ID AB000556; SV 1; linear; other DNA; STD; SYN; 924 BP. XX AC AB000556; XX DT 20-JUL-1998 (Rel. 56, Created) DT 26-DEC-2006 (Rel. 90, Last updated, Version 9) XX DE Synthetic construct uncultured bacterium/plasmid NAH7 hybrid gene for DE catechol 2,3-dioxygenase, complete cds, clone P3. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Pseudomonas putida OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OG Plasmid NAH7 XX OS uncultured bacterium OC Bacteria; environmental samples. OG Plasmid NAH7 XX RN [1] RP 1-924 RA Okuta A.; RT ; RL Submitted (20-JAN-1997) to the INSDC. RL Akiko Okuta, Marine Biotechnology Institute, Kamaishi Lab.; 3-75-1 Heita, RL Kamaishi, Iwate 026, Japan (E-mail:aokuta@kamaishi.mbio.co.jp, RL Tel:0193-26-6544) XX RN [2] RX DOI; 10.1016/S0378-1119(98)00153-X. RX PUBMED; 9611265. RA Okuta A., Ohnishi K., Harayama S.; RT "PCR isolation of catechol 2,3-dioxygenase gene fragments from RT environmental samples and their assembly into functional genes"; RL Gene 212(2):221-228(1998). XX DR MD5; 0850bfeb57f2fe696826ff7de9bf22c6. XX FH Key Location/Qualifiers FH FT source 1..924 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /clone="P3" FT /db_xref="taxon:32630" FT source 1..339 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT source 340..808 FT /organism="uncultured bacterium" FT /environmental_sample FT /mol_type="other DNA" FT /note="isolated from a mixed culture of phenol-degrading FT bacteria" FT /db_xref="taxon:77133" FT source 809..924 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT CDS 1..924 FT /codon_start=1 FT /transl_table=11 FT /product="catechol 2,3-dioxygenase" FT /protein_id="BAA31715.1" FT /translation="MNKGVMRPGHVQLRVLDMGKALEHYVELLGLIEMDRDDQGRVYLK FT AWTEVDKFSVVLREADEPGMDFMGFKVVDEDSLNRLTDDLLNFGCLIENVAAGELKGCG FT RRVRFQAPSGHHFELYADKEYTGKWGIEEVNPEAWPRDLKGMRAVRFDHCLMYGDELQA FT TYELFTEVLGFYLAEQVIEDNGTRISQFLSLSTKAHDVAFIQHAEKGKFHHVSFFLETW FT EDVLRAADLISMTDTSIDIGPTRHGLTHGKTMYFFDPSGNRCEVFCGGNYNYPDHKPVT FT WLAKDVGKAIFYHDRVLNERFMTVMT" XX SQ Sequence 924 BP; 202 A; 258 C; 269 G; 195 T; 0 other; atgaacaaag gtgtaatgcg ccccggccac gtgcaactgc gtgtactgga catgggcaag 60 gccttggaac actacgtcga gttgcttggc ctgatcgaga tggatcgtga cgaccaaggc 120 cgtgtctatc tgaaggcctg gactgaggtt gacaaattct ccgtggtgct gcgcgaagcc 180 gatgagccag gtatggattt tatgggtttc aaggtggtcg acgaagatag tctaaatcgc 240 ctcacggatg acctgctcaa ctttggctgt ctgatagaaa acgtcgccgc aggagaactc 300 aaagggtgtg gtcgccgcgt gcgcttccag gccccctctg ggcatcactt cgagttgtat 360 gctgacaagg agtacactgg taaatggggc atcgaggagg tcaaccctga agcctggcca 420 cgtgacctga agggtatgcg cgcggtgcgc ttcgaccact gtctgatgta cggcgacgaa 480 ctgcaggcga cctacgaact gttcactgag gtgctcggtt tctacctggc cgagcaggtg 540 atcgaagaca acggcacgcg catctcccag ttcctcagcc tgtcgaccaa ggcgcacgac 600 gtggccttta tccagcacgc ggagaagggc aagttccatc acgtctcatt cttcctggaa 660 acctgggaag acgtgcttcg cgccgccgac ctgatctcca tgaccgacac ctcgatcgat 720 atcggcccga cccgtcacgg tctgacccac ggcaagacca tgtacttctt cgacccctcc 780 ggcaaccgct gcgaggtgtt ctgcggcggg aattacaact atccggatca taagccggtg 840 acttggttgg ccaaggatgt gggcaaggcg atcttctatc acgaccgggt gctcaacgaa 900 cgattcatga ccgttatgac ctaa 924 // ID AB000557; SV 1; linear; other DNA; STD; SYN; 924 BP. XX AC AB000557; XX DT 20-JUL-1998 (Rel. 56, Created) DT 26-DEC-2006 (Rel. 90, Last updated, Version 9) XX DE Synthetic construct uncultured bacterium/plasmid NAH7 hybrid gene for DE catechol 2,3-dioxygenase, complete cds, clone P8. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Pseudomonas putida OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OG Plasmid NAH7 XX OS uncultured bacterium OC Bacteria; environmental samples. OG Plasmid NAH7 XX RN [1] RP 1-924 RA Okuta A.; RT ; RL Submitted (20-JAN-1997) to the INSDC. RL Akiko Okuta, Marine Biotechnology Institute, Kamaishi Lab.; 3-75-1 Heita, RL Kamaishi, Iwate 026, Japan (E-mail:aokuta@kamaishi.mbio.co.jp, RL Tel:0193-26-6544) XX RN [2] RX DOI; 10.1016/S0378-1119(98)00153-X. RX PUBMED; 9611265. RA Okuta A., Ohnishi K., Harayama S.; RT "PCR isolation of catechol 2,3-dioxygenase gene fragments from RT environmental samples and their assembly into functional genes"; RL Gene 212(2):221-228(1998). XX DR MD5; 145f75bda8a822d8ab994854477f5461. XX FH Key Location/Qualifiers FH FT source 1..924 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /clone="P8" FT /db_xref="taxon:32630" FT source 1..339 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT source 340..808 FT /organism="uncultured bacterium" FT /environmental_sample FT /mol_type="other DNA" FT /note="isolated from a mixed culture of phenol-degrading FT bacteria" FT /db_xref="taxon:77133" FT source 809..924 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT CDS 1..924 FT /codon_start=1 FT /transl_table=11 FT /product="catechol 2,3-dioxygenase" FT /protein_id="BAA31716.1" FT /translation="MNKGVMRPGHVQLRVLDMGKALEHYVELLGLIEMDRDDQGRVYLK FT AWTEVDKFSVVLREADEPGMDFMGFKVVDEDSLNRLTDDLLNFGCLIENVAAGELKGCG FT RRVRFQAPSGHHFELYADKEYTGKWGVNEVNPEAWPRDLKGMAAVRFDHALMYGDELPA FT TYDLFTKVLGFYLAERVLDENGTRVAQFLSLSTKAHDVAFIHHPEKGRLHHVSFHLETW FT EDVLRAADLISMTDTSIDISPTRHGLTHGKTMYFFDPSGNRCEVFCGGNYNYPDHKPVT FT WLAKDVGKAIFYHDRVLNERFMTVMT" XX SQ Sequence 924 BP; 203 A; 261 C; 263 G; 197 T; 0 other; atgaacaaag gtgtaatgcg ccccggccac gtgcaactgc gtgtactgga catgggcaag 60 gccttggaac actacgtcga gttgcttggc ctgatcgaga tggatcgtga cgaccaaggc 120 cgtgtctatc tgaaggcctg gactgaggtt gacaaattct ccgtggtgct gcgcgaagcc 180 gatgagccag gtatggattt tatgggtttc aaggtggtcg acgaagatag tctaaatcgc 240 ctcacggatg acctgctcaa ctttggctgt ctgatagaaa acgtcgccgc aggagaactc 300 aaagggtgtg gtcgccgcgt gcgcttccag gccccctctg ggcatcactt cgagttgtat 360 gctgacaagg agtacaccgg taaatggggt gtgaatgagg tcaatcccga ggcatggccg 420 cgcgatctga aaggtatggc ggctgtgcgt ttcgaccacg ccctcatgta tggcgacgaa 480 ttgccggcga cctatgacct gttcaccaag gtgctcggct tctatctggc cgaacgggtg 540 ctggacgaaa atggcacgcg cgtcgcccag ttcctcagtc tgtcgaccaa ggcccacgac 600 gtggccttca ttcaccatcc ggaaaaaggc cgcctccatc atgtgtcctt ccacctcgaa 660 acctgggaag acgtgcttcg cgccgccgac ctgatctcca tgaccgacac ctcgatcgac 720 atcagcccaa cccgccacgg cctcactcac ggcaagacaa tgtacttctt tgacccatcc 780 ggcaaccgct gcgaggtgtt ctgcggcggg aattacaact atccggatca taagccggtg 840 acttggttgg ccaaggatgt gggcaaggcg atcttctatc acgaccgggt gctcaacgaa 900 cgattcatga ccgttatgac ctaa 924 // ID AB000558; SV 1; linear; other DNA; STD; SYN; 924 BP. XX AC AB000558; XX DT 20-JUL-1998 (Rel. 56, Created) DT 26-DEC-2006 (Rel. 90, Last updated, Version 9) XX DE Synthetic construct uncultured bacterium/plasmid NAH7 hybrid gene for DE catechol 2,3-dioxygenase, complete cds, clone P16. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Pseudomonas putida OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OG Plasmid NAH7 XX OS uncultured bacterium OC Bacteria; environmental samples. OG Plasmid NAH7 XX RN [1] RP 1-924 RA Okuta A.; RT ; RL Submitted (20-JAN-1997) to the INSDC. RL Akiko Okuta, Marine Biotechnology Institute, Kamaishi Lab.; 3-75-1 Heita, RL Kamaishi, Iwate 026, Japan (E-mail:aokuta@kamaishi.mbio.co.jp, RL Tel:0193-26-6544) XX RN [2] RX DOI; 10.1016/S0378-1119(98)00153-X. RX PUBMED; 9611265. RA Okuta A., Ohnishi K., Harayama S.; RT "PCR isolation of catechol 2,3-dioxygenase gene fragments from RT environmental samples and their assembly into functional genes"; RL Gene 212(2):221-228(1998). XX DR MD5; 27d0bda355367b0ba8e8a4c148c2a109. XX FH Key Location/Qualifiers FH FT source 1..924 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /clone="P16" FT /db_xref="taxon:32630" FT source 1..339 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT source 340..808 FT /organism="uncultured bacterium" FT /environmental_sample FT /mol_type="other DNA" FT /note="isolated from a mixed culture of phenol-degrading FT bacteria" FT /db_xref="taxon:77133" FT source 809..924 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT CDS 1..924 FT /codon_start=1 FT /transl_table=11 FT /product="catechol 2,3-dioxygenase" FT /protein_id="BAA31717.1" FT /translation="MNKGVMRPGHVQLRVLDMGKALEHYVELLGLIEMDRDDQGRVYLK FT AWTEVDKFSVVLREADEPGMDFMGFKVVDEDSLNRLTDDLLNFGCLIENVAAGELKGCG FT RRVRFQAPSGHHFELYADKEYTGKWGVSDFNPEAWPRGLKGMRAVRFDHCLLYGGELQA FT TYDLLVNVLGFYLAEQVVDDQGNRVAQFMSLATKAHDVAFIQHAEPGRLHHVSFYLETW FT DDVLRAADLISMTNSSLDIGPTRHGLTHGKTIYFFDPSGNRCEVFCGGNYNYPDHKPVT FT WLAKDVGKAIFYHDRVLNERFMTVMT" XX SQ Sequence 924 BP; 188 A; 269 C; 277 G; 190 T; 0 other; atgaacaaag gtgtaatgcg ccccggccac gtgcaactgc gtgtactgga catgggcaag 60 gccttggaac actacgtcga gttgcttggc ctgatcgaga tggatcgtga cgaccaaggc 120 cgtgtctatc tgaaggcctg gactgaggtt gacaaattct ccgtggtgct gcgcgaagcc 180 gatgagccag gtatggattt tatgggtttc aaggtggtcg acgaagatag tctaaatcgc 240 ctcacggatg acctgctcaa ctttggctgt ctgatagaaa acgtcgccgc aggagaactc 300 aaagggtgtg gtcgccgcgt gcgcttccag gccccctctg ggcatcactt cgagttgtat 360 gctgacaagg aatatacggg gaaatggggc gtctcggact tcaacccgga agcctggccc 420 cggggcctca agggcatgcg tgcggtgcgc ttcgaccatt gcctgctgta cggcggcgag 480 ttgcaggcca cctacgacct gctggtcaac gtgctcggct tctacctggc cgagcaggtg 540 gtggacgacc agggcaaccg cgtcgcccag ttcatgagcc tggccaccaa ggcccacgat 600 gtcgccttca tccagcatgc cgagccgggt cgcctgcacc acgtgtcgtt ctacctggaa 660 acctgggacg acgtgctgcg cgccgccgac ctgatttcca tgaccaactc ctcgctggac 720 atcggcccga cccgccacgg cctgacccac ggcaagacaa tttacttctt tgacccgtcc 780 ggcaaccgct gcgaggtgtt ctgcggcggg aattacaact atccggatca taagccggtg 840 acttggttgg ccaaggatgt gggcaaggcg atcttctatc acgaccgggt gctcaacgaa 900 cgattcatga ccgttatgac ctaa 924 // ID AB000559; SV 1; linear; other DNA; STD; SYN; 924 BP. XX AC AB000559; XX DT 20-JUL-1998 (Rel. 56, Created) DT 26-DEC-2006 (Rel. 90, Last updated, Version 9) XX DE Synthetic construct uncultured bacterium/plasmid NAH7 hybrid gene for DE catechol 2,3-dioxygenase, complete cds, clone O1. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Pseudomonas putida OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OG Plasmid NAH7 XX OS uncultured bacterium OC Bacteria; environmental samples. OG Plasmid NAH7 XX RN [1] RP 1-924 RA Okuta A.; RT ; RL Submitted (20-JAN-1997) to the INSDC. RL Akiko Okuta, Marine Biotechnology Institute, Kamaishi Lab.; 3-75-1 Heita, RL Kamaishi, Iwate 026, Japan (E-mail:aokuta@kamaishi.mbio.co.jp, RL Tel:0193-26-6544) XX RN [2] RX DOI; 10.1016/S0378-1119(98)00153-X. RX PUBMED; 9611265. RA Okuta A., Ohnishi K., Harayama S.; RT "PCR isolation of catechol 2,3-dioxygenase gene fragments from RT environmental samples and their assembly into functional genes"; RL Gene 212(2):221-228(1998). XX DR MD5; 2d9b64b5b93916bcd1cc577296f82f66. XX FH Key Location/Qualifiers FH FT source 1..924 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /clone="O1" FT /db_xref="taxon:32630" FT source 1..339 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT source 340..808 FT /organism="uncultured bacterium" FT /environmental_sample FT /mol_type="other DNA" FT /note="isolated from a mixed culture of crude oil-degrading FT bacteria" FT /db_xref="taxon:77133" FT source 809..924 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT CDS 1..924 FT /codon_start=1 FT /transl_table=11 FT /product="catechol 2,3-dioxygenase" FT /protein_id="BAA31718.1" FT /translation="MNKGVMRPGHVQLRVLDMGKALEHYVELLGLIEMDRDDQGRVYLK FT AWTEVDKFSVVLREADEPGMDFMGFKVVDEDSLNRLTDDLLNFGCLIENVAAGELKGCG FT RRVRFQAPSGHYFEWSAEKEYTGKWGLEEIYPEAWPRNLKGMRAVRFDHCLLYGDELQA FT TYALFTEVLGFYLAEQVVDDNGTRIAQFLSLSTKAHDVAFIQHTEKGRFHHASFFLETW FT EDVLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNQCLVGRGGNYNYPDHKPVT FT WLAKDVGKAIFYHDRVLNERFMTVMT" XX SQ Sequence 924 BP; 198 A; 262 C; 275 G; 189 T; 0 other; atgaacaaag gtgtaatgcg ccccggccac gtgcaactgc gtgtactgga catgggcaag 60 gccttggaac actacgtcga gttgcttggc ctgatcgaga tggatcgtga cgaccaaggc 120 cgtgtctatc tgaaggcctg gactgaggtt gacaaattct ccgtggtgct gcgcgaagcc 180 gatgagccag gtatggattt tatgggtttc aaggtggtcg acgaagatag tctaaatcgc 240 ctcacggatg acctgctcaa ctttggctgt ctgatagaaa acgtcgccgc aggagaactc 300 aaagggtgtg gtcgccgcgt gcgcttccag gccccctctg ggcattactt cgagtggtct 360 gctgaaaagg aatatacggg taagtggggg ctggaggaga tctatccgga agcctggccg 420 cgcaacctca agggcatgcg cgcggtgcgc ttcgaccact gcctgctgta tggcgacgag 480 ctgcaggcga cctatgcgct gttcaccgag gtgctcggtt tctacctggc cgaacaggtg 540 gtcgacgaca acggcacgcg catcgcgcag ttcctcagcc tgtcgaccaa ggcccacgac 600 gtggccttca tccagcacac ggagaagggc cgcttccacc acgcctcgtt ctttctggaa 660 acctgggaag acgtgctgcg cgcagccgac ctgatttcca tgaccgacac ctcgatcgac 720 atcggcccga cccgccacgg cctgacccat ggcaagacaa tatatttctt cgacccctcc 780 ggcaaccaat gcttggtggg ccgcggcggg aattacaact atccggatca taagccggtg 840 acttggttgg ccaaggatgt gggcaaggcg atcttctatc acgaccgggt gctcaacgaa 900 cgattcatga ccgttatgac ctaa 924 // ID AB000560; SV 1; linear; other DNA; STD; SYN; 924 BP. XX AC AB000560; XX DT 20-JUL-1998 (Rel. 56, Created) DT 26-DEC-2006 (Rel. 90, Last updated, Version 9) XX DE Synthetic construct uncultured bacterium/plasmid NAH7 hybrid gene for DE catechol 2,3-dioxygenase, complete cds, clone O2. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Pseudomonas putida OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OG Plasmid NAH7 XX OS uncultured bacterium OC Bacteria; environmental samples. OG Plasmid NAH7 XX RN [1] RP 1-924 RA Okuta A.; RT ; RL Submitted (20-JAN-1997) to the INSDC. RL Akiko Okuta, Marine Biotechnology Institute, Kamaishi Lab.; 3-75-1 Heita, RL Kamaishi, Iwate 026, Japan (E-mail:aokuta@kamaishi.mbio.co.jp, RL Tel:0193-26-6544) XX RN [2] RX DOI; 10.1016/S0378-1119(98)00153-X. RX PUBMED; 9611265. RA Okuta A., Ohnishi K., Harayama S.; RT "PCR isolation of catechol 2,3-dioxygenase gene fragments from RT environmental samples and their assembly into functional genes"; RL Gene 212(2):221-228(1998). XX DR MD5; 7bc3dcbf6b23d67259eff284ef1dbb6c. XX FH Key Location/Qualifiers FH FT source 1..924 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /clone="O2" FT /db_xref="taxon:32630" FT source 1..339 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT source 340..808 FT /organism="uncultured bacterium" FT /environmental_sample FT /mol_type="other DNA" FT /note="isolated from a mixed culture of crude oil-degrading FT bacteria" FT /db_xref="taxon:77133" FT source 809..924 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT CDS 1..924 FT /codon_start=1 FT /transl_table=11 FT /product="catechol 2,3-dioxygenase" FT /protein_id="BAA31719.1" FT /translation="MNKGVMRPGHVQLRVLDMGKALEHYVELLGLIEMDRDDQGRVYLK FT AWTEVDKFSVVLREADEPGMDFMGFKVVDEDSLNRLTDDLLNFGCLIENVAAGELKGCG FT RRVRFQAPSGHYFEWSADKEYTGKWGLEEINPEAWPRNLKGMRAVRFDHCLLYGDELQA FT TYALFTEVLGFYLAEQVVDDNGTRIAQFLSLSTKAHDVAFIQHTEKGRFHHASFFLETW FT EDLLRAADLISMTDTSIDIGPTRHGLTHGKTMYFFDPSGNQCVVGPRRNYNYPDHKPVT FT WLAKDVGKAIFYHDRVLNERFMTVMT" XX SQ Sequence 924 BP; 200 A; 264 C; 273 G; 187 T; 0 other; atgaacaaag gtgtaatgcg ccccggccac gtgcaactgc gtgtactgga catgggcaag 60 gccttggaac actacgtcga gttgcttggc ctgatcgaga tggatcgtga cgaccaaggc 120 cgtgtctatc tgaaggcctg gactgaggtt gacaaattct ccgtggtgct gcgcgaagcc 180 gatgagccag gtatggattt tatgggtttc aaggtggtcg acgaagatag tctaaatcgc 240 ctcacggatg acctgctcaa ctttggctgt ctgatagaaa acgtcgccgc aggagaactc 300 aaagggtgtg gtcgccgcgt gcgcttccag gccccctctg ggcattactt cgagtggtct 360 gctgacaaag aatacaccgg caagtggggg ctggaggaaa tcaatccgga agcctggccg 420 cgcaacctca agggcatgcg cgcggtgcgc ttcgaccact gcctgctgta tggcgacgag 480 ctgcaggcga cctatgcgct gttcaccgag gtgctcggtt tctacctggc cgaacaggtg 540 gtcgacgaca acggcacgcg catcgcgcag ttcctcagcc tgtcgaccaa ggcccacgac 600 gtggccttca ttcagcacac ggagaagggc cgcttccacc acgcctcgtt ctttctggaa 660 acctgggaag acctgctgcg cgcagccgac ctgatttcca tgaccgacac ctcgatcgac 720 atcggcccga cccgccacgg cctgacccat ggcaagacaa tgtacttctt tgatccatcc 780 ggcaaccaat gcgtggtggg gccgcggcgg aattacaact atccggatca taagccggtg 840 acttggttgg ccaaggatgt gggcaaggcg atcttctatc acgaccgggt gctcaacgaa 900 cgattcatga ccgttatgac ctaa 924 // ID AB000561; SV 1; linear; other DNA; STD; SYN; 924 BP. XX AC AB000561; XX DT 20-JUL-1998 (Rel. 56, Created) DT 26-DEC-2006 (Rel. 90, Last updated, Version 9) XX DE Synthetic construct uncultured bacterium/plasmid NAH7 hybrid gene for DE catechol 2,3-dioxygenase, complete cds, clone O12. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Pseudomonas putida OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Pseudomonadaceae; Pseudomonas. OG Plasmid NAH7 XX OS uncultured bacterium OC Bacteria; environmental samples. OG Plasmid NAH7 XX RN [1] RP 1-924 RA Okuta A.; RT ; RL Submitted (20-JAN-1997) to the INSDC. RL Akiko Okuta, Marine Biotechnology Institute, Kamaishi Lab.; 3-75-1 Heita, RL Kamaishi, Iwate 026, Japan (E-mail:aokuta@kamaishi.mbio.co.jp, RL Tel:0193-26-6544) XX RN [2] RX DOI; 10.1016/S0378-1119(98)00153-X. RX PUBMED; 9611265. RA Okuta A., Ohnishi K., Harayama S.; RT "PCR isolation of catechol 2,3-dioxygenase gene fragments from RT environmental samples and their assembly into functional genes"; RL Gene 212(2):221-228(1998). XX DR MD5; f8555f9e41167f47b6eccbaa1212d425. XX FH Key Location/Qualifiers FH FT source 1..924 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /clone="O12" FT /db_xref="taxon:32630" FT source 1..339 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT source 340..808 FT /organism="uncultured bacterium" FT /environmental_sample FT /mol_type="other DNA" FT /note="isolated from a mixed culture of crude oil-degrading FT bacteria" FT /db_xref="taxon:77133" FT source 809..924 FT /organism="Pseudomonas putida" FT /plasmid="NAH7" FT /mol_type="other DNA" FT /db_xref="taxon:303" FT CDS 1..924 FT /codon_start=1 FT /transl_table=11 FT /product="catechol 2,3-dioxygenase" FT /protein_id="BAA31720.1" FT /translation="MNKGVMRPGHVQLRVLDMGKALEHYVELLGLIEMDRDDQGRVYLK FT AWTEVDKFSVVLREADEPGMDFMGFKVVDEDSLNRLTDDLLNFGCLIENVAAGELKGCG FT RRVRFQAPSGHHFEWSADKEYTGKWGVGNRNPEAWPRGLQGMKATRFDHCLLYGTDLDR FT TLELFRDVLGFDLAEQVVTPDGKRVAQFLTASMKAHDVAFIDHPEPGKFHHASFFLETW FT EDVLRAADLISMTDTSIDIGPTRHGLTHGKTIYFFDPSGNQCVVGRGGNYNYPDHKPVT FT WLAKDVGKAIFYHDRVLNERFMTVMT" XX SQ Sequence 924 BP; 199 A; 252 C; 279 G; 194 T; 0 other; atgaacaaag gtgtaatgcg ccccggccac gtgcaactgc gtgtactgga catgggcaag 60 gccttggaac actacgtcga gttgcttggc ctgatcgaga tggatcgtga cgaccaaggc 120 cgtgtctatc tgaaggcctg gactgaggtt gacaaattct ccgtggtgct gcgcgaagcc 180 gatgagccag gtatggattt tatgggtttc aaggtggtcg acgaagatag tctaaatcgc 240 ctcacggatg acctgctcaa ctttggctgt ctgatagaaa acgtcgccgc aggagaactc 300 aaagggtgtg gtcgccgcgt gcgcttccag gccccctctg ggcatcactt cgagtggtct 360 gctgacaagg aatatactgg aaagtggggc gtgggcaacc gcaaccccga agcctggccg 420 cgcggcctgc agggcatgaa ggcgacgcgc tttgaccact gcctgctcta tggcactgat 480 ctggatcgca ccctggagct gttccgggac gtgctgggat tcgatctggc cgagcaggtg 540 gtaaccccgg atggcaagcg agtggcgcag ttcctgacag ccagcatgaa agcccatgat 600 gtggccttca tcgatcatcc ggaaccgggc aagttccacc acgcctcctt cttcctggaa 660 acctgggaag acgtgctgcg tgcggcggac ctgatctcga tgaccgatac ctccatcgat 720 atcggcccga cccgtcatgg cttgacccat ggcaagacga tatatttctt cgatccgtcc 780 ggcaaccaat gcgtggtagg ccgcggcggg aattacaact atccggatca taagccggtg 840 acttggttgg ccaaggatgt gggcaaggcg atcttctatc acgaccgggt gctcaacgaa 900 cgattcatga ccgttatgac ctaa 924 // ID AB001326; SV 1; circular; other DNA; STD; SYN; 3930 BP. XX AC AB001326; XX DT 04-APR-1997 (Rel. 51, Created) DT 20-FEB-2009 (Rel. 99, Last updated, Version 4) XX DE Cloning vector pBEN77 genes for chloramphenicol-acetyltransferase, DE beta-lactamase, complete cds. XX KW . XX OS Cloning vector pBEN77 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3930 RA Yamamoto Y.; RT ; RL Submitted (20-FEB-1997) to the INSDC. RL Contact:Yoshihiro Yamamoto Hyogo College of Medicine, Department of RL Genetics; Mukogawa-cho 1-1, Nishinomiya, Hyogo 663, Japan XX RN [2] RA Yamamoto Y., Tamura K., Furuyama J.; RT "Disruption of Escherichia coli genes by circle DNA insertion"; RL Unpublished. XX DR MD5; 235b9fb19c608ac74c80f41df632a7de. XX FH Key Location/Qualifiers FH FT source 1..3930 FT /organism="Cloning vector pBEN77" FT /lab_host="Escherichia coli" FT /mol_type="other DNA" FT /db_xref="taxon:58105" FT regulatory complement(87..311) FT /note="lactose operon fragment (including lac promoter)" FT /regulatory_class="other" FT CDS complement(902..1561) FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="chloramphenicol-acetyltransferase" FT /note="chloramphenicol resistance gene" FT /note="derived from Tn9" FT /protein_id="BAA19238.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS complement(2833..3693) FT /codon_start=1 FT /transl_table=11 FT /gene="amp" FT /product="beta-lactamase" FT /note="ampicillin resistance gene" FT /protein_id="BAA19239.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" XX SQ Sequence 3930 BP; 999 A; 1011 C; 924 G; 996 T; 0 other; gcggccgcgc gcgcgcaatt aaccctcact aaagggaaca aaagctgggc gactctagag 60 gatccccggg taccgagctc gaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat 120 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg 180 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag 240 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt 300 ttgcgtattg gaacgccatg agcggcctca tttcttattc tgagttacaa cagtccgcac 360 cgctgtccgg tagctccttc cggtgggcgc ggggcatgac tatcgtcgcc gcacttatga 420 ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc agcgcccaac agtcccccgg 480 ccacggggcc tgccaccata cccacgccga aacaagcgcc ctgcaccatt atgttccgga 540 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc 600 gctaaccgtt tttatcaggc tctgggaggc agaataaatg atcatatcgt caattattac 660 ctccacgggg agagcctgag caaactggcc tcaggcattt gagaagcaca cggtcacact 720 gcttccggta gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc 780 ctgccctgaa ccgacgaccg ggtcgaattt gctttcgaat ttctgccatt catccgctta 840 ttatcactta ttcaggcgta gcaccaggcg tttaagggca ccaataactg ccttaaaaaa 900 attacgcccc gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga 960 catggaagcc atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt 1020 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg 1080 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat 1140 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg 1200 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg 1260 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct 1320 caccgtcttt cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt 1380 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa 1440 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat 1500 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca 1560 ttttagcttc cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc 1620 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc 1680 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga 1740 agtgatcttc cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact 1800 tactgattta gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc 1860 tgtccctcct gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat 1920 cagcgctagc ggagtgtatc ctggtatgag tcagcaacac cttcttcacg aggcagacct 1980 cagcgccccc cccccccggc cgccaccgcg cggccgctga gcaaaaggcc agcaaaaggc 2040 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 2100 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 2160 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 2220 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 2280 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 2340 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 2400 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 2460 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 2520 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 2580 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 2640 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 2700 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 2760 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 2820 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 2880 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 2940 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 3000 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 3060 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 3120 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 3180 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 3240 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 3300 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 3360 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 3420 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 3480 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 3540 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 3600 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 3660 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 3720 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 3780 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 3840 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 3900 tttcggtgat gacggtgaaa acctctgaca 3930 // ID AB001897; SV 1; linear; other DNA; STD; SYN; 3200 BP. XX AC AB001897; XX DT 04-APR-1997 (Rel. 51, Created) DT 15-SEP-2006 (Rel. 89, Last updated, Version 4) XX DE Cloning vector pBEN70 plasmid pBEN70 DNA for chloramphenicol DE acetyltransferase, beta-lactamase, complete cds. XX KW . XX OS Cloning vector pBEN70 OC other sequences; artificial sequences; vectors. OG Plasmid pBEN70 XX RN [1] RP 1-3200 RA Yamamoto Y.; RT ; RL Submitted (18-MAR-1997) to the INSDC. RL Yoshihiro Yamamoto, Hyogo College of Medicine, Department of Genetics; RL Mukogawa-cho, Nishinomiya, Hyogo 663, Japan (E-mail:yosyam@hyo-med.ac.jp, RL Tel:81-798-45-6587, Fax:81-798-40-7639) XX RN [2] RA Yamamoto Y., Tamura K., Furuyama J.; RT "Disruption of Escherichia coli genes by circle DNA insertion"; RL Unpublished. XX DR MD5; 50803d646ecfa69a1d1fe2c00d28e6fb. XX FH Key Location/Qualifiers FH FT source 1..3200 FT /organism="Cloning vector pBEN70" FT /plasmid="pBEN70" FT /mol_type="other DNA" FT /db_xref="taxon:58104" FT regulatory 19..36 FT /note="T3 promotor" FT /regulatory_class="promoter" FT misc_feature 54..86 FT /note="multiple cloning site ; FT (XbaI-BamHI-SmaI-KpnI-SacI-EcoRI)" FT CDS complement(172..831) FT /codon_start=1 FT /transl_table=11 FT /gene="cat-1" FT /product="chloramphenicol acetyltransferase" FT /note="chloramphenicol resistant gene" FT /protein_id="BAA19464.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS complement(2103..2963) FT /codon_start=1 FT /transl_table=11 FT /standard_name="ampicillin resistant gene" FT /product="beta-lactamase" FT /protein_id="BAA19465.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" XX SQ Sequence 3200 BP; 826 A; 804 C; 743 G; 827 T; 0 other; gcggccgcgc gcgcgcaatt aaccctcact aaagggaaca aaagctgggc gactctagag 60 gatccccggg taccgagctc gaattcgaat ttctgccatt catccgctta ttatcactta 120 ttcaggcgta gcaccaggcg tttaagggca ccaataactg ccttaaaaaa attacgcccc 180 gccctgccac tcatcgcagt actgttgtaa ttcattaagc attctgccga catggaagcc 240 atcacagacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt cgccttgcgt 300 ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg ccacgtttaa 360 atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat tctcaataaa 420 ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg aatatatgtg 480 tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg tttcagtttg 540 ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct caccgtcttt 600 cattgccata cgaaattccg gatgagcatt catcaggcgg gcaagaatgt gaataaaggc 660 cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa tatccagctg 720 aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat gttctttacg 780 atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca ttttagcttc 840 cttagctcct gaaaatctcg ataactcaaa aaatacgccc ggtagtgatc ttatttcatt 900 atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc aaaagttggc 960 ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga agtgatcttc 1020 cgtcacaggt atttattcgg cgcaaagtgc gtcgggtgat gctgccaact tactgattta 1080 gtgtatgatg gtgtttttga ggtgctccag tggcttctgt ttctatcagc tgtccctcct 1140 gttcagctac tgacggggtg gtgcgtaacg gcaaaagcac cgccggacat cagcgctagc 1200 ggagtgtatc ctggtatgag tcagcaacac cttcttcacg aggcagacct cagcgccccc 1260 cccccccggc cgccaccgcg cggccgctga gcaaaaggcc agcaaaaggc caggaaccgt 1320 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 1380 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 1440 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 1500 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 1560 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 1620 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 1680 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 1740 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 1800 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 1860 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 1920 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 1980 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 2040 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 2100 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 2160 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 2220 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 2280 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 2340 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 2400 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 2460 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 2520 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 2580 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 2640 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 2700 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 2760 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 2820 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 2880 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 2940 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 3000 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 3060 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 3120 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat 3180 gacggtgaaa acctctgaca 3200 // ID AB003468; SV 1; circular; other DNA; STD; SYN; 5350 BP. XX AC AB003468; XX DT 02-DEC-1997 (Rel. 53, Created) DT 19-NOV-2004 (Rel. 81, Last updated, Version 4) XX DE Cloning vector pAP3neo DNA, complete sequence. XX KW Amp; beta-lactamase; neo; neomycin phosphotransferase. XX OS Cloning vector pAP3neo OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-5350 RA Nojima H.; RT ; RL Submitted (30-MAY-1997) to the INSDC. RL Hiroshi Nojima, Research Institute for Microbial Diseases, Osaka RL University, Department of Molecular Genetics; Yamadaoka 3-1, Suita, Osaka, RL 565-0871, Japan (E-mail:snj-0212@biken.osaka-u.ac.jp, Tel:81-6-6875-3980, RL Fax:81-6-6875-5192) XX RN [2] RX DOI; 10.1046/j.1365-2443.1998.00202.x. RX PUBMED; 9753427. RA Kobori M., Ikeda Y., Nara H., Kato M., Kumegawa M., Nojima H., RA Kawashima H.; RT "Large scale isolation of osteoclast-specific genes by an improved method RT involving the preparation of a subtracted cDNA library"; RL Genes Cells 3(7):459-475(1998). XX DR MD5; 82815632263cad694127fd216b1ede2d. XX FH Key Location/Qualifiers FH FT source 1..5350 FT /organism="Cloning vector pAP3neo" FT /mol_type="other DNA" FT /db_xref="taxon:69403" FT regulatory 47..268 FT /note="SV40 late polyadenylation signal" FT /regulatory_class="polyA_signal_sequence" FT rep_origin 572..1452 FT /note="ColE1-derived plasmid replication origin" FT CDS complement(1513..2373) FT /codon_start=1 FT /transl_table=11 FT /gene="Amp" FT /product="beta-lactamase" FT /protein_id="BAA23652.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT rep_origin 2504..2959 FT /note="phage f1 region" FT regulatory 3290..3485 FT /note="SV40 early promoter" FT /regulatory_class="promoter" FT CDS 3596..4390 FT /codon_start=1 FT /transl_table=11 FT /gene="neo" FT /product="neomycin phosphotransferase" FT /protein_id="BAA23653.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT regulatory 4446..4638 FT /note="SV40 late polyadenylation signal" FT /regulatory_class="polyA_signal_sequence" FT regulatory 4788..4983 FT /note="SV40 early promoter" FT /regulatory_class="promoter" FT regulatory 5188..5207 FT /note="T7 promoter" FT /regulatory_class="promoter" FT regulatory 5302..5321 FT /note="T3 promoter" FT /regulatory_class="promoter" XX SQ Sequence 5350 BP; 1321 A; 1324 C; 1405 G; 1300 T; 0 other; ggtaccttct gaggcggaaa gaaccagccg gatccctcga gggatccaga catgataaga 60 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 120 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 180 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 240 agcaagtaaa acctctacaa atgtggtatg gctgattatg atccggctgc ctcgcgcgtt 300 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 360 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 420 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 480 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag 540 atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct 600 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 660 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 720 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 780 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 840 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 900 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 960 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 1020 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 1080 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 1140 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt 1200 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 1260 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 1320 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 1380 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 1440 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 1500 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 1560 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 1620 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 1680 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 1740 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 1800 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 1860 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 1920 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 1980 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 2040 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 2100 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 2160 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 2220 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 2280 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 2340 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 2400 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 2460 acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat 2520 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 2580 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 2640 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 2700 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 2760 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 2820 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 2880 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 2940 gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 3000 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 3060 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 3120 cgcgtcgacg gtatcgataa gcttggctgt ggaatgtgtg tcagttaggg tgtggaaagt 3180 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 3240 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 3300 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt 3360 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 3420 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt 3480 gcaaaaagct cctcgaggaa ctgaaaaacc agaaagttaa ctggtaagtt tagtcttttt 3540 gtcttttatt tcaggtcccg gatctgatca agagacagga tgaggatcgt ttcgcatgat 3600 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta 3660 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca 3720 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga 3780 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga 3840 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct 3900 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg 3960 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga 4020 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca 4080 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga 4140 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg 4200 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc 4260 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt 4320 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga 4380 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgccg 4440 ggatccagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 4500 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 4560 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 4620 ggtgtgggag gttttttagc ttggctgtgg aatgtgtgtc agttagggtg tggaaagtcc 4680 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 4740 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 4800 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 4860 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 4920 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 4980 aaaaagctcc tcgaggaact gaaaaaccag aaagttaact ggtaagttta gtctttttgt 5040 cttttatttc aggtcccgga tccggtggtg gtgcaaatca aagaactgct cctcagtgga 5100 tgttgccttt acttctaggc ctgtacggaa gtgttacttc tgctctaaaa gctgctgcag 5160 gagctcggac cgggccctta ggacgcgtaa tacgactcac tatagggaat tcgacgtcta 5220 gatcttaagg cgcgccaagg ggttggccac gtggtaacca cggggtggct agctagggat 5280 aacagggtaa tatagcggcc gccctttagt gagggttaat ttaaatcgta cgtcgcgatt 5340 aattaaccgc 5350 // ID AB004306; SV 2; linear; other DNA; STD; SYN; 2650 BP. XX AC AB004306; XX DT 31-OCT-1997 (Rel. 53, Created) DT 18-AUG-2007 (Rel. 92, Last updated, Version 7) XX DE Synthetic construct HPoc-Hosf-1 transgene for osteoblast stimulating DE factor-1, complete cds. XX KW osteoblast stimulating factor-1. XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-2650 RA Hashimoto-Gotoh T.; RT ; RL Submitted (27-MAY-1997) to the INSDC. RL Tamotsu Hashimoto-Gotoh, Kyoto Pref. Univ. of Medicine, RINDG, Dept. of RL Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kyoto 602, Japan RL (E-mail:thg@koto.kpu-m.ac.jp, Tel:075-251-5850, Fax:075-251-5799) XX RN [2] RX DOI; 10.1006/bbrc.1997.7188. RX PUBMED; 9299545. RA Masuda H., Tsujimura A., Yoshioka M., Arai Y., Kuboki Y., Mukai T., RA Nakamura T., Tsuji H., Nakagawa M., Hashimoto-Gotoh T.; RT "Bone mass loss due to estrogen deficiency is compensated in transgenic RT mice overexpressing human osteoblast stimulating factor-1"; RL Biochem. Biophys. Res. Commun. 238(2):528-533(1997). XX DR MD5; 33d49b5ee08944f56738b28442eaea57. XX FH Key Location/Qualifiers FH FT source 1..2650 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="human osteocalcin promoter-driven human osf-1 gene FT flanked by rabbit beta-globin 5'- and 3'- untranslated FT regions; HPoc-Hosf-1 transgene used to generate transgenic FT mice" FT /db_xref="taxon:32630" FT regulatory 15..882 FT /note="HPoc-Hosf-1 transgene" FT /note="derived from human osteocalcin promoter" FT /regulatory_class="promoter" FT regulatory 823..828 FT /regulatory_class="TATA_box" FT CDS 1542..2048 FT /codon_start=1 FT /transl_table=11 FT /product="osteoblast stimulating factor-1" FT /note="HPoc-Hosf-1 transgene" FT /note="derived from human osf-1 gene" FT /db_xref="GOA:P21246" FT /db_xref="HGNC:HGNC:9630" FT /db_xref="InterPro:IPR000762" FT /db_xref="InterPro:IPR020089" FT /db_xref="InterPro:IPR020090" FT /db_xref="InterPro:IPR020091" FT /db_xref="InterPro:IPR020092" FT /db_xref="InterPro:IPR037122" FT /db_xref="InterPro:IPR038130" FT /db_xref="PDB:2N6F" FT /db_xref="UniProtKB/Swiss-Prot:P21246" FT /protein_id="BAA22944.1" FT /translation="MQAQQYQQQRRKFAAAFLAFIFILAAVDTAEAGKKEKPEKKVKKS FT DCGEWQWSVCVPTSGDCGLGTREGTRTGAECKQTMKTQRCKIPCNWKKQFGAECKYQFQ FT AWGECDLNTALKTRTGSLKRALHNAECQKTVTISKPCGKLTKPKPQAESKKKKKEGKKQ FT EKMLD" XX SQ Sequence 2650 BP; 639 A; 657 C; 649 G; 705 T; 0 other; gaattcggca cgagctgcag ggtcaggagg agaatcgtgg ggccaggagg gcagaggcac 60 actccatctt cgtgctcctc acaggccctg cctccctgcc tgctaaggac acagggaagg 120 gggtccccac ctcagtgcct gcctcccttc cctgtgcctg tgtacctggc agtcacagcc 180 acctggcgtg tcccagaaac caaccggctg acctcatctc ctgcccggcc ccacctccat 240 tggctttggc ttttggcgtt tgtgctgccc gaccctttct cctgtccgga tgcgcagggc 300 agggcctgag ccgtcgagct gcacccacag caggctgcct ttggtgactc accgggtgaa 360 cgggggcatt gcgaggcatc ccctccctgg gtttggctcc tgcccacggg gctgacagta 420 gaaatcacag gctgtgagac agctggagcc cagctctgct tgaacctatt ttaggtctct 480 gatccccgct tcctctttag actcccctag agctcagcca gtgctcaacc tgaggctggg 540 ggtctctgag gaagagtgag ttggagctga ggggtctggg gctgtcccct gagagagggg 600 ccagaggcag tgtcaagagc cgggcagtct gattgtggct caccctccat cactcccagg 660 gcccctggcc cagcagccgc agctcccaac cacaatatcc tttggggttt ggcctacgga 720 gctggggcgg atgaccccca aatagccctg gcagattccc cctagacccg cccgcaccat 780 ggtcaggcat gcccctcctc atcgctggca cagcccagag ggtataaaca gtgctggagg 840 ctggcggggc aggccagctg agtcctgagc agcagcccag cggatcctga gaacttcagg 900 gtgagtttgg ggacccttga ttgttctttc tttttcgcta ttgtaaaatt catgttatat 960 ggagggggca aagttttcag ggtgttgttt agaatgggaa gatgtccctt gtatcaccat 1020 ggaccctcat gataattttg tttctttcac tttctactct gttgacaacc attgtctcct 1080 cttattttct tttcattttc tgtaactttt tcgttaaact ttagcttgca tttgtaacga 1140 atttttaaat tcacttttgt ttatttgtca gattgtaagt actttctcta atcacttttt 1200 tttcaaggca atcagggtat attatattgt acttcagcac agttttagag aacaattgtt 1260 ataattaaat gataaggtag aatatttctg catataaatt ctggctggcg tggaaatatt 1320 cttattggta gaaacaacta catcctggtc atcatcctgc ctttctcttt atggttacaa 1380 tgatatacac tgtttgagat gaggataaaa tactctgagt ccaaaccggg cccctctgct 1440 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttgttgtg 1500 ctgtctcatc attttggcaa agaattaatt ccaactcaaa aatgcaggct caacagtacc 1560 agcagcagcg tcgaaaattt gcagctgcct tcttggcatt cattttcata ctggcagctg 1620 tggatactgc tgaagcaggg aagaaagaga aaccagaaaa aaaagtgaag aagtctgact 1680 gtggagaatg gcagtggagt gtgtgtgtgc ccaccagtgg agactgtggg ctgggcacac 1740 gggagggcac tcggactgga gctgagtgca agcaaaccat gaagacccag agatgtaaga 1800 tcccctgcaa ctggaagaag caatttggcg cggagtgcaa ataccagttc caggcctggg 1860 gagaatgtga cctgaacaca gccctgaaga ccagaactgg aagtctgaag cgagccctgc 1920 acaatgccga atgccagaag actgtcacca tctccaagcc ctgtggcaaa ctgaccaagc 1980 ccaaacctca agcagaatct aagaagaaga aaaaggaagg caagaaacag gagaagatgc 2040 tggattaaaa gatgtcacct gtggaacata aaaaggacat cagcaaacag gatcaattca 2100 ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat gccctggctc 2160 acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc 2220 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga 2280 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga 2340 atgagtattt ggtttagagt ttggcaacat atgccatatg ctggctgcca tgaacaaagg 2400 tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc cttattccat 2460 agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt atttttttct 2520 ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct cctctcctga 2580 ctactcccag tcatagctgt ccctcttctc ttatggagat ccctcgacct gcaggcaggc 2640 atgcaagctt 2650 // ID AB005475; SV 2; linear; other DNA; STD; SYN; 4211 BP. XX AC AB005475; XX DT 23-JUL-1997 (Rel. 52, Created) DT 20-JUN-2005 (Rel. 84, Last updated, Version 4) XX DE Cloning vector pMW118 gene for repA protein, beta-lactamase, complete and DE partial cds. XX KW . XX OS Cloning vector pMW118 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-4211 RA Watahiki M., Hayashi H.; RT ; RL Submitted (02-JUL-1997) to the INSDC. RL Masanori Watahiki, Nippon Gene Co., Ltd., Research & Development; RL Tonya-machi 1-29, Toyama, Toyama, 930-0834, Japan RL (E-mail:masanori.watahiki@pref.toyama.lg.jp, Tel:81-764-51-6548, RL Fax:81-764-51-6547) XX RN [2] RA Watahiki M.; RT "pMW118, low-copy number plasmid vector in Escherichia coli"; RL Unpublished. XX DR MD5; c2c04c82251394edfd9bec305c310285. DR EuropePMC; PMC2532685; 18699991. DR EuropePMC; PMC3251776; 22159605. DR EuropePMC; PMC6449708; 30993221. DR EuropePMC; PMC95182; 11274125. XX FH Key Location/Qualifiers FH FT source 1..4211 FT /organism="Cloning vector pMW118" FT /lab_host="Escherichia coli" FT /mol_type="other DNA" FT /db_xref="taxon:62527" FT misc_binding 482..492 FT /bound_moiety="dnaA" FT misc_binding 547..558 FT /bound_moiety="Escherichia coli IHF" FT protein_bind 577..601 FT /bound_moiety="RepA protein" FT CDS 750..1700 FT /transl_table=11 FT /product="repA protein" FT /protein_id="BAA21463.2" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI" FT misc_feature 2284..2340 FT /note="multi-cloning site" FT CDS complement(<3144..4001) FT /transl_table=11 FT /product="beta-lactamase" FT /protein_id="BAA21464.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" XX SQ Sequence 4211 BP; 1278 A; 913 C; 944 G; 1076 T; 0 other; gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactgggtgc attagccagt 60 ctgaatgacc tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa 120 ctgcagaaca gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga 180 gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag 240 gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat 300 gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg 360 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta 420 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg 480 agttatacac agggctggga tctattcttt ttatcttttt ttattctttc tttattctat 540 aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag 600 agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta cagataccca 660 caactcaaag gaaaaggact agtaattatc attgactagc ccatctcaat tggtatagtg 720 attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg 780 aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg 840 tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca 900 cttataacca atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag 960 ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag 1020 gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag 1080 tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc 1140 tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa 1200 aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta 1260 agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt 1320 tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc 1380 gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg 1440 taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta 1500 catcagattc ctacctacat aacggactaa gaaaaacact acacgatgct ttaactgcaa 1560 aaattcagct caccagtttt gaggcaaaat ttttgagtga catgcaaagt aagcatgatc 1620 tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg 1680 ctaaatacgg aaggatctga ggttcttatg gctcttgtat ctatcagtga agcatcaaga 1740 ctaacaaaca aaagtagaac aactgttcac cgttacatat caaagggaaa actgtccata 1800 tgcacagatg aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt 1860 gcattcaaag ctgttcacca tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca 1920 cctaatagaa caggtgaaac cagtaaaaca aagcaactag aacatgaaat tgaacacctg 1980 agacaacttg ttacagctca acagtcacac atagacagcc tgaaacaggc gatgctgctt 2040 atcgaatcaa agctgccgac aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca 2100 tggcaattct ggaagaaata gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 2160 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 2220 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 2280 gccaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc 2340 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 2400 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 2460 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 2520 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct ttctcaatgc 2580 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 2640 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 2700 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 2760 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 2820 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 2880 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 2940 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 3000 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 3060 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 3120 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 3180 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 3240 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 3300 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 3360 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 3420 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg 3480 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 3540 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 3600 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 3660 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 3720 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat 3780 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 3840 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 3900 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 3960 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 4020 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 4080 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 4140 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 4200 cttcaagaat t 4211 // ID AB005476; SV 2; linear; other DNA; STD; SYN; 4211 BP. XX AC AB005476; XX DT 23-JUL-1997 (Rel. 52, Created) DT 20-JUN-2005 (Rel. 84, Last updated, Version 3) XX DE Cloning vector pMW119 DNA for repA protein and beta-lactamase, complete and DE partial cds. XX KW . XX OS Cloning vector pMW119 OC other sequences; artificial sequences; vectors. OG Plasmid pMW119 XX RN [1] RP 1-4211 RA Watahiki M., Hayashi H.; RT ; RL Submitted (02-JUL-1997) to the INSDC. RL Masanori Watahiki, Nippon Gene Co., Ltd., Research & Development; RL Tonya-machi 1-29, Toyama, Toyama, 930-0834, Japan RL (E-mail:masanori.watahiki@pref.toyama.lg.jp, Tel:81-764-51-6548, RL Fax:81-764-51-6547) XX RN [2] RA Watahiki M.; RT "pMW119, low-copy number plasmid vector in Escherichia coli"; RL Unpublished. XX DR MD5; e1260b64b8fd20825a1d7706771cdbd4. DR EuropePMC; PMC111357; 10940021. DR EuropePMC; PMC95182; 11274125. XX FH Key Location/Qualifiers FH FT source 1..4211 FT /organism="Cloning vector pMW119" FT /plasmid="pMW119" FT /lab_host="Escherichia coli" FT /mol_type="other DNA" FT /db_xref="taxon:62528" FT misc_binding 482..492 FT /bound_moiety="dnaA" FT misc_binding 547..558 FT /bound_moiety="Escherichia coli IHF" FT protein_bind 577..601 FT /bound_moiety="RepA protein" FT CDS 750..1700 FT /transl_table=11 FT /product="repA protein" FT /protein_id="BAA21465.2" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI" FT misc_feature 2281..2337 FT /note="multi-cloning site" FT CDS complement(<3144..4001) FT /transl_table=11 FT /product="beta-lactamase" FT /protein_id="BAA21466.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" XX SQ Sequence 4211 BP; 1278 A; 911 C; 946 G; 1076 T; 0 other; gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactgggtgc attagccagt 60 ctgaatgacc tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa 120 ctgcagaaca gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga 180 gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag 240 gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat 300 gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg 360 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta 420 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg 480 agttatacac agggctggga tctattcttt ttatcttttt ttattctttc tttattctat 540 aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag 600 agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta cagataccca 660 caactcaaag gaaaaggact agtaattatc attgactagc ccatctcaat tggtatagtg 720 attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg 780 aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg 840 tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca 900 cttataacca atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag 960 ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag 1020 gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag 1080 tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc 1140 tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa 1200 aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta 1260 agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt 1320 tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc 1380 gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg 1440 taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta 1500 catcagattc ctacctacat aacggactaa gaaaaacact acacgatgct ttaactgcaa 1560 aaattcagct caccagtttt gaggcaaaat ttttgagtga catgcaaagt aagcatgatc 1620 tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg 1680 ctaaatacgg aaggatctga ggttcttatg gctcttgtat ctatcagtga agcatcaaga 1740 ctaacaaaca aaagtagaac aactgttcac cgttacatat caaagggaaa actgtccata 1800 tgcacagatg aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt 1860 gcattcaaag ctgttcacca tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca 1920 cctaatagaa caggtgaaac cagtaaaaca aagcaactag aacatgaaat tgaacacctg 1980 agacaacttg ttacagctca acagtcacac atagacagcc tgaaacaggc gatgctgctt 2040 atcgaatcaa agctgccgac aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca 2100 tggcaattct ggaagaaata gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 2160 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 2220 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 2280 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc 2340 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 2400 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 2460 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 2520 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct ttctcaatgc 2580 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 2640 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 2700 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 2760 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 2820 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 2880 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 2940 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 3000 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 3060 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 3120 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 3180 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 3240 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 3300 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 3360 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 3420 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg 3480 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 3540 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 3600 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 3660 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 3720 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat 3780 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 3840 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 3900 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 3960 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 4020 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 4080 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 4140 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 4200 cttcaagaat t 4211 // ID AB005477; SV 2; linear; other DNA; STD; SYN; 3923 BP. XX AC AB005477; XX DT 23-JUL-1997 (Rel. 52, Created) DT 20-JUN-2005 (Rel. 84, Last updated, Version 3) XX DE Cloning vector pMW218 DNA for repA protein and neomycin-kanamycin DE phosphotransferase, complete cds. XX KW . XX OS Cloning vector pMW218 OC other sequences; artificial sequences; vectors. OG Plasmid pMW218 XX RN [1] RP 1-3923 RA Watahiki M., Hayashi H.; RT ; RL Submitted (02-JUL-1997) to the INSDC. RL Masanori Watahiki, Nippon Gene Co., Ltd., Research & Development; RL Tonya-machi 1-29, Toyama, Toyama, 930-0834, Japan RL (E-mail:masanori.watahiki@pref.toyama.lg.jp, Tel:81-764-51-6548, RL Fax:81-764-51-6547) XX RN [2] RA Watahiki M.; RT "pMW218, low-copy number plasmid vector in Escherichia coli"; RL Unpublished. XX DR MD5; 7cbda01654abefdd8008ad4d4a93fa5e. DR EuropePMC; PMC3807490; 23974030. XX FH Key Location/Qualifiers FH FT source 1..3923 FT /organism="Cloning vector pMW218" FT /plasmid="pMW218" FT /lab_host="Escherichia coli" FT /mol_type="other DNA" FT /db_xref="taxon:62529" FT misc_binding 482..492 FT /bound_moiety="dnaA" FT misc_binding 547..558 FT /bound_moiety="Escherichia coli IHF" FT protein_bind 577..601 FT /bound_moiety="RepA protein" FT CDS 750..1700 FT /transl_table=11 FT /product="repA protein" FT /protein_id="BAA21467.2" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI" FT misc_feature 2284..2340 FT /note="multi-cloning site" FT CDS complement(2657..3451) FT /transl_table=11 FT /product="neomycin-kanamycin phosphotransferase" FT /protein_id="BAA21468.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVGTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" XX SQ Sequence 3923 BP; 1181 A; 904 C; 906 G; 932 T; 0 other; gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactgggtgc attagccagt 60 ctgaatgacc tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa 120 ctgcagaaca gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga 180 gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag 240 gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat 300 gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg 360 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta 420 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg 480 agttatacac agggctggga tctattcttt ttatcttttt ttattctttc tttattctat 540 aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag 600 agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta cagataccca 660 caactcaaag gaaaaggact agtaattatc attgactagc ccatctcaat tggtatagtg 720 attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg 780 aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg 840 tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca 900 cttataacca atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag 960 ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag 1020 gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag 1080 tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc 1140 tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa 1200 aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta 1260 agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt 1320 tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc 1380 gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg 1440 taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta 1500 catcagattc ctacctacat aacggactaa gaaaaacact acacgatgct ttaactgcaa 1560 aaattcagct caccagtttt gaggcaaaat ttttgagtga catgcaaagt aagcatgatc 1620 tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg 1680 ctaaatacgg aaggatctga ggttcttatg gctcttgtat ctatcagtga agcatcaaga 1740 ctaacaaaca aaagtagaac aactgttcac cgttacatat caaagggaaa actgtccata 1800 tgcacagatg aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt 1860 gcattcaaag ctgttcacca tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca 1920 cctaatagaa caggtgaaac cagtaaaaca aagcaactag aacatgaaat tgaacacctg 1980 agacaacttg ttacagctca acagtcacac atagacagcc tgaaacaggc gatgctgctt 2040 atcgaatcaa agctgccgac aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca 2100 tggcaattct ggaagaaata gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 2160 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 2220 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 2280 gccaagcttg catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc 2340 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 2400 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 2460 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 2520 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct ttctcatagc 2580 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgccg 2640 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg 2700 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct 2760 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc 2820 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg 2880 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga 2940 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac 3000 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc 3060 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct 3120 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc 3180 agtcccttcc cgcttcagtg ccaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg 3240 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg 3300 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc 3360 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag 3420 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat 3480 cactaccgca ttaaagcata tcgatgataa gctgtcaaac atgagcgttc ttcggggcga 3540 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 3600 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 3660 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 3720 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 3780 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 3840 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 3900 aggccctttc gtcttcaaga att 3923 // ID AB005478; SV 2; linear; other DNA; STD; SYN; 3923 BP. XX AC AB005478; XX DT 23-JUL-1997 (Rel. 52, Created) DT 20-JUN-2005 (Rel. 84, Last updated, Version 3) XX DE Cloning vector pMW219 DNA for repA protein and neomycin-kanamycin DE phosphotransferase, complete cds. XX KW . XX OS Cloning vector pMW219 OC other sequences; artificial sequences; vectors. OG Plasmid pMW219 XX RN [1] RP 1-3923 RA Watahiki M., Hayashi H.; RT ; RL Submitted (02-JUL-1997) to the INSDC. RL Masanori Watahiki, Nippon Gene Co., Ltd., Research & Development; RL Tonya-machi 1-29, Toyama, Toyama, 930-0834, Japan RL (E-mail:masanori.watahiki@pref.toyama.lg.jp, Tel:81-764-51-6548, RL Fax:81-764-51-6547) XX RN [2] RA Watahiki M.; RT "pMW219, low-copy number plasmid vector in Escherichia coli"; RL Unpublished. XX DR MD5; e4f261055143af9f3289e7626c73ec56. DR EuropePMC; PMC95182; 11274125. XX FH Key Location/Qualifiers FH FT source 1..3923 FT /organism="Cloning vector pMW219" FT /plasmid="pMW219" FT /lab_host="Escherichia coli" FT /mol_type="other DNA" FT /db_xref="taxon:62530" FT misc_binding 482..492 FT /bound_moiety="dnaA" FT misc_binding 547..558 FT /bound_moiety="Escherichia coli IHF" FT protein_bind 577..601 FT /bound_moiety="RepA protein" FT CDS 750..1700 FT /transl_table=11 FT /product="repA protein" FT /protein_id="BAA21469.2" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI" FT misc_feature 2281..2337 FT /note="multi-cloning site" FT CDS complement(2657..3451) FT /transl_table=11 FT /product="neomycin-kanamycin phosphotransferase" FT /protein_id="BAA21470.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVGTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" XX SQ Sequence 3923 BP; 1181 A; 902 C; 908 G; 932 T; 0 other; gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactgggtgc attagccagt 60 ctgaatgacc tgtcacggga taatccgaag tggtcagact ggaaaatcag agggcaggaa 120 ctgcagaaca gcaaaaagtc agatagcacc acatagcaga cccgccataa aacgccctga 180 gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa tccataaaag 240 gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag cgaactgaat 300 gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga atattcagcg 360 atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt ctgttttgta 420 gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac taaagtagtg 480 agttatacac agggctggga tctattcttt ttatcttttt ttattctttc tttattctat 540 aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg gaatttacag 600 agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta cagataccca 660 caactcaaag gaaaaggact agtaattatc attgactagc ccatctcaat tggtatagtg 720 attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc aaagcaaatg 780 aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt ttatgctgtg 840 tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg gtatcgttca 900 cttataacca atacgctcag atgatgaaca tcagtaggga aaatgcttat ggtgtattag 960 ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct ttggttaaag 1020 gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa ttagaattag 1080 tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata aaatataatc 1140 tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag tggttattaa 1200 aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt gatgaattta 1260 agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac caatgggttt 1320 tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg gttgataagc 1380 gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa atggatctcg 1440 taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca acaaccatta 1500 catcagattc ctacctacat aacggactaa gaaaaacact acacgatgct ttaactgcaa 1560 aaattcagct caccagtttt gaggcaaaat ttttgagtga catgcaaagt aagcatgatc 1620 tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag aacatactgg 1680 ctaaatacgg aaggatctga ggttcttatg gctcttgtat ctatcagtga agcatcaaga 1740 ctaacaaaca aaagtagaac aactgttcac cgttacatat caaagggaaa actgtccata 1800 tgcacagatg aaaacggtgt aaaaaagata gatacatcag agcttttacg agtttttggt 1860 gcattcaaag ctgttcacca tgaacagatc gacaatgtaa cagatgaaca gcatgtaaca 1920 cctaatagaa caggtgaaac cagtaaaaca aagcaactag aacatgaaat tgaacacctg 1980 agacaacttg ttacagctca acagtcacac atagacagcc tgaaacaggc gatgctgctt 2040 atcgaatcaa agctgccgac aacacgggag ccagtgacgc ctcccgtggg gaaaaaatca 2100 tggcaattct ggaagaaata gcgccattcg ccattcaggc tgcgcaactg ttgggaaggg 2160 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg 2220 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt 2280 gaattcgagc tcggtacccg gggatcctct agagtcgacc tgcaggcatg caagcttggc 2340 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 2400 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 2460 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 2520 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct ttctcatagc 2580 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgccg 2640 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg 2700 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct 2760 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc 2820 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg 2880 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga 2940 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac 3000 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc 3060 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct 3120 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc 3180 agtcccttcc cgcttcagtg ccaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg 3240 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg 3300 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc 3360 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag 3420 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat 3480 cactaccgca ttaaagcata tcgatgataa gctgtcaaac atgagcgttc ttcggggcga 3540 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 3600 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 3660 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 3720 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 3780 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 3840 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 3900 aggccctttc gtcttcaaga att 3923 // ID AB007632; SV 1; circular; other DNA; STD; SYN; 4234 BP. XX AC AB007632; XX DT 14-OCT-1997 (Rel. 52, Created) DT 10-MAR-2009 (Rel. 100, Last updated, Version 5) XX DE Unidentified cloning vector pSSD3, complete sequence. XX KW . XX OS unidentified cloning vector OC other sequences; artificial sequences; vectors. OG Plasmid pSSD3 XX RN [1] RP 1-4234 RA Kato S., Saeki M.; RT ; RL Submitted (26-SEP-1997) to the INSDC. RL Contact:Seishi Kato Research Institute of National Rehabilitation Center RL for the Disabled, Department of Rehabilitation Engineering; 4-1 Namiki, RL Tokorozawa, Saitama 359-8555, Japan XX RN [2] RP 1-4234 RA Kato S., Saeki M.; RT "Signal sequence detection vector pSSD3"; RL Unpublished. XX DR MD5; 90c5dfd6f799b2bbf81e341a04e49cd8. XX FH Key Location/Qualifiers FH FT source 1..4234 FT /organism="unidentified cloning vector" FT /plasmid="pSSD3" FT /mol_type="other DNA" FT /note="Signal sequence detection vector pSSD3" FT /db_xref="taxon:45196" FT misc_feature 30..240 FT /note="SV40 early promoter" FT misc_feature 279 FT /note="SV40 origin" FT misc_feature 549..565 FT /note="T7 RNA polymerase promoter" FT misc_feature 581..586 FT /note="SmaI site for cloning of a target cDNA fragment FT encoding a putative signal sequence" FT misc_feature 588..593 FT /note="PmaCI site for cloning of a target cDNA fragment FT encoding a putative signal sequence" FT misc_feature 595..600 FT /note="EcoRV site for cloning of a target cDNA fragment FT encoding a putative signal sequence" FT misc_feature 600..1427 FT /note="cDNA fragment encoding human urokinase C-terminal FT protease domain" FT misc_feature 2701..3561 FT /note="beta-lactamase gene" FT misc_feature 3693..4148 FT /note="f1 phage IG region" XX SQ Sequence 4234 BP; 1088 A; 1072 C; 1048 G; 1026 T; 0 other; agcttggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 60 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 120 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 180 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 240 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt 300 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcctcgagga 360 actgaaaaac cagaaagtta actggtaagt ttagtctttt tgtcttttat ttcaggtccc 420 ggatccggtg gtggtgcaaa tcaaagaact gctcctcagt ggatgttgcc tttacttcta 480 ggcctgtacg gaagtgttac ttctgctcta aaagctgctc gagtgtaaaa cgacggccag 540 tacgtattta atacgactca ctatagggaa ttccacagat cccgggtcac gtgggatatc 600 cctcctctcc tccagaagaa ttaaaatttc agtgtggcca aaagactctg aggccccgct 660 ttaagattat tgggggagaa ttcaccacca tcgagaacca gccctggttt gcggccatct 720 acaggaggca ccgggggggc tctgtcacct acgtgtgtgg aggcagcctc atcagccctt 780 gctgggtgat cagcgccaca cactgcttca ttgattaccc aaagaaggag gactacatcg 840 tctacctggg tcgctcaagg cttaactcca acacgcaagg ggagatgaag tttgaggtgg 900 aaaacctcat cctacacaag gactacagcg ctgacacgct tgctcaccac aatgacattg 960 ccttgctgaa gatccgttcc aaggagggca ggtgtgcgca gccatcccgg actatacaga 1020 ccatctgcct gccctcgatg tataacgatc cccagtttgg cacaagctgt gagatcactg 1080 gctttggaaa agagaattct accgactatc tctatccgga gcagctgaaa atgactgttg 1140 tgaagctgat ttcccaccgg gagtgtcagc agccccacta ctacggctct gaagtcacca 1200 ccaaaatgct gtgtgctgct gacccacagt ggaaaacaga ttcctgccag ggagactcag 1260 ggggacccct cgtctgttcc ctccaaggcc gcatgacttt gactggaatt gtgagctggg 1320 gccgtggatg tgccctgaag gacaagccag gcgtctacac gagagtctca cacttcttac 1380 cctggatccg cagtcacacc aaggaagaga atggcctggc cctctgaggg tccccaggga 1440 ggaaacgggc accacccgct ttcttgctgg taccgcggcc gcggatctcc ctttagtgag 1500 ggttaattgg atccagacat gataagatac attgatgagt ttggacaaac cacaactaga 1560 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc 1620 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt 1680 cagggggagg tgtgggaggt tttttctgca ttaatgaatc ggccaacgcg cggggagagg 1740 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 1800 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 1860 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 1920 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 1980 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 2040 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 2100 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 2160 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 2220 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 2280 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 2340 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 2400 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 2460 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 2520 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 2580 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 2640 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 2700 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 2760 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 2820 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 2880 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 2940 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 3000 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 3060 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 3120 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 3180 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 3240 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 3300 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 3360 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 3420 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 3480 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 3540 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 3600 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 3660 tccgcgcaca tttccccgaa aagtgccacc tgaaattgta aacgttaata ttttgttaaa 3720 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa 3780 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa 3840 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca 3900 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg 3960 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc 4020 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc 4080 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca 4140 gggcgcgtcc cattcgccat tcacacagga aacagctatg accatgatcc tctagagtcg 4200 acctgcaggc atgcaagcta ggcctacatg gcca 4234 // ID AB009864; SV 2; circular; other DNA; STD; SYN; 3392 BP. XX AC AB009864; XX DT 05-JAN-1998 (Rel. 54, Created) DT 10-MAY-2005 (Rel. 83, Last updated, Version 3) XX DE Expression vector pME18S-FL3-3 DNA, complete sequence. XX KW . XX OS Expression vector pME18S-FL3-3 OC other sequences; artificial sequences; vectors. OG Plasmid pME18S-FL3-3 XX RN [1] RP 1-3392 RA Maruyama K., Sugano S.; RT ; RL Submitted (16-DEC-1997) to the INSDC. RL Sumio Sugano, The Institute of Medical Science, University of Tokyo, RL Department of Virology; 4-6-1, Shirokanedai, Minatoku, Tokyo, 108, Japan RL (E-mail:ssugano@ims.u-tokyo.ac.jp, Tel:81-3-5449-5286, Fax:81-3-5449-5416) XX RN [2] RA Maruyama K., Sugano S.; RT "pME18S-FL3-3: a versatile expression vector"; RL Unpublished. XX DR MD5; eefce6beb9eee8293fcbafb771584b9c. DR EuropePMC; PMC117397; 11782535. DR EuropePMC; PMC2195720; 10601355. DR EuropePMC; PMC2287170; 18294402. DR EuropePMC; PMC2866332; 20479877. DR EuropePMC; PMC55463; 11353085. DR EuropePMC; PMC6520346; 31092829. DR EuropePMC; PMC6650486; 31337761. XX FH Key Location/Qualifiers FH FT source 1..3392 FT /organism="Expression vector pME18S-FL3-3" FT /plasmid="pME18S-FL3-3" FT /mol_type="other DNA" FT /db_xref="taxon:327037" FT regulatory 1..638 FT /regulatory_class="promoter" FT polyA_site 1229..1427 XX SQ Sequence 3392 BP; 811 A; 900 C; 818 G; 863 T; 0 other; aagcttggct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag 60 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag 120 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc 180 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc 240 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat 300 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcctccgat 360 cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta cctgaggccg ccatccacgc 420 cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct cctgaactgc gtccgccgtc 480 taggtaagtt taaagctcag gtcgagaccg ggcctttgtc cggcgctccc ttggagccta 540 cctagactca gccggctctc cacgctttgc ctgaccctgc ttgctcaact ctacgtcttt 600 gtttcgtttt ctgttctgcg ccgttacaga tccaagctct gaaaaaccag aaagttaact 660 ggtaagttta gtctttttgt cttttatttc aggtcccgga tccggtggtg caaatcaaag 720 aactgctcct cagtggatgt tgcctttact tctaggcctg tacggaagtg ttacttctgc 780 tctaaaagct gcggaattcc tcgagcactg tgtgatatcc attgtgctgg cgcggattct 840 ttatcactga taagttggtg gacatattat gtttatcagt gataaagtgt caagcatgac 900 aaagttgcag ccgaatacag tgatccgtgc cgccctagac ctgttgaacg aggtcggcgt 960 agacggtctg acgacacgca aactggcgga acggttgggg gttcagcagc cggcgcttta 1020 ctggcacttc aggaacaagc gggcgctgct cgacgcactg gccgaagcca tgctggcgga 1080 gaatcatagc acttcggtgc cgagagccga cgacgactgg cgctcatttc tgactgggaa 1140 tgcccgcagc ttcaggcagg cgctgctcgc ctaccgccag cacaatggca ccatgtgctc 1200 gagctgcagg tcgcggccgc tagactagtc tagagaaaaa acctcccaca cctccccctg 1260 aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat 1320 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 1380 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccccgggtac 1440 cgagctcgaa ttaattcctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 1500 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 1560 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 1620 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 1680 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 1740 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 1800 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 1860 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 1920 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 1980 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 2040 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 2100 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 2160 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 2220 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 2280 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 2340 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 2400 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 2460 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 2520 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 2580 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 2640 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 2700 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 2760 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 2820 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 2880 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 2940 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 3000 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 3060 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 3120 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 3180 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 3240 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 3300 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 3360 ccgcgcacat ttccccgaaa agtgccacct gc 3392 // ID AB009992; SV 1; linear; other DNA; STD; SYN; 591 BP. XX AC AB009992; XX DT 22-JAN-2003 (Rel. 74, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct mwti1b gene, complete cds, encording trypsin inhibitor DE of winged bean. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-591 RA Mochizuki A.; RT ; RL Submitted (20-DEC-1997) to the INSDC. RL Atsushi Mochizuki, Tohoku National Agricultural Experiment Station, RL Department of Lowland Farming; Shimofurumichi 3, Yotsuya, Omagari, Akita RL 014-01, Japan (E-mail:lepi@affrc.go.jp, Tel:+81-187-66-1221, RL Fax:+81-187-66-2639) XX RN [2] RA Mochizuki A.; RT "Artificial sequence encoding winged bean trypsin inhibitor, WTI-1B"; RL Unpublished. XX DR MD5; b09534e2ecfb0808b9065ca9e6cbffb5. XX FH Key Location/Qualifiers FH FT source 1..591 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..591 FT /codon_start=1 FT /transl_table=11 FT /gene="mwti1b" FT /note="This gene conferres resistance to chilo suppresalis, FT a pest insect of rice, on the transgenic rice." FT /note="encording trypsin inhibitor of winged bean" FT /protein_id="BAC55863.1" FT /translation="MKSTTFLALFLLSAIISHLPSSTAEPLLDSEGELVRNGGTYYLLP FT DRWALGGGIEAAATGTETCPLTVVRSPNEVSVGEPLRISSQLRSGFIPDYSLVRIGFAN FT PPKCAPSPWWTVVEDQPQQPSVKLSELKSTKFDYLFKFEKVTSKFSSYKLKYCAKRDTC FT KDIGIYRDQKGYARLVVTDENPLVVIFKKVESS" XX SQ Sequence 591 BP; 165 A; 138 C; 129 G; 159 T; 0 other; atgaagagta ctacatttct tgccctcttt ctactctctg ccatcatctc acacctacca 60 tcatccactg ctgagccatt gctcgattct gaaggtgagt tagttcgaaa tggtggcaca 120 tactatctgt tgccagatag atgggcactc gggggaggaa tagaagcagc agcaacagga 180 accgaaacat gccctctaac agtggtacga tctcccaatg aggtctctgt aggggaacca 240 ttaaggatct catcccaatt gcgttcaggg ttcatccccg attactctct agtgcgtatt 300 ggattcgcta accctccaaa gtgtgcacct tctccgtggt ggactgttgt tgaggaccaa 360 ccacaacaac cctctgttaa acttagtgag ctaaaatcta ctaaattcga ttatctattc 420 aaattcgaga aagttacctc taagttttcc tcatacaagc ttaagtactg tgccaagagg 480 gacacctgta aggatatcgg gatctatagg gatcagaaag gatacgcacg tttggtggtg 540 actgacgaaa acccattagt ggttatcttt aaaaaggtgg agtcaagcta a 591 // ID AB012282; SV 1; linear; other DNA; STD; SYN; 6687 BP. XX AC AB012282; XX DT 30-MAR-1998 (Rel. 55, Created) DT 18-AUG-2007 (Rel. 92, Last updated, Version 4) XX DE Shuttle vector pAUR101 gene for Aur1-C, complete cds. XX KW Aur1-C variant; aureobasidin resiatant protein. XX OS Shuttle vector pAUR101 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-6687 RA Hashida-Okado T.; RT ; RL Submitted (18-MAR-1998) to the INSDC. RL Takashi Hashida-Okado, Takara Shuzo Co. ,Ltd., Biotechnology Research RL Laboratories; Seta 3-4-1, Otsu, Shiga 520-2193, Japan RL (E-mail:okadot@takara.co.jp, Tel:81-77-543-7298, Fax:81-77-543-2494) XX RN [2] RA Hashida-Okado T.; RT "Yeast-E. coli shuttle vector pAUR101"; RL Unpublished. XX DR MD5; e4c946e54ce33240d55bd9f5df48881c. DR EuropePMC; PMC3338270; 22367032. XX CC This vector can be obtained from Takara Shuzo Co. Ltd., CC Biotechnology Research Lab., Seta 3-4-1, Otsu, Shiga, 520-2193, CC Japan. To place an order FAX +81-77-565-6978. For technical CC information, FAX +81-77-565-6965. This sequence has been compiled CC from information in the sequence obtained by Takara Shuzo's CC Technical Service Department at FAX +81-77-565-6965. XX FH Key Location/Qualifiers FH FT source 1..6687 FT /organism="Shuttle vector pAUR101" FT /mol_type="other DNA" FT /db_xref="taxon:75305" FT CDS 3805..5010 FT /codon_start=1 FT /transl_table=11 FT /gene="AUR1-C" FT /product="Aur1-C" FT /function="aureobasidin resistant" FT /note="variant" FT /protein_id="BAA25319.1" FT /translation="MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSDW FT VHYIFLGSIMLFVFITNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALYFT FT SSYFPDDRRPPITVKVLPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHYGAPFV FT VAAILFVFGPPTVLQGYAFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDMHGSP FT GGLARIDKLLGINMYTTCFSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPLFIAYV FT CWLWWSTMYLTHHYFVDLMAGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSIEKYDIS FT KSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATSITSLGVK FT RA" XX SQ Sequence 6687 BP; 1669 A; 1541 C; 1401 G; 2076 T; 0 other; gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 60 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 120 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 180 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 240 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 300 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 360 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 420 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 480 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 540 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 600 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 660 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 720 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 780 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 840 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 900 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 960 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 1020 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1080 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1140 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1200 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1260 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1320 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 1380 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1440 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1500 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1560 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1620 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1680 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1740 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1800 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1860 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1920 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1980 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 2040 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 2100 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc 2160 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg 2220 accatgatta cgccaagctt tgtaaagttc ttcttccaga ttgtgcgcaa actcttgcga 2280 tatcgatata acgtctttcc catcaggaag ctcataaagt tttgcctcaa ttgtttcggg 2340 aacgatgaat ttgctaaata aggtagagaa cattttttct gcattatacc tcagtttgtg 2400 ttctttttcg ctttcaaaat ctttcctagt aggcatttcg ttttcggcag tatcagactt 2460 ggcggcatca atgcttttat tccttttttt tgacccattg gatttcttca cctgtttgga 2520 tttgatatga attcctttgg cactatcggg gctccttttt cgtttcctag ggctttcctc 2580 atcaagaaag acatctgcat cattgtcatc atggtcattt acgggcttgt aaacatcatc 2640 atggtaatct tcatcctctg aaacctctgc ctcctttgac gtttccaaat gtgcatacaa 2700 tgatgggtcg cacaattcac agtagtactt agaatcttca ctcattagcc catctatggt 2760 gtctttacca tcagtcatac atttaatatg ctgccaagtg tcacagccat cgcactgtac 2820 catgtcgcca tgcgaatatt ctgcagcatc gtagttttca ttgttcgccc cacacagaca 2880 tctaacatag ccctcatccg cttcgtctac ttcgtctgtt cttacatttt cagtatcttt 2940 ccttggctcc tgagaagaat cagacttctt attcttctct atggctgaat caactttctt 3000 tttagttctt tttttctttg gtgcttcagt ctcctcctgt aacaaatact caatatattt 3060 attctgaccc ttattagatc ttgaagaagt acggacagac ataataaatt taaactaaat 3120 aaaagctaaa agtaaaagag aacctaggat aatcgatagc tccctgtgta tagaacaagt 3180 caaaatacta agatctcgtc aacctatcct ctttcattgc aagccttttg gccaaactgc 3240 agtctgggca tccctttgca tgtcttgtga aatagtatcc atctctcccc agctcccaga 3300 aatgatatga aaagttattc gtgaagagtg atcctaaaac tgaaaaaagg aaaaaacaaa 3360 aatgagctaa aaaaaccaca acaaaagaat atcaacttca ttgcttggcg ggtcatcgct 3420 tttttatttc tttctgtcaa agaataataa agtgcccatc agtgttcata tttgttacaa 3480 agtggttttc tgatttggta ctactgcaga ggcgtatttt ttgcttcagt taccatagcg 3540 taagaacact agcgactttt gttcgtgaac caacagagta ggatttctac tgctacatct 3600 cttaggtagt tggttagtcc gatcgctcac ttttggttgt tgttaagtac ttcataagtt 3660 tatccttttc ctttttcaca ctgagctact ttgggtatag cttttggccc aaggatcttt 3720 gaattttctc caaaagtact ttattttata tcctacaggt tgcggttttc atattttaaa 3780 aagcttttaa tcattccttt gcgtatggca aacccttttt cgagatggtt tctatcagag 3840 agacctccaa actgccatgt agccgattta gaaacaagtt tagatcccca tcaaacgttg 3900 ttgaaggtgc aaaaatacaa acccgcttta agcgactggg tgcattacat cttcttggga 3960 tccatcatgc tgtttgtgtt cattactaat cccgcacctt ggatcttcaa gatccttttt 4020 tattgtttct tgggcacttt attcatcatt ccagctacgt cacagttttt cttcaatgcc 4080 ttgcccatcc taacatgggt ggcgctgtat ttcacttcat cgtactttcc agatgaccgc 4140 aggcctccta ttactgtcaa agtgttacca gcggtggaaa caattttata cggcgacaat 4200 ttaagtgata ttcttgcaac atcgacgaat tcctttttgg acattttagc atggttaccg 4260 tacggactat ttcattatgg ggccccattt gtcgttgctg ccatcttatt cgtatttggt 4320 ccaccaactg ttttgcaagg ttatgctttt gcatttggtt atatgaacct gtttggtgtt 4380 atcatgcaaa atgtctttcc agccgctccc ccatggtata aaattctcta tggattgcaa 4440 tcagccaact atgatatgca tggctcgcct ggtggattag ctagaattga taagctactc 4500 ggtattaata tgtatactac atgtttttca aattcctccg tcattttcgg tgcttttcct 4560 tcactgcatt ccgggtgtgc tactatggaa gccctgtttt tctgttattg ttttccaaaa 4620 ttgaagccct tgtttattgc ttatgtttgc tggttatggt ggtcaactat gtatctgaca 4680 caccattatt ttgtagacct tatggcaggt tctgtgctgt catacgttat tttccagtac 4740 acaaagtaca cacatttacc aattgtagat acatctcttt tttgcagatg gtcatacact 4800 tcaattgaga aatacgatat atcaaagagt gatccattgg ctgcagattc aaacgatatc 4860 gaaagtgtcc ctttgtccaa cttggaactt gactttgatc ttaatatgac tgatgaaccc 4920 agtgtaagcc cttcgttatt tgatggatct acttctgttt ctcgttcgtc cgccacgtct 4980 ataacgtcac taggtgtaaa gagggcttaa tgagtatttt atctgcaatt acggatacgg 5040 ttggtcttat gtagatacat ataaatatat atctttttct ttctttttct tagtcaggat 5100 tgtcgtttag cataatatac atgtagttta tttaatcaca taccactgat tatctttaga 5160 attttataaa tttttgaaat aaatgggtgg cttttaatgg tgtctatgtt aagtgaggct 5220 tttagaatgc tcttcctgct ttgtttatta tatgtgtatg aaagatatgt atgtatttac 5280 atgtgtttgt agcgtcccca gtcaaaacct gtgcgctata cctaaatgga ttgataatct 5340 tcattcacta attctaaaat agacttcttc cccaaagaac ggtgtaacga tgaggctcta 5400 tccagctgct tatctaaatc aactttaacg atggatgatc ttatgacacg gggatctttc 5460 tttaaagttc ttagaatttc agactgtacc gcagctgatg aatcaaacag cattaaaaag 5520 tgatatgctc gaaaatgttt ttcctggtct ttcttcatta ttttaggaag ataccttatg 5580 cccatgggta caatgtccct caccacacct ctgttttgaa taatcagttt cccgattgtg 5640 gaagacaatt cttttgcttc caactttggc gcattggagt tggttatgcg aacaagtccg 5700 atcagctcat aaagcatctt agtgaaaagg gtggttttgc gttattcttt cctctgttga 5760 agcttgcatg cctgcaggtc gactctagag gatccccggg taccgagctc gaattcactg 5820 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt 5880 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct 5940 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg 6000 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg 6060 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 6120 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 6180 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 6240 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga 6300 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 6360 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga 6420 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca 6480 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat 6540 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 6600 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 6660 tttcaccgtc atcaccgaaa cgcgcga 6687 // ID AB012283; SV 1; linear; other DNA; STD; SYN; 7104 BP. XX AC AB012283; XX DT 30-MAR-1998 (Rel. 55, Created) DT 18-AUG-2007 (Rel. 92, Last updated, Version 4) XX DE Shuttle vector pAUR112 gene for Aur1-C, complete cds. XX KW Aur1-C variant; aureobasidin resistant protein. XX OS Shuttle vector pAUR112 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-7104 RA Hashida-Okado T.; RT ; RL Submitted (18-MAR-1998) to the INSDC. RL Takashi Hashida-Okado, Takara Shuzo Co. ,Ltd., Biotechnology Research RL Laboratories; Seta 3-4-1, Otsu, Shiga 520-2193, Japan RL (E-mail:okadot@takara.co.jp, Tel:81-77-543-7298, Fax:81-77-543-2494) XX RN [2] RA Hashida-Okado T.; RT "Yeast-E. coli shuttle vector pAUR112"; RL Unpublished. XX DR MD5; af89c41863cda87870203b4fc45cb421. DR EuropePMC; PMC4357939; 25636849. XX FH Key Location/Qualifiers FH FT source 1..7104 FT /organism="Shuttle vector pAUR112" FT /mol_type="other DNA" FT /db_xref="taxon:75306" FT CDS 5128..6333 FT /codon_start=1 FT /transl_table=11 FT /gene="AUR1-C" FT /product="Aur1-C" FT /function="aureobasidin resistant" FT /note="variant" FT /protein_id="BAA25320.1" FT /translation="MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSDW FT VHYIFLGSIMLFVFITNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALYFT FT SSYFPDDRRPPITVKVLPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHYGAPFV FT VAAILFVFGPPTVLQGYAFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDMHGSP FT GGLARIDKLLGINMYTTCFSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPLFIAYV FT CWLWWSTMYLTHHYFVDLMAGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSIEKYDIS FT KSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATSITSLGVK FT RA" XX SQ Sequence 7104 BP; 1942 A; 1475 C; 1500 G; 2187 T; 0 other; gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 60 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 120 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 180 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 240 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 300 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 360 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 420 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 480 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 540 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 600 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 660 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 720 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 780 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga 840 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 900 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 960 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 1020 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 1080 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 1140 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 1200 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 1260 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 1320 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 1380 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 1440 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 1500 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 1560 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 1620 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 1680 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 1740 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 1800 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 1860 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 1920 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 1980 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 2040 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 2100 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 2160 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca 2220 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 2280 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg 2340 cagctgggcc attctcatga agaatatctt gaatttattg tcatattact agttggtgtg 2400 gaagtccata tatcggtgat caatatagtg gttgacatgc tggctagtca acattgagcc 2460 ttttgatcat gcaaatatat tacggtattt tacaatcaaa tatcaaactt aactattgac 2520 tttataactt atttaggtgg taacattctt ataaaaaaga aaaaaattac tgcaaaacag 2580 tactagcttt taacttgtat cctaggttat ctatgctgtc tcaccataga gaatattacc 2640 tatttcagaa tgtatgtcca tgattcgccg ggtaaataca tataatacac aaatctggct 2700 taataaagtc tataatatat ctcataaaga agtgctaaat tggctagtgc tatatatttt 2760 taagaaaatt tcttttgact aagtccatat cgactttgta aaagttcact ttagcataca 2820 tatattacac gagccagaaa ttgtaacttt tgcctaaaat cacaaattgc aaaatttaat 2880 tgcttgcaaa aggtcacatg cttataatca acttttttaa aaatttaaaa tactttttta 2940 ttttttattt ttaaacataa atgaaataat ttatttattg tttatgatta ccgaaacata 3000 aaacctgctc aagaaaaaga aactgttttg tccttggaaa aaaagcacta cctaggagcg 3060 gccaaaatgc cgaggctttc atagcttaaa ctctttacag aaaataggca ttatagatca 