ID SCPV02062715; SV 1; linear; genomic DNA; STD; VRT; 812 BP. XX AC SCPV02062715; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22364.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-812 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-812 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; ddb33b2f2170f69b1f8b952fb1141c31. DR ENA-CON; ML191181. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..812 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22364.2" XX SQ Sequence 812 BP; 193 A; 174 C; 219 G; 226 T; 0 other; agtgtctatg ccttgcttgc atagaacccg gaggaaagca caccctgagt tagttgtgta 60 taaaggccct cacctgggcc cggtctagat gagactggtg tggcagggcc ctggggtcct 120 tgtagttaaa ggctaaccca ctgagtgggg cctctagact tgagcttttc ggtggcttta 180 tttgtctttg ggccgatgtg taagggattt gggtttaggg tttttgggct cccagagagc 240 taccatatca tttcttcatt ttctgtctgg accagagagg cagagatgga gagaaaaaaa 300 aaatatggtt gttttaaagt caaatctgcc tgtctgcctc ctgagagctg ggattaaagg 360 catgtgccac caaacccgag ccaggcagtg tctttattaa aaaatatttg aaaacctagt 420 ggaggtggtg gttcttgctt ttaatccagc ccttgggagg tagaggtagg cagatctccg 480 agttcaaggc cagcctggcc tacagagtca cttccagagc tacacagaga aactttggag 540 gagaaaaaaa aaattaaaag aagcggctca gactagctgc tttgttaact ttttttttgc 600 taatgctggt ttctttctat tccctctgct ggtagggacc cgagtgtaca gtcctccaga 660 gtggattcgc taccatcgct accacggcag gtcggcagct gtctggtccc ttgggatcct 720 gctctatgac atggtctgcg gagatattcc gtttgagcac gatgaagaga tcatcaaggg 780 ccaagtgttc ttcaggcaaa ctgtctcttc ag 812 // ID SCPV02062716; SV 1; linear; genomic DNA; STD; VRT; 822 BP. XX AC SCPV02062716; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22365.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-822 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-822 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4d087fc9e4975a15ddad2b3c1207be47. DR ENA-CON; ML191182. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..822 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22365.1" XX SQ Sequence 822 BP; 246 A; 163 C; 223 G; 190 T; 0 other; ccgtttctta tatgggggtt gcgaggcccg gcggtggcgg ctggcggcgg cggctgtgag 60 gggttcccgg aagatggtgc tgattaagga attccgtgtg gttttgccgt gttctgttca 120 agagtatcag gttggacagc tttactctgt tgcagaagcg agtaagaatg aaactggtgg 180 tggagaagga atcgaagtcc tgaagaatga accttatgaa aacgatggcg agaagggaca 240 gtacacacac aaaatctacc acctgaagag caaagttcct gcatttgtga ggatgattgc 300 tcccgagggc tccctggtgt ttcatgagaa agcctggaat gcctacccct actgcagaac 360 aattgtaacg aatgaataca tgaaagatga cttcttcatc aaaattgaaa catggcataa 420 acctgacttg ggaacattag aaaatgttca cggtttagat cccaacactt ggaaaactgt 480 tgaaattgtc cacatagaca ttgcagatcg aagtcaagtt gaaccagcag actacaaagc 540 tgatgaagac cctgcattat tccattcagt caagaccaag agaggacccc tgggacctaa 600 ctggaagaag gagctggcaa acacccctga ctgtcctagg atgtgtgcct ataagctggt 660 gaccatcaag ttcaagtggt gggggctgca gagcaaagta gagaacttta tccagaagca 720 agaaaaacgg atatttacga acttacatcg ccagctcttt tgttggattg acaagtggat 780 tgacctgaca atggaagaca ttaggcgaat ggaggatgag ac 822 // ID SCPV02062717; SV 1; linear; genomic DNA; STD; VRT; 822 BP. XX AC SCPV02062717; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22365.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-822 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-822 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4d087fc9e4975a15ddad2b3c1207be47. DR ENA-CON; ML191182. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..822 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22365.2" XX SQ Sequence 822 BP; 246 A; 163 C; 223 G; 190 T; 0 other; ccgtttctta tatgggggtt gcgaggcccg gcggtggcgg ctggcggcgg cggctgtgag 60 gggttcccgg aagatggtgc tgattaagga attccgtgtg gttttgccgt gttctgttca 120 agagtatcag gttggacagc tttactctgt tgcagaagcg agtaagaatg aaactggtgg 180 tggagaagga atcgaagtcc tgaagaatga accttatgaa aacgatggcg agaagggaca 240 gtacacacac aaaatctacc acctgaagag caaagttcct gcatttgtga ggatgattgc 300 tcccgagggc tccctggtgt ttcatgagaa agcctggaat gcctacccct actgcagaac 360 aattgtaacg aatgaataca tgaaagatga cttcttcatc aaaattgaaa catggcataa 420 acctgacttg ggaacattag aaaatgttca cggtttagat cccaacactt ggaaaactgt 480 tgaaattgtc cacatagaca ttgcagatcg aagtcaagtt gaaccagcag actacaaagc 540 tgatgaagac cctgcattat tccattcagt caagaccaag agaggacccc tgggacctaa 600 ctggaagaag gagctggcaa acacccctga ctgtcctagg atgtgtgcct ataagctggt 660 gaccatcaag ttcaagtggt gggggctgca gagcaaagta gagaacttta tccagaagca 720 agaaaaacgg atatttacga acttacatcg ccagctcttt tgttggattg acaagtggat 780 tgacctgaca atggaagaca ttaggcgaat ggaggatgag ac 822 // ID SCPV02062718; SV 1; linear; genomic DNA; STD; VRT; 751 BP. XX AC SCPV02062718; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22366.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-751 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-751 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 0464be28d8c5d5d1f5ba4f17f9943bc6. DR ENA-CON; ML191183. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..751 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22366.1" XX SQ Sequence 751 BP; 199 A; 187 C; 243 G; 122 T; 0 other; gagcgcgcgc gagcgcagca gtgggaggcg gcgactggcc ggcagaagcc tcagcaccat 60 gtggcacgag gctcggaagc atgagcggaa acttcgaggc atgatggtgg actacaagaa 120 gagggcggag cggcgtcggg agtactatga gaagattaag aaagaccccg ctcagttcct 180 gcaggtgcat ggccgagcct gcaaggttca cctggattct gcagttgccc tggctgctga 240 gagccctgtg aacatgatgc cctggcaggg ggacaccaac aacatgattg accgctttga 300 tgttcgtgca catctagacc acatccctga ctacaccccc ccactgctca ccaccatctc 360 cccggaacag gagtcagacg agcggaaatg taactacgag cggtacagag gcctggtgca 420 gaacgacttc gccggcatct ctgaggaaca gtgcttgtat cagatctaca ttgatgagct 480 gtacggaggc ctgcagagac ccagcgagga tgagaagaag aagctggcag agaagaaggc 540 ttccatcggc tacacctatg aagatagtac tgtggccgag gtagagaagg tggctgagaa 600 gccagaggag gaagagtcac cagcagagga ggagagcaac tcggatgaag atgaggtcat 660 ccccgacatc gacgtggagg tggatgtgga tgagctgaac caggagcagg tggctgatct 720 caataagcag gccacaacct acggcatggc t 751 // ID SCPV02062719; SV 1; linear; genomic DNA; STD; VRT; 751 BP. XX AC SCPV02062719; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22366.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-751 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-751 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 0464be28d8c5d5d1f5ba4f17f9943bc6. DR ENA-CON; ML191183. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..751 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22366.2" XX SQ Sequence 751 BP; 199 A; 187 C; 243 G; 122 T; 0 other; gagcgcgcgc gagcgcagca gtgggaggcg gcgactggcc ggcagaagcc tcagcaccat 60 gtggcacgag gctcggaagc atgagcggaa acttcgaggc atgatggtgg actacaagaa 120 gagggcggag cggcgtcggg agtactatga gaagattaag aaagaccccg ctcagttcct 180 gcaggtgcat ggccgagcct gcaaggttca cctggattct gcagttgccc tggctgctga 240 gagccctgtg aacatgatgc cctggcaggg ggacaccaac aacatgattg accgctttga 300 tgttcgtgca catctagacc acatccctga ctacaccccc ccactgctca ccaccatctc 360 cccggaacag gagtcagacg agcggaaatg taactacgag cggtacagag gcctggtgca 420 gaacgacttc gccggcatct ctgaggaaca gtgcttgtat cagatctaca ttgatgagct 480 gtacggaggc ctgcagagac ccagcgagga tgagaagaag aagctggcag agaagaaggc 540 ttccatcggc tacacctatg aagatagtac tgtggccgag gtagagaagg tggctgagaa 600 gccagaggag gaagagtcac cagcagagga ggagagcaac tcggatgaag atgaggtcat 660 ccccgacatc gacgtggagg tggatgtgga tgagctgaac caggagcagg tggctgatct 720 caataagcag gccacaacct acggcatggc t 751 // ID SCPV02062720; SV 1; linear; genomic DNA; STD; VRT; 630 BP. XX AC SCPV02062720; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22367.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-630 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-630 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 57bab29c4d1d07afb0ef00fb757ebb73. DR ENA-CON; ML191184. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..630 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22367.1" XX SQ Sequence 630 BP; 254 A; 139 C; 133 G; 104 T; 0 other; ccaggatccc agaagcttgg tcacaccagg atctcagggt cccagcttga ctcccacgaa 60 ctctgacaca cccaggatcc cagaatcaca gaatcccaga gaaagctgga ctctgaggaa 120 ttctgaccaa ccaagatcac aggaaagaca gatacagcca gggcaggtaa cactggagat 180 agccagatgg caggagggag gcataagaac ataagcaaca gaaaccaagg ttacttggca 240 tcatcagaac acaattctcc caccatagca agtccgggat acaccattac actggaaaag 300 caagattcag atctaaaatc acttttcata atgatgataa aggtctttaa gaagagcgta 360 aataactccc atataagaaa caggagaaca caggtaaaca gctagaagcc cttaaagagg 420 aaacaacaac aacaaaaatc ccttaaagaa ttacaggaaa acacaaacag gcaaaggaaa 480 tgaacaaaac catccaagat ctcaaagtgg taataaaaac aataaagaaa tcacaaaggg 540 agacaaccct ggagttagaa aatctaggaa aggggctgga gagatggctt agcagttaag 600 agcactgact gctcttctaa aggtcttaag 630 // ID SCPV02062721; SV 1; linear; genomic DNA; STD; VRT; 630 BP. XX AC SCPV02062721; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22367.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-630 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-630 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 57bab29c4d1d07afb0ef00fb757ebb73. DR ENA-CON; ML191184. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..630 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22367.2" XX SQ Sequence 630 BP; 254 A; 139 C; 133 G; 104 T; 0 other; ccaggatccc agaagcttgg tcacaccagg atctcagggt cccagcttga ctcccacgaa 60 ctctgacaca cccaggatcc cagaatcaca gaatcccaga gaaagctgga ctctgaggaa 120 ttctgaccaa ccaagatcac aggaaagaca gatacagcca gggcaggtaa cactggagat 180 agccagatgg caggagggag gcataagaac ataagcaaca gaaaccaagg ttacttggca 240 tcatcagaac acaattctcc caccatagca agtccgggat acaccattac actggaaaag 300 caagattcag atctaaaatc acttttcata atgatgataa aggtctttaa gaagagcgta 360 aataactccc atataagaaa caggagaaca caggtaaaca gctagaagcc cttaaagagg 420 aaacaacaac aacaaaaatc ccttaaagaa ttacaggaaa acacaaacag gcaaaggaaa 480 tgaacaaaac catccaagat ctcaaagtgg taataaaaac aataaagaaa tcacaaaggg 540 agacaaccct ggagttagaa aatctaggaa aggggctgga gagatggctt agcagttaag 600 agcactgact gctcttctaa aggtcttaag 630 // ID SCPV02062722; SV 1; linear; genomic DNA; STD; VRT; 708 BP. XX AC SCPV02062722; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22368.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-708 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-708 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 3cc4ea6d19c4c80657eedd53e2ff2371. DR ENA-CON; ML191185. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..708 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22368.1" XX SQ Sequence 708 BP; 166 A; 184 C; 227 G; 131 T; 0 other; gccgagcgcg gaacggtcat ggacggtacg ggtcaatccg ggcctcgggt cgggaaagcg 60 aggcgagggg cagcgctcgt cacgcgtgag gaaacgccta tcctccgccc ttccgacctg 120 gacttcactg cactgactgg aggggaagag aacgtggtcg gccaccgact ggcaacacgc 180 ccggcacgcc tctttcggcg gacgggcagg cgataccgga agtgggtggg gtctctttat 240 atgcttccgg cgactagcgg tgtctgagct gcaggtgagc ggccggttcc cgtccgcgtc 300 ccgccgacgg gcgactggac gccctcgcgg ccgtccggtg cagccttgcg agagctcaaa 360 catgaactat atgcccggca ccgccagcct catcgaggac atcgacaaaa agcacttggt 420 cctacttcga gatggaagaa cacttatagg ttttttaaga agcattgatc aatttgctaa 480 cttagtgctc catcagactg tggagcgaat tcatgtgggc aaaaagtacg gtgatattcc 540 tcgagggatt ttcgtggtca gaggagagaa tgtggtccta ctaggagaaa tagacctgga 600 gaaggagagt gacacgcccc ttcagcaagt gtctattgaa gagatcctag aggaacagag 660 ggtgcaacag cagaccagac tggaggcaga gaagctgaag gtccagac 708 // ID SCPV02062723; SV 1; linear; genomic DNA; STD; VRT; 708 BP. XX AC SCPV02062723; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22368.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-708 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-708 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 3cc4ea6d19c4c80657eedd53e2ff2371. DR ENA-CON; ML191185. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..708 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22368.2" XX SQ Sequence 708 BP; 166 A; 184 C; 227 G; 131 T; 0 other; gccgagcgcg gaacggtcat ggacggtacg ggtcaatccg ggcctcgggt cgggaaagcg 60 aggcgagggg cagcgctcgt cacgcgtgag gaaacgccta tcctccgccc ttccgacctg 120 gacttcactg cactgactgg aggggaagag aacgtggtcg gccaccgact ggcaacacgc 180 ccggcacgcc tctttcggcg gacgggcagg cgataccgga agtgggtggg gtctctttat 240 atgcttccgg cgactagcgg tgtctgagct gcaggtgagc ggccggttcc cgtccgcgtc 300 ccgccgacgg gcgactggac gccctcgcgg ccgtccggtg cagccttgcg agagctcaaa 360 catgaactat atgcccggca ccgccagcct catcgaggac atcgacaaaa agcacttggt 420 cctacttcga gatggaagaa cacttatagg ttttttaaga agcattgatc aatttgctaa 480 cttagtgctc catcagactg tggagcgaat tcatgtgggc aaaaagtacg gtgatattcc 540 tcgagggatt ttcgtggtca gaggagagaa tgtggtccta ctaggagaaa tagacctgga 600 gaaggagagt gacacgcccc ttcagcaagt gtctattgaa gagatcctag aggaacagag 660 ggtgcaacag cagaccagac tggaggcaga gaagctgaag gtccagac 708 // ID SCPV02062724; SV 1; linear; genomic DNA; STD; VRT; 803 BP. XX AC SCPV02062724; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22369.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-803 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-803 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 24f39cf76238be0aaa5d2617fa6f4e1a. DR ENA-CON; ML191186. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..803 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22369.1" XX SQ Sequence 803 BP; 143 A; 254 C; 261 G; 145 T; 0 other; ggggggggca ggtggggggg aggcactgca ggaaggcagc ggggaggggg ctggctcctt 60 gctccccgct gacgaggtgg gttccaggag cccctatcag cccagccagc tggcggctcc 120 cgggcctgcc tgccctagat aaggccgagc gctccctgcc taagcctgat tagtccgtgc 180 cgggaaagga gccgcattcc gggggggctt tgttgggagc attacaggga gcggcgcctg 240 ggcaggggct gatagcggca ctgggctgcc cagcctgctc tgtgccccat ggggatgggc 300 cctgggggag ctggaggtgg agcctgcgcg ggctgatgcc gaagagcctc tgtccagctc 360 tgctgggcca cgggtcttct gggtcgcagc ggtttgtcct acagccccca aacacccccg 420 ggggggaggg ccaagcccag tgatgagctg ccaaattttt aacaaccggt tccctccccc 480 ccccccagag ttctttgctc accctctggc cctttaacaa cgggttctcc atggaggtct 540 aattttagca actggttctt gagaacctgt gggagcctgc tccagctcac cactggccaa 600 gccctatttc atagcctgat aagctggccc taggtggaga aactgaggca tggggtggtg 660 tgacagatct cccagggagt cagcagcaga gtcacgatag aacccagcag tcctggctcc 720 cagcccccaa ctctaaccca ctagacccca ctcccctgcc agagccagga tagaacgcag 780 gaatcctggc tcccagtccc ccg 803 // ID SCPV02062725; SV 1; linear; genomic DNA; STD; VRT; 803 BP. XX AC SCPV02062725; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22369.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-803 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-803 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 24f39cf76238be0aaa5d2617fa6f4e1a. DR ENA-CON; ML191186. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..803 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22369.2" XX SQ Sequence 803 BP; 143 A; 254 C; 261 G; 145 T; 0 other; ggggggggca ggtggggggg aggcactgca ggaaggcagc ggggaggggg ctggctcctt 60 gctccccgct gacgaggtgg gttccaggag cccctatcag cccagccagc tggcggctcc 120 cgggcctgcc tgccctagat aaggccgagc gctccctgcc taagcctgat tagtccgtgc 180 cgggaaagga gccgcattcc gggggggctt tgttgggagc attacaggga gcggcgcctg 240 ggcaggggct gatagcggca ctgggctgcc cagcctgctc tgtgccccat ggggatgggc 300 cctgggggag ctggaggtgg agcctgcgcg ggctgatgcc gaagagcctc tgtccagctc 360 tgctgggcca cgggtcttct gggtcgcagc ggtttgtcct acagccccca aacacccccg 420 ggggggaggg ccaagcccag tgatgagctg ccaaattttt aacaaccggt tccctccccc 480 ccccccagag ttctttgctc accctctggc cctttaacaa cgggttctcc atggaggtct 540 aattttagca actggttctt gagaacctgt gggagcctgc tccagctcac cactggccaa 600 gccctatttc atagcctgat aagctggccc taggtggaga aactgaggca tggggtggtg 660 tgacagatct cccagggagt cagcagcaga gtcacgatag aacccagcag tcctggctcc 720 cagcccccaa ctctaaccca ctagacccca ctcccctgcc agagccagga tagaacgcag 780 gaatcctggc tcccagtccc ccg 803 // ID SCPV02062726; SV 1; linear; genomic DNA; STD; VRT; 733 BP. XX AC SCPV02062726; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22370.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-733 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-733 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 8f8e5ab8edd5450e5075102abb9068ac. DR ENA-CON; ML191187. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..733 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22370.1" XX SQ Sequence 733 BP; 248 A; 175 C; 131 G; 179 T; 0 other; cttaaaaatg cattaaattt tttccctaac aactccctgt gataaacaag gctgagtccc 60 cactctgtca ctttgtgtgc agaaagaggg gccccgcaag gattctaaaa attaatactt 120 gcaactccgg gcttgtattc aactatgaag gatacagctt ctctgacctt ggcttggtaa 180 acgctgccac ctcccaaatg caaagaaacc ccatttgaac ccaggaggag cacttgggaa 240 ttcctccctg tgggttaccc tcaagccctt tcaccctgct ctggggaaga gctgagaaag 300 aaaacaaagg agattagctg tggctatcag ctaatcaaac aacatgcaca aacctcttag 360 gtcaccaaaa atccaatcag cccctaaaaa aaagtaattt ttattaaaac ataaagaaag 420 aaaatacatc tggaacgtag gcatttgcta gattttaaaa gagcaattcc aaaaattaag 480 cactcaaact agcttcttgg gggtttagct taaacgttac aagcaaacaa aagcatcagg 540 ggttagcaca gaggagagcc acaaaccaaa ataagaaata aacctgatag tgtctaacta 600 aacattccct atccaaatta tttcttctcg gtatggaaaa tactttttca tacctggttc 660 aaatcttaca cagcattgct gctccgtgtt cctgcagccc agagaacaga taatgggaaa 720 gttccccccc ccc 733 // ID SCPV02062727; SV 1; linear; genomic DNA; STD; VRT; 733 BP. XX AC SCPV02062727; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22370.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-733 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-733 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 8f8e5ab8edd5450e5075102abb9068ac. DR ENA-CON; ML191187. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..733 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22370.2" XX SQ Sequence 733 BP; 248 A; 175 C; 131 G; 179 T; 0 other; cttaaaaatg cattaaattt tttccctaac aactccctgt gataaacaag gctgagtccc 60 cactctgtca ctttgtgtgc agaaagaggg gccccgcaag gattctaaaa attaatactt 120 gcaactccgg gcttgtattc aactatgaag gatacagctt ctctgacctt ggcttggtaa 180 acgctgccac ctcccaaatg caaagaaacc ccatttgaac ccaggaggag cacttgggaa 240 ttcctccctg tgggttaccc tcaagccctt tcaccctgct ctggggaaga gctgagaaag 300 aaaacaaagg agattagctg tggctatcag ctaatcaaac aacatgcaca aacctcttag 360 gtcaccaaaa atccaatcag cccctaaaaa aaagtaattt ttattaaaac ataaagaaag 420 aaaatacatc tggaacgtag gcatttgcta gattttaaaa gagcaattcc aaaaattaag 480 cactcaaact agcttcttgg gggtttagct taaacgttac aagcaaacaa aagcatcagg 540 ggttagcaca gaggagagcc acaaaccaaa ataagaaata aacctgatag tgtctaacta 600 aacattccct atccaaatta tttcttctcg gtatggaaaa tactttttca tacctggttc 660 aaatcttaca cagcattgct gctccgtgtt cctgcagccc agagaacaga taatgggaaa 720 gttccccccc ccc 733 // ID SCPV02062728; SV 1; linear; genomic DNA; STD; VRT; 857 BP. XX AC SCPV02062728; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22371.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-857 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-857 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; fd16ef0059ce4fddf19645f66b12f6a5. DR ENA-CON; ML191188. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..857 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22371.1" XX SQ Sequence 857 BP; 169 A; 271 C; 199 G; 218 T; 0 other; ttaaattgca gcaagggcgg tttaggttgg acattagaaa aacttcccac ctgttcgggt 60 ggttcagcac tggaataaat tgcccgggga ggttgtggaa tctccaccac tggggatttt 120 taagagctgg ttggacaaac acctgtcagg gatgggccag atcagtggtt cccgaatcgt 180 accaacctgc gaaccccctt tcactaaaat gtcgagtctc ttgaaccccc tcctaaaaat 240 gaacatttcc aggcgttttc tcccataacc gatggaagcc gcgatcttgg aaataatttg 300 gtaccttatg acacatcctt tctgttgaca ttattattta tcattccatt attaacgtcg 360 ttacgtcatg aaaacggcaa cactcttccc agagtcactc ctgtagctcg agtcacttcg 420 attcagcctc ctccgtgtct tatcagggag tatcggacgg gaactaaccc ctagtcctgc 480 cgtgagtggg ggggcctggg ctagacgacc tctcgaggtc ccttccagtc ctgtgcttct 540 aggattcccc gactcccgcc cccctccctg ctgcggtctc tggcccccac ccagcttcag 600 ctgtttccag ccccacccag ggcttccctc agggttccca ccccagcccc atggcaacca 660 ccacatgaaa caaacacact gcagccagcg ggcacgttga gctgtgacgc ccttcactgc 720 tgtcccggac gcctgggtcc tcccctcccc ccgctgcccg ggctacgtct ctctgccgtc 780 ctggatgcct gggtcctgtt ttcataccct gaagcttttc aggtggcaag cagctgtgct 840 cctggtcttg ttgcttg 857 // ID SCPV02062729; SV 1; linear; genomic DNA; STD; VRT; 857 BP. XX AC SCPV02062729; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22371.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-857 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-857 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; fd16ef0059ce4fddf19645f66b12f6a5. DR ENA-CON; ML191188. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..857 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22371.2" XX SQ Sequence 857 BP; 169 A; 271 C; 199 G; 218 T; 0 other; ttaaattgca gcaagggcgg tttaggttgg acattagaaa aacttcccac ctgttcgggt 60 ggttcagcac tggaataaat tgcccgggga ggttgtggaa tctccaccac tggggatttt 120 taagagctgg ttggacaaac acctgtcagg gatgggccag atcagtggtt cccgaatcgt 180 accaacctgc gaaccccctt tcactaaaat gtcgagtctc ttgaaccccc tcctaaaaat 240 gaacatttcc aggcgttttc tcccataacc gatggaagcc gcgatcttgg aaataatttg 300 gtaccttatg acacatcctt tctgttgaca ttattattta tcattccatt attaacgtcg 360 ttacgtcatg aaaacggcaa cactcttccc agagtcactc ctgtagctcg agtcacttcg 420 attcagcctc ctccgtgtct tatcagggag tatcggacgg gaactaaccc ctagtcctgc 480 cgtgagtggg ggggcctggg ctagacgacc tctcgaggtc ccttccagtc ctgtgcttct 540 aggattcccc gactcccgcc cccctccctg ctgcggtctc tggcccccac ccagcttcag 600 ctgtttccag ccccacccag ggcttccctc agggttccca ccccagcccc atggcaacca 660 ccacatgaaa caaacacact gcagccagcg ggcacgttga gctgtgacgc ccttcactgc 720 tgtcccggac gcctgggtcc tcccctcccc ccgctgcccg ggctacgtct ctctgccgtc 780 ctggatgcct gggtcctgtt ttcataccct gaagcttttc aggtggcaag cagctgtgct 840 cctggtcttg ttgcttg 857 // ID SCPV02062730; SV 1; linear; genomic DNA; STD; VRT; 628 BP. XX AC SCPV02062730; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22372.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-628 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-628 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; c49d4a10d6079e0fffc9679f8f157913. DR ENA-CON; ML191189. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..628 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22372.1" XX SQ Sequence 628 BP; 105 A; 131 C; 266 G; 126 T; 0 other; ctggccaatc tcagccaggt ggagggggag acagtgtgga gggcttgact gggcccctcc 60 aggcaagtgt gtcttgaggg accctggccg ggacaggtcc tggcccggct gatcgtgcca 120 ctcccaaggg gccagagcga ttcccagccc tgggaccggc tctggctgca ctgccaactg 180 agcctggcag acctagttgc ctcccagcag gctggtgagt gcggccagaa ggcagggtgt 240 gtgggagtgg gtctcttgga gaattggggg gggggctggg ctgtgagagg gtggggaagg 300 tgtgtgtgtt ggggcactag ggagtgtggg ttcagaggcg gtgctttgca gttgtggtgg 360 ggctgtgggc aggggtggtg ttctgcaggg cactgtgcat ttgtgggtgg gtctcggagg 420 ggcactgggc atagtgggtc cgggggggtt ctggccatag ggagttgggg ggtgttgggc 480 gggggggctg tgtggcgcgg cataggccca cccctgaggg gaaggggcat gctggcaaca 540 cagggctggg caggccactg tgtgtctaga aactgatagt taagatagtt aagaccaaag 600 cagactgtga cgaacttcaa aaagatct 628 // ID SCPV02062731; SV 1; linear; genomic DNA; STD; VRT; 628 BP. XX AC SCPV02062731; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22372.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-628 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-628 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; c49d4a10d6079e0fffc9679f8f157913. DR ENA-CON; ML191189. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..628 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22372.2" XX SQ Sequence 628 BP; 105 A; 131 C; 266 G; 126 T; 0 other; ctggccaatc tcagccaggt ggagggggag acagtgtgga gggcttgact gggcccctcc 60 aggcaagtgt gtcttgaggg accctggccg ggacaggtcc tggcccggct gatcgtgcca 120 ctcccaaggg gccagagcga ttcccagccc tgggaccggc tctggctgca ctgccaactg 180 agcctggcag acctagttgc ctcccagcag gctggtgagt gcggccagaa ggcagggtgt 240 gtgggagtgg gtctcttgga gaattggggg gggggctggg ctgtgagagg gtggggaagg 300 tgtgtgtgtt ggggcactag ggagtgtggg ttcagaggcg gtgctttgca gttgtggtgg 360 ggctgtgggc aggggtggtg ttctgcaggg cactgtgcat ttgtgggtgg gtctcggagg 420 ggcactgggc atagtgggtc cgggggggtt ctggccatag ggagttgggg ggtgttgggc 480 gggggggctg tgtggcgcgg cataggccca cccctgaggg gaaggggcat gctggcaaca 540 cagggctggg caggccactg tgtgtctaga aactgatagt taagatagtt aagaccaaag 600 cagactgtga cgaacttcaa aaagatct 628 // ID SCPV02062732; SV 1; linear; genomic DNA; STD; VRT; 799 BP. XX AC SCPV02062732; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22373.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-799 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-799 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e0628fa436164442c9c7c3dd393c3e06. DR ENA-CON; ML191190. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..799 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22373.1" XX SQ Sequence 799 BP; 120 A; 279 C; 301 G; 99 T; 0 other; cctgccctct gactgcccca acactaatcc accccccaac tcccctgccc tctatccaac 60 accctctccc tgctccctta ccacgctgcc tgggtggggg ccgagctggg gctgggaccg 120 gagccgcccg gccgaggtcg gggctggacc cgggggctgg gcaggagccg tgctgcgccg 180 cccggccaag gtcgcagccg gacccgggca ggagctgcac cgcgcctccc agcaaggttg 240 gggctggacc cgggggccgg gcaggagctg tgccgcccgg ccgagttcgc agccagaccc 300 gggggctggg caggagctgt gccgcgctgc ccggcggggt caggaccgga cccgggcagg 360 agctgcgccg cgccgcccgg cgaggttggg accggacccg ggcaggagct gcgccatgcc 420 acccagtgag gtcgggaccg gacccggggg ctgggcagga gctgtgccgc ccggcgaggt 480 tgggaccgga cccaggggcc gggcaggagt cacgtcgcct ggcccccggg tagggctgca 540 acctcgccgg gcagtgcagc gcctggagcc ctcctcctgc tgctgccccc gccccccagc 600 cccaactcac ctggtggaag ccgctgcttc cttctcagcc ctcccaggct tcccgcgcga 660 acagctgatt cgcgggaagc tggggaaggg gaggagaagc cgaaggagcg ttcagaagag 720 gaggcagagg cggagtgagg tgagctaggg ccgggagaag ggcagggagc tgctagagct 780 tttgttaaat ttaaaagcc 799 // ID SCPV02062733; SV 1; linear; genomic DNA; STD; VRT; 799 BP. XX AC SCPV02062733; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22373.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-799 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-799 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e0628fa436164442c9c7c3dd393c3e06. DR ENA-CON; ML191190. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..799 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22373.2" XX SQ Sequence 799 BP; 120 A; 279 C; 301 G; 99 T; 0 other; cctgccctct gactgcccca acactaatcc accccccaac tcccctgccc tctatccaac 60 accctctccc tgctccctta ccacgctgcc tgggtggggg ccgagctggg gctgggaccg 120 gagccgcccg gccgaggtcg gggctggacc cgggggctgg gcaggagccg tgctgcgccg 180 cccggccaag gtcgcagccg gacccgggca ggagctgcac cgcgcctccc agcaaggttg 240 gggctggacc cgggggccgg gcaggagctg tgccgcccgg ccgagttcgc agccagaccc 300 gggggctggg caggagctgt gccgcgctgc ccggcggggt caggaccgga cccgggcagg 360 agctgcgccg cgccgcccgg cgaggttggg accggacccg ggcaggagct gcgccatgcc 420 acccagtgag gtcgggaccg gacccggggg ctgggcagga gctgtgccgc ccggcgaggt 480 tgggaccgga cccaggggcc gggcaggagt cacgtcgcct ggcccccggg tagggctgca 540 acctcgccgg gcagtgcagc gcctggagcc ctcctcctgc tgctgccccc gccccccagc 600 cccaactcac ctggtggaag ccgctgcttc cttctcagcc ctcccaggct tcccgcgcga 660 acagctgatt cgcgggaagc tggggaaggg gaggagaagc cgaaggagcg ttcagaagag 720 gaggcagagg cggagtgagg tgagctaggg ccgggagaag ggcagggagc tgctagagct 780 tttgttaaat ttaaaagcc 799 // ID SCPV02062734; SV 1; linear; genomic DNA; STD; VRT; 706 BP. XX AC SCPV02062734; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22374.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-706 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-706 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f18bb797663c9ac7481a4cb0f8bbd8cd. DR ENA-CON; ML191191. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..706 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22374.1" XX SQ Sequence 706 BP; 179 A; 150 C; 134 G; 243 T; 0 other; tatatgtatg tggggggagg gacgtgcaga actggaggga gaaaaggtaa caggggaatg 60 tagttataaa aattttgaaa atgaaaaaaa tatttcttca ctcgtctatg atcctctttc 120 tcagttccat tttctggttc ctctttatct ttccagtcac ttaaaagagt ctctccttag 180 gtttaactac tttggtgatc ccagccagtc tcatagcttt aaacacatat ctgtgttggc 240 aattcccttt tcttttcttt ctttcttttt tttttttttt tttttttttt ttttccatgc 300 aatgtctgca accaaactta tccctaggtc ccagggctgg ggacacaggg cccatgagca 360 gcagaacttg ctgtggcagc catttgggcc agctcccatc atgagaggat aggaactgaa 420 tttgacttga ctgcaggaat tcccaatttt ttatctctgg ctttaggcct ctcccctcga 480 cacacaagtc tgtcttataa tgaagttacc taacaggctt cttggctaac caaagttgat 540 acatccaaag tcaagactac tctgtgagtc cttcacatgt tcaaacagaa tgatttttct 600 tactgttagt gtctcttttg catgggtcat acatcccaga tacacagcag actctataaa 660 ttgtacactg gatgaaagat ttgaacactg gtttcagctg aactgt 706 // ID SCPV02062735; SV 1; linear; genomic DNA; STD; VRT; 706 BP. XX AC SCPV02062735; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22374.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-706 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-706 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f18bb797663c9ac7481a4cb0f8bbd8cd. DR ENA-CON; ML191191. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..706 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22374.2" XX SQ Sequence 706 BP; 179 A; 150 C; 134 G; 243 T; 0 other; tatatgtatg tggggggagg gacgtgcaga actggaggga gaaaaggtaa caggggaatg 60 tagttataaa aattttgaaa atgaaaaaaa tatttcttca ctcgtctatg atcctctttc 120 tcagttccat tttctggttc ctctttatct ttccagtcac ttaaaagagt ctctccttag 180 gtttaactac tttggtgatc ccagccagtc tcatagcttt aaacacatat ctgtgttggc 240 aattcccttt tcttttcttt ctttcttttt tttttttttt tttttttttt ttttccatgc 300 aatgtctgca accaaactta tccctaggtc ccagggctgg ggacacaggg cccatgagca 360 gcagaacttg ctgtggcagc catttgggcc agctcccatc atgagaggat aggaactgaa 420 tttgacttga ctgcaggaat tcccaatttt ttatctctgg ctttaggcct ctcccctcga 480 cacacaagtc tgtcttataa tgaagttacc taacaggctt cttggctaac caaagttgat 540 acatccaaag tcaagactac tctgtgagtc cttcacatgt tcaaacagaa tgatttttct 600 tactgttagt gtctcttttg catgggtcat acatcccaga tacacagcag actctataaa 660 ttgtacactg gatgaaagat ttgaacactg gtttcagctg aactgt 706 // ID SCPV02062736; SV 1; linear; genomic DNA; STD; VRT; 944 BP. XX AC SCPV02062736; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22375.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-944 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-944 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 97c25b9080e2a6e12a1f875d399db854. DR ENA-CON; ML191192. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..944 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22375.1" XX SQ Sequence 944 BP; 151 A; 324 C; 318 G; 151 T; 0 other; aaagaacaga gtttctcttt aaaaagttaa agttatgctt cagtcatacc agccagcatt 60 tctgccacca cagctagttc acatccagtg gtgagctgca gccggttcgc accagttcac 120 gcgaaccggt tgttaaattt agaaaccctt ttagaaccgg ttgttaaatt ttaaagccgt 180 ttaacaaagg ttccagcagc tccctgccct gcccccagtc ccagctcacc tggctccagc 240 tttgtctcct cctctgaagg ctctccctcc aggcttcccg gcaatcagct gttagggcag 300 gaagcctggg agggctgaga aggaagcagc agcagcttcc tgcaggtaag ctgggtgggg 360 tgcgggggag gtgctgcgcg tccccggcca ggcggcgcag ctccgggccg gcccccggct 420 cggaccctga ccaggcccga ctctggccgg gcgggcagcg cggctccggg ccagccccca 480 gccccgactc tgggcggcgc ggctctgggc cggaccccga tgccaacccg gaccccggcc 540 ccgactctgg ccaggcgggc gcagctctga ccccggccgg gcgggtggag cggctgccgg 600 cgcggctctg ggccagaccc cgacgccgac ccggaccctg gccccgactc tggccgggcg 660 ggcggcccgg ctccgggcgg gcggcacagc tctgaccccg gccccaattc tgggcagcgc 720 gcctctgcgc cggctcccag ctctgactct ggctggcggg cgaggctccg ggccggaccc 780 agacgccgac ctggaccccg actgcagccc agcgcctgtg gctcccagcc ccgactctgg 840 gtggcacggc tcccatctcc aagcagcagg gtaaggggga cagggagcga gtgttggata 900 gagggcgggg ggtggattag ggtcgggact gggggtggtc agag 944 // ID SCPV02062737; SV 1; linear; genomic DNA; STD; VRT; 961 BP. XX AC SCPV02062737; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22375.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-961 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-961 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 15f3e94552abdc7f19646457a52d021f. DR ENA-CON; ML191192. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..961 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22375.2" XX SQ Sequence 961 BP; 218 A; 199 C; 340 G; 204 T; 0 other; ggggtcaccc ttgtggggtg aggaggaggg aaccggttgt taatattttg gcagctcatc 60 actgttcaca cctatgttaa aaatagttcc cttacctggg ggaagttatt ctagaacaaa 120 cacagtctga cagccattat ctgctcagtg ctgtgcacac agggagcaga aatggcaatc 180 aacagcaatg ttaaaacagt tatcaggatt ctaccatcaa cctgtgtgaa taagaatttt 240 tgtctgaaaa tcattataaa actatgctgt ataacagcca gacactttat ataatggatt 300 tataacaggg gtgggcaaat tttgtggccc gagggccaca tctaggtatg gaaattgtac 360 agtgggccat gaacgttcac taaattgggg ttggggtaca caaggggggg gagggttctg 420 gctggaggtg taggctctgg agtggggctg ggaatgagag atttggggtg taggaggatg 480 ctccaggctg ggacagaggg attcaggtgc gggatcaggg ttggtacatg gggggaggaa 540 ggggtgcagg ctctggggtg gggccagaaa caagaagttc agcgagcgga ctccgggccg 600 ggatgagggc ttcgactggg ggtgcaggtt ctgggaatga ggggtttggg gcacaggagg 660 gtgctctggg ctgggactga ggggttcaga gggcaggagg gggatcaggg ctggggcagg 720 gggttgggag aggctcaggg gtgcaggctc caggcggcgc ttatctcgag cagctcccga 780 aagcagcagc atgtcccccc tccggctcct acacagaggt gcggccagat ggttctgcgt 840 gctgccccgt ctacaggcac tgcccttacc gctcccattg gctgcagtgc ccagcaaatg 900 ggagctgtgg gggtggcacc tgcggacagg gcagcgcgca gagccccctg actgccccta 960 a 961 // ID SCPV02062738; SV 1; linear; genomic DNA; STD; VRT; 670 BP. XX AC SCPV02062738; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22376.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-670 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-670 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d51905fd86dd843a0db9612a87c85a36. DR ENA-CON; ML191193. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22376.1" XX SQ Sequence 670 BP; 171 A; 157 C; 143 G; 199 T; 0 other; tttacttatg tgcctaaacc ccagtttgag cctccactgc aagtcacaaa actcctgctg 60 agccctgttg gtgcctaaac tcactcggca tctgtgtttt tcagtaaata gttccctcag 120 cacctgtgtt tctgcctctg gccatgtgca ctgctgcctc gcccaaggca tctcgatgac 180 cgtatcccac ctgagcccca gggaagatca gtgttaggcc acctaactcc tgtgtggggt 240 ctaatctggt aggtggactg aggcctctca ggtgagttca tacagagaaa gttctccctc 300 atcattttta gcccaatgtt tagagcactc acctgggata tgagagacca cagattcaag 360 tccctcttcc acctgatgag gagaagggat ttgaacaggg atctgccacc tctctatggg 420 ttattgaggt tgggcttcct ccatctttcc tgttgaagct gttccactgg ggataaatac 480 ttagagagtc attcttattg taaagaaaaa ctgttctgtt ctaaatgagt atacaggaaa 540 attgatcatt aaggaaagag ttttaataat ccagatattt ccattgataa tgcgagtgga 600 atttgtttag ctaacagtag tttctccttt agtctttcaa ctagagctgg tcgaaattca 660 accacaaaaa 670 // ID SCPV02062739; SV 1; linear; genomic DNA; STD; VRT; 670 BP. XX AC SCPV02062739; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22376.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-670 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-670 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d51905fd86dd843a0db9612a87c85a36. DR ENA-CON; ML191193. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..670 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22376.2" XX SQ Sequence 670 BP; 171 A; 157 C; 143 G; 199 T; 0 other; tttacttatg tgcctaaacc ccagtttgag cctccactgc aagtcacaaa actcctgctg 60 agccctgttg gtgcctaaac tcactcggca tctgtgtttt tcagtaaata gttccctcag 120 cacctgtgtt tctgcctctg gccatgtgca ctgctgcctc gcccaaggca tctcgatgac 180 cgtatcccac ctgagcccca gggaagatca gtgttaggcc acctaactcc tgtgtggggt 240 ctaatctggt aggtggactg aggcctctca ggtgagttca tacagagaaa gttctccctc 300 atcattttta gcccaatgtt tagagcactc acctgggata tgagagacca cagattcaag 360 tccctcttcc acctgatgag gagaagggat ttgaacaggg atctgccacc tctctatggg 420 ttattgaggt tgggcttcct ccatctttcc tgttgaagct gttccactgg ggataaatac 480 ttagagagtc attcttattg taaagaaaaa ctgttctgtt ctaaatgagt atacaggaaa 540 attgatcatt aaggaaagag ttttaataat ccagatattt ccattgataa tgcgagtgga 600 atttgtttag ctaacagtag tttctccttt agtctttcaa ctagagctgg tcgaaattca 660 accacaaaaa 670 // ID SCPV02062740; SV 1; linear; genomic DNA; STD; VRT; 671 BP. XX AC SCPV02062740; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22377.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-671 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-671 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 91029f87d765eb171c0bec85327fa5f5. DR ENA-CON; ML191194. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..671 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22377.1" XX SQ Sequence 671 BP; 244 A; 96 C; 117 G; 214 T; 0 other; gccagattgt tcagtttgta tagaatagac tctgccagca atttcaatac agcacctaga 60 agtacatgtt gactgtaaag attcttgcat ataaaaacaa aagtgaacaa aatcagagtg 120 ctcgggtttt tgttttgttt gtgttttttt ttattttgat tttgtttttg tttttttttt 180 taacaatact gatttaacaa gtataagccc agagaaaatt gtgactggag ataacaaaag 240 cccaagactg gagctctaaa agtggccctt agactaggta agaaatattt ctttaaaata 300 tgtttcaatg aagactagtg agtgcctcaa gttcttatca gaaccaaact atcacaagct 360 cacaccagaa agcaaaaaat acagtagaag ctttttgacc acagaaagac ttcaaaacat 420 gattatgtga agaactttaa aagtgataaa tttttagggg ttaaggggca cttacaaatt 480 tcgtactggc cctattacaa atgatctaag ctttttggta atgaagcagc agtgtcagtt 540 caatcaatta caaaacatag ctcatgggaa taaggattat atttaacgga ggtcttagaa 600 taaagttaat atttatggaa gttttaataa tcaaacatca atgcttaaaa aaatcacatt 660 aaatataagg t 671 // ID SCPV02062741; SV 1; linear; genomic DNA; STD; VRT; 671 BP. XX AC SCPV02062741; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22377.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-671 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-671 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 91029f87d765eb171c0bec85327fa5f5. DR ENA-CON; ML191194. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..671 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22377.2" XX SQ Sequence 671 BP; 244 A; 96 C; 117 G; 214 T; 0 other; gccagattgt tcagtttgta tagaatagac tctgccagca atttcaatac agcacctaga 60 agtacatgtt gactgtaaag attcttgcat ataaaaacaa aagtgaacaa aatcagagtg 120 ctcgggtttt tgttttgttt gtgttttttt ttattttgat tttgtttttg tttttttttt 180 taacaatact gatttaacaa gtataagccc agagaaaatt gtgactggag ataacaaaag 240 cccaagactg gagctctaaa agtggccctt agactaggta agaaatattt ctttaaaata 300 tgtttcaatg aagactagtg agtgcctcaa gttcttatca gaaccaaact atcacaagct 360 cacaccagaa agcaaaaaat acagtagaag ctttttgacc acagaaagac ttcaaaacat 420 gattatgtga agaactttaa aagtgataaa tttttagggg ttaaggggca cttacaaatt 480 tcgtactggc cctattacaa atgatctaag ctttttggta atgaagcagc agtgtcagtt 540 caatcaatta caaaacatag ctcatgggaa taaggattat atttaacgga ggtcttagaa 600 taaagttaat atttatggaa gttttaataa tcaaacatca atgcttaaaa aaatcacatt 660 aaatataagg t 671 // ID SCPV02062742; SV 1; linear; genomic DNA; STD; VRT; 507 BP. XX AC SCPV02062742; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22378.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-507 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-507 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 55f9c050d1d38b5616d4d7992d4af8cf. DR ENA-CON; ML191195. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..507 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22378.1" XX SQ Sequence 507 BP; 82 A; 99 C; 205 G; 121 T; 0 other; tacacctgga agtgggattg gcatgccagg caggggcaca cggaacggag gcaccattac 60 tggtgggaaa gcaggtattg ctgctgccgg ctgaggtacc gagtgaggaa cagcaggagg 120 taacgtctgt gggtgcggct ggggtacggt ttgcacaggt gtggctgtag gagcaggagc 180 tgaaacacta actgtaggtg tggcaactga aacagcagaa cttggggtct ggtcttgtgt 240 tgtgggtgtt gatactgtct gtgcaactga agtagcagtt gtcgtggtag ctgtggtgga 300 ggatggggta gatgtcggag ttgacgtgga cactgcaggt gctgggctac tggtggtagg 360 ggtgggagcg cctactgctt gtgcttggac ttgtgcctgg gcctgagcct gggcctgggc 420 ttgggcctgg gcttgtgctt gcgcttgggc ttgggcttga gcctgagcct gggcctgggc 480 ctgggcctga gcctgggctt gtgcctg 507 // ID SCPV02062743; SV 1; linear; genomic DNA; STD; VRT; 507 BP. XX AC SCPV02062743; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22378.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-507 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-507 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 55f9c050d1d38b5616d4d7992d4af8cf. DR ENA-CON; ML191195. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..507 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22378.2" XX SQ Sequence 507 BP; 82 A; 99 C; 205 G; 121 T; 0 other; tacacctgga agtgggattg gcatgccagg caggggcaca cggaacggag gcaccattac 60 tggtgggaaa gcaggtattg ctgctgccgg ctgaggtacc gagtgaggaa cagcaggagg 120 taacgtctgt gggtgcggct ggggtacggt ttgcacaggt gtggctgtag gagcaggagc 180 tgaaacacta actgtaggtg tggcaactga aacagcagaa cttggggtct ggtcttgtgt 240 tgtgggtgtt gatactgtct gtgcaactga agtagcagtt gtcgtggtag ctgtggtgga 300 ggatggggta gatgtcggag ttgacgtgga cactgcaggt gctgggctac tggtggtagg 360 ggtgggagcg cctactgctt gtgcttggac ttgtgcctgg gcctgagcct gggcctgggc 420 ttgggcctgg gcttgtgctt gcgcttgggc ttgggcttga gcctgagcct gggcctgggc 480 ctgggcctga gcctgggctt gtgcctg 507 // ID SCPV02062744; SV 1; linear; genomic DNA; STD; VRT; 662 BP. XX AC SCPV02062744; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22379.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-662 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-662 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 60c2b227a8957def5ae774cf9b382e35. DR ENA-CON; ML191196. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..662 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22379.1" XX SQ Sequence 662 BP; 151 A; 165 C; 200 G; 146 T; 0 other; ctttcttata tggggacttc cggagctgtg gggacgactc ttctggagga agcagcgcgg 60 gcttgaccgg cgtcggcccg ccgcctccgc tgccgcttcg ccccaatccg gtccctctgg 120 cccggcctga cccggtctgg cttgttcggg ctcagcggcc gcgaggccgc agctcccgat 180 ggaaatcata ttatgtagaa tacttgggtg acatctgcct gagagatctc caagaattac 240 agattgagtc tcgctctgtt gcccaggctg gagtgcagtg gtgcaatctc tgctcactgc 300 aacctccgtc tcccgagttc aagcagttct cctgcctcag cctcctgagt agctgggatt 360 acagaagaca aatatactaa tgcatttgag aaagcggtag ttttgggggg agggggaaaa 420 agcaactgct ttcctgatct gcaacttggc tggatgctaa gatgtcagtg gacatgaata 480 gccaggggtc tgacagcaat gaagaggact atgacccaaa ttgtgaggaa gaggaagaag 540 aagaagaaga cgaccctggg gacatagagg actattacgt gggagtagcc agcgatgtgg 600 agcagcaggg ggctgatgcc tttgatcccg aggagtacca gttcacttgc ttgacctaca 660 ag 662 // ID SCPV02062745; SV 1; linear; genomic DNA; STD; VRT; 662 BP. XX AC SCPV02062745; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22379.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-662 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-662 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 60c2b227a8957def5ae774cf9b382e35. DR ENA-CON; ML191196. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..662 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22379.2" XX SQ Sequence 662 BP; 151 A; 165 C; 200 G; 146 T; 0 other; ctttcttata tggggacttc cggagctgtg gggacgactc ttctggagga agcagcgcgg 60 gcttgaccgg cgtcggcccg ccgcctccgc tgccgcttcg ccccaatccg gtccctctgg 120 cccggcctga cccggtctgg cttgttcggg ctcagcggcc gcgaggccgc agctcccgat 180 ggaaatcata ttatgtagaa tacttgggtg acatctgcct gagagatctc caagaattac 240 agattgagtc tcgctctgtt gcccaggctg gagtgcagtg gtgcaatctc tgctcactgc 300 aacctccgtc tcccgagttc aagcagttct cctgcctcag cctcctgagt agctgggatt 360 acagaagaca aatatactaa tgcatttgag aaagcggtag ttttgggggg agggggaaaa 420 agcaactgct ttcctgatct gcaacttggc tggatgctaa gatgtcagtg gacatgaata 480 gccaggggtc tgacagcaat gaagaggact atgacccaaa ttgtgaggaa gaggaagaag 540 aagaagaaga cgaccctggg gacatagagg actattacgt gggagtagcc agcgatgtgg 600 agcagcaggg ggctgatgcc tttgatcccg aggagtacca gttcacttgc ttgacctaca 660 ag 662 // ID SCPV02062746; SV 1; linear; genomic DNA; STD; VRT; 895 BP. XX AC SCPV02062746; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22380.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-895 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-895 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 595efdd5ac041a2ae9cab914615cb78c. DR ENA-CON; ML191197. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..895 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22380.1" XX SQ Sequence 895 BP; 204 A; 287 C; 230 G; 174 T; 0 other; gccgccatcc tgctggcgaa agagaggaga gagacagaga ggcccgcccg ctcgccggcc 60 gggccagtgt gggccagtgc gtgagggagg gcgcgggggg ccgtgtcagc gcgacgcaga 120 gcgcagccgc cgccgccaaa cggacatgtt ggagccccgc gctgccgccg cctcccatcc 180 ctcctccggc gcacgggact gagcaggggc ccgccgcctc tgcctgcgct cgaccggccc 240 agcctcgccg cctcggccgc agccccttcc tgctcggcct ccgccgctcg tcgcctcccg 300 tcctcctccc cctccacgcc caagggccct gccggcaccc ggccctactc agccgccgct 360 ccaggtctag gtgaccggcg ggcccggagc agccgcagcc gccgccgcaa cgcgcgcgaa 420 catggacgcc gtcaacgcct tcaaccagga gctcttctca cttatggata tgaagcctcc 480 catctctaga gccaagatga ttctcattac taaagctgct atcaaagcta ttaagcttta 540 taagcacgtt gttcaaatag tagaaaagtt catcaaaaag tgtaagccag aatataaggt 600 tccaggcctg tatgtcatcg actccatcgt gagacagtct cggcaccagt ttggaactga 660 taaagatgtt tttgggccaa gattctctaa aaatataact gctacattcc agtatttata 720 tctttgtcca tctgaagata agagtaaaat agttcgtgtg ctaaatcttt ggcaaaaaaa 780 tggagtgttc aaaattgaaa ttattcagcc tcttttggac atggcagcag gaaccagtaa 840 tgctgctcca gtagcagaaa atgtcaccaa caatgaaggc tcacctccac cccca 895 // ID SCPV02062747; SV 1; linear; genomic DNA; STD; VRT; 895 BP. XX AC SCPV02062747; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22380.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-895 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-895 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 595efdd5ac041a2ae9cab914615cb78c. DR ENA-CON; ML191197. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..895 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22380.2" XX SQ Sequence 895 BP; 204 A; 287 C; 230 G; 174 T; 0 other; gccgccatcc tgctggcgaa agagaggaga gagacagaga ggcccgcccg ctcgccggcc 60 gggccagtgt gggccagtgc gtgagggagg gcgcgggggg ccgtgtcagc gcgacgcaga 120 gcgcagccgc cgccgccaaa cggacatgtt ggagccccgc gctgccgccg cctcccatcc 180 ctcctccggc gcacgggact gagcaggggc ccgccgcctc tgcctgcgct cgaccggccc 240 agcctcgccg cctcggccgc agccccttcc tgctcggcct ccgccgctcg tcgcctcccg 300 tcctcctccc cctccacgcc caagggccct gccggcaccc ggccctactc agccgccgct 360 ccaggtctag gtgaccggcg ggcccggagc agccgcagcc gccgccgcaa cgcgcgcgaa 420 catggacgcc gtcaacgcct tcaaccagga gctcttctca cttatggata tgaagcctcc 480 catctctaga gccaagatga ttctcattac taaagctgct atcaaagcta ttaagcttta 540 taagcacgtt gttcaaatag tagaaaagtt catcaaaaag tgtaagccag aatataaggt 600 tccaggcctg tatgtcatcg actccatcgt gagacagtct cggcaccagt ttggaactga 660 taaagatgtt tttgggccaa gattctctaa aaatataact gctacattcc agtatttata 720 tctttgtcca tctgaagata agagtaaaat agttcgtgtg ctaaatcttt ggcaaaaaaa 780 tggagtgttc aaaattgaaa ttattcagcc tcttttggac atggcagcag gaaccagtaa 840 tgctgctcca gtagcagaaa atgtcaccaa caatgaaggc tcacctccac cccca 895 // ID SCPV02062748; SV 1; linear; genomic DNA; STD; VRT; 758 BP. XX AC SCPV02062748; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22381.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-758 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-758 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2d29a65615554a4e1e1f58a64d6f9077. DR ENA-CON; ML191198. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..758 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22381.1" XX SQ Sequence 758 BP; 152 A; 203 C; 180 G; 223 T; 0 other; atgggatact gggagcgctc cggttctggc atggggatct tgatcatctc tttcctaaac 60 ccatttggca ttccgttggg tttagtcttg atgctgaagc tgccatcggg gttgggaatg 120 tcaacgccaa tgctgaggtg tccgttggcg tggccattct tgtgtgcttc catatggtgc 180 tccatcttca gggtctctat catgtctaag agtcgctgcc cattgtcaga ggagactcgg 240 tacagtgggg gttttttgaa atcctcttgg gttaggttga ttaagtcctg gcctgtgaag 300 tgctccagag gctcacagta ttctggcata gcattctcca gcagccagtc tgccaccttc 360 ttgggtgacc agtaaaccac ttccttcatg gtcctggcag acaacattca gtacccccag 420 cttgctctca gacgtcagca gtcactgttc gtacagggca ggacactatc cctccttggc 480 tggttcatct tgttgggcct catgagcaga gactcatttg gcaagatggt cagggcagtt 540 ttaaaacact tcatgcaacg gtgtccaaac attcgttcgc ctcatttctg aacagaattc 600 ttccttggat tattttaaaa agactctcct catcttggct tcctgcaaga tactagcttc 660 tccgcgtgtt ccagtaggtc tccattaccc caaatgtaag caggctcagc tcctcggcca 720 cacccatagc tggatggtcc cttctcactc accacttc 758 // ID SCPV02062749; SV 1; linear; genomic DNA; STD; VRT; 758 BP. XX AC SCPV02062749; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22381.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-758 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-758 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2d29a65615554a4e1e1f58a64d6f9077. DR ENA-CON; ML191198. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..758 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22381.2" XX SQ Sequence 758 BP; 152 A; 203 C; 180 G; 223 T; 0 other; atgggatact gggagcgctc cggttctggc atggggatct tgatcatctc tttcctaaac 60 ccatttggca ttccgttggg tttagtcttg atgctgaagc tgccatcggg gttgggaatg 120 tcaacgccaa tgctgaggtg tccgttggcg tggccattct tgtgtgcttc catatggtgc 180 tccatcttca gggtctctat catgtctaag agtcgctgcc cattgtcaga ggagactcgg 240 tacagtgggg gttttttgaa atcctcttgg gttaggttga ttaagtcctg gcctgtgaag 300 tgctccagag gctcacagta ttctggcata gcattctcca gcagccagtc tgccaccttc 360 ttgggtgacc agtaaaccac ttccttcatg gtcctggcag acaacattca gtacccccag 420 cttgctctca gacgtcagca gtcactgttc gtacagggca ggacactatc cctccttggc 480 tggttcatct tgttgggcct catgagcaga gactcatttg gcaagatggt cagggcagtt 540 ttaaaacact tcatgcaacg gtgtccaaac attcgttcgc ctcatttctg aacagaattc 600 ttccttggat tattttaaaa agactctcct catcttggct tcctgcaaga tactagcttc 660 tccgcgtgtt ccagtaggtc tccattaccc caaatgtaag caggctcagc tcctcggcca 720 cacccatagc tggatggtcc cttctcactc accacttc 758 // ID SCPV02062750; SV 1; linear; genomic DNA; STD; VRT; 855 BP. XX AC SCPV02062750; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22382.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-855 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-855 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 809d702442e035337aaa7bb60036dbb1. DR ENA-CON; ML191199. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..855 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22382.1" XX SQ Sequence 855 BP; 174 A; 203 C; 273 G; 205 T; 0 other; actcgcgcct taggaagctt gggtgtgtgt ggcgcgctgt cttcccgctc gcgtcaggga 60 cctgcccgac tcagcggctg ccatggcatc agatgaaggc aagcttttcg tgggaggact 120 cagcttcgac accaacgagc aggcgctgga gcaggtcttc tccaagtatg ggcagatctc 180 cgaagtggtg gtggtaaagg acagggagac ccagcgatcc cgaggctttg ggtttgtcac 240 ctttgaaaat atcgatgacg ctaaggacgc catgatggct atgaatggga agtctgtgga 300 cgggcggcag atcagagttg accaggctgg caagtcttct gacaaccggt cccgaggata 360 ccggggtggc tctgctggag gccggggctt tttccgtggg ggacgaagcc ggggccgagg 420 gttctccaga ggaggaggag accggggcta tggaggtggc cgctttgagt cccggagtgg 480 gggttatgga ggctccagag actactatgc cagccggagt cagggtggca gctatggtta 540 tcggagctcg ggagggtcct acagagacag ctatgacagt tatgctacac acaacgagta 600 aaagccctcc gcgtccagat cgtccttcca tggctgtaat taagatgggg gagcttcgct 660 gatcgttgtg tagtgagcac cttgttccca cttttgtagt cctttccgtt ctgacccgtc 720 agcagcctgc ttctgaccca agatggcctc gtgactagac ctgtttttaa ggaagcgctg 780 tctgttttta agtattctta aaaacgtttt ggaaagcact tcagatttta ctgtttacca 840 tgagccatgg gtttt 855 // ID SCPV02062751; SV 1; linear; genomic DNA; STD; VRT; 855 BP. XX AC SCPV02062751; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22382.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-855 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-855 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 809d702442e035337aaa7bb60036dbb1. DR ENA-CON; ML191199. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..855 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22382.2" XX SQ Sequence 855 BP; 174 A; 203 C; 273 G; 205 T; 0 other; actcgcgcct taggaagctt gggtgtgtgt ggcgcgctgt cttcccgctc gcgtcaggga 60 cctgcccgac tcagcggctg ccatggcatc agatgaaggc aagcttttcg tgggaggact 120 cagcttcgac accaacgagc aggcgctgga gcaggtcttc tccaagtatg ggcagatctc 180 cgaagtggtg gtggtaaagg acagggagac ccagcgatcc cgaggctttg ggtttgtcac 240 ctttgaaaat atcgatgacg ctaaggacgc catgatggct atgaatggga agtctgtgga 300 cgggcggcag atcagagttg accaggctgg caagtcttct gacaaccggt cccgaggata 360 ccggggtggc tctgctggag gccggggctt tttccgtggg ggacgaagcc ggggccgagg 420 gttctccaga ggaggaggag accggggcta tggaggtggc cgctttgagt cccggagtgg 480 gggttatgga ggctccagag actactatgc cagccggagt cagggtggca gctatggtta 540 tcggagctcg ggagggtcct acagagacag ctatgacagt tatgctacac acaacgagta 600 aaagccctcc gcgtccagat cgtccttcca tggctgtaat taagatgggg gagcttcgct 660 gatcgttgtg tagtgagcac cttgttccca cttttgtagt cctttccgtt ctgacccgtc 720 agcagcctgc ttctgaccca agatggcctc gtgactagac ctgtttttaa ggaagcgctg 780 tctgttttta agtattctta aaaacgtttt ggaaagcact tcagatttta ctgtttacca 840 tgagccatgg gtttt 855 // ID SCPV02062752; SV 1; linear; genomic DNA; STD; VRT; 979 BP. XX AC SCPV02062752; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22383.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-979 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-979 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e83c7b7f0a5805b2c00b835ab9149de2. DR ENA-CON; ML191200. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..979 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22383.1" XX SQ Sequence 979 BP; 281 A; 191 C; 279 G; 228 T; 0 other; cttgctggaa gctttttcct agaggttgag cggtttgcac aatgtcggaa atggctgagt 60 tgtccgagct gtatgaagag agcagtgacc tgcagatgga tgtgatgcct ggcgagggtg 120 accttccgca gatggaggta ggcagcggga gccgggagct atccctgcgt ccctcccgca 180 gcggggccca acagctcgag gaggaaggcc caatggagga ggaggaggcc cagccaatgg 240 cggcgccaga ggggaaacgg agccttgcta acgggcccaa cgctggggag cagccaggcc 300 aggtggcggg cgcagacttc gagagcgagg acgagggcga ggaatttgat gactgggagg 360 acgactacga ctatcccgaa gaggagcagc tcagtggtgc cggctacaga gtatcagccg 420 ctcttgaaga agccgacaag atgtttctga gaacaagaga accagccctg gatggcgggt 480 ttcagatgca ttatgagaag accccgtttg atcagttagc ttttatcgaa gagctttttt 540 cactgatggt tgtcaatcgt ctgaccgaag aactcggctg tgatgagatt attgatagag 600 agtagttaga tgctgttaaa agaggaggaa actacttgag gagggaccca actttccgct 660 atcttttggg ttcattccaa atagttttgt gccattgaaa aacttgacct tcaaaaaaat 720 ttgtttttca gaatagaaca caataggaca gtgactgcac agttgtgaaa aaggaagaga 780 atcattaaag aaaaagaaaa aagattttaa gaccgttgaa atcaattatc aagaacgtcc 840 taaaacacct atggctttga ctttgttatt gatccagatt attttccttg cattggggaa 900 aatatctttc atatttgttt gctgtaaaga tggttttgca agaataagtc atgaccaaga 960 caaactgcca atacaaaag 979 // ID SCPV02062753; SV 1; linear; genomic DNA; STD; VRT; 979 BP. XX AC SCPV02062753; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22383.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-979 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-979 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e83c7b7f0a5805b2c00b835ab9149de2. DR ENA-CON; ML191200. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..979 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22383.2" XX SQ Sequence 979 BP; 281 A; 191 C; 279 G; 228 T; 0 other; cttgctggaa gctttttcct agaggttgag cggtttgcac aatgtcggaa atggctgagt 60 tgtccgagct gtatgaagag agcagtgacc tgcagatgga tgtgatgcct ggcgagggtg 120 accttccgca gatggaggta ggcagcggga gccgggagct atccctgcgt ccctcccgca 180 gcggggccca acagctcgag gaggaaggcc caatggagga ggaggaggcc cagccaatgg 240 cggcgccaga ggggaaacgg agccttgcta acgggcccaa cgctggggag cagccaggcc 300 aggtggcggg cgcagacttc gagagcgagg acgagggcga ggaatttgat gactgggagg 360 acgactacga ctatcccgaa gaggagcagc tcagtggtgc cggctacaga gtatcagccg 420 ctcttgaaga agccgacaag atgtttctga gaacaagaga accagccctg gatggcgggt 480 ttcagatgca ttatgagaag accccgtttg atcagttagc ttttatcgaa gagctttttt 540 cactgatggt tgtcaatcgt ctgaccgaag aactcggctg tgatgagatt attgatagag 600 agtagttaga tgctgttaaa agaggaggaa actacttgag gagggaccca actttccgct 660 atcttttggg ttcattccaa atagttttgt gccattgaaa aacttgacct tcaaaaaaat 720 ttgtttttca gaatagaaca caataggaca gtgactgcac agttgtgaaa aaggaagaga 780 atcattaaag aaaaagaaaa aagattttaa gaccgttgaa atcaattatc aagaacgtcc 840 taaaacacct atggctttga ctttgttatt gatccagatt attttccttg cattggggaa 900 aatatctttc atatttgttt gctgtaaaga tggttttgca agaataagtc atgaccaaga 960 caaactgcca atacaaaag 979 // ID SCPV02062754; SV 1; linear; genomic DNA; STD; VRT; 693 BP. XX AC SCPV02062754; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22384.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-693 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-693 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d6dac1782bed2618bb8a051c17f73eff. DR ENA-CON; ML191201. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..693 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22384.1" XX SQ Sequence 693 BP; 210 A; 144 C; 157 G; 182 T; 0 other; aatactactg catcaggtgt tgaagacctt aacatcattc aggtgacaat tccagatgat 60 gataatgaaa gactctcgaa agttgaaaaa gctagacagc taagagaaca agtgaatgac 120 ctctttagtc ggaaatttgg tgaagctagt ggtatgggtt ttcctgtgaa agttccctac 180 aggaaaatca caattaaccc tggctgtgtg gtggttgatg gcatgccccc gggggtgtcc 240 ttcaaagccc ccagctacct ggaaatcagc tccatgagaa ggatcttaga ctctgccgag 300 tttatcaaat tcacggtcat tagaccattt ccaggacttg tgattaataa ccagctggtt 360 gatcagagtg agtcagaagg ccccgtgata caagaatcag ctgaaccaag ccagttggaa 420 gttccagcca cagaagaaat aaaagagact gatggaagct ctcagatcaa gcaagaacca 480 gaccccacgt ggtagacctc ttccctccta ggcttaaagt atcagtggtt gagaagagct 540 tttcggacct gttactaccc caagctgtgt aatatacttg tataacagaa ataccttcta 600 tacaaacctt tttttctact tttagataga aatgtctact ttttcagcag ttctgtgaat 660 taaagagcag agtgactgtg ggtctggaat ggc 693 // ID SCPV02062755; SV 1; linear; genomic DNA; STD; VRT; 693 BP. XX AC SCPV02062755; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22384.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-693 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-693 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d6dac1782bed2618bb8a051c17f73eff. DR ENA-CON; ML191201. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..693 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22384.2" XX SQ Sequence 693 BP; 210 A; 144 C; 157 G; 182 T; 0 other; aatactactg catcaggtgt tgaagacctt aacatcattc aggtgacaat tccagatgat 60 gataatgaaa gactctcgaa agttgaaaaa gctagacagc taagagaaca agtgaatgac 120 ctctttagtc ggaaatttgg tgaagctagt ggtatgggtt ttcctgtgaa agttccctac 180 aggaaaatca caattaaccc tggctgtgtg gtggttgatg gcatgccccc gggggtgtcc 240 ttcaaagccc ccagctacct ggaaatcagc tccatgagaa ggatcttaga ctctgccgag 300 tttatcaaat tcacggtcat tagaccattt ccaggacttg tgattaataa ccagctggtt 360 gatcagagtg agtcagaagg ccccgtgata caagaatcag ctgaaccaag ccagttggaa 420 gttccagcca cagaagaaat aaaagagact gatggaagct ctcagatcaa gcaagaacca 480 gaccccacgt ggtagacctc ttccctccta ggcttaaagt atcagtggtt gagaagagct 540 tttcggacct gttactaccc caagctgtgt aatatacttg tataacagaa ataccttcta 600 tacaaacctt tttttctact tttagataga aatgtctact ttttcagcag ttctgtgaat 660 taaagagcag agtgactgtg ggtctggaat ggc 693 // ID SCPV02062756; SV 1; linear; genomic DNA; STD; VRT; 965 BP. XX AC SCPV02062756; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22385.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-965 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-965 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 94d1e43678a61da2ca721c0f686f5635. DR ENA-CON; ML191202. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..965 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22385.1" XX SQ Sequence 965 BP; 346 A; 166 C; 210 G; 243 T; 0 other; cagtgaaaag actagtaaag tctgtgacca aacatccaaa ttttctccaa agaagagtag 60 ttctagttgt aatggagaag aaaaaaagtt agaggagtcc tgttctggtt ctgtaagctc 120 cacttactct cactcagcac tatctgtgcc caaagagatg attaagaaga caacaaaact 180 gattgagact acatccaaca tgaactccag ttacattaag tttctaaaac aggcagcaga 240 taattcagaa atgacttctg ctatgaagtt atgtcaactt aagtctttta aatctgtgtt 300 ggatgatatc aagaaggctc atcttgcatt agaagaagat ttgaattcag agatccaagc 360 tttggatgat gtacacaaag aaaaaaatac caaagacctt aaaagcacag atgctaagtc 420 tgaaacaaaa ttaggaaaag gggaaaaatc ctattctaca gaaaagagag agtttttaaa 480 gttagatgct agatcatcag taaaggccat agatggtgaa gagcagagag cacacaaaag 540 tactagtggg gaacataagg gttcaggtag gaaagatggc tctcagtatg aacccactaa 600 cactcctgag gatttagata tggatattgt gtctgttcct tcctcggttc cagaagacat 660 ttttgatagc cttgagagtg ctatggaagt tcagagttca gctgattatc aaggagacgg 720 caacagtggg actgagccag aactggagag ctcctctgta aaattaaatg tttcttcaaa 780 agacagcaga ggaaatatta agtcaaaagt cacagcgaaa gtaagaaaag aattatttgt 840 taaacttacc ccagtttccc tttctaattc cccaattaaa ggtgttgact gtcaggaagt 900 gtcacaagag aagaatggcc gtaaaagttc tggggtggcc cgcagctcag agaaatgtag 960 accca 965 // ID SCPV02062757; SV 1; linear; genomic DNA; STD; VRT; 1027 BP. XX AC SCPV02062757; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22385.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-1027 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-1027 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 1034096639a805be3aa8d695fd0920c2. DR ENA-CON; ML191202. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1027 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22385.2" XX SQ Sequence 1027 BP; 391 A; 184 C; 256 G; 196 T; 0 other; ccgtaaaagt tctggggtgg cccgcagctc agagaaatgt agacccagag aggaaattag 60 tgatcatgaa aacaatgtca caatactttt agaagactcc gatcttcgaa gatccccacg 120 tgtaaaaact acaccgttga ggcgacagac agaaagcaac cctgcaatgt cgaattcaga 180 tgaagaaagt aatggaacta tgaaggagaa acaaaagatg tcaggtccaa taagaaaaaa 240 ggataagaga aactcagctg actgtgctac tgataaccct aaacctcaca aggtacccaa 300 agctaagcaa ccagtgattg gggatcagaa ttcagattct gatgaaatgc tagcagtcct 360 caaagaggca agtcagatgg ggcatagttc ttcctcagac actgacatta atgaaccaca 420 gatgaatcat aaaggtaaaa cagggaaaga tgataatgga aaaaggaaac ggaaaaattc 480 tacttccggg tcagattttg acactaaaaa aggaaaatca acagaaacct ctattatctc 540 taaaaagaaa cgccagaact actcagagtc ctctaactat gactcagagt tagagagaga 600 gataaaaacc atgagcagaa ttggggctgc cagaaaaagt gttccagaga aaaaagaaga 660 ggactcttct gaagatgaaa aacagggcaa aaaagtagtg gataatggag ggcatgagag 720 ggcgaagaca acacaagaag ggtcatctgc tgatgacact ggtgacactg aaggcaggca 780 gggagggagt tgctccatag caggaggttc cattgagaaa gtcaggagtg gagtggaatt 840 ccgagagatg ctttgcaaac ctggtgtttc ctctgatggt gctgaaaagc cttcagtgaa 900 agaggagaac gtgaattccc ctgaagacaa aagagtctct aagactaaag aaaagactaa 960 acatctgaga agcagacaaa gtagaaaagg aaagggtggg tcatctgatg gcactgatcg 1020 atttcca 1027 // ID SCPV02062758; SV 1; linear; genomic DNA; STD; VRT; 708 BP. XX AC SCPV02062758; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22386.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-708 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-708 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 70b07eb987390ad96e0137f4c99c2c2d. DR ENA-CON; ML191203. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..708 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22386.1" XX SQ Sequence 708 BP; 178 A; 185 C; 193 G; 152 T; 0 other; cttacactgt taccatcaag tatggcggcc agcctgtgcc caacttcccc agcaagctac 60 aggtggaacc tgctgtagat acctcaggtg tacagtgcta tgggcctggg attgaaggtc 120 aaggtgtctt ccgagaggca accactgagt tcagtgtgga tgcccgggct cttacacaga 180 ctggagggcc acatgtcaag gctcgagtgg ccaacccctc aggcaatctg acagatacct 240 atgtgcaaga ctgtggggat ggcacataca aagtggaata caccccatat aagaaaggag 300 tacactctgt ggatgtgact tatgatggca gccctgtgcc cagcagcccc ttccaggtgc 360 ctgtaacaga gggctgtgac ccctcccggg tgcgtgtcca tggaccaggc atccaaagtg 420 gtaccaccaa caaacccaac aagttcacag tagaaaccag gggagctggc acaggtggcc 480 tgggcttggc tgttgagggt ccctcagagg ccaagatgtc ttgtatggat aataaagatg 540 gcagctgctc agtagaatac atcccctatg aagctggaac ctatagcctt aatgtcactt 600 atggtggtca ccaagtgcca ggtagtccct tcaaggtccc tgtacatgat gtgacagatg 660 catctaaagt caagtgttct ggacctggcc taagcccagg catggtcc 708 // ID SCPV02062759; SV 1; linear; genomic DNA; STD; VRT; 708 BP. XX AC SCPV02062759; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22386.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-708 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-708 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 70b07eb987390ad96e0137f4c99c2c2d. DR ENA-CON; ML191203. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..708 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22386.2" XX SQ Sequence 708 BP; 178 A; 185 C; 193 G; 152 T; 0 other; cttacactgt taccatcaag tatggcggcc agcctgtgcc caacttcccc agcaagctac 60 aggtggaacc tgctgtagat acctcaggtg tacagtgcta tgggcctggg attgaaggtc 120 aaggtgtctt ccgagaggca accactgagt tcagtgtgga tgcccgggct cttacacaga 180 ctggagggcc acatgtcaag gctcgagtgg ccaacccctc aggcaatctg acagatacct 240 atgtgcaaga ctgtggggat ggcacataca aagtggaata caccccatat aagaaaggag 300 tacactctgt ggatgtgact tatgatggca gccctgtgcc cagcagcccc ttccaggtgc 360 ctgtaacaga gggctgtgac ccctcccggg tgcgtgtcca tggaccaggc atccaaagtg 420 gtaccaccaa caaacccaac aagttcacag tagaaaccag gggagctggc acaggtggcc 480 tgggcttggc tgttgagggt ccctcagagg ccaagatgtc ttgtatggat aataaagatg 540 gcagctgctc agtagaatac atcccctatg aagctggaac ctatagcctt aatgtcactt 600 atggtggtca ccaagtgcca ggtagtccct tcaaggtccc tgtacatgat gtgacagatg 660 catctaaagt caagtgttct ggacctggcc taagcccagg catggtcc 708 // ID SCPV02062760; SV 1; linear; genomic DNA; STD; VRT; 631 BP. XX AC SCPV02062760; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22387.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-631 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-631 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e2e42c47ff26eb9d8bc4503fd1b174c2. DR ENA-CON; ML191204. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..631 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22387.1" XX SQ Sequence 631 BP; 182 A; 120 C; 155 G; 174 T; 0 other; gcagatgggc agggaagaaa gtctgagcag tgaggaaggg ctgcaggtgc agagaaagtg 60 tgtgatcatt ggtggcccta ttactctatc tctgagctgt ttagcagtga ccagttgcac 120 aagcctgtgt gtgaacacat aagctgcata cacccaaggc caccgctgac tgtgccacac 180 acatgcaaag cctgctatac agctctgagg aacacagagg ggttgagaga atgtctgact 240 caatcctgaa gtgagcattg ttcgatgatc atgcgttgaa tctaaccaag tttactgagc 300 tgtaattagg ttatacaaga ataatgacat ggcttgtgca tttaagatgg aaggatgtat 360 ttgtccctgt aggtttcaaa aaagggaaca ttaatactaa ctccttattt aattagtaca 420 gtacctgtag ggtattttcc tgttggccct gaaaattagc cattgtattt tgacattaat 480 cctaatgtgg ctggacatct gggaatgatg taacagtgtc aatcagggct gatgtgtatt 540 gtgacatccc tcttgaatga attgctttaa ggcgtgattc agctgacaat gcacaccggt 600 tcacagacag gtcactcaca aattcagaaa t 631 // ID SCPV02062761; SV 1; linear; genomic DNA; STD; VRT; 631 BP. XX AC SCPV02062761; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22387.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-631 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-631 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e2e42c47ff26eb9d8bc4503fd1b174c2. DR ENA-CON; ML191204. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..631 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22387.2" XX SQ Sequence 631 BP; 182 A; 120 C; 155 G; 174 T; 0 other; gcagatgggc agggaagaaa gtctgagcag tgaggaaggg ctgcaggtgc agagaaagtg 60 tgtgatcatt ggtggcccta ttactctatc tctgagctgt ttagcagtga ccagttgcac 120 aagcctgtgt gtgaacacat aagctgcata cacccaaggc caccgctgac tgtgccacac 180 acatgcaaag cctgctatac agctctgagg aacacagagg ggttgagaga atgtctgact 240 caatcctgaa gtgagcattg ttcgatgatc atgcgttgaa tctaaccaag tttactgagc 300 tgtaattagg ttatacaaga ataatgacat ggcttgtgca tttaagatgg aaggatgtat 360 ttgtccctgt aggtttcaaa aaagggaaca ttaatactaa ctccttattt aattagtaca 420 gtacctgtag ggtattttcc tgttggccct gaaaattagc cattgtattt tgacattaat 480 cctaatgtgg ctggacatct gggaatgatg taacagtgtc aatcagggct gatgtgtatt 540 gtgacatccc tcttgaatga attgctttaa ggcgtgattc agctgacaat gcacaccggt 600 tcacagacag gtcactcaca aattcagaaa t 631 // ID SCPV02062762; SV 1; linear; genomic DNA; STD; VRT; 608 BP. XX AC SCPV02062762; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22388.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-608 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-608 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2d4aa0ccd38059a9d072d99732e00f0a. DR ENA-CON; ML191205. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..608 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22388.1" XX SQ Sequence 608 BP; 175 A; 133 C; 156 G; 144 T; 0 other; ggccgaggcg cggtgtatgc tgagccgctg ccgcagcggg ctgctccacg tcctgggcct 60 tagcttcctg ctgcagaccc gccggccgat tctcctctgc tctccacgtc tcatgaagcc 120 gctggtcgtg ttcgtcctcg gcggccccgg cgccggcaag gggacccagt gcgcccgcat 180 cgtcgagaaa tatggctaca cacacctttc tgcaggagag ctgcttcgtg atgaaaggaa 240 gaacccagat tcacattatg gtgaacttat tgaaaagtac attaaagaag gaaagattgt 300 accagttgag ataaccatca gtttattaaa gagggaaatg gaccaaacaa tggctgccaa 360 tgctcagaag aataaattct tgattgatgg atttccaaga aatcaagaca accttcaagg 420 ttggaacaaa accatggatg gaaaagcaga tgtatctttt gttctgtttt ttgactgtaa 480 taatgagatc tgtattgaac gatgtcttga aaggggaaaa agtagtggta ggagtgacga 540 caacagagag agcttggaaa agagaattca gacttacctt gaatcaacga aaccaattat 600 tgacttat 608 // ID SCPV02062763; SV 1; linear; genomic DNA; STD; VRT; 608 BP. XX AC SCPV02062763; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22388.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-608 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-608 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2d4aa0ccd38059a9d072d99732e00f0a. DR ENA-CON; ML191205. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..608 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22388.2" XX SQ Sequence 608 BP; 175 A; 133 C; 156 G; 144 T; 0 other; ggccgaggcg cggtgtatgc tgagccgctg ccgcagcggg ctgctccacg tcctgggcct 60 tagcttcctg ctgcagaccc gccggccgat tctcctctgc tctccacgtc tcatgaagcc 120 gctggtcgtg ttcgtcctcg gcggccccgg cgccggcaag gggacccagt gcgcccgcat 180 cgtcgagaaa tatggctaca cacacctttc tgcaggagag ctgcttcgtg atgaaaggaa 240 gaacccagat tcacattatg gtgaacttat tgaaaagtac attaaagaag gaaagattgt 300 accagttgag ataaccatca gtttattaaa gagggaaatg gaccaaacaa tggctgccaa 360 tgctcagaag aataaattct tgattgatgg atttccaaga aatcaagaca accttcaagg 420 ttggaacaaa accatggatg gaaaagcaga tgtatctttt gttctgtttt ttgactgtaa 480 taatgagatc tgtattgaac gatgtcttga aaggggaaaa agtagtggta ggagtgacga 540 caacagagag agcttggaaa agagaattca gacttacctt gaatcaacga aaccaattat 600 tgacttat 608 // ID SCPV02062764; SV 1; linear; genomic DNA; STD; VRT; 755 BP. XX AC SCPV02062764; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22389.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-755 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-755 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 313f4c0443c0ff78edddfbcd50f2281b. DR ENA-CON; ML191206. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..755 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22389.1" XX SQ Sequence 755 BP; 228 A; 133 C; 184 G; 210 T; 0 other; ggagatagca tcttttccaa tttataaagt gcttttctgt gcacgtgggc atgatgggac 60 agccgagagc aattgctttg catttacaga gagttctcat ggctcagaag aatttcagat 120 acatgttttc tcctgtgaaa ttaaagaggc agtaagcaga attttatata gtttctgcac 180 tgcattcaaa cgttcttcca gacaagtgtc tgatgttaaa gactcagtca tcccgactcc 240 cgacagtgat gtgtttacct tcagtgtttc cttggaggtc aaagaagatg atggaaaagg 300 aaactttagt cccgtgccta aggatagaga taaattttat ttcaaaataa agcaaggaat 360 agagaagaag gttgtgatta cagttcagca actgtctaac aaagaattag ctattgagag 420 atgttttgga atgttattaa gcccaggtcg aaacgtgaag aacagtgaca tgcatttact 480 ggatatggag tccatgggaa agagctatga tgggagagct tacgtcatca cgggcatgtg 540 gaaccccaac gcaccaatat ttctggctct taatgaagaa acgcccaaag ataaacgggt 600 gtacatgacc gttgcagtgg acatggtggt cacagaggtg gtggagccag tccgttttct 660 cctggagaca gtagttcgtg tataccctgc aaatgagaga ttttggtatt tcagcagaaa 720 gactttcaca gagactttct tcatgaggtt gaaac 755 // ID SCPV02062765; SV 1; linear; genomic DNA; STD; VRT; 755 BP. XX AC SCPV02062765; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22389.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-755 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-755 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 313f4c0443c0ff78edddfbcd50f2281b. DR ENA-CON; ML191206. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..755 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22389.2" XX SQ Sequence 755 BP; 228 A; 133 C; 184 G; 210 T; 0 other; ggagatagca tcttttccaa tttataaagt gcttttctgt gcacgtgggc atgatgggac 60 agccgagagc aattgctttg catttacaga gagttctcat ggctcagaag aatttcagat 120 acatgttttc tcctgtgaaa ttaaagaggc agtaagcaga attttatata gtttctgcac 180 tgcattcaaa cgttcttcca gacaagtgtc tgatgttaaa gactcagtca tcccgactcc 240 cgacagtgat gtgtttacct tcagtgtttc cttggaggtc aaagaagatg atggaaaagg 300 aaactttagt cccgtgccta aggatagaga taaattttat ttcaaaataa agcaaggaat 360 agagaagaag gttgtgatta cagttcagca actgtctaac aaagaattag ctattgagag 420 atgttttgga atgttattaa gcccaggtcg aaacgtgaag aacagtgaca tgcatttact 480 ggatatggag tccatgggaa agagctatga tgggagagct tacgtcatca cgggcatgtg 540 gaaccccaac gcaccaatat ttctggctct taatgaagaa acgcccaaag ataaacgggt 600 gtacatgacc gttgcagtgg acatggtggt cacagaggtg gtggagccag tccgttttct 660 cctggagaca gtagttcgtg tataccctgc aaatgagaga ttttggtatt tcagcagaaa 720 gactttcaca gagactttct tcatgaggtt gaaac 755 // ID SCPV02062766; SV 1; linear; genomic DNA; STD; VRT; 897 BP. XX AC SCPV02062766; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22390.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-897 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-897 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 9c0970c30059eed4e2d811f2145f130d. DR ENA-CON; ML191207. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..897 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22390.1" XX SQ Sequence 897 BP; 279 A; 184 C; 183 G; 251 T; 0 other; tttttttttt tttttttttt ttaggggatg ggagacacac aagatcatga acagaaagag 60 aaagaagccc actgattttt gtttggtttt cttttcatca ggatcacaag gccagacaaa 120 gtcgggggac agctcccaca cacggagccc agaagtcttc agtgtggcac cagattcagg 180 gtcacagtca catttgaaag agcatgcttc ctctcacccg gagagcttta tgcctgtttt 240 tgcttttgca ttaactatgg acacagagtg ctaactagct taaataaaaa atgcctccta 300 gtctcttaga gctacaaatt tctaattaaa aacagggtta atttacctag cagataaaag 360 ttacacagat aggaatgagc catgacctct ctccctgtag gaaatggcca taattttctg 420 gtagcacagt tcattgaagg ctgatagaaa tgaccccaaa ggcactggaa atagtatctt 480 gcccagtgga aaagccatac cctaaatttt gggtggaaat ctaatcttct aaactcttct 540 agaacctagg aaattcctag ccaacaattc atcctctgat tcaatttctt tattcccagt 600 ttctattcca tgaaaaatgt aagtactggg gggaaaaatc acatttaagt ctcttcacta 660 gtgagtgtat aggctcttat cagacgctag agttccacct ggtgtgtgca tgacttcatt 720 taacaggcag tgcaataaag caaagagcaa gtgggccagc aggctgcaca gtatacaagc 780 acttagcatt acacaaaagt cgactggcta taaaagagct gtgagacagg aattcaagat 840 tcactgtgcc aagaaagagt cattctcctg tctggaaaac atggatcaaa gatgctt 897 // ID SCPV02062767; SV 1; linear; genomic DNA; STD; VRT; 897 BP. XX AC SCPV02062767; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22390.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-897 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-897 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 9c0970c30059eed4e2d811f2145f130d. DR ENA-CON; ML191207. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..897 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22390.2" XX SQ Sequence 897 BP; 279 A; 184 C; 183 G; 251 T; 0 other; tttttttttt tttttttttt ttaggggatg ggagacacac aagatcatga acagaaagag 60 aaagaagccc actgattttt gtttggtttt cttttcatca ggatcacaag gccagacaaa 120 gtcgggggac agctcccaca cacggagccc agaagtcttc agtgtggcac cagattcagg 180 gtcacagtca catttgaaag agcatgcttc ctctcacccg gagagcttta tgcctgtttt 240 tgcttttgca ttaactatgg acacagagtg ctaactagct taaataaaaa atgcctccta 300 gtctcttaga gctacaaatt tctaattaaa aacagggtta atttacctag cagataaaag 360 ttacacagat aggaatgagc catgacctct ctccctgtag gaaatggcca taattttctg 420 gtagcacagt tcattgaagg ctgatagaaa tgaccccaaa ggcactggaa atagtatctt 480 gcccagtgga aaagccatac cctaaatttt gggtggaaat ctaatcttct aaactcttct 540 agaacctagg aaattcctag ccaacaattc atcctctgat tcaatttctt tattcccagt 600 ttctattcca tgaaaaatgt aagtactggg gggaaaaatc acatttaagt ctcttcacta 660 gtgagtgtat aggctcttat cagacgctag agttccacct ggtgtgtgca tgacttcatt 720 taacaggcag tgcaataaag caaagagcaa gtgggccagc aggctgcaca gtatacaagc 780 acttagcatt acacaaaagt cgactggcta taaaagagct gtgagacagg aattcaagat 840 tcactgtgcc aagaaagagt cattctcctg tctggaaaac atggatcaaa gatgctt 897 // ID SCPV02062768; SV 1; linear; genomic DNA; STD; VRT; 715 BP. XX AC SCPV02062768; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22391.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-715 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-715 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; af9a853d481b174365bcfaecc0b87504. DR ENA-CON; ML191208. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..715 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22391.1" XX SQ Sequence 715 BP; 117 A; 232 C; 154 G; 212 T; 0 other; catgggactg tctgggataa tctctctctt tcgccctgag gattcctcct ccacatccgt 60 gtcactgtct ctcccagagg cactctggct ttgttctaca aggacctgtg gttgggactt 120 gacctcttct gcgcctgggt ctctactcca caaaccaatt cctctctctt tcagatgggc 180 caaagtgagt gctgctgaga cctcctcctc ctcctcctcc tcctcctcat ctgtatcact 240 gttgatcacc atgggtgtgt ggtttctctc tagagggaca tccagtgcag tcttgtcctc 300 cacatctgtg ccactaccag ctaggcagtc ctgcagatgt gccacaccag gtgcttcagg 360 atctcctata ccaactgcaa gtagcacttg gtttttcttc acaggggcta cagggggggt 420 cacagggatc ctctcttctt ccacatctgt ctcactgtcc gcaaacccag gtttgtgatc 480 ttcgtccact tctgtgtcac tgtcctctcc aagagtgggg ctcctctcca gaactgaccc 540 atctgagacc cctgccctgc ctgcctcctt cttgactttt gtgtccttca atttgtgctc 600 agtaggctga gcctgtatgc cagctgtcgt tccgttagct tcaggctgct cagcttcccc 660 tgcagcacca ctgctgtcag ctaaagagga ctcctctact cctggttgct gacct 715 // ID SCPV02062769; SV 1; linear; genomic DNA; STD; VRT; 715 BP. XX AC SCPV02062769; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22391.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-715 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-715 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; af9a853d481b174365bcfaecc0b87504. DR ENA-CON; ML191208. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..715 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22391.2" XX SQ Sequence 715 BP; 117 A; 232 C; 154 G; 212 T; 0 other; catgggactg tctgggataa tctctctctt tcgccctgag gattcctcct ccacatccgt 60 gtcactgtct ctcccagagg cactctggct ttgttctaca aggacctgtg gttgggactt 120 gacctcttct gcgcctgggt ctctactcca caaaccaatt cctctctctt tcagatgggc 180 caaagtgagt gctgctgaga cctcctcctc ctcctcctcc tcctcctcat ctgtatcact 240 gttgatcacc atgggtgtgt ggtttctctc tagagggaca tccagtgcag tcttgtcctc 300 cacatctgtg ccactaccag ctaggcagtc ctgcagatgt gccacaccag gtgcttcagg 360 atctcctata ccaactgcaa gtagcacttg gtttttcttc acaggggcta cagggggggt 420 cacagggatc ctctcttctt ccacatctgt ctcactgtcc gcaaacccag gtttgtgatc 480 ttcgtccact tctgtgtcac tgtcctctcc aagagtgggg ctcctctcca gaactgaccc 540 atctgagacc cctgccctgc ctgcctcctt cttgactttt gtgtccttca atttgtgctc 600 agtaggctga gcctgtatgc cagctgtcgt tccgttagct tcaggctgct cagcttcccc 660 tgcagcacca ctgctgtcag ctaaagagga ctcctctact cctggttgct gacct 715 // ID SCPV02062770; SV 1; linear; genomic DNA; STD; VRT; 785 BP. XX AC SCPV02062770; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22392.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-785 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-785 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 1a1126779aa9657d325a573558ebee60. DR ENA-CON; ML191209. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..785 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22392.1" XX SQ Sequence 785 BP; 198 A; 210 C; 202 G; 175 T; 0 other; gatgcccaca gaggaacagg tgtcttggct ctcacgagaa tgggcaaaaa gggcagctct 60 gccttctcat gtggtcacca tgctggacaa ttttccaacc aatctgcacc ctatgtctca 120 gctcagtgca gccatcacag ccctcaacag tgaaagcaac ttcgcccggg catatgcaga 180 gggaatgaac cgagctaagt actgggagct catctatgag gactgcatgg acctcattgc 240 caagctgccg tgcgttgcag ccaagatcta ccggaatctg taccgggagg gcagcagtat 300 cggagccatt gactctaggc tagactggtc acacaatttt accaacatgt taggctacac 360 cgaccctcag ttcaccgagc tcatgcgttt gtacctcacc atccatagtg accatgaggg 420 tggtaatgta agtgcccaca caagccattt ggtgggcagc gccctttcag acccttacct 480 gtcctttgca gcagccatga atgggctggc ggggcctcta catggactag caaatcagga 540 ggtgcttgtc tggctgacac agctacagaa ggaagttggc aaagacgtgt cagatgagaa 600 gttacgagac tacatctgga acacactcaa ttcaggacgg gtggtcccag gatacggtca 660 tgcagtactg aggaagactg accctcgcta ttcctgtcag cgagagtttg ctctgaaaca 720 tctgcctaag gatcccatgt tcaagctggt ggctcagctg tacaagattg tgcccaatat 780 cctct 785 // ID SCPV02062771; SV 1; linear; genomic DNA; STD; VRT; 785 BP. XX AC SCPV02062771; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22392.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-785 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-785 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 1a1126779aa9657d325a573558ebee60. DR ENA-CON; ML191209. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..785 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22392.2" XX SQ Sequence 785 BP; 198 A; 210 C; 202 G; 175 T; 0 other; gatgcccaca gaggaacagg tgtcttggct ctcacgagaa tgggcaaaaa gggcagctct 60 gccttctcat gtggtcacca tgctggacaa ttttccaacc aatctgcacc ctatgtctca 120 gctcagtgca gccatcacag ccctcaacag tgaaagcaac ttcgcccggg catatgcaga 180 gggaatgaac cgagctaagt actgggagct catctatgag gactgcatgg acctcattgc 240 caagctgccg tgcgttgcag ccaagatcta ccggaatctg taccgggagg gcagcagtat 300 cggagccatt gactctaggc tagactggtc acacaatttt accaacatgt taggctacac 360 cgaccctcag ttcaccgagc tcatgcgttt gtacctcacc atccatagtg accatgaggg 420 tggtaatgta agtgcccaca caagccattt ggtgggcagc gccctttcag acccttacct 480 gtcctttgca gcagccatga atgggctggc ggggcctcta catggactag caaatcagga 540 ggtgcttgtc tggctgacac agctacagaa ggaagttggc aaagacgtgt cagatgagaa 600 gttacgagac tacatctgga acacactcaa ttcaggacgg gtggtcccag gatacggtca 660 tgcagtactg aggaagactg accctcgcta ttcctgtcag cgagagtttg ctctgaaaca 720 tctgcctaag gatcccatgt tcaagctggt ggctcagctg tacaagattg tgcccaatat 780 cctct 785 // ID SCPV02062772; SV 1; linear; genomic DNA; STD; VRT; 804 BP. XX AC SCPV02062772; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22393.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-804 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-804 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4b1b1194a289592b04eb0a2793145cc7. DR ENA-CON; ML191210. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..804 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22393.1" XX SQ Sequence 804 BP; 187 A; 194 C; 189 G; 234 T; 0 other; tctcagaggc ctctaacccg ggagtccttc catcctcctc cgccggttcc acccagaggt 60 cgctgatccc gcctcctata tctggccaac ttcaggactt ttcagactag caattctcag 120 cctgttgatg aagtcttcat attgagtttt ctgcatgact atggaccttg agagccactg 180 tcactggtga tggtgtagcc ctgctggttt gggtaatcct tgaagacatc attaaggcat 240 gatgaaacct gagatgaagg cgtctttgtt caacgttgcc gaatatattg gctcacattc 300 tagtttgcca aaaggatgaa gatacttacc tctcgtatcc tacagcagga gactaccagg 360 attgatgggc atgttcttat caagtgagcc tactgtagtc aacatccatt atcccataca 420 tacctggcag agcagcctaa tctctggaag tggttataac cctcccagca gaggtaggaa 480 gggacctaac tttcaagtag ttcttccaca tttctatgtt tgtcctgggg ctggtcacat 540 acaacaagct gaaaaacatc ttagaagggg ttaagccaga ctcctgtgcc actgtggcat 600 ttggagtccc ccagacgtgt ggaacaggtg gttctgtcat caccctcttt ctcttgagta 660 tgtacgtgtg tggtgtgcat gtgcaggtgt ctgtctgtct gtgggcatat gtatgcacct 720 ctgtatggag attagaagca atgttggaag tctctgtcac tctccctcta tttactgagg 780 cagagattac tggtacaatc agtc 804 // ID SCPV02062773; SV 1; linear; genomic DNA; STD; VRT; 804 BP. XX AC SCPV02062773; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22393.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-804 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-804 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4b1b1194a289592b04eb0a2793145cc7. DR ENA-CON; ML191210. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..804 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22393.2" XX SQ Sequence 804 BP; 187 A; 194 C; 189 G; 234 T; 0 other; tctcagaggc ctctaacccg ggagtccttc catcctcctc cgccggttcc acccagaggt 60 cgctgatccc gcctcctata tctggccaac ttcaggactt ttcagactag caattctcag 120 cctgttgatg aagtcttcat attgagtttt ctgcatgact atggaccttg agagccactg 180 tcactggtga tggtgtagcc ctgctggttt gggtaatcct tgaagacatc attaaggcat 240 gatgaaacct gagatgaagg cgtctttgtt caacgttgcc gaatatattg gctcacattc 300 tagtttgcca aaaggatgaa gatacttacc tctcgtatcc tacagcagga gactaccagg 360 attgatgggc atgttcttat caagtgagcc tactgtagtc aacatccatt atcccataca 420 tacctggcag agcagcctaa tctctggaag tggttataac cctcccagca gaggtaggaa 480 gggacctaac tttcaagtag ttcttccaca tttctatgtt tgtcctgggg ctggtcacat 540 acaacaagct gaaaaacatc ttagaagggg ttaagccaga ctcctgtgcc actgtggcat 600 ttggagtccc ccagacgtgt ggaacaggtg gttctgtcat caccctcttt ctcttgagta 660 tgtacgtgtg tggtgtgcat gtgcaggtgt ctgtctgtct gtgggcatat gtatgcacct 720 ctgtatggag attagaagca atgttggaag tctctgtcac tctccctcta tttactgagg 780 cagagattac tggtacaatc agtc 804 // ID SCPV02062774; SV 1; linear; genomic DNA; STD; VRT; 641 BP. XX AC SCPV02062774; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22394.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-641 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-641 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e112ff42148157cae7bb023eb592a8e2. DR ENA-CON; ML191211. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..641 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22394.1" XX SQ Sequence 641 BP; 160 A; 169 C; 154 G; 158 T; 0 other; gatctatctg ggcttccttc ttccttaaaa cctctgttta cctcaacact ggcatcctga 60 gctgtatcct gttgatcagg cagtcgggcc agagagccca caatctccag cgaggcttca 120 gggcaagatg aaacatcact gctggacttg ctttcccaga ggccatcctg tttggctgat 180 gctccatcca tggtcagggc agcccagact aagaagactc tgaggagcta caagtaactc 240 tggattaagt ttgttttttg ccattagcaa cagctcagag cttctaagga ggatcttact 300 ctgttttcca ttttcaggtt taatgtctca taaatggctt gaagtcaaac caagcaacca 360 tgattccggt tccggcccaa ccctgcggct ccggtaggac gtggctgtgc cccaaaaagg 420 aaacaacagg gtaccagtct cgggcaaccc cccggaaaca aacggtccgc gaggtcttcg 480 ttccggaaga gaggctgccc tcagcggcca gtagccactt tcaaattggg gttggtatgg 540 atctccggac agattgagat atccttcaga aagtggactt aaaattttgt gtgccttccc 600 ttcagaggaa caacttccag cctaagagac gaaatcagga c 641 // ID SCPV02062775; SV 1; linear; genomic DNA; STD; VRT; 641 BP. XX AC SCPV02062775; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22394.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-641 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-641 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e112ff42148157cae7bb023eb592a8e2. DR ENA-CON; ML191211. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..641 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22394.2" XX SQ Sequence 641 BP; 160 A; 169 C; 154 G; 158 T; 0 other; gatctatctg ggcttccttc ttccttaaaa cctctgttta cctcaacact ggcatcctga 60 gctgtatcct gttgatcagg cagtcgggcc agagagccca caatctccag cgaggcttca 120 gggcaagatg aaacatcact gctggacttg ctttcccaga ggccatcctg tttggctgat 180 gctccatcca tggtcagggc agcccagact aagaagactc tgaggagcta caagtaactc 240 tggattaagt ttgttttttg ccattagcaa cagctcagag cttctaagga ggatcttact 300 ctgttttcca ttttcaggtt taatgtctca taaatggctt gaagtcaaac caagcaacca 360 tgattccggt tccggcccaa ccctgcggct ccggtaggac gtggctgtgc cccaaaaagg 420 aaacaacagg gtaccagtct cgggcaaccc cccggaaaca aacggtccgc gaggtcttcg 480 ttccggaaga gaggctgccc tcagcggcca gtagccactt tcaaattggg gttggtatgg 540 atctccggac agattgagat atccttcaga aagtggactt aaaattttgt gtgccttccc 600 ttcagaggaa caacttccag cctaagagac gaaatcagga c 641 // ID SCPV02062776; SV 1; linear; genomic DNA; STD; VRT; 766 BP. XX AC SCPV02062776; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22395.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-766 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-766 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 479ddd8690bddb23bbf0e97fc6b5bf89. DR ENA-CON; ML191212. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..766 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22395.1" XX SQ Sequence 766 BP; 145 A; 205 C; 169 G; 247 T; 0 other; atctctcgtg ggattctctg tacgcctttt cttctttctt gctcaagtga cgaaaaacac 60 acatggaagc agtccctcct ctgatggctt ttggtctgct tgagaaggtt gctatgtcag 120 gcatagtctg gagaatctgg ttaaatatct cctccgtttc ctcctctgac tgctccgcat 180 ctttcctcct ctttatcgtg acttgaacgt ggtatttctt ctctatccac tgctggatct 240 gtttactctt ggtgtctaaa tcgtgctgtc caatatttga agagaaaacg agctctttcg 300 tcatagttgg tccagttttg ggtttggcct tttcctgttc ccggagcttt agccgctcct 360 ggtggatctg ctctccggtc ataagctggt actccggtgg ctctgagctg gtgttcctct 420 gcaccagcct caggtcctgc ttgttcatga gtctgatcac atctgctcga tgcatcatcc 480 ccaggtccat gcccttctca tccaggacgc ggataatccg ctcactaatc ttccggccgg 540 tgttagagaa agcatccctt cttttctgtt tcctctcacc ctgtgggtct ccagcagtac 600 taaaaccttt tgcagaagtc aggtacaaca gctttggggt ggacgcagac agggacagct 660 gtgctggatc tggcttcaca gcgtgtcttt gcaaacatct tccaattaag ttatggtcaa 720 gctttgtagt ttgtagcatc aacctcatca gaagaacagc catcct 766 // ID SCPV02062777; SV 1; linear; genomic DNA; STD; VRT; 766 BP. XX AC SCPV02062777; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22395.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-766 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-766 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 479ddd8690bddb23bbf0e97fc6b5bf89. DR ENA-CON; ML191212. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..766 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22395.2" XX SQ Sequence 766 BP; 145 A; 205 C; 169 G; 247 T; 0 other; atctctcgtg ggattctctg tacgcctttt cttctttctt gctcaagtga cgaaaaacac 60 acatggaagc agtccctcct ctgatggctt ttggtctgct tgagaaggtt gctatgtcag 120 gcatagtctg gagaatctgg ttaaatatct cctccgtttc ctcctctgac tgctccgcat 180 ctttcctcct ctttatcgtg acttgaacgt ggtatttctt ctctatccac tgctggatct 240 gtttactctt ggtgtctaaa tcgtgctgtc caatatttga agagaaaacg agctctttcg 300 tcatagttgg tccagttttg ggtttggcct tttcctgttc ccggagcttt agccgctcct 360 ggtggatctg ctctccggtc ataagctggt actccggtgg ctctgagctg gtgttcctct 420 gcaccagcct caggtcctgc ttgttcatga gtctgatcac atctgctcga tgcatcatcc 480 ccaggtccat gcccttctca tccaggacgc ggataatccg ctcactaatc ttccggccgg 540 tgttagagaa agcatccctt cttttctgtt tcctctcacc ctgtgggtct ccagcagtac 600 taaaaccttt tgcagaagtc aggtacaaca gctttggggt ggacgcagac agggacagct 660 gtgctggatc tggcttcaca gcgtgtcttt gcaaacatct tccaattaag ttatggtcaa 720 gctttgtagt ttgtagcatc aacctcatca gaagaacagc catcct 766 // ID SCPV02062778; SV 1; linear; genomic DNA; STD; VRT; 933 BP. XX AC SCPV02062778; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22396.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-933 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-933 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 8437ddfd9e00f1db0cb45c031e564ace. DR ENA-CON; ML191213. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..933 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22396.1" XX SQ Sequence 933 BP; 276 A; 163 C; 219 G; 275 T; 0 other; tgtaacagca tgtgcaaggc cagcatagag ctccatccct aaaaggtcca gggacagtaa 60 tctctaaaga gttatctcta gttacagttc tataataact ggaacaaaat atggcaaaat 120 cagactaaag ttcttccttt agtcaaaata gtcaagaagc tgtttgtaat tgatgccagc 180 cagcaaaaca aagggaaaat cagttttcta caatggagct gaataaatat atcaaccaca 240 gtccagggca ggcccccatg tccagtagtt ggccaggttg tatgtatgtg ttcattttat 300 ttgttttctt ttcttactgg ttgagttttt tgtttttgtt ttttttaaga tttatttatt 360 tattatatgt aagtatacac tgtagctgtc ttcagacact ccagaagagg gcatcagatg 420 gttgtgagcc accatgtggt cgctgcgatt tgaacttagg accttccaaa gagcagtcag 480 tgctcttaac cactgaacca cctctccagc ccaagttttg ctttttattt tgttgcttgc 540 tgtcagagag gaaacaaagt tgggtgggtg gggtggaaag gggatctgag gagttaagcg 600 gggtgggggg gatgtcaaag tagattcatg gaaaatgtta aagaaaaatc tgtttattat 660 tatgtatgtg tatataacca tgatgcatgt gtgtatggta catgcacagg tgtatttata 720 catgtatgca cttgtgatga acaccaggca catctagaag cagtttaaca aataattcaa 780 ggaagacaga tctcgagcca agcagtcaag ccctgtgctg tggcaaagcc caatgagaca 840 gctagcagaa gtagggtgag cccattcctg ggagcttcgt taaaaactgt attaggtgca 900 ggaacttaga agcacacatt tcagagagag tct 933 // ID SCPV02062779; SV 1; linear; genomic DNA; STD; VRT; 933 BP. XX AC SCPV02062779; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22396.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-933 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-933 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 8437ddfd9e00f1db0cb45c031e564ace. DR ENA-CON; ML191213. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..933 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22396.2" XX SQ Sequence 933 BP; 276 A; 163 C; 219 G; 275 T; 0 other; tgtaacagca tgtgcaaggc cagcatagag ctccatccct aaaaggtcca gggacagtaa 60 tctctaaaga gttatctcta gttacagttc tataataact ggaacaaaat atggcaaaat 120 cagactaaag ttcttccttt agtcaaaata gtcaagaagc tgtttgtaat tgatgccagc 180 cagcaaaaca aagggaaaat cagttttcta caatggagct gaataaatat atcaaccaca 240 gtccagggca ggcccccatg tccagtagtt ggccaggttg tatgtatgtg ttcattttat 300 ttgttttctt ttcttactgg ttgagttttt tgtttttgtt ttttttaaga tttatttatt 360 tattatatgt aagtatacac tgtagctgtc ttcagacact ccagaagagg gcatcagatg 420 gttgtgagcc accatgtggt cgctgcgatt tgaacttagg accttccaaa gagcagtcag 480 tgctcttaac cactgaacca cctctccagc ccaagttttg ctttttattt tgttgcttgc 540 tgtcagagag gaaacaaagt tgggtgggtg gggtggaaag gggatctgag gagttaagcg 600 gggtgggggg gatgtcaaag tagattcatg gaaaatgtta aagaaaaatc tgtttattat 660 tatgtatgtg tatataacca tgatgcatgt gtgtatggta catgcacagg tgtatttata 720 catgtatgca cttgtgatga acaccaggca catctagaag cagtttaaca aataattcaa 780 ggaagacaga tctcgagcca agcagtcaag ccctgtgctg tggcaaagcc caatgagaca 840 gctagcagaa gtagggtgag cccattcctg ggagcttcgt taaaaactgt attaggtgca 900 ggaacttaga agcacacatt tcagagagag tct 933 // ID SCPV02062780; SV 1; linear; genomic DNA; STD; VRT; 771 BP. XX AC SCPV02062780; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22397.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-771 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-771 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; aa4107144c35acfbcfaa62b107a59ce3. DR ENA-CON; ML191214. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..771 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22397.1" XX SQ Sequence 771 BP; 179 A; 209 C; 202 G; 181 T; 0 other; aggcacagga gggctgtagg gcttcccaga aaactcaggg tctggaacac aaacctctaa 60 ggagagatct tcgctcatct tgaagccaaa gtcacattcg tagtcctccc gggtgcagga 120 gcagttggac acaaccactg gccggtcaaa gtcttcccca ttaaagcacg tggcatgagg 180 ggtcctcctt ttgaaaactg tcttgtgtcc tagcaaacac tcatttcctc gctcatcaga 240 tggggaccac agcttgtagt cgttttctgt gcaaggaacc cccaaggcat cagtggcatt 300 gacctgcagg atgagccagc tgtggacact ctccttgttc gagccgaaga tggtgaagac 360 ggtgctcttc tcaccaggtt ctgtgagaag cccatacaca aacacaggct tctccgagaa 420 gacgaacgtc ttccaggtct ccccttcatt ggtgctgtac ttcagctcgt tggtctccat 480 gccctgggca atcgccatga tgatgcctcc atggtcaccc caagtatagt aatgaggtcc 540 gggaagagcc tctctccacc tggctccagc actgctagag atgtacacat tggtcttgct 600 ggccaagttc tttcccacag agccggtagc aatgatgaga ccgggggctg actccttgga 660 taggataggc atcctccgaa gttggaggtt gagcagttga ctgaggcgct gggccaggtg 720 gagggaacaa ccctgagaaa gctcacaatt gattttctct ccatatcctg t 771 // ID SCPV02062781; SV 1; linear; genomic DNA; STD; VRT; 771 BP. XX AC SCPV02062781; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22397.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-771 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-771 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; aa4107144c35acfbcfaa62b107a59ce3. DR ENA-CON; ML191214. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..771 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22397.2" XX SQ Sequence 771 BP; 179 A; 209 C; 202 G; 181 T; 0 other; aggcacagga gggctgtagg gcttcccaga aaactcaggg tctggaacac aaacctctaa 60 ggagagatct tcgctcatct tgaagccaaa gtcacattcg tagtcctccc gggtgcagga 120 gcagttggac acaaccactg gccggtcaaa gtcttcccca ttaaagcacg tggcatgagg 180 ggtcctcctt ttgaaaactg tcttgtgtcc tagcaaacac tcatttcctc gctcatcaga 240 tggggaccac agcttgtagt cgttttctgt gcaaggaacc cccaaggcat cagtggcatt 300 gacctgcagg atgagccagc tgtggacact ctccttgttc gagccgaaga tggtgaagac 360 ggtgctcttc tcaccaggtt ctgtgagaag cccatacaca aacacaggct tctccgagaa 420 gacgaacgtc ttccaggtct ccccttcatt ggtgctgtac ttcagctcgt tggtctccat 480 gccctgggca atcgccatga tgatgcctcc atggtcaccc caagtatagt aatgaggtcc 540 gggaagagcc tctctccacc tggctccagc actgctagag atgtacacat tggtcttgct 600 ggccaagttc tttcccacag agccggtagc aatgatgaga ccgggggctg actccttgga 660 taggataggc atcctccgaa gttggaggtt gagcagttga ctgaggcgct gggccaggtg 720 gagggaacaa ccctgagaaa gctcacaatt gattttctct ccatatcctg t 771 // ID SCPV02062782; SV 1; linear; genomic DNA; STD; VRT; 649 BP. XX AC SCPV02062782; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22398.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-649 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-649 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; a225f9686577124ea70b6dd0ad897225. DR ENA-CON; ML191215. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..649 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22398.1" XX SQ Sequence 649 BP; 210 A; 151 C; 178 G; 110 T; 0 other; gggggaagtg gctacccggc tggaaggcgt gaggagcatt ctgccttttc acctggaagc 60 acaatgtcat cagaatccag caaaaaacgg aaacccaaag tgatccggag tgatggaacc 120 ccaactgaag ggaaacggaa ccggtctgac acggagcagg aaggcaaata ctacagtgag 180 gaggctgaag ttgacctgcg ggaccctggc agagactatg agttgtacaa atacacttgc 240 caggagctac aaaggctgat ggccgagatc caggacctga agagtaaggg cagcaaggac 300 ctggccattg aaattgaaga acgaaggatc cagagctgtg tgcacttcat gactctaaag 360 aagcttaacc gattagccca catcagatta aagaaaggaa gagaccagac ccacgaggcc 420 aagcagaaag tcgatgccta ccacctacag ctacagaacc tgctctacga agtgatgcac 480 ctgcagaagg agatcaccaa atgtctggag ttcaagtcaa agcatgaaga aattgatctg 540 gtcagcttag aggaatttta ttcagaggct ccaccaagca tcagcaaggc cgagatcacc 600 atgggagacc ctcaccaaca aacattggca cgtttggact gggagctgg 649 // ID SCPV02062783; SV 1; linear; genomic DNA; STD; VRT; 649 BP. XX AC SCPV02062783; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22398.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-649 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-649 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; a225f9686577124ea70b6dd0ad897225. DR ENA-CON; ML191215. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..649 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22398.2" XX SQ Sequence 649 BP; 210 A; 151 C; 178 G; 110 T; 0 other; gggggaagtg gctacccggc tggaaggcgt gaggagcatt ctgccttttc acctggaagc 60 acaatgtcat cagaatccag caaaaaacgg aaacccaaag tgatccggag tgatggaacc 120 ccaactgaag ggaaacggaa ccggtctgac acggagcagg aaggcaaata ctacagtgag 180 gaggctgaag ttgacctgcg ggaccctggc agagactatg agttgtacaa atacacttgc 240 caggagctac aaaggctgat ggccgagatc caggacctga agagtaaggg cagcaaggac 300 ctggccattg aaattgaaga acgaaggatc cagagctgtg tgcacttcat gactctaaag 360 aagcttaacc gattagccca catcagatta aagaaaggaa gagaccagac ccacgaggcc 420 aagcagaaag tcgatgccta ccacctacag ctacagaacc tgctctacga agtgatgcac 480 ctgcagaagg agatcaccaa atgtctggag ttcaagtcaa agcatgaaga aattgatctg 540 gtcagcttag aggaatttta ttcagaggct ccaccaagca tcagcaaggc cgagatcacc 600 atgggagacc ctcaccaaca aacattggca cgtttggact gggagctgg 649 // ID SCPV02062784; SV 1; linear; genomic DNA; STD; VRT; 747 BP. XX AC SCPV02062784; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22399.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-747 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-747 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d7661fd10bd45d2fdcb2254b688f7c1a. DR ENA-CON; ML191216. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..747 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22399.1" XX SQ Sequence 747 BP; 259 A; 143 C; 139 G; 206 T; 0 other; acctaaacat cataaaagca ttatacagca gaacaatggt caacatcagg ttaaatgtag 60 agaaacatga agcaatctca cttataatca gggacaagac aaggctgctc actctctcca 120 catctattca atacagcagt tgaagtttta gctagagcaa ttagacaaca aaaggatgtc 180 aaagggttac aaatgagaaa ggaagaagtc aaggtatcgc tatttgctga tgatatgata 240 gtatgcatag ttgatcacca aaattctaac agagaactcc tacatctgat aaacaacttc 300 agataagtgt ctgaaataaa attaactcag acaaatcaat attcttcctc tacagaatgg 360 ataatctgtt ttttttcttt ctttcttttt ttggtttttt ggacagggtt tctctttata 420 gtcctggctg tcatggaact catggtgtag accagggtag actcgaactc agaaatctgc 480 ctccctctgc ctcccgtgtg ctgggattaa aggcttccac caccatgccc agctaaaagt 540 aagcattttc aacaaatggt catggtctgt atgtagagga attcaaattg atccattttt 600 atctccttgt acaaagctga agtctaagca gatcaaggac ctcaacaaaa aattaggaac 660 actaaatcta atagaagaga aagtgggaaa tagcctagaa cattttggca cagggaaaat 720 ttcctgaaca caacagaaat ggctgag 747 // ID SCPV02062785; SV 1; linear; genomic DNA; STD; VRT; 747 BP. XX AC SCPV02062785; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22399.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-747 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-747 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d7661fd10bd45d2fdcb2254b688f7c1a. DR ENA-CON; ML191216. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..747 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22399.2" XX SQ Sequence 747 BP; 259 A; 143 C; 139 G; 206 T; 0 other; acctaaacat cataaaagca ttatacagca gaacaatggt caacatcagg ttaaatgtag 60 agaaacatga agcaatctca cttataatca gggacaagac aaggctgctc actctctcca 120 catctattca atacagcagt tgaagtttta gctagagcaa ttagacaaca aaaggatgtc 180 aaagggttac aaatgagaaa ggaagaagtc aaggtatcgc tatttgctga tgatatgata 240 gtatgcatag ttgatcacca aaattctaac agagaactcc tacatctgat aaacaacttc 300 agataagtgt ctgaaataaa attaactcag acaaatcaat attcttcctc tacagaatgg 360 ataatctgtt ttttttcttt ctttcttttt ttggtttttt ggacagggtt tctctttata 420 gtcctggctg tcatggaact catggtgtag accagggtag actcgaactc agaaatctgc 480 ctccctctgc ctcccgtgtg ctgggattaa aggcttccac caccatgccc agctaaaagt 540 aagcattttc aacaaatggt catggtctgt atgtagagga attcaaattg atccattttt 600 atctccttgt acaaagctga agtctaagca gatcaaggac ctcaacaaaa aattaggaac 660 actaaatcta atagaagaga aagtgggaaa tagcctagaa cattttggca cagggaaaat 720 ttcctgaaca caacagaaat ggctgag 747 // ID SCPV02062786; SV 1; linear; genomic DNA; STD; VRT; 662 BP. XX AC SCPV02062786; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22400.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-662 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-662 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; cce33858f3d24da326490ee695dfdbd6. DR ENA-CON; ML191217. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..662 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22400.1" XX SQ Sequence 662 BP; 88 A; 237 C; 220 G; 117 T; 0 other; agagttcgag cctgcaactt tttttctcct ccgacccagt ccccttagag aggcgatcgg 60 cgtgggggag gggagggtag ggctcgaggc cgcagttcgg tccgctgctt gcgcggcttt 120 ctgttcgcgt cggcagagag gccgcgacaa ccgcatcaga gctgacgtgg gcgcgacgtg 180 cggccgcatc cgccgggttg cctgggcggc ggccgtcggg ctctgcgtcc ccacctcctc 240 ccgtccgtaa tcagtgacga ggtccgctac gtaaaccctt cgcggcgggt aagtggcgcg 300 cctgcctcgc atcacagccc gcggcgggcg gcggctgctc gggacgcgcc tttccgcccg 360 gcctgccctc accgccgccc gcccgcccgc ccgctcgcgg ctcgccaccg agagctcccc 420 gggcacgcag cgggccgcct gcgctcgccc tcttccgtgc ttcgtcttgg taccgacgag 480 aaagcctacc gtcagagagc cctccgggcg gccgctcggg cttctcagtg tgacaggaaa 540 agggcgtcgg tcctgctcgc cacagtcctg ggcccgcagt agcgctttcg tcccagcgtt 600 gcgttcgcag tgtagataga aaataacgtg ccccatttct tccagaaggg tggcgcaggg 660 ac 662 // ID SCPV02062787; SV 1; linear; genomic DNA; STD; VRT; 662 BP. XX AC SCPV02062787; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22400.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-662 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-662 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; cce33858f3d24da326490ee695dfdbd6. DR ENA-CON; ML191217. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..662 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22400.2" XX SQ Sequence 662 BP; 88 A; 237 C; 220 G; 117 T; 0 other; agagttcgag cctgcaactt tttttctcct ccgacccagt ccccttagag aggcgatcgg 60 cgtgggggag gggagggtag ggctcgaggc cgcagttcgg tccgctgctt gcgcggcttt 120 ctgttcgcgt cggcagagag gccgcgacaa ccgcatcaga gctgacgtgg gcgcgacgtg 180 cggccgcatc cgccgggttg cctgggcggc ggccgtcggg ctctgcgtcc ccacctcctc 240 ccgtccgtaa tcagtgacga ggtccgctac gtaaaccctt cgcggcgggt aagtggcgcg 300 cctgcctcgc atcacagccc gcggcgggcg gcggctgctc gggacgcgcc tttccgcccg 360 gcctgccctc accgccgccc gcccgcccgc ccgctcgcgg ctcgccaccg agagctcccc 420 gggcacgcag cgggccgcct gcgctcgccc tcttccgtgc ttcgtcttgg taccgacgag 480 aaagcctacc gtcagagagc cctccgggcg gccgctcggg cttctcagtg tgacaggaaa 540 agggcgtcgg tcctgctcgc cacagtcctg ggcccgcagt agcgctttcg tcccagcgtt 600 gcgttcgcag tgtagataga aaataacgtg ccccatttct tccagaaggg tggcgcaggg 660 ac 662 // ID SCPV02062788; SV 1; linear; genomic DNA; STD; VRT; 1055 BP. XX AC SCPV02062788; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22401.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-1055 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-1055 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 65852c1643cff29da5c4f3d6375c264b. DR ENA-CON; ML191218. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1055 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22401.1" XX SQ Sequence 1055 BP; 254 A; 319 C; 188 G; 294 T; 0 other; tggctaacac tttaattctc cccctccccc cccactgtgg tcattccacc tcttcctttg 60 ggctggcaca gagctctgcc cttggtcccc tcctctcctt attttacacc tttttctttg 120 gtgtatctca accactcatg tggcttcaac tactatctgt acacatctaa cctcacaacc 180 ctaccattct tcagttagtg actcaagagc agcagcagag gaaggagaaa aggggatccc 240 caaacattgg ggtaatttat taaatggaat cataaggacc ttgagagatc atgaagtcca 300 gtcccctgtg ctgaggcagg actaagtaac cttagaccat tcctgacagg tgtttgtcca 360 acctgttttc aaaaacctcc aatcagagat gacccgtacc tgctgaatta aagtgttagc 420 caccccggaa caactcaagt cacaccccac ccccaggcag actgtcccct tgccctcagt 480 ctcccatcct ggaaagtagc agcctcttcc tgcagctccc agctgttgct cctgactctc 540 cctgagggtc acagctcaca gctcataggc tctggcatct gccaggcagg gagggggcct 600 agcttcacca tatgaccaaa cagggctggc aaaccctggc agaaatctgg gcagggagac 660 acatgaccca gtgtgctccc ccattcattg cctctgcctc cagtgatggg gattccacaa 720 cctcccttgg aagcctattc cagcgctaaa ctacccttac agttagaaag tttttcctaa 780 tatctccctt gctgcagatt aaggttatta cttcttgtgc tacctccaat gaacatggag 840 aacaattgtt caccatcctc tttataacag gccttaacat atctgaagac atttatcagg 900 ttcctcctca atttgctttc ctcaagacca aacctgctca gttttttaac atttcctcac 960 aggtcaagtt ttctaaacct tttattattt ctgttgctct cctctcgact ccctccaatt 1020 gtccacatct ttcctaaagt atggtgccca taatt 1055 // ID SCPV02062789; SV 1; linear; genomic DNA; STD; VRT; 1055 BP. XX AC SCPV02062789; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22401.