ID JK123687; SV 1; linear; mRNA; EST; MAM; 677 BP. XX AC JK123687; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO11FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-677 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 296330f4b204bd2d3ba9d3678ad85a73. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3116 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000012120 (LOC100405245) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000026980 CC High quality match with Homo sapiens transcript: ENST00000394046 CC (AC069137.1-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000019411 (XM_001103181.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000005939 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000007901 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000023353 (XM_526099.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000014991 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X19WM02F7O9Y, FZOOKC302II8MV, F0X19WM01DPBA0, F1RHTUQ01AOFBW, CC F0X19WM01CB48E CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874255|FR874255.1:32037791-32037643. XX FH Key Location/Qualifiers FH FT source 1..677 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 677 BP; 233 A; 109 C; 169 G; 166 T; 0 other; gggagctgcg tctggagctc ggctgttggc tggagtgggc gcttatctgg tgtggggaag 60 gcggcgggac tcaggcctgc ctgcgaagca ttgtcctaca taatggtaga ggatgaactg 120 gcactgttcg ataaaagcat aaatgaattt tggaataaat tcaaaagtac ggacacctcc 180 tgtcaaatgg cgggactaag agatacctac aaggactcca tcaaagcatt tgcagaaaag 240 ctgtctgtga aattaaagga agaagaacga atggttgaga tgtttctgga atatcaaaat 300 cagatcagga ggcaaaataa gctcattcaa gaaaaaaagg ataacttgtt aaaattgatt 360 gctgaagtaa aaggcaaaaa gcaggaattg gaagtactga ctgcaaatat ccaggatctt 420 aaggaagaat attctaggaa gaaggaaact atttctactg ctaataaagc gaatgcagag 480 aggttgaaaa ggctgcagaa atctgcagac ttgtataaag atcgacttgg actagaaatt 540 cgaaaaattt atggtgagaa attgcagttt atattcacta atattgaccc taagaatcct 600 gagagcccat ttatgttttc cctgcatcta aatgaagcaa gggactatga agtgtcagat 660 agtgcccctc atcttga 677 // ID JK123688; SV 1; linear; mRNA; EST; MAM; 500 BP. XX AC JK123688; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO13FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-500 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 09ba0c0b99311041c5258531495d473e. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000019668 (LOC100407011) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000022481 CC High quality match with Homo sapiens transcript: ENST00000306735 CC (RBFA-001) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000010721 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000018595 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1HESOY01CFLE4, F1BDRJG03HIKM1, F1IUBWX01B5SFU, F0DRJ6O01A8PEU, CC FZ8A1BE01AN517 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874261|FR874261.1:73614527-73615026. XX FH Key Location/Qualifiers FH FT source 1..500 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 500 BP; 124 A; 115 C; 160 G; 101 T; 0 other; aaagataaag ggctcggggg cctggtgtgg caggggcagg cggctgagct gacaacgcag 60 atgaaaaagg gaaggaagag ggccaggccc cgcctggagc aggacagctc cctcaagagt 120 tacctgtcgg gcgaggaggt ggaagatgac ccggacctca ttggtgcccc ggagtacgaa 180 tgctacgccc tggacacgga ggagttggag gcagagaaag gagatggcac aacggaggat 240 ggccacggct gcaatgcaag cagggagtag atggagaggc gtcagctctg cccgtcccac 300 gttcgcaggg aaaagcattg gcacgtgccg ccgcatgtgg ctttgtcgag gcagttgatg 360 gagttaaaac catctgctct tctgttacgt cgacgttttc tagcttttcc gtgtatctaa 420 acacagtttg ctgcagaagt cactgttgtt tttttccata cactgtgtgt aatgtaaaaa 480 ataaatggcc atctcaccac 500 // ID JK123689; SV 1; linear; mRNA; EST; MAM; 740 BP. XX AC JK123689; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO14FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-740 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; bc105d93ff77b367c007f03117484f99. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000054686 (A6MKZ1_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000033663 CC High quality match with Homo sapiens transcript: ENST00000542520 CC (TOP2B-204) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000010316 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000004796 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000027495 (XM_516332.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000016353 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF01EQFB2, F01W12W02FQGFD, F0OTZ2I02G3LV1, F0BOF8H01BSKSS, CC F0X19WM01C1J7U CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874245|FR874245.1:168933212-168933159. XX FH Key Location/Qualifiers FH FT source 1..740 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 740 BP; 261 A; 137 C; 140 G; 163 T; 39 other; aagaaacaga acctgtggtt attccaagag attctttgct taggagagca gccgctgaaa 60 gacctaaata cacatttgat ttctcagaag aagagnnnnn nnnnnnnnnn nnnnnnnnnn 120 nnnnnnnnnn nnnnttagag gaattgaaag ttaaaacatc tcccataaca aatgatgggg 180 aagatgaatt tgttccttca gatgggttag ataaagatga atatacattt tcaccaggca 240 aatcaaaagc cactccagaa aaatctttgc atgacaaaaa aagtcaggat tttggaaatc 300 tcttttcatt tccttcatat tctcagaagt cagaagatga ttcagctaaa tttgacagta 360 atgaagaaga ttctgcttct gttttttcac catcatttgg tctgaaacag atagataaag 420 ttccaagtaa aacagtagct gctaaaaagg gaaaaccgtc ttcagataca gcccctaagc 480 ccaagagagc ccccaaacag aagaaagtag tagaggctgt aaactcggac tcagattcag 540 aatttggcat tccaaagaag actacaacac caaaaggtaa aggccgaggg gcaaagaaaa 600 ggaaagcatc tggctctgaa aatgaaggcg actataaccc tggcaggaaa acatccaaaa 660 cagcaagcaa gaaaccgaag aaaacatcct ttgatcagga ttcagatgtg gacatcttcc 720 cctcagactt cccttctgag 740 // ID JK123690; SV 1; linear; mRNA; EST; MAM; 758 BP. XX AC JK123690; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO15FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-758 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 7d2560de765e169a0e0a1d643c122b11. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000035844 (LOC100413160) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000003204 CC High quality match with Homo sapiens transcript: ENST00000366639 CC (TSNAX-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000022764 (XM_001104491.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000010349 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000002748 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000005577 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000003859 (XM_514271.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000000122 CC (TSNAX_PONAB) CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000014070 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF02F0MES, FZ8FYPF02HW5WE, FZ8FYPF02IFPAP, F1HESOY01BC5F0, CC F0DRJ6O02I9PTP CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:226706584-226706470. XX FH Key Location/Qualifiers FH FT source 1..758 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 758 BP; 247 A; 128 C; 173 G; 210 T; 0 other; cctttaaatc atttcagcag gaacttgatg caaggcatga caaatatgag agacttgtga 60 aacttagtcg ggatataacc gttgaaagta aaaggacaat ttttctcctc cataggatta 120 caagtgctcc tgatatggaa gatatattga ctgaatcaga aattaaattg gatggtgtca 180 gacaaaagat actccaggta gcccaagagc tatcagggga agatatgcat caattccatc 240 gagccattac tacaggacta caggaatatg tggaagctgt ctcttttcaa cacttcatca 300 aaacacgatc attaattagt atggatgaaa ttaataaaca attgatattt acgactgacg 360 acaatgggaa agaaaataaa actccttcct ctgatacaca ggatgagccg tttggtactt 420 ggagactgag agtcacacct gtcgattacc ttctgggagt ggctgactta actggagaat 480 tgatgcggat gtgtattaac agtgtgggga atggggacat tgacaccccc tttgaagtga 540 gccagttttt acgtcaggtt tatgatgggt tttcattcat tggcaacact ggaccttacg 600 aggtctctaa gaagctgtat accttgaaac aaagtttggc caaagtggag aatgcttgtt 660 atgccttgaa agtcagaggg tcagaaattc caaaacatat gttggcagat gtgttttcag 720 ttaaaacaga aatgatagat caagaagagg gtatttct 758 // ID JK123691; SV 1; linear; mRNA; EST; MAM; 727 BP. XX AC JK123691; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO16FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-727 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2c6343fcb76bb21658154cceab072529. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1963 CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000038267 (YP_026110.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H01CLXFH, F1HESOY02FVHNT, F0Q2XTE02G2TMC, F0BOF8H02HSN53, CC F0Z8TLB01D693I CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:109604756-109605385. XX FH Key Location/Qualifiers FH FT source 1..727 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 727 BP; 203 A; 233 C; 97 G; 194 T; 0 other; cttatcacat agtcaaaccc agcccttgac cactaacagg agctctgtca gcccttctaa 60 taacatccgg cttagtcgtg tgattccact tctattctac cactctattg acactaggcc 120 tactaaccat aacactaacc cttcaccaat gatgacgcga tgtcgtacga gaaagcacct 180 atcaaggaca tcacacgaca cccgttcaaa aaaaccttcg ctatggcata gtcctattca 240 ttatctcaga aatcttcttc tttaccgggt tcttctgagc attctaccac tcaagcctag 300 ccccaacccc ctatttagga ggccactgac caccaacagg tattacccca ttagatcccc 360 tagaagtacc ccttctaaac acctccgtat tattagcatc aggaatcaca attacctgag 420 ctcaccacag ccttataaac aacaatcgaa aacaaacaac ccaagcccta ctcattacaa 480 ttctactagg catctacttc accctactac agatctcaga gtactttgaa gcacccttca 540 ccatctccga cggcatctat ggctcaacat tctttatcgc tacgggcttc catggactcc 600 acgtcatcat tggatccact ttcctcctta tctgcctcat tcgccaacta ttattccact 660 tcacaccaaa tcaccacttc ggctttgagg ctgccacttg atattgacac ttcgtagacg 720 ttgtatg 727 // ID JK123692; SV 1; linear; mRNA; EST; MAM; 676 BP. XX AC JK123692; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO17FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-676 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f9f379c844b3649feb00e14d7c3e7a6f. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RTSFR01CTTK9, F0BOF8H01E10LR, F0BWDJC01DG1ZD, F1GITI001DKEUG, CC F0Z8TLB02JDLKH CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874245|FR874245.1:190566802-190566843. XX FH Key Location/Qualifiers FH FT source 1..676 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 676 BP; 181 A; 124 C; 136 G; 235 T; 0 other; ccaaagtgct gggattacag gtgtgagcca cctcgcccgg cctccatcac tgagtttatt 60 tcccacaatg ttatataaat ggaatcaaat agtatgtact ctttttcaaa acctagctct 120 ttttattcac tcagcttact catttaagat ttatttctgt tgtttggtgt gttttctgtt 180 ttactgctga gtagtactct gttgtttgct tgtaccacag ttaatttttc cattcacctg 240 ttgacaaaca tttccaggtt ttggctatca caggttaggt tgctgtgaac atttgtcttt 300 gtgtggcttc ttatgtcgtt ttatgtttgc agtaacccac taaggtaggt ggataaactc 360 atttcttttt gcagacgaag aaatgaatgc tcagtgaggt taagtaactt gcccaaagag 420 gtaggaagtg gcaggggcaa gaattcgaat atagaattct ggaatcttgg gcatcaaagc 480 tcttgtgatg ccagactgca agatgctaca caaataggca ttaacaccaa ctggaatgtt 540 gttgttagaa agcttattat catgtagaat ggtataagat gattcctccc ccataagatt 600 ttaaccccag aagatttttg aatgacactc tatacactta tttttcatgg agaagatgac 660 tatttgactc ttaggc 676 // ID JK123693; SV 1; linear; mRNA; EST; MAM; 542 BP. XX AC JK123693; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO18FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-542 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f4c4f1cc76373f9af2d0ecbc12f0aff0. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1145 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000022649 (B0VX78_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000028499 CC High quality match with Homo sapiens transcript: ENST00000320868 CC (HBA1-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000000859 (HBA_MACMU) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000010223 CC High quality match with Pongo abelii transcript: ENSPPYT00000008138 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB01EFJPF, F0DRJ6O02GRH4D, F0Z8TLB01EFJPF, F0DTF6N01BZQPC, CC F0M2C4T01BK5LM CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874263|FR874263.1:133866-134105. XX FH Key Location/Qualifiers FH FT source 1..542 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 542 BP; 85 A; 202 C; 153 G; 102 T; 0 other; caccatggtg ctgtctcctg ccgacaagag caacgtcaag gccgcctggg gtaaggtcgg 60 cggccacgcc ggcgagtatg gtgcggaggc cctggagagg atgttcctgt ccttccccac 120 caccaagacc tacttccccc acttcgacct gagccacggc tctgcccagg ttaagggcca 180 cggcaagaag gtggccgacg cgctgaccct cgccgtgggg cacgtggacg acatgcccca 240 cgcgctgtcc gcgctgagcg acctgcacgc tcacaagctt cgggtggacc cggtcaactt 300 caagctcctg agccactgcc tgctggtgac tctggccgcc cacctccccg ccgagttcac 360 ccctgcggtg cacgcctccc tggacaagtt cctggcttct gtgagcaccg tgctgacctc 420 caaataccgt taagctggag cctcggtggc catgcttctt gccccttggg cctcccgcca 480 ggccctcctc cccttcctgc acccgtaccc cctggtcttt gaataaagtc tgagtgggcg 540 gc 542 // ID JK123694; SV 1; linear; mRNA; EST; MAM; 150 BP. XX AC JK123694; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO19FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-150 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f682b7b66764e4b47e66e7474b96e5e3. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig385 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000055100 CC High quality match with Homo sapiens transcript: ENST00000545844 CC (MACF1-207) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000013355 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000015201 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000000073 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000050795 (XM_001170848.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000001801 CC (Q5R857_PONAB) CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000011131 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF01DRNJB, F0ZWYBK02H44C1, F1RHTUQ02JP53X, F0X5I7101CIXMN, CC F0X5I7101AFHRU CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:42383986-42384025. XX FH Key Location/Qualifiers FH FT source 1..150 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 150 BP; 39 A; 35 C; 44 G; 32 T; 0 other; tgaagcaaaa aacccacgga tcaaccagct ttctgcccgc tggcagcagg tgtggctctt 60 agcactggag cggcaaagga agctgaacga cgccttacat cggctggagg agttgaagga 120 atttgccaac tttgactttg atgtctggag 150 // ID JK123695; SV 1; linear; mRNA; EST; MAM; 642 BP. XX AC JK123695; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO20FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-642 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 7054a865c206e28315e1388222833ec2. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1180 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000031092 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000023460 CC High quality match with Homo sapiens transcript: ENST00000391977 CC (EEF1A1-202) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000029930 (Q3YAI9_MACMU) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000017692 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000005994 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000009153 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000048233 (EF1A1_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000019682 CC (EF1A1_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX02FWVEF, F1HESOY02F8KHC, F0BWDJC01ETLJR, F0Q2XTE02HN1F2, CC F0X19WM02FHTH1 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:65830702-65830061. XX FH Key Location/Qualifiers FH FT source 1..642 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 642 BP; 164 A; 152 C; 166 G; 160 T; 0 other; cttacattaa gaaaattggc tacaaccccg acacagtagc atttgtgcca atttctggtt 60 ggaatggtga caacatgctg gagccaagtg ctaacatgcc ttggttcaag ggatggaagg 120 tcacccgtaa ggatggcaat gccagtggaa ccacgctgct tgaggctctg gactgcatcc 180 taccaccaac tcgtccaact gacaagccct tgcgcctgcc tctccaggat gtctacaaaa 240 ttggtggtat tggtactgtt cctgttggcc gagtggagac tggtgttctc aaacccggta 300 tggtggtcac ctttgctcca gtcaacgtta caactgaagt aaaatctgtc gaaatgcacc 360 atgaagcttt gagtgaagct cttcctgggg acaatgtggg cttcaatgtc aagaatgtgt 420 ctgtcaagga tgttcgtcgt ggcaacgttg ctggtgacag caaaaacgac ccaccaatgg 480 aagcagctgg cttcactgct caggtgatta tcctgaacca tccaggccaa ataagtgctg 540 gctatgcccc tgtgttggat tgccacacgg ctcacattgc atgcaagttt gctgagctga 600 aggaaaagat tgatcgccgt tctggtaaaa aactggaaga tg 642 // ID JK123696; SV 1; linear; mRNA; EST; MAM; 626 BP. XX AC JK123696; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO21FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-626 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 6b3d30ced6dbb47e41fafa97b9f7d01f. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000019766 (INPP5A) CC High quality match with Homo sapiens transcript: ENST00000536599 CC (INPP5A-204) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000005386 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000002512 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000048486 (XM_001155993.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000003354 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X19WM02F2F0G, F0Z1IVZ02JTNGQ, F1IUBWX01EE9P4, F1IUBWX02HVNOG, CC F1GITI002IMJJO CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874252|FR874252.1:132991206-132991837. XX FH Key Location/Qualifiers FH FT source 1..626 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 626 BP; 187 A; 121 C; 124 G; 194 T; 0 other; cttaccaaaa cttatgctaa atgtactttc cagtatgaat gcacaagaga gtcccatcag 60 caggcagcat cggagtctag gagctcagct gtgtgtccat caacacacac attcgtaaaa 120 aacacatggc ctcgccatcg tgggtgaaat cggccccaca gcacgtctgc actggcgggc 180 gttactcccg tgctattctt ctgtgtaata ttaagaactg aatgtgaagt ttatagctag 240 cctgggtgta ccttttaaga attttgtaaa ctgtttgtct gtcttttgtt actgttttat 300 ggtgccaagt attctacatt acaacaataa tatcatggga gaaatagaaa tagcctagtt 360 tgcttccaat agaaattgct tttaacatgg gctgtatata aaaatattaa agagaaaact 420 gtacatttcc tcattgctcc gctacaaaca acccatgtaa taaccttgtc gcaaatattt 480 ttctcctagc actaagtaca gcattaaaag gtgattagag agtctgttga tgaaacacaa 540 atatacgttt tattgatttt actttagaac actacagagt tcctggaccg ggtgaaggca 600 ttaggtgggt gtttgttttt ggataa 626 // ID JK123697; SV 1; linear; mRNA; EST; MAM; 663 BP. XX AC JK123697; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO22FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-663 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 479151b0fc58a5c87dc69fc43894315d. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3630 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000009565 (IL27RA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000011557 CC High quality match with Homo sapiens transcript: ENST00000263379 CC (IL27RA-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000019009 (XM_001111176.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000017029 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000002461 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000019505 (XM_512969.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000011219 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX02FUIV8, F0BWDJC02GC7M8, F0M2C4T02HYN4E, F0DRJ6O02HICK3, CC F1HESOY01CB1S8 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/). XX FH Key Location/Qualifiers FH FT source 1..663 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 663 BP; 145 A; 190 C; 192 G; 136 T; 0 other; gacttgaact gctcgtggga gcctcttggg gacctgggag ccccctccga gttacacttt 60 cagagccaaa agtaccattc caacaaaacc cagaccatgg cagtggcagc cgggcagagc 120 tgggtggcca ttcctcggga acagctcacc atgtctgaca agctccttgt ctggggcact 180 aaggcaggcc agcctctctg gacccccatc ttcgtgaacc tggaaaccca aatgaagcca 240 aatgcccccc ggctgggccc tgacgtggac gtttccgagg atgagcccct ggaggccatt 300 gtccattggg tcccacctac atggccgtct cataaagttc tgatctgcca gttccactac 360 cgaagatgtc aggaggcagc ctggaccctg ctggaactgg agctgaagac catacccctg 420 gcccctgttg agatccaaga tttggagcta gccactggct accaagtgta tggccgctgt 480 cgaatggaga aagaagagga tttgtggggc gagtggagcc ccattttgtc cttccagaca 540 ccgccttctg ctccaaaaga tgtgtgggta tcagggaacc tctgtgggat gcctggagga 600 gaggaaccct tgcttctgtg gaaggcccca gggccctgtg tgcaggtgag ctacaaagtc 660 tgg 663 // ID JK123698; SV 1; linear; mRNA; EST; MAM; 458 BP. XX AC JK123698; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO23FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-458 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 64573384d05104206d22be8308be000d. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3476 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000020782 (LOC100392923) CC High quality match with Homo sapiens transcript: ENST00000215754 CC (MIF-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000009084 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK01BT6Q8, F0ZWYBK01BOT4D, F0Q2XTE01CR5W3, F1RHTUQ01DBRRG, CC F0X5I7102GYVCO CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/). XX FH Key Location/Qualifiers FH FT source 1..458 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 458 BP; 89 A; 167 C; 132 G; 70 T; 0 other; gccgatgttc atcgtaaaca ccaacgtgcc ccgcgcctca gtgccggacg ggttcctctc 60 cgagctcacc cagcagctgg cgcaggccac cggcaagccc ccccagtaca tcgcggtgca 120 cgtggtcccg gaccagctca tggccttcgg aggctccagc gagccttgcg cgctctgcag 180 cctgcacagc attggaaaga tcggcggcgc gcagaaccgc tcctacagca agctgctgtg 240 cggcctgctg gccgagcgcc tgcgcatcag cccagacagg gtctacatca actattacga 300 catgaacgcg gccaacgtgg gctggaacaa ctccaccttc gcgtaagagc cgccgggccc 360 cacgcggtct gcgctggctg gacccgggag cccgccgcac gcagtgttct aggctcgccc 420 accccaacct tctggagaaa taaaacggtt tagagacc 458 // ID JK123699; SV 1; linear; mRNA; EST; MAM; 613 BP. XX AC JK123699; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YO24FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-613 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 59a7304284af5dbe977a70e6f9d0358f. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3631 CC High quality match with Homo sapiens transcript: ENST00000247655 CC (COX7C-001) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Q2XTE01CZJP4, F0Q2XTE01EC673, F0Z8TLB02H4Q25, F0Z8TLB02F9NGE, CC FZ8FYPF02I8MGL CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:82721573-82722163. XX FH Key Location/Qualifiers FH FT source 1..613 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 613 BP; 167 A; 117 C; 136 G; 193 T; 0 other; catttcttga gcacgggaga acaaggtcgt gaaaacaagg tcttggtgag gtgccgccat 60 ttcatccgtc ctcggtctct gcgcctttcg cagagcttcc agcagcgcta tgttgggcca 120 cagcatccgg aggttcacaa cctctgtggt ccgtaggagc cactatgagg agggccctgg 180 gaagaatttg ccattttcag tggaaaacaa gtgggcgttg ctagttaaga tgtgtttgta 240 ctttggatct gcatttgctg cacccttcct tatagtaaga caccaactgc ttaaacaata 300 aggatgtttc agttcgtcca tttaccagat atgaagagca ttttaagaga tacagcctct 360 ggaaatggat caaactctaa ctcatggcat gctagatata tttgtcaata aacttatgac 420 atgaatgctt aattcctctt ttttgaaata gggaatgtaa tacttggcca tttgcctact 480 ttattgggta acattccagt attactctga gatttagctt atttaatggt gttaaactga 540 ggttatatta aatttttgat tcccaggtca ggattttgtt ggtaatttat gcaataaaag 600 ggaaatacaa atc 613 // ID JK123700; SV 1; linear; mRNA; EST; MAM; 639 BP. XX AC JK123700; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP01FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-639 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; a348ac49bc1dcfb796ae2b4a0d5007ea. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0SMK5U02IDO5E, F0DRJ6O02HUY6W, F0BWDJC02FODDS, F0X19WM01CUBYV, CC F0DTF6N02HZF32 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/). XX FH Key Location/Qualifiers FH FT source 1..639 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 639 BP; 127 A; 142 C; 220 G; 121 T; 29 other; gggctcacca tgaagccagg tttcagtccc cgtgggggtg gctttggcgg ccgagggggc 60 tttggtgacc gtggtggtcg tggaggccga gggggctttg gcgggggccg aggtcgaggc 120 ggaggcttta gaggtcgtgg acgaggannn nnnnnnnnnn nnnnnnnnnn nnnnnnagga 180 ggaggaagag gtggaggctt ccattctggc ggcaaccggg gtcgtggtcg gggaggaaaa 240 agaggaaacc agtcggggaa gaatgtgatg gtggagccgc atcggcatga gggtgtcttc 300 atttgtcgag gaaaggaaga tgcactggtc accaaaaacc tggtccctgg ggaatcagtt 360 tatggggaga agagagtctc gatttcggaa ggagatgaca aaatcgagta ccgagcctgg 420 aaccctttcc gctccaagct ggctgcagca atcctgggtg gtgtggacca gatccacatc 480 aaaccggggg ctaaggtgct ctacctcggg gctgcctcgg gcaccaccgt ctcccatgtc 540 tctgacatcg tcggtccgga tggtctagtc tatgcagtcg agttctccca ccgctctggc 600 cgtgacctca ttaacttggc caagaagagg accaacatt 639 // ID JK123701; SV 1; linear; mRNA; EST; MAM; 754 BP. XX AC JK123701; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP02FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-754 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 88181535c6ef93568850cd1abe7f11c9. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2199 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000041057 (TUBA1A) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000022390 CC High quality match with Homo sapiens transcript: ENST00000301071 CC (TUBA1A-202) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000013672 (Q9GLW6_MACMU) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000017551 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000022764 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000013491 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000023990 (XM_509042.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000005317 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000013258 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T02H8IRQ, FZ8A1BE02GWTGZ, F1RHTUQ01ATTJY, F1GITI002FQB6W, CC F1HESOY02I0OST CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874249|FR874249.1:43422409-43421761. XX FH Key Location/Qualifiers FH FT source 1..754 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 754 BP; 165 A; 221 C; 190 G; 178 T; 0 other; atcgaccgcc taagagtcgc gctgtaagaa gcaacaacct tctcctcctc gtctccgcca 60 tctgctcggc agtcgcgaag cagcaaccat gcgtgagtgc atctccatcc atgttggcca 120 ggctggtgtc cagattggca atgcctgctg ggagctctac tgcctggaac atggcatcca 180 gcccgatggt cagatgccaa gtgacaagac cattggggga ggagatgatt ccttcaacac 240 cttcttcagt gagactggcg ctggcaagca tgtgccccgg gccgtgtttg tagacttgga 300 acccacggtc attgatgaag ttcgcactgg cacctaccgc cagctcttcc accctgagca 360 gctcatcaca ggcaaggaag atgctgccaa taactatgcc cgagggcact acaccattgg 420 caaggagatc attgaccttg tgttggaccg aattcgtaag ctggctgacc agtgcaccgg 480 tcttcagggc ttcttggttt tccacagctt tggtggggga actggttctg ggttcacctc 540 cctgctcatg gaacgtctct cagttgatta tggcaagaag tccaagctgg agttctccat 600 ttacccagcg ccccaggttt ccacagctgt agttgagccc tacaactcca tcctcaccac 660 ccacaccacc ctggagcact ctgattgtgc cttcatggta gacaatgagg ccatctatga 720 catctgtcgt agaaaccttg atattgagcg tcca 754 // ID JK123702; SV 1; linear; mRNA; EST; MAM; 740 BP. XX AC JK123702; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP03FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-740 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1e6a9f290d5215296a56a2e820b7e5a8. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000034577 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000006566 CC High quality match with Homo sapiens transcript: ENST00000370059 CC (KCNIP2-012) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000022151 (XM_001111683.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000049530 (XM_001170544.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000003117 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01ANIJL, F0M2C4T01AFBGN, F1IUBWX01AW0YF, F1RTSFR02F2P5L, CC F01W12W01BX9Z6 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874252|FR874252.1:101937361-101936621. XX FH Key Location/Qualifiers FH FT source 1..740 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 740 BP; 162 A; 196 C; 186 G; 196 T; 0 other; ccagtgttga gagtgcccct cctgtaggaa ctgagtggtt ccccacctcc tacccccact 60 ctagaaacac actagacaga tgtctcctgc tatggtgctt cccccatccc tgacttcata 120 aacatttccc ctaaaactcc cttctcagag agaatgctcc attcttggca ctggctggct 180 tctcagacca gcctttgaga gccctgtggg agggggacaa gaatgtatag ggagaaatct 240 tgggcctgag tcaatggata ggtcctagga ggtggctggg gttgagaata gaaaggcctg 300 gacacaatgt gattgctcag gcataccagg ttatagctcc aagttccaca ggtctgctac 360 cacaggccat caaaatataa gtttccaggc tttgcagaag accttgtctc cttggaaatg 420 ccccagaaac tttccatacc ctcctcgata tccatggaga gcctggggct agatatctgg 480 catatccctg gcattgcttc ctctccttcc ttcctgcatg tgttggtggt ggttgtggca 540 ggggaatgtg gatagcagat gtcctggctg atgcctgcca aagtttcatc ccaccctccc 600 tgctcatcgt ccctgttttg agggctatga cttgagtttt tttttcccat gttctctata 660 gacttgggac cttcctgaac ttggggccta tcactcccca cagtggatgc cttagaaggg 720 agagggaagg agggaggcag 740 // ID JK123703; SV 1; linear; mRNA; EST; MAM; 746 BP. XX AC JK123703; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP04FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-746 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 3a859237da9a9bec838a09fbefbbdb41. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3434 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000038695 (A6MKP8_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000011647 CC High quality match with Homo sapiens transcript: ENST00000263867 CC (CAPG-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000043396 (CAPG) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000015062 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000002216 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000022550 (XM_515584.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000014195 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z1IVZ02HOGZM, F01W12W02IDRE8, F0Q2XTE01EA01V, F0ZWYBK01C06AA, CC F01W12W01B8BO2 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874256|FR874256.1:86381031-86380961. XX FH Key Location/Qualifiers FH FT source 1..746 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 746 BP; 180 A; 199 C; 244 G; 123 T; 0 other; gtgagaagcc gggtggggca ggctggaagg aagacgaacc tacgaagcag aagtaggagc 60 aagaaaggaa gatctgaaga cagcatgtac acagccattc cccagagtgg ctctccattc 120 ccaggctcag tgcaggatcc aggcctgcat gtgtggcggg tggagaagct gaagccagtg 180 cccgtggcac gagagaacca gggcatcttc ttctcggggg actcctacct agtgctgcac 240 aatggcccag aagaggtttc ccatctgcac ctgtggatag gtcagcagtc atcccgggat 300 gagcaggggg cctgtgccgt gctggctgtg cacctcaaca cgctgctggg agagcggcct 360 gtgcagcacc gcgaggtgca gggcaatgag tccgacctct tcatgagcta cttcccacga 420 ggcctcaagt accaggaagg tggtgtggag tcagcatttc acaagacctc cacaggagcc 480 ccagctgcca tcaagaaact ctaccaggtg aaggggaaga agaacatccg tgccaccgag 540 cgggcactga actgggacag cttcaacact ggggactgct tcatcctgga cctgggccag 600 aacatcttcg cctggtgtgg tggaaagtcc aacatcctgg aacgcaacaa ggcgagggac 660 ctggccctgg ccatccggga cagtgagcga cagggcaagg cccaggtgga gattgtcact 720 gatgggggag gagcctgctg agatga 746 // ID JK123704; SV 1; linear; mRNA; EST; MAM; 565 BP. XX AC JK123704; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP05FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-565 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2c16929bbc92622bbfb812f728f48fcc. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE01C948D, FZ8A1BE01A4ARO, F1GITI002H4SBQ, F0Q2XTE02FKGW7, CC FZ8A1BE02JGLTM CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:135036075-135036038. XX FH Key Location/Qualifiers FH FT source 1..565 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 565 BP; 136 A; 111 C; 137 G; 132 T; 49 other; ccccggttac tctgaagaaa gcagaggcta tggaagtggt ggagaaggtt gtagaaacca 60 gggcagtggc ttctgtgaca gctataacaa cggagacaga ggcggctttg gcagtggtag 120 tagaaacaat tgcagaagta gtagaagata caatgatttt ggcaagtaca acagtcagtc 180 ttcagatttt gaacccatga aggcaggaaa ctttgaaggc agaaactctg acccctatcg 240 tggtggaggc caatattttg ccaaaccacg aaaccaaggt ggctatggtg gttctagtag 300 caacagtagc tacggccatt gctactgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 360 nnnnnnnnnn nnnnnngaga tggattcttg cactgttgcc tccaggctgg agtgcggtgg 420 tgtgatcttg gctcactgca acctctgcct cctgggttca cacaattttc atgcctcagc 480 ctcctaagta gctgggatta caggtgccca ccaccacacc cagctaattt tttgtatttt 540 tagtagagat aggtttcact ctgtt 565 // ID JK123705; SV 1; linear; mRNA; EST; MAM; 75 BP. XX AC JK123705; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP06FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-75 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f8aca48113d6a1ac4391f0be1ebdf6bd. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000057387 (SNRK) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000014177 CC High quality match with Homo sapiens transcript: ENST00000429705 CC (SNRK-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000001466 (XM_001115106.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000007498 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000027683 (XM_001142896.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01EXZTM, F0DTF6N02HFAL4 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874245|FR874245.1:93544042-93543968. XX FH Key Location/Qualifiers FH FT source 1..75 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 75 BP; 30 A; 9 C; 16 G; 20 T; 0 other; cattgtgtaa acattgacag gtatgtgaca aatgggaaaa aatccaaata ataaagtgac 60 atattggtgt tcagc 75 // ID JK123706; SV 1; linear; mRNA; EST; MAM; 754 BP. XX AC JK123706; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP07FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-754 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; c5e2c7d27051c33481fc38c37d920f5b. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3632 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000040281 (LOC100415390) CC High quality match with Homo sapiens transcript: ENST00000356357 CC (KIAA0317-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000012421 (XM_001094796.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000020772 CC High quality match with Pongo abelii transcript: ENSPPYT00000007078 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X5I7101E2QYO, F0X5I7101B2NES, F0X19WM02FZT67, F1RTSFR01DXEXC, CC F0DTF6N01E1X8T CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:138633574-138632824. XX FH Key Location/Qualifiers FH FT source 1..754 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 754 BP; 158 A; 204 C; 179 G; 213 T; 0 other; tgacttccca aatttgtgtt tttaagaagg ctttgctgca agctatcatt cccaatgggg 60 cagtcaactc tgagaatatt acaagccatc cttattgcca aaaccagcaa gtacacagag 120 tcctgagaca aggcaggact ttggcaatgc cttggcctcc tggaagcatg tctgagccct 180 cctgttccca gggatcatga ggaacaagcc ctcctgagct gctacagttc ctgcctgacc 240 tcactctttc gcagtcatca tttgtccttt cttggcacat gggtgctgac ctcactcatc 300 cttggggtct gggatccagc atcagggtct ctgtacccca ggatcctcca tgccacatgg 360 tcactgctct cctcagggac caggacaagg tgctgctgct tgcccaaatg ttttcccatg 420 ggatatgtac aggaaggttt atcatcaccc tgggaaacat gatgctgccc cttgggccca 480 gaaaggggtt tccagctggg ggcacgtgga ctggttctgc tgtttttggc agttgctttt 540 ctgaattctc ccctccggca gccttccaga gactgatcct ggagactgag ttgattgtct 600 tggctagatc tgtcatttta agctctagtc atagcacttt ttcagagcat cttaggcacc 660 attgcaaccc aaccagggca gctgcatccc tgtggtggtc cttaggcacc agtctttgtt 720 aaacaaaaac cctttggtac tgttgtgggt ttct 754 // ID JK123707; SV 1; linear; mRNA; EST; MAM; 424 BP. XX AC JK123707; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP08FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-424 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2d368d74a29cca7b1f8878dd4ea7a9fc. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig579 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000018986 (LOC100405119) CC High quality match with Homo sapiens transcript: ENST00000233143 CC (TMSB10-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000004870 (XM_001083000.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000058822 (XM_001165388.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF02JFHCY, FZOOKC302IYVSQ, F0X19WM01BHH96, F1C5L3D03F4H6C, CC F1HESOY01DFJGT CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874261|FR874261.1:41192581-41192146. XX FH Key Location/Qualifiers FH FT source 1..424 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 424 BP; 131 A; 110 C; 107 G; 76 T; 0 other; cccgggctcg gaacccgact acacggattg ttttaagaaa atggcagaca aaccagacat 60 gggggaaatc gccagcttcg ataaggccaa gctgaagaaa acggagacgc aggagaagaa 120 caccctgccg accaaagaga ccattgagca ggagaagcgg agtgaaattt cctaaaatcc 180 tggaggattt cctacccccg tcatcttcga gaccccagtc gtgatgtgga ggaagagcca 240 cctgcaagat ggacacgagc cacaagctgc actgtgaacc tgggcactcc gcgccaatgc 300 caccggcctg tgggtctctg aagggacccc cccaatcgga ctgccaaatt ctccggtttg 360 ccctggaata ttatagaaaa ttatttgtat gaataatgaa aataaaacac acttcgtggc 420 aaaa 424 // ID JK123708; SV 1; linear; mRNA; EST; MAM; 703 BP. XX AC JK123708; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP09FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-703 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 8f5a32f149175c1e6f86eece3bda7633. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000031426 (LOC100398762) CC High quality match with Homo sapiens transcript: ENST00000374518 CC (BMS1-001) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000020137 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000004533 (XM_521453.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF02F1KSQ, F1C5L3D02C5J8P, F1RHTUQ02F6NDM, F0DTF6N01EBREX, CC F0DTF6N01BTYYD CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874252|FR874252.1:45695189-45695070. XX FH Key Location/Qualifiers FH FT source 1..703 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 703 BP; 215 A; 158 C; 174 G; 156 T; 0 other; attttcggat agctgccaca ggagttgtcc ttgatctgga taaatccata aaaattgtga 60 agaaattaaa gctaactggt tttccatata aaattttcaa gaatacttca tttattaagg 120 gaatgtttaa ttctgccttg gaagtggcca aatttgaagg tgctgtgatt cgaactgtca 180 gtgggataag ggggcagatc aagaaagcac tccgagctcc agaaggcgct ttccgggcca 240 gctttgagga taaactgctg atgagtgata ttgtcttcat gcgaacttgg tatcctgttt 300 ccatcccagc cttctataac ccagtaacat ctttgttgaa accagtgggt gagaaagaca 360 cctggtcagg aatgcggacc acaggccaac tcaggctcgc ccatggcgtc agactaaagg 420 caaacaagga ctctctgtat aagccaatcc tgaggcaaaa gaaacatttt aattcactgc 480 acattccaaa agccttgcag aaggccctgc catttaagaa caagcccaag acccaagcaa 540 aggcaggcaa ggtgccaaag gacaggcgga gaccggccgt catacgggag cctcatgaaa 600 gaaagatcct cgcgctgctg gatgctctga gtacggtgca cagtcagaag atgaagaagg 660 ccaaggagca gcggcacctg cacaataagg agcacttcag agc 703 // ID JK123709; SV 1; linear; mRNA; EST; MAM; 702 BP. XX AC JK123709; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP10FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-702 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 3e69bd5e6007d022da940750578fe092. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB01DTYLJ, FZ8FYPF01CIIK2, F0Z8TLB01EEB4B, F1C5L3D03G4H20, CC F1IUBWX01ESSUH CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874246|FR874246.1:143967184-143967033. XX FH Key Location/Qualifiers FH FT source 1..702 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 702 BP; 225 A; 92 C; 109 G; 245 T; 31 other; gtcagttatt agtgacttaa agggagaaca gaggtgtttt ttatgtgtga tactggtcat 60 tcatatgtat gagaaaacac atctgctaat tattgtgaag ttactttaaa gggaaggtta 120 tttaaatatg aaggattatt aaataagatt acgnnnnnnn nnnnnnnnnn nnnnnnnnnn 180 nnnngctgct ggatcataag aatggaaatg gtaagtaagt aatacaacag tcttttatca 240 ttcagattta ttctgtttcg tatgtcatag gagacataag aaaactagag gtttgcataa 300 aatacttctc ggttgttaaa aagataagat gtcaactgtt ttaatttgca gtgcttatcc 360 aacatacatg ttaggaatct atgttttctg cagacttttc aaatcttatt gaaaagggaa 420 ctagaatgat cttatagata attatatttc attaaaattc tttataacta gaagctattt 480 ctcgccattt tgtcattatc ccaacaaatt tttttactaa atctatcaat gtgcccttta 540 tttctcatat tttgtctagt aaacttttaa aaatactaca cttcttaaag cagcattcag 600 acctgttttt gtaggtggtt tttctgtctg ataaaactat aaaactgacc tagttgtatc 660 attcagaaaa gtaaaaagta attttccacc tcggaatgac ca 702 // ID JK123710; SV 1; linear; mRNA; EST; MAM; 596 BP. XX AC JK123710; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP12FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-596 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 3ce553afa511c2d14c7fd81307d3acdd. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0SMK5U02FJUXE, FZ8FYPF02JLSZP, FZOOKC302IL7OY, FZ8FYPF02JZ74G, CC F0M2C4T01CLJJE CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874253|FR874253.1:68986275-68985689. XX FH Key Location/Qualifiers FH FT source 1..596 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 596 BP; 125 A; 158 C; 168 G; 145 T; 0 other; ctccctcagc cccagccctt cttcagtccc aggcctccct cagtcccagc cctccctcag 60 cgccagacct tcctcagtct taagagtggg gatgggcaaa gctggcacaa gctcctgctg 120 tccagggtgc taagtgctct cagagagtta tgagtaagga gagtgtgctc cctgggctgt 180 agagaggagg agtcccccag cagttggagg caaggaaggg cattccatgc aggaagttca 240 cagcagtggg caactgcggg gcttatcggg gaacagcact ctgtcctttc ttgctgaagc 300 aggacatgag tagggaggag catcccgtga tgggcctgtg cagagccaag gagtttgaac 360 tttatccaac tggtaccatg aggaacgtca gcccgggggc ttccagctca cctggaattt 420 ttgccctttg gagccttgca aaggcttggg atgttagaaa ggctcaggtt gaggtttctc 480 agcccagaat tctcctgccc tgaccttgag tcttatttgc atcttgctcc cgtaagtgtt 540 ctggtctacc cttcagagaa tgaacagtgt ttactgcaag ggctttattt tccaga 596 // ID JK123711; SV 1; linear; mRNA; EST; MAM; 694 BP. XX AC JK123711; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP13FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-694 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 25cbbf79b1125da1720c2f7dc9f703a0. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2235 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000009594 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000033888 CC High quality match with Homo sapiens transcript: ENST00000234831 CC (TMEM59-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000047102 (XM_001113367.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000001447 (XM_001150793.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000001589 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE02HM4WR, FZ8A1BE02G4N04, F1HESOY01A70VC, FZ8A1BE01AUK18, CC F0BWDJC01BI5TM CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:57224736-57224542. XX FH Key Location/Qualifiers FH FT source 1..694 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 694 BP; 185 A; 159 C; 170 G; 180 T; 0 other; acaagatggc ggcgccgaag gcgatcctct gggtcaggac ccaactgggg ctgccgccgc 60 tgctgctgct gaccatggcc ttggccggag gttcggggac cgcttcggct gaagcatttg 120 actcggtctt gggtgataca gcgtcttgcc accgggcctg tcagttgacc taccccttgc 180 acacctaccc taaggaagag gagttgtacg catgtcagag aggttgcagg ctgttttcaa 240 tttgtcagtt tgtggatgat ggaattgact taaatcgaac taaattggaa tgtgaatctg 300 catgtacaga agcatattcc caatctgatg agcaatatgc ttgccatctt ggttgccaga 360 atcagctgcc attcgctgaa ctgagacaag aacaacttat gtccctgatg ccaaaaatgc 420 acctactctt tcctctaact ctggtgaggt cattctggag tgacatgatg gactctgcac 480 agagcttcat aacctcttca tggacttttt atcttcaagc tgatgatgga aaaatagtca 540 tattccagtc taagccagaa atccagtatg caccacattt gggacaggag tctacaaatt 600 tgagagaatc atctctaagc aaaatgtcct cagatctgca aatgagaaat tcacaagcac 660 acaggaattt tcttgaagat ggagaaagtg atgg 694 // ID JK123712; SV 1; linear; mRNA; EST; MAM; 647 BP. XX AC JK123712; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP14FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-647 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 26633a900f58eccd82a5a1a7a7acb9a4. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1050 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000042080 (D7UTH3_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000001311 CC High quality match with Homo sapiens transcript: ENST00000339647 CC (UBC-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000045385 (LOC705140) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000005416 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000003226 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000005667 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000061629 (UBIQ_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000006033 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB02IY2DJ, F0BOF8H01EOIID, F0X19WM02I8SB6, F0X19WM01AJSSA, CC F0Z8TLB01CG0I1 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:126777459-126777420. XX FH Key Location/Qualifiers FH FT source 1..647 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 647 BP; 175 A; 177 C; 173 G; 122 T; 0 other; ggatcgcagt tcttgtttgt gtgtcggtgt catcgccact tgacaatgca gatcttcgtg 60 aagactctga ctggcaagac catcaccctt gaggttgagc ccagtgacac catcgaaaat 120 gtcaaggcaa agatccagga caaggaaggc attcctcccg accagcagag gctgatcttt 180 gccggaaagc agctggaaga tgggcgcacc ctgtctgact acaacatcca gaaagagtcc 240 accctgcact tggtgctccg cctcagaggt gggatgcaga tcttcgtgaa gaccctgact 300 ggcaagacca tcaccctcga ggtggagccc agtgacacca tcgagaacgt caaagcaaag 360 atccaggata aggaaggcat tcctcctgac cagcagaggt tgatcttcgc tggaaagcag 420 ctggaagatg ggcgcaccct gtctgactac aacatccaga aagagtccac cctgcacctg 480 gtgctccgcc tcagaggtgg gatgcagatc ttcgtgaaga ccctgactgg caagaccatc 540 accctcgagg tggagcccag tgacaccatc gagaatgtca aggcaaagat ccaggataag 600 gaaggcatcc ctcctgacca acagaggttg atctttgctg gaaaaca 647 // ID JK123713; SV 1; linear; mRNA; EST; MAM; 442 BP. XX AC JK123713; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP15FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-442 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 58076d567245a25c19b076f01948b40c. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig846 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000013096 (LOC100413255) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000004131 CC High quality match with Homo sapiens transcript: ENST00000348462 CC (RPL35-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000046654 (XM_001082318.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000002918 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000022068 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000008516 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000039513 (XR_023153.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000022874 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z1IVZ01BUP10, F0DRJ6O01D1HQT, F0Z8TLB01E2T9C, FZ8A1BE02FFXJ5, CC FZ8A1BE01CRJ3A CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874248|FR874248.1:138080223-138080611. XX FH Key Location/Qualifiers FH FT source 1..442 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 442 BP; 120 A; 122 C; 137 G; 63 T; 0 other; cgggcggcgg cggctgcatc ggcggcctgt gtagcaatgg ccaagatcaa ggctcgagat 60 cttcgcggga agaagaagga ggagctgctg aaacagctgg acgacctgaa ggtggaactg 120 tcccagctgc gcgtcgccaa agtgacgggc ggtgcggcct ccaagctctc taagatacga 180 gtcgtccgca aatccatcgc ccgtgttctc acagtcatta accagactca gaaagaaaac 240 ctcaggaaat tctacaaggg caagaagtac aagcccctgg acctgcggcc taagaagaca 300 cgcgccatgc gccgccggct caacaaacac gaggagaacc tgaagaccaa gaagcagcag 360 cggaaggagc ggctataccc gctgcggaag tacgcggtca aggcctgagt ggcgcgttgt 420 caataaagca cagctggctg ag 442 // ID JK123714; SV 1; linear; mRNA; EST; MAM; 608 BP. XX AC JK123714; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP16FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-608 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 225e1be5280ecd936b6ddad34d6585c1. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3323 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000007169 (A6MJY8_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000000507 CC High quality match with Homo sapiens transcript: ENST00000534676 CC (PRMT1-017) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000004290 (XM_001113609.