3120 gttcgagttt tcttattctt ccttccggtt ttatcgtcac agttttacag taaataagta 3180 tcacctctta gagttggcgg ttgtttgcaa gaccgagaaa aggctagcaa gaatcgggtc 3240 attgtagcgt atgcgcctgt gaacattctc ttcaacaagt ttgattccat tgcggtgaaa 3300 tggtaaaagt caaccccctg cgatgtatat tttcctgtac aatcaatcaa aaagccaaat 3360 gatttagcat tatctttaca tcttgttatt ttacagattt tatgtttaga tcttttatgc 3420 ttgcttttca aaaggcctgc aggcaagtgc acaaacaata cttaaataaa tactactcag 3480 taataaccta tttcttagca tttttgacga aatttgctat tttgttagag tcttttacac 3540 catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat 3600 caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctt ttcaattcat 3660 catttttttt ttattctttt ttttgatttc ggtttccttg aaattttttt gattcggtaa 3720 tctccgaaca gaaggaagaa cgaaggaagg agcacagact tagattggta tatatacgca 3780 tatgtagtgt tgaagaaaca tgaaattgcc cagtattctt aacccaactg cacagaacaa 3840 aaacctgcag gaaacgaaga taaatcatgt cgaaagctac atataaggaa cgtgctgcta 3900 ctcatcctag tcctgttgct gccaagctat ttaatatcat gcacgaaaag caaacaaact 3960 tgtgtgcttc attggatgtt cgtaccacca aggaattact ggagttagtt gaagcattag 4020 gtcccaaaat ttgtttacta aaaacacatg tggatatctt gactgatttt tccatggagg 4080 gcacagttaa gccgctaaag gcattatccg ccaagtacaa ttttttactc ttcgaagaca 4140 gaaaatttgc tgacattggt aatacagtca aattgcagta ctctgcgggt gtatacagaa 4200 tagcagaatg ggcagacatt acgaatgcac acggtgtggt gggcccaggt attgttagcg 4260 gtttgaagca ggcggcagaa gaagtaacaa aggaacctag aggccttttg atgttagcag 4320 aattgtcatg caagggctcc ctatctactg gagaatatac taagggtact gttgacattg 4380 cgaagagcga caaagatttt gttatcggct ttattgctca aagagacatg ggtggaagag 4440 atgaaggtta cgattggttg attatgacac ccggtgtggg tttagatgac aagggagacg 4500 cattgggtca acagtataga accgtggatg atgtggtctc tacaggatct gacattatta 4560 ttgttggaag aggactattt gcaaagggaa gggatgctaa ggtagagggt gaacgttaca 4620 gaaaagcagg ctgggaagca tatttgagaa gatgcggcca gcaaaactaa aaaactgtat 4680 tataagtaaa tgcatgtata ctaaactcac aaattagagc ttcaatttaa ttatatcagt 4740 tattacccgg gtaccgagct cgaatttcga gaaagtgccc atcagtgttc atatttgtta 4800 caaagtggtt ttctgatttg gtactactgc agaggcgtat tttttgcttc agttaccata 4860 gcgtaagaac actagcgact tttgttcgtg aaccaacaga gtaggatttc tactgctaca 4920 tctcttaggt agttggttag tccgatcgct cacttttggt tgttgttaag tacttcataa 4980 gtttatcctt ttcctttttc acactgagct actttgggta tagcttttgg cccaaggatc 5040 tttgaatttt ctccaaaagt actttatttt atatcctaca ggttgcggtt ttcatatttt 5100 aaaaagcttt taatcattcc tttgcgtatg gcaaaccctt tttcgagatg gtttctatca 5160 gagagacctc caaactgcca tgtagccgat ttagaaacaa gtttagatcc ccatcaaacg 5220 ttgttgaagg tgcaaaaata caaacccgct ttaagcgact gggtgcatta catcttcttg 5280 ggatccatca tgctgtttgt gttcattact aatcccgcac cttggatctt caagatcctt 5340 ttttattgtt tcttgggcac tttattcatc attccagcta cgtcacagtt tttcttcaat 5400 gccttgccca tcctaacatg ggtggcgctg tatttcactt catcgtactt tccagatgac 5460 cgcaggcctc ctattactgt caaagtgtta ccagcggtgg aaacaatttt atacggcgac 5520 aatttaagtg atattcttgc aacatcgacg aattcctttt tggacatttt agcatggtta 5580 ccgtacggac tatttcatta tggggcccca tttgtcgttg ctgccatctt attcgtattt 5640 ggtccaccaa ctgttttgca aggttatgct tttgcatttg gttatatgaa cctgtttggt 5700 gttatcatgc aaaatgtctt tccagccgct cccccatggt ataaaattct ctatggattg 5760 caatcagcca actatgatat gcatggctcg cctggtggat tagctagaat tgataagcta 5820 ctcggtatta atatgtatac tacatgtttt tcaaattcct ccgtcatttt cggtgctttt 5880 ccttcactgc attccgggtg tgctactatg gaagccctgt ttttctgtta ttgttttcca 5940 aaattgaagc ccttgtttat tgcttatgtt tgctggttat ggtggtcaac tatgtatctg 6000 acacaccatt attttgtaga ccttatggca ggttctgtgc tgtcatacgt tattttccag 6060 tacacaaagt acacacattt accaattgta gatacatctc ttttttgcag atggtcatac 6120 acttcaattg agaaatacga tatatcaaag agtgatccat tggctgcaga ttcaaacgat 6180 atcgaaagtg tccctttgtc caacttggaa cttgactttg atcttaatat gactgatgaa 6240 cccagtgtaa gcccttcgtt atttgatgga tctacttctg tttctcgttc gtccgccacg 6300 tctataacgt cactaggtgt aaagagggct taatgagtat tttatctgca attacggata 6360 cggttggtct tatgtagata catataaata tatatctttt tctttctttt tcttagtcag 6420 gattgtcgtt tagcataata tacatgtagt ttatttaatc acataccact gattatcttt 6480 agaattttat aaatttttga aataaatggg tggcttttaa tggtgtctat gttaagtgag 6540 gcttttagaa tgctcttcct gctttgttta ttatatgtgt atgaaagata tgtatgtatt 6600 tacatgtgtt tgtagcgtcc ccagtcaaaa cctgtgcgct atacctaaat ggattgataa 6660 tcttcattca ctaattctaa aatagacttc ttccccaaag aacggtgtaa cgatgaggct 6720 ctatccagct gcttatctaa atcaacttta acgatggatg atcttatgac acggggatct 6780 ttctttaaag ttcttagaat ttcagactgt accgcagctg atgaatcaaa cagcattaaa 6840 aagtgatatg ctcgaaaatg tttttcctgg tctttcttca ttattttagg aagatacctt 6900 atgcccatgg gtacaatgtc cctcaccaca cctctgtttt gaataatcag tttcccgatt 6960 gtggaagaca attcttttgc ttccaacttt ggcgcattgg agttggttat gcgaacaagt 7020 ccgatcagct cataaagcat cttagtgaaa agggtggttt tgcgttattc tttcctctgg 7080 atcctctaga gtcgacctcg aggc 7104 // ID AB012284; SV 1; linear; other DNA; STD; SYN; 6982 BP. XX AC AB012284; XX DT 30-MAR-1998 (Rel. 55, Created) DT 18-AUG-2007 (Rel. 92, Last updated, Version 4) XX DE Shuttle vector pAUR123 gene for Aur1-C, complete cds. XX KW Aur1-C variant; aureobasidin resistant protein. XX OS Shuttle vector pAUR123 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-6982 RA Hashida-Okado T.; RT ; RL Submitted (18-MAR-1998) to the INSDC. RL Takashi Hashida-Okado, Takara Shuzo Co. ,Ltd., Biotechnology Research RL Laboratories; Seta 3-4-1, Otsu, Shiga 520-2193, Japan RL (E-mail:okadot@takara.co.jp, Tel:81-77-543-7298, Fax:81-77-543-2494) XX RN [2] RA Hashida-Okado T.; RT "Yeast-E. coli shuttle vector pAUR123"; RL Unpublished. XX DR MD5; 4cf76abab465d9b3c5e2d66efaf072dd. XX FH Key Location/Qualifiers FH FT source 1..6982 FT /organism="Shuttle vector pAUR123" FT /mol_type="other DNA" FT /db_xref="taxon:75307" FT CDS 4017..5222 FT /codon_start=1 FT /transl_table=11 FT /gene="AUR1-C" FT /product="Aur1-C" FT /function="aureobasidin resistant" FT /note="variant" FT /protein_id="BAA25321.1" FT /translation="MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSDW FT VHYIFLGSIMLFVFITNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALYFT FT SSYFPDDRRPPITVKVLPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHYGAPFV FT VAAILFVFGPPTVLQGYAFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDMHGSP FT GGLARIDKLLGINMYTTCFSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPLFIAYV FT CWLWWSTMYLTHHYFVDLMAGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSIEKYDIS FT KSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATSITSLGVK FT RA" XX SQ Sequence 6982 BP; 1878 A; 1512 C; 1402 G; 2190 T; 0 other; gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 60 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 120 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 180 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 240 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 300 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 360 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 420 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 480 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 540 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 600 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 660 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 720 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 780 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga 840 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 900 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 960 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 1020 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 1080 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 1140 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 1200 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 1260 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 1320 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 1380 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct 1440 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 1500 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 1560 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 1620 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 1680 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 1740 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 1800 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 1860 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 1920 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 1980 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 2040 gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat 2100 ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca 2160 gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca 2220 cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg 2280 accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg 2340 cagctgggcc attctcatga agaatatctt gaatttattg tcatattact agttggtgtg 2400 gaagtccata tatcggtgat caatatagtg gttgacatgc tggctagtca acattgagcc 2460 ttttgatcat gcaaatatat tacggtattt tacaatcaaa tatcaaactt aactattgac 2520 tttataactt atttaggtgg taacattctt ataaaaaaga aaaaaattac tgcaaaacag 2580 tactagcttt taacttgtat cctaggttat ctatgctgtc tcaccataga gaatattacc 2640 tatttcagaa tgtatgtcca tgattcgccg ggtaaataca tataatacac aaatctggct 2700 taataaagtc tataatatat ctcataaaga agtgctaaat tggctagtgc tatatatttt 2760 taagaaaatt tcttttgact aagtccatat cgactttgta aaagttcact ttagcataca 2820 tatattacac gagccagaaa ttgtaacttt tgcctaaaat cacaaattgc aaaatttaat 2880 tgcttgcaaa aggtcacatg cttataatca acttttttaa aaatttaaaa tactttttta 2940 ttttttattt ttaaacataa atgaaataat ttatttattg tttatgatta ccgaaacata 3000 aaacctgctc aagaaaaaga aactgttttg tccttggaaa aaaagcacta cctaggagcg 3060 gccaaaatgc cgaggctttc atagcttaaa ctctttacag aaaataggca ttatagatca 3120 gttcgagttt tcttattctt ccttccggtt ttatcgtcac agttttacag taaataagta 3180 tcacctctta gagttggcgg ttgtttgcaa gaccgagaaa aggctagcaa gaatcgggtc 3240 attgtagcgt atgcgcctgt gaacattctc ttcaacaagt ttgattccat tgcggtgaaa 3300 tggtaaaagt caaccccctg cgatgtatat tttcctgtac aatcaatcaa aaagccaaat 3360 gatttagcat tatctttaca tcttgttatt ttacagattt tatgtttaga tcttttatgc 3420 ttgcttttca aaaggcctgc aggcaagtgc acaaacaata cttaaataaa tactactcag 3480 taataaccta tttcttagca tttttgacga aatttgctat tttgttagag tcttttacac 3540 catttgtctc cacacctccg cttacatcaa caccaataac gccatttaat ctaagcgcat 3600 caccaacatt ttctggcgtc agtccaccag ctaacataaa atgtaagctc gaatttcgag 3660 aaagtgccca tcagtgttca tatttgttac aaagtggttt tctgatttgg tactactgca 3720 gaggcgtatt ttttgcttca gttaccatag cgtaagaaca ctagcgactt ttgttcgtga 3780 accaacagag taggatttct actgctacat ctcttaggta gttggttagt ccgatcgctc 3840 acttttggtt gttgttaagt acttcataag tttatccttt tcctttttca cactgagcta 3900 ctttgggtat agcttttggc ccaaggatct ttgaattttc tccaaaagta ctttatttta 3960 tatcctacag gttgcggttt tcatatttta aaaagctttt aatcattcct ttgcgtatgg 4020 caaacccttt ttcgagatgg tttctatcag agagacctcc aaactgccat gtagccgatt 4080 tagaaacaag tttagatccc catcaaacgt tgttgaaggt gcaaaaatac aaacccgctt 4140 taagcgactg ggtgcattac atcttcttgg gatccatcat gctgtttgtg ttcattacta 4200 atcccgcacc ttggatcttc aagatccttt tttattgttt cttgggcact ttattcatca 4260 ttccagctac gtcacagttt ttcttcaatg ccttgcccat cctaacatgg gtggcgctgt 4320 atttcacttc atcgtacttt ccagatgacc gcaggcctcc tattactgtc aaagtgttac 4380 cagcggtgga aacaatttta tacggcgaca atttaagtga tattcttgca acatcgacga 4440 attccttttt ggacatttta gcatggttac cgtacggact atttcattat ggggccccat 4500 ttgtcgttgc tgccatctta ttcgtatttg gtccaccaac tgttttgcaa ggttatgctt 4560 ttgcatttgg ttatatgaac ctgtttggtg ttatcatgca aaatgtcttt ccagccgctc 4620 ccccatggta taaaattctc tatggattgc aatcagccaa ctatgatatg catggctcgc 4680 ctggtggatt agctagaatt gataagctac tcggtattaa tatgtatact acatgttttt 4740 caaattcctc cgtcattttc ggtgcttttc cttcactgca ttccgggtgt gctactatgg 4800 aagccctgtt tttctgttat tgttttccaa aattgaagcc cttgtttatt gcttatgttt 4860 gctggttatg gtggtcaact atgtatctga cacaccatta ttttgtagac cttatggcag 4920 gttctgtgct gtcatacgtt attttccagt acacaaagta cacacattta ccaattgtag 4980 atacatctct tttttgcaga tggtcataca cttcaattga gaaatacgat atatcaaaga 5040 gtgatccatt ggctgcagat tcaaacgata tcgaaagtgt ccctttgtcc aacttggaac 5100 ttgactttga tcttaatatg actgatgaac ccagtgtaag cccttcgtta tttgatggat 5160 ctacttctgt ttctcgttcg tccgccacgt ctataacgtc actaggtgta aagagggctt 5220 aatgagtatt ttatctgcaa ttacggatac ggttggtctt atgtagatac atataaatat 5280 atatcttttt ctttcttttt cttagtcagg attgtcgttt agcataatat acatgtagtt 5340 tatttaatca cataccactg attatcttta gaattttata aatttttgaa ataaatgggt 5400 ggcttttaat ggtgtctatg ttaagtgagg cttttagaat gctcttcctg ctttgtttat 5460 tatatgtgta tgaaagatat gtatgtattt acatgtgttt gtagcgtccc cagtcaaaac 5520 ctgtgcgcta tacctaaatg gattgataat cttcattcac taattctaaa atagacttct 5580 tccccaaaga acggtgtaac gatgaggctc tatccagctg cttatctaaa tcaactttaa 5640 cgatggatga tcttatgaca cggggatctt tctttaaagt tcttagaatt tcagactgta 5700 ccgcagctga tgaatcaaac agcattaaaa agtgatatgc tcgaaaatgt ttttcctggt 5760 ctttcttcat tattttagga agatacctta tgcccatggg tacaatgtcc ctcaccacac 5820 ctctgttttg aataatcagt ttcccgattg tggaagacaa ttcttttgct tccaactttg 5880 gcgcattgga gttggttatg cgaacaagtc cgatcagctc ataaagcatc ttagtgaaaa 5940 gggtggtttt gcgttattct ttcctctgga tcctctagct ccctaacatg taggtggcgg 6000 aggggagata tacaatagaa cagataccag acaagacata atgggctaaa caagactaca 6060 ccaattacac tgcctcattg atggtggtac ataacgaact aatactgtag ccctagactt 6120 gatagccatc atcatatcga agtttcacta ccctttttcc atttgccatc tattgaagta 6180 ataataggcg catgcaactt cttttctttt tttttctttt ctctctcccc cgttgttgtc 6240 tcaccatatc cgcaatgaca aaaaaatgat ggaagacact aaaggaaaaa attaacgaca 6300 aagacagcac caacagatgt cgttgttcca gagctgatga ggggtatctc gaagcacacg 6360 aaactttttc cttccttcat tcacgcacac tactctctaa tgagcaacgg tatacggcct 6420 tccttccagt tacttgaatt tgaaataaaa aaagtttgct gtcttgctat caagtataaa 6480 tagacctgca attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt 6540 gtttcttttt ctgcacaata tttcaagcta taccaagcat acaatcaact ggtacccggg 6600 tcgactcgag ctctagaggt taactaagcg aatttcttat gatttatgat ttttattatt 6660 aaataagtta taaaaaaaat aagtgtatac aaattttaaa gtgactctta ggttttaaaa 6720 cgaaaattct tattcttgag taactctttc ctgtaggtca ggttgctttc tcaggtatag 6780 catgaggtcg ctcttattga ccacatctct accggcatgc cgagcaaatg cctgcaaatc 6840 gctccccatt tcacccaatt gtagatatgc taactccagc aatgagttga tgaatctcgg 6900 tgtgtatttt atgtcctcag aggacaacac ctgttgtaat cgttcttcca cacggatcca 6960 cagcctagcc ttcagtcgag gc 6982 // ID AB012598; SV 1; linear; other DNA; STD; SYN; 45 BP. XX AC AB012598; XX DT 17-DEC-1998 (Rel. 58, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 3) XX DE Artificial sequence gene for BST-1 binding peptide, partial cds. XX KW BST-1 binding peptide. XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-45 RA Sato A.; RT ; RL Submitted (25-MAR-1998) to the INSDC. RL Atsushi Sato, Biomolecular Engineering Research Institute, Department of RL Molecular Biology; Furuedai 6-2-3, Suita, Osaka 565-0874, Japan RL (E-mail:satou@beri.co.jp, Tel:+81-6-872-8204, Fax:+81-6-872-8219) XX RN [2] RX DOI; 10.1042/0264-6021:3370491. RX PUBMED; 9895292. RA Sato A., Yamamoto S., Ishihara K., Hirano T., Jingami H.; RT "Novel peptide inhibitor of ecto-ADP-ribosyl cyclase of bone marrow stromal RT cell antigen-1 (BST-1/CD157)"; RL Biochem. J. 337:491-496(1999). XX DR MD5; 5189deb1f68dd0f630d812b7a8bde12e. XX FH Key Location/Qualifiers FH FT source 1..45 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS <1..>45 FT /codon_start=1 FT /transl_table=11 FT /product="BST-1 binding peptide" FT /protein_id="BAA34913.1" FT /translation="HSQISGKYQRYLKDA" XX SQ Sequence 45 BP; 13 A; 6 C; 13 G; 13 T; 0 other; catagtcaga ttagtgggaa gtatcagcgt tatctgaagg atgct 45 // ID AB012599; SV 1; linear; other DNA; STD; SYN; 45 BP. XX AC AB012599; XX DT 17-DEC-1998 (Rel. 58, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 3) XX DE Artificial sequence gene for BST-1 binding peptide, partial cds. XX KW BST-1 binding peptide. XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-45 RA Sato A.; RT ; RL Submitted (25-MAR-1998) to the INSDC. RL Atsushi Sato, Biomolecular Engineering Research Institute, Department of RL Molecular Biology; Furuedai 6-2-3, Suita, Osaka 565-0874, Japan RL (E-mail:satou@beri.co.jp, Tel:+81-6-872-8204, Fax:+81-6-872-8219) XX RN [2] RX DOI; 10.1042/0264-6021:3370491. RX PUBMED; 9895292. RA Sato A., Yamamoto S., Ishihara K., Hirano T., Jingami H.; RT "Novel peptide inhibitor of ecto-ADP-ribosyl cyclase of bone marrow stromal RT cell antigen-1 (BST-1/CD157)"; RL Biochem. J. 337:491-496(1999). XX DR MD5; 81ff3352da37c8992c7a7187a1a62b4b. XX FH Key Location/Qualifiers FH FT source 1..45 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS <1..>45 FT /codon_start=1 FT /transl_table=11 FT /product="BST-1 binding peptide" FT /protein_id="BAA34914.1" FT /translation="DDVVYTNIHKWGRRE" XX SQ Sequence 45 BP; 11 A; 4 C; 14 G; 16 T; 0 other; gatgatgttg tttatactaa tattcataag tggggtcgtc gggag 45 // ID AB013445; SV 1; linear; other DNA; STD; SYN; 159 BP. XX AC AB013445; XX DT 08-MAY-1998 (Rel. 55, Created) DT 01-JUN-2003 (Rel. 76, Last updated, Version 5) XX DE Synthetic construct gene for echistatin, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-159 RA Li H.C.; RT ; RL Submitted (29-APR-1998) to the INSDC. RL Hong C. Li, Abbott Laboratories, 47H-AP9A; One Abbott Park Road, Abbott RL Park, IL 60064-3500, U.S.A (E-mail:hong.li@abbott.com, Tel:1-847-938-0506, RL Fax:1-847-935-3585) XX RN [2] RX PUBMED; 8695184. RA Li H.C., Li X.B., Hu M.H.; RT "Site-directed mutagenesis of the gene of Echistatin Leu14-Lys15-Glu16"; RL Yi Chuan Xue Bao 23(2):163-168(1996). XX DR MD5; b4f001de0c19289b4592033fbdf253dc. XX FH Key Location/Qualifiers FH FT source 1..159 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 6..158 FT /codon_start=1 FT /transl_table=11 FT /product="echistatin" FT /protein_id="BAA25998.1" FT /translation="MECESGPCCRNCKFRGDGTICKRARGDDLDDYCNGKTCDCPRNPH FT KGPAT" XX SQ Sequence 159 BP; 42 A; 41 C; 43 G; 33 T; 0 other; aattcatgga atgcgaatca ggtccatgct gtcgtaactg caagttccgt ggggatggta 60 ccatctgtaa gcgcgcacgt ggtgatgatc tcgacgacta ctgcaacggt aagacctgtg 120 actgcccgag aaacccacac aagggtccag ctacctaag 159 // ID AB013921; SV 2; circular; other DNA; STD; SYN; 7570 BP. XX AC AB013921; XX DT 20-MAY-1998 (Rel. 55, Created) DT 10-MAR-2016 (Rel. 128, Last updated, Version 6) XX DE Cloning vector pKS145 DNA, complete sequence. XX KW . XX OS Cloning vector pKS145 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-7570 RA Terada Y., Park H.-S., Kuroki Y., Fujiyama A.; RT ; RL Submitted (06-MAY-1998) to the INSDC. RL Contact:Asao Fujiyama National Institute of Genetics, Human Genetics; Yata RL 1111, Mishima 411-8540, Japan XX RN [2] RA Terada Y., Park H.-S., Kuroki Y., Fujiyama A.; RT "Cloning vector pKS145"; RL Unpublished. XX DR MD5; 36f4db361c52948e48c53ed1130c9ac8. DR EuropePMC; PMC4625939; 26514418. XX FH Key Location/Qualifiers FH FT source 1..7570 FT /organism="Cloning vector pKS145" FT /mol_type="other DNA" FT /db_xref="taxon:76876" FT CDS join(7506..7570,1..238) FT /codon_start=1 FT /transl_table=11 FT /gene="lacZa" FT /product="beta-galactosidase alpha" FT /function="beta-galactosidase alpha donor" FT /note="contains a multiple cloning site incorporated into FT the 5' end of lacZ" FT /protein_id="BAH03500.1" FT /translation="MTMITPSARNTTHYRAAASMLESSADVVRREAFERVCIDHVDPAR FT SLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWKL" FT primer_bind complement(87..106) FT /note="pKS145_Fwd" FT regulatory complement(881..922) FT /note="contains the T4 gene 32 terminator and a portion of FT the polylinker from the omega interposon" FT /regulatory_class="terminator" FT CDS 940..1800 FT /codon_start=1 FT /transl_table=11 FT /gene="bla" FT /product="beta-lactamase" FT /function="ampicillin resistance" FT /protein_id="BAH03496.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT repeat_region complement(2596..3113) FT /function="plasmid partition centromere element" FT /rpt_family="SopB binding" FT /note="sopC" FT CDS complement(3186..4157) FT /codon_start=1 FT /transl_table=11 FT /gene="sopB" FT /product="partitioning protein" FT /function="plasmid partition" FT /protein_id="BAH03497.1" FT /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA FT ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP FT AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER FT GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD FT ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF FT APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP" FT CDS complement(4157..5323) FT /codon_start=1 FT /transl_table=11 FT /gene="sopA" FT /product="partitioning protein" FT /function="plasmid partition" FT /protein_id="BAH03498.1" FT /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGEA FT ADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAEDV FT FPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDLHIH FT AEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTDPHL FT MLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFFDML FT RDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDEVGK FT GQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR" FT repeat_region complement(5661..5908) FT /function="copy number control" FT /rpt_family="iteron, RepE binding" FT /note="incC" FT CDS complement(5911..6666) FT /codon_start=1 FT /transl_table=11 FT /gene="repE" FT /product="replication initiator protein" FT /protein_id="BAH03499.1" FT /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ FT IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG FT DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM FT RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM FT RLSYIEKKKGRQTTHIVFSFRDITSMTTG" FT primer_bind 7466..7486 FT /note="pKS145_Rev" XX SQ Sequence 7570 BP; 2106 A; 1843 C; 1784 G; 1837 T; 0 other; gagctccgcg gacgtcgtac gtcgcgaagc tttcgaacgc gtatgcatcg atcacgtgga 60 tccggcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac 120 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc 180 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatgga aattgtaagc 240 gttaatcccg ttccatacag aagctgggcg aacaaacgat gctcgccttc cagaaaaccg 300 aggatgcgaa ccacttcatc cggggtcagc accaccggca agcgccgcga cggccgaggt 360 cttccgatct cctgaagcca gggcagatcc gtgcacagca ccttgccgta gaagaacagc 420 aaggccgcca atgcctgacg atgcgtggag accgaaacct tgcgctcgtt cgccagccag 480 gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac accgtggaaa 540 cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag 600 tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc 660 gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg 720 gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac 780 gatgttacgc agcagggcag tcgccctaaa acaaagttaa acatcatgag ggaagcggtg 840 atcgccgaag tatcgactca actatcagag gtagttggcg gctggggtga tatttgatgc 900 gtcctgcatt cgtatgcggt cgattgaaaa aggaagagta tgagtattca acatttccgt 960 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 1020 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 1080 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 1140 agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag 1200 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 1260 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 1320 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 1380 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 1440 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg 1500 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 1560 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 1620 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 1680 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 1740 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 1800 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 1860 aaaaggatct aggtgaagat cctttttgat aatctcatgc gccgcttcgc ggcgcggctt 1920 aactcaagcg ttagatgcac taagcacata attgctcaca gccaaactat caggtcaagt 1980 ctgcttttat tatttttaag cgtgcataat aagccctaca caaattggga gatatatcat 2040 gaaaggctgg ctttttcttg ttatcgcaat agttggcgaa gtaatcgcaa catccgcatt 2100 aaaatctagc gagggcttta ctaagctgat ccggtggatg accttttgaa tgacctttaa 2160 tagattatat tactaattaa ttggggaccc tagaggtccc cttttttatt ttaaaaattt 2220 tttcacaaaa cggtttacaa gcataaagct gatccccggg aattctcctc tcacatgcgg 2280 cctccatcag gccgtgccgc acctgtaacc gggcgaccgc atacgaatgc aggtgcggcc 2340 ccgaagccgc agcgcgtgga ggagaaacga cgaaacgtga ccgggtgagg aggccgtcga 2400 cagcgacaca cttgcatcgg atgcagcccg gttaacgtgc cggcacggcc tgggtaacca 2460 ggtattttgt ccacataacc gtgcgcaaaa tgttgtggat aagcaggaca cagcagcaat 2520 ccacagcagg catacaaccg cacaccgagg ttactccgtt ctacaggtta cgacgacatg 2580 tcaatacttg cccttgacag gcattgatgg aatcgtagtc tcacgctgat agtctgatcg 2640 acaatacaag tgggaccgtg gtcccagacc gataatcaga ccgacaacac gagtgggatc 2700 gtggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata 2760 atcagaccga cgatacgagt gggaccgtgg ttccagacta ataatcagac cgacgatacg 2820 agtgggaccg tggtcccaga ctaataatca gaccgacgat acgagtggga ccatggtccc 2880 agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagtctga ttatcagacc 2940 gacgatacga gtgggaccgt ggtcccagac taataatcag accgacgata cgagtgggac 3000 cgtggtccca gactaataat cagaccgacg atacgagtgg gaccgtggtc ccagtctgat 3060 tatcagaccg acgatacaag tggaacagtg ggcccagaga gaatattcag gccagttatg 3120 ctttctggcc tgtaacaaag gacattaagt aaagacagat aaacgtagac taaaacgtgg 3180 tcgcatcagg gtgctggctt ttcaagttcc ttaagaatgg cctcaatttt ctctatacac 3240 tcagttggaa cacgagacct gtccaggtta agcaccattt tatcgccctt atacaatact 3300 gtcgctccag gagcaaactg atgtcgtgag cttaaactag ttcttgatgc agatgacgtt 3360 ttaagcacag aagttaaaag agtgataact tcttcagctt caaatatcac cccagctttt 3420 ttctgctcat gaaggttaga tgcctgctgc ttaagtaatt cctctttatc tgtaaaggct 3480 ttttgaagtg catcacctga ccgggcagat agttcaccgg ggtgagaaaa aagagcaaca 3540 actgatttag gcaatttggc ggtgttgata cagcgggtaa taatcttacg tgaaatattt 3600 tccgcatcag ccagcgcaga aatatttcca gcaaattcat tctgcaatcg gcttgcataa 3660 cgctgaccac gttcataagc acttgttggg cgataatcgt tacccaatct ggataatgca 3720 gccatctgct catcatccag ctcgccaacc agaacacgat aatcactttc ggtaagtgca 3780 gcagctttac gacggcgact cccatcggca atttctatga caccagatac tcttcgaccg 3840 aacgccggtg tctgttgacc agtcagtaga aaagaaggga tgagatcatc cagtgcgtcc 3900 tcagtaagca gctcctggtc acgttcatta cctgaccata cccgagaggt cttctcaaca 3960 ctatcacccc ggagcacttc aagagtaaac ttcacatccc gaccacatac aggcaaagta 4020 atggcattac cgcgagccat tactcctacg cgcgcaatta acgaatccac catcggggca 4080 gctggtgtcg ataacgaagt atcttcaacc ggttgagtat tgagcgtatg ttttggaata 4140 acaggcgcac gcttcattat ctaatctccc agcgtggttt aatcagacga tcgaaaattt 4200 cattgcagac aggttcccaa atagaaagag catttctcca ggcaccagtt gaagagcgtt 4260 gatcaatggc ctgttcaaaa acagttctca tccggatctg acctttacca acttcatccg 4320 tttcacgtac aacatttttt agaaccatgc ttccccaggc atcccgaatt tgctcctcca 4380 tccacgggga ctgagagcca ttactattgc tgtatttggt aagcaaaata cgtacatcag 4440 gctcgaaccc tttaagatca acgttcttga gcagatcacg aagcatatcg aaaaactgca 4500 gtgcggaggt gtagtcaaac aactcagcag gcgtgggaac aatcagcaca tcagcagcac 4560 atacgacatt aatcgtgccg atacccaggt taggcgcgct gtcaataact atgacatcat 4620 agtcatgagc aacagtttca atggccagtc ggagcatcag gtgtggatcg gtgggcagtt 4680 taccttcatc aaatttgccc attaactcag tttcaatacg gtgcagagcc agacaggaag 4740 gaataatgtc aagccccggc cagcaagtgg gctttattgc ataagtgaca tcgtcctttt 4800 ccccaagata gaaaggcagg agagtgtctt ctgcatgaat atgaagatct ggtacccatc 4860 cgtgatacat tgaggctgtt ccctgggggt cgttaccttc cacgagcaaa acacgtagcc 4920 ccttcagagc cagatcctga gcaagatgaa cagaaactga ggttttgtaa acgccacctt 4980 tatgggcagc aaccccgatc accggtggaa atacgtcttc agcacgtcgc aatcgcgtac 5040 caaacacatc acgcatatga ttaatttgtt caattgtata accaacacgt tgctcaaccc 5100 gtcctcgaat ttccatatcc gggtgcggta gtcgccctgc tttctcggca tctctgatag 5160 cctgagaaga aaccccaact aaatccgctg cttcacctat tctccagcgc cgggttattt 5220 tcctcgcttc cgggctgtca tcattaaact gtgcaatggc gatagccttc gtcatttcat 5280 gaccagcgtt tatgcactgg ttaagtgttt ccatgagttt cattctgaac atcctttaat 5340 cattgctttg cgttttttta ttaaatcttg caatttactg caaagcaaca acaaaatcgc 5400 aaagtcatca aaaaaccgca aagttgttta aaataagagc aacactacaa aaggagataa 5460 gaagagcaca tacctcagtc acttattatc actagcgctc gccgcagccg tgtaaccgag 5520 catagcgagc gaactggcga ggaagcaaag aagaactgtt ctgtcagata gctcttacgc 5580 tcagcgcaag aagaaatatc caccgtggga aaaactccag gtagaggtac acacgcggat 5640 agccaattca gagtaataaa ctgtgataat caaccctcat caatgatgac gaactaaccc 5700 ccgatatcag gtcacatgac gaagggaaag agaaggaaat caactgtgac aaactgccct 5760 caaatttggc ttccttaaaa attacagttc aaaaagtatg agaaaatcca tgcaggctga 5820 aggaaacagc aaaactgtga caaattaccc tcagtaggtc agaacaaatg tgacgaacca 5880 ccctcaaatc tgtgacagat aaccctcaga ctatcctgtc gtcatggaag tgatatcgcg 5940 gaaggaaaat acgatatgag tcgtctggcg gcctttcttt ttctcaatgt atgagaggcg 6000 cattggagtt ctgctgttga tctcattaac acagacctgc aggaagcggc ggcggaagtc 6060 aggcatacgc tggtaacttt gaggcagctg gtaacgctct atgatccagt cgattttcag 6120 agagacgatg cctgagccat ccggcttacg atactgacac agggattcgt ataaacgcat 6180 ggcatacgga ttggtgattt cttttgtttc actaagccga aactgcgtaa accggttctg 6240 taacccgata aagaagggaa tgagatatgg gttgatatgt acactgtaaa gccctctgga 6300 tggactgtgc gcacgtttga taaaccaagg aaaagattca tagccttttt catcgccggc 6360 atcctcttca gggcgataaa aaaccacttc cttccccgcg aaactcttca atgcctgccg 6420 tatatcctta ctggcttccg cagaggtcaa tccgaatatt tcagcatatt tagcaacatg 6480 gatctcgcag ataccgtcat gttcctgtag ggtgccatca gattttctga tctggtcaac 6540 gaacagatac agcatacgtt tttgatcccg ggagagacta tatgccgcct cagtgaggtc 6600 gtttgactgg acgattcgcg ggctattttt acgtttcttg tgattgataa ccgctgtttc 6660 cgccatgaca gatccatgtg aagtgtgaca agtttttaga ttgtcacact aaataaaaaa 6720 gagtcaataa gcagggataa ctttgtgaaa aaacagcttc ttctgagggc aatttgtcac 6780 agggttaagg gcaatttgtc acagacagga ctgtcatttg agggtgattt gtcacactga 6840 aagggcaatt tgtcacaaca ccttctctag aaccagcatg gataaaggcc tacaaggcgc 6900 tctaaaaaag aagatctaaa aactataaaa aaaataatta taaaaatatc cccgtggata 6960 agtggataac cccaagggaa gttttttcag gcatcgtgtg taagcagaat atataagtgc 7020 tgttccctgg tgcttcctcg ctcactcgaa attcccgggg atagctttat gcttgtaaac 7080 cgttttgtga aaaaattttt aaaataaaaa aggggacctc tagggtcccc aattaattag 7140 taatataatc tattaaaggt cattcaaaag gtcatccacc ggatcaattc ccctgctcgc 7200 gcaggctggg tgccaagctc tcgggtaaca tcaaggcccg atccttggag cccttgccct 7260 cccgcacgat gatcgtgccg tgatcgaaat ccagatcctt gacccgcagt tgcaaaccct 7320 cactgatccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag 7380 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt 7440 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 7500 cagctatgac catgattacg ccaagcgcgc gtaatacgac tcactatagg gcggccgcta 7560 gcatgctcga 7570 // ID AB014641; SV 1; circular; other DNA; STD; SYN; 3417 BP. XX AC AB014641; XX DT 06-JUN-1998 (Rel. 56, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 4) XX DE Cloning vector pGEX-PUC-3T DNA, complete sequence. XX KW cloning vector; GST-fusion protein; GST-stuffer fusion protein. XX OS Cloning vector pGEX-PUC-3T OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3417 RA Fukunaga R.; RT ; RL Submitted (22-MAY-1998) to the INSDC. RL Rikiro Fukunaga, Osaka University Medical School, Department of Genetics RL B-3; 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan RL (E-mail:fukunaga@genetic.med.osaka-u.ac.jp, Tel:81-6-879-3318, RL Fax:81-6-879-3319) XX RN [2] RX DOI; 10.1093/emboj/16.8.1921. RX PUBMED; 9155018. RA Fukunaga R., Hunter T.; RT "MNK1, a new MAP kinase-activated protein kinase, isolated by a novel RT expression screening method for identifying protein kinase substrates"; RL EMBO J. 16(8):1921-1933(1997). XX DR MD5; 9ba7eb1bd1dfe6ab6796eac21bf50d00. XX FH Key Location/Qualifiers FH FT source 1..3417 FT /organism="Cloning vector pGEX-PUC-3T" FT /mol_type="other DNA" FT /db_xref="taxon:77040" FT misc_feature 9..1997 FT /note="pUC119" FT misc_feature 2005..2953 FT /note="pGEX-KG" FT CDS 2246..3142 FT /codon_start=1 FT /transl_table=11 FT /gene="GST-Stuffer fusion" FT /product="GST-stuffer fusion protein" FT /protein_id="BAA28713.1" FT /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FT FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY FT GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL FT YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLV FT PRGSPGISGGGGGIGPAGLEGILLHDCGHPGAQAAVSWVWDTWPEGILPVGAATEPGSS FT TEAGEQQQEAEQAQDDV" FT misc_feature 2966..3385 FT /note="Stuffer:that the stuffer DNA is derived from the FT 420bp PvuII fragment(1802-2221) of the human G-CSF receptor FT cDNA (Acc# No.M38025)" XX SQ Sequence 3417 BP; 864 A; 781 C; 898 G; 874 T; 0 other; gcggccgctc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 60 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 120 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 180 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 240 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 300 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 360 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 420 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 480 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 540 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 600 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 660 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 720 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 780 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 840 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 900 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 960 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 1020 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 1080 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 1140 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 1200 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 1260 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 1320 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 1380 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 1440 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 1500 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 1560 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 1620 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 1680 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 1740 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 1800 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 1860 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 1920 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1980 cgattcatta atgcagcctc gaggtctgca ccaatgcttc tggcgtcagg cagccatcgg 2040 aagctgtggt atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc 2100 actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg 2160 aaatgagctg ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa 2220 caatttcaca caggaaacag tattcatgtc ccctatacta ggttattgga aaattaaggg 2280 ccttgtgcaa cccactcgac ttcttttgga atatcttgaa gaaaaatatg aagagcattt 2340 gtatgagcgc gatgaaggtg ataaatggcg aaacaaaaag tttgaattgg gtttggagtt 2400 tcccaatctt ccttattata ttgatggtga tgttaaatta acacagtcta tggccatcat 2460 acgttatata gctgacaagc acaacatgtt gggtggttgt ccaaaagagc gtgcagagat 2520 ttcaatgctt gaaggagcgg ttttggatat tagatacggt gtttcgagaa ttgcatatag 2580 taaagacttt gaaactctca aagttgattt tcttagcaag ctacctgaaa tgctgaaaat 2640 gttcgaagat cgtttatgtc ataaaacata tttaaatggt gatcatgtaa cccatcctga 2700 cttcatgttg tatgacgctc ttgatgttgt tttatacatg gacccaatgt gcctggatgc 2760 gttcccaaaa ttagtttgtt ttaaaaaacg tattgaagct atcccacaaa ttgataagta 2820 cttgaaatcc agcaagtata tagcatggcc tttgcagggc tggcaagcca cgtttggtgg 2880 tggcgaccat cctccaaaat cggatctggt tccgcgtgga tccccgggaa tttccggtgg 2940 tggtggtgga attggccctg caggcctgga aggcatcctc ctccatgatt gtgggcaccc 3000 aggagcccag gctgctgtga gctgggtctg ggacacttgg ccagagggga ttcttcctgt 3060 tggggctgca acagagccag gcagttccac agaggcaggt gagcaacagc aggaggccga 3120 acaggcccag gatgatgtgt agctccgacc cctctggggt caaggtcatc agggtgagga 3180 ctgtactgtt ggtggcccca gcctggctgg cagccatgag gtggatgtga tacagactgg 3240 cgggctccag gccatggagg acaaagccac gggaggaggc attcaggatg gcggagaagg 3300 actggttctg agcgttggtc cagaagatgg tgtagtgggt aagggggctc ttccccagct 3360 cagggggctc aggcacccac tccaggcctg cagggccaat tctagatgac taagctt 3417 // ID AB015619; SV 1; circular; other DNA; STD; SYN; 7564 BP. XX AC AB015619; XX DT 06-APR-1999 (Rel. 59, Created) DT 17-JUL-2009 (Rel. 101, Last updated, Version 4) XX DE Cloning vector pCHR8 DNA, complete sequence. XX KW . XX OS Cloning vector pCHR8 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-7564 RA Miyazaki C.; RT ; RL Submitted (16-JUN-1998) to the INSDC. RL Contact:Chikara Miyazaki Kyushu National Agricultural Experiment Station, RL Department of Crop Breeding; 2421 Suya, Nishigoshi, Kumamoto 861-1192, RL Japan XX RN [2] RA Miyazaki C., Saito A.; RT "Construction of a rice bacterial artificial chromosome library using a new RT vector suitable for map-based cloning and identification of clones linked RT to lethal factor locus in rice chromosome 3"; RL Unpublished. XX DR MD5; 25f10b90fce4d20bc553f6f0d67cacbb. XX FH Key Location/Qualifiers FH FT source 1..7564 FT /organism="Cloning vector pCHR8" FT /mol_type="other DNA" FT /note="pCHR8 is aderivative of BAC cloning vector, pBelo FT BAC11: related sequence U51113" FT /db_xref="taxon:78256" FT misc_feature 333..440 FT /note="multicloning site from EcoRI to MluI" FT CDS complement(823..1482) FT /codon_start=1 FT /transl_table=11 FT /product="chloramphenicol acetyltransferase" FT /protein_id="BAA76493.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS 2822..3577 FT /codon_start=1 FT /transl_table=11 FT /product="29kD protein essential for the replication of FT mini F plasmid" FT /protein_id="BAA76494.1" FT /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ FT IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG FT DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM FT RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM FT RLSYIEKKKGRQTTHIVFSFRDITSMTTG" FT misc_feature complement(7223..7312) FT /note="cosN site" FT misc_feature 7524..7557 FT /note="loxP site" XX SQ Sequence 7564 BP; 1879 A; 1699 C; 1848 G; 2138 T; 0 other; gcggccgcaa ggggttcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 60 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 120 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 180 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 240 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 300 ccagtgaatt gtaatacgac tcactatagg gcgaattcga gctcggtacc cgggggcgcg 360 ccggatcctt aattaagtct agagtcgatt taaatgactg tttaaacctg caggcatgca 420 agcttgggct agctacgcgt gagcttgagt attctatagt gtcacctaaa tagcttggcg 480 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac 540 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca 600 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat 660 taatgaatcg gccaacgcga accccttgcg gccgcccggg ccgtcgacca attctcatgt 720 ttgacagctt atcatcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta 780 gcaaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca 840 ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc catcacaaac 900 ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt 960 gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact 1020 ggtgaaactc acccagggat tggctgagac gaaaaacata ttctcaataa accctttagg 1080 gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg 1140 ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa 1200 aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat 1260 acggaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa 1320 cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg 1380 gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg 1440 ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc 1500 tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa 1560 gttggaacct cttacgtgcc gatcaacgtc tcattttcgc caaaagttgg cccagggctt 1620 cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg 1680 tatttattcg cgataagctc atggagcggc gtaaccgtcg cacaggaagg acagagaaag 1740 cgcggatctg ggaagtgacg gacagaacgg tcaggacctg gattggggag gcggttgccg 1800 ccgctgctgc tgacggtgtg acgttctctg ttccggtcac accacatacg ttccgccatt 1860 cctatgcgat gcacatgctg tatgccggta taccgctgaa agttctgcaa agcctgatgg 1920 gacataagtc catcagttca acggaagtct acacgaaggt ttttgcgctg gatgtggctg 1980 cccggcaccg ggtgcagttt gcgatgccgg agtctgatgc ggttgcgatg ctgaaacaat 2040 tatcctgaga ataaatgcct tggcctttat atggaaatgt ggaactgagt ggatatgctg 2100 tttttgtctg ttaaacagag aagctggctg ttatccactg agaagcgaac gaaacagtcg 2160 ggaaaatctc ccattatcgt agagatccgc attattaatc tcaggagcct gtgtagcgtt 2220 tataggaagt agtgttctgt catgatgcct gcaagcggta acgaaaacga tttgaatatg 2280 ccttcaggaa caatagaaat cttcgtgcgg tgttacgttg aagtggagcg gattatgtca 2340 gcaatggaca gaacaaccta atgaacacag aaccatgatg tggtctgtcc ttttacagcc 2400 agtagtgctc gccgcagtcg agcgacaggg cgaagccctc gagtgagcga ggaagcacca 2460 gggaacagca cttatatatt ctgcttacac acgatgcctg aaaaaacttc ccttggggtt 2520 atccacttat ccacggggat atttttataa ttattttttt tatagttttt agatcttctt 2580 ttttagagcg ccttgtaggc ctttatccat gctggttcta gagaaggtgt tgtgacaaat 2640 tgccctttca gtgtgacaaa tcaccctcaa atgacagtcc tgtctgtgac aaattgccct 2700 taaccctgtg acaaattgcc ctcagaagaa gctgtttttt cacaaagtta tccctgctta 2760 ttgactcttt tttatttagt gtgacaatct aaaaacttgt cacacttcac atggatctgt 2820 catggcggaa acagcggtta tcaatcacaa gaaacgtaaa aatagcccgc gaatcgtcca 2880 gtcaaacgac ctcactgagg cggcatatag tctctcccgg gatcaaaaac gtatgctgta 2940 tctgttcgtt gaccagatca gaaaatctga tggcacccta caggaacatg acggtatctg 3000 cgagatccat gttgctaaat atgctgaaat attcggattg acctctgcgg aagccagtaa 3060 ggatatacgg caggcattga agagtttcgc ggggaaggaa gtggtttttt atcgccctga 3120 agaggatgcc ggcgatgaaa aaggctatga atcttttcct tggtttatca aacgtgcgca 3180 cagtccatcc agagggcttt acagtgtaca tatcaaccca tatctcattc ccttctttat 3240 cgggttacag aaccggttta cgcagtttcg gcttagtgaa acaaaagaaa tcaccaatcc 3300 gtatgccatg cgtttatacg aatccctgtg tcagtatcgt aagccggatg gctcaggcat 3360 cgtctctctg aaaatcgact ggatcataga gcgttaccag ctgcctcaaa gttaccagcg 3420 tatgcctgac ttccgccgcc gcttcctgca ggtctgtgtt aatgagatca acagcagaac 3480 tccaatgcgc ctctcataca ttgagaaaaa gaaaggccgc cagacgactc atatcgtatt 3540 ttccttccgc gatatcactt ccatgacgac aggatagtct gagggttatc tgtcacagat 3600 ttgagggtgg ttcgtcacat ttgttctgac ctactgaggg taatttgtca cagttttgct 3660 gtttccttca gcctgcatgg attttctcat actttttgaa ctgtaatttt taaggaagcc 3720 aaatttgagg gcagtttgtc acagttgatt tccttctctt tcccttcgtc atgtgacctg 3780 atatcggggg ttagttcgtc atcattgatg agggttgatt atcacagttt attactctga 3840 attggctatc cgcgtgtgta cctctacctg gagtttttcc cacggtggat atttcttctt 3900 gcgctgagcg taagagctat ctgacagaac agttcttctt tgcttcctcg ccagttcgct 3960 cgctatgctc ggttacacgg ctgcggcgag cgctagtgat aataagtgac tgaggtatgt 4020 gctcttctta tctccttttg tagtgttgct cttattttaa acaactttgc ggttttttga 4080 tgactttgcg attttgttgt tgctttgcag taaattgcaa gatttaataa aaaaacgcaa 4140 agcaatgatt aaaggatgtt cagaatgaaa ctcatggaaa cacttaacca gtgcataaac 4200 gctggtcatg aaatgacgaa ggctatcgcc attgcacagt ttaatgatga cagcccggaa 4260 gcgaggaaaa taacccggcg ctggagaata ggtgaagcag cggatttagt tggggtttct 4320 tctcaggcta tcagagatgc cgagaaagca gggcgactac cgcacccgga tatggaaatt 4380 cgaggacggg ttgagcaacg tgttggttat acaattgaac aaattaatca tatgcgtgat 4440 gtgtttggta cgcgattgcg acgtgctgaa gacgtatttc caccggtgat cggggttgct 4500 gcccataaag gtggcgttta caaaacctca gtttctgttc atcttgctca ggatctggct 4560 ctgaaggggc tacgtgtttt gctcgtggaa ggtaacgacc cccagggaac agcctcaatg 4620 tatcacggat gggtaccaga tcttcatatt catgcagaag acactctcct gcctttctat 4680 cttggggaaa aggacgatgt cacttatgca ataaagccca cttgctggcc ggggcttgac 4740 attattcctt cctgtctggc tctgcaccgt attgaaactg agttaatggg caaatttgat 4800 gaaggtaaac tgcccaccga tccacacctg atgctccgac tggccattga aactgttgct 4860 catgactatg atgtcatagt tattgacagc gcgcctaacc tgggtatcgg cacgattaat 4920 gtcgtatgtg ctgctgatgt gctgattgtt cccacgcctg ctgagttgtt tgactacacc 4980 tccgcactgc agtttttcga tatgcttcgt gatctgctca agaacgttga tcttaaaggg 5040 ttcgagcctg atgtacgtat tttgcttacc aaatacagca atagtaatgg ctctcagtcc 5100 ccgtggatgg aggagcaaat tcgggatgcc tggggaagca tggttctaaa aaatgttgta 5160 cgtgaaacgg atgaagttgg taaaggtcag atccggatga gaactgtttt tgaacaggcc 5220 attgatcaac gctcttcaac tggtgcctgg agaaatgctc tttctatttg ggaacctgtc 5280 tgcaatgaaa ttttcgatcg tctgattaaa ccacgctggg agattagata atgaagcgtg 5340 cgcctgttat tccaaaacat acgctcaata ctcaaccggt tgaagatact tcgttatcga 5400 caccagctgc cccgatggtg gattcgttaa ttgcgcgcgt aggagtaatg gctcgcggta 5460 atgccattac tttgcctgta tgtggtcggg atgtgaagtt tactcttgaa gtgctccggg 5520 gtgatagtgt tgagaagacc tctcgggtat ggtcaggtaa tgaacgtgac caggagctgc 5580 ttactgagga cgcactggat gatctcatcc cttcttttct actgactggt caacagacac 5640 cggcgttcgg tcgaagagta tctggtgtca tagaaattgc cgatgggagt cgccgtcgta 5700 aagctgctgc acttaccgaa agtgattatc gtgttctggt tggcgagctg gatgatgagc 5760 agatggctgc attatccaga ttgggtaacg attatcgccc aacaagtgct tatgaacgtg 5820 gtcagcgtta tgcaagccga ttgcagaatg aatttgctgg aaatatttct gcgctggctg 5880 atgcggaaaa tatttcacgt aagattatta cccgctgtat caacaccgcc aaattgccta 5940 aatcagttgt tgctcttttt tctcaccccg gtgaactatc tgcccggtca ggtgatgcac 6000 ttcaaaaagc ctttacagat aaagaggaat tacttaagca gcaggcatct aaccttcatg 6060 agcagaaaaa agctggggtg atatttgaag ctgaagaagt tatcactctt ttaacttctg 6120 tgcttaaaac gtcatctgca tcaagaacta gtttaagctc acgacatcag tttgctcctg 6180 gagcgacagt attgtataag ggcgataaaa tggtgcttaa cctggacagg tctcgtgttc 6240 caactgagtg tatagagaaa attgaggcca ttcttaagga acttgaaaag ccagcaccct 6300 gatgcgacca cgttttagtc tacgtttatc tgtctttact taatgtcctt tgttacaggc 6360 cagaaagcat aactggcctg aatattctct ctgggcccac tgttccactt gtatcgtcgg 6420 tctgataatc agactgggac cacggtccca ctcgtatcgt cggtctgatt attagtctgg 6480 gaccacggtc ccactcgtat cgtcggtctg attattagtc tgggaccacg gtcccactcg 6540 tatcgtcggt ctgataatca gactgggacc acggtcccac tcgtatcgtc ggtctgatta 6600 ttagtctggg accatggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg 6660 tcccactcgt atcgtcggtc tgattattag tctggaacca cggtcccact cgtatcgtcg 6720 gtctgattat tagtctggga ccacggtccc actcgtatcg tcggtctgat tattagtctg 6780 ggaccacgat cccactcgtg ttgtcggtct gattatcggt ctgggaccac ggtcccactt 6840 gtattgtcga tcagactatc agcgtgagac tacgattcca tcaatgcctg tcaagggcaa 6900 gtattgacat gtcgtcgtaa cctgtagaac ggagtaacct cggtgtgcgg ttgtatgcct 6960 gctgtggatt gctgctgtgt cctgcttatc cacaacattt tgcgcacggt tatgtggaca 7020 aaatacctgg ttacccaggc cgtgccggca cgttaaccgg gctgcatccg atgcaagtgt 7080 gtcgctgtcg acgagctcgc gagctcggac atgaggttgc cccgtattca gtgtcgctga 7140 tttgtattgt ctgaagttgt ttttacgtta agttgatgca gatcaattaa tacgatacct 7200 gcgtcataat tgattatttg acgtggtttg atggcctcca cgcacgttgt gatatgtaga 7260 tgataatcat tatcacttta cgggtccttt ccggtgatcc gacaggttac ggggcggcga 7320 cctcgcgggt tttcgctatt tatgaaaatt ttccggttta aggcgtttcc gttcttcttc 7380 gtcataactt aatgttttta tttaaaatac cctctgaaaa gaaaggaaac gacaggtgct 7440 gaaagcgagc tttttggcct ctgtcgtttc ctttctctgt ttttgtccgt ggaatgaaca 7500 atggaagtcc gagctcatcg ctaataactt cgtatagcat acattatacg aagttatatt 7560 cgat 7564 // ID AB019512; SV 1; linear; other DNA; STD; SYN; 786 BP. XX AC AB019512; XX DT 04-JUL-1999 (Rel. 60, Created) DT 27-FEB-2006 (Rel. 86, Last updated, Version 5) XX DE Synthetic construct homo sapiens gene for single chain Fv antibody, partial DE cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-786 RA Wang L., Zhao D.; RT ; RL Submitted (01-NOV-1998) to the INSDC. RL Lin Wang, Changchun Institute of Applied Chemistry, Lab of Rear Earth RL Chemistry and Physics; Renmin Street, Changchun 130022, P.R.China RL (E-mail:wangdx@public.jl.cninfo.net, Tel:86-0431-5682801(ex.5366)) XX RN [2] RX DOI; 10.1016/0022-2836(92)90223-7. RX PUBMED; 1404388. RA Tomlinson I.m., Walter G., Marks J.d., Llewelyn M.b., Winter G.; RT "The repertoire of human germline VH sequences reveals about fifty groups RT of VH segments with different hypervariable loops"; RL J. Mol. Biol. 227(3):776-798(1992). XX DR MD5; 8102d61ad9ec2baa4762e307bd23355f. DR IMGT/LIGM; AB019512. XX FH Key Location/Qualifiers FH FT source 1..786 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /clone_lib="human semi-synthetic scFv antibody library" FT /clone="pHENI" FT /db_xref="taxon:32630" FT CDS 1..>786 FT /codon_start=1 FT /transl_table=11 FT /product="single chain Fv antibody" FT /protein_id="BAA82041.1" FT /translation="MAQVQLVQSGAEVKKPGASVKVSCKASGYTFTSYAMHWVRQAPGQ FT RLEWMGWINAGNGNTKYSQKFQGRVTITRDTSASTAYMELSSLRSEDTAVYYCARLTPN FT KFKSRGHWGQGTLVTVSRGGGGSGGGGSGGGGSSELTQDPAVSVALGQTVRITCQGDSL FT RSYYASWYQQKPGQAPVLVIYGKNNRPSGIPDRFSGSSSGNTASLTITGAQAEDEADYY FT CNSRDSSGNHVVFGGGTKLTVLGAAAEQKLISEEDLNGAA" XX SQ Sequence 786 BP; 187 A; 198 C; 245 G; 156 T; 0 other; atggcccagg tgcagctggt gcagtctggg gctgaggtga agaagcctgg ggcctcagtg 60 aaggtttcct gcaaggcttc tggatacacc ttcactagct atgctatgca ttgggtgcgc 120 caggcccccg gacaaaggct tgagtggatg ggatggatca acgctggcaa tggtaacaca 180 aaatattcac agaagttcca gggcagagtc accattacca gggacacatc cgcgagcaca 240 gcctacatgg agctgagcag cctgagatct gaagacacgg ccgtgtatta ctgtgcaagg 300 ttgacgccta ataagtttaa gtcgcgtggt cattggggcc aaggtaccct ggtcaccgtg 360 tcgagaggtg gcggtggctc gggcggtggt gggtcgggtg gcggcggatc ttctgagctg 420 actcaggacc ctgctgtgtc tgtggccttg ggacagacag tcaggatcac atgccaagga 480 gacagcctca gaagctatta tgcaagctgg taccagcaga agccaggaca ggcccctgta 540 cttgtcatct atggtaaaaa caaccggccc tcagggatcc cagaccgatt ctctggctcc 600 agctcaggaa acacagcttc cttgaccatc actggggctc aggcggaaga tgaggctgac 660 tattactgta actcccggga cagcagtggt aaccatgtgg tattcggcgg agggaccaag 720 ctgaccgtcc taggtgcggc cgcagaacaa aaactcatct cagaagagga tctgaatggg 780 gccgca 786 // ID AB019603; SV 1; circular; other DNA; STD; SYN; 2972 BP. XX AC AB019603; XX DT 04-DEC-1998 (Rel. 57, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 6) XX DE Cloning vector pTH18kr DNA, complete sequence. XX KW . XX OS Cloning vector pTH18kr OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-2972 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; 8eb8de784ccd07bab2f6fb3a5a191b07. XX CC The copy number of pKF28wt is 5 per chromosome. CC Drug concentration for kanamycin selection is 20 microgramms per CC ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for alpha-complemetation selection. CC The multiple cloning sites are as in pKF18k (Gene, 1995; 167, CC 333-334). XX FH Key Location/Qualifiers FH FT source 1..2972 FT /organism="Cloning vector pTH18kr" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84726" FT CDS 1..309 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF18k" FT /protein_id="BAA34741.1" FT /translation="MTMITPNSSSVPGDPLESTCRHASLLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF" FT CDS complement(310..1125) FT /codon_start=1 FT /transl_table=11 FT /gene="agp-5" FT /product="aminoglucoside phosphotransferase" FT /protein_id="BAA34742.1" FT /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT CDS 1815..2765 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="wild type repA in pSC101" FT /protein_id="BAA34743.1" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" XX SQ Sequence 2972 BP; 950 A; 634 C; 595 G; 793 T; 0 other; atgaccatga ttacgccgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc 60 aggcatgcaa gcttactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg cgatttattc 240 aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac 300 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 360 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 420 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 480 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg 540 agtgacgact gaatccggtg agaatggcaa gagcttatgc atttctttcc agacttgttc 600 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat 660 tcgtgactgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac 720 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga 780 atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag tggtgagtaa 840 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt 900 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg 960 tttcagaaac aactctggcg catcgggctt cccatacaac cgatagattg tcgcacctga 1020 ttgcccgaca ttatcgcgcg cccatttata cccatataaa tcagcatcca tgttggaatt 1080 taatcgcggc ttcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt 1140 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat 1200 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga 1260 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgc tgtagtgcca 1320 tttaccccca ttcactgcca gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca 1380 gcgactcagg tgcctgatgg tcggagacaa aaggaatatt cagcgatttg cccgagcttg 1440 cgagggtgct acttaagcct ttagggtttt aaggtctgtt ttgtagagga gcaaacagcg 1500 tttgcgacat ccttttgtaa tactgcggaa ctgactaaag tagtgagtta tacacagggc 1560 tgggatctat tctttttatc tttttttatt ctttctttat tctataaatt ataaccactt 1620 gaatataaac aaaaaaaaca cacaaaggtc tagcggaatt tacagagggt ctagcagaat 1680 ttacaagttt tccagcaaag gtctagcaga atttacagat acccacaact caaaggaaaa 1740 ggactagtaa ttatcattga ctagcccatc tcaattggta tagtgattaa aatcacctag 1800 accaattgag atgtatgtct gaattagttg ttttcaaagc aaatgaacta gcgattagtc 1860 gctatgactt aacggagcat gaaaccaagc taattttatg ctgtgtggca ctactcaacc 1920 ccacgattga aaaccctaca aggaaagaac ggacggtatc gttcacttat aaccaatacg 1980 ctcagatgat gaacatcagt agggaaaatg cttatggtgt attagctaaa gcaaccagag 2040 agctgatgac gagaactgtg gaaatcagga atcctttggt taaaggcttt gagattttcc 2100 agtggacaaa ctatgccaag ttctcaagcg aaaaattaga attagttttt agtgaagaga 2160 tattgcctta tcttttccag ttaaaaaaat tcataaaata taatctggaa catgttaagt 2220 cttttgaaaa caaatactct atgaggattt atgagtggtt attaaaagaa ctaacacaaa 2280 agaaaactca caaggcaaat atagagatta gccttgatga atttaagttc atgttaatgc 2340 ttgaaaataa ctaccatgag tttaaaaggc ttaaccaatg ggttttgaaa ccaataagta 2400 aagatttaaa cacttacagc aatatgaaat tggtggttga taagcgaggc cgcccgactg 2460 atacgttgat tttccaagtt gaactagata gacaaatgga tctcgtaacc gaacttgaga 2520 acaaccagat aaaaatgaat ggtgacaaaa taccaacaac cattacatca gattcctacc 2580 tacataacgg actaagaaaa acactacacg atgctttaac tgcaaaaatt cagctcacca 2640 gttttgaggc aaaatttttg agtgacatgc aaagtaagta tgatctcaat ggttcgttct 2700 catggctcac gcaaaaacaa cgaaccacac tagagaacat actggctaaa tacggaagga 2760 tctgaggttc ttatggctct tgtatctatc agtgaagcat caagactaac aaacaaaagt 2820 agaacaactg ttcaccgtta catatcaaag ggaaaactgt ccataatgtg agttagctca 2880 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 2940 tgagcggata acaatttcac acaggaaaac at 2972 // ID AB019604; SV 1; circular; other DNA; STD; SYN; 2972 BP. XX AC AB019604; XX DT 04-DEC-1998 (Rel. 57, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 8) XX DE Cloning vector pTH18ks1 DNA, complete sequence. XX KW . XX OS Cloning vector pTH18ks1 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-2972 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; 5a601e4147eb107cb9bfec593d364e78. XX CC The copy number of pKF28ts1 is 14 per chromosome. CC Drug concentration for kanamycin selection is 20 microgramms per CC ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for CC alpha-complemetation selection. CC The multiple cloning sites are as in pKF18k (Gene, 1995; 167, CC 333-334). XX FH Key Location/Qualifiers FH FT source 1..2972 FT /organism="Cloning vector pTH18ks1" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84727" FT CDS 1..309 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF18k" FT /protein_id="BAA34744.1" FT /translation="MTMITPNSSSVPGDPLESTCRHASLLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF" FT CDS complement(310..1125) FT /codon_start=1 FT /transl_table=11 FT /gene="agp-5" FT /product="aminoglucoside phosphotransferase" FT /protein_id="BAA34745.1" FT /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT CDS 1815..2765 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="repAts1 in pHSG1" FT /protein_id="BAA34746.1" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" FT variation 1981 FT /replace="c" XX SQ Sequence 2972 BP; 950 A; 633 C; 595 G; 794 T; 0 other; atgaccatga ttacgccgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc 60 aggcatgcaa gcttactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg cgatttattc 240 aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac 300 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 360 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 420 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 480 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg 540 agtgacgact gaatccggtg agaatggcaa gagcttatgc atttctttcc agacttgttc 600 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat 660 tcgtgactgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac 720 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga 780 atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag tggtgagtaa 840 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt 900 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg 960 tttcagaaac aactctggcg catcgggctt cccatacaac cgatagattg tcgcacctga 1020 ttgcccgaca ttatcgcgcg cccatttata cccatataaa tcagcatcca tgttggaatt 1080 taatcgcggc ttcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt 1140 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat 1200 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga 1260 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgc tgtagtgcca 1320 tttaccccca ttcactgcca gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca 1380 gcgactcagg tgcctgatgg tcggagacaa aaggaatatt cagcgatttg cccgagcttg 1440 cgagggtgct acttaagcct ttagggtttt aaggtctgtt ttgtagagga gcaaacagcg 1500 tttgcgacat ccttttgtaa tactgcggaa ctgactaaag tagtgagtta tacacagggc 1560 tgggatctat tctttttatc tttttttatt ctttctttat tctataaatt ataaccactt 1620 gaatataaac aaaaaaaaca cacaaaggtc tagcggaatt tacagagggt ctagcagaat 1680 ttacaagttt tccagcaaag gtctagcaga atttacagat acccacaact caaaggaaaa 1740 ggactagtaa ttatcattga ctagcccatc tcaattggta tagtgattaa aatcacctag 1800 accaattgag atgtatgtct gaattagttg ttttcaaagc aaatgaacta gcgattagtc 1860 gctatgactt aacggagcat gaaaccaagc taattttatg ctgtgtggca ctactcaacc 1920 ccacgattga aaaccctaca aggaaagaac ggacggtatc gttcacttat aaccaatacg 1980 ttcagatgat gaacatcagt agggaaaatg cttatggtgt attagctaaa gcaaccagag 2040 agctgatgac gagaactgtg gaaatcagga atcctttggt taaaggcttt gagattttcc 2100 agtggacaaa ctatgccaag ttctcaagcg aaaaattaga attagttttt agtgaagaga 2160 tattgcctta tcttttccag ttaaaaaaat tcataaaata taatctggaa catgttaagt 2220 cttttgaaaa caaatactct atgaggattt atgagtggtt attaaaagaa ctaacacaaa 2280 agaaaactca caaggcaaat atagagatta gccttgatga atttaagttc atgttaatgc 2340 ttgaaaataa ctaccatgag tttaaaaggc ttaaccaatg ggttttgaaa ccaataagta 2400 aagatttaaa cacttacagc aatatgaaat tggtggttga taagcgaggc cgcccgactg 2460 atacgttgat tttccaagtt gaactagata gacaaatgga tctcgtaacc gaacttgaga 2520 acaaccagat aaaaatgaat ggtgacaaaa taccaacaac cattacatca gattcctacc 2580 tacataacgg actaagaaaa acactacacg atgctttaac tgcaaaaatt cagctcacca 2640 gttttgaggc aaaatttttg agtgacatgc aaagtaagta tgatctcaat ggttcgttct 2700 catggctcac gcaaaaacaa cgaaccacac tagagaacat actggctaaa tacggaagga 2760 tctgaggttc ttatggctct tgtatctatc agtgaagcat caagactaac aaacaaaagt 2820 agaacaactg ttcaccgtta catatcaaag ggaaaactgt ccataatgtg agttagctca 2880 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 2940 tgagcggata acaatttcac acaggaaaac at 2972 // ID AB019605; SV 1; circular; other DNA; STD; SYN; 2972 BP. XX AC AB019605; XX DT 04-DEC-1998 (Rel. 57, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 7) XX DE Cloning vector pTH18ks5 DNA, complete sequence. XX KW . XX OS Cloning vector pTH18ks5 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-2972 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; 97b21d7aad29e28aedac4d6730aae476. XX CC The copy number of pKF28ts5 is 1 per chromosome. CC Drug concentration for kanamycin selection is 20 microgramms per CC ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for alpha-complemetation selection . CC The multiple cloning sites are as in pKF18k (Gene, 1995; 167, CC 333-334). XX FH Key Location/Qualifiers FH FT source 1..2972 FT /organism="Cloning vector pTH18ks5" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84728" FT CDS 1..309 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF18k" FT /protein_id="BAA34747.1" FT /translation="MTMITPNSSSVPGDPLESTCRHASLLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF" FT CDS complement(310..1125) FT /codon_start=1 FT /transl_table=11 FT /gene="agp-5" FT /product="aminoglucoside phosphotransferase" FT /protein_id="BAA34748.1" FT /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT CDS 1815..2765 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="repAts5 in pHSG5" FT /protein_id="BAA34749.1" FT /translation="MSELVVFKANELTISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" FT variation 1851 FT /replace="g" XX SQ Sequence 2972 BP; 951 A; 634 C; 594 G; 793 T; 0 other; atgaccatga ttacgccgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc 60 aggcatgcaa gcttactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg cgatttattc 240 aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac 300 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 360 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 420 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 480 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg 540 agtgacgact gaatccggtg agaatggcaa gagcttatgc atttctttcc agacttgttc 600 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat 660 tcgtgactgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac 720 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga 780 atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag tggtgagtaa 840 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt 900 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg 960 tttcagaaac aactctggcg catcgggctt cccatacaac cgatagattg tcgcacctga 1020 ttgcccgaca ttatcgcgcg cccatttata cccatataaa tcagcatcca tgttggaatt 1080 taatcgcggc ttcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt 1140 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat 1200 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga 1260 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgc tgtagtgcca 1320 tttaccccca ttcactgcca gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca 1380 gcgactcagg tgcctgatgg tcggagacaa aaggaatatt cagcgatttg cccgagcttg 1440 cgagggtgct acttaagcct ttagggtttt aaggtctgtt ttgtagagga gcaaacagcg 1500 tttgcgacat ccttttgtaa tactgcggaa ctgactaaag tagtgagtta tacacagggc 1560 tgggatctat tctttttatc tttttttatt ctttctttat tctataaatt ataaccactt 1620 gaatataaac aaaaaaaaca cacaaaggtc tagcggaatt tacagagggt ctagcagaat 1680 ttacaagttt tccagcaaag gtctagcaga atttacagat acccacaact caaaggaaaa 1740 ggactagtaa ttatcattga ctagcccatc tcaattggta tagtgattaa aatcacctag 1800 accaattgag atgtatgtct gaattagttg ttttcaaagc aaatgaacta acgattagtc 1860 gctatgactt aacggagcat gaaaccaagc taattttatg ctgtgtggca ctactcaacc 1920 ccacgattga aaaccctaca aggaaagaac ggacggtatc gttcacttat aaccaatacg 1980 ctcagatgat gaacatcagt agggaaaatg cttatggtgt attagctaaa gcaaccagag 2040 agctgatgac gagaactgtg gaaatcagga atcctttggt taaaggcttt gagattttcc 2100 agtggacaaa ctatgccaag ttctcaagcg aaaaattaga attagttttt agtgaagaga 2160 tattgcctta tcttttccag ttaaaaaaat tcataaaata taatctggaa catgttaagt 2220 cttttgaaaa caaatactct atgaggattt atgagtggtt attaaaagaa ctaacacaaa 2280 agaaaactca caaggcaaat atagagatta gccttgatga atttaagttc atgttaatgc 2340 ttgaaaataa ctaccatgag tttaaaaggc ttaaccaatg ggttttgaaa ccaataagta 2400 aagatttaaa cacttacagc aatatgaaat tggtggttga taagcgaggc cgcccgactg 2460 atacgttgat tttccaagtt gaactagata gacaaatgga tctcgtaacc gaacttgaga 2520 acaaccagat aaaaatgaat ggtgacaaaa taccaacaac cattacatca gattcctacc 2580 tacataacgg actaagaaaa acactacacg atgctttaac tgcaaaaatt cagctcacca 2640 gttttgaggc aaaatttttg agtgacatgc aaagtaagta tgatctcaat ggttcgttct 2700 catggctcac gcaaaaacaa cgaaccacac tagagaacat actggctaaa tacggaagga 2760 tctgaggttc ttatggctct tgtatctatc agtgaagcat caagactaac aaacaaaagt 2820 agaacaactg ttcaccgtta catatcaaag ggaaaactgt ccataatgtg agttagctca 2880 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 2940 tgagcggata acaatttcac acaggaaaac at 2972 // ID AB019606; SV 1; circular; other DNA; STD; SYN; 2972 BP. XX AC AB019606; XX DT 04-DEC-1998 (Rel. 57, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 6) XX DE Cloning vector pTH19kr DNA, complete sequence. XX KW . XX OS Cloning vector pTH19kr OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-2972 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; dd898f8ad027ff944aa6cdde67a65dad. XX CC The copy number of pKF29wt is 5 per chromosome. CC Drug concentration for kanamycin selection is 20 microgramms per CC ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for alpha-complemetation selection . CC The multiple cloning sites are as in pKF19k (Gene, 1995; 167, CC 333-334). XX FH Key Location/Qualifiers FH FT source 1..2972 FT /organism="Cloning vector pTH19kr" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84729" FT CDS 1..309 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF18k" FT /protein_id="BAA34750.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF" FT CDS complement(310..1125) FT /codon_start=1 FT /transl_table=11 FT /gene="agp-5" FT /product="aminoglucoside phosphotransferase" FT /protein_id="BAA34751.1" FT /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT CDS 1815..2765 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="wild type repA in pSC101" FT /protein_id="BAA34752.1" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" XX SQ Sequence 2972 BP; 950 A; 635 C; 594 G; 793 T; 0 other; atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta 60 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg cgatttattc 240 aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac 300 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 360 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 420 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 480 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg 540 agtgacgact gaatccggtg agaatggcaa gagcttatgc atttctttcc agacttgttc 600 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat 660 tcgtgactgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac 720 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga 780 atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag tggtgagtaa 840 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt 900 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg 960 tttcagaaac aactctggcg catcgggctt cccatacaac cgatagattg tcgcacctga 1020 ttgcccgaca ttatcgcgcg cccatttata cccatataaa tcagcatcca tgttggaatt 1080 taatcgcggc ttcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt 1140 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat 1200 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga 1260 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgc tgtagtgcca 1320 tttaccccca ttcactgcca gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca 1380 gcgactcagg tgcctgatgg tcggagacaa aaggaatatt cagcgatttg cccgagcttg 1440 cgagggtgct acttaagcct ttagggtttt aaggtctgtt ttgtagagga gcaaacagcg 1500 tttgcgacat ccttttgtaa tactgcggaa ctgactaaag tagtgagtta tacacagggc 1560 tgggatctat tctttttatc tttttttatt ctttctttat tctataaatt ataaccactt 1620 gaatataaac aaaaaaaaca cacaaaggtc tagcggaatt tacagagggt ctagcagaat 1680 ttacaagttt tccagcaaag gtctagcaga atttacagat acccacaact caaaggaaaa 1740 ggactagtaa ttatcattga ctagcccatc tcaattggta tagtgattaa aatcacctag 1800 accaattgag atgtatgtct gaattagttg ttttcaaagc aaatgaacta gcgattagtc 1860 gctatgactt aacggagcat gaaaccaagc taattttatg ctgtgtggca ctactcaacc 1920 ccacgattga aaaccctaca aggaaagaac ggacggtatc gttcacttat aaccaatacg 1980 ctcagatgat gaacatcagt agggaaaatg cttatggtgt attagctaaa gcaaccagag 2040 agctgatgac gagaactgtg gaaatcagga atcctttggt taaaggcttt gagattttcc 2100 agtggacaaa ctatgccaag ttctcaagcg aaaaattaga attagttttt agtgaagaga 2160 tattgcctta tcttttccag ttaaaaaaat tcataaaata taatctggaa catgttaagt 2220 cttttgaaaa caaatactct atgaggattt atgagtggtt attaaaagaa ctaacacaaa 2280 agaaaactca caaggcaaat atagagatta gccttgatga atttaagttc atgttaatgc 2340 ttgaaaataa ctaccatgag tttaaaaggc ttaaccaatg ggttttgaaa ccaataagta 2400 aagatttaaa cacttacagc aatatgaaat tggtggttga taagcgaggc cgcccgactg 2460 atacgttgat tttccaagtt gaactagata gacaaatgga tctcgtaacc gaacttgaga 2520 acaaccagat aaaaatgaat ggtgacaaaa taccaacaac cattacatca gattcctacc 2580 tacataacgg actaagaaaa acactacacg atgctttaac tgcaaaaatt cagctcacca 2640 gttttgaggc aaaatttttg agtgacatgc aaagtaagta tgatctcaat ggttcgttct 2700 catggctcac gcaaaaacaa cgaaccacac tagagaacat actggctaaa tacggaagga 2760 tctgaggttc ttatggctct tgtatctatc agtgaagcat caagactaac aaacaaaagt 2820 agaacaactg ttcaccgtta catatcaaag ggaaaactgt ccataatgtg agttagctca 2880 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 2940 tgagcggata acaatttcac acaggaaaac at 2972 // ID AB019607; SV 1; circular; other DNA; STD; SYN; 2972 BP. XX AC AB019607; XX DT 04-DEC-1998 (Rel. 57, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 8) XX DE Cloning vector pTH19ks1 DNA, complete sequence. XX KW . XX OS Cloning vector pTH19ks1 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-2972 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; cc3989417bc9e959ccaa7645998d269c. XX CC The copy number of pKF29ts1 is 14 per chromosome. CC Drug concentration for kanamycin selection is 20 microgramms per CC ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for alpha-complemetation selection . CC The multiple cloning sites are as in pKF19k (Gene, 1995; 167, CC 333-334). XX FH Key Location/Qualifiers FH FT source 1..2972 FT /organism="Cloning vector pTH19ks1" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84730" FT CDS 1..309 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF18k" FT /protein_id="BAA34753.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF" FT CDS complement(310..1125) FT /codon_start=1 FT /transl_table=11 FT /gene="agp-5" FT /product="aminoglucoside phosphotransferase" FT /protein_id="BAA34754.1" FT /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT CDS 1815..2765 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="repAts1 in pHSG1" FT /protein_id="BAA34755.1" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" FT variation 1981 FT /replace="c" XX SQ Sequence 2972 BP; 950 A; 634 C; 594 G; 794 T; 0 other; atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta 60 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg cgatttattc 240 aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac 300 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 360 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 420 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 480 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg 540 agtgacgact gaatccggtg agaatggcaa gagcttatgc atttctttcc agacttgttc 600 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat 660 tcgtgactgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac 720 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga 780 atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag tggtgagtaa 840 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt 900 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg 960 tttcagaaac aactctggcg catcgggctt cccatacaac cgatagattg tcgcacctga 1020 ttgcccgaca ttatcgcgcg cccatttata cccatataaa tcagcatcca tgttggaatt 1080 taatcgcggc ttcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt 1140 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat 1200 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga 1260 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgc tgtagtgcca 1320 tttaccccca ttcactgcca gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca 1380 gcgactcagg tgcctgatgg tcggagacaa aaggaatatt cagcgatttg cccgagcttg 1440 cgagggtgct acttaagcct ttagggtttt aaggtctgtt ttgtagagga gcaaacagcg 1500 tttgcgacat ccttttgtaa tactgcggaa ctgactaaag tagtgagtta tacacagggc 1560 tgggatctat tctttttatc tttttttatt ctttctttat tctataaatt ataaccactt 1620 gaatataaac aaaaaaaaca cacaaaggtc tagcggaatt tacagagggt ctagcagaat 1680 ttacaagttt tccagcaaag gtctagcaga atttacagat acccacaact caaaggaaaa 1740 ggactagtaa ttatcattga ctagcccatc tcaattggta tagtgattaa aatcacctag 1800 accaattgag atgtatgtct gaattagttg ttttcaaagc aaatgaacta gcgattagtc 1860 gctatgactt aacggagcat gaaaccaagc taattttatg ctgtgtggca ctactcaacc 1920 ccacgattga aaaccctaca aggaaagaac ggacggtatc gttcacttat aaccaatacg 1980 ttcagatgat gaacatcagt agggaaaatg cttatggtgt attagctaaa gcaaccagag 2040 agctgatgac gagaactgtg gaaatcagga atcctttggt taaaggcttt gagattttcc 2100 agtggacaaa ctatgccaag ttctcaagcg aaaaattaga attagttttt agtgaagaga 2160 tattgcctta tcttttccag ttaaaaaaat tcataaaata taatctggaa catgttaagt 2220 cttttgaaaa caaatactct atgaggattt atgagtggtt attaaaagaa ctaacacaaa 2280 agaaaactca caaggcaaat atagagatta gccttgatga atttaagttc atgttaatgc 2340 ttgaaaataa ctaccatgag tttaaaaggc ttaaccaatg ggttttgaaa ccaataagta 2400 aagatttaaa cacttacagc aatatgaaat tggtggttga taagcgaggc cgcccgactg 2460 atacgttgat tttccaagtt gaactagata gacaaatgga tctcgtaacc gaacttgaga 2520 acaaccagat aaaaatgaat ggtgacaaaa taccaacaac cattacatca gattcctacc 2580 tacataacgg actaagaaaa acactacacg atgctttaac tgcaaaaatt cagctcacca 2640 gttttgaggc aaaatttttg agtgacatgc aaagtaagta tgatctcaat ggttcgttct 2700 catggctcac gcaaaaacaa cgaaccacac tagagaacat actggctaaa tacggaagga 2760 tctgaggttc ttatggctct tgtatctatc agtgaagcat caagactaac aaacaaaagt 2820 agaacaactg ttcaccgtta catatcaaag ggaaaactgt ccataatgtg agttagctca 2880 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 2940 tgagcggata acaatttcac acaggaaaac at 2972 // ID AB019608; SV 1; circular; other DNA; STD; SYN; 2972 BP. XX AC AB019608; XX DT 04-DEC-1998 (Rel. 57, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 7) XX DE Cloning vector pTH19ks5 DNA, complete sequence. XX KW . XX OS Cloning vector pTH19ks5 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-2972 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; b9323f1faf461ee2f0e8244c94ec8f32. XX CC The copy number of pKF29ts5 is 1 per chromosome. CC Drug concentration for kanamycin selection is 20 microgramms per CC ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for alpha-complemetation selection . CC The multiple cloning sites are as in pKF19k (Gene, 1995; 167, CC 333-334). XX FH Key Location/Qualifiers FH FT source 1..2972 FT /organism="Cloning vector pTH19ks5" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84731" FT CDS 1..309 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF18k" FT /protein_id="BAA34756.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF" FT CDS complement(310..1125) FT /codon_start=1 FT /transl_table=11 FT /gene="agp-5" FT /product="aminoglucoside phosphotransferase" FT /protein_id="BAA34757.1" FT /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT CDS 1815..2765 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="repAts5 in pHSG5" FT /protein_id="BAA34758.1" FT /translation="MSELVVFKANELTISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" FT variation 1851 FT /replace="g" XX SQ Sequence 2972 BP; 951 A; 635 C; 593 G; 793 T; 0 other; atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta 60 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg cgatttattc 240 aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca accaattaac 300 caattctgat tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg 360 attatcaata ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag 420 gcagttccat aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc 480 aatacaacct attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg 540 agtgacgact gaatccggtg agaatggcaa gagcttatgc atttctttcc agacttgttc 600 aacaggccag ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat 660 tcgtgactgc gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac 720 aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga 780 atcaggatat tcttctaata cctggaatgc tgtttttccg gggatcgcag tggtgagtaa 840 ccatgcatca tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt 900 cagccagttt agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg 960 tttcagaaac aactctggcg catcgggctt cccatacaac cgatagattg tcgcacctga 1020 ttgcccgaca ttatcgcgcg cccatttata cccatataaa tcagcatcca tgttggaatt 1080 taatcgcggc ttcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt 1140 actgtttatg taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat 1200 gtaacatcag agattttgag acacaacgtg gctttgttga ataaatcgaa cttttgctga 1260 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgc tgtagtgcca 1320 tttaccccca ttcactgcca gagccgtgag cgcagcgaac tgaatgtcac gaaaaagaca 1380 gcgactcagg tgcctgatgg tcggagacaa aaggaatatt cagcgatttg cccgagcttg 1440 cgagggtgct acttaagcct ttagggtttt aaggtctgtt ttgtagagga gcaaacagcg 1500 tttgcgacat ccttttgtaa tactgcggaa ctgactaaag tagtgagtta tacacagggc 1560 tgggatctat tctttttatc tttttttatt ctttctttat tctataaatt ataaccactt 1620 gaatataaac aaaaaaaaca cacaaaggtc tagcggaatt tacagagggt ctagcagaat 1680 ttacaagttt tccagcaaag gtctagcaga atttacagat acccacaact caaaggaaaa 1740 ggactagtaa ttatcattga ctagcccatc tcaattggta tagtgattaa aatcacctag 1800 accaattgag atgtatgtct gaattagttg ttttcaaagc aaatgaacta acgattagtc 1860 gctatgactt aacggagcat gaaaccaagc taattttatg ctgtgtggca ctactcaacc 1920 ccacgattga aaaccctaca aggaaagaac ggacggtatc gttcacttat aaccaatacg 1980 ctcagatgat gaacatcagt agggaaaatg cttatggtgt attagctaaa gcaaccagag 2040 agctgatgac gagaactgtg gaaatcagga atcctttggt taaaggcttt gagattttcc 2100 agtggacaaa ctatgccaag ttctcaagcg aaaaattaga attagttttt agtgaagaga 2160 tattgcctta tcttttccag ttaaaaaaat tcataaaata taatctggaa catgttaagt 2220 cttttgaaaa caaatactct atgaggattt atgagtggtt attaaaagaa ctaacacaaa 2280 agaaaactca caaggcaaat atagagatta gccttgatga atttaagttc atgttaatgc 2340 ttgaaaataa ctaccatgag tttaaaaggc ttaaccaatg ggttttgaaa ccaataagta 2400 aagatttaaa cacttacagc aatatgaaat tggtggttga taagcgaggc cgcccgactg 2460 atacgttgat tttccaagtt gaactagata gacaaatgga tctcgtaacc gaacttgaga 2520 acaaccagat aaaaatgaat ggtgacaaaa taccaacaac cattacatca gattcctacc 2580 tacataacgg actaagaaaa acactacacg atgctttaac tgcaaaaatt cagctcacca 2640 gttttgaggc aaaatttttg agtgacatgc aaagtaagta tgatctcaat ggttcgttct 2700 catggctcac gcaaaaacaa cgaaccacac tagagaacat actggctaaa tacggaagga 2760 tctgaggttc ttatggctct tgtatctatc agtgaagcat caagactaac aaacaaaagt 2820 agaacaactg ttcaccgtta catatcaaag ggaaaactgt ccataatgtg agttagctca 2880 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg 2940 tgagcggata acaatttcac acaggaaaac at 2972 // ID AB019609; SV 2; circular; other DNA; STD; SYN; 3064 BP. XX AC AB019609; XX DT 14-DEC-1998 (Rel. 58, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 6) XX DE Cloning vector pTH18cr DNA, complete sequence. XX KW . XX OS Cloning vector pTH18cr OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3064 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; 938ba2ab633e8a9068637f3e89347811. XX CC The copy number of pKF38wt is 5 per chromosome. Drug concentration CC for chloramphenicol selection should be 10 to 15 microgramms per CC ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for alpha-complemetation selection . CC The multiple cloning sites are as in pKF18c (Gene, 1994; 152, CC 271-275). XX FH Key Location/Qualifiers FH FT source 1..3064 FT /organism="Cloning vector pTH18cr" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84732" FT CDS 1..237 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF18c" FT /protein_id="BAA34759.1" FT /translation="MTMITPNSSSVPGDPLESTCRHASLLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR" FT CDS complement(534..1193) FT /codon_start=1 FT /transl_table=11 FT /gene="cat-1" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BAA34760.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS 1907..2857 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="wild type repA in pSC101" FT /protein_id="BAA34761.1" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" XX SQ Sequence 3064 BP; 960 A; 657 C; 633 G; 814 T; 0 other; atgaccatga ttacgccgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc 60 aggcatgcaa gcttactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctaaccg 240 tttttatcag gctctgggag gcagaataaa tgatcatatc gtcaattatt acctccacgg 300 ggagagcctg agcaaactgg cctcaggcat ttgagaagca cacggtcaca ctgcttccgg 360 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg 420 aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact 480 tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc 540 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag 600 ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 660 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 720 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 780 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 840 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 900 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 960 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 1020 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 1080 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 1140 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 1200 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca 1260 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg 1320 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct 1380 tccgtcacag gtatttattc gctgtagtgc catttacccc cattcactgc cagagccgtg 1440 agcgcagcga actgaatgtc acgaaaaaga cagcgactca ggtgcctgat ggtcggagac 1500 aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg ctacttaagc ctttagggtt 1560 ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac atccttttgt aatactgcgg 1620 aactgactaa agtagtgagt tatacacagg gctgggatct attcttttta tcttttttta 1680 ttctttcttt attctataaa ttataaccac ttgaatataa acaaaaaaaa cacacaaagg 1740 tctagcggaa tttacagagg gtctagcaga atttacaagt tttccagcaa aggtctagca 1800 gaatttacag atacccacaa ctcaaaggaa aaggactagt aattatcatt gactagccca 1860 tctcaattgg tatagtgatt aaaatcacct agaccaattg agatgtatgt ctgaattagt 1920 tgttttcaaa gcaaatgaac tagcgattag tcgctatgac ttaacggagc atgaaaccaa 1980 gctaatttta tgctgtgtgg cactactcaa ccccacgatt gaaaacccta caaggaaaga 2040 acggacggta tcgttcactt ataaccaata cgctcagatg atgaacatca gtagggaaaa 2100 tgcttatggt gtattagcta aagcaaccag agagctgatg acgagaactg tggaaatcag 2160 gaatcctttg gttaaaggct ttgagatttt ccagtggaca aactatgcca agttctcaag 2220 cgaaaaatta gaattagttt ttagtgaaga gatattgcct tatcttttcc agttaaaaaa 2280 attcataaaa tataatctgg aacatgttaa gtcttttgaa aacaaatact ctatgaggat 2340 ttatgagtgg ttattaaaag aactaacaca aaagaaaact cacaaggcaa atatagagat 2400 tagccttgat gaatttaagt tcatgttaat gcttgaaaat aactaccatg agtttaaaag 2460 gcttaaccaa tgggttttga aaccaataag taaagattta aacacttaca gcaatatgaa 2520 attggtggtt gataagcgag gccgcccgac tgatacgttg attttccaag ttgaactaga 2580 tagacaaatg gatctcgtaa ccgaacttga gaacaaccag ataaaaatga atggtgacaa 2640 aataccaaca accattacat cagattccta cctacataac ggactaagaa aaacactaca 2700 cgatgcttta actgcaaaaa ttcagctcac cagttttgag gcaaaatttt tgagtgacat 2760 gcaaagtaag tatgatctca atggttcgtt ctcatggctc acgcaaaaac aacgaaccac 2820 actagagaac atactggcta aatacggaag gatctgaggt tcttatggct cttgtatcta 2880 tcagtgaagc atcaagacta acaaacaaaa gtagaacaac tgttcaccgt tacatatcaa 2940 agggaaaact gtccataatg tgagttagct cactcattag gcaccccagg ctttacactt 3000 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 3060 acat 3064 // ID AB019610; SV 2; circular; other DNA; STD; SYN; 3064 BP. XX AC AB019610; XX DT 04-DEC-1998 (Rel. 57, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 8) XX DE Cloning vector pTH18cs1 DNA, complete sequence. XX KW . XX OS Cloning vector pTH18cs1 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3064 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; baf4c73b3e64c8a7b2b51a1b804c7d9b. XX CC The copy number of pKF38ts1 is 14 per chromosome. CC Drug concentration for chloramphenicol selection should be 10 to CC 15 microgramms per ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for alpha-complemetation selection . CC The multiple cloning sites are as in pKF18c (Gene, 1994; 152, CC 271-275). XX FH Key Location/Qualifiers FH FT source 1..3064 FT /organism="Cloning vector pTH18cs1" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84733" FT CDS 1..237 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF18c" FT /protein_id="BAA34762.1" FT /translation="MTMITPNSSSVPGDPLESTCRHASLLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR" FT CDS complement(534..1193) FT /codon_start=1 FT /transl_table=11 FT /gene="cat-1" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BAA34763.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS 1907..2857 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="repAts1 in pHSG1" FT /protein_id="BAA34764.1" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" FT variation 2073 FT /replace="c" XX SQ Sequence 3064 BP; 960 A; 656 C; 633 G; 815 T; 0 other; atgaccatga ttacgccgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc 60 aggcatgcaa gcttactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctaaccg 240 tttttatcag gctctgggag gcagaataaa tgatcatatc gtcaattatt acctccacgg 300 ggagagcctg agcaaactgg cctcaggcat ttgagaagca cacggtcaca ctgcttccgg 360 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg 420 aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact 480 tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc 540 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag 600 ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 660 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 720 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 780 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 840 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 900 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 960 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 1020 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 1080 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 1140 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 1200 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca 1260 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg 1320 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct 1380 tccgtcacag gtatttattc gctgtagtgc catttacccc cattcactgc cagagccgtg 1440 agcgcagcga actgaatgtc acgaaaaaga cagcgactca ggtgcctgat ggtcggagac 1500 aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg ctacttaagc ctttagggtt 1560 ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac atccttttgt aatactgcgg 1620 aactgactaa agtagtgagt tatacacagg gctgggatct attcttttta tcttttttta 1680 ttctttcttt attctataaa ttataaccac ttgaatataa acaaaaaaaa cacacaaagg 1740 tctagcggaa tttacagagg gtctagcaga atttacaagt tttccagcaa aggtctagca 1800 gaatttacag atacccacaa ctcaaaggaa aaggactagt aattatcatt gactagccca 1860 tctcaattgg tatagtgatt aaaatcacct agaccaattg agatgtatgt ctgaattagt 1920 tgttttcaaa gcaaatgaac tagcgattag tcgctatgac ttaacggagc atgaaaccaa 1980 gctaatttta tgctgtgtgg cactactcaa ccccacgatt gaaaacccta caaggaaaga 2040 acggacggta tcgttcactt ataaccaata cgttcagatg atgaacatca gtagggaaaa 2100 tgcttatggt gtattagcta aagcaaccag agagctgatg acgagaactg tggaaatcag 2160 gaatcctttg gttaaaggct ttgagatttt ccagtggaca aactatgcca agttctcaag 2220 cgaaaaatta gaattagttt ttagtgaaga gatattgcct tatcttttcc agttaaaaaa 2280 attcataaaa tataatctgg aacatgttaa gtcttttgaa aacaaatact ctatgaggat 2340 ttatgagtgg ttattaaaag aactaacaca aaagaaaact cacaaggcaa atatagagat 2400 tagccttgat gaatttaagt tcatgttaat gcttgaaaat aactaccatg agtttaaaag 2460 gcttaaccaa tgggttttga aaccaataag taaagattta aacacttaca gcaatatgaa 2520 attggtggtt gataagcgag gccgcccgac tgatacgttg attttccaag ttgaactaga 2580 tagacaaatg gatctcgtaa ccgaacttga gaacaaccag ataaaaatga atggtgacaa 2640 aataccaaca accattacat cagattccta cctacataac ggactaagaa aaacactaca 2700 cgatgcttta actgcaaaaa ttcagctcac cagttttgag gcaaaatttt tgagtgacat 2760 gcaaagtaag tatgatctca atggttcgtt ctcatggctc acgcaaaaac aacgaaccac 2820 actagagaac atactggcta aatacggaag gatctgaggt tcttatggct cttgtatcta 2880 tcagtgaagc atcaagacta acaaacaaaa gtagaacaac tgttcaccgt tacatatcaa 2940 agggaaaact gtccataatg tgagttagct cactcattag gcaccccagg ctttacactt 3000 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 3060 acat 3064 // ID AB019611; SV 2; circular; other DNA; STD; SYN; 3064 BP. XX AC AB019611; XX DT 04-DEC-1998 (Rel. 57, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 7) XX DE Cloning vector pTH18cs5 DNA, complete sequence. XX KW . XX OS Cloning vector pTH18cs5 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3064 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; 6e4923dfaed18ea855a73c06ee51c69a. XX CC The copy number of pKF38ts5 is 1 per chromosome. CC Drug concentration for chloramphenicol selection should be 8 to 10 CC microgramms per ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for alpha-complemetation selection . CC The multiple cloning sites are as in pKF18c (Gene, 1994; 152, CC 271-275). XX FH Key Location/Qualifiers FH FT source 1..3064 FT /organism="Cloning vector pTH18cs5" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84734" FT CDS 1..237 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF18c" FT /protein_id="BAA34765.1" FT /translation="MTMITPNSSSVPGDPLESTCRHASLLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR" FT CDS complement(534..1193) FT /codon_start=1 FT /transl_table=11 FT /gene="cat-1" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BAA34766.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS 1907..2857 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="repAts5 in pHSG5" FT /protein_id="BAA34767.1" FT /translation="MSELVVFKANELTISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" FT variation 1943 FT /replace="g" XX SQ Sequence 3064 BP; 961 A; 657 C; 632 G; 814 T; 0 other; atgaccatga ttacgccgaa ttcgagctcg gtacccgggg atcctctaga gtcgacctgc 60 aggcatgcaa gcttactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctaaccg 240 tttttatcag gctctgggag gcagaataaa tgatcatatc gtcaattatt acctccacgg 300 ggagagcctg agcaaactgg cctcaggcat ttgagaagca cacggtcaca ctgcttccgg 360 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg 420 aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact 480 tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc 540 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag 600 ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 660 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 720 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 780 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 840 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 900 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 960 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 1020 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 1080 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 1140 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 1200 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca 1260 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg 1320 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct 1380 tccgtcacag gtatttattc gctgtagtgc catttacccc cattcactgc cagagccgtg 1440 agcgcagcga actgaatgtc acgaaaaaga cagcgactca ggtgcctgat ggtcggagac 1500 aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg ctacttaagc ctttagggtt 1560 ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac atccttttgt aatactgcgg 1620 aactgactaa agtagtgagt tatacacagg gctgggatct attcttttta tcttttttta 1680 ttctttcttt attctataaa ttataaccac ttgaatataa acaaaaaaaa cacacaaagg 1740 tctagcggaa tttacagagg gtctagcaga atttacaagt tttccagcaa aggtctagca 1800 gaatttacag atacccacaa ctcaaaggaa aaggactagt aattatcatt gactagccca 1860 tctcaattgg tatagtgatt aaaatcacct agaccaattg agatgtatgt ctgaattagt 1920 tgttttcaaa gcaaatgaac taacgattag tcgctatgac ttaacggagc atgaaaccaa 1980 gctaatttta tgctgtgtgg cactactcaa ccccacgatt gaaaacccta caaggaaaga 2040 acggacggta tcgttcactt ataaccaata cgctcagatg atgaacatca gtagggaaaa 2100 tgcttatggt gtattagcta aagcaaccag agagctgatg acgagaactg tggaaatcag 2160 gaatcctttg gttaaaggct ttgagatttt ccagtggaca aactatgcca agttctcaag 2220 cgaaaaatta gaattagttt ttagtgaaga gatattgcct tatcttttcc agttaaaaaa 2280 attcataaaa tataatctgg aacatgttaa gtcttttgaa aacaaatact ctatgaggat 2340 ttatgagtgg ttattaaaag aactaacaca aaagaaaact cacaaggcaa atatagagat 2400 tagccttgat gaatttaagt tcatgttaat gcttgaaaat aactaccatg agtttaaaag 2460 gcttaaccaa tgggttttga aaccaataag taaagattta aacacttaca gcaatatgaa 2520 attggtggtt gataagcgag gccgcccgac tgatacgttg attttccaag ttgaactaga 2580 tagacaaatg gatctcgtaa ccgaacttga gaacaaccag ataaaaatga atggtgacaa 2640 aataccaaca accattacat cagattccta cctacataac ggactaagaa aaacactaca 2700 cgatgcttta actgcaaaaa ttcagctcac cagttttgag gcaaaatttt tgagtgacat 2760 gcaaagtaag tatgatctca atggttcgtt ctcatggctc acgcaaaaac aacgaaccac 2820 actagagaac atactggcta aatacggaag gatctgaggt tcttatggct cttgtatcta 2880 tcagtgaagc atcaagacta acaaacaaaa gtagaacaac tgttcaccgt tacatatcaa 2940 agggaaaact gtccataatg tgagttagct cactcattag gcaccccagg ctttacactt 3000 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 3060 acat 3064 // ID AB019612; SV 2; circular; other DNA; STD; SYN; 3064 BP. XX AC AB019612; XX DT 04-DEC-1998 (Rel. 57, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 6) XX DE Cloning vector pTH19cr DNA, complete sequence. XX KW . XX OS Cloning vector pTH19cr OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3064 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; e9ef285c313c3d90c2dd7dbaca84fb57. XX CC The copy number of pKF39wt is 5 per chromosome. CC Drug concentration for chloramphenicol selection should be 8 to CC 10 microgramms per ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for alpha-complemetation selection. CC The multiple cloning sites are as in pKF19c (Gene, 1994; 152, CC 271-275). XX FH Key Location/Qualifiers FH FT source 1..3064 FT /organism="Cloning vector pTH19cr" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84735" FT CDS 1..237 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF19c" FT /protein_id="BAA34768.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR" FT CDS complement(534..1193) FT /codon_start=1 FT /transl_table=11 FT /gene="cat-1" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BAA34769.