatatagcgg 420 aaatcagaaa tctcatatta tcgcgttgta ttccgcaaat gttaatcttt ttctaacgaa 480 gtacattaac tacattgaac atacgtgcgt tttcacgttt taatgaatac ttcaaacgaa 540 aaacaaagac ttaaagtaga aataactata aaaatataaa acgtattatg taatttaact 600 taagcgatat aaatcgacaa ttatttcgta tttgcgcact ctttatgtat tcagttaaca 660 tgccatgtac agaaaaacga atgcgattcg ataattttgg atgg 704 // ID FN619836; SV 1; linear; mRNA; EST; INV; 660 BP. XX AC FN619836; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-660 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 9a9649400f378a1e056aeac4b4abab2f. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..660 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 660 BP; 175 A; 149 C; 157 G; 178 T; 1 other; gtagcgcgtc gttcgaagct ctaggtaacg attcaacgtg taattgcttt taattcctaa 60 cacgcgtgtg tctacgtatt agacaaaaac tctagtgatt ctcagtgacg tatatggtct 120 tatctcaaat cagccacacg atgcgtaaca gagctgaaga gatcgagccg tgtggcgatg 180 tagtgcatgg ccggtgaaag acacgcaacc tttatcctgc tacgtgttta tattcgcctt 240 tgtggtcatg gaaacggtga tcttacgctt cgtcctggat aatcgtcgta aacgcgccta 300 gtgaacgttc cttggcaacg tgcttcatcg agaaaccggc cagtttcttc gagtttcgac 360 ggccagttcc aagccacgct tcacagccag acgaaatcat cgtgactatg taacgacctg 420 tcaagggata ttgaattatt gatgatctca tgaattgtca ttgctccaac catgagttat 480 tattaatcgc tcgagatcga taagaagaca cgtatctatc gagaacatcc agcagtgaac 540 gtccataatg gctttggcaa agatcaagag ttttatagac acggggctga agaccgtgtc 600 cacgccgtta gatcgcacgg tgaatctgga accggaagtg ggcattanga tcgttcattc 660 // ID FN619837; SV 1; linear; mRNA; EST; INV; 683 BP. XX AC FN619837; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-683 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0d31706c0f84dafcc0b548c966ec2800. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..683 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 683 BP; 192 A; 132 C; 129 G; 229 T; 1 other; gatattcgac ttgatacatt cgaatctttg cggctccatg aagattctat ctgttggaga 60 ggccaagtac tttatcacat ttatcgacga taaatcatat tgcgagttaa ttgccaacgt 120 aggcgaagga ctgcgtgttt tctttaattg cgaccagtgg atgtaaaaaa aatgatgaat 180 aaagaggatt ctccgcagat cattgaacct acctactcgc ttgttttcaa ctttgaaaaa 240 aactactttt atccagatac tcaaatatgg ctccagaaca attttcagta ctgctactac 300 tgctgtatta tttacgtgat cctaattttt ggcgggaaat attacatgtc gagtagacca 360 aagtttgatt tgaagggtcc gcttgcatta tggagtggat tgtttgcagt gttctccatt 420 atcggatttt ctagaacagc accggaaatg ttctctacat tgagacacca tgggttttac 480 cacagtattt gcatacctag caactttttc caagatcatg tttgtggctt ttggacatgg 540 gtatttatcc tatcaaagat cccagaattt ggcgatacga tcttcattgt gttacgaaag 600 caaccattga tatttctaca tttttaccat catttaactg ttgttcttta ctncctggtc 660 acatatacgg agaacacagc agc 683 // ID FN619838; SV 1; linear; mRNA; EST; INV; 693 BP. XX AC FN619838; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-693 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 5dc68885e03f6848473c4875ed0c356b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..693 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 693 BP; 244 A; 91 C; 100 G; 257 T; 1 other; taaatgctga accgtgctag aataacgacc cagctacagc tcgaattgag tataaaattt 60 gaactaattc aaaaacattg aaaatgtaaa catttgtgat attaatatta aatagtcata 120 gtaaataatt cattatgatc tattaattat gaggttaatg actcgacatc caaataaagt 180 gattttcttt catgtgccat aatgaagtta cgattattaa ggatatctgt tgttaaacgt 240 cttttttgta caatttcttt ctttttttta ttaacttagg cgataaaaag atttaaaagg 300 cgacacagag aaaattataa aattattttt agtatccttt tcacaatcat cattttgcta 360 ttgttctatt actctcacat tttataaatt aaaaaaagca tgttactctg aaaggaatat 420 gtgtgagttt tttactaact ttgacattgt aagagtttgt actcgttttt atctcttcag 480 tggaaagcag aaacttttgt ttattaaaca agcttattta tatagtgcat caacaaaata 540 tttgtaatat aaaaacttta tgaccgatgt tataatgggg gataaggtta ttcgagcata 600 caaggagaat gtttggtgct gactaatatc aaacatatgt cacaaatatt gttttaaatc 660 gacattngaa ctttccctcc atatatgtac atg 693 // ID FN619839; SV 1; linear; mRNA; EST; INV; 650 BP. XX AC FN619839; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E23 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-650 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 58eb22d55bdb78fac63904beca932328. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..650 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E23" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 650 BP; 216 A; 101 C; 127 G; 205 T; 1 other; tggacctgtt atcgtttatc caccgatctg ctttcccagc gaatcctaat actgtgcagt 60 ttaagcttta ctttattgta aatatttagt atacagttct taattaaaaa aaatgtttca 120 ggacggcata tgcgatgctg ctgacatttt aaataatgtt cctcctaaaa agacaattca 180 tactgacaat gtagatcttt ctgataagtc attacaggtg gatatttcag atgctcttag 240 tgaaaaggat aaagtaaaat tcactgtaca tacaacaact acattaccag aatttcaaaa 300 accagataac ttagttgtaa ggcaacatga agaatttgta tggttacatg accgattcga 360 ggagaatgaa gaatatgctg gttatattat tcctccttgt ccaccacgac cagattttga 420 tgcatctcgt gaaaagttac aaaaacttgg ggagggtgag ggtaacatga cacgtgagga 480 atttaataaa atgaaacagg aattggaggc tgaatacttg gcaacgttta aaaaaactgt 540 tgctatgcat gagatgtttt tgactagatt agcacatcat ccagtttttc gcaatgatca 600 taatttaaga gtgtttttgg aatatgatnc agatctttgt gtgagaggga 650 // ID FN619840; SV 1; linear; mRNA; EST; INV; 612 BP. XX AC FN619840; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051E24 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-612 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 353319284f9eb729faf13f801bbb7c1d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..612 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051E24" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 612 BP; 209 A; 92 C; 144 G; 167 T; 0 other; ggcgaagtca tttcgtggac gaagtaagtg aggtgtattt ttaaaaagtt tgaaataatg 60 acagcaaggt acgatagagc tatcacggta ttttcaccag atgggcattt actacaagtg 120 gaatatgctc aagaagccgt gagaaaaggt tcttctgcgg ttggcgttcg tggtaacgac 180 gtagtcgttt taggtgttga aaagaaatcc attgcgaaac ttcaagaaga acggacagtt 240 cgtaagatat gtctcttaga tgagcatgtt gtaatggcat ttgcaggttt aacagcagat 300 gcaagagtac taattaatcg agcacaagtt gagtgtcaga gtcacagatt aactgtagaa 360 gatccagtta cactggaata tataactagg tacattgcag gtttgaaaca gaaatataca 420 caaagtaatg gtagaagacc atttggaata tcatgcctgt tagctggttt tgattatgat 480 ggtattccac acttatatca aacagaacca tcaggcattt attatgagtg gaaggcaaac 540 gcgacaggtc gaaatgctaa aacagttcat gaatttttgc aaaaatatta tacaccagaa 600 gaagtagcga ca 612 // ID FN619841; SV 1; linear; mRNA; EST; INV; 670 BP. XX AC FN619841; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-670 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; db6efbf4b4ecc9de82919f131b5eb40c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 670 BP; 211 A; 129 C; 142 G; 188 T; 0 other; ggaaacatag caataaaagg agacgaataa taaaattctc tgcacgaatg aaaacgcgat 60 cgtggccgcc tcgacgatac tttcggggat cttgaaccgt cattttcgaa cgttgacgat 120 ggatcatcgt ggaggcgatg caacgtaggc gaaggtaacg cgcgatattt cttccgctaa 180 ttgacgcttc tcgtggaatt agcctcggat gaacgttcgg gcaagtctct tgatgcgttt 240 cgtatcgcga ctttcaacgc gggtgttccc tgcaaagaaa acatagtaag gtcgtacgtc 300 gatgaacgtt tgaaagatta tcgagatacg tttcgttcta ttttaaatat cactcgaata 360 taaatctttg actaatctgc tctacgtgag atactattgt acgtttgaaa aataaaattt 420 cgaataaggt agttggtaat attttaacga tcggataatt cgaaaaaggc cttcccatgt 480 ttctacgtac ataattatac gaacttgcct cgagagactt atcacgaaca aataacgtaa 540 tcttttaatc aaacagccat taaagctagt ataatgtaca caccttgatg ccattatgtc 600 atatgaataa ttaaaacgaa gctatagagt caagcagtgt catggacacg cgatatcgcg 660 cgagatacgt 670 // ID FN619842; SV 1; linear; mRNA; EST; INV; 695 BP. XX AC FN619842; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-695 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a634aca55daadafdf8130146282a58fa. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..695 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 695 BP; 247 A; 92 C; 121 G; 235 T; 0 other; gtagcaggtt gtgttatttc gaagcactgt agtcgagtgt tgatttgaag tgagtgctcg 60 agaagtggaa taaatcgatt ttcataacag gaaagttagt tttaaaacat cgcaacaaaa 120 tgaagaaatt tagcattttt ttattactct tcatttctgc aattactttc acaacgaata 180 aagtttatgc tcaagaagat gaaaatatag atgatatcgt ggatattgaa ggagaagata 240 attctgtaat aacagatgaa gaaccagaag atgaaacaat cactgcttca tctgatgctg 300 atacaattat attatttact aaaccagtta ataatggatt atcaacattg gaattaccag 360 ctggaaatat agttgagttt cttgttggtt tcaccaataa aggagaatct gattttattc 420 ttcaatcttt ggatgcctct ttccgttatt caatggagtt taacttttat atccaaaatt 480 tttcaactgc tgtatataac aaaattgtaa aaccagaaca cgaagcaaca ttagcatatt 540 catttattcc atcagaaaat cttgttggaa gaccttttgg tttgaatatt aatctaaatt 600 ataaagattc taatggtatt gactttagtg aagctgttta taatgaaaca gtgcaaataa 660 tagaatcaga tgatggtctt gacggagaaa caatt 695 // ID FN619843; SV 1; linear; mRNA; EST; INV; 724 BP. XX AC FN619843; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-724 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 4d0bf05e83d758ce46350debb1c6b500. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..724 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 724 BP; 253 A; 88 C; 159 G; 224 T; 0 other; gtgcatagtt gctaagaagt ctttatcctg ttaattccta ttcaatttta aaattctttt 60 tattaataat gaaatcgctt ttattagtgg gagtcgtatt agcgaccttt tgttcaagta 120 gtatcgctga cgataaaaaa ggcccaaaag ttacagataa ggtatggttt gatattaata 180 ttgatggtaa atatgaagga agaattgaaa ttgggctgtt tggaaagaca gttccaaaga 240 ctgtcaagaa tttcattgaa ttagcaaaaa aacctcaagg agaaggatat aagggtagta 300 aatttcatag agtcattcgt gaatttatga tccaaggagg agattttaca aaaggagatg 360 gaactggagg tcgcagtatt tatggagctc gttttgaaga tgaaaacttc aaattgaaac 420 attatggtgc tggatggttg tctatggcaa atgctggaaa agataccaat ggatcacagt 480 tctttattac tgtgaaacaa actccttggc ttgatggtag acatgttgta tttggcaaaa 540 taattaaggg aatggacgta gtaagaaaaa ttgaaaatgt aaacactgat actcgagata 600 gaccgacaaa agatgttgta attgctgatt gtggtgcaga agtagtagca caacctttca 660 gtgtatcaaa ggaaagtgct actgattaaa catatttcta tttttatgat gatggaaact 720 aacc 724 // ID FN619844; SV 1; linear; mRNA; EST; INV; 698 BP. XX AC FN619844; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-698 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; c26d3b6df3a1b9dcb26557e6dbde172d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..698 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 698 BP; 222 A; 138 C; 118 G; 220 T; 0 other; tgcattagac aagaaaccgg atacgttttc tgcttaacgc gttgtccaat aaatttcgcg 60 ctttttgctt acactcccaa cgtaaaactt taacgtctaa catattatac acgtggtatg 120 tgttatatct gtgtgcaacg tgtaaaaaag attttaaaaa gtatttaaac ggaaattttc 180 taacacttga atattactag ctcgtaacat atgaaatatg ttttattaac tttacttagt 240 tgatataggt atgtccctgt ttttcgtttt taaacccaat ttaggaaaca ccaacgacca 300 acatgcggca tgttcgttac taaaatcttt ttcatcaata ttatcatacg tgattttggc 360 tacagaacga ccgattgcaa gcgttgcaag catgtacaca tacgttatat ccttgcaaat 420 aaaacaccaa cgaactttca aacacagttt ctacatgctt ggtacaatcc ggacgaagaa 480 tcggaaacat tgaaaccagc gtttcttacg ctgtgttacg actaataaga atcgtacaag 540 tatcgtcatt tgtcgaaacc aaatgcctgg atcaatttag atcatcggtg accggtatgg 600 cagtctgtgt tacttataaa taccaacgca ccgttttgaa actttcgtac gtcggcttaa 660 tcgttcgaaa aggtcgtcaa gacgagaaaa gttttacc 698 // ID FN619845; SV 1; linear; mRNA; EST; INV; 650 BP. XX AC FN619845; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-650 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b037f1a1649fabb4eaa52ad1fa7843f0. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..650 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 650 BP; 228 A; 112 C; 126 G; 183 T; 1 other; ctgacgcacg gtgattgttg tgacagaagt gaagaagaat atcatcgtat cgtcgtattt 60 ccagttcaga atcatcgtat gattcgatag gaaaaagatt ttgataaccc tagagaagaa 120 acgattatat taaagtgcac gggtctcgtg caagcgtgcg agaacgatga agtttcatta 180 caaggagaaa cactcctttg aaagaaggaa ggtcgagggt gaaaaaattc gacgaaaata 240 tcccgaaagg gttcccgtta tcgttgagaa ggctccaaaa gctaaagtta gtgatttgga 300 taaacaaaaa tatctagtac catctgattt gactgtggga caattttact ttttaatccg 360 taaacgaatt cacttacgtc cagaagatgc tctcttcttc tttgtgaaca acattattcc 420 tccaacaagt gctaccatgg gatctcttta tgccgaacat catgaagaag attttttcct 480 ctatatagca tacagcgatg agaatgtgta tggacactaa ttcacaaata taaagaagac 540 aacatccaaa caaaagagca gacagaaatt tgccatctac aataacgtag tccttataga 600 agcttttgaa agtgttaaca aaattatatc tttncattat tttgaatgaa 650 // ID FN619846; SV 1; linear; mRNA; EST; INV; 666 BP. XX AC FN619846; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-666 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 3671590b04c6ab4fd9747e6f79421ae0. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..666 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 666 BP; 185 A; 142 C; 139 G; 200 T; 0 other; gttatcgcag atttcgatgg ccggttgcgg tccaccgatc gttgggtatc tccgtcgagg 60 aaactctcgt ttctaggaaa cttgtgttgc gtaacgcgcg cgacgcgagt cggatgagct 120 acacgaccga acgattccac tgaaaacagc gaatcagctg ttctcctcga tttaactcct 180 ttgcttcgcg gttcgttccg cgttttaaaa ctctttttcc tcgaagatca aacgcgcgat 240 atttgcgtac cgtcgctcgt caaataatgg caaacatcca atttcagata caaatttcaa 300 gaagttgtat gcaaatcgaa attgcgaaag attctacgga tctgtggatt tttatcgaac 360 gtacgaaatc gacgacaaat tgtcgattag aatgttcagt gttgcaaaga atttacaatc 420 aaatgtacag tcttaaaata gaaaaatgtt aacaataagt cgatatcctt cgttgagaaa 480 tttaaacgtg atttcatcaa aattcaaatt tctctgattg ctgtgttcga tgcacagggc 540 tatttgcttt atttggtccg cgacttggaa accgcacaaa ctggctatca tgattttcac 600 gtaacaaagc cgcgttatga cggtttgaca ttaacagatg tcgatctttg tattccgcat 660 cgccgt 666 // ID FN619847; SV 1; linear; mRNA; EST; INV; 639 BP. XX AC FN619847; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-639 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 6079e96daeffb4eb56cbde985b5c4854. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..639 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 639 BP; 164 A; 128 C; 140 G; 206 T; 1 other; catttccttg tagaatcctt tcacgcatat tgcatttcca ctgcaacaat ttctcgcagg 60 tgctattgtc tctatctgca tatttcattt gtgactttta tcttgtctga cgagacaaat 120 tattcttcag aaccatgtta acgttatggt tgttggtcgt gttccttggt attatctatc 180 aaggctctac tttctggata aaatttgata atccatttgc gaggaacttg gaaaatgcga 240 tgcgtgtggt ttacgaatgg agatatattg actttgattt cggtagtgaa gaaagaaggt 300 aagctgccat tagttctgga gaatacaatt atacgaaagt caatcccata gacgtggatc 360 gatggcacaa tctaatattt gtcacggtga taagggatga aggcgtgcct tcgtctttga 420 acgtcatatc tgatagacgc ggacctggtg gccctcttct gacaccgtac cccaattgga 480 actggacaag cactaaaaat tgttctaaca ttataagcgt ttacagagtt tcgatcgaca 540 gatgcgacag actgtgggtc ttagacactg gtgttatcgg tgataatcac gtgtgtcctg 600 cccagctcgt tgtcttcgat ctaancacct ctgagctgc 639 // ID FN619848; SV 1; linear; mRNA; EST; INV; 695 BP. XX AC FN619848; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-695 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e06dd48b713ed42109e8312020e1a50e. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..695 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 695 BP; 222 A; 90 C; 144 G; 237 T; 2 other; gccattgtat catgtgcttg catgattgtg attcattgaa gatgaaattt gaaactattc 60 gaattttttt ataaatacat ataaaaaaaa gttcatattg ttttgttttc gtaatattca 120 tatatgtgtg cgcgcgcgtg tatgtaaaat ttatgtccta aatttgtaac aggaatatcg 180 taaaaaatat atttaggtta taatttgttg atgctgtctt tttttgaaat gtataaatcc 240 ttattggaag agagaggaag agggaggaga tatatgtata atatattaac ttattttgaa 300 cggagggcct cctattaggc gtgggtaatc ctcttcttga tatttcagcg attgtagata 360 ggaatttttt ggaaaaatat gatttaaaat cgaacgatgc aattcttgct gaagaaaaac 420 acaagccaat gtatgatgaa ttagttgaat tgtataacgc agattttatt gccggtggtt 480 ctgtacaaaa tactacgcga gtagcacaat ggttcctgga gaaaccacga gttgcaactt 540 acatgggttg cgttggaata gataagtatt ccaaaatttt ggaggataag gcacgtgcag 600 acggcttaaa tgtttgttat ncatatacaa agaaagaacc tactggtacc tgtgctgttc 660 ttattactgg caatgaacgt ccnttatgcg ccaat 695 // ID FN619849; SV 1; linear; mRNA; EST; INV; 654 BP. XX AC FN619849; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-654 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 9dd4a1ef1f3ab43d3510b9ab0878bfe8. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..654 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 654 BP; 205 A; 111 C; 151 G; 186 T; 1 other; acgtagagtt gtgaacgacg tgagacgaag cgaagacggt gaacgaagcg ataggatgtt 60 aatattagtc gaatcgttta cgaatttcga cgcgtcggat tacgtctaat ttttttttat 120 gtgacataca tttttacggg gtgtgaaaac acgagtttat ctgtgtcgaa gttcctgctg 180 cgaccttcga aggcgcgtgg tatcaccggc aatagtgtcc gacaatacag aatcattata 240 agattaacat ttactttatg aacgtgtttt cataattata tcaattttat atatatatgt 300 atcgcatatg taaatgtaca gtgagtggca tgcataaagt atttggaaac cgtacaattt 360 ttgagagctc cgttcatcga tatcttcctc tcgaacagcg tcgatgaaaa ctcaactata 420 aagacgccat gttagaagct gataataata attagaggcg cggacaaagt gactgtggtc 480 gatggaagcg atgacctgtg cgagtgtgtt ggtatggtga cattggtgca acagcatcga 540 caaacggacg aaaatcaata tctaaaagat tcaaatcaaa taagcaacgc ctccattgaa 600 aatcgcgata acgaaaattg tggaggtgag gcagtgcatt cctctngtga tact 654 // ID FN619850; SV 1; linear; mRNA; EST; INV; 668 BP. XX AC FN619850; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-668 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0072734f660738878bf72232cd6cb741. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..668 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 668 BP; 255 A; 80 C; 100 G; 233 T; 0 other; gaagtaagtt tcagaaacta atgttctaag atgttgcaaa ttgtaatttc cgagaatttc 60 tgtaatcgaa tccacgtttc gaagttgcaa tcaagagaaa tcaccatttt taacttaaaa 120 gagaataccg ttatatatag cgatttgtta aagaactgtc aacaaagctt gtatttctgt 180 attagaagtg caatgtagat aaattaattt tccgctctac tatcacgata tatgtatatg 240 taagcaaaca gacgaagatg attgtaatta aataactata gatataatgt agagttaata 300 gagcaccata ccagagtaaa aagaccttag tagtttgtta catacatgca tacatacagt 360 cttgaaacaa aaatgaattc tctgtcatgt ataataaata tatacatata ttgaaattaa 420 gtagtgagat gaaaataatt aaatgaatgc tgctgtaaat gtgtaatttc tgtaatgtct 480 ttattatgta tttttttttt cgagatatta cacttatgtt aacaaaacat aacgagaagt 540 ttttattatc agagaattgt ttcaatgatg attactttct aatttgtaca ttgtaataaa 600 tatacatcat attctaatac gtgagaagga aacttttcaa gaatatttac aagaacaaac 660 tatgttgt 668 // ID FN619851; SV 1; linear; mRNA; EST; INV; 671 BP. XX AC FN619851; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-671 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 45d70607027c97b6d6e50447cb83a44b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..671 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 671 BP; 230 A; 125 C; 145 G; 170 T; 1 other; ttttccggtc tatgcctcgt aaacagttct tcaaccatgg gtaagagaaa taatatgatc 60 cctaatgggc atttccataa agattggcaa agatttgtta aaacttggtt taaccaacca 120 gctagaaaat ttcgtcgtaa acaaagtcgc gtaaagaaag ctcgtgctgt tgcaccaaga 180 cctgtgaagc ttctgaggcc tattgttcat tgcccaacat tccgttacca cacaaaagtt 240 agggcaggca aaggttttac cttagaagaa ctgaaggcta gtggtttaaa taaaagattt 300 gctaggacta ttggaattgc tgtagatcct agaagacgta acaaatcagt tgagtctttg 360 caaacgaatg ctcaaagact gaaagaatat aaatccaaat tgattctctt cccattgaat 420 gaaaagagac ctaaagtagg tgatgctact gaagaagaaa tgaaagttgc cactcaagtt 480 aaaggtgaaa tcatgccagt gagacaccaa gcacctgcta aagctaaggc ccgtgtcatt 540 tctgaggaag aaagaaagtt ctctgcatat gtaactctcc gtaaagctag ggctgatgcc 600 agactagtag gtattcgcgc aaaacgtgtg aaagatgcag cangaaatcc agatgaagta 660 actaaagttg c 671 // ID FN619852; SV 1; linear; mRNA; EST; INV; 700 BP. XX AC FN619852; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F14 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-700 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e7a52d0ff1c7c77f8fbb13793a02db31. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..700 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F14" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 700 BP; 266 A; 98 C; 132 G; 204 T; 0 other; aagctatttt cgtgctatga tgtcaatatt caacgacatg gtttaacgat atagttatct 60 agtcatattt tttaattgta aataacctta tccttattaa atatttttcc gtaatatggc 120 caagactaaa acaatcagta aaggctgccc aaaatgtgaa cagcaggtac ctgtagcttg 180 caaagcttgt ccttgtggtc attcattttt taatgcaaga cgaaattcaa taaaaagtcc 240 tcctagtcct gataatggag taatgaaaac aagacgaacc aatcgaatca agcgtgagaa 300 accaaattat tatgatgctt tagaatacga taagcaatca aaaaagagtg caaaaatcag 360 aaaagcaaca gatgctgcta gtgacataga ctgtagacag ttaaacaaga agaagaaacg 420 aaaggtaaaa ggtaaaggga aaagtggaag tagtaatgga ataggagatg atgatgatga 480 aatggaagga atcaatggtc tgtcaccaga gaagcagctc acatgctctt tgatactaca 540 agaattaaat aataaaatga gagttgtagc ctggaaaccg acataatttt ttctattgtg 600 tcatattttg tactataaga aatactagtg tccattttta gtagaaagta atcattttat 660 atagatgttg gactatattt gaaatgctat ctattcattg 700 // ID FN619853; SV 1; linear; mRNA; EST; INV; 725 BP. XX AC FN619853; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-725 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 85d7b59e94f3a89479f9608e2e1e34e5. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..725 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 725 BP; 266 A; 113 C; 119 G; 226 T; 1 other; tctgctagca tcacagtcta ttgtatttta atacctaata taagactaaa cgaacaatca 60 tcgaatcata tcttttatat ttacttatga atcagatttt acacataaat tagtaaatat 120 atcagaaaag aaggtggtta aattagggtg gaagatgagt taacagctta tttatcgaaa 180 agaaatcttc atcaaattca ttggattatt ttatcgaaaa tacacaactt tcaacatgga 240 aacaataagt gatccttatc agagacatct tctcaacgat gattctatat ttaatgtcta 300 caatgaagaa tcaacaaact taacagtcgg tgttcccggt cctgacttgc tccaaaactg 360 tctagagatt ggaagaagaa aagaaagaag gaaagtatta tctgtttcaa tatggcatca 420 cagcagcagc agtaaaacac agatgctgta aaacgggaaa atttaatttt aacatgtgga 480 gctttgcatg gtttataact tttgttaaat accattcttt caccaaacgg gataattttt 540 gtagaagaag ttacttacat gacagcatta gacgctttca gacaatttcc attaatgaaa 600 atagtcgcag atgaatttga aaaatgattg tggaagaaaa acgcatggta attttttacg 660 aatgagcaaa aatattctgg gctatgttta cacgatcccn acttttcata tcgactggat 720 cactt 725 // ID FN619854; SV 1; linear; mRNA; EST; INV; 668 BP. XX AC FN619854; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F16 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-668 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ad41f32e5322e40ddce1a2681df76ecc. