ID EH224871; SV 1; linear; mRNA; EST; PLN; 599 BP. XX AC EH224871; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5444.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5444 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-599 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 733268ff919122bdd622ee42d9493af7. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: g column: 17 CC High quality sequence stop: 516 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..599 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5444" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 599 BP; 131 A; 163 C; 85 G; 220 T; 0 other; aatgcaaact gaaaacggta acaacgctgt cacattatag ttgaggaggc tgtgtataat 60 ttcctgattt tcctcaaatt acagacacaa atagggaatt aagcccaaac acactttaga 120 ctaaataaat agacacaggt tgcccaagcc aatggtcatt tggatccaaa agcaaatgac 180 aattttggtc gggatttgtt tttacccaac aactttattt ggtttagacg ttcttgctcc 240 tcctcctgtt tcttttttcg catggcttct tccttctgtc tttgcatctc ttccagctct 300 ttatatcgtt cttcctccct tctttgctgc tctaaagctt cccttctctg agcttcttca 360 accttcctcc ggttctcctc aagcatcctt tcaagatctt ctttctcttt acgggcttgc 420 tcctccttct gtttagcctc aataagggca gcttcttttt ctttttcaag ttgagctgca 480 acttcatcat taagtctctt ccgtccctct tccaaccggc tttcaatctc cacctgaacc 540 tcctcagatt tcaagctttc ttcaaatctc ttacgtattg cttcttcaag tctctttgc 599 // ID EH224872; SV 1; linear; mRNA; EST; PLN; 676 BP. XX AC EH224872; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5758.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5758 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-676 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 11d9813d267428a55239150f297c8f06. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: l column: 24 CC High quality sequence stop: 616 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..676 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5758" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 676 BP; 246 A; 132 C; 118 G; 180 T; 0 other; gatgaaaagc agcaaagcca atatataagc actccagtca taaaacaaca tagtacacgg 60 taacaaacaa catatggtcc catctatcca ggctcttgta tacatctcaa atgatgaaga 120 atcacatcat aaatcaattt tcggagaaaa accttccatc aataacagtc tcactctgca 180 tctggatatt acctaattgt ttcatctgcc agctctgatg aggggcaaca tgttcgtgcg 240 aaggagtgta gtgctgcaaa aaggaaaagt cagctagaat tcaagttcaa cacggtaatt 300 tgtacacttc ttttatccct caaaggaaat aaggcaaaag agagagaaga gaaagcacgg 360 tccctttgaa atcatttatg cgtatgatca ttttgagagt ttttaaaagt catgtatcct 420 atataagcct tcatgcttac gattgccaca taagcagaaa agtcctggat gacaacaatt 480 actcaacaaa gtgcttaatg cagcaaagtc ctacttgaca actgtcacac aaaaagtgcc 540 aaggggggga gaatttaaga aaaaatatga cctccaatct attctcacta gcccaagtaa 600 taagtcatgc aaagagtttc atgtctatgc attacaatat tattagcatt tataataatg 660 gtagatgttc caatta 676 // ID EH224873; SV 1; linear; mRNA; EST; PLN; 587 BP. XX AC EH224873; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5916.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5916 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-587 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 50cbf1494e6582176ce80d0c2425a1b6. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5916.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: h column: 15 CC High quality sequence stop: 555 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..587 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5916" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 587 BP; 187 A; 116 C; 113 G; 171 T; 0 other; gtggggggtg gtttagactt aagaatatag tttttggatg ctgaaagttg aatacactaa 60 ccaaacaaga atagacatga cacatgtacc caggcttgtg gagtgaacac taaaaaagaa 120 agtaaagaat aatagattta atcaaatagc acaaattagt ggtaattcaa tattgaagaa 180 aatcaagtgg caaaatgttt tatgaagttc attaaggcat tacgcccaaa cccttttcct 240 tgggacaaga tctgatgtct ttatctgatc tatctttgca gcaacaataa aatcattcat 300 acttaaacct ccaatggaag ctgtccatag ttctgctctc acttgatttg gttgctccaa 360 gtagatattt gggaaatggc cctcagcctc tacaacctta gagatcctgt taatgagttc 420 aaccccacat ttaaaatctc tcaatttcca caagcattga attttaagtc cattttcctc 480 attcacaagt ctccaaccca caaccttgag cttgagggcg agccctgatg cagcggtaaa 540 gttgtgcctt ggtgacttgt tggtcatggg ttcgaatccg gaaacag 587 // ID EH224874; SV 1; linear; mRNA; EST; PLN; 658 BP. XX AC EH224874; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5438.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5438 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-658 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 4d95ea1b08d119f08be98065bdc4ba02. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: k column: 15 CC High quality sequence stop: 632 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..658 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5438" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 658 BP; 208 A; 155 C; 101 G; 194 T; 0 other; caaaaaatat tttgtatgtt ctcttcaaaa accgcaactt cacatttgta aagtcatact 60 ttcaattaat tgtttcagtt ttcaatccgc cgcaaagaat ttgtgaaaag gggggaaaaa 120 gaaaaggaaa gaatccctac tacgaacaaa aagtaaaaag aggacggcat cattgcagta 180 aaccggcttc cttgagagcg gctttatatt cttcaacact ttgagctcgc acctcagtta 240 cacaagtgcc aaaatggttt tcaatcttcg acatgagcct ttcatcattt tcatcacata 300 tcaggttaaa tacagccccc ttgcgcccaa aacgcccagc tctgccaacc ctgtgcagat 360 acacttcata atctggttca tcccgtaaac tgttgctgat caaagccacg agcaagaata 420 tctgttgata taagaacttg agtcaaacca tctttgaact ccttgacaac tttgtctctc 480 tcttcattac taagagaacc ttgtatagat gtaacctcat agcccaaatt aacaagtgct 540 tggtgcaata atcttgcact atctcttgtg gccataaata taatagtttg ccccacattc 600 tctcctattt caaatatgta atcttttatc acatcaatct tagcaagtcc tcgggaca 658 // ID EH224875; SV 1; linear; mRNA; EST; PLN; 635 BP. XX AC EH224875; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5915.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5915 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-635 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 74d27b51b13897f949efa682586abffa. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5915.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: f column: 15 CC High quality sequence stop: 562. XX FH Key Location/Qualifiers FH FT source 1..635 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5915" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 635 BP; 201 A; 96 C; 149 G; 187 T; 2 other; acgggatacc aattggaagg aaggttgatt tgagtgccca tagttcctat gaaaccttag 60 cacaaacatt ggaggatatg tttaatgaat caactacagt caccacttgc aaaggatcaa 120 atggtgagga ttatggcatt attatcggag gagaaagaca ttcaaaattg ttggatggat 180 cttccaagtt tgtgctcact tatgaagaca aggaaggaga ttggatgctt gttggggatg 240 ttccctgggg gatgttcctt agctcggtga ggaggctaag aatcatgagg acatctgagg 300 ctaatggact tgcaccaaga ttggaagaaa acatcaaaca gagatgcaag cccatataga 360 tactgttttc atggctaagg acacgaagaa cctgtcaaat agatgaagag aaaaaacaga 420 acagaaaagt aaaggaatat tgcaagtata tgaagttttg agatttgaat gataaaatct 480 cgcacttttg tttaattaat tcagtttgtt tgtttgttta tttggtatat ttgtcgctgc 540 ctctctattc atctcacttg gagggtgcag gcatcataga ttttcatgnc atactactaa 600 tcatancagt cagcctgctt gttagtgttg taaat 635 // ID EH224876; SV 1; linear; mRNA; EST; PLN; 457 BP. XX AC EH224876; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6073.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6073 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-457 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 9703b7a8f210db643c54d63a912182ed. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: b column: 8 CC High quality sequence stop: 456. XX FH Key Location/Qualifiers FH FT source 1..457 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6073" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 457 BP; 130 A; 67 C; 110 G; 150 T; 0 other; catgtctgaa cttgagattg ggggtgcata ttttgaggac tgtaaattgc aaccagcaga 60 atattgttct ggagaaggag ttgtgataaa tgaattccca aagaactcag ggtcagttct 120 tggaacgtcc attaatgttg agaaccctac aaatagttgt tcttatgtgt catcaacaga 180 agaggacatt attgatcttg agctacagtt taaacttgat gaaattgagg cacactatca 240 gcattggatt gatgaactta agaaaatgat gttggaggca ttggattcca ccagaaggag 300 ttgggtggcc aagaagaaat tggctgttca gtgattttga gcttgttgat tgacctcttt 360 ttacccttgt caaaattgtt tcattgcaaa ttttgtaatc ttaacggcaa tttgtatatt 420 ggatgtggct ctttttagtg gaagcgagtg tccttgc 457 // ID EH224877; SV 1; linear; mRNA; EST; PLN; 679 BP. XX AC EH224877; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5443.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5443 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-679 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; f620ddb8ef0f49d70677e66872852fe2. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5443.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: e column: 17 CC High quality sequence stop: 604. XX FH Key Location/Qualifiers FH FT source 1..679 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5443" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 679 BP; 141 A; 197 C; 180 G; 161 T; 0 other; acctgtcgat gtgataagaa aggctgtggc aaaacgcttg tgtttactat ccttttgcag 60 gtaggcttag ggaaggactt ggtcggaaac tgatggtgga ttgtactggg gaaggtgttt 120 tgttcattga ggctgatgca gatgtcactc ttaagcaatt tggtgatgct cttcaacctc 180 ctttcccgtg ctgggaggaa ctcctttatg atgtacctgg ttctcaagga gtgcttaaca 240 ctccccttct gcttattcag gtaacgcggc tcaagtgcgg gggtttcata ttagctgtcc 300 ggctgaacca cacgatgagc gacgcagccg gtctggttca gttcatgagc gcgctgggag 360 aaatagctcg tgggaggcag gagccgtcga tcccaccggt gtggcgcaga gagctcctga 420 acgcgcggga cccacctcga gtcacatgca cccaccgcga atacgaacat gtccctgaca 480 ccaaaggcac catcatcccc ttagaccaca tggcccatcg ctctttcttc ttcggcccca 540 gtgaagtcgc cgccatccgc tccctcatcc cccaaactga tcaacgctgc tccaacttcg 600 aggtcctcac cgcatgcctc tggcgctgcc gcaccattgc gctgcagccc gacaaagacg 660 aggaggttcg catcctctg 679 // ID EH224878; SV 1; linear; mRNA; EST; PLN; 572 BP. XX AC EH224878; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5448.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5448 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-572 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3bf0977e7c705e70309d0d90bd243a73. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: o column: 17 CC High quality sequence stop: 431 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..572 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5448" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 572 BP; 162 A; 133 C; 101 G; 176 T; 0 other; atcaagtcac atgatctttt attcaactta tggaaaatgt taaacattaa gcatattgtt 60 tatgcaaagt tgtgaagtgt actgcaactt aaaacgacat ttttttttta ctgtatcaag 120 gtttgctggg ctttctcaac agaattcctt agtaaggata ttacttgaac tggctcaggg 180 gcaccaaaca ctgaacttcc agcaacaatg cagtttgccc ctgctgatgc ggccacatct 240 atggttgaag gccctaaacc accatcaacc tctatgtcaa gtgatggata cttcttccta 300 agtatacgta ctttatccat catctctggc ataaattttt gtcctccgaa tcccggttct 360 acagtcatca caagaaccat ttccacagga tttccggctt ccaccagagg ataaacctct 420 tcaatggggg tcccaggctt taatgctaca ccaggagtca tgccatgtga cttgattctt 480 tggataagtt cttcccagtt atcttttgat gtctctacat gaaatgtaaa accagaagca 540 ccagcttttg ccaagggctc aacataatca ag 572 // ID EH224879; SV 1; linear; mRNA; EST; PLN; 376 BP. XX AC EH224879; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5789.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5789 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-376 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; a05c577fd2a4cbde1264261c4c67bf8c. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5789.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: i column: 7 CC High quality sequence stop: 375 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..376 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5789" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 376 BP; 102 A; 95 C; 73 G; 106 T; 0 other; aagtacacaa ctaatagcaa aaggtttatt aagctgtaaa ggtgctttta accataaaca 60 aaaatagtcc cccagactat aaaaacatta cattttagac aaattagtct tccacttcat 120 cgaccgtcaa cacctactgt aactgctggg gttgctgcct aacaagtatc atattcactg 180 caaacatctt agtaggagta gcttttgacg aacaactcct tcttggcttc ctcggattca 240 ccctcaccag tgtacttccc aagctgaacc agtgagttgg acttggcacg gaatgccagt 300 gcatcttgtg ctgccttcac gttctctggg cggcctcccc atgtcttcaa ggcagtgttt 360 tggagggctc tggcat 376 // ID EH224880; SV 1; linear; mRNA; EST; PLN; 649 BP. XX AC EH224880; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5441.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5441 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-649 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 0d100afc4766e701259fd862f83dff3a. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5441.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: a column: 17 CC High quality sequence stop: 626. XX FH Key Location/Qualifiers FH FT source 1..649 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5441" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 649 BP; 191 A; 121 C; 155 G; 182 T; 0 other; ctgtagtatc attccttcct cctccttggg tcagggaaaa aactaaagaa ttccctctta 60 caataagaag gtgagatgac agattccttc tcttccagac cactcggttc tactttggtt 120 ctatttgatg tagatggaac tcttacacct gccagacaga gtgtttctgc agagatgctc 180 gagatactga aggccctgag gaacaagact gtgattgggt ttgtcggtgg atctgatctg 240 agcaagatca aagagcagtt agaggtagat cctcaatcga aagtattgca gaactttgat 300 tactgctttg cagagaacgg cttaacagct tacaagaacg gagaggagtt agagagtcag 360 agctttataa agttcttggg cgaggatcgg tacaaggagc ttgtcaactt ttgtctaaag 420 gaactcagtg agatcgatat acctataaag aggggtactt ttatcgaatt cagaaatggg 480 atgatcaacg tctcaccgat tggtagaaat gcttcgattt ctgagaggat tgagtttgag 540 aagcttgatc atcagatcca agtcagatcc aaacttgtct caaagctcaa agaaagattc 600 tcggaatacg atttgacgtt ctctatcgga ggtcaaatct catttgacg 649 // ID EH224881; SV 1; linear; mRNA; EST; PLN; 670 BP. XX AC EH224881; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5467.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5467 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-670 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; a4599f1dc16481ffcee493ebe97f55d2. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5467.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: e column: 23 CC High quality sequence stop: 639 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5467" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 670 BP; 199 A; 146 C; 105 G; 220 T; 0 other; aaggagcaac aaaaagaaag tttggattta ctattaattt gcatcaaatt gtctatctat 60 ctgtgaacaa aatctcaaac tagtagtaac tttttaatct cttcctactt ttctttgctt 120 ataacaaaga caaagaattt tcatttcaag aggaaaaaat gataacaata acaagggaag 180 taggaaactg taaatccgat cagttatgga tgcctcatta ttgtatttta taaactacaa 240 tcaaactgtt tgctagaaga aaaaagaaca gtgacctgtt tgagttttcc tacacgcttt 300 aggttgtagt tgcagcaact gtttgtttcg ttgggcatgc cgcaaatgta tgtcctttct 360 caccacactc atagcatgtc ctcctcctca tcttgccact tcctgtggaa cttgatttat 420 caccatcagc tccatttatc ttgtcacttc ctgtggaact tgatttatca ccattagctt 480 cattaactgt ggagcttgca tgagttccag tttttttctt caaaggaacc gcacaactta 540 tcctgacagg tctcccaaac aaaacatttt ggtccaatgc aagtgctttt tttagtgact 600 gactatcacc aaaatccaca tgggcatacc ctcgaaactc tcctgtctcc ttgtccatac 660 cgaatcgtag 670 // ID EH224882; SV 1; linear; mRNA; EST; PLN; 450 BP. XX AC EH224882; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5765.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5765 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-450 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; fff079e8d8d7feb3d576a167de601b2f. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5765.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: i column: 1 CC High quality sequence stop: 439 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..450 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5765" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 450 BP; 141 A; 97 C; 68 G; 144 T; 0 other; gtgcaaaatg ccaatgcaat tatagtcaaa atcagaagaa caaaatccaa ctagtttcca 60 agcagtgatg tcaaaaatgc caaaacagca ataataatta tgatatttag aaaatagttt 120 gactgtagag gcttatctgg gcttccacca atcgcctcat attcagcccg tatcttctgc 180 ttcataccgt cagacagttc tctctgcaat tcaaataggc acactattga atttattcct 240 ttcttcttca tttaaaactt ccatatttta catgtgatat cacgtgattt gaaaatagct 300 tcttcttgta ttactaaaag atactggcac aagaaaacat gacacttgtt tttattcctt 360 tccagtggag tacttggctg ctacaaactt ctctgcccct gcatttggag aatcatctat 420 cacaatgccc tctggaggat caaatttggc 450 // ID EH224883; SV 1; linear; mRNA; EST; PLN; 744 BP. XX AC EH224883; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5460.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5460 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-744 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; c6c49076a3cfce99042c21db8626d2be. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5460.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: g column: 21 CC High quality sequence stop: 693. XX FH Key Location/Qualifiers FH FT source 1..744 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5460" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 744 BP; 237 A; 135 C; 154 G; 218 T; 0 other; ctgacccaaa cagactataa tgttggcctt gacttcagtt gactttgtct ttattgtagc 60 atttctttcg ttggcatata agtacttcag ttgtgcttca agttctaaca gttcaactag 120 aacaatctct taaccctctc tcaaactcct tcagaatccc cctttcccag ttcccaactt 180 catttcaaat caatatgaag aaaattgtgc taaagttgga aatccatgac gacaaaacca 240 agaaaaaggc catgagggca gtatctggta tttcaggggt ggagacagtc tcagtggaca 300 tgaatgacct gaaaatgact ataattggga atgttgatgc tgtaatagta gttggcaagc 360 ttagaaaatg ttgtgatcat gctgatatac tatcagttgg accagccaaa gaggagaaga 420 aagaggaacc aaagaaagat gagaagaagc cagaagacaa gaaagatcca aaggaggaat 480 atgctgaact tctgaacgcc ttttataatc agaccagaca gcagcaatac cccccttatt 540 atcacagaac agtggaagag gatccaacta gctgtgttat ttgctaagta ttggaatatc 600 atatggagtt tgaaatgatg gataataaaa tgagatcaag aaaagatggt ggctgcatgg 660 agttgaagaa gaattatgtt tcttttggag ttcccttttc cttcttttct ttcttatgtc 720 ttggtctttc agctttcctc ttgt 744 // ID EH224884; SV 1; linear; mRNA; EST; PLN; 450 BP. XX AC EH224884; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5770.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5770 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-450 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 1bf1a0a7489205d7e692ea92807c1324. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5770.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: c column: 3 CC High quality sequence stop: 450 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..450 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5770" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 450 BP; 162 A; 74 C; 92 G; 122 T; 0 other; aaaccaaaat tattgattaa agattataaa caaacatttc tacaatttgt attgaccaga 60 tggaaaatta ctacaatgag aacaaaggtt acaccaacat tacataaaat ccttgtttca 120 tgttcacttt ctcaacaaag agaaacaaca aaatgaatca cacaagggta aaattaagag 180 actttttctt ttctcttctc tcaggcaaca aactcacaat taaccttgtg ataataatcc 240 aaaagtatac aacaactaat ggctaaatta aaaaaaaggt aaaaagaaag atacgaacag 300 tgtttttttg aagtcatcgt tgatggagtt tggttaggtt ttcggcgaat ttggcgagag 360 attggaggtt gcagcggagg atggtgtcga cgaatacgcg cgtgtcctcg gtggtgttgc 420 cgggggggac gtcaacgacg taggactcga 450 // ID EH224885; SV 1; linear; mRNA; EST; PLN; 457 BP. XX AC EH224885; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5777.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5777 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-457 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 36a67c97098b3f068dd85d5e4494693a. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: a column: 5 CC High quality sequence stop: 457. XX FH Key Location/Qualifiers FH FT source 1..457 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5777" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 457 BP; 99 A; 96 C; 121 G; 141 T; 0 other; cggtttgttg ttgtaactcg ttcttcttct tttgttgctc tgtgtttgtg tttgtttttt 60 ctctcacctg aaaatgtctt gctgcggtgg taactgtggt tgcggaagct cctgcaagtg 120 cggcaacggc tgcggaggct gcaagatgta cccagacttg agctacactg agtcaaccac 180 caccgagacc ttggtcatgg gagtggcacc tgccaatgct caattcgagg gtgctgaaat 240 gggtgtgccc gctgagaacg atggctgcta atgtggacca aactgctcct gcaacccctg 300 cacttgcaag tgaggtgttg ggagagcaag cttcaagcat agatagacct taattaggaa 360 taatgataaa ctattaatgt gtagctcaca acttatgtat tatgtctgtg atggttttcg 420 tgcactctgg tgttttgtgt taaacggaca tccttag 457 // ID EH224886; SV 1; linear; mRNA; EST; PLN; 331 BP. XX AC EH224886; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5452.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5452 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-331 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 977fd01e13ea1ef7c4065efed3873dd1. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5452.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: g column: 19 CC High quality sequence stop: 225 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..331 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5452" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 331 BP; 107 A; 74 C; 67 G; 83 T; 0 other; cattcttgag atctataatg taaacctgtg ccatagtagt tacagaaagc ttatttacaa 60 cacactaaca agcttattaa aacatgccct atataatctc ctagatgtct tgttaggcag 120 ctttggcgaa accaactcgg aggttcccat agtcaaagac ggtgtgatat accctcatga 180 aaacatcgcc aagaatccag agaggacctc gaggaggagg aatgtcaaaa gcaataaacc 240 cactaaggca aacttctgca atgccttctc cagttttcag aatatactgc tcaggggtga 300 gggtgcacgg tttgtctcca acagtaaacg t 331 // ID EH224887; SV 1; linear; mRNA; EST; PLN; 566 BP. XX AC EH224887; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5785.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5785 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-566 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ef7354aa817ee031dcb493a20078ac33. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5785.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: a column: 7 CC High quality sequence stop: 487 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..566 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5785" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 566 BP; 155 A; 158 C; 114 G; 139 T; 0 other; aacccgaact caaaattatt atttaagctg aaacaaactc gattaattat tagtgcaaca 60 aataaactat atatatcaaa acccttaatt aagcttctct gaatactata gcaactccgg 120 ataaccgatc cattcagtgc ttctcaatga actccttgat cctagaaatg agagcatcgg 180 tttgagcact aacattgggg tccatatcca cagcaatctt attcagataa aaactatgtg 240 tcatcccttt gctaacataa agttccacat ccttgttggc cttcttcatg gcctcatagt 300 actccatctc cgtgtccctc accaagtcca tctccgccac gcatagcagc accggaggca 360 gtttcagccc ttcgagtggc ggtgccgcct cacccatcgg gcacgtaaag gggtggtcct 420 tggtggcccc aacaggaagc gccaaggcta agaacttgtc cagcatgtcc aacgttaaaa 480 acggtgtctg tggcatttcc atctcggacc ggctccggtt ggaccggaca aaaccggggt 540 gaaccggaat tgcccccgcg acccgg 566 // ID EH224888; SV 1; linear; mRNA; EST; PLN; 554 BP. XX AC EH224888; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5923.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5923 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-554 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b2c2ed9b420b4e2c2078ad17227dee66. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5923.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: f column: 17 CC High quality sequence stop: 467 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..554 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5923" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 554 BP; 150 A; 109 C; 100 G; 195 T; 0 other; ggatgctatc agaatctatg caattgcatg tacattagtg gaaaaggtaa tacgaaatat 60 atttattaga atatacatat ttttctttat ttgcacagaa ctggattaac atctttgttt 120 ttccactcta tcaatgttga aatttgacta gaaaggatat cagaaatttt ctaagtgctg 180 gctttggctc tctttactgg ggcctgaagg gaatttagat aaatggcatc ctttttgtcc 240 agtccatcaa acagataact atacggtggc atttggatgt atctcccagt tccaggagtg 300 acctttagtt cattattttc ccaaacaact cttcctccca caatagtcac ttcaattttt 360 ccctttcctc ttcttccctc atagacattt gtgtccagtc tggagtggtg ggactttgca 420 ctcatctcaa agctcgaatt tggattaagg atgataatat ctgcctcaga tcctgggaga 480 atagctcctt tccttggata tatattgaag attttagcac attcagtgct tgttatccgg 540 acatagtcag tgac 554 // ID EH224889; SV 1; linear; mRNA; EST; PLN; 654 BP. XX AC EH224889; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5453.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5453 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-654 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 1ac93492a2625f6e533d4c8f7ef2b237. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: i column: 19 CC High quality sequence stop: 654 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..654 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5453" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 654 BP; 206 A; 112 C; 172 G; 164 T; 0 other; aataaaagaa gattaattga tattagccag aattacatca gatattcaga tgttacatga 60 aaaattatat cagatctaga caatttcaga tcatgtcctc tgataatact atcaaggacc 120 taaaaaacaa aaaagaagag aaaatcaacc tatttacatc aaaggatgta ctaattaact 180 tcttaatgga agattaatct tccaaaaaaa aaaagagaga aaaaaaatct ccttagtcct 240 ttctttctgc tatctttttt tatgaaaaaa taaagagata aaaaaaaggg ttaatattca 300 ttaggcggag ccaagttaag atcgagattg agctctcttt tgggctgaca atcgacgacg 360 acggaggagg agtcggagtc gctctggacc ggaccgggtt cgaaccggag cgggaaagac 420 tgggtcatga agtgggcccg gtcgagaaag aaaaccgggc gcatgggtgc tgcgacgggg 480 tgggtcatga ggatttgctg ctggatgggt aggaacggga aacggtcgat tgcggcggag 540 gtggagcggc gcgtggcttc acgctccggg gtggcggact ccacggtgct gctctggctg 600 ggactgtgag atttaacgtt aacatcttgt ttttgttcac gttaacgttg ttgc 654 // ID EH224890; SV 1; linear; mRNA; EST; PLN; 559 BP. XX AC EH224890; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5455.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5455 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-559 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 8e7de127d634ec23a0534ce403957ca5. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5455.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: m column: 19 CC High quality sequence stop: 552 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..559 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5455" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 559 BP; 154 A; 155 C; 92 G; 156 T; 2 other; agcgcaacca ttgacataat cccataacgt tctacgaact tggttaactt acaccatcca 60 cacactccga acccatttct tacacttcca ttctcaccca cattgccctc aaacacaaaa 120 caaatcctaa ccagaagcag attttatgaa gaaaatagct accctattag tagtgctcta 180 agaacagtct cccacggaac atgcttcatc ttcattcaca ttgccttctc cgtgtttaaa 240 cgccttagca atgtcatcaa ttggaatcag aggcaaatag tcaaacacgg cataaccctt 300 ctcacttgtt cccttgacca cattgttgta tttttctgac cagtgtgcag ctgtgggaac 360 tgccatctca gccactgcat ggaatggtgg atagtggttg agaccagtcc acaatttcac 420 tgaaccaaag agcacaattt gcttggactc agtggctaca taatttgctg ctgctcgtgc 480 ccctccagat tgagcttcac tcactacttt ctcagctttg tgtgtcactt cctgaaccaa 540 acaagtgact tggcttgnn 559 // ID EH224891; SV 1; linear; mRNA; EST; PLN; 534 BP. XX AC EH224891; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5779.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5779 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-534 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 4af96fb6f6e63d8ebac64b874dfc400f. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5779.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: e column: 5 CC High quality sequence stop: 295. XX FH Key Location/Qualifiers FH FT source 1..534 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5779" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 534 BP; 127 A; 197 C; 126 G; 84 T; 0 other; ctctctctct ctctacaaca agaattcatc tattcatttc agcccatcag catggcgaag 60 gagaagaaaa acttgtccaa caagaacaag aagccaagca atgcagacga ggttcacttc 120 cgcggagtcc ggaagcgccc ctggggccgc tatgccgccg agatccgcga ccctggaaag 180 aagacccgcg tctggctcgg caccttcgac accgccgagg atgccgcccg cgcctacgac 240 gtcgccgccc gaaacttccg tggccccaaa gccaaaacca acttcccctt cctccccgac 300 aacaacacct acgtgaacct ctacaagaag aacagcaacg ttgacgtgaa atctgacagt 360 cccagccaga gcagcaccgt ggagtcctcc accccggagc gcgaattcac gcgccgcgcc 420 gcccccgccg caatggatcg ctttccggtc ctacgcatcc ggcagcaaat cctcgtgacc 480 cagcccgtcg cagcagtgcg cccggtgtac tgtctcgacc gggcccactt catg 534 // ID EH224892; SV 1; linear; mRNA; EST; PLN; 631 BP. XX AC EH224892; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5780.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5780 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-631 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e5c7a7899f54fd448aefbb622e2fa59a. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5780.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: g column: 5 CC High quality sequence stop: 570. XX FH Key Location/Qualifiers FH FT source 1..631 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5780" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 631 BP; 173 A; 136 C; 151 G; 170 T; 1 other; ctcggtcctg ttgtgtgtgc gcagaataaa atttctcatc gacgatggcg aataccttac 60 aacgcgccct tcgcgccacc atggcccggc cgttctccac cggcgcattg gccgaaatta 120 agcgaggcga gatcggaatg gtttctggaa ttcctgaaga acaccttcgt aggagggttc 180 taatttactc tcctgctaga actgcatctc aacaaggatc tggaaaggtt ggaagatgga 240 agatcaactt cttatcaacc caaaagtggg aaaatccact gatgggctgg acatccactg 300 gggaccccta ctctcatgtt ggtgattctg ccttgacttt tgatagcgaa caagcagcaa 360 aagcatttgc agagaaacat ggttgggagt attcggtcaa gaagccccac acaccattgc 420 tgaaggttaa atcatacgcg gacaatttta aatggaaggg cccccccaaa tctggtgaag 480 agtgatttgt tggtttgtat tcatgttaat gtattccgtc ttccggagtt tcaacgtgca 540 gcagtttttc tctgcatatt ttcttggaaa aagaaagttt aatgtgaatt actgattgaa 600 aataaaaggg actgatctgc cattggntaa a 631 // ID EH224893; SV 1; linear; mRNA; EST; PLN; 622 BP. XX AC EH224893; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6070.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6070 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-622 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 54e702ef8b6d1ce7a4a6205fbb322ac6. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: l column: 6 CC High quality sequence stop: 567. XX FH Key Location/Qualifiers FH FT source 1..622 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6070" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 622 BP; 202 A; 108 C; 144 G; 168 T; 0 other; aaatttagaa ttggttctgg aaatcatctc tcagagtcca gaggaagaat taaaggagtt 60 gctttcaaaa caaaacaact cttgtgaaac tgccttatat gttgctgctg aaaatggtca 120 tcttgatata ctcaaggaat tgattagata ccatgatatt gggttggcca gcttcaaagc 180 tagaaatgga tttgatgcat tccacattgc tgctaaaaat ggacacttgg agatattgaa 240 agtcctcatg gaggcctttc ctgaaatttc aatgactgtt gatctgtcga acactactgt 300 gttgcatact gctgcagcac aaggacacat tgaggtagta aattttctct tggaaaaagg 360 taatagcctg gtaactattg caaaaagcaa tgggaaaact gtgttgcatt cttctgcaag 420 aaatggctac atggaggttg tcaaggccct tgtgagcaaa gaaccagaaa ttgcaatgag 480 aattgataaa aaggcgcaga cagcactcca tatggcagtt aaaggacaga atcttgagtt 540 ggtggatgag ctcgtgaaat tgaatccatc tgtggccaat atggtggata ccaaggggaa 600 cactgcactg catatagcaa cc 622 // ID EH224894; SV 1; linear; mRNA; EST; PLN; 677 BP. XX AC EH224894; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5608.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5608 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-677 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 78098b72c7dafa375032755246092188. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5608.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: o column: 10 CC High quality sequence stop: 613 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..677 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5608" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 677 BP; 212 A; 178 C; 137 G; 150 T; 0 other; atcattatcg ctgaacaatg ttatttaaca aaagtatcaa ccacaaataa aaacaacaac 60 ccaacttccc aatcatcgtc acgtccagca aaatacattc aaaagctatc taaaatctgg 120 ggaacccaca ttggcattaa aaaccatgac catacaattt tttagagaaa tattatgggc 180 accatgcctc ttcaacaatc ccctaaacgt tgcttaggca tcagcaaacc caagctcgga 240 aagcttttgg tgagcctcag cgtaatcagc aaagaaggca tcttcgtccg ctgcatattt 300 atcaacgaga gggcggaata cagggtcaga caaaagagcc ttgtcagaag gtagctgaag 360 gagaccttcc ttctcaccac tcaacaactc cgtgaagtat gagttgtcga aaataagagg 420 attagaggtc cagggaccct caaatccaga acgctccttg tgtgcagctc caatagtgtg 480 acccccagat agagcaacga tatcttggtc agtaagcccc atagctttgc caaacacatc 540 tctcaaatgg tcagaaccct tagtggcatc gggcaagcga ccctctggtg gtggctcagg 600 cttgtcctct cttccagggt ggaatggaac ttcaggtcca cccgtgacct caacggcaac 660 aacgccagcc aactggt 677 // ID EH224895; SV 1; linear; mRNA; EST; PLN; 494 BP. XX AC EH224895; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5609.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5609 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-494 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; dde9202def4cc5e3e4030629641179b9. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5609.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: a column: 12 CC High quality sequence stop: 442 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..494 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5609" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 494 BP; 141 A; 130 C; 114 G; 108 T; 1 other; aagtaaaaga aaagcggata tagcattaga tacaagttgc aggaagcaag ttacatgaaa 60 actattagta tgatcatatg atgataattg atatcccaca gcatttaatt aagccccttc 120 cctatttaaa tttcacaaac attaaatcac tgggatctca tcctgttgag ctggttaaga 180 gcaggctgca agatgttgta aagaacccaa gcaatagccg gcgtaaccac aaacagcaga 240 agctgtcccc tgttgtcgtt cgaggcagct tcagccatcg ccgccacttc agaagccgcc 300 gccgccgatg catgcggcgc agccaccaag ccagccaagc cgccaagccc caacccaatg 360 atcacgcctt tggtgctgcg catgcggttc agttggttca gcgccggctg cagaatgttg 420 aacagaaccc ancctattgc aggaattatg ggcagcaaca gtgccagacc gcggttgtcg 480 gcttctgcta tgtc 494 // ID EH224896; SV 1; linear; mRNA; EST; PLN; 779 BP. XX AC EH224896; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5614.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5614 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-779 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 79b1dd4776dea7e9b4f1c0b832cde054. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5614.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: k column: 12 CC High quality sequence stop: 651. XX FH Key Location/Qualifiers FH FT source 1..779 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5614" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 779 BP; 218 A; 148 C; 191 G; 222 T; 0 other; cttcgacctt ctttcacaaa accaaatcat cacctaagtt cagcatgagc atgagcaagg 60 atccggttcg tgagtggatt ctttctgatg ggaaagctac ggagataacc aaaattagcc 120 ctgttggtgg gggttgtatc aatcttgcta gtcgctacga caccgatgct ggttcattct 180 ttgttaaaac aaacaggagt attggaccat ccatgtttga agctgaggct cttggtttag 240 gagctatgta tgaaaccggg actatccgtg tacctaagcc ctataaggtt ggaccgctac 300 ctactggtgg ttctttcatc attatggaat tcatacaatt tggtgcatct agaggctatc 360 agtctgatct agggaggaag cttgctgaaa tgcataaagc tggaaaatct agtaaaggct 420 ttggtttcga tgttgataac accattggca gcactccaca agtaaacacc tggtcatcag 480 attgggttca attttatgga gagcatagat tgggttacca gttgaagttg gcattagacc 540 agtatggtga cagaactatt tatgacaaag gacagagact ggtgaaaagc atggcaccct 600 tgtttgccaa tgtagtgata gaaccatgct tactacacgg agacctctgg agtggaaaca 660 tcagttctga caaaaatgga gaacctgtca tattggaccc tgcatgttat tatggacaca 720 gtgaagcaga atttggtatg tccttggtgt gctgggcttg gaggatcatt ctataattc 779 // ID EH224897; SV 1; linear; mRNA; EST; PLN; 699 BP. XX AC EH224897; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5467.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5467 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-699 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 72f31ccce17bd21dc25ea673cfa7f093. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5467.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: e column: 23 CC High quality sequence stop: 672. XX FH Key Location/Qualifiers FH FT source 1..699 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5467" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 699 BP; 241 A; 150 C; 155 G; 153 T; 0 other; gttttgtgac tgtgtctgtc gagggtttga gagagcagaa tggttctatc aaacaagaaa 60 ctgaagctta agctgagagc tgaattagcc caaagcaact ccaacgccgc cgctgaaccc 120 ccttcatttt ctattaataa taataactct ctcaaaactc ttctggactc tgccacgccc 180 acacccagat tcttcaagag agagaagcga agaaagcttc gatccgacca acctccacaa 240 caacccaaca aggaaaatga aactgaaaca cagggttcgc ccaataagaa gacaaagagg 300 aagaagctag aggacgatga cgtggcagca actggagttt ccgacacacc aaagaagaag 360 aaaaacccca ggacggctaa ggaaaacgaa tctaatggtg gaggggaaat gccagaggct 420 acagagttaa ccaagaccaa tacaactacc agtcaagaca atggtgatgc tcctaataca 480 aatacaaaga tttatgttgg aggaattccc tattattcaa ctgaggatga tattcgaagt 540 tattttgaaa gctgtggcac cataactgaa gttgattgca tgacttttcc tgaaactggc 600 aagtttagag ggattgccat tattactttc aagactgaag cagcagccaa aagagcattg 660 gctcttgatg gagctgacat gggaggactc tttcttaaa 699 // ID EH224898; SV 1; linear; mRNA; EST; PLN; 465 BP. XX AC EH224898; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6074.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6074 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-465 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; af056c92129a24d8626ed1b150c3695d. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: d column: 8 CC High quality sequence stop: 465 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..465 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6074" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 465 BP; 148 A; 109 C; 81 G; 127 T; 0 other; aacaaaaccc tgattttata cgaacattac ttgggtatag ggaatcaaaa aaataaggga 60 aaacattaat tcaagcttaa cacaaaattg accaaaaata aggctctcaa ctccaaaaag 120 tatccaaatt ttggacatag aaaataaaca gtacaggcaa ataacatagt ttctacttgt 180 gcagctatga aacagcatcc gcaatgaact ttcactcatc atcttcatca ttatcagcag 240 cagagttgat ggctgccacg cgtgcaacca acttagcctt cctctcctca aaagtcagct 300 tcttcatgtt gtaccccttg ggctccttcg ggggctgctt atcaaatttc ctgagagtag 360 gatcagcacg gatggcagca tgaaccttct tgtaaagtgc ctcaatacca tcagcctcta 420 tcccacgttt gatgtattcc ctgaagtgtg actggtattt ctctg 465 // ID EH224899; SV 1; linear; mRNA; EST; PLN; 478 BP. XX AC EH224899; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5496.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5496 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-478 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b2993ae6c55818480878f4c2ce3370cf. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5496.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: p column: 5 CC High quality sequence stop: 478 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..478 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5496" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 478 BP; 135 A; 65 C; 121 G; 157 T; 0 other; agtaatttat tgtgggattc tattctaaga ttttgagaat gtctagctgt gggtgtggaa 60 gcagctgcaa ctgtggcagc aactgcggtt gcaacaagta ctcttttgac ttgagttacg 120 ttgagaagac aaccacagag actctagttt tgggtgttgg gcctgtgaag gcccaattgg 180 agggtgctga aatgggtgtt gcatctgaga acggtggctg caactgtgga tcaagctgca 240 cctgtgaccc atgcaactgc aagtaaggtc aagatgtata accttgaagc agagatagct 300 gtaatatatg tatcaacaaa taataagaag tagaagttgc gtttggagct ttagaataaa 360 gtgtttgcta tgtgttttgt tggtctttta taaatatgta atttgaatca tcaatctctg 420 cttataaatg aatacttttg gtgttatatg aataaaaagt ttgttgggtt atttcatt 478 // ID EH224900; SV 1; linear; mRNA; EST; PLN; 474 BP. XX AC EH224900; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5795.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5795 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-474 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; bf4de516d43e8eb4d936d47863c981de. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: e column: 9 CC High quality sequence stop: 456 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..474 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5795" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 474 BP; 131 A; 117 C; 91 G; 135 T; 0 other; aaaaagtact aaagttattt cattgaatga ttgtaagtta aaataacatt tattttgagt 60 ttttttttct aatacaattc tccgtaacta ttgcgaaacg gaaggagtaa atctcaataa 120 tggttcacga atacgtcgta ttccacgata gcgtctcctt tggtgaaatc aacagagtaa 180 aatctggctt gagcgtagcc acgtgcaaaa caaaaagccc cagtgccgcc aacaatagcc 240 atttccctaa caggttcact catgatcatg ttcctcccca acacgctgat ggtgctgccg 300 ttgaaatctc catcggtgaa tgccatcgtc ctcaccatca ttagtcccat ctcttcttgc 360 gatattgaag tgtaaattcc ttgggccttg cccacaagtt tcgagtcctg ttcgggcccc 420 acggttaaag ggtcgtccat cgccacaacg gttccgaacg gaagggcatc cttc 474 // ID EH224901; SV 1; linear; mRNA; EST; PLN; 741 BP. XX AC EH224901; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5457.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5457 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-741 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; f3b759b9157523b0d202e71433033708. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5457.