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS 1907..2857 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="wild type repA in pSC101" FT /protein_id="BAA34770.1" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" XX SQ Sequence 3064 BP; 960 A; 658 C; 632 G; 814 T; 0 other; atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta 60 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctaaccg 240 tttttatcag gctctgggag gcagaataaa tgatcatatc gtcaattatt acctccacgg 300 ggagagcctg agcaaactgg cctcaggcat ttgagaagca cacggtcaca ctgcttccgg 360 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg 420 aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact 480 tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc 540 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag 600 ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 660 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 720 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 780 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 840 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 900 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 960 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 1020 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 1080 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 1140 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 1200 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca 1260 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg 1320 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct 1380 tccgtcacag gtatttattc gctgtagtgc catttacccc cattcactgc cagagccgtg 1440 agcgcagcga actgaatgtc acgaaaaaga cagcgactca ggtgcctgat ggtcggagac 1500 aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg ctacttaagc ctttagggtt 1560 ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac atccttttgt aatactgcgg 1620 aactgactaa agtagtgagt tatacacagg gctgggatct attcttttta tcttttttta 1680 ttctttcttt attctataaa ttataaccac ttgaatataa acaaaaaaaa cacacaaagg 1740 tctagcggaa tttacagagg gtctagcaga atttacaagt tttccagcaa aggtctagca 1800 gaatttacag atacccacaa ctcaaaggaa aaggactagt aattatcatt gactagccca 1860 tctcaattgg tatagtgatt aaaatcacct agaccaattg agatgtatgt ctgaattagt 1920 tgttttcaaa gcaaatgaac tagcgattag tcgctatgac ttaacggagc atgaaaccaa 1980 gctaatttta tgctgtgtgg cactactcaa ccccacgatt gaaaacccta caaggaaaga 2040 acggacggta tcgttcactt ataaccaata cgctcagatg atgaacatca gtagggaaaa 2100 tgcttatggt gtattagcta aagcaaccag agagctgatg acgagaactg tggaaatcag 2160 gaatcctttg gttaaaggct ttgagatttt ccagtggaca aactatgcca agttctcaag 2220 cgaaaaatta gaattagttt ttagtgaaga gatattgcct tatcttttcc agttaaaaaa 2280 attcataaaa tataatctgg aacatgttaa gtcttttgaa aacaaatact ctatgaggat 2340 ttatgagtgg ttattaaaag aactaacaca aaagaaaact cacaaggcaa atatagagat 2400 tagccttgat gaatttaagt tcatgttaat gcttgaaaat aactaccatg agtttaaaag 2460 gcttaaccaa tgggttttga aaccaataag taaagattta aacacttaca gcaatatgaa 2520 attggtggtt gataagcgag gccgcccgac tgatacgttg attttccaag ttgaactaga 2580 tagacaaatg gatctcgtaa ccgaacttga gaacaaccag ataaaaatga atggtgacaa 2640 aataccaaca accattacat cagattccta cctacataac ggactaagaa aaacactaca 2700 cgatgcttta actgcaaaaa ttcagctcac cagttttgag gcaaaatttt tgagtgacat 2760 gcaaagtaag tatgatctca atggttcgtt ctcatggctc acgcaaaaac aacgaaccac 2820 actagagaac atactggcta aatacggaag gatctgaggt tcttatggct cttgtatcta 2880 tcagtgaagc atcaagacta acaaacaaaa gtagaacaac tgttcaccgt tacatatcaa 2940 agggaaaact gtccataatg tgagttagct cactcattag gcaccccagg ctttacactt 3000 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 3060 acat 3064 // ID AB019613; SV 2; circular; other DNA; STD; SYN; 3064 BP. XX AC AB019613; XX DT 04-DEC-1998 (Rel. 57, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 8) XX DE Cloning vector pTH19cs1 DNA, complete sequence. XX KW . XX OS Cloning vector pTH19cs1 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3064 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; 841078b933c477c4f8bee64056042e7c. XX CC The copy number of pKF39ts1 is 14 per chromosome. CC Drug concentration for chloramphenicol selection should be 10 to CC 15 microgramms per ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for alpha-complemetation selection. CC The multiple cloning sites are as in pKF19c (Gene, 1994; 152, CC 271-275). XX FH Key Location/Qualifiers FH FT source 1..3064 FT /organism="Cloning vector pTH19cs1" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84736" FT CDS 1..237 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF19c" FT /protein_id="BAA34771.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR" FT CDS complement(534..1193) FT /codon_start=1 FT /transl_table=11 FT /gene="cat-1" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BAA34772.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS 1907..2857 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="repAts1 in pHSG1" FT /protein_id="BAA34773.1" FT /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" FT variation 2073 FT /replace="c" XX SQ Sequence 3064 BP; 960 A; 657 C; 632 G; 815 T; 0 other; atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta 60 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctaaccg 240 tttttatcag gctctgggag gcagaataaa tgatcatatc gtcaattatt acctccacgg 300 ggagagcctg agcaaactgg cctcaggcat ttgagaagca cacggtcaca ctgcttccgg 360 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg 420 aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact 480 tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc 540 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag 600 ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 660 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 720 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 780 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 840 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 900 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 960 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 1020 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 1080 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 1140 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 1200 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca 1260 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg 1320 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct 1380 tccgtcacag gtatttattc gctgtagtgc catttacccc cattcactgc cagagccgtg 1440 agcgcagcga actgaatgtc acgaaaaaga cagcgactca ggtgcctgat ggtcggagac 1500 aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg ctacttaagc ctttagggtt 1560 ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac atccttttgt aatactgcgg 1620 aactgactaa agtagtgagt tatacacagg gctgggatct attcttttta tcttttttta 1680 ttctttcttt attctataaa ttataaccac ttgaatataa acaaaaaaaa cacacaaagg 1740 tctagcggaa tttacagagg gtctagcaga atttacaagt tttccagcaa aggtctagca 1800 gaatttacag atacccacaa ctcaaaggaa aaggactagt aattatcatt gactagccca 1860 tctcaattgg tatagtgatt aaaatcacct agaccaattg agatgtatgt ctgaattagt 1920 tgttttcaaa gcaaatgaac tagcgattag tcgctatgac ttaacggagc atgaaaccaa 1980 gctaatttta tgctgtgtgg cactactcaa ccccacgatt gaaaacccta caaggaaaga 2040 acggacggta tcgttcactt ataaccaata cgttcagatg atgaacatca gtagggaaaa 2100 tgcttatggt gtattagcta aagcaaccag agagctgatg acgagaactg tggaaatcag 2160 gaatcctttg gttaaaggct ttgagatttt ccagtggaca aactatgcca agttctcaag 2220 cgaaaaatta gaattagttt ttagtgaaga gatattgcct tatcttttcc agttaaaaaa 2280 attcataaaa tataatctgg aacatgttaa gtcttttgaa aacaaatact ctatgaggat 2340 ttatgagtgg ttattaaaag aactaacaca aaagaaaact cacaaggcaa atatagagat 2400 tagccttgat gaatttaagt tcatgttaat gcttgaaaat aactaccatg agtttaaaag 2460 gcttaaccaa tgggttttga aaccaataag taaagattta aacacttaca gcaatatgaa 2520 attggtggtt gataagcgag gccgcccgac tgatacgttg attttccaag ttgaactaga 2580 tagacaaatg gatctcgtaa ccgaacttga gaacaaccag ataaaaatga atggtgacaa 2640 aataccaaca accattacat cagattccta cctacataac ggactaagaa aaacactaca 2700 cgatgcttta actgcaaaaa ttcagctcac cagttttgag gcaaaatttt tgagtgacat 2760 gcaaagtaag tatgatctca atggttcgtt ctcatggctc acgcaaaaac aacgaaccac 2820 actagagaac atactggcta aatacggaag gatctgaggt tcttatggct cttgtatcta 2880 tcagtgaagc atcaagacta acaaacaaaa gtagaacaac tgttcaccgt tacatatcaa 2940 agggaaaact gtccataatg tgagttagct cactcattag gcaccccagg ctttacactt 3000 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 3060 acat 3064 // ID AB019614; SV 2; circular; other DNA; STD; SYN; 3064 BP. XX AC AB019614; XX DT 04-DEC-1998 (Rel. 57, Created) DT 16-DEC-2008 (Rel. 98, Last updated, Version 7) XX DE Cloning vector pTH19cs5 DNA, complete sequence. XX KW . XX OS Cloning vector pTH19cs5 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3064 RA Hashimoto-Gotoh T.; RT ; RL Submitted (10-NOV-1998) to the INSDC. RL Contact:Tamotsu Hashimoto-Gotoh Res. Inst. for Geriatrics, Kyoto Pref. RL Univ. Med., Biochem. and Mol. Genet.; Kawaramachi-Hirokoji, Kajii-Cho 465, RL Kamigyo-ku, Kyoto, Kyoto 602-8566, Japan XX RN [2] RX DOI; 10.1016/S0378-1119(99)00434-5. RX PUBMED; 10607913. RA Hashimoto-Gotoh T., Yamaguchi M., Yasojima K., Tsujimura A., RA Wakabayashi Y., Watanabe Y.; RT "A set of temperature sensitive-replication/-segregation and temperature RT resistant plasmid vectors with different copy numbers and in an isogenic RT background (chloramphenicol, kanamycin, lacZ, repA, par, polA)"; RL Gene 241(1):185-191(2000). XX DR MD5; 4645a86a8a3e617642931ab7beaf09b7. XX CC The copy number of pKF39ts5 is 1 per chromosome. CC Drug concentration for chloramphenicol selection should be 8 to 10 CC microgramms per ml. CC TOP10 but neither DH5alpha nor JM109 is advisable as a host strain CC for alpha-complemetation selection. CC The multiple cloning sites are as in pKF19c (Gene, 1994; 152, CC 271-275). XX FH Key Location/Qualifiers FH FT source 1..3064 FT /organism="Cloning vector pTH19cs5" FT /host="Escherichia coli" FT /mol_type="other DNA" FT /note="plasmid vector" FT /db_xref="taxon:84737" FT CDS 1..237 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha-peptide" FT /note="MCS as those in pKF19c" FT /protein_id="BAA34774.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWR" FT CDS complement(534..1193) FT /codon_start=1 FT /transl_table=11 FT /gene="cat-1" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BAA34775.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS 1907..2857 FT /codon_start=1 FT /transl_table=11 FT /product="replication initiator" FT /note="repAts5 in pHSG5" FT /protein_id="BAA34776.1" FT /translation="MSELVVFKANELTISRYDLTEHETKLILCCVALLNPTIENPTRKE FT RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS FT SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI FT EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV FT ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF FT LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI" FT variation 1943 FT /replace="g" XX SQ Sequence 3064 BP; 961 A; 658 C; 631 G; 814 T; 0 other; atgaccatga ttacgccaag cttgcatgcc tgcaggtcga ctctagagga tccccgggta 60 ccgagctcga attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt 120 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag 180 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgctaaccg 240 tttttatcag gctctgggag gcagaataaa tgatcatatc gtcaattatt acctccacgg 300 ggagagcctg agcaaactgg cctcaggcat ttgagaagca cacggtcaca ctgcttccgg 360 tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga ccctgccctg 420 aaccgacgac cgggtcgaat ttgctttcga atttctgcca ttcatccgct tattatcact 480 tattcaggcg tagcaccagg cgtttaaggg caccaataac tgccttaaaa aaattacgcc 540 ccgccctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag 600 ccatcacaga cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc 660 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt 720 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata 780 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg 840 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt 900 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct 960 ttcattgcca tacgaaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag 1020 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc 1080 tgaacggtct ggttataggt acattgagca actgactgaa atgcctcaaa atgttcttta 1140 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct 1200 tccttagctc ctgaaaatct cgataactca aaaaatacgc ccggtagtga tcttatttca 1260 ttatggtgaa agttggaacc tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg 1320 gcccagggct tcccggtatc aacagggaca ccaggattta tttattctgc gaagtgatct 1380 tccgtcacag gtatttattc gctgtagtgc catttacccc cattcactgc cagagccgtg 1440 agcgcagcga actgaatgtc acgaaaaaga cagcgactca ggtgcctgat ggtcggagac 1500 aaaaggaata ttcagcgatt tgcccgagct tgcgagggtg ctacttaagc ctttagggtt 1560 ttaaggtctg ttttgtagag gagcaaacag cgtttgcgac atccttttgt aatactgcgg 1620 aactgactaa agtagtgagt tatacacagg gctgggatct attcttttta tcttttttta 1680 ttctttcttt attctataaa ttataaccac ttgaatataa acaaaaaaaa cacacaaagg 1740 tctagcggaa tttacagagg gtctagcaga atttacaagt tttccagcaa aggtctagca 1800 gaatttacag atacccacaa ctcaaaggaa aaggactagt aattatcatt gactagccca 1860 tctcaattgg tatagtgatt aaaatcacct agaccaattg agatgtatgt ctgaattagt 1920 tgttttcaaa gcaaatgaac taacgattag tcgctatgac ttaacggagc atgaaaccaa 1980 gctaatttta tgctgtgtgg cactactcaa ccccacgatt gaaaacccta caaggaaaga 2040 acggacggta tcgttcactt ataaccaata cgctcagatg atgaacatca gtagggaaaa 2100 tgcttatggt gtattagcta aagcaaccag agagctgatg acgagaactg tggaaatcag 2160 gaatcctttg gttaaaggct ttgagatttt ccagtggaca aactatgcca agttctcaag 2220 cgaaaaatta gaattagttt ttagtgaaga gatattgcct tatcttttcc agttaaaaaa 2280 attcataaaa tataatctgg aacatgttaa gtcttttgaa aacaaatact ctatgaggat 2340 ttatgagtgg ttattaaaag aactaacaca aaagaaaact cacaaggcaa atatagagat 2400 tagccttgat gaatttaagt tcatgttaat gcttgaaaat aactaccatg agtttaaaag 2460 gcttaaccaa tgggttttga aaccaataag taaagattta aacacttaca gcaatatgaa 2520 attggtggtt gataagcgag gccgcccgac tgatacgttg attttccaag ttgaactaga 2580 tagacaaatg gatctcgtaa ccgaacttga gaacaaccag ataaaaatga atggtgacaa 2640 aataccaaca accattacat cagattccta cctacataac ggactaagaa aaacactaca 2700 cgatgcttta actgcaaaaa ttcagctcac cagttttgag gcaaaatttt tgagtgacat 2760 gcaaagtaag tatgatctca atggttcgtt ctcatggctc acgcaaaaac aacgaaccac 2820 actagagaac atactggcta aatacggaag gatctgaggt tcttatggct cttgtatcta 2880 tcagtgaagc atcaagacta acaaacaaaa gtagaacaac tgttcaccgt tacatatcaa 2940 agggaaaact gtccataatg tgagttagct cactcattag gcaccccagg ctttacactt 3000 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa 3060 acat 3064 // ID AB020028; SV 2; circular; other DNA; STD; SYN; 22530 BP. XX AC AB020028; XX DT 15-JUN-1999 (Rel. 60, Created) DT 27-DEC-2006 (Rel. 90, Last updated, Version 4) XX DE Cloning vector pYLTAC7 DNA, complete sequence. XX KW . XX OS Cloning vector pYLTAC7 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-22530 RA Nakamura Y.; RT ; RL Submitted (17-NOV-1998) to the INSDC. RL Yasukazu Nakamura, Kazusa DNA Research Institute, The First Laboratory for RL Plant Gene Research; Yana 1532-3, Kisarazu, Chiba 292-0812, Japan RL (E-mail:ynakamu@kazusa.or.jp, URL:http://www.kazusa.jp/%7Eynakamu/, RL Tel:81-438-52-3935(ex.1236), Fax:81-438-52-3934) XX RN [2] RX DOI; 10.1073/pnas.96.11.6535. RX PUBMED; 10339623. RA Liu Y.G., Shirano Y., Fukaki H., Yanai Y., Tasaka M., Tabata S., RA Shibata D.; RT "Complementation of plant mutants with large genomic DNA fragments by a RT transformation-competent artificial chromosome vector accelerates RT positional cloning"; RL Proc. Natl. Acad. Sci. U.S.A. 96(11):6535-6540(1999). XX DR MD5; 1f4c2b16d58116a4a718aba07998ec94. DR EuropePMC; PMC26917; 10339623. XX FH Key Location/Qualifiers FH FT source 1..22530 FT /organism="Cloning vector pYLTAC7" FT /mol_type="other DNA" FT /note="feature information will be reported soon" FT /note="transformation-competent artificial chromosome (TAC) FT vector pYLTAC7" FT /db_xref="taxon:96766" XX SQ Sequence 22530 BP; 5729 A; 5541 C; 5698 G; 5562 T; 0 other; aagcttggat ccgcccgggc ctaattggcc ggcgcgccaa aaatttatta gagcaatata 60 gtcctagaat gtcaaaggta ccgatcatga tctagaattc ccgatctagt aacatagatg 120 acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt 180 aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt 240 acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa 300 gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga 360 aattcggggg atcgatcccg cgaactgtgg acgagaactg tgccaccaag cgtaaggccg 420 ttctctcgca tttgccttgc taggctcgcg cgagttgctg gctgaggcgt tctcgaaatc 480 agctcttgtt cggtcggcat ctactctatt cctttgccct cggacgagtg ctggggcgtc 540 ggtttccact atcggcgagt acttctacac agccatcggt ccagacggcc gcgcttctgc 600 gggcgatttg tgtacgcccg acagtcccgg ctccggatcg gacgattgcg tcgcatcgac 660 cctgcgccca agctgcatca tcgaaattgc cgtcaaccaa gctctgatag agttggtcaa 720 gaccaatgcg gagcatatac gcccggagcc gcggcgatcc tgcaagctcc ggatgcctcc 780 gctcgaagta gcgcgtctgc tgctccatac aagccaacca cggcctccag aagaagatgt 840 tggcgacctc gtattgggaa tccccgaaca tcgcctcgct ccagtcaatg accgctgtta 900 tgcggccatt gtccgtcagg acattgttgg agccgaaatc cgcgtgcacg aggtgccgga 960 cttcggggca gtcctcggcc caaagcatca gctcatcgag agcctgcgcg acggacgcac 1020 tgacggtgtc gtccatcaca gtttgccagt gatacacatg gggatcagca atcgcgcata 1080 tgaaatcacg ccatgtagtg tattgaccga ttccttgcgg tccgaatggg ccgaacccgc 1140 tcgtctggct aagatcggcc gcagcgatcg catccatggc ctccgcgacc ggctgcagaa 1200 cagcgggcag ttcggtttca ggcaggtctt gcaacgtgac accctgtgca cggcgggaga 1260 tgcaataggt caggctctcg ctgaatgccc caatgtcaag cacttccgga atcgggagcg 1320 cggccgatgc aaagtgccga taaacataac gatctttgta gaaaccatcg gcgcagctat 1380 ttacccgcag gacatatcca cgccctccta catcgaagct gaaagcacga gattcttcgc 1440 cctccgagag ctgcatcagg tcggagacgc tgtcgaactt ttcgatcaga aacttctcga 1500 cagacgtcgc ggtgagttca ggctttttca tatcttattg ccccccggga tcagatctgg 1560 attgagagtg aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg 1620 agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg 1680 caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt 1740 ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat 1800 cggcgggggt cataacgtga ctcccttaat tctccgctca tgatcttgat cccctgcgcc 1860 atcagatcct tggcggcaag aaagccatcc agtttacttt gcagggcttc ccaaccttac 1920 cagagggcgc cccagctggc aattccggtt cgcttgctgt ccataaaacc gcccagtcta 1980 gctatcgcca tgtaagccca ctgcaagcta cctgctttct ctttgcgctt gcgttttccc 2040 ttgtccagat agcccagtag ctgacattca tccggggtca gcaccgtttc tgcggactgg 2100 ctttctacgt gttccgcttc ctttagcagc ccttgcgccc tgagtgcttg cggcagcgtg 2160 aagctgatcc gtcgagatct gaattcgtag ggataacagg gtaattccct taattctccg 2220 ctcatgatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt gacaggatat 2280 attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata tttaaaaggg 2340 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc 2400 gggagtgctt ggcattccgt gcgataatga cttctgttca accacccaaa cgtcggaaag 2460 cctgacgacg gagcagcatt ccaaaaagat cccttggctc gtctgggtcg gctagaaggt 2520 cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg gacgtagcgc agcgccgaaa 2580 aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat ctgcttgtcg ctctttcggc 2640 cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac aggatgatct ggcgcgagtt 2700 gctggatctc gccttcaatc cgggcctgtg gcgggaactc cacgaaaata tccgaacgca 2760 gcaagatcgt cgatcctccg gcgttcagcc tgtgccacag ccgacaggat ggtgaccgaa 2820 tcctacaagg tagaatccgc ctgagtcgca agggtgactt cgcctatatt ggacgacggc 2880 gcgcagaggg cgacctcttt ttgggttacg attgtaggat tatcactaaa acaatacatg 2940 aacatattca aatggcaatc tctctaaggc attggaaata aatacaaata acagttgggt 3000 ggagtttttc gacctgaggg cgttaacctt ctgttaacct aaaagctctt gcccaaacag 3060 cagaatcggc gctaattgcc agcggcggaa cttttccagt ttcgcgaaaa gtatcgccac 3120 tggcaaggaa tgggtttgag atggcgaagt ctgtcctaaa agcagcgcct gtagttgtag 3180 ggttgacggc cttgatggag cgtcatgccg atgccctctc gagccaactt caagcacatc 3240 atcttaaggt tttcccgccg cattccgaga agggcattcg aacattcggg ccatcggagg 3300 cgtccaagct gctcggcgtt ggcgagtcat atttacggca gaccgcgtct gagatgccag 3360 agttgaatgt tagcatgagc ccgggtggca ggcgaatgtt ctcaattgaa gatatccatg 3420 tgattcggaa gtatatggat caggtcggcc gcgggaaccg gcgctacctg ccacatcgtc 3480 gaggcggcga gcagcttcag gttatctctg tgatgaattt caaaggtggg tcgggtaaga 3540 ccaccaccgc cgcgcatctg gcgcagtacc tcgctatgcg cggatatcga gtcttggcca 3600 ttgatctcga tcctcaagcg agcctttctg cactctttgg gagccaaccg gagacggacg 3660 ttggcccgaa cgaaacgctc tacggcgcta taaggtatga tgatgagcag gtggcaatcg 3720 aacgagtcgt ccgagggact tacattcccg acctccacct gattcctggt aaccttgagc 3780 tgatggagtt tgaacacgat acgccacgcg cgctgatgaa ccgcaaagag ggcgacacgc 3840 tcttttatgg tcgcatcagc caagtaattg aagatatcgc ggataactat gacgtcgtgg 3900 tcatcgactg ccctccccag cttgggtatc tcacgctatc cgcattgact gcggcgacgt 3960 ccattcttgt cacggtccat ccgcagatgc tggatgtgat gtcgatgaac cagtttctgg 4020 caatgacatc gaaccttttg cgtgaaatcg agaatgctgg cgccaagttc aagtttaatt 4080 ggatgcgcta tctgataacc cgtttcgaac cgagcgacgg accacagaac caaatggtag 4140 gttatctgcg gtcgattttt ggcgaaaatg tcctcaattt tccgatgctt aaaaccaccg 4200 cggtttcgga cgctggcctg acaaaccaga ctctattcga agtggagcgt ggcctgttca 4260 cgcgctcgac ctatgatcga gccttggagg cgatgaacgc cgtcaacgac gagatcgaaa 4320 cactgatcaa aaaagcatgg ggtaggccca catgagccgg aagcacatcc ttggcgtctc 4380 aactgacgcc cctgagacgt cgcccgccga caataggacg gcaaagaacc gctccatgcc 4440 gctcctcggc gtaacaagga aggagcgcga tccggcaacg aagctcacag cgaacattgg 4500 taacgcactg cgagagcaaa acgatcgtct tagccgtgcc gaagagatcg agcggcgtct 4560 cgctgaaggt caggcagtga tagagttgga tgcctcgtca atagaaccgt ctttcgtgca 4620 ggatcgtatg cgaggggaca ttgacgggct ccttacttcg atccgggaac aaggacagca 4680 agtcccaatc cttgtgcgac cgcatccgag ccagccgggc cgatatcagg ttgccttcgg 4740 ccaccgccgg ctacgcgccg tttcagaact cggacttccg gtcagagcgg tcgttcgcga 4800 actgacggac gagcaagtgg tcgtagcaca gggtcaggaa aacaatgagc gcgaagatct 4860 taccttcatc gaaaaggcgc gcttcgcaca tcgcctgaac aggcagtttt ctcgagagat 4920 tgtcatcgcc gcgatgtcga tcgacaagag caatttgtcc aagatgcttc tgctcgtcga 4980 cgccctcccc tctgaactga ccgatgctat tggtgccgct cctggtgttg gacggccgag 5040 ttggcaacaa cttgccgagc tgattgagaa agtttcttca ccggccgacg tggctaaata 5100 tgctatgtcg gaggaagttc aagcgctgcc atcggcagaa cgattcaagg cggtgatcgc 5160 tagtctgaag cccagtcggg ttgcgcgtgg acttcccgag gtcatggcca ccccagacgg 5220 caccagaatt gcacaggtga cgcagagcaa ggccaaactg gaaatcacga ttgacaggaa 5280 ggcgacgccc gattttgcga ccttcgtgct cgatcatgtg ccagcgctgt atcaagcgta 5340 ccacgctgag aaccaacgga aacggggaga gtaaaccgca aaagaaaaga gccccctcaa 5400 cgtcgccgtc gcggaagccc ttctgtctct ctagcgcgaa cagaatcgca tttcctcgaa 5460 tcctcgtcaa gagtttttag cgccgttttg gtgagctgat ttcctttgcc tgctgaaagg 5520 tgaaagatga tgcagacagg aagtgtaacg acgccattcg ggcggcggcc aatgacgctt 5580 gcgcttgtgc ggcgccagac ggcgctggcc gatatcaaac aaggcaagac agcggacaag 5640 tggaaggtct ttagagacgc gtccgcggcc atggaactac ttggaatcca gtccaacagt 5700 cttgccgtcc ttgatgcgct attgagcttt cacccggaaa cggagttgcg tcaggaggca 5760 cagctgatcg tcttcccgtc gaatgctcag cttgcccttc gggcgcatgg gatggctggc 5820 gcgactttgc gtaggcacat cgccatgctc gtggagtcag gcttgatcgt ccggaaggat 5880 agcgccaacg gaaagcgtta cgctcgtaag gatggcgctg gtcagatcga gcgcgcgttt 5940 ggcttcgatt tgtctccgct tctcgcgcgg tccgaagagc tagcgatgat ggcacagcag 6000 gtgatggccg atcgagcagc attcaggatg gccaaagaaa gtctgacgat ttgccgacgg 6060 gacgttcgga agctaattac ggcagctatg gaagagggag cggagggcga ctggcaagct 6120 gtcgaggaag tctatgtgga acttgtgggt agaattccac gcgccccgac gcttgctgat 6180 gtagagtcaa ttctcgaaga gatgtggatg ctccaggaag agataatcaa ccggttggaa 6240 attagagaca attcagaaaa taatagcacc aatgctgccc agagcgagca gcacatacag 6300 aattcaaaac ccgaatccgt taatgaactt gaacctcgct ctgaaaagga gcagggcgct 6360 aagccgagtg aaatagaccg ggcagggagc gagccgataa aagcgttccc cctcgggatg 6420 atcctgaaag catgcccgac cattggcaat tatgggccga gcggtgcggt tgctagctgg 6480 cgtgacctca tgtcggctgc ggtggtggtt cggtctatgc tgggggtcag cccgtcggct 6540 taccaagacg cgtgtgaggc aatgggaccg gagaatgcgg cagcagcgat ggcgtgcatt 6600 ttggagcgag cgaacttcat caattcgccc gggggctatc tccgagatct gacacggcgg 6660 agcgagctcg ggaagttttc acttggcccg atgataatgg cgctcttgaa ggctagcggg 6720 caggggacgt tgcggtttgg ctagaattag cgagtatgga gcaggatggt ctgtggtcag 6780 ctgaccacag acctaatagg ttgaaaacat gagcgttttt tggatgatcg acagaccatc 6840 cgattcccgg agtaccaagc gtgctctgat gggagcgata acattactca acaagcacga 6900 aggccccatg ccgatcgttg atcgtgaagg agagcctgct ctacatgcgg cggtattttg 6960 ccggccgagg catgtagtcg cggagcactg cctatttact gccctaggca caaacgttga 7020 ctcttggatc gagctggcag acaaagcaat aacccacaca gaggacgatt aatggctgac 7080 gaagagatcc agaatccgcc ggacggtact gctgctgccg aagttgagcc ggctgctcct 7140 agaggtagaa gagcaaagaa agcaccagcc gaaacagccc gcacgggatc gttcaaatcc 7200 gtgaagccga aaacccgcgg cctcagcaac cgagaaaaac tggagaagat cggtcaaatc 7260 gaagctcagg tcgctggcgg cgcaaccttg aaggacgccg ttaagatcgt gggtatttcc 7320 gttcagacct attatcaatg gaagagagct gcggttcaac ctgtctcaca gaatccggcc 7380 gtgtctgttt cagttgacga tgaactcggc gagttcatcc aactcgagga ggaaaatcgg 7440 cggctcagaa agctacagct tctctctgaa aaattgcgcg cagaaaacgc ggacttacgc 7500 aaaagacttg gaatcaacta gcggcctttg ggggtccctc gctaaatcaa cttgacgcgc 7560 atctctcaat gaatgaatgc cttggaaaat ttccgcgcat tctgatcgtt gcgctatcaa 7620 gcaatcccaa tcttctggca tatgaaaaaa ccacgaccca aattcgtcgt cgaatataag 7680 acgaacaggc gacaagccaa ggcccgacca acgtcaatct ggggcaatct gggtttgcag 7740 gcggtgggcc gtgcggtcgc ggccgacgga gtcgtgccga agagtagtct gccgcaagtc 7800 cgttccgtcc tggagaaggt tgtcgctgca gatccagacg tggctgccat ctttgctcag 7860 gttgagatta agggccttcc ggctcccccc aatacatcat caccgaacga gggtcacatc 7920 gagccggtag ttattgaggg caaggtgttt gccgcgcctt tcgtgcagga ggagcgcacc 7980 ttggccccgc cgcaggctcc gagatctcga gcgaaaccgc ggacaaaaag gcggtccccg 8040 gacaccacag aacgtcctgc agtccaaggt taccgtcgtg gtccctcggt gctgccaggt 8100 tttgaggaag agcttgccgc tatcgaggcg gaaaaccgtc gtctgaagcg cctcttgatc 8160 gtgaagctcc gtgaggagaa cgacaggctt aagttcgcgc ttcgacgatt tgggggcgcc 8220 tgagcacgat gctggcccac ttgcttcagc gggcaaacaa catggagtgg agatctaatg 8280 atcgcggcgc ttgaacatcg ctaggggtct tcggcgccgc tgaaccgtgc tccattttgc 8340 actcggagac acagcctccc aaaaacctgg cgccgctcgt tggtgaactc ctcaacatcg 8400 agcggatcaa gggaaactgg aatgacatcc ttcgacccgt cacggcgatc cgctctggcc 8460 aggtccaacc tacggccatg cccgcaaagc caacggcatt tccacgtcaa gcacattgac 8520 ccactcggct gacggtacat cagctggcga gctcatccgg acccagacga actgcgttga 8580 tctcaggccg ttgcggcaga aaacctccat cctcgcagcc gatccaacac tttcctgtgg 8640 taatgcctat ttcagatgaa acagcctttt ttaaacttgg cgcgtcattc tgatattatg 8700 gccacacctc tacgatctag ggagtgcgag aagtggaaag aaatgaagaa cccgatttgc 8760 acacacatac ctcgatgcgg aataaaaatg catcatcgct tggtgaggcc ctggatgacg 8820 atgcgatcgc caacatcgcg catcacattg ttacagacga cccggtggaa acgtcaaaaa 8880 acttcacaaa acttaagctg attaacaaat cagtgaaaca gggtttcgaa cgcagtgcgt 8940 acggaaaatt ccatcgacgc attagccggc ttggtattgc tagtaaggcg ctatatgaca 9000 acgctgtgcc gaaggaagga ttcgtagaac atcctgaata tgctccagct ctgcaaaggg 9060 attatgcgtt ggcaggagag cgaattgagg cggttggtgg aactttcagg ttgcaaacac 9120 ctcagcggaa atcggctctt gtagatcata tcttgagtat gcccgaaggt ttcgatcaag 9180 ctcatgccct tgagtctata gcccctcatg ttggcgatct aagcgcggaa gatcgccatc 9240 gtgttgtgaa caaaatgctc gagcactttg ccaccccgtt tcaagaaggg gggctgccaa 9300 ccgacgaaaa tttttgtgga acgtacgctt tgtccaaggc gtttcctcat atggaagaca 9360 gcctaaaagc aaagatgctg gatgtggtgt tagagcatcc tcacttagcc aacctccatt 9420 ggacgcagag agaactagtt gaagggtggc atcaagaccg tgaagctgag aagtcagcct 9480 ggcgttccaa tcaaaagcca attgtcgagg ggcgacttgc agacctcgaa cgatcgatcc 9540 acagtgatat cgaggtgaca aacaggagtg attttgcgag gatgcgttca gccaggccct 9600 tgcaacaagc tataagcgat acgttcgatc ttgcgcgtga ggagctcaaa agccgccctc 9660 gtgaagggcg gggtcgataa ttacatctat tggtctttct gccgaacctc tatgaaatcc 9720 gcacgtcaaa tgtggctttt acttcccgat ttcaattgaa gcgctaagct gaaaatcccg 9780 tccccatttc tgacctaacg gtttcatcac cggggcaggg agcggcgctt aaaacggtga 9840 cggccaccac caaaggacat gttcgctcac gcggtgcctc cgtgcttctt tgcaagagcc 9900 tgcttcaggc tgatgatctc cttcagtgcg ttggtgtgaa tccgctccag gcgtttcagt 9960 tcgctctcca aataggcgtt gcgcttatcc agcctcgtcc gatcttcttc caagctctcc 10020 attttcacta gcgcgttatg gtaattcaac cgtaactcct caaaaaaagc acgttcggcg 10080 acgaaatggg tttcggaatc gagttcaggt atgtcgccga catgcacgac gcgaaccgcc 10140 ttgccggcag cggcgagccg ggcgcggtaa gcgcgttgaa tttccgcttg ggtcagcgcg 10200 tttggcttgc ggggccgcga ggaaatttgg gagtaaccat aacggttatt tatcataacg 10260 cgttatcact ggatagcatc ggcgcaggcg gatggcgcgc ttcgccgggt cgaaatcaat 10320 atttccagat cgagatggat tgaagacctg aagggccctc agagcccaga cgccttggtt 10380 aggttggtgc ggaaccgatc ccgccgccgc tggtatttgc gcgtcatctc ggccgaggcg 10440 tggcctagtt gtttctgaac gtaacgctcc tcaatgtcgg ccgaggaggc aagaccggcg 10500 cgcagcgagt gccctgagaa cagcagcgcc cgttcccctt ctggaagatc ggaacggacg 10560 ccggcggcga gtgcggtctg cttgaccagg cgggcgacat gcttgtcgga gagccgctcg 10620 acgtccacgg tcttgttgtc cttgaagatg cgccggaaca ggggaccgcg cgcgatcctg 10680 ccgaagcgga tcgtagtgac caccatttgc cccatatcac cgtcggtact gatcccgtcg 10740 tcaataaacc gaaccgctac accctgagca tcaaactctt ttatcagttg gatcatgtcg 10800 gcggtgtcgc ggccaagacg gtcgagcttc ttcaccagaa tgacatcacc ttcctccacc 10860 ttcatcctca gcaaatccag cccttcccga tctgttgaac tgccggatgc cttgtcggta 10920 aagatgcggt tagcttttac ccctgcatct ttgagcgctg aggtctgcct cgtgaagaag 10980 gtgttgctga ctcataccag gcctgaatcg ccccatcatc cagccagaaa gtgagggagc 11040 cacggttgat gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg 11100 ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag 11160 ttcgatttat tcaacaaagc cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta 11220 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt 11280 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga 11340 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac 11400 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga 11460 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcagct tatgcatttc 11520 tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac 11580 caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat cgctgttaaa 11640 aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac 11700 aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat 11760 cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag 11820 aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac 11880 gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata 11940 gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc 12000 atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa tatggctcat 12060 aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt 12120 tttatcttgt gcaatgtaac atcagagatt ttgagacacc gcgcttgcgg aagcatcagc 12180 aaataaggcc agcacagcca gcgcagttgc cgctttggtt cctgattctg ttcttgatga 12240 attaaacaaa gcggcacagt aacaaaggac ttcattgata atttttcttc aggaggaaga 12300 catgtcattc ttttctacgt taaaaacagc tttgtctttg aaggagaaac ttgctgctac 12360 tggtgttctt gttctgattt gcgcacttgt tggtgctggg tttgcatggg aacgtcatca 12420 gctaaagcaa gccatagaga aaattggcag tcttgatcag gctgttaagg aacgtgataa 12480 gtcaataatg gatcttaacc agaccattga gacgatgaac aaagcagagc aacattttca 12540 cagccaggaa gtgaaaaatg aatcagaaca agccaagtat gctgacaggc aaatggaacg 12600 aaaagctgaa gttcagaaac aactggttgc ggcgggtaat gttcgccagc gtattcctgc 12660 tgacactcag cggttgctcc gggagtcgat cagcgaattt aacgccgacg ccgacaaagg 12720 ttaaccaccc tgcccccaaa agtgcattta tgtgtaggat gccagagttt agcagtgaat 12780 attttgatga tctgccagcg tatatcctcg atacagaaac gatgctgatg gggattaaca 12840 ggaagaatcg caacgttaat gattacaacc gagctattag cggtaactaa aagggatttt 12900 tatgtctgat aaagtaacag taaagcaaac tatcaacaaa gcgacttcaa tctacaaaat 12960 tgagcaaatc actgttggca agccaggatc tgaacaatac cgtcgtgctt tcgagcttgc 13020 cgatcagctt ggtttaaaac acccggattg cattgagcat gtatttccga cctatgctga 13080 tgagcaatgt actcatgttc ttaccgaaga ggattttttc agcactgaag aacgagaagg 13140 cgttgatcgc tgcattggtg tgatttgttc ttcggtaagt gatgagttat tccctaatgt 13200 gcctgaatat ggtggtattg gataccaatt cctgtacgag ggcgatgagc ttaaatgcta 13260 tgaacatggt cttctcatcg aaagcgtaga ataatacgac tcccttccaa ccggctacgt 13320 tggccggttt ttcacttatc cacattatcc actggataga tccaataatc aggtccatac 13380 agatcccaat tagatccata tagatccctg atcgttgcag gccgcgccac gtctggctta 13440 gaagtgtatc gcgatgtgtg ctggagggaa aacgatgtgt gctggaggga taaaaatgtg 13500 tgctgacggg ttgctaatgt gtgctggcgg gatataggat gtgtgttgac gggaaagcta 13560 gcttgggtag ttatcaccac ttataaaaac tatccacaca attcggaaaa agtaatatga 13620 atcaatcatt tatctccgat attctttacg cagacattga aagtaaggca aaagaactaa 13680 cagttaattc aaacaacact gtgcagcctg tagcgttgat gcgcttgggg gtattcgtgc 13740 cgaagccatc aaagagcaaa ggagaaagta aagagattga tgccaccaaa gcgttttccc 13800 agctggagat agctaaagcc gagggttacg atgatattaa aatcaccggt cctcgactcg 13860 atatggatac tgatttcaaa acgtggatcg gtgtcatcta cgcgttcagc aaatacggct 13920 tgtcctcaaa caccatccag ttatcgtttc aggaattcgc taaagcctgt ggtttcccct 13980 caaaacgtct ggatgcgaaa ctgcgtttaa ccattcatga atcacttgga cgcttgcgta 14040 acaagggtat cgcttttaag cgcggaaaag atgctaaagg cggctatcag actggtctgc 14100 tgaaggtcgg gcgttttgat gctgaccttg atctgataga gctggaggct gattcgaagt 14160 tgtgggagct gttccagctt gattatcgcg ttctgttgca acaccacgcc ttgcgtgccc 14220 ttccgaagaa agaagctgca caagccattt acactttcat cgaaagcctt ccgcagaacc 14280 cgttgccgct atcgttcgcg cgaatccgtg agcgcctggc tttgcagtca gctgttggcg 14340 agcaaaaccg tatcattaag aaagcgatag aacagcttaa aacaatcggc tatctcgact 14400 gttctattga gaagaaaggc cgggaaagtt ttgtaatcgt ccattctcgc aatccaaagc 14460 tgaaactccc cgaataagtg tgtgctggag ggaaaccgca ttaaaaagat gtgtgctgcc 14520 gggaaggctt gtccaatttc ctgtttttga tgtgcgctgg agggggacgc ccctcagttt 14580 gcccagactt tccctccagc acacatctgt ccatccgctt ttccctccag cacacatcga 14640 agctgccggg caagccgttc tcaccagttg atagagagtg aagctagctt ggctgcccat 14700 tgaagcagga aatcaccaaa atgattcagg ctacaacctg aacgtagaag aaatccgcgt 14760 cctttatgcg tggaggatgc caaagcatgt tgtgacacac ttggcaaagg agtaagcatg 14820 cagagaatgc tatgtacaag catctacgca tacattatta ttttatgcag catttttaat 14880 taaattcaaa aatacagcat aaaggatgac tttcgatgag tgattccagc cagcttcaca 14940 aggttgctca aagagcaaac agaatgctca atgttctgac tgaacaagta cagttgcaaa 15000 aggatgagct acacgcgaac gagttttacc aggtctatgc gaaagcggca ctggcaaaat 15060 tgcctctact gactcgagcg aacgttgact atgccgtaag tgaaatggaa gaaaagggtt 15120 atgttttcga taaacgccct gctggctctt caatgaaata tgcgatgtca attcagaaca 15180 tcattgacat atatgaacat cgcggagtgc caaaataccg ggatcgctac agcgaagcgt 15240 atgtgatttt catctccaat cttaaaggcg gtgtgtcaaa aactgtatcg acggtttctc 15300 tggcgcatgc aatgcgtgct caccctcatc ttcttatgga ggatttaagg attctggtta 15360 ttgaccttga tccgcaatct tcagcaacga tgtttttaag ccataaacac tctattggta 15420 tcgtaaacgc aacatctgca caggctatgt tgcagaatgt aagccgtgaa gagctgttag 15480 aggagtttat tgttccttct gttgtacctg gggttgacgt tatgcctgcg tcgattgacg 15540 atgcctttat tgcatccgat tggagagagc tgtgcaatga gcatctaccg ggtcagaaca 15600 tccatgctgt cctgaaagaa aatgtgattg ataagctgaa gagcgattat gactttatcc 15660 tcgttgatag tggtcctcac cttgacgcct tcctgaaaaa tgctttggcc tcggccaata 15720 tactgtttac acctctgccg ccagcaactg tcgatttcca ctcatcgctt aaatacgttg 15780 cccgccttcc tgagttggtg aaactcattt cggatgaagg ctgcgagtgc cagcttgcga 15840 ctaacattgg ttttatgtcc aagttgagta acaaggcaga ccataagtat tgccatagcc 15900 tggctaaaga agtgttcggt ggggatatgc ttgatgtctt cctccctcgc cttgacggtt 15960 ttgaacgctg cggcgagtct tttgacactg ttatttcagc taacccggca acgtatgttg 16020 gtagtgctga tgcattgaag aacgcgcgaa ttgccgcgga agattttgct aaagcagttt 16080 ttgaccgtat tgaatttatc agatctaact gaggagtaag aaacccccat gtcaaagaaa 16140 aacagaccaa caattgggcg aacccttaat ccttcaatat taagcggatt tgatagttct 16200 tcagcctctg gcgatcgagt cgagcaggta ttcaagttat caactggtcg ccaggccaca 16260 tttattgaag aggtaatacc tccgaaccag gtagaaagcg atacctttgt tgatcagcat 16320 aacaacgggc gtgaccaggc atctcttacg ccaaaatcat taaaaagtat ccgaagcact 16380 attaagcatc agcaatttta ccctgcaata ggtgttagac gggctacagg gaaaattgaa 16440 attttggatg gttcccggcg tcgagcttct gccatcttag agaacgtagg gttgcgggtt 16500 ttagtcacgg accaggagat cagcgttcag gaagcgcaaa atttagcgaa agacgttcag 16560 acagcattgc agcacagcat tcgagaaata ggtctgcgtt tgatgcgaat gaaaaatgat 16620 gggatgagtc agaaggatat tgcagccaaa gaagggctgt ctcaggcgaa ggtcacgcgt 16680 gctctccagg cagcgagtgc tccggaagaa ttagtcgccc ttttccctgt gcagtcggaa 16740 ttaacctttt cggactacaa aacgctttgt gctgttggcg acgaaatggg gaacaagaat 16800 ttagagtttg atcagcttat tcaaaacata tccccggaaa taaacgacat cttatccatt 16860 gaagaaatgg ccgaagatga agttaaaaat aaaatcctgc gcttgataac aaaggaagcc 16920 tcactactca cggataaagg ttctaaagat aagtccgtag ttactgaatt atggaaattt 16980 gaggacaagg atcgctttgc aaggaagcgc gtgaaaggcc gtgcattttc ttatgagttt 17040 aatcgactct caaaagagtt acaggaagaa ctcgacagga tgattgggca tatccttaga 17100 aagagcctcg ataaaaagcc gaagccttaa actttcgcca ttcaaatttc actattaact 17160 gactgttttt aaagtaaatt actctaaaat ttcaaggtga aatcgccacg atttcacctt 17220 ggattttacc ttcctcccct cctcccgaaa aaaataaaaa aattgcttgt cacgagaaag 17280 tcaacaagtg actttcaata aaatctcttc cgaaaaggga ttcacacaag tgccttgtgt 17340 ttaaggaaga gtaaattgag taacttacgc gaataccaga atcgtattgc agatatcgca 17400 aaacgctcta aagctgtgct tggctgggca agcactgcgc agttcggtac tgataaccaa 17460 ttcattaaag atgatgccgc gcgtgccgca tctatccttg aagctgcacg taaagacccg 17520 gtttttgcgg gtatctctga taatgccacc gctcaaatcg ctacagcgtg ggcaagtgca 17580 ctggctgact acgccgcagc acataaatct atgccgcgtc cggaaattct ggcctcctgc 17640 caccagacgc tggaaaactg cctgatagag tccacccgca atagcatgga tgccactaat 17700 aaagcgatgc tggaatctgt cgcagcagag atgatgagcg tttctgacgg tgttatgcgt 17760 ctgcctttat tcctcgcgat gatcctgcct gttcagttgg gggcagctac cgctgatgcg 17820 tgtaccttca ttccggttac gcgtgaccag tccgacatct atgaagtctt taacgtggca 17880 ggttcatctt ttggttctta tgctgctggt gatgttctgg acatgcaatc cgtcggtgtg 17940 tacagccagt tacgtcgccg ctatgtgctg gtggcaagct ccgatggcac cagcaaaacc 18000 gcaaccttca agatggaaga cttcgaaggc cagaatgtac caatccgaaa aggtcgcact 18060 aacatctacg ttaaccgtat taagtctgtt gttgataacg gttccggcag cctacttcac 18120 tcgtttacta atgctgctgg tgagcaaatc actgttacct gctctctgaa ctacaacatt 18180 ggtcagattg ccctgtcgtt ctccaaagcg ccggataaag gcactgagat cgcaattgag 18240 acggaaatca atattgaagc cgctcctgag ctgatcccgc tgatcaacca cgaaatgaag 18300 aaatacaccc tgttcccaag tcagttcgtt atcgcggctg agcacacggt acaggcggcg 18360 tatgaagcac attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 18420 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 18480 cctgtaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg 18540 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc 18600 atgattacgc caagctagct tgcatgcctg caggtcgaag gccgtggcgg cggcactttt 18660 gcccatgaat tgctcgctgt ggagtacgca ggctggattt ctcccgcgtt tcggctgaag 18720 gtaaaccaga catttatcga ctatcgaacc ggaagattac aacctgctat tccgcagagt 18780 ctccctgaag ctctccgttt ggctgccgac ctggcagagc aaaagcaacg gctggagcaa 18840 aaaatgctta tggatgcacc taaagtcgaa ttcgctgaac gcgttgctac cgccagcggg 18900 gttctaatcg gcaactatgc caaagtgctc ggcctgggcc aaaactatct cttcacctgg 18960 ttgcgtgata acggaattct gatcgcaacc ggtgaacgca ggaacgtccc caaacaagaa 19020 tacatatctc gtgggtattt cacccttaaa gaaaccgtga tcgatacaag caatggaagc 19080 aggatttctt tcacgactcg tataaccggc aaaggtcagc agtggctgat gaagcgattg 19140 cttgatgctg gtgtgctgct gtcgcggcaa cgcgctaaca gacgtagtaa gaaccaccag 19200 cattgtaatg ctggctaaag tcactttcct gagctgtata acgatgagcg attttacttt 19260 ttctggctat gaattggcct gctttgtaac acactccggt ctatcccgta gcgccgggca 19320 tatcctgtcg caatgtgcaa atctcgcggc aacaaccagt gaatacttca ttcacaagcc 19380 tcaccgcctg atcgcggcag aaactggtta tagccaatca accgtcgttc gtgcattccg 19440 tgaagctgta aacaaaggaa ttctgtctgt agagattgtt atcggcgatc accgtgaacg 19500 tcgcgctaac ctgtaccggt ttacaccatc ctttttggcc ttcgcacaac aagccaaaaa 19560 tgcgctgata gaaagcaaat taaagatctc ttcagcggca accaaggtta aagctgttct 19620 cgctaagaca ttggctttat ttaatttttt atccacaccc ccatgtcaaa atgatacccc 19680 ctccccctgt caggatgacg tggcaataaa gaataagaag tcacaagtta aaaaaacaaa 19740 aagatcagtt tccggcggtg ccggaacaac cagcctcaaa aaattgactt catggatcgc 19800 taaggcaaaa gcaaaggctg acaatctgcg gttatccaaa aaacgcactc aaaaacatga 19860 gttcaagcag aaagtagagg cggctgcgcg gaaatatgct tacctgaaga acaagcgttc 19920 gcctgatatt ggcgggatat caaacttcga taacctaccg cattgcatga cggtaaacga 19980 agctcttaat gcggttttag ccaaaaataa agataacgaa caatggggta taccggcagg 20040 attcagaggg taatgaattg ctctaattat aaccatgcat actttcaaca cctctagttt 20100 gccatgaggc aaactcatag gtgtcctggt aagaggacac tgttgccaaa actggacgcc 20160 ccattattgc aattactaag aaaccattat tatcatgaca ttaacctata aaaataggcg 20220 tatcacgagg ccctttcgtc ttcaagaatt ttataaaccg tggagcgggc aatactgagc 20280 tgatgagcaa tttccgttgc accagtgccc ttctgatgaa gcgtcagcac gacgttcctg 20340 tccacggtac gcctgcggcc aaatttgatt cctttcagct ttgcttcctg tcggccctca 20400 ttcgtgcgct ctaggatcga cgatcgtcaa cgttcacttc taaagaaata gcgccactca 20460 gcttcctcag cggctttatc cagcgatttc ctattatgtc ggcatagttc tcaagatcga 20520 cagcctgtca cggttaagcg agaaatgaat aagaaggctg ataattcgga tctctgcgag 20580 ggagatgata tttgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc 20640 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca 20700 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga 20760 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt 20820 ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata 20880 acacattgcg gacgttttta atgtactgaa ttcttaatta acccacgtgt tgagaaataa 20940 aagaaaatgc caatgaagta tcggcatttt ctttttgctg ttattagttg actgtcagct 21000 gtccttgctc caggatgctg tttttgacaa cggatgtttt attgcctttg atgttcatta 21060 agaagctcgg ggcaaatgtt gcctttttat cctcgaagaa gcctctgttt gtcatgtagc 21120 ttgtgataac cacattgttg cctttggctt gcggcactgc gaagtgagag taagtgaatg 21180 tcacatcgtt tggatcaaga cccatttgca gcacaagccc tgttttgttc agcggcttgt 21240 aagggccggt taaagagttt gatacataac caagcatgta aatatcgttt gagttaatac 21300 catcgatcgt catttttgaa ccgcgtgaat cagtgaacaa gtaccatttg ccgttcattt 21360 tgaaaacatt cgcgcgctcg atttcatcag ttaccgtgtt tgaagtgatc agcggcttca 21420 ttactttttt caatgtgtaa tcattattta actctatgat accgagggcg ccgttcgcta 21480 actcagcatc gcgtttttta gcgctctgct ggagcttctg gctttcttta cggaagaagt 21540 tcgtgccgcc gccgtagtac gctttgttaa ataaagattc ttcgccttgg tatccgtttt 21600 ctgttcccgt gttggcttcg aatacaagat atttatggcc tttgtcttca acgtagtgag 21660 ggtctctcag cgtatggttg tcgccggatg tataattgcc ttcatcgata aactgctgaa 21720 cgttctgata tgtttttccg tctccgtcaa aaatcgtttt gtgatcttcc actccgttga 21780 ttttgagtgt gtcatcagat tttgacacat ttacctgcgc tgttgtcagg ctttgtttgc 21840 cgtaatgttt accggaatag tcagtgtaga ataaacggat ttttccgtca gatgtaaagg 21900 ttgcagaacc ggaccattct tgcgtctgat ctttcaggat cggatcgttg gcgtcgaact 21960 tatcgctgtc tttaaagaca cggcccgcgt ttttccagct gtcgattgag ttgtcgccga 22020 ccttttgata aaacatgtag attgatgtgt catcagcgtc tttcgggctt cccgcaagag 22080 caaacacaac gtgatagccg ttgtattcag ctactgttcc gtcagcgttt tgcagcggcc 22140 agctgtccca cacatcaagt ccttttgcag actcaatatt tttaatcgtt gattgatcga 22200 attgaggcac ttggtatttt tcgttttgct gctgtttagg gatctgcagc atatcatggc 22260 gtgtaatatg agagacgccg tacgtttctt tgtatgcttt ttggttattt tctttcgcga 22320 aggcttgagt cgctcctcct gccagaagtg cagtcgtaaa agtcagaact gtggcttgtt 22380 ttacaatttt tttgatgttc atgttcatgt ctccttctgt atgtactgtt ttttgcgatc 22440 tgccgtttcg atcctcccga attgactagt gggtaggcct aattgcggcc tagggataac 22500 agggtaatgc ggccgcggcc ggccgtcgac 22530 // ID AB025788; SV 1; linear; other DNA; STD; SYN; 5260 BP. XX AC AB025788; XX DT 05-OCT-1999 (Rel. 61, Created) DT 14-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Expression vector pLED-HB TTHPOLA, bla genes for Tth DNA polymerase, DE beta-lactamase, complete cds. XX KW beta-lactamase; Tth DNA polymerase. XX OS Expression vector pLED-HB OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-5260 RA Matsuura T., Yomo T., Yamaguchi M.; RT ; RL Submitted (05-APR-1999) to the INSDC. RL Tomoaki Matsuura, Universitaet Zurich, Biochemisches Institut; RL Winterthurerstr. 190, CH-8057 Zurich, Switzerland RL (E-mail:TMatsuura@biocfebs.unizh.ch, Tel:+41-1-635-5583, RL Fax:+41-1-635-5712) XX RN [2] RA Matsuura T., Yamaguchi M., Yomo T., Miyamoto C., Urabe I.; RT "An in vitro self-encoding system"; RL Unpublished. XX DR MD5; 2af3150e0068f63b3f910793b0e8f900. DR EuropePMC; PMC124265; 12032314. XX FH Key Location/Qualifiers FH FT source 1..5260 FT /organism="Expression vector pLED-HB" FT /mol_type="other DNA" FT /db_xref="taxon:98931" FT CDS 1..2505 FT /codon_start=1 FT /transl_table=11 FT /gene="TTHPOLA" FT /product="Tth DNA polymerase" FT /protein_id="BAA84999.1" FT /translation="MEAMLPLFEPKGRVLLVDGHHLAYRTFFALKGLTTSRGEPVQAVY FT GFAKSLLKALKEDGYKAVFVVFDAKAPSFRHEAYEAYKAGRAPTPEDFPRQLALIKELV FT DLLGFTRLEVPGYEADDVLATLAKKAEKEGYEVRILTADRDLYQLVSDRVAVLHPEGHL FT ITPEWLWEKYGLRPEQWVDFRALVGDPSDNLPGVKGIGEKTALKLLKEWGSLENLLKNL FT DRVKPENVREKIKAHLEDLRLSLELSRVRTDLPLEVDLAQGREPDREGLRAFLERLEFG FT SLLHEFGLLEAPAPLEEAPWPPPEGAFVGFVLSRPEPMWAELKALAACRDGRVHRAADP FT LAGLKDLKEVRGLLAKDLAVLASREGLDLVPGDDPMLLAYLLDPSNTTPEGVARRYGGE FT WTEDAAHRALLSERLHRNLLKRLEGEEKLLWLYHEVEKPLSRVLAHMEATGVRRDVAYL FT QALSLELAEEIRRLEEEVFRLAGHPFNLNSRDQLERVLFDELRLPALGKTQKTGKRSTS FT AAVLEALREAHPIVEKILQHRELTKLKNTYVDPLPSLVHPRTGRLHTRFNQTATATGRL FT SSSDPNLQNIPVRTPLGQRIRRAFVAEAGWALVALDYSQIELRVLAHLSGDENLIRVFQ FT EGKDIHTQTASWMFGVPPEAVDPLMRRAAKTVNFGVLYGMSAHRLSQELAIPYEEAVAF FT IERYFQSFPKVRAWIEKTLEEGRKRGYVETLFGRRRYVPDLNARVKSVREAAERMAFNM FT PVQGTAADLMKLAMVKLFPRLREMGARMLLQVHDELLLEAPQARAEEVAALAKEAMEKA FT YPLAVPLEVEVGMGEDWLSAKG" FT CDS 3194..4054 FT /codon_start=1 FT /transl_table=11 FT /gene="bla" FT /product="beta-lactamase" FT /protein_id="BAA85000.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" XX SQ Sequence 5260 BP; 1125 A; 1542 C; 1543 G; 1050 T; 0 other; atggaggcga tgcttccgct ctttgaaccc aaaggccggg tcctcctggt ggacggccac 60 cacctggcct accgcacctt cttcgccctg aagggcctca ccacgagccg gggcgaaccg 120 gtgcaggcgg tctacggctt cgccaagagc ctcctcaagg ccctgaagga ggacgggtac 180 aaggccgtct tcgtggtctt tgacgccaag gccccctcct tccgccacga ggcctacgag 240 gcctacaagg cggggagggc cccgaccccc gaggacttcc cccggcagct cgccctcatc 300 aaggagctgg tggacctcct ggggtttacc cgcctcgagg tccccggcta cgaggcggac 360 gacgttctcg ccaccctggc caagaaggcg gaaaaggagg ggtacgaggt gcgcatcctc 420 accgccgacc gcgacctcta ccaactcgtc tccgaccgcg tcgccgtcct ccaccccgag 480 ggccacctca tcaccccgga gtggctttgg gagaagtacg gcctcaggcc ggagcagtgg 540 gtggacttcc gcgccctcgt gggggacccc tccgacaacc tccccggggt caagggcatc 600 ggggagaaga ccgccctcaa gctcctcaag gagtggggaa gcctggaaaa cctcctcaag 660 aacctggacc gggtaaagcc agaaaacgtc cgggagaaga tcaaggccca cctggaagac 720 ctcaggctct ccttggagct ctcccgggtg cgcaccgacc tccccctgga ggtggacctc 780 gcccaggggc gggagcccga ccgggagggg cttagggcct tcctggagag gctggagttc 840 ggcagcctcc tccacgagtt cggcctcctg gaggcccccg cccccctgga ggaggccccc 900 tggcccccgc cggaaggggc cttcgtgggc ttcgtcctct cccgccccga gcccatgtgg 960 gcggagctta aagccctggc cgcctgcagg gacggccggg tgcaccgggc agcagacccc 1020 ttggcggggc taaaggacct caaggaggtc cggggcctcc tcgccaagga cctcgccgtc 1080 ttggcctcga gggaggggct agacctcgtg cccggggacg accccatgct cctcgcctac 1140 ctcctggacc cctccaacac cacccccgag ggggtggcgc ggcgctacgg gggggagtgg 1200 acggaggacg ccgcccaccg ggccctcctc tcggagaggc tccatcggaa cctccttaag 1260 cgcctcgagg gggaggagaa gctcctttgg ctctaccacg aggtggaaaa gcccctctcc 1320 cgggtcctgg cccacatgga ggccaccggg gtacggcggg acgtggccta ccttcaggcc 1380 ctttccctgg agcttgcgga ggagatccgc cgcctcgagg aggaggtctt ccgcttggcg 1440 ggccacccct tcaacctcaa ctcccgggac cagctggaaa gggtgctctt tgacgagctt 1500 aggcttcccg ccttggggaa gacgcaaaag acaggcaagc gctccaccag cgccgcggtg 1560 ctggaggccc tacgggaggc ccaccccatc gtggagaaga tcctccagca ccgggagctc 1620 accaagctca agaacaccta cgtggacccc ctcccaagcc tcgtccaccc gaggacgggc 1680 cgcctccaca cccgcttcaa ccagacggcc acggccacgg ggaggcttag tagctccgac 1740 cccaacctgc agaacatccc cgtccgcacc cccttgggcc agaggatccg ccgggccttc 1800 gtggccgagg cgggttgggc gttggtggcc ctggactata gccagataga gctccgcgtc 1860 ctcgcccacc tctccgggga cgaaaacctg atcagggtct tccaggaggg gaaggacatc 1920 cacacccaga ccgcaagctg gatgttcggc gtccccccgg aggccgtgga ccccctgatg 1980 cgccgggcgg ccaagacggt gaacttcggc gtcctctacg gcatgtccgc ccataggctc 2040 tcccaggagc ttgccatccc ctacgaggag gcggtggcct ttatagagcg ctacttccaa 2100 agcttcccca aggtgcgggc ctggatagaa aagaccctgg aggaggggag gaagcggggc 2160 tacgtggaaa ccctcttcgg aagaaggcgc tacgtgcccg acctcaacgc ccgggtgaag 2220 agcgtcaggg aggccgcgga gcgcatggcc ttcaacatgc ccgtccaggg caccgccgcc 2280 gacctcatga agctcgccat ggtgaagctc ttcccccgcc tccgggagat gggggcccgc 2340 atgctcctcc aggtccacga cgagctcctc ctggaggccc cccaagcgcg ggccgaggag 2400 gtggcggctt tggccaagga ggccatggag aaggcctatc ccctcgccgt gcccctggag 2460 gtggaggtgg ggatggggga ggactggctt tccgccaagg gttagggggg ccctgccgtt 2520 tagaggaagt tcaaggggtt gtccctcaga aacgcctcca ggggaacgcc ctctgggcct 2580 accaggaggc ctttagcccc aaaggtgcgg gtgaaggctt ccaggccctg ggttcttttt 2640 gagggggcgc ttttgacctc gactctagag agcttggctg ttttggcgga tgagagaaga 2700 ttttcagcct gatacagatt aaatcagaac gcagaagcgg tctgataaaa cagaatttgc 2760 ctggcggcag tagcgcggtg gtcccacctg accccatgcc gaactcagaa gtgaaacgcc 2820 gtagcgccga tggtagtgtg gggtctcccc atgcgagagt agggaactgc caggcatcaa 2880 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 2940 aacgctctcc tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg 3000 cccggagggt ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag 3060 gccatcctga cggatggcct ttttgcgttt ctacaaactc ttttgtttat ttttctaaat 3120 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg 3180 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc 3240 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga 3300 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga 3360 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg 3420 cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc 3480 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac 3540 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact 3600 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca 3660 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg 3720 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact 3780 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg 3840 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg 3900 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat 3960 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc 4020 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat 4080 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt 4140 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 4200 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 4260 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 4320 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 4380 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 4440 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 4500 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 4560 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg 4620 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 4680 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 4740 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 4800 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 4860 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 4920 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 4980 cgaggaagcg gcttatcgac tgcacggtgc accaatgctt ctggcgtcag gcagccatcg 5040 gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc gctcaaggcg 5100 cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg caaatattct 5160 gaaatgagct gttgacaatt aatcatcggc tcgtataatg tgtggaattg tgagcggata 5220 acaatttcac acaggaaaca gaattcagga ggtttatcat 5260 // ID AB027254; SV 1; linear; other DNA; STD; SYN; 39902 BP. XX AC AB027254; XX DT 02-JUN-1999 (Rel. 59, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 4) XX DE Cloning vector pSB4 tetR, tetA, hygromycin resistance genes with DE Agrobacterium tumefaciens DNA insert. XX KW . XX OS Cloning vector pSB4 OC other sequences; artificial sequences; vectors. XX OS Agrobacterium tumefaciens OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; OC Rhizobium/Agrobacterium group; Agrobacterium; OC Agrobacterium tumefaciens complex. XX RN [1] RP 1-39902 RA Komari T., Kuraya Y., Murai N., Ueki J., Saito Y.; RT ; RL Submitted (10-MAY-1999) to the INSDC. RL Jun Ueki, Japan Tobacco Inc., Plant Breeding and Genetics Research Lab; 700 RL Higashibara, Iwata, Shizuoka 438-0802, Japan RL (E-mail:Jun.Ueki@pbgrl.jti.co.jp, Tel:81-538-32-7111, Fax:81-538-32-8700) XX RN [2] RX DOI; 10.1046/j.1365-313X.1996.10010165.x. RX PUBMED; 8758986. RA Komari T., Hiei Y., Saito Y., Murai N., Kumashiro T.; RT "Vectors carrying two separate T-DNAs for co-transformation of higher RT plants mediated by Agrobacterium tumefaciens and segregation of RT transformants free from selection markers"; RL Plant J. 10(1):165-174(1996). XX DR MD5; d7e8da78f1677afc121ff556199ed621. XX FH Key Location/Qualifiers FH FT source 1..39902 FT /organism="Cloning vector pSB4" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:94002" FT source 4054..15840 FT /organism="Agrobacterium tumefaciens" FT /mol_type="other DNA" FT /db_xref="taxon:358" FT repeat_region 494..516 FT /note="right border repeat" FT CDS complement(943..1968) FT /codon_start=1 FT /transl_table=11 FT /product="hygromycin B phosphotransferase" FT /protein_id="BAA78025.1" FT /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG FT YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP FT ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ FT TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF FT GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG FT NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK FT E" FT repeat_region 2586..2610 FT /note="left border repeat" FT misc_feature 4054..5359 FT /note="virC operon" FT misc_feature complement(5470..6273) FT /note="virG operon" FT misc_feature 6405..15840 FT /note="virB operon" FT rep_origin complement(18760..18879) FT /note="ColE1 origin of replication" FT misc_feature 19619 FT /note="cos site" FT CDS complement(21325..21975) FT /codon_start=1 FT /transl_table=11 FT /gene="tetR" FT /protein_id="BAA78026.1" FT /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF FT RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR FT PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE FT QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD" FT CDS 22081..23280 FT /codon_start=1 FT /transl_table=11 FT /gene="tetA" FT /protein_id="BAA78027.1" FT /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY FT GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI FT VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF FT FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM FT QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM FT LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL FT AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR FT " FT misc_feature complement(23911..26100) FT /note="Rep operon" FT oriT complement(29483..29849) FT /note="origin of transfer" FT misc_feature complement(31466..37736) FT /note="Ctl, central control operon" FT rep_origin complement(39390..39840) FT /note="oriV, around this region" XX SQ Sequence 39902 BP; 8499 A; 11500 C; 11073 G; 8830 T; 0 other; tctagagctc gttcctcgac gatcttgctg cgttcggata ttttcgtgga gttcccgcca 60 cagacccgga ttgaaggcga gatccagcaa ctcgcgccag atcatcctgt gacggaactt 120 tggcgcgtga tgactggcca ggacgtcggc cgaaagagcg acaagcagat cacgcttttc 180 gacagcgtcg gatttgcgat cgaggatttt tcggcgctgc gctacgtccg cgaccgcgtt 240 gagggatcaa gccacagcag cccactcgac cttctagccg acccagacga gccaagggat 300 ctttttggaa tgctgctccg tcgtcaggct ttccgacgtt tgggtggttg aacagaagtc 360 attatcgtac ggaatgccaa gcactcccga ggggaaccct gtggttggca tgcacataca 420 aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgttattc taataaacgc 480 tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt ttaaactgaa 540 ggcgggaaac gacaatctga tcatgagcgg agaattaagg gagtcacgtt atgacccccg 600 ccgatgacgc gggacaagcc gttttacgtt tggaactgac agaaccgcaa cgttgaagga 660 gccactcagc ccaagcttga attcgagctc ccctggattt tggttttagg aattagaaat 720 tttattgata gaagtatttt acaaatacaa atacatacta agggtttctt atatgctcaa 780 cacatgagcg aaaccctata agaaccctaa ttcccttatc tgggaactac tcacacatta 840 ttatagagag agatagattt gtagagagag actggtgatt tcagcgggca tgcctgcagg 900 tcgatcgact ctagaggatc cgttgttcgg tcggcatcta ctctattcct ttgccctcgg 960 acgagtgctg gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca 1020 gacggccgcg cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac 1080 gattgcgtcg catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct 1140 ctgatagagt tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc 1200 aagctccgga tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg 1260 cctccagaag aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca 1320 gtcaatgacc gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc 1380 gtgcacgagg tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc 1440 ctgcgcgacg gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg 1500 atcagcaatc gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc 1560 gaatgggccg aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc 1620 cgcgaccggc tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc 1680 ctgtgcacgg cgggagatgc aataggtcag gctctcgctg aattccccaa tgtcaagcac 1740 ttccggaatc gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa 1800 accatcggcg cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa 1860 agcacgagat tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc 1920 gatcagaaac ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc 1980 cccgggatcc ccgggcctgt cctctccaaa tgaaatgaac ttccttatat agaggaaggg 2040 tcttgcgaag gatagtggga ttgtgcgtca tcccttacgt cagtggagat atcacatcaa 2100 tccacttgct ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt 2160 gggggtccat ctttgggacc actgtcggca gaggcatctt caacgatggc ctttccttta 2220 tcgcaatgat ggcatttgta ggagccacct tccttttcca ctatcttcac aataaagtga 2280 cagatagctg ggcaatggaa tccgaggagg tttccggata ttaccctttg ttgaaaagtc 2340 tcaattgccc tttggtcttc tgagactgta tctttgatat ttttggagta gacaagcgtg 2400 tcgtgctcca ccatgttgac gaagattttc ttcttgtcat tgagtcgtaa gagactctgt 2460 atgaactgtt cgccagtctt tacggcgagt tctgttaggt cctctatttg aatctttgac 2520 tccatgggaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc 2580 aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc cgaccggcag 2640 ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg cgtcagcggg 2700 agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg aagaacggca 2760 actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt tgattgtaac 2820 gatgacagag cgttgctgcc tgtgatcaaa tatcatctcc ctcgcagaga tccgaattat 2880 cagccttctt attcatttct cgcttaaccg tgacaggctg tcgatcttga gaactatgcc 2940 gacataatag gaaatcgctg gataaagccg ctgaggaagc tgagtggcgc tatttcttta 3000 gaagtgaacg ttgacgatcg tcgaggaacg gtacctgcgg ggaagcttac aataatgtgt 3060 gttgttaagt cttgttgcct gtcatcgtct gactgacttt cgtcataaat cccggcctcc 3120 gtaacccagc tttgggcaag ctcacggatt tgatccggcg gaacgggaat atcgagatgc 3180 cgggctgaac gctgcagttc cagctttccc tttcgggaca ggtactccag ctgattgatt 3240 atctgctgaa gggtcttggt tccacctcct ggcacaatgc gaatgattac ttgagcgcga 3300 tcgggcatcc aattttctcc cgtcaggtgc gtggtcaagt gctacaaggc acctttcagt 3360 aacgagcgac cgtcgatccg tcgccgggat acggacaaaa tggagcgcag tagtccatcg 3420 agggcggcga aagcctcgcc aaaagcaata cgttcatctc gcacagcctc cagatccgat 3480 cgagggtctt cggcgtaggc agatagaagc atggatacat tgcttgagag tattccgatg 3540 gactgaagta tggcttccat cttttctcgt gtgtctgcat ctatttcgag aaagcccccg 3600 atgcggcgca ccgcaacgcg aattgccata ctatccgaaa gtcccagcag gcgcgcttga 3660 taggaaaagg tttcatactc ggccgatcgc agacgggcac tcacgacctt gaacccttca 3720 actttcaggg atcgatgctg gttgatggta gtctcactcg acgtggctct ggtgtgtttt 3780 gacatagctt cctccaaaga aagcggaagg tctggatact ccagcacgaa atgtgcccgg 3840 gtagacggat ggaagtctag ccctgctcaa tatgaaatca acagtacatt tacagtcaat 3900 actgaatata cttgctacat ttgcaattgt cttataacga atgtgaaata aaaatagtgt 3960 aacaacgctt ttactcatcg ataatcacaa aaacatttat acgaacaaaa atacaaatgc 4020 actccggttt cacaggatag gcgggatcag aatatgcaac ttttgacgtt ttgttctttc 4080 aaagggggtg ctggcaaaac caccgcactc atgggccttt gcgctgcttt ggcaaatgac 4140 ggtaaacgag tggccctctt tgatgccgac gaaaaccggc ctctgacgcg atggagagaa 4200 aacgccttac aaagcagtac tgggatcctc gctgtgaagt ctattccgcc gacgaaatgc 4260 cccttcttga agcagcctat gaaaatgccg agctcgaagg atttgattat gcgttggccg 4320 atacgcgtgg cggctcgagc gagctcaaca acacaatcat cgctagctca aacctgcttc 4380 tgatccccac catgctaacg ccgctcgaca tcgatgaggc actatctacc taccgctacg 4440 tcatcgagct gctgttgagt gaaaatttgg caattcctac agctgttttg cgccaacgcg 4500 tcccggtcgg ccgattgaca acatcgcaac gcaggatgtc agagacgcta gagagccttc 4560 cagttgtacc gtctcccatg catgaaagag atgcatttgc cgcgatgaaa gaacgcggca 4620 tgttgcatct tacattacta aacacgggaa ctgatccgac gatgcgcctc atagagagga 4680 atcttcggat tgcgatggag gaagtcgtgg tcatttcgaa actgatcagc aaaatcttgg 4740 aggcttgaag atggcaattc gcaagcccgc attgtcggtc ggcgaagcac ggcggcttgc 4800 tggtgctcga cccgagatcc accatcccaa cccgacactt gttccccaga agctggacct 4860 ccagcacttg cctgaaaaag ccgacgagaa agaccagcaa cgtgagcctc tcgtcgccga 4920 tcacatttac agtcccgatc gacaacttaa gctaactgtg gatgccctta gtccacctcc 4980 gtccccgaaa aagctccagg tttttctttc agcgcgaccg cccgcgcctc aagtgtcgaa 5040 aacatatgac aacctcgttc ggcaatacag tccctcgaag tcgctacaaa tgattttaag 5100 gcgcgcgttg gacgatttcg aaagcatgct ggcagatgga tcatttcgcg tggccccgaa 5160 aagttatccg atcccttcaa ctacagaaaa atccgttctc gttcagacct cacgcatgtt 5220 cccggttgcg ttgctcgagg tcgctcgaag tcattttgat ccgttggggt tggagaccgc 5280 tcgagctttc ggccacaagc tggctaccgc cgcgctcgcg tcattctttg ctggagagaa 5340 gccatcgagc aattggtgaa gagggaccta tcggaacccc tcaccaaata ttgagtgtag 5400 gtttgaggcc gctggccgcg tcctcagtca ccttttgagc cagataatta agagccaaat 5460 gcaattggct caggctgcca tcgtcccccc gtgcgaaacc tgcacgtccg cgtcaaagaa 5520 ataaccggca cctcttgctg tttttatcag ttgagggctt gacggatccg cctcaagttt 5580 gcggcgcagc cgcaaaatga gaacatctat actcctgtcg taaacctcct cgtcgcgtac 5640 tcgactggca atgagaagtt gctcgcgcga tagaacgtcg cggggtttct ctaaaaacgc 5700 gaggagaaga ttgaactcac ctgccgtaag tttcacctca ccgccagctt cggacatcaa 5760 gcgacgttgc ctgagattaa gtgtccagtc agtaaaacaa aaagaccgtc ggtctttgga 5820 gcggacaacg ttggggcgca cgcgcaaggc aacccgaatg cgtgcaagaa actctctcgt 5880 actaaacggc ttagcgataa aatcacttgc tcctagctcg agtgcaacaa ctttatccgt 5940 ctcctcaagg cggtcgccac tgataattat gattggaata tcagactttg ccgccagatt 6000 tcgaacgatc tcaagcccat cttcacgacc taaatttaga tcaacaacca cgacatcgac 6060 cgtcgcggaa gagagtactc tagtgaactg ggtgctgtcg gctaccgcgg tcactttgaa 6120 ggcgtggatc gtaaggtatt cgataataag atgccgcata gcgacatcgt catcgataag 6180 aagaacgtgt ttcaacggct cacctttcaa tctaaaatct gaacccttgt tcacagcgct 6240 tgagaaattt tcacgtgaag gatgtacaat catctccagc taaatgggca gttcgtcaga 6300 attgcggctg accgcggatg acgaaaatgc gaaccaagta tttcaatttt atgacaaaag 6360 ttctcaatcg ttgttacaag tgaaacgctt cgaggttaca gctactattg attaaggaga 6420 tcgcctatgg tctcgccccg gcgtcgtgcg tccgccgcga gccagatctc gcctacttca 6480 taaacgtcct cataggcacg gaatggaatg atgacatcga tcgccgtaga gagcatgtca 6540 atcagtgtgc gatcttccaa gctagcacct tgggcgctac ttttgacaag ggaaaacagt 6600 ttcttgaatc cttggattgg attcgcgccg tgtattgttg aaatcgatcc cggatgtccc 6660 gagacgactt cactcagata agcccatgct gcatcgtcgc gcatctcgcc aagcaatatc 6720 cggtccggcc gcatacgcag acttgcttgg agcaagtgct cggcgctcac agcacccagc 6780 ccagcaccgt tcttggagta gagtagtcta acatgattat cgtgtggaat gacgagttcg 6840 agcgtatctt ctatggtgat tagcctttcc tgggggggga tggcgctgat caaggtcttg 6900 ctcattgttg tcttgccgct tccggtaggg ccacatagca acatcgtcag tcggctgacg 6960 acgcatgcgt gcagaaacgc ttccaaatcc ccgttgtcaa aatgctgaag gatagcttca 7020 tcatcctgat tttggcgttt ccttcgtgtc tgccactggt tccacctcga agcatcataa 7080 cgggaggaga cttctttaag accagaaaca cgcgagcttg gccgtcgaat ggtcaagctg 7140 acggtgcccg agggaacggt cggcggcaga cagatttgta gtcgttcacc accaggaagt 7200 tcagtggcgc agagggggtt acgtggtccg acatcctgct ttctcagcgc gcccgctaaa 7260 atagcgatat cttcaagatc atcataagag acgggcaaag gcatcttggt aaaaatgccg 7320 gcttggcgca caaatgcctc tccaggtcga ttgatcgcaa tttcttcagt cttcgggtca 7380 tcgagccatt ccaaaatcgg cttcagaaga aagcgtagtt gcggatccac ttccatttac 7440 aatgtatcct atctctaagc ggaaatttga attcattaag agcggcggtt cctcccccgc 7500 gtggcgccgc cagtcaggcg gagctggtaa acaccaaaga aatcgaggtc ccgtgctacg 7560 aaaatggaaa cggtgtcacc ctgattcttc ttcagggttg gcggtatgtt gatggttgcc 7620 ttaagggctg tctcagttgt ctgctcaccg ttattttgaa agctgttgaa gctcatcccg 7680 ccacccgagc tgccggcgta ggtgctagct gcctggaagg cgccttgaac aacactcaag 7740 agcatagctc cgctaaaacg ctgccagaag tggctgtcga ccgagcccgg caatcctgag 7800 cgaccgagtt cgtccgcgct tggcgatgtt aacgagatca tcgcatggtc aggtgtctcg 7860 gcgcgatccc acaacacaaa aacgcgccca tctccctgtt gcaagccacg ctgtatttcg 7920 ccaacaacgg tggtgccacg atcaagaagc acgatattgt tcgttgttcc acgaatatcc 7980 tgaggcaaga cacactttac atagcctgcc aaatttgtgt cgattgcggt ttgcaagatg 8040 cacggaatta ttgtcccttg cgttaccata aaatcggggt gcggcaagag cgtggcgctg 8100 ctgggctgca gctcggtggg tttcatacgt atcgacaaat cgttctcgcc ggacacttcg 8160 ccattcggca aggagttgtc gtcacgcttg ccttcttgtc ttcggcccgt gtcgccctga 8220 atggcgcgtt tgctgacccc ttgatcgccg ctgctatatg caaaaatcgg tgtttcttcc 8280 ggccgtggct catgccgctc cggttcgccc ctcggcggta gaggagcagc aggctgaaca 8340 gcctcttgaa ccgctggagg atccggcggc acctcaatcg gagctggatg aaatggcttg 8400 gtgtttgttg cgatcaaagt tgacggcgat gcgttctcat tcaccttctt ttggcgccca 8460 cctagccaaa tgaggcttaa tgataacgcg agaacgacac ctccgacgat caatttctga 8520 gaccccgaaa gacgccggcg atgtttgtcg gagaccaggg atccagatgc atcaacctca 8580 tgtgccgctt gctgactatc gttattcatc ccttcgcccc cttcaggacg cgtttcacat 8640 cgggcctcac cgtgcccgtt tgcggccttt ggccaacggg atcgtaagcg gtgttccaga 8700 tacatagtac tgtgtggcca tccctcagac gccaacctcg ggaaaccgaa gaaatctcga 8760 catcgctccc tttaactgaa tagttggcaa cagcttcctt gccatcagga ttgatggtgt 8820 agatggaggg tatgcgtaca ttgcccggaa agtggaatac cgtcgtaaat ccattgtcga 8880 agacttcgag tggcaacagc gaacgatcgc cttgggcgac gtagtgccaa ttactgtccg 8940 ccgcaccaag ggctgtgaca ggctgatcca ataaattctc agctttccgt tgatattgtg 9000 cttccgcgtg tagtctgtcc acaacagcct tctgttgtgc ctcccttcgc cgagccgccg 9060 catcgtcggc ggggtaggcg aattggacgc tgtaatagag atcgggctgc tctttatcga 9120 ggtgggacag agtcttggaa cttatactga aaacataacg gcgcatcccg gagtcgcttg 9180 cggttagcac gattactggc tgaggcgtga ggacctggct tgccttgaaa aatagataat 9240 ttccccgcgg tagggctgct agatctttgc tatttgaaac ggcaaccgct gtcaccgttt 9300 cgttcgtggc gaatgttacg accaaagtag ctccaaccgc cgtcgagagg cgcaccactt 9360 gatcgggatt gtaagccaaa taacgcatgc gcggatctag cttgcccgcc attggagtgt 9420 cttcagcctc cgcaccagtc gcagcggcaa ataaacatgc taaaatgaaa agtgcttttc 9480 tgatcatggt tcgctgtggc ctacgtttga aacggtatct tccgatgtct gataggaggt 9540 gacaaccaga cctgccgggt tggttagtct caatctgccg ggcaagctgg tcaccttttc 9600 gtagcgaact gtcgcggtcc acgtactcac cacaggcatt ttgccgtcaa cgacgagggt 9660 ccttttatag cgaatttgct gcgtgcttgg agttacatca tttgaagcga tgtgctcgac 9720 ctccaccctg ccgcgtttgc caagaatgac ttgaggcgaa ctgggattgg gatagttgaa 9780 gaattgctgg taatcctggc gcactgttgg ggcactgaag ttcgatacca ggtcgtaggc 9840 gtactgagcg gtgtcggcat cataactctc gcgcaggcga acgtactccc acaatgaggc 9900 gttaacgacg gcctcctctt gagttgcagg caatcgcgag acagacacct cgctgtcaac 9960 ggtgccgtcc ggccgtatcc atagatatac gggcacaagc ctgctcaacg gcaccattgt 10020 ggctatagcg aacgcttgag caacatttcc caaaatcgcg atagctgcga cagctgcaat 10080 gagtttggag agacgtcgcg ccgatttcgc tcgcgcggtt tgaaaggctt ctacttcctt 10140 atagtgctcg gcaaggcttt cgcgcgccac tagcatggca tattcaggcc ccgtcatagc 10200 gtccacccga attgccgagc tgaagatctg acggagtagg ctgccatcgc cccacattca 10260 gcgggaagat cgggcctttg cagctcgcta atgtgtcgtt tgtctggcag ccgctcaaag 10320 cgacaactag gcacagcagg caatacttca tagaattctc cattgaggcg aatttttgcg 10380 cgacctagcc tcgctcaacc tgagcgaagc gacggtacaa gctgctggca gattgggttg 10440 cgccgctcca gtaactgcct ccaatgttgc cggcgatcgc cggcaaagcg acaatgagcg 10500 catcccctgt cagaaaaaac atatcgagtt cgtaaagacc aatgatcttg gccgcggtcg 10560 taccggcgaa ggtgattaca ccaagcataa gggtgagcgc agtcgcttcg gttaggatga 10620 cgatcgttgc cacgaggttt aagaggagaa gcaagagacc gtaggtgata agttgcccga 10680 tccacttagc tgcgatgtcc cgcgtgcgat caaaaatata tccgacgagg atcagaggcc 10740 cgatcgcgag aagcactttc gtgagaattc caacggcgtc gtaaactccg aaggcagacc 10800 agagcgtgcc gtaaaggacc cactgtgccc cttggaaagc aaggatgtcc tggtcgttca 10860 tcggaccgat ttcggatgcg attttctgaa aaacggcctg ggtcacggcg aacattgtat 10920 ccaactgtgc cggaacagtc tgcagaggca agccggttac actaaactgc tgaacaaagt 10980 ttgggaccgt cttttcgaag atggaaacca catagtcttg gtagttagcc tgcccaacaa 11040 ttagagcaac aacgatggtg accgtgatca cccgagtgat accgctacgg gtatcgactt 11100 cgccgcgtat gactaaaata ccctgaacaa taatccaaag agtgacacag gcgatcaatg 11160 gcgcactcac cgcctcctgg atagtctcaa gcatcgagtc caagcctgtc gtgaaggcta 11220 catcgaagat cgtatgaatg gccgtaaacg gcgccggaat cgtgaaattc atcgattgga 11280 cctgaacttg actggtttgt cgcataatgt tggataaaat gagctcgcat tcggcgagga 11340 tgcgggcgga tgaacaaatc gcccagcctt aggggagggc accaaagatg acagcggtct 11400 tttgatgctc cttgcgttga gcggccgcct cttccgcctc gtgaaggccg gcctgcgcgg 11460 tagtcatcgt taataggctt gtcgcctgta cattttgaat cattgcgtca tggatctgct 11520 tgagaagcaa accattggtc acggttgcct gcatgatatt gcgagatcgg gaaagctgag 11580 cagacgtatc agcattcgcc gtcaagcgtt tgtccatcgt ttccagattg tcagccgcaa 11640 tgccagcgct gtttgcggaa ccggtgatct gcgatcgcaa caggtccgct tcagcatcac 11700 tacccacgac tgcacgatct gtatcgctgg tgatcgcacg tgccgtggtc gacattggca 11760 ttcgcggcga aaacatttca ttgtctaggt ccttcgtcga aggatactga tttttctggt 11820 tgagcgaagt cagtagtcca gtaacgccgt aggccgacgt caacatcgta accatcgcta 11880 tagtctgagt gagattctcc gcagtcgcga gcgcagtcgc gagcgtctca gcctccgttg 11940 ccgggtcgct aacaacaaac tgcgcccgcg cgggctgaat atatagaaag ctgcaggtca 12000 aaactgttgc aataagttgc gtcgtcttca tcgtttccta ccttatcaat cttctgcctc 12060 gtggtgacgg gccatgaatt cgctgagcca gccagatgag ttgccttctt gtgcctcgcg 12120 tagtcgagtt gcaaagcgca ccgtgttggc acgccccgaa agcacggcga catattcacg 12180 catatcccgc agatcaaatt cgcagatgac gcttccactt tctcgtttaa gaagaaactt 12240 acggctgccg accgtcatgt cttcacggat cgcctgaaat tccttttcgg tacatttcag 12300 tccatcgaca taagccgatc gatctgcggt tggtgatgga tagaaaatct tcgtcataca 12360 ttgcgcaacc aagctggctc ctagcggcga ttccagaaca tgctctggtt gctgcgttgc 12420 cagtattagc atcccgttgt tttttcgaac ggtcaggagg aatttgtcga cgacagtcga 12480 aaatttaggg tttaacaaat aggcgcgaaa ctcatcgcag ctcatcacaa aacggcggcc 12540 gtcgatcatg gctccaatcc gatgcaggag atatgctgca gcgggagcgc atacttcctc 12600 gtattcgaga agatgcgtca tgtcgaagcc ggtaatcgac ggatctaact ttacttcgtc 12660 aacttcgccg tcaaatgccc agccaagcgc atggccccgg caccagcgtt ggagccgcgc 12720 tcctgcgcct tcggcgggcc catgcaacaa aaattcacgt aaccccgcga ttgaacgcat 12780 ttgtggatca aacgagagct gacgatggat accacggacc agacggcggt tctcttccgg 12840 agaaatccca ccccgaccat cactctcgat gagagccacg atccattcgc gcagaaaatc 12900 gtgtgaggct gctgtgtttt ctaggccacg caacggcgcc aacccgctgg gtgtgcctct 12960 gtgaagtgcc aaatatgttc ctcctgtggc gcgaaccagc aattcgccac cccggtcctt 13020 gtcaaagaac acgaccgtac ctgcacggtc gaccatgctc tgttcgagca tggctagaac 13080 aaacatcatg agcgtcgtct tacccctccc gataggcccg aatattgccg tcatgccaac 13140 atcgtgctca tgcgggatat agtcgaaagg cgttccgcca ttggtacgaa atcgggcaat 13200 cgcgttgccc cagtggcctg agctggcgcc ctctggaaag ttttcgaaag agacaaaccc 13260 tgcgaaattg cgtgaagtga ttgcgccagg gcgtgtgcgc cacttaaaat tccccggcaa 13320 ttgggaccaa taggccgctt ccataccaat accttcttgg acaaccacgg cacctgcatc 13380 cgccattcgt gtccgagccc gcgcgcccct gtccccaaga ctattgagat cgtctgcata 13440 gacgcaaagg ctcaaatgat gtgagcccat aacgaattcg ttgctcgcaa gtgcgtcctc 13500 agcctcggat aatttgccga tttgagtcac ggctttatcg ccggaactca gcatctggct 13560 cgatttgagg ctaagtttcg cgtgcgcttg cgggcgagtc aggaacgaaa aactctgcgt 13620 gagaacaagt ggaaaatcga gggatagcag cgcgttgagc atgcccggcc gtgtttttgc 13680 agggtattcg cgaaacgaat agatggatcc aacgtaactg tcttttggcg ttctgatctc 13740 gagtcctcgc ttgccgcaaa tgactctgtc ggtataaatc gaagcgccga gtgagccgct 13800 gacgaccgga accggtgtga accgaccagt catgatcaac cgtagcgctt cgccaatttc 13860 ggtgaagagc acaccctgct tctcgcggat gccaagacga tgcaggccat acgctttaag 13920 agagccagcg acaacatgcc aaagatcttc catgttcctg atctggcccg tgagatcgtt 13980 ttcccttttt ccgcttagct tggtgaacct cctctttacc ttccctaaag ccgcctgtgg 14040 gtagacaatc aacgtaagga agtgttcatt gcggaggagt tggccggaga gcacgcgctg 14100 ttcaaaagct tcgttcaggc tagcggcgaa aacactacgg aagtgtcgcg gcgccgatga 14160 tggcacgtcg gcatgacgta cgaggtgagc atatattgac acatgatcat cagcgatatt 14220 gcgcaacagc gtgttgaacg cacgacaacg cgcattgcgc atttcagttt cctcaagctc 14280 gaatgcaacg ccatcaattc tcgcaatggt catgatcgat ccgtcttcaa gaaggacgat 14340 atggtcgctg aggtggccaa tataagggag atagatctca ccggatcttt cggtcgttcc 14400 actcgcgccg agcatcacac cattcctctc cctcgtgggg gaaccctaat tggatttggg 14460 ctaacagtag cgccccccca aactgcacta tcaatgcttc ttcccgcggt ccgcaaaaat 14520 agcaggacga cgctcgccgc attgtagtct cgctccacga tgagccgggc tgcaaaccat 14580 aacggcacga gaacgacttc gtagagcggg ttctgaacga taacgatgac aaagccggcg 14640 aacatcatga ataaccctgc caatgtcagt ggcaccccaa gaaacaatgc gggccgtgtg 14700 gctgcgaggt aaagggtcga ttcttccaaa cgatcagcca tcaactaccg ccagtgagcg 14760 tttggccgag gaagctcgcc ccaaacatga taacaatgcc gccgacgacg ccggcaacca 14820 gcccaagcga agcccgcccg aacatccagg agatcccgat agcgacaatg ccgagaacag 14880 cgagtgactg gccgaacgga ccaaggataa acgtgcatat attgttaacc attgtggcgg 14940 ggtcagtgcc gccacccgca gattgcgctg cggcgggtcc ggatgaggaa atgctccatg 15000 caattgcacc gcacaagctt ggggcgcagc tcgatatcac gcgcatcatc gcattcgaga 15060 gcgagaggcg atttagatgt aaacggtatc tctcaaagca tcgcatcaat gcgcacctcc 15120 ttagtataag tcgaataaga cttgattgtc gtctgcggat ttgccgttgt cctggtgtgg 15180 cggtggcgga gcgattaaac cgccagcgcc atcctcctgc gagcggcgct gatatgaccc 15240 ccaaacatcc cacgtctctt cggattttag cgcctcgtga tcgtcttttg gaggctcgat 15300 taacgcgggc accagcgatt gagcagctgt ttcaactttt cgcacgtagc cgtttgcaaa 15360 accgccgatg aaattaccgg tgttgtaagc ggagatcgcc cgacgaagcg caaattgctt 15420 ctcgtcaatc gtttcgccgc ctgcataacg acttttcagc atgtttgcag cggcagataa 15480 tgatgtgcac gcctggagcg caccgtcagg tgtcagaccg agcatagaaa aatttcgaga 15540 gtttatttgc atgaggccaa catccagcga atgccgtgca tcgagacggt gcctgacgac 15600 ttgggttgct tggctgtgat cttgccagtg aagcgtttcg ccggtcgtgt tgtcatgaat 15660 cgctaaagga tcaaagcgac tctccacctt agctatcgcc gcaagcgtag atgtcgcaac 15720 tgatggggca cacttgcgag caacatggtc aaactcagca gatgagagtg gcgtggcaag 15780 gctcgacgaa cagaaggaga ccatcaaggc aagagaaagc gaccccgatc tcttaagcat 15840 accttatctc cttagctcgc aactaacacc gcctctcccg ttggaagaag tgcgttgttt 15900 tatgttgaag attatcggga gggtcggtta ctcgaaaatt ttcaattgct tctttatgat 15960 ttcaattgaa gcgagaaacc tcgcccggcg tcttggaacg caacatggac cgagaaccgc 16020 gcatccatga ctaagcaacc ggatcgacct attcaggccg cagttggtca ggtcaggctc 16080 agaacgaaaa tgctcggcga ggttacgctg tctgtaaacc cattcgatga acgggaagct 16140 tccttccgat tgctcttggc aggaatattg gcccatgcct gcttgcgctt tgcaaatgct 16200 cttatcgcgt tggtatcata tgccttgtcc gccagcagaa acgcactcta agcgattatt 16260 tgtaaaaatg tttcggtcat gcggcggtca tgggcttgac ccgctgtcag cgcaagacgg 16320 atcggtcaac cgtcggcatc gacaacagcg tgaatcttgg tggtcaaacc gccacgggaa 16380 cgtcccatac agccatcgtc ttgatcccgc tgtttcccgt cgccgcatgt tggtggacgc 16440 ggacacagga actgtcaatc atgacgacat tctatcgaaa gccttggaaa tcacactcag 16500 aatatgatcc cagacgtctg cctcacgcca tcgtacaaag cgattgtagc aggttgtaca 16560 ggaaccgtat cgatcaggaa cgtctgccca gggcgggccc gtccggaagc gccacaagat 16620 gacattgatc acccgcgtca acgcgcggca cgcgacgcgg cttatttggg aacaaaggac 16680 tgaacaacag tccattcgaa atcggtgaca tcaaagcggg gacgggttat cagtggcctc 16740 caagtcaagc ctcaatgaat caaaatcaga ccgatttgca aacctgattt atgagtgtgc 16800 ggcctaaatg atgaaatcgt ccttctagat cgcctccgtg gtgtagcaac acctcgcagt 16860 atcgccgtgc tgaccttggc cagggaattg actggcaagg gtgctttcac atgaccgctc 16920 ttttggccgc gatagatgat ttcgttgctg ctttgggcac gtagaaggag agaagtcata 16980 tcggagaaat tcctcctggc gcgagagcct gctctatcgc gacggcatcc cactgtcggg 17040 aacagaccgg atcattcacg aggcgaaagt cgtcaacaca tgcgttatag gcatcttccc 17100 ttgaaggatg atcttgttgc tgccaatctg gaggtgcggc agccgcaggc agatgcgatc 17160 tcagcgcaac ttgcggcaaa acatctcact cacctgaaaa ccactagcga gtctcgcgat 17220 cagacgaagg ccttttactt aacgacacaa tatccgatgt ctgcatcaca ggcgtcgcta 17280 tcccagtcaa tactaaagcg gtgcaggaac taaagattac tgatgactta ggcgtgccac 17340 gaggcctgag acgacgcgcg tagacagttt tttgaaatca ttatcaaagt gatggcctcc 17400 gctgaagcct atcacctctg cgccggtctg tcggagagat gggcaagcat tattacggtc 17460 ttcgcgcccg tacatgcatt ggacgattgc agggtcaatg gatctgagat catccagagg 17520 attgccgccc ttaccttccg tttcgagttg gagccagccc ctaaatgaga cgacatagtc 17580 gacttgatgt gacaatgcca agagagagat ttgcttaacc cgattttttt gctcaagcgt 17640 aagcctattg aagcttgccg gcatgacgtc cgcgccgaaa gaatatccta caagtaaaac 17700 attctgcaca ccgaaatgct tggtgtagac atcgattatg tgaccaagat ccttagcagt 17760 ttcgcttggg gaccgctccg accagaaata ccgaagtgaa ctgacgccaa tgacaggaat 17820 cccttccgtc tgcagatagg taccatcgat agatctgctg cctcgcgcgt ttcggtgatg 17880 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 17940 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 18000 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc 18060 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 18120 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 18180 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 18240 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 18300 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 18360 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 18420 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 18480 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 18540 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 18600 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 18660 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 18720 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 18780 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 18840 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 18900 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 18960 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 19020 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 19080 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 19140 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 19200 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 19260 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 19320 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 19380 caacgttgtt gccattgctg cagggggggg gggggggggg gacttccatt gttcattcca 19440 cggacaaaaa cagagaaagg aaacgacaga ggccaaaaag cctcgctttc agcacctgtc 19500 gtttcctttc ttttcagagg gtattttaaa taaaaacatt aagttatgac gaagaagaac 19560 ggaaacgcct taaaccggaa aattttcata aatagcgaaa acccgcgagg tcgccgcccc 19620 gtaacctgtc ggatcaccgg aaaggacccg taaagtgata atgattatca tctacatatc 19680 acaacgtgcg tggaggccat caaaccacgt caaataatca attatgacgc aggtatcgta 19740 ttaattgatc tgcatcaact taacgtaaaa acaacttcag acaatacaaa tcagcgacac 19800 tgaatacggg gcaacctcat gtcccccccc cccccccccc tgcaggcatc gtggtgtcac 19860 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 19920 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 19980 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 20040 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 20100 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc 20160 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 20220 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 20280 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 20340 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 20400 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 20460 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 20520 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 20580 ttcgtcttca agaattcgga gcttttgcca ttctcaccgg attcagtcgt cactcatggt 20640 gatttctcac ttgataacct tatttttgac gaggggaaat taataggttg tattgatgtt 20700 ggacgagtcg gaatcgcaga ccgataccag gatcttgcca tcctatggaa ctgcctcggt 20760 gagttttctc cttcattaca gaaacggctt tttcaaaaat atggtattga taatcctgat 20820 atgaataaat tgcagtttca tttgatgctc gatgagtttt tctaatcaga attggttaat 20880 tggttgtaac actggcagag cattacgctg acttgacggg acggcggctt tgttgaataa 20940 atcgaacttt tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt 21000 ccgtggcaaa gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca 21060 accgtggctc cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag 21120 caacaccttc ttcacgaggc agacctcagc gccagaaggc cgccagagag gccgagcgcg 21180 gccgtgaggc ttggacgcta gggcagggca tgaaaaagcc cgtagcgggc tgctacgggc 21240 gtctgacgcg gtggaaaggg ggaggggatg ttgtctacat ggctctgctg tagtgagtgg 21300 gttgcgctcc ggcagcggtc ctgatcaatc gtcacccttt ctcggtcctt caacgttcct 21360 gacaacgagc ctccttttcg ccaatccatc gacaatcacc gcgagtccct gctcgaacgc 21420 tgcgtccgga ccggcttcgt cgaaggcgtc tatcgcggcc cgcaacagcg gcgagagcgg 21480 agcctgttca acggtgccgc cgcgctcgcc ggcatcgctg tcgccggcct gctcctcaag 21540 cacggcccca acagtgaagt agctgattgt catcagcgca ttgacggcgt ccccggccga 21600 aaaacccgcc tcgcagagga agcgaagctg cgcgtcggcc gtttccatct gcggtgcgcc 21660 cggtcgcgtg ccggcatgga tgcgcgcgcc atcgcggtag gcgagcagcg cctgcctgaa 21720 gctgcgggca ttcccgatca gaaatgagcg ccagtcgtcg tcggctctcg gcaccgaatg 21780 cgtatgattc tccgccagca tggcttcggc cagtgcgtcg agcagcgccc gcttgttcct 21840 gaagtgccag taaagcgccg gctgctgaac ccccaaccgt tccgccagtt tgcgtgtcgt 21900 cagaccgtct acgccgacct cgttcaacag gtccagggcg gcacggatca ctgtattcgg 21960 ctgcaacttt gtcatgcttg acactttatc actgataaac ataatatgtc caccaactta 22020 tcagtgataa agaatccgcg cgttcaatcg gaccagcgga ggctggtccg gaggccagac 22080 gtgaaaccca acatacccct gatcgtaatt ctgagcactg tcgcgctcga cgctgtcggc 22140 atcggcctga ttatgccggt gctgccgggc ctcctgcgcg atctggttca ctcgaacgac 22200 gtcaccgccc actatggcat tctgctggcg ctgtatgcgt tggtgcaatt tgcctgcgca 22260 cctgtgctgg gcgcgctgtc ggatcgtttc gggcggcggc caatcttgct cgtctcgctg 22320 gccggcgcca ctgtcgacta cgccatcatg gcgacagcgc ctttcctttg ggttctctat 22380 atcgggcgga tcgtggccgg catcaccggg gcgactgggg cggtagccgg cgcttatatt 22440 gccgatatca ctgatggcga tgagcgcgcg cggcacttcg gcttcatgag cgcctgtttc 22500 gggttcggga tggtcgcggg acctgtgctc ggtgggctga tgggcggttt ctccccccac 22560 gctccgttct tcgccgcggc agccttgaac ggcctcaatt tcctgacggg ctgtttcctt 22620 ttgccggagt cgcacaaagg cgaacgccgg ccgttacgcc gggaggctct caacccgctc 22680 gcttcgttcc ggtgggcccg gggcatgacc gtcgtcgccg ccctgatggc ggtcttcttc 22740 atcatgcaac ttgtcggaca ggtgccggcc gcgctttggg tcattttcgg cgaggatcgc 22800 tttcactggg acgcgaccac gatcggcatt tcgcttgccg catttggcat tctgcattca 22860 ctcgcccagg caatgatcac cggccctgta gccgcccggc tcggcgaaag gcgggcactc 22920 atgctcggaa tgattgccga cggcacaggc tacatcctgc ttgccttcgc gacacgggga 22980 tggatggcgt tcccgatcat ggtcctgctt gcttcgggtg gcatcggaat gccggcgctg 23040 caagcaatgt tgtccaggca ggtggatgag gaacgtcagg ggcagctgca aggctcactg 23100 gcggcgctca ccagcctgac ctcgatcgtc ggacccctcc tcttcacggc gatctatgcg 23160 gcttctataa caacgtggaa cgggtgggca tggattgcag gcgctgccct ctacttgctc 23220 tgcctgccgg cgctgcgtcg cgggctttgg agcggcgcag ggcaacgagc cgatcgctga 23280 tcgtggaaac gataggccta tgccatgcgg gtcaaggcga cttccggcaa gctatacgcg 23340 ccctaggagt gcggttggaa cgttggccca gccagatact cccgatcacg agcaggacgc 23400 cgatgatttg aagcgcactc agcgtctgat ccaagaacaa ccatcctagc aacacggcgg 23460 tccccgggct gagaaagccc agtaaggaaa caactgtagg ttcgagtcgc gagatccccc 23520 ggaaccaaag gaagtaggtt aaacccgctc cgatcaggcc gagccacgcc aggccgagaa 23580 cattggttcc tgtaggcatc gggattggcg gatcaaacac taaagctact ggaacgagca 23640 gaagtcctcc ggccgccagt tgccaggcgg taaaggtgag cagaggcacg ggaggttgcc 23700 acttgcgggt cagcacggtt ccgaacgcca tggaaaccgc ccccgccagg cccgctgcga 23760 cgccgacagg atctagcgct gcgtttggtg tcaacaccaa cagcgccacg cccgcagttc 23820 cgcaaatagc ccccaggacc gccatcaatc gtatcgggct acctagcaga gcggcagaga 23880 tgaacacgac catcagcggc tgcacagcgc ctaccgtcgc cgcgaccccg cccggcaggc 23940 ggtagaccga aataaacaac aagctccaga atagcgaaat attaagtgcg ccgaggatga 24000 agatgcgcat ccaccagatt cccgttggaa tctgtcggac gatcatcacg agcaataaac 24060 ccgccggcaa cgcccgcagc agcataccgg cgacccctcg gcctcgctgt tcgggctcca 24120 cgaaaacgcc ggacagatgc gccttgtgag cgtccttggg gccgtcctcc tgtttgaaga 24180 ccgacagccc aatgatctcg ccgtcgatgt aggcgccgaa tgccacggca tctcgcaacc 24240 gttcagcgaa cgcctccatg ggctttttct cctcgtgctc gtaaacggac ccgaacatct 24300 ctggagcttt cttcagggcc gacaatcgga tctcgcggaa atcctgcacg tcggccgctc 24360 caagccgtcg aatctgagcc ttaatcacaa ttgtcaattt taatcctctg tttatcggca 24420 gttcgtagag cgcgccgtgc gtcccgagcg atactgagcg aagcaagtgc gtcgagcagt 24480 gcccgcttgt tcctgaaatg ccagtaaagc gctggctgct gaacccccag ccggaactga 24540 ccccacaagg ccctagcgtt tgcaatgcac caggtcatca ttgacccagg cgtgttccac 24600 caggccgctg cctcgcaact cttcgcaggc ttcgccgacc tgctcgcgcc acttcttcac 24660 gcgggtggaa tccgatccgc acatgaggcg gaaggtttcc agcttgagcg ggtacggctc 24720 ccggtgcgag ctgaaatagt cgaacatccg tcgggccgtc ggcgacagct tgcggtactt 24780 ctcccatatg aatttcgtgt agtggtcgcc agcaaacagc acgacgattt cctcgtcgat 24840 caggacctgg caacgggacg ttttcttgcc acggtccagg acgcggaagc ggtgcagcag 24900 cgacaccgat tccaggtgcc caacgcggtc ggacgtgaag cccatcgccg tcgcctgtag 24960 gcgcgacagg cattcctcgg ccttcgtgta ataccggcca ttgatcgacc agcccaggtc 25020 ctggcaaagc tcgtagaacg tgaaggtgat cggctcgccg ataggggtgc gcttcgcgta 25080 ctccaacacc tgctgccaca ccagttcgtc atcgtcggcc cgcagctcga cgccggtgta 25140 ggtgatcttc acgtccttgt tgacgtggaa aatgaccttg ttttgcagcg cctcgcgcgg 25200 gattttcttg ttgcgcgtgg tgaacagggc agagcgggcc gtgtcgtttg gcatcgctcg 25260 catcgtgtcc ggccacggcg caatatcgaa caaggaaagc tgcatttcct tgatctgctg 25320 cttcgtgtgt ttcagcaacg cggcctgctt ggcctcgctg acctgttttg ccaggtcctc 25380 gccggcggtt tttcgcttct tggtcgtcat agttcctcgc gtgtcgatgg tcatcgactt 25440 cgccaaacct gccgcctcct gttcgagacg acgcgaacgc tccacggcgg ccgatggcgc 25500 gggcagggca gggggagcca gttgcacgct gtcgcgctcg atcttggccg tagcttgctg 25560 gaccatcgag ccgacggact ggaaggtttc gcggggcgca cgcatgacgg tgcggcttgc 25620 gatggtttcg gcatcctcgg cggaaaaccc cgcgtcgatc agttcttgcc tgtatgcctt 25680 ccggtcaaac gtccgattca ttcaccctcc ttgcgggatt gccccgactc acgccggggc 25740 aatgtgccct tattcctgat ttgacccgcc tggtgccttg gtgtccagat aatccacctt 25800 atcggcaatg aagtcggtcc cgtagaccgt ctggccgtcc ttctcgtact tggtattccg 25860 aatcttgccc tgcacgaata ccagcgaccc cttgcccaaa tacttgccgt gggcctcggc 25920 ctgagagcca aaacacttga tgcggaagaa gtcggtgcgc tcctgcttgt cgccggcatc 25980 gttgcgccac tcttcattaa ccgctatatc gaaaattgct tgcggcttgt tagaattgcc 26040 atgacgtacc tcggtgtcac gggtaagatt accgataaac tggaactgat tatggctcat 26100 atcgaaagtc tccttgagaa aggagactct agtttagcta aacattggtt ccgctgtcaa 26160 gaactttagc ggctaaaatt ttgcgggccg cgaccaaagg tgcgaggggc ggcttccgct 26220 gtgtacaacc agatattttt caccaacatc cttcgtctgc tcgatgagcg gggcatgacg 26280 aaacatgagc tgtcggagag ggcaggggtt tcaatttcgt ttttatcaga cttaaccaac 26340 ggtaaggcca acccctcgtt gaaggtgatg gaggccattg ccgacgccct ggaaactccc 26400 ctacctcttc tcctggagtc caccgacctt gaccgcgagg cactcgcgga gattgcgggt 26460 catcctttca agagcagcgt gccgcccgga tacgaacgca tcagtgtggt tttgccgtca 26520 cataaggcgt ttatcgtaaa gaaatggggc gacgacaccc gaaaaaagct gcgtggaagg 26580 ctctgacgcc aagggttagg gcttgcactt ccttctttag ccgctaaaac ggccccttct 26640 ctgcgggccg tcggctcgcg catcatatcg acatcctcaa cggaagccgt gccgcgaatg 26700 gcatcgggcg ggtgcgcttt gacagttgtt ttctatcaga acccctacgt cgtgcggttc 26760 gattagctgt ttgtcttgca ggctaaacac tttcggtata tcgtttgcct gtgcgataat 26820 gttgctaatg atttgttgcg taggggttac tgaaaagtga gcgggaaaga agagtttcag 26880 accatcaagg agcgggccaa gcgcaagctg gaacgcgaca tgggtgcgga cctgttggcc 26940 gcgctcaacg acccgaaaac cgttgaagtc atgctcaacg cggacggcaa ggtgtggcac 27000 gaacgccttg gcgagccgat gcggtacatc tgcgacatgc ggcccagcca gtcgcaggcg 27060 attatagaaa cggtggccgg attccacggc aaagaggtca cgcggcattc gcccatcctg 27120 gaaggcgagt tccccttgga tggcagccgc tttgccggcc aattgccgcc ggtcgtggcc 27180 gcgccaacct ttgcgatccg caagcgcgcg gtcgccatct tcacgctgga acagtacgtc 27240 gaggcgggca tcatgacccg cgagcaatac gaggtcatta aaagcgccgt cgcggcgcat 27300 cgaaacatcc tcgtcattgg cggtactggc tcgggcaaga ccacgctcgt caacgcgatc 27360 atcaatgaaa tggtcgcctt caacccgtct gagcgcgtcg tcatcatcga ggacaccggc 27420 gaaatccagt gcgccgcaga gaacgccgtc caataccaca ccagcatcga cgtctcgatg 27480 acgctgctgc tcaagacaac gctgcgtatg cgccccgacc gcatcctggt cggtgaggta 27540 cgtggccccg aagcccttga tctgttgatg gcctggaaca ccgggcatga aggaggtgcc 27600 gccaccctgc acgcaaacaa ccccaaagcg ggcctgagcc ggctcgccat gcttatcagc 27660 atgcacccgg attcaccgaa acccattgag ccgctgattg gcgaggcggt tcatgtggtc 27720 gtccatatcg ccaggacccc tagcggccgt cgagtgcaag aaattctcga agttcttggt 27780 tacgagaacg gccagtacat caccaaaacc ctgtaaggag tatttccaat gacaacggct 27840 gttccgttcc gtctgaccat gaatcgcggc attttgttct accttgccgt gttcttcgtt 27900 ctcgctctcg cgttatccgc gcatccggcg atggcctcgg aaggcaccgg cggcagcttg 27960 ccatatgaga gctggctgac gaacctgcgc aactccgtaa ccggcccggt ggccttcgcg 28020 ctgtccatca tcggcatcgt cgtcgccggc ggcgtgctga tcttcggcgg cgaactcaac 28080 gccttcttcc gaaccctgat cttcctggtt ctggtgatgg cgctgctggt cggcgcgcag 28140 aacgtgatga gcaccttctt cggtcgtggt gccgaaatcg cggccctcgg caacggggcg 28200 ctgcaccagg tgcaagtcgc ggcggcggat gccgtgcgtg cggtagcggc tggacggctc 28260 gcctaatcat ggctctgcgc acgatcccca tccgtcgcgc aggcaaccga gaaaacctgt 28320 tcatgggtgg tgatcgtgaa ctggtgatgt tctcgggcct gatggcgttt gcgctgattt 28380 tcagcgccca agagctgcgg gccaccgtgg tcggtctgat cctgtggttc ggggcgctct 28440 atgcgttccg aatcatggcg aaggccgatc cgaagatgcg gttcgtgtac ctgcgtcacc 28500 gccggtacaa gccgtattac ccggcccgct cgaccccgtt ccgcgagaac accaatagcc 28560 aagggaagca ataccgatga tccaagcaat tgcgattgca atcgcgggcc tcggcgcgct 28620 tctgttgttc atcctctttg cccgcatccg cgcggtcgat gccgaactga aactgaaaaa 28680 gcatcgttcc aaggacgccg gcctggccga tctgctcaac tacgccgctg tcgtcgatga 28740 cggcgtaatc gtgggcaaga acggcagctt tatggctgcc tggctgtaca agggcgatga 28800 caacgcaagc agcaccgacc agcagcgcga agtagtgtcc gcccgcatca accaggccct 28860 cgcgggcctg ggaagtgggt ggatgatcca tgtggacgcc gtgcggcgtc ctgctccgaa 28920 ctacgcggag cggggcctgt cggcgttccc tgaccgtctg acggcagcga ttgaagaaga 28980 gcgctcggtc ttgccttgct cgtcggtgat gtacttcacc agctccgcga agtcgctctt 29040 cttgatggag cgcatgggga cgtgcttggc aatcacgcgc accccccggc cgttttagcg 29100 gctaaaaaag tcatggctct gccctcgggc ggaccacgcc catcatgacc ttgccaagct 29160 cgtcctgctt ctcttcgatc ttcgccagca gggcgaggat cgtggcatca ccgaaccgcg 29220 ccgtgcgcgg gtcgtcggtg agccagagtt tcagcaggcc gcccaggcgg cccaggtcgc 29280 cattgatgcg ggccagctcg cggacgtgct catagtccac gacgcccgtg attttgtagc 29340 cctggccgac ggccagcagg taggccgaca ggctcatgcc ggccgccgcc gccttttcct 29400 caatcgctct tcgttcgtct ggaaggcagt acaccttgat aggtgggctg cccttcctgg 29460 ttggcttggt ttcatcagcc atccgcttgc cctcatctgt tacgccggcg gtagccggcc 29520 agcctcgcag agcaggattc ccgttgagca ccgccaggtg cgaataaggg acagtgaaga 29580 aggaacaccc gctcgcgggt gggcctactt cacctatcct gcccggctga cgccgttgga 29640 tacaccaagg aaagtctaca cgaacccttt ggcaaaatcc tgtatatcgt gcgaaaaagg 29700 atggatatac cgaaaaaatc gctataatga ccccgaagca gggttatgca gcggaaaagc 29760 gctgcttccc tgctgttttg tggaatatct accgactgga aacaggcaaa tgcaggaaat 29820 tactgaactg aggggacagg cgagagacga tgccaaagag ctacaccgac gagctggccg 29880 agtgggttga atcccgcgcg gccaagaagc gccggcgtga tgaggctgcg gttgcgttcc 29940 tggcggtgag ggcggatgtc gaggcggcgt tagcgtccgg ctatgcgctc gtcaccattt 30000 gggagcacat gcgggaaacg gggaaggtca agttctccta cgagacgttc cgctcgcacg 30060 ccaggcggca catcaaggcc aagcccgccg atgtgcccgc accgcaggcc aaggctgcgg 30120 aacccgcgcc ggcacccaag acgccggagc cacggcggcc gaagcagggg ggcaaggctg 30180 aaaagccggc ccccgctgcg gccccgaccg gcttcacctt caacccaaca ccggacaaaa 30240 aggatctact gtaatggcga aaattcacat ggttttgcag ggcaagggcg gggtcggcaa 30300 gtcggccatc gccgcgatca ttgcgcagta caagatggac aaggggcaga cacccttgtg 30360 catcgacacc gacccggtga acgcgacgtt cgagggctac aaggccctga acgtccgccg 30420 gctgaacatc atggccggcg acgaaattaa ctcgcgcaac ttcgacaccc tggtcgagct 30480 gattgcgccg accaaggatg acgtggtgat cgacaacggt gccagctcgt tcgtgcctct 30540 gtcgcattac ctcatcagca accaggtgcc ggctctgctg caagaaatgg ggcatgagct 30600 ggtcatccat accgtcgtca ccggcggcca ggctctcctg gacacggtga gcggcttcgc 30660 ccagctcgcc agccagttcc cggccgaagc gcttttcgtg gtctggctga acccgtattg 30720 ggggcctatc gagcatgagg gcaagagctt tgagcagatg aaggcgtaca cggccaacaa 30780 ggcccgcgtg tcgtccatca tccagattcc ggccctcaag gaagaaacct acggccgcga 30840 tttcagcgac atgctgcaag agcggctgac gttcgaccag gcgctggccg atgaatcgct 30900 cacgatcatg acgcggcaac gcctcaagat cgtgcggcgc ggcctgtttg aacagctcga 30960 cgcggcggcc gtgctatgag cgaccagatt gaagagctga tccgggagat tgcggccaag 31020 cacggcatcg ccgtcggccg cgacgacccg gtgctgatcc tgcataccat caacgcccgg 31080 ctcatggccg acagtgcggc caagcaagag gaaatccttg ccgcgttcaa ggaagagctg 31140 gaagggatcg cccatcgttg gggcgaggac gccaaggcca aagcggagcg gatgctgaac 31200 gcggccctgg cggccagcaa ggacgcaatg gcgaaggtaa tgaaggacag cgccgcgcag 31260 gcggccgaag cgatccgcag ggaaatcgac gacggccttg gccgccagct cgcggccaag 31320 gtcgcggacg cgcggcgcgt ggcgatgatg aacatgatcg ccggcggcat ggtgttgttc 31380 gcggccgccc tggtggtgtg ggcctcgtta tgaatcgcag aggcgcagat gaaaaagccc 31440 ggcgttgccg ggctttgttt ttgcgttagc tgggcttgtt tgacaggccc aagctctgac 31500 tgcgcccgcg ctcgcgctcc tgggcctgtt tcttctcctg ctcctgcttg cgcatcaggg 31560 cctggtgccg tcgggctgct tcacgcatcg aatcccagtc gccggccagc tcgggatgct 31620 ccgcgcgcat cttgcgcgtc gccagttcct cgatcttggg cgcgtgaatg cccatgcctt 31680 ccttgatttc gcgcaccatg tccagccgcg tgtgcagggt ctgcaagcgg gcttgctgtt 31740 gggcctgctg ctgctgccag gcggcctttg tacgcggcag ggacagcaag ccgggggcat 31800 tggactgtag ctgctgcaaa cgcgcctgct gacggtctac gagctgttct aggcggtcct 31860 cgatgcgctc cacctggtca tgctttgcct gcacgtagag cgcaagggtc tgctggtagg 31920 tctgctcgat gggcgcggat tctaagaggg cctgctgttc cgtctcggcc tcctgggccg 31980 cctgtagcaa atcctcgccg ctgttgccgc tggactgctt tactgccggg gactgctgtt 32040 gccctgctcg cgccgtcgtc gcagttcggc ttgcccccac tcgattgact gcttcatttc 32100 gagccgcagc gatgcgatct cggattgcgt caacggacgg ggcagcgcgg aggtgtccgg 32160 cttctccttg ggtgagtcgg tcgatgccat agccaaaggt ttccttccaa aatgcgtcca 32220 ttgctggacc gtgtttctca ttgatgcccg caagcatctt cggcttgacc gccaggtcaa 32280 gcgcgccttc atgggcggtc atgacggacg ccgccatgac cttgccgccg ttgttctcga 32340 tgtagccgcg taatgaggca atggtgccgc ccatcgtcag cgtgtcatcg acaacgatgt 32400 acttctggcc ggggatcacc tccccctcga aagtcgggtt gaacgccagg cgatgatctg 32460 aaccggctcc ggttcgggcg accttctccc gctgcacaat gtccgtttcg acctcaaggc 32520 caaggcggtc ggccagaacg accgccatca tggccggaat cttgttgttc cccgccgcct 32580 cgacggcgag gactggaacg atgcggggct tgtcgtcgcc gatcagcgtc ttgagctggg 32640 caacagtgtc gtccgaaatc aggcgctcga ccaaattaag cgccgcttcc gcgtcgccct 32700 gcttcgcagc ctggtattca ggctcgttgg tcaaagaacc aaggtcgccg ttgcgaacca 32760 ccttcgggaa gtctccccac ggtgcgcgct cggctctgct gtagctgctc aagacgcctc 32820 cctttttagc cgctaaaact ctaacgagtg cgcccgcgac tcaacttgac gctttcggca 32880 cttacctgtg ccttgccact tgcgtcatag gtgatgcttt tcgcactccc gatttcaggt 32940 actttatcga aatctgaccg ggcgtgcatt acaaagttct tccccacctg ttggtaaatg 33000 ctgccgctat ctgcgtggac gatgctgccg tcgtggcgct gcgacttatc ggccttttgg 33060 gccatataga tgttgtaaat gccaggtttc agggccccgg ctttatctac cttctggttc 33120 gtccatgcgc cttggttctc ggtctggaca attctttgcc cattcatgac caggaggcgg 33180 tgtttcattg ggtgactcct gacggttgcc tctggtgtta aacgtgtcct ggtcgcttgc 33240 cggctaaaaa aaagccgacc tcggcagttc gaggccggct ttccctagag ccgggcgcgt 33300 caaggttgtt ccatctattt tagtgaactg cgttcgattt atcagttact ttcctcccgc 33360 tttgtgtttc ctcccactcg tttccgcgtc tagccgaccc ctcaacatag cggcctcttc 33420 ttgggctgcc tttgcctctt gccgcgcttc gtcacgctcg gcttgcaccg tcgtaaagcg 33480 ctcggcctgc ctggccgcct cttgcgccgc caacttcctt tgctcctggt gggcctcggc 33540 gtcggcctgc gccttcgctt tcaccgctgc caactccgtg cgcaaactct ccgcttcgcg 33600 cctggtggcg tcgcgctcgc cgcgaagcgc ctgcatttcc tggttggccg cgtccagggt 33660 cttgcggctc tcttctttga atgcgcgggc gtcctggtga gcgtagtcca gctcggcgcg 33720 cagctcctgc gctcgacgct ccacctcgtc ggcccgctgc gtcgccagcg cggcccgctg 33780 ctcggctcct gccagggcgg tgcgtgcttc ggccagggct tgccgctggc gtgcggccag 33840 ctcggccgcc tcggcggcct gctgctctag caatgtaacg cgcgcctggg cttcttccag 33900 ctcgcgggcc tgcgcctcga aggcgtcggc cagctccccg cgcacggctt ccaactcgtt 33960 gcgctcacga tcccagccgg cttgcgctgc ctgcaacgat tcattggcaa gggcctgggc 34020 ggcttgccag agggcggcca cggcctggtt gccggcctgc tgcaccgcgt ccggcacctg 34080 gactgccagc ggggcggcct gcgccgtgcg ctggcgtcgc cattcgcgca tgccggcgct 34140 ggcgtcgttc atgttgacgc gggcggcctt acgcactgca tccacggtcg ggaagttctc 34200 ccggtcgcct tgctcgaaca gctcgtccgc agccgcaaaa atgcggtcgc gcgtctcttt 34260 gttcagttcc atgttggctc cggtaattgg taagaataat aatactctta cctaccttat 34320 cagcgcaaga gtttagctga acagttctcg acttaacggc aggtttttta gcggctgaag 34380 ggcaggcaaa aaaagccccg cacggtcggc gggggcaaag ggtcagcggg aaggggatta 34440 gcgggcgtcg ggcttcttca tgcgtcgggg ccgcgcttct tgggatggag cacgacgaag 34500 cgcgcacgcg catcgtcctc ggccctatcg gcccgcgtcg cggtcaggaa cttgtcgcgc 34560 gctaggtcct ccctggtggg caccaggggc atgaactcgg cctgctcgat gtaggtccac 34620 tccatgaccg catcgcagtc gaggccgcgt tccttcaccg tctcttgcag gtcgcggtac 34680 gcccgctcgt tgagcggctg gtaacgggcc aattggtcgt aaatggctgt cggccatgag 34740 cggcctttcc tgttgagcca gcagccgacg acgaagccgg caatgcaggc ccctggcaca 34800 accaggccga cgccgggggc aggggatggc agcagctcgc caaccaggaa ccccgccgcg 34860 atgatgccga tgccggtcaa ccagcccttg aaactatccg gccccgaaac acccctgcgc 34920 attgcctgga tgctgcgccg gatagcttgc aacatcagga gccgtttctt ttgttcgtca 34980 gtcatggtcc gccctcacca gttgttcgta tcggtgtcgg acgaactgaa atcgcaagag 35040 ctgccggtat cggtccagcc gctgtccgtg tcgctgctgc cgaagcacgg cgaggggtcc 35100 gcgaacgccg cagacggcgt atccggccgc agcgcatcgc ccagcatggc cccggtcagc 35160 gagccgccgg ccaggtagcc cagcatggtg ctgttggtcg ccccggccac cagggccgac 35220 gtgacgaaat cgccgtcatt ccctctggat tgttcgctgc tcggcggggc agtgcgccgc 35280 gccggcggcg tcgtggatgg ctcgggttgg ctggcctgcg acggccggcg aaaggtgcgc 35340 agcagctcgt tatcgaccgg ctgcggcgtc ggggccgccg ccttgcgctg cggtcggtgt 35400 tccttcttcg gctcgcgcag cttgaacagc atgatcgcgg aaaccagcag caacgccgcg 35460 cctacgcctc ccgcgatgta gaacagcatc ggattcattc ttcggtcctc cttgtagcgg 35520 aaccgttgtc tgtgcggcgc gggtggcccg cgccgctgtc tttggggatc agccctcgat 35580 gagcgcgacc agtttcacgt cggcaaggtt cgcctcgaac tcctggccgt cgtcctcgta 35640 cttcaaccag gcatagcctt ccgccggcgg ccgacggttg aggataaggc gggcagggcg 35700 ctcgtcgtgc tcgacctgga cgatggcctt tttcagcttg tccgggtccg gctccttcgc 35760 gcccttttcc ttggcgtcct taccgtcctg gtcgccgtcc tcgccgtcct ggccgtcgcc 35820 ggcctccgcg tcacgctcgg catcagtctg gccgttgaag gcatcgacgg tgttgggatc 35880 gcggcccttc tcgtccagga actcgcgcag cagcttgacc gtgccgcgcg tgatttcctg 35940 ggtgtcgtcg tcaagccacg cctcgacttc ctccgggcgc ttcttgaagg ccgtcaccag 36000 ctcgttcacc acggtcacgt cgcgcacgcg gccggtgttg aacgcatcgg