ID DY238988; SV 1; linear; mRNA; EST; PLN; 447 BP. XX AC DY238988; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D05.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-447 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 30fe01b58d7bc6fd99ccfd36cb263db8. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 05. XX FH Key Location/Qualifiers FH FT source 1..447 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 447 BP; 77 A; 152 C; 153 G; 65 T; 0 other; gaacaaacca cacaccccac ctaccccggc cggaccggca ggcagaacag gatggacctc 60 gccctcctaa agaaggctcg ctgctgggcc tgttcgccgc ggctgtggtg gccatcgccg 120 tggccaatct gaccggcaag cggtaccgcc tcccaccggg gcccccgggc gcccccgtgg 180 tgggaaactg gctgcgggtg ggcgacgacc tgaaccaccg caccctgatg gccatggcga 240 agcggttcgg cgacatcttc ctgctgcgca tgggcgtgcg caacctggtg gtggtgtcga 300 ccccggagct ggccaaggag gtgctccaca cgcagggcgt ggagttcggc tcccgcaccc 360 gcaacgtggt gttcgacatc ttcacgggca aggggcagga catggtgttc acggtgtacg 420 gcgaccactg gcgcaagatg cggcgca 447 // ID DY238989; SV 1; linear; mRNA; EST; PLN; 415 BP. XX AC DY238989; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D06.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-415 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 9abdc6d3798d0c6f90723c845155fd26. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 06. XX FH Key Location/Qualifiers FH FT source 1..415 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 415 BP; 91 A; 120 C; 123 G; 81 T; 0 other; gtcaccatat ccgcggtaag gtagtttcta aaaagatttt ttcatcccct cgccgcccgc 60 cgcccgccgc gattcacccc cgccaggtcg ccacgcccgc agcccgcaga cgcaaagccg 120 cgcgcgaggc ctctcccctg cgtacgcgga gatgaagatc gtccaccgcg acctcgttcg 180 caacgggcct ggctccgtca agttggtgcc ggaggaggag gaagacttgt ggcacgcgta 240 caacctcatt gccattggag acagcctcca ggccgtcacc gtacggaaag tgctgagaga 300 ggtggcatct ggaggacgtg atgccgagcg tgtgaagctt aagttagaaa ttgtagtcga 360 gtctgtagac tatgacaagg agggatctgt tttacgtgtg cgtggaaaaa atatc 415 // ID DY238990; SV 1; linear; mRNA; EST; PLN; 540 BP. XX AC DY238990; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D07.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-540 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 500caba519d045dd363e32880ff0aa37. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 07. XX FH Key Location/Qualifiers FH FT source 1..540 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 540 BP; 55 A; 212 C; 176 G; 97 T; 0 other; cgccgtggcg ttcctcgtcg ggttctccag gggggcgtcg gggacgctcg acaacgcgct 60 ctacccggtg ctgttcctcg actacctccg ctccggcggc ggggtggcgc tgccgccgcc 120 ggcccgctcg ctgggggtcc tcgcgctcac ggccgcgctc acctacctca tctacggggg 180 gctccacatc gtcggcctct cggcgctggc gctcaccgcg ttctcgctct ccccgttcct 240 cgcgctcacc gtgctcgcgg cccccaagat ccgcccgtcc cgctggctcg ccatcgacgc 300 ccgcgccgtc gacctcgggg gctacttcaa ctccgtgttc tggaacctca acttctggga 360 caaggccagc acgctcgccg gcgaggtcga ggaccccagg aagacgttcc ccaaggcggt 420 gttcggcgcc gtcgggctcg tcgtcggcgc gtacctcatt ccgctgctgg ctggtaccgg 480 tgcgctgccg ccagagacgg cggccgagtg gaccgacggc ttcttctccg aggtcgggca 540 // ID DY238991; SV 1; linear; mRNA; EST; PLN; 305 BP. XX AC DY238991; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D08.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-305 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; e45a2135afbca6e6c726d74a3cef850f. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 08. XX FH Key Location/Qualifiers FH FT source 1..305 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 305 BP; 91 A; 74 C; 49 G; 91 T; 0 other; aatcatatac tggtacacgt tctccagttc tggattatat ctggaggtga ccatccatcc 60 taacaataaa atgtgtacca aaatccagct ctctatctac tctacgtatg catccataat 120 aagtacttca aaccgaaaag gacaacatca gatacgccta catgtttgag agatctacgg 180 gcagacaact ctatatgtac tgacagtgtt ctttacatca aaactgaatg ttctcttgtc 240 tgtcttttgg ggcaacacac tgccatagag ctctgtaaga atcaatctct ctcgcgtcta 300 ttcct 305 // ID DY238992; SV 1; linear; mRNA; EST; PLN; 373 BP. XX AC DY238992; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D08.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-373 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 9f1745f9248b89a6d18f42e6fd224683. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 08. XX FH Key Location/Qualifiers FH FT source 1..373 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 373 BP; 115 A; 66 C; 84 G; 108 T; 0 other; gcaacttatt tcaaggaata caacatggtt ttcatctggt ttaacggcac gtaaagcttc 60 tgacgggagc ccgaattctc ctgcagatgc tgagaggggc actgtctata cctatagaag 120 atttcatata tataatgcgt gatgtgcatg ctgtatttgg agagctttca aaatcaggca 180 gcttctttgg acatgtgaat catttactag ggtcatgccc cattgaagta tttaatcttg 240 tgaaacaaag tatactgcaa gctgttgaac ctttaaagga acggttgcct gcaataatca 300 gtgttatgat tggaatcata gttaagaagt ctaacgagga cttgaagcac ctgaaaggaa 360 taacagcgac ata 373 // ID DY238993; SV 1; linear; mRNA; EST; PLN; 546 BP. XX AC DY238993; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D09.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-546 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 3884cdd1c388bd2cbd99944e658f3322. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 09. XX FH Key Location/Qualifiers FH FT source 1..546 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 546 BP; 104 A; 172 C; 169 G; 101 T; 0 other; gatccaaaga ggtatcgaga taagtgatga taacttcacc ccatcatcaa acacatacgt 60 gcagatccta agcaagccct gcatgctcgc cgcgcacata gttgcattgc ggacatacac 120 acggcgactg ccgcctagct agttctgcct gatgacgtgc ccctcatttg ctttcttgaa 180 cagacgcgcc tgtgccgcgg cggcgccgtc gtcaccagag ttgagcgcag caccgaatgc 240 gtccttcacc tcctgcgtcg tgaaggggaa cctgttgttc accatggagt tgagcaacga 300 cgccgtgccc tcgcggtaga gcgcgcccat gccgtcctcg cgcgtgttcg ccagcgcctc 360 cggtacggtg aggtcgtgcc cgaacggcac cgccgcgctc gggccgaaca gccgcaccag 420 cgggcaccag aaaccgaata ggccccagat cacgcccggg tgcatcatcc agtaggtgca 480 tgggcccgtg aagggcggcg tgttggggtc gtacggcgtt gggggagtcg gtgttggcgt 540 cagtgg 546 // ID DY238994; SV 1; linear; mRNA; EST; PLN; 453 BP. XX AC DY238994; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D09.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-453 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 067d833e6d7e308dcb2ef56199393d39. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 09. XX FH Key Location/Qualifiers FH FT source 1..453 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 453 BP; 80 A; 169 C; 127 G; 77 T; 0 other; gcagctagta aggctagctg gaaagctcac taccctgtaa acctccagca agcttcagct 60 agctcattgc ctccttgcaa ggagtgtgga gatggctact aggccggctc tcgtgttcgt 120 ttgctgctgc ctcttgctgg cgtacgccgc ggcagcgcgt gtgctcgaag agggagagcc 180 ttttggccgc ctgaaaaatg ctgcgcgccg tctccgcgat ggagagcctt tcggcggccg 240 gaaggacccc gccgacccgg accactctcc cggctcacac gggactccgc cctgcccgcc 300 gtccatcggc acctcgcaga ccacgccaac cacacccggc ggcggcagct actacccgcc 360 ttcgccagac atcggcactt ccccgaccac accgacagct ccaggcggta gcggcgggta 420 agatgctccc ccgtcaccgt ccagcgacac ctc 453 // ID DY238995; SV 1; linear; mRNA; EST; PLN; 469 BP. XX AC DY238995; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D10.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-469 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; f67d24096a35dcbe750adb5827931556. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 10. XX FH Key Location/Qualifiers FH FT source 1..469 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 469 BP; 97 A; 116 C; 176 G; 80 T; 0 other; tgaagaaggg gcacagcagc ggcaggattt gggtgccgcc gcccgccgtg acggacaccc 60 tgatgagcta cttcgaggag aacaaggacg tctgatgggg ccgagcggta cgtgggggct 120 ctgaaaaggg tgcaaaagga tctgggcgca ccggtgttcg agccgctggt gcggacgtac 180 gctgccgccg ggaaaaagtc ccccgggatg cggcagcggc tgaagatcga gaacgtggag 240 gtcggggagg ggacggccca gctgcttgac tctatctgcg ccgatcaata ccccaagcac 300 gtcctagagg cggtggcagc gttgggggcg gctgctatgc gatgggggca ccacctccgt 360 tgatgtattg tctggtgcgg gactgcgagt gacctctgag gcgggggcgg agaataactg 420 gatctaccta acgtcggagt ggagacctgc aggtccattt gaaattaaa 469 // ID DY238996; SV 1; linear; mRNA; EST; PLN; 313 BP. XX AC DY238996; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D11.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-313 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 047d250b1798339cf1f3efd940876f5e. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 11. XX FH Key Location/Qualifiers FH FT source 1..313 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 313 BP; 119 A; 65 C; 47 G; 82 T; 0 other; aataaaaaaa caattttttt actccaatcc aaaaaaggct tcctaaataa aatctcttta 60 catgaaaata aaggtcttca acaaacatta ttcaggaatt gatacaacct ggatatgcat 120 aaaaacagat aaagtaggaa ctacaaacta gtcagcctat acccccccca ggtaacttgt 180 tttattgcca agcgttgatc gtaccatacc aactgaagct gaaggcctct cattcagctt 240 attcgggttt tcactgaaaa ttctatatat ccccagcaga aagggaaggg aaaggaaagt 300 gtataactaa tcc 313 // ID DY238997; SV 1; linear; mRNA; EST; PLN; 550 BP. XX AC DY238997; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D11.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-550 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; b7f367803ba329e4a80aa2e46159cc50. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 11. XX FH Key Location/Qualifiers FH FT source 1..550 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 550 BP; 152 A; 123 C; 141 G; 134 T; 0 other; cgatcgccgg gcaatgggag ctgggcgggg gcggtgatgg ccgcggcggc ctccaggttg 60 gagaaggcgc tgggcgagca gttccccgag ggcgagcgct acttcggcct cgagaacttc 120 ggaaacacct gctactgcaa cagcgtgctg cgggcacttt atttttgtgt ccctttccgt 180 gatcaattgc gggagtacta tgcaaacaat aaaaacaccg gagatgttga agagaacatg 240 ctaccctgcc tgggtgatct gttctctcag atcagtaccc aaaaaaaaaa aacaggggtt 300 attgctccta agcgttttat acaacgattg aagaaacaaa atgagatttt ccgcagctat 360 atgcatcggg atgctcatga gtttctgaat tttttactga atgagctagt tgacattcta 420 gagaaagaac atagtgctgc aagagagtct cttcaaaatc tctcactcca aaagaattca 480 aatggcccta ttgatggcca acccaatggt agtcacaaag aattatctgt cacatgggtt 540 cataagtgct 550 // ID DY238998; SV 1; linear; mRNA; EST; PLN; 419 BP. XX AC DY238998; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D12.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-419 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; ed48f31da741b9f3b3714eac9595e4b3. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 12. XX FH Key Location/Qualifiers FH FT source 1..419 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 419 BP; 78 A; 144 C; 115 G; 82 T; 0 other; aaaccccacg cgagcacccc attcccatcc atggcgacca ccacctcttc ctcctcgatg 60 accactcctc tcctccgccc gaaccccaat accaaccctg ctcccagatc tctccaactc 120 ctcaggagca ggaggtgcgc tcgtgccgta actaccgccg tacccggagg tgcggtgcct 180 tacggggcta atccgcggcg cgggatttgg tcgatcaggg atgacttggg ggtgccaagg 240 tcgccctact tcccagtgga gtccgcggcg gggcaggagc gtgggccgtc gcccatggtg 300 atggagcggt tccagagcgt cgtcagtcat cttttccagc acagaattat tcggtgcgga 360 ggccccgttg aggatgatat ggccaacatc attgtagcac aactgctcta cctcgacgc 419 // ID DY238999; SV 1; linear; mRNA; EST; PLN; 419 BP. XX AC DY238999; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D13.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-419 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; b3f6962b63393aafd32bf7f579da32ef. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 13. XX FH Key Location/Qualifiers FH FT source 1..419 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 419 BP; 82 A; 134 C; 138 G; 65 T; 0 other; gggggcagcc ggctgcaggg atttcttttc cgattttgct ggccaaaatc gactggacac 60 tcaagctggg ttgctaggtg gtgatcagag cagaagcaca gctttgatag gagggaaggc 120 taaaacactg gtgggggtga acttgtcgct gacgtcgacc tggtccgccg acatgccggc 180 cggcagctgc cactggaacg cgtgcaccag cgacgctaga accagcggca ccacccgctc 240 cgccatgggc acgcctggcg gcctgggcac agcctacggc cggagccgaa cgggacgaac 300 tccagctgct tcccgcggaa gtccaggtcc ctggccagga accgctccgg caggaactcg 360 ccgggcctct cccacgccgc cgggtcgcgc atgatcgccc acgaattgaa gatcacggg 419 // ID DY239000; SV 1; linear; mRNA; EST; PLN; 492 BP. XX AC DY239000; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D13.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-492 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 2b50540e1b5ff63638e6d3fb5add0b22. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 13. XX FH Key Location/Qualifiers FH FT source 1..492 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 492 BP; 52 A; 218 C; 151 G; 71 T; 0 other; tggatgacga cgatgctggc cgtcgtcttc tcgaccgtct tctgctacct gaccaaccac 60 caccgccgct gggggggctg ggcctcctcc tcagggcgtc gtcggccgcc gggcccgcgt 120 cctctcccgc tcatcggaaa cctggtggac ctgcggcgcg cgcccggcac cctccaccac 180 acgctggcgc gcctggcccg cgcgcagggc ccccccgtca tgcccctcga cctgggcctg 240 gtcccggccg tggtcgtctc ctcccgccac cccgcacggg aggcgttcgc ggcgcacgac 300 cgccacatcg cggcgcgccc cgtcccggac agcaagcgcg cgctcggctt ctgcgaccgc 360 tccatgctct cactgcccag ctcggccccg ctctggcaga ccctgcgcgg cgtcatggcc 420 gcacacgtcc tgtcgccgcg cagcctcgcg gcgtcgcgcg ccgcgcggga acacaaggtc 480 ccccacctga tc 492 // ID DY239001; SV 1; linear; mRNA; EST; PLN; 359 BP. XX AC DY239001; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D14.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-359 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 3fa5d324614662ca7d1fb7663fba266b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 14. XX FH Key Location/Qualifiers FH FT source 1..359 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 359 BP; 77 A; 104 C; 110 G; 68 T; 0 other; ggcaacacga atgatcagtc ttttaccaag atcgcttcgg gacgaagcgg cctacacgaa 60 ctaccgcaca aaatagactt ggaaagccgt ttttacacta cttcttcgcc gcgccgtcta 120 aaagcgaaca aacagcataa agttacaaat tgcactcctc catccctccg ccgccactgg 180 ggccgggcgc gagatacgcc tacgcgcgct cgccgcggat gcggcgggcg agctggatgt 240 ctttgggcat gatggtgacg cgctttgcgt ggatggcgca gaggttggtg tcctcgaaca 300 ggccgacgag gtaggcctcg gcggcttcct ggagggccag gacggcgtgg ctctggaac 359 // ID DY239002; SV 1; linear; mRNA; EST; PLN; 425 BP. XX AC DY239002; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D14.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-425 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; d496ddf8628eee1874c8f3f59e2a8e3a. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 14. XX FH Key Location/Qualifiers FH FT source 1..425 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 425 BP; 104 A; 143 C; 126 G; 52 T; 0 other; ctcaaattcg ttccgtccag gcaaggaaaa aatcgagcag ccaaggagag gagaagatgg 60 ctcgtacgaa gacaaccgcc cgcaagtcca ccggaggcaa ggccccgcgc aagcagctcg 120 ccaccaaggc ggagagaaag tcggcgccga cgaccggcgg cgtgaaaaac ccccacaggt 180 acgggcccgg gacggtggcg ctgcgcgaga tccgcaagta ccaaaagagc acggagctgc 240 tgatccggaa gttgcccttc cagcgcctgg tccgtgagat cgctcaggac ttcaagacgg 300 atctgcggtt ccagagccac gccgtcctgg ccctccagga agccgccgag gcctacctcg 360 tcggcctgtt cgaggacacc aacctctgcg ccatccacgc aaagcgcgtc accatcatgc 420 ccaaa 425 // ID DY239003; SV 1; linear; mRNA; EST; PLN; 388 BP. XX AC DY239003; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D15.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-388 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; c49c23d814c50ea026b14651946c6da1. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 15. XX FH Key Location/Qualifiers FH FT source 1..388 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 388 BP; 75 A; 124 C; 121 G; 68 T; 0 other; caaaaagatc ccatccacta ccgcccggtg gcggtcctcc gccctccgcc ctccccgccg 60 ctcgttcggt gcaggtcgcc ctcgcgtgga tcggaatggg cgcctgcgtg tccaaggccg 120 cgtgctgctg ccgccagccg cacaaggggg ttaccaacga gaggactgat actgtgacgg 180 aggaagagga ggcatacgac ctgcctgcgt tccaagagtt caccttcgag cagttgcggc 240 tagctacatc ggggtttgcg gtggaaaaca tcgtgtcaga gcaaggagag aaggcgccca 300 atgtggtata caaggggaag ctcgatgccc agcgccgcat tgctgtcaag cgctttaacc 360 gctctgcctg gcccgacccg cgccagtt 388 // ID DY239004; SV 1; linear; mRNA; EST; PLN; 378 BP. XX AC DY239004; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D16.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-378 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 98a797d4af378dad966355ea7aee7df4. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 16. XX FH Key Location/Qualifiers FH FT source 1..378 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 378 BP; 96 A; 93 C; 106 G; 83 T; 0 other; ggagtccatt cattcattca ttcattcatt catgttgttg cttatacttc cgaaaacatt 60 aatcaacgca aataagaaaa acaagaggaa taaatttaca ggcatagtat acgtttagga 120 acgacgactt ttgtgtagtc tgatcgattt gaagccaaaa caaaagcatg attacgtgac 180 gaggatgagg ccctcggtga tgagcgagtc agcgatgacc tggttggccg cctcggacgg 240 gtggacggcg tcccagaaga cgtaggacgt ggcgttgggg cacgtcccga cggacttggg 300 gttgcagaag agcacggtgg tctccaccgt gcccgtcccg cagcatcccc gccgggcctc 360 cgcgaagccc tgcgactg 378 // ID DY239005; SV 1; linear; mRNA; EST; PLN; 429 BP. XX AC DY239005; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D16.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-429 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; b2e87283229fa45b0180793c3168126b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 16. XX FH Key Location/Qualifiers FH FT source 1..429 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 429 BP; 69 A; 142 C; 134 G; 84 T; 0 other; gtgcgttgcg tcgggggtgt ttgtgtttca aagctaatag tctgatactg aaaaagatag 60 aggtgatgat ggctctgcag gcgcagggga cgcgggtgcc gctggtcctg gcgctcttcc 120 tcctcctggc cggttgcggc agcgaggcgc acccgctggt gccggcgctg tttactttcg 180 gtgactcgtc gggggacgtc gggaacaacg actacctcca ccccatcatc agggccaact 240 tcccgccata cggcggggac ttccccaacc acgtcccccc cgggaggttc tgcaatggca 300 agctggcgac cgacatcccc gccgacacgc tggggttcac tacctaccct gccgcgtacc 360 tcagcccaca ggcgtcgggg cagaacctcc tcatcggcgc caacttcgcg tccgcggggt 420 ctgggtact 429 // ID DY239006; SV 1; linear; mRNA; EST; PLN; 574 BP. XX AC DY239006; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D17.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-574 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 5354cdab66188326322a27da0c69e2da. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 17. XX FH Key Location/Qualifiers FH FT source 1..574 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 574 BP; 124 A; 147 C; 143 G; 160 T; 0 other; cgagcgagag agcggaagac gagtcgcggc gcctctgcgg tgctgtcttc ttctccccct 60 ctgctctgct tcggctcctc acgcggcctc gccgagaccc gcaaaacagg gggttcagac 120 ttcttggtga gagcgatatg agtgtcccag gtcagtgctc atcccaccca cattttatca 180 ccagcgttgg gaggagtaac cgctccagtg gccccggcac tcctcttatc gaaagcatag 240 atgttgatca atttgttatt ccagagaaaa acagttggaa gaacttattc tcatacatag 300 ggcctggatt tctcgtctca attgcatata ttgatcctgg aaactttgag acggatctac 360 gggctggagc acagtacaaa tatgaggtgc ctgctgcttc agctcttatg ttcccacttg 420 tgcgtcttct ttggatcata cttgttgcat cctgtgctgc tcttattatt caatcacttg 480 cagccaggct aggggttgtg acagggaaac atcttgcaga gcattgcagg gctgagtatc 540 ccaaggtcac aaattttgtc ttatggattc ttgc 574 // ID DY239007; SV 1; linear; mRNA; EST; PLN; 490 BP. XX AC DY239007; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D19.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-490 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; d2bf12d85d5da83e33f9e04b577006bc. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 19. XX FH Key Location/Qualifiers FH FT source 1..490 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 490 BP; 148 A; 134 C; 111 G; 97 T; 0 other; agctagttcg gaatagcatt aaagatttaa agttcaccat ttatacatct cagtgatcac 60 taggaaaaaa aataattttc acgactcaca acaatactat atatgctgca tgcctgtatc 120 caaaggcacg ataaaaaaaa tacatgcttg atttaggaaa agctaggtgt acaacaaccg 180 cccacacgtt gctagctgca gggctgcttt ttaatcacaa atccacaaag aagagcaaga 240 atcatatacc cgtagaaaaa aaaagaatac ccaacaaaac aaagaattaa ccggcggacg 300 catatcagtg gcgggcgcac tcggcgggga gtccttgcgg gaacctccac ccatcctggc 360 agtagttgta gcgcatgaag ttgcgctccg cccacctgac ggtgccccac tcggcgctgt 420 caagctgcct gttcatccac ccgccgctgc cggcggcccg gcacgcgccg ccgctgacgc 480 aggggctggc 490 // ID DY239008; SV 1; linear; mRNA; EST; PLN; 470 BP. XX AC DY239008; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D19.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-470 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 9288149b25859ee7e828e7f0a79db7f2. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 19. XX FH Key Location/Qualifiers FH FT source 1..470 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 470 BP; 93 A; 154 C; 143 G; 80 T; 0 other; ccaagaagtt ccctccagtt tgtaacctcg tttccaaaaa gagctctaga gccgccacac 60 acacacaaac accatggcac cgcgttctct gctggcagtg ctcgccgtcg ccgccctcgc 120 gttcttgcac gccgcctcgg ccaagtcgtc gtggcttgac gggaggttca ccacggacgg 180 caccgtccgc acgggatacg acgattcggg ccagcagggg gagatgctcc gcctcgacca 240 gcactccggc gctgccggtt tcaactccaa ggagcagtac ctttacggcg agttcagcgt 300 cgagatgaag ctcatccggg ggaactccgc cggcaccgtc tcgtgcttct acctctcttc 360 cggagacgga gacgggcgcg acgagatcga catggagttc atgggcaact cgagcgggca 420 gccggtggtg ctgaacacca acgtgtgggc cagcggcgac ggcaagaagg 470 // ID DY239009; SV 1; linear; mRNA; EST; PLN; 625 BP. XX AC DY239009; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D21.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-625 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 7d82c452b3522d6af9bd3d20c4185d95. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 21. XX FH Key Location/Qualifiers FH FT source 1..625 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 625 BP; 185 A; 128 C; 124 G; 188 T; 0 other; acatcaaaac acattattat ataaatataa ttaagttggc attatccata ttgtcccaac 60 ccaggggaat gccaaagcag gggttggggc caaggggcat gcctgatata caatcaaaac 120 tatgatcagc tgagaatcaa ccagttgcag aaccatcttt tttcatagtt ttagaaaacg 180 aaaatatgat atacatttga gtatattttc tagcaatcac caatatgatc ttgcgattga 240 aatcaatagc ttgtgctggt ggttaaaaat aggatcgcga tgagcgtgat gaacgggtgg 300 tgctgccctc attgcgatct ttcttccaca ttccttcaat gctcaggaaa cacttgagag 360 gtctcgcctt ccaacggttt ctgtgagcta tctcattttg gatagcagac agccggttga 420 taattactct aaaatcaggc ctgtctgcag aattttcgct ccagcactgt tcaatcaact 480 ctcttaaccc atgggcataa tgctttggag gagctcgaaa aggaggcctt tctttagaat 540 tatgtgcctt ctcaatttca tcatttttct tatcataata aggaagggat ccttcaatca 600 tctcctgaag tatcagagcg aatga 625 // ID DY239010; SV 1; linear; mRNA; EST; PLN; 511 BP. XX AC DY239010; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D21.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-511 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; bf344f3581e99b2b56feb9c9d6bea41b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 21. XX FH Key Location/Qualifiers FH FT source 1..511 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 511 BP; 160 A; 93 C; 118 G; 140 T; 0 other; cgcgaagggt gacttgcgca ctctcttgag taaaaaagga gctctgccac catcttatgc 60 tgtgaagtta gctcttgata ttgcaagagg agatgaatta tttgcgtgag cataaccctc 120 gggctatcat ccatcgtgat cttgaacctt ctaacatatt gagggatgac actggacatc 180 tgaggggggc agattttgat ctatgtaaga tgctaaaatg gaaaaaaaaa gttagaaaag 240 ataaagcact ctcttctcca ggcaatgctt gtaaatatgt ggctccaaaa gtcctgcgaa 300 atgaagagta tgataccaaa gtggatgtct tctcattctc tctgatactt caggagatga 360 ttgaaggatg ccttccttat tatgataaga aaaatgatga aattgagaag gcacataatt 420 ctaaagaaag gcctcctttt cgagctcctc caaagcatta tgcccatggg ttaagagagt 480 tgattgaaca gtgctggagc gaaaattctg c 511 // ID DY239011; SV 1; linear; mRNA; EST; PLN; 490 BP. XX AC DY239011; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D22.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-490 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; d455008d41a4158d91d266a0b7542228. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 22. XX FH Key Location/Qualifiers FH FT source 1..490 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 490 BP; 130 A; 132 C; 92 G; 136 T; 0 other; gtgaatttta cttcatttcc cacggaacta aactactctt tcctcttgtg atgcaagtat 60 tctgatagcg tgttttacat gaggacccac gtaaaatggt acaagtacag aatcctcaaa 120 aaaaaaaaga ggaaccaaaa ggaaaacaaa ttctgcaaac aaacggttca ccaattgcct 180 actggcacca gataatcctt caaagccttc accttcttct tgctccttag tttccggaac 240 gcgccagact cgatctgccg gattctctcc ctgctcacac ccatgatgtt accgatctcc 300 tggggggttc ttatccgacc atcctcgagc ccaaacctca gaacaacaac ttgcttctcg 360 cgagtggtga gagtatctag tgccatgtgt acctccttct tcatggaaat tctgttgagc 420 atctcttcag ctgtctcagc actgggatca gctgtgacct cctgatatgt catgtcggtg 480 gaaccaattt 490 // ID DY239012; SV 1; linear; mRNA; EST; PLN; 473 BP. XX AC DY239012; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D22.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-473 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; b04db9e01bf97b76508129002597d6cd. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 22. XX FH Key Location/Qualifiers FH FT source 1..473 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 473 BP; 101 A; 151 C; 134 G; 87 T; 0 other; cggcagcaat ggaggcggca cccaggtgag gcgagcaagc agcccgccct gcctccccga 60 tggggtgcct ggcgccgcag ttcaagtggg cgccgtcggc cgccgcccac atgccttcct 120 cgtcctcgtc ctcgtcctac tccaggtgct ccgcgctccg cgtccagtgc gccgtcccct 180 ccgccgctgt cgtcgaggac cgcaccaacg gcagcgcggc gcatctgagg ctcgtttacg 240 cggctccggc gattcagaga aactttgagg catctctggc atcaaaagct cttctaaatg 300 aagaggcggt agtagcagcg gcagccgcag aggctgttgc tcttgccaga gcatctgccg 360 aatctgccca agaatttgtt cagatagtac aaagaaacaa ccatcagccg gtaattagac 420 caaaaaagtg tgtggacaac tacttggcaa atgaaatcct tcgcacagag atg 473 // ID DY239013; SV 1; linear; mRNA; EST; PLN; 563 BP. XX AC DY239013; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257D23.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-563 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 8953c5114f7ecf6bd39ddb6ceb71e3bf. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: D column: 23. XX FH Key Location/Qualifiers FH FT source 1..563 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 563 BP; 160 A; 141 C; 138 G; 124 T; 0 other; aaaccctaaa cccctatttt attccatgct tggggagata caattaaaat gttggtacac 60 tttgggattc tgcgtgtgca caaaagcatt tttcctcagt caaaaatcag gtcgccggtt 120 gcgtagacgt tgtagaagat ggtggcgctg aagtagagcg ccgccgtgag cgtgaggaag 180 gcctggaagg accctagcca ctggacgaag tagccgatcc cgatggtgct aactatggct 240 gccaccgtcc ctatgccgtt cgtcagcccg tgcaaggatc cagcatactt tggagcaatg 300 tcctgtatgt tacacatgta tccggcttgg ctaaaagaac acaagctcag cgcaatggtc 360 atgaagactg cagcaactga tggagtttgg gcaaacctta agcacagcaa tgacacgcaa 420 ggccccataa aaccaatgga ctgcataatt tttccaacta gtccaataga gaaaccagat 480 ttgataagaa aatctgcaga ggctcctgca acataaccgg acatagccat gactgcccca 540 ggtatggcac tgacccacgc tgc 563 // ID DY239014; SV 1; linear; mRNA; EST; PLN; 489 BP. XX AC DY239014; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E01.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-489 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 2df425907d027f109a809015e28aaba0. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 01. XX FH Key Location/Qualifiers FH FT source 1..489 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 489 BP; 91 A; 179 C; 144 G; 75 T; 0 other; ccgcaattcc ccaatcatcg gccccgcgaa aaaccctagc ccagcgccac ctacccgtcc 60 ctctcgccgc cgccaacatg ggaggctcat ccggcggcgc agcctcctct tcctcctccc 120 gtggcgtgcc agaaaacagg ttctataatc ctccccacgt aagacgccaa cagcagcagc 180 aggggcaacg gccgttgcgg cccgcatcac cgtcgccctc gccgcggccg gtgcggcaaa 240 acccccctcc gccggcggcg gccgttgccg cgtcggcaga tgtggacatc cggggggatt 300 cagaagactc ttcgtcgaag acctcgtcca agccatcggt ggcgtccacc gcggctactg 360 caacagcggg agaggagaat gtggccgtgg cggaggcgga ggctggaaat ttggagaggt 420 tcctcgcctc cacgacgccc tctgagaccg ctcagttctt gcccatgtca agcctaagga 480 tgcggcgag 489 // ID DY239015; SV 1; linear; mRNA; EST; PLN; 529 BP. XX AC DY239015; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E02.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-529 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 34b6a41df9f0ac3b68e427dd1058565d. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 02. XX FH Key Location/Qualifiers FH FT source 1..529 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 529 BP; 92 A; 199 C; 154 G; 84 T; 0 other; tcgccttact ccctcacaga acccagaaaa atattgccag tcccgccgtc aagatggccc 60 cacgccgcct gctcgtcctc gccgtcgtcg ccctcgcggc caccgccgcc gcggccaact 120 ccggtttcgc ggactcaaac cagatccgcc ccgtcacaga ccgcgcggcc tccgcgctcg 180 agtccacggt tttcgccgcg ctcggccgca cccgcgacgc gctccgtttc gcacgcttcg 240 gtgtgaggta cggaaagagc tacgagagcg cggcggaggt ccacaagcgg ttcaggatct 300 tctccgagag tctccagctc gtccgctcca ccaatcggaa aggcctctcc caccgcctcg 360 gtatcaaccg cttcgcggac atgagctggg aggagttccg tgcgacccgg ctcggcgcag 420 cccagaagtg ctccgccacg cttaccggca accaccggat gcgcgccgcc gccgttgctc 480 tgccggagac gaaagactgg agggaggatg ggattgtgag cccagtgaa 529 // ID DY239016; SV 1; linear; mRNA; EST; PLN; 334 BP. XX AC DY239016; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E03.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-334 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 6234e07c44de099ec91f42f6c46278e5. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 03. XX FH Key Location/Qualifiers FH FT source 1..334 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 334 BP; 109 A; 71 C; 77 G; 77 T; 0 other; ctgttcattc tttggtctga atttccacca gtagcagtgt acagaagtga agagcaagca 60 tatagggtca aatctcatgg actaaattct cacacacaga atttcaaaag agagatgcaa 120 aaaagaaacc gggaggcggg attcatccca tagatacagc agaaaaatga agaccttaaa 180 aaaaatacag cgacaggaga aagaatgcta caaagggggg gggggggctt ggtttccaat 240 tgtttttatc atttccacag ctcctccatt ggaataccac gctcctcagc gacatccttg 300 aactgcttca tcatctgcgt cgccttgaaa gggg 334 // ID DY239017; SV 1; linear; mRNA; EST; PLN; 538 BP. XX AC DY239017; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E03.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-538 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 40f1fb2ca1c36c73a293dcd19eba2d33. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 03. XX FH Key Location/Qualifiers FH FT source 1..538 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 538 BP; 90 A; 205 C; 147 G; 96 T; 0 other; caccggcctc ctcctcctcc ccgtccatcg ccgtcgccgg ggcttcctac aacttcaaaa 60 tggcggctac cgccaccgcc atggctatag caactatctc ccccgccgct ccgattccat 120 ccgcgccttt cccatccttt cccctccccc tcggcctccg cctccgctct caacccctcc 180 tcttcggtgc ttcccgccgc cgcttcctcc atgttcccaa agccgcctcc tgggacaaat 240 ccgtccccga ggatggggga gatgcggagg aatccgttgc cgctggggac gacgaagacg 300 aagaggaaaa gcagcggccc gagccggtgt cctcctccga gttccagttc gcggcgccac 360 ccgagggcta cgtcgagccc gcggcgtttg acagagctac cgccggagtc cccagaagac 420 gtggcggcgg cgtaagaatc tctctacggg ccggccttca gcggagagac attgctgggg 480 aacaatgtct ttgaggtcaa ggtggtcgac cccatcgaca tggaccggga gcagcgcc 538 // ID DY239018; SV 1; linear; mRNA; EST; PLN; 348 BP. XX AC DY239018; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E04.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-348 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 9f685759eb1669d11a038f3c97930844. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 04. XX FH Key Location/Qualifiers FH FT source 1..348 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 348 BP; 87 A; 91 C; 82 G; 88 T; 0 other; cttaaaaatc cagtattgat tattggacaa caccatcaac tctctctagg ctacaaattc 60 ggcatttcac tctcgtcact cctcatggtc gcccttgtga agattgtgca ggatgtagag 120 agaagctgat gacaagaaga tgcagatcga gtagctgagg aggtccagcc ccgtggctat 180 cgtcaccatc ttgctcttct cgaacatctt cacaagtaga atcatcacga tgacgtgccc 240 cacttttgtc ttgagctcgt caagggaggt gatgttcatc cacttcgggt gttccttcaa 300 agcgaacatc ccgaacagcg acgagccgct caggggacgg tcggattc 348 // ID DY239019; SV 1; linear; mRNA; EST; PLN; 654 BP. XX AC DY239019; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E05.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-654 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 000c5613614ecf1bd89e576bc5bbf5da. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 05. XX FH Key Location/Qualifiers FH FT source 1..654 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 654 BP; 96 A; 215 C; 210 G; 133 T; 0 other; cggcgccggc gcctgctgct gcttcccgcc tttcttggac ttggatttcg accctccttg 60 gtgaaaactc cccagtacca cctgcacggt tcctgcagct accagtgggc cgcccaaaac 120 acctccgatc acattcctct cagggctgca gagcgtgacg ctgagcccgc cgctcggctg 180 gccctggccc tggcactgtt cttgttgtga gccctcggcc aggttgtacg agccggttag 240 gctcatgatc tcgtagaacc cctcgtagat ggctgggctg ttgttgtacg gctgcggcga 300 cgcgtgaacc ctcgccatgg gagagccgtc ggccgggtgg cggaggacgg ccctggagac 360 ggagcccatc gccgagagca cgcacaccgc ccttgacgac tgggtggcga acgcgacgat 420 cttcgccgcc acgtcctctg aaggagacgc cgtgatgatg tgcggagtga agctcgttcc 480 agctgaaccg gcgaacgcat tgccttgaga gggcgagggc tgatgcttct tggcggtgcc 540 cggcggccgg ccgcgccgct tcttctgcga agagtcctct atcccggcgc tgctgatcat 600 gttgggacca gacggcccta tccttggctg tgcggcgacc agctgctgct gctt 654 // ID DY239020; SV 1; linear; mRNA; EST; PLN; 636 BP. XX AC DY239020; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E05.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-636 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; dfdcb9affde1b18ced1000c508203a06. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 05. XX FH Key Location/Qualifiers FH FT source 1..636 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 636 BP; 104 A; 237 C; 186 G; 109 T; 0 other; cgccctctct ctctctctcc ttcctgcctt ctgcctgcgg gcggcccatg tccgtggcgc 60 ccctcctgcc ctgcgagctg cttctccagc gaaaaggttg ctgcgccttg acgcaccacc 120 gatccaccgc cgcccaaccc gagcgttctg tccggcagct aggtagcgag ctgcgcgggg 180 gctgcgaccg gaagcaggga gggaaacagg gcagcaaacg cccggagatt gccttgcttg 240 cctgtgtgct ccggccggcg cgagttggtc ggtcgttcca tgcatggatg acaaggaact 300 tatctcacag tccgacctgc agtcattcta ccagcagcag cagcagcacc accgggcctt 360 cggggggggc ggcggcgggc agcatacccc gtcctcgctg gccgggatgc actccgtcat 420 tcgccccatg cccaacatga acatgagccc gtcggcgatc cttaactcta tcggcggggg 480 ctccttcacc ggtatgcagt tccaaatgga cccgccgccg ccaccattgc tgcacaccgc 540 cgccatgggt gcctcggctc ctacctccac ccccggtgcc gtccccgcgg cccccacgga 600 gccggtcaag cgcaaaagag ggagaccgcg caaata 636 // ID DY239021; SV 1; linear; mRNA; EST; PLN; 387 BP. XX AC DY239021; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E06.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-387 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 5811e83b025106a1e2805b16a295afe1. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 06. XX FH Key Location/Qualifiers FH FT source 1..387 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 387 BP; 104 A; 91 C; 90 G; 102 T; 0 other; attaatatct acagtatctc attattgtca tgcaaaatgg taattacaga caggggattc 60 tgaaacctgg acacaaactt cttcctttgt tttttttctg ggagggggtt gttctgagaa 120 gggttgaaac ctgaccacac actgacgata aaggtgaaac cctggaacag aataatacag 180 gatgggcaca ggacaaacac taacgtttta ttgctactgt gtactacaca agctggagga 240 ctggagcgcc ttcaactttt catgcccatt tgctgtgcga catgcctgcc ctgcaggagg 300 agcaagcttg caatgaagct ctaatcctcc atcctcatga tgcagcgcag gctctccccc 360 ttgagcatca ggtcgaacgc cgtgttg 387 // ID DY239022; SV 1; linear; mRNA; EST; PLN; 474 BP. XX AC DY239022; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E06.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-474 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; f90ae60fcab387add72c3094e62d3f90. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 06. XX FH Key Location/Qualifiers FH FT source 1..474 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 474 BP; 111 A; 123 C; 173 G; 67 T; 0 other; agccaggtgt aaagagggcg aagaagcagt agcaaaagga ttccattctc gtgttcttgg 60 agtggtccat cgatcgagct ccgtgaaaga gagagagaga gagcaagcaa tggcgacagc 120 gggaagggag atcaagtgca gagccgccgg ggcgggggag gccggcaaac cgctgtccat 180 cgaggagggg gaggtagcgc cgccgcaggc catggaggtg cgtatcaaga tcctctacac 240 cgcgctctgc cacaccgacg tctatttggg ggaggccagg gggcaaaccc cggtgttccc 300 gaggatctta ggacacgaag cgggaggcat cgtggagagc gtcggagagg gcgtgaccga 360 gctcgcgcca ggcgaccacg tcctcccggt gttcaccgga gagtgcaagg agtgcgccct 420 ctgcaagtcg gaggagagca acatgtgcga cctcctcagg atcaacgtcg accg 474 // ID DY239023; SV 1; linear; mRNA; EST; PLN; 487 BP. XX AC DY239023; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E07.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-487 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 6b574ac8471a78aeb84fec5337a223ef. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 07. XX FH Key Location/Qualifiers FH FT source 1..487 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 487 BP; 105 A; 128 C; 126 G; 128 T; 0 other; ctgattcctc cttcttccgt tccgccgcac cgccgccgga aatatgtcga ggctcgctgc 60 tgccgccctc cgctgcgtcg tcgctgcatc gagagatacc tcctcatctc gtttcgccgt 120 gtctgcgccg gtgtctgctc ggctcttctc cgcggatgca tccggtgaac ccgccgccac 180 ggccgcagag tcacaagatg actctttctt cagggcatcc cgcgaaggat tagcctatgg 240 aaggttttac agtgtctttg gtggaggcag tcgtcttgag aagaacatat tgaaaactga 300 tattatccat caccttgaca gatgtggatt gtcattggat gatgtgaaga ttgaatacaa 360 caagggatat gctccattgg ccgctttgct gaggttttct tcgaaggcaa tgtttaccac 420 cgcaattagg caagcaaatc agaatcgtct gcacaggatt gatgagataa gtcgtgaaac 480 atgggat 487 // ID DY239024; SV 1; linear; mRNA; EST; PLN; 477 BP. XX AC DY239024; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E09.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-477 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 11992f5515dfa7fbdf7d44c6a0b2751a. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 09. XX FH Key Location/Qualifiers FH FT source 1..477 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 477 BP; 76 A; 154 C; 126 G; 121 T; 0 other; agcatctggt ctcgtctctc tccgatcctt ctcctctccc tccccctagc cactacctag 60 ttgagcgagc cctaggccct agcaggcgaa agccatggcg tccttgggtg ttcgtgccgc 120 cgccgccgct cgtcttccct ccctccgtcc tcgccgcgac ggcagcgggg tgctcaccgg 180 tttgagggtc gggacagggc actacggcgt ggggctcgcg ctttcttcgt ccacatcatc 240 ttctcccaga gagttcaggg tcgctggcct tcctgtcgat gacagcattt tgggcccggg 300 ggatgaatat gagtttggac tctatactcc taagctgctt gcagttactg gagatgatta 360 tgtgtctaac ccccgcatgc cagacgagga tgagttcgac catttctacc gtgttgtcgc 420 caagaagact gacatgctgc tcatctgcca ccttccaaag ccgcttgcag tttctga 477 // ID DY239025; SV 1; linear; mRNA; EST; PLN; 425 BP. XX AC DY239025; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E10.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-425 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 723b64354a23fa075ea1ed2f393e3dca. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 10. XX FH Key Location/Qualifiers FH FT source 1..425 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 425 BP; 95 A; 136 C; 118 G; 76 T; 0 other; cgacttcgcc gccgctccgc cgccgctccg cctcagcgca tcgccctccg ccacagcgta 60 ccgcgacctc atcgatcatc tcttcaactt tcaatcaggg gggacgtcga tgtcaaaccg 120 gaggttgccg ccggcgctcc caagaagagg acgttccgca agtacagcta ccgcggcgtc 180 gacctagatg cgcttctcga catgtccacg gaagacctcg tccagctctt ccccgcgcgc 240 gccaggagaa ggttccagag gggtctgaag aggaagccca tggcactcat caagaagctg 300 cgcagggcga aaatggatgc tcctgctgga gagaagccag agccagtcaa gacacatctc 360 cgtaacatga tcattgtccc tgagatgatt gggagcattg ttggggtcta caacggcaag 420 acatt 425 // ID DY239026; SV 1; linear; mRNA; EST; PLN; 623 BP. XX AC DY239026; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E11.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-623 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 6fb09251deb072183a60c6020f59e5a3. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 11. XX FH Key Location/Qualifiers FH FT source 1..623 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 623 BP; 131 A; 188 C; 216 G; 88 T; 0 other; cgggaccggc ggcttcgggc actggtagca cttggcgaac agcccgaacg tgtcgatctg 60 cctcacgaag ttgaccatgc tggcccagcc gcccagcccg aagccgacca ccagcaccca 120 caccacgatg aacatgttga ggacgaacat ccccgtccag ctcggcagga agaagggcgg 180 cttctccgcg gcgttcatgc gcgcggacgc cgtgcggtag gtgaggacgt gcgccagggc 240 cgggatgatg tagacggtga agctgacgag cagcgcgccg acggcggagt tgatgggccc 300 gaagaagggg aagatgatgg cgaggaacca gatgggcacg acgatgggca gcctcgccag 360 cgcgcgcttg aagatgctct tggcgtcgtg catcccgatc accttctccc acacgaagta 420 gagcggcgtg cacgcgaagc cgaaggtgat gaactggtgg atcagcatca ggatcaccgc 480 cgcgtcgcgc caccgggtct tgggcagcag cgagaaggcg ttcgagtggg tcagcagctc 540 gtcgccgaac gcccagtaca tggccgccga cgacggcagc gtcagcgtga acacgtacag 600 cgtcgccagc aggtagatgt att 623 // ID DY239027; SV 1; linear; mRNA; EST; PLN; 463 BP. XX AC DY239027; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E11.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-463 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 812b836c67b0209102f4cbf8f8f021e2. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 11. XX FH Key Location/Qualifiers FH FT source 1..463 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 463 BP; 84 A; 150 C; 151 G; 78 T; 0 other; ggtccccagc cgtaggtctg ctgtgcaact ccggctcgcc gcctgccaac cgggcggggc 60 ggccaccacg ctaggcccct cgccctcctc ggaggtgaac agagcctgca gtcgatacaa 120 cagtaaattc cgcggttttc ctttcgtttt ccctctcgaa acgcaccctg cttttagtgc 180 acgtcgctcg ccctccggcc atccgcgatc ttcaaatctt ccactgtaaa aagcgggcgg 240 agacgggaag gccgtgcgct caaggatggc gcgcgagcag ctggaggagt ccatcgtggc 300 cgacggcaac ggcaaggagg aagaggtcgg ggtcatgggt atcggcgccg cggacggcgc 360 cgaagaccag cacggcggcg gaaagctcaa catgaagagc ctgctgtggc acggcggctc 420 cgtctgggac gcgtggttca tctgcgcctc caaccaggtg gcg 463 // ID DY239028; SV 1; linear; mRNA; EST; PLN; 348 BP. XX AC DY239028; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E12.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-348 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 50e4902237010e563cbdee1e0795d176. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 12. XX FH Key Location/Qualifiers FH FT source 1..348 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 348 BP; 67 A; 115 C; 95 G; 71 T; 0 other; aaaccgcgcg aaaaaccagg ttgaagtagc aaagatgggg aggtgccggt ccggcgaact 60 ctgcgttatt gatgggagct gagggagtag tacacacggc tgcttgtttt ccggccgaca 120 cccttctcac tatctacata ccagccgaga caggttcact tggcttccat ttcattcacg 180 cgcgatcgag gccacgtgcc cacgtcgcat cctcttccct ctagctagga catcttggcg 240 tgctgcgcgc gctgcctctc cacgtactcg aagaggctgc cgtcgtacac gcgccgcatg 300 gcctcccgcg acaggtaccc ctcgtcgtcc cgcgccagca cgtacagg 348 // ID DY239029; SV 1; linear; mRNA; EST; PLN; 478 BP. XX AC DY239029; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E12.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-478 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; fb6307653b4469a04da8bbc7ba459d80. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 12. XX FH Key Location/Qualifiers FH FT source 1..478 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 478 BP; 97 A; 178 C; 157 G; 46 T; 0 other; ctcacttcat tctgacgagg tgcactccta ggcaacaaac tccaacgccc gcggtagtgg 60 agccaagatg cagggagggg cgacggccaa gcagcaggcg cgagacggtg accgcggcgc 120 ggcaaaaacc ccgacaacgg aggggaaggg cgaccccccc ggccccaggg ctagcgccga 180 cgtggcaaag ggcgaccccc ccgtccccaa ggctgctggg gacgacgtcc tcgtagggaa 240 gggcgacgca ggcaacaagg ccggcgccgg ggccatgcac cacggcctgt cggccgtgga 300 ggcaaaggac tcccagacca tcgtcgcgct gcactccccg gtgaccgtca tgccccccgt 360 ccgcggcgac ctcgaggagc ccctccccaa gccatatctg gcgcgagccc tggtggcgcc 420 ggatatctac caccccgacg gcaccgcggc cgaagagcac ggccaccacc acatgagc 478 // ID DY239030; SV 1; linear; mRNA; EST; PLN; 476 BP. XX AC DY239030; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E14.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-476 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; f8944affa99514ec1cf60c8052a7f409. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 14. XX FH Key Location/Qualifiers FH FT source 1..476 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 476 BP; 121 A; 150 C; 103 G; 102 T; 0 other; gaccagaaag actgttcttt agtttcatta gctgtgtggt ttgcaggtaa gtacagcaca 60 gatctggaca tctgcatcag ggctggatca ttgcacaatg ttaccacaac aacaactgcc 120 catggcggcg gacgtccgcg tcgtcgccca aatgcatgtg catcacatgc ggagcgctga 180 atgacacggt cacacgccca ggctctctca gatctcgttt ccagtactct gcatcccttc 240 aacacagacc agaggaatgc atcgcagcac ggcgtctcac gccagctgca tctgcatcac 300 aatttctagt tcccacagac taaaacccag ctgcttacca tccggtaata cgagacacaa 360 tgacagtgaa acctaatcat cagaatccga cgtcaggtcc atttccgggt aaaacgggcg 420 caacatcttc gccaggtgct cagccatgtc ctcgacctcc tcccctcccg aagccc 476 // ID DY239031; SV 1; linear; mRNA; EST; PLN; 518 BP. XX AC DY239031; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E14.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-518 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 86acaf629856d51e82de595a167516dc. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 14. XX FH Key Location/Qualifiers FH FT source 1..518 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 518 BP; 99 A; 178 C; 141 G; 100 T; 0 other; cggcccactc cttacccccg tctcacaaaa cgccgccgcc gccgtcaaca gccaactccc 60 cgcgccccca ccgcctccgc gcaccgcccc acacctgatt gcgcgtccag aggtattcag 120 agctctctgc tgtgggagca acgagggcag ggatgaggga ggtggtgacg gtgcaggtcg 180 gcggtttcgc gaacttcgtc ggctcccact tttggaactt ccaggatgag ctgctcgggc 240 tcgccgacga ccccggcgcc gacccggtat tcaggaccgc cgcgctcgac atggacgtcc 300 tgtaccgcgc cggcgagaca caccagagcg tccccagcta ctgccctcgc ttggtatcag 360 tcggttctcg agggtctctt ggctcgttaa gttcctctgg cactctaggt tcgagcagcg 420 cagctgcaga tgagcccaac ctcttcacat ggtctggaaa ggtaacaaaa tcagtagcaa 480 aacctcgtga gcgaaacttg ttcctacaaa gtttatct 518 // ID DY239032; SV 1; linear; mRNA; EST; PLN; 229 BP. XX AC DY239032; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E15.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-229 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 25a42d6dd0635b3bfc57d06325d0ff8b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 15. XX FH Key Location/Qualifiers FH FT source 1..229 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 229 BP; 77 A; 61 C; 43 G; 48 T; 0 other; atccaaagta aacatcttta ggcataatgc aaaatcctga cagcgaccac aaaaactgag 60 acaatgtttc accatagtac gaggcaacaa cacatccgtt ctgaaacaca tgtcctttaa 120 caatctaaag cacagtagac agataaacaa cgacaataac tctaggtggc cttgtcagcc 180 ttggaggctg gggcagcagc ttgacccctg agccttccag tcctcctag 229 // ID DY239033; SV 1; linear; mRNA; EST; PLN; 497 BP. XX AC DY239033; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E15.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-497 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 268dcae128cc1f16fcd5505ef17ba4f3. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 15. XX FH Key Location/Qualifiers FH FT source 1..497 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 497 BP; 75 A; 189 C; 143 G; 90 T; 0 other; cgcactgtgc tcttttctgc atccccctcc cgccgcgccg cctcaccgcc gccggagaag 60 atgggtcgcg tgatccgcgc gcagcgtaag ggtgcggggt ccgtgttcaa gtcccacacc 120 caccaccgca ggggccccgc ccgtttccgc tccctcgatt tcggagagcg caacgggtac 180 ctcaggggcg tcgtccccga cgtcatccac gaccccgggc gtggcgcgcc gctcgccaag 240 gtcattttcc gccacccttt cgggtacaag caccaaaagg agctctttgt cgcacccgag 300 ggtatgtaca cgggccagtt cgtctactgc ggtcgccgcg ccaccctctc catcggcaac 360 gtgctgcccc tccgcgggat ccctgagggt gccgtcgtct gcaacgtcga gcaccacgtc 420 ggtgaccgcg gtgtcttcgc cagggtctct ggggactacg ccattgtgat cagccacaac 480 cctgacaacg gcacatc 497 // ID DY239034; SV 1; linear; mRNA; EST; PLN; 380 BP. XX AC DY239034; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E16.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-380 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 4702bfd57ab22516c92eec2a65e1f10b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 16. XX FH Key Location/Qualifiers FH FT source 1..380 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 380 BP; 68 A; 138 C; 118 G; 56 T; 0 other; ttggcattgt cactcgggtt cttctttgtt ctcgtcgtcg tggtcaccat agagggcgtc 60 tgcaagcgct cccaccccaa aaccccgcgg acgcaccgac gcgcgagcca taatgcgccg 120 ccaaagcctc gccaccgtag tgctgctcct gggggccgcc gcggccctgg ccgcccccgc 180 cgctgggcac tttgagtcct cctgcccctt ctacgaccac gggggccagg gagaaccaca 240 caaacgccac gaccatggcc acagctgcgg ggggggcgcg gaccatgagc accaccatca 300 ccatcaccac ggccatggac acggagagat ccagcggctg ctcccggagg agatggggga 360 ggaggcggat ctcgagctcg 380 // ID DY239035; SV 1; linear; mRNA; EST; PLN; 650 BP. XX AC DY239035; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E17.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-650 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 9106e412524db68f339966cd1a00d0a4. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 17. XX FH Key Location/Qualifiers FH FT source 1..650 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 650 BP; 208 A; 158 C; 115 G; 169 T; 0 other; agtaaaaatg agcaccaaat catggatgga atgatgttta ctacaaattc ttacctctga 60 tgtgagctaa ttatgggcaa tgctgtcaat ctgaaactat ggctaaaaat aaactaatga 120 tattgcaggc atgatatcat gccacttttc tagcatcgag acaggtcaat atccaagagt 180 atgtgaatcc atcattaccg aatactagcc aaaatgtaaa gcattgctct tatcagtgcg 240 ccaaaacaac atatccatca acaaacaaac gtaaagcatt gctcttagca gcaactaagc 300 atttgaacgt caactccacg atcggccacc cccatacccc ctatatctat cacggtaacc 360 tgaagatgga ggaggtgcac cacgacccat agcagctaat tcagagctga ccttttgtcc 420 agcttcttcc agaatattaa taagttcctt ggcaaatctg gcattagctg cagtgaaaaa 480 ggtgtacgct gttccttttg cacctgctct gccagttcga cctatacgat gaacataatc 540 ctcgagtgac cccggaaagt catagttaat gacatacttc acatccttga catctaagcc 600 tcgagcaaca acatcagtgg ccgtcataat aggacttttc cctgacttaa 650 // ID DY239036; SV 1; linear; mRNA; EST; PLN; 483 BP. XX AC DY239036; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E17.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-483 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; b7e070a9a69082a50998b838b5ef8994. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 17. XX FH Key Location/Qualifiers FH FT source 1..483 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 483 BP; 88 A; 168 C; 146 G; 81 T; 0 other; caccgatttc actggcttct tttcgaatca taaatttcat ctcctggagt gttgctttgc 60 ttgctcctgg aatcttcgga accgaaggcc accgcgccaa ccgcccaatt accaaaccaa 120 accctaccgg tcgcggctcc tccatagtcc atactctcgc gcgcctaaac cctacccatc 180 ctcctcgccg ccggagaccg ccgccatgag ccgctacgac ggccgcgctg cggacgcggg 240 ctcctaccgc gaccgccgca gcgagggggc gttcggaggc gggtcgaggg cgttctcggc 300 gccgagcaag gcggacgctt ccgcggcggc ctcagagcta gatgggctgc cgcgcttcga 360 gaaaaacttc tacgtggagt cacccgcggt ggccggcatg acagaagaag aggtggaggc 420 gtaccgccgc cgccgggaga tcaccgtcga cggccgcgac gtgcccaagc cggtgcgaga 480 gtt 483 // ID DY239037; SV 1; linear; mRNA; EST; PLN; 488 BP. XX AC DY239037; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E18.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-488 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 6dfee5526fbd3dc3f9d43f799cd406dc. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 18. XX FH Key Location/Qualifiers FH FT source 1..488 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 488 BP; 153 A; 99 C; 110 G; 126 T; 0 other; gcatttgtgc agaaaaggca cctgagagat caaagctcta gaatccctcc agatcgagaa 60 ggctatgcat tggatcccag tacaagattc aaatacaacc aaagatagat tgagagccac 120 aaacaagaga gagagagaga gagagagaga gaagcatgta cggatggcca atctcaattt 180 tcaaccctgc tctttgcatt tgtcgttcat tgatcccaac taaaatttgt gatggctgac 240 gggggaaagt tggcagctag caatctctat ttgcacgggg ggaacaactg ccctgggctt 300 ctatagtttc ataagatgtc ctctttgttt ctgagatcat tgcactagtg gcagaccggt 360 ttggtacata acaagaaaat ctgaagctat ttaaaagaac tcatttgacc gccaatggtt 420 agtgggttca tgcaactctt gtacccttca tcttgatctc actagcagaa gaatttaaac 480 gtataaac 488 // ID DY239038; SV 1; linear; mRNA; EST; PLN; 386 BP. XX AC DY239038; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E18.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-386 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 83824b15403f9594a3ec160eb5300d22. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 18. XX FH Key Location/Qualifiers FH FT source 1..386 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 386 BP; 53 A; 142 C; 129 G; 62 T; 0 other; gcgccgtccc acccttgctc ccactcgttg cgccgcgccg cgcgatgcag gcccgcgtcg 60 tcttcttccc cgtcaagggc cgcgcgcggg tgtttcgccc tcccgcgcgc ctccgccgca 120 gcgcccgctg ctgatggcgc cctcccgcgc cagaccctga gggacctctg gcgcggtatc 180 tcctccgggt cccgcacggc gccagaaaag gccgaggcgg tcgtcgactt cctcgctgac 240 aagatgaaca gggcgtgggt cgggttcggg agcgcgccag aggggtcgat aaagagccag 300 atccacagct tcgggctgaa tctgctgtcc cgggagaggc cgtcagaggt gctcctgaag 360 tccgtcacca aggacgtgag cgcgct 386 // ID DY239039; SV 1; linear; mRNA; EST; PLN; 293 BP. XX AC DY239039; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E19.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-293 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 6d5716db339884f060749c3d621643b0. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 19. XX FH Key Location/Qualifiers FH FT source 1..293 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 293 BP; 70 A; 83 C; 71 G; 69 T; 0 other; tgagataata gtgtttgcag tgcttcgaat gggagtaaat atatcgacat ctcaaatttg 60 ctgcacatct ggggcgcgag accacaacac gttagttaac ttggttcacg ggttggggac 120 gagctccttc acaaacgcga ccaccttggg cacatcgacc gtccatgcga ccgaaatcgg 180 tgagtagcca gtccacgggt tctctgagtt ccacttcttc agcaacatgt ccatggaagt 240 atgcccgaag cagatgccct gcgtctccac cctcaccacg cccttcttga acg 293 // ID DY239040; SV 1; linear; mRNA; EST; PLN; 349 BP. XX AC DY239040; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E19.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-349 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 589447400fbc7e3a90e3d0051f8f232d. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 19. XX FH Key Location/Qualifiers FH FT source 1..349 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 349 BP; 85 A; 72 C; 105 G; 87 T; 0 other; cgttcaagaa gggggtgggg agggtggaga cgcagggcat ctgcgtcggg catacttcca 60 tggacatgtt gctgaagagg tggaactcag agaacccggg gagtggctac tcaccgattt 120 cggtcgcatg gacggtcgat gtgcccaggg gggtcgcgtt tgtgaaggag ctcgtcacca 180 acccgtgaac caagttaact aacgtgttgg ggtctcgcgc accagatgtg cagcaaattt 240 gagatgtcga tatatttact cactttcaaa gcactgcaaa cactattatc tcaatagttg 300 gttcctgtgg aggattgcaa atcattttga gatgttttaa attgcagca 349 // ID DY239041; SV 1; linear; mRNA; EST; PLN; 482 BP. XX AC DY239041; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E20.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-482 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 99976be2852ae13f6e883ff937ee03bd. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 20. XX FH Key Location/Qualifiers FH FT source 1..482 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 482 BP; 145 A; 128 C; 97 G; 112 T; 0 other; aaaaacatag attttatcta gattgccaaa caacattttg tatataatac cgtagtattc 60 gagaatacta cagtattcta ccaaaactgt gaaaaaaaac ttgactccca gacacccccg 120 aagctagtac aaacttcaaa ctagtcgggc ccaaagggaa gtcgcagtcc tccgttttat 180 cttcgtccca tggagggagg ttttcttcag acgttaaagt aaccaattta tttggcaact 240 ggagacacca ctgaaggtac aactacagtc gccttattgc gtcctctccg ccttcactca 300 tcatgttggt cgatctctag aacccataga catgcatctc atgaacgaag tagtacgagc 360 aagcctgcag agccgaagta gtacgagcaa gcctgcagag cgggcacaca gccgcatcca 420 ggctcgctac tgagaaccca gtctctggac atctaccagc tgatagagat cgccacgccg 480 ct 482 // ID DY239042; SV 1; linear; mRNA; EST; PLN; 444 BP. XX AC DY239042; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E20.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-444 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; b08d4e1c89859ce7c05bff31272bc629. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 20. XX FH Key Location/Qualifiers FH FT source 1..444 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 444 BP; 89 A; 157 C; 122 G; 76 T; 0 other; ccacagccgc cgcgctcctc gcccccggac taaagttctg ggggggcggg gggggcgtct 60 cctctccctc cggcctccca ccccgccgcg tcactgcgat ggcctccgcc cccaactcct 120 ctttccgccc agaggaggcg cgctaccctc ccgccctcga gctccagatc ccgcggctct 180 ccaagttcaa gggggcgctg tgccagctct cggagacggg ggacaagagt cgcaacatcg 240 cgcacgcgcg agcggccatc gaaaaggcag cctccgacgg cgccaagctc gtggttctcc 300 ccgagatatg aaatggccct tactcaaatg acagctttcc agaatacgca gaggacattg 360 aagctggcgg agatgcaccc ccttcatttt caatgctgtc agagatggcc agctgaaggg 420 aaaacataaa aagatccatc tttt 444 // ID DY239043; SV 1; linear; mRNA; EST; PLN; 470 BP. XX AC DY239043; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E21.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-470 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; f150641ec1c0ce1f850c84426c79a6d4. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 21. XX FH Key Location/Qualifiers FH FT source 1..470 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 470 BP; 122 A; 118 C; 90 G; 140 T; 0 other; gttatcaaga gcagggtttc cacaatatag caagtaatta gttctgacgt aacacccact 60 tgtccaaacc acaacaattg tgatagctca agcacaagga gagacttgct ggcaaagaat 120 aaaaattatg aaactatcct gcggccacaa ccgttcaaga gccgcagcaa gctgatttct 180 gcgcctctgc tgcatcagct tccccttcag ccttgatctt gatagttcga tcctcaggct 240 tggaatcagt ctctgaaagt ctctgtttga tatcccttgc tatagaaaag aaaacctgct 300 cgacgttcag gtttgtcttt gcgctcgttt caaagaactt tatcccatat tcgtctgcga 360 gagcttgtcc ctttgaagtg ggcacagccc gtttgctttc atccatatca gctttattcc 420 ccaccagaat tttgttcaca ttatccgaag catgttgttc aatgtttctg 470 // ID DY239044; SV 1; linear; mRNA; EST; PLN; 309 BP. XX AC DY239044; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E21.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-309 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 994d27538c27c4c2c51363ecaeb14237. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 21. XX FH Key Location/Qualifiers FH FT source 1..309 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 309 BP; 54 A; 101 C; 92 G; 62 T; 0 other; gggccgagca acctggtgct ccccccctcg ccgttacagc ggcgcctcgg tctccggttc 60 cacagcgtct gcctgcctgc gcggccctgt gaccgtgagg cgacacgacg ccgagagatc 120 acccgccccc cgcatcgagt cggccccgtt tgggattgtg aggtaaagcg tgatgggagc 180 gccgccggcg agggctcgag ccgactacga ctacctaatc aaagtgctcc tcatcggaga 240 caggggtgtt ggaaaaagtt gcctcctgtt acggttttca gatggatcat tcaccactag 300 cttctttac 309 // ID DY239045; SV 1; linear; mRNA; EST; PLN; 443 BP. XX AC DY239045; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E22.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-443 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; ae0f6323f1dba1990b559dea5eb8063f. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 22. XX FH Key Location/Qualifiers FH FT source 1..443 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 443 BP; 65 A; 159 C; 147 G; 72 T; 0 other; gccatcgaca cgatcgcgag cgcaagcacg tacgttgtcg gaagctcaag ttagaagaac 60 caccagtgcc tgatcgatgg cggcccaggc tctcctctcc ggccggcagc tgctgggccg 120 ccccgtgcag tcggccgtct ctgggtcgtc ggcgaggagg gcgcccttcg tcgtcggggc 180 cagctccacc cctccaccca agcagggtgc cgacgggcag ctgtggttcg cgtccaagca 240 gtccctctcc tacctcgatg gcacgctgcc cggcgactat ggcttcgacc cgctgggcct 300 ctcagacccg gagggcaccg ggggcttcat cgagcccaag tggctggcct atggcgaggt 360 gatcaacggc cgctacgccg tgctgggcgc ggtgggcgcc ctcgccccgg agatcttcgg 420 caagatgggc atcatccccc cgg 443 // ID DY239046; SV 1; linear; mRNA; EST; PLN; 358 BP. XX AC DY239046; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E23.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-358 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 90592d834fa970595c393539b3e17678. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 23. XX FH Key Location/Qualifiers FH FT source 1..358 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 358 BP; 36 A; 163 C; 118 G; 41 T; 0 other; ctgctccagc cgcatggtgc gcatcttggc ctcgaactcc tcccacacct cgcgccgccg 60 catctcgcgc tccatctcca gctgctgctc cacgcgcttc agccgccacg cagcctcccg 120 ccggtgcgcc gcccacgcgc gccgcccctc ctccagctcg cggcagctcg tcggcgagct 180 ccgccagcgc ctgcccgtcc acgaccgcca tgcccggggg cgcggcgccg ccgtgctgcg 240 acgggcacgg gaacccgtac ggcgccgcgt cggccgcggc gcgatccggc atccatggcg 300 cgggcgcggg gcgggcccgg cgccccccgc gccggacgcg aggctgagcg tcacggaa 358 // ID DY239047; SV 1; linear; mRNA; EST; PLN; 273 BP. XX AC DY239047; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E24.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-273 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; b7c9a7761ea190e420c9a5fe989e726e. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 24. XX FH Key Location/Qualifiers FH FT source 1..273 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 273 BP; 52 A; 98 C; 66 G; 57 T; 0 other; attattaatt cgttaataac cacctttaat gaagttatca aaatcatcac atagcgaggt 60 taccctcctc atgatcctgc aaaacccagc gtccgctggc gtccctggtc atgcccttgt 120 tcccctccac ccaccaggcc gccggcacgg ggtagtcgtc gctgagcgcg tccacctcct 180 tgttcaccag cgcgacgtcg cggtccacct ccaggctgaa cccggcgggg tcgccggccc 240 cctgcgtgcc ggcaacaccg tggaggtagc act 273 // ID DY239048; SV 1; linear; mRNA; EST; PLN; 534 BP. XX AC DY239048; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257E24.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-534 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 2e59fdcb077987392463e1e835f137e8. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: E column: 24. XX FH Key Location/Qualifiers FH FT source 1..534 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 534 BP; 83 A; 184 C; 197 G; 70 T; 0 other; cgttcagttg caattgcaag cagcacaagc tagtctccaa gatgcaccgc tccttcctcc 60 tcggctgtgc actcgtagcc accgcgcttt ccaccgacac caccgttgcc gccgcccaga 120 gccagagcca gagccagagc cagaggtcgg gggccgaact cagcgggggg gacaactggg 180 aggggctcgt ggacccgctc gagggagacc tcgggcgcgc cgtcatccgg tacggggagc 240 tggcgcagga gacgtcagac gcgttcatcg gagacccggc gtcgccgtac gcgggcgcgt 300 cgcggtacgc ccccggcgcg ttcctccgca gggcgcaggc gcccgacccg gacgcgtaca 360 acgtgacgag gttcctgtac gcgacgtcga gcgcgcgcgt ccccggccag ttcatcacgc 420 ggccggcgcc gccgggggcg tggagcgccg agtccaactg gatggggtac gtcgccgtgg 480 ccacggacgc cggcgtcgcg aggctggaga ggcgggacat cgtggtggcg tggc 534 // ID DY239049; SV 1; linear; mRNA; EST; PLN; 577 BP. XX AC DY239049; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F01.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-577 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 3d63adc843db035df0c415c343bb8296. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 01. XX FH Key Location/Qualifiers FH FT source 1..577 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 577 BP; 132 A; 168 C; 128 G; 149 T; 0 other; cgcaacatga cttatgacac tcagatatgg tactcgtgtt cagccacaga tgctgcaacc 60 agaatagtag ctcaagctaa ttacagtagc cagtagccaa gaatcgtcac caacctctca 120 tcagcataag ttgcttttgg accttcagtc catagctaga taatataaag gaacactcgt 180 agaattaatg catggttaaa gcccccatct atagcttttc ggacattctc ttcagcactc 240 gtgctgccac tgtttcctcc tgttcgtcat tctgatcctt ggtgagccac tcttgagtgg 300 cgacaaggtc cgggagcacc gtggcgcctg actgcctcca gctctcagcg tcggcagtct 360 tgaaccggtc caggagcagc gcatgcatcc cgatgctcct agctggggtg tagtccttcc 420 gcatgctgtc acctatgtgg agcgcttctt ccggagcgac gtttcctgcc atctccagcg 480 ctatcttgta aattcttggg tctggcttct caacgccaac gacacctgag aacaccccaa 540 agtcccattc ggagctctga ttcaatccta aagctgg 577 // ID DY239050; SV 1; linear; mRNA; EST; PLN; 547 BP. XX AC DY239050; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F01.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-547 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 9ce4301abb662b31538bbe5a4177ecc8. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 01. XX FH Key Location/Qualifiers FH FT source 1..547 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 547 BP; 143 A; 125 C; 164 G; 115 T; 0 other; cgaaaaggga ctcgctgttg gcatcgtcag caatgaataa taccgctaca aagatgtaat 60 cctgccagct ttaggattga atcagagctc cgagagggga ctttggggtg ttctcgggtg 120 tcgttggcgt tgaaaaccca gacccaaaat tttacaagat agcgctggag atggcaggaa 180 acgtctctcc ggaaaaagcg ctccacatag gtgacagcgt gcggagggac taccccccag 240 ctaggagcat cgggatgcgt gcgctgctcc tggaccggtt caagactgcc gacgctgaga 300 gctggaggca gtcgggcgcc acggtgctcc cggaccttgt cgccactcaa gagtggctca 360 ccagggatca gaatgacgaa caggaggaaa cagtggcagc acgagtgctg aagagaatgt 420 ccgaaaagct atagatgggg gctttaaccg tgcattaatt ctacgagtgt tcctttatat 480 tatctagcta tggactgaag gtccaaaagc aacttatgct gatgagaggt tggagacgat 540 tcttggc 547 // ID DY239051; SV 1; linear; mRNA; EST; PLN; 224 BP. XX AC DY239051; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F02.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-224 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 0c1994b77f7db98c47df5d7a165f77c9. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 02. XX FH Key Location/Qualifiers FH FT source 1..224 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 224 BP; 92 A; 54 C; 37 G; 41 T; 0 other; accaccattt tcaattcgca ttcaaccaaa tcagaagagc ggtagtagta caaaactcga 60 atttacagtc gagtgaaaat gaagcagaaa aaaaaagaaa aaaacagtca atcgattaga 120 caaccacccc atcgccgccg tcgtctccaa aaaaagaaac aaaatcagtt aaaccaacga 180 gcagccaaag ctatcctctc atcatgattc atgacggtcg atgg 224 // ID DY239052; SV 1; linear; mRNA; EST; PLN; 500 BP. XX AC DY239052; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F02.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-500 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; df7df7ea1fa057494549fb9cddf9b723. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 02. XX FH Key Location/Qualifiers FH FT source 1..500 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 500 BP; 85 A; 208 C; 142 G; 65 T; 0 other; cggctccccg cccaccgccc gccactggat taactaccgc ccgtaccggc agctccagca 60 agagtgccac tcacacacac gtcacactgc gtagtgcgca gggctccgac tttgatgcgc 120 gtacccgcca cggtccatgg cctccgccgt cgccagaaac ctccccgtcg ccacgccagc 180 cgccgtgccg gtgccttacg ggaggctcac cccgcgcgtg tcaatcaccc gcgacgcgcc 240 cgccgacctg ggggatcccg tcgccatggc agtggccacg ccggaccccg cgatctccaa 300 ggacttcatt gacttcgagt tcagcctcgg cagctccgcc accatgctcc ccgccgaaga 360 gctgttcgcc gacggcaagc tgctaccgct gagaaaggcg gcgactctgc cggatccgga 420 ggccgccgcg ccacccccgg cgcagccaga ggaggcaatg ccggcgacca gcacgagagc 480 cgatcaagcc tctccgcgcc 500 // ID DY239053; SV 1; linear; mRNA; EST; PLN; 330 BP. XX AC DY239053; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F03.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-330 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; aa1db0a3dd319b89b44fa3afbd21c066. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 03. XX FH Key Location/Qualifiers FH FT source 1..330 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 330 BP; 46 A; 100 C; 110 G; 74 T; 0 other; cccttggtcc aggcgcaggc gcgagcgcga cgtccggcgt ccggcttcgg cggaggagtg 60 gagcgatccc gatccccaca aggaattcca tcgctgtcgt ggcgttgggg ggcgctcctc 120 ccggccggca agcaggatca ggtaccctcc cgtcccggat cctcccaacc gttccgtttc 180 ccgcggccgg ggtggtttat ttctgcggcg ctggttgcca tttcgggcca aatttgttgc 240 gtgaagcgga aggatttgga ttgaatggca cgcgttgttt ccagtcgacg ccctcttggc 300 ttgcctttgg ctgtaggaaa tcgcacggag 330 // ID DY239054; SV 1; linear; mRNA; EST; PLN; 443 BP. XX AC DY239054; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F05.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-443 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; a2cd2f47440376066bc437b74407de0a. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 05. XX FH Key Location/Qualifiers FH FT source 1..443 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 443 BP; 115 A; 135 C; 93 G; 100 T; 0 other; gcagcaaaac ataatgactc aggttaaaca ctcggaacat ggcctctcct cccttgatgc 60 catgccatgg gtttcgacat ccaggatcca gataatgcag aaaccgagca gctcacgata 120 aaataaaaaa cgacaagact cgttgccaaa aaaaagaatc cgtttaccct cgttatacac 180 aggacagatc atattcacaa gctacagtct acctacccaa accacctgac caagcaacgc 240 tttccctttc ctcgctaacg agaacgggcc aataatggtt ttggatttgg accaacagtc 300 gtctccttcg tccctcccag ggttctccgg cgaagcttgc gactgctctt gctagcgctc 360 gcggcagcag cagcaggcgc ctgtttggtt ccagccttgg gtccttgacg cttctcggcc 420 tcaccagagt cttggagctc ctc 443 // ID DY239055; SV 1; linear; mRNA; EST; PLN; 524 BP. XX AC DY239055; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F05.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-524 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; fca9a0ccd5bed7cb75aea491dc75c07b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 05. XX FH Key Location/Qualifiers FH FT source 1..524 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 524 BP; 98 A; 151 C; 176 G; 99 T; 0 other; aagcggcagc ctcaggcgct ccaccacgcc accaccctcc ttcctctcaa tatactcact 60 cctcccgttt tccgtccatt ttcatccatc cgcccacagt ttcgcagccc gcgcccctaa 120 aaccctagcc cggggggatc ggggggcgcc tcgggtgcca gagacgcggc tcgtttgatc 180 cgaggggggg ggcgggcccc gccctcggtg ccgcgcttcg ctccgggggt ggggggggga 240 gagcaccgag cacgcgggaa gggggaagtg gtggggcgcg cggatcaggg aggggagcgc 300 tggcgcctca ggaatgttct caaagagggg gggaaaggat gggagtaggc ttagcgggtg 360 tagtagtcga gcctcttcag cttccgtgcc tccaagtgag aagactgagt gagaaatcct 420 gcaatccgcc agtgttaaga tcttcacctt caataatctc aaggcagcca caaggaattt 480 ccgtcgtgat agtgttctag gagaaggagg gttcggctct gtct 524 // ID DY239056; SV 1; linear; mRNA; EST; PLN; 458 BP. XX AC DY239056; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F06.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-458 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 91f4425712185dbf589b07336828fc69. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 06. XX FH Key Location/Qualifiers FH FT source 1..458 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 458 BP; 117 A; 144 C; 76 G; 121 T; 0 other; cccaacttca taaattaggg agggtacaca ttcatcaccc atgttttatt tccgacaatc 60 aaatcccaac gagcatagca ccgaccgaac tgacacatcg catgcataat gtgttcatcc 120 ctctctagga gggcaacacc gttctacaac taaaaaaccc aaaccaacta caaatccacc 180 gacccagagt tgtcagttga cccattatta ttcctccgcc tgatcaacat tacacacttg 240 tttccttcat tcatctgtcc gttcttcttt ttaggcctct tcctcaagca gcatctgatc 300 gaagtgccac accccgtgcc ccttgcccac tctcctcagc tgggcgacat acttggaaat 360 cttgtttatg gcttccacct gctcctcgag gaactcactc tcaaagaaat ctggcagctg 420 aggatcattg ccccttgttg ccacaccatg gaggttgg 458 // ID DY239057; SV 1; linear; mRNA; EST; PLN; 522 BP. XX AC DY239057; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F06.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-522 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 6adab029cd2dfd28e89a8b24728f9528. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 06. XX FH Key Location/Qualifiers FH FT source 1..522 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 522 BP; 96 A; 191 C; 150 G; 85 T; 0 other; catccatcct tctattctcc agtccaccca ttccattcca ctacccacgc gaagaattca 60 cgcgaacgcc cacgcaccca cacgacacat ccagatgatg cttagggttt ccccgtcccc 120 ggccgccgcg gtgccaaccc agctctccgg tgcgcccgcg acccctgctc ccgtcgtcgg 180 ggggggggcg ccgcgcggcg tcccctcgcc ggccgctggc gccgcgtgcg gggccgcggg 240 caaggggaag gaggtgctca gcggggtcgt gttccacccc ttcgaggaga tcagggggga 300 gctcgccctc gtgccccact ccccagacaa gtcgctcgcg cgccacaagt tcgtcgatga 360 gtgcgaggcc gccctcaacg agcagatcaa cgtggagtac aacgcctcgt atgcatacca 420 ctccctcttc gcctacttcg accgagacaa cgtggctctc aaaggatttg ccaagttctt 480 cagggaatcc agcgacgagg agagggagca cgctgaaaag ct 522 // ID DY239058; SV 1; linear; mRNA; EST; PLN; 336 BP. XX AC DY239058; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F07.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-336 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 210d792ed511407872b9bd41bff42d10. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 07. XX FH Key Location/Qualifiers FH FT source 1..336 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 336 BP; 113 A; 91 C; 61 G; 71 T; 0 other; gaaaaaaaaa aacaacacat gtgcattgaa cctacaggct atatacagaa caaaagaaaa 60 tgtgtacaat tgtctgaagc ttttggcacg tgaacgcgcc tgatcagcat ctgcaatgga 120 gcgcctttct cctgtaacca tgcactagtg ttctacaaaa acctcaccag ctaggcttca 180 aacacacgaa ggcactgaca gctactacct gatcctagaa attaacagct taattacaat 240 cggagaacgc ttcccaaact atactacacg tccagctgtg ttatctcaaa gtaagaggcc 300 gaacgccctg aaaccgacga cctgcccgag ctctcc 336 // ID DY239059; SV 1; linear; mRNA; EST; PLN; 403 BP. XX AC DY239059; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F07.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-403 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 72f53412187f345b9e5a2aea6c495daf. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 07. XX FH Key Location/Qualifiers FH FT source 1..403 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 403 BP; 47 A; 136 C; 131 G; 89 T; 0 other; cgcttctcct cccgctgtcc cgcaagaaca cgagcaggag gggtggccgt ggcagccggt 60 ttgacccgcc cccgagcgtt ataagctgtt tctccgggaa cgtcttgtcg cgcaagtcgc 120 tcgcgccggc cgcctccacc tgtttgctcg gtctcatatt cccgcgcgcg aaagcgtgga 180 ccggacagcc ggcaggcgct cttttctttc attccctgtc ggggtctctc gtgctctccg 240 tccgttctaa cttctgcggc gcgcgaaggt actgtgggaa tcgctgctct ttccttgatt 300 ccggcggcgg cggaggtgcg cgggttccgt tcgtcgcgcc gtgggggtgc gagggaggcg 360 ccgcgcgcgc cctcagattc aatcggacac agcgtccgtc gcg 403 // ID DY239060; SV 1; linear; mRNA; EST; PLN; 590 BP. XX AC DY239060; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F09.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-590 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; f8248427b432a42c8661242eaa9fa436. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 09. XX FH Key Location/Qualifiers FH FT source 1..590 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 590 BP; 186 A; 119 C; 96 G; 189 T; 0 other; gcagtaacta aaatattgga tggtgttgaa catacaacaa gttctagaag ccattatcta 60 accacataat cagctgacct taacccagtg accatttaac aacgtatata atataaattt 120 actttagatg ctttattttt gcactgtaga cctattgcac atataagtgc tccttaaaac 180 ttagggtgtt attttcagtc tcattattcc attctttact aaagaattgt acatcaaaca 240 aatttatgta gtacatatat tgcagaggtc ctcaaaatct acaccaagtt atgtcgggga 300 ctatatctcc actcaactca ttttaacctt gtacatttca atcaaatcca aatacatgtg 360 aaataaatgg agtaggttac tctgaccagt gttgcgctcg cgcgccagca tgctcggact 420 aaccaacgaa aaaggtgaag tgggatggta taccatttgc aattgacatt tatggttttt 480 gctacgctgg cactcagtgt ttccaaatat ctattactca gtgtgcgatc gtaccaaatt 540 caacagggct ctctacatat ggtatgtata actatctcaa tttgaactgc 590 // ID DY239061; SV 1; linear; mRNA; EST; PLN; 353 BP. XX AC DY239061; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F10.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-353 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 0767549541ce2dd9a14e6f7c60bb1cdc. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 10. XX FH Key Location/Qualifiers FH FT source 1..353 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 353 BP; 131 A; 70 C; 55 G; 97 T; 0 other; gagatggaaa ctcattttag gacgagaaaa ggaagtacat tacaggtgtg tttaaatgca 60 ctagagctaa tagttagctg ttaaaattag ctaaagacat ccaaacatac tagttaataa 120 ttcaactatt aactattttt agcaagttag ctaatagtta actagctaaa ttcaccagta 180 gttttagcca actatctatt agctctagtg cttttaaaca ctccatacac caaaatgttc 240 ataataactt ttaccacgcg aaagaacaga tgaaacagtt agccaatcca gcccacccaa 300 catcacaact gctcatgatc atgatgcatc tagtgatgaa cgtcgaccga aag 353 // ID DY239062; SV 1; linear; mRNA; EST; PLN; 474 BP. XX AC DY239062; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F10.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-474 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 7adce2c594d79f64f9c14bc5b7ac6901. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 10. XX FH Key Location/Qualifiers FH FT source 1..474 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 474 BP; 86 A; 160 C; 114 G; 114 T; 0 other; cctctccggt ccccactagt ccccttccct tccctcgcct accttcgcac gggtgctctg 60 cccgttcgac ggccacgccg cgccgcccag ctgctccggc acccgccttt cttcgtcgag 120 ctcgggatca gcatctcgct tgttcggggc tccccattcg gcctctcctc gccctaactc 180 tattttgcag catgagacca gcacaacagc ccgaatcata ggcttgacgc agagacccca 240 tctcatctgc gctcccgcgc ggggcctaac gctaacggag ccagaagcaa aggagaggat 300 tccacaagct tcgagttgcc gaaacatttt gttaaatcta ctgcttgtgt gggatcatta 360 gtgcaaatcc ggtgcttgcg cgtgcagggt gtgctaccca tgcgtcttgg ctattgagaa 420 taaaaggttt cgcctgttgc atctgtcact tgcgatatac ccgcgctctg aaat 474 // ID DY239063; SV 1; linear; mRNA; EST; PLN; 378 BP. XX AC DY239063; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F11.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-378 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 0947df7bbb34e26fddfc0e503dcb6e52. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 11. XX FH Key Location/Qualifiers FH FT source 1..378 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 378 BP; 98 A; 112 C; 82 G; 86 T; 0 other; gctcaagaaa agactaagct catttttctt ggtcaacggg ttgtgttcct cagtgttgtt 60 catttcaatg gaaaggggtc aagtcaagag ctgcatttac aagaaagcat agcaagacac 120 cagaattatc ctctactctt aggaacatac cagtccctct tcgtctgatc ataagcaagc 180 gaggaacagc tgccattgcg gtaatcccac gagagcacct tcttcatcag gcctcgcagc 240 ctttcggcac caggtccagg ctcgaaaaca gagtcttcac ccctagacag gcatggatca 300 cctcctcctt cagagctccc gtcacaccat gctccagggg tgaaccaccc ctcccagcgg 360 tgcaagaact cgcggtct 378 // ID DY239064; SV 1; linear; mRNA; EST; PLN; 449 BP. XX AC DY239064; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F11.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-449 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; c93402199a2ae329872d50b95f6eaa12. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 11. XX FH Key Location/Qualifiers FH FT source 1..449 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 449 BP; 64 A; 157 C; 131 G; 97 T; 0 other; gaatagatca ccgccgcccc gcgcgaggtg cttcgctggg gaggagcgga gccctgctga 60 agttggccgg ccgtgcccgg cggcctcacg agctctctcc tctttgctct acgccggggc 120 tgggccttgt cctcgtcgcg cgtacgtgct ccccgccgac gcggcccctg ctctctgccc 180 gtgcggaaca acgggggcct gcgcgcctcg ccgggccact cgctcgtcgc gaacgagagc 240 tcggattgtt taagaggcct catccgctgt cctctctccc ctggcgagca aaatccctct 300 tgaaattccg aggcttctct tgaggatctt gctctccaga gctcatctga gttccagatc 360 cgtttcgtcc ccgccaccaa tttggtgcgc tcgcgagatt ctatatcacc ggagaaaagc 420 cggtgtgctc cgtaaggtcg gacatcctg 449 // ID DY239065; SV 1; linear; mRNA; EST; PLN; 308 BP. XX AC DY239065; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F12.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-308 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 592ebc87ca2e312ac5e80c53cfb44017. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 12. XX FH Key Location/Qualifiers FH FT source 1..308 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 308 BP; 95 A; 69 C; 61 G; 83 T; 0 other; atagaacatc agctaattgt tctaggctat gttttaactt ctcgaacaca acgtgaatag 60 tctatccaca acctccacag acgacaggag accacgcgaa gtaccacccg agtttgaata 120 gttttcagcc tacataagca aaatctttct acaagtcttt tatctaggac gatgttaaaa 180 cttcctgctt tagttgtcaa atagctgaga gatgtataag tgcctacaac ttcagcaaaa 240 ggccaattgg actgtgatcg ctgagcgcta catctggcag agtgtaagaa tcatggacct 300 tgtcggca 308 // ID DY239066; SV 1; linear; mRNA; EST; PLN; 586 BP. XX AC DY239066; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F13.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-586 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; b30e3b5a4618acae4f4725dd34e71135. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 13. XX FH Key Location/Qualifiers FH FT source 1..586 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 586 BP; 174 A; 159 C; 130 G; 123 T; 0 other; gacaataatt gaagacgcat ttcaatttac atccaaatgc atgacgctat ttacagacgc 60 cgacacatgg acccacccaa ccttactact acattttagc agacacatac aaacatctag 120 cactacatac gccaccctat ttacatccga gagctgtgct ctgctccctt gcaccaaaag 180 ccacccacgg tcatcacaag gggcaacaac aagcgctctt actcgactcc gactcaaaga 240 aactaccagg ttttttaacc aaagaaacta gcaggggaaa tgtacatgtt gcgggggtag 300 caaaacaggg ttaggtaggg tcaatgatcc ggatcatcag gcaaaacagt gagggggctc 360 tttgttcccg caagcaaaac cttcgatcct tactttcggg ctgctcgccc cgcaagacga 420 cggagaacct acttcgactc ttccagctgt cattttcccg gaagagcttc caccaaaaaa 480 aggagaaaag gatggggttt tctttcccaa ctgtgggtca ttgaaaacag ggttgtcagg 540 gcgtgcagga ggcgagccac agaaaaagcc cgcccggctg cttgaa 586 // ID DY239067; SV 1; linear; mRNA; EST; PLN; 542 BP. XX AC DY239067; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F13.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-542 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 55599439bdb38933a7392b221832dcda. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 13. XX FH Key Location/Qualifiers FH FT source 1..542 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 542 BP; 108 A; 167 C; 120 G; 147 T; 0 other; cttttctttt tctcattgcg acgcctgcct ggacgaccgt cgccgctcta caccaggtgc 60 ccaggtcttc gcgcggcgcc gagcagccac cagccgctgt gcaacaaatc acgtctgtcg 120 agggcatgga tccctacatg acaaggctcg gcgccggacc cactcaccat ttttccaggg 180 ctgaagcaat gcctgaccga aggtctaggt tttggcagac agatgtgcag cttgcccctc 240 agatagacat catctgccct ctgcctcgcc gcccttctcg cctaccggtt ctcaataccc 300 ccaagctcaa tgggttagtt ctttcttcac tctcctctat ccaccacgta cttacatgca 360 ccgtactagt gttgaattac aaattgctga agctgcctca taggttgcat attcatgtat 420 gcctgctgcc tgcatacttg ctagattttc agcgccaact ggtaggctat atgttgcatg 480 cttgtatgta cttacgttca tcactgctag attgttagcg ccaactggaa ggccacatgt 540 tg 542 // ID DY239068; SV 1; linear; mRNA; EST; PLN; 502 BP. XX AC DY239068; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F14.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-502 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 547705102de1952a5ae66f23923ba412. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 14. XX FH Key Location/Qualifiers FH FT source 1..502 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 502 BP; 119 A; 155 C; 120 G; 108 T; 0 other; agcacagaaa cccttctctc tcttatttta ttcctgaatc cataacagtc tactgagttg 60 acacacagtt cagtccaaag catcaaaagc aaaccgaaac aaagtttctc taccaggacc 120 caagatacca aactgacaaa agaaaaagac accagatcct tctgggttca ttttatttgt 180 ggcaatgcag gctgctgtgc cactccatgc attcactccg ttcagtttat agaggagagg 240 cgctggacga gcatgttgaa cacgttgtcc ctgtccagca tgttgatcct gacgcaccgc 300 gcgtctcctc cgaactgctc cccgccgcta cccacgatct tgtggccggc gagcaactcg 360 gcgcagtcct cgacgccctc cttctcacat cgcagccacg cgaacgcagg gcaagctgtc 420 acggtttgct tggcgaagct gcagtagccc gaggtctcct cgggcaggct gaaggcgccg 480 gtggcagcca cggtggcgcg ca 502 // ID DY239069; SV 1; linear; mRNA; EST; PLN; 400 BP. XX AC DY239069; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F14.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-400 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 58aa5f90792b408b930bc4a68df85736. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 14. XX FH Key Location/Qualifiers FH FT source 1..400 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 400 BP; 49 A; 129 C; 153 G; 69 T; 0 other; gtcgtgtact ctttttccct tccaaggcac ccgttgcgag ggcggaggcg ccgatggccg 60 gggcgggagc gggggctgct ggccggtctc gcagcgtgcg cgacgctggc gtgcaacgtc 120 gcaggcgccg tgctcttcct cctcacccaa gacaacgagc gtctgcggcg cgggtgtgcg 180 gcgaggttgg gctctggcgg cctcgcgtca cacccggtgg aggagtgcgg agatgaagag 240 gcgctcgcgc cggacgccgt cctcaacctc gagctcggcg acccgacgat gtaccgggcg 300 ttctggcggg aggttgggga ctgcggcccc accgtgatcc gcgggtgcca ggggatgagc 360 tacttctccg acgacgacgc cgtgctctgc tggttcctcg 400 // ID DY239070; SV 1; linear; mRNA; EST; PLN; 476 BP. XX AC DY239070; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F15.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-476 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 5f8ac7981a3065ea234f9ec96a58bba6. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 15. XX FH Key Location/Qualifiers FH FT source 1..476 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 476 BP; 141 A; 114 C; 88 G; 133 T; 0 other; atctttcatt atataatcat gaatgaatga gaataacaca taattacatt tgtaccttcg 60 gcttgacaga aggtaagagt acaagcgtga cgcacgaacg agcacaaatc agcgtgaaca 120 gtacggggta ctgttcatct atttataagc acagggcgca gcctgtgtga aattacattt 180 atacccttta caatcgatta caatcataac acaaattatc atgggctgat ctgtcttttc 240 atctttaagt cgatgcaacc cttcgtctca aggcatgtca ttatgtcaaa gttcccatat 300 caccgcgagt caagaaacaa gcaacagaat ggcgtgcgag gtgatgcatc caaccccagt 360 tcctcagctg aatcatgata gcatcgatcc acggatatcc tgtctggcca tctctccatg 420 caacaaatag atcttcgttc tcgctccacg gaatctgctt gcatattttg ttccct 476 // ID DY239071; SV 1; linear; mRNA; EST; PLN; 476 BP. XX AC DY239071; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F15.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-476 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 8d34c152ce4e793376e5415cd345e279. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 15. XX FH Key Location/Qualifiers FH FT source 1..476 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 476 BP; 63 A; 186 C; 148 G; 79 T; 0 other; ctgtaacccc gcccgcgatg gaggccgcaa ccgccgccac ggcggcgatg atgtggttcc 60 ggaagggcct gcgcgtgcac aacaacccgg ctgctggacg cggcccgccg acacggggcc 120 ggcgctgcct ccgcgcgggt gtacccggtg ttcgtgctct atccgcgcta ccttcccccg 180 gatgccgtcg cggcgtcccc aggttccgcg cgcgccgggg tcgcccgcat ccgcttcctc 240 ctcgagagcc tcagcgacct cgacgctcgt ctccgccgcc tagggtcccg cctcctcctc 300 ctgcgcgccc gcgaagacgt cgccgactct gtctgcgcgg ccctaaagga ctggaacatt 360 ggcaagctgt gcttcgagtc cgataccgag ccgtacgcgc tggcccgcga taagaaggtc 420 acggattttg cattggcgtc cggcatcgag gtgctcacgc ccgtcagcca caccct 476 // ID DY239072; SV 1; linear; mRNA; EST; PLN; 406 BP. XX AC DY239072; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F16.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-406 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 58fb0739f372c4ef99506dc327390823. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 16. XX FH Key Location/Qualifiers FH FT source 1..406 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 406 BP; 69 A; 121 C; 69 G; 147 T; 0 other; gtgcactcat aacttgtcaa agccaaatac atattacaat acagaacgtc actattgcgt 60 gccttttaca accgtccaaa atgacgtcct tcatttggtt ttggttccaa atttcagcgt 120 atgcagctgt tctcaaaagc aagcacacgt gcggggaggc tttcctgtgg atcgcatctt 180 gcgcgccttc tctctgatct ttttcctatc gttgtatttt cgctcctccg ccatcgccct 240 tgctcctcca gagattttgc tgatccttgt catctccagg ttgtattctt ctagtgcctt 300 ttctctcttt ctgtccagct cggtctcttt ctgctctttt tggcgtctcg ccttcacctt 360 cttctcagtt tcccattccg ctatgggctc catcatcttc tcatac 406 // ID DY239073; SV 1; linear; mRNA; EST; PLN; 385 BP. XX AC DY239073; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F16.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-385 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; a61f81266ec8bfdae6374ee35aa9e90c. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 16. XX FH Key Location/Qualifiers FH FT source 1..385 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 385 BP; 85 A; 98 C; 106 G; 96 T; 0 other; ctcctatcgt ctgcctccaa gacaacagtt ttaatctgct tgcttgccct ctgctgcctc 60 tggttcacgt tcaccgcctc tattccgtgg ggtaaagcga gcatttttaa atacgagagg 120 ccctcgtttg tcactgaagc cgcggtttct tgtgccttgc aacctgccgt tggggcttct 180 tctctacaaa tactttgcgc gccatggatg gcgactcaaa aaatctgagg taaaagcggt 240 gcacgcaaca gggcctagag cctccgtctg ctgaaggggt tcggttttct ggagtaagaa 300 agggaggtag aggtggacgc cagggttcag cgataccacc tcaagaaagc gggcctattg 360 gaatagcgga tatgaacagc agcgg 385 // ID DY239074; SV 1; linear; mRNA; EST; PLN; 525 BP. XX AC DY239074; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F17.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-525 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 3ce5da10f736ff326c04d1da0963a109. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 17. XX FH Key Location/Qualifiers FH FT source 1..525 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 525 BP; 103 A; 157 C; 185 G; 80 T; 0 other; gacacgacga tcgatcaaat gagttcatgg acgcaatgcg ctactacacg ggaagggaat 60 tcacggtaga tctactatgg ttaggaatcc cagtttctcc accctgaaaa aacgagaacg 120 gcaaggtaag gcgcacaaaa ccagggtggt gtccaatgca aaagtgctcc ctccacatct 180 cgtggcgtgg cgaccggatg aaggcaggca gtgctgctac agggaccagt tcatcgcgct 240 ggtggtggaa tggttgaagg tgccgccgcc ggagccgcca tggtcgacgc gcggcaggtg 300 tggcggcccg ccgcttccct gcagggcgag ctcgagctcg ccggagaagt ggaccgccgc 360 ggagcctggg tggaccaggt gcagagcatc gtcgagacgc tgcgcgttcc tggcgccgtc 420 ggcagcgggg tcccgcgagc ccgtccaggc gctcgacgcc gcgtagctgc tcgccatgac 480 ggacggcgcc accggggtgc cgtcgaaggc gccggccgcg ggcat 525 // ID DY239075; SV 1; linear; mRNA; EST; PLN; 343 BP. XX AC DY239075; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F17.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-343 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 6069330dfa48dd97d6bd81ff94887df1. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 17. XX FH Key Location/Qualifiers FH FT source 1..343 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 343 BP; 68 A; 94 C; 134 G; 47 T; 0 other; cgctgccgcc taccctcgtt gaagggtgtt ctctcgttct cgttctcctg gtgcttcctc 60 ccactcccac tgaacagaga ggagggttag gggagggcgg ggtaggaagc taccgccgcc 120 acagtaacag cgttagctag gggggaagca cgaaagcggg aagcaatgga gacggggggc 180 ggcagcagca ggagagacga cgtgcgcggg ctcaagtttg gcaagaatat ctacttcgaa 240 caagacggcg ggagcgggag cggggcgggg gcggtgggcg gcaggaagga aaagggcgtg 300 cccaccggtg gcgcgaggcc ggcgtccgcc gcctccgcac ccc 343 // ID DY239076; SV 1; linear; mRNA; EST; PLN; 473 BP. XX AC DY239076; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F18.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-473 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 9fdce8cdd21a843a900a1ebf42bd3acc. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 18. XX FH Key Location/Qualifiers FH FT source 1..473 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 473 BP; 137 A; 114 C; 82 G; 140 T; 0 other; gcctaacaag accatcaatg agattaccag acagcagtta tacaagattt ctgtatctta 60 ttacactctt agcttacaag ggattgtttc ttacaaaatt gcacatttct aagttgctcc 120 tttgtttgcc gagtaggttc acaaatatcc agagtggcat actttggttc caacccacag 180 ttctagggct tgtttggttt gatgtctaac ttgccacact ttgcttaact tttttgccta 240 aggttaattc ttcaattcga acgactaacc ttaggcaaag tgtggcatat ttagccacga 300 accaaacagc cccctagtaa ggagctcaac cctaccctaa agttcatcag cacgagataa 360 gcctgtccaa aactgatgtc tggccttgaa gctcagggct gtcttatccc ccaaattctg 420 aatactctac atgccgacac actggacctt acataagata tggaaacaat aat 473 // ID DY239077; SV 1; linear; mRNA; EST; PLN; 503 BP. XX AC DY239077; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F19.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-503 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 1a505ecfe4563552b4b47d946d5399c2. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 19. XX FH Key Location/Qualifiers FH FT source 1..503 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 503 BP; 117 A; 170 C; 129 G; 87 T; 0 other; caaaaaacat gacaacaaat agtcaaatac agtccatgtt tttacaaaca atgaactcaa 60 ctcggttgct tctacacggt tccctaaaaa gtaaaaaccc caaatacacg ccttgacgcc 120 taaacacaaa acaagcacct cacataaaca gcggacaccg tcgctcacgg ttgaacggcc 180 tatccacaca tctgagcatc gccgcctatt cacgcattgc gttcagtagg cggggttgga 240 aaggatgact cccccggccc ggggagggtg cagcgcacag gcgcagtgca cggaggttag 300 gctgaggcac ccgctgatgt tgagcgagtg ccgctccggg cacgtgtgca gcgccgggaa 360 cgagtcccac accgcctgca ccgcgggcgg ggtcagcgcc gtgcactggc tgatgttcag 420 gctcgccagt ccgtcccgct cccggtcctt gccaccgccg ccgctcttcg ctgtcgcgtc 480 ccagccccgg ccccaactcc tca 503 // ID DY239078; SV 1; linear; mRNA; EST; PLN; 554 BP. XX AC DY239078; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F19.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-554 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; a36b0c0d243e304ced909041bb24e6f0. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 19. XX FH Key Location/Qualifiers FH FT source 1..554 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 554 BP; 126 A; 139 C; 158 G; 131 T; 0 other; ctgcgactaa aaaccatctt tggggccctt cgccgcgatt tgcttcgctt ctcggactag 60 gttaagttgc cttttcgatt ctcagaaaaa gacgggctct gcactcggat aattttaaaa 120 gggatagtgc acagcacccc actggggaat tgaacaattc gttcgatact cttatggttt 180 cgggaggtga ccagagtcga cagcctcggg atgctggcaa tgaaacgagc ttcacgaatt 240 ggaaagacct ccccgtggag ctgctcctgc gtatcatttc actagttgga gatgacggga 300 tagtcattgt ggcctccggt gtatgcacag gatggcgtga caccttgggg tggggggttg 360 caaatctttc actatcatgg tgccgggacc gcatgagtga cttggagata tcactggctc 420 acaaattcac gaagctgcaa gttctatctc tacgccagat cgggcctcag ctagaagaca 480 gcggcgtgga ggccgtggca aaccactgcc acgacctgcg cgagctggat ctcagcagac 540 gcttcgggct gagc 554 // ID DY239079; SV 1; linear; mRNA; EST; PLN; 473 BP. XX AC DY239079; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F20.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-473 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 4cc8c10fe6ccf06eaed4385c0ebe3dd3. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 20. XX FH Key Location/Qualifiers FH FT source 1..473 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 473 BP; 134 A; 124 C; 115 G; 100 T; 0 other; aaaaaaaagc gagtatagtt attctcgtcc aaccaaacca cacataacag gtttttccaa 60 caagataaat tcatgactca ggtaccagta attcaaacaa acatactgat ataattcaac 120 agaaatatgc gattctagca gggagcggca aaactaattg ggccttgggg tcccccactc 180 gactcggagg aaaagggtgt cataaccata cccgttgagc ttggtgatgg ccttctcggc 240 gtcctctctg ggaacaaagt tgaaaaaccc aaaccccctg ctcgacccag tcttctgatc 300 caccgcgacg taaacacggg tgacagggcc aaaggcacgg aagagctcaa ggaggtcagg 360 ctcgcgggtg tcctccgaga ggttggtcac acggacggaa ttctcgtcgt tcctgcgggg 420 catcgagtct ccagtcctat cagcacctcc tctcagggtt ggaggaacat ttg 473 // ID DY239080; SV 1; linear; mRNA; EST; PLN; 432 BP. XX AC DY239080; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F20.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-432 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; ed9af9d52f6c91add16e3380dc23bb87. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 20. XX FH Key Location/Qualifiers FH FT source 1..432 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 432 BP; 84 A; 160 C; 133 G; 55 T; 0 other; ctccgctcaa cgcaccgggc ggcacccaaa tcccaaaccc taccacaccc accatggcgg 60 cgcagaagaa gatccggtgg ggggagctgg aggaggacga tggcggggac ctcgatttcc 120 tcctcccgcc ccgcgtcgtc gtcggacccg aagaaaacgg cctcaaaagg gtcatcgagt 180 accgcttcga cgacgacggc aacagggccc gggtcacgac caccacccgc gtccgcaaac 240 tcgctcgggc gcgcctctcc cgcagcgcca tcgagcgccg ccagtggcct aagtttgggg 300 acgccctcag ggaggacgcc ggctccaggc tcaccgtggt ctcccccgag gagatcctcc 360 tcgagcgccc ccgcgcccca gggagcaaag ctgaagagtc agctgcatct ggtgatccgc 420 tggctatggc ga 432 // ID DY239081; SV 1; linear; mRNA; EST; PLN; 585 BP. XX AC DY239081; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F21.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-585 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 1abd9c0f3cadee5fc9ffac2179011fd4. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 21. XX FH Key Location/Qualifiers FH FT source 1..585 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 585 BP; 89 A; 186 C; 194 G; 116 T; 0 other; cgccgccgcc ttgaccgaca cggttccgcc catcgtgttc ttgcagtgcg caatggcgcc 60 ctcgatagcg ctctggagct cctccatgga cgatgccgtg gacaacgaca ccggaccgac 120 gccaatgccg ctggcaacgg ccggcacgtc ggggaagccc acgccgccag ccccgccaaa 180 ggtgagcaga cccgagtggc taggcgacga ctgcatcgat gacgggcagc tcgccgtggc 240 ccgcttctgg cgcgggaacc agaggtttcc ggagaaggag ctggagtgcg cgtacttgtt 300 gccgccaacc tctgctgccg cctccaccac tgccgcgacg gcgtcgccct ttttcttgcg 360 gatggagaag gtgaagggct tcttgaacct ctgctgcggt tggctgttgc cgttgacgac 420 gttgttctgg ttcttgttgg ggatctggga gagcctttcg tagagcggtt tcaccttctt 480 agcgtacttc ttgatcacct ccttggcgag cgacttggac ggctgcttct tcgcggccgg 540 agcgccggcg ccgccgcggt ggaagaccga ggagaagcgc gtggc 585 // ID DY239082; SV 1; linear; mRNA; EST; PLN; 374 BP. XX AC DY239082; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F22.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-374 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 46e0e14c95fa3d148b4ae5da3f4475c1. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 22. XX FH Key Location/Qualifiers FH FT source 1..374 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 374 BP; 67 A; 103 C; 98 G; 106 T; 0 other; cattcctcct ccaccttgct agctgctgca aggagataca tgcatacttt tcatggcgac 60 aatcggaggt tcacttccaa catgagcccc tgtgcgctgt cgtcagacaa agagtacccc 120 agtatggatc ttgcagcttc gtgcctgagg gggggccctt gcgactattc cgtggactct 180 gtcgtggggt tctcttggtg cactgcaaaa ctctgtgctt tgtcctgtgt gctaccgtgg 240 aaagtaaagg ggagcttggc cctcctttca cgggtctagc tgctctatgc ctattgccta 300 ccctattgac gctgttagtg tcctccatgt ggtttgagta gtgcccttgt gcaccagcac 360 cacagatctg gagt 374 // ID DY239083; SV 1; linear; mRNA; EST; PLN; 535 BP. XX AC DY239083; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F23.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-535 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 36d2877271d5f5485b08309e8b10137c. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 23. XX FH Key Location/Qualifiers FH FT source 1..535 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 535 BP; 171 A; 146 C; 94 G; 124 T; 0 other; gaaatgaaaa gcagggccgc aggaggaaag cttgctataa caggacagca aaaccgattt 60 tcctgcaaca acagcatcac attgcatgcc accacacctc taaatgacga cgtaaaatgt 120 ggagcaaatc tcttcattgc tccacattgc cgatgtgtcg ctacacaaaa ccaacgagca 180 ttacaaatat tcaacctgga gcacagaata ctatccaaac agaaacatct cgccatccac 240 cactctactg gcattctggc aacattatgg gatctcatcg ttggctaacc ctttccatca 300 ggtctctcat ttagcaataa taataatgag tacaaaaatg catttctgtc accaatcaca 360 cttacgccaa gcatcacaga tagcatcaag ccatccacgt ccaaaccaga tgccacaccg 420 ccaaacctac agcacttgag cagcagtgtt caacttctcg gcgatgctga tttgtgtcct 480 tgtgatgctt gacaggtata ccagcgcaag gttatcctga atcttgtcat tgaaa 535 // ID DY239084; SV 1; linear; mRNA; EST; PLN; 513 BP. XX AC DY239084; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F23.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-513 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; eda86c011e905ac5a83c427ae2d0dd10. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 23. XX FH Key Location/Qualifiers FH FT source 1..513 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 513 BP; 89 A; 180 C; 133 G; 111 T; 0 other; cgctacccct gccccttctc tcttcgtggc gcctccgcct ccgccgtcgt atcatcctcc 60 tccgccacct cggcgatggc gacatcccca gcgctcctct tctctttctc cctcctcggc 120 atcctctgcg ctttcgtaga cgcgggggga ggcaggggtg tttttcaacg tctgagacag 180 ctacgtgggg cgccgggacc agggggaccg cgtcatcggc accctcctcg gttccctcct 240 ctcagagggc accgtccacg tccgcaactc ctatgtcgtc ccccacagcg agtcagccga 300 ccaggtcgcc atcgacatcg actaccacca taacatgtac gcctcgcacc agaaggagaa 360 ccccaaggag gtcatcgtcg gatggttctc gagtggattt ggtgtttctg gggggagtac 420 tctcatccac gagttctatt caggggaagt acaaaacccc atccatctta cagtcgacag 480 tggcttcact aggggggagg cttccatcaa agc 513 // ID DY239085; SV 1; linear; mRNA; EST; PLN; 401 BP. XX AC DY239085; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257F24.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-401 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; fb34e605b1129cdceaf10189684a6099. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: F column: 24. XX FH Key Location/Qualifiers FH FT source 1..401 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 401 BP; 85 A; 109 C; 124 G; 83 T; 0 other; gaagaagaag ctagcagcta gaagacgagc gacgagtagc tagctagcta gctatctaaa 60 gagctcatca tatcgctgct cgctctcatc caccattata gagaagagca gatcgagctg 120 catctggcag aggccgagtt gttgctagct agctcctgct tgctaaattt gcatcgtatc 180 cgatccattc catgaaaaag tcgtcgatga tggcgcccat gacgatcatg gcgagagttg 240 ccgctgtgct cgtcctctcg tcgggtgccg tggcttccgc cgcgggagca gctgggctgg 300 acatgaattt ctacggcagc acgtgcccgc gcgtggaggc catcgtcaag gaggagatgg 360 tggcgatcct cagggcggcg ccgacgctgg ccggcccgct g 401 // ID DY239086; SV 1; linear; mRNA; EST; PLN; 407 BP. XX AC DY239086; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G02.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-407 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; ad8e602c73a862f4b7d6a81c2d1e07e8. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 02. XX FH Key Location/Qualifiers FH FT source 1..407 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 407 BP; 109 A; 117 C; 93 G; 88 T; 0 other; caataattcc ggcacggccc tgacccaaca acacaagggc gccacttatt cattcagatt 60 taggaactca aaaacagact cgaacatacc gcataaacag cataaatata attattatta 120 cgccgttccc ataaccagat caccccattt attcattcac atgcccttga gtctatcccg 180 ggatgagcac tatggaccct gatgtcttcc tgccctccag atctgcatgg gcctgggcag 240 cttccgacaa tggatacgtg tggttcaccc ggacgtgcag caccccgtta gcgacattgg 300 caaacacctc gccggctgac tccagcagct catcacgggt agcagtgtaa tgcatgaggc 360 taggacgagt gaggaacaag gacttggaag ccaggtcgct cgttggg 407 // ID DY239087; SV 1; linear; mRNA; EST; PLN; 444 BP. XX AC DY239087; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G02.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-444 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 5e468fce9f83dbc3818e7bd2ce17db48. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 02. XX FH Key Location/Qualifiers FH FT source 1..444 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 444 BP; 82 A; 143 C; 125 G; 94 T; 0 other; cgtttttctt ttttcctgga cagccactgc aactcctccc aaatcctccc acccggttta 60 gccggctccg ctgccaccgc cgcgactcca cctctgtcgc caccatggta aagtccaagg 120 ccatcagggt ccaagagctg gggggccccg aggtgctgcg gggggaggag gtggaggtcg 180 gagaccccgg agaaggagat atccgcatca agaccaccgc cgtcggcgtc aatttcatcg 240 atatctattt tcaaaagggg gtctacgccg cgcccaccat gcccttcacc ccaagaatga 300 aagccgttgg cgtcgtcacc gctgttgggc ctggcctcac tggcaggaag gtgggagatg 360 ttgttgcata tgccggcaac cccatgggct cctatgctca ggagcagatc cttccagcgg 420 gtgtcgctgt tcctgttcca cctt 444 // ID DY239088; SV 1; linear; mRNA; EST; PLN; 601 BP. XX AC DY239088; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G03.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-601 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 2012cc2a0d04a2174a854892194bca33. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 03. XX FH Key Location/Qualifiers FH FT source 1..601 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 601 BP; 77 A; 263 C; 186 G; 75 T; 0 other; cgagaaggcc gaagaggcgg cgcacccgga gaggcaaagg caagcgatcc ctctccctcc 60 ctccctcgta tccaggacaa cagcccgtgc gatccgatcc cgatgggggc ccggcgggac 120 gcgcgcaggg gctagtgcgc cgcgacgcgc actttccgta gcaagcgcgc ggacgtcccg 180 ctccccgccg acgcggacct ggacgtcgtc gggttcctcg cgtcccgccg ccacgcgggc 240 gtcgtcgcgc tcgtcgacgc cgccacgggc caccgggtca cgttccagga actctggcgc 300 gcgggggagg gggcggccac cgcgctcgcc gcgccgccgc tgtcgctccg caggggccag 360 gtcgcgctca tcctctcccc gaactccgtc cacttccccg ttgccgccct cgcagccgtg 420 tccctcggcg ccgtcctcac caccgccaac ccgctcaaca cgcccgcaga gatcgccaag 480 caggtcgccg acgcgcgccc cgtcgtcgcc ttcaccaccc gagacctcct ccccaagctc 540 gcgcgcgccg gcgccggcgc cggtatccgc gtcgtcctcc tcgagccaga ccgcctcccc 600 t 601 // ID DY239089; SV 1; linear; mRNA; EST; PLN; 537 BP. XX AC DY239089; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G04.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-537 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 3ddde9e437a7e66eb5c9a78067687131. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 04. XX FH Key Location/Qualifiers FH FT source 1..537 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 537 BP; 82 A; 248 C; 120 G; 87 T; 0 other; cttccacgaa tggccctcgt tctgtgaact cgtcgggttc catggcggcg ccgtcgccta 60 aacccccaac accacgcggg cgctgccacc acccgcccaa cgccacccgc cgcctccacg 120 tggccacccg ctgtttccca gacccaccac caccacctgc ctccatgccg cagatccaga 180 gtcaaaaccc tgtgctgtcc cttctctccg cggtgcccga gtgggccgac cccatccaag 240 agcgccgcgt ccgcgaccgc cgcccgctct aagaccacgc cacgtggggg caccaccgca 300 tctcccgccg ccacgtgcgc cacctcctca cctcggtgtc ctcccgcgcc atcctctccc 360 tggcgccccc ggtgtccgcc ttcaccgcct tcgccgccgc tatcgccaca taaaacacgc 420 tcctaccggc ctacgcgctc accgcatcct cgctccccta agagctcagt gcgcccgcgc 480 tcgcgctcct cctcgtcttc cgaacagagg cttcctacgc gcgctttcaa aaaggga 537 // ID DY239090; SV 1; linear; mRNA; EST; PLN; 454 BP. XX AC DY239090; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G05.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-454 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 1f5bbc3e6b0ae41268fd0be63455a941. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 05. XX FH Key Location/Qualifiers FH FT source 1..454 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 454 BP; 143 A; 103 C; 87 G; 121 T; 0 other; gactttgaat ccatcatata ttactccgca ttacacccca aaatctggga actttaaatt 60 ttgctataaa aggcttgatg gaaaatgatg ttttcgccta ggacagatac tggaagcaaa 120 aaaacgaaat tttacatcaa gttaaactat ggatggcttg cataaaccat tgtgctatcc 180 aatggtttat cagcttaacc gaagtggaga taacttcgtc ctcacttgaa gccacacggt 240 tctctagata tatgcccata attccgggtg atgaacagta cacaacagat tactaaatac 300 acctcggaaa agtggttcat gtctgcatac tctaccaaac ataactgagg gaagccgtcg 360 catcagattc tgtgatgaag gtcgacaacc aagtggtgtg catcatctag gagcttccag 420 acatctagct gcccggccat gctattacac tccc 454 // ID DY239091; SV 1; linear; mRNA; EST; PLN; 518 BP. XX AC DY239091; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G05.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-518 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 51ff94ee2cc97e5d556ffe28e42956a1. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 05. XX FH Key Location/Qualifiers FH FT source 1..518 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 518 BP; 95 A; 144 C; 161 G; 118 T; 0 other; cgcgcacgcg ccgtcgcgaa gagctccgcg tccgcatcgg ccaccgccca cccgcgccgg 60 ccgccggcgg ggtggatggg gaagctcgcc gccctgaagc gggaagcggt cggggtggac 120 taggtgctcg gaaatttcct attttagatt ttaaaagcag taggtggagg tgatgcgggg 180 cggagcgatg aaggctctgc ggagatccag cacctcctcg ccgccgtttt ccccgctttt 240 tctttcgtgg atccaccgcc ggtacctcct cgttacctcg tcggagacgt cctctttgtc 300 tgcatcggaa aattcggccg cggagggttc ggattcagcg tcagtggtgg cagcttcctc 360 gtcgccctcg ttggatcctt cgtctccgaa catggaccgg ggtgtaatta aatctccgtg 420 gtctcgaaga aaaaggaaac gagcgctcac ttgtcaacac tggatttgtc tattttcagc 480 gaacggaaag tttcgtgatg ggggaagaaa gtttctaa 518 // ID DY239092; SV 1; linear; mRNA; EST; PLN; 445 BP. XX AC DY239092; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G06.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-445 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 086b210fffd0afc1d62c22a216db9eb6. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 06. XX FH Key Location/Qualifiers FH FT source 1..445 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 445 BP; 131 A; 114 C; 87 G; 113 T; 0 other; gagttgcaac ttgcaacaat acccagattt ttataaaatt gtcttgtata gtactaccta 60 tgtacataaa ggcgtcagcc cacatacatt acacggaagt catatttgta gtgcagtacg 120 aagtgcaaaa tagcataatt gcacaatttt gagatagaga cgttcccatc aacatttgtt 180 gcgagaaccc tccaatccat tgccaacttt ctgaacccac cgtttcaact cctcaaacag 240 cataaaggcg aacaacctgg ttaatcgtgg cacggcagat agggcatccc cacttcttgg 300 attctatatc cttcaaacag gacatgcaac cagccatgtg gccgcacggg atgcaggcgc 360 cttccacagg ggcatccaga catataacgc aagtaccaga gggagttttg ccgctgtcac 420 ttgcatcagc ttcattttct aactg 445 // ID DY239093; SV 1; linear; mRNA; EST; PLN; 582 BP. XX AC DY239093; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G06.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-582 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; deef90a862b670abc8c01db5ed843fce. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 06. XX FH Key Location/Qualifiers FH FT source 1..582 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 582 BP; 113 A; 201 C; 162 G; 106 T; 0 other; cccgacccga accaccgcgc attaaaatct gccccctcct gccactgcca acgaccaatc 60 caatccctac ctgctcggga accacccgtc gggctcctcc cgcccccctc gccgcatctg 120 cgtgcggggg ggatctccgg gctcgctccg tcaccggagc cccaatcacc gccacctcat 180 ccaagccccc aaatcccccg ggcgcgcagc acaaccttgc cgcgatcgcg cgcggaaacc 240 gccgctgatt gggtcgtgaa agctcgattt tgggcgattt tgattcagaa aacccgccgt 300 ctttcgattt tggcgcgcgt tgcgagccgg gatggggttg cagcactcca aagaggaact 360 gctgtaccag caggtcaact acggcaacgt cgacgggatt cgcacgctcc gggggcaagg 420 cgctggtctc gagtggattg acaaggaagg aaacacgccc ctgatgcttg cctgtatgcg 480 cccggatctg ttcgacgtgc ccaaggtgct gatcgagttg agagctaatg tgaatgccta 540 caggcctggg tcacactgtg agacagcatt gccccatgct gc 582 // ID DY239094; SV 1; linear; mRNA; EST; PLN; 520 BP. XX AC DY239094; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G07.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-520 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; eba2efd2355b3e8f6afdcc6d52c94a83. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 07. XX FH Key Location/Qualifiers FH FT source 1..520 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 520 BP; 134 A; 138 C; 95 G; 153 T; 0 other; ctgttatcgt tcagtacact tgaaaataac ccagtgccat aatcataaca acgttatttc 60 ttccatgggc catttactac caaataaatg caacataacg cctaggtttg gccttgtacg 120 tccccaccaa gaccagaagg caaaataaac agactcgcta cagtggaggc atcacacgac 180 agatgtacaa agtttactca actccggcgt caaaatgtct caatcaaggt atcagcaggt 240 cccattctgg gagagattgg taagggctaa ttgaaactac gccggtggtt gcctcctcct 300 ttttggtcgg acgcggtcct cttcaaaatc cattattcct ccagattcag agagaaactc 360 gtcatgcccc cgtaagtcaa tgtctgcctt gattgatttt ttggcctctt ccactctgtc 420 ttcaaattct ttctggtgct tcttgttcac ctcactcatt gtgtcagccc acttgatttt 480 ctcctgaatt tgatgcaaca gtctatctga tgtccccaac 520 // ID DY239095; SV 1; linear; mRNA; EST; PLN; 549 BP. XX AC DY239095; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G07.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-549 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 52ba9ba16b98be34b65e49caf59a9b81. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 07. XX FH Key Location/Qualifiers FH FT source 1..549 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 549 BP; 123 A; 165 C; 145 G; 116 T; 0 other; cctagcgctc ccgacgcgcc gcggagcgat ggcgatggac gcggagcggc ggcaggagga 60 gctgatcgcg cagttctccg ctcaggcggc ggcgctttcg cccgcgcccc agctcgccgc 120 gctggtgctt gaggccacct cccacccggc cctcttcgcc ttctcagagc tcctcaccct 180 cccggccctc tccaagctca ggggcacgca gtacgcgtcg tcactggact tgctccggct 240 cttcccctac ggcaccctga atgactacaa gagtaattct ggattccttc cagcactgtt 300 gcctgaccag gtccgaaagc taaagcagct cagcgtgcta actctggcag agtcaactaa 360 gatactacca tatgatcagc tcatgcaaga gttagacgtt tcaaatgtaa gagaactcga 420 agatttccta atcaatgagt gcatgtattc gggtatcgtt agaggcaaag tgaatcagct 480 gagaaggtgc tttgaggtac aatttgcagc tggaagagat cttacacctg atcagctaaa 540 gaacatgat 549 // ID DY239096; SV 1; linear; mRNA; EST; PLN; 533 BP. XX AC DY239096; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G08.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-533 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 440cd7662adadaa945526f6f5a38148a. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 08. XX FH Key Location/Qualifiers FH FT source 1..533 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 533 BP; 146 A; 150 C; 103 G; 134 T; 0 other; ggtaataaag tcatcgcata tcttttgcgg ttcacagtta catatcattt accagccccc 60 caggcggctc cagcgatgta cacccacgta ttgtatgatt gtatctgaaa aaacatcatc 120 tgagtatctg acaactccca aatccaccaa tgctccgttt cttatgagag aaccaacgtt 180 ccggtcatca agtgcgaagg taggaccaca caatatacat catcacacgc agcgacacaa 240 caggggctgc gactgcgaac cttatagcca gacagccgtg ctgctacttt gtaaggtagt 300 atatgagcaa gaagacagca atgaaggatg ccaccatggt cgccatcctc cggctcgatt 360 tcgtctcgaa aaccatcttg aacttgtcca ccgtccctga aagaaatcct ctcgaagcat 420 ccatgtcatt acccattctg tccagcatcc ggttatggtt ctccacttct tcatgtatat 480 cgccagtcag tcttttgaga acgctgaccc tatcatgcaa actgtccaga gct 533 // ID DY239097; SV 1; linear; mRNA; EST; PLN; 485 BP. XX AC DY239097; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G08.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-485 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 0e43c3e2ac918ae01220992051142e1c. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 08. XX FH Key Location/Qualifiers FH FT source 1..485 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 485 BP; 137 A; 101 C; 134 G; 113 T; 0 other; tcaagaagga cggaggaggc gcatcaagtt ttcataaaat ctcgccgccc acccacaccg 60 ggagggaggc tccgtcggct tctccgcacc cgagagaact cttcggccag agaaggtcgc 120 gtcagatgaa ctcgaaaagg gacttccgta cccatagagc tgttttattt gatggtattg 180 aaaaaggggg ggagagagct ccagcttatt cttctcgtga aattcatgag catgaaaatg 240 atcaagctct ggacagtttg catgataggg tcagcgttct caaaagactg agtggagata 300 tacatgaaaa agtggagaac cataaccgga tgctggacaa aatgggaaat gacatggatg 360 cttcgagagg atttctttca gggacggtgg acaagttcaa gatggttttc gagacaaaat 420 cgagccggag gatggagacc atggtggcat ccttcattgc tgtcttcttg ctcatatact 480 acctt 485 // ID DY239098; SV 1; linear; mRNA; EST; PLN; 304 BP. XX AC DY239098; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G09.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-304 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 78e95a39391baffdfd5ae98e4b0a3bf7. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 09. XX FH Key Location/Qualifiers FH FT source 1..304 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 304 BP; 74 A; 70 C; 69 G; 91 T; 0 other; ggcaccgaag gtttcctcta gattctggga cctgctcctc tgcttgtttc aaacgcctca 60 caaagaggtt ggtggtcttg ttagctgcag tagtgacggc ctgtggcatc ggcgttctga 120 gtggttcaaa accctgaaaa attaaactaa gctatttgct cctcgttatt tccagtcgtt 180 ctgatgtcaa acggggccct cgtcgcttcc cctagcaaac gcaggatagc tgtttctgca 240 tttgtcatta ggaaaaaaaa aaaattaagc accgtattat tgtcaataat tgttggttga 300 cacc 304 // ID DY239099; SV 1; linear; mRNA; EST; PLN; 516 BP. XX AC DY239099; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G10.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-516 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 8a5dba5ebbdaef161b89d9ec59fb5bd5. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 10. XX FH Key Location/Qualifiers FH FT source 1..516 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 516 BP; 113 A; 160 C; 142 G; 101 T; 0 other; ctcgccccct ccctaccggc accgccaaga tgggctgctc ccacatcgcc gccgcggggg 60 tctcctcccc cgccgcagcg gcggctcgtt ccccggcgca ttctcccgtt tccgccttcg 120 cgcgcctccg gtagacgcct cgtttcgaga gcgctggttt gtcggtaaag gaaaacggag 180 cggcgttccc gttggtcgcc gccgcggggc cggccgcggc ggcaccgggg gcagacctgg 240 acggccgccc ggccacggaa aaacagccca tcatcgtcat cgaaaaataa gagagcttca 300 catacaacct ctgccagtat atgggggagc ttggattgaa cttcaaaata taccgcagtg 360 atgaactgac cataaaagat gtgagaagga agaacccaag gggaatactt atttctccag 420 aacctggtga accacaagat tcgggaatat cattgcagag tgttcttgaa ttaggcccaa 480 ccatcccaat ttttggagtt tgcatggctc tgcaat 516 // ID DY239100; SV 1; linear; mRNA; EST; PLN; 621 BP. XX AC DY239100; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G11.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-621 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 817e34a83cf961cdf12fe8385f006c7b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 11. XX FH Key Location/Qualifiers FH FT source 1..621 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 621 BP; 87 A; 234 C; 201 G; 99 T; 0 other; acgttctgct tgtccaggta cgtggtcttg agcgcgttcc acccctgctt gtggatggcc 60 atggggtccc tcagcaccgg gtgatcgctc gggtactgct cgctcagcgt cgtctcgtcc 120 agctgcacca cgtactccag gtagtggacc tccatccccg ccgacggctc cgcgaacgtg 180 ttccgcgcca gccactccag ccgcccgtac ggcaccacct gcaccaccac ggcgccggca 240 ggcaggaaca ccatgttggt gagcccggcg ccgtggacgc ccaccatcac gtcgcacgag 300 ttcaccagcc gcgcgaactt ggacgtgtcg gtgctggtgt ctggctcccc gatccgcacg 360 tcgaacccca ggctggccgc catgtccgcc atggcgcgct cgttcatgaa ggcgcgcccg 420 cgggacgggc ggcgcgagat gatgagcagc cgggggcggc gccggatgtc ccaccggtcg 480 ccgctgggcg tggccgtggc acgctccagc ccgaacgcgt cgcgcagcat cttgcggaag 540 tcggccgtgg agtaccccgg cggcgatttg gacgcgtcca cgcccagctc ccggtggaac 600 gtcgccccca ccaccacgct g 621 // ID DY239101; SV 1; linear; mRNA; EST; PLN; 548 BP. XX AC DY239101; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G11.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-548 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 99c420127ea89109509cbe2211e1cd2a. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 11. XX FH Key Location/Qualifiers FH FT source 1..548 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 548 BP; 108 A; 178 C; 179 G; 83 T; 0 other; gagagggtga gatgaagact cggagctcaa caagagggga gccgaggcgt ctcgaaaatg 60 cggttcttct ggtgctcatg ctctgctccg tcgtcgcgct ctccctcatc cgggggagat 120 tccccccatt tgtcactacc gcgggagatg ccatcaaatc cgaggacgac gctgcggcgg 180 ctgcgctcag tgtcagcagg gcggccgtca acgttgacac cggagacagc cccgacgagg 240 cccccgccaa atctgctgct gaggaaaaag aaaaggagga agtccagccg aaccccgctg 300 acagttccgg ctccggttcc ggtggcagcg cacccaagcc ggtgtgctac gagacgagcc 360 gccgctccga cacctgcgag gcatctggcg acgtgcgcgt cgtggggagc acccagacgg 420 tgtacgtgga ctctctggac cgggagtgga agaccaagcc gtactgccgg aagcacgaca 480 acttcgcgct cgcccacgtc aaggagtggt ccctgaagcc gctcccctcc ggcgcggcgc 540 cgcagtgc 548 // ID DY239102; SV 1; linear; mRNA; EST; PLN; 505 BP. XX AC DY239102; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G12.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-505 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 3e115203fee06f2fc41010d5f1081043. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 12. XX FH Key Location/Qualifiers FH FT source 1..505 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 505 BP; 128 A; 159 C; 123 G; 95 T; 0 other; gagtatcaaa tagactgaag cagcagcaac tgtacaaatg acctgtcatg gccgagatgg 60 aagaaaatgc cctacatcac ccaaccgagg agggggggaa gaacatcaag ttttttttac 120 ctcaaataaa acaacacccc cagagaagaa ggggggaaga aaggaccagt tgagaaaaac 180 aaggcagaag atgatgtaca gacgcagacc cactgatcaa ctcaaaacac tactctagca 240 tgcatctcct agcactggta cagcgagaag cccctgttct tccacagcga gctcagcgtc 300 ctgcccagct tcctggactt ggacttgcac agcggcttcg attcccggtg gcacacgata 360 gctccaccac caccgttccc gccctccgcc agctggctct gcagcagctg ctgctgctgc 420 tgctgtggcg gcggcgtgtc ccgctgcctc tgctccctga acagcttcag gagcttctgg 480 gccttctccc tgcccctccc cgtcc 505 // ID DY239103; SV 1; linear; mRNA; EST; PLN; 549 BP. XX AC DY239103; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G12.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-549 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 1b6ba9ae583d16a9e0c10a94e2ce407b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 12. XX FH Key Location/Qualifiers FH FT source 1..549 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 549 BP; 96 A; 164 C; 152 G; 137 T; 0 other; cgctcgtcga cggtgacccc cgcggagccg cgtgaatacc tcgccgggaa gatctgcctg 60 ccgtctctac gggcaggtac cagagttgga gcgtggttac tggcgggctg tggctgcttt 120 cggcctcccg ctcctcctac acgcgcgcgc ccggcctctt tcagggttgg ctctgaagac 180 aaagcccaag ctccctgcgc tgctgcgcaa gcggccgacc ttcctgccgg cggtactccc 240 tgtgctggat cctctgtcta gtgttgcagc tgccagcgct tctcatcctc actctaccgg 300 agtggagatc cgtccgggcg tgggttgatt ccttcttttc gggtcagatg ttggagctta 360 ggtagtaccc aacgatggaa gcaatggagg ccgaagaagg tccttttctg gctaatgatg 420 ccaagttgca cgcaggaatt tgtagagcat ttcatccttc tgtatctaag ctttccgcca 480 ttttcccctt tatcgaagca tcaagaccca gaagcaagcc tggtatacaa gcattgtgtt 540 ctctacatg 549 // ID DY239104; SV 1; linear; mRNA; EST; PLN; 448 BP. XX AC DY239104; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G13.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-448 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 07b3d2beac237cb520234a0e0fd56fae. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 13. XX FH Key Location/Qualifiers FH FT source 1..448 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 448 BP; 80 A; 126 C; 142 G; 100 T; 0 other; caaaacgacg aggccctgat attatatgca tgcatgtgca gttgtgcacc atatagtccg 60 atccatagcg ctagtgcact cttgcttgtc ggtgtactaa cgacccggcc gggcacatgc 120 acatgcattc acgtccgttt ctaactacac acgtcgtcgt cgtggattaa tcatcattag 180 ccacccggca gtcgcagcgg acctctccct tggccgggtc ggtgagcggc tggatgttgc 240 ccatgttgat catggactgc gcgaagctgc tgaaaaaggc ggcctggctg gtggcgaact 300 ggtcgacgac gggcgcggtg acgccctgcg cctgcggcgc cgacttgagc tcctggtccg 360 agccgaggaa gccccggttc acctccaggt tggtgtagta gtggttgtcg aacaggttcg 420 gcgtcgtggg gtccaggtcg ttgagggc 448 // ID DY239105; SV 1; linear; mRNA; EST; PLN; 436 BP. XX AC DY239105; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G13.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-436 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 0859088ce55c67682c25ab580ad39bd3. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 13. XX FH Key Location/Qualifiers FH FT source 1..436 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 436 BP; 75 A; 145 C; 137 G; 79 T; 0 other; tgatattttt tccgacggac acacaagctt atatacatac ccgccgacat agggatgatg 60 gcttcctcta gtcctcagcg tgcagccggc gccgccgtcg ccggcgtgct gctggggggg 120 gctgccgccc tgtgctcgtg cggcgccgtg gcgcatctga ccgcggacta ctacgactgc 180 acatgccctg acgcctacaa catcgtgaag caggtcctga tcgaggcgca caagtccgac 240 gtgcgcatct acgccagcct cacccgcctc catttccaag agtgcttcgt ccagggctgc 300 gatggctcgg tgctcctgga cgccgttccg ggggtggcca actccactga gaagctggcg 360 ccggccaaca acaactcggc gcgagggttc ccggtggtgg acaaagtgaa ggcggcgctg 420 gaggacgcct gccccg 436 // ID DY239106; SV 1; linear; mRNA; EST; PLN; 381 BP. XX AC DY239106; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G14.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-381 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 55f34556aa4815f14790b249a31e781d. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 14. XX FH Key Location/Qualifiers FH FT source 1..381 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 381 BP; 88 A; 132 C; 110 G; 51 T; 0 other; cacgtactaa taactcgtag actccagcac ctcaaaaact catcgataca agtacacagt 60 gcacgcaggc acacgagaca ccaaactctc gacgtcgtcc aaaaaaaaca aacaaatccg 120 gcggacgaat caccgaagcc cgttccggtt ccggcgctct ccgtccgtcc ccccgcccgc 180 ccgctcaaaa aatcgcggaa agcgggttga gcgggaagag atcgcggagg ccctgaaagt 240 agtcgttctc ctgcaccggc gacgcggccc agctgggcgc gtcgtcggac gaggccggct 300 cggcggcggc ggcggcgttc cacgacgcct ccggcgacga cggttcggcg tagaggaacc 360 ccgcgtcggg ctcgaagtcc c 381 // ID DY239107; SV 1; linear; mRNA; EST; PLN; 434 BP. XX AC DY239107; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G15.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-434 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; a315643eecd9865d04994a38ef7abf08. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 15. XX FH Key Location/Qualifiers FH FT source 1..434 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 434 BP; 124 A; 132 C; 72 G; 106 T; 0 other; gatggcatac aatcttacta ataatatagt agctactaca gttctgtacc atgcaatcat 60 ccttgcattg tactgctacc tcaagcacac tgcatgcatc atttgcagct ggcccttcct 120 cgagttgcag tcataatttt tttacatgaa acacaaaaac cattcccata aacgaaaata 180 aacatgcaca tcatattctg gaacgaaaaa caaaacaggt agctgtttca gcagcaaaaa 240 ttgctcaaaa gcctggcccc cctccccaag tcccaacctt gaaggccacc ccgccaatca 300 ttcattccct gttctccgac gctgggcaca tgcagaacaa aaagatgctt ccgatcacga 360 tctccgactc cgtcccctac ctgttgccgt tgggatcgat ctcaaacgaa gtgccgtcct 420 ctgctgcccc gtgc 434 // ID DY239108; SV 1; linear; mRNA; EST; PLN; 523 BP. XX AC DY239108; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G15.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-523 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 6ebe4568804480ea6d48fe9dbf3f7494. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 15. XX FH Key Location/Qualifiers FH FT source 1..523 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 523 BP; 83 A; 170 C; 193 G; 77 T; 0 other; gcttgcttgt tttcctgccg cggccgctgc ttggccagga ccagctgctt gctgccgtcg 60 tctcgtctgc ggctactccg gcctctcatg ctggccacgc caccgacgct gcgatacaaa 120 gcgccgcggg gggcacgtca gaaatggcat cgtcgctggg agtggggggt cagcagcagg 180 ggcacgggca cgggcacgga gaggccaggg cgcggctcag ggggacgcgc cagctgcacg 240 accgcttcgt gctcgccggg gcccatctcg gcggcgcaga caaggcgaca cccaagtccg 300 tgctgagggc gatggccgtg ccggggctca cgctgtacca cctcaagagg cacctgcaga 360 agtaccgggt ggtggcggag agccgaggcg tcgcttcccc gctgggcgac agcggagatg 420 gcaccgaaga gcgttcgtcc tcgtcctcgg agaaccagcc ggcggaagag tgcgacgacg 480 gtaccgtcgc cgagccgcat ggcgattctt ccaagagcgt ggc 523 // ID DY239109; SV 1; linear; mRNA; EST; PLN; 493 BP. XX AC DY239109; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G16.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-493 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; ced8fb5ada7db96303021a0da46409c1. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 16. XX FH Key Location/Qualifiers FH FT source 1..493 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 493 BP; 131 A; 122 C; 102 G; 138 T; 0 other; gaacaatgtt tatcttgagt gtactctcaa gtgctgtgag atactattat tcaatggacc 60 acggtaaaac cgtacgacag acccattagg aacacatcca tttcctaagc aaaaccgtac 120 ggtatactca ccaggaacac atctgtaatg aagatttttg gtaaacgtta tttgtgtata 180 catggggcga atgcacatta ggcaaaagaa acataaagta tcaaaactgc tggtccagat 240 atgcatcttt ctctgacttg ttcccaccta ggtaccctgc caaactctca gaaacgtccg 300 tgacccagga gacgctgcga gtcagccaag ctttaggagt cgatgacatt tctggggtct 360 ctgccgcaat gcgcctttgt tcttcctcga gcaatatgtc cagccacctc tttgacatgt 420 actcttttac tgcatccacg cccttgttca caatgtcctc tggtggcatg actagagggt 480 tctgctctag gtt 493 // ID DY239110; SV 1; linear; mRNA; EST; PLN; 628 BP. XX AC DY239110; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G16.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-628 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; c811eedc68ca2e596cab8bc70fd597af. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 16. XX FH Key Location/Qualifiers FH FT source 1..628 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 628 BP; 94 A; 248 C; 197 G; 89 T; 0 other; cttccgcgtc tcttcgaaga catctagaaa aagcatcttc cgctactccg ccctccgcct 60 cccccggctg cttcgcaatc cgatccggac gacgggcggc catggatcct gcgccgcaaa 120 cgcacccgat cctctcttac gtgctctccc gcatccccaa cctctccatg accaagaccc 180 ccaccgccga gttcgacatc gagcagccgc gtgtgcacac cccgtccccg cggacgccgt 240 cctccgcggg agagttcgag ctcgtcgagc gcatgccggg gctgcgccac ccgtccgtgc 300 tccgcgccat gacccgcgcc gtcgcagacg tctccgccgc gcgctccgcg ctgcaggtgc 360 tcggcccgcg ccccgaccac gagctcgtcg actcctcccg cgccatagtg gccgccgccg 420 aggccgggga ttcgcggatc cccgcgggcg acgtcgaggc gtgccgcgcg gtggtgcgcc 480 tcgatgagac ccacgacgcc tacgaggccc tgctgcacga ggccgagtcc aggctcgaga 540 gggtgtaccg ctccgcgatg gaggggacgg acctggaaga cgaggcggcg gagagcgtca 600 aggatcaagg gccggtcgcg gggcctga 628 // ID DY239111; SV 1; linear; mRNA; EST; PLN; 627 BP. XX AC DY239111; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G17.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-627 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 02ddab4727944c2524de491ddfac929f. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 17. XX FH Key Location/Qualifiers FH FT source 1..627 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 627 BP; 136 A; 201 C; 167 G; 123 T; 0 other; attcaatcca acagcaacta ggtcacttat tatccatggt aacacatcgt agcagcacaa 60 gagagagcgc cagtcttgca ggagagagat ccattctcct gctaaactcc acaaacagat 120 agcctaaccc agacagaatt tctgaagcct aaaagacccc ccgataccga cgaaaccact 180 gcattccaat cagttgattg cattagccag gcacaggcgc tcagagcttg cccttgagcc 240 tcttcttcag cacatccatg acggcgccaa agtcggcctt cacgctcaca ggcgggttgg 300 agtacttgca agccatgtcc tcgatcttgc tgtcatggta gtcgatcttc gactgcgaga 360 acttgaggcc gtgagccgtg ctgacgacca cggtgcggtc gttcgtcccg atgacgcgct 420 gctccctgag cttgaacagg gcggcgagcg cgaccccggt gtgcgggcag gcgaacatcc 480 cggtgcggtc ggcgagcgac atcgcgttca tgagctcttc ctccgtcgcc tcctcgacaa 540 tgccgtccgt cgccttgagc gccaccacgg ccccggtcga cggacaccgg gtcgccgatc 600 tggatcgctg acgcgaacgt cggcctg 627 // ID DY239112; SV 1; linear; mRNA; EST; PLN; 598 BP. XX AC DY239112; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G17.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-598 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; c88c602ac22415b1bb2ffe3103d3d356. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 17. XX FH Key Location/Qualifiers FH FT source 1..598 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 598 BP; 92 A; 243 C; 174 G; 89 T; 0 other; ctcaccattc tcctccccta atcccactcc gccgccgcca ggttgcccat ggcggcaaca 60 acccacgccg cctccatgtc gttcctcctc tctcaccccc agtcgcgctc tgccacccca 120 agccgccacc tcccgctccg tcgggcagcc cgccgcgtcc agtgcgccac cgacgccgcc 180 gccttttccc cgggggtcac caccaaacac cggcgcgcgg gggaagaaaa catccgcgag 240 gaggcggcgc ggcacccggc cccaaaacag ggcctgtcgg cgtggtaaga gcccttcccg 300 ccggccccga acggagaccc caaagagcgc tactccctgg aagagatcgt gtaccggtcc 360 ggctcggggg gcctcctcga cgtgcggcac gacatggagg cgctgtcccg cttctcgggc 420 gcctactggc gcgacctctt cgactcccgc atcgggcgca ccacctggcc gtacgggtcc 480 ggcgtgtggt ccaagaagga gttcgtgctc cccgagatcg agcccgacca catcgtctct 540 ctgttcgagg gcaactcgaa cctgttctgg gccgagcggg tggggcgcga ccacctcg 598 // ID DY239113; SV 1; linear; mRNA; EST; PLN; 501 BP. XX AC DY239113; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G18.