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: a column: 21 CC High quality sequence stop: 715. XX FH Key Location/Qualifiers FH FT source 1..741 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5457" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 741 BP; 191 A; 147 C; 179 G; 224 T; 0 other; atccaacctg acataaaccc aaatgcacat aggagcaaaa actcacgtag agagaggacc 60 agagttcagc ctcctctctt gcctggtctt ccggatgatc ttgctattgc atgtttgatt 120 cgtgtacctc gggttgagca tggtaaactg cgtttggttt gcaagagatg gtatcacctc 180 ctatctggaa atttcttcta ttcactcagg agaagtcttg gaatggcaga agaatgggtt 240 tatgtcatca aaagagaccg tgatggaaga atttcattgc atgcttttga tcccatctac 300 caactgtggc aatcccttcc tcctgttcct ggggaatatt ctgaagcatt gggatttggc 360 tgtgcggttc tcagtggttg ccacctgtac ttatttggtg gaagagatcc actgaaggga 420 tctatgagac gtgttatttt ctacaatgcc cgtactaata agtggcatag agcaccagat 480 atgctgcgga aacgtcatct gtttggttct tgtggtaata aataactgtc tctatgttgc 540 tggtggggag tgtgaaggaa ttcaaagaac tcttcgatct gctgaagttt atgaccccaa 600 ccggaacagg tggagtttta tctcagagat gaccacagca atggtgccct ttattggtgt 660 tgttcataac ggaacatggt ttttgaaagg acttggatct aatcgaaatg tcctatgtga 720 atcctattcc aggaaactga t 741 // ID EH224902; SV 1; linear; mRNA; EST; PLN; 288 BP. XX AC EH224902; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5464.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5464 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-288 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; f1a8500531fcb054070abf10f9e0384a. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5464.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: o column: 21 CC High quality sequence stop: 260 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..288 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5464" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 288 BP; 90 A; 69 C; 25 G; 104 T; 0 other; tttttttttt tacgaacaaa actcataata attattcaat caataaagta tacacaccac 60 caccaaccaa cctttttccc cacaaggtgg tataggctac atgaattaaa taacagcata 120 gcttctatta taaatcatgt ctacagacaa actattgatc tataaattca tttctatagc 180 tttgtatata tttcttctcc gttccccttt gcctataatg atttaggcta ccctccatgt 240 gaactaccct tcttcccaag acttttatca gtcctcttaa ttctcaat 288 // ID EH224903; SV 1; linear; mRNA; EST; PLN; 560 BP. XX AC EH224903; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5620.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5620 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-560 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ee2cf2f4563f55fb8f674b9ebe9c6b6c. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5620.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: g column: 14 CC High quality sequence stop: 519. XX FH Key Location/Qualifiers FH FT source 1..560 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5620" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 560 BP; 164 A; 149 C; 95 G; 152 T; 0 other; ccttctccaa cgtcctctct catccttctc agccaactcc gaaggaaacg ctcttcatgc 60 tttgagaagc aggctttctg accccagcaa cgtgcttcag agctgggacc caaatctcgt 120 taatgcttgt acttggtttc atgttacctg cgactccaac aatcacgtca ttcgcttaga 180 cttgggcaat tctaagctct ccggaacttt gggaccagaa cttgcccagc taccccacct 240 ccaatacctg gagctctaca ggaataacat aagtggcaac attcctcggg aactttctaa 300 attgaagaac cttattagta tggatttgta tgacaaccaa tttcatggca aaatccccaa 360 gtcttttggc aacttgaact cacttaaatt tctacgccta aacaacaaca aactgacagg 420 agcgatccca agagagctca ctcatcttaa aaatctcaaa atccttgatg tttctaataa 480 tgacctctgt ggaacaatac cagtagatgg caactttgaa tcctttccga tggaaagttt 540 tgagaataac aaacttcagc 560 // ID EH224904; SV 1; linear; mRNA; EST; PLN; 225 BP. XX AC EH224904; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5622.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5622 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-225 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; da5ae04d86fefd2f586919d669bf47a8. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Inverted Insert: Based upon the presence of a run of 14 or more T CC residues at the beginning of this sequence, this clone probably CC contains an inverted insert. The resulting Poly-T sequence has been CC removed. CC Plate: ABWZ 0057 row: k column: 14 CC High quality sequence stop: 213 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..225 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5622" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 225 BP; 82 A; 18 C; 47 G; 78 T; 0 other; gttaacgcta aaaatagttc agtcaaagat ttaaatgtaa aagaaagaaa gaaagagaga 60 aagaaagaaa actgatttga ttcgattttt tgaatagtta cactgcactt tgatagtctg 120 taagggatat aacaggttta taacatagtg agaaaatgtc aatgtgatat ttgattctgg 180 ttattttttt cgcttttgaa tgaatattgt tttgagtggc atgtg 225 // ID EH224905; SV 1; linear; mRNA; EST; PLN; 499 BP. XX AC EH224905; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5934.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5934 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-499 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 2dd3c981bbd5c510441ff13a59f78505. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5934.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: l column: 19 CC High quality sequence stop: 499 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..499 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5934" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 499 BP; 129 A; 80 C; 118 G; 172 T; 0 other; tgtcacttgc atggcctcgt acaaggtgaa gctcataacc ccagaaggag aagtggagtt 60 tgaatgccca gatgatgttt tcattgttga caaggcagag gacgaaggca ttgaacttcc 120 ctactcgtgc agggccggtt cttgcgtttc ctgtgttggc aaaattgtgg aaggtaacgt 180 ggaccagtca gatggtagct tccttgatga tgagcaaata gatagtggct gggttctcac 240 ctgtgttgct caacctaggt cagacgttgt cattgagaca cacaaggagg gagagatcga 300 atgatcaata tatatatata tgatgtaatc ttatccattg ctgttctaat tctatctata 360 tcttggattt tttattttgt tatagtgatt tggcatattt gtgacaaata gtgagtttat 420 tacttttgtc atatttgtga aaacaagtga aattgttgca ccttaattca ttagtttggt 480 gatgcttttc ttactgttt 499 // ID EH224906; SV 1; linear; mRNA; EST; PLN; 691 BP. XX AC EH224906; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5640.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5640 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-691 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e7549d5e1c759fd2b5c2d76288ec4ecf. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: o column: 18 CC High quality sequence stop: 662 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..691 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5640" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 691 BP; 197 A; 158 C; 169 G; 167 T; 0 other; cattagttct catccattaa tttccattga agtccgaaaa cagaaaccaa aaatacagga 60 tgatgaaatc tcaaaaagca aaaacaaatg ggaatgtagt ttaagtacaa acaaacaaag 120 tggggtggcg gctacaaatg ctttgcaaat ttttcacact tagaagcctg ggggcttgta 180 ggcgatgaag ctgatgcact gcacttggcg aacgttgtcg aatccgatga tacggatgaa 240 gccgttgggg tatgcagtct tagcctcttg aagctccttc aacacctgag aagcatcagt 300 gcaaccaaac ataggcagct tccacatggt ccagtagcgt ccatcgtagt atcatggtga 360 cctgttgtgc tcacggtaca cgaaaccgtg ctccaactcg aattccaagc aaggaatcca 420 tcccttccta agaaggtatt ctacttcctt tgccaattgt gcatcatcaa ggtctggcag 480 gtaggaaaga gtctcaaact tcttcttgcc aactggtggc cacacctgca tgcattgcac 540 tcttccaccg ttgctagcaa tggaggtaat gtcattgttg gtctttctgg tggggaagcc 600 agccatggac ttgaggccag tgaatggagc aaccatgccg gcaccggcac ggttgacagt 660 ggtaacagct ggggaagaga tcattgagga a 691 // ID EH224907; SV 1; linear; mRNA; EST; PLN; 699 BP. XX AC EH224907; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5469.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5469 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-699 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3ac5b94f3cee54e5b828d913b5bbd66f. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5469.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: i column: 23 CC High quality sequence stop: 664. XX FH Key Location/Qualifiers FH FT source 1..699 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5469" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 699 BP; 155 A; 182 C; 164 G; 198 T; 0 other; tagcttcact gttagggcca tccaatcaga aaagccaacc tatcaagtga ttcaaccaat 60 caacggtgat cccttcattg gaagccttga aaccccagtt acatccagcc ccttgattgc 120 atggtactta tcgaacctcc ccgcatacag gaccgcagtg agcccactac taagagggat 180 cgaggtgggc ctggcccatg gctaccttct ggtgggccca ttcgtgaagg ccgggcctct 240 gaggaacacc gagatcgccg ggcaagcggg ctctctcgcc gctggtgggc ttgtggtgat 300 cctcagcctt tgcctcacaa tctatgggat ttcatctttt aacgaaggag acccatccac 360 tgccccgtca ctgaccttga cgggccgcaa gaaggagccc gatcagctcc aaactgctga 420 tgggtgggcc aagttcaccg gaggcttctt cttcggaggc atttcgggtg ttatttgggc 480 ctacttcctc ctctacgtct tggaccttcc atactacatc aagtgaagaa agtgcttatt 540 atctttgttt actttttgtt gttattcatt ctctatgaat ggacaatggg tggaaatgga 600 tccttatttg tgcaatagag atggatgtaa atgatgtatt gtatgactct caacatgtta 660 caatgttaaa ttctctctct cctgttctgg ccttctttt 699 // ID EH224908; SV 1; linear; mRNA; EST; PLN; 605 BP. XX AC EH224908; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5477.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5477 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-605 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; c6098c38ebb3db325d10601cd3f3638a. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: j column: 1 CC High quality sequence stop: 567. XX FH Key Location/Qualifiers FH FT source 1..605 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5477" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 605 BP; 169 A; 131 C; 157 G; 148 T; 0 other; aatcacaaaa agacctacaa ggtacctcgt aggccattcg aggccccgcg tcttgatgct 60 gaacttaaac ttgctggaga attcggttta agaaacaagc gagaaatatg gagaatcgcc 120 ttgaccctct caaaaattcg tcgtgctgct cgtgagctgc ttaagctgga cgacaaggac 180 cccaaacgac tatttgaggg caatgcattg attcggcggc tcgttcgaat tggggtttta 240 gacgagtcca ggatgagact tgattatgta ttagccttga aagttgaaga ttttatggag 300 cgaaggttgc aaactcaggt cttcaagtct ggccttgcca aatctattca ccacgctaga 360 gttttgattc gccagcgcca cattcgtgta ggaaaacaga tcgtcaacgt cccttctttt 420 gttgtacgcc ttgacagcca gaagcatatt gactttgccc tcactagtcc gtatggagga 480 tctcgtgctg gacgtgtaaa gagaaagcgg gcaaagaagg ccaacgctgg ggaggaggca 540 gcagaggatg atgaggaata gacaagttaa atgtcatgtt gcaattcaga cgccatccac 600 aagtg 605 // ID EH224909; SV 1; linear; mRNA; EST; PLN; 504 BP. XX AC EH224909; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5631.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5631 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-504 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 912d97771325233f1d9471352e8e50d9. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: m column: 16 CC High quality sequence stop: 408 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..504 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5631" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 504 BP; 168 A; 117 C; 92 G; 126 T; 1 other; gggaagtaat tcttacataa caaattcctt aatgattcac aagcaacttg acattaagac 60 tatccttaaa caagtattca aaactgcaca tcagtactta cacataaaat agacactgct 120 atgaatctat caaatataag aagagtaaag atgcagactt tataagttca aacaaaagaa 180 gcatttcctg aaaaaaacct gacacacata ctatttgtta tttacaactc caagaaagct 240 taattctgtg aggcagtggc ctgaaggaga ccatcccgaa tttgcacggc aatgtcttta 300 actgccccag gaccgtgagg tatgaacaca gacgaggacc ttgacgatgc tcctatctct 360 ttcatggtgt caaagtattg agtcaccagc accatgtcca tgacatcttt agctgatgtc 420 ccgggcacgt tctcagagaa agcaagcacg ctgtccctca gtccatccac aatggnctga 480 cgtttgacga gcgataccga gtcc 504 // ID EH224910; SV 1; linear; mRNA; EST; PLN; 281 BP. XX AC EH224910; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5804.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5804 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-281 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 8efede8a4817c902da6200c0a49e2dc3. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5804.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: g column: 11 CC High quality sequence stop: 281 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..281 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5804" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 281 BP; 107 A; 45 C; 49 G; 80 T; 0 other; gaaaaaagca ttattattta ttcaaaagga aactctcttc caaccatttt acatatctgt 60 taagtcacca aacattttgt cacaaaggtc atcaaattaa aatcataaaa aaaaaatgaa 120 attctacata gattgcacaa cttcatagtg cccgaaatag ataaatttga agcttgactt 180 agcaacttag cgtccaacac tttgaaggct gttagtgaag ggtactgatt gcaaggaatc 240 ataagggggt tgtgcatgcg tatacgtgtt taaagaaaga g 281 // ID EH224911; SV 1; linear; mRNA; EST; PLN; 649 BP. XX AC EH224911; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5471.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5471 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-649 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b584feba5c739bea69b05af248b0ac42. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: m column: 23 CC High quality sequence stop: 586 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..649 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5471" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 649 BP; 233 A; 122 C; 66 G; 227 T; 1 other; gaggtggcaa aaagaaatgc ttaattaaat atcaaaatat acaatatata aaatatacaa 60 caattacttt actggataat aatattattt tacaaaaaaa ctattaattc agagaaagtg 120 attcaaaata aggatgtatt tattgttaat actctccaca tgtatattat ttaaaaatgt 180 ttttttttaa aaaaaaaaat ataaaggtaa acatgttatt tatcttttct atcctcattt 240 ggcaaaaccg tatttaactt agccatataa ttctttacta tttttacttc cataatcagc 300 caataaaatt tgaaaaaaaa agtatccctc atgttaaaaa aaaatattcc gttcaaaagt 360 aatcatagtt ttcttcattc ttataattcc acttaactat tcgcgaaaaa ttatgatatc 420 atcgataatt ccaaaagatt tggcaccttc aggatccata aatttatcac gctctaaagc 480 tcgctcaaac ctctcatgcc tctcttctac attctcttct ttcatgccgc agtgtcttcc 540 gtaaatctca atcaaccttt ctttcagcct cattatttcc tgagcacgaa tcgncatatc 600 tgatgctttt ccactaaatc ctccggaagg ctgatgaatc atgaccgtc 649 // ID EH224912; SV 1; linear; mRNA; EST; PLN; 281 BP. XX AC EH224912; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5627.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5627 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-281 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 008b62d62408a88504b41b7c1306d877. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: e column: 16 CC High quality sequence stop: 245. XX FH Key Location/Qualifiers FH FT source 1..281 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5627" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 281 BP; 63 A; 78 C; 82 G; 58 T; 0 other; ggccggaaaa tacgctgagc gtgtcggcgc cggcgcccct gtctacctcg ccgccgtact 60 cgaatatctc gcggctgagg ttttggaatt ggctggaaac gctgcgagag acaacaagaa 120 gactcgaatt gtgccgcgcc acattcaatt ggcggtgagg aacgacgagg agttgagcaa 180 gctactcggt gacgtcacca tcgctaacgg tggcgtcatg cctaacattc acaatcttct 240 ccttcccaag aaggctggtg gatcatccaa ggctgctgct g 281 // ID EH224913; SV 1; linear; mRNA; EST; PLN; 694 BP. XX AC EH224913; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5476.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5476 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-694 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 5b8205aefa3bd37905a4dc3c25b87870. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: h column: 1 CC High quality sequence stop: 649 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..694 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5476" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 694 BP; 210 A; 139 C; 141 G; 204 T; 0 other; gagaatacca aattacttca gtcaacgact gaagcacgtt ctaaattgtt agataggtaa 60 atgatcaaca catatattca tacaagttcc aacataaggc agcttccaag agaagaaatt 120 aacaggtaac tggaagctgc taggaatatt aattcgaata gttcatacat aaattcctgt 180 agatttccaa tataaaatgg ttacaattgt tattttggag ccataagttt gaagcctatc 240 atcaggcgcc actagcatat aattgggtcc attccttcgc tgtttcgaca gcctcggcct 300 cattagattt ccaatgcttg gcaatgttct cagaaagtgg atcatctggg tttggtgcac 360 tcaatagagc ttgaatgctt aatagaacag tgcgtatctg aagtgcagga ctccacttat 420 ctttcagaat atcaagacat atcctgccaa gcttatcaat gttcggatga tatatttttg 480 ttagaaacct aacctttgga gcagccattg gatattcttc tggcaaaaat agttctaact 540 tgaaaacgcc tccttcataa ggcgactggg taggcccaag gatcatcaca ttgaaatatc 600 gcatattctc ttcagagggg gaggcactaa taccaggtgc tggctcactg agcaaacgct 660 gcgtttcctt gatgattctt cgagggaggt tgct 694 // ID EH224914; SV 1; linear; mRNA; EST; PLN; 695 BP. XX AC EH224914; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5482.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5482 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-695 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e704b753d990d31dd8e8d6747756aa5e. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: d column: 3 CC High quality sequence stop: 693. XX FH Key Location/Qualifiers FH FT source 1..695 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5482" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 695 BP; 173 A; 169 C; 153 G; 200 T; 0 other; ctcacacgca aaagagagag agaatcgaaa caccccgtaa gcagcacggc acaatcgcaa 60 cgcaagagat taataatcaa tttgcatcga tcgatcgatc gttggttagg tttttgtttt 120 gggggaatat gggcgagttg actcaggcgg aggtgtactc gcctcggact cagcaagtgt 180 ggagggcact gttgagttgg ttggccttct tctttcagat cttcttccag attataagag 240 ccttaggtca ctaccctcta ctctctttct cttctaattc ttcttcttct tcaaacgctt 300 cttcctcttc ctcttccttc aaacctcttc cctccgttga gattgctgaa cacgattcgc 360 ctcctccctc cgccctcgaa atctcaccac ttgattattc ccccgccgat catcccgtcc 420 caaaactcac ggttgtgctg gacttagatg aaacactggt atgtgcatat gagacatcaa 480 gtttgccagc tgctctacgg actcaagcaa tcgaaggtgg tttgaattgg tttgagctgg 540 aatgtgtatc gatagacaag gaaggagaag gcaaaaaaaa atcaattatg ttacagtatt 600 tgaacgccct gggttgaagg aatttttaaa gcaactcagt gaatttgctg acatggtgct 660 gtttacagct ggtcttgaag gctatgctag acccc 695 // ID EH224915; SV 1; linear; mRNA; EST; PLN; 522 BP. XX AC EH224915; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5634.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5634 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-522 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 6e63bd4a63975a0a989556970f3db6c3. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5634.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: c column: 18 CC High quality sequence stop: 479 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..522 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5634" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 522 BP; 181 A; 115 C; 75 G; 151 T; 0 other; aactttttaa cttaccatag ttcaattatt ttgtggacat catcatgcta agatgttcac 60 tcatgacaac acatacaaca aataaaatcg ccaacttctt tcaagtaaca tagtactgtt 120 tacactaaac atgcaaatac tactacagca acttgaatac acattattat ccatatttta 180 aacaagatta ttacacatga aacagtcacg gccattggtc taacatttat agaaagaggt 240 cataggagca aattgaacga aaccagcttc agttaacatc aactcgagga acctcaaact 300 ttagatttgg aatcatagtt tcaaatgctt cccatgaacc aggtcatgtc ttaggagcta 360 atagaatgaa gccagtttaa gttaacctcg actcgaggaa cctctaactt cagatttgga 420 agcatagctt cgaatgcttc ccatgaagca gctgtctctt catcacatac caccaccttc 480 aaattctcaa gattggttac tgagtatggc aattcacacc tt 522 // ID EH224916; SV 1; linear; mRNA; EST; PLN; 373 BP. XX AC EH224916; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5636.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5636 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-373 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e4359efd5f0d077054727d825fea7aaa. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5636.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: g column: 18 CC High quality sequence stop: 373 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..373 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5636" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 373 BP; 143 A; 75 C; 60 G; 95 T; 0 other; ttaaaagaaa aacacatctt ttgggaagta attcttacat aacaaattcc ttaatgattc 60 acaagcaact tgacattaag actatcctta aacaagtatt caaaactgca catcagtact 120 tacacataaa atagacactg ctatgaatct atcaaatata agaagagtaa agatgcagac 180 tttataagtt caaacaaaag aagcatttcc tgaaaaaaac ctgacacaca tactatttgt 240 tatttacaac tccaagaaag cttaattctg tgaggcagtg gcctgaagga gaccatcccg 300 aatttgcacg gcaatgtctt taactgcccc aggaccgtga ggtatgaaca cagacgagga 360 ccttgacgat gct 373 // ID EH224917; SV 1; linear; mRNA; EST; PLN; 671 BP. XX AC EH224917; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5634.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5634 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-671 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 79a2ae8307d73fb1dd6017448db4c8da. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5634.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: c column: 18 CC High quality sequence stop: 622. XX FH Key Location/Qualifiers FH FT source 1..671 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5634" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 671 BP; 227 A; 139 C; 116 G; 188 T; 1 other; tcatcctcca tgatcttcta agagagcttg caaactatca aaacaacctg gaaccaattg 60 aaaagagaaa aagactgatt aatgacataa atgaatccga ggagaagcaa caaggcatga 120 tagcccgctt attgtcaaaa ttttgcagat gcagtgttaa acagacactc caacaggtcc 180 ccgcccgcac attgtctata tcagctgatg aaacaaacac ttcatatcag tcccacatac 240 aaccatccct agctgaagtt ctggttttaa atcttcaaac taagaagtat tcctttccag 300 agtacataga gaaaatgagt gaactaaaag ttcttatcat gacaaattat ggttttcatc 360 cgtgtgaact agacaatttc aaactactca gctctgtatc aaacctgaga agaatcagac 420 tagagcggat ttctgttcct cacttagggg cattgaaaaa tcttggaaag ttatccctct 480 acatgtgtag taacataagt caggcttttg aaaatggtac catcacagtt ttggattcat 540 ttccaaaact atcagatttg aacattgact attgcaagga tatggtgaaa ttgcctactg 600 ggatctgtga tattgtctca ctaaagaagc tcagtattac taattgtcac aagcttagtt 660 ctctgnccca a 671 // ID EH224918; SV 1; linear; mRNA; EST; PLN; 732 BP. XX AC EH224918; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5635.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5635 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-732 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; f0a332a0742885525aa77d0be8dc44f2. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: e column: 18 CC High quality sequence stop: 689. XX FH Key Location/Qualifiers FH FT source 1..732 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5635" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 732 BP; 223 A; 146 C; 181 G; 182 T; 0 other; gattcattct catctttctc tcttctctct ccacccaaac cctaactccc gaccctgttc 60 ccctctcgta tccgtcatga aaggaggaaa gtccaagacc gaatctaaga gagccgatcc 120 caaacttgct gtgaataaga agggagccgc caccaaggct aggaaacccg ccggcaaggg 180 gaaggcagcg aaagacccta acaagccaaa gaggcctcca agtgctttct tcgttttcat 240 ggaggagttc aggaaggtat tcaacaaaga acatcctgaa aacaaagcag tgtctgctgt 300 gggcaaagct gctggagcaa aatggaagac catgtctgat gctgaaaaag caccttatgt 360 ggcaaagtct gagaaaagga aagtggagta cgagaagaac atgagagcct ataacaagaa 420 acaggcggaa ggtcctactg gtggggatga agaagaatct gagaagtctg tatctgaggt 480 gaatgatgaa gatgatgatg aggaaggcag tggcgaggaa gaggatgacg attagagaat 540 ggactgataa ttgcatttat gtaggttctg atgattttga tcatctgttg taggtggtct 600 ggtcatttcc tgataaacat atttcttgct tccaaaaaat atttccctgc tccctccgta 660 aattggttgt ctttccaaaa cgtgaatagt tttaagaagg gagtctttat ttgtactttc 720 taatcaactg tt 732 // ID EH224919; SV 1; linear; mRNA; EST; PLN; 526 BP. XX AC EH224919; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5479.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5479 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-526 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; c3c6af0d85e1b4a9527d2520b0fa01e8. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5479.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: n column: 1 CC High quality sequence stop: 488 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..526 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5479" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 526 BP; 125 A; 135 C; 130 G; 136 T; 0 other; gataaaaact tcaataaaaa gttctagttt gcttgggtaa cagcaacaca tccaccaaat 60 ggaccagcgt ttgcagcatt tctgcatcgt accaagcatg tttggccgtc agcaccacca 120 gtgcaggtag cgccagcggg catggttgca acaagtggaa attcagtagc ctttgccctt 180 gatcgactgt tcttgccagg aacgtttgtg tcgatattca ttttcttgaa gttgtcacca 240 gtggcggtgg tgtcaacttc acagctgtat gggcctccac cgtcaccgtt gacctggtga 300 agagtcattc tgacttttcc gtcagggcca atgctggcca atccggcaga ttcagccgat 360 gacatgtcac tagcaatatc gttgtttccg cccgcaagag tgcgtccaca agcagacgcc 420 tttccgcttg aaacctcacg gtctcgaatg atggagctgt cagtttggaa tgggttcgtt 480 cgagtaccat cacgaggagt ggactctacc attccaaagg cagatc 526 // ID EH224920; SV 1; linear; mRNA; EST; PLN; 721 BP. XX AC EH224920; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5809.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5809 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-721 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; f2c994d700dc26e8e6691c2c9faff641. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5809.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: a column: 13 CC High quality sequence stop: 612. XX FH Key Location/Qualifiers FH FT source 1..721 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5809" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 721 BP; 189 A; 191 C; 176 G; 163 T; 2 other; ctctctgttc tcttctacct ctcaagtttt tgaagtatag agatggcaga gacattccta 60 tttacctcag agtcagtgaa cgagggacac cctgacaagc tctgtgacca aatctctgat 120 gctgtcctcg acgcttgcct cgaacaggac ccagacagca aggttgcctg cgaaacatgc 180 accaaaacca acttggtcat ggtcttcgga gaaatcacga ccaaggccaa tgttgactac 240 gagaagatag tgcgtgacac ctgcaggaac atcggctttg tctcaaacga tgtgggactg 300 gatgccgaca actgcaaggt cctcgtcaac attgagcagc agagccctga tattgctcag 360 ggtgtacacg gccacctcac caaaaaacct gaagaaattg gtgctggtga ccagggtcac 420 atgtttggct atgccactga tgaaacccct gaattgatgc cattgagcca tgttcttgca 480 acaaaactcg gtgctcgtct caccgaggtt cgcaagaacg gtacctgccc ttggctgagg 540 cctgatggga agacccaagt gaccgttgag tattacaatg acaatggtgc cagggttccg 600 gttcgtgtcc acaccgtgcn tatctccacc ncacacgacg agactgtcac caatgacgaa 660 attgctgctg acctcaaaga gcatgtgatc aagcctgtga tcccagagga gtaccttgat 720 g 721 // ID EH224921; SV 1; linear; mRNA; EST; PLN; 567 BP. XX AC EH224921; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5941.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5941 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-567 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 4b49bf252a29ddd4eaf69dc2b7cd3ef8. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5941.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: j column: 21 CC High quality sequence stop: 501 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..567 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5941" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 567 BP; 157 A; 115 C; 138 G; 157 T; 0 other; aagagcagtg gattatcatc attgtcacat atgatgaagc aatattcaac aagtgcaaga 60 actgtatgaa taggaataag agtttatgta caacgggaaa tgaaaatagt tgtcatttga 120 acacattatg ctgtggcagc tgcttgttct tcaacagcct gtgatgctgg gagaagggtg 180 caaatgaggt tgcggtcacc gtacacatta tcaacacgtc ctgtggtagg ccagaacttt 240 gcagtacgga gccaaggagc tgggaaggct gcatattccc tggagtatgg ttttgtccat 300 gcatcagcca tgagcagtga tggtggatga ggggcgccct taagtacatt gttgttaatg 360 tcaacctttc ctttctcaat ctcagcaatt tcttgtctaa tggaaataag agcatcacag 420 aacctgtcta actcggcctt gctttcactc tcagtaggct caatcatgag tgtgccaggc 480 acaggccatg acattgttgg tgcatgaaaa ccgtagtcca tgaggcgctt tgcaacatct 540 tcaggctcaa ttcagcagta ttcttaa 567 // ID EH224922; SV 1; linear; mRNA; EST; PLN; 637 BP. XX AC EH224922; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5942.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5942 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-637 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; c718a2b3fdca4475a60bb01c0a82bd20. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5942.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: l column: 21 CC High quality sequence stop: 537. XX FH Key Location/Qualifiers FH FT source 1..637 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5942" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 637 BP; 210 A; 104 C; 150 G; 172 T; 1 other; gtgttgatat ctctaatttg cgctattata tgtagaattg cttttgggag aaggtacgaa 60 gatgaaggaa ctgaaaggag taggttccag gagctactca atgagtgtga ggccatgttg 120 agtatcttct ttgtttcgga ttatgttcca ttcttgggtt ggattgataa actgagtgga 180 ctgcaagcac gtcttgagaa aagtttcaag gagttggata agttttccca agaggtcatt 240 gaggaacaca tggatcccaa cagaaggact tcaaaggaag aggatataat tgatgtctta 300 cttcaactga agaagcagcg ttcgttttca acagacctca ctattgatca catcaaagcg 360 gtgttcatgg acttgcttat agcagcaacg gatcccactg cagcaacaac agtttgggct 420 atgaccgaac tagtaagaca cccaagggta atgaagaaag ttcaagaaga aattagaaac 480 ctaggaggta agaaagattt cttagaagaa aatgacattc agaagtttcc ctatttncag 540 gcagtgataa aagagacatt gagattatac ccaccagtgc cactcctttt gccaaaagag 600 acaaatgaaa attgcattat agatggttat gaaattg 637 // ID EH224923; SV 1; linear; mRNA; EST; PLN; 458 BP. XX AC EH224923; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5821.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5821 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-458 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b172c65ca95fa53f368f0515319b3751. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5821.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: i column: 15 CC High quality sequence stop: 458 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..458 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5821" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 458 BP; 133 A; 79 C; 107 G; 139 T; 0 other; ataagatgaa gaagatggtg gttgcaatga tgcttatatt tcttctaatt tctacgcaaa 60 tggagagcgt tgagcccgat gctgcagatt gcttggatgg ttgcactact gcctgtgttc 120 aaagcgactc gaggcttcaa gcacggtgtg atcgcaagtg cagcattaga tgcggtccag 180 attccacaat caaggaggac atgggttgag tatgtaaagt cacagcgcaa gcaagcagtg 240 tctgcttgac caacaccgtg tcttttttta gtccttgctt atatatgata taacatttta 300 aagtcttccc gatgctgtag tagcatccaa ataaacatgg atgatagaca gctttttaag 360 atcaaatttg ttagggtaga tgctttgtaa cttgaaggaa tgtgatatga atgaatgttt 420 cattatggca ataaatacat cactgccaat gtgactat 458 // ID EH224924; SV 1; linear; mRNA; EST; PLN; 615 BP. XX AC EH224924; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5812.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5812 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-615 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 1fecfada21904532ba8d9b83427949c6. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: g column: 13 CC High quality sequence stop: 592. XX FH Key Location/Qualifiers FH FT source 1..615 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5812" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 615 BP; 212 A; 95 C; 191 G; 117 T; 0 other; caatggtgaa actggtgaag gtgaagacgg acgacctcga agagcatttg atcgtcgcag 60 tgggactgga cgaggaaatg aattcaaacg tgaaggttca ggacgaggta actggggaac 120 ccaaactgat gaacttgctc aggtaactga tgaggttgtg aatgaaacgg aaaagaattt 180 gggtgatgag aagcctgcag ttgaagaaga tgtagctgat ggaaacaagg acagccctac 240 taatgaaacc gaagaaaaag agcctgaaga taaggaaatg actctggagg aatatgaaaa 300 agtgttggaa gagagaagga aggccttcca agcactcaag actgaagaga gaaaggtgga 360 cactaaagag tttgaatcca tgcaggcctt atcaagcaag aaagataatc atgacatatt 420 cattaaactg ggatctgata aagataagcg aaaagaggct tttgagaagg aagaaaaatc 480 caagaagtcc gtgagcatca ctgagtttct gaagcctgct gagggggaag catactataa 540 cccagggcgt ggtaggggtc gtggccgtgg ttcacgaggt ggaggcggag gcggaggtgt 600 ttaccgtgga agtcc 615 // ID EH224925; SV 1; linear; mRNA; EST; PLN; 644 BP. XX AC EH224925; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6101.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6101 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-644 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 46cd5cc4d5ec2d2e51cd38af55c016b5. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6101.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: j column: 14 CC High quality sequence stop: 576 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..644 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6101" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 644 BP; 210 A; 148 C; 105 G; 181 T; 0 other; gataaacaac ttggagccgt ttcctgataa caggataact aaaaccaaaa attcaattac 60 acaactataa gacacaagca actaaaagaa aaagagttaa ctcatgaatt aactcaataa 120 attaacctat aattacaagc tataatatct atttattgct ttacacagtt accatatcac 180 ttatgatagg ttttccagaa atgacaaggt tgctaaactg gccgcccaga catcattgtc 240 tagcacatgc tcttgtggag aatgacttat tcctccgcga caacgcacaa acagcattcc 300 cacctttgtt aagtgagata ttgccattgc atcatgccct gctccactca ttaacgttgg 360 cacttcatct tgaatgtcac cctccatctt cttgagtgca gaataagctg cagacttgag 420 ctgtgaactc agatcagaat cacaaatcac agcacctgca tcatgcttgt gttcaataat 480 acaggaaacc gaacgcttgt cacatatttg gtaaatttgt ttagataaat catagataac 540 agcctcgcgt ccaaggtcat caattgctcg tatatccacg gtatatgtaa cctggcctgg 600 gatgacatta ctggcacttt ggcatgttga tatctctcca acag 644 // ID EH224926; SV 1; linear; mRNA; EST; PLN; 176 BP. XX AC EH224926; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5493.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5493 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-176 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 7238ce0b7c93cebbc50cfa884733e94d. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5493.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: j column: 5 CC High quality sequence stop: 173. XX FH Key Location/Qualifiers FH FT source 1..176 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5493" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 176 BP; 53 A; 32 C; 54 G; 37 T; 0 other; caggtggatc gtgcactggg aaggctaagc ctcttgcacc tggtgaagtg ggtggaaaat 60 gtgcacacga atataatgca tgagcaacag gtgacgggct ttaaccgtgc agtggaaagg 120 gcaacaaatg gatcagagtt gaggctaatg gatatcactg aagcctttca atacag 176 // ID EH224927; SV 1; linear; mRNA; EST; PLN; 384 BP. XX AC EH224927; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5820.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5820 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-384 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 0440abccb5128473f1f6f263a6e4d63a. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: g column: 15 CC High quality sequence stop: 384. XX FH Key Location/Qualifiers FH FT source 1..384 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5820" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 384 BP; 112 A; 62 C; 73 G; 137 T; 0 other; cgatggaaga gctgttttga ttcatccaaa ttttgaaatc ttgctgtcat ttgataaaat 60 gtaagccgaa gtattgctgt caattaagaa gatgtaagca tagccagcca ctgtaattgt 120 atcagtattt attctttcat gtaggttggt tttcctgtcc tgctcatcac gtggtataga 180 aatgtttctt cagctgtctt aacaaaagaa agttattgtg agagacgctg aggcgctgca 240 aagatagcat gaaaaggcaa tcgtagtaat tttgatccac ataattctta ttatgatcaa 300 ttgtcaattc cttgttctct aacttccttt tggtttgtct tagctttcaa atataagcaa 360 aatccgtata tttcaaaagg gtgt 384 // ID EH224928; SV 1; linear; mRNA; EST; PLN; 663 BP. XX AC EH224928; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5494.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5494 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-663 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; f70182422339f7f9afa2be3d9357dcc6. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5494.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: l column: 5 CC High quality sequence stop: 634 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..663 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5494" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 663 BP; 178 A; 140 C; 115 G; 230 T; 0 other; gtgcgaccaa ttttctcagc catgtatgat gaagaagcag agttggccat ataacatgta 60 ttttaacttt aagcataagt atgcttgagt tatataagtg gacattatcc accatctata 120 cagaaaccat ttagatcatg ggacaagaca tttgcaaaag gtgcctagtt aatccaagat 180 ttctagataa aatgtaaagg ctttcagcta tttgatcaaa aactttgatg gttgctctct 240 tcgatttctg caggctgctt ttacgcttcc aagcgcttga caaatttggt cattgatttt 300 gttccatctt tctttttttt tccttttaaa cttgtatggc ttatatagca tacaccttca 360 ttgacagcat tacattcttc agtcaagaat tttctctccg gttgaaaaga tattccagac 420 caagtttgtc tgcccatatg ccaccaaggt gatgctcgtc cttccaaaag aatggcttct 480 ccagtccctt cagttgattg cagtggatcc atctttgttt ctgaaatgtc atactcctca 540 gacttgtaac aaacgcggaa cttatttgtt ttaccaactt cagtttgtgc atgctttctt 600 ttgctcaaac caagtcgata tacttcccca tgtccaacat tgttggtgcc ataaggagct 660 gag 663 // ID EH224929; SV 1; linear; mRNA; EST; PLN; 585 BP. XX AC EH224929; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5642.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5642 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-585 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; f3a909ba82672f3fb91e4f689a98506d. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5642.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: c column: 20 CC High quality sequence stop: 553 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..585 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5642" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 585 BP; 208 A; 122 C; 125 G; 130 T; 0 other; aaactaaaaa gtgtgtttta atgcagtcaa aaaaaatgta gcatatcgtt ttaactgtca 60 ctcttaggag cgtcattaat gatgaacacc cagagaagtt gagcgaaata aaaaaaagca 120 aaatgagcat ggccgctaga ccaataaaca agaaggtcac aagctagaag agaaaaaaaa 180 ttaggaaaac aaaaaaccct catttctaca aagtgctttt tgataaagcc ttataacctt 240 taaaaaggaa aggatgattg agaagaacta ctagaagagg atgaagtcat gaaacctccg 300 gggaacgtag tagtaaacga agatacactg ccacaactaa aaccgaaagg tgttctaaca 360 catccactgc aagctcctgg accacaaaaa ccgcctgctg gacatcccgg accgtcacaa 420 cgtagtaagc ccccaaccgg tggtactcca ggaacgcctc caagaggtat accgccagcc 480 acaacaccaa cgtgaggtat tggtggtcga cctataggtg gtggtggggg tattactttt 540 acgggcctct tggtgcatgg tttcttcttt ttccaatgat tttta 585 // ID EH224930; SV 1; linear; mRNA; EST; PLN; 697 BP. XX AC EH224930; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5495.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5495 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-697 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ccd9f894e84490d94cd3ed0499d13ece. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: n column: 5 CC High quality sequence stop: 639. XX FH Key Location/Qualifiers FH FT source 1..697 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5495" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 697 BP; 200 A; 148 C; 175 G; 174 T; 0 other; cggaagagag cgttaaggtt gtaagactta acattgaaat atattatttc agtcatcaag 60 aacaagtcaa ttagaaatgc ctcttgctcc ccggaatcac aaaaagacct acaaggtacc 120 tcgtaggcca ttcgaggccc cgcgtcttga tgctgaactt aaacttgctg gagaattcgg 180 tttaagaaac aagcgagaaa tatggagaat cgccttgacc ctctcaaaaa ttcgtcgtgc 240 tgctcgtgag ctgcttaagc tggacgacaa ggaccccaaa cgactatttg agggcaatgc 300 attgattcgg cggctcgttc gaattggggt tttagacgag tccaggatga gacttgatta 360 tgtattagcc ttgaaagttg aagattttat ggagcgaagg ttgcaaactc aggtcttcaa 420 gtctggcctt gccaaatcta ttcaccacgc tagagttttg attcgccagc gccacattcg 480 tgtaggaaaa cagatcgtca acgtcccttc ttttgttgta cgccttgaca gccagaagca 540 tattgacttt gccctcacta gtccgtatgg aggatctcgt gctggacgtg taaagagaaa 600 gcgggcaaag aaggccaacg ctggggagga ggcagcagag gatgatgagg acagacaagt 660 taaatgttat gttgcaattc agacgccatc cacaagt 697 // ID EH224931; SV 1; linear; mRNA; EST; PLN; 500 BP. XX AC EH224931; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5496.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5496 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-500 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e0c74bf5b744d8ae1e1917427c9ee886. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5496.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: p column: 5 CC High quality sequence stop: 500 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..500 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5496" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 500 BP; 166 A; 122 C; 71 G; 141 T; 0 other; aatgaaataa cccaacaaac tttttattca tataacacca aaagtattca tttataagca 60 gagattgatg attcaaatta catatttata aaagaccaac aaaacacata gcaaacactt 120 tattctaaag ctccaaacgc aacttctact tcttattatt tgttgataca tatattacag 180 ctatctctgc ttcaaggtta tacatcttga ccttacttgc agttgcatgg gtcacaggtg 240 cagcttgatc cacagttgca gccaccgttc tcagatgcaa cacccatttc agcaccctcc 300 aattgggcct tcacaggccc aacacccaaa actagagtct ctgtggttgt cttctcaacg 360 taactcaagt caaaagagta cttgttgcaa ccgcagttgc tgccacagtt gcagctgctt 420 ccacacccac agctagacat tctcaaaatc ttagaataga atcccacaat aaattactga 480 agaactaaag atgattggtt 500 // ID EH224932; SV 1; linear; mRNA; EST; PLN; 281 BP. XX AC EH224932; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5957.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5957 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-281 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 70d5db24709b805898039d3a4185c8d7. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5957.