cgatcttctc 36060 cggcaggtcc agcagcgtga cgtgctgggt gatgaacgcc ggcgacttgc cgatttcctt 36120 ggcgatatcg cctttcttct tgcccttcgc cagctcgcgg ccaatgaagt cggcaatttc 36180 gcgcggggtc agctcgttgc gttgcaggtt ctcgataacc tggtcggctt cgttgtagtc 36240 gttgtcgatg aacgccggga tggacttctt gccggcccac ttcgagccac ggtagcggcg 36300 ggcgccgtga ttgatgatat agcggcccgg ctgctcctgg ttctcgcgca ccgaaatggg 36360 tgacttcacc ccgcgctctt tgatcgtggc accgatttcc gcgatgctct ccggggaaaa 36420 gccggggttg tcggccgtcc gcggctgatg cggatcttcg tcgatcaggt ccaggtccag 36480 ctcgataggg ccggaaccgc cctgagacgc cgcaggagcg tccaggaggc tcgacaggtc 36540 gccgatgcta tccaacccca ggccggacgg ctgcgccgcg cctgcggctt cctgagcggc 36600 cgcagcggtg tttttcttgg tggtcttggc ttgagccgca gtcattggga aatctccatc 36660 ttcgtgaaca cgtaatcagc cagggcgcga acctctttcg atgccttgcg cgcggccgtt 36720 ttcttgatct tccagaccgg cacaccggat gcgagggcat cggcgatgct gctgcgcagg 36780 ccaacggtgg ccggaatcat catcttgggg tacgcggcca gcagctcggc ttggtggcgc 36840 gcgtggcgcg gattccgcgc atcgaccttg ctgggcacca tgccaaggaa ttgcagcttg 36900 gcgttcttct ggcgcacgtt cgcaatggtc gtgaccatct tcttgatgcc ctggatgctg 36960 tacgcctcaa gctcgatggg ggacagcaca tagtcggccg cgaagagggc ggccgccagg 37020 ccgacgccaa gggtcggggc cgtgtcgatc aggcacacgt cgaagccttg gttcgccagg 37080 gccttgatgt tcgccccgaa cagctcgcgg gcgtcgtcca gcgacagccg ttcggcgttc 37140 gccagtaccg ggttggactc gatgagggcg aggcgcgcgg cctggccgtc gccggctgcg 37200 ggtgcggttt cggtccagcc gccggcaggg acagcgccga acagcttgct tgcatgcagg 37260 ccggtagcaa agtccttgag cgtgtaggac gcattgccct gggggtccag gtcgatcacg 37320 gcaacccgca agccgcgctc gaaaaagtcg aaggcaagat gcacaagggt cgaagtcttg 37380 ccgacgccgc ctttctggtt ggccgtgacc aaagttttca tcgtttggtt tcctgttttt 37440 tcttggcgtc cgcttcccac ttccggacga tgtacgcctg atgttccggc agaaccgccg 37500 ttacccgcgc gtacccctcg ggcaagttct tgtcctcgaa cgcggcccac acgcgatgca 37560 ccgcttgcga cactgcgccc ctggtcagtc ccagcgacgt tgcgaacgtc gcctgtggct 37620 tcccatcgac taagacgccc cgcgctatct cgatggtctg ctgccccact tccagcccct 37680 ggatcgcctc ctggaactgg ctttcggtaa gccgtttctt catggataac acccataatt 37740 tgctccgcgc cttggttgaa catagcggtg acagccgcca gcacatgaga gaagtttagc 37800 taaacatttc tcgcacgtca acacctttag ccgctaaaac tcgtccttgg cgtaacaaaa 37860 caaaagcccg gaaaccgggc tttcgtctct tgccgcttat ggctctgcac ccggctccat 37920 caccaacagg tcgcgcacgc gcttcactcg gttgcggatc gacactgcca gcccaacaaa 37980 gccggttgcc gccgccgcca ggatcgcgcc gatgatgccg gccacaccgg ccatcgccca 38040 ccaggtcgcc gccttccggt tccattcctg ctggtactgc ttcgcaatgc tggacctcgg 38100 ctcaccatag gctgaccgct cgatggcgta tgccgcttct ccccttggcg taaaacccag 38160 cgccgcaggc ggcattgcca tgctgcccgc cgctttcccg accacgacgc gcgcaccagg 38220 cttgcggtcc agaccttcgg ccacggcgag ctgcgcaagg acataatcag ccgccgactt 38280 ggctccacgc gcctcgatca gctcttgcac tcgcgcgaaa tccttggcct ccacggccgc 38340 catgaatcgc gcacgcggcg aaggctccgc agggccggcg tcgtgatcgc cgccgagaat 38400 gcccttcacc aagttcgacg acacgaaaat catgctgacg gctatcacca tcatgcagac 38460 ggatcgcacg aacccgctga attgaacacg agcacggcac ccgcgaccac tatgccaaga 38520 atgcccaagg taaaaattgc cggccccgcc atgaagtccg tgaatgcccc gacggccgaa 38580 gtgaagggca ggccgccacc caggccgccg ccctcactgc ccggcacctg gtcgctgaat 38640 gtcgatgcca gcacctgcgg cacgtcaatg cttccgggcg tcgcgctcgg gctgatcgcc 38700 catcccgtta ctgccccgat cccggcaatg gcaaggactg ccagcgctgc catttttggg 38760 gtgaggccgt tcgcggccga ggggcgcagc ccctgggggg atgggaggcc cgcgttagcg 38820 ggccgggagg gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag 38880 ggcgcagccc tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac 38940 aggttagcgg tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt 39000 ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag 39060 tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag 39120 ggcacttatc cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt 39180 ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc 39240 atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct 39300 gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc 39360 acacggcttc gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc 39420 ggcgagggca accagcccgg tgagcgtcgg aaaggcgctg gaagccccgt agcgacgcgg 39480 agaggggcga gacaagccaa gggcgcaggc tcgatgcgca gcacgacata gccggttctc 39540 gcaaggacga gaatttccct gcggtgcccc tcaagtgtca atgaaagttt ccaacgcgag 39600 ccattcgcga gagccttgag tccacgctag atgagagctt tgttgtaggt ggaccagttg 39660 gtgattttga acttttgctt tgccacggaa cggtctgcgt tgtcgggaag atgcgtgatc 39720 tgatccttca actcagcaaa agttcgattt attcaacaaa gccacgttgt gtctcaaaat 39780 ctctgatgtt acattgcaca agataaaaat atatcatcat gaacaataaa actgtctgct 39840 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg 39900 ac 39902 // ID AB027255; SV 2; linear; other DNA; STD; SYN; 36909 BP. XX AC AB027255; XX DT 01-JUN-1999 (Rel. 59, Created) DT 15-FEB-2005 (Rel. 82, Last updated, Version 6) XX DE Cloning vector pSB1 tetR, tetA genes with Agrobacterium tumefaciens DNA DE insert. XX KW . XX OS Cloning vector pSB1 OC other sequences; artificial sequences; vectors. XX OS Agrobacterium tumefaciens OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; OC Rhizobium/Agrobacterium group; Agrobacterium; OC Agrobacterium tumefaciens complex. XX RN [1] RP 1-36909 RA Komari T., Kuraya Y., Murai N., Ueki J., Saito Y.; RT ; RL Submitted (10-MAY-1999) to the INSDC. RL Jun Ueki, Japan Tobacco Inc., Plant Innovation Center; 700 Higashibara, RL Iwata, Shizuoka, 438-0802, Japan (E-mail:jun.ueki@ims.jti.co.jp, RL Tel:81-538-32-7120, Fax:81-538-33-6046) XX RN [2] RX DOI; 10.1046/j.1365-313X.1996.10010165.x. RX PUBMED; 8758986. RA Komari T., Hiei Y., Saito Y., Murai N., Kumashiro T.; RT "Vectors carrying two separate T-DNAs for co-transformation of higher RT plants mediated by Agrobacterium tumefaciens and segregation of RT transformants free from selection markers"; RL Plant J. 10(1):165-174(1996). XX DR MD5; 10055bbe69f4fc0d940ae5a3f0849fd6. DR EuropePMC; PMC5945794; 29687284. XX FH Key Location/Qualifiers FH FT source 1..36909 FT /organism="Cloning vector pSB1" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:94003" FT source 1065..12851 FT /organism="Agrobacterium tumefaciens" FT /mol_type="other DNA" FT /db_xref="taxon:358" FT misc_feature 1065..2370 FT /note="The virC1 gene in the virC operon in this vector was FT spontaneously mutated during the cloning process. The FT original sequence in pTiBo542 (acc#AB027257) has a C FT between nucleotides 1215 and 1226 in this sequence. Thus, FT the virC1 in this vector is probably inactive." FT /note="virC operon" FT misc_feature complement(2481..3284) FT /note="virG operon" FT misc_feature complement(3416..12851) FT /note="virB" FT rep_origin complement(15711..15890) FT /note="ColE1 origin of replication" FT misc_feature 16630 FT /note="cos site" FT CDS complement(18332..18982) FT /codon_start=1 FT /transl_table=11 FT /gene="tetR" FT /protein_id="BAA78028.1" FT /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWHF FT RNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAGTR FT PGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGGTVE FT QAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD" FT CDS 19088..20287 FT /codon_start=1 FT /transl_table=11 FT /gene="tetA" FT /protein_id="BAA78029.1" FT /translation="MKPNIPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAHY FT GILLALYALVQFACAPVLGALSDRFGRRPILLVSLAGATVDYAIMATAPFLWVLYIGRI FT VAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPHAPF FT FAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAVFFIM FT QLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGERRALM FT LGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQGQLQGSL FT AALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSGAGQRADR FT " FT misc_feature complement(20918..23107) FT /note="Rep operon" FT oriT complement(26522..26633) FT /note="orgin of transfer" FT misc_feature complement(28473..34743) FT /note="Ctl, central control operon" FT rep_origin complement(36847) FT /note="oriV, around this region" XX SQ Sequence 36909 BP; 7791 A; 10714 C; 10313 G; 8091 T; 0 other; tctagagctc gttcctcgag gcctcgaggc ctcgaggaac ggtacctgcg gggaagctta 60 caataatgtg tgttgttaag tcttgttgcc tgtcatcgtc tgactgactt tcgtcataaa 120 tcccggcctc cgtaacccag ctttgggcaa gctcacggat ttgatccggc ggaacgggaa 180 tatcgagatg ccgggctgaa cgctgcagtt ccagctttcc ctttcgggac aggtactcca 240 gctgattgat tatctgctga agggtcttgg ttccacctcc tggcacaatg cgaatgatta 300 cttgagcgcg atcgggcatc caattttctc ccgtcaggtg cgtggtcaag tgctacaagg 360 cacctttcag taacgagcga ccgtcgatcc gtcgccggga tacggacaaa atggagcgca 420 gtagtccatc gagggcggcg aaagcctcgc caaaagcaat acgttcatct cgcacagcct 480 ccagatccga tcgagggtct tcggcgtagg cagatagaag catggataca ttgcttgaga 540 gtattccgat ggactgaagt atggcttcca tcttttctcg tgtgtctgca tctatttcga 600 gaaagccccc gatgcggcgc accgcaacgc gaattgccat actatccgaa agtcccagca 660 ggcgcgcttg ataggaaaag gtttcatact cggccgatcg cagacgggca ctcacgacct 720 tgaacccttc aactttcagg gatcgatgct ggttgatggt agtctcactc gacgtggctc 780 tggtgtgttt tgacatagct tcctccaaag aaagcggaag gtctggatac tccagcacga 840 aatgtgcccg ggtagacgga tggaagtcta gccctgctca atatgaaatc aacagtacat 900 ttacagtcaa tactgaatat acttgctaca tttgcaattg tcttataacg aatgtgaaat 960 aaaaatagtg taacaacgct tttactcatc gataatcaca aaaacattta tacgaacaaa 1020 aatacaaatg cactccggtt tcacaggata ggcgggatca gaatatgcaa cttttgacgt 1080 tttgttcttt caaagggggt gctggcaaaa ccaccgcact catgggcctt tgcgctgctt 1140 tggcaaatga cggtaaacga gtggccctct ttgatgccga cgaaaaccgg cctctgacgc 1200 gatggagaga aaacgcctta caaagcagta ctgggatcct cgctgtgaag tctattccgc 1260 cgacgaaatg ccccttcttg aagcagccta tgaaaatgcc gagctcgaag gatttgatta 1320 tgcgttggcc gatacgcgtg gcggctcgag cgagctcaac aacacaatca tcgctagctc 1380 aaacctgctt ctgatcccca ccatgctaac gccgctcgac atcgatgagg cactatctac 1440 ctaccgctac gtcatcgagc tgctgttgag tgaaaatttg gcaattccta cagctgtttt 1500 gcgccaacgc gtcccggtcg gccgattgac aacatcgcaa cgcaggatgt cagagacgct 1560 agagagcctt ccagttgtac cgtctcccat gcatgaaaga gatgcatttg ccgcgatgaa 1620 agaacgcggc atgttgcatc ttacattact aaacacggga actgatccga cgatgcgcct 1680 catagagagg aatcttcgga ttgcgatgga ggaagtcgtg gtcatttcga aactgatcag 1740 caaaatcttg gaggcttgaa gatggcaatt cgcaagcccg cattgtcggt cggcgaagca 1800 cggcggcttg ctggtgctcg acccgagatc caccatccca acccgacact tgttccccag 1860 aagctggacc tccagcactt gcctgaaaaa gccgacgaga aagaccagca acgtgagcct 1920 ctcgtcgccg atcacattta cagtcccgat cgacaactta agctaactgt ggatgccctt 1980 agtccacctc cgtccccgaa aaagctccag gtttttcttt cagcgcgacc gcccgcgcct 2040 caagtgtcga aaacatatga caacctcgtt cggcaataca gtccctcgaa gtcgctacaa 2100 atgattttaa ggcgcgcgtt ggacgatttc gaaagcatgc tggcagatgg atcatttcgc 2160 gtggccccga aaagttatcc gatcccttca actacagaaa aatccgttct cgttcagacc 2220 tcacgcatgt tcccggttgc gttgctcgag gtcgctcgaa gtcattttga tccgttgggg 2280 ttggagaccg ctcgagcttt cggccacaag ctggctaccg ccgcgctcgc gtcattcttt 2340 gctggagaga agccatcgag caattggtga agagggacct atcggaaccc ctcaccaaat 2400 attgagtgta ggtttgaggc cgctggccgc gtcctcagtc accttttgag ccagataatt 2460 aagagccaaa tgcaattggc tcaggctgcc atcgtccccc cgtgcgaaac ctgcacgtcc 2520 gcgtcaaaga aataaccggc acctcttgct gtttttatca gttgagggct tgacggatcc 2580 gcctcaagtt tgcggcgcag ccgcaaaatg agaacatcta tactcctgtc gtaaacctcc 2640 tcgtcgcgta ctcgactggc aatgagaagt tgctcgcgcg atagaacgtc gcggggtttc 2700 tctaaaaacg cgaggagaag attgaactca cctgccgtaa gtttcacctc accgccagct 2760 tcggacatca agcgacgttg cctgagatta agtgtccagt cagtaaaaca aaaagaccgt 2820 cggtctttgg agcggacaac gttggggcgc acgcgcaagg caacccgaat gcgtgcaaga 2880 aactctctcg tactaaacgg cttagcgata aaatcacttg ctcctagctc gagtgcaaca 2940 actttatccg tctcctcaag gcggtcgcca ctgataatta tgattggaat atcagacttt 3000 gccgccagat ttcgaacgat ctcaagccca tcttcacgac ctaaatttag atcaacaacc 3060 acgacatcga ccgtcgcgga agagagtact ctagtgaact gggtgctgtc ggctaccgcg 3120 gtcactttga aggcgtggat cgtaaggtat tcgataataa gatgccgcat agcgacatcg 3180 tcatcgataa gaagaacgtg tttcaacggc tcacctttca atctaaaatc tgaacccttg 3240 ttcacagcgc ttgagaaatt ttcacgtgaa ggatgtacaa tcatctccag ctaaatgggc 3300 agttcgtcag aattgcggct gaccgcggat gacgaaaatg cgaaccaagt atttcaattt 3360 tatgacaaaa gttctcaatc gttgttacaa gtgaaacgct tcgaggttac agctactatt 3420 gattaaggag atcgcctatg gtctcgcccc ggcgtcgtgc gtccgccgcg agccagatct 3480 cgcctacttc ataaacgtcc tcataggcac ggaatggaat gatgacatcg atcgccgtag 3540 agagcatgtc aatcagtgtg cgatcttcca agctagcacc ttgggcgcta cttttgacaa 3600 gggaaaacag tttcttgaat ccttggattg gattcgcgcc gtgtattgtt gaaatcgatc 3660 ccggatgtcc cgagacgact tcactcagat aagcccatgc tgcatcgtcg cgcatctcgc 3720 caagcaatat ccggtccggc cgcatacgca gacttgcttg gagcaagtgc tcggcgctca 3780 cagcacccag cccagcaccg ttcttggagt agagtagtct aacatgatta tcgtgtggaa 3840 tgacgagttc gagcgtatct tctatggtga ttagcctttc ctgggggggg atggcgctga 3900 tcaaggtctt gctcattgtt gtcttgccgc ttccggtagg gccacatagc aacatcgtca 3960 gtcggctgac gacgcatgcg tgcagaaacg cttccaaatc cccgttgtca aaatgctgaa 4020 ggatagcttc atcatcctga ttttggcgtt tccttcgtgt ctgccactgg ttccacctcg 4080 aagcatcata acgggaggag acttctttaa gaccagaaac acgcgagctt ggccgtcgaa 4140 tggtcaagct gacggtgccc gagggaacgg tcggcggcag acagatttgt agtcgttcac 4200 caccaggaag ttcagtggcg cagagggggt tacgtggtcc gacatcctgc tttctcagcg 4260 cgcccgctaa aatagcgata tcttcaagat catcataaga gacgggcaaa ggcatcttgg 4320 taaaaatgcc ggcttggcgc acaaatgcct ctccaggtcg attgatcgca atttcttcag 4380 tcttcgggtc atcgagccat tccaaaatcg gcttcagaag aaagcgtagt tgcggatcca 4440 cttccattta caatgtatcc tatctctaag cggaaatttg aattcattaa gagcggcggt 4500 tcctcccccg cgtggcgccg ccagtcaggc ggagctggta aacaccaaag aaatcgaggt 4560 cccgtgctac gaaaatggaa acggtgtcac cctgattctt cttcagggtt ggcggtatgt 4620 tgatggttgc cttaagggct gtctcagttg tctgctcacc gttattttga aagctgttga 4680 agctcatccc gccacccgag ctgccggcgt aggtgctagc tgcctggaag gcgccttgaa 4740 caacactcaa gagcatagct ccgctaaaac gctgccagaa gtggctgtcg accgagcccg 4800 gcaatcctga gcgaccgagt tcgtccgcgc ttggcgatgt taacgagatc atcgcatggt 4860 caggtgtctc ggcgcgatcc cacaacacaa aaacgcgccc atctccctgt tgcaagccac 4920 gctgtatttc gccaacaacg gtggtgccac gatcaagaag cacgatattg ttcgttgttc 4980 cacgaatatc ctgaggcaag acacacttta catagcctgc caaatttgtg tcgattgcgg 5040 tttgcaagat gcacggaatt attgtccctt gcgttaccat aaaatcgggg tgcggcaaga 5100 gcgtggcgct gctgggctgc agctcggtgg gtttcatacg tatcgacaaa tcgttctcgc 5160 cggacacttc gccattcggc aaggagttgt cgtcacgctt gccttcttgt cttcggcccg 5220 tgtcgccctg aatggcgcgt ttgctgaccc cttgatcgcc gctgctatat gcaaaaatcg 5280 gtgtttcttc cggccgtggc tcatgccgct ccggttcgcc cctcggcggt agaggagcag 5340 caggctgaac agcctcttga accgctggag gatccggcgg cacctcaatc ggagctggat 5400 gaaatggctt ggtgtttgtt gcgatcaaag ttgacggcga tgcgttctca ttcaccttct 5460 tttggcgccc acctagccaa atgaggctta atgataacgc gagaacgaca cctccgacga 5520 tcaatttctg agaccccgaa agacgccggc gatgtttgtc ggagaccagg gatccagatg 5580 catcaacctc atgtgccgct tgctgactat cgttattcat cccttcgccc ccttcaggac 5640 gcgtttcaca tcgggcctca ccgtgcccgt ttgcggcctt tggccaacgg gatcgtaagc 5700 ggtgttccag atacatagta ctgtgtggcc atccctcaga cgccaacctc gggaaaccga 5760 agaaatctcg acatcgctcc ctttaactga atagttggca acagcttcct tgccatcagg 5820 attgatggtg tagatggagg gtatgcgtac attgcccgga aagtggaata ccgtcgtaaa 5880 tccattgtcg aagacttcga gtggcaacag cgaacgatcg ccttgggcga cgtagtgcca 5940 attactgtcc gccgcaccaa gggctgtgac aggctgatcc aataaattct cagctttccg 6000 ttgatattgt gcttccgcgt gtagtctgtc cacaacagcc ttctgttgtg cctcccttcg 6060 ccgagccgcc gcatcgtcgg cggggtaggc gaattggacg ctgtaataga gatcgggctg 6120 ctctttatcg aggtgggaca gagtcttgga acttatactg aaaacataac ggcgcatccc 6180 ggagtcgctt gcggttagca cgattactgg ctgaggcgtg aggacctggc ttgccttgaa 6240 aaatagataa tttccccgcg gtagggctgc tagatctttg ctatttgaaa cggcaaccgc 6300 tgtcaccgtt tcgttcgtgg cgaatgttac gaccaaagta gctccaaccg ccgtcgagag 6360 gcgcaccact tgatcgggat tgtaagccaa ataacgcatg cgcggatcta gcttgcccgc 6420 cattggagtg tcttcagcct ccgcaccagt cgcagcggca aataaacatg ctaaaatgaa 6480 aagtgctttt ctgatcatgg ttcgctgtgg cctacgtttg aaacggtatc ttccgatgtc 6540 tgataggagg tgacaaccag acctgccggg ttggttagtc tcaatctgcc gggcaagctg 6600 gtcacctttt cgtagcgaac tgtcgcggtc cacgtactca ccacaggcat tttgccgtca 6660 acgacgaggg tccttttata gcgaatttgc tgcgtgcttg gagttacatc atttgaagcg 6720 atgtgctcga cctccaccct gccgcgtttg ccaagaatga cttgaggcga actgggattg 6780 ggatagttga agaattgctg gtaatcctgg cgcactgttg gggcactgaa gttcgatacc 6840 aggtcgtagg cgtactgagc ggtgtcggca tcataactct cgcgcaggcg aacgtactcc 6900 cacaatgagg cgttaacgac ggcctcctct tgagttgcag gcaatcgcga gacagacacc 6960 tcgctgtcaa cggtgccgtc cggccgtatc catagatata cgggcacaag cctgctcaac 7020 ggcaccattg tggctatagc gaacgcttga gcaacatttc ccaaaatcgc gatagctgcg 7080 acagctgcaa tgagtttgga gagacgtcgc gccgatttcg ctcgcgcggt ttgaaaggct 7140 tctacttcct tatagtgctc ggcaaggctt tcgcgcgcca ctagcatggc atattcaggc 7200 cccgtcatag cgtccacccg aattgccgag ctgaagatct gacggagtag gctgccatcg 7260 ccccacattc agcgggaaga tcgggccttt gcagctcgct aatgtgtcgt ttgtctggca 7320 gccgctcaaa gcgacaacta ggcacagcag gcaatacttc atagaattct ccattgaggc 7380 gaatttttgc gcgacctagc ctcgctcaac ctgagcgaag cgacggtaca agctgctggc 7440 agattgggtt gcgccgctcc agtaactgcc tccaatgttg ccggcgatcg ccggcaaagc 7500 gacaatgagc gcatcccctg tcagaaaaaa catatcgagt tcgtaaagac caatgatctt 7560 ggccgcggtc gtaccggcga aggtgattac accaagcata agggtgagcg cagtcgcttc 7620 ggttaggatg acgatcgttg ccacgaggtt taagaggaga agcaagagac cgtaggtgat 7680 aagttgcccg atccacttag ctgcgatgtc ccgcgtgcga tcaaaaatat atccgacgag 7740 gatcagaggc ccgatcgcga gaagcacttt cgtgagaatt ccaacggcgt cgtaaactcc 7800 gaaggcagac cagagcgtgc cgtaaaggac ccactgtgcc ccttggaaag caaggatgtc 7860 ctggtcgttc atcggaccga tttcggatgc gattttctga aaaacggcct gggtcacggc 7920 gaacattgta tccaactgtg ccggaacagt ctgcagaggc aagccggtta cactaaactg 7980 ctgaacaaag tttgggaccg tcttttcgaa gatggaaacc acatagtctt ggtagttagc 8040 ctgcccaaca attagagcaa caacgatggt gaccgtgatc acccgagtga taccgctacg 8100 ggtatcgact tcgccgcgta tgactaaaat accctgaaca ataatccaaa gagtgacaca 8160 ggcgatcaat ggcgcactca ccgcctcctg gatagtctca agcatcgagt ccaagcctgt 8220 cgtgaaggct acatcgaaga tcgtatgaat ggccgtaaac ggcgccggaa tcgtgaaatt 8280 catcgattgg acctgaactt gactggtttg tcgcataatg ttggataaaa tgagctcgca 8340 ttcggcgagg atgcgggcgg atgaacaaat cgcccagcct taggggaggg caccaaagat 8400 gacagcggtc ttttgatgct ccttgcgttg agcggccgcc tcttccgcct cgtgaaggcc 8460 ggcctgcgcg gtagtcatcg ttaataggct tgtcgcctgt acattttgaa tcattgcgtc 8520 atggatctgc ttgagaagca aaccattggt cacggttgcc tgcatgatat tgcgagatcg 8580 ggaaagctga gcagacgtat cagcattcgc cgtcaagcgt ttgtccatcg tttccagatt 8640 gtcagccgca atgccagcgc tgtttgcgga accggtgatc tgcgatcgca acaggtccgc 8700 ttcagcatca ctacccacga ctgcacgatc tgtatcgctg gtgatcgcac gtgccgtggt 8760 cgacattggc attcgcggcg aaaacatttc attgtctagg tccttcgtcg aaggatactg 8820 atttttctgg ttgagcgaag tcagtagtcc agtaacgccg taggccgacg tcaacatcgt 8880 aaccatcgct atagtctgag tgagattctc cgcagtcgcg agcgcagtcg cgagcgtctc 8940 agcctccgtt gccgggtcgc taacaacaaa ctgcgcccgc gcgggctgaa tatatagaaa 9000 gctgcaggtc aaaactgttg caataagttg cgtcgtcttc atcgtttcct accttatcaa 9060 tcttctgcct cgtggtgacg ggccatgaat tcgctgagcc agccagatga gttgccttct 9120 tgtgcctcgc gtagtcgagt tgcaaagcgc accgtgttgg cacgccccga aagcacggcg 9180 acatattcac gcatatcccg cagatcaaat tcgcagatga cgcttccact ttctcgttta 9240 agaagaaact tacggctgcc gaccgtcatg tcttcacgga tcgcctgaaa ttccttttcg 9300 gtacatttca gtccatcgac ataagccgat cgatctgcgg ttggtgatgg atagaaaatc 9360 ttcgtcatac attgcgcaac caagctggct cctagcggcg attccagaac atgctctggt 9420 tgctgcgttg ccagtattag catcccgttg ttttttcgaa cggtcaggag gaatttgtcg 9480 acgacagtcg aaaatttagg gtttaacaaa taggcgcgaa actcatcgca gctcatcaca 9540 aaacggcggc cgtcgatcat ggctccaatc cgatgcagga gatatgctgc agcgggagcg 9600 catacttcct cgtattcgag aagatgcgtc atgtcgaagc cggtaatcga cggatctaac 9660 tttacttcgt caacttcgcc gtcaaatgcc cagccaagcg catggccccg gcaccagcgt 9720 tggagccgcg ctcctgcgcc ttcggcgggc ccatgcaaca aaaattcacg taaccccgcg 9780 attgaacgca tttgtggatc aaacgagagc tgacgatgga taccacggac cagacggcgg 9840 ttctcttccg gagaaatccc accccgacca tcactctcga tgagagccac gatccattcg 9900 cgcagaaaat cgtgtgaggc tgctgtgttt tctaggccac gcaacggcgc caacccgctg 9960 ggtgtgcctc tgtgaagtgc caaatatgtt cctcctgtgg cgcgaaccag caattcgcca 10020 ccccggtcct tgtcaaagaa cacgaccgta cctgcacggt cgaccatgct ctgttcgagc 10080 atggctagaa caaacatcat gagcgtcgtc ttacccctcc cgataggccc gaatattgcc 10140 gtcatgccaa catcgtgctc atgcgggata tagtcgaaag gcgttccgcc attggtacga 10200 aatcgggcaa tcgcgttgcc ccagtggcct gagctggcgc cctctggaaa gttttcgaaa 10260 gagacaaacc ctgcgaaatt gcgtgaagtg attgcgccag ggcgtgtgcg ccacttaaaa 10320 ttccccggca attgggacca ataggccgct tccataccaa taccttcttg gacaaccacg 10380 gcacctgcat ccgccattcg tgtccgagcc cgcgcgcccc tgtccccaag actattgaga 10440 tcgtctgcat agacgcaaag gctcaaatga tgtgagccca taacgaattc gttgctcgca 10500 agtgcgtcct cagcctcgga taatttgccg atttgagtca cggctttatc gccggaactc 10560 agcatctggc tcgatttgag gctaagtttc gcgtgcgctt gcgggcgagt caggaacgaa 10620 aaactctgcg tgagaacaag tggaaaatcg agggatagca gcgcgttgag catgcccggc 10680 cgtgtttttg cagggtattc gcgaaacgaa tagatggatc caacgtaact gtcttttggc 10740 gttctgatct cgagtcctcg cttgccgcaa atgactctgt cggtataaat cgaagcgccg 10800 agtgagccgc tgacgaccgg aaccggtgtg aaccgaccag tcatgatcaa ccgtagcgct 10860 tcgccaattt cggtgaagag cacaccctgc ttctcgcgga tgccaagacg atgcaggcca 10920 tacgctttaa gagagccagc gacaacatgc caaagatctt ccatgttcct gatctggccc 10980 gtgagatcgt tttccctttt tccgcttagc ttggtgaacc tcctctttac cttccctaaa 11040 gccgcctgtg ggtagacaat caacgtaagg aagtgttcat tgcggaggag ttggccggag 11100 agcacgcgct gttcaaaagc ttcgttcagg ctagcggcga aaacactacg gaagtgtcgc 11160 ggcgccgatg atggcacgtc ggcatgacgt acgaggtgag catatattga cacatgatca 11220 tcagcgatat tgcgcaacag cgtgttgaac gcacgacaac gcgcattgcg catttcagtt 11280 tcctcaagct cgaatgcaac gccatcaatt ctcgcaatgg tcatgatcga tccgtcttca 11340 agaaggacga tatggtcgct gaggtggcca atataaggga gatagatctc accggatctt 11400 tcggtcgttc cactcgcgcc gagcatcaca ccattcctct ccctcgtggg ggaaccctaa 11460 ttggatttgg gctaacagta gcgccccccc aaactgcact atcaatgctt cttcccgcgg 11520 tccgcaaaaa tagcaggacg acgctcgccg cattgtagtc tcgctccacg atgagccggg 11580 ctgcaaacca taacggcacg agaacgactt cgtagagcgg gttctgaacg ataacgatga 11640 caaagccggc gaacatcatg aataaccctg ccaatgtcag tggcacccca agaaacaatg 11700 cgggccgtgt ggctgcgagg taaagggtcg attcttccaa acgatcagcc atcaactacc 11760 gccagtgagc gtttggccga ggaagctcgc cccaaacatg ataacaatgc cgccgacgac 11820 gccggcaacc agcccaagcg aagcccgccc gaacatccag gagatcccga tagcgacaat 11880 gccgagaaca gcgagtgact ggccgaacgg accaaggata aacgtgcata tattgttaac 11940 cattgtggcg gggtcagtgc cgccacccgc agattgcgct gcggcgggtc cggatgagga 12000 aatgctccat gcaattgcac cgcacaagct tggggcgcag ctcgatatca cgcgcatcat 12060 cgcattcgag agcgagaggc gatttagatg taaacggtat ctctcaaagc atcgcatcaa 12120 tgcgcacctc cttagtataa gtcgaataag acttgattgt cgtctgcgga tttgccgttg 12180 tcctggtgtg gcggtggcgg agcgattaaa ccgccagcgc catcctcctg cgagcggcgc 12240 tgatatgacc cccaaacatc ccacgtctct tcggatttta gcgcctcgtg atcgtctttt 12300 ggaggctcga ttaacgcggg caccagcgat tgagcagctg tttcaacttt tcgcacgtag 12360 ccgtttgcaa aaccgccgat gaaattaccg gtgttgtaag cggagatcgc ccgacgaagc 12420 gcaaattgct tctcgtcaat cgtttcgccg cctgcataac gacttttcag catgtttgca 12480 gcggcagata atgatgtgca cgcctggagc gcaccgtcag gtgtcagacc gagcatagaa 12540 aaatttcgag agtttatttg catgaggcca acatccagcg aatgccgtgc atcgagacgg 12600 tgcctgacga cttgggttgc ttggctgtga tcttgccagt gaagcgtttc gccggtcgtg 12660 ttgtcatgaa tcgctaaagg atcaaagcga ctctccacct tagctatcgc cgcaagcgta 12720 gatgtcgcaa ctgatggggc acacttgcga gcaacatggt caaactcagc agatgagagt 12780 ggcgtggcaa ggctcgacga acagaaggag accatcaagg caagagaaag cgaccccgat 12840 ctcttaagca taccttatct ccttagctcg caactaacac cgcctctccc gttggaagaa 12900 gtgcgttgtt ttatgttgaa gattatcggg agggtcggtt actcgaaaat tttcaattgc 12960 ttctttatga tttcaattga agcgagaaac ctcgcccggc gtcttggaac gcaacatgga 13020 ccgagaaccg cgcatccatg actaagcaac cggatcgacc tattcaggcc gcagttggtc 13080 aggtcaggct cagaacgaaa atgctcggcg aggttacgct gtctgtaaac ccattcgatg 13140 aacgggaagc ttccttccga ttgctcttgg caggaatatt ggcccatgcc tgcttgcgct 13200 ttgcaaatgc tcttatcgcg ttggtatcat atgccttgtc cgccagcaga aacgcactct 13260 aagcgattat ttgtaaaaat gtttcggtca tgcggcggtc atgggcttga cccgctgtca 13320 gcgcaagacg gatcggtcaa ccgtcggcat cgacaacagc gtgaatcttg gtggtcaaac 13380 cgccacggga acgtcccata cagccatcgt cttgatcccg ctgtttcccg tcgccgcatg 13440 ttggtggacg cggacacagg aactgtcaat catgacgaca ttctatcgaa agccttggaa 13500 atcacactca gaatatgatc ccagacgtct gcctcacgcc atcgtacaaa gcgattgtag 13560 caggttgtac aggaaccgta tcgatcagga acgtctgccc agggcgggcc cgtccggaag 13620 cgccacaaga tgacattgat cacccgcgtc aacgcgcggc acgcgacgcg gcttatttgg 13680 gaacaaagga ctgaacaaca gtccattcga aatcggtgac atcaaagcgg ggacgggtta 13740 tcagtggcct ccaagtcaag cctcaatgaa tcaaaatcag accgatttgc aaacctgatt 13800 tatgagtgtg cggcctaaat gatgaaatcg tccttctaga tcgcctccgt ggtgtagcaa 13860 cacctcgcag tatcgccgtg ctgaccttgg ccagggaatt gactggcaag ggtgctttca 13920 catgaccgct cttttggccg cgatagatga tttcgttgct gctttgggca cgtagaagga 13980 gagaagtcat atcggagaaa ttcctcctgg cgcgagagcc tgctctatcg cgacggcatc 14040 ccactgtcgg gaacagaccg gatcattcac gaggcgaaag tcgtcaacac atgcgttata 14100 ggcatcttcc cttgaaggat gatcttgttg ctgccaatct ggaggtgcgg cagccgcagg 14160 cagatgcgat ctcagcgcaa cttgcggcaa aacatctcac tcacctgaaa accactagcg 14220 agtctcgcga tcagacgaag gccttttact taacgacaca atatccgatg tctgcatcac 14280 aggcgtcgct atcccagtca atactaaagc ggtgcaggaa ctaaagatta ctgatgactt 14340 aggcgtgcca cgaggcctga gacgacgcgc gtagacagtt ttttgaaatc attatcaaag 14400 tgatggcctc cgctgaagcc tatcacctct gcgccggtct gtcggagaga tgggcaagca 14460 ttattacggt cttcgcgccc gtacatgcat tggacgattg cagggtcaat ggatctgaga 14520 tcatccagag gattgccgcc cttaccttcc gtttcgagtt ggagccagcc cctaaatgag 14580 acgacatagt cgacttgatg tgacaatgcc aagagagaga tttgcttaac ccgatttttt 14640 tgctcaagcg taagcctatt gaagcttgcc ggcatgacgt ccgcgccgaa agaatatcct 14700 acaagtaaaa cattctgcac accgaaatgc ttggtgtaga catcgattat gtgaccaaga 14760 tccttagcag tttcgcttgg ggaccgctcc gaccagaaat accgaagtga actgacgcca 14820 atgacaggaa tcccttccgt ctgcagatag gtaccatcga tagatctgct gcctcgcgcg 14880 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 14940 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 15000 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac 15060 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac 15120 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 15180 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 15240 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 15300 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 15360 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 15420 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 15480 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 15540 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 15600 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 15660 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 15720 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 15780 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 15840 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 15900 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 15960 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc 16020 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa 16080 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta 16140 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc 16200 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat 16260 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta 16320 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt 16380 aatagtttgc gcaacgttgt tgccattgct gcaggggggg gggggggggg gttccattgt 16440 tcattccacg gacaaaaaca gagaaaggaa acgacagagg ccaaaaagct cgctttcagc 16500 acctgtcgtt tcctttcttt tcagagggta ttttaaataa aaacattaag ttatgacgaa 16560 gaagaacgga aacgccttaa accggaaaat tttcataaat agcgaaaacc cgcgaggtcg 16620 ccgccccgta acctgtcgga tcaccggaaa ggacccgtaa agtgataatg attatcatct 16680 acatatcaca acgtgcgtgg aggccatcaa accacgtcaa ataatcaatt atgacgcagg 16740 tatcgtatta attgatctgc atcaacttaa cgtaaaaaca acttcagaca atacaaatca 16800 gcgacactga atacggggca acctcatgtc cccccccccc ccccccctgc aggcatcgtg 16860 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 16920 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 16980 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 17040 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 17100 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 17160 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 17220 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 17280 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 17340 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 17400 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 17460 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 17520 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 17580 aggccctttc gtcttcaaga attcggagct tttgccattc tcaccggatt cagtcgtcac 17640 tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa taggttgtat 17700 tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc tatggaactg 17760 cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg gtattgataa 17820 tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct aatcagaatt 17880 ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg gcggctttgt 17940 tgaataaatc gaacttttgc tgagttgaag gatcagatca cgcatcttcc cgacaacgca 18000 gaccgttccg tggcaaagca aaagttcaaa atcaccaact ggtccaccta caacaaagct 18060 ctcatcaacc gtggctccct cactttctgg ctggatgatg gggcgattca ggcctggtat 18120 gagtcagcaa caccttcttc acgaggcaga cctcagcgcc agaaggccgc cagagaggcc 18180 gagcgcggcc gtgaggcttg gacgctaggg cagggcatga aaaagcccgt agcgggctgc 18240 tacgggcgtc tgacgcggtg gaaaggggga ggggatgttg tctacatggc tctgctgtag 18300 tgagtgggtt gcgctccggc agcggtcctg atcaatcgtc accctttctc ggtccttcaa 18360 cgttcctgac aacgagcctc cttttcgcca atccatcgac aatcaccgcg agtccctgct 18420 cgaacgctgc gtccggaccg gcttcgtcga aggcgtctat cgcggcccgc aacagcggcg 18480 agagcggagc ctgttcaacg gtgccgccgc gctcgccggc atcgctgtcg ccggcctgct 18540 cctcaagcac ggccccaaca gtgaagtagc tgattgtcat cagcgcattg acggcgtccc 18600 cggccgaaaa acccgcctcg cagaggaagc gaagctgcgc gtcggccgtt tccatctgcg 18660 gtgcgcccgg tcgcgtgccg gcatggatgc gcgcgccatc gcggtaggcg agcagcgcct 18720 gcctgaagct gcgggcattc ccgatcagaa atgagcgcca gtcgtcgtcg gctctcggca 18780 ccgaatgcgt atgattctcc gccagcatgg cttcggccag tgcgtcgagc agcgcccgct 18840 tgttcctgaa gtgccagtaa agcgccggct gctgaacccc caaccgttcc gccagtttgc 18900 gtgtcgtcag accgtctacg ccgacctcgt tcaacaggtc cagggcggca cggatcactg 18960 tattcggctg caactttgtc atgcttgaca ctttatcact gataaacata atatgtccac 19020 caacttatca gtgataaaga atccgcgcgt tcaatcggac cagcggaggc tggtccggag 19080 gccagacgtg aaacccaaca tacccctgat cgtaattctg agcactgtcg cgctcgacgc 19140 tgtcggcatc ggcctgatta tgccggtgct gccgggcctc ctgcgcgatc tggttcactc 19200 gaacgacgtc accgcccact atggcattct gctggcgctg tatgcgttgg tgcaatttgc 19260 ctgcgcacct gtgctgggcg cgctgtcgga tcgtttcggg cggcggccaa tcttgctcgt 19320 ctcgctggcc ggcgccactg tcgactacgc catcatggcg acagcgcctt tcctttgggt 19380 tctctatatc gggcggatcg tggccggcat caccggggcg actggggcgg tagccggcgc 19440 ttatattgcc gatatcactg atggcgatga gcgcgcgcgg cacttcggct tcatgagcgc 19500 ctgtttcggg ttcgggatgg tcgcgggacc tgtgctcggt gggctgatgg gcggtttctc 19560 cccccacgct ccgttcttcg ccgcggcagc cttgaacggc ctcaatttcc tgacgggctg 19620 tttccttttg ccggagtcgc acaaaggcga acgccggccg ttacgccggg aggctctcaa 19680 cccgctcgct tcgttccggt gggcccgggg catgaccgtc gtcgccgccc tgatggcggt 19740 cttcttcatc atgcaacttg tcggacaggt gccggccgcg ctttgggtca ttttcggcga 19800 ggatcgcttt cactgggacg cgaccacgat cggcatttcg cttgccgcat ttggcattct 19860 gcattcactc gcccaggcaa tgatcaccgg ccctgtagcc gcccggctcg gcgaaaggcg 19920 ggcactcatg ctcggaatga ttgccgacgg cacaggctac atcctgcttg ccttcgcgac 19980 acggggatgg atggcgttcc cgatcatggt cctgcttgct tcgggtggca tcggaatgcc 20040 ggcgctgcaa gcaatgttgt ccaggcaggt ggatgaggaa cgtcaggggc agctgcaagg 20100 ctcactggcg gcgctcacca gcctgacctc gatcgtcgga cccctcctct tcacggcgat 20160 ctatgcggct tctataacaa cgtggaacgg gtgggcatgg attgcaggcg ctgccctcta 20220 cttgctctgc ctgccggcgc tgcgtcgcgg gctttggagc ggcgcagggc aacgagccga 20280 tcgctgatcg tggaaacgat aggcctatgc catgcgggtc aaggcgactt ccggcaagct 20340 atacgcgccc taggagtgcg gttggaacgt tggcccagcc agatactccc gatcacgagc 20400 aggacgccga tgatttgaag cgcactcagc gtctgatcca agaacaacca tcctagcaac 20460 acggcggtcc ccgggctgag aaagcccagt aaggaaacaa ctgtaggttc gagtcgcgag 20520 atcccccgga accaaaggaa gtaggttaaa cccgctccga tcaggccgag ccacgccagg 20580 ccgagaacat tggttcctgt aggcatcggg attggcggat caaacactaa agctactgga 20640 acgagcagaa gtcctccggc cgccagttgc caggcggtaa aggtgagcag aggcacggga 20700 ggttgccact tgcgggtcag cacggttccg aacgccatgg aaaccgcccc cgccaggccc 20760 gctgcgacgc cgacaggatc tagcgctgcg tttggtgtca acaccaacag cgccacgccc 20820 gcagttccgc aaatagcccc caggaccgcc atcaatcgta tcgggctacc tagcagagcg 20880 gcagagatga acacgaccat cagcggctgc acagcgccta ccgtcgccgc gaccccgccc 20940 ggcaggcggt agaccgaaat aaacaacaag ctccagaata gcgaaatatt aagtgcgccg 21000 aggatgaaga tgcgcatcca ccagattccc gttggaatct gtcggacgat catcacgagc 21060 aataaacccg ccggcaacgc ccgcagcagc ataccggcga cccctcggcc tcgctgttcg 21120 ggctccacga aaacgccgga cagatgcgcc ttgtgagcgt ccttggggcc gtcctcctgt 21180 ttgaagaccg acagcccaat gatctcgccg tcgatgtagg cgccgaatgc cacggcatct 21240 cgcaaccgtt cagcgaacgc ctccatgggc tttttctcct cgtgctcgta aacggacccg 21300 aacatctctg gagctttctt cagggccgac aatcggatct cgcggaaatc ctgcacgtcg 21360 gccgctccaa gccgtcgaat ctgagcctta atcacaattg tcaattttaa tcctctgttt 21420 atcggcagtt cgtagagcgc gccgtgcgtc ccgagcgata ctgagcgaag caagtgcgtc 21480 gagcagtgcc cgcttgttcc tgaaatgcca gtaaagcgct ggctgctgaa cccccagccg 21540 gaactgaccc cacaaggccc tagcgtttgc aatgcaccag gtcatcattg acccaggcgt 21600 gttccaccag gccgctgcct cgcaactctt cgcaggcttc gccgacctgc tcgcgccact 21660 tcttcacgcg ggtggaatcc gatccgcaca tgaggcggaa ggtttccagc ttgagcgggt 21720 acggctcccg gtgcgagctg aaatagtcga acatccgtcg ggccgtcggc gacagcttgc 21780 ggtacttctc ccatatgaat ttcgtgtagt ggtcgccagc aaacagcacg acgatttcct 21840 cgtcgatcag gacctggcaa cgggacgttt tcttgccacg gtccaggacg cggaagcggt 21900 gcagcagcga caccgattcc aggtgcccaa cgcggtcgga cgtgaagccc atcgccgtcg 21960 cctgtaggcg cgacaggcat tcctcggcct tcgtgtaata ccggccattg atcgaccagc 22020 ccaggtcctg gcaaagctcg tagaacgtga aggtgatcgg ctcgccgata ggggtgcgct 22080 tcgcgtactc caacacctgc tgccacacca gttcgtcatc gtcggcccgc agctcgacgc 22140 cggtgtaggt gatcttcacg tccttgttga cgtggaaaat gaccttgttt tgcagcgcct 22200 cgcgcgggat tttcttgttg cgcgtggtga acagggcaga gcgggccgtg tcgtttggca 22260 tcgctcgcat cgtgtccggc cacggcgcaa tatcgaacaa ggaaagctgc atttccttga 22320 tctgctgctt cgtgtgtttc agcaacgcgg cctgcttggc ctcgctgacc tgttttgcca 22380 ggtcctcgcc ggcggttttt cgcttcttgg tcgtcatagt tcctcgcgtg tcgatggtca 22440 tcgacttcgc caaacctgcc gcctcctgtt cgagacgacg cgaacgctcc acggcggccg 22500 atggcgcggg cagggcaggg ggagccagtt gcacgctgtc gcgctcgatc ttggccgtag 22560 cttgctggac catcgagccg acggactgga aggtttcgcg gggcgcacgc atgacggtgc 22620 ggcttgcgat ggtttcggca tcctcggcgg aaaaccccgc gtcgatcagt tcttgcctgt 22680 atgccttccg gtcaaacgtc cgattcattc accctccttg cgggattgcc ccgactcacg 22740 ccggggcaat gtgcccttat tcctgatttg acccgcctgg tgccttggtg tccagataat 22800 ccaccttatc ggcaatgaag tcggtcccgt agaccgtctg gccgtccttc tcgtacttgg 22860 tattccgaat cttgccctgc acgaatacca gcgacccctt gcccaaatac ttgccgtggg 22920 cctcggcctg agagccaaaa cacttgatgc ggaagaagtc ggtgcgctcc tgcttgtcgc 22980 cggcatcgtt gcgccactct tcattaaccg ctatatcgaa aattgcttgc ggcttgttag 23040 aattgccatg acgtacctcg gtgtcacggg taagattacc gataaactgg aactgattat 23100 ggctcatatc gaaagtctcc ttgagaaagg agactctagt ttagctaaac attggttccg 23160 ctgtcaagaa ctttagcggc taaaattttg cgggccgcga ccaaaggtgc gaggggcggc 23220 ttccgctgtg tacaaccaga tatttttcac caacatcctt cgtctgctcg atgagcgggg 23280 catgacgaaa catgagctgt cggagagggc aggggtttca atttcgtttt tatcagactt 23340 aaccaacggt aaggccaacc cctcgttgaa ggtgatggag gccattgccg acgccctgga 23400 aactccccta cctcttctcc tggagtccac cgaccttgac cgcgaggcac tcgcggagat 23460 tgcgggtcat cctttcaaga gcagcgtgcc gcccggatac gaacgcatca gtgtggtttt 23520 gccgtcacat aaggcgttta tcgtaaagaa atggggcgac gacacccgaa aaaagctgcg 23580 tggaaggctc tgacgccaag ggttagggct tgcacttcct tctttagccg ctaaaacggc 23640 cccttctctg cgggccgtcg gctcgcgcat catatcgaca tcctcaacgg aagccgtgcc 23700 gcgaatggca tcgggcgggt gcgctttgac agttgttttc tatcagaacc cctacgtcgt 23760 gcggttcgat tagctgtttg tcttgcaggc taaacacttt cggtatatcg tttgcctgtg 23820 cgataatgtt gctaatgatt tgttgcgtag gggttactga aaagtgagcg ggaaagaaga 23880 gtttcagacc atcaaggagc gggccaagcg caagctggaa cgcgacatgg gtgcggacct 23940 gttggccgcg ctcaacgacc cgaaaaccgt tgaagtcatg ctcaacgcgg acggcaaggt 24000 gtggcacgaa cgccttggcg agccgatgcg gtacatctgc gacatgcggc ccagccagtc 24060 gcaggcgatt atagaaacgg tggccggatt ccacggcaaa gaggtcacgc ggcattcgcc 24120 catcctggaa ggcgagttcc ccttggatgg cagccgcttt gccggccaat tgccgccggt 24180 cgtggccgcg ccaacctttg cgatccgcaa gcgcgcggtc gccatcttca cgctggaaca 24240 gtacgtcgag gcgggcatca tgacccgcga gcaatacgag gtcattaaaa gcgccgtcgc 24300 ggcgcatcga aacatcctcg tcattggcgg tactggctcg ggcaagacca cgctcgtcaa 24360 cgcgatcatc aatgaaatgg tcgccttcaa cccgtctgag cgcgtcgtca tcatcgagga 24420 caccggcgaa atccagtgcg ccgcagagaa cgccgtccaa taccacacca gcatcgacgt 24480 ctcgatgacg ctgctgctca agacaacgct gcgtatgcgc cccgaccgca tcctggtcgg 24540 tgaggtacgt ggccccgaag cccttgatct gttgatggcc tggaacaccg ggcatgaagg 24600 aggtgccgcc accctgcacg caaacaaccc caaagcgggc ctgagccggc tcgccatgct 24660 tatcagcatg cacccggatt caccgaaacc cattgagccg ctgattggcg aggcggttca 24720 tgtggtcgtc catatcgcca ggacccctag cggccgtcga gtgcaagaaa ttctcgaagt 24780 tcttggttac gagaacggcc agtacatcac caaaaccctg taaggagtat ttccaatgac 24840 aacggctgtt ccgttccgtc tgaccatgaa tcgcggcatt ttgttctacc ttgccgtgtt 24900 cttcgttctc gctctcgcgt tatccgcgca tccggcgatg gcctcggaag gcaccggcgg 24960 cagcttgcca tatgagagct ggctgacgaa cctgcgcaac tccgtaaccg gcccggtggc 25020 cttcgcgctg tccatcatcg gcatcgtcgt cgccggcggc gtgctgatct tcggcggcga 25080 actcaacgcc ttcttccgaa ccctgatctt cctggttctg gtgatggcgc tgctggtcgg 25140 cgcgcagaac gtgatgagca ccttcttcgg tcgtggtgcc gaaatcgcgg ccctcggcaa 25200 cggggcgctg caccaggtgc aagtcgcggc ggcggatgcc gtgcgtgcgg tagcggctgg 25260 acggctcgcc taatcatggc tctgcgcacg atccccatcc gtcgcgcagg caaccgagaa 25320 aacctgttca tgggtggtga tcgtgaactg gtgatgttct cgggcctgat ggcgtttgcg 25380 ctgattttca gcgcccaaga gctgcgggcc accgtggtcg gtctgatcct gtggttcggg 25440 gcgctctatg cgttccgaat catggcgaag gccgatccga agatgcggtt cgtgtacctg 25500 cgtcaccgcc ggtacaagcc gtattacccg gcccgctcga ccccgttccg cgagaacacc 25560 aatagccaag ggaagcaata ccgatgatcc aagcaattgc gattgcaatc gcgggcctcg 25620 gcgcgcttct gttgttcatc ctctttgccc gcatccgcgc ggtcgatgcc gaactgaaac 25680 tgaaaaagca tcgttccaag gacgccggcc tggccgatct gctcaactac gccgctgtcg 25740 tcgatgacgg cgtaatcgtg ggcaagaacg gcagctttat ggctgcctgg ctgtacaagg 25800 gcgatgacaa cgcaagcagc accgaccagc agcgcgaagt agtgtccgcc cgcatcaacc 25860 aggccctcgc gggcctggga agtgggtgga tgatccatgt ggacgccgtg cggcgtcctg 25920 ctccgaacta cgcggagcgg ggcctgtcgg cgttccctga ccgtctgacg gcagcgattg 25980 aagaagagcg ctcggtcttg ccttgctcgt cggtgatgta cttcaccagc tccgcgaagt 26040 cgctcttctt gatggagcgc atggggacgt gcttggcaat cacgcgcacc ccccggccgt 26100 tttagcggct aaaaaagtca tggctctgcc ctcgggcgga ccacgcccat catgaccttg 26160 ccaagctcgt cctgcttctc ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg 26220 aaccgcgccg tgcgcgggtc gtcggtgagc cagagtttca gcaggccgcc caggcggccc 26280 aggtcgccat tgatgcgggc cagctcgcgg acgtgctcat agtccacgac gcccgtgatt 26340 ttgtagccct ggccgacggc cagcaggtag gccgacaggc tcatgccggc cgccgccgcc 26400 ttttcctcaa tcgctcttcg ttcgtctgga aggcagtaca ccttgatagg tgggctgccc 26460 ttcctggttg gcttggtttc atcagccatc cgcttgccct catctgttac gccggcggta 26520 gccggccagc ctcgcagagc aggattcccg ttgagcaccg ccaggtgcga ataagggaca 26580 gtgaagaagg aacacccgct cgcgggtggg cctacttcac ctatcctgcc cggctgacgc 26640 cgttggatac accaaggaaa gtctacacga accctttggc aaaatcctgt atatcgtgcg 26700 aaaaaggatg gatataccga aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg 26760 gaaaagcgct gcttccctgc tgttttgtgg aatatctacc gactggaaac aggcaaatgc 26820 aggaaattac tgaactgagg ggacaggcga gagacgatgc caaagagcta caccgacgag 26880 ctggccgagt gggttgaatc ccgcgcggcc aagaagcgcc ggcgtgatga ggctgcggtt 26940 gcgttcctgg cggtgagggc ggatgtcgag gcggcgttag cgtccggcta tgcgctcgtc 27000 accatttggg agcacatgcg ggaaacgggg aaggtcaagt tctcctacga gacgttccgc 27060 tcgcacgcca ggcggcacat caaggccaag cccgccgatg tgcccgcacc gcaggccaag 27120 gctgcggaac ccgcgccggc acccaagacg ccggagccac ggcggccgaa gcaggggggc 27180 aaggctgaaa agccggcccc cgctgcggcc ccgaccggct tcaccttcaa cccaacaccg 27240 gacaaaaagg atctactgta atggcgaaaa ttcacatggt tttgcagggc aagggcgggg 27300 tcggcaagtc ggccatcgcc gcgatcattg cgcagtacaa gatggacaag gggcagacac 27360 ccttgtgcat cgacaccgac ccggtgaacg cgacgttcga gggctacaag gccctgaacg 27420 tccgccggct gaacatcatg gccggcgacg aaattaactc gcgcaacttc gacaccctgg 27480 tcgagctgat tgcgccgacc aaggatgacg tggtgatcga caacggtgcc agctcgttcg 27540 tgcctctgtc gcattacctc atcagcaacc aggtgccggc tctgctgcaa gaaatggggc 27600 atgagctggt catccatacc gtcgtcaccg gcggccaggc tctcctggac acggtgagcg 27660 gcttcgccca gctcgccagc cagttcccgg ccgaagcgct tttcgtggtc tggctgaacc 27720 cgtattgggg gcctatcgag catgagggca agagctttga gcagatgaag gcgtacacgg 27780 ccaacaaggc ccgcgtgtcg tccatcatcc agattccggc cctcaaggaa gaaacctacg 27840 gccgcgattt cagcgacatg ctgcaagagc ggctgacgtt cgaccaggcg ctggccgatg 27900 aatcgctcac gatcatgacg cggcaacgcc tcaagatcgt gcggcgcggc ctgtttgaac 27960 agctcgacgc ggcggccgtg ctatgagcga ccagattgaa gagctgatcc gggagattgc 28020 ggccaagcac ggcatcgccg tcggccgcga cgacccggtg ctgatcctgc ataccatcaa 28080 cgcccggctc atggccgaca gtgcggccaa gcaagaggaa atccttgccg cgttcaagga 28140 agagctggaa gggatcgccc atcgttgggg cgaggacgcc aaggccaaag cggagcggat 28200 gctgaacgcg gccctggcgg ccagcaagga cgcaatggcg aaggtaatga aggacagcgc 28260 cgcgcaggcg gccgaagcga tccgcaggga aatcgacgac ggccttggcc gccagctcgc 28320 ggccaaggtc gcggacgcgc ggcgcgtggc gatgatgaac atgatcgccg gcggcatggt 28380 gttgttcgcg gccgccctgg tggtgtgggc ctcgttatga atcgcagagg cgcagatgaa 28440 aaagcccggc gttgccgggc tttgtttttg cgttagctgg gcttgtttga caggcccaag 28500 ctctgactgc gcccgcgctc gcgctcctgg gcctgtttct tctcctgctc ctgcttgcgc 28560 atcagggcct ggtgccgtcg ggctgcttca cgcatcgaat cccagtcgcc ggccagctcg 28620 ggatgctccg cgcgcatctt gcgcgtcgcc agttcctcga tcttgggcgc gtgaatgccc 28680 atgccttcct tgatttcgcg caccatgtcc agccgcgtgt gcagggtctg caagcgggct 28740 tgctgttggg cctgctgctg ctgccaggcg gcctttgtac gcggcaggga cagcaagccg 28800 ggggcattgg actgtagctg ctgcaaacgc gcctgctgac ggtctacgag ctgttctagg 28860 cggtcctcga tgcgctccac ctggtcatgc tttgcctgca cgtagagcgc aagggtctgc 28920 tggtaggtct gctcgatggg cgcggattct aagagggcct gctgttccgt ctcggcctcc 28980 tgggccgcct gtagcaaatc ctcgccgctg ttgccgctgg actgctttac tgccggggac 29040 tgctgttgcc ctgctcgcgc cgtcgtcgca gttcggcttg cccccactcg attgactgct 29100 tcatttcgag ccgcagcgat gcgatctcgg attgcgtcaa cggacggggc agcgcggagg 29160 tgtccggctt ctccttgggt gagtcggtcg atgccatagc caaaggtttc cttccaaaat 29220 gcgtccattg ctggaccgtg tttctcattg atgcccgcaa gcatcttcgg cttgaccgcc 29280 aggtcaagcg cgccttcatg ggcggtcatg acggacgccg ccatgacctt gccgccgttg 29340 ttctcgatgt agccgcgtaa tgaggcaatg gtgccgccca tcgtcagcgt gtcatcgaca 29400 acgatgtact tctggccggg gatcacctcc ccctcgaaag tcgggttgaa cgccaggcga 29460 tgatctgaac cggctccggt tcgggcgacc ttctcccgct gcacaatgtc cgtttcgacc 29520 tcaaggccaa ggcggtcggc cagaacgacc gccatcatgg ccggaatctt gttgttcccc 29580 gccgcctcga cggcgaggac tggaacgatg cggggcttgt cgtcgccgat cagcgtcttg 29640 agctgggcaa cagtgtcgtc cgaaatcagg cgctcgacca aattaagcgc cgcttccgcg 29700 tcgccctgct tcgcagcctg gtattcaggc tcgttggtca aagaaccaag gtcgccgttg 29760 cgaaccacct tcgggaagtc tccccacggt gcgcgctcgg ctctgctgta gctgctcaag 29820 acgcctccct ttttagccgc taaaactcta acgagtgcgc ccgcgactca acttgacgct 29880 ttcggcactt acctgtgcct tgccacttgc gtcataggtg atgcttttcg cactcccgat 29940 ttcaggtact ttatcgaaat ctgaccgggc gtgcattaca aagttcttcc ccacctgttg 30000 gtaaatgctg ccgctatctg cgtggacgat gctgccgtcg tggcgctgcg acttatcggc 30060 cttttgggcc atatagatgt tgtaaatgcc aggtttcagg gccccggctt tatctacctt 30120 ctggttcgtc catgcgcctt ggttctcggt ctggacaatt ctttgcccat tcatgaccag 30180 gaggcggtgt ttcattgggt gactcctgac ggttgcctct ggtgttaaac gtgtcctggt 30240 cgcttgccgg ctaaaaaaaa gccgacctcg gcagttcgag gccggctttc cctagagccg 30300 ggcgcgtcaa ggttgttcca tctattttag tgaactgcgt tcgatttatc agttactttc 30360 ctcccgcttt gtgtttcctc ccactcgttt ccgcgtctag ccgacccctc aacatagcgg 30420 cctcttcttg ggctgccttt gcctcttgcc gcgcttcgtc acgctcggct tgcaccgtcg 30480 taaagcgctc ggcctgcctg gccgcctctt gcgccgccaa cttcctttgc tcctggtggg 30540 cctcggcgtc ggcctgcgcc ttcgctttca ccgctgccaa ctccgtgcgc aaactctccg 30600 cttcgcgcct ggtggcgtcg cgctcgccgc gaagcgcctg catttcctgg ttggccgcgt 30660 ccagggtctt gcggctctct tctttgaatg cgcgggcgtc ctggtgagcg tagtccagct 30720 cggcgcgcag ctcctgcgct cgacgctcca cctcgtcggc ccgctgcgtc gccagcgcgg 30780 cccgctgctc ggctcctgcc agggcggtgc gtgcttcggc cagggcttgc cgctggcgtg 30840 cggccagctc ggccgcctcg gcggcctgct gctctagcaa tgtaacgcgc gcctgggctt 30900 cttccagctc gcgggcctgc gcctcgaagg cgtcggccag ctccccgcgc acggcttcca 30960 actcgttgcg ctcacgatcc cagccggctt gcgctgcctg caacgattca ttggcaaggg 31020 cctgggcggc ttgccagagg gcggccacgg cctggttgcc ggcctgctgc accgcgtccg 31080 gcacctggac tgccagcggg gcggcctgcg ccgtgcgctg gcgtcgccat tcgcgcatgc 31140 cggcgctggc gtcgttcatg ttgacgcggg cggccttacg cactgcatcc acggtcggga 31200 agttctcccg gtcgccttgc tcgaacagct cgtccgcagc cgcaaaaatg cggtcgcgcg 31260 tctctttgtt cagttccatg ttggctccgg taattggtaa gaataataat actcttacct 31320 accttatcag cgcaagagtt tagctgaaca gttctcgact taacggcagg ttttttagcg 31380 gctgaagggc aggcaaaaaa agccccgcac ggtcggcggg ggcaaagggt cagcgggaag 31440 gggattagcg ggcgtcgggc ttcttcatgc gtcggggccg cgcttcttgg gatggagcac 31500 gacgaagcgc gcacgcgcat cgtcctcggc cctatcggcc cgcgtcgcgg tcaggaactt 31560 gtcgcgcgct aggtcctccc tggtgggcac caggggcatg aactcggcct gctcgatgta 31620 ggtccactcc atgaccgcat cgcagtcgag gccgcgttcc ttcaccgtct cttgcaggtc 31680 gcggtacgcc cgctcgttga gcggctggta acgggccaat tggtcgtaaa tggctgtcgg 31740 ccatgagcgg cctttcctgt tgagccagca gccgacgacg aagccggcaa tgcaggcccc 31800 tggcacaacc aggccgacgc cgggggcagg ggatggcagc agctcgccaa ccaggaaccc 31860 cgccgcgatg atgccgatgc cggtcaacca gcccttgaaa ctatccggcc ccgaaacacc 31920 cctgcgcatt gcctggatgc tgcgccggat agcttgcaac atcaggagcc gtttcttttg 31980 ttcgtcagtc atggtccgcc ctcaccagtt gttcgtatcg gtgtcggacg aactgaaatc 32040 gcaagagctg ccggtatcgg tccagccgct gtccgtgtcg ctgctgccga agcacggcga 32100 ggggtccgcg aacgccgcag acggcgtatc cggccgcagc gcatcgccca gcatggcccc 32160 ggtcagcgag ccgccggcca ggtagcccag catggtgctg ttggtcgccc cggccaccag 32220 ggccgacgtg acgaaatcgc cgtcattccc tctggattgt tcgctgctcg gcggggcagt 32280 gcgccgcgcc ggcggcgtcg tggatggctc gggttggctg gcctgcgacg gccggcgaaa 32340 ggtgcgcagc agctcgttat cgaccggctg cggcgtcggg gccgccgcct tgcgctgcgg 32400 tcggtgttcc ttcttcggct cgcgcagctt gaacagcatg atcgcggaaa ccagcagcaa 32460 cgccgcgcct acgcctcccg cgatgtagaa cagcatcgga ttcattcttc ggtcctcctt 32520 gtagcggaac cgttgtctgt gcggcgcggg tggcccgcgc cgctgtcttt ggggatcagc 32580 cctcgatgag cgcgaccagt ttcacgtcgg caaggttcgc ctcgaactcc tggccgtcgt 32640 cctcgtactt caaccaggca tagccttccg ccggcggccg acggttgagg ataaggcggg 32700 cagggcgctc gtcgtgctcg acctggacga tggccttttt cagcttgtcc gggtccggct 32760 ccttcgcgcc cttttccttg gcgtccttac cgtcctggtc gccgtcctcg ccgtcctggc 32820 cgtcgccggc ctccgcgtca cgctcggcat cagtctggcc gttgaaggca tcgacggtgt 32880 tgggatcgcg gcccttctcg tccaggaact cgcgcagcag cttgaccgtg ccgcgcgtga 32940 tttcctgggt gtcgtcgtca agccacgcct cgacttcctc cgggcgcttc ttgaaggccg 33000 tcaccagctc gttcaccacg gtcacgtcgc gcacgcggcc ggtgttgaac gcatcggcga 33060 tcttctccgg caggtccagc agcgtgacgt gctgggtgat gaacgccggc gacttgccga 33120 tttccttggc gatatcgcct ttcttcttgc ccttcgccag ctcgcggcca atgaagtcgg 33180 caatttcgcg cggggtcagc tcgttgcgtt gcaggttctc gataacctgg tcggcttcgt 33240 tgtagtcgtt gtcgatgaac gccgggatgg acttcttgcc ggcccacttc gagccacggt 33300 agcggcgggc gccgtgattg atgatatagc ggcccggctg ctcctggttc tcgcgcaccg 33360 aaatgggtga cttcaccccg cgctctttga tcgtggcacc gatttccgcg atgctctccg 33420 gggaaaagcc ggggttgtcg gccgtccgcg gctgatgcgg atcttcgtcg atcaggtcca 33480 ggtccagctc gatagggccg gaaccgccct gagacgccgc aggagcgtcc aggaggctcg 33540 acaggtcgcc gatgctatcc aaccccaggc cggacggctg cgccgcgcct gcggcttcct 33600 gagcggccgc agcggtgttt ttcttggtgg tcttggcttg agccgcagtc attgggaaat 33660 ctccatcttc gtgaacacgt aatcagccag ggcgcgaacc tctttcgatg ccttgcgcgc 33720 ggccgttttc ttgatcttcc agaccggcac accggatgcg agggcatcgg cgatgctgct 33780 gcgcaggcca acggtggccg gaatcatcat cttggggtac gcggccagca gctcggcttg 33840 gtggcgcgcg tggcgcggat tccgcgcatc gaccttgctg ggcaccatgc caaggaattg 33900 cagcttggcg ttcttctggc gcacgttcgc aatggtcgtg accatcttct tgatgccctg 33960 gatgctgtac gcctcaagct cgatggggga cagcacatag tcggccgcga agagggcggc 34020 cgccaggccg acgccaaggg tcggggccgt gtcgatcagg cacacgtcga agccttggtt 34080 cgccagggcc ttgatgttcg ccccgaacag ctcgcgggcg tcgtccagcg acagccgttc 34140 ggcgttcgcc agtaccgggt tggactcgat gagggcgagg cgcgcggcct ggccgtcgcc 34200 ggctgcgggt gcggtttcgg tccagccgcc ggcagggaca gcgccgaaca gcttgcttgc 34260 atgcaggccg gtagcaaagt ccttgagcgt gtaggacgca ttgccctggg ggtccaggtc 34320 gatcacggca acccgcaagc cgcgctcgaa aaagtcgaag gcaagatgca caagggtcga 34380 agtcttgccg acgccgcctt tctggttggc cgtgaccaaa gttttcatcg tttggtttcc 34440 tgttttttct tggcgtccgc ttcccacttc cggacgatgt acgcctgatg ttccggcaga 34500 accgccgtta cccgcgcgta cccctcgggc aagttcttgt cctcgaacgc ggcccacacg 34560 cgatgcaccg cttgcgacac tgcgcccctg gtcagtccca gcgacgttgc gaacgtcgcc 34620 tgtggcttcc catcgactaa gacgccccgc gctatctcga tggtctgctg ccccacttcc 34680 agcccctgga tcgcctcctg gaactggctt tcggtaagcc gtttcttcat ggataacacc 34740 cataatttgc tccgcgcctt ggttgaacat agcggtgaca gccgccagca catgagagaa 34800 gtttagctaa acatttctcg cacgtcaaca cctttagccg ctaaaactcg tccttggcgt 34860 aacaaaacaa aagcccggaa accgggcttt cgtctcttgc cgcttatggc tctgcacccg 34920 gctccatcac caacaggtcg cgcacgcgct tcactcggtt gcggatcgac actgccagcc 34980 caacaaagcc ggttgccgcc gccgccagga tcgcgccgat gatgccggcc acaccggcca 35040 tcgcccacca ggtcgccgcc ttccggttcc attcctgctg gtactgcttc gcaatgctgg 35100 acctcggctc accataggct gaccgctcga tggcgtatgc cgcttctccc cttggcgtaa 35160 aacccagcgc cgcaggcggc attgccatgc tgcccgccgc tttcccgacc acgacgcgcg 35220 caccaggctt gcggtccaga ccttcggcca cggcgagctg cgcaaggaca taatcagccg 35280 ccgacttggc tccacgcgcc tcgatcagct cttgcactcg cgcgaaatcc ttggcctcca 35340 cggccgccat gaatcgcgca cgcggcgaag gctccgcagg gccggcgtcg tgatcgccgc 35400 cgagaatgcc cttcaccaag ttcgacgaca cgaaaatcat gctgacggct atcaccatca 35460 tgcagacgga tcgcacgaac ccgctgaatt gaacacgagc acggcacccg cgaccactat 35520 gccaagaatg cccaaggtaa aaattgccgg ccccgccatg aagtccgtga atgccccgac 35580 ggccgaagtg aagggcaggc cgccacccag gccgccgccc tcactgcccg gcacctggtc 35640 gctgaatgtc gatgccagca cctgcggcac gtcaatgctt ccgggcgtcg cgctcgggct 35700 gatcgcccat cccgttactg ccccgatccc ggcaatggca aggactgcca gcgctgccat 35760 ttttggggtg aggccgttcg cggccgaggg gcgcagcccc tggggggatg ggaggcccgc 35820 gttagcgggc cgggagggtt cgagaagggg gggcaccccc cttcggcgtg cgcggtcacg 35880 cgcacagggc gcagccctgg ttaaaaacaa ggtttataaa tattggttta aaagcaggtt 35940 aaaagacagg ttagcggtgg ccgaaaaacg ggcggaaacc cttgcaaatg ctggattttc 36000 tgcctgtgga cagcccctca aatgtcaata ggtgcgcccc tcatctgtca gcactctgcc 36060 cctcaagtgt caaggatcgc gcccctcatc tgtcagtagt cgcgcccctc aagtgtcaat 36120 accgcagggc acttatcccc aggcttgtcc acatcatctg tgggaaactc gcgtaaaatc 36180 aggcgttttc gccgatttgc gaggctggcc agctccacgt cgccggccga aatcgagcct 36240 gcccctcatc tgtcaacgcc gcgccgggtg agtcggcccc tcaagtgtca acgtccgccc 36300 ctcatctgtc agtgagggcc aagttttccg cgaggtatcc acaacgccgg cggccgcggt 36360 gtctcgcaca cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca 36420 gcccagcggc gagggcaacc agcccggtga gcgtcggaaa ggcgctggaa gccccgtagc 36480 gacgcggaga ggggcgagac aagccaaggg cgcaggctcg atgcgcagca cgacatagcc 36540 ggttctcgca aggacgagaa tttccctgcg gtgcccctca agtgtcaatg aaagtttcca 36600 acgcgagcca ttcgcgagag ccttgagtcc acgctagatg agagctttgt tgtaggtgga 36660 ccagttggtg attttgaact tttgctttgc cacggaacgg tctgcgttgt cgggaagatg 36720 cgtgatctga tccttcaact cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc 36780 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 36840 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 36900 ttgctcgac 36909 // ID AB027256; SV 2; linear; other DNA; STD; SYN; 6323 BP. XX AC AB027256; XX DT 01-JUN-1999 (Rel. 59, Created) DT 01-FEB-2005 (Rel. 82, Last updated, Version 3) XX DE Cloning vector pSB11 genes for streptomycin/spectinomycin DE nucleotydyltransferase, streptothricin acetyltransferase 3', complete cds. XX KW aadA; streptomycin/spectinomycin nucleotydyltransferase; KW streptothricin acetyltransferase 3'. XX OS Cloning vector pSB11 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-6323 RA Komari T., Kuraya Y., Murai N., Ueki J., Saito Y.; RT ; RL Submitted (10-MAY-1999) to the INSDC. RL Jun Ueki, Japan Tobacco Inc., Plant Innovation Center; 700 Higashibara, RL Iwata, Shizuoka, 438-0802, Japan (E-mail:jun.ueki@ims.jti.co.jp, RL Tel:81-538-32-7120, Fax:81-538-33-6046) XX RN [2] RX DOI; 10.1046/j.1365-313X.1996.10010165.x. RX PUBMED; 8758986. RA Komari T., Hiei Y., Saito Y., Murai N., Kumashiro T.; RT "Vectors carrying two separate T-DNAs for co-transformation of higher RT plants mediated by Agrobacterium tumefaciens and segregation of RT transformants free from selection markers"; RL Plant J. 10(1):165-174(1996). XX DR MD5; 1b56f50a5fbf58fb77cec48806df682d. XX FH Key Location/Qualifiers FH FT source 1..6323 FT /organism="Cloning vector pSB11" FT /mol_type="other DNA" FT /db_xref="taxon:94004" FT misc_feature 1..51 FT /note="multiple cloning sites" FT repeat_region 104..128 FT /note="left border repeat" FT CDS complement(1304..2092) FT /codon_start=1 FT /transl_table=11 FT /gene="aadA" FT /product="streptomycin/spectinomycin FT nucleotydyltransferase" FT /protein_id="BAA78030.1" FT /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH FT SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK FT RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF FT EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP FT VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK" FT CDS complement(2150..2674) FT /codon_start=1 FT /transl_table=11 FT /product="streptothricin acetyltransferase 3'" FT /protein_id="BAA78031.1" FT /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSPY FT RKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAHSL FT IEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNETAMY FT WYWFSGAQDDA" FT rep_origin 3400 FT /note="ori" FT misc_feature 4678..4689 FT /note="COS" FT repeat_region 6147..6171 FT /note="right border repeat" XX SQ Sequence 6323 BP; 1590 A; 1596 C; 1565 G; 1572 T; 0 other; aagcttgcat gcctcgagtc tagaggatcc ccgggtaccg agctcgaatt cagtacatta 60 aaaacgtccg caatgtgtta ttaagttgtc taagcgtcaa tttgtttaca ccacaatata 120 tcctgccacc agccagccaa cagctccccg accggcagct cggcacaaaa tcaccactcg 180 atacaggcag cccatcagtc cgggacggcg tcagcgggag agccgttgta aggcggcaga 240 ctttgctcat gttaccgatg ctattcggaa gaacggcaac taagctgccg ggtttgaaac 300 acggatgatc tcgcggaggg tagcatgttg attgtaacga tgacagagcg ttgctgcctg 360 tgatcaaata tcatctccct cgcagagatc cgaattatca gccttcttat tcatttctcg 420 cttaaccgtg acaggctgtc gatcttgaga actatgccga cataatagga aatcgctgga 480 taaagccgct gaggaagctg agtggcgcta tttctttaga agtgaacgtt gacgatcgtc 540 gaccgtaccc cgatgaatta attcggacgt acgttctgaa cacagctgga tacttacttg 600 ggcgattgtc atacatgaca tcaacaatgt acccgtttgt gtaaccgtct cttggaggtt 660 cgtatgacac tagtggttcc cctcagcttg cgactagatg ttgaggccta acattttatt 720 agagagcagg ctagttgctt agatacatga tcttcaggcc gttatctgtc agggcaagcg 780 aaaattggcc atttatgacg accaatgccc cgcagaagct cccatctttg ccgccataga 840 cgccgcgccc cccttttggg gtgtagaaca tccttttgcc agatgtggaa aagaagttcg 900 ttgtcccatt gttggcaatg acgtagtagc cggcgaaagt gcgagaccca tttgcgctat 960 atataagcct acgatttccg ttgcgactat tgtcgtaatt ggatgaacta ttatcgtagt 1020 tgctctcaga gttgtcgtaa tttgatggac tattgtcgta attgcttatg gagttgtcgt 1080 agttgcttgg agaaatgtcg tagttggatg gggagtagtc atagggaaga cgagcttcat 1140 ccactaaaac aattggcagg tcagcaagtg cctgccccga tgccatcgca agtacgaggc 1200 ttagaaccac cttcaacaga tcgcgcatag tcttccccag ctctctaacg cttgagttaa 1260 gccgcgccgc gaagcggcgt cggcttgaac gaattgttag acattatttg ccgactacct 1320 tggtgatctc gcctttcacg tagtgaacaa attcttccaa ctgatctgcg cgcgaggcca 1380 agcgatcttc ttgtccaaga taagcctgcc tagcttcaag tatgacgggc tgatactggg 1440 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta 1500 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt 1560 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag 1620 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg 1680 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa 1740 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg 1800 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc 1860 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa 1920 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg ccgccatcca 1980 ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc tcgatgacgc 2040 caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc atgatgttta 2100 actcctgaat taagccgcgc cgcgaagcgg tgtcggcttg aatgaattgt taggcgtcat 2160 cctgtgctcc cgagaaccag taccagtaca tcgctgtttc gttcgagact tgaggtctag 2220 ttttatacgt gaacaggtca atgccgccga gagtaaagcc acattttgcg tacaaattgc 2280 aggcaggtac attgttcgtt tgtgtctcta atcgtatgcc aaggagctgt ctgcttagtg 2340 cccacttttt cgcaaattcg atgagactgt gcgcgactcc tttgcctcgg tgcgtgtgcg 2400 acacaacaat gtgttcgata gaggctagat cgttccatgt tgagttgagt tcaatcttcc 2460 cgacaagctc ttggtcgatg aatgcgccat agcaagcaga gtcttcatca gagtcatcat 2520 ccgagatgta atccttccgg taggggctca cacttctggt agatagttca aagccttggt 2580 cggataggtg cacatcgaac acttcacgaa caatgaaatg gttctcagca tccaatgttt 2640 ccgccacctg ctcagggatc accgaaatct tcatatgacg cctaacgcct ggcacagcgg 2700 atcgcaaacc tggcgcggct tttggcacaa aaggcgtgac aggtttgcga atccgttgct 2760 gccacttgtt aacccttttg ccagatttgg taactataat ttatgttaga ggcgaagtct 2820 tgggtaaaaa ctggcctaaa attgctgggg atttcaggaa agtaaacatc accttccggc 2880 tcgatgtcta ttgtagatat atgtagtgta tctacttgat cgggggatct gctgcctcgc 2940 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 3000 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 3060 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt 3120 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 3180 cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac 3240 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 3300 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 3360 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 3420 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 3480 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 3540 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 3600 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 3660 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 3720 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 3780 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 3840 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 3900 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 3960 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 4020 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 4080 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 4140 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 4200 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 4260 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 4320 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 4380 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 4440 gttaatagtt tgcgcaacgt tgttgccatt gctgcagggg gggggggggg ggggttccat 4500 tgttcattcc acggacaaaa acagagaaag gaaacgacag aggccaaaaa gctcgctttc 4560 agcacctgtc gtttcctttc ttttcagagg gtattttaaa taaaaacatt aagttatgac 4620 gaagaagaac ggaaacgcct taaaccggaa aattttcata aatagcgaaa acccgcgagg 4680 tcgccgcccc gtaacctgtc ggatcaccgg aaaggacccg taaagtgata atgattatca 4740 tctacatatc acaacgtgcg tggaggccat caaaccacgt caaataatca attatgacgc 4800 aggtatcgta ttaattgatc tgcatcaact taacgtaaaa acaacttcag acaatacaaa 4860 tcagcgacac tgaatacggg gcaacctcat gtcccccccc ccccccccct gcaggcatcg 4920 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 4980 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 5040 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 5100 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 5160 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata 5220 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 5280 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 5340 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 5400 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 5460 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 5520 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 5580 cacctgacgt ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca 5640 cgaggccctt tcgtcttcaa gaattggtcg acgatcttgc tgcgttcgga tattttcgtg 5700 gagttcccgc cacagacccg gattgaaggc gagatccagc aactcgcgcc agatcatcct 5760 gtgacggaac tttggcgcgt gatgactggc caggacgtcg gccgaaagag cgacaagcag 5820 atcacgcttt tcgacagcgt cggatttgcg atcgaggatt tttcggcgct gcgctacgtc 5880 cgcgaccgcg ttgagggatc aagccacagc agcccactcg accttctagc cgacccagac 5940 gagccaaggg atctttttgg aatgctgctc cgtcgtcagg ctttccgacg tttgggtggt 6000 tgaacagaag tcattatcgc acggaatgcc aagcactccc gaggggaacc ctgtggttgg 6060 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta 6120 ttctaataaa cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat 6180 agtttaaact gaaggcggga aacgacaatc tgatcatgag cggagaatta agggagtcac 6240 gttatgaccc ccgccgatga cgcgggacaa gccgttttac gtttggaact gacagaaccg 6300 caacgttgaa ggagccactc agc 6323 // ID AB027498; SV 1; linear; other DNA; STD; SYN; 3237 BP. XX AC AB027498; XX DT 16-AUG-1999 (Rel. 60, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 3) XX DE Synthetic construct gene for Crm1-K1, complete cds. XX KW Crm1-K1. XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-3237 RA Kudo N.; RT ; RL Submitted (18-MAY-1999) to the INSDC. RL Nobuaki Kudo, The University of Tokyo, Department of Biotechnology; Yayoi RL 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan (E-mail:kudo@bio.m.u-tokyo.ac.jp, RL Tel:+81-3-5841-5124, Fax:+81-3-5841-8021) XX RN [2] RX DOI; 10.1073/pnas.96.16.9112. RX PUBMED; 10430904. RA Kudo N., Matsumori N., Taoka H., Fujiwara D., Schreiner E.P., Wolff B., RA Yoshida M., Horinouchi S.; RT "Leptomycin B inactivates CRM1/exportin 1 by covalent modification at a RT cysteine residue in the central conserved region"; RL Proc. Natl. Acad. Sci. U.S.A. 96(16):9112-9117(1999). XX RN [3] RX PUBMED; 8119981. RA Nishi K., Yoshida M., Fujiwara D., Nishikawa M., Horinouchi S., Beppu T.; RT "Leptomycin B targets a regulatory cascade of crm1, a fission yeast nuclear RT protein, involved in control of higher order chromosome structure and gene RT expression"; RL J Biol Chem 269(9):6320-6324(1994). XX DR MD5; 9ac5dfa37651e8d86d200b7b31dc9138. DR EuropePMC; PMC17741; 10430904. XX FH Key Location/Qualifiers FH FT source 1..3237 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="based in Schizosaccharomyces pombe" FT /note="cold-sensitive and leptomycin B-hypersensitive" FT /db_xref="taxon:32630" FT CDS 1..3237 FT /codon_start=1 FT /transl_table=11 FT /gene="crm1-K1" FT /product="Crm1-K1" FT /function="The cellular target protein of leptomycin B" FT /function="The receptor for the leucine-rich nuclear export FT signal" FT /note="A mutant crm1 gene that confers hyperresistance to FT leptomycin B" FT /protein_id="BAA83347.1" FT /translation="MEGILAFDRELDVALLDRVVQTFYQGVGAEQQQAQQVLTQFQAHP FT DAWSQAYSILEKSEYPQTKYIALSVLDKLITTRWKMLPKEQRLGIRNYIVAVMIKNSSD FT ETVLQQQKTFLNKLDLTLVQILKQEWPHNWPNFIPEIVQASKTNLSLCENNMIVLRLLS FT EEIFDYSAEQMTQLKTKNLKNQMCGEFAEIFQLCSQILERAQKPSLIKATLGTLLRFLN FT WIPLGYIFETNIVELITNRFLNVPDFRNVTIECLTEIASLTSQPQYNDKFVTMFNLVMT FT SVNSMLPLQTDFREAYEESSTNEQDFIQNLALFLCAFFSSHLRPLENPENQEVLLNAHS FT YLLNISRINEREIFKICLEYWSKLVAQLYEEMQQIPMSEMNPLLNLSSPTSLISSNPNM FT LANLPLRKHIYKDILSTLRLVMIENMVKPEEVLIVENDEGEIVREFVKETDTITLYKSM FT REVLVYLTHLDVVDTEIVMTEKLARIVVGTEWSWQNLNTLCWAIGSISGAMNEEMEKRF FT LVNVIKDLLGLSEMKRGKDNKAVVASNIMYVVGQYPRFLKAHWKFLKTVVNKLFEFMHE FT YHEGVQDMACDTFIKIAQKCRRHFVAQQLGETEPFINEIIRNLAKTTEDLTPQQTHTFY FT EACGYMISAQPQKHLQERLIFDLMALPNQAWENIVAQAAQNAQVLGDPQTVKILANVLK FT TNVAACTSIGSGFYPQIAKNYVDMLGLYKAVSGLISEVVAAQGNIATKTPHVRGLRTIK FT KEILKLVDAYISRAEDLELVGNTLIPALFEAVLLDYLQNVPDARDAEVLNLITTIVNQL FT SELLTDKIPLVLDAVFGCTLEMISKDFSEYPEHRAAFFQLLRAINLNCFPALLNIPAPQ FT FKLVINSIVWSFKHVSRDIQETGLNILLELINNMASMGPDVSNAFFQTYYISLLQDILY FT VLTDSDHKSGFKLQSLILARLFYLVESNQITVPLYDPSQFPQEMNNQLFLRQYIMNLLV FT TAFPHLQPIQIQEFVQTVLALNQDSIKFKLALRDFLIQLKEFGGDNAELYLEEKEQELA FT AQQKAQLEKAMTVPGMIKPVDMPTMEEEEL" FT variation 1585 FT /replace="t" FT /note="in Acc# X15482" XX SQ Sequence 3237 BP; 975 A; 669 C; 582 G; 1011 T; 0 other; atggagggca tcctggcatt cgatcgcgaa ttagacgttg cccttttgga tcgtgttgtc 60 caaacctttt accaaggtgt cggtgcagag caacaacagg ctcaacaagt cctgacccaa 120 tttcaggcac atccagatgc atggtcgcag gcttactcca tcttggaaaa atcagaatat 180 ccccaaacta aatatatcgc tctttccgtc cttgacaagc ttatcaccac tcgttggaaa 240 atgcttccca aggagcagcg attgggaatt cgcaattata ttgttgctgt tatgataaag 300 aactcgtcag acgaaactgt cctgcaacaa caaaaaactt ttcttaataa attggacctc 360 actctcgttc agattcttaa acaagaatgg cctcataatt ggcccaactt catcccggaa 420 attgtccaag ccagtaaaac aaacctttca ctttgtgaaa ataacatgat agtccttcgt 480 cttctgagtg aagaaatttt tgattactca gctgagcaaa tgactcagct caaaaccaaa 540 aatttaaaaa accaaatgtg tggcgaattc gccgagattt ttcaactatg ctcacaaatt 600 ctcgaacgtg cgcaaaaacc tagcctaatc aaggctacct tgggaacact tctccgattt 660 ttgaattgga taccccttgg ctatatattt gaaacaaaca ttgttgagtt aattacaaat 720 cgattcctaa atgttcctga ttttagaaac gttaccattg aatgtcttac cgaaatagct 780 agtcttactt ctcaacctca gtacaacgat aaatttgtta caatgtttaa ccttgtgatg 840 acctctgtta attctatgct tcctttacaa actgattttc gtgaggctta tgaagaaagt 900 agcacaaacg agcaagactt tatccaaaat ttggctctct tcctttgcgc tttctttagc 960 tctcatcttc gaccattaga aaatcctgaa aatcaagaag tccttcttaa tgctcattcc 1020 taccttctta atatctcgcg aattaacgag cgagaaattt tcaagatctg cctggaatat 1080 tggtcaaaat tagtagctca gctttatgaa gaaatgcagc aaatccccat gtctgaaatg 1140 aatccattac ttaatctttc tagtcctaca tctctcattt cttctaatcc taatatgtta 1200 gccaatcttc ctttaaggaa acatatctat aaagatatac tttcaactct tcgattggtt 1260 atgattgaaa acatggtcaa gcccgaagaa gtcctcatcg tcgaaaatga cgaaggtgaa 1320 attgttcgtg aatttgttaa agagacggat acaattaccc tttataaatc tatgcgtgag 1380 gttcttgtgt acctcactca cttggacgtg gttgatactg aaatcgttat gactgaaaaa 1440 cttgcacgta ttgttgttgg tacagaatgg tcttggcaaa atttgaatac tctttgttgg 1500 gcaatcggct caatatccgg tgctatgaat gaagaaatgg aaaagcgctt tcttgttaac 1560 gtgattaaag atttattggg ccttagtgaa atgaaacgtg gcaaagataa taaggctgtt 1620 gttgcctcta acattatgta cgttgtaggt caatatcctc gctttttaaa agctcattgg 1680 aaatttctca agaccgtcgt caataagcta tttgagttta tgcacgaata tcatgaaggt 1740 gttcaggata tggcttgtga tacattcatt aagattgctc aaaaatgtcg tcgccatttt 1800 gttgctcaac aactgggaga aacggagccg tttatcaacg aaattattcg taacttggcc 1860 aagacgactg aagatttaac tcctcaacag actcatactt tttatgaggc gtgcggttat 1920 atgatttctg ctcaacctca aaaacatctt caagaacgtc tcatttttga ccttatggcg 1980 ttacccaatc aggcctggga aaatattgtc gctcaggctg cacaaaacgc tcaagtttta 2040 ggggaccctc agaccgttaa aatattagca aatgttctaa agacaaacgt tgctgcatgt 2100 acttctattg gttccggttt ctatcctcaa attgctaaaa attatgtaga tatgcttggt 2160 ctttacaagg ctgttagcgg tttaatatca gaagtggttg ctgcccaagg aaatattgct 2220 accaaaactc cccacgtcag gggattacga actatcaaaa aagaaatttt gaaattagta 2280 gatgcttata taagtcgagc ggaagatttg gagttggtcg gaaatactct cattcctgcg 2340 cttttcgaag cagtgttgct tgattattta caaaatgttc ctgacgctcg tgatgccgaa 2400 gttcttaatt tgataaccac cattgtcaat caacttagcg aactccttac tgataagatt 2460 cctcttgttc ttgatgctgt ttttggatgc acccttgaaa tgattagtaa agatttttcg 2520 gaataccccg aacatcgtgc agcatttttt caacttttac gcgccattaa cttgaattgt 2580 ttccccgctt tgctcaacat cccggcacca caattcaaac tagtgatcaa tagtattgta 2640 tggtccttta agcatgttag tcgcgacatt caagaaaccg gacttaacat cttacttgaa 2700 cttattaata acatggcgag tatgggacct gatgtttcaa atgcattttt ccaaacttat 2760 tatattagct tactacaaga tatattgtat gttttgactg actctgatca caaatctggc 2820 tttaagcttc aaagcttaat tttggctcgg cttttctatc tggttgaatc aaaccagatt 2880 actgtacctc tctacgatcc ttctcaattc ccccaggaaa tgaacaatca attattttta 2940 agacagtata tcatgaatct actagtcaca gctttccccc atcttcaacc tatccaaatc 3000 caagaatttg tacagacagt gctagctctt aatcaggaca gcatcaaatt taaacttgct 3060 ttacgtgact ttttaattca attgaaagaa ttcggcgggg ataacgctga actttacttg 3120 gaggagaaag aacaagagtt ggctgctcaa caaaaagccc aactggagaa agctatgact 3180 gttcctggta tgatcaaacc tgtcgatatg cctactatgg aggaggaaga actatga 3237 // ID AB028139; SV 1; circular; other DNA; STD; SYN; 11207 BP. XX AC AB028139; XX DT 04-JUN-1999 (Rel. 59, Created) DT 02-OCT-2007 (Rel. 93, Last updated, Version 4) XX DE Dual-tagging gene trap vector pGT1 DNA, complete sequence. XX KW . XX OS Dual-tagging gene trap vector pGT1 OC other sequences; artificial sequences; vectors. XX OS Saccharomyces cerevisiae (baker's yeast) OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; OC Saccharomycetales; Saccharomycetaceae; Saccharomyces. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; OC Enterobacteriaceae; Escherichia. XX OS Drosophila melanogaster (fruit fly) OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; OC Neoptera; Holometabola; Diptera; Brachycera; Muscomorpha; Ephydroidea; OC Drosophilidae; Drosophila; Sophophora. XX RN [1] RP 1-11207 RA Lukacsovich T.; RT ; RL Submitted (31-MAY-1999) to the INSDC. RL Tamas Lukacsovich, University of California, Irvine, Developmental and Cell RL Biology; Room 4444, McGaugh Hall, Irvine, California 92697-2300, USA RL (E-mail:tlukacs@uci.edu, Tel:1-949-824-3226, Fax:1-949-824-3571) XX RN [2] RX PUBMED; 11156992. RA Lukacsovich T., Asztalos Z., Awano W., Baba K., Kondo S., Niwa S., RA Yamamoto D.; RT "Dual-tagging gene trap of novel genes in Drosophila melanogaster"; RL Genetics 157(2):727-742(2001). XX DR MD5; 3bba16427c57e9761c56b808dec25fbb. DR EPD; EP23036; DM_WHIT. DR FLYBASE; FBgn0003996; w. DR FLYBASE; FBgn0014445; Scer\GAL4. DR FLYBASE; FBgn0014449; Tn\neoR. XX FH Key Location/Qualifiers FH FT source 1..11207 FT /organism="Dual-tagging gene trap vector pGT1" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:474004" FT source 275..2920 FT /organism="Saccharomyces cerevisiae" FT /mol_type="other DNA" FT /db_xref="taxon:4932" FT source 3624..4418 FT /organism="Escherichia coli" FT /mol_type="other DNA" FT /db_xref="taxon:562" FT source 5409..8290 FT /organism="Drosophila melanogaster" FT /mol_type="other DNA" FT /db_xref="taxon:7227" FT CDS 275..2920 FT /codon_start=1 FT /transl_table=11 FT /gene="Gal4" FT /product="Gal4" FT /db_xref="GOA:Q76MW9" FT /db_xref="InterPro:IPR001138" FT /db_xref="InterPro:IPR005600" FT /db_xref="InterPro:IPR007219" FT /db_xref="InterPro:IPR036864" FT /db_xref="UniProtKB/TrEMBL:Q76MW9" FT /protein_id="BAA78208.1" FT /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK FT RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK FT DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDF FT MPRDALHGFDWSEEDDMSDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVN FT RLPTMITDRYTLASRSTTSRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQI FT LFNCILAIGAWCIEGESTDIDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQ FT KTNTSYNFHSFSIRMAISLGLNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRS FT IQLSQNTISFPSSVDDVQRTTTGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICA FT KKCLMICNEIEEVSRQAPKFLQMDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVL FT RDFFTNFTQKKSQLEQDQNDHQSYEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAW FT NCSYYLFNAVLVPIKTLLSNSKSNAENNETAQLLQQINTVLMLLKKLATFKIQTCEKYI FT QVLEEVCAPFLLSQCAIPLPHISYNNSNGSAIKNIVGSATIAQYPTLPEENVNNISVKY FT VSPGSVGPSPVPLKSGASFSDLVKLLSNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQL FT QSLVPLTPSALFGGANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN FT VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD FT EDTPPNPKKE" FT CDS complement(3624..4418) FT /codon_start=1 FT /transl_table=11 FT /gene="neo" FT /product="neomycine phosphotransferase" FT /db_xref="GOA:Q76MW8" FT /db_xref="InterPro:IPR002575" FT /db_xref="InterPro:IPR011009" FT /db_xref="InterPro:IPR024165" FT /db_xref="UniProtKB/TrEMBL:Q76MW8" FT /protein_id="BAA78209.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT CDS join(5409..5480,5846..6146,6221..6875,6908..7253, FT 7474..7605,7676..8290) FT /codon_start=1 FT /transl_table=11 FT /gene="mini-white" FT /product="white protein" FT /db_xref="GOA:P10090" FT /db_xref="InterPro:IPR003439" FT /db_xref="InterPro:IPR003593" FT /db_xref="InterPro:IPR005284" FT /db_xref="InterPro:IPR013525" FT /db_xref="InterPro:IPR017871" FT /db_xref="InterPro:IPR027417" FT /db_xref="UniProtKB/Swiss-Prot:P10090" FT /protein_id="BAA78210.1" FT /translation="MGQEDQELLIRGGSKHPSAEHLNNLIFEIPYHCRVTADASQSCIN FT QGFGQAKNYGTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRT FT RGLFCNERHIPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVS FT PSGMRLLNGQPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVA FT RVDQVIQELSLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLD FT SFTAHSVVQVLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDF FT FSYITLHLNSYPAWVPSVLPTTIRRTFTYRCWPLCPDGRSSPVIGSPRYGDNFAISKVA FT RDMEQLLATKNLEKPLEQPENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTT FT MVAILIGLIFLGQQLTQVGVMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARS FT RLYRCDTYFLGKTIAELPLFLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVST FT SFGYLISCASSSTSMALSVGPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEG FT LLINQWADVEPGEISCTSSNTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLA FT YLALRLRARRKE" XX SQ Sequence 11207 BP; 2998 A; 2648 C; 2565 G; 2996 T; 0 other; catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg 60 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag 120 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt 180 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagctct 240 ttctcttaca ggtcgaattg atgtgatgga tccaatgaag ctactgtctt ctatcgaaca 300 agcatgcgat atttgccgac ttaaaaagct caagtgctcc aaagaaaaac cgaagtgcgc 360 caagtgtctg aagaacaact gggagtgtcg ctactctccc aaaaccaaaa ggtctccgct 420 gactagggca catctgacag aagtggaatc aaggctagaa agactggaac agctatttct 480 actgattttt cctcgagaag accttgacat gattttgaaa atggattctt tacaggatat 540 aaaagcattg ttaacaggat tatttgtaca agataatgtg aataaagatg ccgtcacaga 600 tagattggct tcagtggaga ctgatatgcc tctaacattg agacagcata gaataagtgc 660 gacatcatca tcggaagaga gtagtaacaa aggtcaaaga cagttgactg tatcgattga 720 ctcggcagct catcatgata actccacaat tccgttggat tttatgccca gggatgctct 780 tcatggattt gattggtctg aagaggatga catgtcggat ggcttgccct tcctgaaaac 840 ggaccccaac aataatgggt tctttggcga cggttctctc ttatgtattc ttcgatctat 900 tggctttaaa ccggaaaatt acacgaactc taacgttaac aggctcccga ccatgattac 960 ggatagatac acgttggctt ctagatccac aacatcccgt ttacttcaaa gttatctcaa 1020 taattttcac ccctactgcc ctatcgtgca ctcaccgacg ctaatgatgt tgtataataa 1080 ccagattgaa atcgcgtcga aggatcaatg gcaaatcctt tttaactgca tattagccat 1140 tggagcctgg tgtatagagg gggaatctac tgatatagat gttttttact atcaaaatgc 1200 taaatctcat ttgacgagca aggtcttcga gtcaggttcc ataattttgg tgacagccct 1260 acatcttctg tcgcgatata cacagtggag gcagaaaaca aatactagct ataattttca 1320 cagcttttcc ataagaatgg ccatatcatt gggcttgaat agggacctcc cctcgtcctt 1380 cagtgatagc agcattctgg aacaaagacg ccgaatttgg tggtctgtct actcttggga 1440 gatccaattg tccctgcttt atggtcgatc catccagctt tctcagaata caatctcctt 1500 cccttcttct gtcgacgatg tgcagcgtac cacaacaggt cccaccatat atcatggcat 1560 cattgaaaca gcaaggctct tacaagtttt cacaaaaatc tatgaactag acaaaacagt 1620 aactgcagaa aaaagtccta tatgtgcaaa aaaatgcttg atgatttgta atgagattga 1680 ggaggtttcg agacaggcac caaagttttt acaaatggat atttccacca ccgctctaac 1740 caatttgttg aaggaacacc cttggctatc ctttacaaga ttcgaactga agtggaaaca 1800 gttgtctctt atcatttatg tattaagaga ttttttcact aattttaccc agaaaaagtc 1860 acaactagaa caggatcaaa atgatcatca aagttatgaa gttaaacgat gctccatcat 1920 gttaagcgat gcagcacaaa gaactgttat gtctgtaagt agctatatgg acaatcataa 1980 tgtcacccca tattttgcct ggaattgttc ttattacttg ttcaatgcag tcctagtacc 2040 cataaagact ctactctcaa actcaaaatc gaatgctgag aataacgaga ccgcacaatt 2100 attacaacaa attaacactg ttctgatgct attaaaaaaa ctggccactt ttaaaatcca 2160 gacttgtgaa aaatacattc aagtactgga agaggtatgt gcgccgtttc tgttatcaca 2220 gtgtgcaatc ccattaccgc atatcagtta taacaatagt aatggtagcg ccattaaaaa 2280 tattgtcggt tctgcaacta tcgcccaata ccctactctt ccggaggaaa atgtcaacaa 2340 tatcagtgtt aaatatgttt ctcctggctc agtagggcct tcacctgtgc cattgaaatc 2400 aggagcaagt ttcagtgatc tagtcaagct gttatctaac cgtccaccct ctcgtaactc 2460 tccagtgaca ataccaagaa gcacaccttc gcatcgctca gtcacgcctt ttctagggca 2520 acagcaacag ctgcaatcat tagtgccact gaccccgtct gctttgtttg gtggcgccaa 2580 ttttaatcaa agtgggaata ttgctgatag ctcattgtcc ttcactttca ctaacagtag 2640 caacggtccg aacctcataa caactcaaac aaattctcaa gcgctttcac aaccaattgc 2700 ctcctctaac gttcatgata acttcatgaa taatgaaatc acggctagta aaattgatga 2760 tggtaataat tcaaaaccac tgtcacctgg ttggacggac caaactgcgt ataacgcgtt 2820 tggaatcact acagggatgt ttaataccac tacaatggat gatgtatata actatctatt 2880 cgatgatgaa gataccccac caaacccaaa aaaagagtaa aatgaatcgt agatactgaa 2940 aaaccccgca agttcacttc aactgtgcat cgtgcaccat ctcaatttct ttcatttata 3000 catcgttttg ccttctttta tgtaactata ctcctctaag tttcaatctt ggccatgtaa 3060 cctctgatct atagaatttt ttaaatgact agaattaatg cccatctttt ttttggacct 3120 aaattcttca tgaaaatata ttacgagggc ttattcagaa gcttatcgat accgtcgact 3180 aaagccaaat agaaattatt cagttctggc ttaagttttt aaaagtgata ttatttattt 3240 ggttgtaacc aaccaaaaga atgtaaataa ctaatacata attatgttag ttttaagtta 3300 gcaacaaatt gattttagct atattagcta cttggttaat aaatagaata tatttattta 3360 aagataattc gtttttattg tcagggagtg agtttgctta aaaactcgtt tagatccact 3420 agaaggaccg cggctcctcg accggatcga aaggagggcg aagaactcca gcatgagatc 3480 cccgcgctgg aggatcatcc agccggcgtc ccggaaaacg attccgaagc ccaacctttc 3540 atagaaggcg gcggtggaat cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt 3600 catttcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg 3660 cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc ccattcgccg 3720 ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg gtccgccaca 3780 cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat gatattcggc 3840 aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc 3900 ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc atcctgatcg 3960 acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg 4020 aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc catgatggat 4080 actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac ttcgcccaat 4140 agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca aggaacgccc 4200 gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac 4260 aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa cacggcggca 4320 tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc cacccaagcg 4380 gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc 4440 tcttgatcag atcccctatt cagagttctc ttcttgtatt caataattac ttcttggcag 4500 atttcagtag ttgcagttga tttacttggt tgctggttac ttttaattga ttcactttaa 4560 cttgcacttt actgcagatt gtttagcttg ttcagctgcg cttgtttatt tgcttagctt 4620 tcgcttagcg acgtgttcac tttgcttgtt tgaattgaat tgtcgctccg tagacgaagc 4680 gcctctattt atactccggc gctcttttcg cgaacattcg aggcgcgctc tctcgaacca 4740 acgagagcag tatgccgttt actgtgtgac agagtgagag agcattagtg cagagaggga 4800 gagacccaaa aagaaaagag agaataacga ataacggcca gagaaatttc tcgagttttc 4860 tttctgccaa acaaatgacc taccacaata accagtttgt tttgggatct agtccctaat 4920 tctagtatgt atgtaagtta ataaaaccct tttttggaga atgtagattt aaaaaaacat 4980 attttttttt tattttttac tgcactggac atcattgaac ttatctgatc agttttaaat 5040 ttacttcgat ccaagggtat ttgaagtacc aggttctttc gattacctct cactcaaaat 5100 gacattccac tcaaagtcag cgctgtttgc ctccttctct gtccacagaa atatcgccgt 5160 ctctttcgcc gctgcgtccg ctatctcttt cgccaccgtt tgtagcgtta cctagcgtca 5220 atgtccgcct tcagttgcac tttgtcagcg gtttcgtgac gaagctccaa gcggtttacg 5280 ccatcaatta aacacaaagt gctgtgccaa aactcctctc gcttcttatt tttgtttgtt 5340 ttttgagtga ttggggtggt gattggtttt gggtgggtaa gcaggggaaa gtgtgaaaaa 5400 tcccggcaat gggccaagag gatcaggagc tattaattcg cggaggcagc aaacacccat 5460 ctgccgagca tctgaacaat gtgagtagta catgtgcata catcttaagt tcacttgatc 5520 tataggaact gcgattgcaa catcaaattg tctgcggcgt gagaactgcg acccacaaaa 5580 atcccaaacc gcaatcgcac aaacaaatag tgacacgaaa cagattattc tggtagctgt 5640 gctcgctata taagacaatt tttaagatca tatcatgatc aagacatcta aaggcattca 5700 ttttcgacta cattcttttt tacaaaaaat ataacaacca gatattttaa gctgatccta 5760 gatgcacaaa aaataaataa aagtataaac ctacttcgta ggatacttcg ttttgttcgg 5820 ggttagatga gcataacgct tgtagttgat atttgagatc ccctatcatt gcagggtgac 5880 agcggacgct tcgcagagct gcattaacca gggcttcggg caggccaaaa actacggcac 5940 gctcctgcca cccagtccgc cggaggactc cggttcaggg agcggccaac tagccgagaa 6000 cctcacctat gcctggcaca atatggacat ctttggggcg gtcaatcagc cgggctccgg 6060 atggcggcag ctggtcaacc ggacacgcgg actattctgc aacgagcgac acataccggc 6120 gcccaggaaa catttgctca agaacggtga gtttctattc gcagtcggct gatctgtgtg 6180 aaatcttaat aaagggtcca attaccaatt tgaaactcag tttgcggcgt ggcctatccg 6240 ggcgaacttt tggccgtgat gggcagttcc ggtgccggaa agacgaccct gctgaatgcc 6300 cttgcctttc gatcgccgca gggcatccaa gtatcgccat ccgggatgcg actgctcaat 6360 ggccaacctg tggacgccaa ggagatgcag gccaggtgcg cctatgtcca gcaggatgac 6420 ctctttatcg gctccctaac ggccagggaa cacctgattt tccaggccat ggtgcggatg 6480 ccacgacatc tgacctatcg gcagcgagtg gcccgcgtgg atcaggtgat ccaggagctt 6540 tcgctcagca aatgtcagca cacgatcatc ggtgtgcccg gcagggtgaa aggtctgtcc 6600 ggcggagaaa ggaagcgtct ggcattcgcc tccgaggcac taaccgatcc gccgcttctg 6660 atctgcgatg agcccacctc cggactggac tcatttaccg cccacagcgt cgtccaggtg 6720 ctgaagaagc tgtcgcagaa gggcaagacc gtcatcctga ccattcatca gccgtcttcc 6780 gagctgtttg agctctttga caagatcctt ctgatggccg agggcagggt agctttcttg 6840 ggcactccca gcgaagccgt cgacttcttt tcctagtgag ttcgatgtgt ttattaaggg 6900 tatctagcat tacattacat ctcaactcct atccagcgtg ggtgcccagt gtcctaccaa 6960 ctacaatccg gcggactttt acgtacaggt gttggccgtt gtgcccggac gggagatcga 7020 gtcccgtgat cggatcgcca agatatggcg acaattttgc tattagcaaa gtagcccggg 7080 atatggagca gttgttggcc accaaaaatt tggagaagcc actggagcag ccggagaatg 7140 ggtacaccta caaggccacc tggttcatgc agttccgggc ggtcctgtgg cgatcctggc 7200 tgtcggtgct caaggaacca ctcctcgtaa aagtgcgact tattcagaca acggtgagtg 7260 gttccagtgg aaacaaatga tataacgctt acaattcttg gaaacaaatt cgctagattt 7320 tagttagaat tgcctgattc cacacccttc ttagtttttt tcaatgagat gtatagttta 7380 tagttttgca gaaaataaat aaatttcatt taactcgcga acatgttgaa gatatgaata 7440 ttaatgagat gcgagtaaca ttttaatttg cagatggttg ccatcttgat tggcctcatc 7500 tttttgggcc aacaactcac gcaagtgggc gtgatgaata tcaacggagc catcttcctc 7560 ttcctgacca acatgacctt tcaaaacgtc tttgccacga taaatgtaag tcttgtttag 7620 aatacatttg catattaata atttactaac tttctaatga atcgattcga tttaggtgtt 7680 cacctcagag ctgccagttt ttatgaggga ggcccgaagt cgactttatc gctgtgacac 7740 atactttctg ggcaaaacga ttgccgaatt accgcttttt ctcacagtgc cactggtctt 7800 cacggcgatt gcctatccga tgatcggact gcgggccgga gtgctgcact tcttcaactg 7860 cctggcgctg gtcactctgg tggccaatgt gtcaacgtcc ttcggatatc taatatcctg 7920 cgccagctcc tcgacctcga tggcgctgtc tgtgggtccg ccggttatca taccattcct 7980 gctctttggc ggcttcttct tgaactcggg ctcggtgcca gtatacctca aatggttgtc 8040 gtacctctca tggttccgtt acgccaacga gggtctgctg attaaccaat gggcggacgt 8100 ggagccgggc gaaattagct gcacatcgtc gaacaccacg tgccccagtt cgggcaaggt 8160 catcctggag acgcttaact tctccgccgc cgatctgccg ctggactacg tgggtctggc 8220 cattctcatc gtgagcttcc gggtgctcgc atatctggct ctaagacttc gggcccgacg 8280 caaggagtag aaggtaagta gcggccgcac gtaagggtta atgttttcaa aaaaaaattc 8340 gtccgcacac aacctttcct ctcaacaagc aaacgtgcac tgaatttaag tgtatacttc 8400 ggtaagcttc ggctatcgac gggaccacct tatgttattt catcatgggc cagacccacg 8460 tagtccagcg gcagatcggc ggcggagaag ttaagcgtct ccaggatgac cttgcccgaa 8520 ctggggcacg tggtgttcga cgatgtgcag ctaatttcgc ccggctccac gtccgcccat 8580 tggttaatca gcagaccctc gttggcgtaa cggaaccatg agaggtacga caaccatttg 8640 aggtatactg gcaccgagcc cgagttcaag aagaaggcgt ttttccatag gctccgcccc 8700 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 8760 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 8820 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc 8880 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 8940 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 9000 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 9060 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 9120 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 9180 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 9240 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 9300 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 9360 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 9420 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 9480 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 9540 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 9600 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 9660 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 9720 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 9780 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 9840 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 9900 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 9960 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 10020 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat 10080 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 10140 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 10200 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 10260 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 10320 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 10380 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa 10440 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt 10500 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc 10560 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt 10620 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg 10680 caccatatgc ggtgtgaaat accgcaccga atcgcgcgga actaacgaca gtcgctccaa 10740 ggtcgtcgaa caaaaggtga atgtgttgcg gagagcgggt gggagacagc gaaagagcaa 10800 ctacgaaacg tggtgtggtg gaggtgaatt atgaagaggg cgcgcgattt gaaaagtatg 10860 tatataaaaa atatatcccg gtgttttatg tagcgataaa cgagtttttg atgtaaggta 10920 tgcaggtgtg taagtctttt ggttagaaga caaatccaaa gtctacttgt ggggatgttc 10980 gaaggggaaa tacttgtatt ctataggtca tatcttgttt ttattggcac aaatataatt 11040 acattagctt tttgaggggg caataaacag taaacacgat ggtaataatg gtaaaaaaaa 11100 aaacaagcag ttatttcgga tatatgtcgg ctactccttg cgtcgggccc gaagtcttag 11160 agccagatat gcgagcaccc ggaagctcac gatgagaatg gccagac 11207 // ID AB030249; SV 1; linear; other DNA; STD; SYN; 912 BP. XX AC AB030249; XX DT 28-JUL-1999 (Rel. 60, Created) DT 27-DEC-2006 (Rel. 90, Last updated, Version 4) XX DE Synthetic construct 3C10 scFv gene for 3C10 single chain antibody, complete DE cds. XX KW 3C10 single chain antibody; heavy chain variable domain; KW light chain variable domain. XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-912 RA Yoshikawa K., Nakayashiki N.; RT ; RL Submitted (21-JUL-1999) to the INSDC. RL Kazuhiro Yoshikawa, Aichi Medical University, Department of Pathology; RL Yazako, Karimata 21, Nagakute, Aichi 480-1195, Japan RL (E-mail:yoshikaw@amugw.aichi-med-u.ac.jp, Tel:81-561-62-3311(ex.2265), RL Fax:81-561-62-9318) XX RN [2] RX PUBMED; 11050475. RA Nakayashiki N., Yoshikawa K., Nakamura K., Hanai N., Okamoto K., RA Okamoto S., Mizuno M., Wakabayashi T., Saga S., Yoshida J., Takahashi T.; RT "Production of a single-chain variable fragment antibody recognizing type RT III mutant epidermal growth factor receptor"; RL Jpn. J. Cancer Res. 91(10):1035-1043(2000). XX RN [3] RX PUBMED; 8645581. RA Okamoto S., Yoshikawa K., Obata Y., Shibuya M., Aoki S., Yoshida J., RA Takahashi T.; RT "Monoclonal antibody against the fusion junction of a deletion-mutant RT epidermal growth factor receptor"; RL Br. J. Cancer 73(11):1366-1372(1996). XX DR MD5; 75b7ca4b9c880be1d8e95f6446c5dd7b. DR IMGT/LIGM; AB030249. XX FH Key Location/Qualifiers FH FT source 1..912 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="cloned from mouse hybridoma producing 3C10 FT monoclonal antibody recognizing a type III mutant EGF FT receptor" FT /db_xref="taxon:32630" FT CDS 1..912 FT /codon_start=1 FT /transl_table=11 FT /gene="3C10 scFv" FT /product="3C10 single chain antibody" FT /note="contains single chain Fv" FT /protein_id="BAA82610.1" FT /translation="MEIQLQQSGAELVKPGASVKLSCTGPGFNIEDYYIHWVKQRTEQG FT LEWIGRIDPENDETKYGPIFQGRATITADTSSNTVYLQLSSLTSEDTAVYYCALRGGVY FT WGPGTTLTVSSGGGGSGGGGSGGGGSHMDVVMTQSPLTLSVAIGQSASISCKSSQSLLD FT SDGKTYLNWLLQRPGQSPKRLISLVSKLDSGVPDRFTGSGSGTDFTLRISRVEAEDLGI FT YYCWQGTHFPGTFGGGTKLEIKRSGGGGSGGGGSIMRGSHHHHHHGMASMTGGQQMGRD FT LYDDDDKDPSSRSAAGTMEFEA" FT misc_feature 1..345 FT /note="encodes heavy chain variable domain" FT misc_feature 346..390 FT /note="encodes linker sequence" FT misc_feature 391..783 FT /note="encodes light chain variable domain" XX SQ Sequence 912 BP; 225 A; 209 C; 264 G; 214 T; 0 other; atggagatcc agctgcagca gtctggggca gaacttgtga agccaggggc ctcagtcaag 60 ctgtcctgca caggtcctgg cttcaacatt gaagactact atattcactg ggtgaagcag 120 aggactgaac agggcctgga atggattgga aggattgatc ctgagaatga tgaaactaaa 180 tatggcccaa tattccaggg cagggccact ataacagcag acacatcctc caacacagtc 240 tacctgcaac tcagcagcct gacatctgag gacactgccg tctattactg tgcccttcgc 300 ggtggagtct actggggccc aggaaccact ctcacagtct cctcaggagg tggtggttcc 360 ggtggtggtg gttccggagg tggtggttca catatggatg ttgtgatgac ccagtctcca 420 ctcactctat cggtcgccat tggacaatca gcctccatct cttgcaagtc aagtcagagc 480 ctcttagata gtgatggaaa gacatatttg aattggttgt tacagaggcc aggccagtct 540 ccaaagcgcc taatctctct ggtgtctaaa ctggactctg gagtccctga caggttcact 600 ggcagtggat cagggacaga tttcacactg agaatcagca gagtggaggc tgaggatttg 660 ggaatttatt attgctggca aggtacacat tttcctggga cgttcggtgg agggaccaag 720 ctggagataa aacggtcagg aggcggcgga tcaggtggcg gcggatccat tatgcggggt 780 tctcatcatc atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg 840 gatctgtacg acgatgacga taaggatccg agctcgagat ctgcagctgg taccatggaa 900 ttcgaagctt ga 912 // ID AB031036; SV 1; linear; other DNA; STD; SYN; 903 BP. XX AC AB031036; XX DT 26-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct gene for alpha-1,2-fucosyltransferase, complete cds. XX KW alpha-1,2-fucosyltransferase. XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-903 RA Endo T.; RT ; RL Submitted (10-AUG-1999) to the INSDC. RL Tetsuo Endo, Kyowa Hakko Kogyo Co., Ltd., Tokyo Research Laboratories; RL 3-6-6, Asahi-machi, Machida 194-8533, Japan RL (E-mail:endo.tetsuo@kyowa.co.jp, Tel:81-427-25-2555, Fax:81-427-26-8330) XX RN [2] RA Endo T.; RT "Alpha-1,2-fucosyltransferase"; RL Unpublished. XX DR MD5; f9ca7d1a71cf7e9b7a0bbf5b949d0a75. XX CC Sequence updated (17-Aug-1999) XX FH Key Location/Qualifiers FH FT source 1..903 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..903 FT /codon_start=1 FT /transl_table=11 FT /product="alpha-1,2-fucosyltransferase" FT /protein_id="BAB63461.1" FT /translation="MAFKVVQICGGLGNQMFQYAFAKSLQKHLNTPVLLDTTSFDWSNR FT KMQLELFPIDLPYANAKEIAIAKMQHLPKLVRDALKYIGFDRVSQEIVFEYEPKLLKPS FT RLTYFFGYFQDPRYFDAISSLIKQTFTLPPPPENNKNNNKKEEEYQRKLSLILAAKNSV FT FVHIRRGDYVGIGCQLGIDYQKKALEYMAKRVPNMELFVFCEDLKFTQNLDLGYPFTDM FT TTRDKEEEAYWDMLLMQSCKHGIIANSTYSWWAAYLMENPEKIIIGPKHWLFGHENILC FT KEWVKIESHFEVKSQKYNA" XX SQ Sequence 903 BP; 266 A; 190 C; 234 G; 213 T; 0 other; atggcgttta aagtggtgca gatttgcggc ggcctgggca accagatgtt tcagtatgcg 60 tttgcgaaaa gcctgcagaa acatctgaac accccggtgc tgctggatac caccagcttt 120 gattggagca accgcaaaat gcagctggaa ctgtttccga ttgatctgcc gtatgcgaac 180 gcgaaagaaa ttgcgattgc gaaaatgcag catctgccga aactggtgcg cgatgcgctg 240 aaatatattg gctttgatcg cgtgagccag gaaattgtgt ttgaatatga accgaaactg 300 ctgaaaccga gccgcctgac ctattttttt ggctattttc aggatccgcg ctattttgat 360 gcgattagca gcctgattaa acagaccttt accctgccgc cgccgccgga aaacaacaaa 420 aacaacaaca aaaaagaaga agaatatcag cgcaaactga gcctgattct ggcggcgaaa 480 aacagcgtgt ttgtgcatat tcgccgcggc gattatgtgg gcattggctg ccagctgggc 540 attgattatc agaaaaaagc gctggaatat atggcgaaac gcgtgccgaa catggaactg 600 tttgtgtttt gcgaagatct gaaatttacc cagaacctgg atctgggcta tccgtttacc 660 gatatgacca cccgcgataa agaagaagaa gcgtattggg atatgctgct gatgcagagc 720 tgcaaacatg gcattattgc gaacagcacc tatagctggt gggcggcgta tctgatggaa 780 aacccggaaa aaattattat tggcccgaaa cattggctgt ttggccatga aaacattctg 840 tgcaaagaat gggtgaaaat tgaaagccat tttgaagtga aaagccagaa atataacgcg 900 taa 903 // ID AB031077; SV 1; circular; other DNA; STD; SYN; 5680 BP. XX AC AB031077; XX DT 14-JAN-2000 (Rel. 62, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 3) XX DE Cloning vector pMG103 DNA, complete sequence. XX KW . XX OS Cloning vector pMG103 OC other sequences; artificial sequences; vectors. XX OS synthetic construct OC other sequences; artificial sequences. XX OS Rhodopseudomonas palustris OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Bradyrhizobiaceae; Rhodopseudomonas. OG Plasmid pMG101 XX RN [1] RP 1-5680 RA Inui M.; RT ; RL Submitted (16-AUG-1999) to the INSDC. RL Masayuki Inui, Research Institute of Innovative Technology for the Earth, RL Molecular Microbiology and Genetics Laboratory; 9-2, Kizugawadai, Soraku, RL Kizu, Kyoto 619-0292, Japan (E-mail:inui@rite.or.jp, Tel:81-774-75-2308, RL Fax:81-774-75-2321) XX RN [2] RX DOI; 10.1128/AEM.66.1.54-63.2000. RX PUBMED; 10618203. RA Inui M., Roh J.H., Zahn K., Yukawa H.; RT "Sequence analysis of the cryptic plasmid pMG101 from Rhodopseudomonas RT palustris and construction of stable cloning vectors"; RL Appl. Environ. Microbiol. 