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000012878 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000006738 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000011940 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000066946 CC High quality match with Pongo abelii transcript: ENSPPYT00000004928 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000019397 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RTSFR02J3B82, FZ8A1BE01EEQVN, F1RHTUQ01AT4EL, F1HESOY02I4XVT, CC F01W12W02JF1RC CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874249|FR874249.1:174826416-174826284. XX FH Key Location/Qualifiers FH FT source 1..608 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 608 BP; 140 A; 166 C; 185 G; 117 T; 0 other; gtagttgcgg gtgaagatgg cggcagccga ggccgcgaac tgcatcatgg aggtgtcctg 60 tggccaggcg gaaagcagcg agaagcccaa tgctgaggac atgacatcca aagattacta 120 ctttgactcc tacgcccact ttggcatcca cgaggagatg ttgaaggacg aggtgcgtac 180 cctcacatac cgcaactcca tgtttcacaa ccggcacctc ttcaaggaca aggtggtgct 240 ggacgtcggc tcgggcaccg gcatcctctg catgttcgcc gccaaggccg gggcccgcaa 300 ggtcattggg atcgagtgtt ccagtatctc tgattatgcg gtgaagatcg tcaaagccaa 360 caagttagac cacgtggtga ccatcatcaa ggggaaggtg gaggaggtag agctcccggt 420 ggagaaggtg gacatcatca tcagcgagtg gatgggctac tgcctcttct acgagtccat 480 gctgaacacc gtgctctacg cccgggacaa gtggctggcg cccgatggcc tcatcttccc 540 agaccgggcc ccgctgtatg tgacggccat cgaggatcgg cagtacaaag actacaagat 600 ccactggt 608 // ID JK123715; SV 1; linear; mRNA; EST; MAM; 670 BP. XX AC JK123715; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP17FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-670 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 421e77b3db0363bea316ea9c7b9fa795. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2310 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB01ES36U, F0Z8TLB01EHTQE, F0ZWYBK01DHQ4E, F0ZWYBK01C949T, CC F0ZWYBK01CC0ZV CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:134562111-134561445. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 670 BP; 138 A; 178 C; 158 G; 196 T; 0 other; agaggttcat cctcttcctc ctctgctcct agaagcgaga tgagcgcagt gtgaagagca 60 cagctgtgcg ggtggtagac tttggcagtg ccacctttga ccatgagcac catagcacca 120 ttgtctccac tcgccattac cgagcaccag aggtcatcct tggtaaggga gggcaaggct 180 gtccaagtgt gtgagatgat gtggtgaggg tggggccacc taagcctcat aacacctttt 240 ccctcccatt ctcacccaga gttgggctgg tcacagcctt gtgatgtatg gagtataggc 300 tgcatcatct ttgagtacta tgtgggattc accctcttcc aggtaagtga tgggatatct 360 tacttgacca cctggcatta ttctacctgg ttgctttgtt gtctgctgag gacctgccta 420 ttcagttctc cacattgccc accttcctgg cagctggtcc tcagcatgcc cttttcagtc 480 ccatgctcag ttctgttttt gtttcccaga cccatgacaa cagagagcat ctagccatga 540 tggaaaggat attgggtcct atcccttccc ggatgatccg aaagacaagg tgaaccttga 600 gtgggcacta gttaaccctt ttccttttct ctccacagaa ttggtctatt tcacatcagt 660 ttcttttttc 670 // ID JK123716; SV 1; linear; mRNA; EST; MAM; 706 BP. XX AC JK123716; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP18FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-706 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; e477e935641deb906487ef236f5d2896. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1180 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000019570 (B0KWA9_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000023460 CC High quality match with Homo sapiens transcript: ENST00000309268 CC (EEF1A1-009) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000015401 (Q3YAI9_MACMU) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000015309 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000005760 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000011455 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000048233 (EF1A1_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000019520 CC (EF1A1_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0OTZ2I02GIIWK, F1RHTUQ02HVYYQ, F1HESOY01A5BGV, F0BOF8H01EMS2I, CC F1RHTUQ02IXMRF CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874251|FR874251.1:55790189-55789564. XX FH Key Location/Qualifiers FH FT source 1..706 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 706 BP; 212 A; 147 C; 179 G; 168 T; 0 other; gtttgccgcc agaacacagg tgtcgtgaaa actaccccta aaagccaaaa tgggaaagga 60 gaagactcat atcaacattg tcgtcattgg acacgtagat tcgggcaagt ccaccactac 120 tggccatctg atctacaaat gcggtggcat cgacaaaaga accattgaaa aatttgagaa 180 ggaggctgct gagatgggaa agggctcctt caagtatgcc tgggtcttgg ataaactgaa 240 agctgagcgg gaacgtggta tcaccattga tatctccttg tggaaatttg agaccagcaa 300 gtattatgtg actatcattg atgccccagg acacagagac ttcatcaaaa acatgattac 360 agggacatct caggctgact gtgctgtcct gattgttgct gctggtgttg gtgaatttga 420 agctggtatc tccaagaatg ggcagacccg agagcatgct cttctggctt acacactggg 480 tgtgaaacaa ctaattgttg gtgttaacaa aatggattcc actgagccac cctacagcca 540 gaagagatac gaggaaattg ttaaggaagt cagcacttac attaagaaaa ttggctacaa 600 ccccgacaca gtagcatttg tgccaatttc tggttggaat ggtgacaaca tgctggagcc 660 aagtgctaac atgccttggt tcaagggatg gaaggtcacc cgtaag 706 // ID JK123717; SV 1; linear; mRNA; EST; MAM; 721 BP. XX AC JK123717; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP19FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-721 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 0db2bec42fcb904c05fd637370ab27df. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Homo sapiens transcript: ENST00000495780 CC (CR933844.2-002) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0SMK5U02GVVFH, F1GITI002F6GSZ, FZOOKC302FTX7I, F0Q2XTE02GF1XW, CC F1RTSFR02I4JLH CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:157041881-157041840. XX FH Key Location/Qualifiers FH FT source 1..721 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 721 BP; 210 A; 139 C; 200 G; 172 T; 0 other; ctgcagtttg agctattgca gctatagttt tcagggttcg tttagcaggg atgatggtaa 60 cagtatagga aagtgagact taaaattcta tcaaccttta ttccgaatat ttccttcagg 120 atagaactgg aggaacctcc acttgttctg gctgctgcaa atgtggtgag aaatatcacc 180 tacaaatatc gagaggactt gtctgcacat ctcatggtag ctggctggga ccaacgtgaa 240 gggggtcagg tatatggaac cctgggagga atgctgactc gacagccttt tgccattggt 300 ggctccggca gcacctatat ctacggttat gtggatgcag catataagcc aggcatgtct 360 ccagaggagt gcaggcgctt caccacagac ggtaaccagc caagtggaag ggtacctggg 420 gagggctttt aaacatggga aggaagtaga ttaccaggaa caggaataga aatacagggg 480 tgaccattta agttaatgcc caaactagta cacttttaaa agtgaaaagg ggcaggacaa 540 atgcaaagct cagtggggct cttgggcaat atggataaac cagggctgtt ctgagtaaat 600 caaatgagga tccacagtca ctgtaagaac cagtggtgtg ctaagcacaa tggctcacac 660 ctgtaatgcc aatactttgg gaggctgagg caagaggatt acttgagccc aggagtttga 720 g 721 // ID JK123718; SV 1; linear; mRNA; EST; MAM; 638 BP. XX AC JK123718; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP20FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-638 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 67a992ad2850b6dfb50992e1cee9bf4f. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3633 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000053666 (LOC100408503) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000013677 (Q30494_9PRIM) CC High quality match with Homo sapiens transcript: ENST00000551599 CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000027204 (NP_001127765.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000008671 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000067980 (Q30926_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000020070 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000009754 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZOOKC302FJSFC, F0DRJ6O02FJ087, F1HESOY01AU18K, F0M2C4T01CXBDZ, CC F0OTZ2I01CX6IK CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:33079440-33079524. XX FH Key Location/Qualifiers FH FT source 1..638 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 638 BP; 149 A; 176 C; 192 G; 121 T; 0 other; gcagctcatt tcattttgcc atccttttcc agctccatga tggttctgca ggtttctggg 60 accccccaga cagtgggtct gatggcgtta ctgatggtgc tgctcacatc tgtggtccag 120 ggcagggcca ctccagagaa ttacctgtac cagggacggc aggaatgcta cgcgtttaat 180 gggacacagc gctacctgga gagatacatc tacaaccggg aggagtacgt gcgcttcgac 240 agcgacgtgg gggaattccg ggcagtgacg gagctggggc ggcctgatgc ggaatactgg 300 aacagccaga aggacatcct ggaggagaag cgggcagtgg cgggcacgag ttgcagacac 360 gactacgagc tggacgaggc cgtgaccctg cagcgccgag tccagccgag ggtgaacatc 420 gccccctcca agaagggtcc cttacagcac cacaacctgc tcatctgcca agtgacagat 480 ttctacccag gcagcattca agtccgatgg ttcctgaatg gacaggagga aacagctggg 540 gtcgtgtcca ccaacctgat ccgtaatgga gactggacct tccagatcct aatgatgctg 600 gaaatgaccc cccagcaggg agacgtctac acttgcca 638 // ID JK123719; SV 1; linear; mRNA; EST; MAM; 681 BP. XX AC JK123719; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP21FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-681 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 3fcf400f6ac5ce4d54e3fb4f856478d0. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB02JW878, F0X19WM02IWXN7, F0M2C4T02GQAXM, F0Q2XTE02FZTPI, CC F1C5L3D02D8JB6 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:108164013-108164693. XX FH Key Location/Qualifiers FH FT source 1..681 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 681 BP; 202 A; 110 C; 189 G; 180 T; 0 other; gaggagaact tctttagatg ttcagggaaa gtccctctga gcaggtgaaa tgttggacag 60 agccatgtga gtacctggtg ggagagtgtt tcaggcaaag gaaacagtaa gtcagaggcc 120 ccaagagagg aacaggcttg ctccatttga ggagtaatga agaggccagt gttgctggag 180 tagcgtgagt gtcgggacac gggaatagga atagtgaggt gtgcaggaac tggagtatgt 240 ctcaccatag ggccataata tagaatgttg gcagtacaga ttccgagtat gataggaagc 300 cattggtgag ttgaggacag aagaatgacc tattgtgatt tgcattttag aaggagtccc 360 ctgacttttt atgcaaagaa tagattgttg agggattaga gtggaagcag ggagattaag 420 tagaagattg tctgtggcag cccaagcaag aaaggatggt ggcctgacca gggaggtcac 480 cctgaaggag gaatcatccc atacacactg tttcccaacc tgtttaagtg tctatttctt 540 tgtaaaacta tttatctttg aatatagcta tggtactttt catattttta gaactagtct 600 tttcattcac tacagactga aaatcattct tatccagtca gtcataaatg aataaggacc 660 tgccagtgat gggtccaaag g 681 // ID JK123720; SV 1; linear; mRNA; EST; MAM; 704 BP. XX AC JK123720; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP22FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-704 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; ed2205be46745b24c7ac72b3a87d54c0. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2166 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000056675 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000027743 CC High quality match with Homo sapiens transcript: ENST00000550965 CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000029052 (XR_014486.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000007686 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000067995 (NM_001009081.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000019065 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000009707 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF01CFKIM, FZ8FYPF01DME5P, FZ8FYPF01CVWK7, F1RHTUQ01CSXEI, CC F0DTF6N01BMSNO CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:31172360-31172311. XX FH Key Location/Qualifiers FH FT source 1..704 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 704 BP; 146 A; 200 C; 208 G; 150 T; 0 other; gcgcgcggac cccccaaaga cacacgtgac ccatcacccc gtctctgacc atgaggccac 60 cctgaggtgc tgggccctgg gcttctaccc tgcggagatc acactgacct ggcagcggga 120 tggggaggac caaactcagg acacagagct tgtggagacc aggccaggag gagatggaac 180 cttccagaag tggggagctg tggtggtgcc ttctggagaa gagcagagat acacgtgcca 240 tgtgcagcat gatgggctgc cggagcccct caccctgaga tgggagccat cttcccagtc 300 caccatcccc atcgtgggca ttgtttctgg cctggctgtc ctagcagttg tggtcaccgg 360 agctgtggtc gctgctgtga tgtggaggag gaagagctca ggtggaaaag gagggagcta 420 ctctcaggct gcatccagcg acagtgccca gggctctgat gtgtctctca cagcttgaaa 480 agcctgagac aactaccttg tgtggaactg agaagcagga tttcttcacg cctccctttt 540 gaagagactc tggcatctct ttctgcaaag gcatctgaat gtgtctgcgt ccctgttagc 600 ataatgtgag gaggtggaga gacagcccat ccctgtgtcc accgtgaccc ctgttcccac 660 gctgaccagt gtttcctccc cagtcatctt tcctgttcca gaga 704 // ID JK123721; SV 1; linear; mRNA; EST; MAM; 693 BP. XX AC JK123721; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP23FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-693 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 218e18a3b7654f5140f0745eae729de6. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2044 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000017202 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000025682 CC High quality match with Homo sapiens transcript: ENST00000551150 CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000045510 (Q9GMG8_MACMU) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000012550 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000000394 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000003846 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000010142 (XR_020374.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000005944 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000009733 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02JU0AD, F0M2C4T02GUYJL, F0ZWYBK02J4EMX, F0Z8TLB02FY2ZN, CC F0BOF8H02HBL89 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:12557795-12557105. XX FH Key Location/Qualifiers FH FT source 1..693 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 693 BP; 170 A; 189 C; 186 G; 148 T; 0 other; aaaccctgcg tggcaatccc tgacgcaccg ccgtgatgcc cagggaagac agggcgacct 60 ggaagtccaa ctacttcctt aagatcatcc aactattgga tgattatccg aaatgtttca 120 tcgtgggagc agacaatgtg ggctccaagc agatgcagca gatccgcatg tcccttcgag 180 ggaaggccgt ggtgctgatg ggcaagaaca ccatgatgcg caaggccatc cgagggcacc 240 tggaaaacaa cccagctctg gagaaactgc tgcctcatat ccggggaaat gtgggctttg 300 tgttcaccaa ggaggacctc actgagatca gggacatgtt gctggccaat aaggtgccag 360 ctgctgcccg tgctggtgcc attgctccat gtgaagtcac tgtgccagcc cagaacactg 420 gacttgggcc cgagaagacc tcctttttcc aggctttagg tatcaccact aaaatctcca 480 ggggcaccat tgaaatcctg agtgatgtgc agctgatcaa gactggagac aaagtgggag 540 ccagcgaagc cacgctgctg aacatgctca acatctcccc cttctccttt gggctggtca 600 tccagcaggt gtttgacaat ggcagcatct acaatcctga agtgcttgac atcacagagg 660 aaactctgca ttctcgcttc ctggagggtg tcc 693 // ID JK123722; SV 1; linear; mRNA; EST; MAM; 544 BP. XX AC JK123722; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA91YP24FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-544 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1a478b0479081338ece147acc105f54c. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000009768 (NEDD9) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000016335 CC High quality match with Homo sapiens transcript: ENST00000379446 CC (NEDD9-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000022684 (XM_001089291.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000000873 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1C5L3D04IJCB1, F01W12W01AVVGD, F01W12W01AEWM8, F1BDRJG03F40NP, CC F1C5L3D02DUG3K CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:11206175-11205632. XX FH Key Location/Qualifiers FH FT source 1..544 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 544 BP; 189 A; 79 C; 91 G; 185 T; 0 other; agagaagaag cgcattatct atgatctttt ggtccaattc ttatttagca tttttattcc 60 agcctatttg gaaacatgtt tttaattgca atatatgcct gactgaatta agcttgcttg 120 ttttaaacaa ccaaatcatt ggaacagaaa aggatttaaa aaacaagaat gcatctcaga 180 gtgattaaaa aaaaatcagt ggaaataaat gatcatagaa ggtgcttttc aaaaccactg 240 ctaatacaat tctcaaagtc ctactctgcc aaaagaagac taaaagttat aagttacatg 300 acaaggaaat gtttatgtga gaagagggtt gctgaaaatc aacaacactt gaagtttaaa 360 gtgcgtcttt gtagatttca ttgtataatg tgtatttctt aggagatggc tgacttgatt 420 gatctacgct aagtagagac atttcacgtt tttaaaacca aaatgttcaa tctgtattac 480 tctttgccat cttgtatgta gaggctattt ttaaatcatt aaatttttag atctctgtta 540 tcat 544 // ID JK123723; SV 1; linear; mRNA; EST; MAM; 494 BP. XX AC JK123723; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA01FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-494 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 63a1fe4787577deebebd2eaa025f95cb. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig600 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000026556 (LOC100400548) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000009653 CC High quality match with Homo sapiens transcript: ENST00000484178 CC (SSBP1-010) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000031563 (XM_001084414.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000023826 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000016665 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000036640 (XM_001156207.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000021068 CC (SSBP_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BWDJC02IV0MP, FZ8FYPF01BHYOB, F0DRJ6O01C1CCS, F0BOF8H02IANC3, CC F0M2C4T02HUP0R CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874246|FR874246.1:179920767-179920837. XX FH Key Location/Qualifiers FH FT source 1..494 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 494 BP; 166 A; 80 C; 125 G; 123 T; 0 other; taaggaaaga aaatagaagc catgtttcga agacccgtgt tacaggtact tcgtcagttt 60 gtaagacatg agtccgaaac aactaccagt ttggttcttg aaagatccct gaatcgtgtg 120 catttacttg ggcgagtggg tcaggaccct gtcttgagac aggtggaagg aaaaaatcca 180 gtcacaatat tttctctagc aactaatgag atgtggcgat caggggacag tgaagtttac 240 caaatgggtg atgtcagtca aaagacaaca tggcacagaa tatcagtatt ccggccaggc 300 ctcagagatg tggcatatca gtatgtgaaa aaggggtctc gaatttattt ggaagggaaa 360 atagactatg gtgaatacat ggataaaaat aatgtgaggc gacaagcaac aactatcata 420 gctgataata ttatatttct gagtgaccag acgaaagaga aggagtagaa aggatgattc 480 ttctttggcc atca 494 // ID JK123724; SV 1; linear; mRNA; EST; MAM; 670 BP. XX AC JK123724; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA02FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-670 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 434f66b57166f69de2a32f5d1ca8d9cb. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000009080 (A6ML89_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000003439 CC High quality match with Homo sapiens transcript: ENST00000314606 CC (FARSA-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000006237 (XM_001110025.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000016270 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000016513 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000019456 (XM_001170080.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000011201 CC (SYFA_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0DRJ6O02FYM4F, F0Z1IVZ01CGHDL, F0X19WM02FHXE0, F0Q2XTE02F6YWH, CC F0M2C4T02H2PMT CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/). XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 670 BP; 143 A; 170 C; 251 G; 106 T; 0 other; tacaagtata actggaagca gccatggcgg atggtccggt ggcggaactg ctgctccggc 60 ggctagaggc gtccgatggc ggcctggaca gcgccgagct ggcggctgag ctgggcatgg 120 agcaccaagc ggtggtgggc gccgtgaaga gccttcaggc actgggcgag gtcatcgagg 180 ctgaacttcg gtccaccaag cgctgggagc ttactgcgga gggtgaggag attgcccggg 240 agggcagcca tgaggcccgt gtgtttcgaa gcatcccccc ggagggcctg gcccagagcg 300 agcttatgcg actgcccagt ggcaaagtgg gcttcagcaa ggccatgtcc aacaagtgga 360 tccgggtgga caagagtgca gccgacgggc cccgggtgtt ccgagtggtg gacagcatgg 420 aggacgaggt gcagcggcgg cttcaactgg tccagggggg acaggctgag aagctggggg 480 agaaggagag gagcgagctg aggaagagga agctgttggc tgaagtgacc ctgaagacct 540 actgggtgag caaaggcagt gcctttagta ccagcatctc caagcaagag acagaactga 600 gcccagagat gatctctagt ggctcttggc gggaccggcc cttcaagccc tacaacttct 660 tggcccacgg 670 // ID JK123725; SV 1; linear; mRNA; EST; MAM; 586 BP. XX AC JK123725; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA03FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-586 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; bd4e5357ec644f34a020a78439c1b8d0. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1339 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000043893 (LOC100415114) CC High quality match with Homo sapiens transcript: ENST00000547649 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000000274 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000009348 (XM_001153433.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1GITI002G2KWW, FZ8FYPF02IFCSU, F1C5L3D02C6RT8, FZ8FYPF01EF87H, CC F1HESOY02FOGOJ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874264|FR874264.1:120923686-120923099. XX FH Key Location/Qualifiers FH FT source 1..586 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 586 BP; 137 A; 140 C; 173 G; 136 T; 0 other; gtgtgacttc accgaagacc agaccgcaga gttcaaggag gccttccagc tgtttgaccg 60 aacaggtgat ggcaagatcc tgtacagcca gtgtggggac gtgatgaggg ccctgggcca 120 gaaccccacc aacgccgagg tgctcaaggt cctggggaac cccaagagtg atgagatgaa 180 tgtgaaggtg ctggactttg agcactttct gcccatgctg cagacagtgg ccaagaacaa 240 ggaccagggc acctatgagg attatgttga aggacttcgg gtgtttgaca aggaaggaaa 300 tggcaccgtc atgggtgctg aaatccggca tgttcttgtc acactgggtg agaagatgac 360 agaggaagaa gtagagatgc tggtggcagg gcacgaggac agcaatggtt gtatcaacta 420 tgaagagctc gtccgcatgg tgctgaatgg ctgaggaccc tcccagtctc ccaactccgt 480 gcctttccct gtgtgaattt tgtatctagc ctgaagtttc cctaggctct cttgtctcag 540 cacctttccc atcttgtctc ttggatgatg tttgccgtca gcattc 586 // ID JK123726; SV 1; linear; mRNA; EST; MAM; 473 BP. XX AC JK123726; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA04FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-473 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; b0c24d3bc7dd4ef38ae4acae7eb5cb18. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX02H74Q4, F1BDRJG03GDWA4, FZ8FYPF02JA8UI, F0BOF8H02JRAV5, CC F0X19WM02FYEQ6 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874246|FR874246.1:187634526-187634062. XX FH Key Location/Qualifiers FH FT source 1..473 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 473 BP; 84 A; 126 C; 135 G; 128 T; 0 other; ggcagtgact cctcctccac aggcctcaga tgtgtacctt tctgttggtg ctgacttgga 60 gcagggagga cccctcctgc tctcagagcc tgtgtgtaat cgactcacat ttgagaaaga 120 ctctttctaa ttcagccttc tcctacagac gtgcgtggcc cttatggcga catgcgtcga 180 ggtcagtgga gaacatgggc agatggtgct gcttgcagca gacattgtct tcacgcctca 240 gaactttgct tccctcagcc tgtggggcta ctgctgctct ccttatttga tcctgcccag 300 gaagggtccg gactgtttct tcctgggctg atttcctctt cccgatggtt tctgtgtgac 360 cctggagccg agggcagtga ggcctgttag agaggacagg agcaggtgca gatgtgaggt 420 catgtttcgt cagcacaagt gcactgctgt gtaacctgcg tgctctgcag cag 473 // ID JK123727; SV 1; linear; mRNA; EST; MAM; 686 BP. XX AC JK123727; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA05FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-686 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; b6e5b90f7d746b400db2d5fb37cfa4d6. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3634 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000027034 (LOC100394370) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000011816 CC High quality match with Homo sapiens transcript: ENST00000225724 CC (GOSR1-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000033360 (Q6UIP9_MACMU) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000004752 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000016528 (Q6UIQ0_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000009515 CC (GOSR1_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RTSFR02JF24Q, F1HESOY02HLNN3, F1HESOY02H835I, F0M2C4T02IMDBP, CC F0DTF6N01BTYHO CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874259|FR874259.1:25818201-25818249. XX FH Key Location/Qualifiers FH FT source 1..686 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 686 BP; 250 A; 132 C; 155 G; 149 T; 0 other; aacaaagatg gcggcaggaa ccagcaatta ctgggaagat ctcaggaaac aggctcgaca 60 gctggaaaat gaacttgacc tgaaactagt ttccttcagc aaactatgta cgagttacag 120 tcatagcagt acccgagatg gaagacgcga caggtatagt tctgatacaa cacccctttt 180 aaatggatca agccaagaca gaatgtttga gacaatggcg attgagattg aacaactttt 240 ggcaaggctt acaggggtaa acgataaaat ggcagaatat accaacagtg caggtgtccc 300 ctccttgaat gcggccctga tgcatacatt acagcggcat agagacatat tgcaggatta 360 tacacatgaa ttccataaaa ccaaagcaaa ctttatggca atacgggaaa gggagaatct 420 catgggatca gtgcgaaaag atattgaatc atataaaagt gggtctggag taaacaatag 480 aagaactgag ctatttttga aagaacatga ccaccttcga aactcagatc gtctgataga 540 agagacaata agcattgcta tggcaacaaa agaaaatatg acttcacaga gaggaatgtt 600 gaagtcaatt cacagcaaaa tgaacacttt ggccaatcgt tttcctgctg taaacagcct 660 gatccagagg atcaacctga ggaaac 686 // ID JK123728; SV 1; linear; mRNA; EST; MAM; 636 BP. XX AC JK123728; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA07FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-636 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 06cf19c30598a4bd43723a1e2c90fbf0. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3364 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000058051 CC High quality match with Homo sapiens transcript: ENST00000475533 CC (ZFAND2B-016) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000027542 (XM_001097402.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000011650 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000015546 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000023980 (XM_001146767.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000015343 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000011643 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0OTZ2I02JYN4Q, F0OTZ2I02IT1ON, F1HESOY02IT3IY, F0M2C4T01BSQ2T, CC F0X5I7101DMPXL CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874255|FR874255.1:82776487-82776586. XX FH Key Location/Qualifiers FH FT source 1..636 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 636 BP; 153 A; 184 C; 168 G; 131 T; 0 other; ggcttgagtg agagagaaga acatggaggt ccccctgtac ctgcaatccg actaactctc 60 tgcagatttt ctgccgctta agtgtgatgc ctgcttaggc atcttctgcg cagaccatgt 120 ggcctacgcc cagcatcact gtggatctgc ttaccaaaag gatatccagg tacctgtgtg 180 ccctctctgt aatgtgcctg tgcctgtggc cagaggggag ccccctgacc gtgctgtggg 240 agagcacatt gacagagact gtcgctctga tccagcacag caaaaacgta agatcttcac 300 caataagtgt gagcgcgctg gctgccggca gcgagaaatg atgaaactga cctgtgaacg 360 gtgtagccga aacttctgca tcaagcaccg gcacccactg gaccatgatt gctctgggga 420 ggggcaccca accagccggg caggactagc tgccatctcc agagcacaaa atgtggcttc 480 tacaagcact gtccccagcc caagtcgaac catgccttcc tgtccctctc ccagcagagc 540 cacaacccag tctccatcct ggacagcccc tccagtgatt gctttgcaga atggcctggt 600 gagttgggaa gaggttggat ggacagaaaa caaaca 636 // ID JK123729; SV 1; linear; mRNA; EST; MAM; 699 BP. XX AC JK123729; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA08FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-699 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 9afebf6a459c31c7113db49c724fc35e. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1269 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000060378 (LOC100395223) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000024934 CC High quality match with Homo sapiens transcript: ENST00000461347 CC (NCL-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000025423 (XM_001116949.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000002539 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000064676 CC High quality match with Pongo abelii transcript: ENSPPYT00000015427 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000004354 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1BDRJG03GR60I, F0DTF6N02JN2JT, F0BOF8H01CYZ9V, FZOOKC302IJFS7, CC F1RTSFR01ETSS2 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874258|FR874258.1:1425368-1425573. XX FH Key Location/Qualifiers FH FT source 1..699 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 699 BP; 233 A; 119 C; 188 G; 159 T; 0 other; aaaagtaaag ggattgctta tattgaattt aagacagaag ctgatgcaga gaaaaccttt 60 gaggaaaagc agggaacaga gattgatggg cgatctattt ccctgtacta taccggagag 120 aaaggtcaaa atcaagacta tagaggtgga aagaatagca cttggagtgg tgaatcaaaa 180 actctggttt taagcaacct ctcctacagt gcaacagaag aaactcttca ggaagtattt 240 gagaaagcaa cttttatcaa agtaccccag aaccaaaatg gcaaatctaa agggtatgca 300 tttatagagt ttgcttcatt cgaagatgct aaagaagctt taaattcctg taataaaagg 360 gaaattgagg gcagagcaat caggctggag ttgcaaggac ccaggggatc acctaatgcc 420 agaagccaac catccaaaac tctgtttgtc aaaggcctgt ctgaggatac cactgaagag 480 acattaaagg agtcatttga cggctctgtt cgggcaagga tagtcactga ccgggaaact 540 gggtcctcca aagggtttgg ttttgtagac ttcaacagtg aggaggatgc caaagctgcc 600 aaggaggcca tggaagatgg tgaaattgat ggaaataaag ttaccttgga ctgggccaaa 660 cctaagggtg aaggtggctt tgggggtcgt ggtggaggc 699 // ID JK123730; SV 1; linear; mRNA; EST; MAM; 497 BP. XX AC JK123730; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA09FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-497 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; eb2da65770bf3ef4d351185524ad5761. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3635 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000018055 (LOC100405147) CC High quality match with Homo sapiens transcript: ENST00000361157 CC (IFI6-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000016036 (NP_001041635.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000000808 (IFI6_PANTR) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RHTUQ02G5VRJ, F0M2C4T01AV3FI, F1RHTUQ02GZEOV, F0Z8TLB01AU4N6, CC F0SMK5U02J1HOK CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:30436718-30436646. XX FH Key Location/Qualifiers FH FT source 1..497 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 497 BP; 82 A; 148 C; 172 G; 95 T; 0 other; cgtttactcg ccgctgtgcc catctatcag caggctccgg gctgaagatt gcttctcttc 60 tgtcctccaa ggtctagcga cggagcctgc gcgcggcgcc accatgcggc agaaggcggt 120 atcgcttttc ttgtgctacc tgctgctctt cacttgcagc ggggtggagg caggcaagaa 180 agagtgctcg gagagctcgg acagcggctc cgggttctgg aaggccctga ccttcatggc 240 catcggagga ggactcgcgg tcgccgggct gcctgcgctg ggcttcaccg gcgccggcat 300 cgcggccaac tcggtggctg cctcgctgat gagctggtct gcgatcatga atgggggtgg 360 cgtccccgcc gggggcctag tggccacgct gcagagcgcc ggggctggcg gcagcagcat 420 cgccatgggt aaaataggtg ccctgctggg ctacgccacc cacaagtatc tcgatagtga 480 ggaggatgaa aagaagc 497 // ID JK123731; SV 1; linear; mRNA; EST; MAM; 493 BP. XX AC JK123731; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA10FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-493 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; e66c9f99c7d4d7e5612fde29bbbb6335. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000030307 (GPHN) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000010263 CC High quality match with Homo sapiens transcript: ENST00000478722 CC (GPHN-006) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000039796 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000011828 (XM_001135711.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Q2XTE02HNF72, F0BOF8H02GEDC4 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:130374612-130375034. XX FH Key Location/Qualifiers FH FT source 1..493 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 493 BP; 87 A; 192 C; 119 G; 95 T; 0 other; ctagctgcct tgggtctcgc gctccgcaga gcggcccgag cctctccagc ctcgttccgc 60 cgcctccgcg cgctctcccc gtgcagccac cgacccctcc agctcgcctc cttcctgccg 120 gacttggggt cgcgcgccct gactccgccc cctcccgtgg acccgcgcac tcccggcgcg 180 gcctctcccc cacgcaggcc accgagcact gtacggcctc ccactccttc cccgctctcc 240 tcgcgcttcc ctggctccct agctctcgcg ctttccgcgg cgagcgcgct cccggcccgc 300 gcgctccggg ctccaatttc tcccggctcc tgtcagtgcg gtgactgcgc tgggaaacat 360 ggcgaccgag ggaatgatcc ttactaacca cgaccatcaa atccgtgtcg gagtccttac 420 aggattgaag aataatgaag catattttgg aatttgaaat gtattaaaaa aaaaaaaaaa 480 cctgaaaaag aaa 493 // ID JK123732; SV 1; linear; mRNA; EST; MAM; 590 BP. XX AC JK123732; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA11FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-590 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2f2e9e719e4ba3c91295f0573cfce810. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3636 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000010397 (LOC100412326) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000005118 CC High quality match with Homo sapiens transcript: ENST00000368010 CC (PFDN2-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000002844 (XM_001117749.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000017848 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000002866 (XM_513934.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z1IVZ01BCWFZ, F0DTF6N01BNSII, F0DTF6N01DF3AZ, F0OTZ2I01EGZ3E, CC F1RHTUQ01C6GC8 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:140487553-140487516. XX FH Key Location/Qualifiers FH FT source 1..590 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 590 BP; 165 A; 117 C; 173 G; 135 T; 0 other; agatggcgga gaacagcggt cgcgccggca agagcagcgg gagcggcgcg ggcaaggggg 60 cggtgtccgc agagcaggta attgctggct tcaaccgcct tcggcaggaa cagcgaggcc 120 tggcatccaa agcagctgag ttggagatgg agttgaatga gcacagccta gtgatcgata 180 cactgaagga ggtagatgaa actcgtaagt gctaccgcat ggttggagga gtgctggtgg 240 aacgaactgt caaagaggtg ctgcctgctt tggagaacaa caaggagcag atacagaaga 300 tcattgagac attgacacag cagcttcagg caaaaggaaa agaactaaat gaattccggg 360 aaaagcacaa cattcgtctc atgggagaag atgagaagcc agcagccaag gaaaactcag 420 aaggggctgg ggctaaggcc agctcagctg gagtgttggt ctcctaggga ccaaggcctt 480 tgcatttttt tctaccctga ctcccacttc tgatttctct ttattgttat tattattatt 540 ttctctgcta ttgtaatatt ttttgttaat taaatgtttt ggtcagaaaa 590 // ID JK123733; SV 1; linear; mRNA; EST; MAM; 731 BP. XX AC JK123733; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA12FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-731 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 13872436c69c44ae3c4acffcc2ec240c. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig805 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000011637 (A5HJL4_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000005281 CC High quality match with Homo sapiens transcript: ENST00000369478 CC (CD2-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000017192 (Q6SZ60_MACMU) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000006577 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000058853 (Q6SZ63_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000001179 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H01BAZJV, F0BOF8H01BL7H7, F0BOF8H02JT1KN, F1IUBWX01CAMDZ, CC F1GITI002J1LFY CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:120461178-120461271. XX FH Key Location/Qualifiers FH FT source 1..731 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 731 BP; 259 A; 139 C; 158 G; 175 T; 0 other; cagaatcaaa agaggaaact aactcctaag atgagccttc catgtaaaat tgtaaccagc 60 ttccttctaa ttttcaacgt ttcttccaaa ggtgcagtct ccaaagagat taggaatgct 120 ttggaaacct ggggagccct gggtcaggac atcgacttgg acattcctag ttttcaaatg 180 agtgatgata ttgatgatat aagatgggaa aaaacttcag acaagaaaaa gattgcacaa 240 ttcagaaaag agaagacttt cgaggaaaaa gatacatata agctatttaa aaacggaact 300 ctgaaaatta agcatctgaa gatccatgat caggatagct acaaggtatc aatatatgat 360 acaaacggaa aaaatgtgtt ggaaaaaaca tttgatttga agattcaaga gagggtctca 420 aaaccaaaga tctcctggac ttgcatcaac acaaccctga tctgtgaagt aatgaatgga 480 actgaccccg agttaaacct gtatcaagat gggaaacatc taaaactttc tcagagggtc 540 atcacacaca agtggaccac caacctgagt gcgaaattca agtgcacagc agggaacaaa 600 gtcagcaagg aatccagggt ggagactgtc agctgtacag agaaaggtct ggacatctat 660 ctcatcatcg gcatttgtgg aggaggcagc ctcttgatgg tctttgtgac actgctcgtt 720 ttctacatca c 731 // ID JK123734; SV 1; linear; mRNA; EST; MAM; 305 BP. XX AC JK123734; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA13FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-305 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 7ba1ffa891524e607a6481d4c51095b0. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3559 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000054603 (LOC100404187) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000016087 CC High quality match with Homo sapiens transcript: ENST00000446105 CC (ING4-003) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000023867 (XM_001118270.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000006624 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000005726 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000010047 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000008479 (XM_001169091.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000004970 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF01EJ3KM, F0BOF8H01A7X46, F1IUBWX02I180W, F1RHTUQ01EVMJ3, CC F0X5I7101BAUV0 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:6823698-6823627. XX FH Key Location/Qualifiers FH FT source 1..305 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 305 BP; 93 A; 70 C; 87 G; 55 T; 0 other; ggaagcctat ggcaagtgca aggaatttgg tgacgacaag gtgcagcttg ccatgcagac 60 ctatgagatg gtggacaaac acattcggcg gctggacaca gacctggccc gttttgaggc 120 tgatctcaag gagaaacaga ttgagtcaag tgactatgac agctcttcca gcaaaggccg 180 gactcaaaag gagaagaaag ctgctcgtgc tcgttccaaa gggaaaaact cagatgaaga 240 agcccccaag actgcccaga agaagttaaa gctcgtgcgc acaagtcctg agtatgggat 300 gccct 305 // ID JK123735; SV 1; linear; mRNA; EST; MAM; 488 BP. XX AC JK123735; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA14FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-488 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 3e0d6f4933a003b569b0c4138b85a7ae. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig874 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000024586 CC High quality match with Homo sapiens transcript: ENST00000387347 CC (J01415.4-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000038286 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000054830 CC High quality match with Pongo abelii transcript: ENSPPYT00000024422 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RHTUQ01EBOEB, F0OTZ2I01A6ZT0, FZ8A1BE02IW4CW, F1HESOY01D0U3A, CC F0X5I7102GU3KM CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874248|FR874248.1:86241170-86241484. XX FH Key Location/Qualifiers FH FT source 1..488 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 488 BP; 157 A; 119 C; 95 G; 117 T; 0 other; cgcatgaatg gcatcacgag ggtttaactg tctcttactt tcaaccagtg aaattgacct 60 gtccgtgaag agacggacat gaattaataa gacgagaaga ccctgcggag cttcaattta 120 ctagtacaac taaaaaccac acaaacccac aggtctttaa acccccatgc ctgtactaaa 180 aattttggtt ggggtgacct cggagcacag tcaaacctcc gaacaatcca tgctaagact 240 acacaagcca aagcaaacca atacttgcaa ttgacccaat aacttgatca acggaacaag 300 ttaccccagg gataacagcg caattctatt ctagagtcca tatcaacaat agagtttacg 360 acctcgatgt tggatcagga catcctaatg gtgcagcagc tatcaagggt tcgtttgttc 420 aacgattaaa gtcctacgtg atctgagttc agaccggagc aatccaggtc ggtttctatc 480 tattcaac 488 // ID JK123736; SV 1; linear; mRNA; EST; MAM; 493 BP. XX AC JK123736; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA15FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-493 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; ce6209da8838640b058ad0a61038f870. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2321 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000044238 (LOC100409043) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000001849 CC High quality match with Homo sapiens transcript: ENST00000009589 CC (RPS20-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000029999 (XM_001101671.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000024111 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000037545 (XR_020156.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF02HDH5I, F0Z8TLB02H6O27, FZ8FYPF01EOZBZ, F0BWDJC02IR8BW, CC F1BDRJG03GLM2M CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874258|FR874258.1:103626920-103627410. XX FH Key Location/Qualifiers FH FT source 1..493 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 493 BP; 139 A; 114 C; 123 G; 117 T; 0 other; gtaggacctg cggatctttg gttcgcacgc tcctgctcct gactcaccgc tgttcgctct 60 cgccgaggaa caagtcggtc aggaagccgc gcagcagcca tggcttttaa ggataccgga 120 aaaacacccg tggagccgga ggtggcaatt caccgaattc gaatcaccct aacgagccgc 180 aacgttaaat ccctggaaaa ggtgtgtgct gacttgatca gaggcgcgaa ggaaaagaac 240 ctgaaagtga aaggaccagt tcgaatgcct accaagactt tgagaatcac tacaagaaaa 300 actccttgtg gtgaaggttc taagacatgg gatcgtttcc agatgagaat ccacaagcga 360 ctcattgact tgcacagtcc ttctgagatt gttaagcaga ttacttccat cagtattgag 420 cctggagttg aggtggaagt caccattgca gatgcttaag tcaactattt taataaattg 480 attaccagtt gtt 493 // ID JK123737; SV 1; linear; mRNA; EST; MAM; 221 BP. XX AC JK123737; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA16FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-221 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1c4d229c3332bbf7dc0f4df4eaa7af8b. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0OTZ2I01D4D7I, F0BOF8H02JLFUE, F0OTZ2I01D4D7I, F1RTSFR02JVCR4, CC F0BOF8H02JLFUE CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:8452221-8452262. XX FH Key Location/Qualifiers FH FT source 1..221 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 221 BP; 73 A; 49 C; 38 G; 61 T; 0 other; gtgagcattt attcatgtgc caaagcctgt actgaagacc cctactgtgc acacatggag 60 agaacatgag tagaatagtt gacagaccac ctaaattata tagacctcct ttacacataa 120 ataagaagag cttgttatct atccctacaa tgacagatgc caatccaacc acttgttaat 180 ggagaatgtt caattcataa gtggatcttc gtcatctcac t 221 // ID JK123738; SV 1; linear; mRNA; EST; MAM; 635 BP. XX AC JK123738; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA17FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-635 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 9e5589aa8ea1089481e66b807709464c. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2275 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000013265 (LOC100409214) CC High quality match with Homo sapiens transcript: ENST00000368985 CC (CTSS-001) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000012728 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000002341 (XM_001171268.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000001074 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z1IVZ02F7NAJ, F1HESOY01EOAAT, F0X5I7102JOD83, F1C5L3D02DYLD4, CC F1BDRJG03GKBJO CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:129951598-129951472. XX FH Key Location/Qualifiers FH FT source 1..635 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 635 BP; 184 A; 126 C; 168 G; 157 T; 0 other; catcaattca aggattctta ctgtgggagc acctgctggt tctatcacaa tgaaacggct 60 ggtttgtgtg ctcttggtgt gctctgcagt ggcgcagttg cataaagatc ctaccctgga 120 tcatcactgg catctctgga agaaaaccta tggcaaacaa tacaaggaaa agagtgaaga 180 agcagtacga cgtctcatct gggaaaagaa tctaaagttt gtgatgcttc ataacctgga 240 gcattcaatg ggaatgcact catatgatct gggcatgaac cacctgggag acatgaccag 300 tgaagaagtg atgtctttga tgagttccct gagagttccc agccagtggc agagaaatat 360 cacatataag tcaaacccta atcagatatt gccggattct gtggactgga gagagaaagg 420 gtgtgttact gaagtgaaat atcaaggttc ttgtggtgct tgctgggctt tcagtgctgt 480 gggggccctg gaagcacagc tgaagctgaa aacaggaaag ctggtgtctc tcagtgccca 540 gaacctggtg gattgctcaa ctgaaaaata tggaaacaaa ggctgcaatg gtggcttcat 600 gacaacggct ttccagtaca tcattgataa caacg 635 // ID JK123739; SV 1; linear; mRNA; EST; MAM; 631 BP. XX AC JK123739; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA18FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-631 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 031ff260e3df11a0297771fe72e9f38b. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig501 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1C5L3D04IA7SO, F1HESOY01BKG31, F0Q2XTE02I6KA9, FZOOKC302FL4ZZ, CC F01W12W01BJ8BS CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/). XX FH Key Location/Qualifiers FH FT source 1..631 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 631 BP; 148 A; 211 C; 173 G; 99 T; 0 other; ctgagatcag agcacggagc tcagctgcac caggctgaat tccccatggg tcctgtgacc 60 tcagtgcatg gggggaccta caggtgcatc agctcacgca gcttctccca ctacctgctg 120 tcacgcccca gtgaccccgt ggagctcaca gtcttaggat ccttggagag tcccagcccc 180 tcacccacaa ggtccatctc agcagctggc tctgaggacc agcccctcat gcctacaggg 240 tcagaccccc agagtgccca gagacaggct gatttccaac gtcctccagg ggctgcagag 300 ccagagccca aggatggggg tctacagagg aggtccagcc cagctgctga cgtccaggga 360 gaaaacccca atgctgccgt gaaggacaca cagcctgagg acggggtgga gcttgacagt 420 caggctgcta catctgaagc cccccaggat gtgacctacg cccagctgca gagcttgacc 480 ctcagacggg aggcaactga gcctcctcca tcccagaaac gggaaccttc agctgagccc 540 agtgtctatg ccactctggc catccactaa tccagggagg acccagaccc cacaaaccac 600 ggagactcag gaccccagaa ggcatggaag t 631 // ID JK123740; SV 1; linear; mRNA; EST; MAM; 536 BP. XX AC JK123740; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA19FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-536 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 87c7526d2edca617086d090fdb7f6575. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1494 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000042143 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000030286 (Q3I231_9PRIM) CC High quality match with Homo sapiens transcript: ENST00000355857 CC (DBI-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000015551 (XM_001103096.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000013156 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000047421 (XM_515759.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z1IVZ02FKD3W, F0Z1IVZ02JXJLX, F1HESOY02IN09K, F0DTF6N01ASADB, CC F1HESOY02JJWLR CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874253|FR874253.1:85370969-85370470. XX FH Key Location/Qualifiers FH FT source 1..536 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 536 BP; 155 A; 116 C; 137 G; 128 T; 0 other; aggtcctcgc ctcctccgca gtctccttgg agttcttgca aggcggccag gatgtctcag 60 gctgagtttg agaaagctgc agaggaggtt aagcacctta agaccaagcc agccgatgat 120 gagatgctgt tcatctatgg ccgctacaaa caagcaaccg tgggcgacat aaatacagaa 180 cggcccggga tgttggactt cacgggcaag gccaagtggg atgcctggaa tgagctgaaa 240 gggactacca aggaagatgc catgaaagct tacatcgaca aagtagaaga gctaaagaaa 300 aaatacggga tatgagtgac tggatttggt tactaggcca tgtgtttctc ccaaactgag 360 acaatgcctt gttttttcta ataccgtgga tggtgggaat tccggaaaat aaccagttag 420 cccagctact caaggctgct caccatacgg ctctaacaga ttagaggcta aaacgattac 480 tgacctccct cgagtagttt ttatctgaga tcaattaaaa gtgtatttgt tacttt 536 // ID JK123741; SV 1; linear; mRNA; EST; MAM; 217 BP. XX AC JK123741; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA20FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-217 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 9399f2b951b099a57962663161891162. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3026 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX01CKLEI CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:23283356-23283191. XX FH Key Location/Qualifiers FH FT source 1..217 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 217 BP; 74 A; 34 C; 23 G; 65 T; 21 other; ggccgcacaa ctttgtacaa gaaagttggg nnnnnnnnnn nnnnnnnnnn ncaagtttca 60 tgtttttatt taactcgggg aacaaaaatg aaccaaatta actgcaactt tatcaaatgt 120 ataagaagta aatatgaatc ttatatgaca aaatgtttta ctcattataa caaatttcca 180 ataaccccat caattatatt tctaaatttt acctcct 217 // ID JK123742; SV 1; linear; mRNA; EST; MAM; 639 BP. XX AC JK123742; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA21FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-639 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 72d87fccf14d79e13590781a76f1159b. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2372 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000023733 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000001527 CC High quality match with Homo sapiens transcript: LRG_126_t1 CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000030321 (XM_001101570.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000002049 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000022764 (XM_515637.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RHTUQ01CITX4, F1HESOY01B3LE0, F0BOF8H02JNXDX, F01W12W01D2WEF, CC F0BOF8H02I5GGQ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874256|FR874256.1:98050364-98050712. XX FH Key Location/Qualifiers FH FT source 1..639 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 639 BP; 113 A; 215 C; 204 G; 107 T; 0 other; cctctccaaa gcactgggcg ccgatgacag ttattacact gcccgctcag cagggaagtg 60 gccgctcaag tggtacgcac ccgagtgcat caacttccgc aagttctcca gccgcagcga 120 cgtctggagc tacggggtca ccatgtggga ggccttgtcc tacggccaga agccctacaa 180 gaagatgaag gggccggagg tcatggcctt catcgagcag ggcaagcgga tggagtgccc 240 atcagagtgt ccgcctgaac tgtacgaact catgagcgac tgctggatct acaagtggga 300 ggatcgcccc gacttcctga ccgtggagca gcgcatgcgg gcctgttact acagcctggc 360 cagcaaagcg gaggagcccc cgggctgcgc acagaaggcc gaggctgcct gtgcctgagc 420 tcccaccacc cagggcctgc agccctccac gccggctctt cccctgcctc ggccccaccc 480 caggccctgc agcctggctg agccctgctt ggttgtctcc acacacagcg gggctgtggt 540 aggggcgtct caggccgtgc tggccttgca tcccctgcct ggcccctgtc ctgtctggct 600 gcggagcagg gaggccccgg aaggtggggc agtgcagcc 639 // ID JK123743; SV 1; linear; mRNA; EST; MAM; 625 BP. XX AC JK123743; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA22FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-625 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 0fde01ef92f1468a339278ea0a6ca2ee. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3026 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000063060 (B2MG_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000023544 CC High quality match with Homo sapiens transcript: ENST00000349264 CC (B2M-001) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000007249 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000012956 (B2MG_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000007591 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX01CKLEI, FZ8FYPF02HY8IJ, FZ8FYPF02HIYM6, FZ8FYPF02FN0ES, CC F0BWDJC02H5DLY CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:23282842-23283090. XX FH Key Location/Qualifiers FH FT source 1..625 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 625 BP; 165 A; 130 C; 143 G; 187 T; 0 other; cgagatgtct cgctcagtgg ccttagccgt gctggcgcta ctctttcttt ctggcctgga 60 ggctatccag cgtgctccaa agattcaggt ttactcacgc catccaccag agaatggaaa 120 gccaaatttc ctgaattgct atgtgtctgg gtttcatcca tctgatattg aagttgactt 180 actgaagaat ggagagaaaa tggggaaagt ggagcattca gacttgtctt tcagcaaaga 240 ctggtctttc tatctcttgt actacactga attcaccccc aatgaaaaag atgagtatgc 300 ctgccgtgtg aaccacgtga ctttgtcagg gcccaggaca gttaagtggg atcgagacat 360 gtaagcagca tcatggaggt ttgaagatgc cgcatttgga ttggatgaat tccaaattct 420 gcttccttgc tttttaatat tgatatgctt atacacttta tgcaaaaaat gcagagttat 480 aataatgtta acacaggcat gatcttcttt ataattctac tttgagtgct atctccacgt 540 ttgaggtatc tgggcctcta gctttaggag ggctcgcaac ttagaggtgg ggagcagaga 600 attctcatat ccaacatcaa catct 625 // ID JK123744; SV 1; linear; mRNA; EST; MAM; 361 BP. XX AC JK123744; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YA23FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-361 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 3ad3932430f61447c7624d28679b9dac. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000027700 (EED) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000022871 CC High quality match with Homo sapiens transcript: ENST00000528250 CC (EED-011) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000027560 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000020710 CC High quality match with Pongo abelii transcript: ENSPPYT00000004458 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0SMK5U02HAK4V, FZ8A1BE02H9E6D, F0OTZ2I02HY8LJ, F1RHTUQ02IHZ61, CC F0DRJ6O02F9BFZ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:85261532-85261846. XX FH Key Location/Qualifiers FH FT source 1..361 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 361 BP; 99 A; 60 C; 77 G; 125 T; 0 other; aactttatgt ttgggattta gaagtagaag atcctcataa agccaaatgt acaacactga 60 ctcatcataa atgtggtgct gctattcgac aaaccagttt tagcagggat agcagcattc 120 ttatagctgt ttgtgatgat gccagtattt ggcgctggga tcgacttcga taaaatactt 180 ttgcctaatc aaaattagag tgtgtttgtt gtctgtgtaa aatagaatta atgtatcttg 240 ctagtaaggg cacgtagagc atttagagtt gtctttcagc attcaatcag gctgagctga 300 atgtagtgat gtttacattg tttacattct ttgtactgtc ttcctgctca gactctactg 360 c 361 // ID JK123745; SV 1; linear; mRNA; EST; MAM; 610 BP. XX AC JK123745; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB01FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-610 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; df10544b0a2faa0b2e85e7260655d45f. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2101 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000039703 CC High quality match with Homo sapiens transcript: ENST00000335295 CC (HBB-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000006876 (HBB_MACMU) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000006177 (HBB_PANTR) CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE02JXKN0, FZ8A1BE02IQM7U, F0M2C4T01BCZEQ, F1IUBWX01EIKD3, CC F1HESOY02FXE10 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:68763681-68763944. XX FH Key Location/Qualifiers FH FT source 1..610 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 610 BP; 134 A; 151 C; 166 G; 159 T; 0 other; gacacaactg tgttcactag caacctcaga caccatggtg catctgactc ctgaggagaa 60 gactgccgtt accaccctgt ggggcaaggt gaacgtggat gaagttggtg gtgaggccct 120 gggcaggctg ctggtggtct acccttggac ccagaggttc tttgagtcct ttggggatct 180 gtcctctcct gatgctgtta tgggcaaccc taaggtgaag gctcatggca agaaagtgct 240 tggtgccttt agtgatggcc tggctcacct ggacaacctc aagggcacct ttgcccagct 300 gagtgagctg cactgtgaca agctgcatgt ggatcctgag aacttcaagc tcctgggcaa 360 cgtgctggtg tgtgtgctgg cccatcactt tggcaaagaa ttcaccccgc aagtgcaggc 420 tgcctatcag aaagtggtgg ctggtgtggc taatgccctg gcccacaagt accactaagt 480 tcactttctt gctgtccaat ttctaccaaa ggttcctttg ttcccaaagt ccaactactg 540 aactggggga tattatgaag ggccttgagg atctggattc tgcctaataa aaaacattta 600 ttttcattgc 610 // ID JK123746; SV 1; linear; mRNA; EST; MAM; 658 BP. XX AC JK123746; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB02FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-658 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 8d2386adbf2af5ab58a6c95113891ffb. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3637 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000055988 CC High quality match with Homo sapiens transcript: ENST00000356709 CC (BTN2A2-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000006682 (XM_001089758.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000042859 CC High quality match with Pongo abelii transcript: ENSPPYT00000018983 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02JZ76E, F1RHTUQ02G3L5P, F1C5L3D02ELIUE, F1IUBWX01D6RR6, CC F1HESOY02IOOAT CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:26567270-26567749. XX FH Key Location/Qualifiers FH FT source 1..658 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 658 BP; 203 A; 117 C; 154 G; 184 T; 0 other; ggaaccagat acgcaggtca gagatagagg aagtggaacc agagagctgg gaggcaccaa 60 ggctgtaagg atggctaagt cccaccgtaa cagctaaagg gtcctgggaa atgatggctc 120 atttccaccc aaccccagga tttccacagc acacacccac aagcctggac ctgggacgaa 180 gatgaatgaa gaacatggac tcatgcggat gtggtttggc ccacttgtcc ctgcaataaa 240 caaggagtca gtacttagtc cctgagtgtg gttgaggttt gaggtcctgg ttgagcaggg 300 aagtactgga cctggtctgc atcagacttc aggttcaatg gggacaccag tggcttcaaa 360 cttcctgatc taattatgtc tttggacact tagaaattat tgaggacctt agagagcttt 420 tgttcatttg ggttaatatt tgttaatatt tatggcattt gacattgaaa caaaaattta 480 aaatgttatc ttttaattca tgttaaaata gcatgaataa atcaattata ggttaatata 540 aataggatgt tttgtgaaaa agcaatctat tgcgtccaaa taaaagaaca agaagtgtga 600 cactggttaa ctttttccaa atctaatgtc tggcttaata gaagatgttt gtattctc 658 // ID JK123747; SV 1; linear; mRNA; EST; MAM; 386 BP. XX AC JK123747; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB03FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-386 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 62c1e8bf64048d5b7fd4f4a806bd78f7. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0SMK5U02IAZ7V, FZ8A1BE01EXFHA, FZ8A1BE01AS58W, F0X19WM01BR6YE, CC F1IUBWX02JZP41 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874261|FR874261.1:73694729-73694502. XX FH Key Location/Qualifiers FH FT source 1..386 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 386 BP; 104 A; 80 C; 119 G; 83 T; 0 other; ctgttcaata gtattgacaa atgggcccat ttttaatgga acaatagaaa gtgaaatgga 60 gactggggag gaagtatggt gagcaagact cagcagttct cagaagtgtc ccagcacggt 120 ggctcacacc tgtaaccagc actttgggag gccaagatgg gcagatgatc tgtggccagg 180 agtttgagac cagcccggcc aacatggtga aaccctgtgt ccactaaaaa tacaaaaatt 240 agctgggcat ggtggtgcac gcctatagtc ccagctactt gggaggctaa ggcaggagaa 300 tcacttgaac ctggcaggtg gaggttgcag tgagctgaga tcgtgctgct gtgctctagc 360 ctgggtgaga gagcgagact ctgtcg 386 // ID JK123748; SV 1; linear; mRNA; EST; MAM; 630 BP. XX AC JK123748; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB04FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-630 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; df962c858c280131fb9358b5555ee7af. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1952 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02FRTPA, FZ8FYPF01BIGK8, F0DRJ6O01EGGM7, F0M2C4T02JAP28, CC F1HESOY02GN269 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:10485877-10485248. XX FH Key Location/Qualifiers FH FT source 1..630 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 630 BP; 143 A; 122 C; 113 G; 252 T; 0 other; gagtgacttt tcataagaaa aatattttat ttacattatt tgaacttttg aggtttcata 60 taaatatggt ttgatttttc ctgttcctgt ttggtggaca aggatcctag ttagagagct 120 ccctcttctg tcagttctgt cctattcagc acagcgtctt ctatgggtgg tggtgatgat 180 gggaatagga agggcaaatg atagtcatgt cttttatctc tttcttttct gcaggattct 240 taagttcctc aaattactta tttactttga tagtcatatt tgtaagaagt gtcacccctt 300 ctttattttc cacttctctc ccagaaacca ttgctttcct ttccaagttt cccacttctg 360 gtcttgcgtt tcaagccttt tcccagtact atgaactacc aagtcccaaa ttgtgttaag 420 tactctgaca tttattgttg aagttgtatc tttcagggtt tattatatct gtgattcatt 480 tacgtcctct agtattctct tctgagtatg ttaaagacta atctccctac cctccaccgc 540 aggtgttcag aggtaggcag gagcaagtgt tcttctgcaa ttatttttct ctatttatag 600 gtaatttgaa ggttgtggta ctctatgtct 630 // ID JK123749; SV 1; linear; mRNA; EST; MAM; 664 BP. XX AC JK123749; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB06FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-664 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; ffeb26ae709676faab007b76549e5c19. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3638 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000031024 (LOC100393982) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000013946 CC High quality match with Homo sapiens transcript: ENST00000358514 CC (PSMB10-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000016671 (XM_001095570.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000012284 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000006425 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000015585 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1C5L3D04IV00O, F0X19WM01DDEW8, F0BWDJC01BYCES, F1HESOY01DQLAU, CC F0BWDJC01AI3FI CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874263|FR874263.1:66305511-66305354. XX FH Key Location/Qualifiers FH FT source 1..664 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 664 BP; 130 A; 223 C; 194 G; 117 T; 0 other; cagacgtgga gcctagcaga ggacttttca gttcctcacg ggccccactt gtctgaccga 60 ctcatccacc cacgacccct aatccgctct gcctgcccca agatgctgaa gccagccttg 120 gagccccgag ggggcttctc cttcgagaac tgccaaagaa atgcatcctt ggaacgcgtc 180 ctcccggggc tcaaggtccc tcatgcacgc aagaccggga caaccatcgc gggccttgtg 240 ttccaagacg gagtcattct gggcgccgac acgcgagcca ctaacgattc ggtcgtggcg 300 gacaagagct gtgagaagat ccacttcatc gcccccaaaa tctactgctg tggggctgga 360 gtagccgctg acgccgagat gaccacaagg atggtggcgt ccaagatgga gctacacgcg 420 ctatccacgg gccgagagcc ccgcgtggcc acggtcactc gcgtcctgtg ccagacgctc 480 ttccggtacc ggggccacgt gggtgcatcg ctgatcgtgg gcggcgtaga cctgactgga 540 ccgcagctct acagcgtgca tccccatggc tcctacagcc gtctgccctt cacagccctg 600 ggctctggcc agggcgaggc cctggcggta ctagaagacc ggttccagcc gaacatgacg 660 ctag 664 // ID JK123750; SV 1; linear; mRNA; EST; MAM; 670 BP. XX AC JK123750; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB07FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-670 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 5120032dd6f07a8a6f59e0192c590198. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000021058 (A6MKY9_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000006912 CC High quality match with Homo sapiens transcript: ENST00000248450 CC (AAMP-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000032105 (XR_010777.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000006828 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000010897 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000001791 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000023937 (XM_001154321.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000015307 CC (AAMP_PONAB) CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000001981 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T02J4E1M, F0BWDJC02FQXYG, F0DTF6N01CWGB7, F1HESOY01DYCAD, CC F0Z1IVZ01DBW0F CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874255|FR874255.1:81802593-81802410. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 670 BP; 146 A; 159 C; 235 G; 130 T; 0 other; gcagagctga ctctgggagg cgtttgggcc cagagaagtg gatcggccgc ttgcgccgca 60 tggagtccga atcggaaagc ggggctgctg ctgacacccc cccactggag accctaagct 120 tccatggtga tgaagagatt atcgaggtgg tagaacttga tcccggtccg ccggacccag 180 atgacctcgc ccaggagatg gaagatgtgg actttgagga agaagaggag gaagagggca 240 acgaagaggg ctgggttcta gaaccccagg aaggggtggt cggcagcatg gagggccccg 300 acgatagtga ggtcaccttt gcattgcact cagcatctgt gttttgtgtg agcctggacc 360 ctaagaccaa caccttggca gtgaccgggg gtgaagatga caaagccttt gtatggcggc 420 tcagcgatgg ggagctgctc tttgagtgtg caggccataa agactctgtg acttgtgcgg 480 gtttcagcca tgactccact ctagtggcca caggggacat gagtggcctc ttgaaagtgt 540 ggcaggtgga caccaaggag gaggtctggt cctttgaagc gggagacctg gagtggatgg 600 agtggcatcc tcgggcacct gtcctgttgg cgggcacagc tgacggcaac acccggatgt 660 ggaaggtccc 670 // ID JK123751; SV 1; linear; mRNA; EST; MAM; 685 BP. XX AC JK123751; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB08FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-685 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 049387d8551237918100861dacf1401e. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0DRJ6O01C0HF4, F0Z8TLB01D4LHO, F0Z1IVZ02F12NM, F0DRJ6O01EGIRK, CC F0BOF8H02IR7E7 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874246|FR874246.1:187389826-187390050. XX FH Key Location/Qualifiers FH FT source 1..685 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 685 BP; 215 A; 103 C; 159 G; 136 T; 72 other; accagttatc tttaagaacg gtcagtttag gggctggtgc aaaggatgaa ttgcacattg 60 ttgaagcaga ggcaatgaat tatgaaggca gtccaattaa agtaacactg gcaactttga 120 aaatgtctgt acagccaacg gtttcccttg ggggctttga aataacacca ccagtggtct 180 taaggttgaa gtgtggttca gggccagtgc atattagtgg acagcactta gtagctgtgg 240 aggaagatgc agagtcagaa gatgaagagg aggaggatgt gaaactctta agtatatctg 300 gaaagcggtc tgcccctgga ggtggtagca aggttccaca gaaaaaagta aaacttgctg 360 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 420 nnnnnnnnnn nnnggaagct gaagaaaaag caccagtgaa gaaaggacaa gaatccttca 480 aaaaacagga aaaaactcct aaaacaccaa aaggacctag ttctgtagaa gacattaaag 540 caaaaatgca agcaagtata gaaaaaggtg gttctcttcc caaagtggaa gccaagttca 600 tcaattatgt gaagaattgc ttccggatga ctgaccaaga ggctattcaa gatctctggc 660 agtggaggaa gtctctttaa gaaaa 685 // ID JK123752; SV 1; linear; mRNA; EST; MAM; 668 BP. XX AC JK123752; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB09FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-668 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 0a285472508b62f4535c8f01aba4120a. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3639 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000036407 (LOC100412622) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000002560 CC High quality match with Homo sapiens transcript: ENST00000228434 CC (CD69-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000021448 (XM_001114683.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000003806 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000008619 (XM_520732.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000005047 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000014346 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1HESOY01BFEG4, F0DTF6N02G323O, F0ZWYBK01ALQ1O, F1IUBWX01EYDLU, CC FZ8A1BE01C8WAJ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:9925491-9925368. XX FH Key Location/Qualifiers FH FT source 1..668 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 668 BP; 205 A; 138 C; 145 G; 180 T; 0 other; caaagacttt cactgtagat catcttgacc tcagattaac tagggaatct tgagaataaa 60 gatgagctct gatcattgtt acgtaacaga gaacagctct ttgcatccgg agagtggaca 120 agaaaatgac gccaccagcc cccggttttc aacacatcgt gaaggatcct tccaagttcc 180 tgtcctgtgt gctgtaatga atgtggtctt catcaccatt ttaatcatag ctctcattgc 240 cttatcagtg ggccaataca attgtccagg ccaatacaca ttctcaatgc catcagatag 300 ccatgtttct tcatgctctg atgactgggt tagctaccag aggaaatgct actttatttc 360 tactgtgaag aggagctgga cttcagccca aaatgcttgt tctgaacatg gtgctactct 420 cgctgtcatt gattctgtga aggacatgaa ctttctaaaa cgatacgcag gtggagatga 480 acactgggta ggactgaaaa aggaacctgg tcacccatgg aagtggtcaa atggcaaaga 540 atttaacaac tggttcaact ttacagggtc tgagaagtgc gcttttctga aaaacacaga 600 agtcagcagc atggaatgtg agaagaattt atactggata tgtaacaaac cttacaaata 660 ataaggaa 668 // ID JK123753; SV 1; linear; mRNA; EST; MAM; 670 BP. XX AC JK123753; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB10FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-670 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 44692d2928af597c303c6c2814203076. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2522 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000038114 (LOC100414504) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000014155 CC High quality match with Homo sapiens transcript: ENST00000418105 CC (ICAM2-202) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000020550 (Q5NKU8_MACMU) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000019255 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000017489 (ICAM2_PANTR) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02HVZMI, F0Q2XTE01DK3M7, F0M2C4T01ETALF, F0BWDJC02JCPWM, CC F1RHTUQ02IKNTV CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874259|FR874259.1:59702382-59702274. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 670 BP; 131 A; 198 C; 182 G; 159 T; 0 other; aagcttctca ctttccctcc agtgaagctt ggcggcatgg gaagaatttt ggcggtgtcg 60 aggtctgagt tttagtgcgt tcccctctct gggcatcagt ttctccctgg cagcccttcg 120 ctggtccctg caagcccgtg gacactgccg gagatgtcct cttttggttt cgggactctg 180 accatggccc tctttgccct ggtctgctgt tcaggatctg atgagaaggc attcgaggta 240 catatgaggc tagagaagct gatagtaaag cccaaggagt ccttcgaggt caactgcagc 300 accacctgta accagcctga agtgggtggt ctggagactt ctttaaataa gattctgctg 360 ctcgaacagg ctcagtggaa gcattacttg atctcaaaca tctcccatga cacggtcctc 420 tggtgccact tcacctgctt tgggaagcag aagtcaatga gttccaacgt cagcgtgtac 480 cagcctccaa ggcaggtctt cctcacactg cagcccactt gggtggccgt gggcaagtcc 540 ttcaccatcg agtgcagggt gcccgccgtg gagcccctgg acagcctcac cctcagcctg 600 ctccgtggca gtgagactct gcacagtcag accttccgga aggcagcccc tgccctgccg 660 gaggccacag 670 // ID JK123754; SV 1; linear; mRNA; EST; MAM; 638 BP. XX AC JK123754; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB11FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-638 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 6483a1365621928d1ee00203c29d13c8. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000023198 CC High quality match with Homo sapiens transcript: ENST00000533256 CC (EPS8L2-003) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000010903 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X19WM01EVPTF, F0DTF6N01ECPFB, FZ8FYPF02GCGZK, FZ8FYPF02GGELL, CC F1HESOY02HFR7K CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:533670-533808. XX FH Key Location/Qualifiers FH FT source 1..638 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 638 BP; 140 A; 184 C; 229 G; 85 T; 0 other; ctgggcagaa gggaagggct ggtccacagg ccaggggagg ggtggggtcc tgggcaggtg 60 ctgggaggcg ggggcagggc atggggagcc tgatcacctc ccgcccccgc aggtcggtca 120 gaagtactgg ggccccgcca gcccgaccca caagttgccc ccaagcttcc cggggaacaa 180 agatgagctc atgcagcaca tggacgaggt caacgacgag ctcatccgga aaatcagcaa 240 catcagggcg cagccacagc ggcacttccg cgtggagcgc agccagcccg tgagccagcc 300 gctcacctac gagtcgggtc ccgacgaggt ccgcgcctgg ctggaagcca aagccttcag 360 cgctcggatc gtggagaacc tgggaatcct gactgggccg cagctcttct ctctcaacaa 420 ggaggagctg aagaaggtgt gcggggagga gggcgttcgc gtgtacagcc agctcaccgt 480 gcagaaggcc ttcctggaga agcagcaaag tgggtcggag ctggaagaac tcatgaacaa 540 gtttcattcc atgaatcaga gaagggggga ggacagctag gcccggctgc cgccagcggg 600 ggcctgcgga ggggaagccc acgcccaatg catggagt 638 // ID JK123755; SV 1; linear; mRNA; EST; MAM; 702 BP. XX AC JK123755; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB13FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-702 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2d3824f32a607cc7f058f1169c56ad99. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1180 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000019570 (B0KWA9_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000023460 CC High quality match with Homo sapiens transcript: ENST00000309268 CC (EEF1A1-009) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000015401 (Q3YAI9_MACMU) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000015309 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000005760 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000011455 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000048233 (EF1A1_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000019520 CC (EF1A1_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0OTZ2I02JZ7FW, F1RHTUQ02HVYYQ, F1HESOY01A5BGV, F0BOF8H01EMS2I, CC F1RHTUQ02IXMRF CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874251|FR874251.1:55790193-55789564. XX FH Key Location/Qualifiers FH FT source 1..702 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 702 BP; 211 A; 145 C; 179 G; 167 T; 0 other; acgggtttgc cgccagaaca caggtgtcgt gaaaactacc cctaaaagcc aaaatgggaa 60 aggagaagac tcatatcaac attgtcgtca ttggacacgt agattcgggc aagtccacca 120 ctactggcca tctgatctac aaatgcggtg gcatcgacaa aagaaccatt gaaaaatttg 180 agaaggaggc tgctgagatg ggaaagggct ccttcaagta tgcctgggtc ttggataaac 240 tgaaagctga gcgggaacgt ggtatcacca ttgatatctc cttgtggaaa tttgagacca 300 gcaagtatta tgtgactatc attgatgccc caggacacag agacttcatc aaaaacatga 360 ttacagggac atctcaggct gactgtgctg tcctgattgt tgctgctggt gttggtgaat 420 ttgaagctgg tatctccaag aatgggcaga cccgagagca tgctcttctg gcttacacac 480 tgggtgtgaa acaactaatt gttggtgtta acaaaatgga ttccactgag ccaccctaca 540 gccagaagag atacgaggaa attgttaagg aagtcagcac ttacattaag aaaattggct 600 acaaccccga cacagtagca tttgtgccaa tttctggttg gaatggtgac aacatgctgg 660 agccaagtgc taacatgcct tggttcaagg gatggaaggt ca 702 // ID JK123756; SV 1; linear; mRNA; EST; MAM; 505 BP. XX AC JK123756; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB14FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-505 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 4745760219f90da8151490ede507351d. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3640 CC High quality match with Homo sapiens transcript: ENST00000346437 CC (CD6-202) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000017552 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000049790 (XM_001143860.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX01C4FRE, F0BOF8H01DTVXV, F1IUBWX01EM1LP, F1GITI001EDVFB, CC F0X19WM02JDXMG CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:13262187-13261985. XX FH Key Location/Qualifiers FH FT source 1..505 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 505 BP; 98 A; 168 C; 174 G; 65 T; 0 other; ggaggggaga gagcagcgag acacaggaac aggaacagcg aagggcagag cagacctgcg 60 ccaggggcgc acaacggccg tgtccacctc ccggccccaa aatggtgctt cccacaggca 120 gccacgtgta gcagccagag acagctccag acatgtggct tttctttggg atcgctggat 180 tgctgacagc agccctctca ggtcatctgt ctccagcccc acctgaccag ctcaacgcca 240 gcagtgcaga gagcgaggtc tgggagccag ggcagcggct tccggtccgt ctgacgaacg 300 ggagcagcag ctgcagcggg acggtggagg tccggctcaa ggcgtcctgg gagcctgcgt 360 gctgggcgct ctgggacagc cgcgccgcag aggccgtgtg ccgcgcactg gactgcggcg 420 gggcggaggc atccaccctg ctcccccggc cgaccccgga gctgcctccc ccgccggcag 480 ctgggaacac aagcgctgca actaa 505 // ID JK123757; SV 1; linear; mRNA; EST; MAM; 710 BP. XX AC JK123757; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB15FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-710 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1ec4d178007f81f95a3c10f119ba83f8. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2999 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000053416 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000012435 CC High quality match with Homo sapiens transcript: ENST00000392254 CC (PYHIN1-003) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000006360 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000016756 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000042973 CC High quality match with Pongo abelii transcript: ENSPPYT00000000806 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF01DR6KP, F1RTSFR01B6LIL, FZ8FYPF02I021R, F1GITI002GDBJ9, CC F0X19WM01CNBVI CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:138343397-138343684. XX FH Key Location/Qualifiers FH FT source 1..710 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 710 BP; 258 A; 146 C; 130 G; 176 T; 0 other; gaaaacactt cattttctta gcatttcaga agattataac atcctgtatt tcagtttctg 60 agagctttac tgattgattt ccctactcaa aacaatcctc atttcctaca tttctgaaga 120 tctcaagatc tggactactg ttgaagaaat tcccagtaag gctcacttat atctttagcg 180 atggcaagta actacaagaa aattgttcta ctaaaaggat tggaggtcat caatgattat 240 cattttagaa ttgttaagtc cttactgagc aacgatttaa aacttaatcc aaaaatgaaa 300 gaagagtatg acaaaattca gattgctgac ttgatggaag aaaagttccc aggtgatgct 360 ggtttgggca aactaataga attcttcaaa gaaataccaa cactgggaga ccttgctgca 420 actcttaaaa gagaaaagtt aaaagttgtg aataaaattg aatccattcc agtcaaagga 480 acaaccccat ctaaaatgag gaaacagaaa gaagtggatc ctgctacacc tgcatccacc 540 acaagcaaca cactcacagc taaaggagca gaggagactc ttggacctca gaaaagaaga 600 aaaccatctg aagaagagac tggaaccaaa aggagtaaaa tgtccaaaga gcagactcag 660 ccttcctgct ctgcaaaagc cagcacgtcc acagccatgg gctgttcccc 710 // ID JK123758; SV 1; linear; mRNA; EST; MAM; 519 BP. XX AC JK123758; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB16FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-519 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 8ad5bfdf29baaab2a79ed63fcbe59679. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000007720 (XM_001117930.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0OTZ2I01DAPQ7, F0OTZ2I01DAPQ7, F0X19WM02HSJX8, F1HESOY02H8L7T CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:7027146-7027663. XX FH Key Location/Qualifiers FH FT source 1..519 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 519 BP; 77 A; 162 C; 183 G; 70 T; 27 other; gctctgccct gcaccctagg gctcgggatg ctgctggccc tgccaggggc cttgggctcg 60 ggtggcagcg cggaggacag cgtgggctcc agctctgtca ccgttgtcnn nnnnnnnnnn 120 nnnnnnnnnn nnnnngtcac cggcctggca ctggcctggc gccgcctcag ccgtgactca 180 gggggctact accacccggc ccgcctgggt gccgcgctgt ggggccgcac acggcgcttg 240 ctctgggcca gccccccggg ccgctggctg caggcccgag ctgagctggg gtccccagac 300 aatgaccttg agcgacagga ggatgagcag gacgcagact atgaccacgt cacggatggt 360 ggcctgcagg ctgaccccgg gaaaggcgag cagctatgtg gagaggcgtc cagcccagag 420 caggtccccg tgcgggctga ggaagccaga gacagtgaca cggagggcga cctggtcctt 480 ggctccccag gaccagcaag ctcagggggc agtgctgag 519 // ID JK123759; SV 1; linear; mRNA; EST; MAM; 425 BP. XX AC JK123759; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB18FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-425 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; e551ed0f97c47c0f8fc492f81bfa8137. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000035108 (LOC100397440) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000007017 CC High quality match with Homo sapiens transcript: ENST00000440407 CC (FBXL15-006) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000004247 (LOC713188) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000011886 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000019303 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000057445 (XM_001171119.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000003129 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1C5L3D02D4H3Z, F1C5L3D02D4H3Z, F0Z1IVZ01CEH8J, FZ8A1BE02GHHPH, CC F1GITI002HQ1PK CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874252|FR874252.1:102548737-102548956. XX FH Key Location/Qualifiers FH FT source 1..425 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 425 BP; 51 A; 133 C; 170 G; 71 T; 0 other; ccgatggagc caccgatgga gccgtccgga ggggagcaag agcccggagc cgtcaggctc 60 ctggacctgc cctgggaaga tgtgctgctc ccacacgtcc tgaaccgggt cccgctgtgc 120 cagctgctcc ggctgcagcg cgttagccgg gccttccggg cgctggtgca gcttcacctg 180 gccgggctgc gtcgcttcga tgcagcgcag gtgggtctgc agatcccgcg ggccgcattg 240 gcctggctgc tgcgggacgc cgaggggctg caggagctgg cgctggcgcc gtgtcacgaa 300 tggttgtcag acgaggacct ggtgccggtg ctggcgcgta atccgcagct gcggagtgtg 360 gcgctgggcg gttgcgggca actgagtcgt cgggcgcttg gggcgctggc cgagggctgc 420 ccacg 425 // ID JK123760; SV 1; linear; mRNA; EST; MAM; 670 BP. XX AC JK123760; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB19FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-670 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; c38b13e81ef033d1f052c7124a5f4269. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3641 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB02FP78E, F0Z8TLB02JHK5O, F0Z8TLB01DRE9J, F0Z8TLB01A3OM3, CC F0BOF8H01AJSA9 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:117850325-117850227. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 670 BP; 190 A; 143 C; 167 G; 141 T; 29 other; ggaatggtaa agtggcttgg ctttgagact ggagtgagac cccagcccta ggctggggtt 60 ctttccatta tagaggagac ggattcagaa gggctacaga ccaaggttat tgaaaaccag 120 acatatgatg aacgtctaga gattaacgac tctgaagagg ttgcaagtat ttatactcca 180 accccaagat accaaggact tcctcattct gcccatcttc ctaacaaggc tatggctgat 240 aacagcagtg atgagtatga agaggaaaat aacgaggtcc taagggaggg catgccacag 300 gctccaggcc acagaggcaa agacatggac cctgttccac ctgcccctga aagtctcaag 360 tgccaccaga ccccttctca tggacttgag agggtgggat ggtacagaga gagccagaag 420 cacacaaagg agaagaagaa gacctcacag ttgacacctc aacggggctt tagtgaaann 480 nnnnnnnnnn nnnnnnnnnn nnnnnnntca tctgaaactg attctgattc tgatgatgat 540 gatgaagagc atggagcccc tctggaaggg gcctatgacc ctgcagatta tgagcatttg 600 ccagtttctg ctgagattaa ggaactcttc cagtacatca gtaggtatac acctcagttg 660 attgacctgg 670 // ID JK123761; SV 1; linear; mRNA; EST; MAM; 710 BP. XX AC JK123761; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB20FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-710 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 282a059f144f9664dc1f49737f7796db. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3782 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000012746 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000034153 CC High quality match with Homo sapiens transcript: ENST00000360566 CC (RRM2-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000002909 (XM_001091447.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000012790 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000017579 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000007080 CC High quality match with Pongo abelii transcript: ENSPPYT00000014702 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB01DVSFN, F1RHTUQ01ALVA8, FZ8A1BE02F7LP4, FZ8FYPF01DTW0M, CC F1HESOY01A4AXS CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874264|FR874264.1:42250575-42249969. XX FH Key Location/Qualifiers FH FT source 1..710 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 710 BP; 178 A; 192 C; 189 G; 150 T; 1 other; ctgggctgta cttcgctgcg ccgccactat gctctccgtc cgcatcccgc tcgcgcccat 60 cacgaacccg cagcagctgc agctctcacc gctgaagggg ctaagcctgg tcgacaagga 120 gaacacgccg ccagccctga gcgggacccg cgtcctggcc agcaagaccg cgaggaggat 180 cttccaggag cccgcggagc cgaaaactaa agcagctgcc cccggcgtgg aggaggaacc 240 gctgctgaga gaaaaccccc gccgctttgt catcttcccc atcgagtacc atgatatctg 300 gcagatgtat aagaaggcgg aggcttcctt ctggaccgcc gaggaggtgg acctgtccaa 360 ggacattcag cactgggaat ccctgaagcc cgaggagaga tattttatat cccatgttct 420 ggctttcttt gcagcaagcg atggcatagt aaatgaaaac ttggtggagc gatttagcca 480 agaagttcag attacagaag cccgctgttt ctatggcttc caaattgcca tggaaaacat 540 acattctgaa atgtatagtc ttcttattga cacttacata aaagatccca aagaaaggga 600 atttctcttc aatgccattg aaacgatgcc ttgtgtcaag aagaaggcag attgngcctt 660 gcgctggatt ggggacaaag aggctaccta tggtgaacgt gtcgtagcct 710 // ID JK123762; SV 1; linear; mRNA; EST; MAM; 300 BP. XX AC JK123762; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB22FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-300 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; c906de9354b37fabc0f3c0818584bbfa. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000036633 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000010013 CC High quality match with Homo sapiens transcript: ENST00000471841 CC (MFN1-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000047904 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000014875 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000031892 (XM_527007.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000023298 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01DTVG5, F0Z8TLB02F06II, F0M2C4T01CK3EG, F0Z8TLB02I4M3I, CC F0X19WM01C26ZY CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874264|FR874264.1:155658572-155658385. XX FH Key Location/Qualifiers FH FT source 1..300 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 300 BP; 77 A; 76 C; 93 G; 54 T; 0 other; aaaccttcaa cagatcggcc gggcgtggtg gctcatacct gtaatcccag cactttggga 60 ggccgaggca ggtggatcac gaggtcggga gatcgagacc atcctggcta ccacggtgaa 120 atcccatctc tactaaaaat acagaaaaat tagccgggcg tggtggcggc tgcctgtagt 180 cccagctact cgggaggctg aggcaggaga atggcatgaa cctgggaggt ggagcttgca 240 gtgagctgag atcatgccac tgcactccag cctgagtgac agagcaagac tgcctcaaaa 300 // ID JK123763; SV 1; linear; mRNA; EST; MAM; 594 BP. XX AC JK123763; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB23FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-594 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; d3ee766d9ad5191ae68dbee24a707caa. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000009877 (LOC100407500) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000007130 CC High quality match with Homo sapiens transcript: ENST00000232496 CC (TUSC2-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000009771 (XM_001101682.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000008924 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02ILBSI, FZ8FYPF02JJ2SR, F0X5I7102IO5IB, F0ZWYBK01CABDV, CC F0M2C4T01EPAMT CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874245|FR874245.1:86378765-86378980. XX FH Key Location/Qualifiers FH FT source 1..594 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 594 BP; 114 A; 175 C; 203 G; 102 T; 0 other; gaaggcggcg gcggcggcgg cacctgcgag cagcgactag ggcaggttat ggtggagtgg 60 attgcgctgt gagcagagcg gccgcggggc ccgccatgcg ccggcggccc tgacatgggc 120 gccagcgggt ccaaagctcg gggcctgtgg cccttcgcct cggcggccgg aggcggcggc 180 tcagaggcag caggagctga gcaagctttg gtgcggcctc ggggccgagc tgtgcccccc 240 ttcgtattca cgcgccgcgg ctctatgttc tatgatgagg atggggatct cgctcacgag 300 ttctatgagg agacaatcgt caccaagaac gggcaaaagc gggccaagct gaggcgagtg 360 cataagaatc tgattcctca gggcatcgtg aagctggatc ccccccgcat ccacgtggat 420 ttccctgtga tcctctatga ggtgtgaccc tgggaggtgg cagacggaag caccccctgc 480 cccagcaaga aactcccagg ctcaatcaag gtgtgacttc cattgaggag cccaggctgg 540 ggccacaacc ctgaataaac tccattggcc cataaccttc agctgtgagc gggt 594 // ID JK123764; SV 1; linear; mRNA; EST; MAM; 734 BP. XX AC JK123764; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YB24FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-734 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1804c905c44921d0c105e94a043ce7b7. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2806 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000032609 (LOC100397693) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000012968 CC High quality match with Homo sapiens transcript: ENST00000269349 CC (EIF4A3-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000013384 (XM_001110130.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000009138 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000019870 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000017878 (XM_511724.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000010170 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0DTF6N02ILT76, F0ZWYBK02JCDNX, F1RHTUQ01EFWQW, F1RHTUQ02IAAF9, CC F0M2C4T02HVWTK CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:26618844-26618751. XX FH Key Location/Qualifiers FH FT source 1..734 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 734 BP; 188 A; 179 C; 211 G; 156 T; 0 other; gcagcgagag gagtcggaag gtgctcttcc gcgggctcta aatcatggcg accacggcca 60 cgatggcgac ctcgggctcg gcgcgaaagc gtctgctcaa agaggaagac atgaccaaag 120 tggaattcga gaccagcgag gaggtggatg tgacccccac gttcgacacc atgggcctgc 180 gggaggacct gctgcggggc atctacgctt acggttttga aaaaccatca gcaatccagc 240 aacgagcaat caagcagatc atcaaaggga gagatgtcat cgcgcagtct cagtccggca 300 caggcaaaac agccactttc agtatctcag tcctccagtg tttggatatt caggttcgtg 360 aaactcaagc tttgatcttg gctcccacaa gagagttagc tgtgcagatc cagaaggggc 420 tgctcgctct cggcgactac atgaatgtcc agtgccatgc ctgcattgga ggcaccaatg 480 ttggggagga catcaggaag ctggattatg gacagcatgt tgtcgcgggc actccagggc 540 gtgtttttga tatgattcgt cgcagaagct taaggacacg tgctatcaaa atgttggttt 600 tggatgaagc tgatgaaatg ttgaataaag gtttcaaaga gcagatctat gacgtgtaca 660 ggtacctgcc tccagccaca caggtggttc tcatcagtgc cacgctgcca cacgagattc 720 tggagatgac caac 734 // ID JK123765; SV 1; linear; mRNA; EST; MAM; 637 BP. XX AC JK123765; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC01FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-637 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; c361e729424862d827d9918f13a08334. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3026 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000063060 (B2MG_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000023544 CC High quality match with Homo sapiens transcript: ENST00000349264 CC (B2M-001) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000007249 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000012956 (B2MG_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000007591 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE02HIFBI, FZ8FYPF02HY8IJ, FZ8FYPF02HIYM6, FZ8FYPF02FN0ES, CC F0BWDJC02H5DLY CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:23282842-23283083. XX FH Key Location/Qualifiers FH FT source 1..637 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 637 BP; 167 A; 135 C; 150 G; 185 T; 0 other; gaggacagca cccgggccga gatgtctcgc tcagtggcct tagccgtgct ggcgctactc 60 tttctttctg gcctggaggc tatccagcgt gctccaaaga ttcaggttta ctcacgccat 120 ccaccagaga atggaaagcc aaatttcctg aattgctatg tgtctgggtt tcatccatct 180 gatattgaag ttgacttact gaagaatgga gagaaaatgg ggaaagtgga gcattcagac 240 ttgtctttca gcaaagactg gtctttctat ctcttgtact acactgaatt cacccccaat 300 gaaaaagatg agtatgcctg ccgtgtgaac cacgtgactt tgtcagggcc caggacagtt 360 aagtgggatc gagacatgta agcagcatca tggaggtttg aagatgccgc atttggattg 420 gatgaattcc aaattctgct tccttgcttt ttaatattga tatgcttata cactttatgc 480 aaaaaatgca gagttataat aatgttaaca caggcatgat cttctttata attctacttt 540 gagtgctatc tccacgtttg aggtatctgg gcctctagct ttaggagggc tcgcaactta 600 gaggtgggga gcagagaatt ctcatatcca acatcca 637 // ID JK123766; SV 1; linear; mRNA; EST; MAM; 560 BP. XX AC JK123766; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC02FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-560 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 9161c4d54a139a6038e7a07066e49e97. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RHTUQ01D3EEG, F01W12W02FE4CK, F0ZWYBK01C8IR0, F0OTZ2I02FRDSK, CC F0BOF8H02HSH8Z CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874259|FR874259.1:73715889-73716460. XX FH Key Location/Qualifiers FH FT source 1..560 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 560 BP; 128 A; 147 C; 141 G; 144 T; 0 other; tgcctccctt cccccagttg tgacaaaatt gtttctagtt gttgtgaaat gtcccctggg 60 tgggaatcga gaggggaaaa accctcctga gttgagaact ttgctcctta accctccaca 120 tgagggtaga aggcagaccc cagactctcc cacctcaggg tttcaggacg gaggaaggtc 180 ctcagccccc ctccacctgg cacttcctcc cccgtccagt gcagacgcca cccctctccc 240 aggtaagcat taaaccctaa ccccagcaga ggtccctggg caaccagggg ccttggagat 300 aaagatggag gcatccgagt tgtttgggac gtttcaaagt gggccctggt agtcatgctg 360 ttgctgttgg tggttttgtc ccttgtgttt aggggacatt atggtttgaa ttgtgtcccc 420 caccccacca aattcatatg ctgatgtcct aacccagaat atctctgaat gtgacctcat 480 ttggagataa ggtcttacaa agtggagtga tgggggtagg ttctaatcca atgactagtg 540 tccttattaa aagggaaatc 560 // ID JK123767; SV 1; linear; mRNA; EST; MAM; 666 BP. XX AC JK123767; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC03FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-666 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; edf23da7932d00d9aeaf8430166758d7. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2806 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000032614 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000012968 CC High quality match with Homo sapiens transcript: ENST00000269349 CC (EIF4A3-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000013384 (XM_001110130.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000009138 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000019870 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000017878 (XM_511724.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000010170 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0DTF6N02ILT76, F0ZWYBK02JCDNX, F1RHTUQ01EFWQW, F1RHTUQ02IAAF9, CC F0M2C4T02HVWTK CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:26618833-26618751. XX FH Key Location/Qualifiers FH FT source 1..666 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 666 BP; 171 A; 161 C; 189 G; 145 T; 0 other; atcatggcga ccacggccac gatggcgacc tcgggctcgg cgcgaaagcg tctgctcaaa 60 gaggaagaca tgaccaaagt ggaattcgag accagcgagg aggtggatgt gacccccacg 120 ttcgacacca tgggcctgcg ggaggacctg ctgcggggca tctacgctta cggttttgaa 180 aaaccatcag caatccagca acgagcaatc aagcagatca tcaaagggag agatgtcatc 240 gcgcagtctc agtccggcac aggcaaaaca gccactttca gtatctcagt cctccagtgt 300 ttggatattc aggttcgtga aactcaagct ttgatcttgg ctcccacaag agagttagct 360 gtgcagatcc agaaggggct gctcgctctc ggcgactaca tgaatgtcca gtgccatgcc 420 tgcattggag gcaccaatgt tggggaggac atcaggaagc tggattatgg acagcatgtt 480 gtcgcgggca ctccagggcg tgtttttgat atgattcgtc gcagaagctt aaggacacgt 540 gctatcaaaa tgttggtttt ggatgaagct gatgaaatgt tgaataaagg tttcaaagag 600 cagatctatg acgtgtacag gtacctgcct ccagccacac aggtggttct catcagtgcc 660 acgctg 666 // ID JK123768; SV 1; linear; mRNA; EST; MAM; 697 BP. XX AC JK123768; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC04FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-697 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 332ebbd9cc1e39840b38696ff889fa48. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3642 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000027097 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H02JLRZ2, F0DTF6N01DG0A8, F1GITI001EXAXN, F0OTZ2I02HK0JW, CC F0X19WM02GF802 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:170085137-170084811. XX FH Key Location/Qualifiers FH FT source 1..697 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 697 BP; 131 A; 220 C; 206 G; 140 T; 0 other; tgcccatgca caggcccgat gtctacttgc tgccaccagc ccgggagcag ctgaacctgc 60 gggagtcggc caccatcacc tgcctggtga cgggcttctc tcccgcggac atcttcgtgc 120 agtggatgca gagggggcag cctttgtccc cagagaagta tgtgaccagt gccccaatgc 180 ctgagcccca ggccccaggc cggtacttcg cccacagcat cctgaccgtg tctgaagagg 240 actggaacac gggggagacc tacacctgcg tcgtggccca cgaggccctg cctaacaggg 300 tcaccgagag gaccgtggac aagtccaccg agggggaggt gagtgctgac gaggagggct 360 ttgagaacct gtgggccact gcctccacct tcatcgttct cttcctcttg agcctcttct 420 acagtaccac cgtcaccttg ttcaaggtga aatgatccca acagaagaac atcggagacc 480 agagacagga actcaaaggg gcgctgcctc cgggtctggg gtcccggcct gtgtggcctg 540 ttggcgcgtg tttctcttcc ctgcccggcc tccagctatg tgctctaaca caggcttcct 600 tctagagagg caggggcggg ctggtgtgcg gaccacgacg ggggctcccc tccacatcgc 660 tttgctgtgt atacccttgt tgccctgaaa taaatat 697 // ID JK123769; SV 1; linear; mRNA; EST; MAM; 684 BP. XX AC JK123769; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC05FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-684 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 211d67acd1f0334301ecbd84f5688808. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3643 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000004397 (CTSH) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000012331 CC High quality match with Homo sapiens transcript: ENST00000220166 CC (CTSH-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000014095 (XM_001108862.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000011249 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000013560 (XM_001153217.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0DTF6N02JYST5, F0BOF8H01EL6UM, F1GITI002GDJOE, F1C5L3D04IS92H, CC F0M2C4T02IGGJ8 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:58434562-58434427. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 684 BP; 173 A; 199 C; 180 G; 132 T; 0 other; acctccgtgg accctgagcg ccagagccca gccgccaccg ccgcgatgtg ggtcacgctg 60 ccgctgctct gcgccggagc ctggctcctg ggagcccccg tctgcggcgc cgcggaactg 120 tccgtgaact ccttagagaa gtttcacttc aagtcatgga tgtctaagca ccataagaca 180 tacagcacgg aggagtacca ccacaggatg cagacgtttg ccagcaactg gaggaagata 240 aatgcccaca acaatgggaa ccacacattt aaaatggcac tgaaccaatt ttcagacatg 300 agctttgctg aaataaagca caagtatctt tggtcagagc ctcagaactg ctcagccacc 360 aaaagtaact accttcgagg tactggtccc tacccacctt ccatggactg gcggaaaaaa 420 ggaaattttg tctcacctgt gaaaaatcag ggtgcctgcg gcagttgctg gactttctcc 480 accacagggg ccctggagtc tgcgattgcc atcgcaaccg ggaagatgct gtccttggct 540 gaacagcagc tggtggactg cgcccaggac ttcaacaatc acggctgcca agggggtctc 600 cccagccagg ctttcgagta catcctgtac aacaagggga tcatgggtga agacacctac 660 ccctaccagg gcaaggatgg tgat 684 // ID JK123770; SV 1; linear; mRNA; EST; MAM; 249 BP. XX AC JK123770; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC06FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-249 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 32366a3b107a072360d805290ba0262c. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1200 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB01A5Q7N, F1RHTUQ01B5WPY, F1RTSFR02F6NCO, F0M2C4T01BSCJ0, CC F0M2C4T01C7C5A CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:29701957-29702147. XX FH Key Location/Qualifiers FH FT source 1..249 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 249 BP; 69 A; 35 C; 50 G; 95 T; 0 other; agattccatt ttatggagag agagcttaag tgaaggcagt ttttatggcg ttttgtagta 60 aattctgtaa aatactttgg tttaatttgt gacttttttt gtaagaaatt gtttataata 120 tgttgcattt gtacttaagt cattccatct ttcactcagg atgaatgcga aaagtgactg 180 ttcacagacc tcagtgatgt gagcagtcac ttttcgcatt catccttgta ctaagttggc 240 attacaaga 249 // ID JK123771; SV 1; linear; mRNA; EST; MAM; 302 BP. XX AC JK123771; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC07FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-302 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 7ec4f06e6360d655abf0a4d12c9ad181. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1572 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000041261 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000011754 CC High quality match with Homo sapiens transcript: ENST00000353334 CC (CD74-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000012750 (XM_001099491.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000013239 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000004631 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000032219 (XM_001166096.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000018564 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RTSFR01ATCF7, FZ8FYPF02IBEXR, FZ8A1BE02JJGY2, FZOOKC302JQSQS, CC F0BWDJC01AYYX8 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874249|FR874249.1:147780616-147780564. XX FH Key Location/Qualifiers FH FT source 1..302 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 302 BP; 76 A; 87 C; 87 G; 52 T; 0 other; tgcgcatggc aaccccgctg ctgatgcagg cgctacccat gggagcccag gggcccatgc 60 agaatgccac caagtatggc aacatgacgg aggaccatgt gatgcacctg ctccagaatg 120 ctgaccccct gaaggtgtac ccgccgctga aggggagctt cccggagaac ctgagacacc 180 ttaagagcac catggagacc ttggactgga aggtctttga gagctggatg caccattggc 240 tcttgtttga aatgagcaag cactccttgg agcaaaagcc cactgaggct cccccaaaag 300 ag 302 // ID JK123772; SV 1; linear; mRNA; EST; MAM; 494 BP. XX AC JK123772; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC08FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-494 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; bd0a056bd5336392e5046736d4c73ee4. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01CC98A, FZ8FYPF02FK84C, F1HESOY01D58VY, F1HESOY02HKUMQ, CC F0BOF8H02I4EX5 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874251|FR874251.1:148304607-148304876. XX FH Key Location/Qualifiers FH FT source 1..494 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 494 BP; 135 A; 115 C; 135 G; 109 T; 0 other; tgttagcatc atgctctaac caaggaagct tagtgttccc cgcagtgctc tgtatctggc 60 ctgggtctcc tcaagcggca gcccccactg tccagcatgt agaaggtaga ttcttatgac 120 aacaccagac ccatagttac caggaaacaa aaaggaagct gagtattgtg aaggggaaga 180 aatccttttt ataagaagga atgattttta ggccgggtgc agtgactcat gcctgcaatc 240 ccagcacttt gggaggctga gacaggcaga tcacttgagg tcaggagttt gagacctgcc 300 tggccaacat gatgaaaccc tgtctctact aaaaatgcaa aaaaattagc caggcatggt 360 ggcatgtgcc tgtagtccca gctactgggg aggctgaggc aggagaatca cttgaatctg 420 ggaggcggag gttgcagtga gctgagatca caacactgca ctccagcctg ggtgacagag 480 cgagactcca tctc 494 // ID JK123773; SV 1; linear; mRNA; EST; MAM; 669 BP. XX AC JK123773; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC09FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-669 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 53b62fe203d680e6f108688d5012c502. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2122 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000010147 (LOC100401600) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000010724 CC High quality match with Homo sapiens transcript: ENST00000329970 CC (GTF3C6-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000017817 (XM_001087346.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000016396 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000034188 (XM_518690.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1C5L3D02D7CGY, F1C5L3D02D43W0, F0X19WM02GQLNB, F1RHTUQ02HS4YR, CC F0OTZ2I02HIY6D CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874264|FR874264.1:107091412-107092074. XX FH Key Location/Qualifiers FH FT source 1..669 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 669 BP; 230 A; 100 C; 160 G; 179 T; 0 other; ggagaagagg aggaagagga ggagcagttg gttctggtgg aattatcagg aattattgat 60 tcagactttc tctcaaaatg tgaaaataaa tgcaaggttt tgggcattga cactgagagg 120 cccattctgc aagtggacag ctgtgtcttt gctggggagt atgaagatac tcttgggacc 180 tgtgttatat ttgaagaaaa tgttgaacat gctgatacag aaggcaataa taaaacagtg 240 ctaaaatata aatgtcatac aatgaagaag ctcagcatga caagaactct tctgacagag 300 aagaaggaag gagaagaaaa cataggtggg gtggaatggc tgcaaataaa ggataatgat 360 ttctcctatc gacccaacat gatttgtagc tttctacatg aaaatgaaga cgaagaagtg 420 gcagcttcag ccccagataa atctttggaa ttggaagagg aagagattca aatgaacgac 480 agttcaaacc tgagttgtga acaggagaaa ccaatgcact tggatatgga agattctggt 540 cctcttattg atataccttc tgagacagaa ggttctgttt ttatggaaac tcaaatgctg 600 ccttagaaat cactcctaga tgaaattttt ctcataataa cttgtcaaga actttttaga 660 gttcttaca 669 // ID JK123774; SV 1; linear; mRNA; EST; MAM; 659 BP. XX AC JK123774; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC10FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-659 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 127f760e076ac70a03609e62b39ed1e1. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig225 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000015309 (LOC100397100) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000024126 CC High quality match with Homo sapiens transcript: ENST00000295702 CC (SSR2-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000018942 (XM_001113529.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000015697 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000002654 (XM_001162990.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Q2XTE02ISZMG, F0OTZ2I01EIDQ1, F0BOF8H02IQC23, F1IUBWX01EFCCB, CC F0BOF8H01EPRMH CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:135341682-135341527. XX FH Key Location/Qualifiers FH FT source 1..659 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 659 BP; 147 A; 179 C; 170 G; 163 T; 0 other; gaaaggcatt tgggatgccc acgatgaggc tgctggcgtt tgtggtgttg gctctatttg 60 ctgtcactca agcagaggaa ggagccaggc ttttggcttc caaatcactg ctgaacagat 120 atgccgtgga gggacgagac ctgaccttgc agtacaatat ctacaatgtc ggctcaagtg 180 ctgcattaga cgtggaacta tctgatgatt ccttccctcc agaagacttt ggcattgtgt 240 ctggaatgct caatgtcaaa tgggaccgga ttgcgcctgc tagcaatgtc tcccacactg 300 tggtcctgcg ccctctcaag gctggttatt tcaacttcac ctcggcgaca attacttacc 360 tggcccagga ggatgggccc gttgtgattg gctctaccag tgcacctgga cagggaggaa 420 tcctggctca gcgggagttt gacaggcgat tctcccctca ttttctggac tgggcagcct 480 ttggggtcat gacccttccc tccatcggca tccccctgct attgtggtac tccagcaaga 540 ggaaatatga cactcccaaa acaaagaaga actgagtggg gcttccacag ccctcctctc 600 ccaagaaatc caggctcctt tcccaagaaa tccaggtgct ttccagactc cagagggta 659 // ID JK123775; SV 1; linear; mRNA; EST; MAM; 669 BP. XX AC JK123775; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC11FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-669 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2b26cd19e4b4c9d21e71d4f6a9f63907. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000012585 (IDO1) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000027471 CC High quality match with Homo sapiens transcript: ENST00000518237 CC (IDO1-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000027111 (NM_001077483.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000015986 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000037404 (XM_519723.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000021618 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T02JO9GX, F0DTF6N02FI72N, F0Z8TLB02F1BII, F0Z8TLB01C07HO, CC F0M2C4T01CVV29 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874251|FR874251.1:40510245-40510315. XX FH Key Location/Qualifiers FH FT source 1..669 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 669 BP; 199 A; 143 C; 152 G; 175 T; 0 other; ataagatgct ctgaaaactc ttcaaacact gaggggcacc agaggagcag actacaagaa 60 tggcacatgc tatggaaaac tcctggacaa tcagtgaaga gtaccatatt gatgaagaag 120 tgggcttcgc tctgccaaat ccacaggaaa atctacctga tttttataat gactggatgt 180 tcattgctaa acatctgcct gatctcatag agtctggcca acttcgagaa agagttgaga 240 agttagacat gctcagcatt gatcatctca cagaccacaa gtcacagcgc cttgcacatc 300 tagttctggg atgcatcacc atggcatatg tgtgggataa aggtcatgga gacgtccgta 360 aggtcttgcc aagaaatatt gctgttcctt actgccaact ctccaagaaa ctgggactgc 420 ctcctattct ggtttatgca gactgtgtct tggcaaactg gaagaaaaag gatcctaata 480 agcccctgac ttatgagaac atggacgttt tgttctcatt tcgtgatgga gactgcagta 540 aaggattctt cctggtctct ctattggtgg aaatagcagc tgcttctgct atcaaagtaa 600 ttcctactgt attcagggca atacaaatgc aagaacggga cactctgcta aaggcactgt 660 tggaaatag 669 // ID JK123776; SV 1; linear; mRNA; EST; MAM; 639 BP. XX AC JK123776; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC12FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-639 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1d5bb93df88e28578e91259ac95a2ed8. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000002315 CC High quality match with Homo sapiens transcript: ENST00000527401 CC (PHF21A-003) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000006665 (XM_508394.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Q2XTE02JYOJT, F1C5L3D03G2623, F0BOF8H02IGZ5Y, F0Z8TLB02JZHTV, CC F1IUBWX01EV5T7 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:26616081-26616740. XX FH Key Location/Qualifiers FH FT source 1..639 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 639 BP; 109 A; 197 C; 178 G; 155 T; 0 other; ggctcaggcc caataggcag tgtgcatggg ggcggttgca cacagctgtt tccctgaggg 60 tgaccgtgtc tgcagcgcac ttctgtctcc gcatgcacgg ctgcctctcc agccacctct 120 gatacttctc tcggggccat cagagcctcc cttgggctct cacctctcag ctcacacact 180 cttcgtggtt tcctcccttc attcccttac agggcgtggt ccttcttact tatctaaagg 240 gctgaattta ggagactttt tacccagggg caaaaggctc ctagggtaat gggatggatg 300 gtggcccagg tgcattttcc agggcctggg ttctccagat cccgtggcgt ctgttgagtg 360 gaggcaactt tgctctgtgt gaacctcgcc cctgtccctc ggctgggtgc tcctgtccct 420 cggccgggca cccttggcag gaagcaggac tcccattctc accctaactt agactgtcct 480 ctgagtcagc tcctctccaa gacgggagtg ggcggccctg ggcagtcttc tggccccctt 540 gctaaagtga gaggcaggaa gctggggctg ccctccagac agccagggta ggaactctga 600 aaaatagctc ctctaaatgg aagcagggct ccccagttc 639 // ID JK123777; SV 1; linear; mRNA; EST; MAM; 406 BP. XX AC JK123777; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC14FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-406 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 72b80d06d33d69f08faa9e2fff47e1dd. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000020151 (IREB2) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000015714 CC High quality match with Homo sapiens transcript: ENST00000258886 CC (IREB2-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000033025 (XM_001107837.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000011048 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000013538 (XM_523125.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01DN2TY, F0M2C4T01E4RLA, F0M2C4T01BJB0A, F1RHTUQ02JZ59U, CC F1C5L3D02ETP71 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:57892663-57893066. XX FH Key Location/Qualifiers FH FT source 1..406 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 406 BP; 129 A; 62 C; 88 G; 127 T; 0 other; aagtattcag cgtgattgct tcgtttgaag atgatgtgga aataacattg tacaaacatg 60 gaggattatt aaactttgtg gcacgaaaat tctcatagta tctacttacc atggatacct 120 ttcataactg ataactgcaa agccttttgt gctggaccca ggaatcctta ccatggagct 180 gcagatagtc ccagtatact cacttatctc atccatggat gtaaatgatg atgaatcaac 240 atagtaactg aaatgaaatc ttcttgattt taaataatat acgaatggtg ctattaacat 300 tgctaaaatc aacgtgtgaa gtgtgttgtg gaagagacct gtaagtatgg ggggggggcg 360 atattttatc agaccatttt gtaaataaag gcagaatttg tgttgg 406 // ID JK123778; SV 1; linear; mRNA; EST; MAM; 684 BP. XX AC JK123778; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC17FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-684 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; a76ab0b38392694da031adae991ff5ff. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2429 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000057643 (TM9SF1) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000016135 CC High quality match with Homo sapiens transcript: ENST00000530611 CC (RP11-468E2.1-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000017519 (XM_001112694.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000001723 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000019559 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000010657 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000011382 (XR_022113.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000006724 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000002687 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB01CA3ZM, F0Q2XTE02FP1DD, F0Q2XTE02H21CR, F0DTF6N02GJAWA, CC F0BOF8H01ADJ9U CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:87593564-87593151. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 684 BP; 121 A; 182 C; 186 G; 195 T; 0 other; gccccacagc ttggatgggt tacgacctga tgagttccta ggccttaccc acacttatag 60 cgtgcgctgg tctgagactt cagtagagcg tcggagtgac aggcgccgtg gtgatgatgg 120 tggtttcttt cctcgaacac tggaaatcca ttggttgtcc atcatcaact ccatggtgct 180 tgtgttttta ctggtgggtt ttgtggctgt cattctaatg cgtgtgcttc ggaatgacct 240 ggctcggtac aacttagatg aggagaccac ctctgcaggt tctggtgatg actttgacca 300 gggtgacaat ggctggaaaa ttatccatac agatgtcttc cgcttccccc cataccgtgg 360 tctgctctgt gctgtgcttg gcgtgggcgc ccagttcctg gcccttggca ctggcattat 420 tgtcatggca ctgctaggca tgttcaatgt gcaccgtcat ggggccatta actcggcagc 480 catcttgttg tatgccctga cctgctgcat ctctggctac gtgtccagcc acttctaccg 540 gcagattgga ggcgagcgtt gggtgtggaa catcattctc accaccagtc tcttctctgt 600 gcctttcttc ctgacgtgga gtgtggtgaa ctcagtgcac tgggccaatg gttcgacaca 660 ggctctgcca gccacaacca tcct 684 // ID JK123779; SV 1; linear; mRNA; EST; MAM; 694 BP. XX AC JK123779; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC18FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-694 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 181a903175679bb77da9eecb72438f91. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX01DZSN1, F1HESOY02HUVDV, F0M2C4T02FQ5PT, F0DRJ6O02IFGTB, CC F0OTZ2I02IOZYW CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874264|FR874264.1:43777695-43776998. XX FH Key Location/Qualifiers FH FT source 1..694 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 694 BP; 182 A; 160 C; 133 G; 219 T; 0 other; ggttgtcttt tccttgtggt agaaaagaca aatgccaatt ttgaaatctt gaatgcagta 60 ggcaattgtg ttgaaatagt atgtgagaat tccttgcaag ctgaacatcc aggtctgggg 120 catgaacctg gcatacaatg tagatttctg tgttcgttag gcaacagcta cattgtctgc 180 tgggtttcag gctacctgga aacatgttct ccattggctg tctcaccgat gcttcaccta 240 ctcctataat gtttctgaat aactgttttc caggaggtat aaaccagtgg agtttatcat 300 tgaatagagt acagagaaag caccgcaaga aagctgatca cctcagcaat gaacgatttc 360 agtatgattt caccccagtg gtctcacaca ccagcctagt aaaccagaga taaggtgtaa 420 tgtcatcctt ccttctccct gcctcaactg tcaaagattg accgcttaca tgttgtttac 480 tttttggctt atttctgttc atagttatta tcagaaggca tagcacccac gagcctaatc 540 gtgctgcctt acaaccttat tcctcttcat tttgtcttct cacttacctg cagaaaccca 600 gtttattttt gttctgccct taccacacag ccttgtaact gttatgaatg accctcagtc 660 tcactggaat ctgaatcact caggaattta ggca 694 // ID JK123780; SV 1; linear; mRNA; EST; MAM; 640 BP. XX AC JK123780; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC19FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-640 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 4c48d82fb781382f1123d1b857927ccd. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01BPVIP, F0DRJ6O01B0KUL, F0X5I7102GPY59, F1GITI001D2GAD, CC F0M2C4T02IF70K CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:167591975-167591931. XX FH Key Location/Qualifiers FH FT source 1..640 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 640 BP; 198 A; 132 C; 130 G; 153 T; 27 other; atcctgtcca ggccactgct tcccaaggaa gtgcgactaa gcccatcagt gtatcaggta 60 atctatttag ataataccta gcctgatatg ttctaagcac tatgcnnnnn nnnnnnnnnn 120 nnnnnnnnnn nnaaattgag acagagtctc actctgcact ccagcctggg caacagagcg 180 agactccgtc tcaaaaaaaa aaaaaaaaag attacgttcc aagaatattt ggttgaaagc 240 ctattttcta caagcacaga gtttgtagaa ggtagacaat gtgacccaag gtgggtcact 300 aaagagctta ctttcttcaa cggaagggtg acccatgaaa acgttttcag ccctaaaatt 360 atatgaatta taggaatcta atatgtctac aggtctactt gccctatcca aaaggattct 420 gcatctggaa gaacagactg cccccagaat cctgtcacct ctgtcatcaa ccaatggtat 480 tcaatgctct ctaaacctaa aggaaagtat ctccacaaac tctcgtcttt ggggaaattc 540 ctagataatt cagggagctg agtaaattgc tctgtgtagg actaggaggc ttactcctgt 600 gaagtagggc cagagatgaa agagcaggga aaggaaaggt 640 // ID JK123781; SV 1; linear; mRNA; EST; MAM; 607 BP. XX AC JK123781; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC20FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-607 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 204c4e592620c30ad5b902a058a8234b. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3484 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000007552 (LOC100415563) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000023202 CC High quality match with Homo sapiens transcript: ENST00000451311 CC (TMSB4X-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000020004 (XM_001095905.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000055075 (XM_001174196.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X5I7101DYL68, F0M2C4T02H424B, FZ8FYPF02HTBOP, FZ8FYPF02F0S1B, CC FZOOKC302FJYVS CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874264|FR874264.1:114936932-114937471. XX FH Key Location/Qualifiers FH FT source 1..607 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 607 BP; 178 A; 127 C; 143 G; 159 T; 0 other; gctgcgcaga ccagacttcg ctcgttttcg tgcgcctcgc tccgcttttc ctccgcaacc 60 atgtctgaca aacccgatat ggctgagatc gagaaattcg ataagtcgaa actgaagaag 120 acagagacgc aagagaaaaa tccactgcct tccaaagaaa cgattgaaca ggagaagcaa 180 gcaggcgaat cgtaatgagg cgtgcgccgc caatatgcac tgtacattcc acaagcattg 240 ccttcttatt ttacttcttt tagctgttta actttgtaag atgcaaagag gttggatcaa 300 gtttaaatga ctgtgctgcc cctttcacat caaagaacta ctgacaacga aggccgcgcc 360 tgcctctccc atctgtctat ctggctggca gggaaggaaa gaacttgcat gttggtgaag 420 gaagaagtgg ggtggaagaa gtgggggtgg gacgacagtg aaatctagag taaaaccaag 480 ctggcccaag gtgtcctgca ggctgtaatg cagtttaatc agagtgccat tttttttttt 540 gttcaaatga ttttaattat cggaatgcac aatttttttt atatgcaaat aaaaagttta 600 aaaactt 607 // ID JK123782; SV 1; linear; mRNA; EST; MAM; 83 BP. XX AC JK123782; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC21FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-83 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 787633a962d9da4f0bb07ff539e628f5. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3074 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000024057 (LOC100411901) CC High quality match with Homo sapiens transcript: ENST00000491306 CC (RPL37A-001) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000047247 (XM_516077.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF02F9RU0, F1GITI001B1MRF, F0Z8TLB02HOXGB, F1IUBWX02JAPUF, CC F0M2C4T02G2TVJ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874255|FR874255.1:80013558-80013640. XX FH Key Location/Qualifiers FH FT source 1..83 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 83 BP; 29 A; 14 C; 16 G; 24 T; 0 other; aggagttgaa agaccagtag acgctcttct actctttgag acatcactgg cctataataa 60 atgggttaat ttatgtaaca aaa 83 // ID JK123783; SV 1; linear; mRNA; EST; MAM; 664 BP. XX AC JK123783; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC22FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-664 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 3e446f942d3718ce51e90e3d4df37e8a. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig302 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000022994 (B0KWW6_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000000131 CC High quality match with Homo sapiens transcript: ENST00000424325 CC (RPL10-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000007732 (LOC709376) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000004657 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000023847 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000011015 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000041869 (LOC739064) CC High quality match with Pongo abelii transcript: ENSPPYT00000024341 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000004067 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE01DL15F, F0Q2XTE02IFUTZ, F0BOF8H02HMJY6, F0BOF8H01EUE0Y, CC F0Z8TLB02IRLFT CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:170943546-170944208. XX FH Key Location/Qualifiers FH FT source 1..664 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 664 BP; 151 A; 177 C; 188 G; 148 T; 0 other; cctggtgtcg ccatgggccg ccgccccgcc cgttgttacc ggtattgtaa gaacaagccg 60 tacccaaagt ctcgcttttg ccgaggtgtc cctgatgcca agattcgcat ctttgacctg 120 gggcggaaga aggcaaaagt ggatgagttt ccgctctgtg gccacatggt gtcagatgaa 180 tatgagcagc tgtcctctga agccctggag gctgcccgaa tttgtgccaa taagtacatg 240 gtaaaaagtt gtggcaagga tggcttccat atccgggtgc ggctccaccc cttccacgtc 300 atccgcatca acaagatgtt gtcctgtgct ggggctgaca ggctccaaac gggcatgcga 360 ggtgcttttg gaaagcccca gggcaccgtg gccagggttc acattggcca agttatcatg 420 tccatccgca ccaagctgca gaacaaggag catgtgattg aggccctacg cagggccaag 480 ttcaagtttc ctggccgcca gaagatccac atctcaaaga agtggggctt caccaagttc 540 aatgctgatg aatttgaaga catggtggct gaaaagcggc tcatcccaga tggctgtggg 600 gtcaagtata tcccccaatc gtggtcctct ggacaaatgg cgagccctgc actcatgagg 660 gctt 664 // ID JK123784; SV 1; linear; mRNA; EST; MAM; 80 BP. XX AC JK123784; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC23FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-80 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; e94f96d950eec1f005a6b83656ba7a09. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3621 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000057896 CC High quality match with Homo sapiens transcript: ENST00000339399 CC (ANP32B-001) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000021600 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000039151 (XM_001156957.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RTSFR01ETEQI, F1IUBWX02IXWPH, F0X5I7101A4Z1O, F0X19WM02HMQ3K, CC F0OTZ2I01A7CKM CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874264|FR874264.1:78125855-78125776. XX FH Key Location/Qualifiers FH FT source 1..80 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 80 BP; 21 A; 14 C; 20 G; 25 T; 0 other; atgattaaga ccccagatga cctgcagaaa cagaactgtt cagtattggt tggactgctc 60 atggatttgg tagctgtttt 80 // ID JK123785; SV 1; linear; mRNA; EST; MAM; 715 BP. XX AC JK123785; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YC24FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-715 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 4acd6d240a966cf5f18515895aee88bd. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2649 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000003888 CC High quality match with Homo sapiens transcript: ENST00000395042 CC (RGS19-001) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000013089 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000045822 (XM_514823.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1C5L3D02EYLYU, F0X5I7101DCFGV, FZ8A1BE02HP7FV, FZ8A1BE02IKDMI, CC F0DRJ6O02GY3NL CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874253|FR874253.1:266036-266507. XX FH Key Location/Qualifiers FH FT source 1..715 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 715 BP; 129 A; 235 C; 228 G; 123 T; 0 other; gtgaagtatg tgccacgcca agtcctgagg aggtgcagag ctgggcccag tcttttgaca 60 agctgatgca cagcccagcg ggacgcagcg tgttccgggc gttcctgcgg acagagtaca 120 gcgaggagaa catgctcttc tggctggcct gtgaggagct gaaggccgag gccaaccagc 180 acgtggtgga cgagaaggcg aggctcatct acgaggacta cgtgtccatc ctgtccccca 240 aggaggtgag cctggactcc cgtgtgcggg agggcatcaa caagaagatg caggagccat 300 ccgcacacac gtttgacgac gcacagctgc agatctacac actcatgcac cgggactcct 360 acccacgctt tctcagctct cccacctacc gtgccctgct gctgcagggg ccgtcacagt 420 cctcctccga ggcctaggcc gcccccagca gcgcagaccc caccgcctcc tatggccgac 480 tctgggttgc cttcaggtgt tggctgtcgg ggcacacacc tgcaggccca gcctggccgg 540 ctggggtccc cacccatgga gacggtccta gacccagtgg ggagtgctgt gcctcctggc 600 cctgcccacc cgtggccaag caggagctcc aggtgcaggg tgggccagaa gccttctcat 660 cggcccgcag ctggctggcc cagtgctcct ggtgggggct cttgcatggt gagag 715 // ID JK123786; SV 1; linear; mRNA; EST; MAM; 481 BP. XX AC JK123786; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD01FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-481 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 6d0c72b5b60fce6742c2f6a95f395c32. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1303 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000009179 CC High quality match with Homo sapiens transcript: ENST00000312648 CC (CD7-201) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000018052 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000017990 (XM_001167912.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000010241 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BWDJC01B2ZQ9, FZ8A1BE01AVJD5, F0BOF8H02GAZOX, F0X5I7101CK0VJ, CC F1HESOY01ECHES CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874259|FR874259.1:78005898-78005642. XX FH Key Location/Qualifiers FH FT source 1..481 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 481 BP; 88 A; 175 C; 135 G; 83 T; 0 other; ggtgtgggga acatggctgg gcccccgagg ctcctgctgc tgcccctcct cctggcgctg 60 gctcatggcc tgcctggggc cctggctgcc caagaggtgc agcagtctcc ccactgcacg 120 attgcccccg tgggaggctc cgtcaacatc acctgctcca ccagcgggga cctgcatggg 180 atctacctga ggcagctcgg gccacagccc caaaacatca tttactacga ggacggggtg 240 gtgcccacta cggacaaacg gttccagggc cgcattgact tctcggggtc ccaggacaac 300 ctgactatta ccatgcacca cctgcagccg tcggacactg gcacctacac ctgccaggcc 360 atcacggaga tcaatgtcta cggctccggt accctggtcc tggtgacaga ggaacaatcc 420 caaggattgc acagatgctc cgacgccccg ccgacaggct ctgccctccc tgtccccgcc 480 g 481 // ID JK123787; SV 1; linear; mRNA; EST; MAM; 490 BP. XX AC JK123787; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD02FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-490 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 5fb03a46f95eaf3759241f1abca1724d. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1145 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000022649 (B0VX78_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000028499 CC High quality match with Homo sapiens transcript: ENST00000320868 CC (HBA1-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000000859 (HBA_MACMU) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000010382 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000010223 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000002534 CC High quality match with Pongo abelii transcript: ENSPPYT00000008138 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB01EFJPF, F0DTF6N01BZQPC, F0M2C4T01BK5LM, F0DRJ6O02GRH4D, CC F0Z8TLB01EFJPF CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874263|FR874263.1:133866-134041. XX FH Key Location/Qualifiers FH FT source 1..490 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 490 BP; 83 A; 179 C; 141 G; 87 T; 0 other; cagaaagaac ccaccatggt gctgtctcct gccgacaaga gcaacgtcaa ggccgcctgg 60 ggtaaggtcg gcggccacgc cggcgagtat ggtgcggagg ccctggagag gatgttcctg 120 tccttcccca ccaccaagac ctacttcccc cacttcgacc tgagccacgg ctctgcccag 180 gttaagggcc acggcaagaa ggtggccgac gcgctgaccc tcgccgtggg gcacgtggac 240 gacatgcccc acgcgctgtc cgcgctgagc gacctgcacg cgcacaagct tcgggtggac 300 ccggtcaact tcaagctcct gagccactgc ctgctggtga ctctggccgc ccacctcccc 360 gccgagttca ctcctgcggt gcacgcctcc ctggacaagt tcctggcttc tgtgagcacc 420 gtgctgacct ccaaataccg ttaagctgga gcctcggtgg ccatgcttct tgccccttgg 480 gcctcccgca 490 // ID JK123788; SV 1; linear; mRNA; EST; MAM; 705 BP. XX AC JK123788; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD03FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-705 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; d03672a18c403679b92ce2d99eb7cda1. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig292 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000025510 (EIF2B5) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000007463 CC High quality match with Homo sapiens transcript: ENST00000544027 CC (EIF2B5-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000009193 (XM_001104122.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000007788 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000029232 (XM_001139351.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BWDJC02GCASN, FZ8FYPF02FJSD4, F0Z8TLB02F7KUZ, F0Z8TLB02FEAWV, CC F1RHTUQ01EKSUC CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874245|FR874245.1:102921848-102921754. XX FH Key Location/Qualifiers FH FT source 1..705 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 705 BP; 183 A; 175 C; 188 G; 159 T; 0 other; gtgttccaga atgaagttct aggaacacta cagcggggca aagaggagaa catttcttgt 60 gacaatctcg tcctggaaat caattctctc aagtatgcct ataacataag tctaaaggag 120 gtgatgcagg tactgagcca cgtggtcctg gagttccccc tgcaacagat ggattccccg 180 cttgactcaa gccgctactg tgccctgctg cttcctctgc tcaaggcctg gagccctgtt 240 ttcaggaact acataaagcg tgcagccgac cacttggaag cgttagcagc cattgaggac 300 ttcttcctag agcatgaagc tcttggtatt tccatggcca aggtactgat ggctttctac 360 cagctggaga tcctggctga ggaaacaatt ctgagctggt tcagccaaag agatacaact 420 gacaagggcc ggcagttgcg caagaatcaa cagctgcaga ggttcatcca gtggctaaaa 480 gaggcagaag aggagtcatc tgaagatgac tgaagtcaca ctgcctgccc ctgtgggtgt 540 gattgagtac cctcctggct cctgggctgg gacaagtaag gaactagctg cagaaggatg 600 aatgaccacc atccaggctg agactgaaag gagcagaggc tggaactaca gtattctctc 660 ccctgctagc aaccatgtgc ctcccatcct gactgtggaa ttggg 705 // ID JK123789; SV 1; linear; mRNA; EST; MAM; 615 BP. XX AC JK123789; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD05FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-615 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 510c1c7f97b2a7485e86979e1f466d88. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Q2XTE01BGMU7, F0Z8TLB01CPEKQ, F0ZWYBK02FWU8L, FZ8A1BE02IWQ4D, CC F0X5I7101AYKJG CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/). XX FH Key Location/Qualifiers FH FT source 1..615 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 615 BP; 129 A; 143 C; 210 G; 133 T; 0 other; ggaatgtggg ccctgggacg ccctcgagcc cgtgacagca gcagttgtca gctgggcagt 60 ttcagtgccc cccacccccg acagaggacg ttcgtcacta tctgtggaaa catttgtggt 120 tgtcactctg gagaaggggt gctcctggca tctggtatat aggggccagg ggctggtaaa 180 cgtcctacaa ccacaggacc accccctgca aagactcctg tagtccctgg ggccatggcg 240 ctagctggga aacagatact ggagagggag gagtcctcgg ggctgttggt gactttatcc 300 cctcttcatg tgctggaggg tatggggggg tcttccagga agcctgggag gagaagtgga 360 gacagaggct ggaccactgc atttcactgg aacgtttgcc tgaggagaag tgggctcagg 420 agaggttttc tgttttatga aagaagcaat cactgcgttg ttgatgggga acatcttgat 480 ggagaaatcc aagggcaagg gatagcagcc aggctctgcc cctgagtgaa cctaatgggc 540 ttgagggtgt ccacgtggca ctgtcccttg acagggtcag ggcagctgct agggtgggac 600 gagcagttgg ggtct 615 // ID JK123790; SV 1; linear; mRNA; EST; MAM; 387 BP. XX AC JK123790; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD06FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-387 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 779d34043bb8df06feace840d9f20916. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2598 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000058403 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000002340 CC High quality match with Homo sapiens transcript: ENST00000465618 CC (RPL3-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000014001 (XM_001095608.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000015830 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000019414 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000066174 (XR_024073.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000013745 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01AGTOY, F01W12W02HFU7S, F0DTF6N02FV4I0, F0Z8TLB01AHMKE, CC F1C5L3D02DUN0S CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874255|FR874255.1:100040399-100040001. XX FH Key Location/Qualifiers FH FT source 1..387 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 387 BP; 106 A; 84 C; 108 G; 89 T; 0 other; aggatggcaa gctgatcaag aacaatgctt ccactgacta tgacctgtct gataagagca 60 tcaatcctct gggtgggttt gtccactatg gtgaagtgac caatgacttt gtcatgctga 120 aaggctgtgt ggtgggaacc aagaagcggg tgctcaccct ccgcaagtcc ttgctggtgc 180 agacgaagcg gcgggctctg gagaagattg accttaagtt cattgacacc acctccaagt 240 ttggccatgg ccgcttccag accatggaag agaagaaagc attcatggga ccactcaaga 300 aagatcgaat tgcaaaggaa gaaggagctt aatgccggga gcagattttg cagctggtgg 360 ggtctcaata aaagttattt tccactg 387 // ID JK123791; SV 1; linear; mRNA; EST; MAM; 416 BP. XX AC JK123791; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD07FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-416 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; ade57bef33054e5f18d89f87e93beb6c. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig530 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000008111 CC High quality match with Homo sapiens transcript: ENST00000400448 CC (HNRNPA1P7-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000018222 (LOC710473) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000022532 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000012717 (XR_021463.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BWDJC02GJTWA, FZ8FYPF01DV2Y3, FZ8FYPF01ERIPA, FZ8FYPF01BFHM4, CC FZ8FYPF01D3E32 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874249|FR874249.1:143329347-143329194. XX FH Key Location/Qualifiers FH FT source 1..416 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 416 BP; 127 A; 64 C; 87 G; 138 T; 0 other; ggccgcacaa ctttgtacaa gaaagttggg tttttttttt aatgtgctgt gtaaagttag 60 tctactctga agccatcttg gtaaacttcc ccaacagtgt gaagttagaa ttccttcagg 120 gtgatgccag gttctatttg gaatttatat acaacctgct tgggtggaga agccattgtc 180 ttcggaaacc ttggtgtagt tgaactgata gttactgttg tgacctgaag ttcaccgtta 240 aaagggatta cccaagcaaa atcatggaat tattggttat aaaagtgatt gttggcacat 300 cctatgcaat atatctaaat tgaataatgg taccagataa aattatagat gggaatgaag 360 cttgtgtatc atccattatc atgtgtaatc aataaacgat ttaattctct tgaaaa 416 // ID JK123792; SV 1; linear; mRNA; EST; MAM; 608 BP. XX AC JK123792; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD08FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-608 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 7277e32550fd733bc7ea72bdeb1c0aea. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H02FKXX4, F0BOF8H01DK6GL, F0DTF6N02GE51M, F0BOF8H01DC9WN, CC F0DTF6N02FO1TR CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:170719050-170718985. XX FH Key Location/Qualifiers FH FT source 1..608 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 608 BP; 109 A; 204 C; 169 G; 126 T; 0 other; tggacctcct gtgcaagaac atgaagcacc tgtggttctt ccttctcctg gtggcagctc 60 ccagatgggt cctgtcccag gtgcagctgc aggagtcggg cccagggctg gtgaaacctt 120 cggagaccct gtccctcacc tgcgctgtct ctggtggctc catcagcggt gattatggct 180 ggggctggat ccgccagccc ccagggaagg ggctggagtg gattgggatc atctatacta 240 gtagtgggaa cacctactac aacccctccc tcaagagtcg agtctccatt tcaaaagacg 300 cgtccaacaa ccagttctcc ctgaggctga gctctctgac cgccgcggac acggccgtgt 360 attactgtgc gagatccccc gatgagactg agtacaagtg gtacttggat ctctggggcc 420 gtggcaccct catcaccgtc tcctcagcct ccaccaaggg cccatcggtc ttccccctgg 480 cgccctcctc caggagcacc tccgagagca cagcggccct gggctgcctg gtcaaggact 540 acttccccga acccgtgact gtgtcgtgga actcaggctc cctgaccagc ggcgtgcaca 600 ccttcccg 608 // ID JK123793; SV 1; linear; mRNA; EST; MAM; 597 BP. XX AC JK123793; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD09FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-597 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 36d59aca02b10b812e37bd4b31bbfd65. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2377 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000006778 CC High quality match with Homo sapiens transcript: ENST00000444961 CC (IFI27-202) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000033466 (NP_001041634.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000012290 (XM_001152284.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z1IVZ01DQSPJ, F0Z1IVZ01CP4WJ, F0X19WM01D5WR6, F0M2C4T02FGSL5, CC F01W12W01DPJDP CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874252|FR874252.1:67063035-67063626. XX FH Key Location/Qualifiers FH FT source 1..597 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 597 BP; 127 A; 168 C; 171 G; 131 T; 0 other; gtcagcttca cattctcagg aactctcctt ctctgggcca cggaattaac ccgagcagcc 60 atggtggcct cagctttcac ctcatcagca gtgaccggtg tggccaaagt ggccagggtg 120 gcctctagct gtgccgtagt cttccccctg gcaaggattg ctacagtcgt gattggagga 180 gttgtggccg tggaggctgt gcccatggtg ctcagtgtca tgggtttcac tgcgacggga 240 atcacctcgt cctccatagc agccaagatg atgtccgcag cggccattgc caatgggggt 300 ggagttgccc cgggcagcct tgtggctatt ctgcagtcac tgggagcaac gggactctcc 360 gggttgacca gattcatcct gggctccact gggtctgcca ttgcggctgg cattgcgagg 420 ttctactagc tccctgcccc tcgccctgca gagaagagaa ccgtgccagg ggagaaggca 480 cccagccatc ctgacccagc gaagagccaa ctatcccaaa tatacctggg gtgaaatata 540 ccagattctg cacgcgcaga ggaaaataag aaataaagat gaattgttgc aactctt 597 // ID JK123794; SV 1; linear; mRNA; EST; MAM; 688 BP. XX AC JK123794; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD11FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-688 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; a155aa2330977ea37a61d3234f203262. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3644 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000014446 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000022907 CC High quality match with Homo sapiens transcript: ENST00000397128 CC (AC008810.1-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000033499 (XM_001085949.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000031224 CC High quality match with Pongo abelii transcript: ENSPPYT00000017927 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1HESOY02HVNRE, F0Z8TLB02H9JBF, F0Z8TLB01DOLBE, F0BWDJC02JQXAZ, CC F01W12W01D7LQZ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874249|FR874249.1:40822924-40822822. XX FH Key Location/Qualifiers FH FT source 1..688 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 688 BP; 207 A; 125 C; 173 G; 183 T; 0 other; aagatggcga cagccgagaa gcagaaacac gacgggcggg tgaagatcgg ccactacatc 60 ctgggtgaca cgctgggggt cggcaccttc ggcaaagtga aggttggcaa acatgaattg 120 actgggcata aagttgctgt gaagatactc aatcgacaga agattcggag ccttgatgtg 180 gtaggaaaaa tccgcagaga aattcagaac ctcaagcttt tcaggcatcc tcatataatt 240 aaactgtacc aggtcatcag tacaccatct gatattttca tggtgatgga atatgtctca 300 ggaggagagc tatttgatta tatctgtaag aatggaaggc tggatgaaaa agaaagtcga 360 cgtctgttcc aacagatcct ttctggtgtg gattattgtc acaggcatat ggtagtccat 420 agagatttga aacctgaaaa tgtcctgctt gatgcacaca tgaatgcaaa gatagccgat 480 tttggtcttt caaacatgat gtcagatggt gaatttttaa gaacaagttg tggctcaccc 540 aactatgctg caccagaagt aatttcagga agattgtatg caggcccaga ggtagatata 600 tggagcagtg gggttattct ctatgcttta ttatgtggaa cccttccatt tgatgatgac 660 catgtgccaa ctctttttaa gaagatat 688 // ID JK123795; SV 1; linear; mRNA; EST; MAM; 691 BP. XX AC JK123795; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD12FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-691 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 82b895cc981c4bd07b36d6bd1ef1d309. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H02F6VF3, F0BOF8H01BBUDP, FZ8FYPF02FVZ1D, F1HESOY01DXUNK, CC F0M2C4T02HJNXW CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:220793351-220793037. XX FH Key Location/Qualifiers FH FT source 1..691 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 691 BP; 209 A; 139 C; 167 G; 176 T; 0 other; cggaccagtc gaagaatcct gcgtcagaat cgagagaacg aacaagcctt catcgccatg 60 gcacatttat atagaggaaa aggagggaga acgttgcggg aagtcaggga ccttgaacgc 120 agggactggc tgaagccacg gcagaaaaac ataaaatgtg aagatttcac ggacatttat 180 tagttctcca aagttaatac ttttataatt tcttatgtct gtctttactt taatctctta 240 aattccatca tcttctttgt aaactgagga gggtgtatgt cacttcagga ccctgtgatg 300 attgccttaa actgcacaaa ttgtttgtgg agcatgtgtg tttgaacaat atgaaatctg 360 ggcatctcaa agaaagaata agataacagc aatgttcagg gaacaagaga ggcaaccttg 420 aactggctgc cggtgagcca gacggaacag agccatattt cttctcttat gcaaatagga 480 gaaatatcgc tgaattcttt ttctcagcaa ggaacatccc tgagaaagag aatgcactct 540 gagggtaggc ctataaacga cccccttgga ggagtgccgc cttttacggt tgaagccgaa 600 gggatgaaat aagccccggc ctcctgtagc gctcccaggc ttattaggac gaggaaattc 660 ctacctaata aattttgggc aggcaggttg t 691 // ID JK123796; SV 1; linear; mRNA; EST; MAM; 502 BP. XX AC JK123796; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD13FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-502 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; b0584e4ad47ac22444f370d097bb5eb3. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1888 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000003053 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000029072 CC High quality match with Homo sapiens transcript: ENST00000549224 CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000041189 (NP_001041711.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000006267 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000063920 (NM_001009058.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000019065 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X19WM01AHSL2, F1RTSFR02HDVX0, F0BWDJC01D7TXJ, FZ8A1BE01BVBGR, CC F0BOF8H01BVTK7 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:31172091-31172035. XX FH Key Location/Qualifiers FH FT source 1..502 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 502 BP; 97 A; 128 C; 139 G; 138 T; 0 other; gtcatgtgca gcatgagggt ctgcccgagc ccctcaccct gagatgggag ccgtcttccc 60 aatccaccat ccccatcgtg ggcgtcattg ctggcctggt tctgcttgga gctgtggtca 120 ctggagctgt ggttgctgct gtgatgtgga ggaggaagag ctcagataga aaagcaggga 180 gctactctca ggctgcaagc agtgacagtg cccaaggttc tgatgtgtct cttacggctt 240 gtaaagtgtg agacagctaa cttgtgtggg actgcgaggc aagatttgtt catgccttct 300 ctttgtgact tgaagaaccc tgacgttctt tctgcaaagg cacctgaata tgtctgtgtt 360 cctgtaggca taatgtgagg aggtggggag aacaccccac ctccgtgtct accatgaccc 420 tcttccccac actgatctgt gttccctccc catttatctt tcctgttcca gaaaggtggg 480 gctgggatgt ctctatctct at 502 // ID JK123797; SV 1; linear; mRNA; EST; MAM; 675 BP. XX AC JK123797; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD14FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-675 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 0d6dde929703e9307bf418b8aa5acfc3. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000028117 CC High quality match with Homo sapiens transcript: ENST00000548125 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000008566 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000009902 (XM_509338.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0OTZ2I02JQK0J, F0Q2XTE01AUGQR, F0X5I7101E0881, F1C5L3D03GKPOP CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:108631689-108632360. XX FH Key Location/Qualifiers FH FT source 1..675 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 675 BP; 202 A; 99 C; 127 G; 247 T; 0 other; tgcttcattg ctagtttgta tttctaactt ctacagttgt agactccact gtgctttgta 60 tctgaatttc tcagtataga cattttgttt agtgtatgct tgcatattta ttttcaactt 120 tgcctgtctt taaaattgct tgaggaaaaa tggttgtaat taatttctgc tacagaaaag 180 ccacccggta cgttttgtct catcaagatt gttttaaatt ctaaactata actttgttca 240 gaggggcttt tgcaatgata gcagaaaact gtacaaatgt acagttagtt atagaggttc 300 ttgttgaaat gaacttacca tttgatgata tgtatgtaca gctgtatact tcagtctctt 360 ttagtttact tagaaagact agcaatttga cctgttaaac aggactagtt cacatcaaga 420 aattcaggtt agtgtataca cctggaggca tctgttattc agcttatcct ttgagtgggt 480 atttggcaca atgaggatta acttatgtga cccacttgaa tggctgatct aatatgttga 540 cattatgcat tctgtactta gtgaaatgtc agacgaaaat aactgatgaa taattttttt 600 gtattaaagg gatgggaaaa gaacacatga atttgttaat aaagcactat gatctgcaaa 660 agatggaatg tttca 675 // ID JK123798; SV 1; linear; mRNA; EST; MAM; 643 BP. XX AC JK123798; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD15FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-643 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; e2640ad2db1f66a144cd2385091152af. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3357 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000023230 CC High quality match with Homo sapiens transcript: ENST00000494664 CC (C3orf1-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000016830 (XM_001109560.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000006304 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000028499 (XM_516667.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1BDRJG03HIEAO, F1C5L3D03FSH8P, F1GITI002IQUT8, F1C5L3D03G11J6, CC F0X19WM02JEAB7 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874245|FR874245.1:39625724-39626180. XX FH Key Location/Qualifiers FH FT source 1..643 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 643 BP; 198 A; 125 C; 142 G; 178 T; 0 other; ttcacacctg tcacgggaag tctttttaga ataaacttag gcctgcgtgg cctggtggct 60 ggtggtataa ttggagcctt gctgggtact cctgtaggag gcctgctgat ggcatttcag 120 aagtactctg gtgagactat tcaggagaga aaacagaaga atcgaaaggc actccatgag 180 ctaaaactga aagagtggaa agccagacta caacttactg aggacctcac tgagaaaatt 240 gaaagtagtt tacagaaaaa tgaacctgag agtgatgcta agaaaattga agcactgcta 300 aaccttccta gaaacccttc agtaatagat aaacaagaca aagactgaaa gtgctctgaa 360 cttgaaactc actggagagt tgaagggagc tatgctgcca tgtccgttga atgccaacag 420 acaggccact ctgtggtcag cctgctgaca aatgtaagtg ctggtacctg tggtggcagt 480 ggcttgctct tgtccttttc tttttaagaa tggagctgtt gtactctgtc tttacttttc 540 cttaaattta aatacatatt tatgtttgta ttaatctatc aagatataca tacatgacta 600 tatccaccca cctagatttt aagcagtaaa taaaacattt ccc 643 // ID JK123799; SV 1; linear; mRNA; EST; MAM; 665 BP. XX AC JK123799; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD16FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-665 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 4af17df58a375dee369395141cdce2c7. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig635 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000030076 (LOC100401038) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000006549 CC High quality match with Homo sapiens transcript: ENST00000531188 CC (RPS3-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000043841 (RPS3) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000023313 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000029233 (XR_024900.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000004387 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000008637 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB01C7IZU, F0SMK5U02IZ0ZO, F1HESOY01BW47R, F0DTF6N01B005G, CC F0DTF6N01AY32I CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874258|FR874258.1:97164035-97164691. XX FH Key Location/Qualifiers FH FT source 1..665 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 665 BP; 160 A; 143 C; 209 G; 153 T; 0 other; gcggcggcaa gatggccgtg caaatttcca agaagaggaa gtttgtcgct gatggcatct 60 tcaaagctga actgaatgag tttcttactc gggagctggc tgaagatggc tactctggag 120 ttgaggtgcg agttacacca actaggacag aaatcattat tttagccacc agaacacaga 180 atgttcttgg tgagaagggc cggcggattc gggaactgac tgctgtagtt cagaagaggt 240 ttggctttcc agagggcagt gtagagcttt atgctgaaaa ggtggccact agaggtctgt 300 gtgccattgc ccaggcagag tctctgcgtt acaaactcct aggagggctt gctgtgcgga 360 gggcctgcta tggtgtgctg cggttcatca tggagagcgg agccaaaggc tgcgaggtcg 420 tggtgtctgg gaaactccga ggacagaggg ctaaatccat gaagtttgtg gatggcctga 480 tgatccacag cggagaccct gttaactact acgttgacac tgctgtgcgc catgtgttgc 540 tcagacaggg tgtgctgggc atcaaggtga agatcatgct gccctgggac ccaactggta 600 agattggccc taagaagccc ctgcctgacc acgtgagcat cgtggaaccc aaagatgaaa 660 tactg 665 // ID JK123800; SV 1; linear; mRNA; EST; MAM; 658 BP. XX AC JK123800; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD17FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-658 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; fed5baf8ae163538f08903a770379e11. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0DTF6N02IIS6L, F0DRJ6O01CWY6X, FZ8FYPF01B8XME, F1C5L3D03GUHL2, CC F1HESOY02II5ZM CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:49272045-49271815. XX FH Key Location/Qualifiers FH FT source 1..658 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 658 BP; 146 A; 196 C; 168 G; 148 T; 0 other; ccccacgaga gcattcaacc aagtacaggg tcctgtggga cacacccatg aagtgggccc 60 tatcttggcc atattctccc accctccact gcagtgaggc tgaggttctg gaccacagca 120 agaacccttg tgaagactct ttcctgccag acacagaggg ccacacctac gtggccttta 180 ttcgaatgga gaaagatgat gacttcacca cctggaccca gcttgccaag tgcctccata 240 tctgggacct ggatgtgcgt ggcaaccacc ggggcctatg gcgattgttt cggaagaaga 300 accacttcct ggtggtgggg gtcccggctt ccccctactc gtgagtgacc cttttctgcc 360 ctgggaaacg ggagatccag ggacatagct aatggcttct tccctataca tccttgtatt 420 attttcccag agtgaagaag ccaccctcag tcaccccaat tttcctggag ccacccccaa 480 aagaggaggg agccccagga gccgcagaac agacatgaga cctcctccag gaccctgcgg 540 ggctgggtac tgtgtacccc caggcgggct agcccttccc tccatcctgt aggattttgt 600 agatgctggt aggggctggg gctaccttgt ttttaacatg agacttaatt actaactc 658 // ID JK123801; SV 1; linear; mRNA; EST; MAM; 562 BP. XX AC JK123801; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD18FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-562 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; d69399e795e2f975bb7876a09a922364. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Homo sapiens transcript: ENST00000453744 CC (UBE2D3-206) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX02ICSAU, F1IUBWX01B8DNI, F0DTF6N01CRPML, F0DTF6N01EERCS, CC F0BWDJC01A3MSO CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874248|FR874248.1:96422597-96422030. XX FH Key Location/Qualifiers FH FT source 1..562 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 562 BP; 153 A; 71 C; 118 G; 220 T; 0 other; tttactagtg ccatgtatag gacccaactg agtattagaa tcatttttga ctatgtgtgt 60 gtatttccta gatctttcaa tgcgtaaact cttaagaatt aaggtccagt tggcctgtta 120 atggtaaatt tacattttaa gtgactcaat ttgtttttcc tgggcaagtt tgcaatgtga 180 taatcagatt ttttaaagct gattaatttg ctttcttgtg tgggtgtact cacattttaa 240 agtatgaacc acagttaact agtggtctca ggggtagtga aacactcact tttttttgtt 300 ttgttttttt tggtattttt ttgaaatggc ttagttgaag tatacttaaa gggactgatc 360 atgctgtgtt agtgatttgg gtgggggggg gttaactcaa attagccatg ttttgtgttg 420 gcataacaaa actgttaatg attgttgatt acacttttaa gtgaatttgt cttttatgag 480 gaacccagtg caagtcacta aatattgtct aatagtgaca tgtgcataag acttgtaata 540 gctaaagtta attgaagctt aa 562 // ID JK123802; SV 1; linear; mRNA; EST; MAM; 610 BP. XX AC JK123802; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD19FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-610 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; df10544b0a2faa0b2e85e7260655d45f. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2101 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000039703 CC High quality match with Homo sapiens transcript: ENST00000335295 CC (HBB-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000006876 (HBB_MACMU) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000006177 (HBB_PANTR) CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE02JXKN0, FZ8A1BE02IQM7U, F0M2C4T01BCZEQ, F1IUBWX01EIKD3, CC F1HESOY02FXE10 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:68763681-68763942. XX FH Key Location/Qualifiers FH FT source 1..610 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 610 BP; 134 A; 151 C; 166 G; 159 T; 0 other; gacacaactg tgttcactag caacctcaga caccatggtg catctgactc ctgaggagaa 60 gactgccgtt accaccctgt ggggcaaggt gaacgtggat gaagttggtg gtgaggccct 120 gggcaggctg ctggtggtct acccttggac ccagaggttc tttgagtcct ttggggatct 180 gtcctctcct gatgctgtta tgggcaaccc taaggtgaag gctcatggca agaaagtgct 240 tggtgccttt agtgatggcc tggctcacct ggacaacctc aagggcacct ttgcccagct 300 gagtgagctg cactgtgaca agctgcatgt ggatcctgag aacttcaagc tcctgggcaa 360 cgtgctggtg tgtgtgctgg cccatcactt tggcaaagaa ttcaccccgc aagtgcaggc 420 tgcctatcag aaagtggtgg ctggtgtggc taatgccctg gcccacaagt accactaagt 480 tcactttctt gctgtccaat ttctaccaaa ggttcctttg ttcccaaagt ccaactactg 540 aactggggga tattatgaag ggccttgagg atctggattc tgcctaataa aaaacattta 600 ttttcattgc 610 // ID JK123803; SV 1; linear; mRNA; EST; MAM; 727 BP. XX AC JK123803; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD20FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-727 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 234691ef707b12725c298dc10a4add28. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3645 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000024071 CC High quality match with Homo sapiens transcript: ENST00000263384 CC (FAM32A-201) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000006920 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000019632 (XM_512465.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000011279 CC (FA32A_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX01D16DE, F0ZWYBK01DCXV5, F0BWDJC01ESKAD, FZOOKC302F66U7, CC F0BWDJC01EV5WG CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874253|FR874253.1:62018751-62019172. XX FH Key Location/Qualifiers FH FT source 1..727 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 727 BP; 213 A; 175 C; 190 G; 149 T; 0 other; tacatctgcc gaaccaccgg agagtgttat ggaggcctac gagcaggtcc aaaagggacc 60 cctgaagctg aaaggcgtcg cagagctggg agtgaccaag cggaagaaga aaaagaagga 120 caaagacaaa gcgaaactcc tggaagcaat gggaacgagc aaaaagaacg aggaggagaa 180 gcggcgcggc ctggacaagc ggaccccggc ccaggcggcc ttcgagaaaa tgcaggagaa 240 gcggcaaatg gaaaggatcc taaagaaggc atccaaaacc cacaaacaga gagtggagga 300 cttcaaccga cacctggaca cactcacgga gcattatgac attcccaaag tcagctggac 360 gaagtagcca cctgccccgg tatggagcag catcgaggat tcacaaaagg ccaccctggg 420 gttgtgtgtg tttcctttgg tatattccag aaacatggct ttacacacac ccttgcatct 480 tctgctacag actgcttttc gaagctgtgt accctcattc tggaacttga ttaaagtaag 540 attgtccttg tactccgttt aggcttcttg gcaacgtaca gaagataaca cccttttcgt 600 ttggatggaa agtttctaag tttatccaga ggtaaagccc attgtgtgtc cgtgccatgt 660 aaaaatgttt tcactcctga gttgcatcta acactctgag gccagccagc tgtcttctcc 720 tggatga 727 // ID JK123804; SV 1; linear; mRNA; EST; MAM; 592 BP. XX AC JK123804; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD21FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-592 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; e2ccebe397f0a5c129e1a6fc56b2f9bb. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2351 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000053637 (ITFG2) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000010523 CC High quality match with Homo sapiens transcript: ENST00000542548 CC (ITFG2-202) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000000097 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000008390 (XM_001154006.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000004919 CC (ITFG2_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0SMK5U02FK9R5, F1C5L3D02DQ0BA, F0SMK5U02I33WZ, FZ8A1BE01ED73Y, CC F0OTZ2I02FI8Y0 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874249|FR874249.1:107921742-107921173. XX FH Key Location/Qualifiers FH FT source 1..592 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 592 BP; 133 A; 151 C; 185 G; 123 T; 0 other; gttgtgagct ggtggtgggt tacacagacc gtgtggtgcg agctttccgc tgggaggagc 60 taggtgaggg tcccgaacat ctgacagggc agctggtgtc cctcaagaaa tggatgctgg 120 agggccaggt ggacagcctc tcagtgactc tgggaccact gggtgttcct gaactcatgg 180 tgtctcagcc aggctgtgct tatgcaattc tactgtgtac ctggaaaaag gacactgggt 240 cccctcctgc ctccgaaggg cccacggatg gcagtaggga gaccccagct gcccgagatg 300 tggtgctgca ccagacgtct ggccgtatcc acaacaagaa cgtctccact cacctaattg 360 gcaacatcaa acaaggccac ggcactgaga gtagtggctc tggcctcttt gccctgtgca 420 ccctggatgg gacactgaag ctcatggaag aaatggaaga agcagacaag ctgctgtggt 480 cagtgcaggt ggatcaccag ctctttgccc tggagaaact ggatgtcacc ggcaatgggc 540 atgaggaggt agttgcatgc gcctgggatg gacagacata catcatttga cc 592 // ID JK123805; SV 1; linear; mRNA; EST; MAM; 686 BP. XX AC JK123805; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD22FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-686 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 4286ab142de1c2d10f5455ea8875aba9. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000018812 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000016818 CC High quality match with Homo sapiens transcript: ENST00000343815 CC (UBAP2L-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000045894 (XM_001113770.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000010405 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000014388 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000002528 CC High quality match with Pongo abelii transcript: ENSPPYT00000000946 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB01CA21K, F01W12W02FQ6ZI, F0Z1IVZ02HPFQI, F0Z1IVZ02ISZFQ, CC F0DTF6N02I7SWN CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:133530471-133530581. XX FH Key Location/Qualifiers FH FT source 1..686 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 686 BP; 149 A; 236 C; 152 G; 149 T; 0 other; cctcccccag tggtcagtgt ctcctccagt ctcaatagtg gcagtagcct gggcctcagc 60 ctaggcagca actccactgt cacagcctcg actcgaagct cagttgctac gacttcagga 120 aaagctcctc ccaacctccc tcctggggtc ccgccgttgt tgcctaatcc gtatattatg 180 gctccagggc tgttacatgc ctacccgcca caagtatatg gttatgatga cttgcagatg 240 cttcagacaa gatttccatt ggattactac agcatcccat ttcccacacc cactactccg 300 ctgactggga gggatggtag cctggccagc aacccttatt ctggtgacct cacaaagttc 360 ggccgtgggg atgcctcctc cccagccccg gccacaacct tggcccaacc ccaacagaac 420 cagacgcaga cgcaccatac cacgcagcag acattcctga acccggcgct gcctcctggc 480 tacagttaca ccagcctgcc atactataca ggggtcccgg gcctccccag caccttccag 540 tatgggcctg ctgtgttccc ctgtggctcc tacctcttcc aagcagcatg gtgtgaatgt 600 cagtgtgaat gcatcggcca cccctttcca acagccgagt ggatatgggt ctcatggata 660 caacactgga agaaaatatc cacccc 686 // ID JK123806; SV 1; linear; mRNA; EST; MAM; 663 BP. XX AC JK123806; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD23FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-663 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f3156507e971be30851f5daa1d5d6f37. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1127 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000040479 (LOC100412650) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000002668 CC High quality match with Homo sapiens transcript: ENST00000380394 CC (RPS6-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000018897 (LOC712274) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000013278 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000022360 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000015131 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000030188 (XR_022222.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000022374 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Q2XTE01BVO1K, FZOOKC302HISCF, F01W12W02JYVPR, F0BOF8H01AFHNQ, CC F1GITI001ASW0D CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874245|FR874245.1:95419827-95419181. XX FH Key Location/Qualifiers FH FT source 1..663 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 663 BP; 198 A; 147 C; 173 G; 145 T; 0 other; ggcgttcagc tgcttcaaga tgaagctgaa catctccttc ccagccactg gctgccagaa 60 actcattgaa gtggacgatg aacgcaaact tcgtactttt tatgagaagc gtatggccac 120 agaagttgct gctgacgctc tgggtgaaga atggaagggt tatgtggtcc gaatcagtgg 180 tgggaacgac aaacaaggtt tccccatgaa gcagggtgtc ttgacccatg gccgtgtccg 240 cctgctactg agtaaggggc attcctgtta cagaccaagg agaacgggag aaagaaagag 300 aaaatcagtt cgtggttgca ttgtggatgc caatctgagc gttctcaact tggttattgt 360 aaaaaaagga gagaaggata ttcctggact gactgatact acagtgcctc gccgcctggg 420 ccccaaaaga gctagcagaa tccgcaaact tttcaatctc tctaaagaag atgacgtccg 480 ccagtatgtt gtaagaaagc ccttaaacaa agaaggtaag aaacctagga ccaaagcacc 540 caagattcag cgtcttgtta ctccacgtgt cctgcagcac aaacggcggc gtattgctct 600 gaagaagcag cgtactaaga aaaacaagga agaggctgca gaatatggct aaacttttgg 660 cca 663 // ID JK123807; SV 1; linear; mRNA; EST; MAM; 721 BP. XX AC JK123807; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YD24FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-721 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; a074a31d954213c131f56af852c57b8a. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3384 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000003070 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000025114 CC High quality match with Homo sapiens transcript: ENST00000457246 CC (CR753846.5-005) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000040913 (NP_001127767.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000004029 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000008553 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000063859 (O46851_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000020054 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZOOKC302IE0BK, F1IUBWX01DN8UD, F0Q2XTE01CSU45, F0OTZ2I02JUBQZ, CC F1RHTUQ02IH4VH CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:32585734-32585799. XX FH Key Location/Qualifiers FH FT source 1..721 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 721 BP; 158 A; 196 C; 184 G; 183 T; 0 other; actcagaaca gcaactgagc aactgctgag gctgccttgg gaagaggatg atcctaaaca 60 aagctctgat gttgggggcc ctcgctctga ccgctgtgat gagcccctgc ggaggtgaag 120 acattgtggc tgaccatgtc gcctcttacg gtgtaaacgt gtaccagtct tacggtccct 180 ctggccagta ctcccatgaa tttgatggag acgagcagtt ctacgtggac ctggggagga 240 aggagactgt ctggcggttg cctattccct accgatatgg ttttgacccg caatttgcat 300 tgagaaacat cgctgtgcta acacataact tgaacatcct gattaaacgc tccaactcta 360 ccgctgctac caatgaggtt cccgaggtca cagtgttttc caagtctccc gtgacgctgg 420 gtcaccccaa caccctcatc tgtcttgtgg acaacatctt tcctcctgtg gtcaacatca 480 catggttgag caatgggcac tcagtcacag aaggtgtttc tgagaccagc ttcctctcca 540 agagtgatca ttccttcttc aagatcagtt acctcacctt cctcccttct gctgatgaga 600 tttatgactg cagggtggag cactggggcc tggatgagcc tcttctgaaa cactgggagc 660 cggagattcc agcgtccatg tcagagctca cagagactgt ggtctgcgcc ctggggttgt 720 c 721 // ID JK123808; SV 1; linear; mRNA; EST; MAM; 652 BP. XX AC JK123808; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE01FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-652 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 137e264e73cb36500c40045268351117. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F01W12W02GSAVD, F01W12W02JEXT7, F1IUBWX02HLAKF, FZ8FYPF02GFF5D, CC F0BOF8H02HVQDL CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874256|FR874256.1:89829410-89829107. XX FH Key Location/Qualifiers FH FT source 1..652 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 652 BP; 182 A; 145 C; 180 G; 145 T; 0 other; gattttggtg tcactgtatc atttgtgcaa gttttgtgat attttggtta aataaacctg 60 gtgacccaga agtaaacagc gggacaccag aaaatcaact taaaaagctg agcaaataga 120 aaaatccttg ggtttgggag gagaatagga ttcatggggg aaatgggaaa gaaatagcta 180 gatttttctc tgaacaagca gcctatctca tatgattggc tttgagggag gtttttgttg 240 agggaaaggg taagatccct cactgtggct cactttcggc ggagggacca aggtggagat 300 caaacgagct gtggctgcac catctgtctt catcttcccg ccatctgagg atcaggtgaa 360 atctggaact gtctctgttg tgtgcctgct gaataacttc tatcccagag aggccagcgt 420 aaagtggaag gtggatggtg ccgtccaaac gggtaactcc caggagagtg tcacagagca 480 ggacagcaag gacaacacct acagcctgag cagcaccctg acgctgagca gcacagacta 540 ccagagtcac aaggtctatg cctgcgaagt cacccatcag ggcctgagct cgcccctcac 600 caagagcttc aacagagaag agtgttagag ggagaagtgc cccgggcccc cc 652 // ID JK123809; SV 1; linear; mRNA; EST; MAM; 541 BP. XX AC JK123809; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE02FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-541 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2b6383fbad3f7d4eac06b99c464dbeb5. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1385 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000028370 (Q58YJ5_9PRIM) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X19WM01A5WMM, F0OTZ2I02H6WVL, F1IUBWX01D6U23, FZ8A1BE01DHXQ0, CC F0Q2XTE01DGZJC CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874251|FR874251.1:108785075-108785530. XX FH Key Location/Qualifiers FH FT source 1..541 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 541 BP; 139 A; 73 C; 149 G; 160 T; 20 other; gccgcacaac tttgtacaag aaagttgggn nnnnnnnnnn nnnnnnnnnc atttgtatag 60 tggaaaggga gtggggtatt tatagggtaa acacaggtcc tatttcaaag atttttaatg 120 ggattagttc tgcgacgata ggtatgaagc tgtggttagc cccacagatt tctgagcatt 180 gtccataata gacgcctggt cgtgtggcgg taaatgtggt ttgatttaag cgtccgggta 240 ctgcatctgt ttttaaacct agtgtgggaa tagttcatga gtgtaggaca tcttgagatg 300 taattattat acggacgggg gcttcaattg ggagaaccac tcggttgtca acttctagaa 360 gtcgaaggtc tcctgggttt aagaataatg gaggcagtat gtaagaatta aagatgaggc 420 ctccgtagtc tgtgtattcg taggttcagt atcattgatg tccaattgat ttaatagtaa 480 aggaggggtt gttgatttcg tctgttaggt acagaatgcg tagagatggt agggcaatca 540 g 541 // ID JK123810; SV 1; linear; mRNA; EST; MAM; 686 BP. XX AC JK123810; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE03FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-686 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2d304d2f0ee7e470ca462937791bf865. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1844 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000015426 (LOC100405509) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000009222 CC High quality match with Homo sapiens transcript: ENST00000543155 CC (COPS7A-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000047302 (XM_001108483.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000013662 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000005841 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000005974 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000008485 (XM_001162932.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000004974 CC (CSN7A_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX01DOU3J, F0Z1IVZ01CYOT9, F0OTZ2I01D75XA, F0ZWYBK02FHXWE, CC F1C5L3D04JEC7T CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:6902576-6902620. XX FH Key Location/Qualifiers FH FT source 1..686 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 686 BP; 140 A; 190 C; 211 G; 145 T; 0 other; agcggcggcc ccgcccggtg cgctggaggt cgaagcttcc aggtagcggc ccgcagagcc 60 tgacccaggc tctggacatc ctgagcccag gtgcccccag ctcagtgcag tcatgagtgc 120 ggaagtgaag gtgacagggc agaaccagga gcaatttctg ctcctagcca agtcggccaa 180 gggggcagcg ctggccacac tcatccatca ggtgctggag gcccctggtg tctacgtatt 240 tggagaactg ctggacatgc ccaatgttag agagctggct gagagtgact ttgcctccac 300 cttccggctg ctcacagtgt ttgcttatgg gacatacgct gactacttag ctgaagcccg 360 gaatcttcct ccactaacag aggctcagaa gaataagctt cgacacctct cagttgtcac 420 cctggctgct aaagtaaagt gtatcccata tgcagtgttg ctggaggctc ttgccctgcg 480 taatgtgcgg cagctggaag accttgtgat tgaggctgtg tatgctgatg tgctccgtgg 540 ctccctggac cagcgcaacc agcggctcga ggttgactac agcatcgggc gggacatcca 600 gcgccaggac ctcaatgcca ttgcccgaac cctgcaggaa tggtgtgtgg gctgtgaggt 660 tgtgctgtca ggcattgaag gacagg 686 // ID JK123811; SV 1; linear; mRNA; EST; MAM; 728 BP. XX AC JK123811; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE04FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-728 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 101cb4296951ac4048c976ccb7f8bbd5. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3646 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000038555 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000029410 CC High quality match with Homo sapiens transcript: ENST00000544784 CC (ACTR3-204) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000021073 (XR_012216.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000014873 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000023004 (XR_021237.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000014863 CC (ARP3_PONAB) CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000014386 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BWDJC01AZ09X, F1GITI002G6SWI, F0X19WM02GZ6QI, F01W12W01EOXDS, CC F1HESOY01C1XVG CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874264|FR874264.1:67905816-67906544. XX FH Key Location/Qualifiers FH FT source 1..728 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 728 BP; 216 A; 135 C; 162 G; 215 T; 0 other; ggagcaagtg atctttaagt atttaagggc agaacctgaa gaccattatt ttcttttgac 60 tgaacctcca ttgaatactc cagaaaacag agaatatact gctgaaataa tgtttgagtc 120 tttcaatgtt ccaggcttgt acatcgccgt gcaggctgtt cttgccttag ctgcatcttg 180 gacctcaaga caagtaggag aacggacgtt gaccggtaca gtaatagaca gtggagatgg 240 tgtcactcat gtcattcctg tggctgaagg gtatgtgatt ggcagctgta ttaaacacat 300 tccaatcgca ggacgagata taacatattt tattcagcaa ctgctgagag accgagaagt 360 aggaatccct ccagaacaat ccttggaaac tgctaaggca gtaaaggagc gttatagtta 420 tgtctgccca gatttagtaa aagaatttaa caagtatgat acagatgggt caaaatggat 480 taaacagtat actggaatca atgctatctc aaagaaagaa ttttctatcg atgttggtta 540 tgagagattt ttgggacctg aaatcttttt tcatccagag tttgctaatc cagactttac 600 acagcctatc tcagaagttg tagatgaagt aattcagaat tgtcctattg atgtcaggcg 660 tcctctctac aagaatattg tcctctctgg aggtttcacc atgttcaggg actttggacg 720 tcgcttgc 728 // ID JK123812; SV 1; linear; mRNA; EST; MAM; 542 BP. XX AC JK123812; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE05FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-542 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1b57b3595b2c838016cf63f7d60638b2. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000003993 (LOC100399916) CC High quality match with Homo sapiens transcript: ENST00000398970 CC (CRK-002) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000015716 (XM_001154201.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RHTUQ01EL387, F01W12W01EI3EE, FZ8A1BE01BXFV3, F0DRJ6O02FQSXQ, CC F1IUBWX02H7HC7 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874259|FR874259.1:78947087-78946885. XX FH Key Location/Qualifiers FH FT source 1..542 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 542 BP; 131 A; 121 C; 153 G; 137 T; 0 other; aggtcactct tctacagcag ttaactagag tcctaactga tgggatgcag cagcgccatt 60 tatcctgaag gccagtatcc tattaggaga ctcttggaat tcttaggttc tactcagagt 120 ggaaggacca atcacctcta atattctgtg gaaggttttg gggccaaatt ctgccctctg 180 cattctgtgc aacttgtata aaagtcaagt tagtattaca tgagtttggg gtagggttag 240 tgctttgaaa aatgttgaac tggccgggcg gtggctcacg cctgtaatcc cagcactttg 300 ggaggccgag gtgggtggat cacgaggtca ggagttcgag accagcctgg ccaacgtggt 360 gaatccccat ctctactaaa gatacaaaaa attagctggg tgtggtggcg cacacctgta 420 atcccagcta ctcgggaggc tgaagcagga gaattgcttg aacctgggag ttggaggttg 480 ccgtgagccg agatagcacc actgccctcc agcctgggtg atagggcgag actctcttct 540 ca 542 // ID JK123813; SV 1; linear; mRNA; EST; MAM; 536 BP. XX AC JK123813; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE06FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-536 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 8f574e4fa13b466d4f4d2844d5226147. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig961 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000000126 (LOC100396646) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000002952 CC High quality match with Homo sapiens transcript: ENST00000270625 CC (RPS11-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000010558 (Q3YAQ2_MACMU) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000002540 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000024106 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000000370 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000021005 (XM_001172982.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BWDJC02FYJPQ, F0OTZ2I02JKJX7, FZ8A1BE01DQFX4, F0Q2XTE02JAU35, CC F0OTZ2I01DT568 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:129360610-129360077. XX FH Key Location/Qualifiers FH FT source 1..536 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 536 BP; 145 A; 149 C; 139 G; 103 T; 0 other; ggaagatggc ggacattcag acagagcgtg cctaccaaaa gcagccgacc atctttcaaa 60 acaagaagag ggtcctgctg ggagaaactg gcaaggagaa gctcccgcgg tactacaaga 120 acatcggtct gggcttcaag acacccaaag aggctattga gggcacttac atcgacaaga 180 aatgcccctt cactggtaat gtctccattc gagggcggat cctctctggt gtggtgacga 240 agatgaagat gcagaggacc attgtcatcc gccgagacta cctgcactac atccgcaagt 300 acaaccgctt cgagaagcgc cacaagaaca tgtctgtgca cctgtccccc tgcttcaggg 360 acgtccagat cggtgacatc gtcacagtgg gtgagtgtcg gcccctgagc aagacagtgc 420 gcttcaacgt gctcaaggtc accaaggctg ccggcaccaa gaagcagttc cagaagttct 480 gagtctggac atcggcccgc tccccacaat gaaataaaag ttattttctc attccc 536 // ID JK123814; SV 1; linear; mRNA; EST; MAM; 570 BP. XX AC JK123814; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE07FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-570 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; a57b8deca9b095f70b538cc4fb1752d8. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1376 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000039794 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000029102 CC High quality match with Homo sapiens transcript: ENST00000354532 CC (ARHGEF1-204) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000031383 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Q2XTE02HT8JS, F1IUBWX02FUDJY, F1RHTUQ02GS7MC, F1GITI002GUCVU, CC F0ZWYBK01BCYQ6 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/). XX FH Key Location/Qualifiers FH FT source 1..570 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 570 BP; 111 A; 197 C; 168 G; 94 T; 0 other; ccaagaagaa attagtccac gagggcccac tgacgtggcg ggtgacaaag gacaaggctg 60 tggaggtgca tgtgctgctg ctggatgacc tgctgctgct gctccagcgc caggatgagc 120 ggctgctgct caagtcccat agccggacgc tgacgcccac gcccgacggc aagaccatgc 180 tgcggcctgt gctgcggctc acctctgcca tgacccgcga ggtggccacc gatcacaaag 240 ccttctacgt cctttttacc tgggaccagg aggcccagat atacgagctg gtggcacaga 300 ctgtgtcgga gcggaagaac tggtgtgccc tcatcaccga gactgccgga tccctgaaag 360 tccctgcccc tgcctctcgc cctaagcccc ggcccagccc gagcagcacc cgagaacccc 420 tcctcagcag ctctgagaac ggcaatggtg gccgagagac gtctccggct gatgcccgga 480 ccgagagaat cctcagtgac ctcctgccct tctgcagacc aggccccgag ggccagctcg 540 ctgccacggc ccttcggaaa gtgctgtccc 570 // ID JK123815; SV 1; linear; mRNA; EST; MAM; 424 BP. XX AC JK123815; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE08FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-424 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 86a3673e0da5a627791bccd770f87c30. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig846 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000013096 (LOC100413255) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000004131 CC High quality match with Homo sapiens transcript: ENST00000348462 CC (RPL35-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000007404 (XP_001082318.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000002918 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000022068 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000008516 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000039513 (XR_023153.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000022874 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z1IVZ01BUP10, F0DRJ6O01D1HQT, FZ8A1BE01CRJ3A, F0Z8TLB01E2T9C, CC FZ8A1BE02FFXJ5 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874248|FR874248.1:138080222-138080611. XX FH Key Location/Qualifiers FH FT source 1..424 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 424 BP; 119 A; 116 C; 128 G; 61 T; 0 other; gcggccgcct gtgtagcaat ggccaagatc aaggctcgag atcttcgcgg gaagaagaag 60 gaggagctgc tgaaacagct ggacgacctg aaggtggaac tgtcccagct gcgcgtcgcc 120 aaagtgacgg gcggtgcggc ctccaagctg tctaagatac gagtcgtccg caaatccatc 180 gcccgtgttc tcacagtcat taaccagact cagaaagaaa acctcaggaa attctacaag 240 ggcaagaagt acaagcccct ggacctgcgg cctaagaaga cacgcgccat gcgccgccgg 300 ctcaacaaac acgaggagaa cctgaagacc aagaagcagc agcggaagga gcggctatac 360 ccgctgcgga agtacgcggt caaggcctga gtggcgcgtt gtcaataaag cacagctggc 420 tgag 424 // ID JK123816; SV 1; linear; mRNA; EST; MAM; 380 BP. XX AC JK123816; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE09FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-380 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; fb2706cdf5ff65b7024e5a06e91d4304. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3684 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000004891 CC High quality match with Homo sapiens transcript: ENST00000515766 CC (MAEA-011) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000011685 (XM_001092178.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000029543 (XM_001143429.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000016887 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE02IE50F, FZ8A1BE02HY62O, FZ8A1BE02JCPPK, FZ8A1BE02GI211, CC FZ8A1BE02GDBVO CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:2645891-2645513. XX FH Key Location/Qualifiers FH FT source 1..380 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 380 BP; 97 A; 81 C; 89 G; 113 T; 0 other; tgcctcgcgg gccgtgtccg cgggactgtg ttcgtacgtg cgtagtttcg ataccacatc 60 acggggctgt gttcctacgt gcgtagtttc gatatcacac cctccgtgtg ccgccttact 120 tcctgctttg agaatgtata acgtggaaat ccacaggacc aagtttctgc agaggccttg 180 ccggatggtt ccataactgt agagtccaaa tactatccat tacagaaatt aatcgttcag 240 ttgagagaag tactgatgac ttttcaaaac aaatgaacca tcgtagttga cagagaacca 300 tattgtagag gtttgtagtt agtgcttatt tttgcatgtt gatgttgact agctaataaa 360 ctgtaaatgt aaaccatgcg 380 // ID JK123817; SV 1; linear; mRNA; EST; MAM; 530 BP. XX AC JK123817; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE10FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-530 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 737432aac98d3636fd966f67a2eedca4. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0SMK5U02GYDVS, F0X5I7101D603P, F0M2C4T01EB70M, F1GITI002GW19H, CC F0BOF8H02IMYLU CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:41392632-41392595. XX FH Key Location/Qualifiers FH FT source 1..530 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 530 BP; 101 A; 78 C; 128 G; 62 T; 161 other; gggagcggag agcggacccc agagagccct gagcagccnn nnnnnnnnnn nnnnnnnnnn 60 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 120 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 180 nnnnnnnnnn nnnnnnnnna agcccggcac tacgggcagc ggcgcaggga gcggtggccc 240 gggcggcctc acatcggcgg cgcctgccgg cggggacaag aaggtcatcg caacgaaggt 300 tttgggaaca gtaaaatggt tcaatgtaag gaacggatat ggtttcatca acaggaatga 360 caccaaggaa gatgtgtttg tacaccagac tgccataaag aagaataacc ccaggaagta 420 ccttcgcagt gtaggagatg gagagactgt ggagtttgat gttgttgaag gagaaaaggg 480 tgcggaggca gcaaatgtta caggtcctgg tggtgttcca gttcaaggca 530 // ID JK123818; SV 1; linear; mRNA; EST; MAM; 607 BP. XX AC JK123818; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE11FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-607 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f03f4bdece23cc61dab9215de8975d6b. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RTSFR01A0S9R, F0Z8TLB01EUQMA, F0M2C4T02FX9UA, F0BWDJC02HXP8J, CC F0OTZ2I02IHG9U CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874259|FR874259.1:78657719-78657817. XX FH Key Location/Qualifiers FH FT source 1..607 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 607 BP; 166 A; 123 C; 150 G; 168 T; 0 other; aacagactca gaaaagttga gtaactggct caagtcacat agctagtagt agagctaagt 60 ttagattcct gtgtctctgt gattctataa tacatgtttt tctaccagcc tctttcacct 120 gcttactgta ggctgtggca gggaacttca tccttttgga gcctcagttt tcccatctct 180 aaaatgaggg gtattctgta tttcctcatg gggatgttat gaggatagtg aaaacaaatg 240 taaaagcaac atagtaagat cttaaaaata tgtgataggg ccgggcatgg tggctccttc 300 ctgtaattcc tagcactctg ggttactgag atgggcggat cactgtcagt agtttgagac 360 cagcctggcc aacatggcga attcctgtct ctgctacaaa tacaaaaatt agccgaatgt 420 ggtgatgcat gccagtaaat cccagctact tgggaggatg aggcatgaga attgcttgaa 480 ctgcactcca gcctgggtga cagggagact ctgtctcagg aaaaaaaaaa aggctgggca 540 tggtggctct cacctgtaat tctggcactt tagaaaggct gaggtagcag tatcactttg 600 agcccag 607 // ID JK123819; SV 1; linear; mRNA; EST; MAM; 592 BP. XX AC JK123819; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE12FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-592 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 13922764595a6a25d89a96a8d7a03e27. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2101 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000039703 CC High quality match with Homo sapiens transcript: ENST00000335295 CC (HBB-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000006876 (HBB_MACMU) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000022127 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000006177 (HBB_PANTR) CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE02JXKN0, FZ8A1BE02IQM7U, F0M2C4T01BCZEQ, F1IUBWX01EIKD3, CC F1HESOY02FXE10 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:68763681-68763922. XX FH Key Location/Qualifiers FH FT source 1..592 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 592 BP; 126 A; 149 C; 166 G; 151 T; 0 other; ctgacacaac tgtgttcact agcaacctca gacaccatgg tgcatctgac tcctgaggag 60 aagactgccg ttaccaccct gtggggcaag gtgaacgtgg atgaagttgg tggtgaggcc 120 ctgggcaggc tgctggtggt ctacccttgg acccagaggt tctttgagtc ctttggggat 180 ctgtcctctc ctgatgctgt tatgggcaac cctaaggtga aggctcatgg caagaaagtg 240 cttggtgcct ttagtgatgg cctggctcac ctggacaacc tcaagggcac ctttgcccag 300 ctgagtgagc tgcactgtga caagctgcat gtggatcctg agaacttcaa gctcctgggc 360 aacgtgctgg tgtgtgtgct ggcccatcac tttggcaaag aattcacccc gcaagtgcag 420 gctgcctatc agaaagtggt ggctggtgtg gctaatgccc tggcccacaa gtaccactaa 480 gttcactttc ttgctgtcca atttctacca aaggttcctt tgttcccaaa gtccaactac 540 tgaactgggg ggatattatg aagggccttg aggatctgga ttctgcctaa ta 592 // ID JK123820; SV 1; linear; mRNA; EST; MAM; 230 BP. XX AC JK123820; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE13FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-230 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 758e0c6551164a9e51b6eb655c72b2ca. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3559 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000054603 (LOC100404187) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000016087 CC High quality match with Homo sapiens transcript: ENST00000446105 CC (ING4-003) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000023867 (XM_001118270.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000006624 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000005726 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000010047 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000008479 (XM_001169091.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000004970 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RHTUQ01EVMJ3, F0X5I7101BAUV0, F0SMK5U02G1GPN, F1IUBWX02I180W CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:6823396-6823342. XX FH Key Location/Qualifiers FH FT source 1..230 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 230 BP; 65 A; 58 C; 61 G; 46 T; 0 other; tctccaggag aaacagattg agtcaagtga ctatgacagc tcttccagca aaggccggac 60 tcacaaggag aagaaagctg ctcgtgctcg ttccgaaggg aaaaactcag atgaagaagc 120 ccccaagact gcccagaaga agttaaagct cgtgcgcaca agtcctgagt atgggatgcc 180 ctcagtgacc tttggcagtg tccacccctc tgatgtgttg gatatgcctg 230 // ID JK123821; SV 1; linear; mRNA; EST; MAM; 430 BP. XX AC JK123821; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE14FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-430 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2769646ee43c8f5c3018f525b207378a. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3637 CC High quality match with Homo sapiens transcript: ENST00000416795 CC (BTN2A2-007) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000006682 (XM_001089758.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000042859 CC High quality match with Pongo abelii transcript: ENSPPYT00000018983 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1HESOY02JUAF4, F1RHTUQ02G3L5P, F0X5I7101EHRFL, F1IUBWX01D6RR6, CC F1HESOY02IOOAT CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:26567439-26567749. XX FH Key Location/Qualifiers FH FT source 1..430 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 430 BP; 134 A; 62 C; 98 G; 136 T; 0 other; cctgggacga agatgaatga agaacatgga ctcatgcgga tgtggtttgg cccacttgtc 60 cctgcaataa acaaggagtc agtacttagt ccctgagtgt ggttgaggtt tgaggtcctg 120 gttgagcagg gaagtactgg acctggtctg catcagactt caggttcaat ggggacacca 180 gtggcttcaa acttcctgat ctaattatgt ctttggacac ttagaaatta ttgaggacct 240 tagagagctt ttgttcattt gggttaatat ttgttaatat ttatggcatt tgacattgaa 300 acaaaaattt aaaatgttat cttttaattc atgttaaaat agcatgaata aatcaattat 360 aggttaatat aaataggatg ttttgtgaaa aagcaatcta ttgcgtccaa ataaaagaac 420 aagaagtgtg 430 // ID JK123822; SV 1; linear; mRNA; EST; MAM; 627 BP. XX AC JK123822; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE15FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-627 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 8de610aa17341781b0001fcec63767a7. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000024657 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000006709 CC High quality match with Homo sapiens transcript: ENST00000395969 CC (VPS41-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000014022 (XM_001101460.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000003850 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000035298 (XM_519057.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000020529 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1HESOY02IBGF4, F01W12W02I1GFJ, F0Q2XTE02JNC4R, FZOOKC302H9LVY, CC FZOOKC302HGWHQ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874246|FR874246.1:87657574-87657950. XX FH Key Location/Qualifiers FH FT source 1..627 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 627 BP; 159 A; 129 C; 131 G; 208 T; 0 other; gcaagactac aatttgcaaa ttctgcttcg tgaaggctgc aagaagattc tcgtagctga 60 ctctttgtcc ttactgaaga aaatgcaccg aactcagatg aaaggtgttc ttgttgatga 120 ggagaacatc tgtgagtcat gcctttcccc tgttcttcca tcagatgcag ctaagccctt 180 cagcgtggtg gtcttccatt gccggcacat gttccacaag gagtgcctgc ccatgcccag 240 catgaactct gctgcacagt tctgcaacat ctgcagcgct aagaaccgtg gaccaggaag 300 tgcaattttg gagatgaaaa aatagctcat ttctgcttgt cagtctcctc gtcaccactt 360 tttgagactg tttttacaac aacaaaagca tttgttgaca ctcgtgctgt taagagattt 420 gtttatgttt atattatgct cagaaacagt ttcttcatct attcctgtac taatggtttc 480 tctttgcagt tgacagagaa tttggggctc tcttcatgcc ttgaaatttt ggggtccata 540 gtccatatgt tgttatttat ttgtttggct cattctttat atagtaatgg aaacataagt 600 ctaggagtta gaaatgaatt ttttaga 627 // ID JK123823; SV 1; linear; mRNA; EST; MAM; 669 BP. XX AC JK123823; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE16FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-669 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 4bfa999adc58a971ced1360211647c84. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX02HGFBM, FZ8A1BE02I75CN, F1RTSFR01A8QPR, F01W12W02JBS6Q, CC F0DTF6N01DUQ5E CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874258|FR874258.1:93685129-93685198. XX FH Key Location/Qualifiers FH FT source 1..669 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 669 BP; 185 A; 135 C; 190 G; 159 T; 0 other; tggaaagggg cctcaggtgg ccacccctac ctggcatccc tctaatccag gaggatcgtg 60 gagaaagagg ctgtgggcct ctcggggagc gagctgcggg tagcggcgca ctggggacag 120 gcgcacgcct ggctgtcgcc cttgccgctg tgtttgggag gactcgaact ggcgccagga 180 aatattagga agctgtggtt ttcaaagcta attatgaaaa catttatcgt tggaatcagt 240 gggctctaca agaagcatgg tgctggtacg tgcttctgat gagggcctca ggaagcttac 300 attcatggtg gaaggagaag gggatccagc atgtcacatg acagaagaag gagcaagaga 360 gatgtgacaa acggtgggaa aacaacactg gctaagaatt tgcagaaaca cctcccaaat 420 tgcagtgtca tttctcagga tgattttttc aagccagagt ctgagataga gacagataaa 480 aatggatttt tgcagtatga tgtgcttgaa gctcttaaca tggaaaaaat gatgtcaacc 540 atttcctgct ggatggaaag cgcaagacgc tctgtggcat caacagaccg ggaaagtgct 600 gaggaaattc ccattttaat catcgaaggt tttcttcttt ttaattataa gccccttgac 660 actatatgg 669 // ID JK123824; SV 1; linear; mRNA; EST; MAM; 625 BP. XX AC JK123824; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE17FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-625 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2d84f828d991c4a7419b90d8dffea480. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1119 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000027372 (LOC100407057) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000012287 CC High quality match with Homo sapiens transcript: ENST00000407280 CC (IFI30-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000038657 (XP_001114116.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000019724 (XM_001162304.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01CHGQZ, F1C5L3D04I2HOP, F1BDRJG03FU6II, F0SMK5U02GUQMC, CC F0OTZ2I02GH35J CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/). XX FH Key Location/Qualifiers FH FT source 1..625 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 625 BP; 143 A; 175 C; 178 G; 129 T; 0 other; cctgccaccg ctgctgctgc tgctggacga ccccacggca gcggtgcagg cgtcccctct 60 gcaagcgtta gatatctttg ggaatgggcc gccagttaac tacaagatgg gcaatctata 120 cctgcggggg cccgtcaaga agtccaatgc accgcttgtc aacgtgaccc tctactatga 180 agcactgtgc ggtggctgcc gcgtcttcct gatccgagac cttttcccaa cgtggctgat 240 ggtcatggag atcctcaatg tcacgctggt gccctacgga aatgcacagg aacaaaatgt 300 cagcggcaga tgggagttca agtgccagca tggagaagag gagtgcaaac tcaacaaggt 360 ggaggcctgc ctgttggata tacttgacat ggatctagcc ttcctgaccg ttgtctgcat 420 ggaagagttt gacgacatgg agaaaagtct gccactatgc ctgcagctct acgccccagg 480 gctgtcgcca gacactatca tggagtgtgc aacgggggac cgcggcatgc agctcatgca 540 tgccaatgcc cagcggacag atgctcttca gccaccgcac gaatatgtgc cctgggtcac 600 cgtcaatggg aaacccttgg aagat 625 // ID JK123825; SV 1; linear; mRNA; EST; MAM; 599 BP. XX AC JK123825; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE19FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-599 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f9d3183a84777dc964e67f02bd3a3a43. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1593 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000062320 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000010999 CC High quality match with Homo sapiens transcript: ENST00000552419 CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000015816 (XM_001115506.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000008612 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X5I7101DKN8T, F1IUBWX02H6PQJ, F1IUBWX01AVARA, F1GITI001C41F8, CC F0OTZ2I01A8NZ6 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:32756383-32756314. XX FH Key Location/Qualifiers FH FT source 1..599 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 599 BP; 134 A; 165 C; 165 G; 135 T; 0 other; atcacagctg ctgcagtaaa gtctggggcc catagtttca tctctggact ccctcggggc 60 tatgccacag aggtaggtga ggctggaagc cagctgtctg ggggtcagcg acaggcagtg 120 gcgttggccc gagcattgat ccggaagcca tgtgtactta tcctggatga tgccaccagt 180 gccctggatg caaacagcca gttacgggtg gagcagctcc tgtacgaaag ccctgagcgg 240 tactctcgct cagtgcttct catcacccag aacctcagcc tggtggagca ggctgaccac 300 atcctctttc tggaaggagg cactatccgg gaggggggaa cccaccagca gctcatggag 360 aaaaaggggt gctactgggc catggtacag gctcctgcag acgctccaga atgaaagcct 420 tctcagacct gcacactcca tctccctccc ttttcttctc tctgtggtgg agaaccacag 480 ctgcagagta ggcagctgcc tctagaagga gttacttgaa attttacttt gagtgtgtta 540 cctcctttcc aagctcctca tgataatgca gacttcctgg aggacaaaca caggatttg 599 // ID JK123826; SV 1; linear; mRNA; EST; MAM; 632 BP. XX AC JK123826; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE20FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-632 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 5d14323c451b2f1bab08fca8107547c9. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2553 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H02JZ5J2, F0DTF6N01BCJNW, F0SMK5U02ID4L5, F0OTZ2I01EH70F, CC F0Q2XTE01EEFD9 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874259|FR874259.1:73052230-73052359. XX FH Key Location/Qualifiers FH FT source 1..632 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 632 BP; 121 A; 181 C; 186 G; 103 T; 41 other; gggaggccag gcgagcctga ctctgttgtc ctacccatgc ctgggcccct cccctcaggg 60 cccatggtaa cgaaccccta ggaagaagag actgggtgtt aggggtgcac agtccacagt 120 gagctgaaga gcgaggtttc atggcaacat ggcccggccc ctcaccctcc gtccccacaa 180 caggacccgt ggggcctgcc tttgcagggc acagatgacc aaagtccctt cctgcttcct 240 gttccctgtc ttactctggg gagaaagagg ggcctgatga gactccacac gggtagacac 300 atcaccaggg gcatctgcag gccccgggct ggctgcttgt agccaggaga agggcaacgg 360 gagggagcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc atggaaatcc 420 gggcattgga tgtctgactc cctccccacc caaaagggga aggacccctc accacctcca 480 tcggcgggac agtttacttg ccaactcgcc gtgtttttgc caaaaccaag attttgaagg 540 aaatgagtgg ccagcgccag agcccaggcc gtgtggcctg cccagcctca gcgtcactcg 600 gtggcagggt ggggtggggg gtgggcagca gc 632 // ID JK123827; SV 1; linear; mRNA; EST; MAM; 670 BP. XX AC JK123827; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE21FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-670 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; ae5d7c7f880a4231886864f5f748c877. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000013731 (TLE3) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000033844 CC High quality match with Homo sapiens transcript: ENST00000451782 CC (TLE3-204) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000019683 (XM_001086565.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000017354 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000013345 (XM_001174941.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000007789 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0OTZ2I02GFPIA, F1GITI002FJ339, F0ZWYBK01AWTKU, F0ZWYBK02I5ORN, CC FZ8A1BE02H0ALD CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:48971783-48971667. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 670 BP; 138 A; 187 C; 196 G; 149 T; 0 other; gctggccgtg ggcatggaga gcagcaacgt ggaggtgctg caccacacca agcctgacaa 60 gtaccagctg cacctgcacg agagctgtgt gctctccctc aagttcgcct actgcggcaa 120 gtggttcgtg agcactggga aagataacct tctcaacgcc tggaggacgc cttatggagc 180 cagcatattc cagtctaaag aatcctcgtc tgtcttgagt tgtgacattt cggcggatga 240 caaatacatt gtaacaggct ctggtgacaa gaaggccaca gtttatgagg tcatctacta 300 aacaagaact ccagcagggc tgtcaaactc tgggagaaac cgactcggct ctgacaggga 360 gacccccagg cgaggggccc tgaggatggc ggaggatggg ccgcaggcag ccgagcgttc 420 ggggctgcgc tccggccggc tgagagggcg cgtgccgcat cacagtctgg acttctgggc 480 ctagattgat gtgtctcaca gactcggatg ggttctgctc ctcctcctcc ccctgaacaa 540 tgctggcagt tgctacaaat agatttattg gaggcttatg gctccggttc cccacagacc 600 cgctcatgag tctctttgtt cttccccttt cttttgccct gtgccccacc ttgggtcggg 660 gatgctggag 670 // ID JK123828; SV 1; linear; mRNA; EST; MAM; 532 BP. XX AC JK123828; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE22FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-532 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 306a24f97b87cf841a758e9457a82e79. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Homo sapiens transcript: ENST00000358127 CC (PAX5-001) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RHTUQ02HD42I, F0DRJ6O01CJIRP CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874258|FR874258.1:40611243-40611771. XX FH Key Location/Qualifiers FH FT source 1..532 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 532 BP; 151 A; 136 C; 121 G; 124 T; 0 other; cctgtggcag gagagacaga ggcacaaacc cacccaccca tcagcttcgg gtggctgatc 60 agggcccagg gagagaagca ggtaaaacag cagggtgggg gtgacttggc agtcatgcac 120 ccctccccta ctctgaggcc tggcaggagg aagtcacgcc aggaggaggc cacaccagcc 180 tcacctgcct tgacccccac cccctgcccc acgaccctgt ggctatggct gctggtcgct 240 cgtgtcccca aaatcaccat gcctatggcc acgtccctca tcttctccaa aaacatcatt 300 aagaacaagt gattttggat gatggatttt tgatttacaa atggtgcatt tcccttggag 360 tggaacagag aggaaaccat ttaatggcgc ccttcttttc aagcatgaat acattttaat 420 gaaactattt tattgtattt gaggaaatgg agagttgaac attccaacca atcaatagcc 480 aattaattgc tataaagcta aaaagaaaat aaataaatcc tgagtctatt tt 532 // ID JK123829; SV 1; linear; mRNA; EST; MAM; 697 BP. XX AC JK123829; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE23FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-697 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 34e2ee4665c03059e3bd1b5919756790. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1177 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000020616 (A6MJV5_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000014514 CC High quality match with Homo sapiens transcript: LRG_76_t1 CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000001358 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000003102 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000062457 (NP_001029294.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX01AXR24, F0DTF6N01D26RL, F0ZWYBK02I06MT, F0BWDJC02IV79A, CC F0BWDJC02GKMAN CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874246|FR874246.1:1845551-1845847. XX FH Key Location/Qualifiers FH FT source 1..697 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 697 BP; 148 A; 196 C; 221 G; 132 T; 0 other; ccggctgctc ctcgccctgc ggcgagcaca tttcctgtgc tgagtgcctg aagttcgaca 60 aaggcccttt cgggaagaac tgcagcgcgg cgtgtcggcg cctgcagctg tcgaacaacc 120 ccgtgaaggg caggacctgc aaggagaggg actcagaggg ctgctgggtg acgtacatgc 180 tggagcagca ggacgggtgg gatcgctaca tcatctatgt ggatgagagc cgagagtgcg 240 tggcgggccc caacatcgcc gccatcgttg ggggcactgt ggcgggcatc gtgctgatcg 300 gcgtcctcct gctggtcatc tggaaggccc tgacccacct gagcgacctc cgggagtaca 360 ggcgttttga gaaggagaag ctcaagtccc agtggaacaa cgataatccc cttttcaaga 420 gtgccaccac gacggtcatg aaccctaagt ttgctgagag ttaggagcac ttggtgaaga 480 caaggccgtc gggccctcca cgtctgcccc gtcacgcggc cgagacatgg cttgcaacag 540 ctcttgagga tgtcaccaat taaccagaaa tccagttatt ttctgccctc aaaatgacag 600 ccatggccag ccgggtgctt ctaggggctc atcagggggg acagccctgc tctgacctgc 660 acagtcctgg catggagacg tgagaaggaa ggcttga 697 // ID JK123830; SV 1; linear; mRNA; EST; MAM; 687 BP. XX AC JK123830; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YE24FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-687 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f7d8627c91aa19ac5c8358936a4812e4. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig83 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000034890 (LOC100394454) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000028677 CC High quality match with Homo sapiens transcript: ENST00000479563 CC (RPL14-003) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000008952 (LOC719408) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000009217 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000007253 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000000278 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000066657 CC High quality match with Pongo abelii transcript: ENSPPYT00000005737 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000002161 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H01D48WE, FZ8FYPF02JBEBZ, FZ8FYPF02JS8Z1, F1IUBWX02JO0F7, CC FZ8FYPF02FL2A0 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874264|FR874264.1:120474844-120475508. XX FH Key Location/Qualifiers FH FT source 1..687 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 687 BP; 231 A; 151 C; 169 G; 135 T; 1 other; cttcgtggag gttggccggg tggcctacgt ctcctttgga cctcatgccg gaaaattggt 60 cgcgattgta gatgttattg atcagaacag ggctttggtc gatggacctt gcactcaagt 120 gaggagacag gccatgcctt tcaagtgcat gcagctcact gatttcatcc tcaagtttcc 180 acacagtgcc cgccagaagt atgtccgaca agcgtggcag aaggcagaca tcaatacaaa 240 atgggcagcc acacgatggg ccaagaagat tgaagccaga gaaaggaaag ccaagatgac 300 agattttgat cgttttaaag ttatgaaggc aaagaaaatg aggaacagaa taatcaagaa 360 tgaagttaag aagcttcaaa aggcagctct cctgaaagct tctcccaaaa aagcgcctgg 420 tactaagggt gctgctgctg ctgctaaagt tccagcaaaa aagatgacca ccgcgagtaa 480 aaaggctcca gcccagaagg ttcctgccca gaaagccaca ggccagaagg ctgcgcctgc 540 tccaaaagct cagaagggtc aaaaagctcc agcccagaaa gcacctgctc caaaggcatc 600 tggcaagaaa gcataagtgg caatcaaaaa aagtaataaa ggttcttttt gacatgttgg 660 caaatgttan aaaaaaaaaa aaaaata 687 // ID JK123831; SV 1; linear; mRNA; EST; MAM; 369 BP. XX AC JK123831; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF01FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-369 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 82ac5edd072d33ad864df14956389c3a. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1804 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000014381 (TKT) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000032168 CC High quality match with Homo sapiens transcript: ENST00000469678 CC (TKT-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000020254 CC High quality match with Microcebus murinus transcript: CC ENSMICT00000010918 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000009220 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000028131 (TKT_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000029374 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z1IVZ01B8P8V, F0X5I7102JHF6W, F1IUBWX02HFSJV, F1GITI002FLTKP, CC F0Z1IVZ01DPM9F CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874245|FR874245.1:83364570-83364764. XX FH Key Location/Qualifiers FH FT source 1..369 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 369 BP; 73 A; 112 C; 112 G; 72 T; 0 other; gctggctttc tgcccgaggc agagctgctc aacctgagga agatcagctc cgacttggat 60 gggcacccgg tccccaaaca agctttcacc gatgtggcca ctggctccct gggccagggc 120 ctcggggccg cttgtgggat ggcctacacc ggcaaatact tcgacaaggc cagctaccga 180 gtctattgct tgctgggaga tggggagctg tcagagggct ccgtatggga ggccatggcc 240 tttgccagca tctataagct ggacaacctc gtggccattc tagacatcaa tcgcctgggc 300 cagagtgacc cacccccgct gcatcaccag atggacatct accagaagcg gtgcgaggcc 360 ttcggttgg 369 // ID JK123832; SV 1; linear; mRNA; EST; MAM; 677 BP. XX AC JK123832; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF02FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-677 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 5ae1d8cd99f2f9299c343e7f33e8ff90. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3126 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02JU0AD, F0SMK5U02GE66G, F0M2C4T02GUYJL, F0BOF8H02HBL89, CC F0ZWYBK02J4EMX CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:12557813-12557707. XX FH Key Location/Qualifiers FH FT source 1..677 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 677 BP; 172 A; 182 C; 179 G; 144 T; 0 other; ggaagtgaca tcgtctttaa accctgcgtg gcaatccctg acgcaccgcc gtgatgccca 60 gggaagacag ggcgacctgg aagtccaact acttccttaa gatcatcata aacaggctca 120 ggcaagttaa gtggctccta agatgacagc attcatatcc agcaactatt ggatgattat 180 ccgaaatgtt tcatcgtggg agcagacaat gtgggctcca agcagatgca gcagatccgc 240 atgtcccttc gagggaaggc cgtggtgctg atgggcaaga acaccatgat gcgcaaggcc 300 atccgagggc acctggaaaa caacccagct ctggagaaac tgctgcctca tatccgggga 360 aatgtgggct ttgtgttcac caaggaggac ctcactgaga tcagggacat gttgctggcc 420 aataaggtgc cagctgctgc ccgtgctggt gccattgctc catgtgaagt cactgtgcca 480 gcccagaaca ctggacttgg gcccgagaag acctcctttt tccaggcttt aggtatcacc 540 actaaaatct ccaggggcac cattgaaatc ctgagtgatg tgcagctgat caagactgga 600 gacaaagtgg gagccagcga agccacgctg ctgaacatgc tcaacatctc ccccttctcc 660 ttttggctgg tcatcca 677 // ID JK123833; SV 1; linear; mRNA; EST; MAM; 531 BP. XX AC JK123833; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF03FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-531 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 850571e619c68b1258d42170c8d16bc4. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2863 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000003099 (Q257X3_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000030281 CC High quality match with Homo sapiens transcript: ENST00000399450 CC (AL662842.1-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000040927 (Q30706_MACMU) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000001215 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000065607 (Q95507_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000019175 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H02GLUW3, F0BOF8H01AD0FK, F0Q2XTE02FWC5T, F1RHTUQ02GZGG5, CC F1HESOY01C02ED CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:32497008-32496942. XX FH Key Location/Qualifiers FH FT source 1..531 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 531 BP; 122 A; 135 C; 152 G; 122 T; 0 other; ctcctggtct gctctgtgag tggtttctat ccaggcagca ttgaagtcag gtggttccgg 60 aacggtgagg aagagaaggc tggggtggtg tccacaggcc tgatccagaa tggagactgg 120 accttccaga ccctggtaat gctagaaaca gttcctcaga gtggagaggt ttacacctgc 180 caagtggagc acccaagcgt gaccagccct ctcacagtgc aatggagagc acggtctgaa 240 tctgcacaga gcaagatgct gagtggagtc gggggctttg tgctgggcct gctcttcctt 300 ggggccgggc tgttcatcta cttcaggaat cagaaaggac actctggact tcagccaaca 360 ggactcctga gctgaagtgc agatgaccac attcaaggaa gaaccttctg ccccagcttt 420 gcaggatgaa aatttcccac ctggctgtta ttctgccaca agaaagggct ttctcaggac 480 ctggttgcta ctggttcagc aactgcagaa aatgccctcc cttgtggctt c 531 // ID JK123834; SV 1; linear; mRNA; EST; MAM; 466 BP. XX AC JK123834; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF04FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-466 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 10fbb5b7998fa6b842796f873540141a. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig252 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000025650 CC High quality match with Homo sapiens transcript: ENST00000392884 CC (CD3D-003) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000067708 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01DJ01I, F1GITI002HGR8W, FZOOKC302FQPCQ, F0Q2XTE01BVCKN, CC FZ8A1BE02GO7PE CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:117607709-117607661. XX FH Key Location/Qualifiers FH FT source 1..466 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 466 BP; 137 A; 112 C; 108 G; 109 T; 0 other; catagcatgt ttctgtctgg actggtactg gctacccttc tctcccaagt gagccccttc 60 gagatacctg tagaggaact tgaggacaga gtgtttgtga aatgcaatac cagcatcaca 120 tgggtagagg gaacggtggg aacactgctc acaaataata caagactgga cctgggaaaa 180 cgcatcctgg acccacgagg aatatatagg tgtaatggga cagatatata caaggacaaa 240 gaatctactg tgcaagttca ttatcgaact gccgacacac aagctctatt gaggaatgac 300 caggtctatc agcccctccg agatcgagat gatgctcagt acagccgcct tggaggaaac 360 tgggctcgga acaagtgaac ctgagactga tggcttctag aagcatccat taccaactgt 420 accttccctt cttgctcagc caataaatat atcctctttt actcag 466 // ID JK123835; SV 1; linear; mRNA; EST; MAM; 630 BP. XX AC JK123835; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF05FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-630 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; c6949327ebd4bee401c113fe489c9d65. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2044 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000017202 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000025682 CC High quality match with Homo sapiens transcript: ENST00000551150 CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000045510 (Q9GMG8_MACMU) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000012550 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000000394 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000003846 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000010142 (XR_020374.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000005944 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000009733 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0SMK5U02GE66G, F0ZWYBK02JU0AD, F0M2C4T02GUYJL, F0ZWYBK02J4EMX, CC F0BOF8H02HBL89 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:12557783-12557154. XX FH Key Location/Qualifiers FH FT source 1..630 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 630 BP; 157 A; 171 C; 170 G; 132 T; 0 other; gcaatccctg acgcaccgcc gtgatgccca gggaagacag ggcgacctgg aagtccaact 60 acttccttaa gatcatccaa ctattggatg attatccgaa atgtttcatc gtgggagcag 120 acaatgtggg ctccaagcag atgcagcaga tccgcatgtc ccttcgaggg aaggccgtgg 180 tgctgatggg caagaacacc atgatgcgca aggccatccg agggcacctg gaaaacaacc 240 cagctctgga gaaactgctg cctcatatcc ggggaaatgt gggctttgtg ttcaccaagg 300 aggacctcac tgagatcagg gacatgttgc tggccaataa ggtgccagct gctgcccgtg 360 ctggtgccat tgctccatgt gaagtcactg tgccagccca gaacactgga cttgggcccg 420 agaagacctc ctttttccag gctttaggta tcaccactaa aatctccagg ggcaccattg 480 aaatcctgag tgatgtgcag ctgatcaaga ctggagacaa agtgggagcc agcgaagcca 540 cgctgctgaa catgctcaac atctccccct tctcctttgg gctggtcatc cagcaggtgt 600 ttgacaatgg cagcatctac aatcctgaag 630 // ID JK123836; SV 1; linear; mRNA; EST; MAM; 550 BP. XX AC JK123836; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF06FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-550 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 951819170984e30d6a41fd1548ef772a. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig117 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000060108 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000033635 CC High quality match with Homo sapiens transcript: ENST00000457903 CC (CR759828.1-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000041082 (B0JDT6_MACMU) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000007708 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000011714 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000067873 (Q9MXL5_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000020040 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0DTF6N01D972I, F0Z8TLB01A87CQ, FZ8FYPF02GONMF, F1HESOY02IGKBE, CC F0X5I7101E0GL9 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:31155176-31155079. XX FH Key Location/Qualifiers FH FT source 1..550 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 550 BP; 99 A; 182 C; 191 G; 78 T; 0 other; gccgagatgc gggtcatggc gccccgaacc ctgctcctgc tgctctcggg ggccctggcc 60 ctgaccgaga cctgggccgg ctcgcactcc ttgaggtatt tcagcaccgc cgtgtcccgg 120 cccggccgct gggagcccca cttcatctcc gtgggctacg tggacgacac gcagttcgtg 180 cggttcgaca gcgacgccga gagtccgacg atggagccgc gggcgccgtg ggtggagcag 240 gaggggccgg agtattggga ccggaacaca cggagagtca agggccacgc acagactgac 300 cgaaggaacc tgcggaccct gctccgctac tacaaccaga gcgagtgggg gtctcacaca 360 gtccagagga tgtttggctg cgacctgggg cccgacgggc gcctcctccg cgggtatcac 420 cagtccgcct acgacggcaa ggattacatc gccctgaacg aggacctgcg ctcctggacc 480 accgcggaca tggcggctca gaacacccag cgcaagtggg aggcggccgg tactgcggag 540 cagctgagag 550 // ID JK123837; SV 1; linear; mRNA; EST; MAM; 499 BP. XX AC JK123837; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF07FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-499 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; d3cea89157af6906dbbf4a316f873b8b. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1385 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000028370 (Q58YJ5_9PRIM) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X19WM01A5WMM, F1IUBWX01D6U23, F0OTZ2I02H6WVL, FZ8A1BE01DHXQ0, CC F0Q2XTE01DGZJC CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874251|FR874251.1:108785530-108785075. XX FH Key Location/Qualifiers FH FT source 1..499 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 499 BP; 156 A; 143 C; 67 G; 133 T; 0 other; tctaatcctg attgccctac catctctacg cattctgtac ctaacagacg aaatcaacaa 60 cccctccttt actattaaat caattggaca tcaatgatac tgaacctacg aatacacaga 120 ctacggaggc ctcatcttta attcttacat actgcctcca ttattcttaa acccaggaga 180 ccttcgactt ctagaagttg acaaccgagt ggttctccca attgaagccc ccgtccgtat 240 aataattaca tctcaagatg tcctacactc atgaactatt cccacactag gtttaaaaac 300 agatgcagta cccggacgct taaatcaaac cacatttacc gccacacgac caggcgtcta 360 ttatggacaa tgctcagaaa tctgtggggc taaccacagc ttcataccta tcgtcgcaga 420 actaatccca ttaaaaatct ttgaaatagg acctgtgttt accctataaa taccccactc 480 cctttccact atacaaatg 499 // ID JK123838; SV 1; linear; mRNA; EST; MAM; 417 BP. XX AC JK123838; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF08FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-417 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2a7ca7ebbe2c5409825e73fe2965d4a7. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig152 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000000243 (LOC100407446) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000031482 CC High quality match with Homo sapiens transcript: ENST00000497965 CC (RPL22-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000025784 (XM_001090986.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000013216 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000000172 (XR_020665.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000002330 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000003641 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0DTF6N02FQBHX, F0M2C4T02F72ON, F01W12W02F5UO3, F1HESOY02ILCN2, CC FZ8A1BE02JVQJP CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:134547716-134547302. XX FH Key Location/Qualifiers FH FT source 1..417 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 417 BP; 141 A; 67 C; 109 G; 100 T; 0 other; ggcgaagggg ggcaaaaaaa agaagcaggt tctgaagttc actcttgatt gcacccaccc 60 tgtagaagat ggaatcatgg atgctgccaa ttttgagcag tttttgcaag aaaggatcaa 120 agtgaacgga aaagctggga accttggtgg aggggtggtg accatcgaaa ggagcaagag 180 caagatcacc gtgacatctg aggtgccttt ctccaaaagg tatttgaaat atctcaccaa 240 aaaatatttg aagaagaata atctacgtga ctggttgcgc gtagttgcta acagcaaaga 300 gagttacgaa ttacgttact tccagattaa ccaggacgaa gaagaggagg aagatgagga 360 ttaaatttca tttatccgga atattttgta tgagttcttg aataaaactt gggaacc 417 // ID JK123839; SV 1; linear; mRNA; EST; MAM; 563 BP. XX AC JK123839; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF09FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-563 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; e4009ce8d8eb32b336a0954e08554665. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Homo sapiens transcript: ENST00000534355 CC (C11orf31-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000003648 (NP_001152973.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000045384 (XR_020651.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF02FQU03, F1HESOY01C40F9, F1HESOY01C40F9, FZ8FYPF02FQU03, CC F1HESOY02FNJCT CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:15813113-15812994. XX FH Key Location/Qualifiers FH FT source 1..563 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 563 BP; 134 A; 134 C; 187 G; 108 T; 0 other; ggctccccgc ggtaggaagc ggaaggctga ggccgcgatg gtcgccgcag ccgagaagcg 60 agagaagctg gcaaacagcg gggagggaat ggaggaggca accgttgtta tcgagcattg 120 cacgagctga cgcgtctatg ggcgcaacgc cgcggccctg agtcaggcgc tgcgcctgga 180 ggccccagag cttccagtaa aggtgaaccc gaccaagccc cggaggggca gcttcgaagt 240 gacgctgctg cgcccggacg gcagcagtgc ggagctctgg actgggatta agaaggggcc 300 cccacgcaaa ctcaaattcc ctgagcctca ggaggtggtg gaagagttga agaagtacct 360 gtcgtaggga gatttgggta gaagtcctca tgctgagctt tgtgtccctg gtgatgttgg 420 aacattaatg atggaacatg gccaaacttc agtcatgtgc ctgaagccat ggtttcttcc 480 ccgccagaaa tgaaggttca gttgtgaggc aaccctctag taaggcattg caaaagttac 540 tggatttggt ttaataaaag ttg 563 // ID JK123840; SV 1; linear; mRNA; EST; MAM; 644 BP. XX AC JK123840; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF10FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-644 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; eb39f6aae2a6a889dd2bfbe06f0c5560. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000000864 (LOC100409142) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000010750 CC High quality match with Homo sapiens transcript: ENST00000272930 CC (UBE2F-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000025988 (XM_001088736.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000014256 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000024261 (XM_001154160.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000015489 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000011509 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BWDJC01C3SGH, F1RHTUQ01DRKVR, F1RHTUQ01DE0AS, FZOOKC302F0EI6, CC F1HESOY01CQQUF CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:13570030-13569387. XX FH Key Location/Qualifiers FH FT source 1..644 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 644 BP; 182 A; 136 C; 170 G; 156 T; 0 other; gggcatggtg ttgggcgccg gacccgcctc gcctgtttcg gggagcccag ggtaaaggca 60 gcagtaatgc taacgctagc aagcaaactg aagcgtgacg atggtctcaa agggtcccgg 120 acggcagcca cagcgtccga ctcaactcgg agggtttctg tgagagacaa attgcttgtt 180 aaagaggttg cagaacttga agctaattta ccttgtacat gtaaagtgca ttttcctgat 240 ccaaacaagc ttcattgttt tcagctaaca gtaaccccag atgagggtta ctaccagggt 300 ggaaaatttc agtttgaaac tgaagttccc gatgcgtaca acatggtgcc tcccaaagtg 360 aaatgcctga ccaagatctg gcaccccaac atcacagaga caggggaaat atgtctgagt 420 ttactgagag aacattcgat tgatggcact ggctgggctc ccacaagaac attaaaggat 480 gtcgtttggg gattaaactc tttgtttact gatcttttga attttgatga tccactgaat 540 attgaagctg cagaacatca tttgcgggac aaggaggact tccggaataa agtggacgac 600 tacatcaagc gttatgccag atgatataag gggacgattg cagg 644 // ID JK123841; SV 1; linear; mRNA; EST; MAM; 228 BP. XX AC JK123841; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF11FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-228 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; b7d3cea0a89b6f91b2a62787094f9173. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig725 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000054323 (LOC100401730) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000033877 CC High quality match with Homo sapiens transcript: ENST00000375114 CC (RALY-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000025415 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000012821 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000024914 CC High quality match with Pongo abelii transcript: ENSPPYT00000012688 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1HESOY02H34PG, F1RHTUQ01BIER7, F1GITI002H7WCC, F1IUBWX02IFAKC, CC F1HESOY01C68J8 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874253|FR874253.1:30496210-30495993. XX FH Key Location/Qualifiers FH FT source 1..228 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 228 BP; 41 A; 79 C; 59 G; 49 T; 0 other; cctcccccta cgcatcccag ccagtgccat gtcctctgca ggtggagtta ctggcctact 60 ccttccccat gagccctccc tgtctgcact gcccaggcca gagggtagag cacaggagtt 120 tccccacact gcctcccctc cccaggacac tcctaggctt gggttttttc tataggtttg 180 gcggggggcc acagggaggg gaccctgaca ataaagagat tggatccc 228 // ID JK123842; SV 1; linear; mRNA; EST; MAM; 664 BP. XX AC JK123842; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF12FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-664 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 2b6de09b4fd2ea5f370cb984d5b84079. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1572 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000041261 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000011754 CC High quality match with Homo sapiens transcript: ENST00000353334 CC (CD74-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000012750 (XM_001099491.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000013239 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000004631 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000032219 (XM_001166096.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000018564 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1GITI001CGYHI, FZ8A1BE01ESJAS, F0X19WM01BJ015, F0X19WM01DF2PQ, CC F0M2C4T01AHCF5 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874249|FR874249.1:147786303-147786183. XX FH Key Location/Qualifiers FH FT source 1..664 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 664 BP; 163 A; 202 C; 190 G; 109 T; 0 other; acaggagcag caggaggagc tgtcaggaag atcagaagcc agtcatggat gaccagcgcg 60 acctgatctc caacaatgag caactgccca tgctgggccg gcgccctgga accccggaga 120 gcaagtgcag ccgtggagcc ctgtacacag gcttttccat cctggtgact ctgcttctgg 180 ctggccaggc caccaccgcc tacttcctgt accagcagca gggccggctg gacaaactga 240 cagtcaccac ccagaacctg cagctggaga acctgcgcat gaagcttccc aagcctccca 300 agcctgtgag caagatgcgc atggcaaccc cgctgctgat gcaggcgcta cccatgggag 360 cccaggggcc catgcagaat gccaccaagt atggcaacat gacggaggac catgtgatgc 420 acctgctcca gaatgctgac cccctgaagg tgtacccgcc gctgaagggg agcttcccgg 480 agaacctgag acaccttaag agcaccatgg agaccttgga ctggaaggtc tttgagagct 540 ggatgcacca ttggctcttg tttgaaatga gcaagcactc cttggagcaa aagcccactg 600 aggctccacc aaaagagtca ctggaactgg aggacccgtc ttccgggctg ggtgtgacca 660 aaca 664 // ID JK123843; SV 1; linear; mRNA; EST; MAM; 502 BP. XX AC JK123843; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF13FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-502 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1197ae61413d2dabc7013fe24ab6d900. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2083 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000042575 (LOC100399899) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000006602 CC High quality match with Homo sapiens transcript: ENST00000457341 CC (CR759817.2-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000046042 (LOC723529) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000008716 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000006228 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000033347 (XM_001170296.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000029432 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZOOKC302FNV6R, F1IUBWX01C72YU, F0Z1IVZ02F9BVV, F0M2C4T02HGAJY, CC F0Z8TLB01DQ762 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874249|FR874249.1:177391333-177390833. XX FH Key Location/Qualifiers FH FT source 1..502 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 502 BP; 139 A; 119 C; 141 G; 103 T; 0 other; gctgccgcca tgtctctagt gatccctgaa aagttccagc atattttgcg agtactcaac 60 accaacatcg atgggcggcg gaaaatagcc tttgccatca ctgccattaa gggtgtgggc 120 cgaagatatg ctcatgtggt gttgaggaaa gcagacattg acctcaccaa gagggcggga 180 gaactcactg aggatgaggt ggaacgtgtg atcaccatta tgcagaatcc acgccagtac 240 aagatcccag actggttctt gaacagacag aaggatgtaa aggatggaaa atatagccag 300 gtcctagcca atggtctgga caacaagctc cgtgaagacc tggagcgact gaagaagatt 360 cgggcccata gagggctgcg ccacttctgg ggccttcgtg tccgaggcca gcacaccaag 420 acgactggcc gccgtggccg caccgtgggt gtgtccaaga agaaataagt ctgtaggcct 480 tgtcaataaa tagtttatat ac 502 // ID JK123844; SV 1; linear; mRNA; EST; MAM; 610 BP. XX AC JK123844; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF14FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-610 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f60ba3ffe91ce3c378d4f491a42f35bb. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1560 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000037225 (A6MJV4_CALJA) CC High quality match with Homo sapiens transcript: ENST00000329251 CC (EEF1G-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000009232 (XR_012977.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000009831 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000004759 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000007024 (XR_023872.1) CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000000145 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T02ICKFW, F0DRJ6O01CDAVD, F0ZWYBK02IIAFD, F1GITI001BUZVL, CC F1IUBWX02GKE9W CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:45678706-45679306. XX FH Key Location/Qualifiers FH FT source 1..610 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 610 BP; 132 A; 175 C; 163 G; 140 T; 0 other; gaatcaccat ggcggctggg accctgtata cgtatcctga aaactggagg gccttcaagg 60 ctctcatcgc tgctcagtac agcggggctc aggtccgcgt gctctccgca ccaccccact 120 tccattttgg ccaaaccaac cgcacccctg aatttctccg caaatttcct gccggcaagg 180 ttccagcatt tgagggtgac gatggattct gtgtgtttga gagcaatgcc attgcctact 240 atgtgagcaa tgaggagctg cggggaagta ctccagaggc agcagcccag gtggtgcagt 300 gggtgagctt tgctgattcc gacatagtgc ccccagccag tacctgggta ttccccacct 360 tgggcatcat gcaccacaac aaacaggcca ctgagaatgc aaaggaggaa gtgaggcgaa 420 ttctggggct gctggatgct cacttgaaga cgaggacttt tctggtgggc gaacgagtga 480 cattggctga catcacagtt gtctgcaccc tgttgtggct ctataagcag gtcctagagc 540 cttctttccg ccaggccttt cccaatacca accgctggtt cctcacctgc attaatcagc 600 cccagttccg 610 // ID JK123845; SV 1; linear; mRNA; EST; MAM; 703 BP. XX AC JK123845; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF15FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-703 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; c7b43c4c9a76847390eec8741af6032c. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZOOKC302J09GM, F0DRJ6O02GLGL0, F0Z1IVZ01BV3KD, F0M2C4T01E51XW, CC F0BOF8H02HW6AZ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:229388469-229388197. XX FH Key Location/Qualifiers FH FT source 1..703 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 703 BP; 222 A; 133 C; 198 G; 150 T; 0 other; taaaaacaaa aaaaaattag ctgggcgtgg tggcgggtcc ctgtagtccc agctactcgg 60 gaggctaagg caggagagaa tggcgtgaat ccgggaggtg gagcttgcag tgagccgaca 120 tctggtcact gcactccagc ctgggcgaca aagggagact aaatggaatg aatgaatgaa 180 taaacaaaaa ctgccaggca aggtggctca cgcctgtaat cctggcactt tgggaggcca 240 aggtgggtgg atcacgagtt cgagaccagc ctgaccaatg tggtgaaacc ctttctctac 300 taaaagtaca aaaaactagc cgggcatggt ggtgcttccc tgtagtccca gctactcggg 360 agactgaggc aggagaatca cttgaatccg ggaggcagag gttgcagtga actgagatca 420 cgccactgta ctccagcctg ggcaacagag caagactcca tctcaaaaag aaatacggaa 480 ctaacttatg ataagttttg gaatcagaag tactctcatg ataaaacggg tataaaaggg 540 aaggtagatg agaagcatgt gataaaaaac cagttcacca tattatacta ctggataagg 600 cagaggttta catactgtgt aaatgggatt cagagtgaga ctaggctggg atggagtatt 660 tggattgaac atgattctgc ttataagaat gagagtgtta aat 703 // ID JK123846; SV 1; linear; mRNA; EST; MAM; 689 BP. XX AC JK123846; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF16FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-689 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 512fa8e371c274ae63b2390eab730399. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3647 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000012844 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000009054 CC High quality match with Homo sapiens transcript: ENST00000375105 CC (PLA2G2D-001) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000000559 (XM_524585.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE02HOUZY, F0X5I7102GK55G, F0Q2XTE01CDMNE, F1RHTUQ01AGO88, CC FZ8FYPF02IWDTY CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:22744198-22743514. XX FH Key Location/Qualifiers FH FT source 1..689 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 689 BP; 153 A; 210 C; 156 G; 170 T; 0 other; atagtctcgg aactacaacc ggagcagcca ctttaaaggg caatattgat ttttctgtcc 60 atgtaactct ctctcttaaa atctcaaggc cctccactgt cctaagacaa agcctctcat 120 aggcgttggg gaccctgcac cgtccggcca tgtgaccttc tccccaggca agctctgaag 180 tccctgcagg tggaggccat gcctgtctta aactcagttg catccctggt gcccaaaaca 240 acactcagaa ccaaggagct ccgtaaatcc gtcttgggcg aagcgctaga caaagccgcc 300 agctcatatg gctccgggca ccagagcctt gagtactttc tcctgattcc aggcgtcagc 360 tcagggtgaa ttacaagggg ctactgaatg gctattactt tcatcacgac tgatccccat 420 ctcctcaggg tcaaagggct actttctgga agtctccccg ggctgactcc tcctccctga 480 ctgcaagggc tcactccctc ctccaagttc ccacaatgct tcatggctct gctgctgacc 540 aagcttggcc tagagtggca aatggaattt ctctgatctc ccccaacgaa actggaaccc 600 tgaaggacgg aggccatgtg tgtgctgtct ctgttttccc ctgtttcccc acatactggg 660 tgctcaattc atgcctgtga atggagtga 689 // ID JK123847; SV 1; linear; mRNA; EST; MAM; 143 BP. XX AC JK123847; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF17FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-143 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; ec27538ef3cdc952c3f4d8c977c35005. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1149 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000060392 (O78021_CALJA) CC High quality match with Homo sapiens transcript: ENST00000413089 CC (CR933859.2-003) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000040927 (Q30706_MACMU) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000008534 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000066472 (Q1JUK5_PANTR) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RTSFR01BYCZH, F0BOF8H02HTAL5 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:32605474-32605354. XX FH Key Location/Qualifiers FH FT source 1..143 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 143 BP; 27 A; 39 C; 39 G; 38 T; 0 other; catcaggttc gagctgtatt gactaccact acttttccct tcgtctcaat tatgcctcgg 60 aagaaggctt tgcggatccc cggaggcctt tgggggacaa ctgtgacctt gatgctggcg 120 atgctgagca ccccagtggc tga 143 // ID JK123848; SV 1; linear; mRNA; EST; MAM; 346 BP. XX AC JK123848; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF18FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-346 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1f25a805f90a62735cc4e2f0f1dc8ea3. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000039676 (LOC100408513) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000013093 CC High quality match with Homo sapiens transcript: ENST00000378187 CC (C19orf69-201) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000017323 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X19WM01EDTD5, F0BWDJC01B73FU, F1HESOY02F164M, F1IUBWX02I68XI, CC F1RHTUQ01B0K89 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874264|FR874264.1:130111825-130111865. XX FH Key Location/Qualifiers FH FT source 1..346 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 346 BP; 112 A; 46 C; 86 G; 102 T; 0 other; aggtgatgtg ggaggttgta gagaagttgc aggtgctttt gcagatgagc ctcagctgtg 60 ctgctgagat ggggtctctg caaatgtgat ggtgatacat acagatggga tttcagatca 120 acgctgtcca gtatggtaga cggacagcgg acgcacatgg ctatttagtg cttgaaacat 180 ggggcgtcca aattgagatg tgctgtgagt gtaaaataca caacaaatat taaaggctta 240 gtatgatgaa aataacataa gacatctcaa tcatgtttag attaattact tgttgaaata 300 ataatatttt gatcatatca gtagtagtaa aaatattact aaaatt 346 // ID JK123849; SV 1; linear; mRNA; EST; MAM; 216 BP. XX AC JK123849; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF19FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-216 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 699aa8dc29047f0e09a64d9e02d114cd. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1HESOY02HHY9X, F0M2C4T02GAEPP, F0BWDJC01D6VRL, F0BOF8H01D5LS2, CC F0DTF6N01A812X CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:130683398-130683476. XX FH Key Location/Qualifiers FH FT source 1..216 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 216 BP; 60 A; 53 C; 59 G; 44 T; 0 other; cccagaagtt tgagaccagc ctgggctaca tggcaagacc ctgtctctgc aaaatattag 60 aaaattagct gagtgtagtg gtgtgcctgt ggtcccatct acatgggagg ccagcgcagg 120 aggattgctt gagagcagga ggccaggact acagtgagcc atgtttacat cagtgcactc 180 cagcctaggc aacagaacaa gactctgtcc ccaaaa 216 // ID JK123850; SV 1; linear; mRNA; EST; MAM; 404 BP. XX AC JK123850; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF20FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-404 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; cbc839a26c4a91dc8758875177ca7c69. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000021100 (TSTD2) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000001384 CC High quality match with Homo sapiens transcript: ENST00000375172 CC (TSTD2-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000017200 CC High quality match with Microcebus murinus transcript: CC ENSMICT00000012667 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000021556 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000056428 CC High quality match with Pongo abelii transcript: ENSPPYT00000022657 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T02IHZ0L, F0DTF6N01DKJLY, F0DRJ6O01BET2X, F0BOF8H01B533W, CC F0BOF8H01CBSIG CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874258|FR874258.1:38611305-38611382. XX FH Key Location/Qualifiers FH FT source 1..404 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 404 BP; 103 A; 87 C; 108 G; 106 T; 0 other; aagagtgcct tccacatagt catgaagtgt ctgcttggct ccctgatata agctgcttta 60 accctgatga gctgataagt ggccagggca atgaagaagg ggaggtgctc ctttattact 120 gctaccgtga cctggaggat ccccagtgga tctgtgcctg gcagacagct ctgtgtcagc 180 acctgcacct cacaggcaag attcgaattg ctacagaagg aatcaatggg acagttggtg 240 gaagcagatt ggctaccaga ctctatgtgg aagtcatgct ttccttccca ttgtttaagg 300 atgacctgtg taaagatgat tttaagacca gcaaaggagg agctcactgt tttccagaat 360 tgcgtgttgg tgtatttgaa gaaatcatgc ccatggggat cacc 404 // ID JK123851; SV 1; linear; mRNA; EST; MAM; 539 BP. XX AC JK123851; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF21FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-539 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; e28bdfa1ea0b7056860b0df880824840. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig383 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000039703 CC High quality match with Homo sapiens transcript: ENST00000335295 CC (HBB-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000006878 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000006177 (HBB_PANTR) CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE02JXKN0, FZ8A1BE02IQM7U, F0M2C4T01BCZEQ, F1IUBWX01EIKD3, CC F1HESOY02FXE10 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:68763681-68763944. XX FH Key Location/Qualifiers FH FT source 1..539 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 539 BP; 119 A; 134 C; 142 G; 144 T; 0 other; gtgcatctga ctcctgagga gaagactgcc gttaccaccc tgtggggcaa ggctgctggt 60 ggtctaccct tggacccaga ggttctttga gtcctttggg gatctgtcct ctcctgatgc 120 tgttatgggc aaccctaagg tgaaggctca tggcaagaaa gtgcttggtg cctttagtga 180 tggcctggct cacctggaca acctcaaggg cacctttgcc cagctgagtg agctgcactg 240 tgacaagctg catgtggatc ctgagaactt caagctcctg ggcaacgtgc tggtgtgtgt 300 gctggcccat cactttggca aagaattcac cccgcaagtg caggctgcct atcagaaagt 360 ggtggctggt gtggctaatg ccctggccca caagtaccac taagttcact ttcttgctgt 420 ccaatttcta ccaaaggttc ctttgttccc aaagtccaac tactgaactg ggggatatta 480 tgaagggcct tgaggatctg gattctgcct aataaaaaac atttattttc attgcaaaa 539 // ID JK123852; SV 1; linear; mRNA; EST; MAM; 670 BP. XX AC JK123852; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF22FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-670 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 3e12dc7727b8fbe2e782fb79adcde853. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Homo sapiens transcript: ENST00000540098 CC (MPP7-203) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000021698 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000048766 (XM_001160943.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X19WM01D92L2, F0DTF6N01BT2XZ, F1RTSFR02I275V, F0M2C4T02JQH18, CC F0DRJ6O02JARYE CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874252|FR874252.1:133841733-133841679. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 670 BP; 207 A; 126 C; 128 G; 209 T; 0 other; taataagatg ttataaaaat ataacatttt aaaaaagaaa ggtccagacc tttgtttatt 60 taagggcaga gctagaacaa tattttttaa ataatggggg gaaaaagaag gccctttggc 120 aattttagaa atcaggtagt atactttttt tttttttttt tttgagacag ggtcttgcta 180 tgttgctcag gctggtcttg aactcctggg cttaagcagt cctcctgcct tggcctccca 240 aagtgctggg attacaggtg tgatccaccg tgcccagcct gtagacatag tcttaaaaat 300 ggtgtttaca tggccatctt gatgcttaga aagatacttg attaaaattt aataaggcag 360 ggccaactcc gagagtgcat cgataacggc agcagaaagg ccctgaattc tgaactttct 420 tcccccagcc tccttctcca gcaaggagaa tagcactcct cccttcagaa accagctccc 480 taagttggag tcactatgta agagaagagg aatgttcact ttttaaaatt catgtattta 540 aaattcaaga ccaaaaagga aattctgtac tcctattatt gactatagtc aattaaacat 600 aaaaaggtga aataaaaatc tgattttttg cccttatttt actgacccat atggaattct 660 tggtatctct 670 // ID JK123853; SV 1; linear; mRNA; EST; MAM; 613 BP. XX AC JK123853; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF23FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-613 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 8983a20beb100a7ac2f743bba534d40c. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Homo sapiens transcript: ENST00000552595 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Q2XTE01C5JLP, F0M2C4T01CUK7I, F0M2C4T01EWOB6, F1GITI001AX5QX, CC F0DRJ6O01CW7LG CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:31274233-31273879. XX FH Key Location/Qualifiers FH FT source 1..613 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 613 BP; 133 A; 179 C; 162 G; 139 T; 0 other; atgacgtctc tgaggaaatg gaggggaaga cagtccctag aatactgatc aggggtctcc 60 tttgaaccct gcagcagcct tgggcaccat gaaatcttct ctcaggcctt gttctctgcc 120 tcacactcaa tgtgtttgaa ggtctgattc cagcttttct gagtccctcg gcctccactc 180 aggtcaggac cagaagtcgc tgttccttcc tcagagccta gaactctcca aggaataaga 240 gattatccca gatgcctgtg tccaggctgg tgtctgggtt ctgtgctccc tcccccaccc 300 caggtgtcct gttcattctc aggatggtca catgcgtgct gctggggtgt cccatgaaag 360 atgcaaagcc cctgaatttt cagactcttc ccatcagatc ccccaaagac acacgtgacc 420 caccacccca tctctgacca tgaggccacc ctgaggtgct gggccctggg cttctaccct 480 gcggagatca cactgacctg gcagcgggat ggggaggatc aaactcagga caccgaactt 540 gtggagacca ggccagcagg agatggaacc taccagaagt ggggagctgt ggtggtgcct 600 tctggagaag agc 613 // ID JK123854; SV 1; linear; mRNA; EST; MAM; 445 BP. XX AC JK123854; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YF24FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-445 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 54b0a2fdf9133122e67cb870cc957f71. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig784 CC High quality match with Homo sapiens transcript: ENST00000378292 CC (TPM2-001) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z1IVZ01EWO3S, F0Z8TLB01B407X CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874258|FR874258.1:41854870-41855301. XX FH Key Location/Qualifiers FH FT source 1..445 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 445 BP; 91 A; 139 C; 147 G; 68 T; 0 other; gccagatgag actccgctac taccaccacg tcgataatgc aaacttagag ggactctggg 60 taaactgagg cactcaaatt gccgaggagc tccgcctccc aagagacatt taatccgggg 120 ggatttgcag gaaacttcta aattaagggt agcggctgct gcagctgagg gggggcacgc 180 ccgtcgctgc gcccgggcag ctgccgtgag ctcacgcccc gaaatagccc caggggcccc 240 agccgcagct gccactgggt ccggctgtca ctcagaggaa gcacggagcc cccagcccaa 300 gggtcccctc ccctccgcag cgccggagtt tttccagccg accgtctgct actttttcct 360 ccgacggctg gcggggaaga ggggaatggg ggcgggaacc caaggcccaa gggaggcggt 420 ccccagtggg tgggcgaagg gggca 445 // ID JK123855; SV 1; linear; mRNA; EST; MAM; 503 BP. XX AC JK123855; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG02FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-503 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1e97fbb9d3dd973867053297cfcc73de. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3648 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H01AP41G, F1IUBWX02JWKBY, F0BOF8H02IZUQA, F0BOF8H01EAUE5, CC FZ8FYPF02F3AKQ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:116885966-116886291. XX FH Key Location/Qualifiers FH FT source 1..503 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 503 BP; 102 A; 150 C; 171 G; 80 T; 0 other; ggcgagatgg cagaggtaga gcagaagaag aagcggacct tccgcaagtt cacctaccgc 60 ggcgtggacc tggaccagct gctggacatg tcctacgagc agctgatgca actgtacagt 120 gcgcgccagc ggcggcggct gaaccggggc ctgcggcgga agcagcactc actgctgaag 180 cgcctgcgca aagccaagaa ggaggcgccg cccatggaga agccagaagt ggtgaagacg 240 cacttgcggg acatgatcat cctgccagag atggtgggca gcatggtggg cgtctacaac 300 ggcaagacct tcaaccaggt ggagatcaag gtgcgtgcag ctgtccctgc cggccgtccc 360 tgccaaccgg cctgggcccg gggtgggctg ggttcgcttg acgcgggcag gatcgcctga 420 cacccagctc tgctgttggt ctcctgcagc ccgagatgat tggtcactac ctgggcgagt 480 tctccatcac ctaccagccc gtg 503 // ID JK123856; SV 1; linear; mRNA; EST; MAM; 222 BP. XX AC JK123856; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG03FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-222 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; c46d43afc3d9727e9b987a556bb01532. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1830 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000055815 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000009591 CC High quality match with Homo sapiens transcript: ENST00000460556 CC (GAPDH-009) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000008204 (Q6IUG4_MACMU) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000005291 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000067000 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z1IVZ01D93YA, FZ8FYPF01ELSIN, F1RHTUQ02HRA3H, F0X19WM01D777I, CC F1HESOY02FTBTU CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:60209271-60209495. XX FH Key Location/Qualifiers FH FT source 1..222 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 222 BP; 53 A; 79 C; 56 G; 34 T; 0 other; ggcccacatg gcctccaagg agtaagaccc ctggaccacc agccccagcg agagcacaag 60 aggaagagag aggccctcac tgctggggag tccctgccac actcagtccc ccgccacact 120 gaatctcccc tcacagtttc catgtagacc ccttgaagag gggaggggcc tagggagccc 180 caccttgtcg tgtaccatca ataaaatccc ctgtgctcag cc 222 // ID JK123857; SV 1; linear; mRNA; EST; MAM; 597 BP. XX AC JK123857; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG04FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-597 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 972b8f7bf7200da5fea0417238c942ea. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1305 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000001857 (LOC100401891) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000011356 CC High quality match with Homo sapiens transcript: ENST00000373493 CC (RBBP4-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000013878 (XM_001090071.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000003708 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000000953 (XR_020033.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB02GD02S, F01W12W01CQQRJ, F0BOF8H01EN2PM, F0DRJ6O01CVQS2, CC F0BOF8H01EJPEX CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874246|FR874246.1:115343942-115343346. XX FH Key Location/Qualifiers FH FT source 1..597 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 597 BP; 157 A; 99 C; 107 G; 234 T; 0 other; attatgtgtg attatttttc ttcttatgct atatatcccc aagtttttca gactcattta 60 agtaaaagct agagtgagta aggaatagag ccaaatgagg taggtgtctg agccatgaag 120 tataaatact gaaagatgtc acttttattc aggaaatagg ggagattcaa gtcatataga 180 ttcctactcg aaaatcttga cacctgactt tccaggatgc acattttcat atgtagacca 240 gtttcctctt ggtttcctca gttaagtcaa aactacatgt tcctctttcc ccatatattc 300 atatattttt gctcgttagt gtatttcttc agctgttttc atgttgtttc tttcctgtct 360 gtgaaatggt gtgttttttg ttgttttttt ttttttttaa cttgggacca ccaagttgta 420 aagatgtatg tttttacctg acagttacac cacaggcaga ctgtcaagtt gagaagagtg 480 aatcaataac ttgtatttgt tttaaaaatt aaattaatcc ttgataagag ttgctttttt 540 ttaggagtta gtccttgacc cactagtttg atgccatctc cattttgcgt gacctgt 597 // ID JK123858; SV 1; linear; mRNA; EST; MAM; 238 BP. XX AC JK123858; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG05FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-238 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 54a84880d25cd6d3e3c3082381f326eb. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig981 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000003219 (LOC100385427) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000009900 (D8KYF5_9PRIM) CC High quality match with Homo sapiens transcript: ENST00000553193 CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000027050 (2DRA_MACMU) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000033300 (XM_518384.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H02F0N6F, F0BOF8H01A6VJK, F01W12W01C2TKJ, F0DTF6N02GEFDM, CC F1IUBWX01CGC2G CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:32252007-32252232. XX FH Key Location/Qualifiers FH FT source 1..238 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 238 BP; 56 A; 53 C; 40 G; 89 T; 0 other; aacatctagc tggcttccct gtctattgcc ttttctgcta tctattttcc tctatttccc 60 acattttatt atcaccatgc aacgcctctg gaataaaaca tactggagac tgtctcggct 120 atggaacttt ctgaacatcc cacgggggca tctcttgtgt actttttgtt taaggtttcc 180 tcaaactgtg atttttttct gaacacaata aatgattttg atgatcttgg gtggaaaa 238 // ID JK123859; SV 1; linear; mRNA; EST; MAM; 541 BP. XX AC JK123859; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG07FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-541 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 35f58af6f668f475f3e73e0a2b92c861. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2676 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000019505 (LOC100411672) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000007711 CC High quality match with Homo sapiens transcript: ENST00000272317 CC (RPS27A-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000021829 (LOC705140) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000023897 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000022207 (UBIQ_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000029350 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000000842 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RHTUQ01BWVW3, F0DRJ6O02FHHOD, F0BOF8H02I25FS, F0BWDJC01DGDHB, CC F0BWDJC02IGMOT CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874251|FR874251.1:77739108-77738562. XX FH Key Location/Qualifiers FH FT source 1..541 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 541 BP; 180 A; 104 C; 130 G; 127 T; 0 other; gccatctgcg gtggagccgc caccaaaatg cagattttcg tgaaaaccct tacggggaag 60 accatcaccc tcgaggttga accctcggat acgatagaaa atgtaaaggc caagatccag 120 gataaggaag gaattcctcc tgatcagcaa agactgatct ttgctggcaa gcaactggaa 180 gatggacgta ctttgtctga ctacaatatt caaaaggagt ctactcttca tcttgtgttg 240 agacttcgtg gtggtgctaa gaaaaggaag aagaagtctt acaccactcc caagaagaat 300 aagcacaaga gaaagaaggt taagctggct gtcctgaaat attataaggt ggatgagaat 360 ggcaaaatta gtcgccttcg tcgagagtgc ccatctgatg aatgtggtgc tggggtgttt 420 atggcaagcc actttgacag acattattgt ggcaaatgtt gtctgactta ctgcttcaac 480 aagccagaag acaagtaact gtatgagtta ataaaagaca cgaactatat aaaaaaaaac 540 a 541 // ID JK123860; SV 1; linear; mRNA; EST; MAM; 636 BP. XX AC JK123860; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG08FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-636 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f801fb3164798e746a0d0a6ab1aa7662. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2044 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000017202 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000025682 CC High quality match with Homo sapiens transcript: ENST00000551150 CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000045510 (Q9GMG8_MACMU) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000012550 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000000394 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000003846 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000010142 (XR_020374.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000005944 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000009733 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1GITI001A6J6P, F0ZWYBK02JU0AD, F0M2C4T02GUYJL, F0ZWYBK02J4EMX, CC F0BOF8H02HBL89 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:12557776-12557140. XX FH Key Location/Qualifiers FH FT source 1..636 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 636 BP; 159 A; 172 C; 170 G; 135 T; 0 other; ctgacgcacc gccgtgatgc ccagggaaga cagggcgacc tggaagtcca actacttcct 60 taagatcatc caactattgg atgattatcc gaaatgtttc atcgtgggag cagacaatgt 120 gggctccaag cagatgcagc agatccgcat gtcccttcga gggaaggccg tggtgctgat 180 gggcaagaac accatgatgc gcaaggccat ccgagggcac ctggaaaaca acccagctct 240 ggagaaactg ctgcctcata tccggggaaa tgtgggcttt gtgttcacca aggaggacct 300 cactgagatc agggacatgt tgctggccaa taaggtgcca gctgctgccc gtgctggtgc 360 cattgctcca tgtgaagtca ctgtgccagc ccagaacact ggacttgggc ccgagaagac 420 ctcctttttc caggctttag gtatcaccac taaaatctcc agggcaccat tgaaatcctg 480 agtgatgtgc agctgatcaa gactggagac aaagtgggag ccagcgaagc cacgctgctg 540 aacatgctca acatctcccc cttctccttt gggctggtca tccagcaggt gtttgacaat 600 ggcagcatct acaatcctga agtgcttgac atcaca 636 // ID JK123861; SV 1; linear; mRNA; EST; MAM; 287 BP. XX AC JK123861; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG09FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-287 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 4ddb20cbd01b6490e7bb07c439bbfea7. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1575 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000053440 (STARD7) CC High quality match with Homo sapiens transcript: ENST00000337288 CC (STARD7-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000032720 (XM_001097200.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000000023 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000022705 (XM_515628.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0DRJ6O01B70ER, F01W12W02H79DQ, F01W12W02HFHUA, F1IUBWX02IL4RK, CC F01W12W01B2L7E CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874256|FR874256.1:97196593-97196307. XX FH Key Location/Qualifiers FH FT source 1..287 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 287 BP; 72 A; 48 C; 78 G; 89 T; 0 other; ctagccaggg cagagtgcct gggttgtgtc caaacttagc acctgcaatt gttgggatgt 60 ggatggatgt gtgcgtgaga gagagagaat gtgtgtgtgt gtccatccgt gtgtgtgcac 120 acatgcacag gtgcaaagga gttgcggttg ctccatgttc tgacttacgg caatttgatt 180 ctgcacttgg ggtctgtaca gttactcatg tcattgtaat gatttcactc ctaactgtga 240 catttttatc aaatgtgtga ataaataata cataaagatt ggtaaaa 287 // ID JK123862; SV 1; linear; mRNA; EST; MAM; 412 BP. XX AC JK123862; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG10FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-412 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 3fd0792742ec8b12f35be2c774f62a2f. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H01BCRV1, F0Z8TLB01DMUP5, F0M2C4T01CJTCS, F0OTZ2I02IGE89, CC FZOOKC302FSL89 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:134009433-134009306. XX FH Key Location/Qualifiers FH FT source 1..412 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 412 BP; 88 A; 96 C; 144 G; 63 T; 21 other; caggctggcc gggcgcggtg gctcaagtct gtagtcccag cacttaggga ggccgagacg 60 ggcggatcac gaggtcagga gatcgagacc atcctggcta agacggtgaa accccgtctc 120 tactaaaaat acaaaaaaaa aaaaaactag ccgggcgggg tggcgggcgc ctgtggtccc 180 ggctactctg gaggctgagg caggagaatg gcgtgaaccc gggaggcgga gcttgcagtg 240 agctgagatc tggccactgc actccagcca gggctacaga gcaagacact gtctcnnnnn 300 nnnnnnnnnn nnnnnnggcc aggcttggtg gtgtgtgcct gtggtcccgg ctgttcggga 360 ggctgaggca ggagagtcgc ttggacccag gaggaggttg cagtgagcag aa 412 // ID JK123863; SV 1; linear; mRNA; EST; MAM; 628 BP. XX AC JK123863; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG11FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-628 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 4bef63821ed4833437e50e0575e0049f. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1766 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000007327 (LOC100392267) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000031737 CC High quality match with Homo sapiens transcript: ENST00000307839 CC (RPL15-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000008915 (XR_009655.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000000111 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000004551 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000013058 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000027487 (XR_024263.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000016357 CC (RL15_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BWDJC01BXOO2, F0BOF8H02GZFL1, FZOOKC302JOKG7, F0M2C4T01AZLUB, CC FZ8A1BE02GAOD6 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874260|FR874260.1:80732840-80732216. XX FH Key Location/Qualifiers FH FT source 1..628 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 628 BP; 152 A; 168 C; 170 G; 138 T; 0 other; ggcggcagcc atcaggtaag ccaagatggg tgcatacaag tacatccagg agttatggag 60 aaagaagcag tctgatgtca tgcgctttct tctgagggtc cgctgctggc agtaccgcca 120 gctctctgct ctccacaggg ctccccgccc cacccggcct gataaagcgc gccgactggg 180 ctacaaggcc aagcaaggtt acgttatata taggattcgt gttcgccgtg gtggccgaaa 240 acgcccagtt cctaagggtg caacttacgg caagcctgtc catcatggtg ttaaccagct 300 aaagtttgct cggagccttc agtccgttgc agaggagcga gctggacgcc actgtggggc 360 tctgagagtc ctgaattctt actgggttgg agaagattcc acatacaaat tttttgaggt 420 tatcctcatt gatccattcc ataaagctat cagaagaaat cctgacaccc aatggatcac 480 caaaccagtc cacaagcaca gggagatgcg tgggctgaca tctgcaggcc gaaagagccg 540 tggccttgga aagggccata agttccacca cactattggt ggttctcgcc gggcagcttt 600 ggagaaggcg caatactctc cagcttcc 628 // ID JK123864; SV 1; linear; mRNA; EST; MAM; 666 BP. XX AC JK123864; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG12FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-666 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 73ab4463a171325b13dd9c22529708bf. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000028867 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000011299 CC High quality match with Homo sapiens transcript: ENST00000299977 CC (SLFN5-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000014077 (XM_001113957.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000017729 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000016615 (XM_523607.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000009570 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000012955 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0SMK5U02JHQRX, FZOOKC302IJWS5, F0SMK5U02GORYK, F0BOF8H01CSHB1, CC F1IUBWX01CRSWV CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874259|FR874259.1:30778224-30777717. XX FH Key Location/Qualifiers FH FT source 1..666 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 666 BP; 194 A; 158 C; 154 G; 160 T; 0 other; gcaagaccaa agagttgttt gttcatggct tacctggatc agggaagacc atcttggctc 60 ttaagatcat ggagaagatc aagaatgtgt ttcactgtga accgactaac attctctaca 120 tctgtgaaaa ttaccccctg aagaagctgg tgagcaagaa aaacatctgc cagccagtga 180 cccggaaaac cttcatgaaa aacaactttg aacacgtcca gcacattgtc attgatgatg 240 ctcagaattt ccgcactgaa gatggggact ggtatgggaa agcaaagttc atcactcaga 300 cagcaaggga tggcccagga gttctctgga tctttctgga ctactttcag accaatcact 360 tgagttgcag tggcctccca cctctctcag accagtatcc aagagaagag atcaccagag 420 tggtccgcag tgcagatcca atagccaatt acctacaaca aataatgcag gaagccagac 480 aaaatcttcc acctaacctc ccccctgggt gcctggagat gctctatgaa cctcaatggg 540 ctcaaggtgt cccaggcaac ttagagatta ttgaagactt gaacttggag gagatactgg 600 tctatgtagc ggataaatgc cgtttcctct tgcggaatgg ttattctccg agggatattg 660 ctgtgc 666 // ID JK123865; SV 1; linear; mRNA; EST; MAM; 616 BP. XX AC JK123865; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG13FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-616 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 94b5929c9a0a415fc69adbabf01be210. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000011863 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000003493 CC High quality match with Homo sapiens transcript: ENST00000453372 CC (ZNF562-203) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000028782 (XM_001098502.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000021280 (XM_001161581.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000011109 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01DEE97, F01W12W02G4197, F0Z8TLB01D86PZ, F0Z8TLB02I58PG, CC F0DRJ6O01AW39K CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874249|FR874249.1:52749384-52749757. XX FH Key Location/Qualifiers FH FT source 1..616 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 616 BP; 189 A; 117 C; 147 G; 163 T; 0 other; tgtcccttgg gtttttttcc agagaaccag tctgcccttt tgaagaaaag acgaaggtag 60 aaaggatggt ggaggactac ctggcaaatt cttatcagga ttcagtgacg tttgatgatg 120 tggctgtgga gttcacccca gaggagtggg ttttactgga cacaactcag aaatacctct 180 acagagatgt gatgctggag aactacatga acctggcctc tgtggatttc tttttctgct 240 taacttcaga atgggaaata caacctagaa ccaaacggtc atcacttcag cagggttttt 300 tgaagaatca aatattcact ggcatacaaa tgcagacaag aaactacagt ggatggaaac 360 tctgtgagaa ttgtggagag gtcttcagtg aacagttttg ccttaagaca cacatgagag 420 ctcagaatgg agggaacact tctgagggta attgttacgg aaaagacgtc ctcagtgtgc 480 acaaggaagc ctctattgga caggaacttt ccaaatttaa tccatgtgga aaagttttca 540 ccttaactcc aggccttgct gtacatcttg aaattctcaa tggaagacaa ccctacaaat 600 gtaaggaatg tggaaa 616 // ID JK123866; SV 1; linear; mRNA; EST; MAM; 633 BP. XX AC JK123866; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG14FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-633 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1e28ba075848683d561ec0365b0405e7. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RHTUQ02F0JSX, F0OTZ2I01A9PD4, F1RHTUQ02INZQ9, F01W12W02HX94V, CC F0DRJ6O01A81YC CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874255|FR874255.1:104318843-104318757. XX FH Key Location/Qualifiers FH FT source 1..633 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 633 BP; 144 A; 173 C; 215 G; 101 T; 0 other; ccagggcccc caagccagct ccccatgagc cacagcgccc ctgagccccc gctcaagttc 60 gtgcagggca aagatgagat aactgacaca ggactgagga aggagcctgg gatccccaga 120 acctgggctt cccagaaccc cagacacctg ggaccacggc gactggagtt gggggaccag 180 gagagggtgg tgagaggtga ggggacgagt atgacggtgt ggacagggtg gagatgagct 240 gtgggcacgc agtggaccca gagaacctga agctctggtg cctccagctg ataaaacagg 300 gcaagtacac actccactgc cccgcggaag tcaaggggaa gaagtgtggg gcccagtggc 360 tctacccgga ggtacgccgc tgcacccagc tgagcgactc tgagcagcag gagttcgagc 420 aagggcttgc ccaggctgca atgagacgct actgcaacct gaaagtgtgc ccaggctgca 480 ggagcctcgt ggagcggaag gatccagccg agcttcgggt gcattgcaca gtctgttgca 540 ccatacgggg tgtgccctac gacttctgct ggcagtgcat gcaggcctgg aaagggccta 600 tgttgtcttc gaactgttgt ggcaatgagg act 633 // ID JK123867; SV 1; linear; mRNA; EST; MAM; 415 BP. XX AC JK123867; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG16FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-415 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 19aaaafd64bac8d79fa809d12a1adc69. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000035794 (SPRYD3) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000028972 CC High quality match with Homo sapiens transcript: ENST00000301463 CC (SPRYD3-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000000483 CC High quality match with Pongo abelii transcript: ENSPPYT00000005411 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0DTF6N02IJA3P, F0Z1IVZ02HK63B CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:50340312-50339899. XX FH Key Location/Qualifiers FH FT source 1..415 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 415 BP; 70 A; 139 C; 109 G; 97 T; 0 other; tgaaccagtt gctgctgtca tacccctggc agggccaggg gacctctctt tggtcatctc 60 tgtcctttca ctagctgctg ccccaggaag actcctctag gctctccatt ctttcccttg 120 agagctggct ctccacccca gcctgctcag gcaccacaga gaatctgcat ctctggctcc 180 ccatacctgg acccacatgg gtgggtgcct gttgcatgtt taagagagag gggctgtgag 240 gtgacagggc actaggacct tcgctccttt tccccttcca tcctttcttt accagtgcca 300 cccatgtccc taactcccag gtactggggc tgaggctctg gggcctgtct ccctgtcagc 360 gtgagggcaa caccccagag cctcagccga gcaaggccag aggggcagcg tggcc 415 // ID JK123868; SV 1; linear; mRNA; EST; MAM; 598 BP. XX AC JK123868; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG18FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-598 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 407e61618107d83a5e86a6515fd6cd8b. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig65 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000025201 CC High quality match with Homo sapiens transcript: ENST00000322297 CC (OAZ1-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000025219 (NP_001128372.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000015472 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000002841 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000018791 CC High quality match with Pongo abelii transcript: ENSPPYT00000010900 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02HVV44, F0ZWYBK02HAHIR, F0OTZ2I02JGCS0, F0BWDJC02JAF13, CC F0OTZ2I02GI2HT CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:200244221-200244841. XX FH Key Location/Qualifiers FH FT source 1..598 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 598 BP; 132 A; 184 C; 177 G; 105 T; 0 other; gcgagcagcg gcggcggcgc ggagaggcgc agcggaggtt ttcctggttt cggaccccag 60 cggccggatg gtgaaatcct ccctgcagcg gatcctcaat agccactgct tcgccagaga 120 gaaggaaggg gataaaccca gcgccaccat ccacgccagc cgcaccatgc cgcttctaag 180 cctgcacagc cgcggaggca gcagcagtga gagttccagg gtctccctcc gctgctgtag 240 taacccgggt ccggggcctc ggtggtgctc ctgatgcccc tcacccaccc ctgaagatcc 300 caggtgggcg agggaatagt cagagggatc acaatctttc agctaactta ttctactccg 360 atgatcggct gaatgtaaca gaggaactaa cgtccaacga caagacgagg attctcaacg 420 tccagtccag gctcacagac gccaaacgca ttaactggcg aacagtgctg agcggaggca 480 gcctctacat cgagatcccg ggcggcgcgc tgcctgaggg gagcaaggac agctttgcag 540 ttctcctgga attcgctgag gagcagctgc gagcccacca tgtcttcatt tgcttcca 598 // ID JK123869; SV 1; linear; mRNA; EST; MAM; 716 BP. XX AC JK123869; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG19FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-716 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f6b18a2929ef2f3c20825ec506b1e068. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig58 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000039345 (O62872_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000012849 (Q8WMB9_9PRIM) CC High quality match with Homo sapiens transcript: ENST00000444683 CC (CR936916.1-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000027943 (NP_001108438.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000009082 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000006432 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000016632 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000062048 (Q9MYK1_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000019075 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02HXZYZ, F0ZWYBK02F6JMQ, F0X5I7101E0GL9, F0Z8TLB01A87CQ, CC FZ8FYPF02GONMF CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:31154789-31154536. XX FH Key Location/Qualifiers FH FT source 1..716 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 716 BP; 146 A; 229 C; 222 G; 119 T; 0 other; ccgactcaga agatggcgtc atggcactag gaaccctact tctcctgctc tcggccctta 60 ccaagacctg ggcgggctcc cactccttga agtatttcca cacttccgtg tcccggcccg 120 gccgcgggga gccccgcttc atctccgtgg gctacgtgga cgacacccag ttcgtgcggt 180 tcgacagcga tgccgcgagt ccgaggatgc agccgcgggc gccgtgggtg gagcaggagg 240 ggccggagta ttgggaccag gagacacgga gcgccaggga caccgcacag actttccgag 300 tgaacctgaa taccctgcgc ggctactaca accagagcga ggccgggtct cataccctcc 360 agtggatgca tggctgcgac ctggggcccg acgggcgctt cctccgcggg tatgaacagt 420 ttgcctacga cggcaaggat tatctcaccc tgaatgagga cctgcgctcc tggtccgcgg 480 tggacacggc ggctcaaatc tccgagcaaa agtcaaatga tggctctgag gcggagcacc 540 agagagccta cctggaagac acatgcgtgg agtggctccg cagatacctg gagaatggga 600 aggagacgct gcagcgctcg gaacccccaa agacacacgt gacccaccac cccgtctctg 660 atcatgaggc caccctgagg tgctgggccc tgggcttcta ccctgcggag atcaca 716 // ID JK123870; SV 1; linear; mRNA; EST; MAM; 691 BP. XX AC JK123870; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG20FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-691 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 0c314c801d5cde3455ffa6f0025ae0c3. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3649 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000015825 (LOC100404953) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000032695 CC High quality match with Homo sapiens transcript: ENST00000477892 CC (FAM162A-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000022992 (XM_001112227.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000008018 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000000367 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000028562 (XM_526284.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000015686 CC (F162A_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T02GYXSA, F0ZWYBK02FWZDM, F0ZWYBK01BFHR3, F01W12W01CXD7A, CC F01W12W01DDUWH CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874245|FR874245.1:42499958-42500137. XX FH Key Location/Qualifiers FH FT source 1..691 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 691 BP; 199 A; 145 C; 183 G; 164 T; 0 other; ctgggtgaca ttgagctcac gagcgccgcc gtccccggcg aagttctgcg ctggtcggcg 60 gagtagtagg tggccatggg gaccctccgc ggtctgctcc tggcagcggg aagctgtttt 120 aggttatgtg aaagagatgt ttactcatct ctaaggctta ccagaagctc tgatttgaag 180 ataataaatg gattttgcat aaaaccacag gaaagtccca gagctccatc ccgcacttac 240 agccacagag tgcctttaca caaacctacg gattggcaga aaaagatgct gatatggtca 300 ggtcgcttca aaaaggaaga tgaaatccca gagactgtct cgttggagat gcttgatgct 360 gcaaagaaca agatgcgcgt gaagatctgc tatctcatga ttgccctgac agtgatagga 420 tgcattgcca tgattatcag cggcaagaag gctgcccaaa gaaacgagac tttaacacgc 480 ttgaacttag aaaagaaagc tcgtctgaga gaggaagcag ctatgaaggc caaaacagag 540 tagcagaggt atccgtgttg gctggatttt gaaaatccag gaattatgtt gcaacgagcc 600 tgtattaaaa aggatgtggt atgaggatcc atttcataaa gcatggtttg cccaaacctg 660 taccatttcc gtatttctgc tgtagaagta g 691 // ID JK123871; SV 1; linear; mRNA; EST; MAM; 678 BP. XX AC JK123871; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG21FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-678 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; b145cff3969bec81826d2aa78fbf54bd. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3291 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000029646 CC High quality match with Homo sapiens transcript: ENST00000514511 CC (HNRPDL-003) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02H9XC3, F0BWDJC01CGMK2, F0DRJ6O01CJ4MR, F0Z1IVZ02HENNL, CC F0OTZ2I01D261L CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874248|FR874248.1:47406628-47406940. XX FH Key Location/Qualifiers FH FT source 1..678 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 678 BP; 237 A; 102 C; 160 G; 179 T; 0 other; gaggattttg ttttatcaca tacaccgatg aatagccagt aaaaaaattg ttagaaagca 60 gataccatca aattggttct gggaagtgtg aaatcaaagt tgcacaaccc aaagaggtat 120 ataggcagca acagcaacaa caaaaaggtg gaagaggtgc tgcggctggt ggccgaggtg 180 gtacgagggg tcgtggccga ggtcagggcc aaaactggaa ccaaggattt aataactatt 240 atgatcaagg atatggaaat tacaatagtg cctatggtgg tgatcaaaac tatagtggct 300 atggcggata tgattatact gggtataact atgggaacta tggatatgga cagggatatg 360 cagactacag tggtcaacag agcacttatg gcaaggcatc tcgagggggt ggcaatcacc 420 aaaacaatta ccagccatac taaaggagaa cattggagaa aacaggtgtg tataagagta 480 cagggaaaca gtagaaatgt ctaatttaat ttaaagatca atagacaaac gtaaaaacaa 540 aatactatgt agcctgttct tactaaattg ttgatttttt ttaattgctt tatgagcctg 600 ttttgcctaa agtgtctata gatctttaac tttaaagtct tatctcactt tctttagtat 660 tgcagaaaaa cttaagag 678 // ID JK123872; SV 1; linear; mRNA; EST; MAM; 605 BP. XX AC JK123872; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG22FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-605 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 9497e13c8f029c5a1d909e8bb8b1de7f. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000057053 (XPO1) CC High quality match with Homo sapiens transcript: ENST00000481073 CC (XPO1-014) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000043531 (XM_001115623.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000012397 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000022264 (XM_515727.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X19WM01B8EJ8, F01W12W01CM28T, FZOOKC302I197G, F0Q2XTE02IK9R3, CC F0BWDJC02FTRFM CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874256|FR874256.1:61927778-61927178. XX FH Key Location/Qualifiers FH FT source 1..605 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 605 BP; 193 A; 95 C; 101 G; 216 T; 0 other; ggcaaattcg atttgaacat atacttgtaa ttctaatgca aaattataca atttttcctg 60 taaacaatac caatttttaa ttagggagca ttttctttct agtctatttc agtctagaag 120 aaaagataat gagtaaaaca aattgcgttg tttaaaggat tatagtgctg cattgtctga 180 agttagcacc tcttggactg aattgtttgt ctagactaca tgtattacaa agtctctttg 240 gcaagattgc agcaagatca tgtgcatatc atcccattgt aaagcgactt caaaaatatg 300 ggaacacagt tagttatttt tacacagttc tttttgtttt tgtgtgtgtg tgctgtcgct 360 tgtcgacaac agctttttgt tttcctcaat gaggagtgtt gctcatttgt gagccttcat 420 taactcgaag tgaaatggtt aaaaatattt atcctgttag aataggctgc atctttttaa 480 caactcatta aaaaacaaaa caaaacaact ctggcttttg agatgactta tactaattta 540 cattgtttac caagctgtag tgctttaaga acactactta aaaagcaaaa taaaacttgg 600 tttac 605 // ID JK123873; SV 1; linear; mRNA; EST; MAM; 749 BP. XX AC JK123873; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG23FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-749 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; bca23c9910384037918a05cc8a833442. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X19WM02H6U0E, F1GITI002F2HOW, F1IUBWX01DQ3VV, F0M2C4T02JOQ4K, CC F0BOF8H02J0XRZ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874259|FR874259.1:7942690-7942153. XX FH Key Location/Qualifiers FH FT source 1..749 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 749 BP; 163 A; 230 C; 178 G; 178 T; 0 other; agaagaaaag ccatttcatc gtggcgctca aggtcctctt caagtcccag atagagaagg 60 agggcgtgga gcatcagctg cgcagagaga tcgaaatcca ggcccacctg cagtatgggt 120 tgatgccccc tgagccgaag cccctctgct ccctgacccc agctcctcac tcagccctac 180 ctcctcactc agccctacct catccagatt gctttctctg tagctacggc tagtgagccc 240 catttagccc ttagtcctgg ccagagcctt gaatccagaa atctgcctcc agtttccctt 300 tcccactccc attcctggtg tcccctgcct gggccacgtc tgaccccagt ttgctcctat 360 taatctttcc cagctctttt atgcctttgg cctgaggcct cctctgtctc ttccctcagc 420 catcccaaca tcctgcgtct ttacaactat ttttatgacc ggaggaggat ctacttgatt 480 ctagaatatg cgccccgcgg ggagctctac aaggagctgc agaagagccg cacatttgat 540 gagcagcgaa cagccacgat catggaggag ttggcagatg ctctgatgta ctgccacggg 600 aagaaggtga ttcacagaga cataaagcca gagaatttgc tcttagggct caagggagag 660 ctgaagattg ctgactttgg ctggtctgta catgcgccct ccctgaggag gaagacaatg 720 tgtggcaccc tggactacct gcccccaga 749 // ID JK123874; SV 1; linear; mRNA; EST; MAM; 553 BP. XX AC JK123874; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YG24FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-553 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 3c83de3d5d8e5606c4894d63b13ff6e7. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1145 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000022649 (B0VX78_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000028499 CC High quality match with Homo sapiens transcript: ENST00000320868 CC (HBA1-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000000859 (HBA_MACMU) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000010223 CC High quality match with Pongo abelii transcript: ENSPPYT00000008138 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z8TLB01EFJPF, F0DRJ6O02GRH4D, F0Z8TLB01EFJPF, F0DTF6N01BZQPC, CC F0M2C4T01BK5LM CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874263|FR874263.1:133866-134105. XX FH Key Location/Qualifiers FH FT source 1..553 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 553 BP; 91 A; 205 C; 155 G; 102 T; 0 other; cagaaagaac ccaccatggt gctgtctcct gccgacaaga gcaacgtcaa ggccgcctgg 60 ggtaaggtcg gcggccacgc cggcgagtat ggtgcggagg ccctggagag gatgttcctg 120 tccttcccca ccaccaagac ctacttcccc cacttcgacc tgagccacgg ctctgcccag 180 gttaagggcc acggcaagaa ggtggccgac gcgctgaccc tcgccgtggg gcacgtggac 240 gacatgcccc acgcgctgtc cgcgctgagc gacctgcacg ctcacaagct tcgggtggac 300 ccggtcaact tcaagctcct gagccactgc ctgctggtga ctctggccgc ccacctcccc 360 gccgagttca cccctgcggt gcacgcctcc ctggacaagt tcctggcttc tgtgagcacc 420 gtgctgacct ccaaataccg ttaagctgga gcctcggtgg ccatgcttct tgccccttgg 480 gcctcccgcc aggccctcct ccccttcctg cacccgtacc ccctggtctt tgaataaagt 540 ctgagtgggc ggc 553 // ID JK123875; SV 1; linear; mRNA; EST; MAM; 577 BP. XX AC JK123875; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH01FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-577 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; cdc27a7775e7bab5366db140d128fcc8. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF01CV5DP, FZ8FYPF01EA3KD, FZ8FYPF01CI24T, F0BWDJC02HJE9V, CC F1RHTUQ02G3E2O CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874252|FR874252.1:133681184-133681237. XX FH Key Location/Qualifiers FH FT source 1..577 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 577 BP; 114 A; 195 C; 133 G; 92 T; 43 other; cgctcgccac ctcccccgtg cctgggcagc gttcgcccgg ggagtccagc aggaccctgt 60 ggagttgcgg ccatnnnnnn nnnnnnnnnn nnnnnntccc ggaccctcgg cctcccggcn 120 nnnnnnnnnn nnnnnnnnnn ccggccgcca gcgacgcggg gttcaagcaa ttctcctgcc 180 tcagcctccc cagtagctgg gattacaggc atcacgtgcc ctcccccagt gtccgtggaa 240 cacgcagaca tccgggtcaa gagctacagc ttgtactcca gggagcggta catttgtaac 300 tctggtttca agcgtaaagc cggcacgtcc agcctgactg agtgcgtgct gaacaaggcc 360 acgaacatcg ctcactggac aactcccagt ctcaaatgca ttaagcccgc agcttcatct 420 cccagctcaa acaccacagc ggccacaaca gcagctattg tcccgagctc ccggctgacg 480 ccttccacat caccttccac aggaaccaca gagataggca gtcatgagtc ctcccatggc 540 ccctctcaga caacagccaa gacctgggaa ctcacgg 577 // ID JK123876; SV 1; linear; mRNA; EST; MAM; 467 BP. XX AC JK123876; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH02FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-467 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; db8e6dc7e09e4e151549b56e7b0de32d. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000003354 CC High quality match with Homo sapiens transcript: ENST00000539356 CC (TNFAIP3-204) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000012404 (XM_001096457.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000019058 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000034436 (XM_527515.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X19WM01BXKWG, F0X5I7101BDDWR, F1GITI001BP591, F1RHTUQ01BRXKD, CC F1GITI001DVXZ5 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:126817078-126816611. XX FH Key Location/Qualifiers FH FT source 1..467 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 467 BP; 122 A; 92 C; 93 G; 160 T; 0 other; ttttaaatta tattgaaatg ctgccctaga agtataatag gaaggctaca taataataac 60 ctgttttctg gttgttgttg gggcatgagc ttgtatatac actgcttgct aaactcaacc 120 agctgccttt ttaaagggag ctctagtcct gtgtgtgtaa ttcactttat ttattttatt 180 acaaacttcg agattattta agtgaagata tttcttcagc tctggggaaa acgctgcagt 240 gtcctcttga gagaacatat ttgctttgag tcaggttgtg ggcaagttcc tgaccacagc 300 gggtaaattg gcctctttgg tacactttgc ttgcctcccc gggaaaaaag gaattgcatc 360 caaggtatat atattcatca aagtttcctg cttctcctta tgaaactcca gctatgtagt 420 aaaaaaccat cctctgtgtt ctgttaatgc ctctgagtgt cccacct 467 // ID JK123877; SV 1; linear; mRNA; EST; MAM; 672 BP. XX AC JK123877; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH03FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-672 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; b890f165b7e38eab5c6bbeebc2807132. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000007874 CC High quality match with Homo sapiens transcript: ENST00000473770 CC (PISD-005) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000044067 (XM_001111812.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000005839 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000026680 CC High quality match with Pongo abelii transcript: ENSPPYT00000013627 CC (PISD_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8FYPF01D3THW, FZ8FYPF01CYK92, FZ8FYPF01AYXLG, F0DRJ6O01B66IC, CC F1BDRJG03FTYIX CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874253|FR874253.1:75770740-75770074. XX FH Key Location/Qualifiers FH FT source 1..672 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 672 BP; 136 A; 189 C; 172 G; 175 T; 0 other; gcttcgtgac gcacaccaat agagagggcg tccctatgcg caagggcgag cacctgggcg 60 agttcaacct gggctccacc atcgtgctca tctttgaggc ccccaaggac ttcaatttcc 120 agctgaaaac aggacagaaa atccgctttg gggaggccct gggctcgctc tagagcctct 180 ttcctgatta tggctgctaa gggatctttt gcaaactgag tgagcgtctt ttagagaggg 240 aggcccatga ggccatccag gtgagggcct gcctcagggt gggtgagagt ctgaccaggt 300 aggacttgaa tgactcggct cccacctgtt ctggaggtac agacaagagg tgggtgagag 360 cccccgtcat gccctcaacc tatcctgttc cttctcccta caaataaaaa gtgcaggctg 420 gactgatctg tcacatttgg atctttttaa acactgtata gacaggagat cctgcattcc 480 cgactgaacc ttcagttggt ctcgattgtc attttttctt gctgctcctc cccatcacct 540 gggctgtttt ctgttggccc cttttgtttt ttggccttaa ccctcctgct gcacagggtg 600 aggtgcctcc ttggcacaga ctgtggatgc ctcttccccc agcagagcca cacagccttc 660 atgacaacct tt 672 // ID JK123878; SV 1; linear; mRNA; EST; MAM; 681 BP. XX AC JK123878; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH04FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-681 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f306c67c5df83881bc36ce526556c013. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE02HHGGJ, F0DRJ6O02J0QQY, F01W12W02HWYCS, F1GITI001EMG3T, CC F1GITI002J58OG CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:169722553-169722579. XX FH Key Location/Qualifiers FH FT source 1..681 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 681 BP; 217 A; 101 C; 140 G; 161 T; 62 other; attatttctc acctggatta ccagctcgat ccctaatctg ctttagttca tccttcttgc 60 tacttctagg ctaaacatca aaaacagatc tggtaggggt ggggaaatga gggggaagaa 120 acaaaaacat ctgatgatgc tgcatgctgc ttaaaatctt cagtacattg atgttttgat 180 gtagcactac ataagcatta aaaaattgtg tttttcagaa tctttaaaat acaagacagt 240 gctatgtagt gaatgaaaga attagtttga aagatatctg gagaaattgc attcataaaa 300 caattggaag tgaagcagta tcaaaacagt agggcnnnnn nnnnnnnnnn nnnnnnnnnn 360 nnnnnnnnnn nnnnnnngtg ttggaagatg cgggtgagaa aatactcctg aaaatggagc 420 tgaaagagac aaagagaaaa gatgaaagcc acagaagata aatatagggg tctaaaacca 480 gactaacagt tttagaaagt gaaaaaagtt aaaatagaaa tggaggcagt gggttattag 540 aaataacata aatgactggt atggtttgtc tgtgtcctca cctaaannnn nnnnnnnnnn 600 nnnnnngaga tggagtctct ctgtcaccag gctggactgc agtggcacaa tctcggctca 660 ctgcaacctc tgcctcccgc a 681 // ID JK123879; SV 1; linear; mRNA; EST; MAM; 602 BP. XX AC JK123879; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH06FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-602 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 13fc721dfca2aa85718da78cf493a917. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2531 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000042262 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000004671 CC High quality match with Homo sapiens transcript: ENST00000542533 CC (AC105219.4-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000038793 (XM_001087372.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000012223 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000002935 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000038231 CC High quality match with Pongo abelii transcript: ENSPPYT00000022104 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02GA6M7, F0ZWYBK02IEJZ0, F0Z8TLB02G1298, F0Z8TLB02GGEF4, CC F0Z8TLB01CFB3B CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874251|FR874251.1:147040883-147040780. XX FH Key Location/Qualifiers FH FT source 1..602 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 602 BP; 121 A; 193 C; 176 G; 112 T; 0 other; agacgatgac attaagagtg tgtttgaggc ttttggcaag atcaagtctt gcacactggc 60 ccgggacccc acaactggca agcacaaggg ctatggcttc attgagtacg agaaggccca 120 gtcgtcccaa gatgctgtgt cctccatgaa cctctttgat ctcggtggtc agtacttgcg 180 ggtgggcaag gccgtcacac cgcccatgcc cctactcaca ccggccacgc ctggaggcct 240 cccacctgcc gctgctgtgg cagctgctgc agccactgcc aagatcacag ctcaggaagc 300 agtggccgga gcagcagtgt tgggtaccct gggcacacct ggactggtgt ccccagcact 360 gaccctggcc cagcccctgg ggactttgcc ccaggctgtc atggctgccc aggcacctgg 420 agtcatcaca ggtgtgaccc cagcccgtcc tcctattccg gtcaccatcc cctcagtggg 480 agtggtgaac cccatcctgg ccagccctcc gacgctgggt ctcctggagc ccaagaagga 540 gaaggaagaa gaggagttgt ttcccgagtc atagccgcca gagatgctga gcgagcagga 600 gc 602 // ID JK123880; SV 1; linear; mRNA; EST; MAM; 307 BP. XX AC JK123880; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH07FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-307 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 7dd69f481131a8da78f65c73b7199e06. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2531 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000042262 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000004671 CC High quality match with Homo sapiens transcript: ENST00000542533 CC (AC105219.4-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000038793 (XM_001087372.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000012223 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000002935 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000006263 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000038231 CC High quality match with Pongo abelii transcript: ENSPPYT00000022104 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02GA6M7, F0ZWYBK02IEJZ0, F0Z8TLB02G1298, F0Z8TLB02GGEF4, CC F0Z8TLB01CFB3B CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874251|FR874251.1:147040701-147040509. XX FH Key Location/Qualifiers FH FT source 1..307 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 307 BP; 57 A; 105 C; 87 G; 58 T; 0 other; ccactactgg caagcacaag ggctatggct tctttgagta cgagaaggcc cagtcgtccc 60 aagatgctgt gtcctccatg aacctctttg atctcggtgg tcagtacttg cgggtgggca 120 aggccgtcac accgcccatg cccctactca caccggccac gcctggaggc ctcccacctg 180 ccgctgctgt ggcagctgct gcagccactg ccaagatcac agctcaggaa gcagtggccg 240 gagcagcagt gttgggtacc ctgggcacac ctggactggt gtccccagca ctgaccctgg 300 cccagct 307 // ID JK123881; SV 1; linear; mRNA; EST; MAM; 687 BP. XX AC JK123881; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH08FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-687 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 17d7f4e300fa7170a2022425f0b4126d. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig847 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000042479 (LOC100410230) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000009417 CC High quality match with Homo sapiens transcript: ENST00000228945 CC (ARHGDIB-001) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000005095 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000008734 (XM_001158496.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RTSFR01CPAO9, F0M2C4T01EDZ44, FZ8FYPF01DK3TU, F1HESOY01B0GAS, CC F0X5I7102JDHAQ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:15305033-15304841. XX FH Key Location/Qualifiers FH FT source 1..687 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 687 BP; 214 A; 171 C; 175 G; 127 T; 0 other; gagagacaga ggcaccccgg acagagacgt gaagcactga ataaatagat caaaatgact 60 gaaaaagccc cagagccaca tgtggaagag gatgacgatg atgagctgga caacaagctc 120 aattacaagc ctccaccgca gaagtccctg aaagagctgc aggaaatgga caaagatgat 180 gagagtctaa ctaagtacaa gaaaacgctg ctgggagatg gtcctgtggt aacagatccg 240 aaagccccca atgtcgttgt cacccggctc accctggttt gtgcgagtgc cccaggacca 300 atcaccatgg accttactgg agatctggaa gccctcaaaa aagaaaccat tgtgctaaag 360 gaaggttctg aatatagagt caaaattcac ttcaaagtga acagggatat tgtgtcaggc 420 ctgaaatatg ttcagcacac ctaccgcact ggagtgaaag tggataaagc aacatttatg 480 gttggcagct atgggcctcg gcccgaagag tatgagttcc tcactccagt tgaggaggct 540 cccaagggca tgctggcccg aggcacgtac cacaacaagt ccttcttcac cgacgatgac 600 aagcacgacc acctcagctg ggagtggaac ctgtcgatta agaaggagtg gacagaatga 660 atgcatccac ccatcccttt ccccccc 687 // ID JK123882; SV 1; linear; mRNA; EST; MAM; 652 BP. XX AC JK123882; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH09FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-652 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 22209354093bbbdbe84ede1e96e847aa. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2800 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000018767 (A6MKK2_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000033614 CC High quality match with Homo sapiens transcript: ENST00000301821 CC (RPSA-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000032328 (Q3YAI0_MACMU) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000004519 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000007154 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000011956 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000027623 (XR_020516.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000016272 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000011785 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H01A2NSM, F1HESOY01C31ZP, F1HESOY01D7EN6, F1IUBWX01CTJ8L, CC F0M2C4T01EIGD5 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:167672259-167671617. XX FH Key Location/Qualifiers FH FT source 1..652 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 652 BP; 150 A; 177 C; 165 G; 160 T; 0 other; gcgctaccta cggaggggtc catacggcat tgttctggat tcccgtcgta acttaaaggg 60 aaactttcac aatgtccgga gcccttgatg tcctgcaaat gaaggaggag gatgtcctta 120 agttccttgc agcaggaacc cacttaggtg gcaccaatct tgacttccag atggagcagt 180 acatctataa aaggaaaagt gatggcatct acatcataaa tctgaagagg acctgggaga 240 agcttctgct ggcggctcgt gccattgttg ccattgaaaa ccctgctgat gtcagtgtta 300 tatcctccag gaatactggc cagagggccg tgctgaagtt tgctgctgcc actggagcca 360 ctccaattgc tggccgcttc actcctggaa ccttcactaa ccagatccag gcagccttcc 420 gggagccacg gcttcttgtg gttactgacc ccagggctga ccaccagcct ctcacagagg 480 catcttatgt taacctacct accattgctc tgtgtaacac agattctcct ctgcgctatg 540 tggacattgc catcccatgc aacaacaagg gagctcactc agtgggtttg atgtggtgga 600 tgctggctcg ggaagttctg cgcatgcgtg gcaccatttc ccgtgaacac cc 652 // ID JK123883; SV 1; linear; mRNA; EST; MAM; 558 BP. XX AC JK123883; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH11FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-558 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 44e4b3541eb8a40e97811559b7a771f2. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3318 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H02I7SUB, F0ZWYBK02JND7N, F0M2C4T02JUFPB, F0BOF8H02ILXCG, CC F1RHTUQ01EC6CB CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874256|FR874256.1:38883254-38883715. XX FH Key Location/Qualifiers FH FT source 1..558 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 558 BP; 135 A; 157 C; 112 G; 154 T; 0 other; ctttcttcaa cctggtaggc cagggttccc cgaatatata tgaccatgaa gtcaccatag 60 aagcggatac ttacttgcct gtggatgaaa ccctgattcc tataggagaa gttgcccagg 120 tgcaaggaac tgcattcgac ctgagaaagc cagtggagct gggaaaacac ctgcaggact 180 tccatctcaa tggttttgac cacaatttct gtctgaaggg atctaaagaa aagcattttt 240 gtgcaaggtt gggtactttt cacttcctga gtctgtaagg aagaaagaaa tgacaccaac 300 tcctatcttc ctctggggct tttcctctgg ccacatcagt ggcatttacc gtgctacagg 360 tgacccaata ccacatccca tattttctca aagctcccat ggcacaggtg agctctgtag 420 aatgtatccc cctcccgctg cctgccatta cccctatgcc ccctgctcag caccccttct 480 ctcactctct gcctgttctt taaggctcat tttctgttag aagccttaac tcttcctagc 540 gtaaatcacc tcggccat 558 // ID JK123884; SV 1; linear; mRNA; EST; MAM; 587 BP. XX AC JK123884; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH12FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-587 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 588bb0677112061487f98e4f7cb7a2c4. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig314 CC High quality match with Homo sapiens transcript: ENST00000353341 CC (HNRNPD-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000019697 (XM_001089297.1) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000030176 (XM_001143722.