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: i column: 2 CC High quality sequence stop: 243 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..281 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5957" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 281 BP; 98 A; 57 C; 50 G; 76 T; 0 other; cacttgaagt taaaactcat aaagcacaaa aagatcaata cgcctatgct taatttcaat 60 cagcgatagt taagcaaaat aacaaggtaa ataaagtagc tataataaat tttaaattcc 120 gcatttgtcc tttatacttc tgctactcat gcggcagcat tcgctgggtc acgcttagac 180 gcaaagtccc acgtaataga gtctttcaaa aacccattgg ctaactcttc cgggaaggat 240 tcatcgcagt aaacgccttg tgagatagaa agatttgaga g 281 // ID EH224933; SV 1; linear; mRNA; EST; PLN; 665 BP. XX AC EH224933; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5649.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5649 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-665 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ac37cabc17e819a2458d9eea0f26caaf. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5649.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: a column: 22 CC High quality sequence stop: 598 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..665 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5649" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 665 BP; 207 A; 124 C; 127 G; 207 T; 0 other; gataagaaaa tatgcgatgc cagtatggat aaagctagag aaagagaagc caattattgt 60 ctcaatcttg tatcaacata actgaatctc agcctcactt ttttttctcg gataagtaca 120 tacatacata catatgtgtt tttttcatta attatgatgc agaacagcct aataacaata 180 gacaaaataa catgaaacac agtgaaattg tggaaaacat gaaagaagaa agaaaatgat 240 gattttgatg agaactcatg caatttgact gcgttgctcg gtcgatatgt atatatatta 300 ttatggcagg ttcaattcaa ttcctaagct tttgcagcca ctggagtctc agccttggtg 360 gctattagct gctcatataa atctcttatc ttcaaaccaa ctatggtttg gaagagacct 420 gttccattgt tgctgccggg gaaatcagca tgcttcaaca tctgttgagt aacctctcca 480 tagaactttg tagagaggtt gcttaggtgg ctctcaacgt atattagttc atctagtctg 540 tcaaattgtc catccatttc tacaactgac acctcatttc cgtagtaagt ctctggtcca 600 tagaagaatt tgatgccagg ggtaggagca tgttaagctt ccttcccaat ggtatccatg 660 aaatg 665 // ID EH224934; SV 1; linear; mRNA; EST; PLN; 602 BP. XX AC EH224934; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5953.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5953 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-602 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 0366fb6a7a0030c57751ac2e38a8ddf8. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5953.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: a column: 2 CC High quality sequence stop: 368. XX FH Key Location/Qualifiers FH FT source 1..602 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5953" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 602 BP; 130 A; 188 C; 138 G; 146 T; 0 other; cactcgctag ccacaaacca gttccatttc atccattcca actttccaac cacaacacac 60 tgtgtgtttg taaaaatggc catcacaggc aaccttaact tcaacctcaa cctagtactt 120 cctgtggcac gccatatggc gtgccctcca cgcgtcccgt cgtttctctt cactcgcggt 180 cgaggcgcgt ggtccccctc gcttctggtc acgcgcgcca tcgacgctaa cgagtttctg 240 ggggatttcg gggcgcagga ccctttcccc gccgagcttg aaagctcgtt cggggaaaaa 300 gtgctgggat acggcaacac tgagcacaag attcttattc ccaccattac tgctctcggt 360 ctctcgcagc atgagtgtgc tcccgtttct tctgcacagc cccccatctc gctggatgac 420 gcccagactc tcattataaa gctatgggag agggatgttg tacgcagcct tacccttgca 480 tatgcaaaga ggctgtttcc ggattcgaac ccatgaccaa caagtcacca atgcacaact 540 gtaccgctgc atcatggctc gccctcaagc tcaaggttgt gggttggaga cttgtgaatg 600 ag 602 // ID EH224935; SV 1; linear; mRNA; EST; PLN; 443 BP. XX AC EH224935; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5503.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5503 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-443 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b84017f0a05852caba3cb4e4fb6f1efe. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: n column: 7 CC High quality sequence stop: 443 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..443 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5503" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 443 BP; 137 A; 126 C; 75 G; 105 T; 0 other; tgattaaata aatgtgcacc aagtacaaaa agatgattgg tctttctaac cacgaatgac 60 ttaagaccat tttggtgtgc attcatgact gaattacacc aaggtaccac ccttaacata 120 ctcagtaaaa gccagagcaa ccaaacccaa catagcaaat ctcccattcc acagttctgc 180 atctgacgac atgaaccctt tggacttaga ctccacgctg atcccttgga acagtggaat 240 caaggaagca agggtaagga ccacacttgt tcccaagaac catgggatac caccattgga 300 tatttgttca aacacacctt gccctttggc tacttccact gccattgcac ccacaaaccc 360 aatcatggcc aaccttccat tgatcctctc aggtgctggc ccactgaacg ccaacacatc 420 agaaaacttg gtgctcacct ttg 443 // ID EH224936; SV 1; linear; mRNA; EST; PLN; 782 BP. XX AC EH224936; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5498.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5498 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-782 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 1dfd1eb5760075cfb48dd0d312693e8c. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5498.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: d column: 7 CC High quality sequence stop: 683. XX FH Key Location/Qualifiers FH FT source 1..782 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5498" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 782 BP; 217 A; 153 C; 200 G; 211 T; 1 other; cactgagcta atcggtacag gtaaagctga tcttggctgc aaagccatga ttcatacttt 60 ggctggaaag gccagaggtt ttcctatcaa gagtatcgga actctgatgg acgaaccctt 120 caccggtgtg atatacttag aaggttcagg tatcacttct gactttaagt ctctcaaggg 180 taagagaatt ggatacgttg gtgaatttgg gaagattcag atcgacgaat taacaaagta 240 ctacggaatg acctctgatg attacacggc cgttagatgt ggtatgaacg tctcaaaggc 300 catcatcgaa ggtactattg atgctggtat cggacttgag aatatccaac aggtcgagct 360 ggaggagtgg tgcaaatcta acggccgacc agctagtgat gtcaaaatgt tgagaatcga 420 cgagcttgcc gagcttggtt gctgttgctt ctgctcaatt ttatatatat cgctaacgaa 480 gattggttgg cagctcatcc caaggaagct tccgcattta tgcgtgctgt aaaggccgga 540 gcggatgata tgtttgctga tccacgtggc agctggttag agtactgcaa atttaagcct 600 cctatgaaca ctcccttgaa tcgattgatg tttgagcgta gctttaacta catgagcaag 660 gacttagcaa atgtggctcg tgactggaac aaggtgacca antactcaaa gcgcttggga 720 attgttccaa cagacttttg taagcaacta cacaacgagt ttgtccaatg ggaagtagct 780 ga 782 // ID EH224937; SV 1; linear; mRNA; EST; PLN; 584 BP. XX AC EH224937; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5972.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5972 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-584 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e020fd16d92dd436e37f9d0601623000. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5972.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: g column: 6 CC High quality sequence stop: 563 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..584 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5972" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 584 BP; 207 A; 142 C; 95 G; 140 T; 0 other; aaaccataaa cagtttataa aacttcgtta taaattaaat gcaacttaaa acatccctca 60 gcagcaaagg aaatggacta aaaccctata gctgcttgtg aaattcgaaa tatctcagac 120 tcttcttacc atcaaaacaa aaaacagtac tcatcaaaaa caaacagcaa tgaaaattga 180 ccaccgccga tcaagaacct agttttgaaa gttacggcta catgaaatcg gcagaaactt 240 aaaaatataa cttaaacaaa acacctaaga ctctgcaagg ataaaactac tataaaggag 300 taaccacgat gatgatcaca tgtgctagca aaattgccca aatgcaatca cttcttcttg 360 gcagcggcct tggtgacctt ggctccggtg gggtctttct tctcaacact cttgatgact 420 ccgacagcca cagtttgacg catgtccctc acagcaaaac gaccaagggg aggatactca 480 gagaaagttt caaccaccat gggcttggtt ggaatcatct taaccatacc tgcatcacca 540 ttcttcaaaa atttgggctc cttctcaagc tccttaccag atcg 584 // ID EH224938; SV 1; linear; mRNA; EST; PLN; 617 BP. XX AC EH224938; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6114.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6114 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-617 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; c11796beec8294df1c6bbb8b0c9df16d. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6114.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: d column: 18 CC High quality sequence stop: 573 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..617 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6114" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 617 BP; 203 A; 145 C; 93 G; 176 T; 0 other; gaaacaacag atcaattaac tatgcacaca aatatagaca aattatcagt tcacgtcaaa 60 gaaacaaagt caagattaaa aagaaaacat tatacaatta tctgatttta tgaatggacg 120 tagaacatat atatcttcat atccaaaccc aaaataaaag aagagaagac ataaaggact 180 acattgatgc agataggttc tgataggttc tcttcaactg cagcaacctc cagccgaagc 240 agaagggcca tccctacctc cagaaggtcc aggaattcca ccatatccta cttttattcc 300 ataagactca tttgatacat caaaaactcc atcttgaatc tttttgtata tggttgcagc 360 tgtttttatg aatgcctctt caacattctg agcagttttt gctgaggcct ccatgaagat 420 caatccatgc tcctttgcaa attgctcgcc ttcctctgtg cttacagccc ttctatgagc 480 aagatcacac ttgttgccaa tcagcataat agtcatattt gcatttgcat gctgtcttgc 540 atcttccaac cagctagcca aatgattaaa tgtctccctc ctggttatat cataaacaag 600 caatgcacct gcagccc 617 // ID EH224939; SV 1; linear; mRNA; EST; PLN; 509 BP. XX AC EH224939; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5655.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5655 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-509 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 41ad537bf753ae69385dcf27d1bded87. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5655.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: m column: 22 CC High quality sequence stop: 404 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..509 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5655" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 509 BP; 175 A; 113 C; 80 G; 141 T; 0 other; aagagataat gtactagtga ctcaaatata tgtataatat aggaaatatt ctgttacaaa 60 accaacaaac acatgaaaga tacatcagaa aatttttaaa acctacgcac tcctacaaaa 120 agcatgaagt ttggtacatc caagtgcctt taatttacta cacaaatact atttagttga 180 aaggccaaga atgatcaata cgtcactatg gctattaaaa tccaacatga acctactcaa 240 tgtcgaactt ctttcttatg tccataatga actcatagac cttgtgttga tcaggaagag 300 acttggcaac actgtctttc tgcaggcacc tcttggccca agcaacaaac ttggggcact 360 cactctctat gttgaggctg ccaaaagtct tgaaccaagt gtcgaatgga ataagtgcta 420 tatccacaaa acctagatcg tctcctccaa aataagtctt tgtctcccag ctgttcctcc 480 aataaattta agggcgctct atgaactcc 509 // ID EH224940; SV 1; linear; mRNA; EST; PLN; 218 BP. XX AC EH224940; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6117.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6117 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-218 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; c4cd5222c3853c79442c0900859bfcb8. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6117.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: j column: 18 CC High quality sequence stop: 218. XX FH Key Location/Qualifiers FH FT source 1..218 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6117" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 218 BP; 65 A; 31 C; 45 G; 77 T; 0 other; caattgcata tcatgaatgt ttttgataat catatgcttg ccaaatagta tatgcatatc 60 atgaatgttt ttgataatca tatgcttgcc agatagtata tgcattattg gatactgtac 120 gagaaaactt gagtgtgggt tgaaggttga acagtccctt gctgtgacac aagcaggagc 180 attattatgt caaagatgca cttgtgattt ttattcgc 218 // ID EH224941; SV 1; linear; mRNA; EST; PLN; 647 BP. XX AC EH224941; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5525.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5525 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-647 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 2222d3fdd0873b1485d2b491032757a1. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5525.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: j column: 13 CC High quality sequence stop: 535 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..647 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5525" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 647 BP; 172 A; 134 C; 120 G; 221 T; 0 other; tttttttttt ttaaagtcaa cacacaattc attgaaaggg gtcatagata tacagttaac 60 aatgctttgc agaacagaat atgatgtgtt gaactaccaa attaagttcc tacgtaggcg 120 aacaaactcc tcaaaggatt ctggtgcctc atttgccaga atatatagga tattaataga 180 gtatgtcatc cttgtgacta gcatgcacga gaacaaaacg aagcttttgc cattggtaga 240 tttttggttt aggatcctgt cagaactgca ttgcctagtt gcgccattta tggctgatta 300 tttgatgctt gttcatgtat tctattaggc ttcatgttca aatcaatcat tttactttcc 360 atcttggcat gcccatcagc atcaggtcct ggcgttggaa ccataggcat cttgtgattt 420 tgttgcttct ctcccatttt tagtttggga agtggatttt gtcctctttt agcctcacct 480 ggttccaatg gttgtgaagc cggattttca gcatgactag atttatctcc tggaaactca 540 actctttgcc tgaacagaag atatcttcca tgcgttcttt tacctccaac atgaacaatc 600 atatcacact gcaaaccaat gggaacttcc atctgtctcg caataga 647 // ID EH224942; SV 1; linear; mRNA; EST; PLN; 281 BP. XX AC EH224942; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5501.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5501 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-281 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 249ecb2493015734d0c6fd843fe63e9d. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5501.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: j column: 7 CC High quality sequence stop: 258 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..281 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5501" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 281 BP; 103 A; 42 C; 31 G; 105 T; 0 other; attaatgcta cacctaatta atagctacct tcaaactacc tttctcatat cgtattacct 60 tttttttctc ctgtccttcc acaatgcaaa taagacagat cgaggaataa ctgaaaaaca 120 ataaagggcg taaaaatgtt ttctttaaat gatatgatta tatataataa tatgataaag 180 catcttccta tatatattat tatttggatt attatgtgaa gtagtactgt atatatatat 240 atatatatat tgaagtttaa atggctccaa attctctaac a 281 // ID EH224943; SV 1; linear; mRNA; EST; PLN; 705 BP. XX AC EH224943; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5509.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5509 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-705 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; d0450ddd591fe56586e986037b944201. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5509.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: j column: 9 CC High quality sequence stop: 683. XX FH Key Location/Qualifiers FH FT source 1..705 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5509" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 705 BP; 179 A; 150 C; 180 G; 196 T; 0 other; agcatctaag tgagatacac agtagttagg tagcaagcat aagtgtctaa ccttagaaac 60 atatctgcta catatcgtgt gcattttgga attgtgccac taaaattcta ttcatctatg 120 gctgcctcat cttatgctat gcaatcaatc cttgcaaacc ctatgatccg catttccagc 180 gggtctaggg agaaccattt tggtgttcct gcttatcaca tgagaaggaa tgttggcctg 240 agagttaggt ccatggctga ggaagagcaa ccaagtcagc ctgcgacccc agttacacca 300 ccaccagcag aacccaaacc acagccagtc tctactcctt caccaaaggt gagcaccaag 360 ttttctgatg tgttggcgtt cagtgggcca gcacctgaga ggatcaatgg aaggttggcc 420 atgattgggt tagtgggtgc aatggcagtg gaagtagcca aagggcaagg tgtgtttgaa 480 caaatatcca atggtggtat cccatggttc ttgggaacaa gtgtggtcct tacccttgct 540 tccttgattc cactcttcca agggatcagc gtggagtcta agtccaaagg gttcatgtcc 600 tcagatgcag aactgtggaa tgggagattt gctatgttgg gtttggttgc tctggctttt 660 actgagtatg ttaagggtgg taccttggtg taattcagtc atgaa 705 // ID EH224944; SV 1; linear; mRNA; EST; PLN; 372 BP. XX AC EH224944; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5506.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5506 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-372 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 83db8f275606ba5ae8f3fdc3bcd86888. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: d column: 9 CC High quality sequence stop: 239. XX FH Key Location/Qualifiers FH FT source 1..372 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5506" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 372 BP; 109 A; 71 C; 95 G; 96 T; 1 other; acaagctagc tagtaggcat gtgagggttg gtcttgctag tccaagtgat gtaccacggt 60 gtgacatatg tgagaatgca cctgctttct tctattgcga gacagatgga agttcccttt 120 gtttgcagtg tgatatgatt gttcatgttg gaggtaaaag aacgcatgga agatatcttc 180 tgttcaggca aagagttgag tttccaggag ataaatctag tcatgctgaa aatccggctt 240 cacaaccatt ggaaccaggt gaggctaaaa gaggaccaaa tccacttncc aaattaaaaa 300 tgggagagaa gcaacaaaat caccagatgc ctatggttcc caacgccagg acctgatgct 360 tgatgggctt gc 372 // ID EH224945; SV 1; linear; mRNA; EST; PLN; 438 BP. XX AC EH224945; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5827.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5827 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-438 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 633015cf844ce846afeebf82a4220825. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5827.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: e column: 17 CC High quality sequence stop: 186 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..438 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5827" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 438 BP; 152 A; 107 C; 76 G; 103 T; 0 other; aagccggaaa tttttaactg cccagcccac agaaaaaaaa ttttgtatga tggaaaaaaa 60 aagccaacgg ctaccccatg acaaaaatta ctactggcaa actaaataaa cgacactaac 120 cgaaatcaaa tatcattccc aaaaatcaaa aaggggggaa aacaaatcaa atggcttcac 180 ttatcaagga aggaaagggg gaggcggtgg caggttttat tttgcaaatc ccacccatgc 240 aaaaatgccg actgccaaaa ttatccctcc aaatatgtca tacttcaaaa cactgccctt 300 ctttttcttc tgagtctttg tacccgaagc aaaagtaaaa aatcccgctg ttgctgcccc 360 ctgctgctgc tgaccaaatc cctggttatg gatttctaca tgcagctcag gagccttagc 420 agccacttcc aatgattt 438 // ID EH224946; SV 1; linear; mRNA; EST; PLN; 479 BP. XX AC EH224946; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5668.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5668 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-479 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 2a88209d9189db2d8a437d7ab2948b8b. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: h column: 2 CC High quality sequence stop: 468 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..479 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5668" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 479 BP; 131 A; 119 C; 123 G; 105 T; 1 other; aanaaggaac aacaataatt atgaaagcct agagccaatt aagattgatt tttctctaac 60 tagcccaaag catatgttta atcttaatca cttgcagcca ccagcaccaa acccaattct 120 tccaccacca acatcataca caacctctat tgttttctgc tgcacgttcc catatatagt 180 aacatcacta tcgtcaccat ttgcagcaaa agccaagcaa acttgcttgg cactggccac 240 gtagagtatc ccttgcggcg gaagctgcac cgtgacgccg ccggcgaagg aaaaatctat 300 cttgggaata gaaaagacct cgtagccgct gagatcataa cacgtgtcga gtatggagag 360 ttccccggcg gaggggtatt tggacatgcc ttgcctgaag gcggagcgga gggcggtgta 420 ggcggtgggg gggaggcggg tgatcacggt gccggagtcg atgattgcac cgccggtgg 479 // ID EH224947; SV 1; linear; mRNA; EST; PLN; 643 BP. XX AC EH224947; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5978.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5978 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-643 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 7dc2e857af5f4fe7c3717060e98a3e08. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5978.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: c column: 8 CC High quality sequence stop: 596. XX FH Key Location/Qualifiers FH FT source 1..643 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5978" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 643 BP; 177 A; 167 C; 142 G; 156 T; 1 other; caattttcgc tctttcggtc tctccaaatt gagttcagag tcaccaccga atccgttcct 60 acaatggcga cggtgacgtt ttccttttcc gctaaaacct taactctacc ctatcacccc 120 aaaacctcaa tctcgagctt cgctccggta acgaatcttc gcctctctca ttctatcgtc 180 acccgaaccc gaaattgtaa ccggattcgg gcggcgctgg atggggattt ctcggcgaaa 240 aggagcaaca acaacgagca gagggagacg ataatgttgc ccggctgtga ctacaaccat 300 tggctgattg tgatggagtt tccaaaggac cctgcaccca ctcgtgaaca aatgattgac 360 acttacctcg acactcttgc cactgtcttg ggaagcatgg aggaagctaa gaagaacatg 420 tatgccttta gcacaaccac ttacactgga tttcagtgca ctgttgatga agcaacctcc 480 gagaaattca agggtttgcc tggggttctt tgggtactgc cagactccta tattgatgtt 540 aagaacaaag actacggagg tgacaaatac ataaacgggg agattattcc ttgcaagtac 600 nctacctacc aaccgaaacg tagcgcaccg aagaatgaga gta 643 // ID EH224948; SV 1; linear; mRNA; EST; PLN; 797 BP. XX AC EH224948; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5662.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5662 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-797 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 60d14801e1757dd62e601cd0357fcbad. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: k column: 24 CC High quality sequence stop: 702. XX FH Key Location/Qualifiers FH FT source 1..797 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5662" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 797 BP; 257 A; 139 C; 178 G; 223 T; 0 other; agagtgaatc cttcagatgc ccgacaccac agaggtagtt ttggagaagc actggctaat 60 catgaaaaga agtcgaactc taataacatg gagaagcttg agacgtggaa gatggctctg 120 caccaagtgt ctaacatttc tggccatcat ttccaacatg atgggaacaa atatgaatac 180 aagtttatta aggagatagt tgaatcggtg tcaaacaagt ttaatcatga tcatttacat 240 gtttcagatg tcctggttgg gctagagtca ccagtgctag aagtaaagtc gcttctggat 300 gttggacgtg atgatgtcgt ccacatggta gggatccatg gactcgccgg agtgggtaaa 360 acaacacttg ctatcgcggt ctataattct attgctggcc attttgaagc ttcttgcttt 420 cttgaaaatg tgaaaagaac ttccaacacg ataaatgggt tagaaaaact ccaaagcttc 480 cttctttcta aaaccgctgg agaaattaag ttaacaaatt ggagagaagg aattcccatt 540 ataaagcgta agctcaagca aaaaaaggtt cttcttattc tagatgatgt tgatgaagat 600 aaacagttac aagcacttat tggcagccct gattggtttg gtcttggcag tagaatcatc 660 ataaccactc gagacgaaca tttgttagct ctacacaatg ttaaaataac atataacgtg 720 agagagttga atgagaaaca tggtcttcaa ttacttactc agaaggtttt gagttgggaa 780 acggattgat ccagtta 797 // ID EH224949; SV 1; linear; mRNA; EST; PLN; 730 BP. XX AC EH224949; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5513.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5513 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-730 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 5261abb0b7cbd39bdcfd27bf7e0d12a7. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: b column: 11 CC High quality sequence stop: 676. XX FH Key Location/Qualifiers FH FT source 1..730 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5513" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 730 BP; 164 A; 157 C; 165 G; 244 T; 0 other; ctttggtgca atactaatgt gcttcttgcg caaacggagt gctaatagca agggacaaca 60 agagctttca ggtgcagatg ctggcgcatg tgcgtccttg aagtctctat ccaaatcact 120 tgccagtgca ttgtctgatg taaagatgtt gttgatcata ccccttattg catattcagg 180 tctacaacaa gcatttgtgt gggctgaatt cacaaagtat gttgtaactc ctgcaattgg 240 tatttctggt gtgggtagtt caatggcagc gtacggggct tttgatggaa tatgttctct 300 gcttgctggt cgtctcacct ctggtctcac atctattaca acaatagttt ctgtaggact 360 tttcgctcag gcagtcgtat tagtcttact tctgctaaat ttcagcataa gtagcggatt 420 tcttggtact gtttacatac tcttcttggc tggtttattg ggcatcggtg atggagttct 480 aatgacacaa cttaatgcac tgatcggaat actatttaag catgacacgg aaggggcttt 540 tgctcagcta aagatatggc aatgcgctac aattgctatc gtgttctttt tcgccccgct 600 tatctcgttc aaagccgtgc ttgtgattat gcttgctctc ctttgcttct cattctgcat 660 tttcctattg cttgctctga aggtggggaa ggcaccatca ccctccacca gtcagcgaaa 720 ttaaagctgt 730 // ID EH224950; SV 1; linear; mRNA; EST; PLN; 622 BP. XX AC EH224950; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5509.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5509 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-622 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; bbd2614720bb352ebce649678cd164eb. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5509.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: j column: 9 CC High quality sequence stop: 523 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..622 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5509" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 622 BP; 182 A; 163 C; 124 G; 153 T; 0 other; aaaaaaaaat gataatttta tatgctgatt aaataaaggc accaagtaca aaaagagatt 60 ggtctttcta accacgaatg acttaagacc attttggtgt gcattcatga ctgaattaca 120 ccaaggtacc acccttaaca tactcagtaa aagccagagc aaccaaaccc aacatagcaa 180 atctcccatt ccacagttct gcatctgagg acatgaaccc tttggactta gactccacgc 240 tgatcccttg gaagagtgga atcaaggaag caagggtaag gaccacactt gttcccaaga 300 accatgggat accaccattg gatatttgtt caaacacacc ttgccctttg gctacttcca 360 ctgccattgc acccacaaac ccaatcatgg ccaaccttcc attgatcctc tcaggtgctg 420 gcccactgaa cgccaacaca tcagaaaact tggtgctcac ctttggtgaa ggagtacaga 480 ctggctgtgg ttcgggttct gctggtggtg gtgtaactgg ggtcgcaggc tgacttggtt 540 gctcttcctc agccatggac ctaactctca ggccaacatt ccttctcatg tgataagcag 600 gaacaccaaa atggttctcc ct 622 // ID EH224951; SV 1; linear; mRNA; EST; PLN; 712 BP. XX AC EH224951; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5518.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5518 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-712 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ac8776c63f7d085d56bcf3f6c56ba812. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: l column: 11 CC High quality sequence stop: 674 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..712 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5518" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 712 BP; 219 A; 165 C; 164 G; 164 T; 0 other; aaagcagata aatatttata atattttatt taattagata cggaaaaggg agggaaatgc 60 aggattctta tttgctatac aatccgaaac tgaaactctt taaacctcta ttatacatga 120 gttagtaaaa agggtccgtt gagaaaaaat aaacagaacc taataggaaa gactactcga 180 agcataaaca gcttgattcc agcaaattaa gtgagaaaat tggatatagt tgcagtggtg 240 agagccccag tcaaaatcca cgggtgtcgc aaggcttgat gtgcggaaat gcggttagag 300 ggatccctgg aaatcatttt cctaaggagg tccttagcag gtgcagaaac agagctaaag 360 attaggctgg gaaacctaag attagccctc aaaacagact caaagatttc gggagcagac 420 tctccataaa agggtggaaa gccagccaac atggcgtaaa gaatgacccc gctgctccac 480 acatcaacct tctcatcgta ctctctcccc ataatcactt cgggtgcaac gtaatagggc 540 gtccccacca caccgctcat gctactcccc tcacccaacc actcggcgga cccgaagtct 600 gagagcttaa gcttgttccc ttcatcgaac aggatgtttt ccggcttgat gtccctgtgc 660 gcgagtccct gggcgtggca gtgcgccacg ggctccagaa gctgtttgag ga 712 // ID EH224952; SV 1; linear; mRNA; EST; PLN; 391 BP. XX AC EH224952; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5522.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5522 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-391 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; fb59bda30f53f6186f7dcb5d0b83a79f. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: d column: 13 CC High quality sequence stop: 391. XX FH Key Location/Qualifiers FH FT source 1..391 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5522" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 391 BP; 122 A; 64 C; 71 G; 134 T; 0 other; ttcaagccta tcccgaattc aatttgaacc acaagtattt ggaagggcca aaaagggata 60 taaaatgggc gtcaaggaag ctgacagaca acgggtttgt gtacaaaaat gacctgaaga 120 tgatattgga tgattgtatc agatgcgcaa gaaggatggg cgacctctag tttttttaat 180 tctgaaagct tgcatgcgga tcctctcaag tttggagtcc ttctacaatc tgcatccttt 240 cttttgcaaa ttccatccac tgcattaatg acatttacgg ttattaataa atttgtccct 300 tccaatatta ttttaaatgt atctattatt tactttgaca gtggagaaac agattttttt 360 tttaatcaat tgataaatta tgatatatat t 391 // ID EH224953; SV 1; linear; mRNA; EST; PLN; 684 BP. XX AC EH224953; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5532.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5532 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-684 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; de6e8f477807de45900aa12fd2862ce4. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: h column: 15 CC High quality sequence stop: 651. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5532" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 684 BP; 217 A; 131 C; 136 G; 200 T; 0 other; ttaatcctaa catgaattta tcaagtgcct taaatatgcc tctactaaaa accaatggag 60 gaaaaattct gcaacagaat cagagttcta gtagcagttc actacttgga ggaatcccaa 120 tatgtagtgg gagtaatctt ttgaggacaa atatgattaa ctgctcagtt tcaaatcctc 180 ccaaagtatc cacttctgat ttttctggaa cacacaaggt tgggtttggt cttcaaagta 240 acaatgcaac aactaatgct gggctttgtt ctgtgcctaa tttcactaat caatcagtta 300 ccagtcacat gaatctagaa ggctctgatc agaagaatca ggcatatgat gctttcgctt 360 cagctgatca aaggcaagat gatgatttgc ttcaggctgc tcttaagatt ccatctctta 420 atcttgagga gcatgtgcct atgggtgatc aaatccctgg ttttgttcaa gattgcctca 480 ataaagatgt taccagtcaa catatgatga aaatgaatgt taagcatgaa gaagcctatg 540 ctcaacttcc atctggtgat gacctcttcg atgttttagg tatggatttg aaaaggagac 600 tactcaatgg aagctgggaa taagttgctt gccacagatt cagatgacaa cacagaacat 660 ctagataaaa aggcacatgc catg 684 // ID EH224954; SV 1; linear; mRNA; EST; PLN; 578 BP. XX AC EH224954; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5523.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5523 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-578 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 0b9c54fe019c87e1489f2202aef9c428. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5523.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: f column: 13 CC High quality sequence stop: 578 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..578 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5523" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 578 BP; 150 A; 118 C; 143 G; 167 T; 0 other; agaagcatta gcactaggag cagcaacaac tagtctcagc tgaaatggcc tcttcagtga 60 tggcatctgt ggccctcaaa cctgcccctt tcactgttga gaagtcctca gtgagaggcc 120 ttccctctct ctcaaggaac tcttcttcat tcagagttgt ggccagtggc aagaagatca 180 agactgacaa accttatgga attaatggtg gcatggcttt gagggatgga actgatgcat 240 ctggcaggaa aggaaaggga aagggtgttt accagtttgt agacaaatac ggtgctaatg 300 tcgatggtta cagtcctatc tacgacacca aggactggtc tccaaccggt gatgtctacg 360 ctggcggtac gactggcttg gctatctggg ctgtgactct acttggtctt ttagctggtg 420 gtgcacttct tgtctacaac acaagtgctt tggttcaata gactttgcaa atcgatgttt 480 aagttataac atagtccatg taactatcta aaaatgaaga actattcgtg aagtactttg 540 tggatatgtt ccttctgttc tataaatgag tggatgtg 578 // ID EH224955; SV 1; linear; mRNA; EST; PLN; 577 BP. XX AC EH224955; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5523.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5523 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-577 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 7b32de4705c79499964a53f9b47b2dc0. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5523.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: f column: 13 CC High quality sequence stop: 577 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..577 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5523" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 577 BP; 167 A; 142 C; 118 G; 150 T; 0 other; acatccactc atttatagaa cagaaggaac atatccacaa agtacttcac gaatagttct 60 tcatttttag atagttacat ggactatgtt ataacttaaa catcgatttg caaagtctat 120 tgaaccaaag cacttgtgtt gtagacaaga agtgcaccac cagctaaaag accaagtaga 180 gtcacagccc agatagccaa gccagtcgta ccgccagcgt agacatcacc ggttggagac 240 cagtccttgg tgtcgtagat aggactgtaa ccatcgacat tagcaccgta tttgtctaca 300 aactggtaaa caccctttcc ctttcctttc ctgccagatg catcagttcc atccctcaaa 360 gccatgccac cattaattcc ataaggtttg tcagtcttga tcttcttgcc actggccaca 420 actctgaatg aagaagagtt ccttgagaga gagggaaggc ctctcactga ggacttctca 480 acagtgaaag gggcaggttt gagggccaca gatgccatca ctgaagaggc catttcagct 540 gagactagtt gttgctgctc ctagtgctaa tgcttct 577 // ID EH224956; SV 1; linear; mRNA; EST; PLN; 458 BP. XX AC EH224956; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6000.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6000 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-458 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3934e7e6edd31f19ae328f044bb51f87. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: o column: 12 CC High quality sequence stop: 436. XX FH Key Location/Qualifiers FH FT source 1..458 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6000" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 458 BP; 137 A; 102 C; 86 G; 133 T; 0 other; ttctatgtcg catatttttg aagaagagaa gcggtgctaa aaatggggag gagagcaaca 60 aggtgaggaa ctctaaggtg gttttctatg acttcctagc gcagaacaag actgattcct 120 catcctcggc cgccagtgga attacacatg aacatgaatc agatgaacat gaccatgaag 180 agagcagtag ctccaacacc ttcccttata ctattagaac gaaaccttaa caaccaagtc 240 aacaaccacc ttccttaaaa agttgattat cacctagttt tttttttttt aattctcttt 300 ccctttccct gtaatcatca tcaaccactt gttgaaagga agcatccctc ccaatgagac 360 cggcattagt taaagggtag cctgcagagt atggtactga tagtagcagt gtgtaatgga 420 ctccccattt tccttcaagt taaccttttt ttctaatg 458 // ID EH224957; SV 1; linear; mRNA; EST; PLN; 619 BP. XX AC EH224957; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5527.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5527 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-619 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3f1247579d6d977396590125b45bc760. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5527.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: n column: 13 CC High quality sequence stop: 581 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..619 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5527" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 619 BP; 199 A; 134 C; 84 G; 202 T; 0 other; acaccaaaca ttttaggtat gctgtattat ataatcagaa ttttgtaaat tgatggtgca 60 actgtactgc ttaataatta aaattagaaa acttctatct tatctaattg taacagacaa 120 ctttataaat gttgctgcct tgtattcaaa ttgtgtttaa aataaacaac ttattcattc 180 tctatatggt atcagtctaa gcttattcgt gaccttgaag gctacatcat tggcaaaaca 240 ccttgacctc ttttgatgag gtatttgtta aattgcagaa ctatgagatg tatctcaagc 300 gtgatgagca cttcaatcac cactcctcca tcagtgccaa ctttgccaat caccgctgct 360 cacaccactc tgccagaggg tgcaattagc attacccatc tggcaagcat cgtagctctt 420 catactccaa acatctttgc ccaagtttaa gatcagtttt tgctgcagta attcacattc 480 aattccttgg caatgaatcc ttttaatctc atataactta attccttaaa ttaaatcatg 540 agactaatgt acaaaagcac aacaaaatcc ctagatcaaa cacggttctc tatttctaca 600 taatcaaata gcatgaccc 619 // ID EH224958; SV 1; linear; mRNA; EST; PLN; 555 BP. XX AC EH224958; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5846.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5846 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-555 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 8626be227e8265b777d77eb8a22de8ad. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5846.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: k column: 21 CC High quality sequence stop: 551 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..555 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5846" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 555 BP; 163 A; 138 C; 129 G; 125 T; 0 other; aggcgaatta tagatgtcct aattttattg aaggtagctc atcatgtttt ttttatgctt 60 ctcataacag actccattca ctcactctat gaaccccaac caaccaacca catgatactg 120 atattgaaaa ggaaagggga agggtttcga aatgatatcc ttaacaccac aatcccctaa 180 acgacgacac gagaaagaaa agaagagaaa aaaaaggcaa aaacgacacc gaaagaaata 240 acacagattc agagattaaa gccgcgggtc aacgtcgtct aagagagttg accgcagttg 300 gcgatgacaa cgggcttgga ggtcctgccg gagctggatc cgaccttctc gatgtccttg 360 acaacgttca atccttcgat gacctgtccg aacacgacat gctttccgtc gagccactct 420 gtcttctccg tgcaaatgaa gaactgagat ccgttggttc cgggaccggc gttcgccatg 480 gagaggatgc cgggaccggt gtgcttcttc acgaagttct cgtcggcgaa cttggcgcca 540 taaatcgact cgccg 555 // ID EH224959; SV 1; linear; mRNA; EST; PLN; 456 BP. XX AC EH224959; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5675.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5675 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-456 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; c93a7873bd30315af7afb27b7140c318. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: f column: 4 CC High quality sequence stop: 432. XX FH Key Location/Qualifiers FH FT source 1..456 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5675" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 456 BP; 115 A; 81 C; 95 G; 165 T; 0 other; ggcaattttc tgatttctca cacccaaaca ttgcctgcaa ttactccacc gggaccctac 60 tctctgaaga tgacattgaa ggatgatcgg gaggaggtgt taacttgcgt taagtttaat 120 tttaaaattg tttttggatc ttttgtgtct gatatgtaaa gtgtcctttg catgcctgat 180 ctggggaact tttttgcttt gttgtgaagt gttttattgt attttgcact gtatatcacc 240 tttcaattca agagtcgtat cgtataaggc caatattctc taatgtcaga gtcattagac 300 tcacgatgtt gtataaggtc catgactgaa tttctctttc tgtatttgca tagtgcaatc 360 catagttctg aattgcggtc gcggctgcta tgatgcagat tgcggcatga aattattata 420 tgattgcacc gaatgccaaa aagagttcta cagtta 456 // ID EH224960; SV 1; linear; mRNA; EST; PLN; 584 BP. XX AC EH224960; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5533.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5533 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-584 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 77bcd4f1af959a37ac03edd31411cc99. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: j column: 15 CC High quality sequence stop: 501 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..584 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5533" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 584 BP; 179 A; 153 C; 76 G; 176 T; 0 other; tttgttaatc tgcagagctt gcaattaaaa ttcaaccttc tatacaaata ttatcacaac 60 cttcaaaaga ggatcaaaca agtctaaata aagaagttga atttaacaag cattgtgtaa 120 agcacctacc acaaatcagc cagtacaaaa tcaaatatga agccagatgc tttcttttgt 180 gaagatcacc attgttctct tggacgacca tcagggagta caggaagaag gaccatttca 240 tcattctgtc tcgagtcttg tttaatagtc tttccttgcc ccatctccaa aagttcagca 300 tcctcatcaa aatctggcgg caaatcatca tcttcttcat cactccagtc atcaccatca 360 tcaatgtcat caaacccatc atcattcatt aaattatcaa catcttctaa ccattcttct 420 tcctcttcac cactttcatc ttctctgcta tcacttcctc tcatgctatc ttcaactatc 480 ctgcgagcag gtcccttggg aaaccgtgga acattaacca gagtacgaag cttttccttt 540 ataagcaaca accggtcctt ctccaccaac tgagaatctt ggta 584 // ID EH224961; SV 1; linear; mRNA; EST; PLN; 756 BP. XX AC EH224961; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5527.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5527 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-756 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 7f5667eea12474c9a560a7637b99e02f. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5527.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: n column: 13 CC High quality sequence stop: 644. XX FH Key Location/Qualifiers FH FT source 1..756 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5527" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 756 BP; 201 A; 139 C; 161 G; 255 T; 0 other; agtggattca tttcatcaat cactttgcct ttctgatcat aatcaccaaa atgccaacca 60 tgaatccagt tcgtttcaaa tacatgcaag ccataataat atttatgatg ttgcaggttg 120 ttgtttctgc tcaagaccat attatgtgca ttgagactga gaaggaagca ctcctccaat 180 tcaaggctgc acttctggat ccctatggca tgctctcttc ttggaccact tctgattgct 240 gccaatggca agggattcgc tgcaccaacc tcaccgccca tgttctaatg ctcgaccttc 300 acggtttgaa tcgttcttgg agacatgctt atttcaagtt cataagcaat ttctcagatg 360 ctatttatgt catggcagcc gtgaaagttt tcaagttgca tcacagagga tagctgaaaa 420 attctgaatt gtaggtgcca gcttgttgag gtggaaaatg caaagagaca cttttaattt 480 ggaggtgaga aggcatctct cacagttgga tttggagacc gagagagaat taaggaaggc 540 ttctagagct ttagggagcc aattgtgatt gtgatccatc tagtttattt tcctttttaa 600 tttctaaaca ttttgtttgt agtttggcat gtaaatctga actggtttgt tttgtatgaa 660 gttgatgaat cttcataata atgggaactg agaggtttgg ttattgcttc ttccattttt 720 gggtcatgct atttgattat gtagaactag agaacc 756 // ID EH224962; SV 1; linear; mRNA; EST; PLN; 684 BP. XX AC EH224962; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5684.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5684 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-684 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; af4503f9f7a2618224f8d9f3d329fd61. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: h column: 6 CC High quality sequence stop: 616 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5684" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 684 BP; 233 A; 151 C; 113 G; 187 T; 0 other; ggaacaagca gaaaatgtat attcttattt aattaccagt cattggaagg tacaaactct 60 tgtacaagag aattaaaaaa tcaaattccc attgtgaatc cccacatcaa ggatcattta 120 tatgtattga acaaattatc tttttctatc tggactacaa tccgggtaca tcaagtactg 180 tctccgctct atttttcttt actaactagc acagagcagc atcccaaatt acatgacttc 240 tccagaaaaa aatatattaa acattacaat tacttattaa ccaaaatcgg ggtatgaata 300 tgtggtaatt aagtcaaact agaaccaagc tgatcagcaa caaataaata acttcctaca 360 ataatgaatg atgccagccc agagcgagcc attcgccaac caataccacg gaacaagaca 420 cctctgtcag aaggatggat gccagtaatt ctttcaaact tggttcctgg tcgtttccac 480 ttcagaatct ttctctccat ggagatgtac ttcggcagca cagtacattg tgatctactt 540 cttgtagtat caaaactatg tgaagcagta gcagcaactg aagctgagaa tccagcagta 600 agactaacaa tcaaaggaga ccatggaccg acttcttcat ttaacctggg tggaggatcc 660 atccctacag ccttccaatc tagg 684 // ID EH224963; SV 1; linear; mRNA; EST; PLN; 459 BP. XX AC EH224963; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5530.