66(1):54-63(2000). XX DR MD5; 3e08647548f7452849e75d9f2cf91a00. DR EuropePMC; PMC91785; 10618203. XX FH Key Location/Qualifiers FH FT source 1..5680 FT /organism="Cloning vector pMG103" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:102152" FT source 1..27 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from cloning vector pHSG298" FT /db_xref="taxon:32630" FT source 28..3080 FT /organism="Rhodopseudomonas palustris" FT /plasmid="pMG101" FT /mol_type="other DNA" FT /note="derived from 3.0-kb XhoI-SalI fragment of pMG101" FT /db_xref="taxon:1076" FT source 3081..5680 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from cloning vector pHSG298" FT /db_xref="taxon:32630" FT CDS 3331..4146 FT /codon_start=1 FT /transl_table=11 FT /product="aminoglucoside phosphotransferase" FT /note="kanamycin-resistance" FT /protein_id="BAA89407.1" FT /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT CDS complement(4147..4455) FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ alpha" FT /protein_id="BAA89408.1" FT /translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF" FT misc_feature 4385..4441 FT /note="multiple cloning sites" XX SQ Sequence 5680 BP; 1278 A; 1452 C; 1604 G; 1346 T; 0 other; gaggtctgcc tcgtgaagaa ggtgttgctc gagcgggtca tgaacaccct gccagatcga 60 gaatcccgaa cttggcgcgc ggtattcttc aaggccctgc ctgaggtgat ggaggatttt 120 gggggcgact ggctacaagc aactggcgaa cggcgaaatc cgttcgagcg ggtggaacag 180 acgaagccaa taggaaggcc ataggcgcgt ttttatagac ttgaagcccg gaagtccgtc 240 cacacccaaa aaatcgctgt gcgaggcgct gtggctgtcc tgtgttgcct tcgatgaaag 300 gcgaaacttg ccctcatcat ggcagtcccc cctagtctgg tccttaaagc ggtcaagggc 360 gatgccggag ggagcccttg agggcttgtg gggactaggc ttctttccct ttttcaatcg 420 tctttttgaa gactgcttgt tctgaagatt ctgaaaagtg ttaggaagaa gtgttctgca 480 atgttctgta gtgttctgtt ctatatagcg ccaccaagtc tgcggtagag cgctaccaag 540 tctgcggaaa gccgccacca agtctgcggt agaagaagcg aaaacgccac caagtctgcg 600 gtagagtgcc accaagtctg cggtggcggc ggaataagcc cttgaaatta ttgagtcccg 660 ggaacagtaa ggcgccacca agtctgcgga gctaagtggt tgcgtttgcg gctacttttt 720 gcgcacacgg atcgcaggta atgccagcac ggcggggtcg gctggttcga tggaaatgcc 780 gtattgcgtg agcttgagag gcagctttgg cagcacttcc ttgatgtgtg tcagctcctc 840 gatcagctgc gcccggaacg cgcgaggccg agtgaaacct tggccaaatt gttcgctgag 900 agccttccaa ctcatggtca ccgggtggcg aatattgtga aggcgatagc ctagccagaa 960 atacaaatcg agcttgcgag ctgagcctgc gaaggctcga accgcattag cattcaaggg 1020 gagcgcacgc tgctgtaggc tctcaaagaa taccgggttg aacgtgacgg tactgggcca 1080 aagcgagcgt tggtcaagat gcggcggtag ccacacctca acctcatcga acggattgac 1140 ggttttggtt tttgccctca ccccgtccca cacactcacc cgcattgtgc aagccgccag 1200 tgcgttaatt tgttctttga aggcggtgag cgggcctttg cgtcccccgc tgtccgaaaa 1260 tcccatctcg cgcacaaagg cagtaaagct ctcggcgatt tcgatagtag gtgttttctg 1320 ccggatggct tccgaacaaa ggtgcatcat caccagtcgg gcttttggcc cgaaaggcag 1380 cggttgaagg attttcttgc cttctccgtc tcgcaggaat ccggcggaca aatccagcgc 1440 catattgccc tgcttccgtt cgaactcgcg cacttcgaga ggttgacggt cgtaaggcat 1500 accgcaaacg gcaagcacgc tgtgcaaatg ccgaacgtcg gcgggcgatg gcggtgagtt 1560 ttcgataact tcgcggatga tggaacggcg gcggcgctcg cgtggcattg cgtcaagctc 1620 agccttgcgc ttttcagcgg cggcctgttc ctcgtctcgc ttttgctggc gggtgatgag 1680 cacgctgact tgggcatcaa acgcaaactt accccgcgct ttttcaagct cggcgcgcaa 1740 atcttcatcc cggatcagcg attcgtcata cttgcccata ggcacagagt agctgatttg 1800 ctgcggtctc cctaggcgcc tgcctgccta tagggcaatt acgtacagca ttatagcgtt 1860 gtggcgttgt aacgctatag cattgcaatg ctaggacttg cgcttggccg tctcggagtg 1920 gccgcgccta gcaatcacag catcgactcc ctcgacgatg aggtcatgca cctttttgcg 1980 ctcgacaaag gcgatctctc gcagccgatc atgagccgct tggccaaata gatactggag 2040 cgcactacag gagaggcttc agatggggga ggagcggctt ttggcgcgcg catcttcacc 2100 accctggatt tcgacggagc cggagcgtca ggctccacct cgggggtgat ttctagccct 2160 tcgatgatag gggggcgctt tttcggcggg ctcatgcgac ggctttcgtt ttgagtttgt 2220 gctggatgga tctccagagt ccgcgggcct cctcggccgc cttgccgctg ggagcgtact 2280 cggtgacccc ctgccctgcc gcgatggcat cctgaaagtc agccctagaa accatcaatg 2340 gctctgcaag tgatcccagg gctgacaatc ccacggcggc ctcgctggcg cgcccgctgc 2400 gggtgatcgg ggggcactgg ttgagaacaa acaggaatgg ccgctgcagc ttgagaagcg 2460 cttggatggt cggccgggtt gcctgaatgt ctaggcgcgt cggcctagca ggcactaggg 2520 agaggtcggc tgcctgcatg gcgagagagg tggcggtgct ggcgacaccc gggcaatcga 2580 ggatggcgag ggtgtacccc ttccctgcca gcgccttcag gatttgaggg agctggggca 2640 ccttgtcagg cggaagggca tcaacggcgg gcttctcccg ctgggcctga gcccgttgat 2700 ctccccaagc tgcaagcgaa ccttgcgggt caaggtcgag ggcaatcacg gattcccctg 2760 cctctgtggc cgcgacggca atggcagcag cgagggtagt ttttcccgcg ccgcccttct 2820 gcgtgaccag agcaattgtc ttcatgcctg cactatagca ttaaggcact aaagcgtcaa 2880 agcgccatag cggcatagcg gcatagcggc atagcgctaa aatgctatag cattattaaa 2940 tacagcgcta cagcgctata atgctgcaac ggttaggacc gcaatttgcg ccccgggccg 3000 gttgcgctat cgaccagctc aattaactgc tcgggctcgg acgcgaacca cgcgaagctg 3060 ccccaagcca aggagtcgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag 3120 ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg 3180 atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa 3240 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct 3300 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc 3360 tcgaagccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc 3420 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca 3480 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc 3540 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact 3600 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta 3660 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg 3720 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct 3780 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt 3840 aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg 3900 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa 3960 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc 4020 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa 4080 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt 4140 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg 4200 gacggcggct ttgttgaata aatcgcattc gccattcagg ctgcgcaact gttgggaagg 4260 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 4320 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 4380 tgccaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggtaccg agctcgaatt 4440 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 4500 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 4560 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 4620 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggcgaa cttttgctga 4680 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa 4740 gttcaaaatc agtaaccgtc agtgccgata agttcaaagt taaacctggt gttgatacca 4800 acattgaaac gctgatcgaa aacgcgctga aaaacgctgc tgaatgtgcg agcttcttcc 4860 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 4920 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 4980 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 5040 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 5100 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 5160 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 5220 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 5280 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 5340 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 5400 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 5460 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 5520 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 5580 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 5640 ttttctacgg ggtctgacgc tcagtggaac gatccgtcga 5680 // ID AB031078; SV 1; circular; other DNA; STD; SYN; 5680 BP. XX AC AB031078; XX DT 14-JAN-2000 (Rel. 62, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 3) XX DE Cloning vector pMG105 DNA, complete sequence. XX KW . XX OS Cloning vector pMG105 OC other sequences; artificial sequences; vectors. XX OS synthetic construct OC other sequences; artificial sequences. XX OS Rhodopseudomonas palustris OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; OC Bradyrhizobiaceae; Rhodopseudomonas. OG Plasmid pMG101 XX RN [1] RP 1-5680 RA Inui M.; RT ; RL Submitted (16-AUG-1999) to the INSDC. RL Masayuki Inui, Research Institute of Innovative Technology for the Earth, RL Molecular Microbiology and Genetics Laboratory; 9-2, Kizugawadai, Soraku, RL Kizu, Kyoto 619-0292, Japan (E-mail:inui@rite.or.jp, Tel:81-774-75-2308, RL Fax:81-774-75-2321) XX RN [2] RX DOI; 10.1128/AEM.66.1.54-63.2000. RX PUBMED; 10618203. RA Inui M., Roh J.H., Zahn K., Yukawa H.; RT "Sequence analysis of the cryptic plasmid pMG101 from Rhodopseudomonas RT palustris and construction of stable cloning vectors"; RL Appl. Environ. Microbiol. 66(1):54-63(2000). XX DR MD5; d1b4dc8dd7709505df3fd17a72e8a03b. DR EuropePMC; PMC91785; 10618203. XX FH Key Location/Qualifiers FH FT source 1..5680 FT /organism="Cloning vector pMG105" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:102153" FT source 1..27 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from cloning vector pHSG299" FT /db_xref="taxon:32630" FT source 28..3080 FT /organism="Rhodopseudomonas palustris" FT /plasmid="pMG101" FT /mol_type="other DNA" FT /note="derived from 3.0-kb XhoI-SalI fragment of pMG101" FT /db_xref="taxon:1076" FT source 3081..5680 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from cloning vector pHSG299" FT /db_xref="taxon:32630" FT CDS 3331..4146 FT /codon_start=1 FT /transl_table=11 FT /product="aminoglucoside phosphotransferase" FT /note="kanamycin-resistance" FT /protein_id="BAA89409.1" FT /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT CDS complement(4147..4455) FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ alpha" FT /protein_id="BAA89410.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGECDLFNKAAVPSSQRNALPVLQPINQF" FT misc_feature 4382..4438 FT /note="multiple cloning sites" XX SQ Sequence 5680 BP; 1278 A; 1450 C; 1606 G; 1346 T; 0 other; gaggtctgcc tcgtgaagaa ggtgttgctc gagcgggtca tgaacaccct gccagatcga 60 gaatcccgaa cttggcgcgc ggtattcttc aaggccctgc ctgaggtgat ggaggatttt 120 gggggcgact ggctacaagc aactggcgaa cggcgaaatc cgttcgagcg ggtggaacag 180 acgaagccaa taggaaggcc ataggcgcgt ttttatagac ttgaagcccg gaagtccgtc 240 cacacccaaa aaatcgctgt gcgaggcgct gtggctgtcc tgtgttgcct tcgatgaaag 300 gcgaaacttg ccctcatcat ggcagtcccc cctagtctgg tccttaaagc ggtcaagggc 360 gatgccggag ggagcccttg agggcttgtg gggactaggc ttctttccct ttttcaatcg 420 tctttttgaa gactgcttgt tctgaagatt ctgaaaagtg ttaggaagaa gtgttctgca 480 atgttctgta gtgttctgtt ctatatagcg ccaccaagtc tgcggtagag cgctaccaag 540 tctgcggaaa gccgccacca agtctgcggt agaagaagcg aaaacgccac caagtctgcg 600 gtagagtgcc accaagtctg cggtggcggc ggaataagcc cttgaaatta ttgagtcccg 660 ggaacagtaa ggcgccacca agtctgcgga gctaagtggt tgcgtttgcg gctacttttt 720 gcgcacacgg atcgcaggta atgccagcac ggcggggtcg gctggttcga tggaaatgcc 780 gtattgcgtg agcttgagag gcagctttgg cagcacttcc ttgatgtgtg tcagctcctc 840 gatcagctgc gcccggaacg cgcgaggccg agtgaaacct tggccaaatt gttcgctgag 900 agccttccaa ctcatggtca ccgggtggcg aatattgtga aggcgatagc ctagccagaa 960 atacaaatcg agcttgcgag ctgagcctgc gaaggctcga accgcattag cattcaaggg 1020 gagcgcacgc tgctgtaggc tctcaaagaa taccgggttg aacgtgacgg tactgggcca 1080 aagcgagcgt tggtcaagat gcggcggtag ccacacctca acctcatcga acggattgac 1140 ggttttggtt tttgccctca ccccgtccca cacactcacc cgcattgtgc aagccgccag 1200 tgcgttaatt tgttctttga aggcggtgag cgggcctttg cgtcccccgc tgtccgaaaa 1260 tcccatctcg cgcacaaagg cagtaaagct ctcggcgatt tcgatagtag gtgttttctg 1320 ccggatggct tccgaacaaa ggtgcatcat caccagtcgg gcttttggcc cgaaaggcag 1380 cggttgaagg attttcttgc cttctccgtc tcgcaggaat ccggcggaca aatccagcgc 1440 catattgccc tgcttccgtt cgaactcgcg cacttcgaga ggttgacggt cgtaaggcat 1500 accgcaaacg gcaagcacgc tgtgcaaatg ccgaacgtcg gcgggcgatg gcggtgagtt 1560 ttcgataact tcgcggatga tggaacggcg gcggcgctcg cgtggcattg cgtcaagctc 1620 agccttgcgc ttttcagcgg cggcctgttc ctcgtctcgc ttttgctggc gggtgatgag 1680 cacgctgact tgggcatcaa acgcaaactt accccgcgct ttttcaagct cggcgcgcaa 1740 atcttcatcc cggatcagcg attcgtcata cttgcccata ggcacagagt agctgatttg 1800 ctgcggtctc cctaggcgcc tgcctgccta tagggcaatt acgtacagca ttatagcgtt 1860 gtggcgttgt aacgctatag cattgcaatg ctaggacttg cgcttggccg tctcggagtg 1920 gccgcgccta gcaatcacag catcgactcc ctcgacgatg aggtcatgca cctttttgcg 1980 ctcgacaaag gcgatctctc gcagccgatc atgagccgct tggccaaata gatactggag 2040 cgcactacag gagaggcttc agatggggga ggagcggctt ttggcgcgcg catcttcacc 2100 accctggatt tcgacggagc cggagcgtca ggctccacct cgggggtgat ttctagccct 2160 tcgatgatag gggggcgctt tttcggcggg ctcatgcgac ggctttcgtt ttgagtttgt 2220 gctggatgga tctccagagt ccgcgggcct cctcggccgc cttgccgctg ggagcgtact 2280 cggtgacccc ctgccctgcc gcgatggcat cctgaaagtc agccctagaa accatcaatg 2340 gctctgcaag tgatcccagg gctgacaatc ccacggcggc ctcgctggcg cgcccgctgc 2400 gggtgatcgg ggggcactgg ttgagaacaa acaggaatgg ccgctgcagc ttgagaagcg 2460 cttggatggt cggccgggtt gcctgaatgt ctaggcgcgt cggcctagca ggcactaggg 2520 agaggtcggc tgcctgcatg gcgagagagg tggcggtgct ggcgacaccc gggcaatcga 2580 ggatggcgag ggtgtacccc ttccctgcca gcgccttcag gatttgaggg agctggggca 2640 ccttgtcagg cggaagggca tcaacggcgg gcttctcccg ctgggcctga gcccgttgat 2700 ctccccaagc tgcaagcgaa ccttgcgggt caaggtcgag ggcaatcacg gattcccctg 2760 cctctgtggc cgcgacggca atggcagcag cgagggtagt ttttcccgcg ccgcccttct 2820 gcgtgaccag agcaattgtc ttcatgcctg cactatagca ttaaggcact aaagcgtcaa 2880 agcgccatag cggcatagcg gcatagcggc atagcgctaa aatgctatag cattattaaa 2940 tacagcgcta cagcgctata atgctgcaac ggttaggacc gcaatttgcg ccccgggccg 3000 gttgcgctat cgaccagctc aattaactgc tcgggctcgg acgcgaacca cgcgaagctg 3060 ccccaagcca aggagtcgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag 3120 ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg 3180 atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa 3240 aatctctgat gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct 3300 gcttacataa acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc 3360 tcgaagccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc 3420 gataatgtcg ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca 3480 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc 3540 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact 3600 cctgatgatg catggttact caccactgcg atccccggga aaacagcatt ccaggtatta 3660 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg 3720 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct 3780 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt 3840 aatggctggc ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg 3900 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa 3960 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc 4020 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa 4080 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt 4140 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg 4200 gacggcggct ttgttgaata aatcgcattc gccattcagg ctgcgcaact gttgggaagg 4260 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 4320 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 4380 tgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg 4440 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 4500 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 4560 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 4620 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggcgaa cttttgctga 4680 gttgaaggat cagatcacgc atcttcccga caacgcagac cgttccgtgg caaagcaaaa 4740 gttcaaaatc agtaaccgtc agtgccgata agttcaaagt taaacctggt gttgatacca 4800 acattgaaac gctgatcgaa aacgcgctga aaaacgctgc tgaatgtgcg agcttcttcc 4860 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct 4920 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg 4980 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc 5040 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga 5100 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct 5160 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg 5220 gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag 5280 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat 5340 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac 5400 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac 5460 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc 5520 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt 5580 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 5640 ttttctacgg ggtctgacgc tcagtggaac gatccgtcga 5680 // ID AB032066; SV 1; circular; other DNA; STD; SYN; 3297 BP. XX AC AB032066; XX DT 13-SEP-1999 (Rel. 61, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Cloning vector pNTT104 DNA, complete sequence. XX KW . XX OS Cloning vector pNTT104 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3297 RA Nomura N., Nagase T.; RT ; RL Submitted (31-AUG-1999) to the INSDC. RL Nobuo Nomura, Kazusa DNA Research Institute, Laboratory of Gene Structure RL 1; 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan RL (E-mail:nomura@kazusa.or.jp, URL:http://www.kazusa.or.jp, RL Tel:81-438-52-3932, Fax:81-438-52-3931) XX RN [2] RA Nomura N., Nagase T.; RT "The nucleotide sequence of the pNTT104 vector"; RL Unpublished. XX DR MD5; 4aa930d2e3cb7e007c56f661398f1d73. XX CC pNTT104 CC This sequence is derived from the pUC118 sequence mainly compiled CC by changing the polylinker sequence (sequence at the position CC between 231 and 291 of the pUC118) to the 194-bp sequence CC (sequence at the position between 231 and 426 of the pNTT104) CC containing multiple restriction enzyme recognition sites. In CC addition to that, both the ScaI and NarI sites of the pUC118 were CC eliminated by nucleotide exchanges at the positions 586 (C to T) CC and 1802 (G to A) of the pNTT104 sequence, respectively. XX FH Key Location/Qualifiers FH FT source 1..3297 FT /organism="Cloning vector pNTT104" FT /lab_host="Escherichia coli" FT /mol_type="other DNA" FT /db_xref="taxon:103691" FT misc_feature 205..462 FT /note="complementary base sequence was reported as FT Acc#:D16587" FT /note="polylinker" XX SQ Sequence 3297 BP; 789 A; 851 C; 818 G; 839 T; 0 other; agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac ggcatgcctg 240 caggcgccga cgtcatgcat gggccctcta gatctagtac tagtgatatc gatcggtccg 300 tacgtttttc agcacgcgtt aaaaaaacca tccccctgga agcttaaggc ctcgagttcg 360 aattcgcggc cgcacgtgtc catggatccc gggtcgacgg ccgcggtggc cggtaccgag 420 ctcatactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt 480 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc 540 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgcttgat gcggtatttt 600 ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc 660 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 720 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 780 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 840 tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc 900 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 960 tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat ttataaggga 1020 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 1080 attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg 1140 atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg 1200 cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt 1260 gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc 1320 tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc 1380 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc 1440 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga 1500 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 1560 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 1620 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 1680 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 1740 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 1800 aatactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 1860 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 1920 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 1980 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 2040 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 2100 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 2160 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 2220 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 2280 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 2340 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 2400 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 2460 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 2520 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 2580 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 2640 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 2700 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 2760 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 2820 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 2880 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 2940 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 3000 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 3060 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 3120 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 3180 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 3240 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag 3297 // ID AB032347; SV 1; linear; other DNA; STD; SYN; 3748 BP. XX AC AB032347; XX DT 23-SEP-1999 (Rel. 61, Created) DT 19-FEB-2009 (Rel. 99, Last updated, Version 4) XX DE Cloning vector pNI10 repA gene for hypothetical rep-protein, complete cds. XX KW . XX OS Cloning vector pNI10 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3748 RA Itoh N., Koide Y.; RT ; RL Submitted (08-SEP-1999) to the INSDC. RL Contact:Nobuya Itoh Toyama Prefectural University, Biotechnology Research RL Center; 5180 Kurokawa, Kosugi-machi, Imizu-gun, Toyama 939-0398, Japan XX RN [2] RA Itoh N., Koide Y., Nitta C., Iwata N., Usama S., Abe Y.; RT "Complete nucleotide sequence of pNI10 cryptic plasmid in Pseudomonas, the RT region required for its replication, and the construction of improved RT Escherichia and Pseudomonas shuttle vector pNU73 derived from pNI10 and RT pHSG298"; RL Unpublished. XX RN [3] RX PUBMED; 1778984. RA Itoh N., Koide Y., Fukazawa H., Hirose S., Inukai T.; RT "Novel plasmid vectors for gene cloning in Pseudomonas"; RL J. Biochem. 110(4):614-621(1991). XX DR MD5; d3cf66e65852333c21b046fdd6d93b91. XX FH Key Location/Qualifiers FH FT source 1..3748 FT /organism="Cloning vector pNI10" FT /lab_host="Pseudomonas fulva (flavida)" FT /strain="IF-4" FT /mol_type="other DNA" FT /note="pNI10 can replicate not only is Pseudomonas but also FT in some other Gram-negative enteric bacteria such as E. FT coli" FT /db_xref="taxon:103949" FT CDS 445..1860 FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="hypothetical rep-protein" FT /function="The rep-protein is required for autonomous FT replication of the plasmid in Pseudomonas" FT /protein_id="BAA84586.1" FT /translation="MKAPDSKSKTAGRGADSGSTRNLLPPQDLRAKLQANRAERYDLLA FT SARALFLHEGKREGLLHPQDWHRTAKCKWTPVAGMVGVHASREHGSAFYSGVMNCGSVW FT ACPVCAAKVQERRREEVAKAVTWADAQGLQAAMVTLTFPHYAWQQLAVLLEQQKTALKY FT LREGSPWTRFKKATGYQGLIRSLELTHGQNGWHPHTHELWFVDAGTDADTMKKTVLERW FT KTSCARAGLLDLDNADQVAAFEAHAIDVKGWCTASDYLAKQDDSRHWGVDAEIAKASTK FT AGRAKGKHPFALLTLFQDGDRKAGHRFLDYAAAMKGKRQLSWSAGLKAKVGVIEQSDEE FT VAEEQRDDADLLGHLEIEQWKLIRQAGLRAKVLDLAESNGGWVAIQHLLRGLLNGGLSM FT THSNQDLTPEDEGLPIFVSDEQEALPLPGFVTRETHRSSKAVIRQKIATAMTEDRSGND FT PAIQGKRSSNAES" XX SQ Sequence 3748 BP; 901 A; 953 C; 1111 G; 783 T; 0 other; gaattcttga tcatgcttaa aatgcgcaaa cccctgcttc tggcacgcag cgtcgtgatg 60 ccaaaatcag gcatgaccag agaggtgggg tggatgaaga ttacgatcga gctgggggat 120 tttgcgttaa aggctctgct ggccttatcg cttgcagcat ggtgtggttt ggaaagtcag 180 ccagtggctg gagcggtaat ggcgttggcc gcagtgtgga aggcgtaggg atgttcagct 240 gctcgccagc ttgcttgaaa cggtaccgtc acaagggcaa gatacgggaa ttgcagttcg 300 gcgaagatcg aaccgcagaa agcaaaaaac cgggatcaca gcgcgccaac gctcccccgg 360 ttttccacga aacaggggat agtatgacca tcatgcacgt gacttttcaa gtgctgagcc 420 ccgccaggac tggcggaggc ggctgtgaag gcaccggatt ccaagtctaa aaccgctggt 480 cggggcgccg actcgggaag tacacgaaat cttctacccc cccaggactt gcgagcgaag 540 ctgcaggcca accgggcgga gcgttatgac ctccttgcat ctgcacgggc cctgttcctc 600 catgagggga aacgggaagg tcttctgcac cctcaagact ggcaccgcac tgccaagtgc 660 aaatggacgc cagtggcagg aatggtcggc gtgcatgcct cgcgtgagca tggcagtgcc 720 ttctacagcg gtgtgatgaa ctgcggcagt gtctgggcct gtccggtctg cgcggcgaag 780 gtgcaggagc gccgccgtga agaggtggcc aaggctgtta cctgggccga tgcccaaggg 840 ctgcaagcgg cgatggtcac cctgacattc ccccactatg cctggcagca actggcggtg 900 ctcctggagc agcagaagac tgcgttgaag tacctgcgtg aaggctcgcc ctggactcgt 960 ttcaagaagg ctaccggcta ccaagggctg atccgttcgc tggaactgac ccatggccag 1020 aatggctggc acccccacac ccatgaactg tggttcgtag atgccggtac cgatgccgac 1080 accatgaaaa agaccgtgct cgagcgttgg aagaccagtt gtgcacgtgc tggtctgctc 1140 gacctggaca acgctgacca ggtcgccgcg ttcgaggcgc atgctatcga tgtgaagggt 1200 tggtgtacgg ccagcgacta cctggccaag caggatgaca gccgccactg gggggtggat 1260 gcggagattg ccaaggcctc gaccaaggcc ggccgtgcca agggaaaaca tcccttcgca 1320 ttgctcaccc tgttccagga tggtgatcgt aaggccggtc accgtttcct cgactatgcc 1380 gcggcgatga agggcaaacg tcagttgtcc tggtctgccg gactcaaggc caaggtcggg 1440 gtgatcgagc agagcgacga ggaagtggcc gaggaacagc gtgacgacgc cgacttactc 1500 ggtcacctgg agatcgagca gtggaagttg atccgccagg ctgggctgcg ggcgaaggtc 1560 ctggacctgg ctgagagcaa tggcggctgg gtggctattc agcacctgct gcggggactg 1620 ttaaacggag gtttgtcgat gacacattcg aatcaggatt tgacgccaga ggatgagggt 1680 ttgccgatct tcgtcagtga tgaacaggag gcgttgccgc tacctggatt tgttacccgg 1740 gagacgcacc gatcgagtaa ggctgtgata cggcagaaga ttgccacagc aatgacagag 1800 gacaggtcag gcaacgatcc tgcaatccaa gggaaacgaa gtagcaacgc agaaagttag 1860 gggaacgtta gggcaagaca ggtgaagtag gcccaccagc aagctggtga tccttcttca 1920 cgtcttgcct attcgcctac cggctcaagg tgagtttccg aatcggaaac tcctgacaaa 1980 agccagacaa acgcctgacg tatgccttgc aggcacgacc aatgcctgac aacagcctga 2040 caaccacctg acatgctgaa gtctcgaacc ggacgttgga ccagcagaaa aaaacacagg 2100 cacgctaagg atgtttacac actcccgtgc caaagggacg ccctttgaaa ccccgcctct 2160 acgagtcgaa aacctgacgc gcttcgcttg tcacccagac tggaccgctg gcaacaagtt 2220 gccaccagcc cagaccgggc ttatcttaat tcccatcaag caaaggcgaa aggaaatcct 2280 agaacggttg agaactggat ttttcggtgc ccgttttttg agccaaattg acaaaatgtc 2340 aattttttcg tatttttgag ctgtgagtgg tggaaggatg atccccacgt taaaaaaaga 2400 aatctccaaa ccgaaagctg tggtggaagg acgatcccca cgttaaaaaa agaaatctcc 2460 aaaccgaaag cacaaaaaag cccttctgat gaagggcttt ttttttacct gggaaaataa 2520 aaatcgatgt tgctacggaa gctagaaaac ctcttttacg atgaaaacac gcatcttgtt 2580 gaggttttgg ataaaacagg ggagcagtgg gaaccgggta aaacgcgagg ctatggagtc 2640 gtggttatcg aattgaacgg cctgcggttt ggtatccctc tgagatccca tatcaatccc 2700 agaaataagc actgctttat cacgaaggaa aggaaaggcc tggattacag caaagctgtg 2760 ctcttaccca aggatgatta catttccagc gtaccgttcc agattccgca ggatgaaatc 2820 cagaagattc gtgaggatga gcataagatc ggtaagcagt tctccaagta cgtcgagcgc 2880 tacgtgaagt acgttaaaac taaccctgag gcatccgttc tacggattga gtaccgattt 2940 agtacgttgg tcaattacca cgctgaactg ggaatctgaa ggatggaggg ggtggaagcc 3000 gggtcaaggc tcgcgtagcg acgcgtagcg gcttggcctt gaggcggctg gaatccccac 3060 acgctaaagg cgggtgtgct gggcgcttct cccagcgcac ctgctcgcgg cgagcagccc 3120 ccggaggccg ccggaggcga ggtggcctta ttgcggtttg ggaaggcttt ggaagtactg 3180 tttttgtgtg tcacgccact ggggatcgag cgcccagcgg aatacgttca aaggggcgta 3240 gagaccagag tagggccaag gcgtttgcaa aacctggaac atgcccatgt tcggggcctg 3300 ggctctagag gatgcactga ccgaatagta ttcgtcagct gctgctccaa gctgatcatc 3360 ttccttgctc aatgggctgc catatgcggc tacgtaccag ccgttttggt tgagcagggc 3420 catgtaattc tgtgctgtgc caaagaaatt tgcgggccag atttggcatg cggtacagac 3480 ggtcagcaga tgctccaagg ttaacggggt ttctccgctt tccactttgc tccaggaact 3540 agttgaagct ttcccgagaa gctggctgat gtgtgcttgc gttacgcctt tctcgacgcg 3600 tagctccctg agaagcagcc ggcaaattgt gtgaaacgat gtgcttttct ccgtcacggt 3660 gacccccagt catgtttctt taaagtagat gatgtctcct ccagggaaac aatgtctact 3720 ttaaatagat agtattcctc tggcaact 3748 // ID AB032348; SV 1; linear; other DNA; STD; SYN; 3784 BP. XX AC AB032348; XX DT 23-SEP-1999 (Rel. 61, Created) DT 19-FEB-2009 (Rel. 99, Last updated, Version 3) XX DE Cloning vector pNU73 repA, Kmr genes for rep-protein, aminoglycoside DE phosphotransferase, complete cds. XX KW . XX OS Cloning vector pNU73 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3784 RA Itoh N.; RT ; RL Submitted (08-SEP-1999) to the INSDC. RL Contact:Nobuya Itoh Toyama Prefectural University, Biotechnology Research RL Center; 5180 Kurokawa, Kosugi-machi, Imizu-gun, Toyama 939-0398, Japan XX RN [2] RA Itoh N., Koide Y., Nitta C., Iwata N., Usama S., Abe Y.; RT "Complete nucleotide sequence of pNI10 cryptic plasmid in Pseudomonas, the RT region required for its replication, and the construction of improved RT Escherichia and Pseudomonas shuttle vector pNU73 derived from pNI10 and RT pHSG298"; RL Unpublished. XX RN [3] RX PUBMED; 1778984. RA Itoh N., Koide Y., Fukazawa H., Hirose S., Inukai T.; RT "Novel plasmid vectors for gene cloning in Pseudomonas"; RL J. Biochem. 110(4):614-621(1991). XX DR MD5; 9a3b44c0522cc08aac963b714c69c2ba. DR EuropePMC; PMC165158; 12839813. XX FH Key Location/Qualifiers FH FT source 1..3784 FT /organism="Cloning vector pNU73" FT /lab_host="Pseudomonas fluva (flavida)" FT /strain="IF-4" FT /mol_type="other DNA" FT /db_xref="taxon:103950" FT CDS 146..1561 FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="rep-protein" FT /note="rep-protein from pNI10" FT /protein_id="BAA84587.1" FT /translation="MKAPDSKSKTAGRGADSGSTRNLLPPQDLRAKLQANRAERYDLLA FT SARALFLHEGKREGLLHPQDWHRTAKCKWTPVAGMVGVHASREHGSAFYSGVMNCGSVW FT ACPVCAAKVQERRREEVAKAVTWADAQGLQAAMVTLTFPHYAWQQLAVLLEQQKTALKY FT LREGSPWTRFKKATGYQGLIRSLELTHGQNGWHPHTHELWFVDAGTDADTMKKTVLERW FT KTSCARAGLLDLDNADQVAAFEAHAIDVKGWCTASDYLAKQDDSRHWGVDAEIAKASTK FT AGRAKGKHPFALLTLFQDGDRKAGHRFLDYAAAMKGKRQLSWSAGLKAKVGVIEQSDEE FT VAEEQRDDADLLGHLEIEQWKLIRQAGLRAKVLDLAESNGGWVAIQHLLRGLLNGGLSM FT THSNQDLTPEDEGLPIFVSDEQEALPLPGFVTRETHRSSKAVIRQKIATAMTEDRSGND FT PAIQGKRSSNAES" FT CDS 2208..3023 FT /codon_start=1 FT /transl_table=11 FT /gene="Kmr" FT /product="aminoglycoside phosphotransferase" FT /function="kanamycin kinase" FT /protein_id="BAA84588.1" FT /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGDFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" XX SQ Sequence 3784 BP; 931 A; 949 C; 1062 G; 842 T; 0 other; ggcgaagatc gaaccgcaga aagcaaaaaa ccgggatcac agcgcgccaa cgctcccccg 60 gttttccacg aaacagggga tagtatgacc atcatgcacg tgacttttca agtgctgagc 120 cccgccagga ctggcggagg cggctgtgaa ggcaccggat tccaagtcta aaaccgctgg 180 tcggggcgcc gactcgggaa gtacacgaaa tcttctaccc ccccaggact tgcgagcgaa 240 gctgcaggcc aaccgggcgg agcgttatga cctccttgca tctgcacggg ccctgttcct 300 ccatgagggg aaacgggaag gtcttctgca ccctcaagac tggcaccgca ctgccaagtg 360 caaatggacg ccagtggcag gaatggtcgg cgtgcatgcc tcgcgtgagc atggcagtgc 420 cttctacagc ggtgtgatga actgcggcag tgtctgggcc tgtccggtct gcgcggcgaa 480 ggtgcaggag cgccgccgtg aagaggtggc caaggctgtt acctgggccg atgcccaagg 540 gctgcaagcg gcgatggtca ccctgacatt cccccactat gcctggcagc aactggcggt 600 gctcctggag cagcagaaga ctgcgttgaa gtacctgcgt gaaggctcgc cctggactcg 660 tttcaagaag gctaccggct accaagggct gatccgttcg ctggaactga cccatggcca 720 gaatggctgg cacccccaca cccatgaact gtggttcgta gatgccggta ccgatgccga 780 caccatgaaa aagaccgtgc tcgagcgttg gaagaccagt tgtgcacgtg ctggtctgct 840 cgacctggac aacgctgacc aggtcgccgc gttcgaggcg catgctatcg atgtgaaggg 900 ttggtgtacg gccagcgact acctggccaa gcaggatgac agccgccact ggggggtgga 960 tgcggagatt gccaaggcct cgaccaaggc cggccgtgcc aagggaaaac atcccttcgc 1020 attgctcacc ctgttccagg atggtgatcg taaggccggt caccgtttcc tcgactatgc 1080 cgcggcgatg aagggcaaac gtcagttgtc ctggtctgcc ggactcaagg ccaaggtcgg 1140 ggtgatcgag cagagcgacg aggaagtggc cgaggaacag cgtgacgacg ccgacttact 1200 cggtcacctg gagatcgagc agtggaagtt gatccgccag gctgggctgc gggcgaaggt 1260 cctggacctg gctgagagca atggcggctg ggtggctatt cagcacctgc tgcggggact 1320 gttaaacgga ggtttgtcga tgacacattc gaatcaggat ttgacgccag aggatgaggg 1380 tttgccgatc ttcgtcagtg atgaacagga ggcgttgccg ctacctggat ttgttacccg 1440 ggagacgcac cgatcgagta aggctgtgat acggcagaag attgccacag caatgacaga 1500 ggacaggtca ggcaacgatc ctgcaatcca agggaaacga agtagcaacg cagaaagtta 1560 ggggaacgtt agggcaagac aggtgaagta ggcccaccag caagctggtg atccttcttc 1620 acgtcttgcc tattcgccta ccggctcaag gtgagtttcc gaatcggaaa ctcctgacaa 1680 aagccagaca aacgcctgac gtatgccttg caggcacgac caatgcctga caacagcctg 1740 acaaccacct gacatgctga agtctcgaac cggacgttgg accagcagaa aaaaacacag 1800 gcacgctaag gatgtttaca cactcccgtg ccaaagggac gccctttgaa accccgcctc 1860 tacgagtcga aaacctgacg cgcttcgctt gtcacccaga ctggaccgct ggcaacaagt 1920 tgccaccagc ccagaccggg cttatcttaa ttcccatcaa gcaaaggcga aaggaaatcc 1980 tagaacggtt gagaactgga tttttcggtg cccgtttttt gagccaaatt gacaaaatgt 2040 caattttttc gtatttttga gctgtgagtg gtggaaggat gatccccacg ttaaaaaaag 2100 aaatctccaa accgaaagct gtggtggaag gacgatcccc acgttaaaaa aagaaatctc 2160 caaaccgaaa gctgtctgct tacataaaca gtaatacaag gggtgttatg agccatattc 2220 aacgggaaac gtcttgctcg aagccgcgat taaattccaa catggatgct gatttatatg 2280 ggtataaatg ggctcgcgat aatgtcgggc aatcaggtgc gacaatctat cgattgtatg 2340 ggaagcccga tgcgccagag ttgtttctga aacatggcaa aggtagcgtt gccaatgatg 2400 ttacagatga gatggtcaga ctaaactggc tgacggaatt tatgcctctt ccgaccatca 2460 agcattttat ccgtactcct gatgatgcat ggttactcac cactgcgatc cccgggaaaa 2520 cagcattcca ggtattagaa gaatatcctg attcaggtga aaatattgtt gatgcgttgg 2580 cagtgttcct gcgccggttg cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc 2640 gcgtatttcg tctcgctcag gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg 2700 attttgatga cgagcgtaat ggctggcctg ttgaacaagt ctggaaagaa atgcataagc 2760 ttttgccatt ctcaccggat tcagtcgtca ctcatggtga tttctcactt gataacctta 2820 tttttgacga ggggaaatta ataggttgta ttgatgttgg acgagtcgga atcgcagacc 2880 gataccagga tcttgccatc ctatggaact gcctcggtga cttttctcct tcattacaga 2940 aacggctttt tcaaaaatat ggtattgata atcctgatat gaataaattg cagtttcatt 3000 tgatgctcga tgagtttttc taatcagaat tggttaattg gttgtaacac tggcagagca 3060 ttacgctgac ttgacgggac ggcggctttg ttgaataaat cgcattcgcc attcaggctg 3120 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 3180 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 3240 tgtaaaacga cggccagtgc caagcttgca tgcctgcagg tcgactctag aggatccccg 3300 ggtaccgagc tcgaattcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 3360 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 3420 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3480 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3540 tggcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt 3600 tccgtggcaa agcaaaagtt caaaatcagt aaccgtcagt gccgataagt tcaaagttaa 3660 acctggtgtt gataccaaca ttgaaacgct gatcgaaaac gcgctgaaaa acgctgctga 3720 atgtgcgagc ttcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 3780 ggcg 3784 // ID AB033487; SV 1; circular; other DNA; STD; SYN; 3230 BP. XX AC AB033487; XX DT 03-JUL-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct pHA1 DNA, containing cDNA insert for Seriola DE quinqueradiata growth hormone, complete sequence. XX KW Seriola quinqueradiata growth hormone. XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-3230 RA Abe S., Azama K., Tsujimura Y.; RT ; RL Submitted (11-OCT-1999) to the INSDC. RL Shunnosuke Abe, Ehime University College of Agriculture, Department of RL Biological Resouces; 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan RL (E-mail:abe@mcb.agr.ehime-u.ac.jp, Tel:81-89-946-4853, Fax:81-89-946-9853) XX RN [2] RA Abe S., Azama K., Tsujimura Y.; RT "A synthetic circular DNA, constructed of pUC18 Vector and cDNA coded RT Seriola quinqueradiata mature growth hormone"; RL Unpublished. XX DR MD5; c2c6ccb13e68323b94adb02b42aa3389. XX CC A synthetic circular DNA, constructed from pUC18 vector and cDNA CC coded Seriora quinqueradiata mature growth hormone, for gene CC expression in E.coli. This cDNA is inserted between EcoR1 and Xba1 CC site in MCS of pUC18. An expressed protein is a mature growth CC hormone which is added 6 amino-acid (Thr,Met,Ile,Thr,Asn,Ser) at CC its N-terminal side. XX FH Key Location/Qualifiers FH FT source 1..3230 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /clone="pHA1" FT /note="constructed from pUC18 vector and cDNA coded Seriola FT quinqueradiata mature growth hormone" FT /db_xref="taxon:32630" FT CDS 216..800 FT /codon_start=1 FT /transl_table=11 FT /gene="HA1" FT /product="Seriola quinqueradiata growth hormone" FT /protein_id="BAB61718.1" FT /translation="MTMITNSQPITDSQHLFSIAVSRIQNLHLLAQRLFSNFESTLQTE FT DQRQLNKIFLQDFCNSDYIISPIDKHETQRSSVLKLLSISYRLVESWEFSSRFLSGGSA FT LRNQISPRLSELKTGIQLLITANQDGAEMFSDVSALQLAPYGNFYQSLGGEELLRRNYE FT LLACFKKDMHKVETYLTVAKCRLSPEANCTL" XX SQ Sequence 3230 BP; 802 A; 833 C; 797 G; 798 T; 0 other; gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 60 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 120 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 180 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg aattctcagc 240 caatcacaga cagccagcat ctgttctcca tcgctgtcag cagaatccaa aacctccacc 300 tgctcgctca gagactcttc tccaacttcg agagtactct gcagacggag gaccagcgtc 360 aactcaacaa aatcttccta caggatttct gtaactctga ttacatcatc agtcccattg 420 acaagcatga gacacaacgc agctctgttc tgaagctgtt atcgatctcc tatcgattgg 480 tggagtcttg ggagttctcc agtcgctttc tgtctggagg ttctgctctg aggaaccaga 540 tttcacccag actgtctgaa ctcaagacag gaatccaact gctgatcaca gccaatcagg 600 acggagcaga gatgttctct gacgtctcgg ccctccagct cgctccatat ggaaacttct 660 atcagagtct gggaggcgaa gagttgctga gacgaaacta cgaattgctg gcctgtttca 720 agaaggacat gcacaaggtg gagacgtacc tgacggtggc taaatgtcgg ctctctccag 780 aagctaactg caccctgtaa tctagagtcg acctgcaggc atgcaagctt ggcactggcc 840 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca 900 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc 960 caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc ggtattttct ccttacgcat 1020 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca 1080 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg 1140 ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg 1200 ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta 1260 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat 1320 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg 1380 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa 1440 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac 1500 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac 1560 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt 1620 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc 1680 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca 1740 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc 1800 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag 1860 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa 1920 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg 1980 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa 2040 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg 2100 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt 2160 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt 2220 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag 2280 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat 2340 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 2400 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 2460 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 2520 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 2580 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc 2640 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 2700 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 2760 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 2820 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 2880 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 2940 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 3000 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 3060 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 3120 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc 3180 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 3230 // ID AB033750; SV 1; linear; other DNA; STD; SYN; 24 BP. XX AC AB033750; XX DT 26-OCT-1999 (Rel. 61, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, complementary sequence for rat angiotensin type 1 DE receptor mRNA. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-24 RA Takeda H.; RT ; RL Submitted (16-OCT-1999) to the INSDC. RL Hikaru Takeda, Yamagata University, School of Medicine; 2-2-2 Iida-Nishi, RL Yamagata, Yamagata 990-9585, Japan RL (E-mail:hitakeda@med.id.yamagata-u.ac.jp, Tel:81-236-28-5361(ex.5361), RL Fax:81-236-28-5364) XX RN [2] RA Takeda H.; RT "Angiotensin converting enzyme"; RL Unpublished. XX RN [3] RX DOI; 10.1172/JCI116232. RX PUBMED; 8432862. RA Tufro-McReddie A.; RT "Ontogeny of type 1 angiotensin II receptor gene expression in the rat"; RL J. Clin. Invest. 91(2):530-537(1993). XX DR MD5; 147018f8c2d1c1a74f1f03cfc365ee37. XX FH Key Location/Qualifiers FH FT source 1..24 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..24 FT /note="complementary sequence for angiotensin type 1 FT receptor mRNA" XX SQ Sequence 24 BP; 9 A; 4 C; 8 G; 3 T; 0 other; cagaaagccg tagaacagag ggtt 24 // ID AB033751; SV 1; linear; other DNA; STD; SYN; 45 BP. XX AC AB033751; XX DT 26-OCT-1999 (Rel. 61, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, complementary sequence for mouse kidney DE angiotensin converting enzyme mRNA. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-45 RA Takeda H.; RT ; RL Submitted (16-OCT-1999) to the INSDC. RL Hikaru Takeda, Yamagata University, School of Medicine; 2-2-2 Iida-Nishi, RL Yamagata, Yamagata 990-9585, Japan RL (E-mail:hitakeda@med.id.yamagata-u.ac.jp, Tel:81-236-28-5361(ex.5361), RL Fax:81-236-28-5364) XX RN [2] RA Takeda H.; RT "Angiotensin converting enzyme"; RL Unpublished. XX RN [3] RX DOI; 10.1172/JCI116232. RX PUBMED; 8432862. RA Tufro-McReddie A.; RT "Ontogeny of type 1 angiotensin II receptor gene expression in the rat"; RL J. Clin. Invest. 91(2):530-537(1993). XX RN [4] RX DOI; 10.1016/0169-328X(91)90026-T. RX PUBMED; 1662749. RA Whiting P.; RT "Expression of angiotensin converting enzyme mRNA in rat brain"; RL Brain Res. Mol. Brain Res. 11(1):93-96(1991). XX DR MD5; 679c17f77b76dac4ff918fd660a06460. XX FH Key Location/Qualifiers FH FT source 1..45 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..45 FT /note="complementary sequence for mouse kidney angiotensin FT converting enzyme cDNA sequence" XX SQ Sequence 45 BP; 6 A; 10 C; 14 G; 15 T; 0 other; ttctgctcat gttgcttagc agagagttgt actgctgccg ctggg 45 // ID AB036061; SV 1; linear; other DNA; STD; SYN; 258 BP. XX AC AB036061; XX DT 23-DEC-1999 (Rel. 62, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 3) XX DE Synthetic construct rpoA gene for C-terminal domain of the RNA polymerase DE alpha subunit, complete cds. XX KW C-terminal domain of the RNA polymerase alpha subunit. XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-258 RA Wada T., Yamazaki T., Kyogoku Y.; RT ; RL Submitted (21-DEC-1999) to the INSDC. RL Takashi Wada, RIKEN Harima Institute, Structurome Project; 1-1-1, Kouto, RL Mikazuki-cho, Hyogo 679-5148, Japan (E-mail:wadada@spring8.or.jp, RL Tel:81-791-58-2838, Fax:81-791-58-2835) XX RN [2] RX DOI; 10.1074/jbc.275.21.16057. RX PUBMED; 10821859. RA Wada T., Yamazaki T., Kyogoku Y.; RT "The structure and the characteristic DNA binding property of the RT C-terminal domain of the RNA polymerase alpha subunit from Thermus RT thermophilus"; RL J Biol Chem 275(21):16057-16063(2000). XX DR MD5; a50bd78cc903c06b4ac6a87a388e72ca. XX FH Key Location/Qualifiers FH FT source 1..258 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..258 FT /codon_start=1 FT /transl_table=11 FT /gene="rpoA" FT /product="C-terminal domain of the RNA polymerase alpha FT subunit" FT /note="Amino acid sequence except for first Met is FT identical to C-terminal domain of the RNA polymerase alpha FT subunit derived from Thermus thermophilus." FT /note="codon preference based on the codon usage for E. FT coli." FT /protein_id="BAA88567.1" FT /translation="MAVAAPEEAKEPEAPPEQEEELDLPLEELGLSTRVLHSLKEEGIE FT SVRALLALNLKDLKNIPGIGERSLEEIKEALEKKGFTLKE" XX SQ Sequence 258 BP; 78 A; 62 C; 74 G; 44 T; 0 other; atggctgttg ctgcaccgga agaagcgaaa gaaccggaag ccccgccaga gcaggaagaa 60 gaactggacc tgccgctgga agaactgggt ctgtccaccc gtgttctaca ctccctgaaa 120 gaagaaggta tcgaatctgt acgtgctctg ctggcactga acctgaaaga tctgaaaaac 180 atcccgggta tcggcgaacg cagcctggaa gaaattaaag aggcgctcga gaaaaagggc 240 ttcactctta aggagtag 258 // ID AB036341; SV 1; linear; other DNA; STD; SYN; 897 BP. XX AC AB036341; XX DT 26-DEC-1999 (Rel. 62, Created) DT 14-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Synthetic construct gene for CN 8 single chain antibody, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Mus musculus (house mouse) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. XX RN [1] RP 1-897 RA Shinohara N., Demura T., Fukuda H.; RT ; RL Submitted (22-DEC-1999) to the INSDC. RL Naoki Shinohara, Graduate School of Science, University of Tokyo, RL Department of Biological Sciences; Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033, RL Japan (E-mail:shino@biol.s.u-tokyo.ac.jp, Tel:+81-3-5841-4463, RL Fax:+81-3-5841-4462) XX RN [2] RX DOI; 10.1073/pnas.050582197. RX PUBMED; 10706631. RA Shinohara N., Demura T., Fukuda H.; RT "Isolation of a vascular cell wall-specific monoclonal antibody recognizing RT a cell polarity by using a phage display subtraction method"; RL Proc. Natl. Acad. Sci. U.S.A. 97(6):2585-2590(2000). XX DR MD5; ec10c103a9f5a7d5414e53d245d8164d. DR EuropePMC; PMC15972; 10706631. DR IMGT/LIGM; AB036341. XX FH Key Location/Qualifiers FH FT source 1..897 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /note="screened from phage-antibody library constructed FT from immunized mouse spleen" FT /db_xref="taxon:32630" FT source 1..117 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="vector: pCANAB5E" FT /db_xref="taxon:32630" FT source 118..447 FT /organism="Mus musculus" FT /strain="Balb/c" FT /mol_type="other DNA" FT /sex="female" FT /tissue_type="spleen" FT /note="variable region of heavy chain" FT /db_xref="taxon:10090" FT source 448..540 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="flexible linker for single chain antibody" FT /db_xref="taxon:32630" FT source 541..840 FT /organism="Mus musculus" FT /strain="Balb/c" FT /mol_type="other DNA" FT /sex="female" FT /tissue_type="spleen" FT /note="variable region of light chain" FT /db_xref="taxon:10090" FT source 841..897 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="vector: pCANAB5E" FT /db_xref="taxon:32630" FT CDS 1..897 FT /codon_start=1 FT /transl_table=11 FT /gene="CN 8 scFv" FT /product="CN 8 single chain antibody" FT /protein_id="BAA88633.1" FT /translation="MTMITPSFGAFFLEIFNVKKLLFAIPLVVPFYAAQPAMAQVKLQQ FT SGGGLVKPGGSLKLSCAASGSDFSRYWMSWVRQAPGKGLEWIGEINPDSSTINYTPSLK FT DKFIISRDNAKNTLYLQMSKVRSEDTALYYCARASYYGHSAYWGQGTTVTVSSGGGGSG FT GGGSGGGGSDIELTQSPASLSASVGETVTITCRASGNIHNYLAWYQQKQGKSPQLLVYN FT AKTLADGVPSRFSGSGSGTQYSLKINSLQPEDFGSYYCQHFWTTPYTFGGGTKLEIKRA FT AAGAPVPYPDPLEPREA" XX SQ Sequence 897 BP; 234 A; 213 C; 235 G; 215 T; 0 other; atgaccatga ttacgccaag ctttggagcc ttttttttgg agattttcaa cgtgaaaaaa 60 ttattattcg caattccttt agttgttcct ttctatgcgg cccagccggc catggcccag 120 gtgaaactgc agcagtcagg gggaggctta gtgaagcctg gagggtccct gaaactctcc 180 tgtgcagcct caggatccga ttttagtaga tactggatga gttgggtccg gcaggctcca 240 gggaaagggc tagaatggat tggagaaatt aatccagata gcagtacgat aaactatacg 300 ccatctctaa aggataaatt catcatctcc agagacaacg ccaaaaatac gctgtatctg 360 caaatgagca aagtgagatc tgaggacaca gccctttatt actgtgcaag agcctcctac 420 tatggtcact ctgcttactg gggccaaggc accacggtca ccgtctcctc aggtggaggc 480 ggttcaggcg gaggtggctc tggcggtggc ggatcggaca tcgagctcac tcagtctcca 540 gcctccctat ctgcatctgt gggagaaact gtcaccatca catgtcgagc aagtgggaat 600 attcacaatt atttagcatg gtatcagcag aaacagggaa aatctcctca gctcctggtc 660 tataatgcaa aaaccttagc agatggtgtg ccatcaaggt tcagtggcag tggatcagga 720 acacaatatt ctctcaagat caatagcctg cagcctgaag attttgggag ttattactgt 780 caacattttt ggactactcc gtacacgttc ggagggggca ccaagctgga aatcaaacgg 840 gcggccgcag gtgcgccggt gccgtatccg gatccgctgg aaccgcgtga agcatag 897 // ID AB037684; SV 1; circular; other DNA; STD; SYN; 5917 BP. XX AC AB037684; XX DT 04-APR-2000 (Rel. 63, Created) DT 27-DEC-2006 (Rel. 90, Last updated, Version 4) XX DE Synthetic construct cloning vector pPLuc, complete sequence. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-5917 RA Chen A.B., Kao A.Y.-F., Brown C.M.; RT ; RL Submitted (27-JAN-2000) to the INSDC. RL Chris M. Brown, University of Otago, Department of Biochemistry; P.O.Box RL 56, Dunedin, Otago 9001, New Zealand RL (E-mail:chris.brown@stonebow.otago.ac.nz, Tel:+64-3-479-7875, RL Fax:+64-3-479-7866) XX RN [2] RP 1-5917 RX DOI; 10.1093/nar/gki251. RX PUBMED; 15731337. RA Chen A.B., Kao A.Y.-F., Brown C.M.; RT "Translation of the first upstream ORF in the hepatitis B virus pregenomic RT RNA modulates translation at the core and polymerase initiation codons"; RL Nucleic Acids Res. 33(4):1169-1181(2005). XX DR MD5; b34330efc84a489a583a9cd1bfb96a64. DR EuropePMC; PMC3346311; 22418844. DR EuropePMC; PMC549565; 15731337. XX FH Key Location/Qualifiers FH FT source 1..5917 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="cloning vector pPLuc" FT /db_xref="taxon:32630" FT misc_feature 1..495 FT /note="HBV Adw 5' leader of pregenomic RNA" FT CDS 35..94 FT /codon_start=1 FT /transl_table=11 FT /gene="C0" FT /note="unnamed protein product; ORF" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="BAA93573.1" FT /translation="MSHCSSLQAVPWVALGHGH" FT CDS 84..581 FT /codon_start=1 FT /transl_table=11 FT /product="modified C" FT /protein_id="BAA93574.1" FT /translation="MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTASALYREALESP FT EHCSPHHTALRQAILCWGELMTLATWVGNNLEDPATRDLVVNYVNTNMGLKIRQLLWFH FT ISCLTFGRETVLEYLVSFGVWIRTPPAYRPPNARRRRRQKHKERPGAILSAGRWNRWRA FT TA" FT CDS 490..2151 FT /codon_start=1 FT /transl_table=11 FT /gene="PLuc" FT /product="luciferase" FT /note="P codon luciferase fusion" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="BAA93575.1" FT /translation="MPDVEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAF FT TDAHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVA FT VAPANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG FT FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRF FT SHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQ FT DYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIR FT QGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPM FT IMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELE FT SILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRG FT GVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" FT regulatory 5899..5917 FT /function="T7 promoter" FT /note="derived from pGLControl (Promega)" FT /regulatory_class="promoter" XX SQ Sequence 5917 BP; 1523 A; 1420 C; 1409 G; 1565 T; 0 other; aactttttca cctctgccta atcatctctt gtacatgtcc cactgttcaa gcctccaagc 60 tgtgccttgg gtggctttgg ggcatggaca ttgaccctta taaagaattt ggagctactg 120 tggagttact ctcgtttttg ccttctgact tctttccttc cgtcagagat ctcctagaca 180 ccgcctcagc tctgtatcgg gaagccttag agtctcctga gcattgctca cctcaccaca 240 ccgcactcag gcaagccatt ctctgctggg gggaattgat gactctagct acctgggtgg 300 gtaataattt ggaagatcca gcaactaggg atctagtagt caattatgtt aatactaaca 360 tgggtttaaa aattaggcaa ctattgtggt ttcatatatc ttgccttact tttggaagag 420 agactgtact tgaatatttg gtatctttcg gagtgtggat tcgcactcct ccagcctata 480 gaccaccaaa tgcccgacgt cgaagacgcc aaaaacataa agaaaggccc ggcgccattc 540 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc 600 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct 660 gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca 720 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc 780 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg 840 ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa 900 aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct 960 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc 1020 ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg 1080 atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact 1140 gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat 1200 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga 1260 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg 1320 aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc 1380 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct 1440 ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca 1500 ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag 1560 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg 1620 gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt 1680 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag 1740 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc 1800 gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg 1860 gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac 1920 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg 1980 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga 2040 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa 2100 atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagaaa 2160 ctttttcacc tctgcctaat catctcttgt acatgtccca ctgttcaagc ctccaagctg 2220 tgccttgggt ggctttgggg catggacatt gacccttaaa agaatttgga gctacaaaaa 2280 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaagaa ttctagagtc ggggcggccg 2340 gccgcttcga gcagacatga taagatacat tgatgagttt ggacaaacca caactagaat 2400 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat 2460 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca 2520 gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtaaaatcga 2580 taaggatctg aacgatggag cggagaatgg gcggaactgg gcggagttag gggcgggatg 2640 ggcggagtta ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt 2700 ctgcctgctg gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg 2760 ctttgcatac ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca 2820 ttccacagcg gatccgtcga ccgatgccct tgagagcctt caacccagtc agctccttcc 2880 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac 2940 tcgtaggaca ggtgccggca gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 3000 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 3060 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 3120 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 3180 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 3240 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 3300 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat 3360 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 3420 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 3480 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 3540 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 3600 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 3660 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 3720 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 3780 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 3840 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 3900 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 3960 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 4020 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 4080 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 4140 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 4200 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 4260 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 4320 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 4380 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 4440 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 4500 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 4560 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 4620 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 4680 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 4740 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 4800 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 4860 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta 4920 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg 4980 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa 5040 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc 5100 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt 5160 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca 5220 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc 5280 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta 5340 acgtttacaa tttcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg 5400 cgggcctctt cgctattacg ccagcccaag ctaccatgat aagtaagtaa tattaaggta 5460 cgggaggtac ttggagcggc cgcaataaaa tatctttatt ttcattacat ctgtgtgttg 5520 gttttttgtg tgaatcgata gtactaacat acgctctcca tcaaaacaaa acgaaacaaa 5580 acaaactagc aaaataggct gtccccagtg caagtgcagg tgccagaaca tttctctatc 5640 gataggtacc gagctcttac gcgtgctagc ccgggctcga gatcgatctg cgatctgcat 5700 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg 5760 cccagttccg cccattctcc gccccatcgc tgactaattt tttttattta tgcagaggcc 5820 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta 5880 ggcttttgca aaaagcttta atacgactca ctatagg 5917 // ID AB038146; SV 1; circular; other DNA; STD; SYN; 3249 BP. XX AC AB038146; XX DT 04-APR-2000 (Rel. 63, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 5) XX DE Cloning vector pGFPTA DNA, complete sequence. XX KW . XX OS Cloning vector pGFPTA OC other sequences; artificial sequences; vectors. XX OS Cloning vector pGFPuv OC other sequences; artificial sequences; vectors. XX OS Cloning vector pBR322 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-3249 RA Ito Y., Suzuki M., Husimi Y.; RT ; RL Submitted (07-FEB-2000) to the INSDC. RL Yuzuru Husimi, Saitama University, Deptartment of Functional Materials RL Science; 255 Shimo-ookubo, Urawa, Saitama 338-8750, Japan RL (E-mail:ito@evolve.fms.saitama-u.ac.jp, Tel:+81-48-858-3531, RL Fax:+81-48-858-3531) XX RN [2] RX DOI; 10.1016/S0378-1119(00)00039-1. RX PUBMED; 10713445. RA Ito Y., Suzuki M., Husimi Y.; RT "A T-extended vector using a green fluorescent protein as an indicator"; RL Gene 245(1):59-63(2000). XX DR MD5; 4866426c30f46763cfdd1f8c240dcb02. XX FH Key Location/Qualifiers FH FT source 1..3249 FT /organism="Cloning vector pGFPTA" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:116587" FT source 1..956 FT /organism="Cloning vector pGFPuv" FT /mol_type="other DNA" FT /db_xref="taxon:49914" FT source 957..3249 FT /organism="Cloning vector pBR322" FT /mol_type="other DNA" FT /db_xref="taxon:47470" FT CDS 162..950 FT /codon_start=1 FT /transl_table=11 FT /gene="gfpuv4" FT /product="GFPuv4" FT /protein_id="BAA93576.1" FT /translation="MTMITPSLHACRSTLEDPRVPVEKMSKGEELFTGVVPILVELDGD FT VNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKR FT HDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGH FT KLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN FT HYLSTQSALLKDPNEKRDHMVLLEFVTAAGITHGMDELYK" FT misc_feature 952..957 FT /note="EcoRI/PvuII restriction site" FT CDS 1163..2023 FT /codon_start=1 FT /transl_table=11 FT /gene="bla" FT /product="beta-lactamase" FT /protein_id="BAA93577.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" XX SQ Sequence 3249 BP; 858 A; 774 C; 789 G; 828 T; 0 other; ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 60 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 120 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 180 gcttgcatgc ctgcaggtcg actctagagg atccccgggt accggtagaa aaaatgagta 240 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta 300 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta 360 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta 420 ctctgacgta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa cggcatgact 480 ttttcaagag tgccatgccc gaaggttatg tacaggaacg cacgatatct ttcaaagatg 540 acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt gttaatcgta 600 tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac aaactcgagt 660 acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat ggaatcaaag 720 ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca gaccattatc 780 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcga 840 cacaatctgc ccttttgaaa gatcccaacg aaaagcgtga ccacatggtc cttcttgagt 900 ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaataa tgaattcttg 960 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 1020 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 1080 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 1140 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 1200 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 1260 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 1320 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 1380 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat 1440 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 1500 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 1560 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 1620 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1680 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac 1740 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1800 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1860 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1920 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1980 acagatcgct gagataggtg cgtcactgat taagcattgg taactgtcag accaagttta 2040 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 2100 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 2160 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 2220 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga 2280 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt 2340 ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata 2400 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac 2460 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg 2520 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg 2580 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag 2640 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct 2700 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc 2760 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt 2820 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg 2880 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga 2940 gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg 3000 cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 3060 aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg 3120 ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa 3180 gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc 3240 gcgaggcag 3249 // ID AB038599; SV 1; circular; other DNA; STD; SYN; 3877 BP. XX AC AB038599; XX DT 22-FEB-2001 (Rel. 66, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 3) XX DE Cloning vector pPV2 DNA, complete sequence. XX KW . XX OS Cloning vector pPV2 OC other sequences; artificial sequences; vectors. XX OS Human parvovirus B19 OC Viruses; Parvoviridae; Parvovirinae; Erythroparvovirus. XX RN [1] RP 1-3877 RA Hashinaka K.; RT ; RL Submitted (21-FEB-2000) to the INSDC. RL Kazuya Hashinaka, Miyazaki Medical College, Department of Biochemistry; RL 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan RL (E-mail:hasinaka@post1.miyazaki-med.ac.jp, Tel:81-985-85-0985, RL Fax:81-985-85-2401) XX RN [2] RA Hashinaka K.; RT "Synthetic Autonomous Vectors Based on Palindromic Sequences of Parvovirus RT B19"; RL Unpublished. XX DR MD5; 453a335854ed88b08ff283dd0e060cd4. XX FH Key Location/Qualifiers FH FT source 1..3877 FT /organism="Cloning vector pPV2" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:117917" FT source 6..479 FT /organism="Human parvovirus B19" FT /mol_type="other DNA" FT /note="synonym:Parvovirus B19" FT /db_xref="taxon:10798" FT source 494..977 FT /organism="Human parvovirus B19" FT /mol_type="other DNA" FT /note="synonym:Parvovirus B19" FT /db_xref="taxon:10798" FT repeat_region 9..391 FT repeat_region 586..968 FT CDS 1809..2669 FT /codon_start=1 FT /transl_table=11 FT /gene="ampR" FT /product="beta-lactamase" FT /protein_id="BAB32736.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" XX SQ Sequence 3877 BP; 931 A; 981 C; 973 G; 992 T; 0 other; atgcatggcc aaatcagatg ccgccggtcg ccgccggtag gcgggacttc cggtacaaga 60 tggcggacaa ttacgtcatt tcctgtgacg tcatttcctg tgacgtcact tccggtgggc 120 gggacttccg gaattagggt tggctctggg ccagcgcttg gggttgcctt gacactaaga 180 caagcggcgc gccgcttgat cttagtggca cgtcaacccc aagcaagctg gcccagagcc 240 aaccctaatt ccggaagtcc cgcccaccgg aagtgacgtc acaggaaatg acgtcacagg 300 aaatgacgta attgtccgcc atcttgtacc ggaagtcccg cctaccggcg gcgaccggcg 360 gcatctgatt tggtgtcttc ttttaaattt tagcgggctt ttttcccgcc ttatgcaaat 420 gggcagccat tttaagtgtt ttactataat tttattggtt agttttgtaa cgaaggtacc 480 gaattcctcg agtctagtgg gcagatctta agattgtgta atatgtatca aaatttagaa 540 aaataaacat ttattgtggt taaaaaatta tgttgttgcg ctttaaaaat ttaaaagaag 600 acaccaaatc agatgccgcc ggtcgccgcc ggtaggcggg acttccggta caagatggcg 660 gacaattacg tcatttcctg tgacgtcatt tcctgtgacg tcacttccgg tgggcgggac 720 ttccggaatt agggttggct ctgggccagc ttgcttgggg ttgacgtgcc actaagatca 780 agcggcgcgc cgcttgtctt agtgtcaagg caaccccaag cgctggccca gagccaaccc 840 taattccgga agtcccgccc accggaagtg acgtcacagg aaatgacgtc acaggaaatg 900 acgtaattgt ccgccatctt gtaccggaag tcccgcctac cggcggcgac cggcggcatc 960 tgatttggcc acgggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc 1020 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca 1080 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa 1140 cagttgcgca gcctgaatgg cgaatggacg cgccctgtag cggcgcatta agcgcggcgg 1200 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 1260 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 1320 gggggctccc tttagggttc cgatttagag ctttacggca cctcgaccgc aaaaaacttg 1380 atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 1440 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 1500 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 1560 aaaatgagct gatttaacaa atatttaacg cgaattttaa caaaatatta acgtttacaa 1620 tttcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacag 1680 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 1740 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 1800 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 1860 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 1920 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 1980 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtcatacac 2040 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgggcgcgg tattctcaga 2100 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 2160 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 2220 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 2280 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 2340 ccacgatgcc tgtagcaatg ccaacaacgt tgcgcaaact attaactggc gaactactta 2400 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 2460 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 2520 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 2580 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 2640 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 2700 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 2760 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 2820 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 2880 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 2940 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 3000 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 3060 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 3120 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 3180 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 3240 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 3300 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 3360 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 3420 tatcgaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 3480 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 3540 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 3600 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 3660 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg 3720 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt 3780 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg 3840 ccaagctatt taggtgacac tatagaatac tcaagct 3877 // ID AB038600; SV 1; circular; other DNA; STD; SYN; 6320 BP. XX AC AB038600; XX DT 22-FEB-2001 (Rel. 66, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 3) XX DE Cloning vector pPVLUC441 DNA, complete sequence. XX KW . XX OS Cloning vector pPVLUC441 OC other sequences; artificial sequences; vectors. XX OS Human parvovirus B19 OC Viruses; Parvoviridae; Parvovirinae; Erythroparvovirus. XX RN [1] RP 1-6320 RA Hashinaka K.; RT ; RL Submitted (21-FEB-2000) to the INSDC. RL Kazuya Hashinaka, Miyazaki Medical College, Department of Biochemistry; RL 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan RL (E-mail:hasinaka@post1.miyazaki-med.ac.jp, Tel:81-985-85-0985, RL Fax:81-985-85-2401) XX RN [2] RA Hashinaka K.; RT "Synthetic Autonomous Vectors Based on Palindromic Sequences of Parvovirus RT B19"; RL Unpublished. XX DR MD5; 05f6dcc64428a1a40be257e950f9ead5. XX FH Key Location/Qualifiers FH FT source 1..6320 FT /organism="Cloning vector pPVLUC441" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:117918" FT source 6..479 FT /organism="Human parvovirus B19" FT /mol_type="other DNA" FT /note="synonym:Parvovirus B19" FT /db_xref="taxon:10798" FT source 2937..3420 FT /organism="Human parvovirus B19" FT /mol_type="other DNA" FT /note="synonym:Parvovirus B19" FT /db_xref="taxon:10798" FT repeat_region 9..391 FT CDS 1347..2414 FT /codon_start=1 FT /transl_table=11 FT /gene="luc" FT /product="luciferase" FT /protein_id="BAB32737.1" FT /translation="MNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSV FT VPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKST FT LIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDK FT PGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGW FT LHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDD FT AGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKI FT REILIKAKKGGKIAV" FT repeat_region 3029..3411 FT CDS 4252..5112 FT /codon_start=1 FT /transl_table=11 FT /gene="ampR" FT /product="beta-lactamase" FT /protein_id="BAB32738.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCHTLLSRIDAGQEQLGRRARYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" XX SQ Sequence 6320 BP; 1580 A; 1527 C; 1589 G; 1624 T; 0 other; atgcatggcc aaatcagatg ccgccggtcg ccgccggtag gcgggacttc cggtacaaga 60 tggcggacaa ttacgtcatt tcctgtgacg tcatttcctg tgacgtcact tccggtgggc 120 gggacttccg gaattagggt tggctctggg ccagcgcttg gggttgcctt gacactaaga 180 caagcggcgc gccgcttgat cttagtggca cgtcaacccc aagcaagctg gcccagagcc 240 aaccctaatt ccggaagtcc cgcccaccgg aagtgacgtc acaggaaatg acgtcacagg 300 aaatgacgta attgtccgcc atcttgtacc ggaagtcccg cctaccggcg gcgaccggcg 360 gcatctgatt tggtgtcttc ttttaaattt tagcgggctt ttttcccgcc ttatgcaaat 420 gggcagccat tttaagtgtt ttactataat tttattggtt agttttgtaa cgaaggtacc 480 gagctcttac gcgtgctagc ccgggctcga gatctgcgat ctgcatctca attagtcagc 540 aaccatagtc ccgcccctaa ctccgcccat cccgacccct aactccgccc agttccgccc 600 attctccgcc ccatcgctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg 660 cctctgagct attccaagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 720 aagcttggca ttccggtact gttggtaaag ccaccatgga agacgccaaa aacataaaag 780 aaaggcccgg cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag 840 gctatgaaga gatacgccct ggttcctgga acaattgact tttacagatg cacatatcga 900 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa 960 acgatatggg ctgaatacaa aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat 1020 tctttatgcc ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca 1080 atttataatg aacgtgaatt gctcaacagt atgggcattt cgcagcctac cgtggtgttc 1140 gtttccaaaa aggggttgca aaaaattttg aacgtgcaaa aaaaagctcc caatcatcca 1200 aaaaattatt atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt 1260 cgtcacatct catctacctc cacggtttta atgaatacga ttttgtgcca gagtccttcg 1320 atagggacaa gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag 1380 gtgtcgctct gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt 1440 ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt 1500 ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata 1560 gatttgaaga agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc 1620 tggtgccaac cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat 1680 ctaatttaca cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg 1740 ttgccaagag gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat 1800 cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc 1860 cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa 1920 gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag 1980 cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata gcttactggg 2040 acgaagacga acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct 2100 atcaggtggc tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg 2160 caggtgtcgc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt 2220 tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa 2280 caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta 2340 ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa 2400 agatcgccgt gtaattctag agtcggggcg gccggccgct tcgagcagac atgataagat 2460 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg 2520 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca 2580 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa 2640 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tctgaacgat ggagcggaga 2700 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt 2760 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt 2820 tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga 2880 gcctggggac tttccacacc ctaactgaca cacattccac agcggatccg tcgagtctag 2940 tgggcagatc ttaagattgt gtaatatgta tcaaaattta gaaaaataaa catttattgt 3000 ggttaaaaaa ttatgttgtt gcgctttaaa aatttaaaag aagacaccaa atcagatgcc 3060 gccggtcgcc gccggtaggc gggacttccg gtacaagatg gcggacaatt acgtcatttc 3120 ctgtgacgtc atttcctgtg acgtcacttc cggtgggcgg gacttccgga attagggttg 3180 gctctgggcc agcttgcttg gggttgacgt gccactaaga tcaagcggcg cgccgcttgt 3240 cttagtgtca aggcaacccc aagcgctggc ccagagccaa ccctaattcc ggaagtcccg 3300 cccaccggaa gtgacgtcac aggaaatgac gtcacaggaa atgacgtaat tgtccgccat 3360 cttgtaccgg aagtcccgcc taccggcggc gaccggcggc atctgatttg gccacgggcc 3420 caattcgccc tatagtgagt cgtattacaa ttcactggcc gtcgttttac aacgtcgtga 3480 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 3540 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 3600 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc 3660 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc 3720 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg 3780 ttccgattta gagctttacg gcacctcgac cgcaaaaaac ttgatttggg tgatggttca 3840 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc 3900 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct 3960 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa 4020 caaatattta acgcgaattt taacaaaata ttaacgttta caatttcgcc tgatgcggta 4080 ttttctcctt acgcatctgt gcggtatttc acaccgcata caggtggcac ttttcgggga 4140 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 4200 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt 4260 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct 4320 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt 4380 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt 4440 tttccaatga tgagcacttt taaagttctg ctatgtcata cactattatc ccgtattgac 4500 gccgggcaag agcaactcgg tcgccgggcg cggtattctc agaatgactt ggttgagtac 4560 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct 4620 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg 4680 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg 4740 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca 4800 atgccaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa 4860 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt 4920 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc 4980 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg 5040 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt 5100 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt 5160 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc 5220 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct 5280 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta 5340 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc 5400 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac 5460 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct 5520 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat 5580 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg 5640 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa 5700 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg 5760 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga 5820 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatcgaa aaacgccagc 5880 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct 5940 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct 6000 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca 6060 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 6120 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat 6180 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc 6240 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct atttaggtga 6300 cactatagaa tactcaagct 6320 // ID AB038601; SV 1; circular; other DNA; STD; SYN; 2727 BP. XX AC AB038601; XX DT 22-FEB-2001 (Rel. 66, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 3) XX DE Cloning vector pLCPV101 DNA, complete sequence. XX KW . XX OS Cloning vector pLCPV101 OC other sequences; artificial sequences; vectors. XX OS Human parvovirus B19 OC Viruses; Parvoviridae; Parvovirinae; Erythroparvovirus. XX RN [1] RP 1-2727 RA Hashinaka K.; RT ; RL Submitted (21-FEB-2000) to the INSDC. RL Kazuya Hashinaka, Miyazaki Medical College, Department of Biochemistry; RL 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan RL (E-mail:hasinaka@post1.miyazaki-med.ac.jp, Tel:81-985-85-0985, RL Fax:81-985-85-2401) XX RN [2] RA Hashinaka K.; RT "Synthetic Autonomous Vectors Based on Palindromic Sequences of Parvovirus RT B19"; RL Unpublished. XX DR MD5; 14d6162c19a01230561bfc5b454e4db5. XX FH Key Location/Qualifiers FH FT source 1..2727 FT /organism="Cloning vector pLCPV101" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:117919" FT source 12..485 FT /organism="Human parvovirus B19" FT /mol_type="other DNA" FT /note="synonym:Parvovirus B19" FT /db_xref="taxon:10798" FT source 500..983 FT /organism="Human parvovirus B19" FT /mol_type="other DNA" FT /note="synonym:Parvovirus B19" FT /db_xref="taxon:10798" FT repeat_region 15..397 FT repeat_region 592..974 FT CDS complement(1971..2630) FT /codon_start=1 FT /transl_table=11 FT /gene="CmR" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BAB32739.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVHLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHTEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" XX SQ Sequence 2727 BP; 683 A; 678 C; 674 G; 692 T; 0 other; ggatccatgc atggccaaat cagatgccgc cggtcgccgc cggtaggcgg gacttccggt 60 acaagatggc ggacaattac gtcatttcct gtgacgtcat ttcctgtgac gtcacttccg 120 gtgggcggga cttccggaat tagggttggc tctgggccag cgcttggggt tgccttgaca 180 ctaagacaag cggcgcgccg cttgatctta gtggcacgtc aaccccaagc aagctggccc 240 agagccaacc ctaattccgg aagtcccgcc caccggaagt gacgtcacag gaaatgacgt 300 cacaggaaat gacgtaattg tccgccatct tgtaccggaa gtcccgccta ccggcggcga 360 ccggcggcat ctgatttggt gtcttctttt aaattttagc gggctttttt cccgccttat 420 gcaaatgggc agccatttta agtgttttac tataatttta ttggttagtt ttgtaacgaa 480 ggtaccgaat tcctcgagtc tagtgggcag atcttaagat tgtgtaatat gtatcaaaat 540 ttagaaaaat aaacatttat tgtggttaaa aaattatgtt gttgcgcttt aaaaatttaa 600 aagaagacac caaatcagat gccgccggtc gccgccggta ggcgggactt ccggtacaag 660 atggcggaca attacgtcat ttcctgtgac gtcatttcct gtgacgtcac ttccggtggg 720 cgggacttcc ggaattaggg ttggctctgg gccagcttgc ttggggttga cgtgccacta 780 agatcaagcg gcgcgccgct tgtcttagtg tcaaggcaac cccaagcgct ggcccagagc 840 caaccctaat tccggaagtc ccgcccaccg gaagtgacgt cacaggaaat gacgtcacag 900 gaaatgacgt aattgtccgc catcttgtac cggaagtccc gcctaccggc ggcgaccggc 960 ggcatctgat ttggccacgg gcccggatcc tcatgaccaa aatcccttaa cgtgagtttt 1020 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 1080 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 1140 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 1200 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 1260 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 1320 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 1380 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 1440 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 1500 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 1560 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 1620 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac 1680 ggttcctggc cttttgctgg ccttttgctc acatgtaatg tgagttagct cactcattag 1740 gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga 1800 taacaatttc acacaggaaa cagctatgac catgattacg ccaagctacg taatacgact 1860 cactagcttg gcactgggcc aagctgaatt tctgccattc atccgcttat tatcacttat 1920 tcaggcgtag caccaggcgt ttaagggcac caataactgc cttaaaaaaa ttacgccccg 1980 ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac atggaagcca 2040 tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta 2100 taatatttgc ccatagtgaa aacgggggcg aagaagttgt ccatattagc cacgtttaaa 2160 tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt ctcaataaac 2220 cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga atatatgtgt 2280 agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt ttcagtttgc 2340 tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc accgtctttc 2400 attgccatac gaaattccgt atgagcattc atcaggcggg caagaatgtg aataaaggcc 2460 ggataaaact tgtgcttatt tttctttacg gtctttaaaa aggccgtaat atccagatga 2520 acggtctggt tataggtaca ttgagcaact gactgaaatg cctcaaaatg ttctttacga 2580 tgccattggg atatatcaac ggtggtatat ccagtgattt ttttctccat tttagcttcc 2640 ttagctcctg aaaatctcga taactcaaaa aatacgcccg gtagtgatct tatttcatta 2700 tggtgaaagt tggaacctct tacatct 2727 // ID AB038602; SV 1; circular; other DNA; STD; SYN; 5590 BP. XX AC AB038602; XX DT 22-FEB-2001 (Rel. 66, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 3) XX DE Cloning vector pLCPVRGNR104 DNA, complete sequence. XX KW . XX OS Cloning vector pLCPVRGNR104 OC other sequences; artificial sequences; vectors. XX OS Human parvovirus B19 OC Viruses; Parvoviridae; Parvovirinae; Erythroparvovirus. XX RN [1] RP 1-5590 RA Hashinaka K.; RT ; RL Submitted (21-FEB-2000) to the INSDC. RL Kazuya Hashinaka, Miyazaki Medical College, Department of Biochemistry; RL 5200 Kihara, Kiyotake, Miyazaki 889-1692, Japan RL (E-mail:hasinaka@post1.miyazaki-med.ac.jp, Tel:81-985-85-0985, RL Fax:81-985-85-2401) XX RN [2] RA Hashinaka K.; RT "Synthetic Autonomous Vectors Based on Palindromic Sequences of Parvovirus RT B19"; RL Unpublished. XX DR MD5; 0fb025712c264942e2c1dfb3fb1d580b. DR EuropePMC; PMC5399791; 27903913. XX FH Key Location/Qualifiers FH FT source 1..5590 FT /organism="Cloning vector pLCPVRGNR104" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:117920" FT source 12..485 FT /organism="Human parvovirus B19" FT /mol_type="other DNA" FT /note="synonym:Parvovirus B19" FT /db_xref="taxon:10798" FT source 3369..3846 FT /organism="Human parvovirus B19" FT /mol_type="other DNA" FT /note="synonym:Parvovirus B19" FT /db_xref="taxon:10798" FT repeat_region 15..397 FT CDS complement(1115..2635) FT /codon_start=1 FT /transl_table=11 FT /gene="rsGFP-neoR" FT /product="red-shift green fluorescent protein-fused FT neomycin phosphotransferase" FT /protein_id="BAB32740.1" FT /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL FT KFICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD FT GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK FT VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL FT EFVTAAGITHGMDELYNGAGAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRL FT SAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVP FT GQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD FT LDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVAD FT RYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT repeat_region 3455..3837 FT CDS complement(4834..5493) FT /codon_start=1 FT /transl_table=11 FT /gene="CmR" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BAB32741.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVHLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHTEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" XX SQ Sequence 5590 BP; 1330 A; 1460 C; 1444 G; 1356 T; 0 other; ggatccatgc atggccaaat cagatgccgc cggtcgccgc cggtaggcgg gacttccggt 60 acaagatggc ggacaattac gtcatttcct gtgacgtcat ttcctgtgac gtcacttccg 120 gtgggcggga cttccggaat tagggttggc tctgggccag cgcttggggt tgccttgaca 180 ctaagacaag cggcgcgccg cttgatctta gtggcacgtc aaccccaagc aagctggccc 240 agagccaacc ctaattccgg aagtcccgcc caccggaagt gacgtcacag gaaatgacgt 300 cacaggaaat gacgtaattg tccgccatct tgtaccggaa gtcccgccta ccggcggcga 360 ccggcggcat ctgatttggt gtcttctttt aaattttagc gggctttttt cccgccttat 420 gcaaatgggc agccatttta agtgttttac tataatttta ttggttagtt ttgtaacgaa 480 ggtaccgaat tcctcgacgg tatacagaca tgataagata cattgatgag tttggacaaa 540 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt 600 tatttgtaac cattataagc tgcaataaac aagttggggt gggcgaagaa ctccagcatg 660 agatccccgc gctggaggat catccagccg gcgaacgtgg cgagaaagga agggaagaaa 720 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 780 acacccgccg cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag 840 ctggttcttt ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg 900 tcttcccaat cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac 960 ctactcagac aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca 1020 ccttccaggg tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa 1080 ggcacagtcg aggctgatca gcgagctcga attctcagaa gaactcgtca agaaggcgat 1140 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag 1200 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc 1260 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca 1320 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc 1380 gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat 1440 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg 1500 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag 1560 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca 1620 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc 1680 aaggaacgcc cgtcgtggcg agccacgata gccgcgctgc ctcgtcttgc agttcattca 1740 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga 1800 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct 1860 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcgatagca ccagcaccgt 1920 tgtacagttc atccatgcca tgtgtaatcc cagcagctgt tacaaactca agaaggacca 1980 tgtggtctct cttttcgttg ggatctttcg aaagggcaga ttgtgtggac aggtaatggt 2040 tgtctggtaa aaggacaggg ccatcgccaa ttggagtatt ttgttgataa tggtctgcta 2100 gttgaacgct tccatcttca atgttgtggc gggtcttgaa gttcactttg attccattct 2160 tttgtttgtc tgccatgatg tatacattgt gtgagttata gttgtattcc aatttgtgtc 2220 ccagaatgtt gccatcttcc ttgaagtcaa taccttttaa ctcgattcta ttaacaaggg 2280 tatcaccttc aaacttgact tcagcacgtg tcttgtagtt gccgtcatct ttgaagaaga 2340 tggtcctttc ctgtacataa ccttcgggca tggcactctt gaaaaagtca tgccgtttca 2400 tatgatccgg gtatcttgaa aagcattgaa caccatagca cagagtagtg actagggtgg 2460 gccatggaac aggcagtttg ccagtagtgc agatgaactt cagggtaagt tttccgtatg 2520 ttgcatcacc ttcaccctct ccactgacag agaacttgtg gccgttaaca tcaccatcta 2580 attcaacaag aattgggaca actccagtga agagttcttc tcctttgcta gccatttctt 2640 gcgcgcccga tcctgaacgg cagaggttac ggcagtttgt ctctccccct tccgggagcc 2700 accttcttct ccaaccgtcc cggtcgcgct ctcggcgctt ctgaggagag aactggctga 2760 gtgacgccct ttatagattc gcccttgtgt cccgcccctt cctttcccgc cctcccttgc 2820 gctacggggc cgcccgcacc ggcctacacg gagcgcgcgc ggcggagttg ttgacgctag 2880 ggctccggct ccctggttgg gtgttctttc tgacgcgaca ggaggaggag aatgtcctgg 2940 tcctgtcgtc ctcctttcgg gtttcccgtg cactcaaacc gaggacttac agaacggagg 3000 ataaagttag gccattttta ctcagcttcg gagttcaggc tcatttttca gctaaagtct 3060 ctcattagta tccccccaca cacatcggga aaatggtttg tcctacgcat cggtaatgaa 3120 ggcggggccc ttcgggtcct ccggagcggg ttccgggggt ggggggaagg agggagggac 3180 gggacgggcc tcgttcatga atattcagtt caccgctgaa tatgcataag gcaggcaaga 3240 tggcgcgtcc aatcaattgg aagtagccgt tattagtgga gaggccccag gacgttgggg 3300 caccgcctgt gctctagtag ctttacggag ccctggcgct cgatgttcaa gcccaagctt 3360 tcgcgagctc gagatcttaa gattgtgtaa tatgtatcaa aatttagaaa aataaacatt 3420 tattgtggtt aaaaaattat gttgttgcgc tttaaaaatt taaaagaaga caccaaatca 3480 gatgccgccg gtcgccgccg gtaggcggga cttccggtac aagatggcgg acaattacgt 3540 catttcctgt gacgtcattt cctgtgacgt cacttccggt gggcgggact tccggaatta 3600 gggttggctc tgggccagct tgcttggggt tgacgtgcca ctaagatcaa gcggcgcgcc 3660 gcttgtctta gtgtcaaggc aaccccaagc gctggcccag agccaaccct aattccggaa 3720 gtcccgccca ccggaagtga cgtcacagga aatgacgtca caggaaatga cgtaattgtc 3780 cgccatcttg taccggaagt cccgcctacc ggcggcgacc ggcggcatct gatttggcca 3840 cgggcccgga tcctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 3900 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 3960 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 4020 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 4080 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 4140 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 4200 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 4260 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 4320 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 4380 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 4440 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 4500 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 4560 tggccttttg ctcacatgta atgtgagtta gctcactcat taggcacccc aggctttaca 4620 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 4680 aaacagctat gaccatgatt acgccaagct acgtaatacg actcactagc ttggcactgg 4740 gccaagctga atttctgcca ttcatccgct tattatcact tattcaggcg tagcaccagg 4800 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca 4860 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaga cggcatgatg 4920 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatagt 4980 gaaaacgggg gcgaagaagt tgtccatatt agccacgttt aaatcaaaac tggtgaaact 5040 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc 5100 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc 5160 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta 5220 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc 5280 cgtatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt 5340 atttttcttt acggtcttta aaaaggccgt aatatccaga tgaacggtct ggttataggt 5400 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc 5460 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct 5520 cgataactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc 5580 tcttacatct 5590 // ID AB040452; SV 1; circular; other DNA; STD; SYN; 6140 BP. XX AC AB040452; XX DT 31-MAR-2000 (Rel. 63, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 3) XX DE Shuttle vector pRES19 DNA, complete sequence. XX KW beta-galactosidase; beta-lactamase; rep protein; rRNA methylase. XX OS Shuttle vector pRES19 OC other sequences; artificial sequences; vectors. XX OS Cloning vector pUC19 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-6140 RA Ishikawa J., Sudoh M.; RT ; RL Submitted (22-MAR-2000) to the INSDC. RL Jun Ishikawa, National Institute of Infectious Diseases, Department of RL Bioactive Molecules; 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan RL (E-mail:jun@nih.go.jp, URL:http://www.nih.go.jp/niid/index-e.html, RL Tel:81-3-5285-1111(ex.2354), Fax:81-3-5285-1272) XX RN [2] RA Ishikawa J., Sudoh M., Hotta K.; RT "Nucleotide sequence of the pRES19."; RL Unpublished. XX RN [3] RX DOI; 10.1016/0378-1097(96)00397-7. RX PUBMED; 8931335. RA Ishikawa J., Niino Y., Hotta K.; RT "Construction of pRES18 and pRES19, Streptomyces-Escherichia coli shuttle RT vectors carrying multiple cloning sites"; RL FEMS Microbiol. Lett. 145(1):113-116(1996). XX DR MD5; 8d2ad7727f9a37ad69619c882c63a596. XX FH Key Location/Qualifiers FH FT source 1..6140 FT /organism="Shuttle vector pRES19" FT /focus FT /lab_host="Streptomyces griseus" FT /mol_type="other DNA" FT /note="Streptomyces-Escherichia coli shuttle vector" FT /db_xref="taxon:119636" FT source 2370..5055 FT /organism="Cloning vector pUC19" FT /mol_type="other DNA" FT /db_xref="taxon:31851" FT CDS complement(join(5287..6140,1..175)) FT /codon_start=1 FT /transl_table=11 FT /gene="rep" FT /product="rep protein" FT /note="probable" FT /protein_id="BAA93043.1" FT /translation="MAQGQNGKVDRVKIHVRDGVAYTSGLCICANGWLCPVCSAKIRAK FT RANEIAVGVASLIMGGGEAWMVTFTVRHARGHALADLLNALNDAFRKLGTGVMAVREKK FT ATGKIGTINSREITYGKNGWHPHLHVIVCFKEVPDVRELAIMMGRWQRLWMAWTKKHGF FT PADKAHGVKWDKVMTAEGAGEYIAKAQDSGKHIGNEVARGDLKKGKLGSLTPFEMIEYL FT MATGDAAVVDLWLEYEKATKGKSWLRWSPKLKAMLGVDEKTEEELAAEEVAGGEVAELA FT EAAWKMVIRHGLEARVLEAVERGGFPALVKLLTAHQIWAVKKLTPDDASETPDGPSESN FT AA" FT CDS complement(1216..2025) FT /codon_start=1 FT /transl_table=11 FT /gene="tsr" FT /product="rRNA methylase" FT /protein_id="BAA93040.1" FT /translation="MTELDTIANPSDPAVQRIIDVTKPSRSNIKTTLIEDVEPLMHSIA FT AGVEFIEVYGSDSSPFPSELLDLCGRQNIPVRLIDSSIVNQLFKGERKAKTFGIARVPR FT PARFGDIASRRGDVVVLDGVKIVGNIGAIVRTSLALGASGIILVDSDITSIADRRLQRA FT SRGYVFSLPVVLSGREEAIAFIRDSGMQLMTLKADGDISVKELGDNPDRLALLFGSEKG FT GPSDLFEEASSASVSIPMMSQTESLNVSVSLGIALHERIDRNLAANR" FT CDS 2808..3668 FT /codon_start=1 FT /transl_table=11 FT /gene="bla" FT /product="beta-lactamase" FT /protein_id="BAA93041.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT CDS 4825..5103 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ" FT /product="beta-galactosidase" FT /protein_id="BAA93042.