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..668 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F16" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 668 BP; 239 A; 109 C; 82 G; 237 T; 1 other; aaaatagatt agttttccac tacaaaatac aactgtttgc agacatttgc acagaacttg 60 tcgttaataa taattaagcc cgtatcatac acggataata attcgtatct ttttatctat 120 ttgatttatt tattcgagtt gttgaaggga gcaatgcata tgataattta aaacgatagt 180 acgtatttct aaacttctaa atttaaatta ctgcatctat aaaaagtaat acctacatgc 240 ccttatttta caacaacgag cccttttata ggttctacat ttcattttcg cagtatcaaa 300 tctttatagt catatgagag tatcgctaaa ggataaattt tatgtacttg aacttaatta 360 attaatacgt acataaacgt tataataaat agaacaatgg gaaaacactt tttgtagaca 420 ttgtaaattt gaaattaaaa agaatggaaa aagaaacaaa cgttgaatcc aactcattaa 480 acattaacaa tatataagac cacagaggat taatgaaata tttccccgtt tcctatctaa 540 cctactgttt caaaaaatcc aatcgttgcg tccaattttg attttcatta taatcacagt 600 aacataaaaa gaagtttgtt gcttttttct ccttcttctt ngtggctttt ttcttcccca 660 tttacacg 668 // ID FN619855; SV 1; linear; mRNA; EST; INV; 743 BP. XX AC FN619855; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-743 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; bd71e29a03376ef47ea22b267d4209ed. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..743 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 743 BP; 236 A; 133 C; 135 G; 239 T; 0 other; cgtcattgat accatacgtt ttaatatcac tgaatcatgg aagaattaat gtaccaacct 60 ggatggagtt attgatggaa gattaaaaac caagcaatca agcatccaag ctcggtagct 120 cgaaactcga aagttgtcgg cattacaggg accgtcaaac agaatgaatt tcgagcaaat 180 tgttaaatca tcaatggttc gcctatgtca aatcagtaag gaagtttgtc ctataaactc 240 gggcatatga ttcaaggcat catttcattt gttgcccgca cctttgcgtc gcgtcacttg 300 tgtttcgtgg tcgctgcgat caagcgtgta tagagtacat acttaaaacc ttgtatgtat 360 gtatattcag ttgaacggtt gaacccagtg cagtgtctgg cgactggtcg atagtgtgta 420 acagctttta cttacaatta acagtattac ctcagccagc agcatcaaaa gttaaataga 480 aaattttgtt ttcgctgacc ttgaaatact tagattgtaa gtggttgagt ttgtctatta 540 cttacaagta aacaaaatgt aacattctat ttaagaaaac aaaatcgacc tttttatctc 600 attaaccgtt tgcaacgcat atatttttac tatatatcgg cttatacaaa gttatagaac 660 ttctatagga aatcttcttt tcaaatttta ttttcacatg gaaatattcc agaatgataa 720 gtagacgata cactctgtat gta 743 // ID FN619856; SV 1; linear; mRNA; EST; INV; 666 BP. XX AC FN619856; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-666 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8b6d87e96a3ec58d48b93d462d6a7dda. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..666 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 666 BP; 274 A; 79 C; 85 G; 228 T; 0 other; ttcagttagt gatctccgtg ttgtaaaact ggattttact tattaaaaat tatgagaaga 60 aaataggtta aaaatttgac gtattccaaa ataagtttaa ccttaatatt ttttaaagaa 120 tattaaaata tatcgtgcct ttattgttat gttctgttca actgtgattg cttttcgagg 180 ttagaaaatg acaacagact ccatacatta aagtgaaaaa tataccaaaa cgttttgtaa 240 ttttagtaaa attgagatta attgaaataa cataaaaatg ttttcatata ttcgtcaaac 300 atcagtgtta cgtacaatta ttagaaatgt aaacataaat actactgatc agaatattga 360 taaatattgg caacatatac tgaataattg taaaaatgca ttaacacaac ctagtactat 420 atcaaaagaa acatgggaaa atgtaagaaa acatatgata gaatcacaat gtgatccatc 480 tactcttgat tttattattc taaacatgtt tcaaagaatg aaatatccag atgctggaat 540 atcttattat aaatttttag cagataataa ttataaacct gaaattcctg ttattactaa 600 atatttacag ttgtatgaca taaaaaatga tccaatatca gaatcagata aagaacatat 660 tttaag 666 // ID FN619857; SV 1; linear; mRNA; EST; INV; 657 BP. XX AC FN619857; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-657 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 5eac0eae2e93a594a786cf4e71bd2ae2. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..657 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 657 BP; 249 A; 58 C; 95 G; 255 T; 0 other; gatctaaatt tggctctctg tttctttatt aatcgagata aagagactca tttaaccctt 60 aacaagaaat gaagtgagtt gtaaactcaa tgattcttat cgcatttctg cagatatatt 120 tagttatttg ttattgataa gatttatact taagtatttt ttctttattt ttcttcctag 180 aaaattgatt gcactttagc atgtttaaat ttatataaat aagtgagttt aaatatttta 240 tgtaaattaa atattaaaag gatgttacat ttaaaaattt tatgttacag tatcgtcatt 300 aattttattg ttattattat tatttttctt tttttttttg attgaatgat tcgattaaga 360 tatatacata taaatagcaa taaaaggaag gagcaagttt acttttaaaa agtagacatt 420 ttgaataaaa agtacttttg ttaagggtta agaaaaacat aagaactgtg ctgtgaatga 480 aaaattcagc catattaaag atctaatctt tgttatgtcc aaaaaaaaaa aaaacgaaag 540 gaacaaatgt ttggatattt agaaaagcat ttattagtaa gttgagattt cataaaaatt 600 tcttaacgaa aaaggtgtga aaggtaatct agtagttatt gaataattac aagagcg 657 // ID FN619858; SV 1; linear; mRNA; EST; INV; 718 BP. XX AC FN619858; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-718 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 75650bffc951f7c8e2dcb43b7d20d826. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..718 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 718 BP; 203 A; 119 C; 173 G; 223 T; 0 other; gaggccatat tgtacagtta aattgaaaga acgaaaataa ttatatcatc gtatagtcgt 60 gcttgtttcg tttcatattt ttttctaccg tacatatcca cctaggtgtt gtacgtacgc 120 cgtgagtaga taaacagtgc gcaaagtcta tttggttgct tcgacaattt catttttgta 180 aataactaca agttgtagta gtgtgttccg atatctaggt atcgtccgtc agtctactaa 240 aggggtttat tgctccagac gagatgggca ctcgggatga cgaatatgat tatttattta 300 aagttgttct tattggtgat tctggtgtag gaaaaagtaa tcttttgtca agatttacaa 360 gaaatgaatt caatttggaa tcaaagtcga ctataggagt tgaatttgct acgcgtagta 420 tacaagtaga tgggaaaact attaaagctc agatttggga cacagcagga caagagcgtt 480 atcgcgctat tacatctgcg tactatcgtg gtgcggttgg agctctgctg gtgtacgata 540 tcgcgaagca tttgacgtac gaaaatgtgg agagatggtt gcgagaattg cgggaccatg 600 ctgatcaaaa catcgttatt atgttggtgg gaaaaaatct gacttaggca tctacgtgcg 660 gttccaccga cgaagcgaag cgtttcgcga gagaacgcct ctcttttatg aactctgc 718 // ID FN619859; SV 1; linear; mRNA; EST; INV; 715 BP. XX AC FN619859; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-715 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b52968ecf1ed9db6fdcc5a415bd4da94. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..715 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 715 BP; 235 A; 128 C; 165 G; 187 T; 0 other; aagctgcacg cgataagacc gttcgtagga attccagact ggattactaa ttcaacgaaa 60 ttggataaat tttacgaggg gaaaaatgtt gtgcctgggc gattgtttga tacatttttg 120 atgctaactg acgcaacagt caagaaaagt ttaaatagtt tgcgtgagaa accggacaaa 180 aatcgatgga tatcgactgg gacaacagta aatgcgtttt atagcgcgat attaaactca 240 gtcacatttc cagctggtat tttgcatcct ccgctttacg ggaatggttt acagtctatc 300 aattacggtg ctatgggcgc aatcatgggt catgaactaa ctcatggatt cgatgatcaa 360 ggtcgtagat atgacgaaaa cgggaatctt cagcagtggt ggagcaacga gacgttacaa 420 cgttaccacg agaaagttga gtgtataatc aaacagtacg gtaactatca tctgccagag 480 ctaggcaata ataataattt cacggtaaat ggcgtgaata cacaaggaga gaatatagct 540 gataacggtg gtatacgaga ggcctacaaa gcgtatcaac gatttcgaac aagaaatact 600 aatcgactag ctctacccgg cctcgctaat tatagccaag aacaattatt ttttataggt 660 ttcgcacaaa tctggtgcgg tagttatacg aatggggcgc taaaatctaa attga 715 // ID FN619860; SV 1; linear; mRNA; EST; INV; 735 BP. XX AC FN619860; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-735 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 156f32a40a7f7ede3e655a6bbd77c598. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..735 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 735 BP; 224 A; 143 C; 187 G; 181 T; 0 other; gtcatttata cccaggcttc tgtgcgcgat tgtttcgtct tcgttcgtcg ccatcattgc 60 gaagacgaaa gaggttggtt tcattcattt tcgtgtggca gtgtcattat acgaaatcca 120 gcacaaatga agatggttga taaaatagac atgagtctcg acgatattat caagcaaaat 180 aaaggaacaa gaccacgcgg cggcagtagc agacgtggaa gaggtgccgg taattcacct 240 cgtagaggag cacggagaac gcaaagagtt ggtggtggcg tcatgcgggg acgcaatcgt 300 ggtggcatta caagaaattc tcttccatac acaaggggcg atgtaaacag tgcttggaaa 360 cacgacatgt ttgatggagt aaaaaaggtt ggccgaagtt caataggtag cgcagggaca 420 accaagctcc ttgtatcgaa tctagatttt ggagtatccg attcagacat tcaggaatta 480 tttagtgaat ttggcccctt aaagtcagca gcagtacatt acgatagatc aggccgatca 540 ttaggatctg cagatgttat atttgaaaga agagcagatg ctattaaagc aatgaaacaa 600 tataatggcg tacccttaga tggtcgtgaa atgaacatac aagttgctac ctctgaaata 660 ccagtcacgt ctatccgaag aggacccgag actagcggca gtattttaca cagagacctc 720 agtcccgctt taggg 735 // ID FN619861; SV 1; linear; mRNA; EST; INV; 613 BP. XX AC FN619861; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F23 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-613 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; d5c5d551d09d612ce95fe5d2fcca7841. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..613 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F23" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 613 BP; 205 A; 82 C; 116 G; 210 T; 0 other; ctctacatta tagatgggat ttatcacttt gcaagttgca aagtgtatat aagttgcaga 60 attggacgtt tcaacacaga gttggatcat aaagcgtttt gaacagtcgt taaaatttat 120 ttaatttttt atttctgttt aatttagtta caatggattt tccaacagat gaattattaa 180 aagaagtttt acagtatcca gctgtacaaa gtatgaaggt taccgtaagt tcttgtggaa 240 aatatgcagt aataatcggt ggatcagcat ttcttggtgg attaattggt ggaccaatcg 300 gacttgcagt ggttggtaca gttgttagtg ctctatcagc atttttcgga tatcatgaaa 360 tcaaatctgt tccatatatc attttgtacg aaactactcc agaacaaaga gaaaagctag 420 cactatcaat agttaagtac cttaatttaa aaaatatttg gtctcttaga gatctcttat 480 actataataa ggaagaattg gcattaggga taacatctat aatagtaaaa tttgtaactc 540 atgacatgcg ttattcaatg agaaactgat tgtggattag aaagagagat cgccctgtat 600 agaatttatt gac 613 // ID FN619862; SV 1; linear; mRNA; EST; INV; 682 BP. XX AC FN619862; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051F24 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-682 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; d5104f50b1fa882041fedab85edfe823. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..682 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051F24" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 682 BP; 206 A; 88 C; 115 G; 272 T; 1 other; tggtatggct cttgcttcag taccagtgat gtcaactttg aataggagag gtgttctact 60 tactattata tatttgtgct taggatgttt gatagtttta tgtatttcca ttttatttat 120 tcatttgtca tcacatatgc caataatatg tacaatagcc gttctggctt acgtgatatt 180 ttatggaatt ggtcttggac caataccata cttcattgga tctgaattat ttgacgttgg 240 tcctagacca acagctatgg catttggtag tgtttttaat tggggtggaa attttatagt 300 aggcatgact ttcccaacaa tagaaagtat tatgggacca tatacttttt taatatttgc 360 cgtatttctt ttattgttag gacaaattgt taggatatac ttacctgaga ctagaggaaa 420 gaatacgatg gatatagcag cattaataag tcaaggattt aactctagac caaattccat 480 ccgtcatact taaatttaaa aactggtact ttatatggtc tagtgaatag ataggtttac 540 aattttttcg ttgtaaggtt tatgtaaata tttaattaat aagggttact ttttacgatt 600 tagataagaa attnattata cttttatttt ttatttttgt aatactacat ttatctaaac 660 atctgtatct aaataattac aa 682 // ID FN619863; SV 1; linear; mRNA; EST; INV; 617 BP. XX AC FN619863; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-617 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 113016da73b3f92ae5fa5b0b12444567. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..617 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 617 BP; 199 A; 65 C; 110 G; 243 T; 0 other; cgtataatta gatgtgcttt gcaaataata taatagtgta ttctattcga ataatgtgta 60 tttatatgat ttttgtgttt ccggaatttt tggtataaaa tgctgtattg tgtgcgcgta 120 atataattcg caattgttta aaatgttgga atgaaagctg ctagttgatt attatgcgta 180 tattaacctc aacagtcgtc gatattttat ttctgtatgc gattaattgg tcataaatag 240 cataaagtga gtgttttgaa aggatctatt atttagattt atttgtagta agtgcatctg 300 ataataaatt gtactttttg tgcataaaaa tattccatga aattatcata acctatattc 360 tatatactta cgctatttag ttttcttgat attatgaagt ttataataat ataggatgaa 420 aatggaacat ttttataatt tttatgtcgt ccaggctgct caattatgaa tcctgatatt 480 caggaaaaga ttttcaagtt tgaaactttt gtcaatgatg tgttgaagga ggatcttgct 540 aaattagagc agaaacttga tactaagaat ggagaaattg cagagtttct tcagttaaag 600 ggtatgataa ctaccct 617 // ID FN619864; SV 1; linear; mRNA; EST; INV; 692 BP. XX AC FN619864; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-692 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 823d1b6d642c78db5a6bb1d5503cc60b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..692 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 692 BP; 271 A; 108 C; 125 G; 188 T; 0 other; ggccgtcaag cgaaaatcga gaaatacgaa aaacgaacga gagtgtaacg atcgaatgtg 60 aatacgagtc agcggttttt tttacgaatt aatcacggga taacgaacga aatgcaaaac 120 gatatgagaa tgtatacgtt aaagccgatt gtttctttgc cacaaatgtt gcatcatagg 180 aaaacgatgt acgaaatgcg aaatattcat gaagaacaat caacagacga ccatatggaa 240 gccgagaaca agatcgtgcc tgatatcact gagcaggtta tcagcttttt aaagagtcta 300 ctaccagaat ataatgcatt agaagcacga caagaaaaga tactggcaca acttgcagaa 360 cttaagaaac aagtttcgac actttctggg tttttaaaac aaacgaaaca aactacagta 420 gttgataaaa gttctacagt taataagagc caggagccaa ttaatgtgaa tcttatcatt 480 aatgtgaatc ctaaaagaca accttatttt atatcagcat tgcaaaaatt gtggaaagat 540 actgatatta aagtacaaac ttatgttcat tcaagtatta ataaagaagt gtctgttaat 600 tatgaatcaa ctacaagttc ttccaaaaat aatattatta atttatcatt gatctggaaa 660 aaatgtaatg atctggaact tatatctggt ct 692 // ID FN619865; SV 1; linear; mRNA; EST; INV; 706 BP. XX AC FN619865; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G03 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-706 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0a3319785464ca01c537d8f44f6f3b98. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..706 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G03" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 706 BP; 206 A; 168 C; 152 G; 180 T; 0 other; tgaatctcag aagggtatga atttcgcgac tgtagctggt ggtggtggag tgtccgttaa 60 catttcgggt tcatcctcgt ctaaattcat cgacagcata ccccctcagg tggatgcttc 120 aaaagctcct ggatatcgag ggacagctat gtgctcccct gtttcaagta aatcaaataa 180 tacaactaat acgaacgcga ctagcatagg aagcggaggc gtatcatcaa atactggtca 240 taatcctatt caaccaccgc aatttcagtc gtctggaaat tataatgaac atcctcttcc 300 aagtaaacca ccaggtagtt tggctgtagc acggccggta attcctcagc aaaatataga 360 aatgggagca gctatgggag ctcagtttag tagaccagtg tttcaagggg aattgacagg 420 cactcgcaat gcgacaaccc atcagccaca cgtgattgcc tctacaccac aactgacgtt 480 ggacgttggt ttgtttaaag gaaacaacat tggctatgag catccaaacg ttaattcaag 540 tttattaaag atggtaccaa acgaagggca aggtcatccg cttttacctt tccacccgca 600 tatgcagaat ttcgcgcaaa caatagcgcc atctgcttcg gtgaacacta cagtcagtat 660 gtccagatta aaccccagag cacctgactt ttctagctcg ttacat 706 // ID FN619866; SV 1; linear; mRNA; EST; INV; 682 BP. XX AC FN619866; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-682 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; afacd85b7424f8ed967f5431973aa3ba. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..682 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 682 BP; 226 A; 150 C; 144 G; 161 T; 1 other; aaattttgat ccgatcgtgt gttgtccaaa aacacgccta cgactacttc tccaaataca 60 atcacaaaca caattaaaaa cacaaccccg actacagcag atcgaggatc agtcgttact 120 aaaggaacca aggccaatgc aatgtgtgaa aaatacgcca aatccgtgta tcagctcgta 180 ccttcgccag ctttaataat tgacagacca ctaatcaaca tatccgtatg cgcgattaaa 240 actcgtaagc tgatcatcgg tggtacaaag gctgaaccaa aggaatttcc acatatggct 300 gcgattggac atgataccga aaatggtatc gattggcaat gcggaggcac gctaatttcg 360 gaaagattcg ttctaaccgc tgcacattgt acctacagta tcaacggagg tagagcacgc 420 tgggtacgat taggtgactt aaacctcgag aatacggatg atgacgcgag accgcaaaat 480 taccttatta ttgagcggat aaaacataat aagtaccaga gtcgattcgc atatcatgat 540 atcgcgctac taaaattaga aaaagacgtg gaatttaacg cgtggataag acccagttgc 600 ttaccatact ctttgccgga tattggtacc gatggcaaag caactgcatc cggatgggga 660 acagtgattt ttttgangac ca 682 // ID FN619867; SV 1; linear; mRNA; EST; INV; 700 BP. XX AC FN619867; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-700 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 62afd23dffefd6136fd05aef850dac2e. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..700 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 700 BP; 258 A; 103 C; 116 G; 222 T; 1 other; acgtttgtta gtatcgcatc gttgatctcc gccgaatttc gcagcggacg cgttggacaa 60 aatgcgcgag aagattacga ctcgtaatat cgcgtaatca tgatatatcc tgaccggtgt 120 tcgagaaccg aaataatgcg aggtgaacaa gtatgaaaga ttaaacatgt cccatggctg 180 gaggcagggg caacaacagt caaagtggaa atttagacag aatacaccaa tggctatgtt 240 gccatcaact ttatgcttct gtcaagcctg aacctccttc cagtgaaaca tttaaaaaac 300 atcaacatat agaaaagagc cgaattgttg caaaaagtct taatgatctg caagataata 360 ttttgaggta aataaatagt ttcctatcta attcacagat tattaattaa gacaaaaaat 420 aatttaacga ataattaact taaaataatg ctaatattat ttgttaatgt tttcatataa 480 tacatgagat aacaaatata aatatcttca taaaaatcct tgcatgatat aattgagcag 540 tttcagtgaa ttactttgtg ctatatattt taatttatgt aattgatatg tataatatcg 600 agtattggaa caactagtaa attgtacaaa attatggaac atttgaaaat tctaattaga 660 attactttta gncagattat tttatatatc ataattgtgc 700 // ID FN619868; SV 1; linear; mRNA; EST; INV; 688 BP. XX AC FN619868; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-688 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; d7f9d584b0c59994b2fa3bf44120683a. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..688 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 688 BP; 236 A; 108 C; 122 G; 222 T; 0 other; ttaataatgc gaagagactt tgttacatta tatgtcatta attgtattaa aatttgactg 60 agatattatt tatttcagta caatgacatc gatattctgt attaccttca ttaaaggcaa 120 ttccatcgga tatttaactc gattatgtga ggcaccaaag aattttaata ttgtgacaca 180 agtggcattt atgaaaacat tgtcaaataa accctttaca cctaaaccag ctccatttcc 240 ttattggaaa aaattatgta atgaaataaa tatgactttt gatccaacaa ctctcagatt 300 tgatgaaaat accaaagtaa tagtcgtaga tggccctcct gcatcaggaa aaagtaaact 360 ttgcgaacaa atagcaaagg aatttggttt attatatatg ccaccaccag tatttgatga 420 aatgtatata aattattatg gatttgacgt acgcagtcta aatccaaaac tgaaagatga 480 gtggcgtatg cgtgatctga cagatttcct aagagatcct gataacaggg gaacagcccg 540 tatccaatac ggaatattta tgatgagaat ggaacaatac ttaaatgcgt tacttcatgt 600 tttggcaact ggacagggag tggttcttaa tcgttgcatc tttacagaag ctggttatat 660 gcatgctatg tataattctg ggtatctt 688 // ID FN619869; SV 1; linear; mRNA; EST; INV; 715 BP. XX AC FN619869; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-715 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; caddf9b1ae8cffd09fc7d04380887ef4. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..715 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 715 BP; 243 A; 94 C; 133 G; 245 T; 0 other; agaatgtttt ctcagcttaa gttgtgttaa tttagtgtta tattgtctct ttattacgac 60 tcttgcatta atttaatttc aaatggcgta taatattggt tcttttataa gcactcctgg 120 cttttgttct gattgtgggt ccattctacc gttgttaggt gatagaggaa atgtaaaatg 180 ttatgcttgt aaaagggttt ggggtcccga agcttttgga gatatggcga tgtcgtacac 240 tattgagttt aataaaaaga atgtctatgg ttcatcaaaa gaaaagaatg atgcaatgga 300 agaagcagaa ggcccaattg ttgaaagaaa atgtcctcaa tgtcaaaatg ataaaatgtc 360 atatgctaca ttgcaattga gatcagcgga tgaggggcag acagtatttt acacatgtac 420 caagtgcaaa ttcaaagaga cagagaattc ataacagaaa attttattat acatttcaat 480 ttttacattc aacactgttt aattagttga ttggtaaatg attatcaaaa cataaaacac 540 tctgtgttaa taaaacatag agctttattt atgatgcact caacaaatat caatgaatcg 600 gattcataat aatagtaagg agagaaatca cataataatt ttcgtaataa cgctgagtta 660 ttgttatatt tttcccctaa tacgcatggg tggaaattcg taatatgtat ctttt 715 // ID FN619870; SV 1; linear; mRNA; EST; INV; 693 BP. XX AC FN619870; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-693 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; ce979b8f933b5df22ad5306229b8fcc2. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..693 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 693 BP; 240 A; 93 C; 108 G; 252 T; 0 other; ttgtatatag tatagacaat taatttcata catgatgaaa tttatgatca atttaataca 60 cgcgattctt atttaaagct gttcatttat aattgtagga taaaatataa gtactttttc 120 tcatttgcat cgctgacggg acacgttaaa atatcgaccc tgttactaaa gtatattatg 180 atgtgagaac atctttctat agacgaatgt ctagttacgt gtgttcttcg atacaggtca 240 aacataagtt gtctttaacg tgtcttatta agaagtgaac ggatgatgga cgaaatttta 300 aaacaaggtt cagatatagg aacattttgt gacactataa agtgcctgtc ttgtcatgtt 360 tagagacgtg aaaaactcgc gctagattcc ttatgatctt gaaccatcaa cagaataatt 420 tcatattttg tttaatacag atacagattg agaaaaaata tataaattta tgttgacaat 480 gattatataa gacaaggttt ctacatatgg ttgtactaca ttaaacgtaa taattaatgt 540 ctttcctaat tatattatat cttaaacata tattactgag aatgaaacct tggatacttt 600 tagtttaagg aatacaaact ttcgaatttg ttacttttat tctttgcatc ttcgttacgt 660 ataggagttt atcgtaaact aaagctaaag aac 693 // ID FN619871; SV 1; linear; mRNA; EST; INV; 725 BP. XX AC FN619871; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-725 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 094cfc078de91dd4e6d2ab5a8cda7d9b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..725 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 725 BP; 256 A; 113 C; 142 G; 214 T; 0 other; atcgcgtcag cgtataagaa gatagtaata tttccagtag tattgaaaag tttattttaa 60 gacatcatat tcaggcaaat atcaactttt attcgtttaa atacttgaca tataatagga 120 gtcaatatgt cgattaacat gaaacgcaca taatgtatgt atactgatgt atatacctcc 180 tctttcttga ggttctaatt ggagcatgca ccagccttta tggactacag ttgcagcgtt 240 tctgtttata aatgctgaaa aatgccacca aaacaaagga aaatagctat tatgggttat 300 agatccgtag gcaagtcgtc actgagcata cagttcgtcg agggacagtt tgtggattca 360 tatgacccta ccatagaaaa tacatttaca aaaagtacac gagtaaatag tcaagattat 420 gaagttaagt tggtagacac agcaggtcaa gatgaataca gtatatttcc tacacagtat 480 tctatggata tccatggtta tgtcctggta tatagcataa caagtgctaa gagttttgaa 540 gttgtacaaa ttatttatga caagttattg gatataacag gaaaagtcca tgtcccaatt 600 gtattagtag gaaataaaac ggatttatat gtagatcgaa tgataacaac agaacaaggg 660 gcagcgatag cagaatcttg gcatgctgct ttcctagaaa acagtgcaaa caaaacgagt 720 ctgtg 725 // ID FN619872; SV 1; linear; mRNA; EST; INV; 694 BP. XX AC FN619872; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-694 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 4628b6fc0ef5c5972ef5444ecb4239f2. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..694 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 694 BP; 222 A; 130 C; 156 G; 184 T; 2 other; caacacttcg tcagaacggc gactcttgag ccagtctgaa tgaaaatttg gtagcgactg 60 gtctcatgct agcaaaaatg ctacattgca cgacgttgtg ttatctggct gcagctgcat 120 tgctcgctgt atttttgttc ttttcggcgg tctacttttt cattcagtac acgttcggag 180 tctgcaagag taagaacaga atggatggaa agactgtgat aatcactgga tgcacatccg 240 gcattggaaa agaaactgca aaagatatcg cgagaagagg agcgagattg gtcatggcct 300 gtcgaaatat ggagtcagcg aacaaattaa aagaggaatt aataaaggaa tcaggcaacg 360 agaacatcgt tactcgagag ttaaacttgt cgtcactgac ttcgataaga aaatttgcgc 420 aggaaataaa ccgtgaagag tctaggttgg atgtattaat tcataacgca ggaactgcag 480 acttattcac aaaaaaagtg acggaagacg gtctggaaat gactatggga acaaatcact 540 atggtccatt cttacttacg catcttttga tcgacttact aaaacaatcg aaaccaagtc 600 gaataatcgt cgttgcatca ngactgtatt acttagcgag attaaattta aacatgtaat 660 cccacgacga ccttgctgnc tattgtacta tgtt 694 // ID FN619873; SV 1; linear; mRNA; EST; INV; 650 BP. XX AC FN619873; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-650 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 4ea5fd0483136899dc228a42293d6c67. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..650 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 650 BP; 207 A; 98 C; 147 G; 198 T; 0 other; gattatggca gacaacaacg tgtttgcatc attggatcag gaaattgggg ttcagctatc 60 gctaagatag ttggagcaaa tgccgccaaa cttaataatt ttgaggatcg tgttacgatg 120 tacgtatatg aggaaactat caatggaaaa aaactaactg aaattattaa tgagacgcat 180 gaaaacgtaa agtaccttcc aggacataaa ctcccgccta atgtgattgc agttcctgat 240 gtagtgaaag ctgccgagga tgcagatatc ctaatttttg taataccaca tcaatttatc 300 aataaaatat gtagtacgtt attagggaaa attaaaccta cagctgttgg tctttctttg 360 gtgaagggtt ttgataagaa ggaggggggt ggcattgaac ttatttcaca tataattact 420 aaacaacttc atattcctgt ttcggtttta atgggagcaa atctagcttc tgaagttgcc 480 gaggaaatgt tttgtgaaac tactatcggt tgcaaggaca aaaatatggc ttctatacta 540 agggatataa tacaaacgcc atattttaga gttgtagtag ctgaagatgt tgattctgtt 600 gaatgttgtg gggcattgaa gaatatagta gcctgcgccg ctggattcgt 650 // ID FN619874; SV 1; linear; mRNA; EST; INV; 702 BP. XX AC FN619874; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-702 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a1b4ee455413c8f288593e164f69cba6. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..702 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 702 BP; 217 A; 126 C; 143 G; 216 T; 0 other; cgttcaaata caacatgtac aggatatcgc gaatttgcga gagacagtag atacagtgac 60 agagtgtggt atttatagca attgttataa aatgaaacgg catgttccac ctgatgcaat 120 accttagcgg actctttttg ctttttctct cgataccgct gagcaatggt gttgattaca 180 cgtatgaaat cgactgcagt aacctacgaa tgggacaata tatctgtcct catcccgatt 240 atgatttcat agatccgaaa acgcaacaac ccaaaggttg cacgaaggaa aataaagcca 300 aagtgttatg tctggctgcg gatggcctca tttgtacaga aacaaaaaat aatacattca 360 agaaagatgt tccttgtaaa tggacaaacg gatattcatt cgagacatca ttgttactct 420 ctatatttct tggaatgttc ggggcagata gattttatct tggatatcca gcattgggct 480 ttcttaagtt aagtactttg ggattcctct ttcttggcca atttgctgat ataatactta 540 ttgcaacaca aattgtggga cctgcggatg gttcgcatta tgtgatacca tattatggtg 600 caggaattca tattgtgaca agtaataact ttacgtacag agtgccacaa tacgaatggt 660 gaatgatttt ttctcataca gaagataacg attgatcagt ct 702 // ID FN619875; SV 1; linear; mRNA; EST; INV; 519 BP. XX AC FN619875; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-519 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; dc0fb6410294f526789ff06b68793d55. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..519 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 519 BP; 154 A; 93 C; 119 G; 153 T; 0 other; atagaaaagt aatcatggtg tctttagaaa ctattgcggg tatagcgata aaagtgctta 60 aactggttat taatctcata atcctgatcc tgtatcgaac aggataccaa ggggaattcc 120 tgggtgttgg aggaacatgg aacttgaacg aagacaaaaa tccggacgca gaaatcgttg 180 cgtctggagt tttagtggga tttttcatat acaccagcgt tgttctaatt acttattgct 240 ttggcaactc gtcgcacaaa aagacacttg ttgaaattat aatgaacttc gttggaacct 300 tcatgttcat tgctgtaggt ggcacagcac tccattattg gcacggatat cgatcagaaa 360 ataaattcga tacgattgtc caggaacgac aggtcggcct agctgttgga tcgttgtgcg 420 ttatcgaggg tgcagcgtat ctcgtcgatc taatattaag cgttctacat tttgcgaaaa 480 gccacgatga atatttagaa taaagtcacg tttcacgta 519 // ID FN619876; SV 1; linear; mRNA; EST; INV; 116 BP. XX AC FN619876; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G15 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-116 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 8bf75897705fe84773697abb702b6157. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..116 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G15" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 116 BP; 26 A; 31 C; 20 G; 39 T; 0 other; gcattataca cgagcaacat ggctcgaagt ttgatctctg ctctcgctgt atttttggac 60 atcatcagat gatttgccgc tcatcgagtc atcactcatc tgatcatctc tcatct 116 // ID FN619877; SV 1; linear; mRNA; EST; INV; 677 BP. XX AC FN619877; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-677 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 2e59e9d93485a8b57c73f04fead282ee. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..677 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 677 BP; 176 A; 137 C; 167 G; 197 T; 0 other; cgctcgtaat catcctcgtg gcaatggagt catcaatgga gtcgttctct ttttcgttca 60 agttgttagt gatcggcttg atcgtcgtta gtctggccaa gttgatcagt ttgtttcatc 120 accagtacag ttactggaaa aagagacacg ttcctcatgt gggggcgatg cctgtacttg 180 gatccgcgtg gaaactgttc acccgttgcg tgtcccttcc cgattattgc acgttcgtct 240 acaattatta tccgaacgca aggtacgtcg gtatgatgga tttctctacg ccagttgtgg 300 taattcggga tccagagatg atcagggaga tagctgtgaa gaattttgag cattttcccg 360 atcatcagag tttcatcaac gaaaagatag atccgatctt cgggaaaaac gtgttctcct 420 tgagaggtga tcgatggaga gagatgagga acacactgag tccatccttc acagctagca 480 agatgcgatt catgttcgag ctggtatcta aatgttctga ggaattcgtc gaccatttat 540 ataatcatcc agaattgtcg tgttcgatag aggcgaaaga tgcgttcact aggtatacca 600 acgacgtaat tgccactgtg gctttcggaa taagtgtgaa ttcgtttgaa aaccggaaca 660 cgagttctat aaaaaag 677 // ID FN619878; SV 1; linear; mRNA; EST; INV; 677 BP. XX AC FN619878; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-677 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; d92320ac64e3b65f957e72841cd92d80. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..677 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 677 BP; 208 A; 141 C; 165 G; 163 T; 0 other; catgatggaa cacggctaaa cgataacatg ttgaccaatt gggataatat tccgtctttg 60 aaactcggcg atttcacgct cgagttcgaa atatcaccac ctggtccgga gttgcaggaa 120 gtagcgaaaa aggaacttcg tgagacgcca gaaattcaaa atgaatcgat cgctcaactt 180 aaggaattac tgaaagccga aaccgacttg aagtgcccgc tggacaacga tgcctggctg 240 attcggtttc tcaggccgtg caagtactat ccagaatcag cattgaaact ggtgaagaat 300 tactacagtt ttaaaataaa gcacgcagac atgtacgata atctgaaacc cagccgagag 360 aagaacatat tcgagcacaa tattttgacc gtgctaccga accgagatca atgcggacga 420 agggtattaa tcatcgagct taggaaaaaa tggaagcaca acaagtgctc tttggacgag 480 gtgttcaaag gttgcgttct gtatgtggag gctgctatgt tggagccaaa tacgcaaatc 540 gctggtgccg tagttgtttt cgatatggat ggacttactc ttcagcaggc ttggcaattc 600 actccagcga tcgcgaaaag gatagtcgat tggctgcagg atgcggtacc tctccgaatc 660 aagatataca tattatt 677 // ID FN619879; SV 1; linear; mRNA; EST; INV; 719 BP. XX AC FN619879; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-719 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 7089f13931badfdca8e2ab17099c6971. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..719 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 719 BP; 234 A; 145 C; 147 G; 192 T; 1 other; ccgacgtcga cagcatggac taccggttca tagtagtatg tataagagtc taagaacaaa 60 tcttcctcgg gaaataatgc aaatacctga ttttccaatg acacatgacg aagggccatc 120 atttgttcat catagcgtaa ttcgagaata cctttcggat tatgtaaaac attttaattt 180 atatccacat ataaagttaa atacactggt aaagcacgtg gaaccagaaa ctttgagaaa 240 cggtcaaaca atctggatga tcacgtacga ggatttggaa aacaaagtag aaacaactaa 300 aacgttcgac gccgtggtct tgtgtaatgg tcactatact gtaggccata ttccacacat 360 tccgggtatt gaaagtttcc ccggcgaatc tatacatagc catcagtaca gggtaccgga 420 agtgtactct aggaaaagag tttgcatcct tggtgcctct tggtctggta ttgatatcgc 480 catagaagtc tcgcaatacg ccgaaaaggt atatttgagc cacaatctac cggaaccagt 540 tgattcgaga atgtcgaaaa acgtagaaca gaggcctgga attcaatcga ttcaaggaaa 600 catattcatc ttccacgatg gttccacggt tgaagtagac aacttcatat attgcaccgg 660 taaaatgact tgatctcctt cgtacatata agtgatcnga ggtataaatt tacgtatcc 719 // ID FN619880; SV 1; linear; mRNA; EST; INV; 706 BP. XX AC FN619880; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-706 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; cd004c994e9749c08c31569a03e321f5. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..706 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 706 BP; 286 A; 69 C; 126 G; 224 T; 1 other; tcgatcgtat aacgagaacg cgtagtagaa agtagaacat acttttgctt ggttcttgtt 60 agtggtacgt gtttctattc aggaaaatca tcgtaactct aattttgaag taataagtat 120 ctttatgaga atggcgtatc gtgatcgtga acgtagtgat agaagagggg gaggaggtgg 180 aagtggcagt aattatagac aacaaaatgg ctattaatta caaaataatt cattttgaat 240 gaatgtttta tgtaatatag taaagaaatg taaacaaact aaagaatgtt ctatagaaca 300 ggttttttta tgtaacgtgc ttctcgcaat ggttaaacac aaagtagagt aaaatgcacg 360 tgatattatg accggttaaa gtaatatgat taaatgtatt agcaagctag gttacgaaaa 420 tctaaggaca ttcataatca ttcttaaaaa aaaaaaaaaa tgaagaaaaa agataaaaag 480 tgtatatgca aatgtaatgt taggtagaat gttactaatg atttttgaca tgtgatactt 540 gcaaatacga ctatatattt catgtacaac ttaaattaat tatttaaaag tcttaatata 600 tttggtacat taaacaaaag taaaacgaaa tgtttataaa atcgaataca aaatattgac 660 aaataatatt ctttaaaata tgggactttt tagaaaatag tnaaaa 706 // ID FN619881; SV 1; linear; mRNA; EST; INV; 672 BP. XX AC FN619881; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-672 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 7f34e7b6f04744baafd3a9e663fde4c8. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..672 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 672 BP; 267 A; 85 C; 113 G; 207 T; 0 other; gcatatatat acatattatg cagaagtgtc tcagtcgttt tattcagaat ttattattta 60 atacattcat aacataaaaa agatagaaaa taatattaca tttgttatta gatcttgcaa 120 ttgtcattat taaataaatg aatcatttga tgaagtctta attccagtac cataatatac 180 ataacctata taaagtttgc tgcttgcaag ttgatttgat catactgtga tacattcctt 240 tctattacaa aaacatgagt gcttataacg taataacatc agattttgta aatgtgtcca 300 ttactaactc tggtcacaac tcatattgca tagaacggcg atttcaaaaa ggaataacaa 360 ttgatgaatt taagggaaaa ttggaacttc ttactggtgg taatccaaca acaatgaaaa 420 ttgaagtata tgacaaaaat gaaaaattaa tttgcgtatt agacaatggt caacgtttat 480 taggatcata tcctattgac gatggaatgc gaatacatat actgtaaaag cattcttgga 540 gaaaaataaa ctaggaaaat ataatgagga agaaatgaaa agaagagcag aagaaaagaa 600 gcaagaagaa gaagcagaaa tagcagctgc acagttatgt aaagttggtg atcgttgtga 660 agtttctgta cc 672 // ID FN619882; SV 1; linear; mRNA; EST; INV; 704 BP. XX AC FN619882; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-704 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 059765748bb308fd24355ef6a5a4ef78. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..704 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 704 BP; 232 A; 121 C; 143 G; 208 T; 0 other; cactaatagt gtacatatag atgtgtatca agcatagcaa taacgttccg ctttttcacc 60 gttacgtggt gatagtgttt cagtacccac aagaaactaa ctaaaaggat taatttgtga 120 acttttgtga ttatgtcttc cgaattagac aactttctta ttaaattcct tttaacctac 180 gaaatacatg tataaagatt tacagatttt attcaacgaa tcgaagcatt agctgaaaga 240 gtatatattt acatatcgat gtacttacta gatcgcaacg ctttgtgatt agtatatcag 300 ttacaggtgg gattattgca ttctgtaatt aataaaatgt cgttagcaag cttattacct 360 acaccatctc aggtcgtgtg ggatagagaa gatgaagcaa gggaacagaa attacgacag 420 agacctgtat ctgctcttgt caaagctgtt gttactgctc caccatatgg ccaacgtaaa 480 ggatttgtgc caagaaaccc agaagatttt ggagatggtg gagcattccc agagattcat 540 gtagcccaat atccacttgg gatgggaata aagggaaaag aaaccaccag taatgcttta 600 gcagtacagc tcgatgctca aggaaaagtg aaatatgatg ttgttgctag acaaggtcac 660 tctaaggata agatcgtgta cagcaaactg agtgacttat tact 704 // ID FN619883; SV 1; linear; mRNA; EST; INV; 667 BP. XX AC FN619883; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G23 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-667 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0fc3bb61c669451ed6edf1cc858974d6. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..667 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G23" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 667 BP; 237 A; 102 C; 137 G; 191 T; 0 other; gcagcgatag ttgacccaga cagtcttctg gatttcaaag ctcttgcaga aggtttagag 60 aaagcactgc cagcatatgc tggaccaatc ttcttgcgaa ttgtgaaaga attggaaata 120 acagggacat ttaagttgaa gaagatggac cttcaaaagg aaggtttcga tccgagcaaa 180 attcaagaca aaatgtattt cttatccggt aacaaagaat acgtcgaaat cacaccagaa 240 ctttatcaag aaattatttc cggatcgaga aagttctagt tgtaacatta acgcttggcg 300 agcgacactc aattgtcgaa tatacttgta aaacgagtac atatataact tttttttaca 360 acatgaatat gactttctgc acgcgccttg tagatacaat gcataggata ttatgtaata 420 acatcaggca tgttaaacca tagaattata ggcggatgtt ttttgaactt atgaaaagaa 480 ggggagagag ttatttttac gagggctcaa aaataggaaa aagagataag cgaaaaataa 540 attacatttt tgtcatcaac aacgggagac gaatatggtg acatgttcct gtttatttta 600 taaatactgt gtatattttt aatcaaacga aacggtccgt agtatgagac aaattggcat 660 tgtaggt 667 // ID FN619884; SV 1; linear; mRNA; EST; INV; 699 BP. XX AC FN619884; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051G24 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-699 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 52578338f6c8ce23e5a89627e55588a9. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..699 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051G24" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 699 BP; 260 A; 103 C; 161 G; 175 T; 0 other; gatacgtagc gctctgtgtt tgtttgcttc ttacggaacg aagctctcga aatatgactc 60 aagaaggcca attgcctccc ccacctgtta tgtgggctca aaggagagaa atattatttg 120 ttactatctg tctagaagat tgcaaagatc ctgcaattaa catcgaacca caaatgatat 180 attttaaagg agtaggtgga actgaacaga aaatgcatga agtaacgatt aacctatatg 240 gagaagttat tccaaacaga actattcaaa acctaagagg aaggactata gagttagttc 300 ttgttaaaaa agaagaaggt ccatattggc caagattaac aaaagaaaaa acgaaagccc 360 attggttaaa aagcgatttt aataaatgga aggatgaaga tgatagcgac gatgaaggtg 420 gaatggaagg aagtggcaac gatttggaag aaatgatgcg acagatgggt ggtctaggag 480 gatcgggaga tagtaaacca aacttcgatg atttagatgc tttaggagat gaaggtgatg 540 gaatggatag cgatgatgac gatctacctg atctgctgga atgaagacat tatgacgcta 600 ctttaaaatt atctacatta aaaaagtaaa aaaattcaaa aacaaatggc gacctaaaat 660 agtgcaccac caggcatatt ttagtaggcc atgctattt 699 // ID FN619885; SV 1; linear; mRNA; EST; INV; 683 BP. XX AC FN619885; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-683 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 549df6e739d3c74c78ff79f918300c49. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..683 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 683 BP; 281 A; 97 C; 125 G; 180 T; 0 other; gctagagtaa atcaaaactt tggtggtttc tcgacgctga aacttgaaag aatcagttga 60 gaaatatatc catggatgga aaacgatcta taaagatgga tccactatca cctggtcctt 120 gtttagacat tagtgacgat gaactagtga ctatatcagt tagagattta aacaggcagc 180 tgaaattgcg tggattatca agagaagaaa tagttcgtat gaaacaacgt agacgcacat 240 tgaaaaacag aggatatgca gccagttgtc gtatcaaacg aattgaacaa aaagatgagt 300 tagaatcaga aaagacacag gaatatcgtg atatggaagc tatgcaggag gacaataatc 360 atatgagaga agaaattgaa tcttggcatt ctaaatatca agcactaaaa aaatttgcaa 420 tagaaaaaaa aattcacatc ccacctgaac tagaaactat ataaaatatt ttaaacatac 480 tagagtcttc tattaaacat aaaagcaata acattactgg gtattgaacg attttatacc 540 aaaattacac aatgcaaaaa actgaattac tagcacttct aagtatgtat taagaattaa 600 gaggaatgtg agcttcatat taatcagaag caatgattgc aatgatactt gggtaaaaca 660 tgatatacta ctatgaataa ttg 683 // ID FN619886; SV 1; linear; mRNA; EST; INV; 662 BP. XX AC FN619886; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H02 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-662 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 4e8f91a2b280c9667b6b2277f04e7201. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..662 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H02" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 662 BP; 194 A; 109 C; 164 G; 195 T; 0 other; ttttagcagt attaggctta gcagctaaag gatacagaat catatcagct gaatcacccg 60 tttattggaa cgttaaggaa tggtgcgatg gattcagaaa acttttagac tacatggagt 120 tagataaggt gcatcttttt ggcacctcat taggtggatt tctggcgcaa aagttcaccg 180 aggcaaatgc tcactgtcct agggtagtgt ccttgattct gtgcaattcg ttcacagata 240 cgtcggtatt taattacaac gattccgcgg gggttttctg gattttaccg tcgttggtat 300 tgaaaaagat gctaatggga aacctcgtga cagacaaggt cgacggagaa atcgtagaag 360 cgattgattt tatggtggaa agactggaaa gtttaacgca accggaattg gcttctcgat 420 tgacgatgaa ttgcgtgaat tgttacgtac agccacaaaa gatatgtcat ttgcctatca 480 cgattataga cgtttttgat gagtatgcgt tatcgaattc ggtgagagag gaagtataca 540 aatgttatcc gaatgcaaag ttggcacatt tgaaaagcgg cggaaatttt ccatatttaa 600 gtcgagcggc tgaagtaaac ttacatttac agattcattt gaggcagttc gacggtactg 660 aa 662 // ID FN619887; SV 1; linear; mRNA; EST; INV; 667 BP. XX AC FN619887; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H03 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-667 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b5c7b721130811bfbc93187840a545a6. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..667 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H03" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 667 BP; 221 A; 96 C; 123 G; 227 T; 0 other; ttgcaatgtt gtgttatgta cctattgttt ggtggttgaa acttatgttt gaaataattc 60 cgtataaaat accttgcaag tctaaaaatc ttaacagtgc ttaatacatc agtttgtttg 120 tttgcttcgt tactcttaat aattattaat aaaatataag aatatttttg gttaagagta 180 gtatcgaaga attaaattcc gtatatgtta acagaataac attacagatg ccatacattt 240 tagtacgtgg taatcttgca tcgtatggtc atagataccc atggagagtt ttagtatcag 300 gtcttaaagc atcagatata gaacaactga gtcgttttgc ttctggggga tattgtgatg 360 attcgacaat agtgtatttg caacatcctt gtgtaatatt aactgcttta gaagttcttg 420 gttacaaagt cgtagcatct tcaagtacaa gtgttaaaca agattataac gagtacatgt 480 ggactatgag aaaagatttt tctgaacctg aacctgaacc tgttattaag acaggtaaat 540 tatcgcgacg ctaattaagc gtacctatca caaacatgga gcacagtcat aactgtaacg 600 atcataatac atgctgcaat ataagtaata atatcggagt tagccagtct cttatggaaa 660 tggaatt 667 // ID FN619888; SV 1; linear; mRNA; EST; INV; 707 BP. XX AC FN619888; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H04 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-707 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; d1d2d77811cb24bdf781f800faa3210d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..707 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H04" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 707 BP; 251 A; 96 C; 123 G; 236 T; 1 other; cgtccatata gagaaatgag ttattccttt catatcacaa tctattttaa ttaagaatgt 60 ggttttcctc gttcagtgtt tattattgac atgtgatgaa ttttatgatt aattttttta 120 taatattttg cataaaattt gaaatataaa taaaataaat aaaatgccga aagtacacac 180 gcaaaaaaca actcgtattc ataaagaagt cgcaaaacag ggaattttat ttaatacgaa 240 tattggacaa catattctaa aaaatccgct tgttattcaa agtatggttg aaaaagcggc 300 agttagacca acagatgttg tattagaaat aggtccaggt actggtaata tgacagtaaa 360 attactggac aaagctaaaa aagtaatagc atgtgaattg gatgctcgaa tggtagctga 420 gttgcagaaa cgtgtgcaag gtacaattta tcagtcaaaa ttacaaatta tgataggcga 480 tgtcttgaaa acagacttac cattttttga tttatgtgtt gccaatatac catatcagat 540 atcatcacct ttagtattca aattactttt acataggcca ctgttcagat gtgctgtact 600 tatgtttcga agagagtttg ctgaacgttt agtagctaaa ccaggagata aattatattg 660 tcgtttaagt ataaatactc agtttgtagc acgagtgnac ttgcttt 707 // ID FN619889; SV 1; linear; mRNA; EST; INV; 680 BP. XX AC FN619889; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H05 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-680 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; a3d8744c88c7b0ebca7a532f365511e4. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..680 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H05" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 680 BP; 217 A; 108 C; 121 G; 233 T; 1 other; attcctgttt tgattaatgg tattatctaa tcatgtgatg cacacttcta aatacctata 60 tataaatata taaaaactaa tgagtttcat ttaagaagta ttatttttta caaataaatt 120 ataaaaaatt tgaaatattt tctacaagtt cttttatgtt tattcaattt aaggatttgt 180 acaggaattg tgattttctt cattttacag tcattttata ttaatataca ggtttttata 240 cgatggttgc ccacactgtg cttcttgact ttacagtacc atcaaatgca atagtagatg 300 tggagaaacg atccaatcta aaaatagcaa ttgctaatgt ccttcaagaa cactttgcta 360 gtctgaagcc attaactgaa tcaagcattg atggaagctt tttagtttta tatacaggtc 420 ccaaaggtag tctaattaca gttcgaagat atactgaagg cttaatcact gttaacattg 480 aatattataa gagagatgaa gaagaagcac ttttggattt tgagttggcc agagaattgg 540 agacggcgtt gcaggcggca gtagcctcta cgcgttcgca ctcgcttaca ccgattaaac 600 gcggcggacc ttttgagaga tactttccta ccgccgatga cagactactt gaatatgaca 660 ttgacnagct ggtttttgag 680 // ID FN619890; SV 1; linear; mRNA; EST; INV; 629 BP. XX AC FN619890; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H06 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-629 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 29643bf5883ac707f59654c5f8aaed36. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..629 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H06" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 629 BP; 230 A; 99 C; 116 G; 183 T; 1 other; acgttgctta ttaatttttg aaacacaaca caaaaatgtc gtacaaagga ccacaaaaag 60 ttcaaaaggt gatggtacag cccatcaacc ttatatttcg gtacctgcaa aatcgttcgc 120 gcgttcaagt atggttattc gaaaacatca acctacgaat cgaaggtcac atcgttggat 180 tcgacgaata tatgaattta gtactggacg atgctgagga atataatctg aagacgaagg 240 ccagaaaacc acttggacga attatgttga aaggcgacaa tataacatta atacaaaata 300 cgaatccagg ggcaaattaa attgttcaac tataacctta agttatgacc caggacaaat 360 aaggcttatt accttatgac ttgtgcaatt acgcacactg gaagtgactt aaaagtgttt 420 aataaagatg gagttaacat tttccaagtt gtacaagttg tgatgcatct ctcggtatag 480 ttaagaagaa caatgattaa ctgatagtta caagttgtaa ctgaaaatac tatcaggtta 540 aagtgacagt attgttttct attgggaaac gtatttgtta ttgtaatctt gncatagaat 600 aatttttcaa atatctgaat gaaaaacta 629 // ID FN619891; SV 1; linear; mRNA; EST; INV; 622 BP. XX AC FN619891; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H07 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-622 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 3bebb90524a3b31f2bab0b3493cc03f5. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..622 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H07" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 622 BP; 238 A; 84 C; 109 G; 191 T; 0 other; ggacaatcgg gctatgcagg agatctgaca tatcgacagt ctttggttga gaggttgaat 60 attataaaac caagctttat gcaaataaag caatttctga cctcacgtcc attaaaactt 120 tcacctggaa ttaaaacact gataacagct ctacaaattc gtgaaaagca agtatttttg 180 atatccggtg gatttcgttc tttaattgca ccaattgcta catgtctgaa tattccatta 240 gaaaatgttt ttgcaaacag acttaaattc tattatacag gagaatatgc aggatttgat 300 gaagatcaac ctactgcaat aaatggtggg aaagcaaaaa taatagaaca actcaaaaat 360 gaaaaaggat ttacaactgt tgttcatatt ggtgatggag cgacagattt agaaactatt 420 tcagtagtaa acctatttat aggatatgga gggaatgtga tcagagagag cataaaacag 480 caatcattat ggtatattac tgattttaat gaacttgtca atattttgta aacgtaaaca 540 atatgatatt tcttataaat attgcgatat ttgaacaatt ttaacaataa ttaataagta 600 aaggatcaaa gtttaaaaaa ag 622 // ID FN619892; SV 1; linear; mRNA; EST; INV; 690 BP. XX AC FN619892; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H08 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-690 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; c5d42766229f0d9872ca207bb4d48d3b. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..690 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H08" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 690 BP; 248 A; 99 C; 122 G; 220 T; 1 other; tttctcgata tagaattgtt tgtaacaatt tgatcggata tgtaaaaaat tattaaaccg 60 aggtttcaat ttgatggaaa aatatcatgc acaatgtctg cgtgcagtaa aaagtcaatt 120 accacgaaga gaatcggaaa gaaggagtca tcctttggca cgtcattgtg atatattaac 180 agctattgaa acaagaatat ctatgttatc catgactttt ataaaatatg tggatcttaa 240 tttgtgttgt ttcattcctg ggaaggtaat cgatgagatt ttttgagttc ttcgtttaat 300 tcgggattct aaaactccac ccagggctca tgaaatactt caagagttaa gagatattag 360 tagcatggct atggaacatt tcgatgaaaa aattttacca gatctaaaac acagtatttg 420 tacttcagtt gttagtaatg taggttctta tgaattgcca agtggaagtc taatgatttc 480 tcatcatact acaagttcaa acagtgtaac actgccacac aattttaatt cagaaaaatt 540 gaatcagact ttcaagaaaa tttatagtcg cactaaaaag aataaattat ctgttctgtc 600 tataaaaggt caaattggta aaatgaaact tcgaatgaaa agacaaggct ttncaatgag 660 attacaaagc ttaaatttac agagcaggca 690 // ID FN619893; SV 1; linear; mRNA; EST; INV; 649 BP. XX AC FN619893; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H09 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-649 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 44b9f939b69de75c3b2aa3eb39ec5e32. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..649 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H09" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 649 BP; 273 A; 91 C; 96 G; 188 T; 1 other; aggttatgtt ctggatcggt cagtacctca gcttgataca tacaatatta atattttaat 60 tttgcttctg tttataaaaa tatgtaaaaa agaattacaa tggctgttag ccgattatgt 120 gcagcatttc gacaaataac aacaaaggga aaaagtaata ttatcgtgag atattactct 180 tcccatgtgt taaaaggatg caataatacc ttgcattttt ctaattttta taaaaatgca 240 ctaactcctt caaacgtgca tattagattt ataccaaaaa cacttgatat agattttaga 300 aatccagaca gtactactaa aaaaagtata tatgaaatac cattatcaag atatatacca 360 tcaatggaag aacctttaat aaaacagcct attaagcagg atttaccatt aacaaataca 420 tcttttgaac tgcctacaac agaaaatatt ttagaaaaat tagctgtacg tttaatggta 480 attagaagaa aaaagatgaa gaaacacaaa agaaggaaac tacgtaaaag aatgaagttt 540 gtgtgggcga agataagagc aaatcgtaat ataacaaagg aaaaagtatt tcaagcagaa 600 ttaattgcaa aaattaaaga agcccacatg ntcaatccta aaaatatgt 649 // ID FN619894; SV 1; linear; mRNA; EST; INV; 735 BP. XX AC FN619894; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H10 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-735 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 7936cda3452a7a5c9ace28f7550e3415. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..735 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H10" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 735 BP; 185 A; 160 C; 208 G; 181 T; 1 other; ctcgatcgtc ggtcgtcgaa agctgattcg tttcgcggta tggcgcacga caacaccgtg 60 gtacgcgtga agcaaggcca gttacgtggc gttatcgagg aaaaccatta tggtgaccgg 120 tatctctcgt ttcgcggtat cccatacgcg aagccgccgg tcgggccgct tcgatttaag 180 gatccagaac ccgtagagcc atggacaggt gtaagggacg ctttagagtt cggcaactct 240 tgctgtcaga aggacatgtt gtcgcacgat ttagtgggaa acgacgattg cctctacttg 300 aacgtgtaca aaccaataaa accgacatcg acgaaaatgt cggtgatggt atggatccac 360 ggtggtgcgt tcatgatggg gtcaggcaac gacgagtatt atgggcccgt gtatttcatg 420 cggaaggatg ttattctggt cacgattaat tacagactcg gtgtcttggg ttttctgaat 480 ttggaacacg aagtcgcgcc gggtaatcaa ggtttaaagg accaagtgat ggccttgaaa 540 tgggtacagg aaaatatcag caactttggt ggagatccca acaacgtcac tatatttgga 600 gaaagcgctg gtgcatcgtc ggtccattac ctaactttat ctccattggc tcagggattg 660 ttccacaagc gattatacag agcggagtgg cgcttaatcc ttgggccnat atagcgagaa 720 ccgagcagta cggat 735 // ID FN619895; SV 1; linear; mRNA; EST; INV; 665 BP. XX AC FN619895; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H11 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-665 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 9f30eeb66b329dca233df25673472420. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..665 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H11" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 665 BP; 222 A; 105 C; 129 G; 209 T; 0 other; actctgttac gtttccacgg tgctaaaaca acaatattta gcgaaatatc gtattgtttt 60 ttatcctata ttgcgttgta aaatacattg cgtcaatcct tctgtaatta tggcttcaaa 120 gtgttttaat ggatgtaatg atcactacaa tggcgtaact attgactcaa atgacgaatt 180 ttgtacatct gaagtgttcg ctcgtcgtct tacagcatct ttacaaaaat ggaaagaaaa 240 taaaaaacgc acaatatggt tccgtgtaca tttaccacaa tcagaatgga tactgttact 300 tgtgaaagaa ggattcaaat ttcaccatgc aaaacaagaa tatgttatgc tttatcgatg 360 gttagttaca gatgaggaat gcaatgttcc acactatgca cacaccaatt taggagtcgg 420 aggatttgtc tataatgagg aaactaaaga aattttagtt atcaaagaga aatatgcaaa 480 tggtccacct atttggaaat taccaggtgg ttatgtggaa ccaggagaag atttagaaga 540 agctgtaaaa agagaagttt tagaagagac tggtgttcaa actaccttta ggtgtataat 600 aggtttcaga cacgttcatg gttacgcccc ttggttgtct gacatataca tgattgccta 660 tttat 665 // ID FN619896; SV 1; linear; mRNA; EST; INV; 684 BP. XX AC FN619896; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H12 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-684 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e465c2be81360db2f9f0bbc73e75c601. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H12" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 684 BP; 233 A; 98 C; 125 G; 228 T; 0 other; gcataagttg tgtctgttta tgtatctatg cgtatgtcat agtgataagt aaatctaaat 60 ttccacgctt ttaaagaaat tctattaatt ataagtcttc gaaagagtac tgcaacaaat 120 acataaatat gtttggattt aacatgtttc cggagattgt cagacctttc aataaccact 180 atagatgttt ttctgtgtca atgttaccgg gtacatatcg acgagatgta gaacgtggag 240 ggaaaataat catgccaccc tcagctttag agcatctcac aagattaaat attagatttc 300 ctatgttatt cagattaagt aacgaaaaaa ccaatagaat tactcattgt ggagttttag 360 agtttgttgc ggacgaagga agagtttatt taccttgttg gatgatgtac aatcttttgt 420 tacaagaagg agaattaata aatgttgaaa gtgtaaattt acctgttgca acattttcac 480 gttttcaacc acaatccgaa gattttttag acatcacaaa tcctaaagct gttttagaaa 540 atggattaag aaattttgct tgtcttacaa caggtgacat tattgctata aaatacaatc 600 aaagagtata tgaaatgtgt gttttggaaa caagaccagg tccagcagtc actatcatag 660 aatgtgatat gaacgttgaa tttg 684 // ID FN619897; SV 1; linear; mRNA; EST; INV; 666 BP. XX AC FN619897; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H13 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-666 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0ce283c155a545cb03a7bf03c2fd8e9f. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..666 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H13" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 666 BP; 249 A; 106 C; 118 G; 193 T; 0 other; caatgaagcg tcagcacgta aaatggttga ttttctttca agcgttatac cttgtcgcta 60 tgaccattct aaaaaactga tatcacatga tattcacagc aatatctaca attataaaat 120 tacatacagt gtggaaatag ttccaatatc aagagatagc atagtatgtc ttccaagaaa 180 actaagtcat caacttggag ggataaattc aatttgttta gtatataaaa ttacaaattt 240 tgtacattta atagatgtat caactggcca aattgcagaa ttaagcgcta caccttactg 300 gagacatact ttcaacagca tttgtgaccc aaagcaatta atagaataca cagtaatgga 360 tattgagcaa ataaaattca aggatagaaa attttttcct ggacagggaa ctatttcaaa 420 taagcatgtt atagcagatg tatgggtagt tagaagttgc gatttgggac tgactgagaa 480 cttaattcat acatgcactc accttggcca tatattaaaa cctggtgata ctgtcctagg 540 gtatgctctt aatgagagta acattaacga tgagaatttc gaaatgttga ataaagacgt 600 agtaccagac gtaattttaa ccaaaaaaaa ttatacacaa gatagagcag cacgtcgcag 660 aatgag 666 // ID FN619898; SV 1; linear; mRNA; EST; INV; 662 BP. XX AC FN619898; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H14 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-662 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 9658566b2a8d24ad8c5e3e24cfa61633. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..662 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H14" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 662 BP; 253 A; 69 C; 94 G; 246 T; 0 other; acttataaca cgcgaatctg caagagttgt atttcaagct ctacatgttg aacgttgggc 60 atttgatgta gaaatgttat atattgcaag aattctaaac attcctatta ttgagattcc 120 tgtgaattgg acagaaatta aaggatccaa aatagtccct ttctggagtt ggttacaaat 180 gggtaaagat ttatttttca tttggtataa gtatagaatt ggggcatgga aaataaaaag 240 atctaaacaa acgtaatata aatgtgcaca gaactcatat tatttataaa taaacctttt 300 attcgatatt tccactatag ataacattag atatcatgtt taacataatt agataatata 360 atttacatag tgatcatgaa atatttttcc tttgtaagga aaatttataa atgtgaaaaa 420 ccataacaag ctttatttaa taagcttata taatctttat tttaatgaat aattatataa 480 aaaagtatga tagattatca taaatgtttt taaagagaaa gagagctgtt tggtgtttta 540 gattaactta atcaaattga gcaagatata ttacttcgtt tatatattta ttcttttatc 600 tattatatgc attgtaatat gtaatgatca ttgttaataa ataacacttt ataaagaagc 660 at 662 // ID FN619899; SV 1; linear; mRNA; EST; INV; 659 BP. XX AC FN619899; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H17 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-659 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 1435e49a213b6b1158e7a5a5e9e73f0d. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..659 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H17" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 659 BP; 210 A; 121 C; 129 G; 198 T; 1 other; tctgaacgtg atgtcggcag taatcattgt tcgtgattag ttatattact gtgttagcct 60 gtagtggcat ggttttcgag tatttacgcg atacgtgtat catgtgcgcg tattgatgtt 120 gtatttggaa tgcgcggtac attgagacaa atcctgtata tcacatttat tccattcgtc 180 aattgatcaa ttgaagaaca cactagcgaa agcttaaact tgcttataac agactacgaa 240 attacatttg tatacacaca tatatataca tatacaggta tatatgtata tacatgtacg 300 tggatataca catacatggt gtcgtgtttc atcgtttcac gttataccga gtactggata 360 taaacatttt tcaatggaat gataggcgtg tcaccatacc agacaaatgt gtgctagcac 420 taatagcata tcatcaacgc actcgttgcc gttaaacaat taaagaagag gcagacttca 480 aaacatactt acaggcctag gtgtggtttt aataacttct aatataaaat ggcagaagag 540 tgcaacgatg ttaagtctgc tggtccatct tcaccaagaa gtccactgaa taatagtatg 600 ggaggaaaac caagcagtcc tcattctcct agacnaccaa ggcgtatgaa gctaacaca 659 // ID FN619900; SV 1; linear; mRNA; EST; INV; 637 BP. XX AC FN619900; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H18 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-637 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; fbd8cafe14d504b7a9dca60ad0fc61e0. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..637 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H18" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 637 BP; 238 A; 73 C; 149 G; 176 T; 1 other; ttacgtgttt ttagtattgc gtaaaggact attttattac aaagatatta tttaaagata 60 tagaaaatgg ctgtagacat atctccgaac aaggatggag gtgtacagaa agaaataatt 120 aaggaaggta taggagatga aactccatca cctggaagca acgtaatagt acattatact 180 ggtaccttaa tggatggaac aaagtttgat tcaagcaagg atcgtaatga accatttcaa 240 tttgaactta agaaaggaag tgtaattaaa gcatgggata taggggtagc tactatgaaa 300 aagggcgaag ttgcactatt gacctgtgct cctgaatatg catatgggaa aaatggcagt 360 ccacctaaaa ttccttcaaa tgcgacactt aaatttgaaa ttgaaatgat tgattggaaa 420 ggagaagatt taagtcctga aaaaaatggt agtattgaaa ggtatcagat tgtacaagga 480 aaagattata ttactccaca agaaggagct ttagtaaatg tacatttaac tggaatgtat 540 aatgggaaag tatttgaaga tagagatgta caattctccc tcggagaagg agaagattgt 600 ggtgttatag agggtgtaga anaggctttg gagagct 637 // ID FN619901; SV 1; linear; mRNA; EST; INV; 650 BP. XX AC FN619901; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H19 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-650 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 63eaa75987cdb39f3bd1e0b78e933b66. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..650 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H19" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 650 BP; 164 A; 136 C; 148 G; 201 T; 1 other; gcgcgaacgt tttcatccgc actacagagg gtaacacata agattctgcc tgctttaacg 60 gcagtctttg ctcgctgttt gacaagaaag aagactagtg ttctagccgt tgcaagctta 120 atcgctatgg ttaatcaggt tgcctccttg tcctatatag tggagaacaa tgcaggcaag 180 gaaagcgctg cagatttcga aaaagccatc aaactgtgtg ggtacggaaa attccactat 240 ggcttcgtat tactctgtgg agcaatgttc ctctgcgttg gttgtcagaa cggaataaac 300 gcgtacattt taccatccgc agagtgtgat ctcgatttga gctctgaaca aaaaggtctt 360 ctgaacgtcg ccttcctctt aggcggagtt gtaagctccc ttttctgggg agtatttgca 420 gatgcctacg gaaggagata tattctcctc cttactttat taagtgacag tattttgtca 480 attggaggaa gtttctctca aagctttaaa gtgctgttaa ttttcagagc acttagtgga 540 ttcttcatgg gtgctcctgg ttctctagtt tatacgtatc ttggtgaatt ccacgcagcc 600 aaacatagag taaaagctat ctgttacgta ngattcttct ggaccctatc 650 // ID FN619902; SV 1; linear; mRNA; EST; INV; 667 BP. XX AC FN619902; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H20 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-667 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; f44f39e99ab104131b1fa69d903a6920. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..667 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H20" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 667 BP; 233 A; 104 C; 131 G; 199 T; 0 other; atcgaactgt gacttaccac actggttgcc atttcggttc tcctggaatt agggtcggca 60 aaaaagcata caatggcaaa taaggtattg ctatcgtgtt tgcgacaaaa tactccaaag 120 tatgcaaatt tcattcgtgg attatcagga gtatctagag ttcctgacaa aattggtaat 180 agggatatag taggtcatgg ttggaatgga gaaacaactt acttagatcg atgcgatttt 240 cctttgcctg caattcgttt caaaccaaat actccagaca taatggcatt gcgagaaaaa 300 gaaaaaggtg attggaagaa attatcaata gaagaaaaaa aggcattata tcgagccagt 360 ttccgtcaaa catttagtga atttcaagcc tcaacgggag aatggaaagg acatattgga 420 attgcactca ttggtgtggc ttttagtctt tgggtagtaa tgtttctcaa aacttttgca 480 tacccgccat taccagaatc atttaatttg gaaaacagac ttgcacaact ggaacgtatg 540 aaaatacttg aagtcaatcc tattagtgga gttagcgcaa aaaaataaat ttaatctgct 600 tatgtttaaa acatagtaaa aaatatagtg tattatacac taataattgt taatataaat 660 gttctgt 667 // ID FN619903; SV 1; linear; mRNA; EST; INV; 690 BP. XX AC FN619903; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H21 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-690 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; e1185e8c9c36b0d1f3ef6e114e31a027. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..690 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H21" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 690 BP; 237 A; 111 C; 127 G; 215 T; 0 other; tggcgtcggc ggatgttgaa cgtgctaaga tggaatctac cctacgtaat ttaaaattgt 60 ctggtcatgt tggattcgac agtcttccag atcaattggt aaataaatcc gttcaaaatg 120 gatttgtatt caatattctt tgtatcggtg aaactggact tggaaagtcg actcttatgg 180 attctctatt taatacaagt tttgagtcta tgccaagccc acataatctc cctgctgtaa 240 aacttaaagc acatacctat gaattgcaag aaagtaacgt taggttaaag ctcactattg 300 tcgatacagt tggttacgga gatcaaatta ataaggagga cagcttcaaa gctgttgttg 360 attacataga tgcccaattt gaagcatatt tacaagagga attgaagata aaacgatctt 420 tgccaactta tcatgacagt cgtattcatg tttgcctata ttttatttgt ccaacaggac 480 acggattaaa gtcaattgat ctagtctgta tgaaaaagct ggatacgaaa gtcaatatta 540 ttccaataat tgccaaagct gatacaatat ctaaaacgga attacagaag tttaagacta 600 aaattatatc tgaactacaa aataatggag ttcatattta tcaatttcct acagacgatg 660 aaaatgttgc agaagtaaat actagtatga 690 // ID FN619904; SV 1; linear; mRNA; EST; INV; 709 BP. XX AC FN619904; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H22 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-709 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; b88625222c82dff755f38579d55d6fa0. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..709 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H22" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 709 BP; 205 A; 133 C; 160 G; 211 T; 0 other; cgagatttcc taggtacgag caactacgtc caagcaagcg aaagcagaga aggtgcgtaa 60 taaataaacg agtgatcgag ttagcatcat tccatcgaag atgacgagac tatcgctctt 120 tctactcctt cccgctcttg tgactctgac atagaactta gaggatgcgc ctaagctaaa 180 gattcctttg agtgcgatta agagatatag tgttcgccga tggaagacag tacgaagcct 240 atgaaggaat tccttatacc ttgccgccaa tcggtaaatt tcgattcaag tctttccaaa 300 ggctaccatc atgaatgggc gagcttccgg cgacaaaatt tggattccct tgtatgcaat 360 attcttaatt gcctcaagat ccgaatgata gagccgagag tgccgaaaat tgtcttattt 420 gaatatttat gcgctggtag atagaagaac atctgaatcg ttatcagata ctttctggat 480 ccatggcggt gcttttcaat taggtaacga atgtttatag tgccaaatac ctcatggatc 540 gtgacgttgt atttgttaca ttcaattatc gtttagaaat gttaggtagt tgtatatttt 600 taacagcgac agtagctgta gtttcttaag gtatcctcag cactgaagat ggcataattc 660 ttgggtatat gagactgaag ggatcaagta tggcactacg atgggtttc 709 // ID FN619905; SV 1; linear; mRNA; EST; INV; 679 BP. XX AC FN619905; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H23 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-679 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; c3518eabeb946b34ce2d7275ef14b5a7. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..679 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H23" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 679 BP; 213 A; 134 C; 127 G; 204 T; 1 other; gtgagtgagc gtgcgtgcgt gcgagcaagc gagcgcgcaa ccaaacgata tagttcgaaa 60 tagttgtgca caagctgtgt aaacggtgtt ggcacgcgta gctgatcaaa ccgatcgaga 120 tttgcgtgtg gtttaacttc gccgtactat atcatcgcct tgtgatcaaa cctttctact 180 ttcaacgcac tcaacggtaa caagcatgat ccttcgaatt tcctccgtct tgttcctcgt 240 gcgtacatca ttacacgatc gttcgacgaa gaatacgcag tgtagaaacc agagcaagta 300 gtttacacca gtgagcgcaa tctaaggacg ttcgtgattt attttgtttt tgaattcttt 360 aacgctatac tattactaca tcgttatgcc attagcattt ctaataacga tggttaatct 420 gaaatcaagt atcgtgcttt cctgtaacag cgacaaaatt atattaaaaa agtgaaatat 480 caatatttcg aagaaagaag aactttacta tcgaatacct gtacacgcat tcgtgtacaa 540 tattattgct aacacagcac cgattttctt tatactagtg tacatacata tgtgtaaggg 600 aaacgtatat atatacatga atattttctc tgtaaaatgt aaaaaaatca taaccnacga 660 tgttgaaaac gtatactac 679 // ID FN619906; SV 1; linear; mRNA; EST; INV; 675 BP. XX AC FN619906; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051H24 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-675 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 0546f4afcab0987c54cf001003002d93. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..675 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051H24" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 675 BP; 231 A; 148 C; 131 G; 164 T; 1 other; gcacctacat taacaagagg ctggaggtgg taaacctgga aaatacacac aagctaaggg 60 attcctttct tattacgaaa tctgtaccaa tttacaagct ccaggcagta actggactca 120 aaaatttgac gatattggca aagttccata catgtatcaa ggtacacaat gggttggtta 180 tgaaaacgtg gagagcgtga aatacaagat cgattttatc aaggaatctg gatacgctgg 240 tgccatggtt tgggccatcg acatggacga ctttcaagga ctttgcggtg accgtaaccc 300 tctgatgaat gtcattaatc aaggtttaga aggatatatc gtgccagata aagattttca 360 taccactcct acgcctgact gggcaaagcc accaagtacc atatcgtcag ataatattca 420 aactactcaa agtagccaaa ttactcaatc tgcacgaccg tctacaacta acaaacctac 480 aaaaagacct actcagaagc cagaggtaac ttcttccact acggaaacaa ataaatccga 540 atctacgcaa cagccgatag acaatgacga agtacataaa atcaattgtn gaaacgctaa 600 taaattcgtc ccgtctaaag actgtcactc ttactatgag tgtgttcacg gaataccagt 660 gaaagttcag tgtcc 675 // ID FN619907; SV 1; linear; mRNA; EST; INV; 675 BP. XX AC FN619907; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051I01 XX KW EST; expressed sequence tag. XX OS Bombus terrestris (buff-tailed bumblebee) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Hymenoptera; Apocrita; Aculeata; Apoidea; Apidae; OC Bombus; Bombus. XX RN [1] RP 1-675 RA Beck A.; RT ; RL Submitted (15-DEC-2009) to the INSDC. RL MPI for Molecular Genetics, proScience, Ihnestrasse 73, D-14195 Berlin, RL GERMANY. XX RN [2] RX DOI; 10.1186/1471-2164-11-110. RX PUBMED; 20156341. RA Sadd B.M., Kube M., Klages S., Reinhardt R., Schmid-Hempel P.; RT "Analysis of a normalised expressed sequence tag (EST) library from a key RT pollinator, the bumblebee Bombus terrestris"; RL BMC Genomics 11(1):110-110(2010). XX DR MD5; 4b34dd073ef6b77948b8b3125be6f27c. XX CC Lab-raised worker offspring of field caught spring queens were used. CC Total RNA was extracted from 2 female worker offspring from each of 4 CC colonies. cDNA was synthesized using the SMART approach (Mint-Universal CC cDNA synthesis kit, Evrogen, Russia), subsequently normalized using CC duplex-specific nuclease (Trimmer kit, Evrogen, Russia), and directionally CC cloned into pAL32 plasmid vector (Evrogen, Russia). Plasmids were CC transferred via electroporation into E. coli DH10B (Invitrogen, U.S.A.). CC Plasmids from the normalized libraries were 5' end sequenced using the CC pALforward primer and Big Dye Terminator chemistry (ABI). Collected reads CC from 3730XL capillary sequencers (ABI) were base-called using Phred CC (http://www.phrap.org). Library construction and sequencing were CC performed at the MPI for Molecular Genetics Berlin. XX FH Key Location/Qualifiers FH FT source 1..675 FT /organism="Bombus terrestris" FT /mol_type="mRNA" FT /country="Switzerland" FT /lat_lon="47.46 N 7.57 E" FT /collected_by="Sadd B.M." FT /collection_date="Jun-2007" FT /sex="female" FT /dev_stage="adult" FT /clone_lib="bom001no" FT /clone="bom001noP0051I01" FT /tissue_type="Thorax and abdomen" FT /db_xref="taxon:30195" XX SQ Sequence 675 BP; 235 A; 126 C; 152 G; 162 T; 0 other; atttggtcca cggtggatca tcaactgaaa gaacgatttg ctgatttacg ggacggagca 60 cgaatcgtat cgtcaaaatc attttgcccc ctcaactttc gcataacgga tagaaatctc 120 agtgatattg gtacgataat gcatgtatca gagatgtcac cactaaaggg ttctgtctcg 180 tggactggta aaccggtgtc ttattattta catgtaattg atcgtactaa gttagaacgt 240 tatttccacc gcttaaagaa taacaagcaa ggtggcgatg aaaattctga ttctgtgata 300 aataacactg ccaacaataa caataaggtt acaaatagag gtgaaagagg tgatagagct 360 aaacgggatc tttcaaggca gttagaaagt ccgaatcatt cggagcaaca aggagcaaat 420 agcgattctg atgcagatga aaataacatt aaaaatcgtc ggcaatcaac taagatccgt 480 agaaaaataa gtaggaaatc aagtaatata agacccccgg ctagaggcag acagagggga 540 agaggcgtaa agaaatcaaa gcccaagaaa gcaattaata tttctggttt ggacttactg 600 catagtcaaa cattaccgag tacatctccg caagctttgg gaaagaaacc accgcccgcg 660 ccctggtgtg tggat 675 // ID FN619908; SV 1; linear; mRNA; EST; INV; 701 BP. XX AC FN619908; XX DT 30-JUN-2010 (Rel. 105, Created) DT 30-JUN-2010 (Rel. 105, Last updated, Version 1) XX DE Bombus terrestris EST, 5' end sequence, clone bom001noP0051I02 XX KW EST; expressed sequence tag. XX O