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5530 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-459 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 9a68e52bb6084aaeaf7e5f8098c7a3ee. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5530.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: d column: 15 CC High quality sequence stop: 428 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..459 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5530" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 459 BP; 168 A; 95 C; 67 G; 129 T; 0 other; atataggata ctagtattac aattgcgaaa gccgacggaa aaatataaat aaaacaaact 60 atgagaggca aactgaatta ttggtaaatg tgaaaaaaca aaaaggttgt tttttgtatc 120 tagaaaaaag tttgaataca ctaaaaaatg cctcatttta ccacattttc catcccttta 180 aataacttat ttcatataac caaaccccat tgaagcacaa acatcacaag atgagcttat 240 ctcatcctgt gtttcacata cgatgtacca agatttgaaa tttcagttgg catttgaagc 300 atttttcaaa acccattcca ctaaagtagc aatgccaaat cctgttgctg agcgcttctt 360 gtcattccac acaggaccag ccatgtcaat gtgcatccat tgaacctttt catcaacaaa 420 ctgtttcagg aagagagcag caacaatagc accaccttg 459 // ID EH224964; SV 1; linear; mRNA; EST; PLN; 528 BP. XX AC EH224964; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5716.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5716 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-528 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; c1c8ae3435ded8186160040b64dc2c3b. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5716.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: h column: 14 CC High quality sequence stop: 528 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..528 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5716" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 528 BP; 167 A; 128 C; 80 G; 153 T; 0 other; agagaactca agggtatcaa catttcaaat ctgacaatta cataataaag cctttcaata 60 ccttgataaa ttccatgtca ttgccaaagc accaagaaaa atcaatcatg tttcagtgca 120 cacattcaga aacataaaga gaaacttcac gagtttgggt atttttatct ttacaaatat 180 ttgaccagca gccatatgac aacaaccaaa cccactatac caatgactga taaaagcatg 240 cacaagcagg agcaaccacc cttggtgcgt ttgtttaaca ctgccaagtt cttctgcact 300 ctcctcagcc gagaatctgt aacatctaca tgttggtcca agtcatcaat aagtctggta 360 tgtagattca gctcttcatt cacagccaac gcaatatgtt tagtactgat tacagtctcc 420 tccaatttct caaggccatc gtcttgctct ttcataattt gccgttgaag accaactagt 480 ccactgttgt acaggccaac agttctgctc attgcatctg atttcatt 528 // ID EH224965; SV 1; linear; mRNA; EST; PLN; 661 BP. XX AC EH224965; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5530.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5530 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-661 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 43004bdf29c985dcd6283aa0e5ba874b. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5530.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: d column: 15 CC High quality sequence stop: 568. XX FH Key Location/Qualifiers FH FT source 1..661 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5530" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 661 BP; 169 A; 136 C; 162 G; 194 T; 0 other; gatcaaaaag ggttggattg attggtcttg gtcagtcacc ttccactcct gcacctttta 60 agagtcttgg cgaggccatt gtggcggctg ccaagtcttc tcaagcaagc agtgttgctg 120 ttgctcttgc ctcttctgaa ggactctccg cacaatcaaa gcttagcact gcttatgcaa 180 ttgcttctgg ggctgtgctg ggattatttg aagataatag atacaaatca gaagcaaaga 240 aatcaacact aaggtccatt gactttattg gtcttgggac aggacctgaa ctggagaaga 300 aactgaagta cgcaggagat gtttcttccg gaattatctt tggaagggag cttgtaaatt 360 ctcctgctaa tgtgctcaca ccaggggtgt tggcagaaga ggcagctaag attgcttcca 420 cctacagtga tgttttcact gctaaaatat taaattctga gcaatgtgca gaattgaaaa 480 tggggtccta tctgggtgtt gctgcagcct cggctaatcc tcctcatttt atccatctat 540 gttacaaacc tctgagtgga cctgtcaatg tcaagttggc attagttgga acaggtttga 600 cttttgacag tgggtggcta caacatcaag actggacctg gctgttcaat tgagctcatg 660 a 661 // ID EH224966; SV 1; linear; mRNA; EST; PLN; 524 BP. XX AC EH224966; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6010.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6010 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-524 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 6d2f404ecad31f8cfa594d3c02071f66. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6010.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: c column: 16 CC High quality sequence stop: 520 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..524 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6010" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 524 BP; 145 A; 121 C; 94 G; 164 T; 0 other; gtctttcaaa aaaaaaaaaa aaaaaaaagg taattttata acaaaaagaa aaaaaaaagt 60 tatattttta agatagaagc ttttgagctt cctccatttc tccatcatca tccaacacat 120 ttgccaagtc atcagtctct ccatttgcca agaactgatc aacctcagcc atcaggtcct 180 cctcctcctt tgatgaacta gtgagctttt tcacagtagt ggtctgagag accttttcag 240 attgattttg cttgttcagc tcagaactct ctggaatctc accctcatcc tcatcttcgt 300 catactcctc ctcgtgctcc actggattat cgattgcaaa ctgtggccta tccacaagca 360 ttgattgggc acgttgaatc aagggcggag gttgaggcat cgtctggcgc ttgtaattca 420 ctgtttctga tgccttgttt agatctaaaa tcttctcctc tgacatcgat tgattatgac 480 gaagattcat atctgctgga ggggatctca ttgcggcatg tgac 524 // ID EH224967; SV 1; linear; mRNA; EST; PLN; 602 BP. XX AC EH224967; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5719.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5719 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-602 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 46118def6c7ad08996efd4637ea33495. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: n column: 14 CC High quality sequence stop: 565 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..602 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5719" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 602 BP; 159 A; 135 C; 115 G; 193 T; 0 other; agctacgtga cagatcaatc aaactcgtta ttcatctcta tgataccctg ctgcaaaaga 60 gaaggcaatg ttgaactcat acgttttgaa cgttcactat gatttatgga acatgatata 120 aattgatttt cctccatgca tgtatgtgta taactattac actactagcc catagtatag 180 gaagggctgg gtaagttgat cacagtcgtt ttgaccttgg tcatcatgtt tatgctgttg 240 gtaatccctt gagatccaat gccactgtcc tttataccct gaaagggaaa atgatctggc 300 ccacgagccg gggcggaatt tatctgaact gttcccgttt ccattgcatc actgatcatt 360 attgctttgt tgacatcctt tgtgaagaca catccctgaa gtccaaagtt gctagcattg 420 caatgatgga ttccttcttc aactgaattg atcctaatta ctggtaaaac tggtccaaat 480 ggctcttccc atgcgattct catgtctggc ctaacattgt ccagtagcaa aggccaaata 540 agattgcctt ctcttttgta ctcctggcag aatgttgctc ctttctcctt agcatccaag 600 aa 602 // ID EH224968; SV 1; linear; mRNA; EST; PLN; 571 BP. XX AC EH224968; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5849.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5849 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-571 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 2a1845f729a1151e81b873d995008d61. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: a column: 23 CC High quality sequence stop: 570. XX FH Key Location/Qualifiers FH FT source 1..571 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5849" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 571 BP; 153 A; 164 C; 127 G; 127 T; 0 other; caagccccac tcaaccacca cacctctcct cgttcacgct acccctttct gctcttcttc 60 tacctttcaa gttttaaaag tataaagatg gcagagacat tcctatttac ctcagagtcg 120 gtgaacgagg gacaccctga caagctctgc gaccaaatct ccgatgctgt cctcgacgct 180 tgcctcgagc aggacccaga cagcaaagtt gcctgcgaaa catgcaccaa aaccaacttg 240 gtcatggtct tcggagaaat cacgaccaag gccaacgttg actacgagaa gatagtgcgt 300 gacacctgca ggaacatcgg cttcgtctca aatgatgtgg gactggatgc cgacaactgc 360 aaggtcctcg tcaacattga gcagcagagc cctgatattg ctcagggtgt acacggccac 420 cttaccaaaa aacctgaaga aattggtgct ggtgaccagg gtcacatgtt tggctatgcc 480 actgatgaaa cccctgaatt gatgccattg agccatgttc ttgcaacaaa actcggtgct 540 cgtctcaccg aggttcgcaa gaacggtacc t 571 // ID EH224969; SV 1; linear; mRNA; EST; PLN; 499 BP. XX AC EH224969; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5694.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5694 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-499 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 0a1e561e5d6704ba50605cc22481c3fb. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5694.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: l column: 8 CC High quality sequence stop: 451. XX FH Key Location/Qualifiers FH FT source 1..499 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5694" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 499 BP; 134 A; 116 C; 116 G; 133 T; 0 other; tcagaattct ctaaaagaga tctttttctg ctctttgaag aaagaagggt ctttgcttga 60 ttttggagat gtctctgctc tcagatctca tcaaccttaa cctctccgat accaccgaga 120 aggtgatcgc agagtacata tggatcggtg gatcaggaat ggacctgagg agcaaagcaa 180 ggactctccc aggaccagtt agcgaccctt cagagcttcc caagtggaac tatgatggtt 240 ccagcacagg tcaagctcct ggtgaagaca gtgaagtgat tttataccca caagccattt 300 tcagggatcc attcagaagg ggtaacaata tcttggttat ctgtgatgcc tacactcctg 360 ctggagaacc tattcccact aacaagaggc acgctgctgc caaggttttc agccatcctg 420 atgttgttgc tgaagtgcca tggtacggta ttgaacaaga atacaccttg tttgccgaaa 480 gatatccaat ggcctcttg 499 // ID EH224970; SV 1; linear; mRNA; EST; PLN; 619 BP. XX AC EH224970; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5853.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5853 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-619 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; d70fce1dfaabeb7bad827b27a992c769. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: i column: 23 CC High quality sequence stop: 614. XX FH Key Location/Qualifiers FH FT source 1..619 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5853" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 619 BP; 119 A; 221 C; 146 G; 133 T; 0 other; aatgttcata cctcaccaat ggaactctct tcttccttca cacactccca caacttgttc 60 tctttctcat catccctcac tcccactctt cgacgtcaaa ccctccgcgt ctccgcagta 120 accatagacc caccgcaaga gcttcccaag aacagccccc agcgtctcct taaggagctc 180 gccgagcgca agcgcccctc cccgcgaacc ccacgcgctc ccccgcgaag attcatcctc 240 acgcgcccgc tagacgacaa aagagtcgcg gacaggttcc tcaacagccc ccagctggcg 300 ctcaacacgt ttcccttgct aagctcctgc ctccccttct cccctctgga tcacctggac 360 aagtcatgga tggagaatca cttgatggaa gccaagcagg cgctggggta ccctctggag 420 ccctccctca ccctcgggga cgatagcggc cccgccatgg agctggacac gctgctctac 480 ttggcgtttc agcatgaatc gtgtgagcgg agccgggcgc ggcacgtgcg cttcggccac 540 tccaggctgt tttttcttgg ccagtatgtg ctggagctgg ctttggcgga gtattttttg 600 cagaggtatc cgagggagt 619 // ID EH224971; SV 1; linear; mRNA; EST; PLN; 552 BP. XX AC EH224971; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6006.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6006 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-552 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 8a2f645cdb87ff455381fe0a3a6bb870. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: k column: 14 CC High quality sequence stop: 552 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..552 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6006" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 552 BP; 184 A; 108 C; 74 G; 186 T; 0 other; atgaagaaac agttatccaa gatgttggat cactggcaga aaaaatctca ttacataacg 60 atttagagca tcccttatgt ttatgtttac aagatttcaa attcattctg cttggttctt 120 tacagaatcc attgatgtat attaccaaaa tttaattcaa ccagagttta gtttaattta 180 tatacaccct ataaaacagt cttacacaaa catccaataa caatttactt tatcaccata 240 ttatatttat gaattcgatg acgtgataaa ataatcaaat cttattggat atctgtgtaa 300 aaaatgcttt acattgataa tatgtataaa ttaaactcat cagagaaact ttgattcatc 360 tgattgctag agaaatcaat gctttccatt tacttctatt gcaggacttg tcttcctcct 420 cctctcgcga aagcttcggc atgaagacaa gcttggaatg caccactgca aagactttac 480 taatttctct ctaatggatg cacgatcctc actcatggtc ctttcactac tacaagcaga 540 gttagcctct ga 552 // ID EH224972; SV 1; linear; mRNA; EST; PLN; 298 BP. XX AC EH224972; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5553.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5553 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-298 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 723ca34cd722fbe173005da79dd96576. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: b column: 21 CC High quality sequence stop: 294 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..298 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5553" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 298 BP; 84 A; 72 C; 49 G; 93 T; 0 other; tgtattcaaa ttgtgtttaa aataaacaac ttattcattc tctatatggt atcagtctaa 60 gcttattcgt gaccttgaag gctacatcat tggcaaaaca ccttgacctc ttttgatgag 120 gtatttgtta aattgcagaa ctatgagatg tatctcaagc gtgatgagca cttcaatcac 180 cactcctcca tcagtgccaa ctttgccaat caccggtggt cacaccactc tgccagaggg 240 tgcaattagc attacccatc tggcaagcat cgtagctctt catactccaa acatcttt 298 // ID EH224973; SV 1; linear; mRNA; EST; PLN; 604 BP. XX AC EH224973; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5539.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5539 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-604 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; d2252069d9b6f9c579e557ade47afd4e. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: f column: 17 CC High quality sequence stop: 602. XX FH Key Location/Qualifiers FH FT source 1..604 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5539" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 604 BP; 129 A; 174 C; 147 G; 154 T; 0 other; agcgccgccg ttgccctacc atcttcatat tattctattg cctttctttc acgtttttct 60 ggtttttaag cccttccagt gccctattta cgcttatacc cttctggtta tgatagcttc 120 cattcggcgt cagccagaga gccgaagctc ttggcttcca cacatacttt cggtgccgtc 180 gattgagata caaagcctcc aagccagccg agccaatcct caaattccaa ctttacccct 240 ccctgcaaat attgttttct tttaacggtt acttccacca tgcctaattc gaagaacaac 300 cgtaacggac acggcgagcc tcaccgccgc gagaaattga caaagaaatc gtcgtccttc 360 tacggtcact catcgccgcc ttccatgtcc ggcgaaccga tccggcggcc gaagacggtg 420 ccggatcttt tgtcggagag gcaccgcgcg gctttagcga cgccggcgac ggatgttgtg 480 ccaaaacagc cgccaaagtt gttgttgaag gttactccca tgggaagctt gggaccggtg 540 caggttgtga tgacgccgga atcgacggtc ggagattagt ggcggtgacg gtgaggcaat 600 acgt 604 // ID EH224974; SV 1; linear; mRNA; EST; PLN; 324 BP. XX AC EH224974; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5697.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5697 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-324 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; f3da63c619f7f55320d8533254edc79c. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: b column: 10 CC High quality sequence stop: 268 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..324 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5697" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 324 BP; 124 A; 46 C; 52 G; 102 T; 0 other; aattcgtata tagctgtata taatatcgat caaaaccaca attgagattt tgagaagaaa 60 acaaataaaa taaaataaag gagtagagaa agacaaggta gctgctagtt atttacgcat 120 aattctaatg gacacaaaga accaaataaa agaagaagaa aaaggtgttt aattaatttg 180 acggtcatgg ttaagctgag gataccaatt accaagattc tgtctctcat gaattagtat 240 actaccatca tcagttgctt taaaccgtgt gaggaacata tatctattga atctcccttt 300 tttctttgtt cattattatt cttt 324 // ID EH224975; SV 1; linear; mRNA; EST; PLN; 574 BP. XX AC EH224975; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5874.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5874 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-574 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; d722d66e147b737b81528398bd3ec8bf. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: d column: 5 CC High quality sequence stop: 573 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..574 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5874" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 574 BP; 201 A; 120 C; 82 G; 171 T; 0 other; cacaaaactc ttatttatat gatatcaacc taatgatatg aaataagatg tccttaaatg 60 cacgcatggg agataaatat aagataaaga agaatttaaa atatgcgtgt cactttacaa 120 catacaaacc tctttagagg agggcataca taaattgatc aacccaactc cgattacaac 180 acttcgttct ccagtatata tacacacaca catcaacatt caacaataaa cagaacataa 240 attaaatgtt gttgcaattg gctgttggat tttgcccatt tgccaacaca aattatgcat 300 aaacaagtgc catgttgcat taccaccttt tttttttttt accaaaagcg aggtcgacag 360 catgacctta atcgcaattg cataaaaaaa aatatgaaca atcaaaaaga agcttgtaac 420 caaagcttgt actagcattc tgcatagcgg cccccttccc ccttcaaaaa gttgtcaatt 480 tgttctagcg cctcaattgc aatatctgtt ggagacaatt cggacacatc tcttttgccc 540 agttttattg ctatattttt taatgagacc ctgg 574 // ID EH224976; SV 1; linear; mRNA; EST; PLN; 526 BP. XX AC EH224976; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6019.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6019 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-526 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; bab21ab6055dab6d34fb4544c537599e. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6019.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: e column: 18 CC High quality sequence stop: 483 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..526 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6019" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 526 BP; 161 A; 99 C; 73 G; 193 T; 0 other; ccacttcttg aattttttat ttattttcat ttttttaaaa aaaggcattt aactctctgt 60 ttcttgaaaa atacagtaaa aaaaaaaaaa aatagttcaa ttttttaagt agaaaaaaaa 120 accgccacca tttttttttt tggatatttc caaaatatcc tttaattttt ttttttggga 180 aatagtaata aatttagtaa agcacaaaca atacataaac gagatacaag cgcttaatac 240 tagtaaagat gagtttcatt tcatgtccat tactactgaa cctgaagaat tgataaaacc 300 tcttggtttc tgctctagca ccttcctttg ttagggtcaa cattgtattc ccagttgcca 360 taagccgttt ttccacctct atcgcaattg actatctctt tgttgaggcc atctgtacac 420 tcagttgggc caatgctcct atacccatct gagacgtgtt tgatatagta tctgaagctg 480 gtatctctat ctctgccgtt tctacaaacg gacttgtcct gatttg 526 // ID EH224977; SV 1; linear; mRNA; EST; PLN; 434 BP. XX AC EH224977; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5699.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5699 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-434 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 4e9c201c32f6f4a902d5571647408562. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: f column: 10 CC High quality sequence stop: 434. XX FH Key Location/Qualifiers FH FT source 1..434 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5699" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 434 BP; 107 A; 68 C; 137 G; 122 T; 0 other; tgacgaggtt tatgagaagg agtgggattt gatcatgatc gacgcgccga aggggtactt 60 tgcggaggcg ccggggcgca tggcggcgat attctcggcg gcggtgatgg cgagggacag 120 gaagggatcc ggtgtgacgc acgtgttctt gcacgatgtg gatcggaagg tggagaaggt 180 ttacgcggag gagtttctgt gtaggaagca cttggtcaaa ggtgttggga ggctctggca 240 tttcgagatt cctccaatgg gtaataacac tcgtgactac gcacgtttct gttagggttc 300 aggattatta cagtattagt attctttttt ttaccggtgt agtgaaatac attactggag 360 aagaatagtg agaggagcat atatatctat atgttactac agctttatta aaagaaaaac 420 tctagattgc tttt 434 // ID EH224978; SV 1; linear; mRNA; EST; PLN; 391 BP. XX AC EH224978; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5544.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5544 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-391 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; dab126c41530434b03b6d2466b227fbb. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: p column: 17 CC High quality sequence stop: 391. XX FH Key Location/Qualifiers FH FT source 1..391 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5544" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 391 BP; 122 A; 65 C; 73 G; 131 T; 0 other; ttcaagccta tcccgaattc aatttgaacc acaagtattt ggaagggcca aaaagggata 60 taaaatgggc gtcaaggaag ctgacagaca acgggtttgt gtacaaaaat gacctgaaga 120 tgatattgga tgattgtatc agatgcgcaa gaaggatggg cgacctctag tttttttaat 180 tctgaaagct tgcatgcgga tcctctcaag tttggagtcc ttctacaatc tgcatccttt 240 cttttgcaaa ttccatccac tgcattaatg acatttacgg ttattaataa atttgtccct 300 tccaatatta ttttaaatgt atctattatt tactttgaca gtggagaaac agatttgttt 360 cgtaatcaat tgataaatta tgatatatat t 391 // ID EH224979; SV 1; linear; mRNA; EST; PLN; 619 BP. XX AC EH224979; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6010.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6010 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-619 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 47fb4f1e1491af545f9f4a288d8e85b3. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6010.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: c column: 16 CC High quality sequence stop: 597. XX FH Key Location/Qualifiers FH FT source 1..619 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6010" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 619 BP; 194 A; 130 C; 145 G; 148 T; 2 other; tctagcattt tcggaagtag tcagtcctca caacctcatc cgagatccga tggcccaagt 60 tcatccagca gtaataagaa atcacaacaa gattcgcatg actcgtacca agaagtgact 120 gttggactga gtccctctcc actgtcacat gccgcaatga gatcccctcc agcagatatg 180 aatcttcgtc ataatcaatc gatgtcagag gagaagattt tagatctaaa caaggcatca 240 gaaacagtga attacaagcg ccagacgatg cctcaacctc cgcccttgat tcaacgtgcc 300 caatcaatgc ttgtggatag gccacagttt gcaatcgata atccagtgga gcacgaggag 360 gagtatgacg aagatgagga tgagggtgag attccagaga gttctgagct gaacaagcaa 420 aatcaatctg aaaaggtctc tcagaccact actgtgaaaa agctcactag ttcatcaaag 480 gaggaggagg acctgatggc tgaggttgat cagttcttgg caaatggaga gactgatgac 540 ttggcaaatg tgttggatga tgatggagaa atggaggaag ctcanaagct tctatctnta 600 aaatataact ttttttttt 619 // ID EH224980; SV 1; linear; mRNA; EST; PLN; 613 BP. XX AC EH224980; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5555.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5555 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-613 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b06c4eb48f47fc504597f469df5ab975. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: f column: 21 CC High quality sequence stop: 562 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..613 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5555" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 613 BP; 190 A; 140 C; 109 G; 174 T; 0 other; gaagtaaaat ctttcaaata taaatcactt taccttaaat ttaattttaa ttttgcaaaa 60 tcaaaattac tcaaaaactt tataagtgtt atgccagaca caaccactct gtaataactc 120 atttagtcgt aagacaaact gtttagtatt catttgaaca actattttta caactagtca 180 ctcacaatct cctatgtgtc ctaataaaat ttgttgtatt catcgcaatg gcgattgagc 240 aagcatagac tccaacggag cacagagctg cagatgccaa gagaagggct ttccattgtc 300 tcccagatac cagaattgcc agagcaacta atgcaactgc cacagctgaa agggcatttt 360 tccaagcttc gaaagctttg acaacggttc tacatccttt gcaatgaatc acatgcctaa 420 aatatctgtt ggcgaaattt ggggcatgca ttgtaccaat gccccccttg gtaggtgaag 480 aagctgattg tcccgcgacg agtcccgcag gagcatgttc aaccacagca ggctctttag 540 gcaaagagat agtgctgtga ccaaaatgat atggcattcc atgccccgct ttgtccatcc 600 acttcctgta ttc 613 // ID EH224981; SV 1; linear; mRNA; EST; PLN; 585 BP. XX AC EH224981; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6012.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6012 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-585 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 857fe9b60e2b338ec16cabbef8d743e1. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6012.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: g column: 16 CC High quality sequence stop: 585 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..585 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6012" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 585 BP; 180 A; 99 C; 92 G; 214 T; 0 other; atgaattctc ctttacctat cctttttctt aaaagctacc atttataaaa attaagttgc 60 ttaatctttg aagagatttt cagaaaatgt ctgacctact ttttcctttt aagaaaaaag 120 ctgaaaaaag gggtcaagca caaccttcat tgattattgc aacctggctt aatcagtgag 180 gctgtagtat aaattgagta gaagttcatg aacttagttg attagttatt atcctgtttt 240 taaagaattg cccagaaata aaaattacat tagagatata ttggattccc agtatctttt 300 gatgaaacgt ctgtcatcag ttcatatatt ttttatgaga agccaacttc attgttttaa 360 atagtcttcc gatctacttt ctacagaggc atatctatgg acatcaagga aagaacattc 420 agatctttgc agacaacagt aacttggctg tcaatccttc ttatgttcga tcatggttag 480 atgttttgtc ccaaacgcct cgagtagctc catttttctc aaaaagtgat ccttttgtca 540 tggcactgaa aaaagtatta ttgcattatt tttgcttttg cttat 585 // ID EH224982; SV 1; linear; mRNA; EST; PLN; 668 BP. XX AC EH224982; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5556.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5556 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-668 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3660758ae043201053f2343ba34ad31b. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5556.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: h column: 21 CC High quality sequence stop: 567 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..668 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5556" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 668 BP; 216 A; 158 C; 109 G; 185 T; 0 other; gggggcaaaa gcagatccat tttttttttt catttttcca tcaagaaata cattcagaat 60 cagtacatga tatgcacata aaagagagag acataaatta ttacatgccc taaagatctt 120 gctacctgat attcaaatac aaatcttaat caggattcta aaagttaaac aacctaacac 180 aattcttgaa gacccttttc tctctagaag agcccccaaa tcaataattt ataaccctaa 240 ttaggagtca gtaatgtgct taaggcccaa accgtgcttg ccacggacac acccaggcct 300 cacaaacaga taaggtgatt tcttcgaatt gtgcattttc gaaaatgccc ttttccctaa 360 tttcctcatc ctctgcctct caaacttgtt gctcacgttc catggaacaa gctcatcatc 420 caagccatac ccatcatcat cctcaccatc atcattatca ccacccacat catcatcatc 480 aacatcatca tggtgatagt gatgttgatg ataacggtag tggtgatttt ctaaacccga 540 ttcttgttcc gattcacgag gatcttgtaa acccacatcg ggatgaagaa gaagttggcg 600 aatttcttca attgaggcat cgatgggaga agaatcggat tcgaaccaag aaacggaaga 660 agatccga 668 // ID EH224983; SV 1; linear; mRNA; EST; PLN; 536 BP. XX AC EH224983; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6015.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6015 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-536 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 5c2543fa8faa5f686338fef4dcb1363d. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6015.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: m column: 16 CC High quality sequence stop: 513 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..536 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6015" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 536 BP; 141 A; 134 C; 110 G; 151 T; 0 other; aaaaagtact aaagttattt cattgaatga ttgtaagtta aaataacatt tattttgagt 60 ttttttttct aatacaattc tccgtaacta ttgcgaaacg gaaggagtaa atctcaataa 120 tggttcacga atacgtcgta ttccacgata gcgtctcctt tggtgaaatc aacagagtaa 180 aatctggctt gagcgtagcc acgtgcaaaa cgaaaagccc cagtgccgcc aacaatagcc 240 atttccctaa caggttcact catgatcatg ttcctcccca acacgctgat ggtgctgccg 300 ttgaaatctc catcggtgaa tgccatcgtc atcaccatca ttagtcccat ctcttcttgc 360 gatattgaag tgtaaattcc ttgggccttg cccacaagtt tcgagtcctg ttcgggcccc 420 acggttaaag ggtcgtccat cgccacaacg gttccgaacg gaagggcatc cttcgctttc 480 ccgttgggct cggcgataaa caccatgctg ggctttgggc ctgtcacgac atcatg 536 // ID EH224984; SV 1; linear; mRNA; EST; PLN; 635 BP. XX AC EH224984; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6015.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6015 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-635 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 041bc0647ed1b80bbfe6ec242ac401c1. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6015.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: m column: 16 CC High quality sequence stop: 583. XX FH Key Location/Qualifiers FH FT source 1..635 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6015" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 635 BP; 157 A; 157 C; 149 G; 170 T; 2 other; agagaggttc ctacagtagc aggaaatatc actcttcact ctcgagctat attttgcttt 60 cttagttccc aacaatatgg ccaaatccac tttctttgtc tgccttaacc tttcattact 120 cttctctcta gtcacagcca cttactactc aagtttaacc ccaacacttt tgggttttcg 180 cgaggagcag ttcacccacc tccacttctt cttccatgat gtcgtgacag gcccaaagcc 240 cagcatggtg tttatcgccg agcccaacgg gaaagcgaag gatgcccttc cgttcggaac 300 cgttgtggcg atggacgacc ctttaaccgt ggggcccgaa caggactcga aacttgtggg 360 caaggcccaa ggaatttaca cttcaatatc gcaagaagag atgggactaa tgatggtgat 420 gacgatggca ttcaccgatg gagatttcaa cggcagcacc atcagcgtgt tggggaggaa 480 catgatcatg agtgaacctg ttagggaaat ggctattgtt ggcggcactg gggcttttcg 540 ttttgcacgt ggctacgctc aagccagatt ttactctgtt gatttcacaa aagagacgct 600 atcgtggaat acgacgtatt cgtgaaccan ntatg 635 // ID EH224985; SV 1; linear; mRNA; EST; PLN; 521 BP. XX AC EH224985; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5718.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5718 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-521 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 5c5f80901b7a917b9d8b4aee82695c69. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5718.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: l column: 14 CC High quality sequence stop: 508. XX FH Key Location/Qualifiers FH FT source 1..521 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5718" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 521 BP; 145 A; 136 C; 105 G; 135 T; 0 other; aatacaccaa aacccaacat gaaaaccctt aacaaagcct cacttatttt attccctctc 60 ttgttccttt ccctattcaa gcattcccat gctgcaggaa tcgctatcta ctggggccaa 120 aacggtggag aaggcacctt agcagaagct tgcaacacta gaaactacca atatgtgaac 180 atagccttct tgtccacttt tggcaacggc caaactccac aactcaacct tgcaggtcat 240 tgtgacccca acaacaatgg ctgcactggg ttgagcagtg acatcaaaac ttgccaagac 300 cttggcatca aagtgttgct ctcccttggt ggtggtgctg gaagctactc cctcagctca 360 gctgatgatg ccactcaact tgcaaactac ctctggcaga atttccttgg aggtcaaacc 420 ggatcagggc cattaggtaa tgttatattg gatggcattg actttgacat tgaatctggt 480 gggagtgacc atttatgatg acctagccag gccattaaat a 521 // ID EH224986; SV 1; linear; mRNA; EST; PLN; 620 BP. XX AC EH224986; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5722.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5722 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-620 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 60cdd7abc9586b88c8efefdd1a7ae684. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: d column: 16 CC High quality sequence stop: 566 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..620 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5722" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 620 BP; 189 A; 130 C; 108 G; 193 T; 0 other; aaggtatata acatagtgtt gctctttacc ccttagcctc tgaatacaaa acttaacatt 60 ctcaaaagag acattggtgg aaaccttaca acttaatagt acaagaggca ccaaattaaa 120 gatgctttct cttattatgc tccaagctgc tattcacaat agctgacaaa agactatttt 180 actaacattt ttcatgcaag ttacacacct ggtcaccttc acttaagaaa cagtggtaag 240 gcttctatgg ctacagttga atgaaggtga tggttagttc catgaaagag atcggttgga 300 tatgtatggc tgtgatccga tttatttttg cagtctttat cttcgtcgtc ttcttcgaca 360 tcccaaagga aattagcata tgaagctagg acatagctat catctggggc acttttaatg 420 gcctgatcaa aatatccttc agcttgatca gcatccttct ctgtttgcca tattaactcc 480 gcatagaggg acagaatatg accatcatca ggattagcca aaatcgccct ttcgagatac 540 tcttttgctt taggataatc ttcacaaacc tctttcaaaa acttagcata atttcctagc 600 aaaagagcat cactggggtt 620 // ID EH224987; SV 1; linear; mRNA; EST; PLN; 692 BP. XX AC EH224987; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5562.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5562 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-692 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 7161878f62ad2a38fa7ca8b2193d8d07. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5562.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: d column: 23 CC High quality sequence stop: 597 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..692 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5562" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 692 BP; 238 A; 145 C; 114 G; 195 T; 0 other; agtgaagtga tatattatta accatgtata actttcatgc aataaacctt tgttgacaga 60 cacttgagat ataacacaac tacacaagta ggataaatta taggataaat tacaccatat 120 tgatcagtga tcacaaactg gtacataaac attattatta catcaaacaa aacaagctga 180 aaaatgaaca tgattatgac ttcaaactat cctacgactc tagcagataa acacaacttc 240 tttttgactg tagatgcatc ccatccattg aatgaaagga cgctttattg gctcagtcta 300 catcttagag gaagcctgca gcaatacaac gtttcactgt tgagacagga aacgtttctt 360 gaaaagatca acgatttcct gaggatcgac tcttgtctcg gtataagcat ttagcttatt 420 caactcctca aaccatgttg caagtttagg ccttccttct gtgatgtcat gtttgaacac 480 ctcagcaaag acaatttgga atctttcagc aaatggaata taggcaatat ccaccaaact 540 gaattgacca agcaagaatg gcccatcatc aaatttacca agagcattct ccaagtattc 600 aaaagcggga ctggcttgct gtacagcatc ccctttcaat gaaacgaaca ggtctctgct 660 gaatgtatca acatgggata tccaattgct ct 692 // ID EH224988; SV 1; linear; mRNA; EST; PLN; 433 BP. XX AC EH224988; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5720.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5720 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-433 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 4c395f644a78c4fd664bbdd4ac8a01fb. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5720.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: p column: 14 CC High quality sequence stop: 242 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..433 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5720" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 433 BP; 129 A; 104 C; 78 G; 122 T; 0 other; aaggaactcg gttgtcatta atctcaataa acactatcaa gtcacctgcc ttgtctacag 60 cctaaggcaa catgaaagca ctacaaacac tattgatctt atcaggaatt tggcagtgtc 120 agcactagta caaagattgt ccaagtttgg gttcttctgc agattctcca cataacaatc 180 tgcatgttta gattgcattg gtgtgtaatg aaaaccacaa ttcgtcacat acctctcatg 240 tgacattggc atgtcctgta aaccagggta atcctacttg aggacactgt tttgtgtctg 300 ctatcggaac aataggtccc agaaagatgc gccacaaatc aagtattatt gtgaccagca 360 ttgcagctcc caaagcatca acagccactc taggcaagtc ccatttatcg ccaggtttct 420 catctatcct taa 433 // ID EH224989; SV 1; linear; mRNA; EST; PLN; 325 BP. XX AC EH224989; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5882.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5882 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-325 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 90f4c5590f0a4e2db53655d0cf37ccfa. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: d column: 7 CC High quality sequence stop: 151. XX FH Key Location/Qualifiers FH FT source 1..325 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5882" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 325 BP; 81 A; 85 C; 82 G; 77 T; 0 other; tgtccgcaag ctcttcccct gctctggagg gaggggaagc aaggcttcta ctcactcact 60 cgttgtagaa gcttccaggc taccaaagct ggtttaccta ggaatagacg ttagatctaa 120 gaagtccaac tgtcgccaca cgccctgagc gtctggacaa gtaacgttct ctgaacgtta 180 cccgtcgatc tcaaaagaga gagactgacc gctcgtatat cgagcggact attactactt 240 gtgaggtgtc gagagaaatc ttcggcatcc agggcctccc aaaggaggga tgaggcttac 300 gcttcatctt tctcaaggat gacct 325 // ID EH224990; SV 1; linear; mRNA; EST; PLN; 308 BP. XX AC EH224990; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5559.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5559 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-308 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; aa1f788f441f90bebfc2fffa57ca51c8. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5559.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: n column: 21 CC High quality sequence stop: 256. XX FH Key Location/Qualifiers FH FT source 1..308 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5559" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 308 BP; 102 A; 77 C; 57 G; 72 T; 0 other; acccctttac ctttcttcat ctcaaacaca cttctttgcc atccaaaatc caaactatct 60 accaaactca attgcaccct acaaaagtcc caagtttggg gcacaaattt gagttgggtc 120 ccatcacaat cacatcatgg aagacctaaa ttggatgcag tgaggcccat aataacagca 180 gcggtgaaga gaaggaaaga gattcccttc gacaacgtca tccagaggga caagaagctc 240 aaatttgtgg tcaaggtaag gaacattcta gtgactcaac cagatagggt tatgtcactt 300 aagaccct 308 // ID EH224991; SV 1; linear; mRNA; EST; PLN; 471 BP. XX AC EH224991; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5726.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5726 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-471 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 40610737c0344c4826bcf806a867b7ca. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: l column: 16 CC High quality sequence stop: 465. XX FH Key Location/Qualifiers FH FT source 1..471 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5726" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 471 BP; 148 A; 66 C; 99 G; 158 T; 0 other; agtgaagtgg ctacaattgg aagaatacat cacgtgaatg tggtgcgtct tattggatat 60 tgtgctgaag gagaaaagca tgctctagtt tatgaattca tgcctaatgg ttcattggat 120 aaatacattt tctctaaaga agaaagtgtc tccttaagtt atgacaaaac atatgaaata 180 tgtcttggca tagctcgtgg gattgcttat ttgcatcaag attgtgatgt gcaaattcta 240 cattttgata tcaagcctca taatattctt ctagatgata acttcattcc aaaggtttcg 300 gattttggac ttgcaaagct atatcctatc aaagataagt caattatttt aactggacta 360 agaggaactt ttggttacat ggctccagaa ttattctaca aaaatattgg tggagtgtca 420 tataaggcag acgtctatag ctttggaatg cttttgatgg aaatgggaag t 471 // ID EH224992; SV 1; linear; mRNA; EST; PLN; 185 BP. XX AC EH224992; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5563.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5563 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-185 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 96bd6109eba8e889daa37121c56c61c4. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5563.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: f column: 23 CC High quality sequence stop: 178 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..185 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5563" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 185 BP; 68 A; 31 C; 33 G; 53 T; 0 other; cgaaagtaat aataaattct cgtatctagc tccagccagt taacctattt aaacaaataa 60 tcaaggaaac atttgtttcg atgtatgaaa ttttaagaaa agatacaatt tcaaatccat 120 gcggggaata gatactgaaa ctaaagggct ttcttcactt gaaagaagtc gggacggtcc 180 cttgt 185 // ID EH224993; SV 1; linear; mRNA; EST; PLN; 382 BP. XX AC EH224993; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5901.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5901 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-382 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; c2b554edb3400b8c123955579ee6730b. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5901.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: j column: 11 CC High quality sequence stop: 157 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..382 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5901" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 382 BP; 123 A; 104 C; 84 G; 71 T; 0 other; ggaaaaaacc caaacttttc ccctggagga aaggcaaaca ataatttttt tgggaattaa 60 agccaccccc cccaaggagg ggaaaaaaaa accccaaatg gcccttattt aaaaaactgg 120 gacctaattt taattggggg cctaattagg tggaaaccgg attagcccga aagaaatccc 180 caaaagccct ggggaaaccc ggagaactct ccttggcagg cttcacaaat tcttcgggga 240 ggtgcccccc ccccttggga aaaaaattgc ctggggacct cccaaaggaa cccccctctg 300 ggggtgcccc cagtttggac ccaaaaaaaa tcttccccaa agggtcccaa aaaggactgc 360 cttcaaacac actatagttg ga 382 // ID EH224994; SV 1; linear; mRNA; EST; PLN; 383 BP. XX AC EH224994; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5569.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5569 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-383 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 8e8efcf77c8b8fb81f2aba731440dc8d. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5569.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: a column: 2 CC High quality sequence stop: 338 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..383 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5569" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 383 BP; 102 A; 105 C; 67 G; 109 T; 0 other; accaaacact gaacttccag caacaatgca gtttgcccct gctgatgcgg ccacatctat 60 ggttgaaggc cctaaaccac catcaacctc tatgtcaagt gatggatact tcttcctaag 120 tatacgtact ttatccatca tctctggcat aaatttttgt cctccgaatc ccggttctac 180 agtcatcaca agaaccattt ccacaggatt tccggcttcc accagaggat aaacctcttc 240 aatgggggtc ccaggcttta atgctacacc aggagtcatg ccatgtgact tgattctttg 300 gataagttct tcccagttat cttttgatgt ctctacctga aatgtaaaac cagaagcacc 360 agcttttgcc aagggctcaa cat 383 // ID EH224995; SV 1; linear; mRNA; EST; PLN; 589 BP. XX AC EH224995; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5572.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5572 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-589 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; fe5cde926b283cd59fb652a91b4a4317. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: g column: 2 CC High quality sequence stop: 587. XX FH Key Location/Qualifiers FH FT source 1..589 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5572" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 589 BP; 192 A; 98 C; 112 G; 187 T; 0 other; aaaataataa ttagtagcat aataattaag ccaaagtgtt catagatatg gagggtcttc 60 tgcctcttgt ttataaagca atcaagagaa acaaaacacg aaggcaatac gagtgtcttt 120 catcaggaac ttctctaggg tacaacatca gcatggccga gatgtaccct caaactcagg 180 aacatgtcct tcagaaccag acacatgccc agaaagtgtt tcaccaccac cataatgaaa 240 gtcacaaaat tggtcacagg agatataact ctgttggtga tcatttcgat tatgggttcc 300 aatcttctca gatgagaaca ggtgttgatg ctccctcttc gaacaaactc gttaggttta 360 ggagtcaaag aatgttttca tgtatcactg gtgtttaagg tatatatatg tcaatttata 420 tatatcctat gatattagtt ttcccatgtc atatatatga tgttgcacaa tgttgtggcc 480 ttgattcaaa tgatgttgtg attcttgatt tgttccgtgg ctttgcttgg ttagttttgt 540 acaatgaaag gtagtatata ttgtgctttt aaaaaaaaaa aaaaaaaaa 589 // ID EH224996; SV 1; linear; mRNA; EST; PLN; 289 BP. XX AC EH224996; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5575.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5575 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-289 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 25543805ec0af30e7fb918726dfbe9cf. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5575.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: m column: 2 CC High quality sequence stop: 268 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..289 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5575" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 289 BP; 78 A; 42 C; 53 G; 116 T; 0 other; aagaatttta tcagtagtta gagagtgaaa atcaatgacc aattttaata tttgtttctt 60 ttggtttttt tgtaccattg acttttgaac ctctttgaat actttgccct ttaagattac 120 ttgaactgtt aagatttaaa actttagttg cacctttagc ttgtggcaca aatattgcac 180 ggagtttagt agcagtatca ccaggaaatt tttcatcatt gattgatctc tttgtggtgt 240 cacttttttg agccagtagg tttgacggtt gtgcaatatt gaacacatg 289 // ID EH224997; SV 1; linear; mRNA; EST; PLN; 688 BP. XX AC EH224997; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6027.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6027 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-688 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ec494974d78f4f90aaed1ee476b1f448. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6027.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: e column: 20 CC High quality sequence stop: 601. XX FH Key Location/Qualifiers FH FT source 1..688 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6027" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 688 BP; 207 A; 119 C; 130 G; 232 T; 0 other; aagaaattta agtgaggagc tagagaatga tgatgaagtc tgctatgtgt gcagtggtgg 60 ttgtagcact tcttcttctg gcggaggtgg ggcccatggc tgaggctgtg acatgcaccc 120 caacagagtt gagtccgtgt cttccagcaa tcacttccgg tgctaaacct tccaatgctt 180 gttgcacgaa gctgaagcag caaaaaccat gcttatgtgg ctacctcaaa aacgctagct 240 tgaagcagta tgttaactct cctaatgccc gaaaaaccgt tgcttcttgt gggattccct 300 ttccaacttg ttaattaatt aagccttctt ataattaaag tgtgggatat atatatatat 360 atagataaac agtttaattt atacacactt tgatcctaca aagcatcttg acacaagcat 420 tcaataggat ttcaccactt tatcgcaaca ttttaacatg atgcggtaat aaagtaaatt 480 cttgttgaat atttggataa cgatgttata tactcatagt ttgtgtgtat aaattaaact 540 tatatttatt atttactcta tatatgtcac tctacgtttg tgtgatatat tatacagtta 600 agtatttagc tatctttgaa tatgttaagg atgatcatat cagataaagc tctctagagt 660 tgaatatgac cagtatacat gttgtatc 688 // ID EH224998; SV 1; linear; mRNA; EST; PLN; 371 BP. XX AC EH224998; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6052.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6052 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-371 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 47cf6193b63d2f5d9f3268642b20a716. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: h column: 2 CC High quality sequence stop: 371. XX FH Key Location/Qualifiers FH FT source 1..371 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6052" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 371 BP; 132 A; 63 C; 53 G; 123 T; 0 other; atgaagataa caataaggat catccgtgct ctgttccatc tgtttgcatt atgtttctta 60 atgaaatcta caaataaatc ttgatgcatg aattgtttat gtcctgccca taagttgtag 120 cttttataat catagtcata gcaattataa gaggtactcc ctactcataa tgcaagtgta 180 aataaaacac tattgtgcaa catttaagtc aggctttctg cttgatactc actggcctgc 240 tacaaatgtc ttggaactga gaaaaatatt tacctacatt aaattaacaa ctgctagttt 300 atcacgcaat gtaaagatct atatgattaa tagagtattc aatttaattc agtatgcaaa 360 aaacaaaaaa c 371 // ID EH224999; SV 1; linear; mRNA; EST; PLN; 331 BP. XX AC EH224999; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5579.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5579 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-331 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 129dd6ca8655bb9455fa34e509652cbe. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5579.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: e column: 4 CC High quality sequence stop: 289. XX FH Key Location/Qualifiers FH FT source 1..331 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5579" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 331 BP; 98 A; 79 C; 68 G; 85 T; 1 other; tgagggaaag agaagccact tccaatttca cacacacaca gcaacacact gcactgtgtg 60 gaaagtaagc atggcaagag tggtggcact ccaacagaac caactatctt tctctccctt 120 agcttcctct ctttttgact tcagtggaac cagacttcaa actcaacttc agttcaagag 180 aaaactatgc catccaaaag ggtctttcta cgtctcggca tcgagcacca agaaaatcct 240 aataatggga ggcaccaggt ttattggtgt gtttttatct aggctccttg ttaaagaggg 300 tcaccaggtg actttattca caagaggtan g 331 // ID EH225000; SV 1; linear; mRNA; EST; PLN; 631 BP. XX AC EH225000; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5750.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5750 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-631 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 1e793aef046ca1ee6aaa141543060bd5. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5750.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: l column: 22 CC High quality sequence stop: 528 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..631 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5750" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 631 BP; 162 A; 161 C; 143 G; 165 T; 0 other; taaattatct aaaccaagac attaacttca atctactagt acaaaattag attaaaaact 60 tcaataaaaa gttctagttt gcttgggtaa cagcaacaca tccaccaaat ggaccagcgt 120 ttgcagcatt tctgcatcgt accaagcatg tttggccgtc agcaccacca gtgcaggtag 180 cgccagcggg catggttgca acaagtggaa attcagtagc ctttgccctt gatcgactgt 240 tcttgccagg aacgtttgtg tcgatgttca ttttcttgaa gttgtcacca gtggcggtgg 300 tgtcaacttc acagctgtat gggcctccgc cgtcaccgtt gacctggtga agagtcattc 360 tgacttttcc gtcaggtcca atactggcca atccggcaga ttcagccgat gacatgtcac 420 tagcaatatc gttgtttccg cccgcaagag tgcgtccaca agcagacgcc tttccgcttg 480 aaacctcacg gtctcgaatg atggaactgt cagttcggaa tgggttcgtt cgagtgccat 540 cacgaggagt ggactctacc attccaaagg cagatccagt ttgaccattt gcgccctcaa 600 ctgaagtaat aacaccgtga gccctggtaa c 631 // ID EH225001; SV 1; linear; mRNA; EST; PLN; 209 BP. XX AC EH225001; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5590.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5590 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-209 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 4ec9c916e499e3771e552f0d187d17b4. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5590.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: k column: 6 CC High quality sequence stop: 160 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..209 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5590" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 209 BP; 67 A; 43 C; 38 G; 61 T; 0 other; aagatgaggc accaaataaa aactatatat atatatatat agcaggatga cattaattgc 60 ttctcccctc gatttgagtg aaactttaga gtccagaaac atataatttt cgtcagcaat 120 tacgaatata tataggacat gctcgtaacg gtggcggata ccacggtata ttcgggttcg 180 atgtacatcc agtcacccct cctttctgc 209 // ID EH225002; SV 1; linear; mRNA; EST; PLN; 709 BP. XX AC EH225002; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5741.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5741 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-709 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 90545932e8c8c7fd559b52116da6ec61. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5741.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: j column: 20 CC High quality sequence stop: 652. XX FH Key Location/Qualifiers FH FT source 1..709 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5741" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 709 BP; 164 A; 225 C; 157 G; 163 T; 0 other; taactctttg tttcccactt gacccctaaa ttcgtgccca ttcccacaac tgcacattct 60 tctttctata tattccaaat cccttttccg atttccacca aaatctaaac cgaaattccc 120 aattaccctt cccaattttc accaaaacct aatactccca caccaatctt cgatcatgga 180 cgccgattca caacctcaac aactcgaaga gagaacagag gagcaagaac tgaagtacct 240 ggaattcgtg caattcgcga ccatacatgc tctgatgcgc tgcgccatcc tctactcata 300 cgccaaggag cgcgcgggtc ctctgaagcc cggtgtcgac accgtggagg aggccgtgaa 360 gaccgtcgtg gctccggtct acgataggtt ccaccttgtc cccggcgagg tcctcaaata 420 cgcggatcgg aaggttgccg agttagacag ccacgttccc tccaacgtga agaaggtctc 480 ctcccaggcc tgctccgtcg tctctgaagt ccgccgcgat ggcgtctccg cctacgccaa 540 gaccgtttac tccaagtacg agcctacggc ggagcagtgc gcagtgtctg catggcggaa 600 gctcaaccag cttccgctct tccctcaggt ggccaatgtt gtgttgccca aggctgccta 660 ttgcaccgag aagtacaacg aggcggttgt ttcctctgct gagaaaggt 709 // ID EH225003; SV 1; linear; mRNA; EST; PLN; 519 BP. XX AC EH225003; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5581.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5581 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-519 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 0b5d8c849ccc85b7fc97554e8943e100. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5581.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: i column: 4 CC High quality sequence stop: 498 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..519 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5581" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 519 BP; 138 A; 104 C; 212 G; 65 T; 0 other; acttaaacgc gtgcggcaac ggtgggaaca gcggtggcgg aggcggcagg gccgcggagg 60 aaggagagga atccggtggc ctgagagttg tcctggtcgt caagaaacag atcctccggc 120 acgcggaacg cgccgtggag gcagacgacg gcgacgccaa gcatgagagc ggagacgaga 180 acagatccaa cgctggtgag gaagacgacg aagacggtga aggcggagag gagcgctagg 240 gtttcgaaat ctgaaaaagt tctgccaaga ataacgagtg gttgatctga cggacggaag 300 aggtagagga aggtccagga ggcgaggagg ccgacgagga ggatgagaga gaaaggattg 360 gtgaggagag agactgcgag aatgagtgaa acgacggcgt agtagttgac gcggaagtag 420 gagaagttct tgcggacgcg gagggtggct tcggagaagg actcgggctt cgagaacgcg 480 gagcggtccc cgagctccga ccacgggcgg cgctggtcg 519 // ID EH225004; SV 1; linear; mRNA; EST; PLN; 398 BP. XX AC EH225004; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5749.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5749 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-398 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 9e71830ca729b083d653f3608cb95f46. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: j column: 22 CC High quality sequence stop: 393. XX FH Key Location/Qualifiers FH FT source 1..398 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5749" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 398 BP; 136 A; 71 C; 64 G; 126 T; 1 other; acgatcacag cagcacaagg aggaaccgtt tgatcctaat aacctcgtta ctttgctgag 60 ggcgggtaga gacggctcag ttgtaaacac tctaggattt gaggagccca atggtgttgg 120 ctccccagta agtgacatat caatccagct tcatcgtaac aacgctcttt gggagcttcg 180 ccccgtagta aagcaatctt aagtgtaaat ctctactata cgctttcaat tctcgcacgg 240 gttttcaagc atttttttta tacatttaaa ttattttaag gaaaacttta tttttgttat 300 ctttctcttg aaaaatcttc tttcgtcttt gaaattcttt tttgtttttt aataaaaaaa 360 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaan 398 // ID EH225005; SV 1; linear; mRNA; EST; PLN; 429 BP. XX AC EH225005; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6036.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6036 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-429 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 34ea4aecd9bed406392e3c5298c6af2f. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6036.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: g column: 22 CC High quality sequence stop: 429 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..429 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6036" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 429 BP; 135 A; 73 C; 70 G; 151 T; 0 other; ccagtttaca tctatgcatc ccctcctcct ccatatcact actaggttat ttctgaagaa 60 tataagccaa taaaaaacgt tgttgatagc aggaagatgc acttcgcaat tgcacaagga 120 aaacaacgga agccaagttt ccgcgcattt tgtcacataa gaaaaacgaa gtgtcaatcc 180 ctttcttctt cgtttgtatt gtttatgaat tattaattac tttggtttta gacagttgta 240 tggcactgct tttgtctctg tcgttgaatg atatcacatg aatgcatttt ggtttgcaac 300 ctgctgcgct tgcaaggggt tgtgaagcca attttataat aaataaaatc aaatatatgc 360 tttgtatatt tattgaaaaa ttctacaata tgtattgtat cttcaataat aaaaatttga 420 tttaaagat 429 // ID EH225006; SV 1; linear; mRNA; EST; PLN; 322 BP. XX AC EH225006; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5580.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5580 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-322 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 25e940a64f2b2bb53912ea45929d57cd. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5580.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: g column: 4 CC High quality sequence stop: 183 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..322 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5580" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 322 BP; 116 A; 82 C; 47 G; 77 T; 0 other; aggtccgaaa gatcactatc attatcgctg aacaatgtta tttaacaaaa gtatcaacca 60 caaataaaaa caacaaccca acttcccaat catcgtcacg tccagcaaaa tacattcaaa 120 agctatctaa aatctgggga acccacattg gcattaaaaa ccatgaccat acaatttttt 180 agagaactat tatgggcacc atgcctcttc aacaatcccc taaacgttgc ttaggcatca 240 gcaaacccaa gctcggaaag cttttggtga gcctcagcgt aatcagcaaa gaaggcatct 300 tcgtccgctg catatttatc aa 322 // ID EH225007; SV 1; linear; mRNA; EST; PLN; 574 BP. XX AC EH225007; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5748.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5748 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-574 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 5882f04d4be6e55350ce8bd250e5a445. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5748.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: h column: 22 CC High quality sequence stop: 491 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..574 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5748" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 574 BP; 233 A; 124 C; 71 G; 145 T; 1 other; ggcaatgaat ccttgtaagc tcatataact taattcctta aatgaaatca tgagactaat 60 gtacaaaagc aacacaaaat ccctagatca aacacggttt tctatttcta cataatcaaa 120 tagcatgacc caaaaatgaa gaagcaataa ccaaacctct cagttcccat tattatgaag 180 attcatcaac ttcatacaaa acaaccagtt cagatttaaa ggccaaacta caaacaaaat 240 gcttagaaat taaaaaggaa aataaactag atggatcaca atcacaattg gctccctcaa 300 gctctagaag ccttccttaa ttctctctcg gtctccaaat ccaactgtga gagatgcctt 360 ctcacctcca aattaaaagt gtctctttgc attttccacc tcaacaagct ggcacctaca 420 attcagaatt tttctgccaa aaattaaaat atcgatcctt caaaatttgt cctcaactag 480 aaataataac agaatagaga aaaaattgga atgccaagaa aatcaccaac actaaaagct 540 atatcanaaa gtagaacgtg agatagaaaa tagg 574 // ID EH225008; SV 1; linear; mRNA; EST; PLN; 691 BP. XX AC EH225008; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5759.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5759 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-691 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ec270cf9e1cb4a81161d80e8be9b9f55. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: n column: 24 CC High quality sequence stop: 659. XX FH Key Location/Qualifiers FH FT source 1..691 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5759" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 691 BP; 181 A; 140 C; 167 G; 203 T; 0 other; acaaagtgag cacattatca gaagtgctca atcgccaaac gaggttgggc gagccatttg 60 tgaaattgtg attggctcca taactgccac ctgtcaatcg gagctagatt ctctggagcc 120 tggaaagtaa gatcagggaa ctcagttgag cccattcaaa aaattggtat atccttcatt 180 ttagcttctt cagtcgacta ttctgtgaag agttgggttt atctcctacg tcaacaggcg 240 aagacatttc ggtttggtag gttgaaatca agaacgtgaa catgcctcac cccctgtgga 300 tatcattcat cctctctgta ctgcttcaag ttataccttt ggtttggggg cactgtaaga 360 tcgttactgc tgccggaaat ttaaatactt caagtcaagt cagctatgga tttggtgtgg 420 atttacatag caagtacccg tggtttaagt ctcaagccgg tgatgccgga gcggattctg 480 aagtttttac tgaatcgaaa gagtttgtcg ccaatcctaa tcccccttgt ggcatgcgtg 540 cgaaaatggg tgccctagat ttcgactcat cttttagtca agccgaagca atgggcgtag 600 gaaatacttt agaagatggg tcttttgagg cattgatttt tcaggtcaac cgtgatggtg 660 gaggacaaat gcactttgtg aatacaacac t 691 // ID EH225009; SV 1; linear; mRNA; EST; PLN; 643 BP. XX AC EH225009; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5751.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5751 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-643 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 84a6a2c580adc94b843f2017b1c1b611. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: n column: 22 CC High quality sequence stop: 643 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..643 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5751" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 643 BP; 206 A; 157 C; 99 G; 181 T; 0 other; gctatcttaa ttcttataac tgataattaa agcggggcgc atcaatacaa tacaatacaa 60 tatgtaacta tgaactatca atatatgatc gttatttatg tctgtgatac acacaattca 120 ggccatgata ttcttacgtg atttacagca acttctaact tatttaatac atcataccaa 180 cccaactagg agcaattttc attatacaat gacacttatc tttaaactct gaagcatgac 240 aaggtggtag tcaccaggga ccatattctt atgcaattta tgccacaact ggataagacg 300 cacaagttgc aacaccacac atgttcttcc ccaattccat tttgaagtag ccattctcac 360 cccaactttc tccccatgaa ttttttatga gccaatatgg gacgccattt tcaactccat 420 accccacagc aaggacggca tggttcacat cctgggaagt gctaccgcaa atgtcactag 480 tgtaaactcc attctcgtag aaatggaacc cattcaccac ctgaaaggcc acactaaccg 540 gccgaacaaa tgcaactgca tgttttaatt cattctcagc acccaaggtg atattgaccg 600 agtcaatgac ttgaacggca acattttcag ctgagaattt gca 643 // ID EH225010; SV 1; linear; mRNA; EST; PLN; 509 BP. XX AC EH225010; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5753.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5753 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-509 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b15c20e365dc2e664b30726448070edd. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: b column: 24 CC High quality sequence stop: 485. XX FH Key Location/Qualifiers FH FT source 1..509 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5753" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 509 BP; 203 A; 78 C; 106 G; 120 T; 2 other; atctgggaga gcaagctcta ggttgacaac atataggttc ccattgaagg aagagttgag 60 gccaaggctg aatcacaaac ctgttagtga ccccaatagc aagttgaaga tgggagatgt 120 gattgaactt acgccagcca tacctgacaa gtctcttaca gaatacaggg aagaaatcca 180 gcgtatgtat gatcgtgggc taactgtgtc gagcatggga accgctgcaa gcaccatggt 240 tggctcaagg agttgacccg tgcttttgga tttcacttgc attccaacat gaaattattt 300 agtgacaagt tatgtatata gaaaatattt gtacagaaca actgtgttac tactttgaac 360 ttggctaact tgattcaatt atgtgctgaa tgattgattt tatgtaaaaa aaaaaaaaaa 420 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 480 ggggcggccg ttctaaagga tccaagtnn 509 // ID EH225011; SV 1; linear; mRNA; EST; PLN; 621 BP. XX AC EH225011; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5586.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5586 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-621 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3c150b66f1f7db690cfa84471c0f83e5. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5586.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: c column: 6 CC High quality sequence stop: 502 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..621 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5586" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 621 BP; 163 A; 147 C; 95 G; 216 T; 0 other; ccaaataatt tgtcaatgtt gcttattcga gttccgcata aaatgtttta caaatcaaat 60 aaattataat ccatttacat ttctcttgct ttcatctcac caaatacatc catgatttaa 120 gttttagtgt tgttatattt cctctactca cccaactaac accgaagaca aataaataaa 180 acagttgaat ccctggtgtt acacagtcaa tttggtgact ttggttccca atggccacca 240 agagatatgc cttcacataa ttaaaggcca tgtcttgtgc caagaatgag aggcaataaa 300 ttctgtggct ttttctctca accaaatgga ggaacctcta atccttttat gctataagcc 360 tagttccttt ttcaactgag ccagagtggc ttctatttcc tcaaggttat cctcaacgcc 420 tctgcttccc ttgccagctt gaccttgact gctattgctt ggtgtctctg tcccaggaaa 480 gttcgatgtg ttgcctccct gaggcgtttt ataatcatca ttttgatcag taatattgag 540 ttctttctca acgaactcca caaattcttc tcctatttcc ggcaattcct tccatagact 600 ctttggcttt ccttgtgaag c 621 // ID EH225012; SV 1; linear; mRNA; EST; PLN; 416 BP. XX AC EH225012; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6042.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6042 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-416 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 1c2baef7e645f489aa30c4ba2e58ce49. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: c column: 24 CC High quality sequence stop: 416. XX FH Key Location/Qualifiers FH FT source 1..416 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6042" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 416 BP; 99 A; 103 C; 109 G; 105 T; 0 other; cttaggatta ggtaaatgaa taccctccta gagactcttg cacgacggga tgtccgcaag 60 ctcttcccct gctctggagg gaggggaagc aaggcttcta ctcactcact cgttgtagaa 120 gcttccaggc taccaaagct ggtttaccta ggaatagacg ttagatctaa gaagtccaac 180 tgtcgccaca cgccctgagc gtctggtcaa gtaacgttct ctgaacgtta cccgtcgatc 240 tcaaaagaga gagactgtcc gctcgtatat cgagcggact aatactactt gtgaggtgtc 300 aagagaaatc ttcggcatcc aggtcctccc gaaggagggg tggggcttac gcttcatctt 360 tctcaagggt gacctagagt agcggtttgg gggtgtcaaa ccccgttttt ttgatc 416 // ID EH225013; SV 1; linear; mRNA; EST; PLN; 306 BP. XX AC EH225013; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5590.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5590 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-306 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3b6394918de10b1a2136d8d464f47baf. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5590.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: k column: 6 CC High quality sequence stop: 306 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..306 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5590" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 306 BP; 87 A; 56 C; 50 G; 113 T; 0 other; accaaaacga gcatgtccta tatatattcg taattgctga cgaaaattat atgtttctgg 60 actctaaagt ttcactcaaa tcgaggggag aagcaattaa tgtcatgctg ctatatatat 120 atatatatag tttctatttg gtgcctcatc tttttaacac tattcctgtg ttaaaactcg 180 gtggtgtcac atctttgctt taaaattccg tgttcttttc cagtttgcag ttgcaataat 240 cttctaatgt tgtactgagg caaaggcttg cccttttagc cactcaataa atatgagaac 300 ttcatt 306 // ID EH225014; SV 1; linear; mRNA; EST; PLN; 634 BP. XX AC EH225014; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5587.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5587 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-634 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 0d1cabf779308ec722c90462e0c216b2. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5587.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: e column: 6 CC High quality sequence stop: 626. XX FH Key Location/Qualifiers FH FT source 1..634 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5587" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 634 BP; 213 A; 107 C; 130 G; 184 T; 0 other; caagcagaga ctaacagagg agtagttaga taacatagta atagaatgaa accctcctga 60 ctcacagtca cagttaacta atctgtaagg aacaaggaga ctgagctcta atttttaagg 120 atacaacgac ttacattgag gcaaattttt acgatctatc accatgtttg atctgttatg 180 agctgataca gtgttttatg actgaatttt gacgaaggga tttgattagt tatgaagtgt 240 taaagtctta aatagttaaa ctgacttctt ctccatgttc tcttacaatt tcttcaaaga 300 tgatgatcga tcaaacagcc gttatgatag gaagtgagga tagataagat ccaactgttc 360 agagagaggg tataagagta aataaataga atctttcacc agctgcagat ggattagaac 420 ttcagaagtg aaacaggaca actggctgac gttgtgatgg ggaatgtttt gtgggatacc 480 acaattctca gaagtttatc agttaggttt atgcgtcttc ttaaaaaact acgacagaca 540 atgccatttc aattcaaacc agggcaggag agagacaact tcgattcact tccactcata 600 ccttttctac tccatggtat gagagtataa acgc 634 // ID EH225015; SV 1; linear; mRNA; EST; PLN; 296 BP. XX AC EH225015; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6047.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6047 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-296 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e98292b41c75df8f64d4f29680b6d322. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6047.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: m column: 24 CC High quality sequence stop: 296. XX FH Key Location/Qualifiers FH FT source 1..296 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6047" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 296 BP; 108 A; 48 C; 65 G; 75 T; 0 other; actaaagttt gagcttgaga gtgtgaaagc ccagtacatg gagcttcaaa atgatatggc 60 tagcttgcag aaacaatttg ataagatgct aaagcagaag catacttctg catggagcag 120 tgggtggaag aaactcagca aacttggaag gacaacacat ctagtagaaa atcaggatga 180 ttctcctgag attcaagatt cacttgaaca aaacagaaag actactagaa ggtggagaaa 240 ctcaatatcc tgattcctga aatatgagaa ttcactattt ctttgaaagt gtttct 296 // ID EH225016; SV 1; linear; mRNA; EST; PLN; 603 BP. XX AC EH225016; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6048.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6048 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-603 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 0ffe13e42fb057b56d89e8c8009bde2a. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6048.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: o column: 24 CC High quality sequence stop: 487 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..603 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6048" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 603 BP; 192 A; 124 C; 113 G; 174 T; 0 other; agaatacttc tttcattttg gggaaatatt agttttggta gcatggggtt taaataatag 60 aatagcaacc aacgctgcca ctatactatt actaaggtgt gattttaaac aatgcttgaa 120 attgtaaaac ttttgacaga acatcaaatg aaaattttaa ataaaaaatt attgacataa 180 ttcagagata atagaacacg gcatgaattt ggcttagggg tcaactatcc tataaacctt 240 ggtaaattag ttcggcatga atctatcagc cttctctatt ttgattgctc tcaaatggaa 300 ctcgaacaaa caattgtcca tgcaaggtca ccacagccga cgtctgctga gtatcaattc 360 ttgaaggcaa gctgacaacc ttcttgaaag gtgtcacccc ccatggattg tcagaatgct 420 cgggctcacc acttatgacc aagcgtccag ctgggtctga ttgaacacgc acctcttcgc 480 gcagaaggcc aggaactaaa gcatagacct caaaacaatc tttagttttt tgcacattga 540 tttttaccca atcagcagga ggtccaatgt caaccacaga tgtgtccgat atgtgtctta 600 act 603 // ID EH225017; SV 1; linear; mRNA; EST; PLN; 280 BP. XX AC EH225017; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5602.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5602 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-280 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; dbe1e52dee14613c883d6321524669e2. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: c column: 10 CC High quality sequence stop: 280. XX FH Key Location/Qualifiers FH FT source 1..280 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5602" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 280 BP; 46 A; 101 C; 107 G; 26 T; 0 other; ccgacggggc gtcggccgga tcgttcaggt ggaggtcttg cgccattcga gtaccagcag 60 aaatgcgaga tagacaccgc cgatgccggc ggtcaagacg cccacgggca ggccgttggg 120 accggccagg cgggtcaaca ggtcggcgcc agccagcagc accgcgccgg tcagggccgc 180 cagcaggggt tgcggaccgc tggccggcac gcagcgccgc gcgatctgcg gcgccgccag 240 cgcgacgaag gccaccgggc cggccaccgt gaccgccgcc 280 // ID EH225018; SV 1; linear; mRNA; EST; PLN; 524 BP. XX AC EH225018; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5607.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5607 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-524 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; f25dc7ea5964a0097d33bbc617a415e9. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: m column: 10 CC High quality sequence stop: 209 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..524 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5607" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 524 BP; 146 A; 121 C; 91 G; 166 T; 0 other; atttaaatgg tatacatact acggaattct aaaagaagat ctaacttaca gttactcccc 60 tttttttttg gatccacaca taaatttgac ccggcaatcc attattcatg agactaaata 120 ttcaagcaaa ggcacggtgc cgaagaggtg gcctagacta tcttcgaaaa cttgccttac 180 atttggtcag tctttactat gaagttcttt tttgctccat ttcctgcagg ggtatttgtg 240 gcacaactca gccattactc tttatcggca tcaacctttg aaggggtttc atcactttta 300 acagctggag tttctaattt gccatcttca gtatcagtag gcttggcagc agcatcaata 360 tcacgggaag tcgattctaa tggttcacca atggactctg gttcagttac tggaacttct 420 tcacttttaa cagttccaac cttcacatac ttgctcataa acttatattt cccagtcttg 480 taaggcatca agctcaaacg ggccaaaacc agagatatcc ccag 524 // ID EH225019; SV 1; linear; mRNA; EST; PLN; 458 BP. XX AC EH225019; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5600.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5600 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-458 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 7736431e33997cfdfca2a7cd055cedc8. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: o column: 8 CC High quality sequence stop: 402 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..458 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5600" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 458 BP; 132 A; 96 C; 103 G; 127 T; 0 other; atgaaagaca aaaaatgcct tatggcttta caaaaaaaca aatttgaggt agaaaatgaa 60 caatcatagg agctcacact ttgatgctta gagtttacat tttagaaaag ctcttgttgc 120 accacagcca acacctctca gctaacccac caggataaga tcatccagag tccaaaggca 180 gcaagcgtag ccttgagcac gggtttattg tttgcgagtt gggattgacc tttttggact 240 ttagccattg cttgactggc cctctcagca ataggcccgc ccgcaggctt ggcggttaat 300 gccttcttat tttcaattgt atcttcatca gttttagctg agattacagg agcaggcaga 360 gagtcacttt tgggcgctcc catgtttggt aagtcaacaa caatgttagg gttggaatac 420 tcaggaacaa ttgtgcggga atttaacttg agagacct 458 // ID EH225020; SV 1; linear; mRNA; EST; PLN; 600 BP. XX AC EH225020; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5916.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5916 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-600 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; bc60b2b585f44d1fbcb6b18a51712c16. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5916.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: h column: 15 CC High quality sequence stop: 595. XX FH Key Location/Qualifiers FH FT source 1..600 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5916" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 600 BP; 127 A; 188 C; 142 G; 143 T; 0 other; cactcgctag ccacaaacca gttccatttc atccattcca actttccaac cacaacacac 60 tgtgtgtttg taaaaatggc catcacaggc aaccttaact tcaacctcaa cctagtactt 120 cctgtggcac gccatatggc gtgccctcca cgcgtcccgt cgtttctctt cactcgcggt 180 cgaggcgcgt ggtccccctc gcttctggtc acgcgcgcca tcgacgctaa cgagtttctg 240 ggggatttcg gggcgcggga ccctttcccc gccgagcttg aaagctcgtt cggggaaaaa 300 gtgctgggat acggcaacac tgagcacaag attcttattc ccaccattac tgctctcggt 360 ctctcgcagc aggagtgtgc tcccgtttct tctgcgcagc cccccatctc gctggatgac 420 gcccagactc tcattagaaa gctatgggag agggatgttg tacgcagcct tacccttgca 480 tatgcaaaga ggctgtttcc ggattcgaac ccatgaccaa caagtcacca aggcacaact 540 ttaccgctgc atcagggctc gccctcaagc tcaaggttgt gggttggaga cttgtgaatg 600 // ID EH225021; SV 1; linear; mRNA; EST; PLN; 520 BP. XX AC EH225021; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5763.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5763 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-520 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3975eaaf78a2f89f6261d615bb130d03. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: e column: 1 CC High quality sequence stop: 346. XX FH Key Location/Qualifiers FH FT source 1..520 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5763" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 520 BP; 140 A; 127 C; 136 G; 117 T; 0 other; caatggccac gcttagcttc tgcgtcgcca cgacatgtct gagtgctgcc gctgattatc 60 gtagaagaag caactggaaa tggagcagac ccacaattgt gtgtgttgga tgggacccgg 120 aaggcgtgct tggtccaccc caaactgggc acctcgccag gttctagttt gataggcgcc 180 tcgagagaga cgcagatgcc cgcgaagcct tccaacgtca agtccgtgaa gagaaagaac 240 gccgtctttc ccttcgacaa tctcgtcctc ttcccgatac tccgcaagac ctcattgaat 300 acttccatga cactgaagct caggagattg agtttgagat tgccagattg agaccaagat 360 tgactgagga gttctttgcg caactcatat tcgagttagg acggcttcga ttcgctgtta 420 acaaaacaca gctcatggag gacagacaga ttgagctgga ggctctagag aaagccatac 480 tagaaggatt agaagcctat gataagatgc aaggtgaact 520 // ID EH225022; SV 1; linear; mRNA; EST; PLN; 608 BP. XX AC EH225022; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5617.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5617 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-608 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 924d7f5722d7b1b5b8a465d9f7c1e5f5. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: a column: 14 CC High quality sequence stop: 608. XX FH Key Location/Qualifiers FH FT source 1..608 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5617" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 608 BP; 156 A; 197 C; 142 G; 113 T; 0 other; tgaaaaacca caacctagaa agaaatgaag aaaatacagc actctgaaca aaaagcaacg 60 gaaccaacca aaaaggattc aaagggcaac aaacaagaag gcacagactc agagtcagag 120 caataccaaa acgaagaaaa tcaactccac acgctttcgg aaccatcacc atccttctct 180 ctccgttccc gcaaatcccc ttcgccacca ctccactccc tctctgactc ccccatctcc 240 gatgcccacc tctcccctgc gccgcagaag ccgccgcccg attcctcgcc ggactcctcg 300 atctccgacg gccacttcac gatcggtgaa cagcgcctct ccccggcgcc gccgaccgtg 360 gtgacggctc atcggttgaa ggtggagccc gcggtgtttg accagggtcg atcccggcgc 420 cgaagagggt ttcgtgaaag acgtcgaaca agccaccggc agcgccggta gtcgacgcct 480 cagaccggat atgaccggtt tgttgaggtc gaaaaaaatc gccacgtgga gcaagctctt 540 gctgggtctt cgagtaaccg cttttgtttt ctgcttggcg tctttctcgg tgttggccac 600 tgataaga 608 // ID EH225023; SV 1; linear; mRNA; EST; PLN; 635 BP. XX AC EH225023; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5917.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5917 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-635 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; a29dee69a381d5670e5cce84f43e9a12. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5917.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: j column: 15 CC High quality sequence stop: 633. XX FH Key Location/Qualifiers FH FT source 1..635 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5917" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 635 BP; 161 A; 153 C; 154 G; 166 T; 1 other; tttcttacaa ggctctactc tactgcttca ccttctgctc tagaaaattc agtggtgata 60 catggcttcg gctactctct ctgtagccaa accagccctt caggcaaatg ggaaaggctt 120 ctctgaattc tctggcctcc gcagctcatc aggcttcctt cccttttcta gaaaatcttc 180 agaggatttc cattctgtca ttgccttcca gacctatgca gttggaagca gtggaggata 240 caagaagggt gtgacagaag caaaactgaa ggttgccata aacggatttg gaaggattgg 300 aaggaacttc ttgaggtgct ggcacggtcg caaagactcc cctcttgatg tcattgccat 360 caacgacacc ggtggcgtga aacaagcctc tcaccttctc aagtacgatt cgatcctcgg 420 aaccttcgat gctgatgtca agcctgttgg cagcgacatc atctctgtcg atggaaagga 480 aatcaaagtt gtctctgacc gcaaccctgc caaccttcct tggaaggatt tggggataga 540 cttggtgatt gaagtaactg gngtgtttgt agacagagaa ggtgcaggga aacacattca 600 agcaggagct aagaaggtgc tcattactgc tcctg 635 // ID EH225024; SV 1; linear; mRNA; EST; PLN; 226 BP. XX AC EH225024; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5601.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5601 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-226 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ffb8696a5115686303db315c8907d7b9. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: a column: 10 CC High quality sequence stop: 226. XX FH Key Location/Qualifiers FH FT source 1..226 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5601" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 226 BP; 60 A; 36 C; 38 G; 89 T; 3 other; atttctaatc atcatgccat cataggtcta gctagaagca agtcgaggag ggtatgttta 60 attgatgatt tgaatgtgta cttaatatag ttctttttac tcgattttgt tacttaggac 120 cttcttcatc aattctcttt tctccattta aacataccct gaatgtaagg taatgttcag 180 atactcttta tgtcaatttt gatctgacca ttgagtggga ttannn 226 // ID EH225025; SV 1; linear; mRNA; EST; PLN; 564 BP. XX AC EH225025; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5928.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5928 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-564 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 1da8bea656d8aafbc70a9457753ef603. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5928.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: p column: 17 CC High quality sequence stop: 551 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..564 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5928" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 564 BP; 202 A; 121 C; 103 G; 138 T; 0 other; ggaaaaaaga aagggatgtt aacctctaaa cgacattaac atccttatat aatcattaca 60 taaaacaatg ttggaataat tgacctctta agactgctga tggacagaac aaaatgacaa 120 gtggacgtgt acaatatggt tgcattcggg cttcccgccc aaaattatca tagcttgcaa 180 gagcagccag caaatttgag ctcagttacg taataaacta cttgacattt gatcaaattt 240 ctcttaattg agagtacatt tgatgaaact ttgaaaaaga tactgataaa gtatcaaaag 300 gatcactaac tactaatatc tcttagattg gctgaccttt gggaacaact tgatctttga 360 gccacatgta aattggaacc aacacaaacc atgtggctgc caaggcaccc aaaacgaagc 420 gcagtgcaaa cacaaaagga ttaacggact tttcaacatc ttcttccttt ggaccaagct 480 gcaaaggaga atctccagat gcaggcttaa caccggtcac tgaacaaccc ataatcaagc 540 catgtcggcg aacaccacgg tacc 564 // ID EH225026; SV 1; linear; mRNA; EST; PLN; 434 BP. XX AC EH225026; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5919.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5919 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-434 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 2a7ed2243eb11dd7bb74294bc50d6bdc. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: n column: 15 CC High quality sequence stop: 434. XX FH Key Location/Qualifiers FH FT source 1..434 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5919" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 434 BP; 100 A; 107 C; 111 G; 116 T; 0 other; acctcttcgg tcttatcgct taggattagg taaatgaata ccctcctaga gactcttgca 60 cgacgggatg tccgcaagct cttcccctgc tctggaggga ggggaagcaa ggcttctact 120 cactcactcg ttgtagaagc ttccaggcta ccaaagctgg tttacctagg aatagacgtt 180 agatctaaga agtccaactg tcgccacacg ccctgagcgt ctggtcaagt aacgttctct 240 gaacgttacc cgtcgatctc aaaagagaga gactgtctgc tcgtatatcg agcagacttt 300 tactacttgt gaggtgtcaa gagaaatctt cggcatccag gtcctcccga aggaggggtg 360 gggcttacgc ttcatctttc tcaagggtga cctagagtag cagtttgggg gtgtcaaacc 420 ccgttttttt tggt 434 // ID EH225027; SV 1; linear; mRNA; EST; PLN; 587 BP. XX AC EH225027; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5921.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5921 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-587 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 19709b38d79de79549fb74ff42fc9d10. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5921.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: b column: 17 CC High quality sequence stop: 553 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..587 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5921" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 587 BP; 157 A; 130 C; 108 G; 192 T; 0 other; ggtttgttta cttgattatt tcatttctaa tattttgttt ggtacgatag ttcagctaca 60 agtaacagcc gcatttagat ttcttttatt cttacaagac atatgacgag gataaaaaaa 120 gttggtaaag ctcttggaag atatcaaacc caacgatatt ttgttgttgt tttttttaaa 180 aagatatatg gtacactgcg acatatttca cgttgcacca agttcctgat ttttttaagc 240 tgaaacagct tgaagcacac caccatggat agctacatag gcttgaatct ccttcatctt 300 ttgttgcttt gccagacctt cggcctcacc tccctcatca gctacctccc attggacaaa 360 ctcgttggtg tagttgctta caaagtctgt tggaacaatt cccaagcgct ttgagtagtt 420 ggtcaccttg ttccagtcac gagccacatt tgctaagtcc ttgctcatgt agttaaagct 480 acgctcaaac atcaatcgat tcaagggagt gttcatagga ggcttaaatt tgcagtactc 540 taaccagctg ccacgtggat cagcaaacat atcatccgct ccggcct 587 // ID EH225028; SV 1; linear; mRNA; EST; PLN; 392 BP. XX AC EH225028; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5800.