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRVEGPLPVSGGGPFL" FT misc_feature 4842..4898 FT /note="multiple cloning sites" XX SQ Sequence 6140 BP; 1277 A; 1794 C; 1753 G; 1316 T; 0 other; tgatcagcga ggcgacaccc acagcgatct cgttcgccct cttggcgcgg atcttggccg 60 agcagaccgg gcagagccag ccgttcgcac agatgcagag cccggacgtg taggccaccc 120 cgtcacgtac gtggatcttg acccggtcca ccttgccgtt ctggccctga gccatgaccc 180 gtccgcaggc cccgagacgc ttgaccgtcg tgaagctgac gatggcccgc tgggcgtcgt 240 accggcgacg acgtcggcaa cacggatcgg tgcccttccc gcattcgtgc tggtcagacc 300 cggtttcccc cgaggggata gaaacaaagt ccgtagtagt accaagcggg gcgtccgcca 360 ggccgccaga ggcggcccgg cgcccctcaa gggcttcgcc aacggggcgc cgggagacct 420 ggcggccacc atgggcccgc aggtgtttcg cggcctgtgc cccatccagc gggaggaccg 480 ggggcacgtc atcatgcggg gtggctacct gtggtgccgg acccttgccg tgctctaccg 540 tggtcatcga tcaacttctc cgttcgcgtt ccaggtggcg gaggaagagg cccggaccgt 600 cgtcagcggt tccgggcctc cgtcgttcca tgcgatcctg acggccgtgg ccgaccggcg 660 agctatggac tcgccgggcg gtcacttctc cgtctgcgag cccatcagcg tcttgatgac 720 cagccagccg atcgcgcagg agatcgcgcc gatgccgacc gcgacggcga cgacggcgac 780 ggccaggagg acggcgacga ccaggacccc ggcaccgcca gcggccacga tctgtccggc 840 gtgctgacgg gtccaggagg gcgcaggggc cgcctgctgg acgatgacgt agccgggggt 900 ctgctggacg ctctgaggga cgctcagcgc ctgtccgggg ccgatgtgaa tgggtgtcgg 960 ggtgtagacc gtttcggggc cgtaggacgt cgtcacgggg tgcctccgag gtcttccagc 1020 tcgatggacc agaaccgcgt cagcgggacg acgcgcgaga agttccggcc cgtctccttc 1080 tccatgcttt cgacggcgag ttccacagcg cggcggtaga tgcgctggcg ggacgttccg 1140 gccgggacgt cgttagcagt gatcatcact gacgaatcga ggtcgaggaa ccgagcgtcc 1200 gaggaacaga ggcgcttatc ggttggccgc gagattcctg tcgatcctct cgtgcagcgc 1260 gattccgagg gaaacggaaa cgttgagaga ctcggtctgg ctcatcatgg ggatggaaac 1320 cgaggcggaa gacgcctcct cgaacaggtc ggaaggccca cccttttcgc tgccgaacag 1380 caaggccagc cgatccggat tgtccccgag ttccttcacg gaaatgtcgc catccgcctt 1440 gagcgtcatc agctgcatac cgctgtcccg aatgaaggcg atggcctcct cgcgaccgga 1500 gagaacgacg ggaagggaga agacgtaacc tcggctggcc ctttggagac gccggtccgc 1560 gatgctggtg atgtcactgt cgaccaggat gatccccgac gctccgagcg cgagcgacgt 1620 gcgtactatc gcgccgatgt tcccgacgat cttcaccccg tcgagaacga cgacgtcccc 1680 acgccggctc gcgatatcgc cgaacctggc cgggcgaggg acgcgggcga tgccgaatgt 1740 cttggccttc cgctccccct tgaacaactg gttgacgatc gaggagtcga tgaggcggac 1800 cggtatgttc tgccgcccgc acagatccag caactcagat ggaaaaggac tgctgtcgct 1860 gccgtagacc tcgatgaact ccaccccggc cgcgatgctg tgcatgaggg gctcgacgtc 1920 ctcgatcaac gttgtcttta tgttggatcg cgacggcttg gtgacatcga tgatccgctg 1980 caccgcggga tcggacggat ttgcgatggt gtccaactca gtcatggtcg tcctaccggc 2040 tgctgtgttc agtgacgcga ttcctggggt gtgacaccct acgcgacgat ggcggatggc 2100 tgccctgacc ggcaatcacc aacgcaaggg gaagtcgtcg ctctctggca aagctccccg 2160 ctcttccccg tccgggaccc gcgcggtcga tccccgcata tgaagtattc gccttgatca 2220 gggctctcgc ggacatcgga gaggtagccc agcaagcaga gacagcactg tgcgtgaacg 2280 cggtgaagac gaagtccttg aacccgaccg gcgaagatca cgcggaatgc cgtttacaag 2340 aggcttgccg ccgaggacaa atgacgacgc gcctgatgcg gtattttctc cttacgcatc 2400 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat 2460 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc 2520 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt 2580 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat 2640 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 2700 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 2760 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 2820 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 2880 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 2940 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 3000 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg 3060 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 3120 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 3180 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 3240 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 3300 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg 3360 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 3420 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 3480 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 3540 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 3600 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 3660 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 3720 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 3780 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 3840 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 3900 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 3960 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca 4020 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 4080 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 4140 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 4200 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 4260 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 4320 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 4380 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 4440 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 4500 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 4560 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac 4620 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc 4680 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg 4740 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat 4800 aacaatttca cacaggaaac agctatgacc atgattacgc caagcttgca tgcctgcagg 4860 tcgactctag aggatccccg ggtaccgagc tcgaattcac tggccgtcgt tttacaacgt 4920 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 4980 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 5040 ctgaatggcg aatggcgcgt cgaaggcccc ctgccagtgt cgggcggggg gccttttctg 5100 tgattccggg ccgaagaagt ctttacctaa agctgatcag cggggacccc tccgagagca 5160 agaaaggggg ccgagtgacc actaagggcg gtgagggatt ccatccctct cctttggtac 5220 aagtgtaaag gccccctttc atgctctcgc cccgctgatc acctttaggt aaacacttct 5280 ccaaccctaa gcggcattgc tttctgacgg gccgtcaggg gtttctgacg catcatcagg 5340 tgtgagtttc ttgactgccc agatttgatg agccgtgagg agcttgacga gcgcgggaaa 5400 gcctccgcgt tcgacggctt ccaggacgcg ggcttccagg ccgtgacgga tgaccatctt 5460 ccaagcggcc tccgccagct cggccacctc cccgccggcc acttcttcgg cggccaactc 5520 ttcctcggtc ttctcgtcca ctccgagcat ggctttcagc ttgggagacc accgcaacca 5580 ggacttcccc ttggtcgcct tctcgtactc cagccagaga tccacgacgg ccgcgtctcc 5640 ggtcgccatc aggtactcga tcatctcgaa cggcgtcagg ctgccgagct tgcctttctt 5700 gagatcaccg cgagcaactt cgttcccgat gtgcttgccg gaatcctgag ccttagcgat 5760 gtattccccg gcaccttcgg cggtcatgac cttgtcccac ttcacaccgt gagccttgtc 5820 ggcagggaag ccgtgcttct tcgtccaggc catccaaagc cgttgccagc gacccatcat 5880 gatggcgagt tcccgaacgt cggggacttc cttgaagcag acgatcacgt gaaggtgagg 5940 atgccaacca ttctttccgt aagtgatctc acgggaattg atggtgccga tctttcctgt 6000 tgccttcttt tcccggacgg ccataactcc ggtaccgagc tttcggaatg cgtcgttgag 6060 agcattaagc agatccgcca gagcgtgacc cctcgcgtgc cgaacggtga acgtgaccat 6120 ccaggcttcc ccaccaccca 6140 // ID AB041904; SV 1; linear; other DNA; STD; SYN; 720 BP. XX AC AB041904; XX DT 16-AUG-2000 (Rel. 64, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct ecgfp gene for enhanced cyan-green fluorescent protein, DE complete cds. XX KW Enhanced cyan-green fluorescent protein. XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-720 RA Miyawaki A., Sawano A.; RT ; RL Submitted (20-APR-2000) to the INSDC. RL Atsushi Miyawaki, ATDC, Brain Science Institute, RIKEN, Laboratory for Cell RL Function and Dynamics; 2-1 Hirosawa, Wako, Saitama 351-0198, Japan RL (E-mail:matsushi@brain.riken.go.jp, Tel:81-48-467-5917, Fax:81-48-467-5924) XX RN [2] RA Sawano A., Miyawaki A.; RT "Directed evolution of green fluorescent protein by a new versatile PCR RT strategy for site-directed and semi-random mutagenesis"; RL Nucleic Acids Res. 28:e78-e78(2000). XX DR MD5; 7ccd1289ab47403da25e8904b6d4e5ff. DR EuropePMC; PMC108465; 10931937. DR EuropePMC; PMC1360235; 16162177. XX FH Key Location/Qualifiers FH FT source 1..720 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..720 FT /codon_start=1 FT /transl_table=11 FT /gene="ecgfp" FT /product="enhanced cyan-green fluorescent protein" FT /note="originally from Aequorea victoria GFP" FT /protein_id="BAB11884.1" FT /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL FT KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD FT GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK FT ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL FT EFVTAAGITLGMDELYK" XX SQ Sequence 720 BP; 172 A; 242 C; 203 G; 103 T; 0 other; atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180 ctcgtgacca ccctgacctg gggcgtgcag tgcttcagcc gctaccccga ccacatgaag 240 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420 aagctggagt acaactacat cagccacaac gtctatatca ccgccgacaa gcagaagaac 480 ggcatcaagg ccaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa 720 // ID AB041927; SV 1; circular; other DNA; STD; SYN; 5883 BP. XX AC AB041927; XX DT 26-APR-2000 (Rel. 63, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 5) XX DE Retroviral vector pCXbsr DNA, complete sequence. XX KW . XX OS Retroviral vector pCXbsr OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-5883 RA Akagi T.; RT ; RL Submitted (17-APR-2000) to the INSDC. RL Tsuyoshi Akagi, Osaka Bioscience Institute, The First Department; 6-2-4 RL Furuedai, Suita, Osaka 565-0874, Japan (E-mail:takagi@obisun1.obi.or.jp, RL Tel:81-6-6872-4850, Fax:81-6-6871-6686) XX RN [2] RX DOI; 10.1073/pnas.140210297. RX PUBMED; 10852971. RA Akagi T., Shishido T., Murata K., Hanafusa H.; RT "v-Crk activates the phosphoinositide 3-kinase/AKT pathway in RT transformation"; RL Proc. Natl. Acad. Sci. U.S.A. 97(13):7290-7295(2000). XX DR MD5; 4405486cee0f595a6162598105d20d76. DR EuropePMC; PMC16538; 10852971. XX FH Key Location/Qualifiers FH FT source 1..5883 FT /organism="Retroviral vector pCXbsr" FT /mol_type="other DNA" FT /db_xref="taxon:197518" FT regulatory 1..466 FT /note="CMV enhancer" FT /regulatory_class="enhancer" FT repeat_region 467..762 FT /rpt_type=LONG_TERMINAL_REPEAT FT /note="R-U5 region of MuLV 5'LTR" FT misc_feature 763..1673 FT /note="psi+; extended viral packaging signal" FT misc_feature 1674..2049 FT /note="pol region containing splice accetor site" FT misc_feature 2052..2136 FT /note="multiple cloning site" FT regulatory 2137..2757 FT /note="IRES sequence from ECMV" FT /regulatory_class="ribosome_binding_site" FT exon 2759..3190 FT /gene="bsr" FT CDS 2759..3187 FT /codon_start=1 FT /transl_table=1 FT /gene="bsr" FT /function="blasticidin S resistance" FT /protein_id="BAA94845.1" FT /translation="MVMKTFNISQQDLELVEVATEKITMLYEDNKHHVGAAIRTKTGEI FT ISAVHIEAYIGRVTVCAEAIAIGSAVSNGQKDFDTIVAVRHPYSDEVDRSIRVVSPCGM FT CRELISDYAPDCFVLIEMNGKLVKTTIEELIPLKYTRN" FT repeat_region 3240..3832 FT /rpt_type=LONG_TERMINAL_REPEAT FT /note="3' LTR of MuLV" FT exon complement(4224..5682) FT /gene="bla" FT CDS complement(4822..5682) FT /codon_start=1 FT /transl_table=1 FT /gene="bla" FT /product="beta-lactamase" FT /function="ampicillin resistance" FT /protein_id="BAA94846.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" XX SQ Sequence 5883 BP; 1427 A; 1575 C; 1421 G; 1460 T; 0 other; tcgaggagct tggcccattg catacgttgt atccatatca taatatgtac atttatattg 60 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat 120 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 180 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 240 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gactatttac 300 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 360 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 420 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgcta gagaaccatc 480 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca 540 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc 600 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt 660 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt 720 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga 780 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac 840 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta 900 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac 960 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac 1020 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctaa taggagggat 1080 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc 1140 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt 1200 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatat gggcccgggc cagactgtta 1260 ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc 1320 agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct 1380 ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga 1440 tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct 1500 gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc 1560 cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc 1620 ctcgatcctc cctttatcca gccctcactc cttctctagg cgcccccata tggccatatg 1680 agatcttata tggggcaccc ccgccccttg taaacttccc tgaccctgac atgacaagag 1740 ttactaacag cccctctctc caagctcact tacaggctct ctacttagtc cagcacgaag 1800 tctggagacc tctggcggca gcctaccaag aacaactgga ccgaccggtg gtacctcacc 1860 cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac ctagaacctc 1920 gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa gtagacggca 1980 tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt ggaccatcct 2040 ctagactgcc ggatcccagt gtggtggtag ggaattcgag ctcggtaccc gggggcgcgc 2100 cggatcctta attaagcggc cgcggtaaca attgttaact aactaactaa gctagcaacg 2160 gtttccctct agcgggatca attccccctc tccctccccc ccccctaacg ttactggccg 2220 aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc 2280 gtcttttggc aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag 2340 gggtctttcc cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt 2400 tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa 2460 ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc 2520 aaaggcggca caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg 2580 gctctcctca agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat 2640 gggatctgat ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa 2700 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg ataataccat 2760 ggtcatgaaa acatttaaca tttctcaaca agatctagaa ttagtagaag tagcgacaga 2820 gaagattaca atgctttatg aggataataa acatcatgtg ggagcggcaa ttcgtacgaa 2880 aacaggagaa atcatttcgg cagtacatat tgaagcgtat ataggacgag taactgtttg 2940 tgcagaagcc attgcgattg gtagtgcagt ttcgaatgga caaaaggatt ttgacacgat 3000 tgtagctgtt agacaccctt attctgacga agtagataga agtattcgag tggtaagtcc 3060 ttgtggtatg tgtagggagt tgatttcaga ctatgcacca gattgttttg tgttaataga 3120 aatgaatggc aagttagtca aaactacgat tgaagaactc attccactca aatatacccg 3180 aaattaaaag tcgacacgat aaaataaaag attttattta gtctccagaa aaagggggga 3240 atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt ttggaaggca 3300 tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac agatggaaca 3360 gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca 3420 agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc 3480 cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca gcagtttcta 3540 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt 3600 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa 3660 taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg 3720 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc 3780 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc attctgcatt 3840 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 3900 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 3960 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 4020 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 4080 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 4140 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 4200 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 4260 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 4320 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 4380 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 4440 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 4500 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 4560 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 4620 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 4680 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 4740 caaaaaggat cttcacctag atccttttgc ggccggccgc aaatcaatct aaagtatata 4800 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 4860 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 4920 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 4980 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 5040 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 5100 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 5160 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 5220 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 5280 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 5340 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 5400 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca 5460 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 5520 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 5580 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 5640 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 5700 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 5760 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 5820 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 5880 tcc 5883 // ID AB041928; SV 1; circular; other DNA; STD; SYN; 6248 BP. XX AC AB041928; XX DT 26-APR-2000 (Rel. 63, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 4) XX DE Retroviral vector pCXneo DNA, complete sequence. XX KW . XX OS Retroviral vector pCXneo OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-6248 RA Akagi T.; RT ; RL Submitted (17-APR-2000) to the INSDC. RL Tsuyoshi Akagi, Osaka Bioscience Institute, The First Department; 6-2-4 RL Furuedai, Suita, Osaka 565-0874, Japan (E-mail:takagi@obisun1.obi.or.jp, RL Tel:81-6-6872-4850, Fax:81-6-6871-6686) XX RN [2] RX DOI; 10.1073/pnas.140210297. RX PUBMED; 10852971. RA Akagi T., Shishido T., Murata K., Hanafusa H.; RT "v-Crk activates the phosphoinositide 3-kinase/AKT pathway in RT transformation"; RL Proc. Natl. Acad. Sci. U.S.A. 97(13):7290-7295(2000). XX DR MD5; a906af7e9287f8b07239809da11377a8. DR EuropePMC; PMC16538; 10852971. XX FH Key Location/Qualifiers FH FT source 1..6248 FT /organism="Retroviral vector pCXneo" FT /mol_type="other DNA" FT /db_xref="taxon:197519" FT regulatory 1..466 FT /note="CMV enhancer" FT /regulatory_class="enhancer" FT repeat_region 467..762 FT /rpt_type=LONG_TERMINAL_REPEAT FT /note="R-U5 region of MuLV 5'LTR" FT misc_feature 763..1673 FT /note="psi+; MuLV extended packaging signal" FT misc_feature 1674..2049 FT /note="MuLV pol region containing splice acceptor site" FT misc_feature 2052..2136 FT /note="multiple cloning site" FT regulatory 2137..2757 FT /note="IRES sequence from ECMV" FT /regulatory_class="ribosome_binding_site" FT CDS 2751..3545 FT /codon_start=1 FT /transl_table=1 FT /gene="neo" FT /function="neomycin resistance" FT /protein_id="BAA94847.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT repeat_region 3605..4197 FT /rpt_type=LONG_TERMINAL_REPEAT FT /note="3' LTR of MuLV" FT CDS complement(5187..6047) FT /codon_start=1 FT /transl_table=1 FT /gene="bla" FT /product="beta-lactamase" FT /function="ampicillin resistance" FT /protein_id="BAA94848.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" XX SQ Sequence 6248 BP; 1420 A; 1733 C; 1579 G; 1516 T; 0 other; tcgaggagct tggcccattg catacgttgt atccatatca taatatgtac atttatattg 60 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat 120 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 180 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 240 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gactatttac 300 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 360 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 420 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatgcta gagaaccatc 480 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca 540 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc 600 cacaacccct cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt 660 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt 720 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atttgggggc tcgtccggga 780 tcgggagacc cctgcccagg gaccaccgac ccaccaccgg gaggtaagct ggccagcaac 840 ttatctgtgt ctgtccgatt gtctagtgtc tatgactgat tttatgcgcc tgcgtcggta 900 ctagttagct aactagctct gtatctggcg gacccgtggt ggaactgacg agttcggaac 960 acccggccgc aaccctggga gacgtcccag ggacttcggg ggccgttttt gtggcccgac 1020 ctgagtccaa aaatcccgat cgttttggac tctttggtgc acccccctaa taggagggat 1080 atgtggttct ggtaggagac gagaacctaa aacagttccc gcctccgtct gaatttttgc 1140 tttcggtttg ggaccgaagc cgcgccgcgc gtcttgtctg ctgcagcatc gttctgtgtt 1200 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatat gggcccgggc cagactgtta 1260 ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc gctcacaacc 1320 agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa tggccaacct 1380 ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc caggttaaga 1440 tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac atcgtgacct 1500 gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac cctaagcctc 1560 cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt tcgaccccgc 1620 ctcgatcctc cctttatcca gccctcactc cttctctagg cgcccccata tggccatatg 1680 agatcttata tggggcaccc ccgccccttg taaacttccc tgaccctgac atgacaagag 1740 ttactaacag cccctctctc caagctcact tacaggctct ctacttagtc cagcacgaag 1800 tctggagacc tctggcggca gcctaccaag aacaactgga ccgaccggtg gtacctcacc 1860 cttaccgagt cggcgacaca gtgtgggtcc gccgacacca gactaagaac ctagaacctc 1920 gctggaaagg accttacaca gtcctgctga ccacccccac cgccctcaaa gtagacggca 1980 tcgcagcttg gatacacgcc gcccacgtga aggctgccga ccccgggggt ggaccatcct 2040 ctagactgcc ggatcccagt gtggtggtag ggaattcgag ctcggtaccc gggggcgcgc 2100 cggatcctta attaagcggc cgcggtaaca attgttaact aactaactaa gctagcaacg 2160 gtttccctct agcgggatca attccgcccc ccccccctaa cgttactggc cgaagccgct 2220 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg 2280 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt 2340 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg 2400 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac 2460 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg 2520 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct 2580 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg 2640 atctggggcc tcggtgcaca tgctttacgt gtgtttagtc gaggttaaaa aacgtctagg 2700 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgataatacc atgattgaac 2760 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 2820 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 2880 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 2940 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 3000 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 3060 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 3120 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 3180 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 3240 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 3300 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 3360 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 3420 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 3480 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 3540 tctgagcggg actctggggt tcgataaaat aaaagatttt atttagtctc cagaaaaagg 3600 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgga 3660 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg 3720 gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag 3780 ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt 3840 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc cctcagcagt 3900 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct 3960 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag 4020 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg 4080 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct 4140 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcattct 4200 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 4260 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 4320 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 4380 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 4440 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 4500 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 4560 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 4620 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 4680 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 4740 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 4800 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 4860 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 4920 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 4980 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 5040 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 5100 attatcaaaa aggatcttca cctagatcct tttgcggccg gccgcaaatc aatctaaagt 5160 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5220 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5280 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5340 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 5400 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 5460 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 5520 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 5580 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 5640 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 5700 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 5760 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg 5820 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 5880 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 5940 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6000 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6060 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6120 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6180 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc 6240 tttcgtcc 6248 // ID AB042426; SV 1; circular; other DNA; STD; SYN; 5242 BP. XX AC AB042426; XX DT 20-JUL-2000 (Rel. 64, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 4) XX DE Shuttle vector pSET1 DNA, complete sequence. XX KW . XX OS Shuttle vector pSET1 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-5242 RA Takamatsu D., Osaki M., Sekizaki T.; RT ; RL Submitted (08-MAY-2000) to the INSDC. RL Daisuke Takamatsu, National Institute of Animal Health, Laboratory of RL Molecular Bacteriology; Kannondai 3-1-1, Tukuba, Ibaraki 305-0856, Japan RL (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, Fax:81-298-38-7743) XX RN [2] RX DOI; 10.1006/plas.2000.1510. RX PUBMED; 11322824. RA Takamatsu D., Osaki M., Sekizaki T.; RT "Construction and characterization of Streptococcus suis-Escherichia coli RT shuttle cloning vectors"; RL Plasmid 45(2):101-113(2001). XX DR MD5; 2429885ffd4569f2e35b5c21a1b1335d. XX FH Key Location/Qualifiers FH FT source 1..5242 FT /organism="Shuttle vector pSET1" FT /lab_host="Streptococcus suis, Escherichia coli" FT /mol_type="other DNA" FT /note="synonym:Shuttle Cloning Vector pSET1" FT /db_xref="taxon:125567" FT misc_feature 156..176 FT /gene="dso" FT /note="double-stranded replication origin" FT CDS 367..504 FT /codon_start=1 FT /transl_table=11 FT /gene="copG" FT /product="CopG" FT /function="A transcripsional repressor protein" FT /protein_id="BAB03235.1" FT /translation="MKKRLTITLSESVLENLEKMAKEMGLSKSALISVALENYKKGQVK FT " FT CDS 566..1246 FT /codon_start=1 FT /transl_table=11 FT /gene="repB" FT /product="RepB" FT /function="The plasmid-encoded initiator of replication. FT The protein introduces a strand- and site-specific nick in FT the double-stranded replication origin, on supercoiled FT DNA." FT /protein_id="BAB03236.1" FT /translation="MAKEKARYFTFLLYPESIPSDWELKLELLGVPIAVSPLHDRDKSD FT VEGQQYKKPHYHVIYVSKNPVTADSVRMKIKRSLGDNSVALVQIIRTSIENTYLYLTHE FT SKDAIEKKKHVYDKADITLLSNFDIDRYITLDVEEKDDMLNEVCDLIDEYDIANMRELR FT RFIKLHGAEHGLPSIKIINSVLRSHTGLIRLYFDAVYQERKYGSNIPNVNQETGEILSE FT DSDD" FT CDS complement(2101..2751) FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="chloramphenicol acetyl transferase" FT /function="chloramphenicol resistance" FT /protein_id="BAB03237.1" FT /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE FT GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV FT KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL FT LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL" FT CDS complement(3098..3421) FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha fragment" FT /protein_id="BAB03238.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS FT DAA" FT misc_feature 4337..4478 FT /gene="ssoA" FT /note="single-stranded replication origin" XX SQ Sequence 5242 BP; 1797 A; 861 C; 1101 G; 1483 T; 0 other; attaattttg aaatattaaa tatttttgga tatataggtg ttaagtttga aattattacc 60 gcttatgaaa atgggaaatt agttgaaacg caaattattg tggatgacat agagttgata 120 aaagttgaaa aattttttga ttaaagagaa gaagaggggg cgtactacga cccccctttt 180 aagtgccgag tgccaaattg ctaaaaaaag tgccgtaaag ctagatacat caagggttta 240 gctcatgcta cacttcggcg acattttggc gacattttcg gcgacaaaaa gaaaaaaata 300 cgataaatgt tgcaatgtat gcacagttat gctactatgg ttttataaat ttttgagagg 360 tggaacatga aaaaaagatt gacgataaca ctcagtgaat cggtgcttga aaatcttgaa 420 aaaatggcaa aagagatggg gttgtcaaaa tctgcactga tttcagttgc cttggaaaac 480 tacaagaaag gtcaagtaaa ataaaaaaaa gccgagctgg caggcgggct tttaaaagta 540 ttataacttg ggttaattat aacttatggc taaagaaaaa gcaagatact tcactttttt 600 actctatcct gagtcaatcc ctagtgattg ggaattgaaa ttagaattgc tgggcgtacc 660 tattgcagtc agccctttgc atgatagaga caagagtgat gttgagggac aacaatataa 720 aaaaccgcac tatcatgtga tttatgtttc aaaaaatcca gtcacggcag atagtgtgag 780 aatgaaaatt aagaggtcac ttggagataa tagcgttgct ctagttcaga taattagaac 840 tagcattgag aacacgtatt tatatcttac gcatgaatca aaggacgcta tcgaaaagaa 900 aaagcacgtc tatgataagg ctgacataac tttgcttagt aactttgata tagaccgtta 960 tataacgctt gacgtcgaag agaaagacga tatgctcaat gaggtttgtg accttattga 1020 tgagtatgat attgcaaata tgcgtgaatt gagacgtttt attaaacttc atggggctga 1080 acatggttta cctagtatca aaattataaa ttcagtttta cgttcgcata ctgggttaat 1140 acgtctgtat tttgatgctg tctatcaaga acgtaagtat ggcagtaata tccctaatgt 1200 taatcaagag acaggcgaga ttttaagtga ggatagcgat gactgataga aatgagctga 1260 ttaacgatat tgcagagttg aaagcaaaaa gagacagact actggctcaa atgaaagagg 1320 cggaacaatg ggaaagcgta gcgtgggata gttactatgc agtcgcagac catgtgaaag 1380 ctcttgagaa aaagcaggag ataggtcgaa actattggga aagttctcaa agagctatta 1440 gtcatcaatt tgactttgtg gctgaccaag ctaacaaagt caaaaaggtg ttggctaaaa 1500 agagatatga tttgctagat gaggaaatcg acaagctgat gaacgaagtt cgagagctgg 1560 cagatgtcct tgggattgag attgacgagc tccctcttga ttttcctttc ttcgctctaa 1620 gtgcagaggg ggtttctgat gagtaaaaca gtatcagaat tatccgcaga attaggggta 1680 agcaaacaat atttaaaccg tgttttaagt caaaataatc tcggtcgaaa aatcggaaac 1740 aaaaagttag tttccgatag tgacgtaaag tcactattat caatacttaa gaacaatatc 1800 ggaaaccaaa aatcggaaac tgaaaatata gtttccgata ttccgtcaaa gtcaactggt 1860 aaaagggata caggcgaaaa cggaaaccaa aaatcggaaa ctagtttccg ataattcgat 1920 gggttccgag gctcaacgtc aataaagcaa ttggaataaa gaagcgaaaa aggagaagtc 1980 ggttcagaaa aagaaggata tggatctgga gctgtaatat aaaaaccttc ttcaactaac 2040 ggggcaggtt agtgacatta gaaaaccgac tgtaaaaagt acagtcggca ttatctcata 2100 ttataaaagc cagtcattag gcctatctga caattcctga atagagttca taaacaatcc 2160 tgcatgataa ccatcacaaa cagaatgatg tacctgtaaa gatagcggta aatatattga 2220 attaccttta ttaatgaatt ttcctgctgt aataatgggt agaaggtaat tactattatt 2280 attgatattt aagttaaacc cagtaaatga agtccatgga ataatagaaa gagaaaaagc 2340 attttcaggt ataggtgttt tgggaaacaa tttccccgaa ccattatatt tctctacatc 2400 agaaaggtat aaatcataaa actctttgaa gtcattcttt acaggagtcc aaataccaga 2460 gaatgtttta gatacaccat caaaaattgt ataaagtggc tctaacttat cccaataacc 2520 taactctccg tcgctattgt aaccagttct aaaagctgta tttgagttta tcacccttgt 2580 cactaagaaa ataaatgcag ggtaaaattt atatccttct tgttttatgt ttcggtataa 2640 aacactaata tcaatttctg tggttatact aaaagtcgtt tgttggttca aataatgatt 2700 aaatatctct tttctcttcc aattgtctaa atcaatttta ttaaagttca tttgatatgc 2760 ctcctaaatt tttatctaaa gtgaatttag gaggcttact tgtctgcttt cttcattaga 2820 atcaatcctt ttttaaaagt caatattact gtaacataaa tatatatttt aaaaatatcc 2880 cactttatcc aattttcgtt tgttgaacca ttatatcaca ttatccatta aaaatcaaac 2940 aaattttcat caagctctag ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 3000 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 3060 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg 3120 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc 3180 gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 3240 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 3300 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgagctcg 3360 gtacccgggg atcctctaga gtcgacctgc aggcatgcaa gcttggcgta atcatggtca 3420 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 3480 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 3540 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 3600 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactaga 3660 aagcccctag aagacgaaat aaggactttg aatgaagttc ttaaggaaaa atacgaggag 3720 ttagacaaaa tcgagctacg agcctctgag agcctctcag agctatctgg tgtcgaaggg 3780 tatataaata acctagaaaa acattcaagc gccttagaaa gcaaaatagg gggcttagaa 3840 aacgaatatt taagactggc taaacaaaat gcccgattaa gcgacttgaa aatcatgtct 3900 gaaaaagaat tggctgaaat tcagccgaaa aaaggcatgt ttggaaaaga acatgtcgaa 3960 ttgactaaag agcaatttga gaaatttaag ggattgatat accgaagcaa gaatttagtt 4020 catgaaaagg aattagagaa tgaccaacta aggcggcaaa tgcctctgag aagctcaaaa 4080 aacggttttg aggcgagttt gcaacgggct aaggataaaa ctaaaggaga aagcatagac 4140 cgccttaaaa gcgaaattag agggcttaaa aatgaaaact cggttttgag acagcaaaat 4200 gacaaaatgc taggtaagtt aagagagttt atgcctgata aagcgttaaa taattttatt 4260 tctgaactta aatcaataaa acctatcgtg aagatagtta aaagagttat tgaaaaaggc 4320 ttagggctct aagcgattta tgccgagaaa actcttgcta ggaagctatg cgaaatagac 4380 tagtaggggt cgagtcgaca ggctgaaagc ttgccgaccg aacacgacag tcagatttca 4440 gcataggttt cttcctagca agaggaaatt ggaataaaga aagcgtatca aagtaaaact 4500 aaaaggacta agtcctgtgt aatacaggcc ttagtccttt acttaaaata gaatgtccaa 4560 ctttttgggg tcagtatata ttttagtctt ttttattcta tagttgggat agagatttgt 4620 actcctcctg cgccatatgc aattccgctt atacctgcat ttcttgcttt tttaggtaaa 4680 ccgaatgaac cactaactga ttgacctcct ttgccagatt tgctgacagg atatattcta 4740 gtagtgttat ctgagaaagt aactgttatc aatccagaat atgtataact ttttgatgta 4800 ttaggtttaa ctgaccacca aacttctcga ccaccaccat ataattcgat ttttgtatat 4860 ccgccatttc cgtaacttgt tttgtaagga actatttcac cggtataaga ataatcagtt 4920 gtagttgaaa tatttgtatt tgaggtagtg actgtattat ctgcaaaaac agtagaaatg 4980 gatataaatc ctaacataga aaaaagagtg acaaatacaa ttattgattt tttgaaactt 5040 ttcatgaaag tttacctccg tttttttgtt ataattatag caaaaaaata attaaaaaga 5100 aagcgttatc atagaaatga aaaagaattt tattgcaatt ataaaaaaca tattaaaaaa 5160 caaaaatatc cctaaatatt gttttgaaat tattgaaaaa gatggaagta cttgttttct 5220 ttcgacaaat tactttgatg ta 5242 // ID AB042430; SV 1; circular; other DNA; STD; SYN; 5016 BP. XX AC AB042430; XX DT 20-JUL-2000 (Rel. 64, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 4) XX DE Shuttle vector pSET2 DNA, complete sequence. XX KW . XX OS Shuttle vector pSET2 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-5016 RA Takamatsu D., Osaki M., Sekizaki T.; RT ; RL Submitted (08-MAY-2000) to the INSDC. RL Daisuke Takamatsu, National Institute of Animal Health, Laboratory of RL Molecular Bacteriology; Kannondai 3-1-1, Tukuba, Ibaraki 305-0856, Japan RL (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, Fax:81-298-38-7743) XX RN [2] RX DOI; 10.1006/plas.2000.1510. RX PUBMED; 11322824. RA Takamatsu D., Osaki M., Sekizaki T.; RT "Construction and characterization of Streptococcus suis-Escherichia coli RT shuttle cloning vectors"; RL Plasmid 45(2):101-113(2001). XX DR MD5; 7c8792b173d033525c98b15affa8caff. XX FH Key Location/Qualifiers FH FT source 1..5016 FT /organism="Shuttle vector pSET2" FT /lab_host="Streptococcus suis, Escherichia coli" FT /mol_type="other DNA" FT /note="synonym:Shuttle Cloning Vector pSET2" FT /db_xref="taxon:125568" FT misc_feature 156..176 FT /gene="dso" FT /note="double-stranded replication origin" FT CDS 367..504 FT /codon_start=1 FT /transl_table=11 FT /gene="copG" FT /product="CopG" FT /function="transcriptional repressor protein" FT /protein_id="BAB03239.1" FT /translation="MKKRLTITLSESVLENLEKMAKEMGLSKSALISVALENYKKGQVK FT " FT CDS 566..1246 FT /codon_start=1 FT /transl_table=11 FT /gene="repB" FT /product="RepB" FT /function="The plasmid-encoded initiator of replication. FT The protein introduces a strand- and site-specific nick in FT the double-stranded replication origin, on supercoiled FT DNA." FT /protein_id="BAB03240.1" FT /translation="MAKEKARYFTFLLYPESIPSDWELKLELLGVPIAVSPLHDRDKSD FT VEGQQYKKPHYHVIYVSKNPVTADSVRMKIKRSLGDNSVALVQIIRTSIENTYLYLTHE FT SKDAIEKKKHVYDKADITLLSNFDIDRYITLDVEEKDDMLNEVCDLIDEYDIANMRELR FT RFIKLHGAEHGLPSIKIINSVLRSHTGLIRLYFDAVYQERKYGSNIPNVNQETGEILSE FT DSDD" FT CDS complement(1703..2470) FT /codon_start=1 FT /transl_table=11 FT /gene="add9" FT /product="spectinomycin adenyltransferase" FT /function="spectinomycin resistance" FT /protein_id="BAB03241.1" FT /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS FT DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ FT EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR FT RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI FT LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL" FT CDS complement(2872..3195) FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha fragment" FT /protein_id="BAB03242.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS FT DAA" FT misc_feature 4111..4252 FT /gene="ssoA" FT /note="single-stranded replication origin" XX SQ Sequence 5016 BP; 1658 A; 811 C; 1018 G; 1529 T; 0 other; attaattttg aaatattaaa tatttttgga tatataggtg ttaagtttga aattattacc 60 gcttatgaaa atgggaaatt agttgaaacg caaattattg tggatgacat agagttgata 120 aaagttgaaa aattttttga ttaaagagaa gaagaggggg cgtactacga cccccctttt 180 aagtgccgag tgccaaattg ctaaaaaaag tgccgtaaag ctagatacat caagggttta 240 gctcatgcta cacttcggcg acattttggc gacattttcg gcgacaaaaa gaaaaaaata 300 cgataaatgt tgcaatgtat gcacagttat gctactatgg ttttataaat ttttgagagg 360 tggaacatga aaaaaagatt gacgataaca ctcagtgaat cggtgcttga aaatcttgaa 420 aaaatggcaa aagagatggg gttgtcaaaa tctgcactga tttcagttgc cttggaaaac 480 tacaagaaag gtcaagtaaa ataaaaaaaa gccgagctgg caggcgggct tttaaaagta 540 ttataacttg ggttaattat aacttatggc taaagaaaaa gcaagatact tcactttttt 600 actctatcct gagtcaatcc ctagtgattg ggaattgaaa ttagaattgc tgggcgtacc 660 tattgcagtc agccctttgc atgatagaga caagagtgat gttgagggac aacaatataa 720 aaaaccgcac tatcatgtga tttatgtttc aaaaaatcca gtcacggcag atagtgtgag 780 aatgaaaatt aagaggtcac ttggagataa tagcgttgct ctagttcaga taattagaac 840 tagcattgag aacacgtatt tatatcttac gcatgaatca aaggacgcta tcgaaaagaa 900 aaagcacgtc tatgataagg ctgacataac tttgcttagt aactttgata tagaccgtta 960 tataacgctt gacgtcgaag agaaagacga tatgctcaat gaggtttgtg accttattga 1020 tgagtatgat attgcaaata tgcgtgaatt gagacgtttt attaaacttc atggggctga 1080 acatggttta cctagtatca aaattataaa ttcagtttta cgttcgcata ctgggttaat 1140 acgtctgtat tttgatgctg tctatcaaga acgtaagtat ggcagtaata tccctaatgt 1200 taatcaagag acaggcgaga ttttaagtga ggatagcgat gactgataga aatgagctga 1260 ttaacgatat tgcagagttg aaagcaaaaa gagacagact actggctcaa atgaaagagg 1320 cggaacaatg ggaaagcgta gcgtgggata gttactatgc agtcgcagac catgtgaaag 1380 ctcttgagaa aaagcaggag ataggtcgaa actattggga aagttctcaa agagctatta 1440 gtcatcaatt tgactttgtg gctgaccaag ctaacaaagt caaaaaggtg ttggctaaaa 1500 agagatatga tttgctagat gaggaaatcg acaagctgat gaacgaagtt cgagagctgg 1560 cagatgtcct tgggattgag attgacgagc tcggtttatt gttttctaaa atctgattac 1620 caattagaat gaatatttcc caaatattaa ataataaaac aaaaaaattg aaaaaagtgt 1680 ttccaccatt ttttcaattt ttttataatt tttttaatct gttatttaaa tagtttatag 1740 ttaaatttac attttcatta gtccattcaa tattctctcc aagataacta cgaactgcta 1800 acaaaattct ctccctatgt tctaatggag aagattcagc cactgcattt cccgcaatat 1860 cttttggtat gattttaccc gtgtccatag ttaaaatcat acggcataaa gttaatatag 1920 agttggtttc atcatcctga taattatcta ttaattcctc tgacgaatcc ataatggctc 1980 ttctcacatc agaaaatgga atatcaggta gtaattcctc taagtcataa tttccgtata 2040 ttcttttatt ttttcgtttt gcttggtaaa gcattatggt taaatctgaa tttaattcct 2100 tctgaggaat gtatccttgt tcataaagct cttgtaacca ttctccataa ataaattctt 2160 gtttgggagg atgattccac ggtaccattt cttgctgaat aataattgtt aattcaatat 2220 atcgtaagtt gcttttatct cctatttttt ttgaaatagg tctaattttt tgtataagta 2280 tttctttact ttgatctgtc aatggttcag atacgacgac taaaaagtca agatcactat 2340 ttggttttag tccactctca actcctgatc caaacatgta agtaccaata aggttatttt 2400 ttaaatgttt ccgaagtatt tttttcactt tattaatttg ttcgtatgta ttcaaatata 2460 tcctcctcac tattttgatt agtacctatt ttatatccat agttgttaat taaataaact 2520 taatttagtt tatttataga tttcattggc ttctaaattt tttatctaga taataattat 2580 tttagttaat tttattctag attatatatg atatgatctt tcatttccat aaaactaaag 2640 taagtgtaaa cctattcatt gttttaaaaa tatctcttgc cagtcacgtt acgttattag 2700 ttatagttat tataacatgt attcacgaac agatctcggt gatgacggtg aaaacctctg 2760 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca 2820 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc 2880 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt 2940 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg 3000 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag 3060 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag 3120 tgaattcgag ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg 3180 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 3240 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 3300 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 3360 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 3420 cctcgctcac tagaaagccc ctagaagacg aaataaggac tttgaatgaa gttcttaagg 3480 aaaaatacga ggagttagac aaaatcgagc tacgagcctc tgagagcctc tcagagctat 3540 ctggtgtcga agggtatata aataacctag aaaaacattc aagcgcctta gaaagcaaaa 3600 tagggggctt agaaaacgaa tatttaagac tggctaaaca aaatgcccga ttaagcgact 3660 tgaaaatcat gtctgaaaaa gaattggctg aaattcagcc gaaaaaaggc atgtttggaa 3720 aagaacatgt cgaattgact aaagagcaat ttgagaaatt taagggattg atataccgaa 3780 gcaagaattt agttcatgaa aaggaattag agaatgacca actaaggcgg caaatgcctc 3840 tgagaagctc aaaaaacggt tttgaggcga gtttgcaacg ggctaaggat aaaactaaag 3900 gagaaagcat agaccgcctt aaaagcgaaa ttagagggct taaaaatgaa aactcggttt 3960 tgagacagca aaatgacaaa atgctaggta agttaagaga gtttatgcct gataaagcgt 4020 taaataattt tatttctgaa cttaaatcaa taaaacctat cgtgaagata gttaaaagag 4080 ttattgaaaa aggcttaggg ctctaagcga tttatgccga gaaaactctt gctaggaagc 4140 tatgcgaaat agactagtag gggtcgagtc gacaggctga aagcttgccg accgaacacg 4200 acagtcagat ttcagcatag gtttcttcct agcaagagga aattggaata aagaaagcgt 4260 atcaaagtaa aactaaaagg actaagtcct gtgtaataca ggccttagtc ctttacttaa 4320 aatagaatgt ccaacttttt ggggtcagta tatattttag tcttttttat tctatagttg 4380 ggatagagat ttgtactcct cctgcgccat atgcaattcc gcttatacct gcatttcttg 4440 cttttttagg taaaccgaat gaaccactaa ctgattgacc tcctttgcca gatttgctga 4500 caggatatat tctagtagtg ttatctgaga aagtaactgt tatcaatcca gaatatgtat 4560 aactttttga tgtattaggt ttaactgacc accaaacttc tcgaccacca ccatataatt 4620 cgatttttgt atatccgcca tttccgtaac ttgttttgta aggaactatt tcaccggtat 4680 aagaataatc agttgtagtt gaaatatttg tatttgaggt agtgactgta ttatctgcaa 4740 aaacagtaga aatggatata aatcctaaca tagaaaaaag agtgacaaat acaattattg 4800 attttttgaa acttttcatg aaagtttacc tccgtttttt tgttataatt atagcaaaaa 4860 aataattaaa aagaaagcgt tatcatagaa atgaaaaaga attttattgc aattataaaa 4920 aacatattaa aaaacaaaaa tatccctaaa tattgttttg aaattattga aaaagatgga 4980 agtacttgtt ttctttcgac aaattacttt gatgta 5016 // ID AB042431; SV 1; circular; other DNA; STD; SYN; 6378 BP. XX AC AB042431; XX DT 20-JUL-2000 (Rel. 64, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 4) XX DE Shuttle vector pSET3 DNA, complete sequence. XX KW . XX OS Shuttle vector pSET3 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-6378 RA Takamatsu D., Osaki M., Sekizaki T.; RT ; RL Submitted (08-MAY-2000) to the INSDC. RL Daisuke Takamatsu, National Institute of Animal Health, Laboratory of RL Molecular Bacteriology; Kannondai 3-1-1, Tukuba, Ibaraki 305-0856, Japan RL (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, Fax:81-298-38-7743) XX RN [2] RX DOI; 10.1006/plas.2000.1510. RX PUBMED; 11322824. RA Takamatsu D., Osaki M., Sekizaki T.; RT "Construction and characterization of Streptococcus suis-Escherichia coli RT shuttle cloning vectors"; RL Plasmid 45(2):101-113(2001). XX DR MD5; fa8cfffb06e34597db6bc0ed01acd62f. XX FH Key Location/Qualifiers FH FT source 1..6378 FT /organism="Shuttle vector pSET3" FT /lab_host="Streptococcus suis, Escherichia coli" FT /mol_type="other DNA" FT /note="synonym:Shuttle Cloning Vector pSET3" FT /db_xref="taxon:125569" FT misc_feature 156..176 FT /gene="dso" FT /note="double-stranded replication origin" FT CDS 367..504 FT /codon_start=1 FT /transl_table=11 FT /gene="copG" FT /product="CopG" FT /function="transcripsional repressor protein" FT /protein_id="BAB03243.1" FT /translation="MKKRLTITLSESVLENLEKMAKEMGLSKSALISVALENYKKGQVK FT " FT CDS 566..1246 FT /codon_start=1 FT /transl_table=11 FT /gene="repB" FT /product="RepB" FT /function="The plasmid-encoded initiator of replication. FT The protein introduces a strand- and site-specific nick in FT the double-stranded replication origin, on supercoiled FT DNA." FT /protein_id="BAB03244.1" FT /translation="MAKEKARYFTFLLYPESIPSDWELKLELLGVPIAVSPLHDRDKSD FT VEGQQYKKPHYHVIYVSKNPVTADSVRMKIKRSLGDNSVALVQIIRTSIENTYLYLTHE FT SKDAIEKKKHVYDKADITLLSNFDIDRYITLDVEEKDDMLNEVCDLIDEYDIANMRELR FT RFIKLHGAEHGLPSIKIINSVLRSHTGLIRLYFDAVYQERKYGSNIPNVNQETGEILSE FT DSDD" FT CDS complement(2101..2751) FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="chloramphenicol acetyl transferase" FT /function="chloramphenicol resistance" FT /protein_id="BAB03245.1" FT /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE FT GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV FT KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL FT LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL" FT CDS complement(3064..3831) FT /codon_start=1 FT /transl_table=11 FT /gene="add9" FT /product="spectinomycin adenyltransferase" FT /function="spectinomycin resistance" FT /protein_id="BAB03246.1" FT /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS FT DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ FT EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR FT RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI FT LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL" FT CDS complement(4234..4557) FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="beta-galactosidase alpha fragment" FT /protein_id="BAB03247.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS FT DAA" FT misc_feature 5473..5614 FT /gene="ssoA" FT /note="single-stranded replication origin" XX SQ Sequence 6378 BP; 2152 A; 1036 C; 1233 G; 1957 T; 0 other; attaattttg aaatattaaa tatttttgga tatataggtg ttaagtttga aattattacc 60 gcttatgaaa atgggaaatt agttgaaacg caaattattg tggatgacat agagttgata 120 aaagttgaaa aattttttga ttaaagagaa gaagaggggg cgtactacga cccccctttt 180 aagtgccgag tgccaaattg ctaaaaaaag tgccgtaaag ctagatacat caagggttta 240 gctcatgcta cacttcggcg acattttggc gacattttcg gcgacaaaaa gaaaaaaata 300 cgataaatgt tgcaatgtat gcacagttat gctactatgg ttttataaat ttttgagagg 360 tggaacatga aaaaaagatt gacgataaca ctcagtgaat cggtgcttga aaatcttgaa 420 aaaatggcaa aagagatggg gttgtcaaaa tctgcactga tttcagttgc cttggaaaac 480 tacaagaaag gtcaagtaaa ataaaaaaaa gccgagctgg caggcgggct tttaaaagta 540 ttataacttg ggttaattat aacttatggc taaagaaaaa gcaagatact tcactttttt 600 actctatcct gagtcaatcc ctagtgattg ggaattgaaa ttagaattgc tgggcgtacc 660 tattgcagtc agccctttgc atgatagaga caagagtgat gttgagggac aacaatataa 720 aaaaccgcac tatcatgtga tttatgtttc aaaaaatcca gtcacggcag atagtgtgag 780 aatgaaaatt aagaggtcac ttggagataa tagcgttgct ctagttcaga taattagaac 840 tagcattgag aacacgtatt tatatcttac gcatgaatca aaggacgcta tcgaaaagaa 900 aaagcacgtc tatgataagg ctgacataac tttgcttagt aactttgata tagaccgtta 960 tataacgctt gacgtcgaag agaaagacga tatgctcaat gaggtttgtg accttattga 1020 tgagtatgat attgcaaata tgcgtgaatt gagacgtttt attaaacttc atggggctga 1080 acatggttta cctagtatca aaattataaa ttcagtttta cgttcgcata ctgggttaat 1140 acgtctgtat tttgatgctg tctatcaaga acgtaagtat ggcagtaata tccctaatgt 1200 taatcaagag acaggcgaga ttttaagtga ggatagcgat gactgataga aatgagctga 1260 ttaacgatat tgcagagttg aaagcaaaaa gagacagact actggctcaa atgaaagagg 1320 cggaacaatg ggaaagcgta gcgtgggata gttactatgc agtcgcagac catgtgaaag 1380 ctcttgagaa aaagcaggag ataggtcgaa actattggga aagttctcaa agagctatta 1440 gtcatcaatt tgactttgtg gctgaccaag ctaacaaagt caaaaaggtg ttggctaaaa 1500 agagatatga tttgctagat gaggaaatcg acaagctgat gaacgaagtt cgagagctgg 1560 cagatgtcct tgggattgag attgacgagc tccctcttga ttttcctttc ttcgctctaa 1620 gtgcagaggg ggtttctgat gagtaaaaca gtatcagaat tatccgcaga attaggggta 1680 agcaaacaat atttaaaccg tgttttaagt caaaataatc tcggtcgaaa aatcggaaac 1740 aaaaagttag tttccgatag tgacgtaaag tcactattat caatacttaa gaacaatatc 1800 ggaaaccaaa aatcggaaac tgaaaatata gtttccgata ttccgtcaaa gtcaactggt 1860 aaaagggata caggcgaaaa cggaaaccaa aaatcggaaa ctagtttccg ataattcgat 1920 gggttccgag gctcaacgtc aataaagcaa ttggaataaa gaagcgaaaa aggagaagtc 1980 ggttcagaaa aagaaggata tggatctgga gctgtaatat aaaaaccttc ttcaactaac 2040 ggggcaggtt agtgacatta gaaaaccgac tgtaaaaagt acagtcggca ttatctcata 2100 ttataaaagc cagtcattag gcctatctga caattcctga atagagttca taaacaatcc 2160 tgcatgataa ccatcacaaa cagaatgatg tacctgtaaa gatagcggta aatatattga 2220 attaccttta ttaatgaatt ttcctgctgt aataatgggt agaaggtaat tactattatt 2280 attgatattt aagttaaacc cagtaaatga agtccatgga ataatagaaa gagaaaaagc 2340 attttcaggt ataggtgttt tgggaaacaa tttccccgaa ccattatatt tctctacatc 2400 agaaaggtat aaatcataaa actctttgaa gtcattcttt acaggagtcc aaataccaga 2460 gaatgtttta gatacaccat caaaaattgt ataaagtggc tctaacttat cccaataacc 2520 taactctccg tcgctattgt aaccagttct aaaagctgta tttgagttta tcacccttgt 2580 cactaagaaa ataaatgcag ggtaaaattt atatccttct tgttttatgt ttcggtataa 2640 aacactaata tcaatttctg tggttatact aaaagtcgtt tgttggttca aataatgatt 2700 aaatatctct tttctcttcc aattgtctaa atcaatttta ttaaagttca tttgatatgc 2760 ctcctaaatt tttatctaaa gtgaatttag gaggcttact tgtctgcttt cttcattaga 2820 atcaatcctt ttttaaaagt caatattact gtaacataaa tatatatttt aaaaatatcc 2880 cactttatcc aattttcgtt tgttgaacca ttatatcaca ttatccatta aaaatcaaac 2940 aaattttcat caagctctag tgttttctaa aatctgatta ccaattagaa tgaatatttc 3000 ccaaatatta aataataaaa caaaaaaatt gaaaaaagtg tttccaccat tttttcaatt 3060 tttttataat ttttttaatc tgttatttaa atagtttata gttaaattta cattttcatt 3120 agtccattca atattctctc caagataact acgaactgct aacaaaattc tctccctatg 3180 ttctaatgga gaagattcag ccactgcatt tcccgcaata tcttttggta tgattttacc 3240 cgtgtccata gttaaaatca tacggcataa agttaatata gagttggttt catcatcctg 3300 ataattatct attaattcct ctgacgaatc cataatggct cttctcacat cagaaaatgg 3360 aatatcaggt agtaattcct ctaagtcata atttccgtat attcttttat tttttcgttt 3420 tgcttggtaa agcattatgg ttaaatctga atttaattcc ttctgaggaa tgtatccttg 3480 ttcataaagc tcttgtaacc attctccata aataaattct tgtttgggag gatgattcca 3540 cggtaccatt tcttgctgaa taataattgt taattcaata tatcgtaagt tgcttttatc 3600 tcctattttt tttgaaatag gtctaatttt ttgtataagt atttctttac tttgatctgt 3660 caatggttca gatacgacga ctaaaaagtc aagatcacta tttggtttta gtccactctc 3720 aactcctgat ccaaacatgt aagtaccaat aaggttattt tttaaatgtt tccgaagtat 3780 ttttttcact ttattaattt gttcgtatgt attcaaatat atcctcctca ctattttgat 3840 tagtacctat tttatatcca tagttgttaa ttaaataaac ttaatttagt ttatttatag 3900 atttcattgg cttctaaatt ttttatctag ataataatta ttttagttaa ttttattcta 3960 gattatatat gatatgatct ttcatttcca taaaactaaa gtaagtgtaa acctattcat 4020 tgttttaaaa atatctcttg ccagtcacgt tacgttatta gttatagtta ttataacatg 4080 tattcacgaa cactagttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 4140 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 4200 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 4260 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 4320 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc 4380 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac 4440 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgaattcg agctcggtac 4500 ccggggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc 4560 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca 4620 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct 4680 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 4740 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actagaaagc 4800 ccctagaaga cgaaataagg actttgaatg aagttcttaa ggaaaaatac gaggagttag 4860 acaaaatcga gctacgagcc tctgagagcc tctcagagct atctggtgtc gaagggtata 4920 taaataacct agaaaaacat tcaagcgcct tagaaagcaa aatagggggc ttagaaaacg 4980 aatatttaag actggctaaa caaaatgccc gattaagcga cttgaaaatc atgtctgaaa 5040 aagaattggc tgaaattcag ccgaaaaaag gcatgtttgg aaaagaacat gtcgaattga 5100 ctaaagagca atttgagaaa tttaagggat tgatataccg aagcaagaat ttagttcatg 5160 aaaaggaatt agagaatgac caactaaggc ggcaaatgcc tctgagaagc tcaaaaaacg 5220 gttttgaggc gagtttgcaa cgggctaagg ataaaactaa aggagaaagc atagaccgcc 5280 ttaaaagcga aattagaggg cttaaaaatg aaaactcggt tttgagacag caaaatgaca 5340 aaatgctagg taagttaaga gagtttatgc ctgataaagc gttaaataat tttatttctg 5400 aacttaaatc aataaaacct atcgtgaaga tagttaaaag agttattgaa aaaggcttag 5460 ggctctaagc gatttatgcc gagaaaactc ttgctaggaa gctatgcgaa atagactagt 5520 aggggtcgag tcgacaggct gaaagcttgc cgaccgaaca cgacagtcag atttcagcat 5580 aggtttcttc ctagcaagag gaaattggaa taaagaaagc gtatcaaagt aaaactaaaa 5640 ggactaagtc ctgtgtaata caggccttag tcctttactt aaaatagaat gtccaacttt 5700 ttggggtcag tatatatttt agtctttttt attctatagt tgggatagag atttgtactc 5760 ctcctgcgcc atatgcaatt ccgcttatac ctgcatttct tgctttttta ggtaaaccga 5820 atgaaccact aactgattga cctcctttgc cagatttgct gacaggatat attctagtag 5880 tgttatctga gaaagtaact gttatcaatc cagaatatgt ataacttttt gatgtattag 5940 gtttaactga ccaccaaact tctcgaccac caccatataa ttcgattttt gtatatccgc 6000 catttccgta acttgttttg taaggaacta tttcaccggt ataagaataa tcagttgtag 6060 ttgaaatatt tgtatttgag gtagtgactg tattatctgc aaaaacagta gaaatggata 6120 taaatcctaa catagaaaaa agagtgacaa atacaattat tgattttttg aaacttttca 6180 tgaaagttta cctccgtttt tttgttataa ttatagcaaa aaaataatta aaaagaaagc 6240 gttatcatag aaatgaaaaa gaattttatt gcaattataa aaaacatatt aaaaaacaaa 6300 aatatcccta aatattgttt tgaaattatt gaaaaagatg gaagtacttg ttttctttcg 6360 acaaattact ttgatgta 6378 // ID AB043474; SV 1; circular; other DNA; STD; SYN; 8620 BP. XX AC AB043474; XX DT 30-MAY-2000 (Rel. 63, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 3) XX DE Shuttle vector pNIT6010 DNA, complete sequence. XX KW . XX OS Shuttle vector pNIT6010 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-8620 RA Itoh Y., Nishijyo T.; RT ; RL Submitted (22-MAY-2000) to the INSDC. RL Yoshifumi Itoh, National Food Research Institute, Division of Applied RL Microbiology; kannondai 2-1-2, Tsukuba, Ibaraki 305-8642, Japan RL (E-mail:yosifumi@nfri.affrc.go.jp, Tel:81-298-38-8075, Fax:81-298-38-7996) XX RN [2] RX DOI; 10.1094/MPMI.2000.13.2.232. RX PUBMED; 10659714. RA Heeb S., Itoh Y., Nishijyo T., Schnider U., Keel C., Wade J., Walsh U., RA OGara F., Haas D.; RT "Small, stable shuttle vectors based on the minimal pVS1 replicon for use RT in gram-negative, plant-associated bacteria"; RL Mol. Plant Microbe Interact. 13(2):232-237(2000). XX DR MD5; c416a7b9f3433dd7388ede7593692bc3. XX FH Key Location/Qualifiers FH FT source 1..8620 FT /organism="Shuttle vector pNIT6010" FT /lab_host="Escherichia coli, Pseudomonas aeruginosa" FT /mol_type="other DNA" FT /db_xref="taxon:126707" FT CDS 195..881 FT /codon_start=1 FT /transl_table=11 FT /product="pVS1 resolvase" FT /function="unknown" FT /note="not required for stable maintenance" FT /note="orf1" FT /protein_id="BAA96330.1" FT /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT FT RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT FT TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI FT DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE FT RQEEQA" FT CDS 878..1093 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="not required for stable maintenance" FT /note="orf2" FT /protein_id="BAA96331.1" FT /translation="MKPHQDGQDEPFFITEEIEAEMIAAGYVFEPPAHVSTVRLHEILA FT GLSDAKLAAWPASLAAEETERRRLKR" FT CDS 1180..1809 FT /codon_start=1 FT /transl_table=11 FT /gene="staA" FT /product="StaA" FT /function="pVS1 partitioning protein" FT /note="similar to IncC2" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="BAA96332.1" FT /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR FT DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP FT VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI FT LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" FT CDS 1830..2045 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="not required for stable mainenance" FT /note="orf3" FT /protein_id="BAA96333.1" FT /translation="MSKSTNTLSAGRPSARSSKAATLASLADTPAMKRVNFQLPAEDHT FT KLKMYAVRQGKTITELLSEYIAQLPE" FT misc_feature 2076..2088 FT /note="KorB box" FT CDS 2238..3311 FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="RepA" FT /function="pVS1 replication protein" FT /protein_id="BAA96334.1" FT /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW FT QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS FT KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP FT GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE FT ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR FT LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI FT LVMRYRNLIEGEASAGS" FT misc_difference 2974 FT /replace="t" FT /note="present in pME294, pME6012, pNIC6012 and pNIT6012" FT /note="results in increased copy number" FT misc_difference 3015 FT /replace="a" FT /note="present in pME292, pME6011, pNIC6011 and pNIT6011" FT /note="results in decreased copy mumber" FT rep_origin 3377..3571 FT /note="pVS1 origin of replication" FT misc_feature 3377..3384 FT /note="DnaA box" FT repeat_region 3388..3471 FT /rpt_type=DIRECT FT /note="21 bp" FT misc_feature 3472..3563 FT /note="AT-rich region" FT misc_feature 3564..3571 FT /note="DnaA box" FT rep_origin 4264..5093 FT /note="p15A origin of replication" FT misc_feature 5068..5089 FT /note="p15A origin of transfer" FT misc_feature 5450..5502 FT /note="multiple cloning site" FT CDS complement(6289..6966) FT /codon_start=1 FT /transl_table=11 FT /gene="tetR" FT /product="RK2 tetracycline repressor protein TetR" FT /protein_id="BAA96335.1" FT /translation="MFISDKVSSMTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGV FT QQPALYWHFRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRD FT GARIHAGTRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSD FT AGERGGTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRK FT GDD" FT CDS 6970..8244 FT /codon_start=1 FT /transl_table=11 FT /gene="tetA" FT /product="RK2 tetracycline resistance protein TetA" FT /protein_id="BAA96336.1" FT /translation="MSTNLSVIKNPRVQSDQRRLVRRPDVKPNIPLIVILSTVALDAVG FT IGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALVQFACAPVLGALSDRFGRRPILLVS FT LAGATVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSA FT CFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREAL FT NPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFG FT ILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGI FT GMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAG FT AALYLLCLPALRRGLWSGAGQRADR" FT misc_feature 8247..8620 FT /note="RP4 mob region" XX SQ Sequence 8620 BP; 1855 A; 2470 C; 2513 G; 1782 T; 0 other; agatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt 60 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt 120 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc 180 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca 240 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca 300 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc 360 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca 420 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta 480 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt 540 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg 600 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga 660 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac 720 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc 780 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc 840 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca 900 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt 960 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc 1020 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg 1080 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat 1140 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt 1200 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa 1260 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg 1320 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac 1380 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg 1440 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca 1500 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag 1560 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc 1620 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt 1680 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca 1740 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa 1800 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg 1860 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag 1920 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg 1980 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag 2040 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg 2100 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc 2160 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc 2220 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg 2280 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca 2340 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag 2400 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg 2460 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca 2520 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc 2580 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg 2640 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat 2700 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac 2760 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca 2820 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc 2880 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa 2940 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag 3000 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca 3060 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt 3120 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca 3180 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg 3240 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg 3300 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc 3360 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga 3420 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag 3480 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta 3540 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc 3600 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc 3660 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc 3720 gcggacaagc cgcgccgtcg ccactcgacc gccggcgctg aggtctgcct cgtgaagaag 3780 gtgttgctga ctcataccag ggcaatggca acaacgttgc gcaaactatt aactggcgaa 3840 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 3900 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 3960 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 4020 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 4080 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 4140 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 4200 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 4260 cccttaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa 4320 aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg 4380 aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct 4440 taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag 4500 tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4560 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc 4620 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg 4680 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt 4740 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct 4800 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt 4860 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg 4920 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta 4980 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca 5040 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc 5100 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc 5160 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt 5220 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct 5280 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat 5340 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 5400 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgagatcta 5460 tcgatgcatg ccatggtacc cgggagctcg aattcgaagc ttttgccatt ctcaccggat 5520 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 5580 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 5640 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 5700 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 5760 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac 5820 ggcggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc 5880 ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct 5940 acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc 6000 aggcctggta tgagtcagca acaccttctt cacgaggcag acctcagcgc caggcgcacg 6060 aggccggcgc gcggacccgc gccacggccc acgagcggac gccgcagcag gagcgccaga 6120 aggccgccag agaggccgag cgcggccgtg aggcttggac gctagggcag ggcatgaaaa 6180 agcccgtagc gggctgctac gggcgtctga cgcggtggaa agggggaggg gatgttgtct 6240 acatggctct gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc aatcgtcacc 6300 ctttctcggt ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat 6360 caccgcgagt ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc 6420 ggcccgcaac agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc 6480 gctgtcgccg gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag 6540 cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc 6600 ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg 6660 gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc 6720 gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc 6780 gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa 6840 ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag 6900 ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat 6960 aaacataata tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag 7020 cggaggctgg tccggaggcc agacgtgaaa cccaacatac ccctgatcgt aattctgagc 7080 actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg 7140 cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat 7200 gcgttggtgc aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg 7260 cggccaatct tgctcgtctc gctggccggc gccactgtcg actacgccat catggcgaca 7320 gcgcctttcc tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact 7380 ggggcggtag ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac 7440 ttcggcttca tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg 7500 ctgatgggcg gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc 7560 aatttcctga cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta 7620 cgccgggagg ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc 7680 gccgccctga tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt 7740 tgggtcattt tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt 7800 gccgcatttg gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc 7860 cggctcggcg aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc 7920 ctgcttgcct tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg 7980 ggtggcatcg gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt 8040 caggggcagc tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc 8100 ctcctcttca cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt 8160 gcaggcgctg ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc 8220 gcagggcaac gagccgatcg ctgatcgtgg aaacgatagg gacgtcatat ggatctcgcc 8280 tgtcccctca gttcagtaat ttcctgcatt tgcctgtttc cagtcggtag atattccaca 8340 aaacagcagg gaagcagcgc ttttccgctg cataaccctg cttcggggtc attatagcga 8400 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg 8460 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac 8520 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga 8580 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac 8620 // ID AB043475; SV 1; circular; other DNA; STD; SYN; 8620 BP. XX AC AB043475; XX DT 30-MAY-2000 (Rel. 63, Created) DT 28-DEC-2006 (Rel. 90, Last updated, Version 4) XX DE Shuttle vector pNIT6011 DNA, complete sequence. XX KW . XX OS Shuttle vector pNIT6011 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-8620 RA Itoh Y., Nishijyo T.; RT ; RL Submitted (22-MAY-2000) to the INSDC. RL Yoshifumi Itoh, National Food Research Institute, Division of Applied RL Microbiology; kannondai 2-1-2, Tsukuba, Ibaraki 305-8642, Japan RL (E-mail:yosifumi@nfri.affrc.go.jp, Tel:81-298-38-8075, Fax:81-298-38-7996) XX RN [2] RX DOI; 10.1094/MPMI.2000.13.2.232. RX PUBMED; 10659714. RA Heeb S., Itoh Y., Nishijyo T., Schnider U., Keel C., Wade J., Walsh U., RA OGara F., Haas D.; RT "Small, stable shuttle vectors based on the minimal pVS1 replicon for use RT in gram-negative, plant-associated bacteria"; RL Mol. Plant Microbe Interact. 13(2):232-237(2000). XX DR MD5; d22c329a51c97c84431d48e441899d6c. XX FH Key Location/Qualifiers FH FT source 1..8620 FT /organism="Shuttle vector pNIT6011" FT /lab_host="Escherichia coli, Pseudomonas aeruginosa" FT /mol_type="other DNA" FT /db_xref="taxon:126708" FT CDS 195..881 FT /codon_start=1 FT /transl_table=11 FT /product="pVS1 resolvase" FT /function="unknown" FT /note="not required for stable maintenance" FT /note="orf1" FT /protein_id="BAA96337.1" FT /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT FT RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT FT TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI FT DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE FT RQEEQA" FT CDS 878..1093 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="not required for stable maintenance" FT /note="orf2" FT /protein_id="BAA96338.1" FT /translation="MKPHQDGQDEPFFITEEIEAEMIAAGYVFEPPAHVSTVRLHEILA FT GLSDAKLAAWPASLAAEETERRRLKR" FT CDS 1180..1809 FT /codon_start=1 FT /transl_table=11 FT /gene="staA" FT /product="pVS1 partitioning protein StaA" FT /note="similar to IncC2" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="BAA96339.1" FT /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR FT DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP FT VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI FT LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" FT CDS 1830..2045 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="not required for stable mainenance" FT /note="orf3" FT /protein_id="BAA96340.1" FT /translation="MSKSTNTLSAGRPSARSSKAATLASLADTPAMKRVNFQLPAEDHT FT KLKMYAVRQGKTITELLSEYIAQLPE" FT misc_feature 2076..2088 FT /note="KorB box" FT CDS 2238..3311 FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="pVS1 replication protein RepA" FT /note="low copy mutant RepA" FT /protein_id="BAA96341.1" FT /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW FT QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS FT KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP FT GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE FT ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPNVLTVHPDYFLIDPGIGRFLYR FT LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI FT LVMRYRNLIEGEASAGS" FT misc_difference 3015 FT /replace="g" FT /note="present in pME290, pME6010, pNIC6010 and pNIT6010" FT /note="results in increased copy number" FT rep_origin 3377..3571 FT /note="pVS1 origin of replication" FT misc_feature 3377..3384 FT /note="DnaA box" FT repeat_region 3388..3471 FT /rpt_type=DIRECT FT /note="21 bp" FT misc_feature 3472..3563 FT /note="AT-rich region" FT misc_feature 3564..3571 FT /note="DnaA box" FT rep_origin 4264..5093 FT /note="p15A origin of replication" FT misc_feature 5068..5089 FT /note="p15A origin of transfer" FT misc_feature 5450..5502 FT /note="multiple cloning site" FT CDS complement(6289..6966) FT /codon_start=1 FT /transl_table=11 FT /gene="tetR" FT /product="RK2 tetracycline repressor protein TetR" FT /protein_id="BAA96342.1" FT /translation="MFISDKVSSMTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGV FT QQPALYWHFRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRD FT GARIHAGTRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSD FT AGERGGTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRK FT GDD" FT CDS 6970..8244 FT /codon_start=1 FT /transl_table=11 FT /gene="tetA" FT /product="RK2 tetracycline resistance protein TetA" FT /protein_id="BAA96343.1" FT /translation="MSTNLSVIKNPRVQSDQRRLVRRPDVKPNIPLIVILSTVALDAVG FT IGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALVQFACAPVLGALSDRFGRRPILLVS FT LAGATVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSA FT CFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREAL FT NPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFG FT ILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGI FT GMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAG FT AALYLLCLPALRRGLWSGAGQRADR" FT misc_feature 8247..8620 FT /note="RP4 mob region" XX SQ Sequence 8620 BP; 1856 A; 2470 C; 2512 G; 1782 T; 0 other; agatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt 60 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt 120 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc 180 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca 240 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca 300 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc 360 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca 420 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta 480 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt 540 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg 600 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga 660 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac 720 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc 780 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc 840 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca 900 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt 960 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc 1020 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg 1080 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat 1140 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt 1200 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa 1260 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg 1320 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac 1380 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg 1440 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca 1500 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag 1560 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc 1620 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt 1680 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca 1740 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa 1800 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg 1860 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag 1920 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg 1980 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag 2040 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg 2100 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc 2160 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc 2220 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg 2280 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca 2340 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag 2400 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg 2460 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca 2520 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc 2580 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg 2640 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat 2700 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac 2760 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca 2820 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc 2880 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa 2940 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag 3000 aaggcaagaa cccgaacgtg ctgacggttc accccgatta ctttttgatc gatcccggca 3060 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt 3120 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca 3180 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg 3240 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg 3300 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc 3360 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga 3420 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag 3480 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta 3540 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc 3600 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc 3660 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc 3720 gcggacaagc cgcgccgtcg ccactcgacc gccggcgctg aggtctgcct cgtgaagaag 3780 gtgttgctga ctcataccag ggcaatggca acaacgttgc gcaaactatt aactggcgaa 3840 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 3900 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 3960 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 4020 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 4080 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 4140 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 4200 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 4260 cccttaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa 4320 aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg 4380 aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct 4440 taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag 4500 tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4560 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc 4620 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg 4680 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt 4740 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct 4800 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt 4860 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg 4920 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta 4980 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca 5040 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc 5100 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc 5160 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt 5220 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct 5280 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat 5340 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 5400 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgagatcta 5460 tcgatgcatg ccatggtacc cgggagctcg aattcgaagc ttttgccatt ctcaccggat 5520 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 5580 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 5640 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 5700 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 5760 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac 5820 ggcggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc 5880 ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct 5940 acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc 6000 aggcctggta tgagtcagca acaccttctt cacgaggcag acctcagcgc caggcgcacg 6060 aggccggcgc gcggacccgc gccacggccc acgagcggac gccgcagcag gagcgccaga 6120 aggccgccag agaggccgag cgcggccgtg aggcttggac gctagggcag ggcatgaaaa 6180 agcccgtagc gggctgctac gggcgtctga cgcggtggaa agggggaggg gatgttgtct 6240 acatggctct gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc aatcgtcacc 6300 ctttctcggt ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat 6360 caccgcgagt ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc 6420 ggcccgcaac agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc 6480 gctgtcgccg gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag 6540 cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc 6600 ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg 6660 gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc 6720 gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc 6780 gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa 6840 ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag 6900 ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat 6960 aaacataata tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag 7020 cggaggctgg tccggaggcc agacgtgaaa cccaacatac ccctgatcgt aattctgagc 7080 actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg 7140 cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat 7200 gcgttggtgc aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg 7260 cggccaatct tgctcgtctc gctggccggc gccactgtcg actacgccat catggcgaca 7320 gcgcctttcc tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact 7380 ggggcggtag ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac 7440 ttcggcttca tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg 7500 ctgatgggcg gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc 7560 aatttcctga cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta 7620 cgccgggagg ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc 7680 gccgccctga tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt 7740 tgggtcattt tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt 7800 gccgcatttg gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc 7860 cggctcggcg aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc 7920 ctgcttgcct tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg 7980 ggtggcatcg gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt 8040 caggggcagc tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc 8100 ctcctcttca cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt 8160 gcaggcgctg ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc 8220 gcagggcaac gagccgatcg ctgatcgtgg aaacgatagg gacgtcatat ggatctcgcc 8280 tgtcccctca gttcagtaat ttcctgcatt tgcctgtttc cagtcggtag atattccaca 8340 aaacagcagg gaagcagcgc ttttccgctg cataaccctg cttcggggtc attatagcga 8400 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg 8460 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac 8520 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga 8580 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac 8620 // ID AB043476; SV 1; circular; other DNA; STD; SYN; 8620 BP. XX AC AB043476; XX DT 30-MAY-2000 (Rel. 63, Created) DT 28-DEC-2006 (Rel. 90, Last updated, Version 4) XX DE Shuttle vector pNIT6012 DNA, complete sequence. XX KW . XX OS Shuttle vector pNIT6012 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-8620 RA Itoh Y., Nishijyo T.; RT ; RL Submitted (22-MAY-2000) to the INSDC. RL Yoshifumi Itoh, National Food Research Institute, Division of Applied RL Microbiology; kannondai 2-1-2, Tsukuba, Ibaraki 305-8642, Japan RL (E-mail:yosifumi@nfri.affrc.go.jp, Tel:81-298-38-8075, Fax:81-298-38-7996) XX RN [2] RX DOI; 10.1094/MPMI.2000.13.2.232. RX PUBMED; 10659714. RA Heeb S., Itoh Y., Nishijyo T., Schnider U., Keel C., Wade J., Walsh U., RA OGara F., Haas D.; RT "Small, stable shuttle vectors based on the minimal pVS1 replicon for use RT in gram-negative, plant-associated bacteria"; RL Mol. Plant Microbe Interact. 13(2):232-237(2000). XX DR MD5; 737df5d1a3896c9f85fce063fce7be98. DR EuropePMC; PMC3416211; 22685277. XX FH Key Location/Qualifiers FH FT source 1..8620 FT /organism="Shuttle vector pNIT6012" FT /lab_host="Escherichia coli, Pseudomonas aeruginosa" FT /mol_type="other DNA" FT /db_xref="taxon:126709" FT CDS 195..881 FT /codon_start=1 FT /transl_table=11 FT /product="pVS1 resolvase" FT /function="unknown" FT /note="not required for stable maintenance" FT /note="orf1" FT /protein_id="BAA96344.1" FT /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT FT RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT FT TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI FT DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE FT RQEEQA" FT CDS 878..1093 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="not required for stable maintenance" FT /note="orf2" FT /protein_id="BAA96345.1" FT /translation="MKPHQDGQDEPFFITEEIEAEMIAAGYVFEPPAHVSTVRLHEILA FT GLSDAKLAAWPASLAAEETERRRLKR" FT CDS 1180..1809 FT /codon_start=1 FT /transl_table=11 FT /gene="staA" FT /product="pVS1 partitioning protein StaA" FT /note="similar to IncC2" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="BAA96346.1" FT /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR FT DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP FT VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI FT LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" FT CDS 1830..2045 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="not required for stable mainenance" FT /note="orf3" FT /protein_id="BAA96347.1" FT /translation="MSKSTNTLSAGRPSARSSKAATLASLADTPAMKRVNFQLPAEDHT FT KLKMYAVRQGKTITELLSEYIAQLPE" FT misc_feature 2076..2088 FT /note="KorB box" FT CDS 2238..3311 FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="pVS1 replication protein RepA" FT /function="pVS1 replication protein" FT /note="high copy mutant RepA" FT /protein_id="BAA96348.1" FT /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW FT QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS FT KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP FT GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE FT ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR FT LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI FT LVMRYRNLIEGEASAGS" FT misc_difference 2974 FT /replace="c" FT /note="present in pME290, pME6010, pNIC6010 and pNIT6010" FT /note="results in decreased copy number" FT rep_origin 3377..3571 FT /note="pVS1 origin of replication" FT misc_feature 3377..3384 FT /note="DnaA box" FT repeat_region 3388..3471 FT /rpt_type=DIRECT FT /note="21 bp" FT misc_feature 3472..3563 FT /note="AT-rich region" FT misc_feature 3564..3571 FT /note="DnaA box" FT rep_origin 4264..5093 FT /note="p15A origin of replication" FT misc_feature 5068..5089 FT /note="p15A origin of transfer" FT misc_feature 5450..5502 FT /note="multiple cloning site" FT CDS complement(6289..6966) FT /codon_start=1 FT /transl_table=11 FT /gene="tetR" FT /product="RK2 tetracycline repressor protein TetR" FT /protein_id="BAA96349.1" FT /translation="MFISDKVSSMTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGV FT QQPALYWHFRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRD FT GARIHAGTRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSD FT AGERGGTVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRK FT GDD" FT CDS 6970..8244 FT /codon_start=1 FT /transl_table=11 FT /gene="tetA" FT /product="RK2 tetracycline resistance protein TetA" FT /protein_id="BAA96350.1" FT /translation="MSTNLSVIKNPRVQSDQRRLVRRPDVKPNIPLIVILSTVALDAVG FT IGLIMPVLPGLLRDLVHSNDVTAHYGILLALYALVQFACAPVLGALSDRFGRRPILLVS FT LAGATVDYAIMATAPFLWVLYIGRIVAGITGATGAVAGAYIADITDGDERARHFGFMSA FT CFGFGMVAGPVLGGLMGGFSPHAPFFAAAALNGLNFLTGCFLLPESHKGERRPLRREAL FT NPLASFRWARGMTVVAALMAVFFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFG FT ILHSLAQAMITGPVAARLGERRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGI FT GMPALQAMLSRQVDEERQGQLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAG FT AALYLLCLPALRRGLWSGAGQRADR" FT misc_feature 8247..8620 FT /note="RP4 mob region" XX SQ Sequence 8620 BP; 1855 A; 2469 C; 2513 G; 1783 T; 0 other; agatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt 60 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt 120 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc 180 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca 240 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca 300 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc 360 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca 420 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta 480 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt 540 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg 600 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga 660 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac 720 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc 780 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc 840 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca 900 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt 960 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc 1020 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg 1080 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat 1140 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt 1200 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa 1260 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg 1320 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac 1380 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg 1440 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca 1500 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag 1560 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc 1620 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt 1680 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca 1740 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa 1800 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg 1860 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag 1920 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg 1980 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag 2040 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg 2100 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc 2160 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc 2220 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg 2280 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca 2340 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag 2400 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg 2460 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca 2520 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc 2580 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg 2640 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat 2700 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac 2760 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca 2820 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc 2880 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa 2940 ccgggcggcc ggagtacatc gagatcgagc tagttgattg gatgtaccgc gagatcacag 3000 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca 3060 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt 3120 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca 3180 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg 3240 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg 3300 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc 3360 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga 3420 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag 3480 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta 3540 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc 3600 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc 3660 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc 3720 gcggacaagc cgcgccgtcg ccactcgacc gccggcgctg aggtctgcct cgtgaagaag 3780 gtgttgctga ctcataccag ggcaatggca acaacgttgc gcaaactatt aactggcgaa 3840 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 3900 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 3960 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 4020 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 4080 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 4140 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 4200 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 4260 cccttaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa 4320 aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg 4380 aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct 4440 taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag 4500 tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4560 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc 4620 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg 4680 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt 4740 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct 4800 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt 4860 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg 4920 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta 4980 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca 5040 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc 5100 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc 5160 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt 5220 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct 5280 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat 5340 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 5400 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgagatcta 5460 tcgatgcatg ccatggtacc cgggagctcg aattcgaagc ttttgccatt ctcaccggat 5520 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta 5580 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc 5640 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat 5700 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc 5760 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac 5820 ggcggctttg ttgaataaat cgaacttttg ctgagttgaa ggatcagatc acgcatcttc 5880 ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa aatcaccaac tggtccacct 5940 acaacaaagc tctcatcaac cgtggctccc tcactttctg gctggatgat ggggcgattc 6000 aggcctggta tgagtcagca acaccttctt cacgaggcag acctcagcgc caggcgcacg 6060 aggccggcgc gcggacccgc gccacggccc acgagcggac gccgcagcag gagcgccaga 6120 aggccgccag agaggccgag cgcggccgtg aggcttggac gctagggcag ggcatgaaaa 6180 agcccgtagc gggctgctac gggcgtctga cgcggtggaa agggggaggg gatgttgtct 6240 acatggctct gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc aatcgtcacc 6300 ctttctcggt ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat 6360 caccgcgagt ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc 6420 ggcccgcaac agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc 6480 gctgtcgccg gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag 6540 cgcattgacg gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc 6600 ggccgtttcc atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg 6660 gtaggcgagc agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc 6720 gtcgtcggct ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc 6780 gtcgagcagc gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa 6840 ccgttccgcc agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag 6900 ggcggcacgg atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat 6960 aaacataata tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag 7020 cggaggctgg tccggaggcc agacgtgaaa cccaacatac ccctgatcgt aattctgagc 7080 actgtcgcgc tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg 7140 cgcgatctgg ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat 7200 gcgttggtgc aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg 7260 cggccaatct tgctcgtctc gctggccggc gccactgtcg actacgccat catggcgaca 7320 gcgcctttcc tttgggttct ctatatcggg cggatcgtgg ccggcatcac cggggcgact 7380 ggggcggtag ccggcgctta tattgccgat atcactgatg gcgatgagcg cgcgcggcac 7440 ttcggcttca tgagcgcctg tttcgggttc gggatggtcg cgggacctgt gctcggtggg 7500 ctgatgggcg gtttctcccc ccacgctccg ttcttcgccg cggcagcctt gaacggcctc 7560 aatttcctga cgggctgttt ccttttgccg gagtcgcaca aaggcgaacg ccggccgtta 7620 cgccgggagg ctctcaaccc gctcgcttcg ttccggtggg cccggggcat gaccgtcgtc 7680 gccgccctga tggcggtctt cttcatcatg caacttgtcg gacaggtgcc ggccgcgctt 7740 tgggtcattt tcggcgagga tcgctttcac tgggacgcga ccacgatcgg catttcgctt 7800 gccgcatttg gcattctgca ttcactcgcc caggcaatga tcaccggccc tgtagccgcc 7860 cggctcggcg aaaggcgggc actcatgctc ggaatgattg ccgacggcac aggctacatc 7920 ctgcttgcct tcgcgacacg gggatggatg gcgttcccga tcatggtcct gcttgcttcg 7980 ggtggcatcg gaatgccggc gctgcaagca atgttgtcca ggcaggtgga tgaggaacgt 8040 caggggcagc tgcaaggctc actggcggcg ctcaccagcc tgacctcgat cgtcggaccc 8100 ctcctcttca cggcgatcta tgcggcttct ataacaacgt ggaacgggtg ggcatggatt 8160 gcaggcgctg ccctctactt gctctgcctg ccggcgctgc gtcgcgggct ttggagcggc 8220 gcagggcaac gagccgatcg ctgatcgtgg aaacgatagg gacgtcatat ggatctcgcc 8280 tgtcccctca gttcagtaat ttcctgcatt tgcctgtttc cagtcggtag atattccaca 8340 aaacagcagg gaagcagcgc ttttccgctg cataaccctg cttcggggtc attatagcga 8400 ttttttcggt atatccatcc tttttcgcac gatatacagg attttgccaa agggttcgtg 8460 tagactttcc ttggtgtatc caacggcgtc agccgggcag gataggtgaa gtaggcccac 8520 ccgcgagcgg gtgttccttc ttcactgtcc cttattcgca cctggcggtg ctcaacggga 8580 atcctgctct gcgaggctgg ccggctaccg ccggcgtaac 8620 // ID AB043580; SV 1; circular; other DNA; STD; SYN; 8253 BP. XX AC AB043580; XX DT 30-MAY-2000 (Rel. 63, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 3) XX DE Shuttle vector pNIC6010 DNA, complete sequence. XX KW . XX OS Shuttle vector pNIC6010 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-8253 RA Itoh Y., Nishijyo T.; RT ; RL Submitted (23-MAY-2000) to the INSDC. RL Yoshifumi Itoh, National Food Research Institute, Division of Applied RL Microbiology; kannondai 2-1-2, Tsukuba, Ibaraki 305-8642, Japan RL (E-mail:yosifumi@nfri.affrc.go.jp, Tel:81-298-38-8075, Fax:81-298-38-7997) XX RN [2] RX DOI; 10.1094/MPMI.2000.13.2.232. RX PUBMED; 10659714. RA Heeb S., Itoh Y., Nishijyo T., Schnider U., Keel C., Wade J., Walsh U., RA OGara F., Haas D.; RT "Small, stable shuttle vectors based on the minimal pVS1 replicon for use RT in gram-negative, plant-associated bacteria"; RL Mol. Plant Microbe Interact. 