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX01AYAY1, F0M2C4T01AOD3J, FZ8FYPF01BRJFK, F0DRJ6O02GB3P5, CC F0OTZ2I01CLILQ CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874248|FR874248.1:47485944-47486267. XX FH Key Location/Qualifiers FH FT source 1..587 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 587 BP; 179 A; 95 C; 131 G; 182 T; 0 other; cttttaagga agaagaacca gtgaagaaga taatggaaaa gaaataccac aatgttggtc 60 ttagtaaatg tgaaataaaa gtagccatgt cgaaggaaca atatcagcaa cagcaacagt 120 ggggatctag aggaggattt gcaggaagag ctcgtggaag aggtggtgac cagcagagtg 180 gttatgggaa ggtatccagg cgaggtggtc atcaaaatag ctacaaacca tactaaatta 240 ttccatttgc aacttatccc caacaggtgg tgaagcagta ttttccaatt tgaagattca 300 tttgaaggtg gctcctgcca cctgctaata gcagttcaaa ctaaattttt tgtatcaagt 360 ccctgaatgg aagtatgacg ttgggtccct ctgaagttta attctgagtt ctcattaaaa 420 gaaatttgct ttcattgttt tatttcttaa ttgctatgct tcagaatcaa tttgtgtttt 480 atgccctttc ccccagtatt gtagagcaag tcttgtgtta aaagcccagt gtgacagtgt 540 catgatgtag tagtgtctta ctggtttttt aataaatcct tttgtat 587 // ID JK123885; SV 1; linear; mRNA; EST; MAM; 350 BP. XX AC JK123885; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH13FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-350 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 5079dc4e1bf6865980bb09b242c69ecb. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2845 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000028581 CC High quality match with Homo sapiens transcript: ENST00000543978 CC (DGKZ-203) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000030187 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000006671 (XM_001162820.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RTSFR02G3DCZ, F1RHTUQ02FTFTA, F01W12W01BXK2G, F1RHTUQ02GLDZM CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874257|FR874257.1:26161836-26161597. XX FH Key Location/Qualifiers FH FT source 1..350 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 350 BP; 57 A; 126 C; 118 G; 49 T; 0 other; caggccccag ggaaagaacc ccgtgccgcg ccctaaggag ccgcccaaac ctagggctgg 60 attcaggagc tgggggcctc acctgtcccc ctgaggcccc caacggaccc ggaggctcac 120 aggggaacag gacacggctg ggttggacat gcctttgcgg ggggattctg ggtcagggcg 180 ctccctggcc gcagcagatg ccctccgagg agtggagggg ctggagagag agggaggcct 240 tcggaaagag gcttcctggg ccccctggtc ttcggccggg ccctcagccc ccgctcttgc 300 cccaccccac ctcccccggg cttcctcccg gaaactcagc gcctgctgca 350 // ID JK123886; SV 1; linear; mRNA; EST; MAM; 359 BP. XX AC JK123886; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH14FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-359 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 4059355863b6de3b4e745cbd6f6daac7. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01EO2T0, FZ8FYPF02FR31B, F1GITI002GIRVU, F0X19WM01A8MON, CC F1RTSFR02IT83V CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:88559875-88559614. XX FH Key Location/Qualifiers FH FT source 1..359 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 359 BP; 114 A; 59 C; 55 G; 131 T; 0 other; cagtctattt acactgtaca aagactgtac agtgtaaaca gtctttgtac agtctattta 60 cactgtacaa agactgttga aaagcaccct acaagttcac agtacattgt aaatagatca 120 tggaaataaa attacttatt caatattact attactgcat aacattaaca tttgaatgtc 180 tgtgacaagt accctatttg ttatgtgaca ttttaagttg taataagtat taccatttaa 240 agagtgcctc ctgtgttcca ggtgctttgt gcatatgttt tctctgatcc ttaaacctag 300 caagatatta ttagtattct cattttttaa taaagagtgc ttgagtagct tgtctaatg 359 // ID JK123887; SV 1; linear; mRNA; EST; MAM; 282 BP. XX AC JK123887; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH15FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-282 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; cd1c3a9247d1357f4178f36ef2378f3b. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2391. XX FH Key Location/Qualifiers FH FT source 1..282 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 282 BP; 77 A; 87 C; 70 G; 48 T; 0 other; acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc 60 gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac 120 gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag 180 ctttaccgca ggtatcagca ccggtggtcg tgtgctggcg gtgaacagcc agacccggac 240 gctgacgctc gaccgtgaaa tcacgctgcc atcctccggt ac 282 // ID JK123888; SV 1; linear; mRNA; EST; MAM; 675 BP. XX AC JK123888; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH16FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-675 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 5670c858732a21bd8c27231ace235a38. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig981 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000003219 (LOC100385427) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000009900 (D8KYF5_9PRIM) CC High quality match with Homo sapiens transcript: ENST00000549531 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000008527 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000008402 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000033300 (XM_518384.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000020078 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000006794 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RHTUQ01EQ8HZ, F1BDRJG03GIHPF, F0BOF8H02HC7Y0, FZ8A1BE02HZQ3B, CC F1HESOY01DNG8M CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:32250368-32250651. XX FH Key Location/Qualifiers FH FT source 1..675 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 675 BP; 184 A; 172 C; 163 G; 156 T; 0 other; tccagagcac ccaagaagaa aatggccgta agtggagtcc ccgtgctagg atttttcatc 60 atagctgtgc tgatgagcgc tcaggaatca tgggctatca aagaagaaca cgtgatcatc 120 caggctgagt tctatctgaa ccctgaccaa tcaggagagt ttatgtttga ctttgatggt 180 gatgagattt tccacgtgga tatggcaaag aaggagacgg tctggcggct tgaagaattt 240 ggacgatttg ccagctttga ggctcaaggt gcattggcca acatagctgt ggacaaagcc 300 aacctggaaa tcatgacaaa gcgctccaac aataccccaa tcaccaacgt acctccagag 360 gtaactgtgc tcacaaacag ccctgtggaa ctgggagagc ccaacgtcct catctgtttc 420 atcgacaagt tctctccacc agtggtcaaa gttacatggc ttcaaaatgg aaaacctgtc 480 accacaggag tgtcagagac agtcttcctg cccagggaag accacctttt ccgcaagttc 540 cactatctcc ccttcgtgcc ctcaactgaa gacttttatg actgcaaggt ggagcactgg 600 ggcttggatg cacctcttct caagcactgg gagtttgatg caccaagccc tcttccagag 660 actacagaga acgtg 675 // ID JK123889; SV 1; linear; mRNA; EST; MAM; 640 BP. XX AC JK123889; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH17FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-640 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 903deb04aaaa088f41903eacafaecd66. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig2458 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000025322 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000033635 CC High quality match with Homo sapiens transcript: ENST00000453809 CC (BX927178.3-003) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000042082 (NP_001108436.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000007708 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000011714 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000067873 (Q9MXL5_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000020040 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1IUBWX01CK0RK, F0M2C4T02GJSOJ, FZ8FYPF02GONMF, F1HESOY02IVEIX, CC F0Z8TLB02F92I1 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:31155386-31155303. XX FH Key Location/Qualifiers FH FT source 1..640 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 640 BP; 121 A; 215 C; 218 G; 86 T; 0 other; cagaccccga gatgcgagtc atggcgcccc gaaccctcct cctgctgctc tcgggggccc 60 tggccctgac cgagacctgg gccggctcgc actccttgag gtatttccac accgccgtgt 120 cccggccagg ccgcggggag ccccgcttca tcgccgtggg ctacgtggac gacacgcagt 180 tcgtgcggtt cgacagcgac gccgagagtc cgagagagga gccgcaggcg cggtggatgc 240 agcaggaggg gccggagtat tgggaagagc agacacggat cgccaagggc aacgcacaga 300 ctcaccgagt ggacctgcgg accctgctcc gctactacaa ccagagcgag gccgggtctc 360 acaccctcca gacgatgtac ggttgcgacc tgggacccga cggtggcctc ctccgcgggt 420 atcaccagtc cgcctacgat ggcaaggatt acatcgccct gaacgaggac ctgcgctcct 480 ggaccgccgc ggacatggcg gctcagaaca cccagcgcaa gtgggaggcg gcccgtgcgg 540 cggatcggca gagagcctac ctggagggcc ggtgcctgga gtggctccgc agatacctgg 600 agaacgggaa ggagactctg cagcgcgcgg atcccccaaa 640 // ID JK123890; SV 1; linear; mRNA; EST; MAM; 627 BP. XX AC JK123890; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH18FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-627 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 4ae16f6c3f5aecccffacdf1e86f54063. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3650 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000018031 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000003656 CC High quality match with Homo sapiens transcript: ENST00000532642 CC (ATP6V0B-012) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000047311 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000017746 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000001176 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H02HGPIO, F0Z8TLB02IQB6H, F0DRJ6O01E1EZ4, F0Z1IVZ01CDBZ1, CC F0SMK5U02GWB5E CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:47029507-47029673. XX FH Key Location/Qualifiers FH FT source 1..627 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 627 BP; 99 A; 183 C; 182 G; 163 T; 0 other; ggacgctggg acgcgtttgt aactccggcc ccacggttcc gaccctcgcc gccgtcgctg 60 ccatgacggg gctagcgctg ctctactccg gggtcttcgt ggccttctgg gcctgcgcgc 120 tggccgtggg aatctgctac accatttttg atttgggctt ccgctttgat gtggcatggt 180 tcctgacgga gacttcgccc ttcatgtggt ccaacctggg cattggccta gctatctccc 240 tgtctgtggt tggggcagcc tggggtatct atattactgg ctcttccatc attggtggag 300 gagtgaaggc ccccaggatc aagaccaaga acctggtcag catcatcttc tgtgaggctg 360 tggccatcta cggcatcatc atggcaattg tcattagcaa catggctgag cctttcagtg 420 ccacggaccc caaggccatt ggccatcgga actaccatgc aggctactcc atgtttgggg 480 ctggcctcac cgtaggcctg tctaacctct tctgtggagt ctgcgtgggc atcgtgggca 540 gtggggctgc cctggccgat gctcagaacc ccagcctctt tgtaaagatt ctcatcgtgg 600 agatcttttg gcagtgccat tggcctc 627 // ID JK123891; SV 1; linear; mRNA; EST; MAM; 481 BP. XX AC JK123891; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH19FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-481 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; daa0bdc9fef091dffff33e99e24baa83. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1368 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000026611 (LOC100395987) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000003892 CC High quality match with Homo sapiens transcript: ENST00000448223 CC (SMC6-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000043747 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000002224 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000004816 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000021759 (XM_001136387.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000014672 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000007473 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1GITI001CP3QM, FZ8FYPF01AMOG3, F1RTSFR01CONQE, F1RTSFR02F6WJR, CC F0DTF6N01EQ8U8 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874256|FR874256.1:17674760-17674689. XX FH Key Location/Qualifiers FH FT source 1..481 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 481 BP; 147 A; 91 C; 97 G; 146 T; 0 other; taaaaaagtt tattaaatta ttgtggagaa atcatggacc acagattcaa aacatatcaa 60 caatttagaa ggtgtttgac tttacgatgc aaattgtact ttgacaactt actatctcag 120 cgggcctatt gtggaaaaat gaattttgac cacaagaatg aaactctaag tatatcagtt 180 cagcctggag aaggaaataa agctgccttc aatgacatga gagccttgtc tggaggcgaa 240 cgttctttct ccaccgtgtg ttttattctt tccctatggt ccatcgcgga atctcctttc 300 agatgcctgg atgagtttga tgtctacatg gatatggtta ataggagaat agccatggac 360 ttgatactga agatggcaga ttcccagcgt tttagacagt ttatcttact tacacctcag 420 agcatgagtt cacttccatc gagtaaactg ataagaattc tccgaatgtc tgatcccgaa 480 a 481 // ID JK123892; SV 1; linear; mRNA; EST; MAM; 488 BP. XX AC JK123892; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH20FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-488 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; ac77d987c7e26c7ba4bb79f06070a17e. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1BDRJG03FTY4E, F0BOF8H01AYZRG, F0M2C4T02G0JAW, F1HESOY01DZKA1, CC FZ8A1BE02I8MGG CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:170744870-170744936. XX FH Key Location/Qualifiers FH FT source 1..488 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 488 BP; 113 A; 102 C; 90 G; 183 T; 0 other; agactggtct tgaactcctg atctcaggcc atccacctgc ctcggcctcc caaagtgctg 60 ggatttacag gtgtggccca ctacacccag tctttttttt taatccattt tgagttactt 120 tttgtatgtg gtgtgaggta ggggtccaaa aagtatcatt cttttggatg tggatatcca 180 gttttcccag aaccattttt tgaaaagatt acttcttctc catcgaatgg tcttggcacc 240 cttgttgaaa atcaattgtc catagatgta tgggtttact tctggactct aaattctatt 300 ccattgatgt ttatctctgt ccttatgccc ataccacaca ctcttttgat tagtgtagct 360 atgtagtaag ttttgaaact ggaagctgtg agtcttccaa ctttgttatt ttttcagtat 420 tattttagat aatcaggacc ccttgcaatt ccatatgaat ttgagaggat caacttttcc 480 atttctgc 488 // ID JK123893; SV 1; linear; mRNA; EST; MAM; 620 BP. XX AC JK123893; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH21FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-620 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; e9db69a035dd46015410a09741834a7f. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig15 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000044058 (LOC100393376) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000022944 CC High quality match with Homo sapiens transcript: ENST00000294189 CC (RPL29-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000027035 (XM_001091271.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000009037 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000028050 (XR_025384.1) CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000007133 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0OTZ2I01BX9GK, F1GITI001BZ9CC, FZOOKC302FYYVG, F0Z8TLB02IYE94, CC F0M2C4T02II8G2 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874260|FR874260.1:16348955-16349563. XX FH Key Location/Qualifiers FH FT source 1..620 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 620 BP; 170 A; 188 C; 165 G; 97 T; 0 other; aggcgcttcg ggatccgcgg ctaaaggtgc agacatggcc aagtccaaga accacaccac 60 acacaaccag tcccgaaaat ggcacagaaa tggtatcaag aaaccccgat cacaaagata 120 cgaatctctt aagggggtgg accccaagtt cctgaggaac atgcgctttg ccaagaagca 180 caacaagaag ggcctaaaga agatgcaggc caacaatgcc aaggccatga gtgcacgtgc 240 cgaggctgtc aaggccctcg taaagcccaa ggaggttaag cccaagatcc caaagggtgt 300 cagccgcaag cttgatcgac ttgcctacat tgcccacccc aagcttggga agcgtgctcg 360 tgctcgcatt gccaaggggc tccggctgtg ccggccaaag gccaaggcca aggccaaggc 420 caaggatcaa accaaggccc aggctgcagc tccagcttca attccagctc aggctcccaa 480 aggtgcccag gccactacaa aggctacaga gtaggtatct ctgccaacct gaggacagaa 540 ggactggtgc gaccccccac ccccacctct gggctgccat ctgcatgggg ctggggtcct 600 cctgtgctat ttgtacaata 620 // ID JK123894; SV 1; linear; mRNA; EST; MAM; 713 BP. XX AC JK123894; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH22FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-713 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 6e077b1a97ce687da2ed55805a4b64a6. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig634 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000005318 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000029055 CC High quality match with Homo sapiens transcript: ENST00000372805 CC (CAP1-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000047366 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000015449 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000059352 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1HESOY01AOP8B, FZ8FYPF02I8NFC, F0DTF6N01AXGV1, F1HESOY01AFM95, CC F0OTZ2I02IFFJ8 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874252|FR874252.1:43906291-43906158. XX FH Key Location/Qualifiers FH FT source 1..713 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 713 BP; 204 A; 160 C; 155 G; 194 T; 0 other; cttacatata caagtgtgtc aacacgacat tgcaaatcaa gggcaaaatt aactccatta 60 cagtagataa ctgtaagaaa cttggtctgg tattcgatga cgtggtgggc attgtggaga 120 taatcaacag tagggatgtc aaagttcagg taatgggtaa agtgccaacc atatccatca 180 acaaaacaga tggctgccat gcttacctga gcaagaattc cctggattgt gaaatagtca 240 gtgccaaatc ttccgagatg aatgtcctca ttcctacaga aggcggtgac tttaatgaat 300 tcccagttcc tgagcagttc aagaccctct ggaatgggca gaagttggtc accacagtga 360 cagaaattgc tggataagcg aagtgccact gggttctttg ccctcccttc acaccatgag 420 ataaatctgt atcaagacgg ttcttttcta gatttcctct acctttctgc tcttaaaact 480 gcttctctgc tctgagaagc acagctacct gccttcactg aaatatacct caggctgaaa 540 tttggggtgg gatagcaggt cagttgatct tctgcaggaa ggtgcagctt ttccatatca 600 gctcaaccac accaccagtc cattcttaag gaactgccga ctaggactga tgatgcattt 660 tagctttgag cttttggggg ttattctacc aacaaacaaa cagtccattg gaa 713 // ID JK123895; SV 1; linear; mRNA; EST; MAM; 460 BP. XX AC JK123895; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH23FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-460 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 353adbf58171499331fd917b20b9ebf1. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Homo sapiens transcript: ENST00000430195 CC (CYB561D1-002) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000058906 (XM_001148197.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1GITI001DVM08 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874244|FR874244.1:113239709-113240106. XX FH Key Location/Qualifiers FH FT source 1..460 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 460 BP; 93 A; 129 C; 90 G; 148 T; 0 other; aagctaagtc ctgattccaa atttgattat attccaaatt tgattatatt ccaagtttga 60 ttatattcag attcaacctt gaggatatta gaaatataga tggcttctgt atccaaatct 120 gaatggaaat gagattaccc ttctgggttc agatacccct cccaagcctg cgtctttcct 180 gtaaccaccc ctcctctgag ccccatcatg gctggggttg gtgtggaatg ggaggagctc 240 tgtgtctggg gaagtcctcc aacctcacct tcttggcttt taagtggtag gctgttacca 300 cttgccattc tgggcctcct ctctccccac cctttcacaa ggcaggctgc ccttcgcact 360 cactggccca tcccttcttt ctcatcgtcc cacccttcca cccccgtgca tggctgctgt 420 aggtctggtt tttttttttt taaaatggag tctcactccg 460 // ID JK123896; SV 1; linear; mRNA; EST; MAM; 622 BP. XX AC JK123896; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YH24FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-622 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; ab765c829b477a85d1736ed574e85f48. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3651 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000019684 (LOC100406649) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000028357 CC High quality match with Homo sapiens transcript: ENST00000451882 CC (HSBP1L1-201) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1HESOY01AM3L4, F0DTF6N01BNI29, FZ8FYPF01EOWPN, F1IUBWX02GZKZL, CC F1RTSFR02H5WK5 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874261|FR874261.1:73549713-73550054. XX FH Key Location/Qualifiers FH FT source 1..622 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 622 BP; 169 A; 172 C; 158 G; 123 T; 0 other; cgccacctcg cgccgggtcc gcgcggccca cgggaccccg ctgaagctcc cggccacggt 60 ccacatggac gcgcggggcc ctgaagcccc cggcgggcgc gcgctgcggg atgcggcaga 120 aaatctgttt caggaacttc aggaacattt tcaagctctg actgcaacat taaacctcag 180 aatggaagaa atgggaaacc gcatcgagga cttacagaag aatgtcagtg acttaatggt 240 gcaagctggc attgaaaatt ctattaaaca acaaatgctg aagacctaac tgcagcatga 300 ctgtgtttgg agatggggac tctacagaag tcattaaggt tacaggggtc ctgagggtgg 360 ggccctcatc caacaggatt cgtctttctg agaagacatg ccaggggctc acctgctctc 420 cccctgtccc tgccccagag aaggcggcca ttggcaagcc aacgagagcc ctccccagaa 480 atcacacgga ccggcacctt gatctgggac ttccagaaaa caactgtgtt gtttaaacca 540 cccaatctga agtcttgtta tggcagtcct gggcagacta atacaatacg caacacattt 600 ataataaata cacaataaac tt 622 // ID JK123897; SV 1; linear; mRNA; EST; MAM; 518 BP. XX AC JK123897; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI01FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-518 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 320cb2453d9bb6da32e5cbac38475d08. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig674 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000000131 (LOC100396027) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000002948 CC High quality match with Homo sapiens transcript: ENST00000391857 CC (RPL13A-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000010560 (B5MBT6_MACMU) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000009316 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000009966 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000021004 (XR_020209.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000011918 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H01DFM5L, F0ZWYBK01ARTBD, F0OTZ2I01DDF9A, F0DTF6N02FR1GN, CC F0OTZ2I02ITBE9 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874259|FR874259.1:31024077-31023559. XX FH Key Location/Qualifiers FH FT source 1..518 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 518 BP; 117 A; 147 C; 159 G; 95 T; 0 other; ggcggaggtg caggtcctgg tgctcgatgg tcgaggccat ctcctgggcc gcctggcggc 60 catcgtggct aaacaggtac tgctgggccg gaaggtggtg gtcgtacgct gcgaaggcat 120 caacatttct ggcaatttct acagaaataa gttgaagtac ctggctttcc tccgcaagcg 180 gatgaacacc aacccttccc gaggccccta ccacttccgg gcccccagcc gcatcttctg 240 gcggacggtg cgaggcatgc tgcctcacaa gaccaagcga ggccaggctg ccctggaccg 300 cctcaaggtg tttgacggca tcccaccgcc ctatgacaag aaaaagcgga tggtggttcc 360 tgctgccctc aaggttgtgc gtctgaagcc tacaagaaag tttgcctatc tggggcgcct 420 ggctcacgag gttggctgga agtaccaggc agtgacagcc accttggagg agaagaggat 480 agagaaagcc aagatccact acaggaagaa gaagcagc 518 // ID JK123898; SV 1; linear; mRNA; EST; MAM; 274 BP. XX AC JK123898; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI02FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-274 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; ebe1b1d9fdf7ad283ee6453d58e6a764. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3203 CC High quality match with Homo sapiens transcript: ENST00000356742 CC (HVCN1-202) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000018012 (XM_001107933.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000004036 CC High quality match with Pongo abelii transcript: ENSPPYT00000005869 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Q2XTE01EYVO4 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:112183895-112183621. XX FH Key Location/Qualifiers FH FT source 1..274 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 274 BP; 70 A; 75 C; 43 G; 86 T; 0 other; aaggaattga tgcatccttt catatgagca ttctgaaaga aagaaaaaga agctacttta 60 gctgccaccc cattctagaa aagtctctta ttttcaagct gttctaaata gcttcccttg 120 tctcagtttc cccaaaaggg gtacccaggt ccctcctctg tgtgccccag ctccatcagc 180 cagcttctag gtggctccat tgcgttctgc cacctgatat ttttcctcaa ttactgtaca 240 attactgtat aaaataaact ctctctttcc cctg 274 // ID JK123899; SV 1; linear; mRNA; EST; MAM; 697 BP. XX AC JK123899; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI03FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-697 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; a051fd8a78904b0050255f3625fd17cd. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3652 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000033431 CC High quality match with Homo sapiens transcript: ENST00000379935 CC (RBL2-201) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000001365 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000050640 (XM_001166080.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000008640 CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZ8A1BE02GTD6E, FZ8A1BE02JGBB3, F1HESOY01AZ3T1, F0ZWYBK02H73A0, CC F1IUBWX02JWWVD CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874263|FR874263.1:51796019-51796700. XX FH Key Location/Qualifiers FH FT source 1..697 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 697 BP; 217 A; 142 C; 132 G; 206 T; 0 other; tcagcaacag tccttcaaag agactgagag aaattaatag tatgatacgc acaggagaaa 60 ctccaactaa aaagagagga attcttttgg aagatggaag tgaatcacct gcaaaaagaa 120 tttgcccaga aaatcattct gccttattac gccgtctcca agatgtagct aatgaccgtg 180 gttcccactg aggttagtct cttgtattga aactcttttc acaacatgtt tagccgcatc 240 ctttaatgca tctagattat ggagcttttt cccttaatcc agctgatcag ttacagcctg 300 ttagtaacat gaggggatat tttggtgaga aatgggactt aactccttcc agtatcctta 360 gaacatttta attcatccca actgtcttct tttccctacc attcagtgat tactgtcaag 420 gctgcttaga atccaaactt ggatttttga ctctgacaaa gcttttaaaa gtactacaag 480 aaaatgatgt gtacccaaat gtgagcatac gaggcttctg ttgacatact ccaacagaag 540 aactgtttca agttcactcc tacctgtttt gtggtcagct gtagtcctca taaaaagcaa 600 aacaaaaatt aggtattttg tcctaaaaca cctggtagga gtgtgtgatt tttacattcc 660 tgaaacagaa caaaggagag cacacccagg tttggag 697 // ID JK123900; SV 1; linear; mRNA; EST; MAM; 621 BP. XX AC JK123900; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI04FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-621 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; c6c5bf50cbaf86739012b21c2dd16771. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC FZOOKC302HO3HF, F0M2C4T02GGBAL, F0ZWYBK01B3WSF, F1RHTUQ02HICZE, CC F1RHTUQ02FHNJ9 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874255|FR874255.1:14298206-14297913. XX FH Key Location/Qualifiers FH FT source 1..621 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 621 BP; 199 A; 141 C; 157 G; 124 T; 0 other; ccagatttag tatttgagca gtggttaagt gctaaagaaa aatcgccgtt aaagcagttt 60 tctttttcac tttttccttc ggcagggacc tgagctgttc ctgcaaggcc cacctcctca 120 ggactacccc cccaggtctc ctgaggcgct tctgcaggag gcagcgccag tttcgacagc 180 ccggcccttc ccctcccagt gcctccgggg gttccggcgt cccgggcgct gctgttttcc 240 gggaagagca ggcgcgctgg gcctcggcga gctgcgctcg gcgggcggac gcggggaatc 300 atggaagctg ataaagacaa cacacaacaa gttcttaagg agcatgggcc acatgaattt 360 ataaaagatg aacaaaataa ggaactaatt gatgaaatga caaaggaaaa tattcaacta 420 aagaaggaga tccaaaagct cgaaacggag ttacaagagg ctaccagaga attccaggga 480 atcatggaag ctgataaaga caacacacaa caagttctta aggagcatgg gccacatgaa 540 tttataaaag atgaacaaaa taaggaacta attgatgaaa tgacaaagga aaatattcaa 600 ctaaagaagg agatccaaaa g 621 // ID JK123901; SV 1; linear; mRNA; EST; MAM; 599 BP. XX AC JK123901; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI05FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-599 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 1e3dd520b3533be3b68b105a6decc616. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000034536 (B0KWM3_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000008006 CC High quality match with Homo sapiens transcript: ENST00000374273 CC (SPAG4-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000029475 (XM_001098382.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000013122 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000024987 (XM_514609.2) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0M2C4T01AOJRN, F0X19WM02GVPIM, F0Z1IVZ01DY4GO, F0M2C4T01B1EP2, CC F0BOF8H01DO8CE CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874253|FR874253.1:28912057-28911632. XX FH Key Location/Qualifiers FH FT source 1..599 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 599 BP; 97 A; 192 C; 217 G; 93 T; 0 other; ctgtgagaca tatgcaggtg cggccgcggg gcctgccgac ttcacgcagg gtccgtgtgg 60 tccccgcggc gcgcagcggc tgaaggaggt cccagggcct tggcgaccgc ggcggcggct 120 ttagcgtccg tgactgggca gcagggggtc aggatgcggc gaagctcccg cccgggctcg 180 gcctcgtcct cgcgcaagca cacgcccaac tttttcagcg agaacagctc agtgagcatc 240 acctcggagg acagcaacgg gctccggtca gcggggccgg ggcctgggga ccccgagggc 300 agaggagccc ggggcccgag ctgtggtgag cccgccttga gcgcgggagt gcccagagga 360 accacatggg caggaagctc tcggcagaag ccggcgcctc ggagccacaa ctggcagaca 420 gcctgtggcg cggcaaccaa cccactgggt ctccagcagt ctctgaggag ccgctcgacc 480 ttctgccgac cctggatctg aggcaggaga tgcctccctc gcgggtgttc aagagctttc 540 tgagccctgc tcttccaggt gctgagcgtg ttgttatccc tggcaggaga cgtgctggt 599 // ID JK123902; SV 1; linear; mRNA; EST; MAM; 643 BP. XX AC JK123902; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI06FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-643 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; b6e485d0d733799d1825d95c37995ccd. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig981 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000003219 (LOC100385427) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000009900 (D8KYF5_9PRIM) CC High quality match with Homo sapiens transcript: ENST00000553193 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000008527 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000008402 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000033300 (XM_518384.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000020078 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000006794 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1RHTUQ01EQ8HZ, F1BDRJG03GIHPF, FZ8A1BE02HZQ3B, F0BOF8H02HC7Y0, CC F1HESOY01DNG8M CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874247|FR874247.1:32250368-32250645. XX FH Key Location/Qualifiers FH FT source 1..643 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 643 BP; 173 A; 167 C; 153 G; 150 T; 0 other; cccctgagct ctactgactc cagagcaccc aagaagaaaa tggccgtaag tggagtcccc 60 gtgctaggat ttttcatcat agctgtgctg atgagcgctc aggaatcatg ggctatcaaa 120 gaagaacacg tgatcatcca ggctgagttc tatctgaacc ctgaccaatc aggagagttt 180 atgtttgact ttgatggtga tgagattttc cacgtggata tggcaaagaa ggagacggtc 240 tggcggcttg aagaatttgg acgatttgcc agctttgagg ctcaaggtgc attggccaac 300 atagctgtgg acaaagccaa cctggaaatc atgacaaagc gctccaacaa taccccaatc 360 accaacgtac ctccagaggt aactgtgctc acaaacagcc ctgtggaact gggagagccc 420 aacgtcctca tctgtttcat cgacaagttc tctccaccag tggtcaaagt tacatggctt 480 caaaatggaa aacctgtcac cacaggagtg tcagagacag tcttcctgcc cagggaagac 540 caccttttcc gcaagttcca ctatctcccc ttcgtgccct caactgaaga cttttatgac 600 tgcaaggtgg agcactgggg cttggatgca cctcttctca agc 643 // ID JK123903; SV 1; linear; mRNA; EST; MAM; 696 BP. XX AC JK123903; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI07FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-696 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; f2608938aa1d28b4e0a793a6d78941cd. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig573 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000015186 (LOC100409125) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000002985 CC High quality match with Homo sapiens transcript: ENST00000217958 CC (PSMD10-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000023864 (XM_001098141.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000002058 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000017864 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000043570 (XR_024353.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000024049 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000009920 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F1GITI001COH42, FZ8FYPF01AZ1Z1, F0Q2XTE02GER74, F0Z1IVZ02F9DXQ, CC F0BWDJC02G9H8G CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874253|FR874253.1:13508301-13507616. XX FH Key Location/Qualifiers FH FT source 1..696 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 696 BP; 205 A; 143 C; 175 G; 173 T; 0 other; ggagagtatt ctggccgata aatccctggc tactagaact gaccaggaca ggagaactgc 60 attgcactgg gcatgctcag ctggacatac agaaattgtt gaatttttgt tgcaacttgg 120 agtgccagtg aatgataaag acgatgcagg ttggtctcct cttcatattg cggcttctgc 180 tggccgggat gagattgtaa aagcccttct gggaaaaggt gctcaagtga atgctgtcaa 240 tcaaaatggc tgtactcccc tacattatgc agcttccaaa aacaggcatg agatcgctgt 300 catgttacta gaaggcgggg ctaatccaga tgctaaggac cattatgagg ctacagcaat 360 gcaccgggca gcagccaagg gtaacttgaa gatgattcat atccttctgt actacaaagc 420 atccacaaac atccaagaca ctgagggtaa cactcctcta cacttagcct gtgatgagga 480 gagagtggaa gaagcaaaac tgctggtgtc ccaaggagca agtatttaca ttgagaataa 540 agaagaaaag acacccctgc aagtggccaa aggcggcctg ggtttaatac tcaagagaat 600 ggtggaaggt taaacagctt ggatttattc ttactttgta tgttgtggtg ttgtccccag 660 tgttctgcaa actaatgtat ttgtgcacaa gacatc 696 // ID JK123904; SV 1; linear; mRNA; EST; MAM; 416 BP. XX AC JK123904; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI09FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-416 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 3521a52144f4a1ffab9ebf7844fcbf0d. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1712 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000002446 (LOC100387153) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000007251 CC High quality match with Homo sapiens transcript: ENST00000412616 CC (MLEC-004) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000000273 (XM_001090412.1) CC High quality match with Microcebus murinus transcript: CC ENSMICT00000008689 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000020216 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000010173 (XM_522542.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000005960 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Z1IVZ01ATRO3, F0BOF8H01CW6XH, F1RTSFR02I72FD, F0Q2XTE01A4G63, CC F0X19WM01CZ7N3 CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874254|FR874254.1:122304840-122304964. XX FH Key Location/Qualifiers FH FT source 1..416 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 416 BP; 66 A; 115 C; 165 G; 70 T; 0 other; agccggccga ggacgagggt cggcgggggc tgcccccgtg gtggtggccg ccatgctggg 60 agcctggacg gttgagggaa ccgctgtggc gctcttgcga ctgctgctgt tgctgctgcc 120 ggcgatccag ggaccggggc tcggcgtggc cggcttggcc ggcgtggctg gcgcggcggg 180 gaccgggctg cccgagagcg tcatttgggc ggtcaacgcg ggcggagagg cgcatgtgga 240 cgtgcacggg atccacttcc gcaaggaccc tttggaaggc cgggtgggcc gagcctcaga 300 ctatggcatg aaactgccaa tcctgcgttc caaccctgag gaccggattc tgtatcaaac 360 tgagcggtac catgaggaga cctttggcta tgaagtgccc atcaaagagg aggggg 416 // ID JK123905; SV 1; linear; mRNA; EST; MAM; 652 BP. XX AC JK123905; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI10FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-652 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 5340343b602b97053086d8aa27eaa7ca. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig944 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000034720 CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000030729 CC High quality match with Homo sapiens transcript: ENST00000323563 CC (MRPS31-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000002683 CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000001465 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000010682 (XM_509641.2) CC High quality match with Pongo abelii transcript: ENSPPYT00000006281 CC High quality match with Tarsius syricht transcript: CC ENSTSYT00000002174 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0OTZ2I01DN9R0, F0BWDJC02HFSI5, F0X19WM01CLEHC, F1IUBWX02JS439, CC F1HESOY01AO6CV CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874264|FR874264.1:85230863-85230217. XX FH Key Location/Qualifiers FH FT source 1..652 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 652 BP; 209 A; 151 C; 156 G; 136 T; 0 other; agccccctta cccgccaccc tttgtcctct ggaagcccgg agacatcagc ggctgcgatt 60 atgctactcg ctgcccggca cggaacgatc aggatgaaaa atatccaaag atattttggc 120 actaacagcg tgatctgtag caagaaagat gagcagtctg ttcgaactga tgagacttcc 180 aaggagactt cagacagcca agacagtgca aaggaaaata cgaaaaaaga cttgttagac 240 attattaagg gcatgaaagt tgaattaagc acagtaaatg tacaaacaac aaagcccccc 300 aacagaagac cacttacaag tttggaagct acacttggca ggcttcaaag agctacagaa 360 tatgctccaa agaagagaac tgagcccctg agtcctgagt tggtggcggc tgcatctgct 420 gtggcagatt ctctcccttt tgacaagcaa acaacaaagt cagagctgct gaggcagctc 480 cggcagcatg aggaagagtc aagggcacag agaggtgcag agcgacctaa aattagtttc 540 agtaacataa tatcagatat gaaagttgcc agaccttctg cagctagagt tcgttcaaga 600 ccggagcatc agattcaatt tgatgatggc tatgacagtt ctcctgacca gc 652 // ID JK123906; SV 1; linear; mRNA; EST; MAM; 567 BP. XX AC JK123906; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI12FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-567 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; dcd4d9d7c37c55f78af311b3fe54f1a2. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0ZWYBK02GDZ5W, F0BOF8H02IHX8G, F0BWDJC01AIDZB, F0M2C4T01AOFYB CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874250|FR874250.1:42124920-42124350. XX FH Key Location/Qualifiers FH FT source 1..567 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 567 BP; 117 A; 128 C; 181 G; 141 T; 0 other; aaaggaacct ggccattgtg tctctggcac tgaggttgag gggaacagag gccaggaggg 60 tagcactcta gaaagccagg gagcccctcc tgtcattgag acacacactc cggccaaacg 120 gacttctgcc ttttccttgg attccaggag cagcagtctt ggacaggggc agtggagggt 180 gaggtatggt ggggagggaa gggtgggtgg gagcagagca gtatcctccc tctggccact 240 gtggttctca gcgtacattc ttacatgggt gttttgatgt atttcagcct ctgagaatgt 300 tggcagacca ccagcttttc tcagggtcaa gctcccttga ataagtcgac atacttctcc 360 acaccatccc cactggcctg tccaccatct aggttgctgc gatggagtgg tggtgtggga 420 ttggaggagg ggtgggggat tgaggctggg gacagagcag ttgctgtgga agatttgagt 480 cctgtgtggc agcctttccc ttggagacaa gatcctgggc taggctgttg gcacatgaat 540 gacttttgga ctacaatcta ccctttt 567 // ID JK123907; SV 1; linear; mRNA; EST; MAM; 648 BP. XX AC JK123907; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI13FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-648 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 0faa986e48984f0b938d5f770cdef481. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1180 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000058826 (B0KWA9_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000023460 CC High quality match with Homo sapiens transcript: ENST00000391977 CC (EEF1A1-202) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000029930 (Q3YAI9_MACMU) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000005994 CC High quality match with Otolemur garnettii transcript: CC ENSOGAT00000009153 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000048233 (EF1A1_PANTR) CC High quality match with Pongo abelii transcript: ENSPPYT00000019520 CC (EF1A1_PONAB) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0OTZ2I01C3M18, FZ8FYPF02IRD35, F0OTZ2I02H5RVS, F0BOF8H01EKXZD, CC F1C5L3D04IM0GF CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874246|FR874246.1:182693640-182692994. XX FH Key Location/Qualifiers FH FT source 1..648 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 648 BP; 170 A; 137 C; 168 G; 173 T; 0 other; atctgtcgaa atgcaccatg aagctttgag tgaagctctt cctggggaca atgtgggctt 60 caatgtcaag aatgtgtctg tcaaggatgt tcgtcgtggc aacgttgctg gtgacagcaa 120 aaacgaccca ccaatggaag cagctggctt cactgctcag gtgattatcc tgaaccatcc 180 aggccaaata agtgctggct atgcccctgt gttggattgc cacacggctc acattgcatg 240 caagtttgct gagctgaagg aaaagattga tcgccgttct ggtaaaaaac tggaagatgg 300 ccctaaattc ttgaagtctg gtgatgctgc cattgttgat atggttcctg gcaagcctat 360 gtgtgttgag agcttctcag actatccacc tttgggtcgc tttgctgttc gtgatatgag 420 gcagacagtt gcggtgggtg tcatcaaagc agtggacaag aaggctgctg gagctggcaa 480 ggtcaccaag tctgcccaga aagctcagaa ggctaaatga atattatccc taatacctgc 540 caccccactc ttaatcagtg gtggaagaac ggtctcagaa ctgtttgttt caattggcca 600 tttaagttta gtagtaaaag actggttaat gataacaatg catcgtaa 648 // ID JK123908; SV 1; linear; mRNA; EST; MAM; 698 BP. XX AC JK123908; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI14FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-698 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; c923f56ef569aa88496fcd58b87fdcf1. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig1239 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000002278 (LOC100415693) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000012111 CC High quality match with Homo sapiens transcript: ENST00000316149 CC (NDUFAF4-001) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000026488 (XM_001100962.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000017268 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000034071 (XM_001135738.1) CC High quality match with Pongo abelii transcript: ENSPPYT00000019615 CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0BOF8H01CHU3P, F1GITI001AM2DX, F1BDRJG03G39DW, FZ8A1BE01A0BM1, CC F0DRJ6O01EADTM CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874246|FR874246.1:123728224-123727559. XX FH Key Location/Qualifiers FH FT source 1..698 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 698 BP; 234 A; 140 C; 127 G; 197 T; 0 other; ggacataacg ctatgaggag atgggggcct tagtgattcg cggtatcaga aatttcaacg 60 tagagaaccg agcggaaagg gaaatcagca agataaagcc ctctctcgct cccaggcacc 120 cctctaccag caacctcctg caagagcaga ctagtcacta tccagaaatt aagggagaga 180 ttgctcgtaa agatgacaag ctgctgtcat ttctaaaaga tgtgtatgtt gattccaaag 240 atcctgtgtc ttccttgcag gtaaaagctg ctgaaacatg tcaagagcca aaggaattca 300 gattgccgaa aggccatcag tttgatatga taaatatgaa gagcattccc aaaggcaaaa 360 tttccattgt agaagcattg acacttctca ataatcataa actttatcca gaaacctgga 420 ctgctgagaa aatagtgcaa gaataccagt tagaaaagaa agatgtgaat tctcttctta 480 agtattttgt tacttttgaa gtcaaaatct tccctcctga agacaagaaa gcaatacaat 540 caaaatgaag aaaatcacaa aaatttccta tgtgtactcc tcatccctca tcctgtatat 600 tttctcgttt tttgcatatt aaattatgtt aattaccaaa tatttaatgc tctcatcatg 660 aggccatatt cttaatattt attgagctcc ttgacttt 698 // ID JK123909; SV 1; linear; mRNA; EST; MAM; 550 BP. XX AC JK123909; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI15FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-550 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 018766bbe35360237edab7e46b91e96e. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig3120 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000061869 (B0KWN2_CALJA) CC High quality match with Gorilla gorilla transcript: CC ENSGGOT00000015433 CC High quality match with Homo sapiens transcript: ENST00000398881 CC (TOMM6-002) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000017410 (XM_001084780.1) CC High quality match with Nomascus leucogenys transcript: CC ENSNLET00000006205 CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000065552 (XM_001174258.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0Q2XTE01BIH9E, F1HESOY01DA8KF, F1RTSFR02G4M03, F0BOF8H02FORM7, CC F0Z1IVZ01EI8XX CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/) CC High quality match with the Macaca fascicularis draft genome at CC location: ENA|FR874259|FR874259.1:60361664-60361113. XX FH Key Location/Qualifiers FH FT source 1..550 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 550 BP; 138 A; 134 C; 133 G; 145 T; 0 other; agcggggtcc cggtgagcgc tgctggctcg gctaatgaaa ctcccgaaat accagacaac 60 gtgggagatt ggcttcgggg cgtctaccgc tttgccacag ataggaatga cttccggagg 120 aacttgatat taaatttggg actctttgct gcgggagttt ggctggccag gaacttgagt 180 gacattgacc tcatggcacc tcagccaggg gtgtagccaa gtagacaaat ggaatcctgt 240 gctgaacccg aatcttccaa aaaacagcct acaatctgtg accaccacaa gatgtgccct 300 gatggcagct gaagtttgat tcagatgggc actttcttcc ctctccctgc ctagtttcct 360 tttgttcctt gagtccatgc agaattccat tctctggtca gcagacaggc ttaagctaaa 420 gtatggcctc tgttctataa agttctgtac atagttccca agcctctgca gggggtgatc 480 tttgctcttg tcctgagaaa taacagtgct gttttaacaa acatttgaaa taaataccac 540 acacaaaggc 550 // ID JK123910; SV 1; linear; mRNA; EST; MAM; 643 BP. XX AC JK123910; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI16FM1 Chlorocebus sabaeus PBMCs EST Library Chlorocebus sabaeus DE cDNA, mRNA sequence. XX KW EST. XX OS Chlorocebus sabaeus OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; OC Cercopithecidae; Cercopithecinae; Chlorocebus. XX RN [1] RP 1-643 RX PUBMED; 22726727. RA Tchitchek N., Jacquelin B., Winker P., Dossat C., Da Silva C., RA Weissenbach J., Blancher A., Muller-Trutwin M., Arndt Benecke A.; RT "Expression sequence tag library derived from peripheral blood mononuclear RT cells of the chlorocebus sabaeus"; RL BMC Genomics 13(1):279-279(2012). XX DR MD5; 7e9b479a231a7a7c69b240dd7fc1b746. XX CC Contact: Nicolas Tchitchek CC Systems Epigenomics CC Institut des Hautes Etudes Scientifiques CC 35 route de Chartres, 91440 Bures sur Yvette, France CC Tel: +33160926683 CC Fax: +33160926609 CC Email: nicolas.tchitchek@ihes.fr CC Assembled in contig: Contig189 CC High quality match with Callithrix jacchus transcript: CC ENSCJAT00000010664 (LOC100394350) CC High quality match with Homo sapiens transcript: ENST00000320936 CC (CIRBP-201) CC High quality match with Macaca mulatta transcript: CC ENSMMUT00000031090 (LOC706175) CC High quality match with Pan troglodytes transcript: CC ENSPTRT00000018713 (XR_020876.1) CC High quality match with Chlorocebus sabaeus draft genome reads: CC F0X5I7102FX4TV, F0BWDJC02JWBEI, F0X5I7101ACS1F, F1GITI001BRZ2Q, CC F0BWDJC02JWBEI CC (http://www.genomequebec.mcgill.ca/compgen/vervet_research/). XX FH Key Location/Qualifiers FH FT source 1..643 FT /organism="Chlorocebus sabaeus" FT /mol_type="mRNA" FT /sex="male and female" FT /dev_stage="adult" FT /clone_lib="LIBEST_027323 Chlorocebus sabaeus PBMCs EST FT Library" FT /cell_type="Primary Peripheral Blood Mononuclear Cells FT (PBMCs)" FT /tissue_type="blood" FT /note="16 different AGMs SIV infected or not were used for FT this library. PBMCs were isolated from blood by FT Ficoll-Hypaque (Pharmacia Biotech) density gradient FT centrifugation. For Concanavalin A (ConA) activation: 4 FT 10^6 of isolated PBMCs were plated in RPMI-1640 with 5% FT fetal calf serum (FCS) with 10 g/ml of ConA for 2, 6, 24, FT 36 or 72h. For lipopolysaccharides (LPS) activation: 4 10^6 FT of isolated PBMCs were plated in RPMI-1640 with 5% fetal FT calf serum (FCS) with 10 g/ml of LPS [E.Coli 0111:B4 Sigma FT (L2630)] for 2, 6, 24, 36 or 72h. The mixed lymphocyte FT reactions (MLR) were done by mixing 4 10^6 of isolated FT PBMCs with 4 10^5 PBMC from another animal in RPMI-1640 FT with 5% fetal calf serum (FCS) for 2, 6, 24, 36 or 72h. FT Unstimulated cells were also kept for further RNA FT extraction. Total RNA was extracted from 4 x 10^6 cells by FT using the RNeasy Mini extraction Kit (Qiagen, Courtaboeuf, FT France) following the manufacturer's instructions." FT /db_xref="taxon:60711" XX SQ Sequence 643 BP; 136 A; 149 C; 222 G; 136 T; 0 other; cgggtcgttg tgctgtgccg tcttcccgct tgcgtcaggg acctgcccga ctcagtggcc 60 gccatggcat cagacgaagg caagcttttt gttggagggc tgagttttga caccaatgag 120 cagtcgctgg agcaggtttt ctcaaaatac ggacagatct ctgaagtggt ggttgtgaaa 180 gacagggaga cccagagatc tcggggattt gggtttgtca cctttgagaa cattgacgac 240 gccaaggatg ccatgatggc catgaacggg aagtctgtag atgggcgaca gatccgagta 300 gaccaggcag gcaagtcgtc agacagccga tcccgtgggt accgtggtgg ctctgccggg 360 ggccggggct tcttccgtgg gggccgagga cggggccgtg ggttctctag aggaggaggg 420 gaccgaggct atgggggcaa ccggttcgag tccaggagtg ggggctacgg tggctccaga 480 gactactata gcagccggag tcagagtggt ggctacagtg accggagctc gggcgggtcc 540 tatagagaca gttacgacag ttacgctaca cacaacgagt aaaaaccctt cctgctcaag 600 atcgtccttc caatggctgt gtgtttaaag attgtgggag ctt 643 // ID JK123911; SV 1; linear; mRNA; EST; MAM; 577 BP. XX AC JK123911; XX DT 13-JUL-2012 (Rel. 113, Created) DT 04-FEB-2014 (Rel. 119, Last updated, Version 3) XX DE PP0ADA92YI17FM1 Chlorocebus sabaeus PBMCs EST Library