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5800 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-392 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ebe963beb9e247339494d571469293db. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5800.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: o column: 9 CC High quality sequence stop: 369 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..392 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5800" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 392 BP; 128 A; 80 C; 66 G; 118 T; 0 other; ggagggaaac atccaacgat aatttaactt gttatattta caaatttatg actcaacagc 60 acccttgaag tccatcaaaa ccaccaatcg caagtaatgc acctcacata tttggcatcc 120 tcggctggaa gattgtatag gaaaagaaaa acaccataaa taatcaattg gcaagactac 180 aaatattttc atgttcttga tacggtagaa ttccaggttg ctccaatcaa agtgccaact 240 ctttctcctt taataacttt ctcaatgtta ccaggtttgt taagattgaa gacaacaact 300 ggaatattat tttcttggca taaagttatg gcagtcatgt ccatcactga cagatctttg 360 gaagttacct cccgataggt tagtgtctcg tg 392 // ID EH225029; SV 1; linear; mRNA; EST; PLN; 547 BP. XX AC EH225029; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5779.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5779 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-547 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; efd05462a23ecac67262b7438b645bfb. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5779.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: e column: 5 CC High quality sequence stop: 501 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..547 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5779" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 547 BP; 189 A; 93 C; 124 G; 141 T; 0 other; attggaataa gagaaaatta acattagcca gaattacatc agatattccg atgttacatg 60 aaaattatac cagatctaga caatttcaga tcatgtcctt tgttaatact atcaaggacc 120 taaaaaaaca gaaaaattaa cctatttaca tcaaaggatg taattaacaa catcttatct 180 aatctacacg acaatttaat ggaagattaa tcttccaaaa aaaaaaaaag agaaaaaaaa 240 atctccttag tcctttcctt tctttctgct gtctttttta tgaaaaatta aaagattaaa 300 aaaaaaggtt aatattcatt aggtggagcc aagttaagat cgagattgag ctctcttttg 360 ggctggcaat cgacgaccac ggaggacgag tcggagtcgc tctggaccgg acccggttcg 420 aatcggagtg ggaagggctg ggtcatgaag tgggcccggt cgagaaagta aaccgggcgc 480 atgggtgcga cggggtgggt cacgaggatt tgctgctgga tgggtaggaa cggaaagcga 540 tccattg 547 // ID EH225030; SV 1; linear; mRNA; EST; PLN; 506 BP. XX AC EH225030; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5920.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5920 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-506 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 701651c625aac11b68cc8fcb67747f4f. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: p column: 15 CC High quality sequence stop: 504 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..506 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5920" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 506 BP; 97 A; 160 C; 109 G; 140 T; 0 other; acatcccaca gcttcggcag atcgcttagc cccgttcatc ttcggcgcaa gagcgctcga 60 tcagtgagct attacgcact ctttcaaggg tggctgcttc taggcaaacc tcctggttgt 120 ctctgcaccc ctacctcctt tatcactgag cggtcattta ggggccttag ctggtgatcc 180 gggctgtttc cctctcgacg atgaagctta tcccccatcg tctcactggc cgaccttgac 240 ccttgttatt ttgaggtcat atctagtatt cagagtttgc ctcgatttgg taccgctctc 300 gcggcccgca ccgaaacagt gctttacccc tagatgtcta gtcaactgct gcgcctcaac 360 gcatttcggg gagaaccagc tagctctggg ttcgagtggc atttcacccc taaccacaac 420 tcatccgctg attcttcaac atcagtcggt tcggacctcc acttagtttc acccaagctt 480 catcctggtc atggatagat caccca 506 // ID EH225031; SV 1; linear; mRNA; EST; PLN; 557 BP. XX AC EH225031; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6058.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6058 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-557 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; bca080380e1d3a76a8f16386cb3fa444. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: d column: 4 CC High quality sequence stop: 506 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..557 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6058" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 557 BP; 197 A; 117 C; 78 G; 165 T; 0 other; cacaaaactc tttatttata tgatatcaac ctaatgatat gaaataagat gtccttaaat 60 gcacgcatgg gagataaata taagataaag aagaatttaa aatatgcgtg tcactttaca 120 acatacaaac ctctttagag gagggcatac ataaattgat caacccaact ccgattacaa 180 cacttcgttc tccagtatat atacacacac acatcaacat tcaacaataa acagaacata 240 aattaaatgt tgttgcaatt ggctgttgga ttttgcccat ttgccaacac aaattatgca 300 taaacaagtg ccatgttgca ttaccacctt tttttttttt taccaaaagc gaggtcgaca 360 gcatgacctt aatcgcaatt gcataaaaaa aaatatgaac aatcaaaaag aagcttgtaa 420 ccaaagcttg tactagcatt ctgcatagcg gcccccttcc cccttcaaaa agttgtcaat 480 ttgttctagc gcctcaattg caatatctgt tggagacaat tcggacacat ctcttttgcc 540 cagttttatt gctatat 557 // ID EH225032; SV 1; linear; mRNA; EST; PLN; 710 BP. XX AC EH225032; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5604.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5604 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-710 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 6333bf41314776f0446aef86ebc11921. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: g column: 10 CC High quality sequence stop: 583 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..710 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5604" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 710 BP; 221 A; 138 C; 134 G; 217 T; 0 other; aatattaaac aatttgtaga tgactaattt ggtcttacaa accacttaca attttgacta 60 cgaattcaca aaacggaaac atcattaaag aaactgaaca agaaaagcaa cattcatagc 120 catcaccgag gcaaagatat gaaaacctat atttatttat gctcgatgcc attcgctgga 180 atttctgcag gtttaggaag aagccctttc tctatgcatg tatcaattgc tccagtgtac 240 atatcctcta agctgtattt aaattggaat cccaagtctg tgattttctt ggaagaaaat 300 ctcacgggct ccaattgatc tggaatgttc tgaaacttgg tgggaacctt gtactctggg 360 tatttttcgt taattaattt tacaatgtca tggatagtaa cgtcacatgc actgcatata 420 tacctccctt ctgcttttgg ctgttcaaac agaaatatgt gagcaagaca gatatcttct 480 atgtggacga attgagcttg ctttatgatt gaataatgtg cctcaattcc gttaataggt 540 gaaagagcac tgatcacgct agaaggtatt gttggcagta gaaagggacc aatgacaaga 600 gctggaagga tagcgatgaa gtccatgccg tgctctttcg caaatttcca agcttctttc 660 tccgcaagtg tcttagaaac gaaatacatc caccagtcta tttaatctcc 710 // ID EH225033; SV 1; linear; mRNA; EST; PLN; 679 BP. XX AC EH225033; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5921.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5921 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-679 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 64468bb6f870a94a4136fbc20433bc82. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5921.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: b column: 17 CC High quality sequence stop: 614. XX FH Key Location/Qualifiers FH FT source 1..679 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5921" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 679 BP; 196 A; 133 C; 173 G; 175 T; 2 other; tgaacgtctc aaaggccatc atcgaaggta ctattgatgc tggtatcgga cttgagaata 60 tccaacaggt cgagctggag gagtggtgca aatctaacgg ccgaccagct agtgatgtca 120 aaatgttgag aatcgacgag cttgccgagc ttggttgctg ttgcttctgc tcaattttat 180 atatcgctaa cgaagattgg ttggcagctc atcccaagga agcttccgca tttatgcgtg 240 ctgtaaaggc cggagcggat gatatgtttg ctgatccacg tggcagctgg ttagagtact 300 gcaaatttaa gcctcctatg aacactccct tgaatcgatt gatgtttgag cgtagcttta 360 actacatgag caaggactta gcaaatgtgg ctcgtgactg gaacaaggtg accaactact 420 caaagcgctt gggaattgtt ccaacagact ttgtaagcaa ctacaccaac gagtttgtcc 480 aatgggaggt agctgatgag ggaggtgagg ccgaaggtct ggcaaagcaa caaaagatga 540 aggagattnc agcctatgta gctatccatg gtggtgtgct tcaagctgtt tcagcttaaa 600 aaatcaggaa cttggtgnca cgtgaaatat gtcgcagtgt accatatatc tttttaaaaa 660 aaacaacaac aaaatatcg 679 // ID EH225034; SV 1; linear; mRNA; EST; PLN; 669 BP. XX AC EH225034; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5923.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5923 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-669 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 18663da3ab2fb2df7a44059582517aed. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5923.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: f column: 17 CC High quality sequence stop: 651. XX FH Key Location/Qualifiers FH FT source 1..669 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5923" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 669 BP; 189 A; 127 C; 153 G; 199 T; 1 other; tgtcaggtcg gaacagtgag tagagaataa gctatgcagt tcaatatcac atcacaattc 60 cttcatatct tctctcttac cttcttcctt atcattacct cttcactttc acaatccaac 120 cagttttgtg atgctgggac tgaaattcca tcatccaagt tgttgatcaa gggaggcacg 180 gttgtgaatg gtcaccacca acagatggct gatgccaccg gcaaatatgt cttgccaggg 240 ggcattgatc ctcacaccca tctagatatg gatgttggtt ttactgcaac aatgcatatt 300 gacttcgtta taccacttaa tgggagtttg acagctggtt ttgatgacta tgaaaagaag 360 gcaaagaagt cttgcatgga ttatggtttt catatggcta ttaccaaatg ggatgaaact 420 gtttccagag aaatggagct catggttaag gagaaaggga tcaactcttt taagtttttc 480 atggcataca aaggaattct tatgatcaat gatgagcttc ttctgaaagg atttaaaaaa 540 tgcaagtctc ttggtgccgt agccatggtc catgcggaan atggagatgc tgtgtatgaa 600 gggcaaacag aaatgataga acttggaata ctggtcctga gggcatgctc tttcaggcct 660 gcagtgttg 669 // ID EH225035; SV 1; linear; mRNA; EST; PLN; 544 BP. XX AC EH225035; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5781.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5781 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-544 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 27f35d2c265e8c6cf94b8f5015497fe0. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: i column: 5 CC High quality sequence stop: 314 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..544 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5781" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 544 BP; 192 A; 135 C; 86 G; 131 T; 0 other; gaattgtttt ctacaaatgc attacgagtt cacgtaatta cacattattg tacaaatccc 60 gaattgaaaa ataaaaaaga aacaagaact gttacagaaa tcaaacacag ttggctttga 120 atagtaaaag cagtttttgc atcacacaaa ttcgtactat aaataacaca ttaacactta 180 tcgtaattaa gccccaccag aaccatactt ctttcccagg acaagtacct gaagcttgtc 240 ataaccagaa agacaccagc accagcaact gcacgaagga tgtttgctcc cgcgcccttg 300 aaccaagatt ttgaaccttc attcttcaca atttgcgaga atgcatcgaa agagctcttg 360 tacttgacag cttcaccaga tgtcatcatc attcttctgc gaaccgtatc taagggggac 420 gaggcaatgc tagcaccaat agtaaccatc caccccagag caaagctagc caagaaacta 480 tcctgcaaag ttcctaccaa gagcactggt ttcagagaat catacattcc acagtacaaa 540 ccac 544 // ID EH225036; SV 1; linear; mRNA; EST; PLN; 572 BP. XX AC EH225036; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6066.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6066 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-572 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 84650a0e6b0103f8e4234556e4e364d8. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6066.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: d column: 6 CC High quality sequence stop: 482 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..572 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6066" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 572 BP; 203 A; 138 C; 93 G; 138 T; 0 other; ataaaccata aacagtttat aaaacttcgt tataaattaa atgcaactta aaacatccct 60 cagcagcaaa ggaaatggac taaaacccta tagctgcttg tgaaattcga aatatctcag 120 actcttctta ccatcaaaac aaaaaacagt actcatcaaa aacaaacagc tatgaaaatt 180 gaccaccgcc gatcaagaac ctagttttga aagttacggc tacatgaaat cggcagaaac 240 ttaaaaatat aacttaaaca aaacccctaa gactctgcaa ggataaaact actataaagg 300 agtaaccacg atgatgatca catgtgctag caaaattgcc caaatgcaat cacttcttct 360 tggcagcggc cttggtgacc ttggctccgg tggggtcttt cttctcaaca ctcttgatga 420 ctccgacagc cacagtttga cgcatgtccc tcacagcaaa acgaccaagg ggaggatact 480 cagagaaagt ttcaaccacc atgggcttgg ttggaatcat cttaaccata cctgcatcac 540 cattcttcaa aaatttgggc tccttctcaa gc 572 // ID EH225037; SV 1; linear; mRNA; EST; PLN; 438 BP. XX AC EH225037; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5797.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5797 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-438 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e2a612ebd5ff723f6aa20190ec440731. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5797.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: i column: 9 CC High quality sequence stop: 438 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..438 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5797" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 438 BP; 136 A; 68 C; 104 G; 130 T; 0 other; ccatcccgta accatgtctc agactgttgt cctcaaagtt ggtatgtcat gtgaaggatg 60 tgttggagca gtgaagagag ttctaggaaa attggatggt gtggaatcat atgacattga 120 tttgaaggaa caaaaggtgg tagtgaaagg aaatgtccag ccagacacag ttctgcagac 180 cgtttccaaa actgggaaga agactacctt ctgggaaggt gaagcagcaa catctgaaac 240 tagcacagca actgcctaaa acatttgcct ctattctgtg cagaagaagt agctgtgtga 300 atcaaatact atttgatggt cttgaaataa tgatgaaact ttgttatctg gtgtatttta 360 gtgtcaaaaa aaagactgat gtatattact gcttttcacg tcagtgatac tgtattaatg 420 tgcttttatt gagagttt 438 // ID EH225038; SV 1; linear; mRNA; EST; PLN; 642 BP. XX AC EH225038; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5925.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5925 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-642 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 297e7311e42bd32fc134d5fd459c6d52. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: j column: 17 CC High quality sequence stop: 534. XX FH Key Location/Qualifiers FH FT source 1..642 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5925" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 642 BP; 137 A; 186 C; 191 G; 125 T; 3 other; ctcacaccag gcgcacgtta gcacaaacta tcatggccac ggcaacagca gctgccacct 60 cgtcctttat ggggacgcgt ctcctggagg ctcactccgg ggcggggcga gtgcacgccc 120 gattcggctt cggcaagaaa aaggctcccg ccccaaagaa agcctccagg ggatcgggcc 180 gagacaccga cagacccctt tggtatccgg gcgccaaagc gcccgaatat ctcgatggga 240 gtcttgtcgg agactacggg ttcgatccgt ttgggctagg gaagcccgcg gagtacctgc 300 agttcgagct ggactcgctg gaccagaacc ttgcgaagaa cgtggctggg gacatcattg 360 gaaccaggac cgagctcgcg gacgtgaagt ccacgccgtt tcagccctac agcgaggtgt 420 ttgggctcca gaggttccgt gagtgcgaac tcatccatgg aaggtgggcc atgctcgcca 480 ctctcggagc tctcactgtt gagtggctca ctggtgttac atggcaagac gccgganagg 540 tggagctagt agaagggtca tcataccttg ngcaaccact tccattctnc atcaccacac 600 tgatctggat cgaggttctc gtaattggct acatagagtt cc 642 // ID EH225039; SV 1; linear; mRNA; EST; PLN; 702 BP. XX AC EH225039; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5610.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5610 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-702 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ca6479ef8c82cf6540210f8e98229722. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5610.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: c column: 12 CC High quality sequence stop: 664. XX FH Key Location/Qualifiers FH FT source 1..702 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5610" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 702 BP; 181 A; 140 C; 174 G; 207 T; 0 other; cttggatcaa aaagggttgg attgattggt cttggtcagt caccttccac tcctgcacct 60 tttaagagtc ttggcgaggc cattgtggcg gctgccaagt cttctcaagc aagcagtgtt 120 gctgttgctc ttgcctcttc tgaaggactc tccgcacaat caaagcttag cactgcttat 180 gcaattgctt ctggggctgt gctgggatta tttgaagata atagatacaa atcagaagca 240 aagaaatcaa cactaaggtc cattgacttt attggtcttg ggacaggacc tgaactggag 300 aagaaactga agtacgcagg agatgtttct tccggaatta tctttggaag ggagcttgta 360 aattctcctg ctaatgtgct cacaccaggg gtgttggcag aagaggcagc taagattgct 420 tccacctaca gtgatgtttt cactgctaaa atattaaatt ctgagcaatg tgcagaattg 480 aaaatggggt cctatctggg tgttgctgca gcctcggcta atcctcctca ttttatccat 540 ctatgttaca aacctctgag tggacctgtc aatgtcaagt tggcattagt tggaaaaggt 600 ttgacttttg acagtggtgg ctacaacatc aagactggac ctggctgttc aattgagctc 660 atgaaatttg atatgggtgg ttcagcagca gtttaggaac ag 702 // ID EH225040; SV 1; linear; mRNA; EST; PLN; 621 BP. XX AC EH225040; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5785.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5785 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-621 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; cd1484b8bc2256a23ae58eb40182ce19. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5785.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: a column: 7 CC High quality sequence stop: 598. XX FH Key Location/Qualifiers FH FT source 1..621 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5785" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 621 BP; 127 A; 197 C; 169 G; 127 T; 1 other; ctaaaacatg gtccaagaaa agaaactagt tgacgaggtg tccggttggc tcaaaattta 60 tgacgacggt tcagtggacc ggacatggtc cggaccggac caattcaagt tcatggccga 120 accggcccct ccccatgaac aattcataga cggcgttgcc attcgcgacg tcgccgttac 180 tcacggtggt ggccagagtg gccaccatgt ccgactttat ctgccagaga taaagccgga 240 agacagccaa aaactaccaa ttgtcctcca tttccacggc ggcgggtttt gcataagtga 300 accggactgg ttcatgtact accaagtcta cacccggttc gcccggtcca ctcggtccat 360 cgtggtttcc ccgttcctcc gccgtgcccc agagcaccgc ctccccgccg ccatcgatga 420 cggctttgac accctcctct ggctccaaac cgtggcccgg tccggttcac tcgagccgtg 480 gctcgagcaa cacggtgact tcaaccgggt tttcctcata ggagacagtt ctggcggaaa 540 ctcggtgcat gaggtggccg cgagggccgg ttcggcggac ctaagcccgg tccgggtcgc 600 gngggcaatt ccggttcacc c 621 // ID EH225041; SV 1; linear; mRNA; EST; PLN; 323 BP. XX AC EH225041; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5609.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5609 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-323 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 7d8c9fe0cafcef3ddc6f531b1a831fbb. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5609.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: a column: 12 CC High quality sequence stop: 311. XX FH Key Location/Qualifiers FH FT source 1..323 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5609" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 323 BP; 97 A; 117 C; 40 G; 68 T; 1 other; aagaatctag aaaccagaat accattggac ctcgtttctc caacgtggct ttttccaaac 60 gctaaattgc tactccaaca ctctgagcca cgttccccat ctctgccact gccatcagca 120 ttctcaacac ctcctccctc actcactcat caatggcatc aacaatatca gcaatggcca 180 tgatcaacgc caaatgcgtc aacccaaagt tccccaaccc caccaagctc gtaccatcaa 240 aaccttccct tcaggtcaac cttccaaagg gcctaatagc ctcacccgaa aacaccaaac 300 tttccactgc gaacaggccn aat 323 // ID EH225042; SV 1; linear; mRNA; EST; PLN; 610 BP. XX AC EH225042; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5613.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5613 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-610 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 31329b4ce3d97613527d2eb42740b561. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: i column: 12 CC High quality sequence stop: 471 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..610 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5613" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 610 BP; 189 A; 166 C; 78 G; 177 T; 0 other; gcaattaaaa ttcaaccttc tatacaaata ttatcacaac cttcaaaaga ggatcaaaca 60 agtctaaata aagaagttga atttaacaag cattgtgtaa agcacctacc acaaatcagc 120 cagtacaaaa tcaaatatga agccagatgc tttcttttgt gaagatcacc attgttctct 180 tggacgacca tcagggagta caggaagaag gaccatttca tcattctgtc tcgagtcttg 240 tttaatagtc tttccttgcc ccatctccaa aagttcagca tcctcatcaa aatctggcgg 300 caaatcatca tcttcttcat cactccagtc atcaccatca tcaatgtcat caaacccatc 360 atcattcatt aaattatcaa catcttctaa ccattcttct tcctcttcac cactttcatc 420 ttctctgcta tcacttcctc tcatgctatc ttcaactatc ctgcgagcag gtcccttggg 480 aaaccgtgga acattaacca gagtacgaag cttttccttt ataagcaaca accggtcctt 540 ctccaccaac tgagaatctt ggtagccttc cctcagaaac acagaatccc tatctccttt 600 aagagagacc 610 // ID EH225043; SV 1; linear; mRNA; EST; PLN; 367 BP. XX AC EH225043; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6076.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6076 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-367 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ab138a6c124a84e482f22b325218012f. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: h column: 8 CC High quality sequence stop: 367 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..367 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6076" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 367 BP; 117 A; 81 C; 83 G; 86 T; 0 other; accacaacag agaatattaa ataagaaacg tccaaagtgg ccttattcag gggtaatatg 60 aacattaaag tggattcgtc gtggcataaa tacgatgatg taaaagtaat actatccttg 120 taatattaca ttacacacaa tctgcactta gtggacactg gatggatgct atatcattac 180 ataacacacg aaggctgcga ataagtagct aatacccttt tcacgaagaa gagtgctggc 240 cctcgcacgc ggggctatcg tcgaaattct tcgatggata cttcgccgtg accataccga 300 cctcgaagac gatatcatcg ccggaggagc ggattccggt gatggaccac cacttgaaga 360 acgcgcg 367 // ID EH225044; SV 1; linear; mRNA; EST; PLN; 571 BP. XX AC EH225044; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5793.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5793 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-571 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 57c546e0101b61ea5e3e6829b63d5ebf. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5793.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: a column: 9 CC High quality sequence stop: 570 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..571 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5793" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 571 BP; 191 A; 125 C; 117 G; 137 T; 1 other; ctccccgcac ttcagcaata gaaatacatc tcagatggca caaattaacg cataatggca 60 ttattaggtc attgacaaat ctgtatcaat gaataagata ttcatcccaa actgcatgca 120 aagtcaggtc aagctggctc ttctaagaga atctccgaac agctcattcc caggcaaaga 180 cgggagaaac tgtcaattta gaatagatga acgtatctgg aaaccttcat caccttcaga 240 aagcagcggg aatcttgtca agaaattgcc acaggtttgt tcccaggtgg gagtttactt 300 agggtatcag tgtcagatac tttggatgga ggccagtagc gaaacatgga cctaccaaca 360 atgttctcaa ctgggagagg gcccataagc tagaagtttt gaggaaacag catcaacaaa 420 tgggccaaac tttgtcatcg acctaggcta aaatatcctc caacataaac agagaaaagt 480 cagcttgaaa gggactgana aattaccagt tatgagaatc aaagctgttg ttccgattgt 540 ctcccattac aaacacatat ccttctggca c 571 // ID EH225045; SV 1; linear; mRNA; EST; PLN; 317 BP. XX AC EH225045; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5937.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5937 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-317 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3192e8b9cf46489acfc9328b5be83407. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5937.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: b column: 21 CC High quality sequence stop: 317 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..317 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5937" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 317 BP; 73 A; 54 C; 60 G; 130 T; 0 other; ttgaagttcc agtttatgtg gtgttgtctt ggtttacttt ggcagttctt cagagttatg 60 aagcctttgt cctcaggggc gttggtcatg gcagcaagtt atcctggtct gtcatcacaa 120 tcttttcccg ttgggatggg ttgtttttta ctaccaaagc cctagctttt taagcttcat 180 tttttttttt tgaacttgtc attgtaacct tctatatcat gtaatgatac tgagatatgc 240 cctttgtact gttatgttta ttttatgaaa aaataataaa taaaaaattg tagtcccctg 300 tccaaaaatt tgccttg 317 // ID EH225046; SV 1; linear; mRNA; EST; PLN; 424 BP. XX AC EH225046; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5789.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5789 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-424 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 462cbc5ca8720a2d1f5779037fa2d41d. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5789.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: i column: 7 CC High quality sequence stop: 377. XX FH Key Location/Qualifiers FH FT source 1..424 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5789" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 424 BP; 94 A; 115 C; 108 G; 107 T; 0 other; ccaatgctac ctgtgagaag cgtttggctt ccattgggct agagaacact gaagctaacc 60 gccaggcata ccgtaccctc ctcgtgacag ttccaggcca ttggtcagta catctctggt 120 gccattctct ttgaggaaac actctaccaa tccacaactg atggcaggaa gattgttgac 180 gtgctgcttg agcaaaacat tgttcctggt attaaagtcg acaagggttt ggtgcccctt 240 gcaagttcca acgacgagtc atgctgccaa ggtttggatg gtctagcctc tcgctcagct 300 gcatactacg agcaaggtgc ccgtttcgcc aaatggcgta ctgttgtgag catccccaac 360 ggtccctctg ctctggcaga taaggaagca gcctggggtc tggctcgcta tgctgcaatt 420 gctc 424 // ID EH225047; SV 1; linear; mRNA; EST; PLN; 371 BP. XX AC EH225047; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5636.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5636 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-371 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 27031da23e4e017568f3ba6d87df754b. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5636.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: g column: 18 CC High quality sequence stop: 371 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..371 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5636" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 371 BP; 93 A; 60 C; 75 G; 143 T; 0 other; agcatcgtca aggtcctcgt ctgtgttcat acctcacggt cctggggcag ttaaagacat 60 tgccgtgcaa attcgggatg gtctccttca ggccactgcc tcacagaatt aagctttctt 120 ggagttgtaa ataacaaata gtatgtgtgt caggtttttt tcaggaaatg cttcttttgt 180 ttgaacttat aaagtctgca tctttactct tcttatattt gatagattca tagcagtgtc 240 tattttatgt gtaagtactg atgtgcagtt ttgaatactt gtttaaggat agtcttaatg 300 tcaagttgct tgtgaatcat taaggaattt gttatgtaag aattacttcc caaaagatgt 360 gtttttcttt t 371 // ID EH225048; SV 1; linear; mRNA; EST; PLN; 511 BP. XX AC EH225048; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6086.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6086 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-511 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 579ae47c60600fbeacf71a33a1955e76. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6086.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: l column: 10 CC High quality sequence stop: 468. XX FH Key Location/Qualifiers FH FT source 1..511 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6086" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 511 BP; 169 A; 123 C; 134 G; 85 T; 0 other; ttaacacttt ccaatacaat aatcactaac tgaatattcg atcggagatg tctgccgccg 60 ccgaagaagc taacgctccc gccgtcgaaa agccggttga ggaggtgaag gcgccgaacc 120 ccgcgaagga taagaaacct aaggctccga aggaaaagaa gcctaaacag gccaaaacag 180 cttcacatcc tccatatttt cagatgatta aggaggcttt gatcgctttg aacgagaaag 240 gaggatcgag tccttatgcg atagcgaagt acatggagga gaagcacaag gcggtgctcc 300 ctgcgaattt caagaagatt ctaggtctgc tactaaagaa ccgagctgcg agagggaaac 360 tcgtgaagat caaggcttcg tacaagttaa ccgaagccgc gaagaaggag aacaccgccg 420 aagtgacgaa tgccaacgct gagaaaaagg aatcgcgtcc aaaacgaagc ataaccgcaa 480 ccgccgctgc tccgaagaaa accgaggcgg t 511 // ID EH225049; SV 1; linear; mRNA; EST; PLN; 499 BP. XX AC EH225049; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5934.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5934 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-499 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e3fb3f740793883845a8d65ea4500134. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5934.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: l column: 19 CC High quality sequence stop: 499 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..499 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5934" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 499 BP; 172 A; 118 C; 80 G; 129 T; 0 other; aaacagtaag aaaagcatca ccaaactaat gaattaaggt gcaacaattt cacttgtttt 60 cacaaatatg acaaaagtaa taaactcact atttgtcaca aatatgccaa atcactataa 120 caaaataaaa aatccaagat atagatagaa ttagaacagc aatggataag attacatcat 180 atatatatat attgatcatt cgatctctcc ctccttgtgt gtctcaatga caacgtctga 240 cctaggttga gcaacacagg tgagaaccca gccactatct atttgctcat catcaaggaa 300 gctaccatct gactggtcca cgttaccttc cacaattttg ccaacacagg aaacgcaaga 360 accggccctg cacgagtagg gaagttcaat gccttcgtcc tctgccttgt caacaatgaa 420 aacatcatct gggcattcaa actccacttc tccttctggg gttatgagct tcaccttgta 480 cgaggccatg caagtgaca 499 // ID EH225050; SV 1; linear; mRNA; EST; PLN; 456 BP. XX AC EH225050; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5620.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5620 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-456 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 5425e7dd2fa3f617659f34af866b29a5. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5620.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: g column: 14 CC High quality sequence stop: 358 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..456 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5620" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 456 BP; 133 A; 93 C; 86 G; 144 T; 0 other; ttccaagtta acatggaggg attggaaata aactattaga gccttgtact taattcatgt 60 aagcatgact agcatgtttt tcaactcaga ctgagactga ggcatttttt caacttgaca 120 tcatcccagt cagaagcttg gttatgctgc ttgcaaccag aggcagacga taaattcatt 180 tatttattag atatattgac atcacatata ggtgccacac gtttagattt gtgacaagcg 240 tacatttctg atactgcttt cttccctaat aaagaaggct tctttgcagt ctgaggagta 300 gtgacgaaat ctaccattct tcagcatcca aaatcataag gaaccaatcc tttcagctct 360 gggccgctga gtttgttatt ctcaaaactt tccatcggga aggattccaa gttgccatct 420 actggtaatt gttccacaga ggtccttatt agaaac 456 // ID EH225051; SV 1; linear; mRNA; EST; PLN; 281 BP. XX AC EH225051; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5804.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5804 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-281 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 399f43e74fac74899c24de7a8677b06e. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5804.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: g column: 11 CC High quality sequence stop: 281 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..281 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5804" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 281 BP; 81 A; 49 C; 45 G; 106 T; 0 other; ctctttcttt aaacacgtat acgcatgcac aaccccctta tgattccttg caatcagtac 60 ccttcactaa cagccttcaa agtgttggac gctaagttgc taagtcaagc ttcaaattta 120 tctatttcgg gcactatgaa gttgtgcaat ctatgtagaa tttcattttt tttttatgat 180 tttaatttga tgacctttgt gacaaaatgt ttggtgactt aacagatatg taaaatggtt 240 ggaagagagt ttccttttga ataaataata atgcttattt c 281 // ID EH225052; SV 1; linear; mRNA; EST; PLN; 597 BP. XX AC EH225052; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5801.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5801 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-597 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 233490d908b88a5e7226501255ee4975. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: a column: 11 CC High quality sequence stop: 534. XX FH Key Location/Qualifiers FH FT source 1..597 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5801" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 597 BP; 153 A; 122 C; 126 G; 196 T; 0 other; cacaaacacg tccacttttt ctgctccaat tgacaaagaa cttaaaaagg ttgctcagaa 60 gactgctgct acctttgcac caagggcttc cacggctaca aaaaaccctg cagtgcctgg 120 aactgttttg tacagtgttt ttgaggttca agggtatgtt tcaatgttgt tgggaggagc 180 tttgtctttt aatctcatat tcccttctga tgaacctgac atttggagat taatgagaat 240 gtggtccatt tggatgttca caattccttc attacgagct cgagattgct caaagaatga 300 gaaagaagct ctcaactatc tttttctcct cgtcccattg atcaatgtta taatcccatt 360 cttttggaag tcctttgctg ttgtttggtc cgcagatgta gtagccttct tgggaatgta 420 cgcatggaag tttggatggc ttcagagaac ggattaagga acccaaattt ggttagtaaa 480 agttatcaat gcttgttggc tttgtctttc tcatggatct caattttgta taacacgcag 540 caatgagatg tgacattcct tgtagcactt catggccccc aactcatgta tgagcga 597 // ID EH225053; SV 1; linear; mRNA; EST; PLN; 553 BP. XX AC EH225053; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5805.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5805 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-553 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 1e02ef0d1cd71ed2fdecae28b0714015. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5805.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: i column: 11 CC High quality sequence stop: 467 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..553 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5805" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 553 BP; 185 A; 111 C; 85 G; 172 T; 0 other; gatgtacatg acaagtaata ttataaattc acttgtacac ggcttatagg aaaaacaaac 60 aaacaaatac aaattaataa gtattcaaac cataaactta accatctatg ataggctctt 120 gccacctacc atcactattt tttttatatc agctataaca tgcttagttg agatacaaat 180 ataataacgc aaaatatact ttaatactat atgcttcaga ttatgtaata ctgacacgtg 240 tcacaaggac aattggtcaa cgcactccag tctgaaagaa gaaatgaatg attttggaat 300 ttacatgttt ttattacatc ttgggtgctt cctttgagtg aacagaacga gtattcagta 360 gaggctgtag agctttcact ataatgctca tatttggtcg aaactcagct tcatattgca 420 cacacaatgc agcaacagca gccatctttg caactgattt tgaagggtac tctcccttta 480 gtctaacatc aacacactgc ttcaccttat cttcactaag ctttggtgtt gcccaagtca 540 caaggctttg ctg 553 // ID EH225054; SV 1; linear; mRNA; EST; PLN; 640 BP. XX AC EH225054; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5813.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5813 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-640 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 199e7b10962c77016059a1fc352197d1. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: i column: 13 CC High quality sequence stop: 584. XX FH Key Location/Qualifiers FH FT source 1..640 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5813" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 640 BP; 141 A; 179 C; 140 G; 180 T; 0 other; cacacctcta ataactcaca ccgggagagc tctatcgatt ttgaggacct accactctac 60 tcaactacct ttaaattaca tccactactg cttaacaatc atcagcgagc ccagctcttc 120 gttcttcctt actccggttc gcccaactta ttgtgtgacc cacttgctcg tcttttgatc 180 cttcatccag atcttggaaa ttgcttcacc tcttctgcct tatcgttaaa ttgctcataa 240 tgggtaagac tacgctgttc gttgctgggt ttggaaccct aagggccaag gagctcgcct 300 atgagtttga acgctatggc cgtctggtga ggtgcgatat tccagctgca aagagttcaa 360 ctgctaaatc gtatgcgttt gtggaatttg aagacgagcg tgatgctgcc gatgcatact 420 atgagatgca tggccgccgc cttgatggag acactttaaa tgttcaatgg gcaaaaaatg 480 ccccgtcatc ttcatggcgt tacgagcgtc gtgagcgatc cccagcttat tctcgccgct 540 cctacagatc gccttctccg taccgtggcc ggagccgtcg ctcccctagt ccgtatcatg 600 gaaggtcctc tagaagaagc aggagtccac ttccttctcg 640 // ID EH225055; SV 1; linear; mRNA; EST; PLN; 583 BP. XX AC EH225055; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5936.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5936 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-583 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 12e9002b5b9a245f3c76672a0ef8b7b7. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: p column: 19 CC High quality sequence stop: 534. XX FH Key Location/Qualifiers FH FT source 1..583 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5936" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 583 BP; 172 A; 101 C; 123 G; 187 T; 0 other; cccatccccg tttgtggatc cttgtctcaa tacgggatga aacacatgag gtcctttgca 60 aacatctgca atgtagggat aaagaatgaa caaatggctg aggcttcagc acaagcttgt 120 gtcagtattc cttccaatcc ctggagttct ctgcaaaggg gtttcagtgc ataataactc 180 cctgtaatgt gcactagtaa agaccaaagt atgattattg ttacattatg ttacatggtt 240 gtacttgtat atacatatct tgtcccacct ttgtaaatac aattgggaca ctactaggat 300 tgggaagaag ggtctttaca tttatagttt ggcaaataga tattgcaact acctttgtat 360 aattctattt ctgaagaagc aattacaatt tacaagggat ggtgccattt acggcataag 420 gattaaggag ggataaaggg accaattgct ttggaatatc cactcattac aatgcatgta 480 tgacaacaca tagtaatatg atgtgtgttt ttattcagtg ggcaactggc agatcgggtt 540 ttccctggtc acttttgtat aattattcgg aagatttatg atg 583 // ID EH225056; SV 1; linear; mRNA; EST; PLN; 480 BP. XX AC EH225056; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5624.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5624 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-480 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 2da7abc6dfe122da073eecbf796c6b31. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5624.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: o column: 14 CC High quality sequence stop: 480 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..480 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5624" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 480 BP; 147 A; 114 C; 85 G; 134 T; 0 other; ttcagattaa ctagccgaac tttatacaga agcgaccaat taccaaacat ttatatatat 60 atatatatat aaaaaaaagg caaccatgac cttaacacac ccatcacaag tccaaaacac 120 ttgaacttgc tcaagggcaa gaaatgacca ctttattcta agggtgtttg tttagacaca 180 aaacaccaga gtgcaacata atacatacta cataattttg aagttttgaa actacatata 240 gtttttatca ttatttctaa gggccatttc tgcttgaagc tttagctctc caacacctca 300 cttgcaagtg caggggttgc aggtgcagtt agctccacat ttgcagccat cgttctcagc 360 gggaacaccc atttcagcac tctcgaattg agccttaact ggtgccactc ccatgaccaa 420 ggtctcggtg gtggttgact cggtgtagct caagtctggg tacatcttgc agcctccgca 480 // ID EH225057; SV 1; linear; mRNA; EST; PLN; 633 BP. XX AC EH225057; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5933.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5933 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-633 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 69746c2ca33715641855a7af5a9baddf. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5933.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: j column: 19 CC High quality sequence stop: 617. XX FH Key Location/Qualifiers FH FT source 1..633 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5933" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 633 BP; 174 A; 131 C; 163 G; 165 T; 0 other; tttgttccat tgtctcaaat atcaacgaac tcaaatgtag aagagcttct tgataaggag 60 cttcctctta agtttgtgga ggtggatgag gaacaatcta gacttgtcct cagcaaccgc 120 aaggccgtag ctggcaacca ggcacagcta ggaattggat cagtggtcac tggctctgtt 180 caaagcctga agccatatgg tgctttcatt gacattggtg gaattaatgg cctccttcac 240 gttagtcaga tcagtcatga ccgtgtaact gatatttcaa ctgtgcttca acctggtgac 300 atattaaagg tgatgatctt aagccatgac cgagagagag gtcgagtaag tctttccact 360 aagaagttag agcccacacc tggagacatg attcgcaatc caaagcttgt ctttgagaag 420 gcggaggaga tggctcagac attcagacag agaatagccc aagcagaagc tatggctcgt 480 gcagacatgc taaggttcca gcctgagagt ggattgacta tcagcggtga tgggatcctg 540 ggaccattta cttcagactt gcctgaagag ggactggatt tgagtgaggt accaccagct 600 gaagaatcat gattcagaat tgcgtgctct ttt 633 // ID EH225058; SV 1; linear; mRNA; EST; PLN; 572 BP. XX AC EH225058; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5632.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5632 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-572 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b12cc73c9567d0e955b616212e594d13. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: o column: 16 CC High quality sequence stop: 570. XX FH Key Location/Qualifiers FH FT source 1..572 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5632" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 572 BP; 167 A; 114 C; 138 G; 153 T; 0 other; gatcctttcg ttcctgtgga tcaaatacaa agtcccttcc cacaccactg taacatctac 60 ttacagaagc acaccacagg aactagaaaa tccatacaat cttcaccacc aaaaagaaaa 120 taatggcaat ggccagtgca acaactctag ccacaatcac tgttgtggcg gctagcccca 180 gtgttggcag cagaaggagt gggaagagga atgtgaactt catcagaggg ttgaattctt 240 ttggagggtt gaaggctcag aacaatgtga cattactagg ccttcctgtg tgcactgagc 300 agtgctttgc aaaggtggtg agctcattga aatatccatc atcatcatca cgtaagggga 360 gaggtggagg tgctgccttt tcaacatgca atgctgctgg tgagattttc cagattgcag 420 ccatcatgaa tggccttgtg cttgttgggg tggcaatagg gtttgtcctt cttcgagtcg 480 aagcgtttgt ggaagagtct gagtgagaat tgcatcaact tttaatcagt ttttcatata 540 tgtattttta taaagaagaa aaaaagcgaa aa 572 // ID EH225059; SV 1; linear; mRNA; EST; PLN; 611 BP. XX AC EH225059; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5814.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5814 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-611 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 124e5dd4e116756f61f0e9c919f5b05e. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5814.