13(2):232-237(2000). XX DR MD5; 2a7abfc93548ea77e92a0c2001eb6051. XX FH Key Location/Qualifiers FH FT source 1..8253 FT /organism="Shuttle vector pNIC6010" FT /lab_host="Escherichia coli, Pseudomonas aeruginosa" FT /mol_type="other DNA" FT /db_xref="taxon:126840" FT CDS 195..881 FT /codon_start=1 FT /transl_table=11 FT /product="pVS1 resolvase" FT /note="not required for stable maintenance" FT /note="orf1" FT /protein_id="BAA96311.1" FT /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT FT RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT FT TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI FT DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE FT RQEEQA" FT CDS 878..1093 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /note="not required for stable maintenance" FT /note="orf2" FT /protein_id="BAA96312.1" FT /translation="MKPHQDGQDEPFFITEEIEAEMIAAGYVFEPPAHVSTVRLHEILA FT GLSDAKLAAWPASLAAEETERRRLKR" FT CDS 1180..1809 FT /codon_start=1 FT /transl_table=11 FT /gene="staA" FT /product="pVS1 partitioning protein StaA" FT /note="similar to IncC2" FT /note="stable maintenance of pVS1 replicon" FT /protein_id="BAA96313.1" FT /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR FT DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP FT VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI FT LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" FT CDS 1830..2045 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="not required for stable maintenance" FT /note="orf3" FT /protein_id="BAA96314.1" FT /translation="MSKSTNTLSAGRPSARSSKAATLASLADTPAMKRVNFQLPAEDHT FT KLKMYAVRQGKTITELLSEYIAQLPE" FT misc_feature 2076..2088 FT /note="KroB box" FT CDS 2238..3311 FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="pVS1 replication protein RepA" FT /protein_id="BAA96315.1" FT /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW FT QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS FT KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP FT GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE FT ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR FT LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI FT LVMRYRNLIEGEASAGS" FT misc_difference 2974 FT /replace="t" FT /note="resent in pME294, pME6012, pNIC6012 and pNIT6012" FT /note="results in increased copy number" FT /experiment="experimental evidence, no additional details FT recorded" FT misc_difference 3015 FT /replace="a" FT /note="present in pME292, pME6011, pNIC6011 and pNIT6011" FT /note="results in decreased copy number" FT /experiment="experimental evidence, no additional details FT recorded" FT rep_origin 3377..3571 FT /note="pVS1 origin of replication" FT misc_feature 3377..3384 FT /note="DnaA box" FT repeat_region 3388..3471 FT /rpt_type=DIRECT FT /note="21 bp repeats" FT misc_feature 3472..3563 FT /note="AT-rich region" FT misc_feature 3564..3571 FT /note="DnaA box" FT rep_origin 4264..5093 FT /note="p15A origin of replication" FT misc_feature 5068..5089 FT /note="p15A origin of transfer" FT misc_feature 5450..5502 FT /note="multi cloning site" FT CDS complement(6670..7530) FT /codon_start=1 FT /transl_table=11 FT /gene="bla" FT /product="beta lactamase" FT /function="ampicillin/carbenycillin resistance" FT /note="derived from Tn801" FT /protein_id="BAA96316.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDKLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT misc_feature 7907..8253 FT /note="mob region from RP4" XX SQ Sequence 8253 BP; 1991 A; 2241 C; 2223 G; 1798 T; 0 other; agatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt 60 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt 120 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc 180 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca 240 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca 300 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc 360 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca 420 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta 480 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt 540 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg 600 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga 660 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac 720 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc 780 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc 840 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca 900 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt 960 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc 1020 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg 1080 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat 1140 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt 1200 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa 1260 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg 1320 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac 1380 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg 1440 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca 1500 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag 1560 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc 1620 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt 1680 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca 1740 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa 1800 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg 1860 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag 1920 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg 1980 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag 2040 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg 2100 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc 2160 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc 2220 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg 2280 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca 2340 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag 2400 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg 2460 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca 2520 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc 2580 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg 2640 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat 2700 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac 2760 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca 2820 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc 2880 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa 2940 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag 3000 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca 3060 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt 3120 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca 3180 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg 3240 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg 3300 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc 3360 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga 3420 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag 3480 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta 3540 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc 3600 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc 3660 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc 3720 gcggacaagc cgcgccgtcg ccactcgacc gccggcgctg aggtctgcct cgtgaagaag 3780 gtgttgctga ctcataccag ggcaatggca acaacgttgc gcaaactatt aactggcgaa 3840 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 3900 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 3960 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 4020 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 4080 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 4140 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 4200 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 4260 cccttaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa 4320 aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg 4380 aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct 4440 taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag 4500 tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4560 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc 4620 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg 4680 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt 4740 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct 4800 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt 4860 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg 4920 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta 4980 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca 5040 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc 5100 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc 5160 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt 5220 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct 5280 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat 5340 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 5400 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgagatcta 5460 tcgatgcatg ccatggtacc cgggagctcg aattcgaagc ttatgcattt ctttccagac 5520 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt 5580 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt 5640 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc 5700 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt 5760 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa 5820 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt 5880 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc 5940 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt 6000 ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct 6060 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg 6120 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt 6180 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa 6240 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc 6300 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc 6360 ttcacgaggc agacctcagc gcccgcgagc gagccgccga gctggccggc cagctcaccg 6420 cacaccaggg acaggcggcc gcagttgctg gcacgcctgg caccggccga aaccaaaaca 6480 cccagggcga agaagtaggg cggtggattg gtctggcggt agtggggtct gacgctcagt 6540 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6600 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactg 6660 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 6720 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 6780 atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc cagatttatc 6840 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 6900 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 6960 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat 7020 ggcttcattc agctccggtt cccaacgatc aaggcgggtt acatgatccc ccatgttgtg 7080 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 7140 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 7200 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 7260 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcaccacata gcagaacttt 7320 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 7380 gttgagatcc agctcgatgt aacccactcg tgcacccaac ttatcttcag catcttttac 7440 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 7500 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 7560 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 7620 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 7680 tatcatgaca ttaacctata aaaataagcg tatcacgagg ccctttcgtc ttcaagaatt 7740 ttataaaccg tggagcgggc aatactgagc tgatgagcaa tttccgttgc accagtgccc 7800 ttctgatgaa gcgtcagcac gacgttcctg tccacggtac gcctgcggcc aaatttgatt 7860 cctttcagct ttgcttcctg tcggccctca ttcgtgcgct ctaggatctc gcctgtcccc 7920 tcagttcagt aatttcctgc atttgcctgt ttccagtcgg tagatattcc acaaaacagc 7980 agggaagcag cgcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc 8040 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt 8100 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag 8160 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc 8220 tctgcgaggc tggccggcta ccgccggcgt aac 8253 // ID AB043581; SV 1; circular; other DNA; STD; SYN; 8253 BP. XX AC AB043581; XX DT 30-MAY-2000 (Rel. 63, Created) DT 28-DEC-2006 (Rel. 90, Last updated, Version 4) XX DE Shuttle vector pNIC6011 DNA, complete sequence. XX KW . XX OS Shuttle vector pNIC6011 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-8253 RA Itoh Y., Nishijyo T.; RT ; RL Submitted (23-MAY-2000) to the INSDC. RL Yoshifumi Itoh, National Food Research Institute, Division of Applied RL Microbiology; kannondai 2-1-2, Tsukuba, Ibaraki 305-8642, Japan RL (E-mail:yosifumi@nfri.affrc.go.jp, Tel:81-298-38-8075, Fax:81-298-38-7997) XX RN [2] RX DOI; 10.1094/MPMI.2000.13.2.232. RX PUBMED; 10659714. RA Heeb S., Itoh Y., Nishijyo T., Schnider U., Keel C., Wade J., Walsh U., RA OGara F., Haas D.; RT "Small, stable shuttle vectors based on the minimal pVS1 replicon for use RT in gram-negative, plant-associated bacteria"; RL Mol. Plant Microbe Interact. 13(2):232-237(2000). XX DR MD5; 72c43e322f5fbe8734ba377c032f17b1. DR EuropePMC; PMC95480; 11673419. XX FH Key Location/Qualifiers FH FT source 1..8253 FT /organism="Shuttle vector pNIC6011" FT /lab_host="Escherichia coli, Pseudomonas aeruginosa" FT /mol_type="other DNA" FT /db_xref="taxon:126841" FT CDS 195..881 FT /codon_start=1 FT /transl_table=11 FT /product="pVS1 resolvase" FT /note="not required for stable maintenance" FT /note="orf1" FT /protein_id="BAA96317.1" FT /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT FT RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT FT TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI FT DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE FT RQEEQA" FT CDS 878..1093 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /note="not required for stable maintenance" FT /note="orf2" FT /protein_id="BAA96318.1" FT /translation="MKPHQDGQDEPFFITEEIEAEMIAAGYVFEPPAHVSTVRLHEILA FT GLSDAKLAAWPASLAAEETERRRLKR" FT CDS 1180..1809 FT /codon_start=1 FT /transl_table=11 FT /gene="staA" FT /product="pVS1 partitioning protein StaA" FT /note="similar to IncC2" FT /note="stable maintenance of pVS1 replicon" FT /protein_id="BAA96319.1" FT /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR FT DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP FT VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI FT LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" FT CDS 1830..2045 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="not required for stable maintenance" FT /note="orf3" FT /protein_id="BAA96320.1" FT /translation="MSKSTNTLSAGRPSARSSKAATLASLADTPAMKRVNFQLPAEDHT FT KLKMYAVRQGKTITELLSEYIAQLPE" FT misc_feature 2076..2088 FT /note="KroB box" FT CDS 2238..3311 FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="pVS1 replication protein RepA" FT /note="low copy mutant RepA" FT /protein_id="BAA96321.1" FT /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW FT QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS FT KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP FT GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE FT ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPNVLTVHPDYFLIDPGIGRFLYR FT LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI FT LVMRYRNLIEGEASAGS" FT misc_difference 3015 FT /replace="g" FT /note="present in pME290, pME6010, pNIC6010 and pNIT6010" FT /note="results in increased copy number" FT /experiment="experimental evidence, no additional details FT recorded" FT rep_origin 3377..3571 FT /note="pVS1 origin of replication" FT misc_feature 3377..3384 FT /note="DnaA box" FT repeat_region 3388..3471 FT /rpt_type=DIRECT FT /note="21 bp repeats" FT misc_feature 3472..3563 FT /note="AT-rich region" FT misc_feature 3564..3571 FT /note="DnaA box" FT rep_origin 4264..5093 FT /note="p15A origin of replication" FT misc_feature 5068..5089 FT /note="p15A origin of transfer" FT misc_feature 5450..5502 FT /note="multi cloning site" FT CDS complement(6670..7530) FT /codon_start=1 FT /transl_table=11 FT /gene="bla" FT /product="beta lactamase" FT /function="ampicillin/carbenycillin resistance" FT /note="derived from Tn801" FT /protein_id="BAA96322.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDKLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT misc_feature 7907..8253 FT /note="mob region from RP4" XX SQ Sequence 8253 BP; 1992 A; 2241 C; 2222 G; 1798 T; 0 other; agatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt 60 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt 120 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc 180 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca 240 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca 300 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc 360 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca 420 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta 480 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt 540 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg 600 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga 660 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac 720 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc 780 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc 840 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca 900 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt 960 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc 1020 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg 1080 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat 1140 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt 1200 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa 1260 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg 1320 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac 1380 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg 1440 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca 1500 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag 1560 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc 1620 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt 1680 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca 1740 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa 1800 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg 1860 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag 1920 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg 1980 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag 2040 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg 2100 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc 2160 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc 2220 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg 2280 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca 2340 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag 2400 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg 2460 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca 2520 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc 2580 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg 2640 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat 2700 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac 2760 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca 2820 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc 2880 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa 2940 ccgggcggcc ggagtacatc gagatcgagc tagctgattg gatgtaccgc gagatcacag 3000 aaggcaagaa cccgaacgtg ctgacggttc accccgatta ctttttgatc gatcccggca 3060 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt 3120 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca 3180 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg 3240 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg 3300 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc 3360 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga 3420 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag 3480 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta 3540 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc 3600 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc 3660 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc 3720 gcggacaagc cgcgccgtcg ccactcgacc gccggcgctg aggtctgcct cgtgaagaag 3780 gtgttgctga ctcataccag ggcaatggca acaacgttgc gcaaactatt aactggcgaa 3840 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 3900 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 3960 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 4020 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 4080 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 4140 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 4200 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 4260 cccttaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa 4320 aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg 4380 aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct 4440 taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag 4500 tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4560 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc 4620 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg 4680 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt 4740 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct 4800 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt 4860 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg 4920 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta 4980 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca 5040 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc 5100 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc 5160 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt 5220 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct 5280 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat 5340 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 5400 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgagatcta 5460 tcgatgcatg ccatggtacc cgggagctcg aattcgaagc ttatgcattt ctttccagac 5520 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt 5580 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt 5640 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc 5700 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt 5760 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa 5820 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt 5880 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc 5940 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt 6000 ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct 6060 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg 6120 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt 6180 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa 6240 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc 6300 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc 6360 ttcacgaggc agacctcagc gcccgcgagc gagccgccga gctggccggc cagctcaccg 6420 cacaccaggg acaggcggcc gcagttgctg gcacgcctgg caccggccga aaccaaaaca 6480 cccagggcga agaagtaggg cggtggattg gtctggcggt agtggggtct gacgctcagt 6540 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6600 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactg 6660 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 6720 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 6780 atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc cagatttatc 6840 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 6900 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 6960 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat 7020 ggcttcattc agctccggtt cccaacgatc aaggcgggtt acatgatccc ccatgttgtg 7080 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 7140 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 7200 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 7260 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcaccacata gcagaacttt 7320 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 7380 gttgagatcc agctcgatgt aacccactcg tgcacccaac ttatcttcag catcttttac 7440 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 7500 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 7560 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 7620 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 7680 tatcatgaca ttaacctata aaaataagcg tatcacgagg ccctttcgtc ttcaagaatt 7740 ttataaaccg tggagcgggc aatactgagc tgatgagcaa tttccgttgc accagtgccc 7800 ttctgatgaa gcgtcagcac gacgttcctg tccacggtac gcctgcggcc aaatttgatt 7860 cctttcagct ttgcttcctg tcggccctca ttcgtgcgct ctaggatctc gcctgtcccc 7920 tcagttcagt aatttcctgc atttgcctgt ttccagtcgg tagatattcc acaaaacagc 7980 agggaagcag cgcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc 8040 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt 8100 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag 8160 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc 8220 tctgcgaggc tggccggcta ccgccggcgt aac 8253 // ID AB043582; SV 1; circular; other DNA; STD; SYN; 8253 BP. XX AC AB043582; XX DT 30-MAY-2000 (Rel. 63, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 3) XX DE Shuttle vector pNIC6012 DNA, complete sequence. XX KW . XX OS Shuttle vector pNIC6012 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-8253 RA Itoh Y., Nishijyo T.; RT ; RL Submitted (23-MAY-2000) to the INSDC. RL Yoshifumi Itoh, National Food Research Institute, Division of Applied RL Microbiology; kannondai 2-1-2, Tsukuba, Ibaraki 305-8642, Japan RL (E-mail:yosifumi@nfri.affrc.go.jp, Tel:81-298-38-8075, Fax:81-298-38-7997) XX RN [2] RX DOI; 10.1094/MPMI.2000.13.2.232. RX PUBMED; 10659714. RA Heeb S., Itoh Y., Nishijyo T., Schnider U., Keel C., Wade J., Walsh U., RA OGara F., Haas D.; RT "Small, stable shuttle vectors based on the minimal pVS1 replicon for use RT in gram-negative, plant-associated bacteria"; RL Mol. Plant Microbe Interact. 13(2):232-237(2000). XX DR MD5; f314afe7e23f203d16a86e88e93e1933. XX FH Key Location/Qualifiers FH FT source 1..8253 FT /organism="Shuttle vector pNIC6012" FT /lab_host="Escherichia coli, Pseudomonas aeruginosa" FT /mol_type="other DNA" FT /db_xref="taxon:126842" FT CDS 195..881 FT /codon_start=1 FT /transl_table=11 FT /product="pVS1 resolvase" FT /note="not required for stable maintenance" FT /note="orf1" FT /protein_id="BAA96323.1" FT /translation="MNKSAAAGLLGYARVSTDDQDLTNQRAELHAAGCTKLFSEKITGT FT RRDRPELARMLDHLRPGDVVTVTRLDRLARSTRDLLDIAERIQEAGAGLRSLAEPWADT FT TTPAGRMVLTVFAGIAEFERSLIIDRTRSGREAAKARGVKFGPRPTLTPAQIAHARELI FT DQEGRTVKEAAALLGVHRSTLYRALERSEEVTPTEARRRGAFREDALTEADALAAAENE FT RQEEQA" FT CDS 878..1093 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /note="not required for stable maintenance" FT /note="orf2" FT /protein_id="BAA96324.1" FT /translation="MKPHQDGQDEPFFITEEIEAEMIAAGYVFEPPAHVSTVRLHEILA FT GLSDAKLAAWPASLAAEETERRRLKR" FT CDS 1180..1809 FT /codon_start=1 FT /transl_table=11 FT /gene="staA" FT /product="pVS1 partitioning protein StaA" FT /note="similar to IncC2" FT /note="stable maintenance of pVS1 replicon" FT /protein_id="BAA96325.1" FT /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR FT DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP FT VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI FT LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI" FT CDS 1830..2045 FT /codon_start=1 FT /transl_table=11 FT /product="hypothetical protein" FT /function="unknown" FT /note="not required for stable maintenance" FT /note="orf3" FT /protein_id="BAA96326.1" FT /translation="MSKSTNTLSAGRPSARSSKAATLASLADTPAMKRVNFQLPAEDHT FT KLKMYAVRQGKTITELLSEYIAQLPE" FT misc_feature 2076..2088 FT /note="KroB box" FT CDS 2238..3311 FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="pVS1 replication protein RepA" FT /note="high copy mutant RepA" FT /protein_id="BAA96327.1" FT /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW FT QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS FT KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP FT GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE FT ALISRYKIVKSETGRPEYIEIELVDWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR FT LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI FT LVMRYRNLIEGEASAGS" FT misc_difference 2974 FT /replace="c" FT /note="present in pME290, pME6010, pNIC6010 and pNIT6010" FT /note="results in decreased copy number" FT /experiment="experimental evidence, no additional details FT recorded" FT rep_origin 3377..3571 FT /note="pVS1 origin of replication" FT misc_feature 3377..3384 FT /note="DnaA box" FT repeat_region 3388..3471 FT /rpt_type=DIRECT FT /note="21 bp repeats" FT misc_feature 3472..3563 FT /note="AT-rich region" FT misc_feature 3564..3571 FT /note="DnaA box" FT rep_origin 4264..5093 FT /note="p15A origin of replication" FT misc_feature 5068..5089 FT /note="p15A origin of transfer" FT misc_feature 5450..5502 FT /note="multi cloning site" FT CDS complement(6670..7530) FT /codon_start=1 FT /transl_table=11 FT /gene="bla" FT /product="beta lactamase" FT /function="ampicillin/carbenycillin resistance" FT /note="derived from Tn801" FT /protein_id="BAA96328.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDKLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT misc_feature 7907..8253 FT /note="mob region from RP4" XX SQ Sequence 8253 BP; 1991 A; 2240 C; 2223 G; 1799 T; 0 other; agatccaacc cctccgctgc tatagtgcag tcggcttctg acgttcagtg cagccgtctt 60 ctgaaaacga catgtcgcac aagtcctaag ttacgcgaca ggctgccgcc ctgccctttt 120 cctggcgttt tcttgtcgcg tgttttagtc gcataaagta gaatacttgc gactagaacc 180 ggagacatta cgccatgaac aagagcgccg ccgctggcct gctgggctat gcccgcgtca 240 gcaccgacga ccaggacttg accaaccaac gggccgaact gcacgcggcc ggctgcacca 300 agctgttttc cgagaagatc accggcacca ggcgcgaccg cccggagctg gccaggatgc 360 ttgaccacct acgccctggc gacgttgtga cagtgaccag gctagaccgc ctggcccgca 420 gcacccgcga cctactggac attgccgagc gcatccagga ggccggcgcg ggcctgcgta 480 gcctggcaga gccgtgggcc gacaccacca cgccggccgg ccgcatggtg ttgaccgtgt 540 tcgccggcat tgccgagttc gagcgttccc taatcatcga ccgcacccgg agcgggcgcg 600 aggccgccaa ggcccgaggc gtgaagtttg gcccccgccc taccctcacc ccggcacaga 660 tcgcgcacgc ccgcgagctg atcgaccagg aaggccgcac cgtgaaagag gcggctgcac 720 tgcttggcgt gcatcgctcg accctgtacc gcgcacttga gcgcagcgag gaagtgacgc 780 ccaccgaggc caggcggcgc ggtgccttcc gtgaggacgc attgaccgag gccgacgccc 840 tggcggccgc cgagaatgaa cgccaagagg aacaagcatg aaaccgcacc aggacggcca 900 ggacgaaccg tttttcatta ccgaagagat cgaggcggag atgatcgcgg ccgggtacgt 960 gttcgagccg cccgcgcacg tctcaaccgt gcggctgcat gaaatcctgg ccggtttgtc 1020 tgatgccaag ctggcggcct ggccggccag cttggccgct gaagaaaccg agcgccgccg 1080 tctaaaaagg tgatgtgtat ttgagtaaaa cagcttgcgt catgcggtcg ctgcgtatat 1140 gatgcgatga gtaaataaac aaatacgcaa ggggaacgca tgaaggttat cgctgtactt 1200 aaccagaaag gcgggtcagg caagacgacc atcgcaaccc atctagcccg cgccctgcaa 1260 ctcgccgggg ccgatgttct gttagtcgat tccgatcccc agggcagtgc ccgcgattgg 1320 gcggccgtgc gggaagatca accgctaacc gttgtcggca tcgaccgccc gacgattgac 1380 cgcgacgtga aggccatcgg ccggcgcgac ttcgtagtga tcgacggagc gccccaggcg 1440 gcggacttgg ctgtgtccgc gatcaaggca gccgacttcg tgctgattcc ggtgcagcca 1500 agcccttacg acatatgggc caccgccgac ctggtggagc tggttaagca gcgcattgag 1560 gtcacggatg gaaggctaca agcggccttt gtcgtgtcgc gggcgatcaa aggcacgcgc 1620 atcggcggtg aggttgccga ggcgctggcc gggtacgagc tgcccattct tgagtcccgt 1680 atcacgcagc gcgtgagcta cccaggcact gccgccgccg gcacaaccgt tcttgaatca 1740 gaacccgagg gcgacgctgc ccgcgaggtc caggcgctgg ccgctgaaat taaatcaaaa 1800 ctcatttgag ttaatgaggt aaagagaaaa tgagcaaaag cacaaacacg ctaagtgccg 1860 gccgtccgag cgcacgcagc agcaaggctg caacgttggc cagcctggca gacacgccag 1920 ccatgaagcg ggtcaacttt cagttgccgg cggaggatca caccaagctg aagatgtacg 1980 cggtacgcca aggcaagacc attaccgagc tgctatctga atacatcgcg cagctaccag 2040 agtaaatgag caaatgaata aatgagtaga tgaattttag cggctaaagg aggcggcatg 2100 gaaaatcaag aacaaccagg caccgacgcc gtggaatgcc ccatgtgtgg aggaacgggc 2160 ggttggccag gcgtaagcgg ctgggttgtc tgccggccct gcaatggcac tggaaccccc 2220 aagcccgagg aatcggcgtg agcggtcgca aaccatccgg cccggtacaa atcggcgcgg 2280 cgctgggtga tgacctggtg gagaagttga aggccgcgca ggccgcccag cggcaacgca 2340 tcgaggcaga agcacgcccc ggtgaatcgt ggcaagcggc cgctgatcga atccgcaaag 2400 aatcccggca accgccggca gccggtgcgc cgtcgattag gaagccgccc aagggcgacg 2460 agcaaccaga ttttttcgtt ccgatgctct atgacgtggg cacccgcgat agtcgcagca 2520 tcatggacgt ggccgttttc cgtctgtcga agcgtgaccg acgagctggc gaggtgatcc 2580 gctacgagct tccagacggg cacgtagagg tttccgcagg gccggccggc atggccagtg 2640 tgtgggatta cgacctggta ctgatggcgg tttcccatct aaccgaatcc atgaaccgat 2700 accgggaagg gaagggagac aagcccggcc gcgtgttccg tccacacgtt gcggacgtac 2760 tcaagttctg ccggcgagcc gatggcggaa agcagaaaga cgacctggta gaaacctgca 2820 ttcggttaaa caccacgcac gttgccatgc agcgtacgaa gaaggccaag aacggccgcc 2880 tggtgacggt atccgagggt gaagccttga ttagccgcta caagatcgta aagagcgaaa 2940 ccgggcggcc ggagtacatc gagatcgagc tagttgattg gatgtaccgc gagatcacag 3000 aaggcaagaa cccggacgtg ctgacggttc accccgatta ctttttgatc gatcccggca 3060 tcggccgttt tctctaccgc ctggcacgcc gcgccgcagg caaggcagaa gccagatggt 3120 tgttcaagac gatctacgaa cgcagtggca gcgccggaga gttcaagaag ttctgtttca 3180 ccgtgcgcaa gctgatcggg tcaaatgacc tgccggagta cgatttgaag gaggaggcgg 3240 ggcaggctgg cccgatccta gtcatgcgct accgcaacct gatcgagggc gaagcatccg 3300 ccggttccta atgtacggag cagatgctag ggcaaattgc cctagcaggg gaaaaaggtc 3360 gaaaaggtct ctttcctgtg gatagcacgt acattgggaa cccaaagccg tacattggga 3420 accggaaccc gtacattggg aacccaaagc cgtacattgg gaaccggtca cacatgtaag 3480 tgactgatat aaaagagaaa aaaggcgatt tttccgccta aaactcttta aaacttatta 3540 aaactcttaa aacccgcctg gcctgtgcat aactgtctgg ccagcgcaca gccgaagagc 3600 tgcaaaaagc gcctaccctt cggtcgctgc gctccctacg ccccgccgct tcgcgtcggc 3660 ctatcgcggc cgctggccgc tcaaaaatgg ctggcctacg gccaggcaat ctaccagggc 3720 gcggacaagc cgcgccgtcg ccactcgacc gccggcgctg aggtctgcct cgtgaagaag 3780 gtgttgctga ctcataccag ggcaatggca acaacgttgc gcaaactatt aactggcgaa 3840 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 3900 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 3960 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 4020 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 4080 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 4140 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 4200 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 4260 cccttaataa gatgatcttc ttgagatcgt tttggtctgc gcgtaatctc ttgctctgaa 4320 aacgaaaaaa ccgccttgca gggcggtttt tcgaaggttc tctgagctac caactctttg 4380 aaccgaggta actggcttgg aggagcgcag tcaccaaaac ttgtcctttc agtttagcct 4440 taaccggcgc atgacttcaa gactaactcc tctaaatcaa ttaccagtgg ctgctgccag 4500 tggtgctttt gcatgtcttt ccgggttgga ctcaagacga tagttaccgg ataaggcgca 4560 gcggtcggac tgaacggggg gttcgtgcat acagtccagc ttggagcgaa ctgcctaccc 4620 ggaactgagt gtcaggcgtg gaatgagaca aacgcggcca taacagcgga atgacaccgg 4680 taaaccgaaa ggcaggaaca ggagagcgca cgagggagcc gccaggggga aacgcctggt 4740 atctttatag tcctgtcggg tttcgccacc actgatttga gcgtcagatt tcgtgatgct 4800 tgtcaggggg gcggagccta tggaaaaacg gctttgccgc ggccctctca cttccctgtt 4860 aagtatcttc ctggcatctt ccaggaaatc tccgccccgt tcgtaagcca tttccgctcg 4920 ccgcagtcga acgaccgagc gtagcgagtc agtgagcgag gaagcggaat atatcctgta 4980 tcacatattc tgctgacgca ccggtgcagc cttttttctc ctgccacatg aagcacttca 5040 ctgacaccct catcagtgcc aacatagtaa gccagtatac actccgctag cgctgaggtc 5100 tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca tcatccagcc 5160 agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag ttggtgattt 5220 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct 5280 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat 5340 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa 5400 acagtaatac aaggggtgtt atgagccata ttcaacggga aacgtcttgc tcgagatcta 5460 tcgatgcatg ccatggtacc cgggagctcg aattcgaagc ttatgcattt ctttccagac 5520 ttgttcaaca ggccagccat tacgctcgtc atcaaaatca ctcgcatcaa ccaaaccgtt 5580 attcattcgt gattgcgcct gagcgagacg aaatacgcga tcgctgttaa aaggacaatt 5640 acaaacagga atcgaatgca accggcgcag gaacactgcc agcgcatcaa caatattttc 5700 acctgaatca ggatattctt ctaatacctg gaatgctgtt ttcccgggga tcgcagtggt 5760 gagtaaccat gcatcatcag gagtacggat aaaatgcttg atggtcggaa gaggcataaa 5820 ttccgtcagc cagtttagtc tgaccatctc atctgtaaca tcattggcaa cgctaccttt 5880 gccatgtttc agaaacaact ctggcgcatc gggcttccca tacaatcgat agattgtcgc 5940 acctgattgc ccgacattat cgcgagccca tttataccca tataaatcag catccatgtt 6000 ggaatttaat cgcggcctcg agcaagacgt ttcccgttga atatggctca taacacccct 6060 tgtattactg tttatgtaag cagacagttt tattgttcat gatgatatat ttttatcttg 6120 tgcaatgtaa catcagagat tttgagacac aacgtggctt tgttgaataa atcgaacttt 6180 tgctgagttg aaggatcaga tcacgcatct tcccgacaac gcagaccgtt ccgtggcaaa 6240 gcaaaagttc aaaatcacca actggtccac ctacaacaaa gctctcatca accgtggctc 6300 cctcactttc tggctggatg atggggcgat tcaggcctgg tatgagtcag caacaccttc 6360 ttcacgaggc agacctcagc gcccgcgagc gagccgccga gctggccggc cagctcaccg 6420 cacaccaggg acaggcggcc gcagttgctg gcacgcctgg caccggccga aaccaaaaca 6480 cccagggcga agaagtaggg cggtggattg gtctggcggt agtggggtct gacgctcagt 6540 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 6600 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactg 6660 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg 6720 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc 6780 atctggcccc agtgctgcaa tgataccgcg agatccacgc tcaccggctc cagatttatc 6840 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc 6900 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag 6960 tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat 7020 ggcttcattc agctccggtt cccaacgatc aaggcgggtt acatgatccc ccatgttgtg 7080 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt 7140 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag 7200 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg 7260 accgagttgc tcttgcccgg cgtcaacacg ggataatacc gcaccacata gcagaacttt 7320 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct 7380 gttgagatcc agctcgatgt aacccactcg tgcacccaac ttatcttcag catcttttac 7440 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat 7500 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat 7560 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca 7620 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat 7680 tatcatgaca ttaacctata aaaataagcg tatcacgagg ccctttcgtc ttcaagaatt 7740 ttataaaccg tggagcgggc aatactgagc tgatgagcaa tttccgttgc accagtgccc 7800 ttctgatgaa gcgtcagcac gacgttcctg tccacggtac gcctgcggcc aaatttgatt 7860 cctttcagct ttgcttcctg tcggccctca ttcgtgcgct ctaggatctc gcctgtcccc 7920 tcagttcagt aatttcctgc atttgcctgt ttccagtcgg tagatattcc acaaaacagc 7980 agggaagcag cgcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc 8040 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt 8100 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag 8160 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc 8220 tctgcgaggc tggccggcta ccgccggcgt aac 8253 // ID AB046924; SV 1; linear; other DNA; STD; SYN; 2223 BP. XX AC AB046924; XX DT 04-JUL-2001 (Rel. 68, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 3) XX DE Synthetic construct gene for Rai-chu 101, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Homo sapiens (human) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. XX RN [1] RP 1-2223 RA Matsuda M., Mochizuki N.; RT ; RL Submitted (04-AUG-2000) to the INSDC. RL Michiyuki Matsuda, International Medical Center of Japan, Department of RL Pathology, Research Institute; Toyama 1-21-1, Shinjuku-ku, Tokyo 162-8655, RL Japan (E-mail:mmatsuda@ri.imcj.go.jp, URL:www.imcj.go.jp, RL Tel:81-3-3202-7181(ex.2833), Fax:81-3-3205-1236) XX RN [2] RX DOI; 10.1038/35082594. RX PUBMED; 11429608. RA Mochizuki N., Yamashita S., Kurokawa K., Ohba Y., Nagai T., Miyawaki A., RA Matsuda M.; RT "Spatio-temporal images of growth-factor-induced activation of Ras and RT Rap1"; RL Nature 411(6841):1065-1068(2001). XX DR MD5; aee27563ef04a13f3c425bc30b78feae. XX FH Key Location/Qualifiers FH FT source 1..2223 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT source 724..1239 FT /organism="Homo sapiens" FT /mol_type="other DNA" FT /note="derived from human H-Ras cDNA" FT /db_xref="taxon:9606" FT source 1258..1500 FT /organism="Homo sapiens" FT /mol_type="other DNA" FT /note="derived from human Raf cDNA" FT /db_xref="taxon:9606" FT CDS 1..2223 FT /codon_start=1 FT /transl_table=11 FT /gene="Rai-chu 101" FT /product="Rai-chu 101" FT /note="fusion protein;enhanced yellow fluorescent protein, FT human H-Ras, human Raf, and enhanced cyan fluorescent FT protein" FT /protein_id="BAB61869.1" FT /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL FT KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD FT GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK FT VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL FT EFVTAAGITLGMDELYKLEMTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSY FT RKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQ FT IKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYT FT LVREIRQHKLRKLNGSGGGTPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVR FT GLQPESCAVFRLLHEHKGKKARLDWNTEAASLIGEELHVDFLGGRMVSKGEELFTGVVP FT ILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFS FT RYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFK FT EDGNILGHKLEYNYISHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGD FT GPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDEL" XX SQ Sequence 2223 BP; 535 A; 677 C; 650 G; 361 T; 0 other; atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180 ctcgtgacca ccttcggcta cggcctgaag tgcttcgccc gctaccccga ccacatgaag 240 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagctc 720 gagatgacgg aatataagct ggtggtggtg ggcgccggcg gtgtgggcaa gagtgcgctg 780 accatccagc tgatccagaa ccattttgtg gacgaatacg accccactat agaggattcc 840 taccggaagc aggtggtcat tgatggggag acgtgcctgt tggacatcct ggataccgcc 900 ggccaggagg agtacagcgc catgcgggac cagtacatgc gcaccgggga gggcttcctg 960 tgtgtgtttg ccatcaacaa caccaagtct tttgaggaca tccaccagta cagggagcag 1020 atcaaacggg tgaaggactc ggatgacgtg cccatggtgc tggtggggaa caagtgtgac 1080 ctggctgcac gcactgtgga atctcggcag gctcaggacc tcgcccgaag ctacggcatc 1140 ccctacatcg agacctcggc caagacccgg cagggagtgg aggatgcctt ctacacgttg 1200 gtgcgtgaga tccggcagca caagctgcgg aagctgaacg gctccggagg tggtacccct 1260 tctaagacaa gcaacactat ccgtgttttc ttgccgaaca agcaaagaac agtggtcaat 1320 gtgcgaaatg gaatgagctt gcatgactgc cttatgaaag cactcaaggt gaggggcctg 1380 caaccagaga gctgtgcagt gttcagactt ctccacgaac acaaaggtaa aaaagcacgc 1440 ttagattgga atactgaagc tgcgtctttg attggagaag aacttcacgt agatttcctg 1500 ggcggccgca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 1560 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 1620 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 1680 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac 1740 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 1800 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 1860 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 1920 ctggggcaca agctggagta caactacatc agccacaacg tctatatcac cgccgacaag 1980 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg 2040 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 2100 gacaaccact acttgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 2160 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 2220 tag 2223 // ID AB046925; SV 1; linear; other DNA; STD; SYN; 2295 BP. XX AC AB046925; XX DT 04-JUL-2001 (Rel. 68, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 3) XX DE Synthetic construct gene for Rai-chu 101X, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Homo sapiens (human) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. XX RN [1] RP 1-2295 RA Matsuda M., Mochizuki N.; RT ; RL Submitted (04-AUG-2000) to the INSDC. RL Michiyuki Matsuda, International Medical Center of Japan, Department of RL Pathology, Research Institute; Toyama 1-21-1, Shinjuku-ku, Tokyo 162-8655, RL Japan (E-mail:mmatsuda@ri.imcj.go.jp, URL:www.imcj.go.jp, RL Tel:81-3-3202-7181(ex.2833), Fax:81-3-3205-1236) XX RN [2] RX DOI; 10.1038/35082594. RX PUBMED; 11429608. RA Mochizuki N., Yamashita S., Kurokawa K., Ohba Y., Nagai T., Miyawaki A., RA Matsuda M.; RT "Spatio-temporal images of growth-factor-induced activation of Ras and RT Rap1"; RL Nature 411(6841):1065-1068(2001). XX DR MD5; 28dc635d98dced914286096c1021ef17. XX FH Key Location/Qualifiers FH FT source 1..2295 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT source 724..1239 FT /organism="Homo sapiens" FT /mol_type="other DNA" FT /note="derived from human H-Ras cDNA" FT /db_xref="taxon:9606" FT source 1258..1500 FT /organism="Homo sapiens" FT /mol_type="other DNA" FT /note="derived from human Raf cDNA" FT /db_xref="taxon:9606" FT source 2233..2292 FT /organism="Homo sapiens" FT /mol_type="other DNA" FT /note="derived from human K-Ras cDNA" FT /db_xref="taxon:9606" FT CDS 1..2295 FT /codon_start=1 FT /transl_table=11 FT /gene="Rai-chu 101X" FT /product="Rai-chu 101X" FT /note="fusion protein;enhanced yellow fluorescent protein, FT human H-Ras, human Raf, enhanced cyan fluorescent protein, FT and human K-Ras" FT /protein_id="BAB61870.1" FT /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL FT KFICTTGKLPVPWPTLVTTFGYGLKCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD FT GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK FT VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL FT EFVTAAGITLGMDELYKLEMTEYKLVVVGAGGVGKSALTIQLIQNHFVDEYDPTIEDSY FT RKQVVIDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQ FT IKRVKDSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYT FT LVREIRQHKLRKLNGSGGGTPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVR FT GLQPESCAVFRLLHEHKGKKARLDWNTEAASLIGEELHVDFLGGRMVSKGEELFTGVVP FT ILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFS FT RYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFK FT EDGNILGHKLEYNYISHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGD FT GPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELGRSRKMSKDGKKK FT KKKSKTKCVIM" XX SQ Sequence 2295 BP; 570 A; 684 C; 668 G; 373 T; 0 other; atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180 ctcgtgacca ccttcggcta cggcctgaag tgcttcgccc gctaccccga ccacatgaag 240 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagctc 720 gagatgacgg aatataagct ggtggtggtg ggcgccggcg gtgtgggcaa gagtgcgctg 780 accatccagc tgatccagaa ccattttgtg gacgaatacg accccactat agaggattcc 840 taccggaagc aggtggtcat tgatggggag acgtgcctgt tggacatcct ggataccgcc 900 ggccaggagg agtacagcgc catgcgggac cagtacatgc gcaccgggga gggcttcctg 960 tgtgtgtttg ccatcaacaa caccaagtct tttgaggaca tccaccagta cagggagcag 1020 atcaaacggg tgaaggactc ggatgacgtg cccatggtgc tggtggggaa caagtgtgac 1080 ctggctgcac gcactgtgga atctcggcag gctcaggacc tcgcccgaag ctacggcatc 1140 ccctacatcg agacctcggc caagacccgg cagggagtgg aggatgcctt ctacacgttg 1200 gtgcgtgaga tccggcagca caagctgcgg aagctgaacg gctccggagg tggtacccct 1260 tctaagacaa gcaacactat ccgtgttttc ttgccgaaca agcaaagaac agtggtcaat 1320 gtgcgaaatg gaatgagctt gcatgactgc cttatgaaag cactcaaggt gaggggcctg 1380 caaccagaga gctgtgcagt gttcagactt ctccacgaac acaaaggtaa aaaagcacgc 1440 ttagattgga atactgaagc tgcgtctttg attggagaag aacttcacgt agatttcctg 1500 ggcggccgca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc 1560 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat 1620 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc 1680 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcagccg ctaccccgac 1740 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc 1800 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc 1860 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc 1920 ctggggcaca agctggagta caactacatc agccacaacg tctatatcac cgccgacaag 1980 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg 2040 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc 2100 gacaaccact acttgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat 2160 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg 2220 ggccgctcta gaaagatgag caaagatggt aaaaagaaga aaaagaagtc aaagacaaag 2280 tgtgtaatta tgtaa 2295 // ID AB049968; SV 1; circular; other DNA; STD; SYN; 2657 BP. XX AC AB049968; XX DT 16-APR-2001 (Rel. 67, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 3) XX DE Expression vector pLEAD4 DNA, complete sequence. XX KW . XX OS Expression vector pLEAD4 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-2657 RA Ishida M., Oshima T., Watahiki M.; RT ; RL Submitted (13-OCT-2000) to the INSDC. RL Masami Ishida, Tokyo University of Fisheries, Laboratory of Marine RL Biochemistry; Konan 4, Minato-ku, Tokyo 108-8477, Japan RL (E-mail:ishida@tokyo-u-fish.ac.jp, Tel:81-3-5463-0586, Fax:81-3-5463-0589) XX RN [2] RX PUBMED; 12097161. RA Ishida M., Oshima T.; RT "Effective structure of a leader open reading frame for enhancing the RT expression of GC-rich genes"; RL J. Biochem. 132(1):63-70(2002). XX DR MD5; 7d02633e4be181a4d33b44b1ecc4f1d4. XX FH Key Location/Qualifiers FH FT source 1..2657 FT /organism="Expression vector pLEAD4" FT /lab_host="Escherichia coli" FT /mol_type="other DNA" FT /db_xref="taxon:139774" FT regulatory 4..9 FT /regulatory_class="ribosome_binding_site" FT CDS 18..71 FT /codon_start=1 FT /transl_table=11 FT /note="The short ORF, termed 'leader ORF (precursor)', FT enhances the translation initiation of a downstream gene FT through a 4 bp-overlapping by the ATGA motif." FT /protein_id="BAB41065.1" FT /translation="MTMIDIRRIYVCIELGT" FT regulatory 36..41 FT /regulatory_class="ribosome_binding_site" FT misc_feature 45..87 FT /note="multiple cloning sites: Eco105I, EcoT22I, SacI, FT KpnI, StuI, BamHI, and HindIII" FT CDS 606..1466 FT /codon_start=1 FT /transl_table=11 FT /gene="bla" FT /product="beta-lactamhydrolase" FT /EC_number="3.5.2.6" FT /protein_id="BAB41066.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT regulatory 2586..2613 FT /note="tac promoter" FT /regulatory_class="promoter" XX SQ Sequence 2657 BP; 676 A; 625 C; 690 G; 666 T; 0 other; ttcaggagga ttatcatatg accatgattg atatcaggag gatttacgta tgcattgagc 60 tcggtaccta ggcctggatc caagcttggc tgttttggcg gatgagagaa gattttcagc 120 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc 180 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc 240 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg 300 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct 360 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg 420 gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga aggccatcct 480 gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa atacattcaa 540 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 600 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 660 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 720 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 780 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 840 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 900 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 960 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 1020 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 1080 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 1140 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 1200 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 1260 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 1320 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 1380 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 1440 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 1500 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 1560 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 1620 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 1680 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 1740 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 1800 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 1860 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 1920 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 1980 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 2040 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 2100 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 2160 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 2220 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 2280 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 2340 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 2400 cggggcttat cgactgcacg gtgcaccaat gcttctggcg tcaggcagcc atcggaagct 2460 gtggtatggc tgtgcaggtc gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc 2520 cgttctggat aatgtttttt gcgccgacat cataacggtt ctggcaaata ttctgaaatg 2580 agctgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg gataacaatt 2640 tcacacagga aacagaa 2657 // ID AB049969; SV 1; circular; other DNA; STD; SYN; 2659 BP. XX AC AB049969; XX DT 16-APR-2001 (Rel. 67, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 3) XX DE Expression vector pLEAD5 DNA, complete sequence. XX KW . XX OS Expression vector pLEAD5 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-2659 RA Ishida M., Oshima T., Watahiki M.; RT ; RL Submitted (13-OCT-2000) to the INSDC. RL Masami Ishida, Tokyo University of Fisheries, Laboratory of Marine RL Biochemistry; Konan 4, Minato-ku, Tokyo 108-8477, Japan RL (E-mail:ishida@tokyo-u-fish.ac.jp, Tel:81-3-5463-0586, Fax:81-3-5463-0589) XX RN [2] RX PUBMED; 12097161. RA Ishida M., Oshima T.; RT "Effective structure of a leader open reading frame for enhancing the RT expression of GC-rich genes"; RL J. Biochem. 132(1):63-70(2002). XX DR MD5; 096aded5f9062b54bd2c3a25f338683d. XX FH Key Location/Qualifiers FH FT source 1..2659 FT /organism="Expression vector pLEAD5" FT /lab_host="Escherichia coli" FT /mol_type="other DNA" FT /db_xref="taxon:139775" FT regulatory 4..9 FT /regulatory_class="ribosome_binding_site" FT CDS 18..53 FT /codon_start=1 FT /transl_table=11 FT /note="The short ORF, termed 'leader ORF (precursor)', FT enhances the translation initiation of a downstream gene FT through a 1 bp-overlapping in the TGATG motif." FT /protein_id="BAB41067.1" FT /translation="MTMITDIRRIT" FT regulatory 39..44 FT /regulatory_class="ribosome_binding_site" FT misc_feature 47..89 FT /note="multiple cloning sites: BbrPI, EcoT22I, SacI, KpnI, FT StuI, BamHI, and HindIII" FT CDS 608..1468 FT /codon_start=1 FT /transl_table=11 FT /gene="bla" FT /product="beta-lactamhydrolase" FT /EC_number="3.5.2.6" FT /protein_id="BAB41068.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT regulatory 2588..2615 FT /note="tac promoter" FT /regulatory_class="promoter" XX SQ Sequence 2659 BP; 677 A; 627 C; 691 G; 664 T; 0 other; ttcaggagga ttatcatatg accatgatta ctgatatcag gaggatcacg tgatgcatga 60 gctcggtacc taggcctgga tccaagcttg gctgttttgg cggatgagag aagattttca 120 gcctgataca gattaaatca gaacgcagaa gcggtctgat aaaacagaat ttgcctggcg 180 gcagtagcgc ggtggtccca cctgacccca tgccgaactc agaagtgaaa cgccgtagcg 240 ccgatggtag tgtggggtct ccccatgcga gagtagggaa ctgccaggca tcaaataaaa 300 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 360 ctcctgagta ggacaaatcc gccgggagcg gatttgaacg ttgcgaagca acggcccgga 420 gggtggcggg caggacgccc gccataaact gccaggcatc aaattaagca gaaggccatc 480 ctgacggatg gcctttttgc gtttctacaa actcttttgt ttatttttct aaatacattc 540 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 600 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 660 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 720 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 780 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 840 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 900 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 960 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 1020 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 1080 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 1140 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 1200 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 1260 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 1320 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 1380 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 1440 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 1500 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 1560 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 1620 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 1680 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 1740 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 1800 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 1860 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 1920 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 1980 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 2040 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 2100 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 2160 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 2220 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 2280 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 2340 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 2400 agcggggctt atcgactgca cggtgcacca atgcttctgg cgtcaggcag ccatcggaag 2460 ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc aaggcgcact 2520 cccgttctgg ataatgtttt ttgcgccgac atcataacgg ttctggcaaa tattctgaaa 2580 tgagctgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag cggataacaa 2640 tttcacacag gaaacagaa 2659 // ID AB052891; SV 2; circular; genomic DNA; STD; SYN; 5240 BP. XX AC AB052891; XX DT 11-JAN-2001 (Rel. 66, Created) DT 11-MAY-2005 (Rel. 83, Last updated, Version 2) XX DE Cloning vector pCA24N DNA, complete genome. XX KW . XX OS Cloning vector pCA24N OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-5240 RA Kitagawa M., Hirai A., Mori H.; RT ; RL Submitted (21-DEC-2000) to the INSDC. RL Hirotada Mori, Nara Institute of Science & Technology, Research & Education RL Center of Genetic Information; Takayama 8916-5, Ikoma, Nara, 630-0101, RL Japan (E-mail:hmori@gtc.naist.jp, Tel:81-743-72-5662, Fax:81-743-72-5669) XX RN [2] RA Kitagawa M.; RT "Archive Vector"; RL Unpublished. XX DR MD5; 6faa8df39bdedf06e47f9276c1d558c3. DR EuropePMC; PMC2713508; 19372223. XX FH Key Location/Qualifiers FH FT source 1..5240 FT /organism="Cloning vector pCA24N" FT /mol_type="genomic DNA" FT /db_xref="taxon:146876" FT regulatory 71..163 FT /standard_name="p-T5-lac" FT /regulatory_class="promoter" FT CDS 185..250 FT /codon_start=1 FT /transl_table=11 FT /gene="his-tag" FT /product="6xHIS" FT /protein_id="BAB20664.1" FT /translation="MRGSHHHHHHTDPALRPMRPQ" FT misc_feature <234..959 FT /gene="gcn4" FT /product="GFPuv4" FT regulatory 1139..1320 FT /gene="rrnB-T1" FT /regulatory_class="terminator" FT CDS 1761..2420 FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="CAT" FT /protein_id="BAB20666.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT rep_origin 3556 FT CDS 3879..4961 FT /codon_start=1 FT /transl_table=11 FT /gene="lacI^q" FT /product="LacI" FT /protein_id="BAB20667.1" FT /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL FT NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV FT EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH FT EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA FT MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC FT YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR FT ALADSLMQLARQVSRLESGQ" XX SQ Sequence 5240 BP; 1362 A; 1262 C; 1306 G; 1310 T; 0 other; ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 60 tcgtcttcac ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat 120 aatagattca attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt 180 aactatgaga ggatctcacc atcaccatca ccatacggat ccggccctga ggcctatgcg 240 gccgcagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg 300 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg 360 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac 420 ttgtcactac tctgacgtat ggtgttcaat gcttttcccg ttatccggat catatgaaac 480 ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaacgc actatatctt 540 tcaaagatga cgggaactac aagacgcgtg ctgaagtcaa gtttgaaggt gatacccttg 600 ttaatcgtat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt ctcggacaca 660 aactcgagta caactataac tcacacaatg tatacatcac ggcagacaaa caaaagaatg 720 gaatcaaagc taacttcaaa attcgccaca acattgaaga tggatccgtt caactagcag 780 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt 840 acctgtcgac acaatctgcc cttttgaaag atcccaacga aaagcgtgac cacatggtcc 900 ttcttgagtt tgtaactgct gctgggatta cacatggcat ggatgaacta tacaaataag 960 cggccgctaa gggtcgacct gcagccaagc ttaattagct gagcttggac tcctgttgat 1020 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg 1080 ggcgtttttt attggtgaga atccaagcta ggggaattca tcgtgactga ctgacgatct 1140 gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg 1200 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg 1260 gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata 1320 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 1380 taatggtttc ttaggatcct gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc 1440 agtagctgaa caggagggac agctgataga aacagaagcc actggagcac ctcaaaaaca 1500 ccatcataca ctaaatcagt aagttggcag catcacccga cgcactttgc gccgaataaa 1560 tacctgtgac ggaagatcac ttcgcagaat aaataaatcc tggtgtccct gttgataccg 1620 ggaagccctg ggccaacttt tggcgaaaat gagacgttga tcggcacgta agaggttcca 1680 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt 1740 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat 1800 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata 1860 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 1920 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc 1980 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg 2040 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc 2100 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt 2160 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca 2220 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg 2280 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg 2340 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg 2400 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtg 2460 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgaaag caaattcgac 2520 ccggtcgtcg gttcagggca gggtcgttaa atagccgctt atgtctattg ctggtttacc 2580 ggtttattga ctaccggaag cagtgtgacc gtgtgcttct caaatgcctg aggccagttt 2640 gctcaggctc tccccgtgga ggtaataatt gacgatatga tcatttattc tgcctcccag 2700 agcctgataa aaacggttag gatcggagtc aggcaactat ggatgaacga aatagacaga 2760 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 2820 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 2880 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 2940 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 3000 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 3060 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 3120 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 3180 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 3240 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 3300 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 3360 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 3420 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 3480 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 3540 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 3600 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 3660 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 3720 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 3780 tttcacaccg cataaattcc gacaccatcg aatggtgcaa aacctttcgc ggtatggcat 3840 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg 3900 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca 3960 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca 4020 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca 4080 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg 4140 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta 4200 aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc 4260 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc 4320 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc 4380 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc 4440 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca 4500 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac 4560 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc 4620 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata 4680 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca 4740 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct 4800 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa 4860 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 4920 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 4980 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 5040 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgga 5100 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa 5160 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga 5220 tcgcccttcc caacagttgc 5240 // ID AB053999; SV 1; linear; other DNA; STD; SYN; 991 BP. XX AC AB053999; XX DT 13-JUL-2001 (Rel. 68, Created) DT 01-JUN-2003 (Rel. 76, Last updated, Version 3) XX DE Synthetic construct f beta/scuPA-32k gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-991 RA Jiao J.W.; RT ; RL Submitted (12-JAN-2001) to the INSDC. RL Jian Wei Jiao, Peking Univeristy, Colledge of life sciences; Peking RL University, Building48 Room2072, Beijing 100871, P. R. China RL (E-mail:jjw@pubms.pku.edu.cn, Tel:8610-62761784) XX RN [2] RA Jiao J.W., Ru B.G.; RT "Construction and characterization of a recombinant chimeric plasminogen RT activator consisting of a fibrin peptide and a low molecular weight RT single-chain urokinase"; RL Unpublished. XX DR MD5; d6ccd9fd1e34ba6ac782637614366fef. XX FH Key Location/Qualifiers FH FT source 1..991 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..909 FT /codon_start=1 FT /transl_table=11 FT /gene="f beta/scuPA-32k" FT /note="fusion gene, a recombinant chimeric plasminogen FT activator consisting of a fibrin peptide and a low FT molecular weight single-chain urokinase" FT /protein_id="BAB62043.1" FT /translation="MGHRPLDKKREEAPSLRPAPPPISGGGYRGGGIDLKFQCGQKTLR FT PRFKIIGGEFTTIENQPWFAAIYRRHRGGSVTYVCGGSLMSPCWVISATHCFIDYPKKE FT DYIVYLGRSRLNSNTQGEMKFEVENLILHKDYSADTLAHHNDIALLKIRSKEGRCAQPS FT RTIQTICLPSMYNDPQFGTSCEITGFGKENSSDYLYPEQLKMTVVKLISHRECQQPHYY FT GSEVTTKMLCAADPQWKTDSCQGDSGGPLVCSLQGRMTLTGIVSWGRGCALKDKPGVYT FT RVSHFLPWIRSHTKEENGLAL" XX SQ Sequence 991 BP; 241 A; 279 C; 264 G; 207 T; 0 other; atgggtcatc gaccccttga caagaaaaga gaagaggctc ccagcctgcg acctgcccca 60 ccgcccatca gtggaggtgg ctatcgggga ggcggaatcg atttaaaatt tcagtgtggc 120 caaaagactc tgaggccccg ctttaagatt attgggggag aattcaccac catcgagaac 180 cagccctggt ttgcggccat ctacaggagg caccgggggg gctctgtcac ctacgtgtgt 240 ggaggcagcc tcatgagccc ttgctgggtg atcagcgcca cacactgctt cattgattac 300 ccaaagaagg aggactacat cgtctacctg ggtcgctcaa ggcttaactc caacacgcaa 360 ggggagatga agtttgaggt ggaaaacctc atcctacaca aggactacag cgctgacacg 420 cttgctcacc acaacgacat tgccttgctg aagatccgtt ccaaggaggg caggtgtgcg 480 cagccatccc ggactataca gaccatctgc ctgccctcga tgtataacga tccccagttt 540 ggcacaagct gtgagatcac tggctttgga aaagagaatt catccgacta tctctatccg 600 gagcagctga aaatgactgt tgtgaagctg atttcccacc gggagtgtca gcagccccac 660 tactacggct ctgaagtcac caccaaaatg ctatgtgctg ctgaccccca atggaaaaca 720 gattcctgcc agggagactc agggggaccc ctcgtctgtt ccctccaagg ccgcatgact 780 ttgactggaa ttgtgagctg gggccgtgga tgtgccctga aggacaagcc aggcgtctac 840 acgagagtct cacacttctt accctggatc cgcagtcaca ccaaggaaga gaatggcctg 900 gccctctgag ggtccccagg gaggaaacgg gcaccacccg ctttcttgct ggttgtcatt 960 tttgcagtag agtcatctcc atcagaagct t 991 // ID AB055064; SV 1; linear; other DNA; STD; SYN; 7635 BP. XX AC AB055064; XX DT 06-MAR-2001 (Rel. 67, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic transposable element dAc-I-RS DNA, complete sequence. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-7635 RA Machida C., Machida Y.; RT ; RL Submitted (25-JAN-2001) to the INSDC. RL Yasunori Machida, Nagoya University, Division of Biological Science, RL Graduate School of Science; Chikusa-ku, Furo-cho, Nagoya, Aichi 464-8602, RL Japan (E-mail:yas@bio.nagoya-u.ac.jp, Tel:81-52-789-5040, RL Fax:81-52-789-2966) XX RN [2] RA Semiarti E., Onouchi H., Torikai S., Ishikawa T., Machida Y., Machida C.; RT "The transposition pattern of the Ac element in tobacco cultured cells"; RL Unpublished. XX RN [3] RX DOI; 10.1073/pnas.94.16.8675. RX PUBMED; 11038561. RA Machida C., Onouchi H., Koizumi J., Hamada S., Semiarti E., Torikai S., RA Machida Y.; RT "Characterization of the transposition pattern of the Ac element in RT Arabidopsis thaliana using endonuclease I-SceI"; RL Proc. Natl. Acad. Sci. U.S.A. 94(16):8675-8680(1997). XX DR MD5; 2a04d78e1294ca4c3e7bf93467b99169. XX FH Key Location/Qualifiers FH FT source 1..7635 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="non-autonomous derivative of Ac" FT /note="transposable element dAc-I-RS" FT /db_xref="taxon:32630" FT misc_feature 1..1789 FT /note="sequence of the Ac element in maize, containing the FT terminal inverted repeat" FT misc_feature 1807..1864 FT /note="RS recombination site recognized by R protein from FT Zygosaccharomyces rouxii" FT regulatory 2016..2380 FT /note="the 35S promoter from cauliflower mosaic virus" FT /regulatory_class="promoter" FT CDS 2390..3415 FT /codon_start=1 FT /transl_table=11 FT /gene="HPT" FT /product="hygromycin phosphotransferase" FT /protein_id="BAB33367.1" FT /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG FT YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP FT ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ FT TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF FT GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG FT NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK FT E" FT regulatory 3436..4560 FT /note="the terminator of the gene for nopaline synthase" FT /regulatory_class="terminator" FT misc_feature 4575..4632 FT /note="RS recombination site recognized by R protein from FT Zygosaccharomyces rouxii" FT misc_feature 4683..4700 FT /note="cleavage site for endonuclease I-SceI" FT misc_feature 5149..5172 FT /note="cos sequence" FT misc_feature 6496..7635 FT /note="sequence of the Ac element in maize, containing the FT terminal inverted repeat" XX SQ Sequence 7635 BP; 1960 A; 1915 C; 1783 G; 1977 T; 0 other; cagggatgaa agtaggatgg gaaaatcccg taccgaccgt tatcgtataa ccgattttgt 60 tagttttatc ccgatcgatt tcgaacccga ggtaaaaaac gaaaacggaa cggaaacggg 120 atatacaaaa cggtaaacgg aaacggaaac ggtagagcta gtttcccgac cgtttcaccg 180 ggatcccgtt tttaatcggg atgatcccgt ttcgttaccg tattttctaa ttcgggatga 240 ctgcaatatg gccagctcca actcccatcc ataaccactg aggcccagcc catgtaagaa 300 atacctagcg aacgctgctc tgcctctctc ccaggcggcc aggcaccaca cgagtaacag 360 catcacacat tcacacgccg ccacgcgccc acgccggagt ccggacgccg ccagccgcac 420 gccgacgccg gcgacgcgtc tcgctctcgc ctgctctctc cgactctccc tgtctcccag 480 ccggccggcc gctgggctgc accaggcacc acacgcggtg acggccgtga cgcggcacgc 540 cggacgcaga cgccgccatc cacggtccgc cctccactcc actgctcgcg actcgcccat 600 ccgcgccgcg gtccgcccat ccgcccagac ctccactcca ctgctcgccc atccgcggtc 660 cgcccatccg ccatccgcca tctgcggtca gcggtcctcc agacctccac tgctcggcgc 720 tcgcccatcc ggccatccgc ggtctccctg tctccacggc tgctcacagg ctcacagcac 780 ttagcagtac agcacgtcag caccattgca ccaagctgtt gtgtcatttg tgtgctgtcc 840 aggggctctg caacacctgc tgattgctgt ccagccgtcc aggtgctcac aagtcacagc 900 agtacagcac caagctgatt gctgaacacc tgctgtccag ggctctgctc tccacttcgg 960 ctagccggct acgactccat tcctcagatg acgcctccgg ttggaaataa tcctccctca 1020 ggctcagcca taagattggc caagttgatg tctaccacaa gagcgccttc tactcgcaaa 1080 acaaattccg tattctctgc atatgctcaa ggtatatatt agaaaaacag tagcaatagc 1140 attagcatta ctaattggtt gtagattggg aagcatcata ttgactgtag aataatacga 1200 aaaatctgtt tataacaggg ttgaaaagaa aagctgaagc ctcttctagt cggattcaga 1260 atgtacgtgc acgtgcgcgt gggcatggat gtggccgcac atcaccatca tcatcaacag 1320 ctgaggccga gaggcatttt attcagagtg taagcagtag taatgcaaat ggtacagcta 1380 cagatccgag tcaagatgat atggctattg ttcatgaacc acaaccacaa ccacaaccac 1440 aaccagaacc acaaccacag ccacaacctg aacccgaaga agaagcacca cagaagaggg 1500 caaagaagtg cacatcggat gtatggcagc atttcaccaa gaaggaaatt gaagtggagg 1560 tcgatggaaa gaaatacgtt caggtatggg gacattgcaa ctttcctaat tgcaaggcta 1620 agtatagggc tgagggtcat catggaacaa gcggatttcg aaatcacttg agaacatcac 1680 atagtttagt taaaggtcag ttgtgtctaa aaagtgaaaa ggatcatggc aaagacataa 1740 atctcattga gccttataag tacgatgaag tggttagcct aaagaagctc taggataccg 1800 tcgacccgag atcatatcac tgtggacgtt gatgaaagaa tacgttattc tttcatcaaa 1860 tcgtggtcga cctcgaccac cgcggtggcg gccgctctag aactagtgga tcccccgggc 1920 tgcaggaatt cgatatcaag ctcgcgtcct agagatccgt caacatggtg gagcacgaca 1980 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga 2040 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc 2100 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata 2160 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac 2220 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt 2280 gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct tcgcaagacc 2340 cttcctctat ataaggaagt tcatttcatt tggagaggac gaccccgata tgaaaaagcc 2400 tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga 2460 cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg taggagggcg 2520 tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc gttatgttta 2580 tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg gggaattcag 2640 cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc aagacctgcc 2700 tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc 2760 ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata 2820 cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc actggcaaac 2880 tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg 2940 ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct ccaacaatgt 3000 cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga tgttcgggga 3060 ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt gtatggagca 3120 gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc 3180 gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg gcaatttcga 3240 tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt 3300 cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct gtgtagaagt 3360 actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg aatagagtag 3420 atgccgaccg ggatcgatcc aacacttacg tttgcaacgt ccaagagcaa atagaccacg 3480 aacgccggaa ggttgccgca gcgtgtggat tgcgtctcaa ttctctcttg caggaatgca 3540 atgatgaata tgatactgac tatgaaactt tgagggaata ctgcctagca ccgtcacctc 3600 ataacgtgca tcatgcatgc cctgacaaca tggaacatcg ctatttttct gaagaattat 3660 gctcgttgga ggatgtcgcg gcaattgcag ctattgccaa catcgaacta cccctcacgc 3720 atgcattcat caatattatt catgcgggga aaggcaagat taatccaact ggcaaatcat 3780 ccagcgtgat tggtaacttc agttccagcg acttgattcg ttttggtgct acccacgttt 3840 tcaataagga cgagatggtg gagtaaagaa ggagtgcgtc gaagcagatc gttcaaacat 3900 ttggcaataa agtttcttaa gattgaatcc tgttgccggt cttgcgatga ttatcatata 3960 atttctgttg aattacgtta agcatgtaat aattaacatg taatgcatga cgttatttat 4020 gagatgggtt tttatgatta gagtcccgca attatacatt taatacgcga tagaaaacaa 4080 aatatagcgc gcaaactagg ataaattatc gcgcgcggtg tcatctatgt tactagatcg 4140 atcaaacttc ggtactgtgt aatgacgatg agcaatcgag aggctgacta acaaaaggta 4200 tgcccaaaaa caacctctcc aaactgtttc gaattggaag tttctgctca tgccgacagg 4260 cataacttag atattcgcgg gctattccca ctaattcgtc ctgctggttt gcgccaagat 4320 aaatcagtgc atctccttac aagttcctct gtcttgtgaa atgaactgct gactgccccc 4380 caagaaagcc tcctcatctc ccagttggcg gcggctgata caccatcgaa aacccacgtc 4440 cgaacacttg atacatgtgc ctgagaaata ggcctacgtc caagagcaag tcctttctgt 4500 gctcgtcgga aattcctctc ctgtcagacg gtcgtgcgca tgtcttgcgt tgatgaagct 4560 cgataccgtc gaccacgatt tgatgaaaga ataacgtatt ctttcatcaa cgtccacagt 4620 gatatgatct cgggtcgacc tcgccaccgc ggtggcggcc gctctagaac tagtggatcc 4680 gctagggata acagggtaat atagatcccc gggctgcagg aattcgatat caagctctag 4740 aactagtgga tctgcctcgc tggcctgccg cagttcttca acctcccggc gcagcttttc 4800 gttctcaatt tcagcatccc tttcggcata ccattttatg acggcggcag agtcataaag 4860 cacctcatta cccttgccac cgcctcgcag aacgggcatt ccctgttcct gccagttctg 4920 aatggtacgg atactcgcac cgaaaatgtc agccagctgc tttttgttga cttccattgt 4980 tcattccacg gacaaaaaca gagaaaggaa acgacagagg ccaaaaagct cgctttcagc 5040 acctgtcgtt tcctttcttt tcagagggta ttttaaataa aaacattaag ttatgacgaa 5100 gaagaacgga aacgccttaa accggaaaat tttcataaat agcgaaaacc cgcgaggtcg 5160 ccgccccgta acaaggcgga tcgccggaaa ggacccgcaa atgataataa ttatcaattg 5220 catactatcg acggcactgc tgccagataa caccaccggg gaaacattcc atcatgatgg 5280 ccgtgcggac ataggaagcc agttcatcca tcgctttctt gtctgctgcc atttgctttg 5340 tgacatccag cgccgcacat tcagcagcgt ttttcagcgc gttttcgatc aacgtttcaa 5400 tgttggtatc aacaccaggt ttaactttga acttatcggc actgacggtt accttgttct 5460 gcgctggctc atcacgctgg ataccaaggc tgatgttgta gatattggtc accggctgag 5520 gtgtttcgat tgccgctgcg tggatagcac catttgcgat agcggcgtcc ttgatgaatg 5580 acactccatt gcgaataagt tcgaaggaga cggtgtcacg aatgcgctgg tccagctcgt 5640 cgattgcctt ttgtgcagca gaggtatcaa tctcaacgcc aagcgtcatc gaagcgcaat 5700 attgctgctc accaaaacgc gtattgacca ggtgttcaac ggcaaatttc tgcccttctg 5760 atgtcagaaa ggtaaagtga ttttctttct ggtattcagt tgctgtgtgt ctggtttcag 5820 caaaaccaag ctcgcgcaat tcggctgtgc cagatttaga aggcagatca ccagacagca 5880 acgcgccacg gaaaaacagc gcatacagaa catccgtcgc cgcgccggac aacgtgataa 5940 ttttatgacc catgatttat ttccttttag acgtgagcct gtcgcacagc aaagccgccg 6000 aaagttaacg gtttgcccag gctcacaact gaaagacttt ctacggtgtg cgcgtgcgat 6060 gcgcgtagaa gactgattta tcaacctgtc tttatatcag gattcattac ctgactattt 6120 gtgggtaaag ttcgtagtgc gctgatcgtg caaaatgatt ttagttggga acagttcgca 6180 actctgtccc ataaaaatca gcatattccc atctatccca tatccagcgc attgaccatc 6240 gggatactga agggagattc catcatctct tagaaagatc accatctctt ttgtttcaat 6300 ttgcatatag ctacctggag gatttatgaa tacaaggatt ttcatggact attaccatga 6360 gattgatttt ccatctttat tcgcgagagc agtggaaagc gatgacgatg tgggtactac 6420 attgcgcatt cacctacttt gtgagcgcat ggtcgaagca tggatatgcg catgctgtga 6480 ctgccaagat cccccctaga aaaggtgaat gcatatatgt tataatgaag ttccaattta 6540 tagttattca acaattattt tacttatatt gatgcatatt tgtgtcattc aaggtgctac 6600 atattttcca acaatgattg gtgatctcga ggtgctagac tctgttattg ctgctgcaac 6660 aaatcatgag aatcatatgg atgaggtatt taaagattat tatttacttc gtgcatgggc 6720 tattaatttg ctattattca ctactgtttt gatgcatggg ctgtttgctg tcgccttgtt 6780 ttgatgcatg cgccttgctg cccagccgtg ttatactccc tgcatggctg gcattaacag 6840 atttttgatc tcactgcatg cgccttgtcg ccttgttttg attggctgct agctgctagc 6900 tgttaggctc ccagctgtta ggcgctagct gctagctgcc tagctcccag ccgtgttagt 6960 tcacagattc atgttctcct atatgtattt atttatactc cctgcatatc aattcatgtt 7020 cttctatatg tatttattta tccaaaactg acttattttt gtgtattaac aggatgaaga 7080 cgcaatagaa ttttctaaga ataatgaaga tgtagcaagt ggctcctctc catgagcaat 7140 gtgtcttatg tttgttgaca gatgagcctt ggttgtaata gtttatgcat gctaagtgat 7200 ccagatgtga gcaagtgatt atgaatatgt gttttaaact ttatattgtg tcatgtgtgc 7260 tagtagactt atatggcttc ttatgttagc caagagccca agacttatca cttatgtgct 7320 acattaaact atgtgtgctc cagatttata tggattttat ctatgtttaa ttaagacttg 7380 tgtttacaat tttttatatt tgtttttaag ttttgaatat atgttttcat gtgtgatttt 7440 accgaacaaa aataccggtt cccgtccgat ttcgacttta acccgaccgg atcgtatcgg 7500 ttttcgatta ccgtatttat cccgttcgtt ttcgttaccg gtatatcccg ttttcgtttc 7560 cgtcccgcaa gttaaatatg aaaatgaaaa cggtagaggt attttaccga ccgttaccga 7620 ccgttttcat cccta 7635 // ID AB055650; SV 1; circular; other DNA; STD; SYN; 4506 BP. XX AC AB055650; XX DT 11-SEP-2001 (Rel. 69, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 3) XX DE Thermosensitive suicide vector pSET4s DNA, complete sequence. XX KW . XX OS Thermosensitive suicide vector pSET4s OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-4506 RA Takamatsu D., Osaki M., Sekizaki T.; RT ; RL Submitted (08-FEB-2001) to the INSDC. RL Daisuke Takamatsu, National Institute of Animal Health, Laboratory of RL Molecular Bacteriology; Kannondai 3-1-1, Tsukuba, Ibaraki 305-0856, Japan RL (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, Fax:81-298-38-7743) XX RN [2] RX DOI; 10.1006/plas.2001.1532. RX PUBMED; 11591139. RA Takamatsu D., Osaki M., Sekizaki T.; RT "Thermosensitive suicide vectors for gene replacement in Streptococcus RT suis"; RL Plasmid 46(2):140-148(2001). XX DR MD5; e523a8e89d8041865de0befacada6e4c. XX FH Key Location/Qualifiers FH FT source 1..4506 FT /organism="Thermosensitive suicide vector pSET4s" FT /mol_type="other DNA" FT /db_xref="taxon:152114" FT CDS 528..689 FT /codon_start=1 FT /transl_table=11 FT /gene="orfC" FT /product="ORFC" FT /protein_id="BAB64880.1" FT /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA FT RKESEQKK" FT CDS 756..1454 FT /codon_start=1 FT /transl_table=11 FT /gene="repAts" FT /product="RepAts" FT /protein_id="BAB64881.1" FT /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE FT KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD FT YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV FT DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK FT VLDAETGEIK" FT CDS complement(1803..2126) FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="LacZ'" FT /protein_id="BAB64882.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS FT DAA" FT CDS 3637..4404 FT /codon_start=1 FT /transl_table=11 FT /gene="spc" FT /product="spectinomycin adenyltransferase" FT /protein_id="BAB64883.1" FT /translation="MRRIYLNTYEQINKVKKILRKHLKNNLIGTYMFGSGVESGLKPNS FT DLDFLVVVSEPLTDQSKEILIQKIRPISKKIGDKSNLRYIELTIIIQQEMVPWNHPPKQ FT EFIYGEWLQELYEQGYIPQKELNSDLTIMLYQAKRKNKRIYGNYDLEELLPDIPFSDVR FT RAIMDSSEELIDNYQDDETNSILTLCRMILTMDTGKIIPKDIAGNAVAESSPLEHRERI FT LLAVRSYLGENIEWTNENVNLTINYLNNRLKKL" XX SQ Sequence 4506 BP; 1488 A; 779 C; 991 G; 1248 T; 0 other; actagttatc ggcataatcg ttaaaacagg cgttatcgta gcgtaaaagc ccttgagcgt 60 agcgtggctt tgcagcgaag atgttgtctg ttagattatg aaagccgatg actgaatgaa 120 ataataagcg cagcgccctt ctatttcggt tggaggaggc tcaagggagt atgagggaat 180 gaaattccct catgggtttg attttaaaaa ttgcttgcaa ttttgccgag cggtagcgct 240 ggaaaatttt tgaaaaaaat ttggaatttg gaaaaaaatg gggggaaagg aagcgaattt 300 tgcttccgta ctacgacccc ccattaagtg ccgagtgcca atttttgtgc caaaaacgct 360 ctatcccaac tggctcaagg gtttaagggg tttttcaatc gccaacgaat cgccaacgtt 420 ttcgccaacg ttttttataa atctatattt aagtagcttt attgttgttt ttatgattac 480 aaagtgatac actaacttta taaaattatt tgattggagt tttttaaatg gtgatttcag 540 aatcgaaaaa aagagttatg atttctctga caaaagagca agataaaaaa ttaacagata 600 tggcgaaaca aaaaggtttt tcaaaatctg cggttgcggc gttagctata gaagaatatg 660 caagaaagga atcagaacaa aaaaaataag cgaaagctcg cgtttttaga aggatacgag 720 ttttcgctac ttgtttttga taaggtaatt atatcatggc tattaaaaat actaaagcta 780 gaaattttgg atttttatta tatcctgact caattcctaa tgattggaaa gaaaaattag 840 agagtttggg cgtatctatg gctgtcagtc ctttacacga tatggacgaa aaaaaagata 900 aagatacatg gaataatagt aatattatac aaaatggaaa gcactataaa aaaccacact 960 atcacgttat atatattgca cgaaatcctg taacaataga aagcgttagg aacaagatta 1020 agcgaaaatt ggggaatagt tcagttgctc atgttgagat acttgattat atcaaaggtt 1080 catatgaata tttgactcat gaatcaaagg acgctattgc taagaataaa catatatacg 1140 acaaaaaaga tattttgaac attaatgatt ttgatattga ccgctatata acacttgatg 1200 aaagccaaaa aagagaattg aagaatttac ttttagatat agtggatgac tataatttgg 1260 taaatacaaa agatttaatg gcttttattc gccttagggg agcggagttt ggaattttaa 1320 atacgaatga tgtaaaagat attgtttcaa caaactctag cgcctttaga ttatggtttg 1380 agggcaatta tcagtgtgga tatagagcaa gttatgcaaa ggttcttgat gctgaaacgg 1440 gggaaataaa atgacaaaca aagaaaaaga gttatttgct gaaaatgagg aattaaaaaa 1500 agaaattaag gacttaaaag agcgtattga aagatacaga gaaatggaag ttgaattaag 1560 tacaacaata gatttattga gaggagggat tattgaataa ataaaagccc cctgacgaaa 1620 gtcgaagggg gtttttattt tggtttgatg ttgcgattaa tagatctcgg tgatgacggt 1680 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 1740 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt 1800 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 1860 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac 1920 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga 1980 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa 2040 acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca 2100 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 2160 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 2220 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 2280 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 2340 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 2400 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 2460 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 2520 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 2580 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 2640 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 2700 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg 2760 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 2820 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 2880 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 2940 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 3000 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 3060 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 3120 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 3180 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 3240 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 3300 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 3360 cgtgtagata actagtgttc gtgaatacat gttataataa ctataactaa taacgtaacg 3420 tgactggcaa gagatatttt taaaacaatg aataggttta cacttacttt agttttatgg 3480 aaatgaaaga tcatatcata tataatctag aataaaatta actaaaataa ttattatcta 3540 gataaaaaat ttagaagcca atgaaatcta taaataaact aaattaagtt tatttaatta 3600 acaactatgg atataaaata ggtactaatc aaaatagtga ggaggatata tttgaataca 3660 tacgaacaaa ttaataaagt gaaaaaaata cttcggaaac atttaaaaaa taaccttatt 3720 ggtacttaca tgtttggatc aggagttgag agtggactaa aaccaaatag tgatcttgac 3780 tttttagtcg tcgtatctga accattgaca gatcaaagta aagaaatact tatacaaaaa 3840 attagaccta tttcaaaaaa aataggagat aaaagcaact tacgatatat tgaattaaca 3900 attattattc agcaagaaat ggtaccgtgg aatcatcctc ccaaacaaga atttatttat 3960 ggagaatggt tacaagagct ttatgaacaa ggatacattc ctcagaagga attaaattca 4020 gatttaacca taatgcttta ccaagcaaaa cgaaaaaata aaagaatata cggaaattat 4080 gacttagagg aattactacc tgatattcca ttttctgatg tgagaagagc cattatggat 4140 tcgtcagagg aattaataga taattatcag gatgatgaaa ccaactctat attaacttta 4200 tgccgtatga ttttaactat ggacacgggt aaaatcatac caaaagatat tgcgggaaat 4260 gcagtggctg aatcttctcc attagaacat agggagagaa ttttgttagc agttcgtagt 4320 tatcttggag agaatattga atggactaat gaaaatgtaa atttaactat aaactattta 4380 aataacagat taaaaaaatt ataaaaaaat tgaaaaaatg gtggaaacac ttttttcaat 4440 ttttttgttt tattatttaa tatttgggaa atattcattc taattggtaa tcagatttta 4500 gaaaac 4506 // ID AB055651; SV 1; circular; other DNA; STD; SYN; 4438 BP. XX AC AB055651; XX DT 11-SEP-2001 (Rel. 69, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 3) XX DE Thermosensitive suicide vector pSET5s DNA, complete sequence. XX KW . XX OS Thermosensitive suicide vector pSET5s OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-4438 RA Takamatsu D., Osaki M., Sekizaki T.; RT ; RL Submitted (08-FEB-2001) to the INSDC. RL Daisuke Takamatsu, National Institute of Animal Health, Laboratory of RL Molecular Bacteriology; Kannondai 3-1-1, Tsukuba, Ibaraki 305-0856, Japan RL (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, Fax:81-298-38-7743) XX RN [2] RX DOI; 10.1006/plas.2001.1532. RX PUBMED; 11591139. RA Takamatsu D., Osaki M., Sekizaki T.; RT "Thermosensitive suicide vectors for gene replacement in Streptococcus RT suis"; RL Plasmid 46(2):140-148(2001). XX DR MD5; 3d43b1b07353ff50e269174d6b398def. XX FH Key Location/Qualifiers FH FT source 1..4438 FT /organism="Thermosensitive suicide vector pSET5s" FT /mol_type="other DNA" FT /db_xref="taxon:152115" FT CDS 528..689 FT /codon_start=1 FT /transl_table=11 FT /gene="orfC" FT /product="OrfC" FT /protein_id="BAB64884.1" FT /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA FT RKESEQKK" FT CDS 756..1454 FT /codon_start=1 FT /transl_table=11 FT /gene="repAts" FT /product="RepAts" FT /protein_id="BAB64885.1" FT /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE FT KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD FT YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV FT DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK FT VLDAETGEIK" FT CDS complement(1803..2126) FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="LacZ'" FT /protein_id="BAB64886.