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-501 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; cd0fba8abafa1f59658b74e74c0f7a55. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 18. XX FH Key Location/Qualifiers FH FT source 1..501 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 501 BP; 140 A; 124 C; 112 G; 125 T; 0 other; agaaaccaaa acgaatttta atttatgaca gaaggagtac atgacattgg tttaatggca 60 agggcaacat ctaaatgcct tatattttta tagcgaaacc aaaaatcaaa atgtggcgcc 120 ggtgcactgg caagggcaac aggaaggggc ttcgctacaa tgctgatgtc caatggttat 180 tggcccgcct attgatgatc gagcacggtg tatgatgctg atgcatacgc ccttaacttt 240 ccagaaatcg ccacatagct gcagttctag cagtacagaa tactgatagg gcaacccgcg 300 tggtcgcttg gtgttgagct tccacaaaac atgagcttct tttctgcata gtcatcttag 360 cctatcctct tgaatttcag tccggacaat tgatacaacg acggctatgt catccagttt 420 gcccccactg tagccaagga acccagccga gagagcagca tctgagaatg ggcttctccc 480 agccccagac ctccctactt c 501 // ID DY239114; SV 1; linear; mRNA; EST; PLN; 471 BP. XX AC DY239114; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G18.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-471 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 180710c9efd6bedca425a937efd372dd. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 18. XX FH Key Location/Qualifiers FH FT source 1..471 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 471 BP; 87 A; 172 C; 134 G; 78 T; 0 other; tctttctctc tctctctctc acaggaggta agccatggaa aagttgcctt tggcagcggg 60 gctgatggac cttcgcaact gcaagctctc tccgaagcca gcgccgccct tgccactccc 120 cgcgctccgc cgcatgcaca caagcgccac ggatacaacg gccccgtccc cgcgccgtgc 180 ggtccccaaa ctccactcca ctaccgagct gggggagggg agcatcgtct tccgtttcgg 240 ccatcctaag tccaaggcgc tggagccgga gccggcgagc gtaaagacca ccccttactc 300 cagagttgcc cctgcctcgg gcgctgtgcc cggcagccag atggagccgg agattgactc 360 tgaacatgga ttacacggcg tgcgaatagg caccacggct ggggttgaga agccgagccc 420 gaatgccgca cccgaggcct ggccgacacc tccacctcct gacgccggcg c 471 // ID DY239115; SV 1; linear; mRNA; EST; PLN; 573 BP. XX AC DY239115; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G19.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-573 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; d6c3bd6bca27f7eaaf048005157ba699. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 19. XX FH Key Location/Qualifiers FH FT source 1..573 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 573 BP; 160 A; 151 C; 113 G; 149 T; 0 other; gatttgcaca cttttattgc tttctaaaag aaaaatagta caggtacaac aataatgggt 60 gtaatcagca catttttcat gcttcccaaa atttgcacta acaaagtaac aagtagtgaa 120 tagagataca aacataccaa aaactatgag tggaagttgt aaactataag ctgtgggcag 180 aagtcttaca caaggtcttg ctctaacttt tctgctattg tagatcatgg tcaaaagtgg 240 atcttcaaac aatcaaaaaa ttactcggtt cactgtggta tgcaagctag ttgggcatca 300 gtcctaatga ttcatatggg actcaaacca ttggaagact tcagtattct tcaggctggt 360 gaactcatgg tacgcatagg caacattgag ctcctcggtg acatgcctct ccagaccgaa 420 ccgctcaggc agcaccttgg cctcctcagc cgcctccttc cacggcgttc tcctcccgtc 480 cttcttcagc atccccttct catccacgaa tcccttccgc cgaagcatgt ccaccagcgc 540 gttggcgaag gcccaggtca gccccggcac cct 573 // ID DY239116; SV 1; linear; mRNA; EST; PLN; 629 BP. XX AC DY239116; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G19.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-629 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 27f8ef65bd0db7a2bc80e52ab4eea56d. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 19. XX FH Key Location/Qualifiers FH FT source 1..629 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 629 BP; 79 A; 252 C; 192 G; 106 T; 0 other; ctgatgccca aatctctctg ggattgggat ctccagttgc ccgatcccct tccatgacac 60 gcgaaatgca cgccatcctc gcgtgggatt ccgcagtccc tccggcccgc ggtgctgatc 120 gcctcgacgt gcgcgctgct cctcctcgcc accgcgctac tccttccgcg cgctcctccg 180 ggggcgccgg ggggggcgct gaccactgtc accgacgcgg acgccgtccg cttggacgct 240 agcgtcaccc gaaggaacgg caacgaggtg ctgtggcagc taccgccccc gtcgtcccca 300 ctgcgagccg cgttgttcgc ggcgcccggg tgcaccatcc gcgccaccga tttcttcgac 360 gcctccccgg ggtgcccgcg ctgcacgggg cttcccgagg agcgccgttt cacccgcgcc 420 gcgctcagcc gcgggtacgc cgtcctcgcc gtctccagcc gcgccaagtg ctggtccctc 480 caccacgccg gcggcgggga ggacggcaga gagctcgccg ccgtccagtc catcatcaag 540 tggtggacca cagagaaata ccctcagctc gcgggcctcc cactcttcgg tatcggcgcg 600 tcctcggggg ggcacttcct ctccgcgct 629 // ID DY239117; SV 1; linear; mRNA; EST; PLN; 362 BP. XX AC DY239117; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G20.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-362 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 0fa104b6635603b6839420f741fb19be. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 20. XX FH Key Location/Qualifiers FH FT source 1..362 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 362 BP; 92 A; 94 C; 93 G; 83 T; 0 other; aacacaatgc aggattgaat tggtccaaaa tctgtatcgg aaagcagaat acacaacaca 60 ggactgacag acttgtgttt gtgaacacag caccacgccc agaatacaca cggatcatgg 120 atgaagtttc gtggaggagg cctgaactcc gtcggatgaa caccgaattt tcacctgtat 180 gactcgacgt tgcgtttggg cttgctttgg accaccgcct cgatcctgtc gacgacctcc 240 ggcgtcagca gtgggatgac atctagggcc ttcatgttct ccacaatctg gctttctttg 300 gtggctccag tgatcacaga cgacacattg gggttcgacg cgcaccaagc aatcgagagc 360 tg 362 // ID DY239118; SV 1; linear; mRNA; EST; PLN; 362 BP. XX AC DY239118; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G20.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-362 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; bdaec59cda55bc05593de0efa46b6c52. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 20. XX FH Key Location/Qualifiers FH FT source 1..362 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 362 BP; 79 A; 93 C; 101 G; 89 T; 0 other; cagttctcga ttgcttgggg cgcgtcgaac cccaaggtgt cggctgtgat cgctggagcc 60 accaaagaaa gccagattgt ggagaacatg agggccctag atgtcatccc actgctgacg 120 ccggaggtcg tcgacgggat cgaggcgggg gtccaaagca agcccaaacg caacgtcgag 180 tcatacaggt gaaaattcgg tgttcatccg acggagttca ggcctcctcc acgaaacttc 240 atccatgatc cgtgtgtatt ctgggcgtgg tgctgtgttc acaaacacaa gtctgtcagt 300 cctgtgttgt gtattctgct ttccgataca gattttggac caattcactc ctgcattgtg 360 tt 362 // ID DY239119; SV 1; linear; mRNA; EST; PLN; 599 BP. XX AC DY239119; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G21.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-599 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 8da82766b6ff1da3c1514fa19c2620eb. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 21. XX FH Key Location/Qualifiers FH FT source 1..599 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 599 BP; 85 A; 220 C; 193 G; 101 T; 0 other; ggcgagcgcg agcgtggcaa agtagatggc gaggtacgcc tgcgccagca gcagcgcgcg 60 gtggaggagc ggcaggtacg ggcccatccg gcgcaccagc gcggtgagca tgaagagcgg 120 cagcagcagc accgccaccg acgccgcgga ggcggccacc gccgcccacc ggccgcccag 180 gatcgggcgc acgccgtcgg cgatcccggc gttcgccgcg gacaggtacg ccttggagtg 240 gtgcgagagg cgcgccaggt ccggcatcag cgtctcctgc agccgactgg gtaggtcccg 300 gaattcggag atgagatcgt ccgtgccctc ccccgcctcc ttggccaaca ccacctcctc 360 ctcatcctcc atctccaccg tctccgtcgc cgctgacttc tccttgctca cgacggtcgc 420 attcgccttc acctcggcgt cgacgaccac cggagccttc ttgccggatt tggccgtctt 480 ggcaccggca tccacggtcg aatttgccgg cggcggtttc tttgccttcc caaccgcggt 540 ggcagcctca gatttggccg tcttgggcgg ctttcaccgc cttggcgccc gtcttggaa 599 // ID DY239120; SV 1; linear; mRNA; EST; PLN; 537 BP. XX AC DY239120; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G21.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-537 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 433a2bcfce6b259ede67c60ab0eb93de. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 21. XX FH Key Location/Qualifiers FH FT source 1..537 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 537 BP; 95 A; 209 C; 158 G; 75 T; 0 other; cactcactca ctcgcggacg ctgccacaaa cttccgcccc tgatctccgt atataacacg 60 ccggcggccc gtccgtccgg gctcctgact ccggatccac cgccgccgcg ccgcactctc 120 cctctcactc caccgcaaaa gtactccccg cctgcctctg ccagcctgtg cctgcacaaa 180 agcaaaaatg gcgccttggc cgcggcggcc cgtgctgacg ctggtgctgc tcctgctgct 240 cgcgctcctg tgctcccgtg tcgcgctctg ctcctccgcc gaaccgggga aacccaagcc 300 caagcccagg gtctccggca gccgcagggc gctgcttgcg gacaacgacg gcgacgaggt 360 gcccgccgcc aagccgggca agtcggccgc ggcggggaag accaaaaaga atctgctcgc 420 cggcgacgcc tcttccaaga accagaccag ggttgccaag gctaagaagc cagagtcagt 480 ctgccgcggc gaagggggcc gccaagaagc ccgctggcaa agcggctgcg gctgcgg 537 // ID DY239121; SV 1; linear; mRNA; EST; PLN; 518 BP. XX AC DY239121; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G22.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-518 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 51dc88cbd6416fbda12723e5577554cd. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 22. XX FH Key Location/Qualifiers FH FT source 1..518 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 518 BP; 106 A; 158 C; 118 G; 136 T; 0 other; gactgccaaa agcaactgca ctgcaaatgt aaacggctga aagttacacg gtccgttcca 60 cggataaccg tggcgcggaa gcttttatga attacaaaag gcgatttttc tcacaaaggg 120 aacttttgtg actttacacc gccccgagaa ccatccatcg attcaatttc aatcatgtgt 180 ttgtctcctg acgctgcccc ggcagcatca agggtcgcaa ccacagcaat ccgagcccta 240 gtagacctcg acgtgccatt gcttgttctt cttgagctgg gccagcttct ggtcccagct 300 ctcgcctctc cgctccgcca ccttcttaag ggtgtcctgg attccaggca tcatcccctt 360 caaaccgcag aagtagatgt gcgcgccgcc gtccaggagc ttgaagatct cgtcgctgta 420 ctcctcgatc ttgtcctgga cgtacatctt gccgccgctc ctgttcttct gctccctgct 480 gagggctttg tcgtacctga aattgtctgg atactgct 518 // ID DY239122; SV 1; linear; mRNA; EST; PLN; 571 BP. XX AC DY239122; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G22.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-571 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; a706695005cafbc05e8105b42eb76908. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 22. XX FH Key Location/Qualifiers FH FT source 1..571 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 571 BP; 95 A; 214 C; 162 G; 100 T; 0 other; ccagcgggag cgagcgggtc gtcccttccg ccacctcaag agctcactgc tggccctgac 60 tgaggccctc ggccctcctc cagtcccctc cgcggctccg cctctattaa acccttgtcc 120 cttcggagag actcctctcc tccttcaaat aaaccctccc ctccccggcc cgacgcccct 180 cgcccccccc ctcctgacca gccatggaga ccgctgctgc tgcgtcccgg gggggtgtct 240 cggcgccggt cttcggttcg gacggcggcg ccaggagctc cgggatcaag ggcaacaaca 300 atgttagctt cggcagcaaa tcatgggtcg gcggcacatt ggcctgagag agcatctgct 360 cggtgcggcc caggcgcgcc agcaaggtgc tctgcatgtc cgtccagcag gcgagcagga 420 gtaaggtctc cgtcgcgccg ctccatctcg agagcgccaa ggagccacct ctcaacacgt 480 acaagcccaa ggagcccttc acggccacca ttgtctcggt ggagagtctg gtcggcccca 540 aagctccggg ggagacttgc cacatcgtta t 571 // ID DY239123; SV 1; linear; mRNA; EST; PLN; 370 BP. XX AC DY239123; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G23.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-370 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 090aa4f2958a07341bf49250950c2c20. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 23. XX FH Key Location/Qualifiers FH FT source 1..370 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 370 BP; 89 A; 112 C; 97 G; 72 T; 0 other; gaagacaaca agaagaaatt cataaccatt tattcggttg tggtgtacga ggcaacacgt 60 acggcgctgc caccgctgac aatagaaaat acaacgacga cgaactcatg aacacgaact 120 gctctgctga gcacactaaa accaaaacta gattattggt tcgtcattat tactcccggc 180 cagccaccgg ccgaccgatg gatgctctac tcgatgccca gcctcttctt catcccgcag 240 acgaagtcgt agaccttctc gggcgggtag aggttcttgg cgacgctggg gcgctcggcg 300 cagcgcttgg cccaggcggc cagcctcggg cactccttct ccacgctgac gccgccgaag 360 cggtcgtagg 370 // ID DY239124; SV 1; linear; mRNA; EST; PLN; 446 BP. XX AC DY239124; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G23.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-446 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; e5cc1e2e2722b22cc19522d7d732fb5b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 23. XX FH Key Location/Qualifiers FH FT source 1..446 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 446 BP; 85 A; 151 C; 147 G; 63 T; 0 other; gctatgcatc gatccgccat tgctcaccgc acaggtgcac gctctcctcc cccacgcagc 60 taagtagcta acgccggagg ggagaacaaa aaaaggctcg acatggccga ggagaagaag 120 cgggccctgc agctgctgga cttcggggtg agccctttcg ggcagcgctg ccgcatcgcg 180 ctggacgaaa ggggcctggc ctacgagtac gtggagcagg acctgaggaa caagagcgag 240 gtgctcctcc gcgccaaccc ggtgcacaag aagatccccg tgctgctgca cgacggccgc 300 cccgtctgcg agtccctcgt catcgtgcag tacctcgacg aggcgttccc ggagggggcg 360 ccggcgctgc tccccgcaga cccctacgcg cgcgcgcggg cccgcttctg ggcggactac 420 gtcgacaaga agctgtacga ctgcgg 446 // ID DY239125; SV 1; linear; mRNA; EST; PLN; 528 BP. XX AC DY239125; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G24.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-528 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 4453dff66c57b50cf4117260c8d11048. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 24. XX FH Key Location/Qualifiers FH FT source 1..528 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 528 BP; 133 A; 127 C; 144 G; 124 T; 0 other; acgtgcatac gttggcgttg tattattcaa catgtccagt cgttatcatc catccatatt 60 ctatacatct atctatacct cacatctaac gcacgcaacc tttgtatata ggcaagtatg 120 gaacggtaaa ggtgagagca ccctggagta gctacagtta ttcctgttga gaattcccag 180 cttgaattct ggcaccgggc tgaaatataa ctgaacagct gttgcatcaa tcacgcgggc 240 cgtcaaatga ctgggatttc ctcaagttcc agtggagacc ctcatggatg ctgcggagga 300 aatcggtcgt cgagtccacc aggcaggctg tgggcacggg ccaaggagaa atgctgcaga 360 tcagggcgtc accgggcgac agctgctgtt cgtctttgcc gtcgaaggat gcccaggcat 420 tgcccctgct gttgaacggc acttgaacgc ggagggtccc gtattcaggc agaatcaaag 480 gtctgaatga caaaaagtgc gggcagattg gggtgaaaag gatccctg 528 // ID DY239126; SV 1; linear; mRNA; EST; PLN; 432 BP. XX AC DY239126; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257G24.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-432 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 4abb1620adc44b15d4d8525a36b842b1. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: G column: 24. XX FH Key Location/Qualifiers FH FT source 1..432 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 432 BP; 91 A; 126 C; 117 G; 98 T; 0 other; cttctccccc cttgcgaatc gaagaccagg tgcagttgcc cacccaagcc ccggaaccgg 60 gggccaagag cagtacaggg ggttggtttc tgcgacggcg aggacgcgaa gcggattcgg 120 atcgcgccgc cgtgccgctc gacgagctcg cagacaaagg gccgtcgtcc tccgaggaag 180 ttcagctcgc gccgtcggat tccaagttcc ggagcctcag cccttcgccg attccgctcc 240 caactcttcc gagcatcata tcattggtcc actcggtttc tgacgagagt gtaaatgtag 300 atgtcgcggc ctcacatcaa agtgaaagtg gatctatctc tactgttagt tcactagaat 360 cagagagggc agcatatgaa tttcttgctc aaactcctgt cgggtcaact gatgcacatc 420 ttgttgagtt tt 432 // ID DY239127; SV 1; linear; mRNA; EST; PLN; 450 BP. XX AC DY239127; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H01.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-450 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; c112c91fbfee37256a0517b0e0f104b0. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 01. XX FH Key Location/Qualifiers FH FT source 1..450 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 450 BP; 114 A; 110 C; 131 G; 95 T; 0 other; gacaacaata ggagtccatt cattcattca ttcattcatt catgttgttg cttatacttc 60 cgaaaacatt aatcaacgca aataaaaaaa acaagaggaa taaatttaca ggcatagtat 120 acgtttagga acgacgactt ttgtgtagtc tgatcgattt gaagccaaaa caaaagcatg 180 attacgtgac gaggatgagg ccctcggtga tgagcgagtc agcgatgacc tggttggccg 240 cctcggacgg gtggacggcg tcccaaaaga cgtaggacgt ggcgttgggg cacgtcccga 300 cggacttggg gttgcagagg agcacggtgg tctccaccgt gcccgtcccg cagcatcccc 360 gccgggcctc cgcgaagccc tgcgactggg ggtccgtggc caggtcgtac agcggcgtgt 420 aaatgtccaa gacggcgatc ttgagggccg 450 // ID DY239128; SV 1; linear; mRNA; EST; PLN; 510 BP. XX AC DY239128; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H01.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-510 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 77241b116479e59838ed51eeaab5e792. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 01. XX FH Key Location/Qualifiers FH FT source 1..510 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 510 BP; 92 A; 165 C; 154 G; 99 T; 0 other; gtgcgttgcg tcgggagtgt ttgtgtttca cagctgagag tctgatactg aaaaagatag 60 aggtgatgat ggttctgcag gcgcagggga cgcgggtgcc gctggtcctg gcgctcttcc 120 tcctcctggc cggttgcggc agcgaggcgc acccgctggt gccggcgctg tttactttcg 180 gtgactcgtc gggggacgtc gggaacaacg actacctcca caccatcatc aaggccaact 240 tcccgccata cggcagggac ttccccaacc acgtcgcccc cgggaggttc tgcagtggca 300 agctggcgac cgacatcacc gccgacacgc tggggttcac tacctaccct gccgcgtacc 360 tcagcccaca ggcgtcgggg cagaacctcc tcatcggcgc caacttcgcg tccgcggggt 420 ctgggtacta cgaccacacg gcacttatgt atcacgcgat cccgctgtcc cagcagctgg 480 agtacttcag ggagtaccac acaaatctgg 510 // ID DY239129; SV 1; linear; mRNA; EST; PLN; 402 BP. XX AC DY239129; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H02.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-402 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 5d658ea16bf49a949fdea0ed0428a851. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 02. XX FH Key Location/Qualifiers FH FT source 1..402 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 402 BP; 146 A; 77 C; 81 G; 98 T; 0 other; gcagttgaaa agaactggct tatacttttt tttagaagtt tataaaatta caaccataaa 60 gatcagcact aacattaatg actttgctaa cataagagtg gcataccatg cgtgccatac 120 aaactacagg tacattcata catagcataa cttacagcac atgatacaag ctaccgagca 180 cgggcaatct taaatatata aaaagacaat attgaaggct cccatatgag tgggggctga 240 agaaggagaa aaaccagggc aggctttccc cgcaaatacg gagaagctac ttcgaaccag 300 tgaccttatg actcagtgat cttaaattta gaagaagaaa aaagagcaaa gcttattttg 360 cccaggattc catccaaaat gcagtttctg tgctggtagg gg 402 // ID DY239130; SV 1; linear; mRNA; EST; PLN; 472 BP. XX AC DY239130; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H02.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-472 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 8dab1620c9775b8f66c8d45e58fa4952. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 02. XX FH Key Location/Qualifiers FH FT source 1..472 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 472 BP; 92 A; 179 C; 133 G; 68 T; 0 other; caaggcttcc tcccaccacc tccagtcctc cacaggacca ccacctccag tcctccacag 60 gaccaccacc tccaccactc ctctctcaaa acacgcgcaa accgctcgaa ccctatctgg 120 gggcgggagg cggaggtggc tccgggatga cgcggcggtg ctcgcactgc acccacaacg 180 ggcacaactc gcggacgtgc cccacccgcg gggtcaagat cttcggggtg cacctcaccg 240 atggctcggc catccgcaag agcgcgagca tggggaacct ctccctcctc tccgcgggat 300 ccaccagcgg gggcgcgtcc cccgccgacg ggcccgacct cgccgacggc ggcgggggct 360 acgcctccga agacttcgtc caggggtcgt cctccgccag ccgcgatcga aagaaaggtg 420 ttccttgcac tgaagaagaa caccggaggt ttttgctgga attacaaaag ct 472 // ID DY239131; SV 1; linear; mRNA; EST; PLN; 588 BP. XX AC DY239131; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H03.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-588 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 373fe5099833ae3d219a1e95288457d6. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 03. XX FH Key Location/Qualifiers FH FT source 1..588 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 588 BP; 118 A; 181 C; 176 G; 113 T; 0 other; agcatcccca gagtcagcag ccacatcaac cacagaatca gtcacaatct ctagctcgcg 60 catggaaacc accatctcat cgacatgatc aatgaacgga ccaccatcgc ccccgctggt 120 atgagctggt atggcgcaca agatgcgccc catggccctg ctgacaggga cagtatcagc 180 actggacccg gcccggttcc ctggcaataa tggcacagta gcgatgttga gtctgtccgc 240 gaggtggcag gccttggtga tgtagtcgtc tgcttgccgg agccattgct gctgcttgac 300 tgccgcgtcg tgtagccaag cgcgcgcagg ggccaagagg ttctctactg ggcgctgcac 360 ctgatcctca agaaggtggc tgaggtcggt gcgcagggta gcagcttcag tggcgaacat 420 cttctgcagc cccacattgt cccgagccct gagctcctcc aaagatggga gtttgagctc 480 gaacccgaca gggacgtgca gctgttggtg tcgacggccg tcgccgccgc cgtgactgga 540 acggccgaac tccctacgtc ttggggacct ggaacgtgtg cgcgtcca 588 // ID DY239132; SV 1; linear; mRNA; EST; PLN; 536 BP. XX AC DY239132; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H03.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-536 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; faf803d63b44f3babc88a446707a10b8. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 03. XX FH Key Location/Qualifiers FH FT source 1..536 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 536 BP; 90 A; 212 C; 137 G; 97 T; 0 other; tgcaattcga ggtaaaaaag tatatgcccc cgccgccgcc ccccaccccg tcgccgcctc 60 ctcctcccaa cccaccgccg ccaccgccga ttgccctccc taaccctgct gcgggccagc 120 cccagcgtgc gtcgcctagt ctattcaaac atctcttccc gcacctcgtt tttcccggag 180 actccattgc cccagctgcc gtcgggtccc gtcagacatc tgctccaaat ccggcgccct 240 cctgccatgc ccgccagagc cttggtcttc ccgttccaga tctgcgtcca ggcgggggtc 300 cgggctcgcg ccgtgcgggc tcgtacgcgg aggctgtcat gggcggacca acggcattta 360 ctcgcccaca gccactctgc ttcaattcgt gtcccatcgg gcggagacag agcgctgacc 420 cggtgcctcg ccacccctcg agccgtgcag cgccgcgcac ttcaatggct cccctcaccg 480 caaatccgga agaagatttg ggctggcaca cagtcaaggc aaaatacggg tggaga 536 // ID DY239133; SV 1; linear; mRNA; EST; PLN; 428 BP. XX AC DY239133; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H04.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-428 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; e6236b3ceed17d56ffe8a94291f3cf7b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 04. XX FH Key Location/Qualifiers FH FT source 1..428 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 428 BP; 118 A; 115 C; 95 G; 100 T; 0 other; gagattgtac tcgctatgct aatcttcata tccatgaaca gtgctaccat ccagatccag 60 tggctaatgg caaggccagt cagtacaccg gcaataagtc ctgccgtgct gtgctggatt 120 attgctcata cagcagcaag ttccagtttt aatacacaca catggataca tcaaggacat 180 cgcagatgac tggtagccca attccttcat tttcctgatc gcgacaccag ggagagagtt 240 catatatgga agacgaaccg gcgatttttc aaaacctctg atcatggatg gttcctaata 300 tatgcaagac gaccaaacac ctggcccaga cgcccagtct aggactatga gcaagcggca 360 atactgaggt cgctgcgcta aaaggacacg ccggatcatt cctcctgctc gatcatcctc 420 cggacgtc 428 // ID DY239134; SV 1; linear; mRNA; EST; PLN; 461 BP. XX AC DY239134; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H04.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-461 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 21782db94c3225b78486ab63a648e640. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 04. XX FH Key Location/Qualifiers FH FT source 1..461 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 461 BP; 87 A; 149 C; 145 G; 80 T; 0 other; gtcaaaacaa aacgaagcat ccacgtttgt cgctcccacg ctggcaatgg ccgccgccgc 60 atctcccgcc accgccgccg ccgtccgcga agcggcgccg gcccacagac aaattgaaat 120 ccgtcggggt catggtccgg cccctttccg tatcggtttc ctcgccaaac cgtcctcccg 180 gggggccgcg atctctgtgc cgcctgggcc tcgccgcgcg gctccggggg cgtccgcggc 240 cagggagagg gtcacggaag aagaggagag gcccgtgtcg gtggagctgg aacccatcgc 300 cagcgagcag catctggact gggtccttgg ggaggcgcgg cagctccagc tccccatcgt 360 cctgctgtgg atggcgagtt ggtgcaggaa atgtatatat ctgaagccta agcttgagaa 420 gttggcagca gaatatcatc caaaaatccg gttctactgc a 461 // ID DY239135; SV 1; linear; mRNA; EST; PLN; 524 BP. XX AC DY239135; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H05.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-524 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 44af4d813d4fd5ddab7251655db1eeb6. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 05. XX FH Key Location/Qualifiers FH FT source 1..524 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 524 BP; 153 A; 142 C; 92 G; 137 T; 0 other; gaaaaacata tatcgtgatt gatatcacaa tgtatttgtt tcttttgata aaagtcacat 60 gactctcgat gaacatgacg aatgtacaaa tcatctttat gctccacgat gtcactccgt 120 atatccaagc ataatcgcat gaacatgaat ccctacaact ttagatacac aatcttaagt 180 gctacgcagg tcaacaaacc caagcaacgg tcacccctgc cattgctaca atggacaccc 240 tactacaaaa ctgcaagagc agcacaacca agaaatcttt tctggggatc ccgtgactga 300 aaggacaagg ctccacccgg acaagcgtct ctattcatgg agttaacaga agccatcctc 360 ctcagatgct ttgcatttgg aaaatcacag gtgatagccc tgaattgagg tgttaaatct 420 tcccagctgc ttctgtacgc gcccttagtg cctcggtgcg tttactcctc tccatttctt 480 ccaccaacca gtcaacgcca tgtttgatcc ccgtcccatt gaag 524 // ID DY239136; SV 1; linear; mRNA; EST; PLN; 559 BP. XX AC DY239136; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H05.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-559 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 495e48d4fb200eb428f90b8ed4404b92. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 05. XX FH Key Location/Qualifiers FH FT source 1..559 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 559 BP; 125 A; 148 C; 147 G; 139 T; 0 other; gccctccctc cccccgcgac cgcgattctg cgagcacgac aagacccacg gacccacctc 60 gatccagatc cagccaactg cgcgcgtcgg cgttacccct tcctcctcga cgccactgcc 120 ggggagcggg gatcatccgc ctgaaccggg tcgaccgctt cgttccttgg gggggggtag 180 ctggttgggg gagctcaaaa ccttacactt acaactatgt tctccttgtt ctatggtcta 240 tggaagcacg tcttcagcaa ggcagagttc catgtgctta tcctcggagt tcataagggt 300 ggcaagacga ccttgctaga aaagttgaag tcgatctact tgaagggaga agggcttccg 360 catgatcgta ttgttccaac agttggcctt aatattgggc gtattgaaga tgcaaatgta 420 aagcttgttt tctgggatct gggaggccag cctggcctac gaacaatatg agagaaatat 480 tatgaggaag cccatgctgt aatatatgtt atcgactctg cttctgcatc atcatttgag 540 gatgctaaat ctgctctcg 559 // ID DY239137; SV 1; linear; mRNA; EST; PLN; 556 BP. XX AC DY239137; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H06.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-556 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 89a1d997245c1812311639f51ecd0945. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 06. XX FH Key Location/Qualifiers FH FT source 1..556 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 556 BP; 158 A; 166 C; 101 G; 131 T; 0 other; gaagcatgat ggttacaggt tacaccacca ctacagtgtt ttagcacata ccaaatacaa 60 ttccaattat tttggagtgc ataccgtctc catcaatgaa ctcagattgc agtatgaaga 120 tccaccctca ttgactgctc tgcgagaagc ctccttcaac tcaagagcac gttccctcag 180 cgcctttgtc ccagtttcat cagaagacat cagcttcttc acagctcgtg caatatcctc 240 tctcccgatc agaccaccct tgctagccca atccctaacg gcaaggccaa tcttccactc 300 ttccacaatt aatcgcccat caatcggttg atcaaaaaac agtggtaagg caagcatagg 360 cacaccagcg aagacgcctt caagtgtcga gttcatcccg caatgggtca tgaagccccc 420 aactgaatga tggcacaaca cctcaagctg ctcacaccat ggcagtatca tcccattatt 480 gatgccaaag aagagttctt gcacccgagg ggattgttct cgaagaatcc acaagaacct 540 gacctcactg gctatt 556 // ID DY239138; SV 1; linear; mRNA; EST; PLN; 439 BP. XX AC DY239138; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H06.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-439 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 6b9818bfecad937547b02408fbfbc07c. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 06. XX FH Key Location/Qualifiers FH FT source 1..439 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 439 BP; 63 A; 180 C; 134 G; 62 T; 0 other; ctgaccaaca gccaagccgg gaggcacgct tgagagccac acactgagac ctcacgcgcg 60 gatggccgcc gccgcgggcg ggaccggcgg ctgcggctgc cactttgtag cggtgccgtt 120 cccgggccgc ggccatgtca acgccatgat gaacctgacc cgcctcctcg caccccgggg 180 cgcggcggtc accttcgtcg tcaccgagga gtggctcgga ctcatccgct cctcctccgc 240 cgccgccgct gccgagccgg ccggtatccg catccgcacc atccccaacg tcatcccttc 300 agagcacggg cgcgccgcca accactcagg cttcctcgac gccgtcgcca cagagatgga 360 ggcgcccttc gaccgccttc ttgacgggct cgaaggcccg ccgcccgcgg cgctcgtggc 420 agacgcctac gttccctgg 439 // ID DY239139; SV 1; linear; mRNA; EST; PLN; 534 BP. XX AC DY239139; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H07.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-534 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 0e8bf1b9fb505e8d436b2b2d02a1514d. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 07. XX FH Key Location/Qualifiers FH FT source 1..534 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 534 BP; 90 A; 237 C; 118 G; 89 T; 0 other; gcgagagaaa aaaatcccaa tccatccacc ctcgccgcaa gatcgccggg cgccgccatg 60 tcgactccag ccgccgccta ctacgccgcc gccgcgcgca agcaatactt ttgctaccaa 120 tgcaaccgca ccgttctcat cgcggcctcc gccgccgccg ccggggagct ctcgtgcccc 180 gagtgccacg gagacttcct cgaggaggtc accgtccctg cccccacctt catcccggtg 240 cccttcccgt tcccctttgc ccccaccacg atccccgccg ccagcaccac cccggccccc 300 gccccaacag ccggctccgg tgggtcccct tccttatcgt cctcgtcctc ttccgccgag 360 acctcccttt cccagcccaa agacatctcc tccatcctca actccttcgt gggactagga 420 gaacaaccca tcagggtcgg cggctctgcc tcgcgcgccg ccgccggcac ggccacccca 480 gagaacgagc cagagccctt cgacccggtc atgttcttcc agaattacat ccat 534 // ID DY239140; SV 1; linear; mRNA; EST; PLN; 637 BP. XX AC DY239140; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H08.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-637 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 5bfa7b36457f86157a02396c5a0586af. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 08. XX FH Key Location/Qualifiers FH FT source 1..637 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 637 BP; 169 A; 169 C; 137 G; 162 T; 0 other; agataaagac aaatacagcc ttgatttcaa tggactctag caaattgtca aagtgatcac 60 tgagtaatta atatgcaccg gtggacttcc cactagtacc gaacagctaa gttggcatca 120 aagtgttgct aaaacactgc gttatcacag atcaatgaag acacgcaggc cgtcatgagc 180 tagctgcact ggtattcttt ccctgcatga ccatcttgct aacagctggt tttctctgtg 240 atgttcaaac tcgtggttca tcccaatcag cagagctcgt ttagggcgta tcctcttgac 300 agcgtcaagg ctctggctca gagtcaaatg gcaactgcga ccgtctcccg ccccatgcaa 360 cctgtttgtt tctagtatga gtagatctag ctgcccttgt ccggtggcgg taccagactt 420 cgaaatcgcg tgctcagttc taggtagaat tcttgagaca tcagacaagt atgcaactct 480 cgctttcctc ccgaacaaga agcctaaaca gacatagtcc tctccatgca tgacgggcaa 540 cggccaaaac tgaagctccg atgctacaaa tggcttgtca catcaccctc gattatcgtc 600 caatggagct gaacaggtct ggcgaaatct cacctcc 637 // ID DY239141; SV 1; linear; mRNA; EST; PLN; 468 BP. XX AC DY239141; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H08.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-468 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 34ff60f1af50644b676378a994fc3d0d. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 08. XX FH Key Location/Qualifiers FH FT source 1..468 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 468 BP; 94 A; 160 C; 118 G; 96 T; 0 other; agaagccaag aacctgtgac gcgagccatg gactctggtc cggtgcccac ccccccaaca 60 ggaggttccg cagcggtggc ttcgtcgtct tcatcactca tctttctggg caccgggtgc 120 tccggcgcgc tccccgacgc gcgatgcctc atccagccgt cagaccctcc gtgcaccgtc 180 tgctccacgg ccctctccct gccgccggac cggaacccca actacaggtg caacacctcc 240 ctcctggtat attactgcca cgaagatgga gctcacaggt atatcctgat cgatgtcggc 300 aagaccttca gagagcaagt cctccggggg ttcgtgcacc acaaagtccc gtccattgac 360 tcggttattc tcacgcacaa acacgcagat gctgtcttag gcctcgaaga tctctgggtg 420 gcactaccga gctgtcagaa aaacggtagt gccaaagttc cggttttt 468 // ID DY239142; SV 1; linear; mRNA; EST; PLN; 549 BP. XX AC DY239142; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H09.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-549 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 85c90afc626370064f26e9aeb96e3720. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 09. XX FH Key Location/Qualifiers FH FT source 1..549 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 549 BP; 117 A; 150 C; 201 G; 81 T; 0 other; tccacggcgt tctccctggc ggccggcggc aagaaggtgg ccatgtgcgc cagcgccggg 60 atgatgtaga cggtgaagct gaccagcagc gagcccacgg tggagttgat ggggccgaaa 120 aacgggaaga cgacggccag gaaccagatg ggcgccacga tgggcagccg cgcggcggcg 180 cgcagggcca cgctccccgt ctcgtgcacg ccgatcagct tctcccacac gaagtagagt 240 ggcgtgcacg cgaacccgaa cgtgatgaac tggtggatga gcatgaagat gactgcggcg 300 tcgcggaagc cggagcgcgg gaggagggcg aaggcgttgg agtggtccag gagcatgtcg 360 ccgaaggccc agtagacggc ggaggcggac ggcagcgtca gcgtcagcac gtacagcgtc 420 gccaccaggt agatgagctt gaacttgtgc ggtttccaca tcgcgtgcat gatctccaca 480 gtgacagcat ggcctccaaa ggtgtagagg atgttggtgg caccggtgaa atagagcacc 540 atcttgctt 549 // ID DY239143; SV 1; linear; mRNA; EST; PLN; 452 BP. XX AC DY239143; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H09.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-452 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; c4698ecac92adfd6db9e85ed98fc9e54. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 09. XX FH Key Location/Qualifiers FH FT source 1..452 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 452 BP; 99 A; 127 C; 155 G; 71 T; 0 other; cagagagggg agaaaggcta gttatcagga aagatccatc cctcaggaac aggaagaagt 60 gaggcgacag ctctgcagga tggcatcgga gaaggtggag acgatcgtgg caggaaacta 120 catggagatg gagcacgagc ccgggggcgg cggagaccat gaccagcaac ccagcggcgg 180 cgcggcgtcg tccatttcat ccagctccag gggcggcggc aaaaaaaagg ccctgtcgag 240 cctgttctgg cacggcgggt cggtgtacga cgcgtggttc agctgcgcgt caaaccaggg 300 ggcgcaggtg ctgctgacgc tgccctactc cttctcgcag ctgggcatgg catccggggt 360 ggtgttccag ctcttctacg gcctcgtggg cagctggacc gcctacctca tcaacatcct 420 ctacgtcaag taccgcacca ggaaggagcg cg 452 // ID DY239144; SV 1; linear; mRNA; EST; PLN; 411 BP. XX AC DY239144; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H10.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-411 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 976fddc547d0c1cd431b8ae8dd94956c. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 10. XX FH Key Location/Qualifiers FH FT source 1..411 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 411 BP; 101 A; 117 C; 109 G; 84 T; 0 other; aaagtactag ttgctgctag ctggctatat tttgccagta attgatagct tgatttatgt 60 acatatgatt aagagggcgc acatagaaat cagcaattca gcacagcaat gacacgctac 120 gatgagtagc tagctagaat taatctgccg acgacgatgg cataccatac catcgattac 180 atcagtccat acaggctaaa ccactcgacc acgctgctgt cagcgtaggc atagtcagga 240 acatagagca ggtcgtcgtc ccccaggtga tcgccgccgc cgccgcctcc tccgggctgc 300 tggcaggtgc cagtcgagaa cgatgagctc gggctcccgc tgcagacggc acgggccatg 360 acgtctccgc cgtcaccgga gaccgcgtgg ttgcccgcgg atctgaaacg c 411 // ID DY239145; SV 1; linear; mRNA; EST; PLN; 390 BP. XX AC DY239145; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H10.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-390 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 1e21cc9862fdc7f6ef15cad687995e4d. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 10. XX FH Key Location/Qualifiers FH FT source 1..390 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 390 BP; 70 A; 144 C; 106 G; 70 T; 0 other; gcgccggtgg tcctcgctcc ttgatccact cctcctgcag ctcctcctcg aacaggacgt 60 ctcctctccc cagcctccct ctctttctct gtcgtgggtc ctctccccag cagcctccct 120 ctctctttct ctatccgggg ccctctccag ataaccagac acaggggtca cgcttgacaa 180 ggagagaaaa aggcgttgca gcagctgatc cgcgcccgcc cggccgggag cgcaggaaaa 240 ggatggaaca atacgagggg ctctttcctc ccgcctacgt ggactcctcc tcatccctcc 300 tcctggtgcc caacgccaac ggcactgcgc aagaagagag accgagagcg cggcacaaga 360 ggcgtcgagc agcgaggtgc ggcggcggcg 390 // ID DY239146; SV 1; linear; mRNA; EST; PLN; 543 BP. XX AC DY239146; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H11.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-543 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 62ff6e1e39ee34382abf44a1dbeab144. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 11. XX FH Key Location/Qualifiers FH FT source 1..543 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 543 BP; 147 A; 108 C; 114 G; 174 T; 0 other; aacacaagaa atgtacttgt attgccattg ctgacagaat acttcattag tacagttgat 60 atgaataaag aatcttcagc cacaagtttt ttttcccgtg gttaaaactc cagcaatgag 120 gattaattct tctcacattt ccattttgag gttgtctagc aagcagccac ctgcagggaa 180 agcagtagct ggattcagtc acttctgttg cagttaaatg tacagggaca tatgcacagt 240 tcatacaagt caaaatttta tttgatgtgc tcatttttgg tggagttatt tgatcggcag 300 ccagtttgag ttttctgtga tggcacgtta cggccattat gaactctgag ctttaaacat 360 gttcaggttg gcgttggggg gttcattttt accctgatct aaccactatt gtgttctttc 420 atttcacaag aggcggcaaa gtaacttctg gcttgttctt gaggaagcag ctactcactt 480 tctaccgact gaaattgtgc atcaaatccg ctgaacaaat tctgggagcc aaaatcccag 540 gca 543 // ID DY239147; SV 1; linear; mRNA; EST; PLN; 486 BP. XX AC DY239147; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H11.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-486 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 2756821afe5673e5f55ac3bde829ae9d. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 11. XX FH Key Location/Qualifiers FH FT source 1..486 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 486 BP; 87 A; 152 C; 176 G; 71 T; 0 other; gtcccgcact ccttccctta tccttaccac tcggagttga tcccgtctcc gggggaagca 60 gctggtaata atggcggcgg tgccagggga ccgttgagag cctctgccag gggctgctcg 120 acctcgacga cgacaagttc ggtgcagtgt gcagcgcctt cggctacctc caggagtggc 180 cggacctggg tgccgccgtg tgcggcgcca atctcgccgt gccgcctgcc gtggcgaccc 240 cgagcgccgg cgacggcaac gattcctcct cttgcagcgt cagcggcggg ttgaggaaga 300 ggaggccgga cgcgtacctc gatgccaagg gtggtgcgaa cggcatgaac aagagaccca 360 gagggaagca gcagccgtct ggcctctctg atatcgcggc ggcgcccggg aaagggaagc 420 aggagaggcc aaaggctggc accaaaaaga aggccgaggc tgcggcggcg gcggcggctg 480 ccggcc 486 // ID DY239148; SV 1; linear; mRNA; EST; PLN; 512 BP. XX AC DY239148; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H12.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-512 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 73d117204d90da57e4224625b433e350. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 12. XX FH Key Location/Qualifiers FH FT source 1..512 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 512 BP; 112 A; 180 C; 145 G; 75 T; 0 other; ctccggtccc caacccctgt cgcaccgcag ccgccggcga tgggctgcct caccgacctc 60 gtcaacctca acctctcgga caacaccgag aagatcatcg cggaatacat atggatcggg 120 ggatctggcg tggatctcag gagcaaagca gggaccctct ccggcccgga gaccgatccc 180 agcaagctgc ccaaggggaa ctacgacggc tccagcacgg gccaggcccc cggagaggac 240 agcgaggtca tcctgtaccc gcgggccatc ttcaaggacc cattcgggag gggcaacaac 300 atccttgtga tgtgcgattg ctacacccca gccggagagc caatccccac caacaagagg 360 tacaacgccg ccaagatctt cagcagccct gaggtcgccg ccgaggagcc gtggtatggt 420 attgagcagg agtacaccct cctccagaag gacaccaact ggccccttgg gtggcccatc 480 ggtggcttcc ccggccctca gggcccttac ta 512 // ID DY239149; SV 1; linear; mRNA; EST; PLN; 515 BP. XX AC DY239149; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H13.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-515 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 3dd7dae8cd3550f92f075ebddb4d57f8. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 13. XX FH Key Location/Qualifiers FH FT source 1..515 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 515 BP; 104 A; 171 C; 130 G; 110 T; 0 other; ccctctctct gctcctgcag gatgcgatgg tgattccgcc gcggactcct tccccgccgc 60 catcgtggtc gcgctctgta accgagaccg ttcgggggtc ccaccatttc accgtagggg 120 gctactccct cgccaagggc atgggcccgg gccgctacct cgccagagac gtcttcgccg 180 tcagaggata ccagtgggcc gtctacctct accccgacgg aaagaacgca gaggacaact 240 ccaactacgt ctccgttttc gtcgccctcg cttcagacgg tatcgacgtc agagccctct 300 tcgagctcac cctcctcgac cagtccggcc gcgggtgcca caaggttcac tcgcactttg 360 accgctcgct caagttcggc ccatacaccc tcaagtacag gagatccatg tggggttaca 420 agcgcttcta caaaagaaca ctcttggaag aatctgattt cttaaagaat gattgcctag 480 tgatgaactg cacagtaggt gttgtcaaga accgt 515 // ID DY239150; SV 1; linear; mRNA; EST; PLN; 428 BP. XX AC DY239150; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H14.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-428 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 43b27b2ebfb8b9050aaf2e3b4fd7a779. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 14. XX FH Key Location/Qualifiers FH FT source 1..428 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 428 BP; 70 A; 129 C; 141 G; 88 T; 0 other; ggatctcatc gaggcgacga cggcgatgtc aagggaggcg gcgaggacga ggagccgggt 60 cctggtggtt ggcgccaccg gccgcctcgg tggaagcatc gctcgggcca ccctcgccgc 120 gggtcacccc accttcgcgc tcgttcggcc ccaccatttc gcacgccccg attcccccgt 180 gcttgggccg ctcgtacccg ccggcgcctc tctgctccgg gggtcactgg aagattactc 240 gaccctgctc gaggcggtgt gtcgggtgga cgttgtcctc tgcgcggtgt ccacgaagcg 300 ggtgctcgag cagaaccctc tgattcgggc tataaaggaa gctggatgcg tgaagagatt 360 tattccggca gaatttggag catatccaac aaaggttcaa atttgtggcg tggattatgg 420 cttctatg 428 // ID DY239151; SV 1; linear; mRNA; EST; PLN; 496 BP. XX AC DY239151; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H15.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-496 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 7fb8d034ccc0725fc2aaea92c17ee1c9. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 15. XX FH Key Location/Qualifiers FH FT source 1..496 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 496 BP; 131 A; 139 C; 146 G; 80 T; 0 other; ctcttggctg atcaaatgtt caaataaaac agcagtgcca agaacaaaaa ggatcagcag 60 ccagatgaga ggtaaggtac tgctgatacc ccatcgcctt agcttagctt aacccatgcc 120 ctaacatctc gtacccatag cataacagga acacacagtg caactactac aacaacaagg 180 acaccagcag caaattgtct gtcacgtcac gtactaaaaa gaacaacaac acatcaaatc 240 ctactggtgg cacaacttga cagagggtct agaacgagct aggcgacagc tctggcacga 300 gcaggaggag gtagccggcg agcagcaccg cgagtgcagc accggcggtc gccgccgagg 360 aggagacgcc gcgtccggtg gtcgcctgca gcgcgcggga gtcggtcgtc gccgtcgagt 420 tgtccttggc tggagcggca gtggagccct tgtcgccgcc ggggctgaag acggcggcgt 480 ctttggagcc cggggg 496 // ID DY239152; SV 1; linear; mRNA; EST; PLN; 612 BP. XX AC DY239152; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H15.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-612 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 2ee9ea36613952554c2984be268e5174. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 15. XX FH Key Location/Qualifiers FH FT source 1..612 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 612 BP; 102 A; 230 C; 204 G; 76 T; 0 other; caagtcacac agccacactt gaaccgctgc ccgcagcgga gggagcttgc acgggccaac 60 gcacacataa cacaagctcg tcgtcgatgg cgcggtgggc tgcggtgctg gcgctggccg 120 cggccacggc catcgccgtg gcgtccgtgg cgggcggcga catgagcgcg gacaagacgg 180 agtgcgcgga ccatctgggg ggcctggcgc cgtgcctgca gtacgtgcag gggcaggccc 240 gcgcgccgcc gcccgactgc tgcggcggcc tgcgccaggt gctgggaaag agccccaagt 300 gcctgtgcgt gctcgtcaag gacaaggacg accccaacct gggcatcaag atcaacgcca 360 ccctcgcgct cgcgctcccc aacgcctgcg gcgccacccg cgccaacgtc tcccactgcg 420 ctcatctcct gtatattccc ccgggctcca aagacgccgc cgtcttcagc ccgggcggcg 480 acaagggctc cactgccgct ccagccaagg acaactcgag ggagaagaca gactcccgcg 540 cgctgcaggc gaccaccgga cgcggcgtct cctcctcggc ggagaccgcc ggtgctgcac 600 tcgcggggct gc 612 // ID DY239153; SV 1; linear; mRNA; EST; PLN; 451 BP. XX AC DY239153; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H16.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-451 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; b30de3e558cb7bf9f70d5ce176ed1147. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 16. XX FH Key Location/Qualifiers FH FT source 1..451 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 451 BP; 103 A; 118 C; 135 G; 95 T; 0 other; aaatctggga cgatccttgg gatcgattga aataacagat aaatcaacag ttaattacat 60 attgcaagtc cttacaggta gaattagcga aagcttgaaa tactagcaaa ggcacgtgcg 120 aacgtacgtc aaaatttggc cacgtacaca tatataggcg gaagttaaat caaatgcatg 180 ccatccatgg tacgtacggc cggtcaggtg ccgcgcgcgc gctcatcgtc acaactccat 240 cagaggacag agatccggcc atcaggggca tgccgggttg tgtcccgggc caccgtagta 300 gaagtgggtt ccccagcctc cgcgggcgga gtgctggccg tcgtcgtcgt cgtcgtcgtc 360 gtcgtaggcc ttgcgtatgt cgtagcaggc ggggtcctcg gccagcgtct ggatggcgtc 420 gagcgccacc tcggccaggc tgttgtcggc g 451 // ID DY239154; SV 1; linear; mRNA; EST; PLN; 550 BP. XX AC DY239154; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H17.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-550 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 4880d5677cbea2b23a3a3efc24e973af. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 17. XX FH Key Location/Qualifiers FH FT source 1..550 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 550 BP; 156 A; 124 C; 122 G; 148 T; 0 other; cagcaaatgg ccaaagattc atcattaggg cattagaatg ctgaaattgc ccatcaaatt 60 acacaacctt aatagcagcc aaaccggcaa ccgtttcatc ccatatcatt agcaaccacc 120 gggcaaattg taaatcaacc aagtctgctt gcactcgatc tcggtggttt acagataact 180 gggagatcag actaaatata taaaatatag gaaccaggtg cggaagctgt ttccgagtca 240 ccatggatgg acatgagttg ctgccatctt tagaagaatg tcccttccgg aggctgaact 300 agcttgcggt tctgtggcga tgtcatctct ttcttgagat gggttaggat gtattccatt 360 gagtaatcac gctgccagtt acccagaacg ctgaacttct tcgggtcaac cactccagtt 420 tcatgattaa cgcatgttaa gtttattctt gaatgaaatc gaactgatgg tggcttctca 480 gggtagtcct tgtcgcagaa caacttcagc tggtagatgc gaccctcatg gacggtcttc 540 aagtaagaaa 550 // ID DY239155; SV 1; linear; mRNA; EST; PLN; 461 BP. XX AC DY239155; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H17.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-461 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 8d6965e763f01f97004cfb472fa036b4. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 17. XX FH Key Location/Qualifiers FH FT source 1..461 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 461 BP; 103 A; 89 C; 112 G; 157 T; 0 other; cggaaacccc aaacccaacc tctttttttt tctcggcctc taccgccccc tcgatcggtc 60 ggccggtcag ttatgacgcc gggaagctcg gccgccggat ccggtgtcgt ctttcctcgg 120 aatttcgggc tcctaaaaga gtttgaacgt ggagagaagg gttttggata tgggacagtg 180 agctatggag tggatgacgc atatgacatc tacgtgcgat cgtggagtgg cactattatt 240 ggccctcata gtgtgagatt attgtttctt tattgaatct gttatttttt tttctttttt 300 ctcacagtga gcctgagtaa taaaaaaaaa cttggtgcaa tttttccaag atcttgaagc 360 cacttttttg ctgttgtttt gtattatttg agaagtactt ggtgttggtg aaacgtgtca 420 tttgaaattt tgtagtagta cagaaaattt cgtccgaagt t 461 // ID DY239156; SV 1; linear; mRNA; EST; PLN; 462 BP. XX AC DY239156; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H18.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-462 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 56f0e03c4858030d0aad56425845f6bb. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 18. XX FH Key Location/Qualifiers FH FT source 1..462 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 462 BP; 101 A; 117 C; 132 G; 112 T; 0 other; ggcatgatcc gaaaacaagt cagcatgtaa tatccaaacc cttgtttagt ttgtaataag 60 attgaggcag acggccactg tacacacacg atacattcgg ttggttacac ggcagggttt 120 ctgcaagtgg aaatcagcag ccagcagcct tgaggaagtt gtcgtagggg ctcgacggtg 180 gcagcctcag gaacggctgc tggacgtcgc cagcaacctg tgcctcgccg gcactgatgc 240 tctgcaggag gaacgggttt gtgctctgct tgtcggaggt atgtgctgct tcagcgtggc 300 taacctgatt gtcaggcacg acgaccatgg ccgcttgctt gttttgcttg aagctcatga 360 gcagggacat cttgtggtgc tttcccatcg aaaaggcctc acagtggcta gtcatctcct 420 ggaatgggat gtctccggcc attgacacca aacattgccc tc 462 // ID DY239157; SV 1; linear; mRNA; EST; PLN; 367 BP. XX AC DY239157; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H18.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-367 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 71035c8a9686bc13cdba080449ccab37. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 18. XX FH Key Location/Qualifiers FH FT source 1..367 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 367 BP; 44 A; 114 C; 153 G; 56 T; 0 other; ctcgtgctcg tgcgcgcgcg tcgcctccca ccccttcgtc cgccgttgcc gcccgcattc 60 tcccttccgc cgtggtgggc gcgcggcgac gggaagagag gcaggagagg tcggaagcgg 120 cggcggaggc gggcccccct ccgtctactc ttactcacgc acaccagggc gcccgcgagc 180 tagcgcccta gcggagcgga ggtcgaggcg ggagaaaggc ggttggggct ccccggtggg 240 cagggggggg gggggggggg tcaaaggcgg aaatgggtct ccacggcacg ctctcggggg 300 gggtgtcaaa aaaggtgctg ccggcgtggg gggggttctg gtgcctgtgc ctttctgtgc 360 gtccccg 367 // ID DY239158; SV 1; linear; mRNA; EST; PLN; 431 BP. XX AC DY239158; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H20.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-431 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 71597ad31440475343ce6055edf0a283. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 20. XX FH Key Location/Qualifiers FH FT source 1..431 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 431 BP; 133 A; 101 C; 87 G; 110 T; 0 other; cacaaaaaca actctccatt atcctctttt ttcattttcc tacaaaatgg ggatattttt 60 tttccaaaca aaaataagtt aatgctttct ccatgcgcca tatgttacaa aaaaccagca 120 aaaagccgat ccaaaagcat gctacaaata ttagctgaac cgacaattcg ccaagttggc 180 atacaaagca cattgttatg taaataagga cacttaaggc ccattgggga tcggcacaga 240 gaggcgacac gatccaggag gttaaagtaa actgcaacta cgggctgttc ctaagctgct 300 tgctgatgtg acctaaaact atggttgctt caggtaccct tctccttcag caaccccacc 360 catagagggg gagctgctgg ttatagagct catcacctga gatggttgac agagaagttc 420 tgcctctggg g 431 // ID DY239159; SV 1; linear; mRNA; EST; PLN; 383 BP. XX AC DY239159; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H20.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-383 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 830e72f68389bc2cc70e89979c0184ad. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 20. XX FH Key Location/Qualifiers FH FT source 1..383 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 383 BP; 91 A; 84 C; 99 G; 109 T; 0 other; ctctctctaa aagactgaaa acaataaaaa ggaacagggg gggaagaaca ccaaccaagc 60 aggagttggt ttttgggaac tccgtgggct gtggaattgt ccccagaaat cccctctggg 120 tttgacgaga ttcatacaga tacatatatt taattttttc ccctttcgtt tgctcaaaaa 180 atcatttgta caggcaaaac tgcttgtttg tgctttgttt cttccgtgtt tgtttccttg 240 ggattttcct ctggtccgtt cttcttcacc tggggggtgg gaggagcaga gacaatcatg 300 ggaaagcagg gaccctgccg tctttgcgga gtcacaagta ctccgctgtg gcggaatggg 360 ccaccagata agccggtgct ctg 383 // ID DY239160; SV 1; linear; mRNA; EST; PLN; 448 BP. XX AC DY239160; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H21.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-448 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; d704e6a1fdee533ed0e6f4f127e3f208. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 21. XX FH Key Location/Qualifiers FH FT source 1..448 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 448 BP; 65 A; 171 C; 102 G; 110 T; 0 other; cttcattctc tcctccgtcg tcccgtcact cgtcagtcct cgttctcttc actcgatgaa 60 agggaggctg gtggagtgga agcgcttcta ctcctctttc gtgccacccc agcctcatcg 120 ccttccactg gactcatctg cttggcactc ctcgccccgt gcttggattc ttgcacttgg 180 tgcttccctt ctcctacctc acatcacccg cccagatcca atccacaaga gatttgagac 240 gctccggcct cccggcacaa accctttcta gggatcgcca ctcgccacac gccggccggg 300 gcgaaaaaag ctcttcttgg agcccttctg cttggcctct ccctctcttc tctcccggcg 360 tcattgtcgc cgctgcctgc gagcaacaac aattatggcc gccgcgacgt cgcgcgggcc 420 agcgtgggtc gccatctgtg cgctcgcc 448 // ID DY239161; SV 1; linear; mRNA; EST; PLN; 568 BP. XX AC DY239161; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H22.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-568 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 563894cda749f3b8562108d9b8426bf6. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 22. XX FH Key Location/Qualifiers FH FT source 1..568 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 568 BP; 115 A; 195 C; 148 G; 110 T; 0 other; cgatgcttga cggcacatga gtagtagtag tagggtcgtt ggcgtcgtcc tccgaagcag 60 gttctgcgtc acgacccagc acaccgcaac caacagcaca catgagctcc gactctgggc 120 taccagcagc agcaggcatc accggcgcca gcgcttcttc tccatccacc tgagcaagcc 180 gtgagctcag cagctccttt gccgacggtc tcgcctcacc agtaacaacc aaaggggtcg 240 caggaggtaa cagagcatcg ctcaggctca gccgctcacg cccgggtcca accgccaggg 300 gctgccctac gccggcctcc gaaacggact gcaccagcac ttgctccatg cggcacttga 360 acagcgaggt ggcctcgctc atggagcctt cttctccttc ttcatcaacg acgacgtgtt 420 cctcagcacc gccgtcttct tcttcttctt cagatagagg acgcagtact accgatcgcg 480 cattgcctgc atctgcggaa gctgaatcgt ccgcatcatc cgacccgctg ctcgacacgg 540 gcttttggac atgagaggaa tctgcggc 568 // ID DY239162; SV 1; linear; mRNA; EST; PLN; 410 BP. XX AC DY239162; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H23.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-410 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; f8d37fda0610348df5e24f54a1f79cc1. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 23. XX FH Key Location/Qualifiers FH FT source 1..410 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 410 BP; 125 A; 100 C; 110 G; 75 T; 0 other; gataaggagc cgagattctt cacattgaca ttaggtcacc ttcaaatcca attccaagag 60 ttgcaaatta cacatttcgg aaatcttttc acctgggtta cagtatacac taacaagttg 120 tcattgcgtg gcaaatcgaa cacaaaagaa cacgagcatg gtgctatgat acatcgacgt 180 gacagagaac gtaccccggt atcagatgac ccagaaccaa gccacaatgg aaccaatgac 240 aaggccaaca acgaggaaca gcacgatgac gatcccggca gtctccgagt ggtggatggg 300 aacagccttg ggcgcgagga tcatgaggac ggaggtcagg tacccgttag tgagccccag 360 gaggcacgtc agcacggtga cggggatctc ggtgcggaaa aagctcggcc 410 // ID DY239163; SV 1; linear; mRNA; EST; PLN; 594 BP. XX AC DY239163; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H23.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-594 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; ec7eff6d5fa0b16c4cc628ac85bc6712. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 23. XX FH Key Location/Qualifiers FH FT source 1..594 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 594 BP; 80 A; 225 C; 173 G; 116 T; 0 other; caaccatggc cgtcggcgac gtgaagacgg cggcgccgct gctgcccccg ccggcgtctg 60 ccctggagga cgagccctcc gcgggcccgc ccccagacga ccggctcggc atcggctacc 120 tcatcttctt caccctgggc gccggtttcc tgctcccctg gaacgcgttc atcaccgccg 180 tcgactattt cgatttcctc tacccggggg cccccgtcga ccgcgtgttc tccatatcct 240 acatggtctc cgccttactg cccctgctcg tcatcgtgct cttcttcccc aagtccagcg 300 cccccatccg catcaacacg gggctcacgc tcttcacgct cacgctcgtg ctcgtgcccg 360 caatggacct catctacgtc aaggggaggg ccgggctgta cggagcgttc gatgtgactg 420 tcgccgccac ggctctgtgc ggggtcgccg acgcgctcgt gcagggaggc gtcatcggct 480 tcgcggggga gctgcccgaa cggtatatgc aggccgtcgt cgccggaacc gccgcctcag 540 gagtgcttgt ctcggcactg aaagtctttc ccaaggcact gtacccacgg gacg 594 // ID DY239164; SV 1; linear; mRNA; EST; PLN; 213 BP. XX AC DY239164; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257H24.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-213 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 95d84d0bb146b091f9c55daa443aecf0. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: H column: 24. XX FH Key Location/Qualifiers FH FT source 1..213 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 213 BP; 70 A; 43 C; 51 G; 49 T; 0 other; aatcaccaaa tcgtgcatga tcagataaga gataaggcag acagataagt aggattacga 60 cagagagctc tattaatccc ttgatacgcg agccgagcaa accagtgggc gtttgaactg 120 tttttatgtg ccccgatcca atcaaatcgc gaaggaggaa ccgcgtatca agaggtgata 180 atcttaactt tgtattctcc agtgaagaaa gcg 213 // ID DY239165; SV 1; linear; mRNA; EST; PLN; 390 BP. XX AC DY239165; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I01.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-390 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 61fba9d1e839692026c8b6c62e6de3fb. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 01. XX FH Key Location/Qualifiers FH FT source 1..390 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 390 BP; 120 A; 77 C; 86 G; 107 T; 0 other; gtgggtctga ggcccattac aatagaggca ttacaataga gcatacagtt acgctaacag 60 catcaaatgc ctttgaaatc tctagcttca aaaggatgtg agggttcttt acttttatgt 120 agggtctgag caagctgctg cacaagtaaa aaattgttgt gaatattcct cctaacaaag 180 gcgctctggg tgttagacca gcttaggcag cagggtgcca agcgataagc aaaaatcttg 240 gttaccaact ttgcaaagct tgagttttaa gacttgagct ttacaagaag agtttacagc 300 ttggacttga tcagatccca tctgaggaaa aggatctcca gttttggcac ttgaattatc 360 accattgtcg aatagccacc ctaaaatcaa 390 // ID DY239166; SV 1; linear; mRNA; EST; PLN; 324 BP. XX AC DY239166; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I02.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-324 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 938f117a9f06feda7b1b67a9dd2a3e65. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 02. XX FH Key Location/Qualifiers FH FT source 1..324 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 324 BP; 98 A; 75 C; 60 G; 91 T; 0 other; gaaaaaacga ccatgcattg aggaaaatat caaagttcaa aatgatcatg caagttcgca 60 ctcgagaaag catctctaac agtctaacgt gcgacaaatg caaacgcgac gcaatactcc 120 aattcctctt ccatgtccca ccttcagatc gatttgcaga tatctgtttg gatattgtcc 180 aggtatgtga cgcacaagtt ccttgaacaa ttacaactga aatatgccgc ccagccatat 240 tcatccctgt ctttcttcac gtcgtgttac ttttggaatt gaatgctgaa agaaccagtg 300 tttgggtctt tgacagcttt gaaa 324 // ID DY239167; SV 1; linear; mRNA; EST; PLN; 344 BP. XX AC DY239167; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I02.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-344 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 7574cdd3a9d048ec75d49aaf3fbde894. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 02. XX FH Key Location/Qualifiers FH FT source 1..344 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 344 BP; 57 A; 127 C; 89 G; 71 T; 0 other; gactcctcgc gtccaccttt cggtctcctc ttctctgcga atcccgtaat cccatctctc 60 tcgcctccgc tgccgcctct ccgtctccag tttaccaccg ccgccgctgg atgtctacgc 120 agtagatcca tagaccctga tctcctcgag gtgattcccc tcgtcgccag aggtatcctc 180 gggcagcaat atccagtgga gacgcagccg ccacagccgc tacgttccct cctccaccgc 240 catcaccacg gataggtgga agcgctcgga gaggtagcgg ctcgggggaa agacatggtg 300 ctgatcgagc tggagcccga ggaggcgacg cgcccctctt tgcc 344 // ID DY239168; SV 1; linear; mRNA; EST; PLN; 547 BP. XX AC DY239168; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I03.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-547 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; c26451f7f09e91c2722b75240deb5471. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 03. XX FH Key Location/Qualifiers FH FT source 1..547 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 547 BP; 147 A; 112 C; 147 G; 141 T; 0 other; acgtagaaca gaactctgaa taagataatt gaattttgca caataaggtt tccttaagtg 60 tgacttcaaa agcccacacg gctcgcttat tatctcctag atttgtgtgg taacccctct 120 catatgtacg cgacctggca atatatccat ggtggtatac caatcttgta tttgtacaga 180 tgatgcttct gacaagagct cggtggtgca aagagctaat ggggttcagc agatgggttc 240 gtcagaagat ccagtgcacg tttcaggtga tccacagcaa cagatacgtc atcgaacgca 300 agagcaccaa ccgcaaatct ggctgccttg tgggcctcag caatcttctc aacttcaggc 360 tggtaactgc tatcatatct atactggcta gctggtggcg cagctggagg agtgacttga 420 ggtgaacagt cagcggttgg gtggtagtgc gttgggacag aagaatttga ggcaggagtg 480 tgtgatgtag ctgcgggatc atcactttca ggatagaatg aagactgatg cgtcagaaca 540 gaagctg 547 // ID DY239169; SV 1; linear; mRNA; EST; PLN; 608 BP. XX AC DY239169; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I03.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-608 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 2678ff7a24a3a8147eb756d0904b9827. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 03. XX FH Key Location/Qualifiers FH FT source 1..608 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 608 BP; 170 A; 165 C; 151 G; 122 T; 0 other; ccccgtcttc ttcaagctcc ggggatctca gccgccaaat ccgggccgtg ctcccgcttg 60 ggcatctgcg gcctacgatg gggagcgagt cagagcccgc caagggcctg ttgccctacc 120 tgcagcgcgc cgatgagctg caaaagcacg aacccctcgg ggcgtactac tgccggctct 180 acgcgatgga aaaggggctg aggatacccc aaaaggagcg caccaagacc acaaactcca 240 tcctcatctc cctcatgaac catctcgaga aggacaaaaa atcgttgact ttggggcccg 300 acgacaatct tcatttggag ggatttgcat taaacgtctt tgttaaagca gataaacagg 360 accgtgctgg acgagctgac ataaacactg ctaaaacttt ctatgctgca agtatattct 420 tcgagattct taaccagttt ggcgagcttc aacctgatat tgagcaaaaa cagaaatatg 480 ccatctggaa agctgctgaa ataagaaaag ctctcaaaga aggccggaag cctgaagctg 540 gaccgcctgg tggggataaa gatgaagcac ctgtcaatac caccaccatt tctcaagata 600 tggggcaa 608 // ID DY239170; SV 1; linear; mRNA; EST; PLN; 444 BP. XX AC DY239170; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I04.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-444 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 52810f857ea280f30c55b14a6d38aa11. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 04. XX FH Key Location/Qualifiers FH FT source 1..444 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 444 BP; 133 A; 107 C; 124 G; 80 T; 0 other; agccatgaag atgaagctga aatgacaaca gcataactaa caatgggtat tgattaattt 60 ccaattgtca tgtccaaacg aatagtgtct cccattctcc cctctttaca tatcgaaatt 120 aacagaaaag ggagaaatgg caaagcaaat aaaatgaatg gatcaatcga catgttcaga 180 cccaaattaa agtacttaaa tatatatata aacggaatcc aaccgaatac aggcaatggc 240 agccagcagc ctctccctcc gatcctcctt taattttact gcaggtagta agcgaagaag 300 gagagcacgg aggcgcccag cacggcgccg acggccggga acgcggccga ggaggcgctg 360 gtgggggcgg gcgccggggc ctcggcggcg aggaccacgg tggcggaggc ggcgggcggc 420 gaggacggcg caagcgacct tctt 444 // ID DY239171; SV 1; linear; mRNA; EST; PLN; 533 BP. XX AC DY239171; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I04.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-533 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 8dae490e384e9b932e275e15088d96e3. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 04. XX FH Key Location/Qualifiers FH FT source 1..533 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 533 BP; 100 A; 156 C; 142 G; 135 T; 0 other; caggcagggg cgcaaccaat aatagcaggg gtgatcatcc gttgatccat cttgctaata 60 agcctgcgtg cccttcgttc ttcctcgtct cgatcccgac gacgctccgt tcggctccgg 120 caaaccacat caagtcgcga tggagatgaa aaaggtcgcc tgcgccgtcc tcgccgccgc 180 cgcctccgcc accggggccc tcgccgccga ggcccgggcg cccgccccca ccagcgcctc 240 ctcggccgcg ttccggggcg tgggcgccgt gctgggcgcc tccgtgctct ccttcttcgc 300 ctactacctg cagtaaaatt aaaggaggat cggagggaga ggctgctggc tgccattgcc 360 tgtattcggg tggattccgt ttatatatat atttaagtac tttaatttgg gtctgaacgt 420 gtcgattgat ccattcattt tatttgcttt gccatttctc ccttttctgt taatttcgat 480 atgaaaagag gggagaatgg gagacactat tcgtttggac atgacaattg aaa 533 // ID DY239172; SV 1; linear; mRNA; EST; PLN; 578 BP. XX AC DY239172; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I05.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-578 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; efd41c1be9a3e347a81b232a1ab9ac13. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 05. XX FH Key Location/Qualifiers FH FT source 1..578 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 578 BP; 200 A; 163 C; 90 G; 125 T; 0 other; gagaacaaaa taaacaccac tgcatgcata ataattcaga gcaagtcagc aaaatctcca 60 gattgaaaaa aaaaaaagac aaatggctaa aaaccggatc aaacaaaaat aaaaacacgc 120 acacacacac acaggctaat gtataataca gcatcccttt ggttggtata catgctcacc 180 cgtatacagc ggacatctct ttccagcttc tgttggaaca tggcttgact tctaaaatat 240 gtgccgcaac aattactact acatctcatt tcaaaactcc aaaaggcctc accacgcgcg 300 tacgaaccaa aactagaaca tgaccaccac ccccgcaaaa accaaaaaac aaaacaaaaa 360 caaaaccttc acgaaatgtt tctgtatatc catccgcgct cctttcctct agctagccta 420 gcagcttcca cctctagcat gtcggtgttc gcaaaccacg ccgctgggtt agctacacaa 480 aaactaacct gtgtattaac ctgacaatca ccgaccgatt cacagccgtg tgcgccggga 540 ttgcctggct gaatgggtgt ttctttcttc taccaccc 578 // ID DY239173; SV 1; linear; mRNA; EST; PLN; 532 BP. XX AC DY239173; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I05.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-532 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 6eec042cf7450576d7388f47a8e22e2b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 05. XX FH Key Location/Qualifiers FH FT source 1..532 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 532 BP; 109 A; 168 C; 164 G; 91 T; 0 other; ccctccgcca ctccccaaga tgaaaacaag accattctcc tctccgaatt caataattcc 60 tccgccgcac cacgcttcct cctcctctgc tccgcctctt catccagaga gcgtcctccg 120 attgctgagg gatccctcct cgccgccgcc gcgggccgac cgtggggaga ggaggagagg 180 cgggagagat ggaggccgcc gaccacggaa agctctttgt cggcggtatc tcgggggaga 240 agagcgagga ccgcctccgc gagtacttcg gccgcttcgg agaggtcacc gaggccgtca 300 tcatgcggga ccgcagcacg gggcgtgccc gcgggttcgg ctttgtggtc ttcgccgatg 360 ctgctgtcgc cgagcgcgtc actacggaaa aacatatgat cgacggacga atggtggaag 420 caaagaaggg tgttcccagg gacgaccaca gtattgtgac caagagcaac gccagcagca 480 tagggtcacc tggaccggga cgcaccaaga agatctttgt tggaggcctg cc 532 // ID DY239174; SV 1; linear; mRNA; EST; PLN; 509 BP. XX AC DY239174; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I06.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-509 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 17e5f1c7012cafd133ba8355a97f7b77. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 06. XX FH Key Location/Qualifiers FH FT source 1..509 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 509 BP; 106 A; 147 C; 124 G; 132 T; 0 other; aaaaccatga tcaaaactta agctctgacc tttaagaaag ttctgcgtta tggacgataa 60 tccactttta ttcacgaatt gccatgaatt tgtttcctac gatcgagata atccatactt 120 attcacaaat tattcggtcc tgccagcctc agcagcgctt catgattttt ttatccctac 180 gatcgcccaa tcttctccac caggcgtctc tcgatctcca ttctcttcct catcaggatg 240 gtgtacttct ggaggagctc ccggtcctgc tccttgtcgc cggccgggac tgccagcttc 300 gagccaaggg aggtcttctc caggttgccc tggtacaggc acgagatggg gacgaagcta 360 ccttgcgcgt tgcacagcca cccgtgggca gctctcagcg ctgcgcccag cgatgccgaa 420 tcaggtctct ggactgtgaa gacagggcag ccgaagatct gcgcgactga cttgaggatg 480 ctttcgttgg aggatgcccc gccggttgc 509 // ID DY239175; SV 1; linear; mRNA; EST; PLN; 469 BP. XX AC DY239175; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I06.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-469 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; fb01bf6af02623e36a92baf28d0235a5. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 06. XX FH Key Location/Qualifiers FH FT source 1..469 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 469 BP; 103 A; 115 C; 124 G; 127 T; 0 other; cctccgcttc gctctcttgc tcttttagcc ccatctccca tcccacctgg aggcgaattt 60 cgtgctgggg aaaaagtcgg cggccgtggc cgggcgagac tccctcccgg agggttctct 120 cttcctcggg ttggacagct cctctcactc actgaaagct acggtgctca atagtgagct 180 caccatagta gcttctgaaa ttgttaattt tgactctgat ttgccacact acaaaactga 240 ggggggggtg tacagagacc cagcagatga taggcacttt ttttcaccac ccattatgtg 300 ggtggaagct ttggatgtgc tgcttgagaa gctaaaacca aagatcaact tcggtaaggt 360 agtggctgtt tctgggagtg ggcagcaaca tggcagtgtt tattggaaaa agggcagccg 420 tgctgtgtta tcctccttgg atgctagtaa aagcttgtta tcgcaacta 469 // ID DY239176; SV 1; linear; mRNA; EST; PLN; 615 BP. XX AC DY239176; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I07.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-615 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 1603add4be1fa4f5c1006cfdc86d60da. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 07. XX FH Key Location/Qualifiers FH FT source 1..615 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 615 BP; 160 A; 149 C; 156 G; 150 T; 0 other; gcgctaggct cggggatctt gtcgggtccc gtcccgtcct accacttcgc cggtgctgcg 60 tcgccgtcac tcgtcagtca ggactcagga tccccaagat ggtttcttca aacggagagg 120 ctgagcaaca gtggatagag aatatcaagt cgggaggaac agttccctgt ctagcacctg 180 aaaactgtcc taatgggtgg gctactcctc ccggagattc atttatggtc agaggcccaa 240 aatatcttac aaacaaggta aaaataccag gtggagagta tcttctgaag cctctcggct 300 ttgattggat caaaggacca gcaaaaatct gtgagatcct caaagacagt aaccatcgag 360 ttagaaaagc tatcgatgat gaggtttcac gtaataacca gccttttgtc tgggcattca 420 acctgcaact gcccaaggat aacttcagcg caatattcta ctttgtgtcg ctggaggcta 480 tacctgaagg ctcccttatg gaccagtttt tgaaaggaga tgatgccttc cgaaactcca 540 ggcttaagtt aacagcgaac attgtcaagg ggccctggat tgtccggacg gctgttggtg 600 aacaggccat ctgta 615 // ID DY239177; SV 1; linear; mRNA; EST; PLN; 509 BP. XX AC DY239177; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I08.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-509 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; f4fbd772cb47353ece0abeee769bc8a7. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 08. XX FH Key Location/Qualifiers FH FT source 1..509 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 509 BP; 106 A; 144 C; 128 G; 131 T; 0 other; cctctatctc tctctctctc tctctccctc tctacctgcc ccgttcctca ccagtctctt 60 ccgccggcgt cgacgagagc tgcgctaggt gatctcgagt gcttcctcgc cccccaggga 120 gacggcgctc aaatcgttcc tgaacagccc cgtcggcccc aagaccaccc atttcggggg 180 ccctgtcgcc aagtggggct tcgtcatcgc gggtttggtt gacatgaaca agcctcctga 240 aatgatatct ggcaatatga cagcagccgt gtgtgtgtat tctggactct ttatgaggtt 300 tgcgtggatg gtacagcccc gaaattattt gcttctggcg tgccgtgctt ccaatgaaag 360 cgtccagctc tatcagttat ctcgctgggc taggtctcgg gggtacctgg agaagaaaga 420 accagaggcc aaacagtaaa gagggtatcc aacactgcag tcatttgttc aataaagaaa 480 atatggtgtg gaaaacattc attctgttg 509 // ID DY239178; SV 1; linear; mRNA; EST; PLN; 498 BP. XX AC DY239178; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I09.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-498 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 76fdcc5ea9b36f135c3f97b2a973316a. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 09. XX FH Key Location/Qualifiers FH FT source 1..498 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 498 BP; 131 A; 102 C; 102 G; 163 T; 0 other; agtcaactcg agcatcgaac aatgaaaatt atcatttatt aaattacaac acgatgtagc 60 aggcaacttt tgtttcctgc tactaaacgt ctaatgcatg aatggtagtt catttcctac 120 aaggcctgtt gccagaaatg gcaacaaaaa ctatagtatc tatctcgccc aatgccgatc 180 tatgtaccga aatcatttgc agtgcaggcg tcttgaaatg aagaggctga attctgtacc 240 gagttttatt tagcttccaa tactatcttt cgatccacca tttggtgtag gctgttgatt 300 agttcttggg gtttcaatag tgcttgaagg aggagatttt tcttcatctt ctgttgcaga 360 aagggcaggt ggtgctccat gatgctgttg ggcatccaat tccattgctg cctctaatga 420 ggatttgtgt tcatttattt ttccacgtgt gataatcacc cggagatact ctcgcaaccc 480 agtcaatact aggttttc 498 // ID DY239179; SV 1; linear; mRNA; EST; PLN; 488 BP. XX AC DY239179; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I09.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-488 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 21536e476e94adc4b2651850b6373e71. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 09. XX FH Key Location/Qualifiers FH FT source 1..488 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 488 BP; 84 A; 154 C; 126 G; 124 T; 0 other; aacctccgtt gtcccatcct cggcgtcttc tccccgagcc cagcaccacc ctcctcgatc 60 ccaaagacca gaaagatttt gcctttcctc ttatcttctc ctcaaattca attcctcgaa 120 atctccctta ggggcatcga ttccttcgat cttctccgct accccctccc gtttcccgct 180 gcttattgag ttttggactc tgcgtgagtc tatcaactcg cccttgttaa gggattcatt 240 gtccaattgc ggtagctcgc gcggcaagat tcggtttttg agtcggtgcg cgacgggagg 300 ggtccagatt gctgagcccg gctcgacgtt ctggcctgag acctaattcg gtggcattgt 360 ggtgtggtga tggagcaggg gaggaatcaa cagcacccgc agcagttgtc gagtccgcct 420 gttttggccg ccgcgagcga gataaaggcc cgtgcggtgc cttcgcaaca gcccaagccg 480 gcggcccc 488 // ID DY239180; SV 1; linear; mRNA; EST; PLN; 427 BP. XX AC DY239180; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I10.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-427 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 90c13d24f877b2869194c18569f2eb2b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 10. XX FH Key Location/Qualifiers FH FT source 1..427 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 427 BP; 97 A; 144 C; 95 G; 91 T; 0 other; gcaacgaact ctgacatcac ggaaattttt ctatgtaatc cgattcaact actactaatc 60 cactacatca agaaacttat ataagtaaaa taaataaaaa aacattacct tcaccttcta 120 ctctctctaa acaaagctaa tggtatttac ggtcttcttc ggccgacaat acagctcagg 180 aacccatctt gccggcaggt ttgcatccag cggccgagat cagcgcggtc gtcttctcct 240 ggagctcgcg aaccacctcc accatggagg gccgcgtccc ggggccgtcg ccgatgcacc 300 gcagcgcaag cgccgccacg gtggccgcct cgtgcgtgtc gtactcgtct ccgagcctcc 360 ggtccactgc ctccgctact cttccttcgc cgagcgtagg acccaccgcg gcggcgagcc 420 ggcgccc 427 // ID DY239181; SV 1; linear; mRNA; EST; PLN; 517 BP. XX AC DY239181; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I10.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-517 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 29e09230e943e90cf57d59005cd92b30. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 10. XX FH Key Location/Qualifiers FH FT source 1..517 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 517 BP; 76 A; 198 C; 161 G; 82 T; 0 other; gccaaaaagg cgctcgccca ccgcttcacc aaatagagca acacagctca cctcatctca 60 tcctcggacc agcagccggc gtgcggagct gcggtgcggc gtcgtgcgtg cgccaaaaca 120 ggatgctgcc cgccgggtgc ggcctcctct cgtgcctccg cctcgacaag cggggcgacg 180 cgggccgaac cgacccccgc gtcgcggacg agcccgccgc gtctctcggc aagggcagcg 240 ggtcgtcggc caggcggttc gcgtgggccg agatcgagtc cgtcacggcg ggcttctcct 300 cccgcgtcat cggccagggc ggtttcagca ccgtctacct ggcctgcctc tcctcctccc 360 gcctcggcgc cgtcaaggtg cagcgcagca gcgagcgcct ccaccgcgcc ttccgccagg 420 agctccacgt gctcctctcg ctccgccacc cgcatatcgt ccgtgtgctc ggatactgcg 480 aagaacgaga ggaaggcgtg ttgctctttg agtacgc 517 // ID DY239182; SV 1; linear; mRNA; EST; PLN; 465 BP. XX AC DY239182; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I11.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-465 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 4fe1a83c74df004fface8ef8409b15e4. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 11. XX FH Key Location/Qualifiers FH FT source 1..465 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 465 BP; 105 A; 129 C; 131 G; 100 T; 0 other; gcaggggcaa aggattccat tagctttatt gttctgctgc ttcaaggcag catgcagctc 60 caggtcatcg aaaattcctt ttgtcgttga tttcgacata ccgtacgtgg ccagtcaata 120 ggtgtgtcgg acactcggtg ggtggcgcta gaccctagct agcacgtgta acaaaacgca 180 ctatacgaga ctagtcaagg tatatatatc tagctcacct aagaaaccgt cgtcgtctac 240 ttcatcttct tctaccaaga gctacaccta acagccgctc caggccaggc caggccaggc 300 cccagctttc caagcgaggc cggcgcagta gtccaggtca gctcacttgc tgctggttgc 360 catagaacgg gtaggcgccg taggctggag cagcgtacat gcctggatca gggaacgggg 420 ccgccgcagg gtaaccgtag ccgccgttgt agaacggggg cgccg 465 // ID DY239183; SV 1; linear; mRNA; EST; PLN; 584 BP. XX AC DY239183; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I11.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-584 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; fc1844c081ca1a106cb1ba5c53341562. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 11. XX FH Key Location/Qualifiers FH FT source 1..584 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 584 BP; 112 A; 175 C; 181 G; 116 T; 0 other; ctccactcgc gtccaaattc tctcctcacc acaccacctc tccctccgac gcacgggtgc 60 atcgcggtgc taaatagagt gaccatgcag gcggcggcgg tgacggtgaa tggcgcggga 120 gacgtgcaaa acccccagca gcacccgatg gggggggggg cgccgccgcc ggcggccatg 180 gtgccgcccc tttgggttgc cataccgatg ccgccgcacc cgatggcgcc gccccagttc 240 gcggcggctc atttcgtgcc gttccacgcc gtcgtgcagt cgccgcagct gggggcacct 300 gtgccagcgg tcgtcctggg atcgccggcg ccgcatcagg ccgggcagga ggagaacaag 360 accatctggg ttggtgatct ccatttctgg atggaagaga attatctgca caactgcttc 420 ggctacaccg gagaggttgt ggcaattaaa gttatccgga ataagcagac cggacagtct 480 gaaggatacg gatttgttga gttctacagc catgctgcag ctgaaagggt tcttgaaggg 540 ttttctggtc atattatgcc aaacactgac cagcccttta ggtt 584 // ID DY239184; SV 1; linear; mRNA; EST; PLN; 507 BP. XX AC DY239184; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I12.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-507 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 8122bcde94730b6084bbabd9faa0eadd. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 12. XX FH Key Location/Qualifiers FH FT source 1..507 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 507 BP; 155 A; 138 C; 111 G; 103 T; 0 other; gcttttgtag tgctgccagt aaggattcac tgcaataacg aagttgatcc atgtctgctt 60 gtacaacatg tcatatcagc ctttcacata aagcagcata ccacactaac tgtgaagcct 120 gtagcccatg tccctaaggt agacactaaa aaaaaacttg acctgcgaag ggtaagacag 180 cccccgggta ttgtattaag aagaagacct tctcacacag gtcaagaaaa cccccgaacc 240 cctgccccac ccatacacag cggcatcgaa gcccatgtga gaacgaccac gaccggggct 300 gaaccttaga cctgtgcttt ggcatgggac agacgagggg atttttttaa ccacagcctg 360 aaattcgctc ccacggggag tcaaacccag gacctgagga gtgctactca gaccaactaa 420 ccaactcagc tagaggccct ttcgcaccta aggtagacac tagacgagaa ggaaaatatg 480 cagcagctaa tctaatagtc ttgctac 507 // ID DY239185; SV 1; linear; mRNA; EST; PLN; 539 BP. XX AC DY239185; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I12.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-539 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; c4ca7d4c2dafdb14307f9d7ef02e4c93. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 12. XX FH Key Location/Qualifiers FH FT source 1..539 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 539 BP; 101 A; 206 C; 147 G; 85 T; 0 other; aatgacgaag ccacaaaaga aaagggagaa gccgccgccg ccgtcgtcga aaagcaagcc 60 atgaagttca aggccttctt taccgacgag ggcatctccc tcctcgacaa gcgattccta 120 ccggccatgg acaagggggg gcgcgtctgc cacgttttct tcacgcccac gcacgccgtg 180 ctcctccaca acctgctcgg cgccacggcc gctgggcccg acggcggggg cccgcagtgc 240 gtctctcatt tcgccaagga cctcctcttc cgtgagtaca acctgtcctc gcgaaacggg 300 aaccaaatcg ccttctccgt caaagtcgcg ctgcttcacc gcgcgctccg cagcgtgctc 360 gccgtccacg cgcacccgcc ggccgccggg gacgccgctg gtgcccccgc tatacaggag 420 aggctcgtca acaagctgcc cgcagggtca cgaaccgcca cgccattcct cactttcgaa 480 accaagggcg cccacgcggc cgtcgtgcag gatgtgccca tctcgcgccc tctctcacg 539 // ID DY239186; SV 1; linear; mRNA; EST; PLN; 536 BP. XX AC DY239186; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I13.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-536 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 9997bf7b4003d53589798f8868ebe634. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 13. XX FH Key Location/Qualifiers FH FT source 1..536 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 536 BP; 59 A; 211 C; 199 G; 67 T; 0 other; tgccatcctc tgcctgcccc ggttgcgcgg ggcggctgct ctagtgattt gagacggaga 60 tccgccaaag attgaggaga gggagggagg cgccgggccc ggggagcggc ggcggcgagg 120 gatggacggc cacgggggcg gggcgggggc cggcaagctg acgcggacgc cgtcctcgct 180 gctgcgctcg cccacggtgc gcaactgctc ctcgttccag gccgggggtg tcgacgaccc 240 ggagcccgac gacaagaagg cgcgggcgcc ccccaagccc ccgccccacc acttgcaccc 300 cggggggggc cccgcccacc cgcggctcgt cctcgcgctc ccgctcgcct tcctgctcgt 360 ggtgctcctc ctccgcgggg gcggagaggg ccaccacctc gccctccttt cggcgtccgc 420 ggccgtcgcg ctcgccgccg ccgcgggggc cgcgcgcctg ctccggggac gcctgcgccg 480 ctcgccaggc tccggctcgg tccagtggtt catcgggaac gaccacgaca acccgc 536 // ID DY239187; SV 1; linear; mRNA; EST; PLN; 473 BP. XX AC DY239187; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I14.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-473 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 758c812bccacd6a18826ad36803e8720. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 14. XX FH Key Location/Qualifiers FH FT source 1..473 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 473 BP; 59 A; 162 C; 193 G; 59 T; 0 other; cgctgcgccg ctgccacgag gtaaaagagg cgggacgagc gtcagtgcgg tgcgcgtgtg 60 gtcgggtagc gatccgagga gcgcgcagcg atggcagcgg cggccgtggc gactgtggcc 120 accccatccc ccgcctccgg gcggctgttc ccctccgccg gcccgtctct tctccggctc 180 ccagggccga ccaagcggct ccggcgcgcc ctgcggttcc ccgctgcggg ggatgaagcg 240 gacgtgctgc ctgccccggg ggccgagggg gaggccgtgg tgcccgggag gctggaggag 300 cacccggacg agcatctggg cggaacccat ctcgacatcg gagggctcgc cttccagggc 360 gacgtgggcg ggggcttcac cggcggcgga gcgggctccg gggcctccgg cggtggcggc 420 ggcaacaaga tgctggaccg gggcatcaac acggccatcg tgctcggggc cag 473 // ID DY239188; SV 1; linear; mRNA; EST; PLN; 628 BP. XX AC DY239188; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I15.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-628 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 0afe64c9778f20dad81a99ccae81705d. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 15. XX FH Key Location/Qualifiers FH FT source 1..628 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 628 BP; 135 A; 180 C; 173 G; 140 T; 0 other; ctgcgtggat gcagaagtgc tcgaaagcca gcttgaagtc agggatgtat ggcttgattt 60 tcatcttgag caccttcttg gcaatcaatg tgcccatgaa gagaagttgc tctgataatg 120 ggagcaccaa cggccccagc gtggtgatgt tggtcttaag tgcgtctccg gccactgcca 180 tgaggtcctt ggacagtgac acgccaatct tgccaatctc atcttcctcc tgtgtcacgc 240 agccgaagca cttgtcgttg gcgcccttgt gcgtgcgcac cgtgtgcacc agctcatact 300 tggacctccg cctgtcagac cgcctgttcg agagcaggat ggccgcgcca cccatccgga 360 acaggcaatt cgacacaagc atggaccggt tgttcccaaa gtaccaattc agtgtgatgt 420 tctccatgct gatcaccagg gcgtaagagt tggggtgcac ctgcagcaga tccttggcga 480 ggtcgatgga cagaagcccg gcgctgcagc ccatcccgcc gaggttgtag ctcacaatat 540 tccccctcag cttgtaatgg ttcaccacca tggctgacag cgacggcgtc gggttgaaca 600 ggctgcagat gaacactagg acaccaat 628 // ID DY239189; SV 1; linear; mRNA; EST; PLN; 582 BP. XX AC DY239189; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I15.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-582 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; f40b309a35428f14e1049106f1379621. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 15. XX FH Key Location/Qualifiers FH FT source 1..582 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 582 BP; 89 A; 218 C; 161 G; 114 T; 0 other; aagagacaaa agagggaagg cggcgggttg ccggcttaag ccttctctcc ctccgtcgtc 60 gcctcctcct tacgtccgct tcttcctcct gagagggaga cgtcagcgcc gcccagtgcc 120 cccgctccgc cggcgcagcc acggaggggg gtgccggatt tccagcagtc agtgcggctc 180 aagtatgtga agctggggta ccactacctc atatcccacg ggatgtacct gctgctgtcg 240 ccgctgatgg cgctcgtcgc cgtgcagctc tccaccgtct ccccgcatga cctcgccgac 300 ctgggggagc agctccgttt caacctcctc tccgtcgtcg cgtgctccac ggtgctcgtc 360 ttcctctcca ccgtttactt cctcacccgc ccgcgccccg tgtacctgct cgacttcgcg 420 tgctacaagc cggagtcgga gcgcaagtgc acgcgagaga ccttcgtgca ctgctccaag 480 ctcaccggat ccttcacgga agagaacctc gagttccagc gcaagatcct cgagcgctcc 540 ggcctcggag aggacacgta ccttcccccc gccgtgctcc gg 582 // ID DY239190; SV 1; linear; mRNA; EST; PLN; 476 BP. XX AC DY239190; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I16.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-476 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; e1db2cea841fdece879fe9ec244e2a4f. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 16. XX FH Key Location/Qualifiers FH FT source 1..476 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 476 BP; 122 A; 139 C; 84 G; 131 T; 0 other; ctagcccgca tagaatgtcg aatttggaat gccactacca agttcactcg tccacgatac 60 aaagccaaag caatccacag acagacaggt tctaccctca gttcaaagat gtggctaaac 120 catctcattc taataattcc tatacagcat caaaaaacga ccatgtgcct cactcctcac 180 ttcctcgtta aaataaaaac ggattggaaa caacttcatg ccctgtgcac cccttgttaa 240 ccatcctcat caaaaatcgt agtctttaag gtctttcttg gagtcggcca attccatctc 300 caccatacac tcctcttggc tgagaggcgg gagcccattc ttgtggcgtg tgtagttgtg 360 tttgtgccac ccactcttgc agtgctctct gtaaagcttg tcctccacga gtacatcaca 420 cctccttgca ccttctctgt tgcttgcctt gggcattgcc ctggctttca gaaaaa 476 // ID DY239191; SV 1; linear; mRNA; EST; PLN; 486 BP. XX AC DY239191; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I16.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-486 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 9ddafd7869ac9a7d7770bc8cde226f50. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 16. XX FH Key Location/Qualifiers FH FT source 1..486 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 486 BP; 98 A; 176 C; 130 G; 82 T; 0 other; gaaccgtcaa gactgtagcc cttaatcgaa accgtcccca acccttcttc ctccacgctc 60 tcgtctccgc cgcgctagga acttccaact cccaagccca gccgcctgcc tagggtaaag 120 gtcgcccctc cgccccctcg ctccgctcga gaccggcagg gttcgtcccc gccgttggat 180 atgtcgcgga cgctggtgca cccggggggg caaaagcccc tcaccaacgt gggtgtggtg 240 cggctgcgca agcacggcca gcgcttcgag atcgcgtgct tcccaaacaa ggtgctctcg 300 tggcgctccc gcgtcgagaa ggacatcgaa gaggtgctcc agtcccacac cgtctactcc 360 aacgtctcca agggcatcct cgccaactcc aaggaactcg ccaaggcctt cggcaccgaa 420 gaccttaccc aaatctgcat cgagattctg gagaaagggg aactccaggt ctccggcaag 480 gagagg 486 // ID DY239192; SV 1; linear; mRNA; EST; PLN; 578 BP. XX AC DY239192; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I17.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-578 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 81fe2020a3c957c4494f052582369126. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 17. XX FH Key Location/Qualifiers FH FT source 1..578 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 578 BP; 147 A; 174 C; 143 G; 114 T; 0 other; gatttgataa cctaacacca ttcattcatc tgatctgaaa gcatgtggta gaagctacgg 60 gagtactgct cgctatacac acaatgcaca taaaccaact aaaaaaactt acacctgtaa 120 atcacggcta tcaaggcact tacatgtctg taactatcct ccttccccaa accggaaaag 180 aatagtaaga aacactagta acaactggac aaaaaacaat caaagaatca gagaccgccg 240 tttcgcaatc tccatccgtc gttcttctgc ggtgatccgg tatttggcat tggcgtctca 300 gcccctccac tccgggcact ggtcggccgg ccgcgtgttc tgcgacctgg ccttgatcgg 360 gcacggcgcg tacaccggcg gcaccatgca gttgtacatc aggcagagcg acaccgtgga 420 agggagcgac gacgacggcg atatctcgat gcccgtcagg aagttgtgct gcaggtacag 480 cacctgcagg ccgccggagc ccaccagctc ctgcaccagc cgcgcgggga cctcgccgct 540 gaaccggttg ttgttcaggt acagctgccc gacgcccg 578 // ID DY239193; SV 1; linear; mRNA; EST; PLN; 608 BP. XX AC DY239193; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I17.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-608 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; add99a115364759cbef87e3cdeeb6e7b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 17. XX FH Key Location/Qualifiers FH FT source 1..608 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 608 BP; 130 A; 208 C; 181 G; 89 T; 0 other; ggggagtagc tcgcggcgga ccaaagacca aagaccaaag agcgagagcg agcaaagctc 60 acaaggcgaa gcggagttga ttgcgaccac caccaccacg tcttctccac ctccgtccga 120 tccgtcgtca ccaagccacc atcattggcg ccaccgcgcc gcccgcccgc gggagcccag 180 ctgcgcattc ttaagtgttt ctctcttggc ttctcggcaa agtgttgcgg ttgcgggggt 240 actggagtgc ggagagcagc gcggcggtac gtgtgcgttg ctgcgctgtg cttccacagt 300 gagctagggg agagcgaggg aggaagcacc ccccaccaca ccacctccat taaaggcgct 360 cacgcgccgc ccctccgcct ccacgagaaa gcgaggcggg agcgagtgag caggggcgta 420 cgtccgtcgt caaggccatc tctctcctcc tcacttcctc ccctcacggg gacagccacg 480 tacaaaggca gctcgccaac gcccagcacc tccctcgccg tgcgccttta aagacagcgc 540 gcgacaagct taaagctgca gaggaggtga cacagaggcc agagccgtcg cgcgcgcgcg 600 aaaccaaa 608 // ID DY239194; SV 1; linear; mRNA; EST; PLN; 489 BP. XX AC DY239194; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I18.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-489 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; ca6aa52c2f36d224a09e94e58a79787a. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 18. XX FH Key Location/Qualifiers FH FT source 1..489 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 489 BP; 144 A; 125 C; 123 G; 97 T; 0 other; gtgaaaatgt gaactcaacc ttgaagtcag cacacatcgc aaagacggac tgggaggcaa 60 cccaaaaaaa cgatttcgga aacagaaagc aaaaacgaag gaccttctgg cgctgctact 120 caagagtgag aactgagaac aaaagttctt cagatatacc atcacaggtt catcaatacg 180 actccgaggt ggtaggtagt ctaggggacc aactgagtca ctcagacaaa ccaatggaaa 240 actatacaaa attacggccc tggcatcaca cgttcttatt gtctttatta ttgtcaaagt 300 tgcattaggg gcaacgccat cacggccccg aaaactgaaa acatgacccg caccatccag 360 aagggcaccg ccttcttctg gttcttgtca cggcgcgcct ccgccagtgg ttgtgcccta 420 ttggccacgg tcggggcttg ctggacaaca ggggatggag caggggcagg attctcctga 480 ggcaccggc 489 // ID DY239195; SV 1; linear; mRNA; EST; PLN; 376 BP. XX AC DY239195; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I18.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-376 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 68f172dd21191d975187d6c6e77e2ff0. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 18. XX FH Key Location/Qualifiers FH FT source 1..376 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 376 BP; 60 A; 105 C; 130 G; 81 T; 0 other; cgatcccctg ccctcctcct tccagccgac tgagattcca ccaaatcggc gcccgtgcag 60 tccccgattc gattggcaag cacgattggc cgctcctgat cagctacggg acggggctgg 120 atttgatttg atcggatccc catttgggtg tccgggtccg cgcgttcggg ggggcgggcg 180 tgcggggggg gggattgctt tagggtttca gcgggtgcgg tcgctgtgta ggcggatagg 240 gttttgccat ggcggaaaaa ggctgcctga agcggctcca gagggagtac catgcgctct 300 gtaaggaacc gcccccgcag gtcgtggctc gcccactgcc caacgacata ttggagtggc 360 tctatgtact tgaagg 376 // ID DY239196; SV 1; linear; mRNA; EST; PLN; 491 BP. XX AC DY239196; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I19.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-491 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; b3ef897073189c5909b8850603551b4e. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 19. XX FH Key Location/Qualifiers FH FT source 1..491 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 491 BP; 145 A; 102 C; 111 G; 133 T; 0 other; gaatcattta acactaggaa atggaaaact tatttctgga cttggagtat ctcctgcaac 60 acatgggaat atgtcgttta ccctgatttc tgcagctgat gctcttctgg gctcatctgc 120 aagcaagtac agtgcattag actgtcagag gccaaaacta ttgaataaga ggaaagttcg 180 gggaaaaatt cttttgtggg gctattcatt caattacatt tctgggacag cctcaataaa 240 gaaagtgtct caaacggcca ggagtctcgg cgcagctggt tttgttgttg ctgtggaaaa 300 tagttaccca ggaacaaaat ttgaccctgt accagtcagc atccctggaa ttctcatcac 360 agatgtcagc aaaaccgagg atcttataga ctactacaat tcttccaccg taagagattg 420 gggtggaagg gctacagcct ttaaagcaac agccggtatt gcagatggtt tggcaccaac 480 actatacaat t 491 // ID DY239197; SV 1; linear; mRNA; EST; PLN; 482 BP. XX AC DY239197; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I21.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-482 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 7d21891bf91471531472037d64f1e6f8. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 21. XX FH Key Location/Qualifiers FH FT source 1..482 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 482 BP; 145 A; 105 C; 132 G; 100 T; 0 other; tccaggtaag gaaagtgttc gtcctcatgg cgccgccgca gccgaagtcg ggcctcttcg 60 tgggcatcaa caagggccat gtcgtcacca agcgcgagct gcctctgcgc ccgtcccacc 120 gcaggggaaa agcaacaaag agggtgtccg tggtcagggg cctgatcaga gaggttgctg 180 gctttgctcc ttatgaaaag cgtatcaccg agcttctgaa ggttggaaag gacaagcgcg 240 ctctgaagct tgccaagaaa aagcttggaa ctcacaagag ggcaaaaaaa aagagagagg 300 agaagatggg cgtcctcagg aagaagagat cgggtggtac gcacactgac aaaaaaaaat 360 aaagcatttc aagttcatga atgtggcagc cagagattat caactgtgtt ttttccctac 420 ctctagaaac tagacatgga aaaatatttt tgaccgaaac attgtttgtt aaaaatgtat 480 tg 482 // ID DY239198; SV 1; linear; mRNA; EST; PLN; 321 BP. XX AC DY239198; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I22.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-321 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 3ee25f1b3004402d7b3112b5c4ff0c00. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 22. XX FH Key Location/Qualifiers FH FT source 1..321 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 321 BP; 72 A; 107 C; 79 G; 63 T; 0 other; aaaagaacag tctattcatt caaataaaca ccagacagct agggtttcca tgcgaacaaa 60 cacgggccac gcccgtgcga cgccctgcta cctgcgctcg aacccgttca acacacacag 120 agaaagataa cgaattttag tactgcgaag accagtcggc ggcgctgccg acccttggga 180 tggtggtctc cttcctggcg cttgcgtcga ccgtgtacgt cgtgccgcac acctcgccct 240 tgctgtccac cctcggcacc ggcttcacct cgtggatggg gtgctcgtac ctcctccgcc 300 ctccggacgt cgtgaaaaaa a 321 // ID DY239199; SV 1; linear; mRNA; EST; PLN; 421 BP. XX AC DY239199; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I22.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-421 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; e0e415ea3a8a8b3020724c77d4d41d2e. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 22. XX FH Key Location/Qualifiers FH FT source 1..421 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 421 BP; 64 A; 122 C; 168 G; 67 T; 0 other; cgcttgtgtt gtcgcgattg gcaagctcgg cggctcgtgg cagcgggaac ttacgaggcg 60 aaggagagcg agggcgaccg cgcggatcgc atcgcagctc tgggcggagg tggggccttg 120 cgcggagatg ctagcggtgt tcgatcgggc gggggcgccg agcccggagg ggctgcggca 180 cccgggcgcc gcggggggcg gctgcgcggc ggccctcgcg gaccggttca gagaggcgcg 240 gcccgacgcg gtcaccgtca accttggggg gtccggcgcc ctggcgtact cctcgagcaa 300 ccagaacccc ctcctcccca gggtgtttgg cgcggtggac ggcgtattct gcatgttcca 360 gggaacaatt gcaaacgttg ctgtgctgaa acagcagtat gggttgagca aaggcgctaa 420 t 421 // ID DY239200; SV 1; linear; mRNA; EST; PLN; 418 BP. XX AC DY239200; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257I24.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-418 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; ae9dabbf15d7d3877209897e7178f979. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: I column: 24. XX FH Key Location/Qualifiers FH FT source 1..418 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 418 BP; 118 A; 127 C; 73 G; 100 T; 0 other; ccccactgca ctagtctgac cttctataat taaatcacaa ttcattggct cttacatcat 60 gtacctacaa tccatccatt ggctcttaca tcatgtacct acctacatcc atccagaaag 120 gaagaaacat gaaagtctaa agtacacgct gcatccagac atcaacaaga tcataattaa 180 gtcaggaacg ccaagaaccg ctctgcatgt catcactcag aaccatctgg taagaacctt 240 gctaacaaac aactgaagct gcagcatgta ccagctcata gcttaccctc tgctaagaag 300 ctgcgtctgc aggatctggt tgtcttccag caaccggtcg tactccagga gcagctcctc 360 cgactgcttc tgcagcgcat tgacatgggc ctccgctacc gatgccttcc gctcatgc 418 // ID DY239201; SV 1; linear; mRNA; EST; PLN; 285 BP. XX AC DY239201; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J01.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-285 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; c334f360b7d05fc7265b71dc3a2fcd74. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 01. XX FH Key Location/Qualifiers FH FT source 1..285 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 285 BP; 42 A; 105 C; 106 G; 32 T; 0 other; caaggagcgg aaggaggagc agcggcggaa gcagcgcgac gaggagggcc tccacctgct 60 gacgctgctg ctgcagtgcg ccgaggccgt gaacgcggac aacctggacg acgcgcacca 120 gacgctgctg gagatcgcgg agctagcgac gccgttcggc acctcgacgc agcgcgtggc 180 cgcctacttc gcggaggcca tgtcggcgcg gctcgtcagc tcctgcctgg gcctgtacgc 240 gccgctgccg ccgggctccc ccgccgcggc gcgcctccac ggccg 285 // ID DY239202; SV 1; linear; mRNA; EST; PLN; 543 BP. XX AC DY239202; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J03.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-543 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; e0f8a1d8a0d44a45713eaae75825c648. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 03. XX FH Key Location/Qualifiers FH FT source 1..543 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 543 BP; 161 A; 136 C; 108 G; 138 T; 0 other; aaacaaacaa catttcaagc aaaatgagat gtccaaattg cactgcttaa atggcaatga 60 tccctaaact ataccagccc tgaccacagg aactggtaac ctactgggat cataataata 120 cctacaatca aacctcagct acctaaagta ggcaatgcat ggaacagtca ggtttaatga 180 aggaccggaa atgtctcaac aatgaccacc atgggggtat atttagatgg atggcttggg 240 ctgtcctggt gcttcttctg ctacagcttc ggaaagctca agcgaaacag ggcagtggtc 300 acttccataa aatccatcca attcaatgcc acggccatgc atttcacatg aaactattct 360 gtcctttaac tgttccgaaa caaggaagta gtcgatcctc attctctttc ctcggtactt 420 gccaattgga tgaccggacc aagaaaagcc accgtctatg tccttttctt tgggcaggtg 480 cctgtaagca tctaccagct ttccttggaa aaatatgttg ccaaaacgcc gcctctctgc 540 tgg 543 // ID DY239203; SV 1; linear; mRNA; EST; PLN; 504 BP. XX AC DY239203; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J03.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-504 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; c78bcbf41a8b412119e4d529054b6087. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 03. XX FH Key Location/Qualifiers FH FT source 1..504 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 504 BP; 114 A; 142 C; 146 G; 102 T; 0 other; cgccgacgca gtccacaagc gatgaagcgc ttcttccagc cggtgcccaa ggacggctcc 60 ccgtccaaga agcggctagc gggtgctgct gaaccgggcg acggccccac atcacccggt 120 gccgccaccg gcgctggggg ggatgtagag gggaggccca ccgaggagcc gttcaagttc 180 ctgacgtgaa acgccaacag cctgctcctc cggatgaaga gcgaggggcc tgttttctcg 240 cagctcgtcg cccgccttga ccccgacgtc ttttgtgtcc agaaagtgcg gatgccggca 300 gctggttcca aaggggcacc taaaaacccc agtgaactaa aagatgacac aagcttgtca 360 cgggatgaaa agcaggtagt tctacgtgct ttgtcagctt cacctttcaa agactaccgt 420 gtctggtggt ctctttcaga ttcaaaatat gctgggacag ctatgtttat aaagaaaaag 480 tttgagccta agagggtgtc tttc 504 // ID DY239204; SV 1; linear; mRNA; EST; PLN; 514 BP. XX AC DY239204; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J04.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-514 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 37af03b64048d4c680d2a94c67de2677. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 04. XX FH Key Location/Qualifiers FH FT source 1..514 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 514 BP; 129 A; 161 C; 121 G; 103 T; 0 other; cacccatgca tgcatatcct tccattccaa atgttgctat ctccagtcta ccacttgcta 60 tctgaacatt caaatgttca aatattcaaa tgtctgaaca aataagtcgt cagcaattcc 120 tatcaagctg cgacggcgtc catcacaaca tggacagctc aaactaagct aaaatatatt 180 gatcacaaca tggccggacg atcaagtaca tgtaatgcgg agggagggag ggctctacag 240 acagacgact agtgtgggtg tggtagtaca acagcaacgt aaaagcacag gtttacttgc 300 actgccttgg gtggaacttc tcctccgaca gcaacgacgt gaccagccgc tgcagccgcg 360 cggctccacg gccaggcaca acaagcccgg tgtctccgac caccgagcaa gggtcgctcc 420 cgttctcccg cgtcccagcg caccagcccc cgggagtcgg cacgtccgcg ccgcggccca 480 ggatctcctc gtctatcctg tccagcaccc gggt 514 // ID DY239205; SV 1; linear; mRNA; EST; PLN; 469 BP. XX AC DY239205; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J04.