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: k column: 13 CC High quality sequence stop: 597. XX FH Key Location/Qualifiers FH FT source 1..611 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5814" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 611 BP; 130 A; 150 C; 168 G; 162 T; 1 other; agcctcccct cccctctctc taagatcccc aaattgcttg tgaatgctag aaaccctaga 60 cagattcgat tggatgcgct cccatttcac atcaagggcg agcgcattcg gttcttgcaa 120 gcaagcctgg tactaccgct gcctcggcct cgacccctga atttgaacca ttcatcacca 180 cctttcttca ttcattcacg gttttccttt tcgactcgat ttgttgtttt ttttgttttg 240 tttccttgac gatgttgacg atgagcaagc agaagaagaa ccagaacgcg aagaggctgt 300 tgataagcat caatgtgctg gggagcgcgg ggcccattag gttcgtggtg aacgaggagg 360 agcttgtggc ggcggtcatc gacaccgcac ttaagtccta cgcgcgtgag ggcaggcttc 420 ccgtcctggg aaacaacacc accggtttcg tgctttattg cccccatctt ggatctgatg 480 ctttgagtcc ttgngagaga attggatcac atggggtgag gaatttcgtg ctgtttaaga 540 agccagaggg tgtggcggat gtagatggaa gtacagcact ttctcgcagc agcaggggga 600 gtgggagctg g 611 // ID EH225060; SV 1; linear; mRNA; EST; PLN; 492 BP. XX AC EH225060; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5644.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5644 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-492 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 2584aab9b775dca766f6687f4cdfd9f3. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5644.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: g column: 20 CC High quality sequence stop: 456. XX FH Key Location/Qualifiers FH FT source 1..492 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5644" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 492 BP; 131 A; 86 C; 130 G; 145 T; 0 other; cgtcggcttt tgagcttggg aggccatcga atcgattcgg cgcggaacaa ggatgatgtg 60 gcggaggcgc tgtcgaggca gtactgggaa tataatgtgc ttgactatga ggagaaagtg 120 gtagatggtt tttatgatgt atatgggccc tataatgatt cagtaatgca aggaaagatg 180 ccatctcgaa cagatcttga agcaaaccct ggaggttctg agttggtcat tgttaatcga 240 acaattgatc cttctctgga ggaactgata caaattgcac aatgcattgc attagactgc 300 cctgtctcta gtttggtaca aaggcttgct gaacttgtta caagtcatat gggtggtcct 360 gtaaaggatg ctagtattat gttagcaaga tggacagaaa caagagcaga gttaaagaca 420 tctcttcaca caattgtatt gcctcttggg tccttaaata ttgggctctc taggcatcgt 480 gcttttgctt tt 492 // ID EH225061; SV 1; linear; mRNA; EST; PLN; 556 BP. XX AC EH225061; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5653.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5653 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-556 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; dc958a7cab2656e332ca6336a60a68ad. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5653.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: i column: 22 CC High quality sequence stop: 554 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..556 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5653" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 556 BP; 165 A; 129 C; 99 G; 163 T; 0 other; tttttttttt tttttttttg aacatgacaa tttcatatat tattattata cttcacaatc 60 agcatttctt tgttcaagac tgataataac cattaaatta ttgtcattgt aacaatcgga 120 aaacgaggga aaggaataga agaaaacaat gaacaaccct tgccagttag ttgcaccaac 180 caacaaggca ccaaccatag taccaatcaa aaggtaaaaa gagagagaga ccaaatccta 240 ctaagaaaag cataactgtt cctttccatc caaagggaga aaacatgcag gttttgctca 300 tggcttccac agaatggttg tgtctgcaat catggaagta accacgtatg ggtccatgtt 360 ggaagctggc cttctgtcct caaaatatcc cttccctgct ttctctgtgt ctctcccaac 420 cctaacagaa gctccacggt ttgcaactcc ccataagaag gtgttgatgt cagcggtttc 480 gtggcgtcct gtcaaacgac gttcgttgcc ttctccataa gcagcaatgt gctccttgtg 540 cttcttcccc aacttg 556 // ID EH225062; SV 1; linear; mRNA; EST; PLN; 329 BP. XX AC EH225062; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5641.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5641 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-329 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ed4e915f3b6707cbb679001b3a58c888. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: a column: 20 CC High quality sequence stop: 287 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..329 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5641" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 329 BP; 85 A; 57 C; 49 G; 138 T; 0 other; gcaatggtca tcatgtatat tgccaaagtc tcataaaatt gtatgttcct tcttgactca 60 gtaagttaga aacataaaga atgtcttgat ctaacaattg ttaggttgtc tggcaaattt 120 cttctatttt ttttattttt tttccaaagt taaataaaag tcttatgaag caatgtaaaa 180 cactgctata gccgttttga ggctttcctt cttactatcc ttttcttctt ttatgactta 240 aaatgaaatc atcatcgtcg tcgctctcta caattctttt cttggctttt atgtcaccat 300 ttttggatgt ctgttgttgc ttctgggaa 329 // ID EH225063; SV 1; linear; mRNA; EST; PLN; 619 BP. XX AC EH225063; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5639.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5639 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-619 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3557be779159de9bddac607c75439150. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5639.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: m column: 18 CC High quality sequence stop: 501 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..619 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5639" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 619 BP; 203 A; 133 C; 102 G; 180 T; 1 other; gtgaacaaaa gatgaatcct tttcctagat gtctaaaaat gcattctatg ttcattaaaa 60 aaacaaaaag tgtcatgcta catcctgcaa cattcaatta aataaatgca ctaacatatc 120 aaattgaata aagaatcatg aaaaacaaaa aatcaaaacc ctacacaact atagttccag 180 aagtggctgt ctcaaactat gaagctaatt cccaatgggg ggcacaacaa tgatgagagg 240 aagcaataag aattgattca tgatagctcc agccaggtct gtacatgaca ttttagtttg 300 agaagtgtag aactcatgcc acttccatcc attcatgaaa atacatatgt aaccatcgct 360 cactttcaat ttgaatctat ggactctcgt cgtctgaact aatgtcatcc atcctccttt 420 cagcaattgc tggaaaaagc cgntgatgaa aatcaggtgg tggacgtgtt tcagatgaag 480 atgctcctct aggagtctct tgttttagag tttttgccag cttcttccgt gtatctctca 540 cctccttgga tacctttatc caccattatt aagaagtctc gtactactat gaacaagctt 600 atgccggtca tccttccca 619 // ID EH225064; SV 1; linear; mRNA; EST; PLN; 615 BP. XX AC EH225064; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5637.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5637 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-615 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 2b97ce0e4948575486c0f330f7a8b70a. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: i column: 18 CC High quality sequence stop: 566 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..615 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5637" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 615 BP; 192 A; 165 C; 78 G; 180 T; 0 other; tgcaattaaa attcaacctt ctatacaaat attatcacaa ccttcaaaag aggatcaaac 60 aagtctaaat aaagaagttg aatttaacaa gcattgtgta aagcacctac cacaaatcag 120 ccagtacaaa atcaaatatg aagccagatg ctttcttttg tgaagatcac cattgttctc 180 ttggacgacc atcagggagt acaggaagaa ggaccatttc atcattctgt ctcgagtctt 240 gtttaatagt ctttccttgc cccatctcca aaagttcagc atcctcatca aaatctggcg 300 gcaaatcatc atcttcttca tcactccagt catcaccatc atcaatgtca tcaaacccat 360 catcattcat taaattatca acatcttcta accattcttc ttcctcttca ccactttcat 420 cttctctgct atcacttcct ctcatgctat cttcaactat cctgcgagca ggtcccttgg 480 gaaaccgtgg aacattaacc agagtacgaa gcttttcctt tataagcaac aaccggtcct 540 tctccaccaa ctgagaatct tggtagcctt ccctcagaaa cacagaatcc ctatctcctt 600 taagagagac ttaaa 615 // ID EH225065; SV 1; linear; mRNA; EST; PLN; 356 BP. XX AC EH225065; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5814.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5814 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-356 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ae7ba073ec9e395d3be87dfbb0b70aa6. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5814.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: k column: 13 CC High quality sequence stop: 160 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..356 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5814" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 356 BP; 121 A; 67 C; 86 G; 82 T; 0 other; gggggaaaaa aaaatttttg gtttccccca aaaaaaaaac ccaaagggga aaggtgaaaa 60 atttcccaaa aaaaggggtt tctgggtggg gggaaaaaaa aaaacccaaa aaaaaacccc 120 ctttttgggg aatttttagg ggggttcccc caaaaagggg ttttggttaa ttcccccaaa 180 aaattttttt tttgggggga aaaaacttgg gggggggggg atttgtgaaa aaaagctttc 240 aactcccacc ccccctgggg gggggaaaaa aaggtgtatt ttcatttaaa accgccaaac 300 cctttgggtt tttaaaaaag aaaaaattcc ccccccccgg gggaccaatt tttccc 356 // ID EH225066; SV 1; linear; mRNA; EST; PLN; 625 BP. XX AC EH225066; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5815.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5815 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-625 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e59bd6a13cae8621f69657602fa7e6f5. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5815.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: m column: 13 CC High quality sequence stop: 621. XX FH Key Location/Qualifiers FH FT source 1..625 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5815" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 625 BP; 174 A; 183 C; 115 G; 153 T; 0 other; ttcatcttca tctatcgttt caacttccaa gttccaactg acccagcgtc ttcctgcgta 60 catgtgagat agtaacccat gatgagatta gaactataat aatctaaaat aaccataatc 120 aactgtaaag attgcgacta tggatcaccc accaccttac tcatttgccc aggagcagta 180 ccaccccaac cataacaacg ccaacagcag ctatgaccac tttcaaccat ccagggatcc 240 aatccatccc gcttctatct acactggttt cgatgaccat cgctctcgag gatctcagca 300 ctatgtcacc agtccaacct ctagctttgt ggatacccct ccctcaacag cttcccacag 360 gatcagtcag ggtgatcaca accagactga ccgaggtctg gggggactga ttgttggtct 420 cggcgctgct gcgatgttga acaatgcgat gaatcaacaa cagcaacagc atcagcatca 480 gcatcataac ctccccaaca atcagccatt ctctaatcct cagattggta ctatcagagg 540 taggagtagt gaggatccac tggctgttct ctctcgttac gatacaatcc ttcttgtaga 600 tgacagcgcc ctcatgaacg aaggc 625 // ID EH225067; SV 1; linear; mRNA; EST; PLN; 557 BP. XX AC EH225067; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5948.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5948 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-557 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 8bc5d18ba4b893d0cfd975767a6d5cb5. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5948.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: h column: 23 CC High quality sequence stop: 491 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..557 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5948" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 557 BP; 216 A; 93 C; 74 G; 174 T; 0 other; aacttctaaa aatccctttg tattaatgta tactgataaa ttaatgtata ccgaataaat 60 gtacaaatgt gaatgactaa aaaaatagtg caataaacta attctggttc taaaaaaatt 120 aataatatac tgaataaagt gtcgaagaac aataattaaa ttaattcaag gaattaaatt 180 aaaacaataa ttatattgat caactaaatc taccatatga aaatttcatt ctttacttgt 240 aaagagccga aattatcttc accttaataa ttgtagatgt agaagaataa tcagaatctt 300 cctaaatcca cgtaatttct tcgaaaatgc tttgcccaac acaatgatat tgtcacacca 360 gaagctatgc ggcgtcttcc atttgatatt ccatttatcg cctggagatt tacaatgcta 420 atgacctatt tctgcattac aaacgttgtt gcccgaaatt ggacttctta caagtagata 480 attgtgcaca aagccggcaa tacaaatgat ccatcttatt tggagaaaag tagcccgtgt 540 tgccaaatcc aataaaa 557 // ID EH225068; SV 1; linear; mRNA; EST; PLN; 230 BP. XX AC EH225068; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5688.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5688 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-230 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3aa2bd036c22c65deeb595f082e39492. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: p column: 6 CC High quality sequence stop: 222. XX FH Key Location/Qualifiers FH FT source 1..230 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5688" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 230 BP; 38 A; 64 C; 41 G; 86 T; 1 other; cccaccactc cttctagccc ttctccagcc ggtgctgtca ctcctccccc acagaactct 60 ggtgctgcat ctcttggagt tgttggagta tttgccaccc tgctttcagt tgccgccact 120 tttttctatt agatactcca cgagattatt ctagtcagga gcttgcgagt catcatcatc 180 tctttgattg tgtttcatga attcagtttt tttcttattt ttttgaggcn 230 // ID EH225069; SV 1; linear; mRNA; EST; PLN; 566 BP. XX AC EH225069; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5642.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5642 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-566 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; dd37f930f216dbd16fc0b55a720309d1. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5642.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: c column: 20 CC High quality sequence stop: 554. XX FH Key Location/Qualifiers FH FT source 1..566 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5642" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 566 BP; 138 A; 127 C; 124 G; 177 T; 0 other; tgcgagccac acgcgttaat tgtaaaaaaa cataaaaatg ttcagcatta aaatcttcat 60 tgccctcgtt ttaatagcac agctgagcca ggcaagcgtc atttctagcc atgactcagg 120 agtgtcaacg gttaaaaatc attggaaaaa gaagaaacca tgcaccaaga ggcccgtaaa 180 agtaataccc ccaccaccac ctataggtcg accaccaata cctcacgttg gtgttgtggc 240 tggcggtata cctcttggag gcgttcctgg agtaccaccg gttgggggct tactacgttg 300 tgacggtccg ggatgtccag caggcggttt ttgtggtcca ggagcttgca gtggatgtgt 360 tagaacacct ttcggtttta gttgtggcag tgtatcttcg tttactacta cgttccccgg 420 aggtttcatg acttcatcct cttctagtag ttcttctcaa tcatcctttc ctttttaaag 480 gttataaggc tttatcaaaa agcactttgt agaaatgagg gttttttgtt ttcctaattt 540 tttttctctt ctagcttgtg accttc 566 // ID EH225070; SV 1; linear; mRNA; EST; PLN; 413 BP. XX AC EH225070; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6106.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6106 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-413 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b772a1dd68873892a452d50d805bb4c2. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6106.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: d column: 16 CC High quality sequence stop: 413 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..413 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6106" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 413 BP; 123 A; 99 C; 67 G; 124 T; 0 other; aaccattaat ttccccctta aaaaattact ttcccttcta aatcggcctt aaagtaaaca 60 aattatataa gtaatactta aaagaccgaa ttggtggttt attcgtttcc caatctattc 120 atggggattt gtcttcattc ccaaatgctg ggttgggtct actgtctagc ggatctattc 180 aatggccttt atagcatcct ttgcaagtcg ggtgacatat gcagcagcta atgcaggggc 240 caacagtccc agccccagtg ttagcaactg actgtttcct ccaaatatat tactaagttc 300 agattcatct tgaattattg ctcttccaaa agcaccagca ctgacataag cccatgttcc 360 tggaagcatc cccaaccaac ttcccaatac atatggaata aacttaacag atg 413 // ID EH225071; SV 1; linear; mRNA; EST; PLN; 640 BP. XX AC EH225071; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6105.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6105 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-640 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; f47c18600cf80cd9b663433b948b9331. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6105.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: b column: 16 CC High quality sequence stop: 541. XX FH Key Location/Qualifiers FH FT source 1..640 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6105" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 640 BP; 253 A; 101 C; 111 G; 172 T; 3 other; ataattgttt aaaagtgact caagctgtct tagctgatgt atgctcagat atatccatcc 60 acagtctcta gtctacttaa accttagaca cttagaaagg caagatgttt agctggtaaa 120 cttaaattga taaaggtaaa agtaaaaata aaaataaact gaaaccaaaa caagccagta 180 tacggtagag gatatgacga cacattccgg atacttgaag atattcatat taaaagcata 240 gatatccagt gggtctcatg cgaccttctt atcatcccaa ggctataggt atcatcatcg 300 aagtggtctc aggcataata tttcgcgacg ataaggtggc ccctgagaag aagtaccttt 360 agaatatcac gaattgttta gaatttctaa atagaatcag aaggtaataa taatatcgcg 420 agaagtttat agctggcctg actcggaaac atttcaaaag aaaaggaagc cagagaatta 480 agaaaatcaa aataaaatca gaaaaagaat taaaatttat atcttactcg acttatcata 540 acannataaa nagggataag accagtttat tttcaatttt aaaaagaaga agaatttaca 600 tcagaagtat atttaaagga ccaatgtcct tacaggatcc 640 // ID EH225072; SV 1; linear; mRNA; EST; PLN; 548 BP. XX AC EH225072; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5816.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5816 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-548 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 65488f8b74fccec3a458523db479b1c0. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5816.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: o column: 13 CC High quality sequence stop: 501. XX FH Key Location/Qualifiers FH FT source 1..548 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5816" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 548 BP; 157 A; 110 C; 141 G; 140 T; 0 other; aggacttctt taatggaaaa gagctttgca agagcattaa tcccgatgag gctgttgcat 60 atggtgcggc tgttcaggct gcaatcttaa gtggtgaggg caatgagaag gttcaggatc 120 ttctcctcct ggatgtcacc cctctgtctc ttggtttgga gactgccggt ggtgtgatga 180 ctgtcctgat ccctaggaac actacaattc caacaaagaa ggaacaagtt ttctcaacat 240 actctgacaa ccagcctggt gtgcttatcc aggtctttga gggtgaaaga gcaaggacca 300 gagataacaa tttgttgggc aaatttgagc tatctggcat tcctcctgca cccaggggtg 360 ttcctcagat tacagtgtgc tttgacattg atgccaatgg tatcttgaat gtctctgccg 420 aagataaaac cactggccag aaaaataaga tcactatcac caatgacaag ggtagattgt 480 caaaggaaga tattgagaag atggctcaag aggctgagaa gtacaagtct gaggatgaag 540 agcacaag 548 // ID EH225073; SV 1; linear; mRNA; EST; PLN; 632 BP. XX AC EH225073; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5652.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5652 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-632 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 93ce785ec288bdf5e275d50c30b8cfa9. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: g column: 22 CC High quality sequence stop: 545 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..632 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5652" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 632 BP; 154 A; 147 C; 155 G; 176 T; 0 other; tttttttttt ttttttttta agcagaagca tttctgctta gcctctccaa tttcacgtta 60 caagtttttc acaagtacta ctaatccagc tatgtagaaa atgtttaatt acaacccaaa 120 gccgaagaag aagaaaaaga cttttacaat ataactagaa tgaccaggca caagaaagac 180 tattcttctt cttttgcttc ttcttaagag gaggatgaga aggtgtcaat gatggttgtg 240 tggagtgggt cactcaagtg ggtggcccag ttgttgagcg ggcccttgcc agtggcggca 300 gcttggactg caaagcccaa gaagcccacc atggcgagac gggcgtgctt gatctccgcc 360 aattgaaggg tggctttctt ctctggatct gaggccaggc ccagtgggtc gaagtaactg 420 ccacctgggt acagcctctt ttctgggtcc agctctgcgt tcctctggaa ctctatgtag 480 ccaattacga gaacctcgat ccagatcagt gtggtgattg agaatggaag tggttgccct 540 aggtctgatg aaccttccac tagctccacc tttccggcgt cttgccatgt tacaccagtg 600 agcccactca acagtgagag ctcccgagag tg 632 // ID EH225074; SV 1; linear; mRNA; EST; PLN; 582 BP. XX AC EH225074; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6133.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6133 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-582 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ddedc337806fc998f981d16077149cb2. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: j column: 22 CC High quality sequence stop: 579. XX FH Key Location/Qualifiers FH FT source 1..582 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6133" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 582 BP; 168 A; 106 C; 125 G; 183 T; 0 other; atgtcttact gatgccaaat gggaatggag tcctaactgg gattcatatc ctttcttacc 60 aatctcaagc aatcagctgg ggagtctttg ccagaaggtt atatttacct gggaagattt 120 actctgtggt aatctatggg actattgcag gtctctttgc cccaataccc atctatctct 180 tagaaagatg gaagccgtca gtcgggttca agaaattcaa cgtgtctgtc ttcttcaatg 240 ctttacctga cctgggcggt ggtgtaacct ctggaaagat gaatgcaata gcacttggga 300 tttgggctca attctacatg cgtagatata gacggaaatg gtttacaaaa tataatgatg 360 tcctggcggc tgctcttctg ggtggaacag agataactat aatgatcctt ctgattttct 420 ttcaaggtgg agcgccttgg aaacttaaaa ttccctttta tttccttaat cctgatggac 480 ctagagacta ctgtgaagta aggccatgat aactatataa atatatacat ttaatatata 540 taaaaattag tcagtgatgt cttagactga aagaggaata at 582 // ID EH225075; SV 1; linear; mRNA; EST; PLN; 465 BP. XX AC EH225075; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5655.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5655 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-465 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 91ec215e6d27d42d160587fc9e6bed95. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5655.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: m column: 22 CC High quality sequence stop: 316. XX FH Key Location/Qualifiers FH FT source 1..465 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5655" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 465 BP; 142 A; 87 C; 115 G; 121 T; 0 other; gatttctggc caagtccatt tgggatgagg gtcaggattg cacttgctga aaagggtatc 60 aaatatgagt ccaaagaaga ggacttgcag aacaagagcc ctttgctcct caaaatgaac 120 ccggttcaca agaaaatccc ggttctcatc cacaatggca aacccatttg tgaatctctc 180 gttgctgttc agtacattga ggaggtctgg aatgacagaa atcccttgtt gccttctgac 240 ccttaccaga gagctcaggc tagattctgg gctgactttg ttgacaataa gatatttgat 300 cttggaagaa agatttggac atcaaaggga gaagaaaaag aagctggcaa aaaggagttc 360 atagaggccc ttaaattatt ggaggaacag ctgggagaca agacttattt tggaggagac 420 gatctaaggt ttgtggatat agcacttatt ccatttcgac acttg 465 // ID EH225076; SV 1; linear; mRNA; EST; PLN; 534 BP. XX AC EH225076; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5651.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5651 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-534 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 8cf534fec8395d6da7897fc1ea6fa0bc. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5651.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: e column: 22 CC High quality sequence stop: 534 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..534 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5651" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 534 BP; 150 A; 134 C; 120 G; 130 T; 0 other; cgtcccttga acaatgagca gaaggagcga tgaaactgcg gtgactgaag aagcaccacc 60 caaacagtcc ctaccgaacc tcttctcttt gtttcctaaa atcaattttc aactcccttt 120 cctcccaccc aaacctaaac ccaaacctca agaacccaac cctcaggctc agcaagaggg 180 tccgaagcct agccgtgtac agtttccgaa aacccaagtg gcggtggttg cttcttcgcc 240 tctgcaagct gaacctgatg ctgaccactc tcccgctgct aagacaacca atcctcttat 300 actctggcag atttatgcaa taggagcgat tgcgttttca tcatgggttt gggcaagatg 360 gaatgagagg aagggccgag gtggggggtc tcccaatgat gagaggggtg aaggaaggca 420 ttctgatgat ggaaatcagt gatcttcagg cgatgaatag aaatttccat tagctgtata 480 cagtgctatg ccaatactct cacattctaa gtatgaatga aaaattgttt catt 534 // ID EH225077; SV 1; linear; mRNA; EST; PLN; 631 BP. XX AC EH225077; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5645.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5645 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-631 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 62fa43638b5a5ddf12776aa527dd1bfc. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5645.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: i column: 20 CC High quality sequence stop: 546 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..631 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5645" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 631 BP; 163 A; 135 C; 126 G; 207 T; 0 other; tattattaat ggtaggaaat gcttcattat ttccttatga tgtgtaaatg ttggggttgt 60 taacttgttt tcatcctatc atttgctttg aaattttgtg gccccgttga gtgcaacgag 120 atcatcatag tattgggcga tccacccttt cataaccatg atctctctct tccattgttc 180 gaccaaccat tctggatcgc tatgatttgc atttgcaccc agaatgtcca ggcaatgaga 240 tccatttact gtatggatgg caacaagact gtctgatatg tttttcagaa ccccgccgct 300 actataaggg tctcttagtc cattagagaa aatgatgttg ctaccaaact tttgaaggac 360 caattctata ctatggcctc catagtaagt agtgacccaa tgagggcgag gtgagacacc 420 aaattgtttc ttgcagtcct tggcatagct ctttaagcta aaagggtcgg gttgaaacat 480 ggtattattt cctatgccga tgggtatcac catttcacta catgtctgcc atctccatcc 540 cagggttgtc tcagatacaa taaataggac cattaccttt gcaggtggtt gtttcccctt 600 taaggcacca aaactggata tatcttactg a 631 // ID EH225078; SV 1; linear; mRNA; EST; PLN; 664 BP. XX AC EH225078; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5645.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5645 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-664 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; cb848f37df78ea27937c4e922dc5d190. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5645.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: i column: 20 CC High quality sequence stop: 649. XX FH Key Location/Qualifiers FH FT source 1..664 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5645" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 664 BP; 190 A; 142 C; 168 G; 164 T; 0 other; gcttatggtg gatacctagg cacccagaga cgaggaaggg cgtagtaagc gacgaaatgc 60 ttcggggagt tgaaaataag cgtagatccg gggatcccga tataggtcaa cctttcgaac 120 tgctgctgaa tccacgggca ggcaagagac aacctggtga actgaaacat cttagtagcc 180 agaggaaaag aaagcaaaag cgattcccgt agtagcggcg agcgaaatgg gagcagccta 240 aaccgtgaaa acggggttgt gggagagcta ttggtgctct ggcctcatca gctccaatcc 300 tctacttcga taatattacc ccacaagacg gttactactc tgttgtttca agggatttta 360 gagaggccag tgaaacatgc taccaaacta tattgaagtc ctggtctgaa atcgacagag 420 tagcttctca gccaaagggt ctttccattc ttagccagag attcaacact tgccgtccct 480 taaatgagtc ttcagagttg aaagactacc tgattaatat gtatgcttct tcggctcaat 540 acaatcatcc gccaagatat ccagttactg tgatttgtgg cggcattgat agagcttctt 600 ttggaagtga tatcctcagt aagatatatg caggtttggt ggctttaagg ggaaacacca 660 cctg 664 // ID EH225079; SV 1; linear; mRNA; EST; PLN; 653 BP. XX AC EH225079; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5650.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5650 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-653 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 633870e0607a89d28feabd43e4a346e9. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: c column: 22 CC High quality sequence stop: 614 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..653 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5650" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 653 BP; 176 A; 182 C; 109 G; 186 T; 0 other; tttcattaac cgacctaaga gtagattact agttacaaga cactttctgg acattgctta 60 agcaggagtc caagcagaaa tggcattcag aatacgccct ttccacccca gccaaaccca 120 cttgccatcc tcagccacca caaaatcttt ttgatattcc ttcttcacca tttctttttc 180 atcattcacc aatagaggat caaatcggac ttgcttgaac ccagctctct ggttcctaac 240 ctgccactgc ttataggtct caggtctctc aatcctctca gccccttcac acgctataac 300 attgatggca tctcttccaa acaacccatt ctcaagcatc actctttccg ggtcttcacg 360 aggcacatta gcttcgaaca tatcaaacag tgatgagaag tggtagagtg cctccctaaa 420 ccgagtcagg aaaaagggtg cattgtaagt gccattgacc atcccatgga tgaacatgtt 480 tggcttgatc ctccttatca acttcaacac ggcatctctt ggagacttca catccacagt 540 ttcatcaggc aagttcttca gccggtagaa gcagctaaca acagttactt cattcctgtc 600 tatcttgaga tctgcaagtg catagttacc atttctgtgc atgacattgt atc 653 // ID EH225080; SV 1; linear; mRNA; EST; PLN; 575 BP. XX AC EH225080; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5823.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5823 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-575 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 7b6a39e113a0ae4b47c62e5b8c2dde0d. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5823.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: m column: 15 CC High quality sequence stop: 527 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..575 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5823" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 575 BP; 186 A; 131 C; 85 G; 173 T; 0 other; actagtgact caaatatatg tataatatag gaaatattct gttacaaaac caacaaacac 60 atgaaagata catcagaaaa tttttaaaac ctacgcactc ctacaaaaag catgaagttt 120 ggtacatcca agtgccttta atttactaca caaatactat ttagttgaaa ggccaagaat 180 gatcaatacg tcactatggc tattaaaatc caacatgaac ctactcaatg tcgaacttct 240 ttcttatgtc cataatgaac tcatagacct tgtgttgatc aggaagagac ttggcaacac 300 tgtctttctg caggcacctc ttggcccaag caacaaactt ggggcactca ctctctatgt 360 tgaggctgcc aaaagtcttg aaccaagtgt cgaatggaat aagtgctata tccacaaaac 420 ctagatcgtc tcctccaaaa taagtcttgt ctcccagctg ttcctccaat aatttaaggg 480 cctctatgaa ctcctttttg gcagcttctt tttcttctcc ctttgatgtc aaatcttttc 540 ttccagatca aatatcttat tgtcaacaaa gtcag 575 // ID EH225081; SV 1; linear; mRNA; EST; PLN; 634 BP. XX AC EH225081; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5664.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5664 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-634 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 28ff59dacd3c200b6c4cdad7fe7e910d. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: o column: 24 CC High quality sequence stop: 634 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..634 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5664" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 634 BP; 227 A; 128 C; 115 G; 164 T; 0 other; ggtagtagta atgataaaaa tatttacact caataacaaa gtttctgaaa ccaacccctt 60 tcgaaataca aaccaaaagg ttaaaagcta ttccacaaag ccgaaattta ttataactga 120 taagaatata cagtgagtga gaaattgttc aaaacggaga aaaagaatct ttaagattta 180 gattttactc ttttatttaa tttttaactc atacttgaat ttgttaaaac tagccatcaa 240 gacactaatg tccatttttt cttttagaac ttttctcggc ttgctctgct gcatcaacag 300 catctgcatc gtaccccttc gtttcaggaa gcaaaaacgt aaaagcaaga ccacaaatgc 360 tcgtaccaac caagatccaa agcacagtag gtgtaccatg atccttggca agttcagaaa 420 accctagtgc agagagaaca gctccgagct ttcctccggc agccgaaaat cctgatgcaa 480 agcttctcac gcgagttgga tatagctcag aggcgtagat gaaagtagta gcatttgcac 540 caaagttaaa gaaaaattgc agcaaggcaa aacagacgat aaacccggca ggcttatgtg 600 acaggcttgt atagtcagcg gcaataatag taag 634 // ID EH225082; SV 1; linear; mRNA; EST; PLN; 538 BP. XX AC EH225082; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5826.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5826 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-538 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 8c14e14a181494c617efbe82289c4ee2. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5826.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: c column: 17 CC High quality sequence stop: 470 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..538 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5826" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 538 BP; 195 A; 94 C; 85 G; 164 T; 0 other; aaggaggaaa ttttgtcttc cactcactcc aacgggaagc agctaagttg ctgcaagaca 60 tttttttttt acataacgaa aatgaaattt aaacaaaagt tacttaaatc ctccattact 120 aagttgacct tagtgaattt aattactgtt tgacataaac ataggatatc ttaattcgta 180 atagcaactt aacgagacat cttaacgatg tgtgcaatca agtcaacaat gtaattgctg 240 caggaccaca cacaaagtag gcattagaaa aaataaacat ataaacaaat atattttaaa 300 catgcactag tttatttata gtaaagcatc tacctttctc gtgagaatta gattttggtt 360 ggatgaacaa tgaaatggaa gtcaagcgca ctctcattaa ccttgtatct taacaaattc 420 atgctagctg cactattaca ccataaatca ttatgcaaga aagggtagaa ggtgaaggtg 480 gattacctgt aaccccactc gttattatac catgtgatca gcttcagaaa tttttcat 538 // ID EH225083; SV 1; linear; mRNA; EST; PLN; 619 BP. XX AC EH225083; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6121.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6121 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-619 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 75ba6bd54118285662ad46e53b477f86. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6121.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: b column: 20 CC High quality sequence stop: 575 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..619 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6121" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 619 BP; 202 A; 146 C; 114 G; 157 T; 0 other; aagataaatg cataagaact tatattgtat cctcgttgcc acaattggat acagaaaaag 60 atacgttgta ataccgcatc tattataaaa gtagtctaac attaatttaa atgacaacta 120 ttaatatgaa aaggggatcg aaaaaaagga atgaaccatt cttcaaacat tattattctt 180 catgtttaac acgagcatcg tagacacatt gtcttagtta cagaactgca tactattcaa 240 cggctcatgc cattttcgac tgatggagct gttgtagcag tcatggctac tccccgactt 300 cgttgctcga catttccatg ccacccaata atcatgtcaa atccacggtg ttgaagctca 360 cgatattctt gacagagggc gcagggctcg cagaagcaat gaatcaagca atccgaacaa 420 ccatttccct ttagaccata ctgtcgtctc atcttggggc ggtagaagca agagtatagg 480 cagccacagc caatgacaca gcaaatcagc gtatacagag ccccactagc accacatgat 540 gtggatccct tgtcaacaat tttctgcact cggccaaggt aacacatgga caccaacacg 600 tcatgcaaca gtttccaca 619 // ID EH225084; SV 1; linear; mRNA; EST; PLN; 546 BP. XX AC EH225084; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5974.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5974 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-546 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 8e24796c70c68e2958adac6be07a880c. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5974.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: k column: 6 CC High quality sequence stop: 464 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..546 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5974" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 546 BP; 178 A; 142 C; 86 G; 140 T; 0 other; ctcccctcaa caaaacaaat tgtaaaattc tattaagctc tcctttccaa ttttacatca 60 tgaccctgcc tcaacaatca gccaaatata caaagcacct aagatgaccc acaccggtaa 120 ccataataag tatcacacaa ggagcagtta gttcattgaa cttgtatatc aggaaggaac 180 caataccatc ttcgtttgta agtccctcaa aatctgagct gaaaaaatca aaagctcaac 240 catttcgagc cctgctgcag aaacagtatc catcttctca tcacagcatt cctgatgccc 300 tgtgtcactg cccacattgt atccaatata actaatatct gcctcagccg accaactggt 360 gggtaaggag ctgccaagag cagtccttaa aagttctgcc tcatgaataa cgtcattaag 420 gatttctgaa acaccggaaa ttgtaccttc cgaaatcaca gactccgtgg cctttaaaag 480 agagagacga agcctatgaa ttgatgaagc taaacgctcc atgctttcca tgctttcctt 540 taaagt 546 // ID EH225085; SV 1; linear; mRNA; EST; PLN; 450 BP. XX AC EH225085; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5977.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5977 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-450 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ed4834c381c6c4652bd59f526766a4a0. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: a column: 8 CC High quality sequence stop: 268 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..450 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5977" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 450 BP; 152 A; 106 C; 79 G; 113 T; 0 other; aaatcaataa taaattaagg aaagtaacac tgttttataa caacgaacaa actatttgat 60 tattaaggga attctaatat tgataggggc ggaaatgtta gtgggtgcca ttgattatga 120 agggaattct aatattcaat ggcaaaacta acatcttcac ttggttacat caaaacaatg 180 gctcaactct caagccacat ctatatgtgg ctcaaccact gctcttggct attgcaaaac 240 actttcatgt cttccctgaa agcagggaac gcagcctctc tcaaagtcac caaaaccttg 300 aacccctctt tcttctctga agtgggtgtt gaataaggca aaaaaaagca aggctcaaca 360 ctcccaagta agttccttcc aaggggcaaa acaataactg ggccacccca cccaaagtcc 420 acagttgaat ggcccaagtg cctcccatcc 450 // ID EH225086; SV 1; linear; mRNA; EST; PLN; 639 BP. XX AC EH225086; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5827.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5827 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-639 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 8265e080981b144a26af741f52c27515. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5827.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: e column: 17 CC High quality sequence stop: 629. XX FH Key Location/Qualifiers FH FT source 1..639 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5827" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 639 BP; 183 A; 135 C; 167 G; 154 T; 0 other; ctaaaaagcc agagaaaaca aaacccccaa aatatctctc gctctgaaac cggcatggat 60 ttgcagcaga gcgagttcga tcgcctcttg ttcttcgagc acgctcgcaa ggccgcagaa 120 gctgaatacg agaagaaccc tctcgatgcc gataacttaa caagatgggg tggagctcta 180 ttagagttgt ctcagtttca gagttttcct gaatcgaaga aaatgaccca agaggctgtt 240 tcgaagctag aggaggcgct tgcagtgaat cccaagaagc atgacactct ttggtgcctg 300 ggaaacgctc acacttcgca agcgttttta attccggacc aggaggaggc aaaggtttat 360 tttgataagg cagctgtgta ctttcagcaa gctgttgatg aggatccttc aaatgaactt 420 taccgcaaat cattggaagt ggctgctaag gctcctgagc tgcatgtaga aatccataag 480 cagggatttg gtcagcagca gcaggcggca gcaacagctg gatcttctac ttctgcttcg 540 ggtacaaaga ctcagaagaa aaagaagagc agtgatctga agtatgacat atttggatgg 600 ataattctgg cagtcggcat tgttgcatgg gtgggattt 639 // ID EH225087; SV 1; linear; mRNA; EST; PLN; 524 BP. XX AC EH225087; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5657.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5657 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-524 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 4fa105049fda6ad43fd6e9a19cc37e40. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5657.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: a column: 24 CC High quality sequence stop: 524 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..524 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5657" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 524 BP; 190 A; 124 C; 90 G; 120 T; 0 other; aaattaaatg cgacgtaaaa catccctcag cagcaaagga aatggactaa aaccctatag 60 ctgcttgtga aattcgaaat atctcagact cttcttacca tcaaaacaaa aaaacagtac 120 tcatcaaaaa caaacagcaa tgaaaattga ccaccgcaga tcaagaacct agttttgaaa 180 gttacggcta catgaaatcg gcagaaactt aaaaatataa cttaaacaaa acacctaaga 240 ctctgcaagg ataaaactac tataaaggag taaccacgat gatgatcaca tgtgctagca 300 aaattgccca aatgcaatca cttcttcttg gcagcggcct tggtgacctt ggctccggtg 360 gggtattctt ctcaacactc ttgatgactc cgacagccac agtttgacgc atgtccctca 420 cagcaaaacg accaagggga ggatactcag agaaagtttc aaccaccatg ggcttggttg 480 gaatcatctt aaccatacct gcatcaccat tcttcaaaaa tttg 524 // ID EH225088; SV 1; linear; mRNA; EST; PLN; 554 BP. XX AC EH225088; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5828.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5828 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-554 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 54fc03da39741fac5b6fce9e7f727da8. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5828.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: g column: 17 CC High quality sequence stop: 497 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..554 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5828" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 554 BP; 167 A; 106 C; 103 G; 178 T; 0 other; gagaaagaga agccaattat tgtctcaatc ttgtatcaac ataactgaat ctcagcctcg 60 ctttttcttc tcggataagt acatacatac atagtgtttt tttcattaat tatcatgcag 120 aacagcctaa taacaattga caaaataaca tgaaatagag tggaattgtg gaaaacatga 180 aagaagaaag aaaatgatga ttttgatgat gagagaactc aatttggctg cgttgctcga 240 tcgatatatg tatatatatt atggcaggtt caattcctaa gcttttgcag ctactggagt 300 ctcagccttg ctggctatta gctgctcata tagatctctt atcttcaaac caactatggt 360 ttggaagaga cctgttccat tgttgctgcc gggaaaatca gcatgcttca acatctgttg 420 agtaacctct ccatagaact ttgtagagag gttgcttagg tggctctcaa cgtatattag 480 ttcatctagt ctgtcaaatt gtccatccat ttctacaact gacacctcat ttccgtagta 540 agtctcaggt ccat 554 // ID EH225089; SV 1; linear; mRNA; EST; PLN; 610 BP. XX AC EH225089; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5982.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5982 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-610 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; fa850bc870d4b2c54def62cdcb9c1cc1. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5982.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: k column: 8 CC High quality sequence stop: 607. XX FH Key Location/Qualifiers FH FT source 1..610 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5982" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 610 BP; 213 A; 97 C; 94 G; 206 T; 0 other; ttttctgaga taggttgagt ttttaatcga atcgtttttt tgaaaagtca atcgttctta 60 actaaagata aaaaaaaacc gcatctcagc agcgcaaata aactatcaat cagatttatt 120 aaaagaaact taagtataga tcataaatgg ttctagatat taaaaaactt gatggtttct 180 ctgttgagag cctttacaaa ttccagactc gaacagtcat tctgatattt ttgatagctg 240 ttacactatt tgaatcagct cgtgctgcag tgatgacagc gaaagatacc gaatgctttc 300 gcttcaagta ttgcgcctgt cgttgctcta ttgtttgagt gaagatttac agctaacgga 360 gttgttaaaa aattgccatg tcttttattt ggaagctatc aacaaactag aaagatacaa 420 gaagaaacaa aagtatttta aaattcactt caaatattta tcctgaagac ctcgtataat 480 atacatttgt aaatcctttt atacattggg acctaaaaaa ttatctaatc ttcaattcta 540 aaatttctac tagtaactta gctataagaa agtcatcaat gatgtttttt ctgatatcct 600 gcaaaagagc 610 // ID EH225090; SV 1; linear; mRNA; EST; PLN; 461 BP. XX AC EH225090; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5981.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5981 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-461 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; ab97ee97d51aae9f9c59bc683ae1878d. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: i column: 8 CC High quality sequence stop: 403. XX FH Key Location/Qualifiers FH FT source 1..461 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5981" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 461 BP; 116 A; 116 C; 118 G; 111 T; 0 other; aaatgaatac cctcctagag actcttgcac gacgggatgt ccgcaagctc ttcccctgct 60 ctggagggag gggaagcaag gcttctactc actcactcgt tgtagaagct tccaggctac 120 caaagctggt ttacctagga atagacgtta gatctaagaa gtccaactgt cgccacacgc 180 cctgagcgtc tggtcaagta acgttctctg aacgttaccc gtcgatctca aaagagagag 240 actgtccgct cgtatatcga gcggactatt actacttgtg aggtgtcaag agaaatcttc 300 ggcatccagg tcctcccgaa ggaggggtgg ggcttacgct tcatctttct caagggtgac 360 ctagagtagc agtttggggg tgtcaaaccc cgttttttga atcaaagggc ttgtgcataa 420 tccaacctat gaaggatgtg tgctacaggg accaccaacc a 461 // ID EH225091; SV 1; linear; mRNA; EST; PLN; 616 BP. XX AC EH225091; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5829.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5829 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-616 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; c93ca861b0425f6598591f7a35ea27f4. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5829.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: i column: 17 CC High quality sequence stop: 562. XX FH Key Location/Qualifiers FH FT source 1..616 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5829" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 616 BP; 183 A; 109 C; 115 G; 209 T; 0 other; ctcaagtaaa ggagttgttg gatgagggtt tggttcgtaa gagcttaaat ccttgtgctt 60 tgttggtgcc aaaataggta ttattaggca ccaaatccct aaaataagtg atatgatgaa 120 tgtgttgagt gttgaaatcc tctttcgtaa aattagtcat gcacccaaca ttttcatgag 180 tcatgtacat agggactcag taggtaggtt tgttcttatt tttggttttc atacaaactt 240 aggtgctcat atgggacacc ttaggtttgt catatttttt ttgtaggaat aatcaacatg 300 aaaatataga aaaaggacgc tttattgcat tactttcctt aattttttaa atagtgatca 360 aggggttccc acggacccta agagaatata ggtcattcct gaatggccta ctccactaag 420 tataagggaa atctggggct accatgactt aacaaacttt tacaaaaggt ttttcccata 480 tttttctata cttgtagcac cactcattga gttggtgagg aaccatgttc cctcatggga 540 agatctccat gaaaaggttt ttcaaacctt accctactct aacataccca acaccactaa 600 tacatatgtt tttatt 616 // ID EH225092; SV 1; linear; mRNA; EST; PLN; 634 BP. XX AC EH225092; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5988.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5988 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-634 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 49d9a2b173317c9dc4288b0838205c66. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5988.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: g column: 10 CC High quality sequence stop: 599. XX FH Key Location/Qualifiers FH FT source 1..634 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5988" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 634 BP; 144 A; 159 C; 137 G; 194 T; 0 other; atttatgcgt gtcttccccc aacgaaacca acgaggaagc aagggggatc ttgaattctg 60 cgcttaacgc ttcgaatctt tgaattcatt caaagatctc cctctacgca tatcagggca 120 ctatggctgc cacggtgaaa caaatgtctt tgatcgtgtc actgttgggt gtggtgtcct 180 tcattttggg agttgttgct gaaaacaaga agcctgtggc tggaacaccc gtgcctggtt 240 cggatggtat ctctgtcacc tgcaagtacc cagccgatcc aactgttgca ttggggtatc 300 tttctacagc atttcttgtt gcatccactg tgattgggta tatgtctttg ttttaccctt 360 acaaaggaaa gtctattcca caagggattc tgtttaagca caccactttc acagtgttct 420 tcaacatttc tttgttcacc gctggattag ctgcagctat gctgttatgg ccaacaatca 480 cagaacacat tcacctgaca cgcaatgtgc accagaatct cagctatgaa tgccctacag 540 ccaaaactgg tctcctcgga ggcggcgcct ttctatccct tgattcatct ctcctttggt 600 tgattgccct tatgttagct ggcaatgctc gtga 634 // ID EH225093; SV 1; linear; mRNA; EST; PLN; 388 BP. XX AC EH225093; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5673.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5673 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-388 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 9c035eb803bc377451be202cb4cd3de0. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: b column: 4 CC High quality sequence stop: 317. XX FH Key Location/Qualifiers FH FT source 1..388 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5673" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 388 BP; 113 A; 73 C; 83 G; 119 T; 0 other; taatgcttta aaaactggaa gccaacgtgg tggtgttgca gctatattcg atgaagtgcc 60 ttaccttaaa gtcttccttc aagaatacgg atccaattac atcatggccg gctcgagata 120 tagaaatgat ggctttggtt ttgcattccc tttaaactcc aatctcacca ctcacttttc 180 gagagccatc ttgaaggtga ctgagagtga gttaatgaat gaaattgaga gaaaatattt 240 tgggaagaag gatattgaag aagactcgtc tgcggaaatt tcttccgctg ctccaagcct 300 caaactttca tagctttgcc agggctattc ttgattacag ggattagtaa ctttattagc 360 actaatggtc tcagaaactg ttaattgg 388 // ID EH225094; SV 1; linear; mRNA; EST; PLN; 680 BP. XX AC EH225094; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5663.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5663 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-680 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 47756510a309ad9a54e9f86d537399e7. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: m column: 24 CC High quality sequence stop: 680 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..680 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5663" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 680 BP; 195 A; 144 C; 106 G; 235 T; 0 other; agttataata attgggttat gtctttcagg aagcaagagc aaaaaacaat aggactcatt 60 cttttaaaaa tgtttaaagt acgcaaaaat cgatcagatc aaataaataa aacgaaacaa 120 atctttgatg aaaaaacaaa gaaattttat gttttaaatt taattttgtt taacttcagt 180 tttttctgcc gtcaaaataa ataaataaaa ttatattatc tatttatgga aaccttcctg 240 ttctaaaact tggtcttctc ccctcaatct agcttctcca gcttcgactt cgtgtggctt 300 tccaaaagtc tttcctgcaa actttttggc atatccttca actttagcct taaaggggac 360 tttaggatgc tcgtctgccc caacgtttcc aatgttatca ccagtggttc cagggtgagc 420 tctcccacca tggccttgga aatggccatc gccaccacca agttgattct cgtttctaaa 480 tttagaacct tcgtccgaca ggtgctgttt tgcttggtct gtatctacaa cattcccaaa 540 cccctgtctg ttcgggtcaa actcattaga tctcacctga tcattgagac cagagccagt 600 agtagattga ttatttctat tgatcgaact catggctcta ctgcttcttg tttttttaac 660 ttctaagaat tgcctttttt 680 // ID EH225095; SV 1; linear; mRNA; EST; PLN; 686 BP. XX AC EH225095; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5682.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5682 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-686 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e3c1f21dd770b2664292f5655dd53464. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5682.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: d column: 6 CC High quality sequence stop: 629. XX FH Key Location/Qualifiers FH FT source 1..686 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5682" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 686 BP; 242 A; 105 C; 144 G; 195 T; 0 other; aaaaaaggca attcttagaa gttaaaaaaa caagaagcag tagagccatg agttcgatca 60 atagaaataa tcaatctact actggctctg gtctcaatga tcaggtgaga tctaatgagt 120 ttgacccgaa cagacagggg tttgggaatg ttgtagatac agaccaagca aaacagcacc 180 tgtcggacga aggttctaaa tttagaaacg agaatcaact tggtggtggc gatggccatt 240 tccaaggcca tggtgggaga gctcaccctg gaaccactgg tgataacatt ggaaacgttg 300 gggcagacga gcatcctaaa gtccccttta aggctaaagt tgaaggatat gccaaaaagt 360 ttgcaggaaa gacttttgga aagccacacg aagtcgaagc tggagaagct agattgaggg 420 gagaagacca agttttagaa caggaaggtt tccataaata gataatataa ttttatttat 480 ttattttgac ggcagaaaaa actgaagtta aacaaaatta aatttaaaac ataaaatttc 540 tttgtttttt catcaaagat ttgtttcgtt ttatttattt gatctgatcg atttttgcgt 600 actttaaaca tttttaaaag aatgagtcct attgtttttt gctcttgctt cctgaaagaa 660 ataacccaat tattataact aaaaaa 686 // ID EH225096; SV 1; linear; mRNA; EST; PLN; 504 BP. XX AC EH225096; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5669.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5669 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-504 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 80d971b93d0f131dd0cd3876a9a3a7aa. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5669.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: j column: 2 CC High quality sequence stop: 504 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..504 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5669" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 504 BP; 170 A; 86 C; 93 G; 155 T; 0 other; ctttggagta gtcctactag agattcttag tggaaaaaag aacaccgggt tctatcagtc 60 caaacaaatt tctagccttt tgggtcatgc atggaagtta tggacagaga agaagctact 120 ggatttaatg gatcaatccc ttggtgaaac atgcaacgaa aatcaattca ttaaatgtgc 180 agttattggg ctattatgta tacaagatga accaggtgat cgacccacta tgtcaaatgt 240 tttgtacatg cttgacatag agacagcaac catgccaatt cccacgcaac caaccttttt 300 tgtgaacaag catttttcta gttcagcttc ctcttctagc aaaccagaaa taagtctgca 360 atttgagagt agttatcaag aaggtcgata gtccatgatg gatcctatag ttagaataca 420 aataaaataa gaaaaattta gagacatgta ttaattttaa ggattgttgt tatcttataa 480 ttcatttcct ttttaacaaa gagt 504 // ID EH225097; SV 1; linear; mRNA; EST; PLN; 486 BP. XX AC EH225097; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6135.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6135 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-486 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 79d03f3f50a5a0adc3ef9074e0feb0a3. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6135.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: n column: 22 CC High quality sequence stop: 233 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..486 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6135" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 486 BP; 165 A; 109 C; 86 G; 126 T; 0 other; gaagaaaaca cttcaacagc catcatacat agttcttcca ttcaaagctg taacatctaa 60 aatattctga tggggcatgg aagcaaaatc agatcaagag ggatcaatta aaaacttatc 120 ctgcatatgg atgcttctct attctctaaa aaccaaaccc tggattctat ctcagattgc 180 acttttaaca taaatcaaga agatgttccc ttgaagataa gaaaattaag agtcgcccga 240 gcaaaataga tgaaagtccc tgatgaatgg gtgaaatagc acccaaaatt ggatcctacc 300 acacattgcc acccattccc gtacacacta tcaaattcct tcttgatatg ggcagcaata 360 gagatgcaat cataaacatc gtagagatca agggcctgag aagcagaggc catggcatgg 420 atctgcatct tggttgacat gtctgtttct ttcaccatag cctttccttc caacatcctt 480 agagag 486 // ID EH225098; SV 1; linear; mRNA; EST; PLN; 567 BP. XX AC EH225098; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5837.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5837 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-567 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 5114d73ce69add6ffce9e4b3a7dd2cb8. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5837.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: i column: 19 CC High quality sequence stop: 566 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..567 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5837" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 567 BP; 170 A; 123 C; 104 G; 170 T; 0 other; aaagtaatct aaggtccatt tattgatttt cctttgctgg gttacacatt tatttatgtg 60 aacaattcaa agttcttcgg aggactttga attatttgga acattacaac aaataaaaag 120 tcaccttacc cttcccttcc cgaacaccca aacaactccc gaattattcg caaacagatt 180 tattaacaac aatgaatcta actacccttt gcaaatcccc ccgcactaaa attgcaactt 240 gtgttgttgt ttacctccaa aacatgcact gtttgcgact ttacatgatc aaccaaagcc 300 ttaacttaaa gcacagaaca atcggtacga attttcccat ttcttgcagt cttaactcca 360 acgcgaccca atttggtcat agcagccaca aagttggagt tgaaaacgtt ggtactggaa 420 gcaaaggaat taacggtgtt cctagacctt gggtccgtga agaggatttg gtccgaggtg 480 aagaggccct ttccttgttg aaggttttgg taataaacat tgtcgaattt acgaggagtt 540 gttgggtcca tgttgatggc aatccta 567 // ID EH225099; SV 1; linear; mRNA; EST; PLN; 400 BP. XX AC EH225099; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5838.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5838 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-400 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 6f7cc313ff3f5413f050786509703e74. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: k column: 19 CC High quality sequence stop: 363 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..400 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5838" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 400 BP; 91 A; 99 C; 79 G; 131 T; 0 other; ggcaaagcta aaatatttta tatttggatt tggatttgtt ggcacccagc tgaaatgcca 60 tgccgtgctt tacaacatcc gtgcatttcc tacacatcct tagcaaacat aaagtttagc 120 cctctcttct actcattcac ttctgtctct attcagaggc tgagtccatg tttcccattg 180 ctttcaaacc tcttgggctc tttttggata tcttcttctc cctaacctcg tatcgttcca 240 agggaagatc gcacaaccat gcgccaggac taacaagatt gagggttcgt tgaatgaatt 300 tagctctctt cttgggtctc tgaggcagct ttgagccctt aatggcgaga aaatcttctt 360 ccttctcctt gttgggcaag gcaatcacga acttgggtgg 400 // ID EH225100; SV 1; linear; mRNA; EST; PLN; 200 BP. XX AC EH225100; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5672.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5672 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-200 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 7bfff4bef2db6af3a7f1828dc23f639b. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5672.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: p column: 2 CC High quality sequence stop: 200 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..200 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5672" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 200 BP; 79 A; 39 C; 33 G; 49 T; 0 other; tcctaaataa acaaaacatc tctcaggtac ccagtcagga atggccacat gtaaagagag 60 gagccacaac taatgacaaa cgagtgaaaa cataaagatc aaatacataa agactgcagt 120 ggatgggtac aattgtacct ttggagttta aacttgtcac aaactaatac actagatttc 180 cttcatctct aaaatgattt 200 // ID EH225101; SV 1; linear; mRNA; EST; PLN; 429 BP. XX AC EH225101; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5992.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5992 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-429 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 9efee00f709b0c43c0d85d41e7e8f8d8. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5992.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: o column: 10 CC High quality sequence stop: 348 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..429 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5992" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 429 BP; 150 A; 106 C; 77 G; 96 T; 0 other; agggccgaaa gatcactatc attatcgctg aacaatgtta tttaacaaaa gtatcaaccc 60 caaataaaaa caacaaccca acttcccaat catcgtcacg tccagcaaaa tacattcaaa 120 agctatctaa aatctgggga acccacattg gcattaaaaa ccatgaccat acaatttttt 180 agagaaatat tatgggcacc atgcctcttc aacaatcccc taaacgttgc ttaggcatca 240 gcaaacccaa gctcggaaag cttttggtga gcctcagcgt aatcagcaaa gaaggcatct 300 tcgtccgctg catatttatc aacgagaggg cggaatacag ggtcagacaa aagagccttg 360 tcagaaggta gctgaaggag accttccttc tcaccactca acaactccgt gaagtatgag 420 ttgtcgaaa 429 // ID EH225102; SV 1; linear; mRNA; EST; PLN; 645 BP. XX AC EH225102; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6137.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6137 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-645 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b655df49d581eefe94a509562e7fe394. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6137.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: b column: 24 CC High quality sequence stop: 563. XX FH Key Location/Qualifiers FH FT source 1..645 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6137" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 645 BP; 165 A; 147 C; 174 G; 156 T; 3 other; aaccccttcg gcgttttgtc cttacgttgt tattaagaag cgtaacgcat tcaccacgat 60 ggtgaccaaa gcgatggagg tggtgaagga tctggacgtg aagcggtaca tgggtcggtg 120 gtacgagatt gcgtgcttcc cgtcgaggtt tcaacccagt gacggcacca acaccagagc 180 cacctacact ctccgagatg acggcaccat caacgttctt aacgagactt ggagtggcgg 240 caaaagaggt ttcattgagg gcactgctta taaggctgat cccaacagcg acgaggccaa 300 gttgaaggtc aagttctggg tgcctccctt tttacccatc attcccgtta ctggggatta 360 ctggcttttg tacattgacc aggattatca ctatgctgtc attggccaac cttccaggaa 420 ttatctttgg atattgtcca ggaagaacca tatggatgag gaaacctaca accagcttgt 480 tgagagagct aaggatgagg ggtatgatgt gagcaaactc cacaagactc cacacagtga 540 ttctccaccg gaggaagaag gtcctcagga caccaaaggc gtttggtggn atcagtccct 600 tctggggaaa ntagtgtggc tgaangtgaa ggagtgtttc atatc 645 // ID EH225103; SV 1; linear; mRNA; EST; PLN; 483 BP. XX AC EH225103; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5679.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5679 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-483 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 7f00cac2714d01d5688f302ccd9cac40. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: n column: 4 CC High quality sequence stop: 397 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..483 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5679" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 483 BP; 151 A; 109 C; 89 G; 134 T; 0 other; ttttaccaag aaaacctttc tttctagctt caatttattc aaactcattt gctagaagtt 60 gaaaaaaaat aaaatacagc acatctaagt ccccttcgaa ttcactgcta agaaggaatg 120 ataaataact aaaagaaaac aactgaaagc caaagactcc aaaagtttta gtaggtatag 180 cccttgacaa acattccctt cttggcctct tcactctcac cctcagcaga gtatcttcca 240 agttgagcca aggagtttgc tttagcacgc accaaaaggg acttttgagc agcttccaca 300 ttctcagggt gtccttgcca tgtcttaagc acagtgttct gaagagcccg tgcatatgag 360 aaagaaacat gccatgggtt gggactttgg ttcatggcat ttagatttag tgttgcttcc 420 acttcggatt gtccaccaga caaaaaactg attccaggga cagcaggagg aactcttctt 480 cta 483 // ID EH225104; SV 1; linear; mRNA; EST; PLN; 227 BP. XX AC EH225104; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5672.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5672 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-227 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; abf99a03036b6c7a973c3b67aba70827. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5672.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: p column: 2 CC High quality sequence stop: 227 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..227 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5672" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 227 BP; 60 A; 35 C; 42 G; 90 T; 0 other; aaatcatttt agagatgaag gaaatctagt gtattagttt gtgacaagtt taaactccaa 60 aggtacaatt gtacccatcc actgcagtct ttatgtattt gatctttatg tttcactcgt 120 ttgtcattag ttgtggctac tctctttaca tgtggccatt cctgactggg tacctgagag 180 atgttttgtt tatttaggaa ttaatataaa atgatatctt gtctgtc 227 // ID EH225105; SV 1; linear; mRNA; EST; PLN; 575 BP. XX AC EH225105; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5844.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5844 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-575 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 17ab2678a61ff5d12836d597d6edf5a6. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0061 row: g column: 21 CC High quality sequence stop: 567. XX FH Key Location/Qualifiers FH FT source 1..575 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5844" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 575 BP; 143 A; 121 C; 141 G; 169 T; 1 other; cagggcctca atgctgttca aatccaccaa gcttgaaccc cggtggagga gttgggcatg 60 tggacaaagt gggtggtgtt gattcctatt tcactggatc tcctcactcc aaactcgctg 120 ttcttatgct ctccgatgtt tttggatatg aagcgccaaa tttaaggaag cttgctgaca 180 aagttggggc tgctgggtat tatgtagttg ttcctgacct cttggatggc gaacccttta 240 atcctcagaa ttcggacagg ccctttcctg cttggataaa agatcatgga ccagtggaga 300 aaggtgctga agctacaaaa cccataattg aagccttaaa aagtaaaggt gtttcagcta 360 ttgcagctgt tggtttttgt tggggtgcaa aggttgtggt tgaacttgcg aaatccagac 420 tgatccaaac tgcagtgcta ttgcatccat catttgtctc cctggatgac atcaagggtg 480 ttgatattcc aattgctata cttggagccg aggtagacca agtttctcct ccagagcttg 540 tgaaacaatt tgaacaagtc ctagctgctn aatct 575 // ID EH225106; SV 1; linear; mRNA; EST; PLN; 313 BP. XX AC EH225106; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5676.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5676 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-313 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 5a9eccd636059b146ff281c0f444b7b6. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: h column: 4 CC High quality sequence stop: 233 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..313 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5676" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 313 BP; 101 A; 58 C; 50 G; 103 T; 1 other; tttttttttt ttatttttgt gaacaaggag atgctagata ctttcttagt caacttgaca 60 agagattata aaacaatcag cataaacact aagcacaata aacaatcaga atgttttcac 120 tgtctgagga aatttacagc agnccaaatg gggaagatca tgataatgcc ttcaacttgt 180 ctgaacattc gtcatgatct ggtggtgctt tgaagttctt cactgattta cccaagacat 240 ttaaaacagg aacacgctgt aattctttag cttcctctaa tgaaagctga attgatctct 300 tctctaactc aac 313 // ID EH225107; SV 1; linear; mRNA; EST; PLN; 511 BP. XX AC EH225107; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5843.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5843 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-511 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; f2a26b29d9aba4df02426557bbc60942. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5843.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: e column: 21 CC High quality sequence stop: 496 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..511 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5843" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 511 BP; 161 A; 118 C; 76 G; 156 T; 0 other; aaagttcaaa caatacttta tagctgagat agttagcaaa catcccacat ttacaacact 60 aacaagcagg ctgacttgta tgattagtag tattgcatga aaatctatga tgcctgcacc 120 ctccaagtga gatgaataga gaggcagcga caaatatacc aaattaaaca aacaaacaaa 180 ctgaattaat taaacaaaag tgcgagattt tatcattcaa atctcaaaac ttcatatact 240 tgcaatattc ctttactttt ctgttctgtt ttttctcttc atctatttga caggttcttc 300 gtgtccttag ccatgaaaac agtatctata tgggcttgca tctctgtttg atgttttctt 360 ccaatcttgg tgcaagtcca ttagcctcag atgtcctcat gattcttagc ctcctcaccg 420 agctaaggaa catcccccag ggaacatccc caacaagcat ccaatctcct tccttgtctt 480 cataagtgag cacaaacttg gaagatccat c 511 // ID EH225108; SV 1; linear; mRNA; EST; PLN; 684 BP. XX AC EH225108; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5843.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5843 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-684 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 6fd54f88be747a63efc66f311e6d913c. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5843.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: e column: 21 CC High quality sequence stop: 684 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5843" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 684 BP; 214 A; 102 C; 159 G; 208 T; 1 other; acgggatacc aattggaagg aaggttgatt tgagtgccca tagttcctat gaaaccttag 60 cacaaacatt ggaggatatg tttaatgaat caactacagt caccacttgc aaaggatcaa 120 atggtgagga ttatggcatt attatcggag gagaaagaca ttcaaaattg ttggatggat 180 cttccaagtt tgtgctcact tatgaagaca aggaaggaga ttggatgctt gttggggatg 240 ttccctgggg gatgttcctt agctcggtga ggaggctaag aatcatgagg acatctgagg 300 ctaatggact tgcaccaaga ttggaagaaa acatcaaaca gagatgcaag cccatataga 360 tactgttttc atggctaagg acacgaagaa cctgtcaaat agatgaagag aaaaaacaga 420 acagaaaagt aaaggaatat tgcaagtata tgaagttttg agatttgaat gataaaatct 480 cgcacttttg tttaattaat tcagtttgtt tgtttgttta atttggtata tttgtcgctg 540 cctctctatt catctcactt ggagggtgca ggcatcatag attttcatgn catactacta 600 atcatacaag tcagcctgct tgttagtgtt tgtaatgtgg gatgtttgct aactatctca 660 gctataaagt attgtttgaa cttt 684 // ID EH225109; SV 1; linear; mRNA; EST; PLN; 684 BP. XX AC EH225109; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5681.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5681 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-684 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; dfb4cbc24bcbd95c43df2261476c3860. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: b column: 6 CC High quality sequence stop: 609. XX FH Key Location/Qualifiers FH FT source 1..684 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5681" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 684 BP; 225 A; 147 C; 142 G; 169 T; 1 other; caaacttcct ccttctacct tggggtgttt agtatccaaa gtgattctta ggatcgagag 60 acaaccagta aatccaaaca atgaacttaa aatttagcag tttccttgtt gatggtaccg 120 tggaacagaa ttttgacaat cacaaagtgg aaggacaaaa ctcagcacct agcaaccaac 180 aatgagagac ataagatcat gattaagagc cccaaagaag cagattttcc attaaaacag 240 gagggtcatt ctcaatcaat tactgatcct gaaaaggcaa aggcttcagc gagctcagta 300 gcacaatggc caagattgaa agatccaagg attgtgcgag tatctagagc ctttggagga 360 aaagacaggc acagcaaggt tttcacaata agagggttaa gagacagacg ggtgaggcta 420 tcagtaccaa ctgccatcca actctatgat cttcaagata ggctagggct cagtcaacct 480 agcaaggttg ttgattggtt gctcgatgca gctaagcatg aaattgatga acttccacca 540 cttcctgttg ttccttcagt gaataacttc acccttggtc atccatctgc tgttacctct 600 aatgaagcta ncaactcaaa ttctcagcct aatgagcaac ttcttaatat caacagaagc 660 attcagtggg aaggttcaaa ccag 684 // ID EH225110; SV 1; linear; mRNA; EST; PLN; 445 BP. XX AC EH225110; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5847.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5847 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-445 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b882626a58e4e9dd4cec7f58194e8489. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5847.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: m column: 21 CC High quality sequence stop: 416 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..445 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5847" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 445 BP; 142 A; 79 C; 63 G; 161 T; 0 other; gaaaaaaaaa ggaattttta ttggtacatg aatataaata tattatatta caacaatgac 60 catgcatact acgcttcaat tctatctatc tatgcggcaa acataaatgc aaacaaaagt 120 aaaatttctt agctgaaaaa tagaagccat tttttttttc ttattatgga gataaatgtt 180 taaaatttaa aagcaaatca cgatacatgg cattgctatc tttcacagtc tgcccctaaa 240 ataccctctt tgtaggtttg ggatctttgg aagttgaacc aattccagaa atgcttccgg 300 tcttcttgaa gcttgatgaa cttggattcc tctggataga tttattcttc attgctgcaa 360 gattttttac ctcatgactc ttcagaaatt ctatctttgc cttcttcatt atgttactct 420 tcacatttga tacattgtat ccagg 445 // ID EH225111; SV 1; linear; mRNA; EST; PLN; 159 BP. XX AC EH225111; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5682.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5682 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-159 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 1fcce771b84b22d2aa132ab4bf066923. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5682.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0057 row: d column: 6 CC High quality sequence stop: 159. XX FH Key Location/Qualifiers FH FT source 1..159 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5682" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 159 BP; 41 A; 37 C; 18 G; 63 T; 0 other; ttgtttaact tcagtttttt ctgccgtcaa aataaataaa taaaattata ttatctattt 60 atggaaacct tcctgttcta aaacttggtc ttctcccctc aatctagctt ctccagcttc 120 gacttcgtgt ggctttccaa aagtctttcc tgcaaactt 159 // ID EH225112; SV 1; linear; mRNA; EST; PLN; 588 BP. XX AC EH225112; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6141.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6141 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-588 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; e886d8fa0f18e7754c26fecf95448740. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6141.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: j column: 24 CC High quality sequence stop: 588 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..588 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6141" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 588 BP; 175 A; 129 C; 108 G; 176 T; 0 other; ttaaaaagga aaatcacttt tacgaaccat ccacgatatt aaaccgtaaa tcaaacttta 60 agacctactt agatttcttt tggaaagatg gccaagatta ttattgccgt tatacctgct 120 gatcaggatg ctagacttaa ggcaaagtac ccaaacccac ctccagatgc caaacccgat 180 gagaaagttg ttgtagtcag cgaccctagc aaagttgttg agaccattaa ggcccagcca 240 gagcctccag ttgttgtttc cgtcaccaac tttttctctg agagcgtcca acaagaagtc 300 aagaaggccg tcgaggcagg tgccccaggt gtacctgttc tccttgtacc tcaaggatta 360 gacgacgaac aggtctacga attcttaaag gcagagaaag ccaaacattc ttagtgcttc 420 atttgttttt gatactcctt tctctgcaga tggacttaac gaataggatg ctgtcattgt 480 tacgaaaatt gaaccttttt catgtcatct tttttctttt tacaaaaaaa ctatctgagg 540 cctttataca aatgttttta cttctgtgat gcaaaaataa tttgtctc 588 // ID EH225113; SV 1; linear; mRNA; EST; PLN; 525 BP. XX AC EH225113; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ6143.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ6143 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-525 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 6999998ebcaa9aff9b337e7c96e942c9. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ6143.fwd CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0061 row: n column: 24 CC High quality sequence stop: 468 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..525 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ6143" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 525 BP; 167 A; 112 C; 116 G; 130 T; 0 other; accgaacaac acaacctata atggaataga aaaactgtag cataaagcag cttattaggc 60 attacagccc acaaatcaat gtatcaatat ctagtgcacc aaacttaatc aaaagagagg 120 ggataaactg tgctaacaga aaaacatgac aaatacgaaa taacaaagta aatagatgca 180 tatactgaaa gaaaggcggt gaacaaatca atttggtatg gaacagcctc ttacagaact 240 ccaaaatttc cctcactgcg actcttgcgc atggagagct ctgaaaccaa gttcaatgct 300 ttgtcgggag catcaaaatg atagcgactt tgggttgata ctttggttct agttggtggc 360 aggagaggct gcttaccctt ggacagtttt ggtgaatcca cacttctgcg agcatgagta 420 gacgtgattg atggcctccg tgtggctgca acaccttggc tgcccttcct tgtggtcttg 480 cgatccgctc taaggccgcg atcaaactga tgggaattca ttcct 525 // ID EH225114; SV 1; linear; mRNA; EST; PLN; 458 BP. XX AC EH225114; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5996.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5996 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-458 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 6b39c93e08c0d8f19ed60cae8ecbcd35. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5996.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more A residues CC at the end of this sequence, this clone was polyadenylated. The CC resulting Poly-A sequence has been removed. CC Small Insert: Based upon one or more sequencing reads of this clone CC where vector sequence was present at both ends, this clone has been CC determined to contain a cDNA insert on the order of 600-1000 bases. CC Plate: ABWZ 0061 row: g column: 12 CC High quality sequence stop: 458 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..458 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5996" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 458 BP; 110 A; 78 C; 107 G; 163 T; 0 other; ccactctgca tttggtgttg aggcttcgtg gaggtatgca gatatttgtg aagactctga 60 cagggaagac cattaccttg gaggtggaaa gctctgatac tattgacaat gtgaaagcta 120 agattcagga caaggaaggt atccctccag atcagcagag gctcattttt gctgggaagc 180 aattggaaga tggtcgtacc ttggcagatt acaatattca gaaggaatcc actttgcatc 240 ttgtccttcg ccttcgtggt ggttgctaag gggaatccct tttatgaagt gttctcatat 300 gtcttatctg tttgttcagt gtctcccttg aacttgtgct ttgcatctct ggtttaagtg 360 ctgctgttta ttatgtttta aaatgatgta ttgaactcgt ttcagcgaaa gtatgttgtt 420 tatgttttta ttttaaatat caatatcgtt tcacagtt 458 // ID EH225115; SV 1; linear; mRNA; EST; PLN; 570 BP. XX AC EH225115; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5847.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5847 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-570 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; cd3ada39248eba471fa18eaceef35bd2. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5847.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: m column: 21 CC High quality sequence stop: 548. XX FH Key Location/Qualifiers FH FT source 1..570 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5847" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 570 BP; 198 A; 94 C; 141 G; 135 T; 2 other; cttctttcgg atccaccttc gttgtttcga tcgaatcttg ttactatgat gagaagagat 60 caaatacgtg cacgtgagat gcgaacccca tccctggttg acctttgtgt tcagaaagta 120 atagataatg taaggtacct tggaaatgtt ggttctcttg accagcatct gctcgagcaa 180 attttgccgc actgcacagc tgaccagttg atgcatgtgg agaagtccac caagggaaga 240 aatctcagcc ctgtaactga taagttgtgg aaaaagttct atgaaaagca gtttggcaca 300 aataacacta atgaagtgat taagaggatg aaggaaaaaa gagtgaactt tagatggatg 360 caattgtatg aggcaaaagg aaaggaaagg gctcaggctg agaatgaagc acttgaccga 420 atcaggcaac tgtacaagaa agaagatgca aganaacaga gtaggcaagt aaggacatgc 480 actaaagttc caccttcaag taaaagaaga ttttggggag ataatggacc tggatacaat 540 gtatcanatg tgaaagagta cataatgaag 570 // ID EH225116; SV 1; linear; mRNA; EST; PLN; 416 BP. XX AC EH225116; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5685.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5685 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-416 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; b64b36caf60153b54701b4631ce65ca7. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: j column: 6 CC High quality sequence stop: 344. XX FH Key Location/Qualifiers FH FT source 1..416 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5685" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 416 BP; 119 A; 89 C; 91 G; 117 T; 0 other; tgttgggtac aaattggttc caggtgccac agcagctagc ctgctggatc cagaagatcc 60 tccacagaaa agagcagctt ttacaaacaa tcaaatatgg gttactcctt acaacaaaag 120 tgagcaatgg gctggtggtt tatttgctta ccagagcaaa ggggatgata ctcttcaagt 180 ctggtccaac agggatcgtc caattgagaa taaagacatt gttttgtggt acacaatagg 240 gttccatcat ataccctgtc aagaggacta tcctgtcatg cctactgtat catcaagtat 300 tgatttgaag cctgctaata tctttgagag aaatccaatc cttggagtcc ctcccaattt 360 cgaagatgat tacctgtttg caaggctcgt gattcagctt gagatcccat cagttg 416 // ID EH225117; SV 1; linear; mRNA; EST; PLN; 593 BP. XX AC EH225117; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5870.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5870 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-593 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 586cb2183a303cfa806fe4d6f743c482. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5870.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0061 row: l column: 3 CC High quality sequence stop: 491. XX FH Key Location/Qualifiers FH FT source 1..593 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5870" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 593 BP; 180 A; 95 C; 150 G; 168 T; 0 other; attggtgctc taaaggcagc caacgaacga actggacttc cagggttgat tgctgaagtg 60 aagaaggcat caccaagtag aggtatcttg agagaagact ttgacccagt tgaaattgct 120 aatgcttatg agaaaggtgg agcagcatgt ctaagtgttt tgacagatga aaagtatttt 180 aagggaagct ttgaaaatct tgaggcaata agaaaggctg gcataaagtg ccctttgttg 240 tgcaaagaat tcatcacaga tgcatggcaa ctctactatg ctcgaactaa aggtgcagat 300 gcagtccttt taattgctgc tgttttgcct gatcttgaca tcaaatacat gattaagata 360 tgcaaattac tcggattgac tgcgcttgtt gaggttcatg atgagaggga atttgatcgt 420 gttcttgcaa tagagcggat tgagcttatt ggcattaaca accgcaatct tgcttttctt 480 tttggggatt gcagacacat ttgagttgga tatcagcatc acaaagaaac ttcttgaagg 540 agagcgaggc aaaataatcc acgagagagg cataattatg gttggggaat ctg 593 // ID EH225118; SV 1; linear; mRNA; EST; PLN; 710 BP. XX AC EH225118; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5690.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5690 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-710 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 9d6d6d12ae98549f48f4c42e776eef94. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Plate: ABWZ 0057 row: d column: 8 CC High quality sequence stop: 641. XX FH Key Location/Qualifiers FH FT source 1..710 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5690" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 710 BP; 185 A; 148 C; 185 G; 192 T; 0 other; tgaatctttg gtttggttga tcacagccca cattttattg ggagacttta ctttattaaa 60 aagatcgtag tcatgccttc caatgccaag tctcgtggag gtagccgcgg tggtaggggt 120 agaggctcaa agtttcataa aggtgcctca aatcgaattc agaaggatat aagcctgtac 180 cagaccaacg gtttcaatat cgatcgacca gattcactcg tcacggccga ggatgaagat 240 gctgattttc aaaacactca aacagatggg ccatcaagcg aaattcttca gattaaattt 300 cccatagcaa tgtggggaag aagctctcta ggctaggctt gatgaccgag cttagagttg 360 gccaacgttt cagagggata gtcttaagcc ctgagggaac tatcttggtt tcggccattg 420 atcgacagtt gatcgagcag tctggaattg ctgttgtgga atgctcctgg gcaagacttg 480 aggagattcc attccaaaag ataagatcca gtggggatcg gtgcttacct tatctgatcg 540 cagccaaccc tgtcaactac ggtaagccat acaagcttac ctgcgtggag gccatcgcag 600 gtagtttggc tattgtcggt cttcaggctg aggcggttcg cttgcttgaa aaatttggat 660 ggggatcgac attctggtca ctcaatcagg atctgatcga taggtatcgt 710 // ID EH225119; SV 1; linear; mRNA; EST; PLN; 683 BP. XX AC EH225119; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5692.fwd ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5692 5', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-683 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; a5b8485aca4b7c8376a971937dd06acd. DR UNILIB; 43521; 20719. XX CC Other_ESTs: JGI_ABWZ5692.rev CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.fwd' CC indicates a forward sequencing read of the insert. CC Poly-A: Based upon evidence from another sequence read generated CC from this clone, this clone was polyadenylated. CC Plate: ABWZ 0057 row: h column: 8 CC High quality sequence stop: 626. XX FH Key Location/Qualifiers FH FT source 1..683 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5692" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-pGACTAGTTCTAGATCGCGAGCGGCCGCCC(T)15-3' 3. FT Double-stranded cDNA was ligated to Sal I adapter, digested FT with NotI and cloned into the pSPORT1 vector pre-cut with FT NotI and SalI. 4. The ligation mix was transformed into FT DH10B cells." FT /db_xref="taxon:3847" FT /db_xref="UNILIB:43521" XX SQ Sequence 683 BP; 172 A; 148 C; 133 G; 230 T; 0 other; ctcaacattt cacaggcata ccttgctttc tttgtcatca atgacctaca aatggcccaa 60 tcagccaaag ctttggttcc tgctctcata tacatctgca gcttcgttgt gtctatagca 120 ttacaggaga ttgcatggac tggccgaatg cttaaggcct actactctgc tggatgcatt 180 ctttggattt tttgtggtgc agtgatcctt cttttaacca caaatatgag ttatgtcatg 240 tacatagtat cagtattcat tggcatagca aatgctctca tgatggtgac tggagtaagc 300 atgcaaaact ttctcattgg agggaacctt aatggttgtg catttgttgt tggatcattg 360 agctttttgg acaaaatttc atgtgggatt gctttatatg ttctccagtc aaaccaaaat 420 ctctctccac agctccaggc aacaacccaa ttcccctttt cagttacaag ggttggtttg 480 ggtcttgttc ctgcattttg tgctctagtt ggggttgtag taacttgcac catggatttc 540 cacaatcctt caaagtctat gacggcacca ctattggcct agtggtgtca tttaacataa 600 tcattcacct ttctctaaca caaaagtaaa gggatatatc atacttggag aaattttagc 660 tgctctgacc cattgcgaaa ttc 683 // ID EH225120; SV 1; linear; mRNA; EST; PLN; 596 BP. XX AC EH225120; XX DT 01-JAN-2007 (Rel. 90, Created) DT 01-JAN-2007 (Rel. 90, Last updated, Version 1) XX DE JGI_ABWZ5706.rev ABWZ Phakopsora pachyrhizi infected soybean leaf tissue DE 13-15 days post inoculation with TW72-1 urediniospores Glycine max cDNA DE clone ABWZ5706 3', mRNA sequence. XX KW EST. XX OS Glycine max (soybean) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade; OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Glycine; Soja. XX RN [1] RP 1-596 RA Posada-Buitrago M.L., Brokstein P.B., Boore J.L., Frederick R.D.; RT "Comparative Analysis of expressed sequence tags in the soybean rust RT pathogen Phakopsora pachyrhizi at different stages of the infection RT process"; RL Unpublished. XX DR MD5; 3d945c0ac259ab0db969773b4eac14f2. DR UNILIB; 43521; 20719. XX CC Contact: Dr. Reid D. Frederick, Ph.D. CC USDA-ARS-NAA Foreign Disease-Weed Science Research Unit CC 1301 Ditto Avenue, Ft. Detrick, MD 21702-5023, USA CC Tel: 301 619 7386 CC Fax: 301 619 2880 CC Email: Reid.Frederick@ars.usda.gov CC cDNA Library Preparation: USDA CC DNA Sequencing: DOE Joint Genome Institute: http://www.jgi.doe.gov CC Naming Conventions: EST name is generated by the concatenation of CC the JGI Clone Id and the direction of sequencing. The suffix '.rev' CC indicates a reverse sequencing read of the insert. CC Poly-A: Based upon the presence of a run of 14 or more T residues CC at the beginning of this sequence, this clone was polyadenylated. CC The resulting Poly-T sequence has been removed. CC Plate: ABWZ 0057 row: d column: 12 CC High quality sequence stop: 592 CC POLYA=Yes. XX FH Key Location/Qualifiers FH FT source 1..596 FT /organism="Glycine max" FT /lab_host="DH10B" FT /cultivar="Williams" FT /mol_type="mRNA" FT /clone_lib="ABWZ Phakopsora pachyrhizi infected soybean FT leaf tissue 13-15 days post inoculation with TW72-1 FT urediniospores" FT /clone="ABWZ5706" FT /tissue_type="Leaf" FT /note="Vector: pSPORT1; Site_1: NotI; Site_2: SalI; 1. FT Total RNA was isolated from infected soybean leaf tissue FT 13-15 days post inoculation with P. pachyrhizi isolate FT TW72-1 using the ToTally RNA kit (Ambion, Inc., Austin, FT TX), and the poly(A)+ mRNA was purified using the OLIGOTEX FT mRNA purification kit (Qiagen, Valencia, CA). 2. First FT strand cDNA was primed with a NotI primer-adapter FT (5'-p