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS FT DAA" FT CDS 3593..4243 FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BAB64887.1" FT /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE FT GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV FT KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL FT LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL" XX SQ Sequence 4438 BP; 1370 A; 808 C; 990 G; 1270 T; 0 other; actagttatc ggcataatcg ttaaaacagg cgttatcgta gcgtaaaagc ccttgagcgt 60 agcgtggctt tgcagcgaag atgttgtctg ttagattatg aaagccgatg actgaatgaa 120 ataataagcg cagcgccctt ctatttcggt tggaggaggc tcaagggagt atgagggaat 180 gaaattccct catgggtttg attttaaaaa ttgcttgcaa ttttgccgag cggtagcgct 240 ggaaaatttt tgaaaaaaat ttggaatttg gaaaaaaatg gggggaaagg aagcgaattt 300 tgcttccgta ctacgacccc ccattaagtg ccgagtgcca atttttgtgc caaaaacgct 360 ctatcccaac tggctcaagg gtttaagggg tttttcaatc gccaacgaat cgccaacgtt 420 ttcgccaacg ttttttataa atctatattt aagtagcttt attgttgttt ttatgattac 480 aaagtgatac actaacttta taaaattatt tgattggagt tttttaaatg gtgatttcag 540 aatcgaaaaa aagagttatg atttctctga caaaagagca agataaaaaa ttaacagata 600 tggcgaaaca aaaaggtttt tcaaaatctg cggttgcggc gttagctata gaagaatatg 660 caagaaagga atcagaacaa aaaaaataag cgaaagctcg cgtttttaga aggatacgag 720 ttttcgctac ttgtttttga taaggtaatt atatcatggc tattaaaaat actaaagcta 780 gaaattttgg atttttatta tatcctgact caattcctaa tgattggaaa gaaaaattag 840 agagtttggg cgtatctatg gctgtcagtc ctttacacga tatggacgaa aaaaaagata 900 aagatacatg gaataatagt aatattatac aaaatggaaa gcactataaa aaaccacact 960 atcacgttat atatattgca cgaaatcctg taacaataga aagcgttagg aacaagatta 1020 agcgaaaatt ggggaatagt tcagttgctc atgttgagat acttgattat atcaaaggtt 1080 catatgaata tttgactcat gaatcaaagg acgctattgc taagaataaa catatatacg 1140 acaaaaaaga tattttgaac attaatgatt ttgatattga ccgctatata acacttgatg 1200 aaagccaaaa aagagaattg aagaatttac ttttagatat agtggatgac tataatttgg 1260 taaatacaaa agatttaatg gcttttattc gccttagggg agcggagttt ggaattttaa 1320 atacgaatga tgtaaaagat attgtttcaa caaactctag cgcctttaga ttatggtttg 1380 agggcaatta tcagtgtgga tatagagcaa gttatgcaaa ggttcttgat gctgaaacgg 1440 gggaaataaa atgacaaaca aagaaaaaga gttatttgct gaaaatgagg aattaaaaaa 1500 agaaattaag gacttaaaag agcgtattga aagatacaga gaaatggaag ttgaattaag 1560 tacaacaata gatttattga gaggagggat tattgaataa ataaaagccc cctgacgaaa 1620 gtcgaagggg gtttttattt tggtttgatg ttgcgattaa tagatctcgg tgatgacggt 1680 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 1740 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt 1800 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 1860 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac 1920 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga 1980 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa 2040 acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca 2100 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 2160 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 2220 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 2280 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 2340 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 2400 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 2460 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 2520 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 2580 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 2640 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 2700 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg 2760 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 2820 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 2880 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 2940 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 3000 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 3060 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 3120 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 3180 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 3240 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 3300 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 3360 cgtgtagata actagtcacc gaactagagc ttgatgaaaa tttgtttgat ttttaatgga 3420 taatgtgata taatggttca acaaacgaaa attggataaa gtgggatatt tttaaaatat 3480 atatttatgt tacagtaata ttgactttta aaaaaggatt gattctaatg aagaaagcag 3540 acaagtaagc ctcctaaatt cactttagat aaaaatttag gaggcatatc aaatgaactt 3600 taataaaatt gatttagaca attggaagag aaaagagata tttaatcatt atttgaacca 3660 acaaacgact tttagtataa ccacagaaat tgatattagt gttttatacc gaaacataaa 3720 acaagaagga tataaatttt accctgcatt tattttctta gtgacaaggg tgataaactc 3780 aaatacagct tttagaactg gttacaatag cgacggagag ttaggttatt gggataagtt 3840 agagccactt tatacaattt ttgatggtgt atctaaaaca ttctctggta tttggactcc 3900 tgtaaagaat gacttcaaag agttttatga tttatacctt tctgatgtag agaaatataa 3960 tggttcgggg aaattgtttc ccaaaacacc tatacctgaa aatgcttttt ctctttctat 4020 tattccatgg acttcattta ctgggtttaa cttaaatatc aataataata gtaattacct 4080 tctacccatt attacagcag gaaaattcat taataaaggt aattcaatat atttaccgct 4140 atctttacag gtacatcatt ctgtttgtga tggttatcat gcaggattgt ttatgaactc 4200 tattcaggaa ttgtcagata ggcctaatga ctggctttta taatatgaga taatgccgac 4260 tgtacttttt acagtcggtt ttctaatgtc actaacctgc cccgttagtt gaagaaggtt 4320 tttatattac agctccagat ccatatcctt ctttttctga accgacttct cctttttcgc 4380 ttctttattc caattgcttt attgacgttg agcctcggaa cccatcgaat tatcggaa 4438 // ID AB055652; SV 1; circular; other DNA; STD; SYN; 4438 BP. XX AC AB055652; XX DT 11-SEP-2001 (Rel. 69, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 3) XX DE Thermosensitive suicide vector pSET6s DNA, complete sequence. XX KW . XX OS Thermosensitive suicide vector pSET6s OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-4438 RA Takamatsu D., Osaki M., Sekizaki T.; RT ; RL Submitted (08-FEB-2001) to the INSDC. RL Daisuke Takamatsu, National Institute of Animal Health, Laboratory of RL Molecular Bacteriology; Kannondai 3-1-1, Tsukuba, Ibaraki 305-0856, Japan RL (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, Fax:81-298-38-7743) XX RN [2] RX DOI; 10.1006/plas.2001.1532. RX PUBMED; 11591139. RA Takamatsu D., Osaki M., Sekizaki T.; RT "Thermosensitive suicide vectors for gene replacement in Streptococcus RT suis"; RL Plasmid 46(2):140-148(2001). XX DR MD5; f148f20560fdbb9b3a67bfe39a828142. XX FH Key Location/Qualifiers FH FT source 1..4438 FT /organism="Thermosensitive suicide vector pSET6s" FT /mol_type="other DNA" FT /db_xref="taxon:152116" FT CDS 528..689 FT /codon_start=1 FT /transl_table=11 FT /gene="orfC" FT /product="OrfC" FT /protein_id="BAB64888.1" FT /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA FT RKESEQKK" FT CDS 756..1454 FT /codon_start=1 FT /transl_table=11 FT /gene="repAts" FT /product="RepAts" FT /protein_id="BAB64889.1" FT /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE FT KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD FT YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV FT DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK FT VLDAETGEIK" FT CDS complement(1803..2126) FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ'" FT /product="LacZ'" FT /protein_id="BAB64890.1" FT /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR FT LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS FT DAA" FT CDS complement(3572..4222) FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BAB64891.1" FT /translation="MNFNKIDLDNWKRKEIFNHYLNQQTTFSITTEIDISVLYRNIKQE FT GYKFYPAFIFLVTRVINSNTAFRTGYNSDGELGYWDKLEPLYTIFDGVSKTFSGIWTPV FT KNDFKEFYDLYLSDVEKYNGSGKLFPKTPIPENAFSLSIIPWTSFTGFNLNINNNSNYL FT LPIITAGKFINKGNSIYLPLSLQVHHSVCDGYHAGLFMNSIQELSDRPNDWLL" XX SQ Sequence 4438 BP; 1391 A; 821 C; 977 G; 1249 T; 0 other; actagttatc ggcataatcg ttaaaacagg cgttatcgta gcgtaaaagc ccttgagcgt 60 agcgtggctt tgcagcgaag atgttgtctg ttagattatg aaagccgatg actgaatgaa 120 ataataagcg cagcgccctt ctatttcggt tggaggaggc tcaagggagt atgagggaat 180 gaaattccct catgggtttg attttaaaaa ttgcttgcaa ttttgccgag cggtagcgct 240 ggaaaatttt tgaaaaaaat ttggaatttg gaaaaaaatg gggggaaagg aagcgaattt 300 tgcttccgta ctacgacccc ccattaagtg ccgagtgcca atttttgtgc caaaaacgct 360 ctatcccaac tggctcaagg gtttaagggg tttttcaatc gccaacgaat cgccaacgtt 420 ttcgccaacg ttttttataa atctatattt aagtagcttt attgttgttt ttatgattac 480 aaagtgatac actaacttta taaaattatt tgattggagt tttttaaatg gtgatttcag 540 aatcgaaaaa aagagttatg atttctctga caaaagagca agataaaaaa ttaacagata 600 tggcgaaaca aaaaggtttt tcaaaatctg cggttgcggc gttagctata gaagaatatg 660 caagaaagga atcagaacaa aaaaaataag cgaaagctcg cgtttttaga aggatacgag 720 ttttcgctac ttgtttttga taaggtaatt atatcatggc tattaaaaat actaaagcta 780 gaaattttgg atttttatta tatcctgact caattcctaa tgattggaaa gaaaaattag 840 agagtttggg cgtatctatg gctgtcagtc ctttacacga tatggacgaa aaaaaagata 900 aagatacatg gaataatagt aatattatac aaaatggaaa gcactataaa aaaccacact 960 atcacgttat atatattgca cgaaatcctg taacaataga aagcgttagg aacaagatta 1020 agcgaaaatt ggggaatagt tcagttgctc atgttgagat acttgattat atcaaaggtt 1080 catatgaata tttgactcat gaatcaaagg acgctattgc taagaataaa catatatacg 1140 acaaaaaaga tattttgaac attaatgatt ttgatattga ccgctatata acacttgatg 1200 aaagccaaaa aagagaattg aagaatttac ttttagatat agtggatgac tataatttgg 1260 taaatacaaa agatttaatg gcttttattc gccttagggg agcggagttt ggaattttaa 1320 atacgaatga tgtaaaagat attgtttcaa caaactctag cgcctttaga ttatggtttg 1380 agggcaatta tcagtgtgga tatagagcaa gttatgcaaa ggttcttgat gctgaaacgg 1440 gggaaataaa atgacaaaca aagaaaaaga gttatttgct gaaaatgagg aattaaaaaa 1500 agaaattaag gacttaaaag agcgtattga aagatacaga gaaatggaag ttgaattaag 1560 tacaacaata gatttattga gaggagggat tattgaataa ataaaagccc cctgacgaaa 1620 gtcgaagggg gtttttattt tggtttgatg ttgcgattaa tagatctcgg tgatgacggt 1680 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 1740 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt 1800 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg 1860 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac 1920 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga 1980 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa 2040 acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca 2100 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 2160 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 2220 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 2280 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 2340 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 2400 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 2460 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 2520 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 2580 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 2640 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 2700 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg 2760 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 2820 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 2880 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 2940 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 3000 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 3060 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 3120 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 3180 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 3240 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 3300 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 3360 cgtgtagata actagtttcc gataattcga tgggttccga ggctcaacgt caataaagca 3420 attggaataa agaagcgaaa aaggagaagt cggttcagaa aaagaaggat atggatctgg 3480 agctgtaata taaaaacctt cttcaactaa cggggcaggt tagtgacatt agaaaaccga 3540 ctgtaaaaag tacagtcggc attatctcat attataaaag ccagtcatta ggcctatctg 3600 acaattcctg aatagagttc ataaacaatc ctgcatgata accatcacaa acagaatgat 3660 gtacctgtaa agatagcggt aaatatattg aattaccttt attaatgaat tttcctgctg 3720 taataatggg tagaaggtaa ttactattat tattgatatt taagttaaac ccagtaaatg 3780 aagtccatgg aataatagaa agagaaaaag cattttcagg tataggtgtt ttgggaaaca 3840 atttccccga accattatat ttctctacat cagaaaggta taaatcataa aactctttga 3900 agtcattctt tacaggagtc caaataccag agaatgtttt agatacacca tcaaaaattg 3960 tataaagtgg ctctaactta tcccaataac ctaactctcc gtcgctattg taaccagttc 4020 taaaagctgt atttgagttt atcacccttg tcactaagaa aataaatgca gggtaaaatt 4080 tatatccttc ttgttttatg tttcggtata aaacactaat atcaatttct gtggttatac 4140 taaaagtcgt ttgttggttc aaataatgat taaatatctc ttttctcttc caattgtcta 4200 aatcaatttt attaaagttc atttgatatg cctcctaaat ttttatctaa agtgaattta 4260 ggaggcttac ttgtctgctt tcttcattag aatcaatcct tttttaaaag tcaatattac 4320 tgtaacataa atatatattt taaaaatatc ccactttatc caattttcgt ttgttgaacc 4380 attatatcac attatccatt aaaaatcaaa caaattttca tcaagctcta gttcggtg 4438 // ID AB057644; SV 1; circular; other DNA; STD; SYN; 9071 BP. XX AC AB057644; XX DT 25-MAR-2001 (Rel. 67, Created) DT 25-MAR-2006 (Rel. 87, Last updated, Version 3) XX DE Shuttle vector pVA838 DNA, complete sequence. XX KW . XX OS Shuttle vector pVA838 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-9071 RA Terao Y.; RT ; RL Submitted (16-MAR-2001) to the INSDC. RL Yutaka Terao, Osaka University, Faculty of Dentistry Department of Oral RL Microbiology; Yamadaoka 1-8, Suita, Osaka 565-0871, Japan RL (E-mail:terao@dent.osaka-u.ac.jp, Tel:81-06-6879-2898, Fax:81-06-6878-4755) XX RN [2] RA Terao Y.; RT "Shuttle vector pVA838 complete sequence"; RL Unpublished. XX DR MD5; a7c5102b671884381b0f545b05bb786c. DR EuropePMC; PMC1368962; 16481624. DR EuropePMC; PMC2170534; 17850254. DR EuropePMC; PMC2493227; 18474654. DR EuropePMC; PMC3936844; 24558977. XX FH Key Location/Qualifiers FH FT source 1..9071 FT /organism="Shuttle vector pVA838" FT /lab_host="Escherichia coli" FT /mol_type="other DNA" FT /clone="pVA838" FT /db_xref="taxon:155773" FT CDS 5167..5904 FT /codon_start=1 FT /transl_table=11 FT /gene="erm" FT /product="Erm" FT /protein_id="BAB39762.1" FT /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT FT KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH FT LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH FT PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN FT LSTVTYEQVLSIFNSYLLFNGRK" XX SQ Sequence 9071 BP; 2580 A; 2033 C; 1934 G; 2524 T; 0 other; aagcttatcg atgataagct gtcaaacatg agaattacaa cttatatcgt atggggctga 60 cttcaggtgc tacatttgaa gagataaatt gcactgaaat ctagaaatat tttatctgat 120 taataagatg atcttcttga gatcgttttg gtctgcgcgt aatctcttgc tctgaaaacg 180 aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac tctttgaacc 240 gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt tagccttaac 300 cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc tgccagtggt 360 gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa ggcgcagcgg 420 tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc ctacccggaa 480 ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga caccggtaaa 540 ccgaaaggca ggaacaggag agcgcacgag ggagccgcca ggggaaacgc ctggtatctt 600 tatagtcctg tcgggtttcg ccaccactga tttgagcgtc agatttcgtg atgcttgtca 660 ggggggcgga gcctatggaa aaacggcttt gccgcggccc tctcacttcc ctgttaagta 720 tcttcctggc atcttccagg aaatctccgc cccgttcgta agccatttcc gctcgccgca 780 gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc ggaatatatc ctgtatcaca 840 tattctgctg acgcaccggt gcagcctttt ttctcctgcc acatgaagca cttcactgac 900 accctcatca gtgccaacat agtaagccag tatacactcc gctagcgctg atgtccggcg 960 gtgcttttgc cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa 1020 acagaagcca ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc 1080 atcacccgac gcactttgcg ccgaataaat acctgtgacg gaagatcact tcgcagaata 1140 aataaatcct ggtgtccctg ttgataccgg gaagccctgg gccaactttt ggcgaaaatg 1200 agacgttgat cggcacgtaa gaggttccaa ctttcaccat aatgaaataa gatcactacc 1260 gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa tggagaaaaa 1320 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 1380 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 1440 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 1500 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat 1560 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 1620 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 1680 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 1740 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 1800 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct 1860 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat 1920 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaat ttttttaagg 1980 cagttattgg tgcccttaaa cgcctggtgc tacgcctgaa taagtgataa taagcggatg 2040 aatggcagaa attcgaaagc aaattcgacc cggtcgtcgg ttcagggcag ggtcgttaaa 2100 tagccgctta tgtctattgc tggtttaccg gtttattgac taccggaagc agtgtgaccg 2160 tgtgcttctc aaatgcctga ggccagtttg ctcaggctct ccccgtggag gtaataattg 2220 acgatatgat catttattct gcctcccaga gcctgataaa aacggttagc gcttcgttaa 2280 tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat 2340 aatggtgcag ggcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact 2400 gttgggcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc 2460 ggcgacgata gtcatgcccc gcgcccaccg gaaggagcta ccggacagcg gtgcggactg 2520 ttgtaactca gaataagaaa tgaggccgct catggcgttg actctcagtc atagtatcgt 2580 ggtatcaccg gttggttcca ctctctgttg cgggcaactt cagcagcacg taggggactt 2640 ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt 2700 cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg 2760 ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat 2820 gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat 2880 ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa 2940 gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc 3000 attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg 3060 tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa 3120 atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat 3180 ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg 3240 ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc 3300 gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc 3360 tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc 3420 aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc 3480 tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag 3540 acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg 3600 ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag 3660 cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag 3720 gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa 3780 gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag 3840 gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg 3900 atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa 3960 gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat 4020 agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg 4080 gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca 4140 gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt 4200 gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt gttgtaactg 4260 aaaaaggaaa aattattgtg ccaggcagtt gaaagtcagc accttttaac gagtgctgaa 4320 atgacggcta aatgggaaac gtatttaaaa aaaatcggta aaagagaagg caatcaagag 4380 aactttatta cgaatatcaa aaaattcatt gttcatttac tggaagctgt acctaacgat 4440 atagaaaaac taaatttttc tgattaccag gaacagaaag aaaaagaagc agaaaaaagt 4500 attgtaggaa aatgtcctaa gtgtggcaac aatattgtat taaaaaaatc gttttatggt 4560 tgttcaaatt atcctgaatg taagtttact ttagctgaac attttagaaa gaaaaaactc 4620 accaaaacaa atgtaaaaga attactagag ggaaaagaaa ccctggtaaa aggaatcaaa 4680 acgaaagata gaaagtccta caatgccgtt gtaaaaatcg gagaaaaggg atatattgat 4740 tttatatctt tctcaaaata aacataaaag ccctttaaag agggctttta tatattaatc 4800 acaaatcact tatcacaaat cacaagtgat ttgtgattgt tgatgataaa ataagaataa 4860 gaagaaatag aaagaagtga gtgattgtgg gaaatttagg cgcacaaaaa gaaaaacgaa 4920 atgatacacc aatcagtgca aaaaaagata taatgggaga taagacggtt cgtgttcgtg 4980 ctgacttgca ccatatcata aaaatcgaaa cagcaaagaa tggcggaaac gtaaaagaag 5040 ttatggaaat aagacttaga agcaaactta agagtgtgtt gatagtgcag tatcttaaaa 5100 ttttgtataa taggaattga agttaaatta aatgctaaaa atttgtaatt aagaaggagt 5160 gattacatga acaaaaatat aaaatattct caaaactttt taacgagtga aaaagtactc 5220 aaccaaataa taaaacaatt gaatttaaaa gaaaccgata ccgtttacga aattggaaca 5280 ggtaaagggc atttaacgac gaaactggct aaaataagta aacaggtaac gtctattgaa 5340 ttagacagtc atctattcaa cttatcgtca gaaaaattaa aactgaatac tcgtgtcact 5400 ttaattcacc aagatattct acagtttcaa ttccctaaca aacagaggta taaaattgtt 5460 gggagtattc cttaccattt aagcacacaa attattaaaa aagtggtttt tgaaagccat 5520 gcgtctgaca tctatctgat tgttgaagaa ggattctaca agcgtacctt ggatattcac 5580 cgaacactag ggttgctctt gcacactcaa gtctcgattc agcaattgct taagctgcca 5640 gcggaatgct ttcatcctaa accaaaagta aacagtgtct taataaaact tacccgccat 5700 accacagatg ttccagataa atattggaag ctatatacgt actttgtttc aaaatgggtc 5760 aatcgagaat atcgtcaact gtttactaaa aatcagtttc atcaagcaat gaaacacgcc 5820 aaagtaaaca atttaagtac cgttacttat gagcaagtat tgtctatttt taatagttat 5880 ctattattta acgggaggaa ataattctat gagtcgcttt tgtaaatttg gaaagttaca 5940 cgttactaaa gggaatgtag ataaattatt aggtatacta ctgacagctt ccaaggagct 6000 aaagaggtcc ctagcgccta cggggaattt gtatcgataa ggggtacaaa ttcccactaa 6060 gcgctcgggt ctatatcttt atttgagtgc gtctcaaaaa cacgttcatt atgcttaaaa 6120 gctcctccca aattgccagc tttcatcttc tgcattcttg caaccatgta actcatagat 6180 ttccctcccc aaaaactgtc tttcgattac cgaaagacag acgagccgac acagtctatt 6240 gctcgtctta atcagcaaca tcttaactgg tagtttagca caattcctta tctcacttga 6300 gcttaaagac atattggaaa ctaatgtttt tgtttccgtt ttagtttccg ttttttggtt 6360 tccaatattg ccctcaagta ttgatatgag tactttctcg tccatatcgg aaactacttt 6420 tttattccct ttttttcgac cgagattatt ttgcgataaa atccgattaa gatactgcct 6480 actaactccc aattcttgag ctaattctga tactgtttta ctcatcagct acctcacaaa 6540 ataattcttt ctatcccaat tccaaaaggc aacaatttca cgaccagtac tttctgcaga 6600 ttcttctgct ccggtctgaa caagattccc ttcttcgaca tcatcaagag ctaactccgc 6660 cttgattttc ttgaacaact ttccaaaacc aatctgcctt tttcggtgca aaccattcgt 6720 taaatcttct gttatccgtt ctttgaccat ttcagagaat aatgtcacat cttctgtatc 6780 tacatcaaaa ggcttaacag gatatttagc tgtttcgata atagctgact tcaagttttt 6840 acgtttatga gctttgacgg ttcgaatatc aactacaggt gtataatcca atttcatcgc 6900 cttagcccat agtttcgtcc attcttcttg gctaatgtaa tttgtattat ttccagtaaa 6960 atagctagat ttcacaaata acaatacatg caaatgaggg tgataattct catgctcagt 7020 tgaataagtt acctcagtgg ctcgcaaata gccaataaca tttttatcga ctttcttgta 7080 tttcataaga cgattaaaag ctcgccctat ttctgaaatt gatttattta atttttcacc 7140 actaatgttc ttaatcgtca aggtcaaaaa gagaaagcga ccttttggat agcgtttcat 7200 tgcttcattt accactaatt cagcttgata agcatacttc attgaccgtc tccagttaca 7260 aattgggcag agcttatttt tgcaaaaata agactgatag agttttttcg tgccgtcttg 7320 ttgttcgata aacttcaaca cttcggcaca ttgatagacc cgttcaaacg aacgatatcc 7380 taaacgctct aaacgaccag caagctcaat attttttaac ttgcgttctc gccactttct 7440 atctttgcca ttcttagaat agtccttaaa gacttgattt tcagtacctg tcatgttata 7500 attttcttgc aaaaaaattt ttttgagtat ataaaaaagt tcctgcaatt ctcctaagtt 7560 tattatttta aattggacac ttaaattata aactttattt tattaaattg caagggcttt 7620 tttgttacct aaaaacccag caatatcaag ggtttgaggt gctttaaaac aagaaaataa 7680 aaaaactcat tatttctata tgtatcaaga taagaaaaaa cccttgctac gcatgggttt 7740 ttaagggctt ttcagccctc tttttcgttt cacgaaaaat tcacccctaa aacctcaaat 7800 tttgactttc taaatccaaa cgtggtataa taattttaac gctagaagtt ctaggaaacg 7860 aatagcacaa cgcaaaaaaa gacagtagct ggaactactg tctttctttt ttgtcctcaa 7920 aaagaactca ggttaatttt tggtcgcgtt tgtttcgaac ataccgaatc aaccaatcag 7980 cacttacctc tgcagcaacg ccaaccacaa aaggtatcaa aacaagttct aggaaagtct 8040 tcacagcgct cacctccaat ctaagttcgt aacttagcga aagaacgttg cgacctagct 8100 attataccat gtccgcttac tttattccat ggtctaattg acaacaagta accaagggcg 8160 gacgtccttt gtccgtgtcg gctcgaaacg ctaaagcctt tcggctcgtc acgtcctagc 8220 gtactttgcc cttgcttatt gtcaattagc tttcacggca taaatcgctc aaaggcctag 8280 ccctttttca atcactcgtt taactaccct tacgattggc tgaatagctc ttaactctga 8340 tataaaattt tttaaggctt tatctggcat aaactctttc agttttccta gcattttgtc 8400 attttgctgt ctcaaaactg agttttcact tctaagcgct ctattttcgt tcctaagacg 8460 ctctatgctc tctcccttac ttttttcttt agctcgttcc aaactcgctt caaaaccgtt 8520 tttagagcgt ctcagaggca ctatccgcct taattgctca ttctctagct ctttttgatg 8580 aacaaggttt ctgcttcggt atatcagccc tttaaattct tcaaactgct ctttagtcaa 8640 ttccacatat tctttaccga acacgccttt tttaggggta atagccgcta gttctttctc 8700 agacatgatt tttaggtcag ctatttgtgt ttttagtttt tctaatttca gcccctcgcg 8760 ctctaaacgc tctattttcg cttctaagct cttcgaataa ttttctaagg tatttatata 8820 tccctcagct ttagatagct ctgagagcct ctcagaggtc tcagaatcga ttttaatata 8880 ttccttggtc ttttcatcaa gcaactcttc taaatggctt aaatcgtctt ctaggggctt 8940 tttagacgat tctagttttt tcagctcttc ttggtactgt tttcttaacc agtccatttt 9000 ttctcggtta gttgtttcac gtatcggaga ttgaacttca aacatatcag caaactcttt 9060 ttcatgccaa a 9071 // ID AB058940; SV 1; linear; other DNA; STD; SYN; 45 BP. XX AC AB058940; XX DT 02-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 3) XX DE Synthetic construct gene for peptide mimetic of IFN beta, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-45 RA Sato A., Kawaguchi K., Kimura K., Tanimura R., Sone S.; RT ; RL Submitted (30-MAR-2001) to the INSDC. RL Atsushi Sato, Toray Industries Inc., Pharmaceutical Research Laboratory; RL 1111,tebiro, kamakura, Kanagawa 248-8555, Japan RL (E-mail:satou-a@blab.toray.co.jp, Tel:81-467-32-9927, Fax:81-467-32-2135) XX RN [2] RX DOI; 10.1042/BJ20020993. RX PUBMED; 12542398. RA Sato A., Sone S.; RT "A peptide mimetic of human interferon (IFN)-beta"; RL Biochem. J. 371(Pt 2):603-608(2003). XX DR MD5; 87ca57d1535941b2a26dfce285682778. XX FH Key Location/Qualifiers FH FT source 1..45 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /clone="6" FT /db_xref="taxon:32630" FT CDS <1..>45 FT /codon_start=1 FT /transl_table=11 FT /product="peptide mimetic of IFN beta" FT /note="SYR6" FT /protein_id="BAB62415.1" FT /translation="SVQARWEAAFDLDLY" XX SQ Sequence 45 BP; 7 A; 6 C; 15 G; 17 T; 0 other; agtgttcagg ctaggtggga ggctgctttt gatctggatc tttat 45 // ID AB061673; SV 1; linear; other DNA; STD; SYN; 2151 BP. XX AC AB061673; XX DT 04-JUL-2001 (Rel. 68, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 5) XX DE Synthetic construct gene for Picchu-236, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Homo sapiens (human) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. XX RN [1] RP 1-2151 RA Matsuda M.; RT ; RL Submitted (11-MAY-2001) to the INSDC. RL Michiyuki Matsuda, Institute for Microbial Diseases, Osaka University, RL Department of Tumor Virology; Yamadaoka 3-1, Suita, Osaka 565-0871, Japan RL (E-mail:matsudam@biken.osaka-u.ac.jp, URL:www-tv.biken.osaka-u.ac.jp, RL Tel:81-6-6879-8316, Fax:81-6879-8314) XX RN [2] RX DOI; 10.1074/jbc.M104341200. RX PUBMED; 11406630. RA Kurokawa K., Mochizuki N., Ohba Y., Mizuno H., Miyawaki A., Matsuda M.; RT "A pair of fluorescent resonance energy transfer-based probes for tyrosine RT phosphorylation of the CrkII adaptor protein in vivo"; RL J Biol Chem 276(33):31305-31310(2001). XX DR MD5; cd55b9005f7364f95fe827f219d22927. XX FH Key Location/Qualifiers FH FT source 1..2151 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT source 724..1428 FT /organism="Homo sapiens" FT /mol_type="other DNA" FT /note="human CrkII" FT /db_xref="taxon:9606" FT CDS 1..2151 FT /codon_start=1 FT /transl_table=11 FT /gene="Picchu-236" FT /product="Picchu-236" FT /protein_id="BAB61871.1" FT /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL FT KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD FT GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK FT VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL FT EFVTAAGITLGMDELYKLDMAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDS FT STSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPA FT LLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDI FT LRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPE FT PGPYAQPSVNTPLPNLQNGGRMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA FT TYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQER FT TIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITAD FT KQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEK FT RDHMVLLEFVTAAGITLGMDEL" FT misc_feature 1..717 FT /gene="EYFP" FT misc_binding 1384..1386 FT /bound_moiety="phosphotyrosine" FT misc_feature 1438..2151 FT /gene="ECFP" XX SQ Sequence 2151 BP; 501 A; 682 C; 626 G; 342 T; 0 other; atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag 240 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagctc 720 gacatggcgg gcaacttcga ctcggaggag cggagtagct ggtactgggg gcggttgagt 780 cggcaggagg cggtggcgct gctgcagggc cagcggcacg gggtgttcct ggtgcgggac 840 tcgagcacca gccccgggga ctatgtgctc agcgtctccg aaaactcgcg cgtctcccac 900 tacatcatca acagcagcgg cccgcgccct ccagtgcctc cgtcgcccgc tcagcctccg 960 ccgggagtga gtccctccag gctccgaata ggagatcaag aatttgattc attgcctgct 1020 ttactggaat tctacaaaat acactatttg gacactacaa cactgataga accagtttcc 1080 agatcaaggc agggtagtgg agtgattctc aggcaggagg aggcagagta tgtgcgggcc 1140 ctctttgact ttaatgggaa tgatgaagaa gatcttccct ttaagaaagg agacatcctg 1200 agaatccggg ataagcctga agagcagtgg tggaatgcag aggacagcga aggaaagagg 1260 gggatgattc ctgtccctta cgtggagaag tatagacccg cctccgcctc agtatcggct 1320 ctgattggag gtaaccagga gggttcccac ccacagccac tgggtgggcc ggagcctggg 1380 ccctatgccc aacccagcgt caacactccg ctccctaacc tccagaatgg cggccgcatg 1440 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 1500 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 1560 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 1620 gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag 1680 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 1740 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 1800 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 1860 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc 1920 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 1980 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 2040 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 2100 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta g 2151 // ID AB061674; SV 1; linear; other DNA; STD; SYN; 2223 BP. XX AC AB061674; XX DT 04-JUL-2001 (Rel. 68, Created) DT 24-JAN-2004 (Rel. 78, Last updated, Version 4) XX DE Synthetic construct gene for Picchu-236-X, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Homo sapiens (human) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; OC Homo. XX RN [1] RP 1-2223 RA Matsuda M.; RT ; RL Submitted (11-MAY-2001) to the INSDC. RL Michiyuki Matsuda, Institute for Microbial Diseases, Osaka University, RL Department of Tumor Virology; Yamadaoka 3-1, Suita, Osaka 565-0871, Japan RL (E-mail:matsudam@biken.osaka-u.ac.jp, URL:www-tv.biken.osaka-u.ac.jp, RL Tel:81-6-6879-8316, Fax:81-6879-8314) XX RN [2] RX DOI; 10.1074/jbc.M104341200. RX PUBMED; 11406630. RA Kurokawa K., Mochizuki N., Ohba Y., Mizuno H., Miyawaki A., Matsuda M.; RT "A pair of fluorescent resonance energy transfer-based probes for tyrosine RT phosphorylation of the CrkII adaptor protein in vivo"; RL J Biol Chem 276(33):31305-31310(2001). XX DR MD5; 72782073015ef06cd738ae09a4c358e8. XX FH Key Location/Qualifiers FH FT source 1..2223 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT source 724..1428 FT /organism="Homo sapiens" FT /mol_type="other DNA" FT /note="human CrkII" FT /db_xref="taxon:9606" FT CDS 1..2223 FT /codon_start=1 FT /transl_table=11 FT /gene="Picchu-236-X" FT /product="Picchu-236-X" FT /protein_id="BAB61872.1" FT /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL FT KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD FT GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK FT VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL FT EFVTAAGITLGMDELYKLDMAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDS FT STSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPA FT LLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDI FT LRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPE FT PGPYAQPSVNTPLPNLQNGGRMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDA FT TYGKLTLKFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQER FT TIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITAD FT KQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEK FT RDHMVLLEFVTAAGITLGMDELGRSRKMSKDGKKKKKKSKTKCVIM" FT misc_feature 1..717 FT /gene="EYFP" FT misc_binding 1384..1386 FT /bound_moiety="phosphotyrosine" FT misc_feature 1438..2151 FT /gene="ECFP" FT regulatory 2161..2223 FT /function="CAAX box" FT /regulatory_class="other" XX SQ Sequence 2223 BP; 536 A; 689 C; 644 G; 354 T; 0 other; atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac 60 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac 120 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc 180 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag 240 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc 300 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg 360 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac 420 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac 480 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc 540 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac 600 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc 660 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagctc 720 gacatggcgg gcaacttcga ctcggaggag cggagtagct ggtactgggg gcggttgagt 780 cggcaggagg cggtggcgct gctgcagggc cagcggcacg gggtgttcct ggtgcgggac 840 tcgagcacca gccccgggga ctatgtgctc agcgtctccg aaaactcgcg cgtctcccac 900 tacatcatca acagcagcgg cccgcgccct ccagtgcctc cgtcgcccgc tcagcctccg 960 ccgggagtga gtccctccag gctccgaata ggagatcaag aatttgattc attgcctgct 1020 ttactggaat tctacaaaat acactatttg gacactacaa cactgataga accagtttcc 1080 agatcaaggc agggtagtgg agtgattctc aggcaggagg aggcagagta tgtgcgggcc 1140 ctctttgact ttaatgggaa tgatgaagaa gatcttccct ttaagaaagg agacatcctg 1200 agaatccggg ataagcctga agagcagtgg tggaatgcag aggacagcga aggaaagagg 1260 gggatgattc ctgtccctta cgtggagaag tatagacccg cctccgcctc agtatcggct 1320 ctgattggag gtaaccagga gggttcccac ccacagccac tgggtgggcc ggagcctggg 1380 ccctatgccc aacccagcgt caacactccg ctccctaacc tccagaatgg cggccgcatg 1440 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc 1500 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc 1560 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc 1620 gtgaccaccc tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag 1680 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc 1740 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg 1800 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag 1860 ctggagtaca actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc 1920 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac 1980 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac 2040 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg 2100 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctggg ccgctctaga 2160 aagatgagca aagatggtaa aaagaagaaa aagaagtcaa agacaaagtg tgtaattatg 2220 taa 2223 // ID AB062118; SV 1; linear; other DNA; STD; SYN; 1533 BP. XX AC AB062118; XX DT 23-NOV-2001 (Rel. 69, Created) DT 01-JUN-2003 (Rel. 76, Last updated, Version 3) XX DE Synthetic construct Danio rerio HSF gene for heat shock transcription DE factor with His-tag, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1533 RA Yamashita M.; RT ; RL Submitted (22-MAY-2001) to the INSDC. RL Michiaki Yamashita, National Research Institute of Fisheries Science; RL Fukuura-2, Yokohama, Kanagawa 236-8648, Japan (E-mail:mic@affrc.go.jp, RL Tel:81-45-788-7665, Fax:81-45-788-5001) XX RN [2] RA Yamashita M.; RT "Stress protein (heat shock protein) HSP70 and heat shock transcription RT factor HSF in zebrafish"; RL Unpublished. XX DR MD5; b40b1947e34f066bf7eeaa2e0363d6cc. XX FH Key Location/Qualifiers FH FT source 1..1533 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="Danio rerio (zebrafish) HSF cDNA with His-tag at FT C-terminal" FT /db_xref="taxon:32630" FT CDS 14..1525 FT /codon_start=1 FT /transl_table=11 FT /gene="HSF" FT /product="heat shock transcription factor with His-tag" FT /protein_id="BAB72172.1" FT /translation="MEYHSVGPGGVVVTGNNVPAFLTKLWTLVEDPDTDPLICWSPNGT FT SFHVFDQGRFSKEVLPKYFKHNNMASFVRQLNMYGFRKVVHIEQGGLVKPEKDDTEFQH FT PYFIRGQEQLLENIKRKVTTVSNIKHEDYKFSTDDVSKMISDVQHMKGKQESMDSKIST FT LKHENEMLWREVATLRQKHSQQQKVVNKLIQFLITLARSNRVLGVKRKMPLMLNDSSSA FT HSMPKFSRQYSLESPAPSSTAFTGTGVFSPEPPVKTGPIISDITELAQSSPVATDEWIE FT DRTSPLVHIKEEPSSPAHSPEVEEVCPVEVEVGAGSDLPVDTPLSPTTFINSILQESEP FT VSALTPPPSEQKCLSVACLDKTELHDHLESIDSGLENLQQILNAQSINFDSSPLFDIFS FT SAASDVGLDSLASIQDLLSPDPVKETESGLDTDSGKQLVQYTSQPIVLPDPLSTDSSST FT DLPMLLELQDDSYFSSEPAEDPTIALLNLQPVPEDPKLSHHHHHH" XX SQ Sequence 1533 BP; 392 A; 453 C; 373 G; 315 T; 0 other; tctagaattc catatggagt atcacagtgt ggggcccgga ggagtggtgg tcaccgggaa 60 caatgtgcct gcgtttctga ccaaactctg gacgctggtg gaggatccgg acacggaccc 120 gctgatctgc tggagcccaa atggcaccag tttccacgtc ttcgaccagg gcaggttttc 180 caaagaagtt ctgccgaaat acttcaagca caacaacatg gccagttttg tccgccagct 240 caatatgtat ggttttcgga aagtggtcca cattgagcag ggagggctgg taaaaccaga 300 gaaagacgac acagagttcc agcatccata tttcatccgg ggacaggagc aacttctgga 360 gaatattaag aggaaggtga ccacggtgtc gaatattaaa catgaggact acaagttcag 420 cacggacgac gtgtctaaaa tgatctcaga tgtccagcac atgaagggca agcaggagtc 480 catggactcc aaaatcagca ccctcaagca tgagaacgag atgctctgga gggaagttgc 540 cactttgaga cagaaacact ctcaacaaca gaaagtcgtc aataagctta ttcagttcct 600 gatcacatta gcacggtcca acagagtcct gggagtgaaa cgaaagatgc cgctgatgtt 660 gaacgacagc agctctgctc actccatgcc caaattctct cgtcagtact cgctggagtc 720 tcctgctccc tccagcacag ctttcacagg aactggcgtc ttcagcccag aaccgccagt 780 caagacgggc ccaatcatct ctgacatcac tgaactggcc cagtccagcc ctgtggccac 840 agacgagtgg attgaagacc gaacgagccc tttggtccac atcaaagaag agccgtctag 900 ccccgcccac agccctgagg tggaagaggt gtgtcctgtg gaagtagaag tgggggctgg 960 gtctgatttg cctgtggaca cgcccctttc gcccacaacc ttcataaact ccatccttca 1020 ggagagcgag ccagtctccg ccctgactcc gcctcccagc gaacagaagt gcttgagcgt 1080 cgcctgcctg gacaagacgg agcttcatga ccatctggag tccatcgaca gcggtttaga 1140 aaacctccag cagatcctca acgcgcagtc cattaacttc gactcctctc ctctcttcga 1200 cattttctct tcagcggcgt ctgatgtggg tctggacagc ctggctagta tccaagatct 1260 tttgtcacca gatccagtga aagaaaccga atctggtctg gacactgatt caggcaagca 1320 gctggtccag tacacgtcgc agcccatcgt tctccctgat cccctcagca ccgacagcag 1380 cagcacagat ctgcccatgc tcctggagct ccaggatgac tcgtatttca gctctgaacc 1440 cgccgaagat cccaccatcg ccctgctcaa cctccagcct gttcccgaag accccaaact 1500 atcacaccac caccaccacc actaagcggc cgc 1533 // ID AB062119; SV 1; linear; other DNA; STD; SYN; 1041 BP. XX AC AB062119; XX DT 23-NOV-2001 (Rel. 69, Created) DT 01-JUN-2003 (Rel. 76, Last updated, Version 3) XX DE Synthetic construct Danio rerio HSF gene for heat shock transcription DE factor mutant, truncated from Ser-236 to Ala-402, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1041 RA Yamashita M.; RT ; RL Submitted (22-MAY-2001) to the INSDC. RL Michiaki Yamashita, National Research Institute of Fisheries Science; RL Fukuura-2, Yokohama, Kanagawa 236-8648, Japan (E-mail:mic@affrc.go.jp, RL Tel:81-45-788-7665, Fax:81-45-788-5001) XX RN [2] RA Yamashita M.; RT "Stress protein (heat shock protein) HSP70 and heat shock transcription RT factor HSF in zebrafish"; RL Unpublished. XX DR MD5; 036fbd49c0d890b6eddddb4eaab0ed3e. XX FH Key Location/Qualifiers FH FT source 1..1041 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="Danio rerio (zebrafish) HSF cDNA with His-tag at FT C-terminal" FT /db_xref="taxon:32630" FT CDS 14..1033 FT /codon_start=1 FT /transl_table=11 FT /gene="HSF" FT /product="heat shock transcription factor mutant" FT /note="zebrafish heat shock transcription factor HSF mutant FT truncated from Ser-236 to Ala-402" FT /protein_id="BAB72173.1" FT /translation="MEYHSVGPGGVVVTGNNVPAFLTKLWTLVEDPDTDPLICWSPNGT FT SFHVFDQGRFSKEVLPKYFKHNNMASFVRQLNMYGFRKVVHIEQGGLVKPEKDDTEFQH FT PYFIRGQEQLLENIKRKVTTVSNIKHEDYKFSTDDVSKMISDVQHMKGKQESMDSKIST FT LKHENEMLWREVATLRQKHSQQQKVVNKLIQFLITLARSNRVLGVKRKMPLMLNDSSSA FT HSMPKFSRQYSLELVASDVGLDSLASIQDLLSPDPVKETESGLDTDSGKQLVQYTSQPI FT VLPDPLSTDSSSTDLPMLLELQDDSYFSSEPAEDPTIALLNLQPVPEDPKLSHHHHHH" XX SQ Sequence 1041 BP; 284 A; 288 C; 255 G; 214 T; 0 other; tctagaattc catatggagt atcacagtgt ggggcccgga ggagtggtgg tcaccgggaa 60 caatgtgcct gcgtttctga ccaaactctg gacgctggtg gaggatccgg acacggaccc 120 gctgatctgc tggagcccaa atggcaccag tttccacgtc ttcgaccagg gcaggttttc 180 caaagaagtt ctgccgaaat acttcaagca caacaacatg gccagttttg tccgccagct 240 caatatgtat ggttttcgga aagtggtcca cattgagcag ggagggctgg taaaaccaga 300 gaaagacgac acagagttcc agcatccata tttcatccgg ggacaggagc aacttctgga 360 gaatattaag aggaaggtga ccacggtgtc gaatattaaa catgaggact acaagttcag 420 cacggacgac gtgtctaaaa tgatctcaga tgtccagcac atgaagggca agcaggagtc 480 catggactcc aaaatcagca ccctcaagca tgagaacgag atgctctgga gggaagttgc 540 cactttgaga cagaaacact ctcaacaaca gaaagtcgtc aataagctta ttcagttcct 600 gatcacatta gcacggtcca acagagtcct gggagtgaaa cgaaagatgc cgctgatgtt 660 gaacgacagc agctctgctc actccatgcc caaattctct cgtcagtact cgctggaact 720 agtggcgtct gatgtgggtc tggacagcct ggctagtatc caagatcttt tgtcaccaga 780 tccagtgaaa gaaaccgaat ctggtctgga cactgattca ggcaagcagc tggtccagta 840 cacgtcgcag cccatcgttc tccctgatcc cctcagcacc gacagcagca gcacagatct 900 gcccatgctc ctggagctcc aggatgactc gtatttcagc tctgaacccg ccgaagatcc 960 caccatcgcc ctgctcaacc tccagcctgt tcccgaagac cccaaactat cacaccacca 1020 ccaccaccac taagcggccg c 1041 // ID AB062120; SV 1; linear; other DNA; STD; SYN; 1386 BP. XX AC AB062120; XX DT 23-NOV-2001 (Rel. 69, Created) DT 01-JUN-2003 (Rel. 76, Last updated, Version 3) XX DE Synthetic construct Danio rerio HSF gene for heat shock transcription DE factor mutant, truncated from Gln-351 to 402, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1386 RA Yamashita M.; RT ; RL Submitted (22-MAY-2001) to the INSDC. RL Michiaki Yamashita, National Research Institute of Fisheries Science; RL Fukuura-2, Yokohama, Kanagawa 236-8648, Japan (E-mail:mic@affrc.go.jp, RL Tel:81-45-788-7665, Fax:81-45-788-5001) XX RN [2] RA Yamashita M.; RT "Stress protein (heat shock protein) HSP70 and heat shock transcription RT factor HSF in zebrafish"; RL Unpublished. XX DR MD5; ca0913e4d41dfc7ae61f1aaaeff6c52a. XX FH Key Location/Qualifiers FH FT source 1..1386 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="Danio rerio (zebrafish) HSF cDNA with His-tag at FT C-terminal" FT /db_xref="taxon:32630" FT CDS 14..1378 FT /codon_start=1 FT /transl_table=11 FT /gene="HSF" FT /product="heat shock transcription factor mutant" FT /note="zebrafish heat shock transcription factor HSF mutant FT truncated from Gln-351 to 402" FT /protein_id="BAB72174.1" FT /translation="MEYHSVGPGGVVVTGNNVPAFLTKLWTLVEDPDTDPLICWSPNGT FT SFHVFDQGRFSKEVLPKYFKHNNMASFVRQLNMYGFRKVVHIEQGGLVKPEKDDTEFQH FT PYFIRGQEQLLENIKRKVTTVSNIKHEDYKFSTDDVSKMISDVQHMKGKQESMDSKIST FT LKHENEMLWREVATLRQKHSQQQKVVNKLIQFLITLARSNRVLGVKRKMPLMLNDSSSA FT HSMPKFSRQYSLESPAPSSTAFTGTGVFSPEPPVKTGPIISDITELAQSSPVATDEWIE FT DRTSPLVHIKEEPSSPAHSPEVEEVCPVEVEVGAGSDLPVDTPLSPTTFINSILQESEP FT VSALTPPPSELVASDVGLDSLASIQDLLSPDPVKETESGLDTDSGKQLVQYTSQPIVLP FT DPLSTDSSSTDLPMLLELQDDSYFSSEPAEDPTIALLNLQPVPEDPKLSHHHHHH" XX SQ Sequence 1386 BP; 359 A; 405 C; 343 G; 279 T; 0 other; tctagaattc catatggagt atcacagtgt ggggcccgga ggagtggtgg tcaccgggaa 60 caatgtgcct gcgtttctga ccaaactctg gacgctggtg gaggatccgg acacggaccc 120 gctgatctgc tggagcccaa atggcaccag tttccacgtc ttcgaccagg gcaggttttc 180 caaagaagtt ctgccgaaat acttcaagca caacaacatg gccagttttg tccgccagct 240 caatatgtat ggttttcgga aagtggtcca cattgagcag ggagggctgg taaaaccaga 300 gaaagacgac acagagttcc agcatccata tttcatccgg ggacaggagc aacttctgga 360 gaatattaag aggaaggtga ccacggtgtc gaatattaaa catgaggact acaagttcag 420 cacggacgac gtgtctaaaa tgatctcaga tgtccagcac atgaagggca agcaggagtc 480 catggactcc aaaatcagca ccctcaagca tgagaacgag atgctctgga gggaagttgc 540 cactttgaga cagaaacact ctcaacaaca gaaagtcgtc aataagctta ttcagttcct 600 gatcacatta gcacggtcca acagagtcct gggagtgaaa cgaaagatgc cgctgatgtt 660 gaacgacagc agctctgctc actccatgcc caaattctct cgtcagtact cgctggagtc 720 tcctgctccc tccagcacag ctttcacagg aactggcgtc ttcagcccag aaccgccagt 780 caagacgggc ccaatcatct ctgacatcac tgaactggcc cagtccagcc ctgtggccac 840 agacgagtgg attgaagacc gaacgagccc tttggtccac atcaaagaag agccgtctag 900 ccccgcccac agccctgagg tggaagaggt gtgtcctgtg gaagtagaag tgggggctgg 960 gtctgatttg cctgtggaca cgcccctttc gcccacaacc ttcataaact ccatccttca 1020 ggagagcgag ccagtctccg ccctgactcc gcctcccagc gaactagtgg cgtctgatgt 1080 gggtctggac agcctggcta gtatccaaga tcttttgtca ccagatccag tgaaagaaac 1140 cgaatctggt ctggacactg attcaggcaa gcagctggtc cagtacacgt cgcagcccat 1200 cgttctccct gatcccctca gcaccgacag cagcagcaca gatctgccca tgctcctgga 1260 gctccaggat gactcgtatt tcagctctga acccgccgaa gatcccacca tcgccctgct 1320 caacctccag cctgttcccg aagaccccaa actatcacac caccaccacc accactaagc 1380 ggccgc 1386 // ID AB062167; SV 1; linear; other DNA; STD; SYN; 6812 BP. XX AC AB062167; XX DT 28-NOV-2001 (Rel. 69, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Cloning vector pRTHSP70-lacZ gene for beta-galactosidase, promoter region DE and complete cds. XX KW . XX OS Cloning vector pRTHSP70-lacZ OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-6812 RA Yamashita M., Ojima N.; RT ; RL Submitted (23-MAY-2001) to the INSDC. RL Michiaki Yamashita, National Research Institute of Fisheries Science; RL Fukuura-2, Yokohama, Kanagawa 236-8648, Japan (E-mail:mic@affrc.go.jp, RL Tel:81-45-788-7665, Fax:81-45-788-5001) XX RN [2] RA Yamashita M., Ojima N.; RT "Transgenic zebrafish as a biosensor for enviromental stress"; RL Unpublished. XX DR MD5; 910b8625eac99d23ac5cbccc34366fe6. XX FH Key Location/Qualifiers FH FT source 1..6812 FT /organism="Cloning vector pRTHSP70-lacZ" FT /mol_type="other DNA" FT /db_xref="taxon:160966" FT regulatory 61..200 FT /gene="HSP70" FT /function="heat shock promoter" FT /note="rainbow trout HSP70" FT /regulatory_class="promoter" FT CDS 326..3553 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ" FT /product="beta-galactosidase" FT /protein_id="BAB72231.1" FT /translation="MLQIHARKLASRLMPSEQWKGIIAVSRGGLVPVGEDQKQHLELSR FT DIAQRFNALYGEIDPVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLR FT SLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNP FT PFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSE FT FDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVAT FT RFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADR FT VTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGK FT PLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYG FT LYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHG FT ANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWL FT SLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGN FT PWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEY FT LFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVV FT QPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNR FT QSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAE FT AALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPH FT PARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGL FT RCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGD FT DSWSPSVSAEFQLSAGRYHYQLVWCQK" FT regulatory 3560..3947 FT /note="SV40 late polyadenylation signal" FT /regulatory_class="other" XX SQ Sequence 6812 BP; 1622 A; 1768 C; 1718 G; 1704 T; 0 other; cccgggaggt accgagctct tacgcgtgct agctcgagat cccccgggct gcaggaattc 60 gattggttgt tattcaagaa tattcaggaa aacatctctg ttggttgaga ttgacattac 120 ttgcacctag gttttcatag aataatatca atttctaaaa tatttcatta ttttgtaggc 180 aactaactcc aaacctacaa tctagaatca agcttgggat ctctataatc tcgcgcaacc 240 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag 300 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg 360 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctggtacc 420 ggtgggtgaa gaccagaaac agcacctcga actgagccgc gatattgccc agcgtttcaa 480 cgcgctgtat ggcgagatcg atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg 540 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 600 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt 660 tgcctggttt ccggcaccag aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga 720 ggccgatact gtcgtcgtcc cctcaaactg gcagatgcac ggttacgatg cgcccatcta 780 caccaacgtg acctatccca ttacggtcaa tccgccgttt gttcccacgg agaatccgac 840 gggttgttac tcgctcacat ttaatgttga tgaaagctgg ctacaggaag gccagacgcg 900 aattattttt gatggcgtta actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg 960 ttacggccag gacagtcgtt tgccgtctga atttgacctg agcgcatttt tacgcgccgg 1020 agaaaaccgc ctcgcggtga tggtgctgcg ctggagtgac ggcagttatc tggaagatca 1080 ggatatgtgg cggatgagcg gcattttccg tgacgtctcg ttgctgcata aaccgactac 1140 acaaatcagc gatttccatg ttgccactcg ctttaatgat gatttcagcc gcgctgtact 1200 ggaggctgaa gttcagatgt gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt 1260 atggcagggt gaaacgcagg tcgccagcgg caccgcgcct ttcggcggtg aaattatcga 1320 tgagcgtggt ggttatgccg atcgcgtcac actacgtctg aacgtcgaaa acccgaaact 1380 gtggagcgcc gaaatcccga atctctatcg tgcggtggtt gaactgcaca ccgccgacgg 1440 cacgctgatt gaagcagaag cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg 1500 tctgctgctg ctgaacggca agccgttgct gattcgaggc gttaaccgtc acgagcatca 1560 tcctctgcat ggtcaggtca tggatgagca gacgatggtg caggatatcc tgctgatgaa 1620 gcagaacaac tttaacgccg tgcgctgttc gcattatccg aaccatccgc tgtggtacac 1680 gctgtgcgac cgctacggcc tgtatgtggt ggatgaagcc aatattgaaa cccacggcat 1740 ggtgccaatg aatcgtctga ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt 1800 aacgcgaatg gtgcagcgcg atcgtaatca cccgagtgtg atcatctggt cgctggggaa 1860 tgaatcaggc cacggcgcta atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc 1920 ttcccgcccg gtgcagtatg aaggcggcgg agccgacacc acggccaccg atattatttg 1980 cccgatgtac gcgcgcgtgg atgaagacca gcccttcccg gctgtgccga aatggtccat 2040 caaaaaatgg ctttcgctac ctggagagac gcgcccgctg atcctttgcg aatacgccca 2100 cgcgatgggt aacagtcttg gcggtttcgc taaatactgg caggcgtttc gtcagtatcc 2160 ccgtttacag ggcggcttcg tctgggactg ggtggatcag tcgctgatta aatatgatga 2220 aaacggcaac ccgtggtcgg cttacggcgg tgattttggc gatacgccga acgatcgcca 2280 gttctgtatg aacggtctgg tctttgccga ccgcacgccg catccagcgc tgacggaagc 2340 aaaacaccag cagcagtttt tccagttccg tttatccggg caaaccatcg aagtgaccag 2400 cgaatacctg ttccgtcata gcgataacga gctcctgcac tggatggtgg cgctggatgg 2460 taagccgctg gcaagcggtg aagtgcctct ggatgtcgct ccacaaggta aacagttgat 2520 tgaactgcct gaactaccgc agccggagag cgccgggcaa ctctggctca cagtacgcgt 2580 agtgcaaccg aacgcgaccg catggtcaga agccgggcac atcagcgcct ggcagcagtg 2640 gcgtctggcg gaaaacctca gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct 2700 gaccaccagc gaaatggatt tttgcatcga gctgggtaat aagcgttggc aatttaaccg 2760 ccagtcaggc tttctttcac agatgtggat tggcgataaa aaacaactgc tgacgccgct 2820 gcgcgatcag ttcacccgtg caccgctgga taacgacatt ggcgtaagtg aagcgacccg 2880 cattgaccct aacgcctggg tcgaacgctg gaaggcggcg ggccattacc aggccgaagc 2940 agcgttgttg cagtgcacgg cagatacact tgctgatgcg gtgctgatta cgaccgctca 3000 cgcgtggcag catcagggga aaaccttatt tatcagccgg aaaacctacc ggattgatgg 3060 tagtggtcaa atggcgatta ccgttgatgt tgaagtggcg agcgatacac cgcatccggc 3120 gcggattggc ctgaactgcc agctggcgca ggtagcagag cgggtaaact ggctcggatt 3180 agggccgcaa gaaaactatc ccgaccgcct tactgccgcc tgttttgacc gctgggatct 3240 gccattgtca gacatgtata ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg 3300 gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt tcaacatcag 3360 ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc acgcggaaga 3420 aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg actcctggag 3480 cccgtcagta tcggcggaat tccagctgag cgccggtcgc taccattacc agttggtctg 3540 gtgtcaaaaa taataataac cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc 3600 cattatgtac tatttaaaaa acacaaactt ttggatgttc ggtttattct ttttctttta 3660 cttttttatc atgggagcct acttcccgtt tttcccgatt tggctacatg acatcaacca 3720 tatcagcaaa agtgatacgg gtattatttt tgccgctatt tctctgttct cgctattatt 3780 ccaaccgctg tttggtctgc tttctgacaa actcggaact tgtttattgc agcttataat 3840 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat 3900 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat cctctagagt 3960 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg 4020 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg 4080 gcagcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 4140 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 4200 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 4260 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 4320 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 4380 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 4440 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt 4500 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 4560 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 4620 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 4680 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 4740 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 4800 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4860 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4920 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4980 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 5040 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 5100 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 5160 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 5220 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 5280 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 5340 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 5400 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5460 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5520 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 5580 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 5640 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5700 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5760 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5820 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5880 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 5940 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 6000 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt 6060 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt 6120 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct 6180 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg 6240 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga 6300 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc 6360 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga 6420 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttccca 6480 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 6540 acgccagccc aagctaccat gataagtaag taatattaag gtacgtggag gttttacttg 6600 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 6660 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 6720 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 6780 tatcttatgg tactgtaact gagctaacat aa 6812 // ID AB062168; SV 1; linear; other DNA; STD; SYN; 4792 BP. XX AC AB062168; XX DT 28-NOV-2001 (Rel. 69, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Cloning vector pRTHSP70-GFP gene for green fluorescent protein GFP, DE promoter region and complete cds. XX KW . XX OS Cloning vector pRTHSP70-GFP OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-4792 RA Yamashita M., Ojima N.; RT ; RL Submitted (23-MAY-2001) to the INSDC. RL Michiaki Yamashita, National Research Institute of Fisheries Science; RL Fukuura-2, Yokohama, Kanagawa 236-8648, Japan (E-mail:mic@affrc.go.jp, RL Tel:81-45-788-7665, Fax:81-45-788-5001) XX RN [2] RA Yamashita M., Ojima N.; RT "Transgenic zebrafish as a biosensor for enviromental stress"; RL Unpublished. XX DR MD5; 495f5f09b59b4a9560d435a9efc499ce. XX FH Key Location/Qualifiers FH FT source 1..4792 FT /organism="Cloning vector pRTHSP70-GFP" FT /mol_type="other DNA" FT /db_xref="taxon:160967" FT regulatory 1..521 FT /note="SV40 late polyadenylation signal" FT /regulatory_class="other" FT regulatory 3913..4056 FT /gene="HSP70" FT /function="heat shock promoter" FT /note="rainbow trout HSP70" FT /regulatory_class="promoter" FT CDS 4074..4790 FT /codon_start=1 FT /transl_table=11 FT /gene="GFP" FT /product="green fluorescent protein GFP" FT /protein_id="BAB72232.1" FT /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FT FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG FT NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV FT NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FT FVTAAGITHGMDELYK" XX SQ Sequence 4792 BP; 1254 A; 1142 C; 1134 G; 1262 T; 0 other; ggccgctcta gcttgggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg 60 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat 120 gtgttaaact agctgcatat gcttgctgct tgagagtttt gcttactgag tatgatttat 180 gaaaatatta tacacaggag ctagtgattc taattgtttg tgtattttag attcacagtc 240 ccaaggctca tttcaggccc ctcagtcctc acagtctgtt catgatcata atcagccata 300 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga 360 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca 420 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 480 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatcgatcct gcattaatga 540 atcggccaac gcgcggggag aggcggtttg cgtattggct ggcgtaatag cgaagaggcc 600 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggac gcgccctgta 660 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 720 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 780 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 840 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 900 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 960 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 1020 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta 1080 acaaaatatt aacgtttaca atttcaggtg gcacttttcg gggaaatgtg cgcggaaccc 1140 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgcca ggtcttggac 1200 tggtgagaac ggcttgctcg gcagcttcga tgtgtgctgg agggagaata aaggtctaag 1260 atgtgcgata gagggaagtc gcattgaatt atgtgctgtg tagggatcgc tggtatcaaa 1320 tatgtgtgcc cacccctggc atgagacaat aaccctgata aatgcttcaa taatattgaa 1380 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat 1440 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc 1500 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga 1560 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg 1620 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc 1680 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag 1740 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc 1800 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg 1860 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg 1920 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac 1980 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac 2040 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg 2100 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg 2160 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg 2220 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac 2280 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg 2340 ataatctcat gccataactt cgtataatgt atgctatacg aagttatggc atgaccaaaa 2400 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 2460 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 2520 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 2580 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 2640 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 2700 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 2760 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 2820 cgacctacac cgaactgaga tacctacagc gtgagcattg agaaagcgcc acgcttcccg 2880 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 2940 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 3000 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 3060 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 3120 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 3180 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 3240 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcaga gcttgcaatt 3300 cgcgcgtttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 3360 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 3420 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 3480 ctacgaggcc ctttcactca ttaggcaccc caggctttac actttatagc ttccggctcg 3540 tataatgtgt ggaattgtga gcggataaca atttcacaca ggaaacagca tcgtgcaggt 3600 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 3660 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 3720 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 3780 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 3840 catgacctta tgggactttc ctacttggca gtacatctac gggtcgagat cccccgggct 3900 gcaggaattc gattggttgt tattcaagaa tattcaggaa aacatctctg ttggttgaga 3960 ttgacattac ttgcacctag gttttcatag aataatatca atttctaaaa tatttcatta 4020 ttttgtaggc aactaactcc aaacctacaa tctagtcggc ggccgccgcc accatgagca 4080 agggcgagga actgttcact ggcgtggtcc caattctcgt ggaactggat ggcgatgtga 4140 atgggcacaa attttctgtc agcggagagg gtgaaggtga tgccacatac ggaaagctca 4200 ccctgaaatt catctgcacc actggaaagc tccctgtgcc atggccaaca ctggtcacta 4260 ccttcaccta tggcgtgcag tgcttttcca gatacccaga ccatatgaag cagcatgact 4320 ttttcaagag cgccatgccc gagggctatg tgcaggagag aaccatcttt ttcaaagatg 4380 acgggaacta caagacccgc gctgaagtca agttcgaagg tgacaccctg gtgaatagaa 4440 tcgagttgaa gggcattgac tttaaggaag atggaaacat tctcggccac aagctggaat 4500 acaactataa ctcccacaat gtgtacatca tggccgacaa gcaaaagaat ggcatcaagg 4560 tcaacttcaa gatcagacac aacattgagg atggatccgt gcagctggcc gaccattatc 4620 aacagaacac tccaatcggc gacggccctg tgctcctccc agacaaccat tacctgtcca 4680 cccagtctgc cctgtctaaa gatcccaacg aaaagagaga ccacatggtc ctgctggagt 4740 ttgtgaccgc tgctgggatc acacatggca tggacgagct gtacaagtga gc 4792 // ID AB062417; SV 1; linear; other DNA; STD; SYN; 1047 BP. XX AC AB062417; XX DT 10-JAN-2002 (Rel. 70, Created) DT 01-JUN-2003 (Rel. 76, Last updated, Version 3) XX DE Synthetic construct Bos taurus gene for rhodopsin, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1047 RA Terakita A., Kojima D., Shichida Y.; RT ; RL Submitted (26-MAY-2001) to the INSDC. RL Yoshinori Shichida, Kyoto University, Department of Biophysics, Graduate RL School of Science; Sakyo-ku, Kitashirakawa, Oiwake-cho, Kyoto, Kyoto RL 606-8502, Japan (E-mail:shichida@photo2.biophys.kyoto-u.ac.jp, RL Tel:81-75-753-4213, Fax:81-75-753-4210) XX RN [2] RX DOI; 10.1074/jbc.M104960200. RX PUBMED; 11606568. RA Terakita A., Yamashita T., Nimbari N., Kojima D., Shichida Y.; RT "Functional interaction between bovine rhodopsin and G protein transducin"; RL J Biol Chem 277(1):40-46(2002). XX DR MD5; 3c566d365bf3df68abbbdc7e48c2b2af. XX FH Key Location/Qualifiers FH FT source 1..1047 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="synthetic Bos taurus rhodopsin" FT /db_xref="taxon:32630" FT CDS 1..1047 FT /codon_start=1 FT /transl_table=11 FT /product="rhodopsin" FT /note="synthetic bovine rhodopsin" FT /protein_id="BAB83621.1" FT /translation="MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMF FT LLIMLGFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSLHGYFV FT FGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRFGENHAIMGVAFTWVM FT ALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEETNNESFVIYMFVVHFIIPLIVIFFC FT YGQLVFTVKEAAAQQQESATTQKAEKEVTRMVIIMVIAFLICWLPYAGVAFYIFTHQGS FT DFGPIFMTIPAFFAKTSAVYNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKT FT ETSQVAPA" XX SQ Sequence 1047 BP; 204 A; 338 C; 267 G; 238 T; 0 other; atgaacggga ccgagggccc aaacttctac gtgcctttct ccaacaagac gggcgtcgta 60 cgcagcccct tcgaggcgcc gcagtactac ctggctgagc catggcagtt cagcatgctg 120 gccgcctaca tgttcctgct gatcatgctt ggcttcccca tcaacttcct cacgctgtac 180 gtcacagtcc agcacaagaa gctgaggacc cccctcaact acatcctgct caacctggcc 240 gtggcagatc tcttcatggt gttcgggggc ttcaccacca ccctgtatac ctctctgcac 300 gggtacttcg tgttcggtcc gacgggctgc aacctcgagg gcttctttgc caccttaggc 360 ggtgaaattg cactgtggtc cttggtggtg ctagccatcg agcggtacgt agtggtgtgc 420 aagcccatga gcaacttccg cttcggggag aaccacgcca tcatgggcgt cgcattcacc 480 tgggtcatgg ctctggcctg tgcggccccc cccctcgtcg gctggtctag atacatcccg 540 gaggggatgc agtgctcgtg cgggatcgat tactacacgc cccacgagga gaccaacaat 600 gagtcgttcg tcatctacat gttcgttgta cacttcatca tccccctgat tgtcatattc 660 ttctgctacg ggcagctggt cttcaccgtc aaggaggctg cagcccagca gcaggagtcg 720 gccaccactc agaaggccga gaaggaggtc acgcgtatgg tcatcatcat ggtcatcgct 780 ttcctcatat gctggctgcc ctacgcaggt gtggcgttct acatcttcac ccatcaggga 840 tccgactttg gccccatctt catgaccatc ccggctttct ttgccaagac gtctgccgtc 900 tataaccccg tcatctacat catgatgaac aagcagttcc ggaactgcat ggtcaccact 960 ctctgctgtg gcaagaaccc cctaggtgac gacgaggcct ccacgaccgt gtccaagaca 1020 gagaccagcc aagtggcccc tgcctaa 1047 // ID AB066225; SV 1; linear; other DNA; STD; SYN; 456 BP. XX AC AB066225; XX DT 22-JAN-2002 (Rel. 70, Created) DT 01-JUN-2003 (Rel. 76, Last updated, Version 3) XX DE Synthetic construct Streptomyces carzinostaticus ncsA gene for DE neocarzinostatin apoprotein, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-456 RA Tomioka Y., Hishinuma T., Mizugaki M., Nozaki S., Hirama M.; RT ; RL Submitted (16-JUL-2001) to the INSDC. RL Yoshihisa Tomioka, Tohoku University Hospital, Department of Pharmaceutical RL Sciences; 1-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan RL (E-mail:ytomioka@sm.rim.or.jp, Tel:81-22-717-7543, Fax:81-22-717-7545) XX RN [2] RA Tomioka Y., Nozaki S., Hishinuma T., Inoue M., Nagumo Y., Tsuruta L.R., RA Hayashi K., Matsumoto T., Kato Y., Ishiwata S., Itoh K., Suzuki T., RA Hirama M., Mizugaki M.; RT "Design, Production and Characterization of Recombinant Neocarzinostatin RT Apoprotein in Escherichia coli"; RL Unpublished. XX DR MD5; e390dcc42750f359161a6043a4913369. XX FH Key Location/Qualifiers FH FT source 1..456 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /clone="pN89-1" FT /note="original mRNA isolated from Streptomyces FT carzinostaticus" FT /db_xref="taxon:32630" FT CDS 7..>456 FT /codon_start=1 FT /transl_table=11 FT /gene="ncsA" FT /product="neocarzinostatin apoprotein" FT /protein_id="BAB83894.1" FT /translation="MGGRLASSVIVWRKWRWAAAAVLGLAVGFQTPAVAAAPTATVTPS FT SGLSDGTVVKVAGAGLQAGTAYDVGQCAWVDTGVLACNPADFSSVTADANGSASTSLTV FT RRSFEGFLFDGTRWGTVDCTTAACQVGLSDAAGNGPEGVAISFNGT" XX SQ Sequence 456 BP; 71 A; 121 C; 187 G; 77 T; 0 other; ggatctatgg gtggccgatt agcatcatcc gtaatcgtgt ggcgaaagtg gcggtgggca 60 gcggcggcgg tgctgggcct ggcggtgggc tttcagaccc cggcggtggc ggccgcgccg 120 accgcgacgg tgaccccgag cagcggcctg agcgatggca cggtggtgaa agtggcgggc 180 gcgggcctgc aggcgggcac cgcgtatgat gtgggccaat gcgcatgggt ggatacgggc 240 gtgctggcat gtaacccggc ggattttagc agcgtgaccg cggatgcgaa tggcagcgct 300 agcaccagcc tgacggtgcg ccgcagcttt gaaggctttc tgtttgatgg cacccgctgg 360 ggcacggtgg attgcacgac ggcggcatgc caggtgggcc tgagcgatgc ggcgggcaat 420 ggcccggaag gcgtggcgat cagctttaac ggtacc 456 // ID AB066226; SV 1; linear; other DNA; STD; SYN; 507 BP. XX AC AB066226; XX DT 22-JAN-2002 (Rel. 70, Created) DT 01-JUN-2003 (Rel. 76, Last updated, Version 3) XX DE Synthetic construct Streptomyces carzinostaticus gene for his-tagged DE neocarzinostatin apoprotein, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-507 RA Tomioka Y., Hishinuma T., Mizugaki M., Nozaki S., Hirama M.; RT ; RL Submitted (16-JUL-2001) to the INSDC. RL Yoshihisa Tomioka, Tohoku University Hospital, Department of Pharmaceutical RL Sciences; 1-1, Seiryo-machi, Aoba-ku, Sendai, Miyagi 980-8574, Japan RL (E-mail:ytomioka@sm.rim.or.jp, Tel:81-22-717-7543, Fax:81-22-717-7545) XX RN [2] RA Tomioka Y., Nozaki S., Hishinuma T., Inoue M., Nagumo Y., Tsuruta L.R., RA Hayashi K., Matsumoto T., Kato Y., Ishiwata S., Itoh K., Suzuki T., RA Hirama M., Mizugaki M.; RT "Design, Production and Characterization of Recombinant Neocarzinostatin RT Apoprotein in Escherichia coli"; RL Unpublished. XX DR MD5; da74818c13792dd13623e1f5da66895e. XX FH Key Location/Qualifiers FH FT source 1..507 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /clone="p1R49" FT /note="original mRNA isolated from Streptomyces FT carzinostaticus" FT /db_xref="taxon:32630" FT CDS 1..450 FT /codon_start=1 FT /transl_table=11 FT /product="his-tagged neocarzinostatin apoprotein" FT /protein_id="BAB83895.1" FT /translation="MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSAAPTATVTP FT SSGLSDGTVVKVAGAGLQAGTAYDVGQCAWVDTGVLACNPADFSSVTADANGSASTSLT FT VRRSFEGFLFDGTRWGTVDCTTAACQVGLSDAAGNGPEGVAISFN" XX SQ Sequence 507 BP; 102 A; 127 C; 187 G; 91 T; 0 other; atgcggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa 60 atgggtcggg atctgtacga cgatgacgat aaggatcgat ggggatccgc cgcgccgacc 120 gcgacggtga ccccgagcag cggcctgagc gatggcacgg tggtgaaagt ggcgggcgcg 180 ggcctgcagg cgggcaccgc gtatgatgtg ggccaatgcg catgggtgga tacgggcgtg 240 ctggcatgta acccggcgga ttttagcagc gtgaccgcgg atgcgaatgg cagcgctagc 300 accagcctga cggtgcgccg cagctttgaa ggctttctgt ttgatggcac ccgctggggc 360 acggtggatt gcacgacggc ggcatgccag gtgggcctga gcgatgcggc gggcaatggc 420 ccggaaggcg tggcgatcag ctttaactag ggtaccatgg aattcgaagc ttgatccggc 480 tgctaacaaa gcccgaaagg aagctga 507 // ID AB067816; SV 1; linear; other DNA; STD; SYN; 28 BP. XX AC AB067816; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-D1S80 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-28 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 75e34d54be87c86f96ca8fccb33e1161. XX FH Key Location/Qualifiers FH FT source 1..28 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..28 FT /note="forward primer for human STS sts-D1S80 at 1p36" FT /note="sts-D1S80 obtained from clone B326A10, Human BAC FT library RPCI-11" XX SQ Sequence 28 BP; 7 A; 12 C; 6 G; 3 T; 0 other; gaaactggcc tccaaacact gcccgccg 28 // ID AB067817; SV 1; linear; other DNA; STD; SYN; 29 BP. XX AC AB067817; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-D1S80 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-29 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; faed14a346d9dfbdc23f34e7a94ad9e3. XX FH Key Location/Qualifiers FH FT source 1..29 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..29 FT /note="reverse primer for human STS sts-D1S80 at 1p36" FT /note="sts-D1S80 obtained from clone B326A10, Human BAC FT library RPCI-11" XX SQ Sequence 29 BP; 3 A; 8 C; 9 G; 9 T; 0 other; gtcttgttgg agatgcacgt gccccttgc 29 // ID AB067818; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067818; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-U70321 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; ba5843e8c14d83665c0551c4307cb100. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="forward primer for human STS sts-U70321 at 1p36" FT /note="sts-U70321 obtained from clones B326A10, B361M21, FT Human BAC library RPCI-11" XX SQ Sequence 18 BP; 5 A; 8 C; 3 G; 2 T; 0 other; actgcagcca acccagtc 18 // ID AB067819; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067819; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-U70321 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 3ef22e49dd1c475bd0c47bf3fdc96355. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="reverse primer for human STS sts-U70321 at 1p36" FT /note="sts-U70321 obtained from clones B326A10, B361M21, FT Human BAC library RPCI-11" XX SQ Sequence 18 BP; 4 A; 10 C; 2 G; 2 T; 0 other; ccctcgctca cagaccac 18 // ID AB067820; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067820; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-cos254-T3 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; d80955548f1f79e07db46c863c93f003. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-cos254-T3 at 1p36" FT /note="sts-cos254-T3 obtained from clones B326A10, B361M21, FT Human BAC library RPCI-11" XX SQ Sequence 20 BP; 9 A; 5 C; 5 G; 1 T; 0 other; cccaaaggac tgaaaacagg 20 // ID AB067821; SV 1; linear; other DNA; STD; SYN; 24 BP. XX AC AB067821; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-cos254-T3 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-24 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; f3a72403e2c3494a2247857523232a46. XX FH Key Location/Qualifiers FH FT source 1..24 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..24 FT /note="reverse primer for human STS sts-cos254-T3 at 1p36" FT /note="sts-cos254-T3 obtained from clones B326A10, B361M21, FT Human BAC library RPCI-11" XX SQ Sequence 24 BP; 3 A; 4 C; 6 G; 11 T; 0 other; cttgtacctg ttgatggact tttg 24 // ID AB067822; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067822; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-H90455 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; fd07333b3bff60dd47c0d8860996b12c. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="forward primer for human STS sts-H90455 at 1p36" FT /note="sts-H90455 obtained from clones B326A10, B361M21, FT Human BAC library RPCI-11" XX SQ Sequence 18 BP; 4 A; 5 C; 6 G; 3 T; 0 other; tgtgcagcag cctgagac 18 // ID AB067823; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067823; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-H90455 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; e8cc3042988e8fe89e7300397f0e2245. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-H90455 at 1p36" FT /note="sts-H90455 obtained from clones B326A10, B361M21, FT Human BAC library RPCI-11" XX SQ Sequence 20 BP; 3 A; 4 C; 6 G; 7 T; 0 other; caatgtctgc actgtttggg 20 // ID AB067824; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067824; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-stSG25740 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 4b7b35ec9624b0ad907f0ca65022763f. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="forward primer for human STS sts-stSG25740 at 1p36" FT /note="sts-stSG25740 obtained from clones B326A10, B361M21, FT Human BAC library RPCI-11" XX SQ Sequence 19 BP; 5 A; 6 C; 5 G; 3 T; 0 other; caggacaaag ctgcgtctc 19 // ID AB067825; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067825; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-stSG25740 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 378f0e1ccfd39682243323f3bd1c18f3. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-stSG25740 at 1p36" FT /note="sts-stSG25740 obtained from clones B326A10, B361M21, FT Human BAC library RPCI-11" XX SQ Sequence 20 BP; 2 A; 7 C; 4 G; 7 T; 0 other; ggctcttcag cgtcatcttc 20 // ID AB067826; SV 1; linear; other DNA; STD; SYN; 23 BP. XX AC AB067826; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-stSG43321 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-23 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; ccbea766c9e6866951d60a6ebd14b53d. XX FH Key Location/Qualifiers FH FT source 1..23 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..23 FT /note="forward primer for human STS sts-stSG43321 at 1p36" FT /note="sts-stSG43321 obtained from clones B326A10, B361M21, FT Human BAC library RPCI-11" XX SQ Sequence 23 BP; 7 A; 8 C; 1 G; 7 T; 0 other; atatttacat cctcccacag cat 23 // ID AB067827; SV 1; linear; other DNA; STD; SYN; 24 BP. XX AC AB067827; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-stSG43321 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-24 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; d33d0f946024606610d202262b7a4809. XX FH Key Location/Qualifiers FH FT source 1..24 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..24 FT /note="reverse primer for human STS sts-stSG43321 at 1p36" FT /note="sts-stSG43321 obtained from clones B326A10, B361M21, FT Human BAC library RPCI-11" XX SQ Sequence 24 BP; 5 A; 6 C; 9 G; 4 T; 0 other; cacagccaag gctggactgg gttg 24 // ID AB067828; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067828; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-AA029578 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; e8eec49677ad4780b380fad1d5d35b7e. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="forward primer for human STS sts-AA029578 at 1p36" FT /note="sts-AA029578 obtained from clones B326A10, FT B361M21and B118K9, Human BAC library RPCI-11" XX SQ Sequence 18 BP; 5 A; 5 C; 6 G; 2 T; 0 other; agaggcggaa cccacttg 18 // ID AB067829; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067829; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-AA029578 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 08d02a5d0dd0db008341313f2daa3cb3. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="reverse primer for human STS sts-AA029578 at 1p36" FT /note="sts-AA029578 obtained from clones B326A10, B361M21, FT B118K9, Human BAC library RPCI-11" XX SQ Sequence 19 BP; 1 A; 4 C; 7 G; 7 T; 0 other; aggtgggctt tcctgtgtc 19 // ID AB067830; SV 1; linear; other DNA; STD; SYN; 16 BP. XX AC AB067830; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-stSG3242 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-16 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; bf308aef6d93493db473fb3ab1249f12. XX FH Key Location/Qualifiers FH FT source 1..16 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..16 FT /note="forward primer for human STS sts-stSG3242 at 1p36" FT /note="sts-stSG3242 obtained from clones B326A10, FT B361M21and B118K9, B118D12, Human BAC library RPCI-11" XX SQ Sequence 16 BP; 3 A; 3 C; 7 G; 3 T; 0 other; ggacatggga cttgcg 16 // ID AB067831; SV 1; linear; other DNA; STD; SYN; 16 BP. XX AC AB067831; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-stSG3242 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-16 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; f8ee464c3e832e63420f9020d6774509. XX FH Key Location/Qualifiers FH FT source 1..16 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..16 FT /note="reverse primer for human STS sts-stSG3242 at 1p36" FT /note="sts-stSG3242 obtained from clones B326A10, B361M21, FT B118K9, B118D12, Human BAC library RPCI-11" XX SQ Sequence 16 BP; 3 A; 4 C; 6 G; 3 T; 0 other; gcaacgaggg gctttc 16 // ID AB067832; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067832; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-SGC30638 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; b81102b8a162cd39fced9385f8c957d1. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="forward primer for human STS sts-SGC30638 at 1p36" FT /note="sts-SGC30638 obtained from clones B326A10, FT B361M21and B118K9, B118D12, Human BAC library RPCI-11" XX SQ Sequence 18 BP; 6 A; 6 C; 3 G; 3 T; 0 other; tgcaacccac ctgaatga 18 // ID AB067833; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067833; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-SGC30638 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 1a10c28f1514d0d629621920c43c151d. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="reverse primer for human STS sts-SGC30638 at 1p36" FT /note="sts-SGC30638 obtained from clones B326A10, B361M21, FT B118K9, B118D12, Human BAC library RPCI-11" XX SQ Sequence 19 BP; 6 A; 5 C; 5 G; 3 T; 0 other; tacgtgaagt gccagcaca 19 // ID AB067834; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067834; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-R361M21R at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; bef0054b87a6bae4e8d55ccc9d1a88bf. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-R361M21R at 1p36" FT /note="sts-R361M21R obtained from clones B326A10, FT B361M21and B118K9, B118D12, B105E22, Human BAC library FT RPCI-11" XX SQ Sequence 20 BP; 6 A; 5 C; 6 G; 3 T; 0 other; acgcagatgg atggactacc 20 // ID AB067835; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067835; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-R361M21R at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 8af742a43dfbc91395fa0fed248aeb29. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="reverse primer for human STS sts-R361M21R at 1p36" FT /note="sts-R361M21R obtained from clones B326A10, B361M21, FT B118K9, B118D12, B105E22, Human BAC library RPCI-11" XX SQ Sequence 18 BP; 2 A; 8 C; 3 G; 5 T; 0 other; accttgtctg ctccacgc 18 // ID AB067836; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067836; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-stSG9144 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 1098493bf376d7bf3796fca8e20b8f2a. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-stSG9144 at 1p36" FT /note="sts-stSG9144 obtained from clones B83K22, B118K9, FT B118D12, B105E22, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 7 A; 4 C; 5 G; 4 T; 0 other; gaggcaatga aattccctga 20 // ID AB067837; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067837; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-stSG9144 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; e6f95862d87f3c3eaa35fad2218df08b. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-stSG9144 at 1p36" FT /note="sts-stSG9144 obtained from clones B83K22, B118K9, FT B118D12, B105E22, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 4 A; 4 C; 6 G; 6 T; 0 other; caacggatcc tgagtggttt 20 // ID AB067838; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067838; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-stSG30502 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; b715f0169631cf51b81c48a3fb2eb864. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="forward primer for human STS sts-stSG30502 at 1p36" FT /note="sts-stSG30502 obtained from clones B83K22, B83I23, FT B105E22, Human BAC library RPCI-11" XX SQ Sequence 19 BP; 6 A; 7 C; 2 G; 4 T; 0 other; caaccattgc caaaccttg 19 // ID AB067839; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067839; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-stSG30502 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 59cadc0b3345341ee9ce211f25fd3086. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-stSG30502 at 1p36" FT /note="sts-stSG30502 obtained from clones B83K22, B83I23, FT B105E22, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 6 A; 6 C; 5 G; 3 T; 0 other; cggtcttcag aaaccagagc 20 // ID AB067840; SV 1; linear; other DNA; STD; SYN; 22 BP. XX AC AB067840; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-stSG29455 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-22 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; a78109b3ae100f1423acd8bc08d3f684. XX FH Key Location/Qualifiers FH FT source 1..22 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..22 FT /note="forward primer for human STS sts-stSG29455 at 1p36" FT /note="sts-stSG29455 obtained from clones B83K22, B83I23, FT B105E22, Human BAC library RPCI-11" XX SQ Sequence 22 BP; 8 A; 3 C; 6 G; 5 T; 0 other; aactgcataa aattgggact gg 22 // ID AB067841; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067841; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-stSG29455 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 46402d1296eaad9de30db6d583bae868. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-stSG29455 at 1p36" FT /note="sts-stSG29455 obtained from clones B83K22, B83I23, FT B105E22, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 5 A; 5 C; 6 G; 4 T; 0 other; ctggcaagct gtgacacagt 20 // ID AB067842; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067842; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-stGDB:636086-E002 DE at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 63736441f2bcc0755588ca93d9e1e5f6. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-stGDB:636086-E002 FT at 1p36" FT /note="sts-stGDB:636086-E002 obtained from clones B83K22, FT B83I23, B43E2, B105E22, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 0 A; 5 C; 4 G; 11 T; 0 other; gctgttgttt ttcccttcgt 20 // ID AB067843; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067843; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-stGDB:636086-E002 DE at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; b49cb682f7de06b8e8c6ba3f329e3da3. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="reverse primer for human STS sts-stGDB:636086-E002 FT at 1p36" FT /note="sts-stGDB:636086-E002 obtained from clones B83K22, FT B83I23, B43E2, B105E22, Human BAC library RPCI-11" XX SQ Sequence 19 BP; 8 A; 2 C; 9 G; 0 T; 0 other; caagcagaag ggggaagag 19 // ID AB067844; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067844; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-D1S2515 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 59f1cb47ce031639802dc439a79bb742. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-D1S2515 at 1p36" FT /note="sts-D1S2515 obtained from clones B83K22, B83I23, FT B43E2, B105E22, B128A8, B128H2, B181G12, Human BAC library FT RPCI-11" XX SQ Sequence 20 BP; 8 A; 5 C; 4 G; 3 T; 0 other; aaggacccag catatacatg 20 // ID AB067845; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067845; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-D1S2515 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 69093b71391b62231ecbc0204724a7a9. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-D1S2515 at 1p36" FT /note="sts-D1S2515 obtained from clones B83K22, B83I23, FT B43E2, B105E22, B128A8, B128H2, B181G12, Human BAC library FT RPCI-11" XX SQ Sequence 20 BP; 3 A; 4 C; 4 G; 9 T; 0 other; cgtaatgtct gcttttcgta 20 // ID AB067846; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067846; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-R47P3R at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 05c1a8553437c3865cd2975291cdd0e6. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="forward primer for human STS sts-R47P3R at 1p36" FT /note="sts-R47P3R obtained from clones B83K22, B47P3, FT B43E2, B123D13, B128A8, B128H2, B181G12, B226P2, Human BAC FT library RPCI-11" XX SQ Sequence 18 BP; 4 A; 4 C; 6 G; 4 T; 0 other; ggatgctgcc atgacatg 18 // ID AB067847; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067847; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-R47P3R at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; e8d52f5a73691a2572497bc4dca7444a. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="reverse primer for human STS sts-R47P3R at 1p36" FT /note="sts-R47P3R obtained from clones B83K22, B47P3, FT B43E2, B123D13, B128A8, B128H2, B181G12, B226P2, Human BAC FT library RPCI-11" XX SQ Sequence 18 BP; 2 A; 2 C; 9 G; 5 T; 0 other; ggctttgtgg caggtgag 18 // ID AB067848; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067848; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-D1S243 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 9192d26fb40d422f30072e1e8e3ecc1c. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="forward primer for human STS sts-D1S243 at 1p36" FT /note="sts-D1S243 obtained from clonesB83K22, B47P3, B43E2, FT B123D13, B290B2, B82D16,, B226P2, Human BAC library FT RPCI-11" XX SQ Sequence 18 BP; 5 A; 8 C; 3 G; 2 T; 0 other; cacacaggct cacatgcc 18 // ID AB067849; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067849; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-D1S243 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 8ecd3fbc2c352dc16d95d8c4b49ed325. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="reverse primer for human STS sts-D1S243 at 1p36" FT /note="sts-D1S243 obtained from clones B83K22, B47P3, FT B43E2, B123D13, B290B2and B82D16, B226P2, Human BAC library FT RPCI-11" XX SQ Sequence 18 BP; 3 A; 6 C; 5 G; 4 T; 0 other; gctccagcgt catggact 18 // ID AB067850; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067850; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-stSG1697 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; bcd8cb3bb45c1b87150f6c92bfd29048. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-stSG1697 at 1p36" FT /note="sts-stSG1697 obtained from clones B83K22, B47P3, FT B43E2, B123D13, B290B2, B82D16 , Human BAC library RPCI-11" XX SQ Sequence 20 BP; 5 A; 5 C; 5 G; 5 T; 0 other; ggaacacttt cctgagatgc 20 // ID AB067851; SV 1; linear; other DNA; STD; SYN; 24 BP. XX AC AB067851; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-stSG1697 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-24 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 134dc97a9a22a94387a99e91066b199d. XX FH Key Location/Qualifiers FH FT source 1..24 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..24 FT /note="reverse primer for human STS sts-stSG1697 at 1p36" FT /note="sts-stSG1697 obtained from clones B83K22, B47P3, FT B43E2, B123D13, B290B2and B82D16 , Human BAC library FT RPCI-11" XX SQ Sequence 24 BP; 8 A; 7 C; 4 G; 5 T; 0 other; cccatacacc agatttagag agtc 24 // ID AB067852; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067852; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-SGC33169 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 5ecffe51132db383d0f760fb21a2c6dd. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-SGC33169 at 1p36" FT /note="sts-SGC33169 obtained from clones B83K22, B47P3, FT B43E2, B123D13, B290B2, B82D16, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 6 A; 2 C; 7 G; 5 T; 0 other; tttgatggag aaatccgagg 20 // ID AB067853; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067853; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-SGC33169 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 4d9c9d48bd14a5ea21a7cf00df130b97. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="reverse primer for human STS sts-SGC33169 at 1p36" FT /note="sts-SGC33169 obtained from clones B83K22, B47P3, FT B43E2, B123D13, B290B2, B82D16, Human BAC library RPCI-11" XX SQ Sequence 18 BP; 6 A; 7 C; 4 G; 1 T; 0 other; cacagcggac atcacagc 18 // ID AB067854; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067854; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-R43E2R at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 819634cc5063093ad1f19e51fced0014. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="forward primer for human STS sts-R43E2R at 1p36" FT /note="sts-R43E2R obtained from clones B83K22, B47P3, FT B43E2, B290B2, B82D16, Human BAC library RPCI-11" XX SQ Sequence 19 BP; 2 A; 11 C; 1 G; 5 T; 0 other; tacactccct ccctgtccc 19 // ID AB067855; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067855; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-R43E2R at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 7d3b7086d9af926daa84a220b79fef9d. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="reverse primer for human STS sts-R43E2R at 1p36" FT /note="sts-R43E2R obtained from clones B83K22, B47P3, FT B43E2, B290B2, B82D16, Human BAC library RPCI-11" XX SQ Sequence 19 BP; 8 A; 5 C; 5 G; 1 T; 0 other; aaatgagcca ccgaaggac 19 // ID AB067856; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067856; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-R47P3F at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; d35c5b3f21aeaf504de16e4d3f1cd4d7. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-R47P3F at 1p36" FT /note="sts-R47P3F obtained from clones B83K22, B47P3, FT B290B2, B82D16, B142C13, B156O18, B156P17, Human BAC FT library RPCI-11" XX SQ Sequence 20 BP; 5 A; 5 C; 6 G; 4 T; 0 other; cacacccagg agtggagttt 20 // ID AB067857; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067857; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-R47P3F at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 9b6032312cb4710fcc5a1fabe869cc52. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="reverse primer for human STS sts-R47P3F at 1p36" FT /note="sts-R47P3F obtained from clones B83K22, B83I23, FT B290B2, B82D16, B142C13, B156O18, B156P17, Human BAC FT library RPCI-11" XX SQ Sequence 19 BP; 7 A; 6 C; 5 G; 1 T; 0 other; acacaggaca accagctgg 19 // ID AB067858; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067858; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-WI-16874 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 3cba5d2204d4113381c4a2f2cd6708d0. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="forward primer for human STS sts-WI-16874 at 1p36" FT /note="sts-WI-16874 obtained from clones B83K22, B290B2, FT B82D16, B142C13, B156O18, B156P17, Human BAC library FT RPCI-11" XX SQ Sequence 19 BP; 4 A; 4 C; 6 G; 5 T; 0 other; atggttcatc agcagctgg 19 // ID AB067859; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067859; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-WI-16874 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; b91cc5034a23f0fefd1c0603ae4ed415. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-WI-16874 at 1p36" FT /note="sts-WI-16874 obtained from clones B83K22, B290B2, FT B82D16, B142C13, B156O18, B156P17, Human BAC library FT RPCI-11" XX SQ Sequence 20 BP; 3 A; 8 C; 3 G; 6 T; 0 other; cctcacgctt ctcttcaagg 20 // ID AB067860; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067860; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-R367B21F at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 49002d7277df2ff6d8cfbccc73cf3a51. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-R367B21F at 1p36" FT /note="sts-R367B21F obtained from clones B367B21, B82D16, FT B142C13, B1D21, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 1 A; 6 C; 4 G; 9 T; 0 other; tgattgccct tgtctgtcct 20 // ID AB067861; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067861; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-R367B21F at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; c5112da9b84006bf090ddfdb16e2da53. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-R367B21F at 1p36" FT /note="sts-R367B21F obtained from clones B367B21, B82D16, FT B142C13, B1D21, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 5 A; 6 C; 4 G; 5 T; 0 other; ccaacttgag tcctgatgca 20 // ID AB067862; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067862; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-D1S1341 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 1fd82624b60222db406710c549acd12e. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-D1S1341 at 1p36" FT /note="sts-D1S1341 obtained from clones B345I6, B367B21, FT B346P1, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 2 A; 3 C; 8 G; 7 T; 0 other; ccttggggtc aggttaggtt 20 // ID AB067863; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067863; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-D1S1341 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 6a2363fbd284b232a5aeca496c5c585f. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-D1S1341 at 1p36" FT /note="sts-D1S1341 obtained from clones B345I6, B367B21, FT B346P1, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 4 A; 6 C; 4 G; 6 T; 0 other; attccttggt cacgtccaag 20 // ID AB067864; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067864; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-D1S3023 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 26f6dd29ee779542ff4998c208ac3225. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-D1S3023 at 1p36" FT /note="sts-D1S3023 obtained from clones B345I6, B367B21, FT B346P1, B367A21, B320J1, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 8 A; 2 C; 6 G; 4 T; 0 other; agttggagcg atgataaaca 20 // ID AB067865; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067865; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-D1S3023 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 2b3febbb88783fda763a292beadb7ce7. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="reverse primer for human STS sts-D1S3023 at 1p36" FT /note="sts-D1S3023 obtained from clones B345I6, B367B21, FT B346P1, B367A21, B320J1, Human BAC library RPCI-11" XX SQ Sequence 19 BP; 8 A; 4 C; 3 G; 4 T; 0 other; gtgttccaat aaacacgaa 19 // ID AB067866; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067866; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-NIB1364 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 184fd3cb83a5b8669f0d550f9656bff0. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-NIB1364 at 1p36" FT /note="sts-NIB1364 obtained from clones B345I6, B367B21, FT B346P1, B367A21, B320J1, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 6 A; 8 C; 2 G; 4 T; 0 other; aattaccctc tgccacaacg 20 // ID AB067867; SV 1; linear; other DNA; STD; SYN; 21 BP. XX AC AB067867; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-NIB1364 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-21 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 879803055bf6392101a363b5ca9e7121. XX FH Key Location/Qualifiers FH FT source 1..21 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..21 FT /note="reverse primer for human STS sts-NIB1364 at 1p36" FT /note="sts-NIB1364 obtained from clones B345I6, B367B21, FT B346P1, B367A21, B320J1, Human BAC library RPCI-11" XX SQ Sequence 21 BP; 3 A; 4 C; 7 G; 7 T; 0 other; tctgggcttc ttctaggaag g 21 // ID AB067868; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067868; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-R70N12R at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 5a884e229019c057a0d3e5cf042dd44f. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="forward primer for human STS sts-R70N12R at 1p36" FT /note="sts-R70N12R obtained from clones B345I6, B367B21, FT B346P1, B367A21, B70N12, B337F5, Human BAC library RPCI-11" XX SQ Sequence 19 BP; 5 A; 4 C; 6 G; 4 T; 0 other; ccgactggaa aacgtgttg 19 // ID AB067869; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067869; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-R70N12R at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 14993a31c2bee63cf7ae3f8c9e2aa249. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-R70N12R at 1p36" FT /note="sts-R70N12R obtained from clones B345I6, B367B21, FT B346P1, B367A21, B70N12, B337F5, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 3 A; 4 C; 6 G; 7 T; 0 other; tgggctcttc tggctaaatg 20 // ID AB067870; SV 1; linear; other DNA; STD; SYN; 22 BP. XX AC AB067870; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-R367B21R at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-22 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; b0e6316498fe13bbf75ee66d28435afb. XX FH Key Location/Qualifiers FH FT source 1..22 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..22 FT /note="forward primer for human STS sts-R367B21R at 1p36" FT /note="sts-R367B21R obtained from clones B54O7, B367B21, FT B367A21, B70N12, B337F5, B32B16, Human BAC library RPCI-11" XX SQ Sequence 22 BP; 2 A; 3 C; 6 G; 11 T; 0 other; gtgctaatgt ttttttcctg gg 22 // ID AB067871; SV 1; linear; other DNA; STD; SYN; 22 BP. XX AC AB067871; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-R367B21R at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-22 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 5bede054f58a66dec84b1ff58e2ea798. XX FH Key Location/Qualifiers FH FT source 1..22 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..22 FT /note="reverse primer for human STS sts-R367B21R at 1p36" FT /note="sts-R367B21R obtained from clones B54O7, B367B21, FT B367A21, B70N12, B337F5, B32B16, Human BAC library RPCI-11" XX SQ Sequence 22 BP; 7 A; 3 C; 6 G; 6 T; 0 other; ggcatgagaa gatttatcca tg 22 // ID AB067872; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067872; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-stSG15808 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; e4dfee05af4b56b734a7c5807caedf2a. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-stSG15808 at 1p36" FT /note="sts-stSG15808 obtained from clones B54O7, B175C20, FT Human BAC library RPCI-11" XX SQ Sequence 20 BP; 6 A; 4 C; 6 G; 4 T; 0 other; gcagattcat gaacacggtg 20 // ID AB067873; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067873; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-stSG15808 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; cb6002685426027b074b06535c750733. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="reverse primer for human STS sts-stSG15808 at 1p36" FT /note="sts-stSG15808 obtained from clones B54O7, B175C20, FT Human BAC library RPCI-11" XX SQ Sequence 18 BP; 7 A; 3 C; 7 G; 1 T; 0 other; aagcagcaag tgaacggg 18 // ID AB067874; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067874; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-stSG62963 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; e4dfee05af4b56b734a7c5807caedf2a. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-stSG62963 at 1p36" FT /note="sts-stSG62963 obtained from clones B54O7, Human BAC FT library RPCI-11" XX SQ Sequence 20 BP; 6 A; 4 C; 6 G; 4 T; 0 other; gcagattcat gaacacggtg 20 // ID AB067875; SV 1; linear; other DNA; STD; SYN; 18 BP. XX AC AB067875; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-stSG62963 at DE 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-18 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; cb6002685426027b074b06535c750733. XX FH Key Location/Qualifiers FH FT source 1..18 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..18 FT /note="reverse primer for human STS sts-stSG62963 at 1p36" FT /note="sts-stSG62963 obtained from clones B54O7, Human BAC FT library RPCI-11" XX SQ Sequence 18 BP; 7 A; 3 C; 7 G; 1 T; 0 other; aagcagcaag tgaacggg 18 // ID AB067876; SV 1; linear; other DNA; STD; SYN; 19 BP. XX AC AB067876; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-WIAF-768 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-19 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; a9b06f9e69a16221b52c3cc5c3bb8e5a. XX FH Key Location/Qualifiers FH FT source 1..19 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..19 FT /note="forward primer for human STS sts-WIAF-768 at 1p36" FT /note="sts-WIAF-768 obtained from clones B54O7, B38G1, FT B38G3, Human BAC library RPCI-11" XX SQ Sequence 19 BP; 1 A; 8 C; 1 G; 9 T; 0 other; tttttttcct gcctcccac 19 // ID AB067877; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067877; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-WIAF-768 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 7ce7dd5175c18ec6df24a4ce3d13ac20. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-WIAF-768 at 1p36" FT /note="sts-WIAF-768 obtained from clones B54O7, B38G1, FT B38G3, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 5 A; 7 C; 5 G; 3 T; 0 other; gagaccagtc ccagttccag 20 // ID AB067878; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067878; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, forward primer for human STS sts-WI-15347 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 9012da721746a9030b53486a1f6e0244. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="forward primer for human STS sts-WI-15347 at 1p36" FT /note="sts-WI-15347 obtained from clones B54O7, B38G1, FT B14F12, Human BAC library RPCI-11" XX SQ Sequence 20 BP; 7 A; 2 C; 8 G; 3 T; 0 other; caaagacttg ggggagtgaa 20 // ID AB067879; SV 1; linear; other DNA; STD; SYN; 20 BP. XX AC AB067879; XX DT 08-AUG-2001 (Rel. 68, Created) DT 21-MAY-2003 (Rel. 75, Last updated, Version 2) XX DE Synthetic construct DNA, reverse primer for human STS sts-WI-15347 at 1p36. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-20 RA Horii A.; RT ; RL Submitted (04-AUG-2001) to the INSDC. RL Akira Horii, Tohoku University School of Medicine, Molecular Pathology; 2-1 RL Seiryomachi, Aoba-ku, Sendai, Miyagi 980-8575, Japan RL (E-mail:horii@mail.cc.tohoku.ac.jp, Tel:81-22-717-8042, Fax:81-22-717-8047) XX RN [2] RX DOI; 10.1006/geno.2001.6511. RX PUBMED; 11374902. RA Chen Y.Z., Hayashi Y., Wu J.G., Takaoka E., Maekawa K., Watanabe N., RA Inazawa J., Hosoda F., Arai Y., Mizushima H., Morohashi A., Ohira M., RA Nakagawara A., Liu S., Hoshi M., Horii A., Soeda E.; RT "A BAC-based STS-content map spanning a 35-Mb region of human chromosome RT 1p35-p36"; RL Genomics 74(1):55-70(2001). XX DR MD5; 7ce7dd5175c18ec6df24a4ce3d13ac20. XX FH Key Location/Qualifiers FH FT source 1..20 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature 1..20 FT /note="reverse primer for human STS sts-WI-15347 at 1p36" FT /note="sts-WI-15347 obtained from clones B54O7, B38G1, FT