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-469 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 9791ecea82d52df99f05607282fbbd38. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 04. XX FH Key Location/Qualifiers FH FT source 1..469 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 469 BP; 87 A; 148 C; 152 G; 82 T; 0 other; ccgttgtgtg caacgcggag gagttcaaga acacgacggt gaaccacgac ctgcactaca 60 tctcgtggga caaccctccg aagcagcacc cgcactattt gacggcggag gacctggacc 120 gcatggtccc cagcgacgcg ccgttcgccc gcaagttcca cgaggacgac ccggtgctgg 180 acaggataga cgaggagatc ctgggccgcg gcgcggacgt gccgactccc gggggctggt 240 gcgctgggac gcgggaaaac gggagcgacc cttgctcggg ggtcggagac accgggcttg 300 ttgtgcctgg ccgtggagcc gcgcggctgc agcggctggt cacgtctttg ctgtcggaag 360 agaagttcca cccagggcag tgcaagtaaa cctgtgcttc tacgttgctg ttgtactacc 420 acacccacac tagtcgtctg tctgtagagc cctccctccc tccgcatta 469 // ID DY239206; SV 1; linear; mRNA; EST; PLN; 536 BP. XX AC DY239206; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J05.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-536 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; e8c572f61bf5886b1f7800498979f1ba. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 05. XX FH Key Location/Qualifiers FH FT source 1..536 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 536 BP; 124 A; 170 C; 122 G; 120 T; 0 other; agagcaaact ataaccagat tccagaacaa tgaaaaacat ctggacccaa ctacccgatg 60 ccacctcatt cacaagcttc ttcccagtcg tcgacctcta catccggctg cagcttggtc 120 accgatggcc cagcattccc agtgtttgat actgtgctgt gcacatctgt gcccggagca 180 ggcttccgct cgttgcctga accagagctt gcagcagcgt ctgattccag aacattccat 240 cggtttgtag tccccatgac ttgagaggca gtgttgacgc ctctccatcc tggtgctgga 300 gcatgaccag caacaccaga ccagtctccg gagggcagat caggctcagc agaagcaggc 360 ttcttccatg gattgttcct agcggcgaac cccccatcct tctgctctcc aatccaaaca 420 ttgcctgaag atgcctgagc gctgcttggc ataaacatcg cagcgccctg gtaagcagac 480 ccataatcca gccgcctcag tgctgttgct gctctagccg gatcattgaa tacagc 536 // ID DY239207; SV 1; linear; mRNA; EST; PLN; 564 BP. XX AC DY239207; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J05.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-564 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 15bd46967363210d29ea22c1f503a87b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 05. XX FH Key Location/Qualifiers FH FT source 1..564 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 564 BP; 83 A; 255 C; 158 G; 68 T; 0 other; ctccgccgct gtatctattc cccccacccc caccgcactc cacccccttc accaacactc 60 ttccataccc caccgctaac ccaaacccga cggcgggggg cgccgcaacc ctagggttac 120 tcctcccctc ccgccgccgt atgcagccct ccaccgatcg ccgccgcgga gggggacccg 180 gggccaccgc ctcccgtgtg gggagagccc gccccgccgc ctcttccccc ggctccgccg 240 ggggccccgc cgccgccggc ccgattttgc ccctccctag ctcacccccg gaggcccgcc 300 cgccccgccg ccgctcgcgc cgttccaacc acggaaacaa ccaaaatcac cgccccgccg 360 cgcctcaaga gcaaaaagac aacgcaagac accgtcgccg cggtccgccg ccggagaggc 420 cggctcccgc gccgcctgcc cctgcccgtg agcacgcacc ggcgcctgcg gcggtggaga 480 ctcggggcga tggggcggtg ccgcatctgg tgcaggatat ccaggacaag ctcgcccgcg 540 gcgcggtgga gtgtatgatc tgct 564 // ID DY239208; SV 1; linear; mRNA; EST; PLN; 439 BP. XX AC DY239208; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J06.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-439 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 257f0bb259a85f34217d8882cfcc9536. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 06. XX FH Key Location/Qualifiers FH FT source 1..439 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 439 BP; 102 A; 124 C; 108 G; 105 T; 0 other; gtcttgctaa ccagttttat tgcttcagat gacagagcac aggaaacgta cacgtaaacc 60 cccattttct tattacttct aaatactggt aatttacaaa caaaggactg gctgatacat 120 aactacatat acaacggggg accaacccaa acaaacatga tctcatctgt tcccccttta 180 catgctgcac ccttcctgct ggcggaaatc gatcctcggc ccggccattg ctggtggatt 240 cagttccagc gcgagatcat caccggccct ggctcgggga gcgagtgaca tgtgatcgtt 300 gatgcggtcc tggagatgtc gagaaagttc ggctgacggt gcaccggagg cattcagggg 360 ttttacgcgg tgaccgaggt ctccatcgca acgcgttcgg ggtagagcac gtcctccgcc 420 ttctcggcct cctcccaca 439 // ID DY239209; SV 1; linear; mRNA; EST; PLN; 385 BP. XX AC DY239209; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J06.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-385 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; e21212d3bef1160c8e1e8c41edae32e0. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 06. XX FH Key Location/Qualifiers FH FT source 1..385 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 385 BP; 93 A; 118 C; 94 G; 80 T; 0 other; ggaagagttc tcttgaagcc tcaagactcg tctcgccgct gctccctcac ttctgccgct 60 gcgtgcctcc gttagctgtt gcattgcagc gcagccccgc gagccttgag ctgctatctc 120 gcccttcacc acagtcctca aacatggagt tcaaaaaccg ctgccacaag gtccaagagc 180 ccaagttcga gtgcctgctc ttcgacctcg aagacaccct gtatccgctg agcgccggga 240 tcgcaggcca tgtcaaaaaa aacattgaag attacatggt tgaaaagctg ggtattgacg 300 agaccaagat cgaaaacctg ggcaatctgc tgtacaagaa ctacggcacc acgatggctg 360 gactcagggc catcggctac agctt 385 // ID DY239210; SV 1; linear; mRNA; EST; PLN; 359 BP. XX AC DY239210; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J07.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-359 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 82cb055284f65ba7ecb5c1d4995dec81. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 07. XX FH Key Location/Qualifiers FH FT source 1..359 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 359 BP; 127 A; 81 C; 73 G; 78 T; 0 other; acggaattgg gggtgaaatg gaaccattca acagtttgca acatacaatt ccatccattt 60 cccaaggaga aaaaaggatg catcgtcaga gacgtcaggt actcaggtcg tcagctagct 120 gtccctgata aaatgtggca tgaacaacac gtttgtgaca gagcagcagt gtagctggca 180 actggcatgt acatactcgt acaacagagc attggtttca catcaaaaag aagaagaaga 240 aaccttccta aataaccgaa tggtattaca agtacaatat acaccggcga atacatctac 300 agcctaaaag aacctctcca tgacttcaga atccgacaag agttctttca acccgataa 359 // ID DY239211; SV 1; linear; mRNA; EST; PLN; 359 BP. XX AC DY239211; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J07.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-359 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 46c9bca1027b02932293ea7705335714. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 07. XX FH Key Location/Qualifiers FH FT source 1..359 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 359 BP; 79 A; 73 C; 79 G; 128 T; 0 other; ttatcgggtt gaaagacctc ttgtcggatt ctgaagtcat gaagaggttc ttttaggctg 60 aagatgtatt cgccggtgta tattgtactt gtaatacctt tcggttattt aggaaggttt 120 cttcttcttc tttttgatgt gaaaccaatg ctctgttgta cgagtatgta catgccattt 180 gccagctaca ctgctgctct gtcacaaacg tgttgttcat gccacatttt atcagggaca 240 gctagctgac gacctgagta cctgacgtct ctgacgatgc atcctttttt ctccttggga 300 aatggatgga attgtatgtt gcaaactgtt gaatggttcc atttcaccac caattccgt 359 // ID DY239212; SV 1; linear; mRNA; EST; PLN; 368 BP. XX AC DY239212; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J08.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-368 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; b208a8bf9c7fe0753c4c42a9a1944de5. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 08. XX FH Key Location/Qualifiers FH FT source 1..368 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 368 BP; 58 A; 127 C; 115 G; 68 T; 0 other; atgcgggaaa agacagcaca aacttcacat tactgctcat gggggggggg tcacttccct 60 ttcggtatcc attgtttttt tttttttgca cacgtcacgg ggctcacggc tagtggcagt 120 aggctgctgc tgttacggcc gatcgagccg gccaggaagg cgcggaggcg ccggcgtccc 180 tgaccctctt cctggcgagc cccagctgct gctgcgagcg cttgcagtaa gcgaaggcgc 240 cctggatcag cccctccgac tccgaccggg ggctgctgct ccgcttcagc gccggcccgc 300 cgccagcccc ggagctcctc ctcgccgaag acgacgacaa cagaggcttc cccggcgccc 360 cggcggga 368 // ID DY239213; SV 1; linear; mRNA; EST; PLN; 466 BP. XX AC DY239213; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J08.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-466 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 1655e799b56e57b77d2df09871b04cd8. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 08. XX FH Key Location/Qualifiers FH FT source 1..466 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 466 BP; 78 A; 166 C; 168 G; 54 T; 0 other; caacgacacg caccccgtgg cggcgaggct gcgcaccggg tgctcccgcc gcaggaaggc 60 ggggcggacg gtgcacgcga agctgctggc gtcgcgggcc ttcttccggt ccttgttcgc 120 caggactacc ccgtgctccg acgagcaatg ccgcggcgcc gaggtccggg ccagcaccag 180 agcggcgccc ccccccggag agacaaagag cgactgctgc aaggcggcgt ttgcccatat 240 caataacggc tacggctacg gctaccacag cggcggggcg gcgccgacca ccctgcggag 300 cagcatcgag caggaaaagc tgatggacga ggaggagctc gccgccgccg cccggcagcg 360 caagtccttc tccggcgtga tcaagtgggg gcacgcggca gcggcgcctc ccgccgcggc 420 gccggagaag cctctgtcgt cgtcgtcctc ggagaggagg agctcc 466 // ID DY239214; SV 1; linear; mRNA; EST; PLN; 523 BP. XX AC DY239214; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J09.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-523 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 73c65ffdf4cd1677d87fe30b2fccce89. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 09. XX FH Key Location/Qualifiers FH FT source 1..523 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 523 BP; 151 A; 109 C; 129 G; 134 T; 0 other; aacatcaaca ctagcatagt tataacagaa agcacaagtc atctcattat cgacggcaat 60 ggtaggttga tgttggacta gcattcgatc cttagatgga tcagaatgca cacgggcgac 120 aaggaatgga agaacatacg tccccacaga cagtttcatg gggcaacacc acaatcgtct 180 gcatttaagc agtaatgccg aatatggagt ttcgacttca tttcattttc atcccatggc 240 atatttctgg gtccagctgc gggctgttgc ctcgtactta ggccgatccg tcttgtacat 300 gtgagcaatc tcagggacaa gaggatcgtc tgggttggga tcagtaagca gcgagcagat 360 agaaagcaaa accttagaga ttgtcaaagc agggctccac tgctccttaa gaatgtcgag 420 acatatgctt ccattactat tgatgtttgg atggaagacc ttcgtcttaa aaaatacctt 480 tggaggcttg aaagggtagt ccggggggaa atgaatgttc act 523 // ID DY239215; SV 1; linear; mRNA; EST; PLN; 512 BP. XX AC DY239215; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J09.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-512 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 27fbf93ed6fec30b75cc1c3fd1f74c40. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 09. XX FH Key Location/Qualifiers FH FT source 1..512 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 512 BP; 126 A; 150 C; 111 G; 125 T; 0 other; ccaccattcc ttccccaatt ctgcaggctg ctcgtcgccc tccctccacg ccggaggcgc 60 cccgatccaa atccaggggc gtaacatggc atcaaaacgt atcctcaggg aattaaagga 120 cctgcaaaag gaccctccaa cgtcatgcag tgcaggtcct ggtggagagg atatgttcca 180 gtggcaggca accatcatgg gtcctcctga tagtccatat gctggaggtg ttttcttagt 240 gaacattctt ttccccccgg actacccttt caagcctcca aaggtatctt ttaagacaaa 300 ggtcttccat ccaaacatca atagtaatgg aagcatatgt ctcgacattc ttaaggagca 360 gtggagccct gctttgacaa tctctaaggt tttgctttct atctgctcgc tgcttactga 420 tcccaaccca gaagatcctc ttgtccctga gattgctcac atgtacaaga cggatcggcc 480 taaataagag gcaacagccc gcagctggac cc 512 // ID DY239216; SV 1; linear; mRNA; EST; PLN; 620 BP. XX AC DY239216; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J10.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-620 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 3bef726b8e0da165aca21827b78c1f8f. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 10. XX FH Key Location/Qualifiers FH FT source 1..620 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 620 BP; 98 A; 197 C; 215 G; 110 T; 0 other; gtcggcgact tgggccgcgt cagctcgatg gccagcggca tcagcgtgcc caggtccgtc 60 aggaagaaga tggtgcgcga ggcgtagagc gtcgcgtcct gcagctcccg caccttgtgc 120 acgtagggca ggaacacgtc gtggtagtcc aggatgaaca gccgcttcgc cgccagcgcc 180 tgctccacgg tcagcgcgcc gttcatctgc ttctccagga tctccttggt gatggcggac 240 tccgctggcc cgtacacctc cgggtccagc ttgctcttga tggggaactc agtcagcagc 300 tggatgcaca gcgggttgag ccccgcgatc gtctgcctcg cgaactcctc gtccttgaac 360 cacgagaacc ggtccctctc tatcatctcc ggcacctcga agcggaggac gtggtcggtg 420 gtgtcctcca ccagcttgac catgcgcggg acgacggtgc ggatggcgtc gaacccggtc 480 tgcgccggca gcgggatgcc gtcgctgaag aggtcgtcga tggcggggaa gtgcgggaag 540 cgcagatcct tcttgttgat gagcagcggg gcggagcgcc ggcagcagcg cgtgcaggcc 600 ggaacgcagc gtggtgcccc 620 // ID DY239217; SV 1; linear; mRNA; EST; PLN; 455 BP. XX AC DY239217; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J10.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-455 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 76ffe95b1bf2cfddc7c6288e58bd82a2. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 10. XX FH Key Location/Qualifiers FH FT source 1..455 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 455 BP; 79 A; 160 C; 147 G; 69 T; 0 other; aaacagctag ttataggctg tagctagcag ctagcttcag tcacaggccg gacggtcggc 60 tcacacacag ctagccgccg ccgccgctag cttacccacc agtagtccga tctgatctac 120 aggcaagggg caactagcta gctagccgcg cgccggcgcc atgatgaacc tgaacctgaa 180 gcaccctcgg gtgctgcccg cgcaccacag caatgtcgtc ggctcgcgcc tgtcgtcgtc 240 gtccccctcg gcagccgccg ccagcaggaa gaccggcggc ggcgtgtcct cccggtccgg 300 ctcccggcgg cacgtgcggc tgccgaggat cagctgcagc gccaccgaag aggtcagcgg 360 cgccgtgtcg tccgtcaccg tggagaggat gctcacggag acggcgtcgg tggaggcgtc 420 cccggccatc gggcagatgt atttccagcg cgccg 455 // ID DY239218; SV 1; linear; mRNA; EST; PLN; 511 BP. XX AC DY239218; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J11.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-511 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; ab3728da73c03840a352994f47e981c2. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 11. XX FH Key Location/Qualifiers FH FT source 1..511 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 511 BP; 101 A; 147 C; 145 G; 118 T; 0 other; tcacagatcc agccaggtat caagtcatcc ttccgccgcc gcgtactgcg cctatcttgc 60 ttctccggag gacgggcgta cccatcgtcc tcgatcatcc cccggactgg atcggaacgg 120 agatccaccc ggacctgcgg agatcccttc cttttctcgg atagtatagt gagctaccca 180 atgaagaggt aaattcgtcg ggcttgtatc aagttagtag ttcatggtgc ctggatgagc 240 agtagagatg ctactggccg tggaaggagg agggttcttc tcgtcttcat cttcggggta 300 cagccatggc cttgctctct tgctactcgg acggaaagct gaggaaaagc ccgtcaaggc 360 ttcgccgtga aaccagtacc ggctagtcga ccgggaggcc gagcaggtgt accacctgcc 420 ctccgccaag gatcaggcgc ctgggaaatg tgctcccttt gtctgtttcg gatgcacggc 480 taatggcctc gaggtggcgt ctccttccaa a 511 // ID DY239219; SV 1; linear; mRNA; EST; PLN; 478 BP. XX AC DY239219; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J12.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-478 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; dda9d80ad4672b923d65bb53726fcf0d. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 12. XX FH Key Location/Qualifiers FH FT source 1..478 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 478 BP; 151 A; 125 C; 103 G; 99 T; 0 other; gtataaaatt taaggcaaag ccaaactgca ttgcttaaca gacacagggt tgttgtcact 60 acccaattac attaagataa aatgtaacca actcaaaagg agaaacaaaa ggatatggac 120 taggtaccag tctatgccac ttgtcctctg ctgggacata caacaccaat atcaccactg 180 gcacggagga tatgccaatt cctaacccct caatcatagc tcgaattcat catccaactt 240 tagcgcctca gctgcagcct cctcttcctc atcatcaatg gcaaagtatg ggttatgcac 300 ctcaggccgg aacttgtacc cagtaaagac atagaacgcg agcgtcgcga gctccccggc 360 cacgacgcta gtccacaggt accggtatga ggtgatggtc tgtagcgcat acaccacaac 420 acgcgtgaag taaatgtagc aaaagacgac cacatagtac tggcggaaga gggtgagc 478 // ID DY239220; SV 1; linear; mRNA; EST; PLN; 502 BP. XX AC DY239220; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J12.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-502 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 583aba577ddd971eb2fbbf0c75965675. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 12. XX FH Key Location/Qualifiers FH FT source 1..502 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 502 BP; 61 A; 218 C; 134 G; 89 T; 0 other; ccacgcgccg ctcgcccgcc ctctccaaat ccaaaatcca acccaccggc caccgcacca 60 ccgccgctta tcgtccccac ccgcagcccc ccgcccccga tcgggggggc acccataagg 120 gccgcctcgt cgtcaccgcg cgccgggggg ctccgcgggc cctccgtgac cgtcctcctc 180 tttctcgtcg ccgcgatggt gtcggttccc cccgcggcgg ccgatatccg ggagaccgcg 240 atccgggccg acccgcggag catcatcccg ctggaagagt tcggtttctc ccactcgggc 300 gtgctggagc tcaacgtctc cggcatcgct ttcgatccgc aggcctccgc ggagctggac 360 ctctcccagc tcggtttctt cctctccacg ctcgacgcgt gggttcacgt cctccgccag 420 cttcaggacc tagacgtcac ctgcgcgctc cagtcggagc tcgtcaagct cgccttctcc 480 tttgagcgcc tccgcccgcc ct 502 // ID DY239221; SV 1; linear; mRNA; EST; PLN; 517 BP. XX AC DY239221; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J13.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-517 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 5f0556f6bd1ad185c3444a9f07617680. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 13. XX FH Key Location/Qualifiers FH FT source 1..517 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 517 BP; 117 A; 133 C; 175 G; 92 T; 0 other; gacaagcaaa tgtaaataca gcagcaaaca attaatgcat aatccagcag atcaaatcca 60 tctcatgaaa tgaaaataat ggtaagctag acgaggatga gcttgacgta gttgtcgaag 120 acgaagtcgg cgaggatctt gtacgccttc tccgttgggt ggtagctgtc ccagaagagg 180 tagtcgccga cgtcttggca gacggcggag gtgacgccgt tgcagaggac ggagacctcg 240 aggaggcccg tgccgcagca tcctctggtg gactgcgtga ggccgtagct gcgcgggtgc 300 atcatcatgt cgtagaggaa gccgtagatg tccatgaaga cgagcagcgt gtcggggtac 360 ttggcccgca gcgccgccag ctgctgcacc atcccggcgt tgtatgcgac ggcgatctcg 420 ttgtggccct gcgagcagcc ccggtcgaga ccgccggaca tggtgcgctg cgacgggacg 480 cagccgatgg gcgggatgcc gatgaaggcg acgcggc 517 // ID DY239222; SV 1; linear; mRNA; EST; PLN; 554 BP. XX AC DY239222; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J13.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-554 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; df6332a4640c46baf0f0d4c7aa2eec18. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 13. XX FH Key Location/Qualifiers FH FT source 1..554 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 554 BP; 117 A; 197 C; 129 G; 111 T; 0 other; gccagctagc catatatacg gcggcattct tcttctcttc ttcttcccca ataaaatatc 60 gagctgcaag ctgctctgct tcttttctag gaggggcatg agggtcttgt tgggggtcgt 120 gctcatctcg ttcgcccccc ttattaccac aaatgcagca gcgggtgtca acgccacgag 180 atctgctggt aggcagcaaa agcaaaagca aaagccgctg gcgcctgccc tcatcgtgtt 240 cggagactcc atcggggacc ccggaaacaa caacgacatc cacaccatca tcaaggccga 300 cttcccgccc tacggcaccg acttccaaaa ccacagagcc acggaaaggt tctgcaacgg 360 caggatacca accgacttca tcgcctcccg cctcggtatc aaggagcttc tgccgcccta 420 cctcacctca gagccgctcg acaagcaaga cctcgtcacc ggcgtcagct tcgcgtccgg 480 cggcacgggc ttcgacccgc tcacgccgca tctcgcctcc gtgatctcgc tcccggacca 540 tctcaccgtg ttcc 554 // ID DY239223; SV 1; linear; mRNA; EST; PLN; 473 BP. XX AC DY239223; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J14.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-473 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 3f2f5fe18af14b4ba01667549367d2f1. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 14. XX FH Key Location/Qualifiers FH FT source 1..473 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 473 BP; 136 A; 105 C; 112 G; 120 T; 0 other; tgcatcgaca ccacggggcg ggaagatcac agcatgaaca atgctgccta cccatcaaaa 60 gtgaccagca agacaagttt gtcgtcagtc ccttccacaa ttaagagcaa ctcaagtcgt 120 tcaacactga ctcttccatc aatgagagat caaaatgagc ttcctactcc tcggacagag 180 ggagaaatat tgtcatcttc tattttgaag gctttcacgt tcagtgatct aaaaaatgca 240 accaaaaatt tccgaccgga cagccttctt ggggaaggag ggtttgggcg tgtctacaaa 300 ggttggattg atgagcacac gcttgctcct tcaagacctg ggagtggtat ggttgttgct 360 gtgaagaatc ttaaaccgga aggttttcaa ggacacaagg aatgggtgac agaggttgat 420 taccttggcc aacttcacca caagaatctt gttaagctca ttggttattg ctc 473 // ID DY239224; SV 1; linear; mRNA; EST; PLN; 453 BP. XX AC DY239224; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J16.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-453 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 5ce8219b8a62ca969e4c9ce12d227f33. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 16. XX FH Key Location/Qualifiers FH FT source 1..453 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 453 BP; 105 A; 152 C; 122 G; 74 T; 0 other; aatcaagcaa gcaagcaagc aacgagtaca tacatactag gcagccaggc agccatggcg 60 cccaccgtga tgatggcctc gtcggccacc gccgtcgctc ctttccaggg gctcaagtcc 120 accgccagcc tccccgtcgc ccgccgctcc tccaaaagcc tcggaaacgt cagcaacggc 180 gaaaggatcc ggtgcatgca ggtgtggccg gcctacggaa acaaaaattt cgagacgctg 240 tcatacctgc cgccgctgtc gacggaagac ctgctgaagc aggtggacta cctgctgcgc 300 aacggctgga taccctgcct cgagttcaac aaggtcggtt tcgtgtaccg cgagaactcc 360 acctccccgt gctactaaga cggccgctac tgaaccatgt gaaagctgcc catgttcggc 420 tgcaacgacg ccacccaggt gtacaaggag ctg 453 // ID DY239225; SV 1; linear; mRNA; EST; PLN; 497 BP. XX AC DY239225; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J17.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-497 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; a814fc5b2df454e166cbadc259bca09b. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 17. XX FH Key Location/Qualifiers FH FT source 1..497 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 497 BP; 159 A; 136 C; 116 G; 86 T; 0 other; gaccaacatc tcaatctaca tcaagccacg gctactgatt ttattggcgt ttatgtactc 60 gaacgaacca aatgctgaca tcgaactaca acaaatgaaa taaacaaaac tgtccaccaa 120 catggcaaca tgcccaaccg atcaactcaa gcacacataa aaacatcacc tactcgtgac 180 acaggatcac ggatcaccat aaagggaaca tttgcacggg cacgaacggg aacggagctg 240 gaccgcacag gggaaacact gaggcaatgg tgcctacagg acttgcagca caccggccca 300 aacagggtaa atgaaaagca ggaggatgcc gatggagttg gacacaccat tgcccttcct 360 gaaggcgttc ctgaatccac ctgacgcctt gatgtgttct tggagcaggt agcatccgat 420 cacaaggcat atgaagacac caacgaggtc tcccctgaag ctgtccgcga acaggctagg 480 ggcgaccact gtgagca 497 // ID DY239226; SV 1; linear; mRNA; EST; PLN; 407 BP. XX AC DY239226; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J17.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-407 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 37aae3e169bcc480569a7b7e64d59ab1. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 17. XX FH Key Location/Qualifiers FH FT source 1..407 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 407 BP; 76 A; 115 C; 140 G; 76 T; 0 other; gacgaagcac agatccatcg gagtgacagc gagcgagagc aaaaaagaca aaaagattgt 60 ccgcgcgtca acgactcccg gcgaagacga tggggaaggg gttcgcatcc tacctggcga 120 tgaagacggg gccggagggc ggagacgcgg cggcggcgca gcgggctctg atcgacgcgg 180 acctgcggga gctcggcgtc cccgcgcgaa agctcgccaa ccacgcattc gtcctcgggg 240 gcggggtggg gttcggcccc tccttcctca aatgggtggc cttcctcgcc gcagtgtgag 300 tgtttccgct cccccaggcc cgtttctcta tggattcgtg tgagaagggg gatccgtgat 360 cccacttttg ggcggggtct ttgatcttat gattcgcgaa tcgttta 407 // ID DY239227; SV 1; linear; mRNA; EST; PLN; 380 BP. XX AC DY239227; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J18.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-380 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; dfc4e1f18c74aa30b706704f1f027f8c. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 18. XX FH Key Location/Qualifiers FH FT source 1..380 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 380 BP; 124 A; 96 C; 69 G; 91 T; 0 other; ggaaaaatta ctgtaacaac taacatgatg gcagaactac cactgaatca ccttggagtg 60 tgccttttga cattacaatt acacttttaa tgttgacggg acagcagcta gagaatgcat 120 cacattcaca tagagaccca cattccacaa cacgagtaca tggctgaagg acgtactatt 180 actattacca agcagtggct gcttatctac acacacccaa acccaggaca gaagttcagc 240 gtgaacccca aatacacaaa agcctcattg ttgcttgatt gctccaaatc ccaaccttcg 300 caaaatcaag ctccaaatcc atttgacagc tggagtgcgt gcccataaaa cggtatgttc 360 attctgcata gtttacaggg 380 // ID DY239228; SV 1; linear; mRNA; EST; PLN; 487 BP. XX AC DY239228; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J18.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-487 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; ecb7847cdf3a512491423eb3ec125ee4. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 18. XX FH Key Location/Qualifiers FH FT source 1..487 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 487 BP; 77 A; 187 C; 155 G; 68 T; 0 other; gcgcaatcat acttcctacc ctccccacct cttccacggt tcccccccgt ccctctcctc 60 tcaagaagca cccgcaccat acatcttccg gcgcccgacg cggagggaga gccgctgctg 120 ctgctgccgc cggaggagga ggggcaccag ggccagcggg tgcgggagga ggaggagcag 180 cagcgggccg ccggccgctt ggggctgtgc cacatgccgc tctggggggc cgccgcgtcc 240 gcggcgccgg cagccgacgg agaccccgcc cgcgcgccgt cgggatccgg cgggggcgtc 300 aaggtgatcc ggtcgctgct ccccacccgg cgccgcctcc gcctagatcc gcccgccaag 360 ctctacttcc catatgaccc cggcaagcaa gtccggagcg cggtcaggat caagaacatc 420 agcaagtccc gtgtggcctt caagttccaa acgactgccc caaagagttg cttcgtgagg 480 cctccgg 487 // ID DY239229; SV 1; linear; mRNA; EST; PLN; 486 BP. XX AC DY239229; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J19.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-486 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 181b6bc7cf7747cddf150ad8274494e3. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 19. XX FH Key Location/Qualifiers FH FT source 1..486 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 486 BP; 96 A; 159 C; 129 G; 102 T; 0 other; ggctgcacac gtcgtctcct cttctccact cgaacactcg caccctgcgt ccgcctgccg 60 cctaccccag gcggccccct cccatcgcca tgcctccgtc cgccgccgcc accgccgccg 120 ctttcctctt ccgggccgtc gagatcccct gccgcgtgtt ccctcaacgg ggggagagga 180 gcctcgccgt ccgtgccgtc ccctcccctc cagccttcgc ggtcaagccc gccccgctgc 240 cctccaagag tggaaagtgg gagtggaaat tcgaaaacaa gccggtaaac atatactatg 300 aggaacatga gcaggaggtt gctgaaaata tgaaaaacat tctcatgatc cctacgatct 360 ctgatgtgag cactgtggaa gagtggaggg tggttgccaa agatattgtt gggcggaaag 420 gagaacttgg ctaccgtgtt acaattgtcg attggcctgg tcttggttat tcagataggc 480 catcac 486 // ID DY239230; SV 1; linear; mRNA; EST; PLN; 491 BP. XX AC DY239230; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J20.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-491 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 782d6f727f50f1fba2d7a11baf120c70. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 20. XX FH Key Location/Qualifiers FH FT source 1..491 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 491 BP; 73 A; 179 C; 156 G; 83 T; 0 other; ccgcgctcca catcgccgcc gcgaatggcc gcctcgaggt gctgtcaatg gtcttggatc 60 acggggtgcc gccggacgtg gtgaatcggc acaagcagac cccgctgatg ctcgcggcga 120 tgcacggcaa gaccgactgc gtgctgaatc tcctgcgggc cggccccaat atcctcatgt 180 tcgactccca gcacgggggg acctgcctgc accacgcgtc ctatttcggc cacgtcgggt 240 gcctgcgggc catcctcacg gcggcgcgga ccacgccggt ggccaactca tggggtttcg 300 ctcggttcct caacgtgaga gaagaccacg gcgccacgcc gctccacctc gccgccaggc 360 agggccgccc cggctgcgtg caggcgctgc tggagaacgg tgccatcgtc tccgctttga 420 ccggctcata cgggtttcct ggtagcacgt cgctgcacct cgccgctcgc agcgggagcc 480 tgaattgcac c 491 // ID DY239231; SV 1; linear; mRNA; EST; PLN; 584 BP. XX AC DY239231; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J21.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-584 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 2e095853e68faac5caccfeb25f88a1e5. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 21. XX FH Key Location/Qualifiers FH FT source 1..584 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 584 BP; 117 A; 188 C; 145 G; 134 T; 0 other; ttcgtcccgc gcgccgtcct catggacctc gagcccggga ccatggactc cctgcgctcc 60 ggacccttcg gccagatctt ccgcccagac aacttcgtct tcggccagtc cggcgccgga 120 aacaactggg ccaaggggca ctacaccgag ggcgccgagc tcatcgattc cgtcctcgac 180 gtcgtccgca aggaggccga gaactgcgac tgcctccagg gttttcaagt ttgccattcc 240 ttgggaggag gcactggatc tggcatgggc acccttctca tttccaagat cagggaggag 300 taccctgaca gaatgatgtt gaccttctct gtattcccat caccaaaggt ctcagacact 360 gttgtggagc cttacaatgc tacactttct gttcaccaac tcgttgagaa cgctgatcaa 420 tgtatggtgc ttgacaacga agctctgtat gacatatgct ttcgcacgct aaagctcgcc 480 acccctacct ttggcgacct gaaccatctc atctctgcca ccatgagtgg cgtgacatgc 540 tgcctgaggt tccctggtca gctcaactct gacctgcgca agct 584 // ID DY239232; SV 1; linear; mRNA; EST; PLN; 460 BP. XX AC DY239232; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J21.r ZM_BFb Zea mays cDNA 5', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-460 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; 2840fd1d53e3e0c91b3903ce5314a633. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 21. XX FH Key Location/Qualifiers FH FT source 1..460 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm FT ears; 6. pollen; 7. 1 cm vegetative shoot tips from 15 day FT old seedlings; all leaves with an expanded or partially FT expanded sheath were removed; 8. mature leaf tissue; 9. 0.5 FT cm long root tips from 15 day old seedlings; 10. 10 dap FT whole seed; 11. 12 dap endosperm and embryo; 12. 17 dap FT endosperm and embryo. All of the sequenced clones in Maize FT Full-length cDNA Project will be archived at the University FT of Arizona. Clones, high density filters and amplified FT library can be ordered from the University of Arizona FT (http://www.genome.arizona.edu/orders/)." FT /db_xref="taxon:4577" FT /db_xref="UNILIB:39438" XX SQ Sequence 460 BP; 106 A; 128 C; 134 G; 92 T; 0 other; ctgcttctcc tcctcaagag ctcgtcgcca ccggccaccg ccaccctcga tcacgccgtc 60 cgtcgaggta aggtcagcat ggtgagggtc agtgtgctca acgatgcgct caagtccatg 120 tacaacgctg aaaagatcgg caagaggcag gtcatgatca ggccgtcgtc cagggtcatc 180 atcaagttcc tgacggtcat gcagcgccac ggctactttg gagagttcga gtactttgat 240 gaccaccgat cgggcaagat cgtggtcaaa ctcaacggga ggctgaacaa gtgcggcgtc 300 atcacccctc gttttgatat cggcgtgaaa gacattgagg gatggactgc aagggtgctc 360 ccgtccaggc agttcggata catcgtcctc acaacttcgg caggtatcat ggaccacgaa 420 gaggcccgcc ggaagagcgt aagaggcaag gttctaggtt 460 // ID DY239233; SV 1; linear; mRNA; EST; PLN; 399 BP. XX AC DY239233; XX DT 04-FEB-2006 (Rel. 86, Created) DT 23-FEB-2006 (Rel. 86, Last updated, Version 2) XX DE ZM_BFb0257J22.f ZM_BFb Zea mays cDNA 3', mRNA sequence. XX KW EST. XX OS Zea mays OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade; OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea. XX RN [1] RP 1-399 RA Kim H., Collura K., Wissotski M., Smart D., Kudrna D., Muller C., Rao K., RA Haller K., Wing R., Soderlund C., Walbot V., Yu Y.; RT "Maize Full-length cDNA Project"; RL Unpublished. XX DR MD5; d9f1215d018b3d3750b2c69b8f99f04e. DR UNILIB; 39438; 17667. XX CC Contact: Yeisoo Yu CC Arizona Genomics Institute CC The University of Arizona CC Forbes Building Room 303, Tucson, AZ 85721-0036, USA CC Tel: 520 626 9585 CC Fax: 520 621 1259 CC Email: yeisoo@genome.arizona.edu CC Plate: 0257 row: J column: 22. XX FH Key Location/Qualifiers FH FT source 1..399 FT /organism="Zea mays" FT /lab_host="DH10B T1 phage resistant" FT /cultivar="B73" FT /mol_type="mRNA" FT /dev_stage="varies by tissue" FT /clone_lib="ZM_BFb" FT /tissue_type="mixed (silks, husks, ears, pollen, shoot FT tips, leaf, root tips, whole seed, embryo)" FT /note="Vector: pCMV-SPORT 6.1; Site_1: EcoRV; Site_2: NotI; FT Maize Full length cDNA library (3530 library) created by FT Invitrogen from multiple tissues; Organ: silks, husks, FT ears, pollen, shoot tips, leaf, root tips, whole seed, FT embryo. This is a Gateway compatible vector, permitting FT clone movement to new vector backbones for expression in FT diverse host cells using recombination rather than FT restriction enzymes. poly(A)+ mRNA was prepared by FT Invitrogen, and equimolar amounts of RNA from each of the FT 12 tissue samples were mixed together for selection of mRNA FT with a 5' cap. After synthesis of cDNA, a normalization FT step was conducted against the mixture of RNA sources. FT Tissues prepared: 1. just emerging silks; 2. inner husks FT from ears of sample #1; 3. 20 dap aleurone; 4. immature FT tassels, stages from 1-2 mm to 1-2 cm; 5. 2 mm to 2 cm F