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-1055 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-1055 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 65852c1643cff29da5c4f3d6375c264b. DR ENA-CON; ML191218. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1055 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22401.2" XX SQ Sequence 1055 BP; 254 A; 319 C; 188 G; 294 T; 0 other; tggctaacac tttaattctc cccctccccc cccactgtgg tcattccacc tcttcctttg 60 ggctggcaca gagctctgcc cttggtcccc tcctctcctt attttacacc tttttctttg 120 gtgtatctca accactcatg tggcttcaac tactatctgt acacatctaa cctcacaacc 180 ctaccattct tcagttagtg actcaagagc agcagcagag gaaggagaaa aggggatccc 240 caaacattgg ggtaatttat taaatggaat cataaggacc ttgagagatc atgaagtcca 300 gtcccctgtg ctgaggcagg actaagtaac cttagaccat tcctgacagg tgtttgtcca 360 acctgttttc aaaaacctcc aatcagagat gacccgtacc tgctgaatta aagtgttagc 420 caccccggaa caactcaagt cacaccccac ccccaggcag actgtcccct tgccctcagt 480 ctcccatcct ggaaagtagc agcctcttcc tgcagctccc agctgttgct cctgactctc 540 cctgagggtc acagctcaca gctcataggc tctggcatct gccaggcagg gagggggcct 600 agcttcacca tatgaccaaa cagggctggc aaaccctggc agaaatctgg gcagggagac 660 acatgaccca gtgtgctccc ccattcattg cctctgcctc cagtgatggg gattccacaa 720 cctcccttgg aagcctattc cagcgctaaa ctacccttac agttagaaag tttttcctaa 780 tatctccctt gctgcagatt aaggttatta cttcttgtgc tacctccaat gaacatggag 840 aacaattgtt caccatcctc tttataacag gccttaacat atctgaagac atttatcagg 900 ttcctcctca atttgctttc ctcaagacca aacctgctca gttttttaac atttcctcac 960 aggtcaagtt ttctaaacct tttattattt ctgttgctct cctctcgact ccctccaatt 1020 gtccacatct ttcctaaagt atggtgccca taatt 1055 // ID SCPV02062790; SV 1; linear; genomic DNA; STD; VRT; 784 BP. XX AC SCPV02062790; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22402.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-784 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-784 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 3da27db5702e49a8a81b45ccbb6880bd. DR ENA-CON; ML191219. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..784 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22402.1" XX SQ Sequence 784 BP; 195 A; 196 C; 241 G; 152 T; 0 other; ggagtgggga gtggggccta gccgcagccg gagcctggga gacgatgcat cactgtaagc 60 gataccgttc cccagagcca gacccatacc tgagctaccg ctggaagagg aggcggtctt 120 acagtcggga gcatgaaggt cgactacgat acccatcccg aagggagcct cccccacgga 180 gatcacggtc cagaagccat gatcgtatac cctaccagcg gaggtaccgg gaacaccgtg 240 acagtgatac gtatcggtgt gaagagcgga gcccatcttt tggagaggac tgctatgggt 300 cttcacgttc tcgacatcgg agacggtcac gagagagggc gccgtaccgt acccgcaagc 360 atgcccacca ctgtcacaaa cgccgtacca ggtcttgtag cagtgcttcc tcgagaagcc 420 aacagagcag taagcgcagc agccggagtg tggaagatga caaggagggc cacctggtgt 480 gccggatcgg cgattggctc caagagcgat atgagatcgt ggggaacctg ggtgaaggca 540 cctttggcaa ggtggtggag tgcttggacc atgccagagg gaagtcacag gttgccctga 600 agatcatccg taatgtgggc aagtatcggg aagctgctcg tctagaaatt aatgttctca 660 agaaaatcaa ggagaaagac aaggaaaata agttcctttg tgtcctgatg tctgactggt 720 tcaacttcca tggtcatatg tgcatcgcct ttgagctcct gggcaagaac acctttgagt 780 tcct 784 // ID SCPV02062791; SV 1; linear; genomic DNA; STD; VRT; 784 BP. XX AC SCPV02062791; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22402.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-784 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-784 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 3da27db5702e49a8a81b45ccbb6880bd. DR ENA-CON; ML191219. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..784 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22402.2" XX SQ Sequence 784 BP; 195 A; 196 C; 241 G; 152 T; 0 other; ggagtgggga gtggggccta gccgcagccg gagcctggga gacgatgcat cactgtaagc 60 gataccgttc cccagagcca gacccatacc tgagctaccg ctggaagagg aggcggtctt 120 acagtcggga gcatgaaggt cgactacgat acccatcccg aagggagcct cccccacgga 180 gatcacggtc cagaagccat gatcgtatac cctaccagcg gaggtaccgg gaacaccgtg 240 acagtgatac gtatcggtgt gaagagcgga gcccatcttt tggagaggac tgctatgggt 300 cttcacgttc tcgacatcgg agacggtcac gagagagggc gccgtaccgt acccgcaagc 360 atgcccacca ctgtcacaaa cgccgtacca ggtcttgtag cagtgcttcc tcgagaagcc 420 aacagagcag taagcgcagc agccggagtg tggaagatga caaggagggc cacctggtgt 480 gccggatcgg cgattggctc caagagcgat atgagatcgt ggggaacctg ggtgaaggca 540 cctttggcaa ggtggtggag tgcttggacc atgccagagg gaagtcacag gttgccctga 600 agatcatccg taatgtgggc aagtatcggg aagctgctcg tctagaaatt aatgttctca 660 agaaaatcaa ggagaaagac aaggaaaata agttcctttg tgtcctgatg tctgactggt 720 tcaacttcca tggtcatatg tgcatcgcct ttgagctcct gggcaagaac acctttgagt 780 tcct 784 // ID SCPV02062792; SV 1; linear; genomic DNA; STD; VRT; 976 BP. XX AC SCPV02062792; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22403.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-976 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-976 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 3a4e562dbdf58ec776b9446e4fd59c17. DR ENA-CON; ML191220. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..976 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22403.1" XX SQ Sequence 976 BP; 288 A; 245 C; 208 G; 235 T; 0 other; cactcaccag ctactgagct ctttgctgct tggctgttag gaagcaggta cctgcccacc 60 actgacagat gaggacagat catagcctcc tgtggctacc tcagccaggg cagtgagcag 120 gcagttttta tagaagagat atggccttgc tggacacaat tggtttcagt cttgcacttg 180 acctatgacc ttcttggtca tctataccag ccaagggaca ggaagccatg ggcctaaatg 240 gtttgaaagt ccccacctat aaggatgtct tgtcctataa aaaacaaaca agcaaacaaa 300 acaacaacaa caaaatcccc acacatttac aagtgctgga gatgtagata agctcaattg 360 gtagagagcc tatctagcat gtgcagacat ggttcagttc cctagtactg tgtaacacct 420 ggcatagtag cacatccctg taatcttagc acttggggat agaggtatag agttaagagg 480 atggaaagtc ttcaggtaca tagtgagtta gagatcggca cacatgcatg tgcacacgct 540 catgcacaca cacatgcatg cacatgtaca gaacacacac cagacatgct aaaacaaacc 600 atttaaaaaa caaatcaaag ctcacagcca gtgaaggcaa ggcagaaaca ttcttcagga 660 agcgttggta tttggccact gcttatttta actccagttc ctcttttcat gttgaagcaa 720 aacctgcatt ctttcctgag cttagccaca gtttctgtat aactcagtcc ttatacctaa 780 acctgcttag tgtcagcagc agcaggagca aggctgagac tgcacagcga cacaccaagg 840 gccttcatgg tcccagctct tcttatggga ggtccttgtt gaaacattca ttccaccttc 900 aatctttccc aaggacaatc tgtatctcgt ggtccagaag gctcactgat ggagggaagc 960 acgaccccca ccactc 976 // ID SCPV02062793; SV 1; linear; genomic DNA; STD; VRT; 976 BP. XX AC SCPV02062793; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22403.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-976 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-976 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 3a4e562dbdf58ec776b9446e4fd59c17. DR ENA-CON; ML191220. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..976 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22403.2" XX SQ Sequence 976 BP; 288 A; 245 C; 208 G; 235 T; 0 other; cactcaccag ctactgagct ctttgctgct tggctgttag gaagcaggta cctgcccacc 60 actgacagat gaggacagat catagcctcc tgtggctacc tcagccaggg cagtgagcag 120 gcagttttta tagaagagat atggccttgc tggacacaat tggtttcagt cttgcacttg 180 acctatgacc ttcttggtca tctataccag ccaagggaca ggaagccatg ggcctaaatg 240 gtttgaaagt ccccacctat aaggatgtct tgtcctataa aaaacaaaca agcaaacaaa 300 acaacaacaa caaaatcccc acacatttac aagtgctgga gatgtagata agctcaattg 360 gtagagagcc tatctagcat gtgcagacat ggttcagttc cctagtactg tgtaacacct 420 ggcatagtag cacatccctg taatcttagc acttggggat agaggtatag agttaagagg 480 atggaaagtc ttcaggtaca tagtgagtta gagatcggca cacatgcatg tgcacacgct 540 catgcacaca cacatgcatg cacatgtaca gaacacacac cagacatgct aaaacaaacc 600 atttaaaaaa caaatcaaag ctcacagcca gtgaaggcaa ggcagaaaca ttcttcagga 660 agcgttggta tttggccact gcttatttta actccagttc ctcttttcat gttgaagcaa 720 aacctgcatt ctttcctgag cttagccaca gtttctgtat aactcagtcc ttatacctaa 780 acctgcttag tgtcagcagc agcaggagca aggctgagac tgcacagcga cacaccaagg 840 gccttcatgg tcccagctct tcttatggga ggtccttgtt gaaacattca ttccaccttc 900 aatctttccc aaggacaatc tgtatctcgt ggtccagaag gctcactgat ggagggaagc 960 acgaccccca ccactc 976 // ID SCPV02062794; SV 1; linear; genomic DNA; STD; VRT; 695 BP. XX AC SCPV02062794; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22404.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-695 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-695 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e481b541dfefc43220e5b10956c53a76. DR ENA-CON; ML191221. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..695 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22404.1" XX SQ Sequence 695 BP; 189 A; 172 C; 150 G; 184 T; 0 other; cctttataca atgcacattg ttcactgcct gccttaaccc tttcttaggt ttttccactg 60 gaaattggca gaagtccttc tgtcccagca gaatggctca catacaggca ttctctccta 120 cacctctggc tgtaggggcc tccccttgcc aatttcatac atgacaagct agcacctagt 180 aaaacgaaaa agcatcagca tagggtctaa cgaatgaagc ctgttgtgct ctccaacact 240 gctgctattt cgctgacttc agtgcagtgc cccagtgaga tgtagagcag aatttggccc 300 attttaaggc taaagcatac tgtggattcg aaacagggct gcctggggga gagagaaaaa 360 acctgggtgt tagaaaagtg ttttaggttc tctcaaaggg cactcctttt ggtgagtttc 420 cagtcttttg cggatcatgc aaaatatcaa gaacaaaagc agcatcatga aacttctgca 480 cagaatttcc ctctctgctt tgttctgtga tccatttaaa taagtatggc tgcttaggtg 540 aactcaataa tattggtagc ccccagtaac cacctaagaa tttcttccat attcacaaag 600 tgttctaaca caacagagca gactccagcc acccgtgcag tgggtgtcag caggggcggc 660 tctaggcacc agcaaaacaa gctgctacct agggc 695 // ID SCPV02062795; SV 1; linear; genomic DNA; STD; VRT; 695 BP. XX AC SCPV02062795; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22404.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-695 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-695 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e481b541dfefc43220e5b10956c53a76. DR ENA-CON; ML191221. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..695 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22404.2" XX SQ Sequence 695 BP; 189 A; 172 C; 150 G; 184 T; 0 other; cctttataca atgcacattg ttcactgcct gccttaaccc tttcttaggt ttttccactg 60 gaaattggca gaagtccttc tgtcccagca gaatggctca catacaggca ttctctccta 120 cacctctggc tgtaggggcc tccccttgcc aatttcatac atgacaagct agcacctagt 180 aaaacgaaaa agcatcagca tagggtctaa cgaatgaagc ctgttgtgct ctccaacact 240 gctgctattt cgctgacttc agtgcagtgc cccagtgaga tgtagagcag aatttggccc 300 attttaaggc taaagcatac tgtggattcg aaacagggct gcctggggga gagagaaaaa 360 acctgggtgt tagaaaagtg ttttaggttc tctcaaaggg cactcctttt ggtgagtttc 420 cagtcttttg cggatcatgc aaaatatcaa gaacaaaagc agcatcatga aacttctgca 480 cagaatttcc ctctctgctt tgttctgtga tccatttaaa taagtatggc tgcttaggtg 540 aactcaataa tattggtagc ccccagtaac cacctaagaa tttcttccat attcacaaag 600 tgttctaaca caacagagca gactccagcc acccgtgcag tgggtgtcag caggggcggc 660 tctaggcacc agcaaaacaa gctgctacct agggc 695 // ID SCPV02062796; SV 1; linear; genomic DNA; STD; VRT; 883 BP. XX AC SCPV02062796; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22405.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-883 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-883 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 0ccc916a4fc3a98712b18e274686c078. DR ENA-CON; ML191222. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..883 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22405.1" XX SQ Sequence 883 BP; 224 A; 252 C; 209 G; 198 T; 0 other; gtgcgcctgc gcgatcgctg ggacagggtg tgttcctgct gcgggatcct gttttcggct 60 gatccagttc atcgctctcc acccgagttt cccttgcagg tccctaatca cacccaccag 120 gtgttgctcg ctgcgaacat gtccaagtca ctgaagaagc tggtggagga gagcagggag 180 aagaaccagc cggaagtgga catgagtgac aggggtatct ccagtatgtt ggatgtcaac 240 ggcttgttct ctctagccca catcacacaa ctggtgctca gccacaacaa gctaacaact 300 gtgccaccaa atgtagcgga actgaagaac ttggaggtac taaacttctt caacaatcag 360 atcgaggaac tgcctaccca gatcagcagc ctccagaaac tcaaacacct gaaccttggc 420 atgaataggt tgaacacgct gcctcgagga ttcggctccc tcccggctct ggaggtcctg 480 gacttaactt acaacaacct gaatgaacat tctcttccgg gaaacttctt ctacctcacc 540 accctgcgtg cactctatct aagcgacaac gattttgaaa tcctgcctcc agatattggg 600 aagctcacaa agttgcagat actcagcctc agggataatg acctgatctc actgcctaag 660 gaaatcgggg agctgaccca gctgaaggag ctccacattc aggggaaccg cctgaccgtt 720 ctgcctccag agcttggcaa cttggatcta actggtcaga agcaggtctt caaagcagag 780 aacaacccct gggttacccc gattgctgac cagttccagc ttggcgtctc ccacgttttc 840 gaatatattc gttcagaaac ttacaagtac ctctacggca gac 883 // ID SCPV02062797; SV 1; linear; genomic DNA; STD; VRT; 883 BP. XX AC SCPV02062797; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22405.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-883 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-883 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 0ccc916a4fc3a98712b18e274686c078. DR ENA-CON; ML191222. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..883 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22405.2" XX SQ Sequence 883 BP; 224 A; 252 C; 209 G; 198 T; 0 other; gtgcgcctgc gcgatcgctg ggacagggtg tgttcctgct gcgggatcct gttttcggct 60 gatccagttc atcgctctcc acccgagttt cccttgcagg tccctaatca cacccaccag 120 gtgttgctcg ctgcgaacat gtccaagtca ctgaagaagc tggtggagga gagcagggag 180 aagaaccagc cggaagtgga catgagtgac aggggtatct ccagtatgtt ggatgtcaac 240 ggcttgttct ctctagccca catcacacaa ctggtgctca gccacaacaa gctaacaact 300 gtgccaccaa atgtagcgga actgaagaac ttggaggtac taaacttctt caacaatcag 360 atcgaggaac tgcctaccca gatcagcagc ctccagaaac tcaaacacct gaaccttggc 420 atgaataggt tgaacacgct gcctcgagga ttcggctccc tcccggctct ggaggtcctg 480 gacttaactt acaacaacct gaatgaacat tctcttccgg gaaacttctt ctacctcacc 540 accctgcgtg cactctatct aagcgacaac gattttgaaa tcctgcctcc agatattggg 600 aagctcacaa agttgcagat actcagcctc agggataatg acctgatctc actgcctaag 660 gaaatcgggg agctgaccca gctgaaggag ctccacattc aggggaaccg cctgaccgtt 720 ctgcctccag agcttggcaa cttggatcta actggtcaga agcaggtctt caaagcagag 780 aacaacccct gggttacccc gattgctgac cagttccagc ttggcgtctc ccacgttttc 840 gaatatattc gttcagaaac ttacaagtac ctctacggca gac 883 // ID SCPV02062798; SV 1; linear; genomic DNA; STD; VRT; 648 BP. XX AC SCPV02062798; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22406.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-648 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-648 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d4dbf71fdcb2665ac4062ec11a10170e. DR ENA-CON; ML191223. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..648 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22406.1" XX SQ Sequence 648 BP; 196 A; 141 C; 157 G; 154 T; 0 other; gtatacctga gcctcctgaa ggcgcttctc catgactgtt ggaactgtta aatgaaagag 60 aaactgaagc agtagaatgg gatcaaaata aatgattaga gcatcgattt cgtaagcaag 120 aagctcgcag ggatgtgggt tttgctgttt ttactagtcc catctattct cctgtgctag 180 ttttcagttt ccagaagtac cgcaaactaa aaaaaggaat gatgtgagga tagtgaggcc 240 attgaccaaa tgagttccat tagaagagat cccggcgtga ttacagacac tagggaaatc 300 caaaggcccg actaaacgtt ccaagtgtgt gaccgcccgc aaaattcgtt tccttctttc 360 tctcacaaac aaacaatgac ctggaagaag atggagtagg cgggaacaag tgaaacaagt 420 caaaaaggct tcgctccttt ccaatctctt tatttgtcct tatccaacaa acatttgttg 480 atggttctgc tgttaattga ggaaccgaag tgagtgaggc ctgagtctcc agtccacaag 540 tgaggcacaa gaggccggaa gcattgtgcc caacaaccgc accacagcgg aggcccaagc 600 cggaagactc ctccaggaat tcggtttaag ttgcaggagc acagagag 648 // ID SCPV02062799; SV 1; linear; genomic DNA; STD; VRT; 648 BP. XX AC SCPV02062799; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22406.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-648 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-648 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d4dbf71fdcb2665ac4062ec11a10170e. DR ENA-CON; ML191223. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..648 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22406.2" XX SQ Sequence 648 BP; 196 A; 141 C; 157 G; 154 T; 0 other; gtatacctga gcctcctgaa ggcgcttctc catgactgtt ggaactgtta aatgaaagag 60 aaactgaagc agtagaatgg gatcaaaata aatgattaga gcatcgattt cgtaagcaag 120 aagctcgcag ggatgtgggt tttgctgttt ttactagtcc catctattct cctgtgctag 180 ttttcagttt ccagaagtac cgcaaactaa aaaaaggaat gatgtgagga tagtgaggcc 240 attgaccaaa tgagttccat tagaagagat cccggcgtga ttacagacac tagggaaatc 300 caaaggcccg actaaacgtt ccaagtgtgt gaccgcccgc aaaattcgtt tccttctttc 360 tctcacaaac aaacaatgac ctggaagaag atggagtagg cgggaacaag tgaaacaagt 420 caaaaaggct tcgctccttt ccaatctctt tatttgtcct tatccaacaa acatttgttg 480 atggttctgc tgttaattga ggaaccgaag tgagtgaggc ctgagtctcc agtccacaag 540 tgaggcacaa gaggccggaa gcattgtgcc caacaaccgc accacagcgg aggcccaagc 600 cggaagactc ctccaggaat tcggtttaag ttgcaggagc acagagag 648 // ID SCPV02062800; SV 1; linear; genomic DNA; STD; VRT; 881 BP. XX AC SCPV02062800; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22407.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-881 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-881 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 583dcbb24cea83ed11a0a908c9829a71. DR ENA-CON; ML191224. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..881 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22407.1" XX SQ Sequence 881 BP; 211 A; 236 C; 260 G; 174 T; 0 other; agagagcaag agagaggaac tggggtttgc gaaccttgtg gctttgtctg tttcctgttt 60 cagcaaggct gctgtgcaaa aagaaagacg gtaccaagaa gggacgtgtt aatggggccc 120 agacctatgg attgaaagcg tgtgctttac ccatctgctg tcttgctcca tctgagacca 180 gagccaagat ctgcccagga ctggaatgct ttcccgagtg gcttgagttg gagcctggga 240 ctaggagcag ccttattgag gtacaattca tgtgcttgtg ggtttggatg gcacaatctg 300 tgtctgggct aaggaaagca gacttggcac caacattaac cctgacagat ccaggcatct 360 attccaggaa ctggaagcca agcgcaacag gtgcttggag gtcatcatga tcagcccaga 420 ccccaggccc tcccctggct tggcccggtg ggctgagagc tatgaggcca agtgtgagcg 480 caggcaagag atccgtgaaa gccgccgctg ccgtcccaat gtgaccactt gccgccaggt 540 ggggaagacg ctgaggatcc aacagagaga gcagctccag agagctcgac tgcagcagtt 600 cttcaggagg aggaacctgg agctagagga gaagggcaaa gcgcagcatc cccaggccag 660 ggagcaaggg ccctccaggc ggccaggaca ggtgactgtc ctcaaggaac ccttgtcttg 720 tgccagaagg atttcttctc ccagagagca ggtgacaggc accagctctg aagtctttcc 780 agcccagcat cctcctccct caggcatctg cagggatctg tctgaccacc tctcctcaca 840 ggctgggggc cttcctccac aggacactcc catataagaa a 881 // ID SCPV02062801; SV 1; linear; genomic DNA; STD; VRT; 881 BP. XX AC SCPV02062801; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22407.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-881 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-881 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 583dcbb24cea83ed11a0a908c9829a71. DR ENA-CON; ML191224. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..881 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22407.2" XX SQ Sequence 881 BP; 211 A; 236 C; 260 G; 174 T; 0 other; agagagcaag agagaggaac tggggtttgc gaaccttgtg gctttgtctg tttcctgttt 60 cagcaaggct gctgtgcaaa aagaaagacg gtaccaagaa gggacgtgtt aatggggccc 120 agacctatgg attgaaagcg tgtgctttac ccatctgctg tcttgctcca tctgagacca 180 gagccaagat ctgcccagga ctggaatgct ttcccgagtg gcttgagttg gagcctggga 240 ctaggagcag ccttattgag gtacaattca tgtgcttgtg ggtttggatg gcacaatctg 300 tgtctgggct aaggaaagca gacttggcac caacattaac cctgacagat ccaggcatct 360 attccaggaa ctggaagcca agcgcaacag gtgcttggag gtcatcatga tcagcccaga 420 ccccaggccc tcccctggct tggcccggtg ggctgagagc tatgaggcca agtgtgagcg 480 caggcaagag atccgtgaaa gccgccgctg ccgtcccaat gtgaccactt gccgccaggt 540 ggggaagacg ctgaggatcc aacagagaga gcagctccag agagctcgac tgcagcagtt 600 cttcaggagg aggaacctgg agctagagga gaagggcaaa gcgcagcatc cccaggccag 660 ggagcaaggg ccctccaggc ggccaggaca ggtgactgtc ctcaaggaac ccttgtcttg 720 tgccagaagg atttcttctc ccagagagca ggtgacaggc accagctctg aagtctttcc 780 agcccagcat cctcctccct caggcatctg cagggatctg tctgaccacc tctcctcaca 840 ggctgggggc cttcctccac aggacactcc catataagaa a 881 // ID SCPV02062802; SV 1; linear; genomic DNA; STD; VRT; 782 BP. XX AC SCPV02062802; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22408.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-782 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-782 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 779f23557ea555ef402f48052c43962d. DR ENA-CON; ML191225. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..782 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22408.1" XX SQ Sequence 782 BP; 112 A; 286 C; 280 G; 104 T; 0 other; atggggggtg gtgcagcagc cattttgtca gtcgccagcg gatcccgcgc gctgccgaag 60 tgcagttccc cctggttacc aggtggtcgg ttcttcccct ccgcgctgcc ggcgggcggg 120 caggcgggcg ccctcggaga gagagagcgc ccaccacagg cgtcgccttc gctcctcccc 180 gggtcgccgc ggcccctctc ccgagccgcg gcgctgtctg cctctcctta ccactgacag 240 gcgcccccga gggcgctgcc ggcggctatg tcaagcgagg agagctaccg ggccatcctg 300 cgctacctga cgaacgaacg cgagccctat gcgccgggca ccgagggcaa tgtcaagcgg 360 aagatccgca aggccgccgc ctgctacgtc gtgcgcggcg ggaccctcta ctaccagcgg 420 cggcagcggc accgcaagac cttcgccgag ctggaggtgg tgttgcagcc ggagcgtcgc 480 cagggcctca tcgaggcggc ccatttgggc cccgggggca cccaccacac ccggcaccag 540 acctggcacg acctgtccaa gacctactgg tggcgaggtg agtgcgccgg gccccggggg 600 ccgagggggg cggctcggcc cggcctgcgt gcgcacccgc gagccctcaa gtctggggac 660 cgggccgtat ccttagcccg gaggcgtgcg tgcgtccgtt cggctgcgcg gcaaccgcgg 720 gacccagccg agccaagcgg agcagagcgg gctggggacg cgaaagcagc cgcacttccc 780 ct 782 // ID SCPV02062803; SV 1; linear; genomic DNA; STD; VRT; 782 BP. XX AC SCPV02062803; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22408.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-782 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-782 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 779f23557ea555ef402f48052c43962d. DR ENA-CON; ML191225. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..782 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22408.2" XX SQ Sequence 782 BP; 112 A; 286 C; 280 G; 104 T; 0 other; atggggggtg gtgcagcagc cattttgtca gtcgccagcg gatcccgcgc gctgccgaag 60 tgcagttccc cctggttacc aggtggtcgg ttcttcccct ccgcgctgcc ggcgggcggg 120 caggcgggcg ccctcggaga gagagagcgc ccaccacagg cgtcgccttc gctcctcccc 180 gggtcgccgc ggcccctctc ccgagccgcg gcgctgtctg cctctcctta ccactgacag 240 gcgcccccga gggcgctgcc ggcggctatg tcaagcgagg agagctaccg ggccatcctg 300 cgctacctga cgaacgaacg cgagccctat gcgccgggca ccgagggcaa tgtcaagcgg 360 aagatccgca aggccgccgc ctgctacgtc gtgcgcggcg ggaccctcta ctaccagcgg 420 cggcagcggc accgcaagac cttcgccgag ctggaggtgg tgttgcagcc ggagcgtcgc 480 cagggcctca tcgaggcggc ccatttgggc cccgggggca cccaccacac ccggcaccag 540 acctggcacg acctgtccaa gacctactgg tggcgaggtg agtgcgccgg gccccggggg 600 ccgagggggg cggctcggcc cggcctgcgt gcgcacccgc gagccctcaa gtctggggac 660 cgggccgtat ccttagcccg gaggcgtgcg tgcgtccgtt cggctgcgcg gcaaccgcgg 720 gacccagccg agccaagcgg agcagagcgg gctggggacg cgaaagcagc cgcacttccc 780 ct 782 // ID SCPV02062804; SV 1; linear; genomic DNA; STD; VRT; 809 BP. XX AC SCPV02062804; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22409.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-809 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-809 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 5ca916c5f8d5d99973bc828b8e420158. DR ENA-CON; ML191226. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..809 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22409.1" XX SQ Sequence 809 BP; 207 A; 221 C; 211 G; 170 T; 0 other; atgctcaaag ttcttttaag ttgaaggtgg aaccccccag aggtataaag cccaagactt 60 gggagccagc caggagacag agcagggcag gcaggtctga gaagataccg gctccagggg 120 cagactccat aacccccagg aatctctgaa ttaaactgag tcattttgca ccagaaggca 180 ctagaatcac ccagagtcca gcgaatctcc acttccaaag cagcaactgg cactggccac 240 agccttgacc agaaaatggc ctgggcaact ccagaggata cagaaagact caggcagccc 300 cgggcaatcc atggagcaat ggactggatc tgcgggttca gaaatcaatc tgatgagggg 360 ttaccaactg tcactccttg cagtaggctg gggtcttccc aactctgctt ctcagggggc 420 acctgccatg tccgcccatc caccttacct gggatgcaga accccagttt cagcagggca 480 gcacagaccg tgtttgatcg ctgtgaaatg agcagatttt tcctggtcct gtcagtgttc 540 tcacagaaaa gcagagctaa cacattcagg gacatgtgga ggaatagcct agtgacattg 600 ccggtgagta gccacagatg gccatcttgg taccacctca gcctgtgcag gagtcgggac 660 agcagctggt cttctggtct ggtcttgttc ctagacctgg gcaggtctga gttccaggtc 720 taccacattt agacctggct gaggtccaga atcatccctt agccaggttt ccctgctata 780 ccctaccctg aagtactaac aacaggcag 809 // ID SCPV02062805; SV 1; linear; genomic DNA; STD; VRT; 809 BP. XX AC SCPV02062805; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22409.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-809 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-809 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 5ca916c5f8d5d99973bc828b8e420158. DR ENA-CON; ML191226. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..809 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22409.2" XX SQ Sequence 809 BP; 207 A; 221 C; 211 G; 170 T; 0 other; atgctcaaag ttcttttaag ttgaaggtgg aaccccccag aggtataaag cccaagactt 60 gggagccagc caggagacag agcagggcag gcaggtctga gaagataccg gctccagggg 120 cagactccat aacccccagg aatctctgaa ttaaactgag tcattttgca ccagaaggca 180 ctagaatcac ccagagtcca gcgaatctcc acttccaaag cagcaactgg cactggccac 240 agccttgacc agaaaatggc ctgggcaact ccagaggata cagaaagact caggcagccc 300 cgggcaatcc atggagcaat ggactggatc tgcgggttca gaaatcaatc tgatgagggg 360 ttaccaactg tcactccttg cagtaggctg gggtcttccc aactctgctt ctcagggggc 420 acctgccatg tccgcccatc caccttacct gggatgcaga accccagttt cagcagggca 480 gcacagaccg tgtttgatcg ctgtgaaatg agcagatttt tcctggtcct gtcagtgttc 540 tcacagaaaa gcagagctaa cacattcagg gacatgtgga ggaatagcct agtgacattg 600 ccggtgagta gccacagatg gccatcttgg taccacctca gcctgtgcag gagtcgggac 660 agcagctggt cttctggtct ggtcttgttc ctagacctgg gcaggtctga gttccaggtc 720 taccacattt agacctggct gaggtccaga atcatccctt agccaggttt ccctgctata 780 ccctaccctg aagtactaac aacaggcag 809 // ID SCPV02062806; SV 1; linear; genomic DNA; STD; VRT; 631 BP. XX AC SCPV02062806; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22410.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-631 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-631 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 962c8315429d505901f29dc42bb9dc09. DR ENA-CON; ML191227. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..631 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22410.1" XX SQ Sequence 631 BP; 156 A; 149 C; 185 G; 141 T; 0 other; gcacttcagt tgaggttggg aatccgggcg gagacctgct cttggctccc agggcatgga 60 tcgcgtgtgg cgtgcgtggg acgggcagtc cttcaaagag aaccagcctg agtcgccgag 120 tgccaggggt atcgtcgtct cttggctcag cagggctgag tgggagcaag tgacggttta 180 tctgttctgc gatgatcaca agttgcagca gtatgcgctg aaccgaatta cagtatggag 240 gagcaggtta ggcaacgaac tgcccctggc agtggcttct actgctgacc tggtaagatg 300 caagctcatc gatgcagccg gtaccttggg cactgatgaa cttagactgc tctatggcat 360 gacatgggtc aggtttgtga atcttatctc agagaggaag acaaaatgtt ctaaccttcc 420 cctcaaatac ctggctcagg aggtgaacat tccagactgg attgttgaac ttcgccacaa 480 cttgacccac aagaaaatgc cccatataaa tgagtgccgc agaggttgct actttgtcct 540 caactggctc cagaagacat actggagccg tcaactggaa ggcagcctga aagaaacctg 600 ggagttggat gaggaccagc tagatgcaga a 631 // ID SCPV02062807; SV 1; linear; genomic DNA; STD; VRT; 631 BP. XX AC SCPV02062807; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22410.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-631 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-631 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 962c8315429d505901f29dc42bb9dc09. DR ENA-CON; ML191227. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..631 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22410.2" XX SQ Sequence 631 BP; 156 A; 149 C; 185 G; 141 T; 0 other; gcacttcagt tgaggttggg aatccgggcg gagacctgct cttggctccc agggcatgga 60 tcgcgtgtgg cgtgcgtggg acgggcagtc cttcaaagag aaccagcctg agtcgccgag 120 tgccaggggt atcgtcgtct cttggctcag cagggctgag tgggagcaag tgacggttta 180 tctgttctgc gatgatcaca agttgcagca gtatgcgctg aaccgaatta cagtatggag 240 gagcaggtta ggcaacgaac tgcccctggc agtggcttct actgctgacc tggtaagatg 300 caagctcatc gatgcagccg gtaccttggg cactgatgaa cttagactgc tctatggcat 360 gacatgggtc aggtttgtga atcttatctc agagaggaag acaaaatgtt ctaaccttcc 420 cctcaaatac ctggctcagg aggtgaacat tccagactgg attgttgaac ttcgccacaa 480 cttgacccac aagaaaatgc cccatataaa tgagtgccgc agaggttgct actttgtcct 540 caactggctc cagaagacat actggagccg tcaactggaa ggcagcctga aagaaacctg 600 ggagttggat gaggaccagc tagatgcaga a 631 // ID SCPV02062808; SV 1; linear; genomic DNA; STD; VRT; 681 BP. XX AC SCPV02062808; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22411.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-681 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-681 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; cef2d1dcd9a69dc766c4648caeb3ff37. DR ENA-CON; ML191228. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..681 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22411.1" XX SQ Sequence 681 BP; 83 A; 250 C; 257 G; 91 T; 0 other; gagccgggga gcaggcgggc tgcccacgcc gttctgacca gacggagccg acctgattgt 60 ctccgatgca gactccacca tcattgccgc tcaatcggga cctgtccggc ccggccgctc 120 agtgcgggct ccggcccgtc agcccgcacg gggcatgggg ctcgctcggg tcgcagcccg 180 ggcccgccgc cactcgccgg ggctgcccct gcgctccgaa gcgatctttc tagcgccgat 240 cgccgcgcgc cttagcccct tgggggcatc gcactttgga ggatggcttg gctctctcca 300 gcttccgatc gtccttcccc gggctccggc gacgctcctg agctcgcacc ggctccgggg 360 ctccccgacc acgacaactc aagaaacagc ccagccctgg ttccggcccg ggctcctccc 420 cgccgcgccg cggccgcctg cgccgcgccc tgatcgcgtg ttggggggct ccgtcccggc 480 agctggtggc actgtccggt ccgtaggggc gaccggctcc ggactccgag gggctgcgac 540 tgagccccgg ctgcgagtgg ctgcgagcga tcggcgcgcg ggcggggcac accgggcggg 600 gggaccggag gggcggcggg atggagggag cgcgccgagc gacagggagg agggagcgaa 660 cgagcgagcg gcggagggag g 681 // ID SCPV02062809; SV 1; linear; genomic DNA; STD; VRT; 681 BP. XX AC SCPV02062809; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22411.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-681 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-681 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; cef2d1dcd9a69dc766c4648caeb3ff37. DR ENA-CON; ML191228. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..681 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22411.2" XX SQ Sequence 681 BP; 83 A; 250 C; 257 G; 91 T; 0 other; gagccgggga gcaggcgggc tgcccacgcc gttctgacca gacggagccg acctgattgt 60 ctccgatgca gactccacca tcattgccgc tcaatcggga cctgtccggc ccggccgctc 120 agtgcgggct ccggcccgtc agcccgcacg gggcatgggg ctcgctcggg tcgcagcccg 180 ggcccgccgc cactcgccgg ggctgcccct gcgctccgaa gcgatctttc tagcgccgat 240 cgccgcgcgc cttagcccct tgggggcatc gcactttgga ggatggcttg gctctctcca 300 gcttccgatc gtccttcccc gggctccggc gacgctcctg agctcgcacc ggctccgggg 360 ctccccgacc acgacaactc aagaaacagc ccagccctgg ttccggcccg ggctcctccc 420 cgccgcgccg cggccgcctg cgccgcgccc tgatcgcgtg ttggggggct ccgtcccggc 480 agctggtggc actgtccggt ccgtaggggc gaccggctcc ggactccgag gggctgcgac 540 tgagccccgg ctgcgagtgg ctgcgagcga tcggcgcgcg ggcggggcac accgggcggg 600 gggaccggag gggcggcggg atggagggag cgcgccgagc gacagggagg agggagcgaa 660 cgagcgagcg gcggagggag g 681 // ID SCPV02062810; SV 1; linear; genomic DNA; STD; VRT; 617 BP. XX AC SCPV02062810; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22412.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-617 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-617 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 56d0d889ca853ae289a4be8d5aeb60d1. DR ENA-CON; ML191229. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..617 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22412.1" XX SQ Sequence 617 BP; 200 A; 102 C; 133 G; 182 T; 0 other; tgggagagct ggaatgacat ttgagttgtt acttgcgaat tctcccaact gtgcccatca 60 attaaactga atcatgagca gaggaaatcc ttgctgagaa agaaatagaa aaagagacag 120 acagacgtgc tgcctgagag cgggtaaagg cttgttgcta agagaagatc caaagagaca 180 gtttttgagg gggacctggg gcctttttat tatctttttt tttcttggat aaagaatatt 240 aatggctgga actaataaga gaaaagaccg aagagctggg cacagcaaat gatctacaga 300 ctgctggcta aaaccacaga gctaaccatt tgttgacaac attcaaggta ttttcacatt 360 ccttttattt acattatgtc actataaatg cttgtgcata tatctttgtg catatagcta 420 tttttgtaag gaaaaaaaaa atcctaaaat gaccaacaaa gaatgactaa caaagaatga 480 ctggtccaaa agtgtgtgta ttttgaggat tcttaatgta tattgacatt ttttctcttt 540 ctttttccat ctggaccgtt tattaccttt tgtcaaaggg gaggcacaat agcaactggg 600 gctcaacagg agactgg 617 // ID SCPV02062811; SV 1; linear; genomic DNA; STD; VRT; 617 BP. XX AC SCPV02062811; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22412.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-617 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-617 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 56d0d889ca853ae289a4be8d5aeb60d1. DR ENA-CON; ML191229. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..617 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22412.2" XX SQ Sequence 617 BP; 200 A; 102 C; 133 G; 182 T; 0 other; tgggagagct ggaatgacat ttgagttgtt acttgcgaat tctcccaact gtgcccatca 60 attaaactga atcatgagca gaggaaatcc ttgctgagaa agaaatagaa aaagagacag 120 acagacgtgc tgcctgagag cgggtaaagg cttgttgcta agagaagatc caaagagaca 180 gtttttgagg gggacctggg gcctttttat tatctttttt tttcttggat aaagaatatt 240 aatggctgga actaataaga gaaaagaccg aagagctggg cacagcaaat gatctacaga 300 ctgctggcta aaaccacaga gctaaccatt tgttgacaac attcaaggta ttttcacatt 360 ccttttattt acattatgtc actataaatg cttgtgcata tatctttgtg catatagcta 420 tttttgtaag gaaaaaaaaa atcctaaaat gaccaacaaa gaatgactaa caaagaatga 480 ctggtccaaa agtgtgtgta ttttgaggat tcttaatgta tattgacatt ttttctcttt 540 ctttttccat ctggaccgtt tattaccttt tgtcaaaggg gaggcacaat agcaactggg 600 gctcaacagg agactgg 617 // ID SCPV02062812; SV 1; linear; genomic DNA; STD; VRT; 872 BP. XX AC SCPV02062812; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22413.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-872 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-872 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; cb25d45e244d68a52e700fd79ec20bb5. DR ENA-CON; ML191230. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..872 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22413.1" XX SQ Sequence 872 BP; 333 A; 190 C; 198 G; 151 T; 0 other; tgagaaatcg gctgcacttc ccatcttttc ctcgtttgtc agcaattggg atgaagccac 60 caaaagatct ttgcttaata agaagaaaaa agaggcaagg agaaaacgaa gagaaagaaa 120 ttttgaaaaa caaaaggaga ggaagaagaa gaggcagcag gctaggaaga ctgcatcagt 180 tcttagtaaa gatgatgtgg cacctgaaag tggtgatact acagtgaaga aacctgaatc 240 aaagaaggaa cagaccccag agcatgggaa gaaaaaacgt ggcagaggaa aagcccaagt 300 taaagcaaca aatgaatccg aagacgaaat cccacagctg gtaccaatag gaaagaagac 360 tccagctaat gaaaaagtag agattcaaaa acatgccaca ggaaagaagt ctccagcaaa 420 gagtcctaat cccagcacac ctcgtgggaa gaaaagaaag gctttgccag catctgagac 480 cccaaaagct gcagagtctg agaccccagg gaaaagccca tataagaaac caaaaatcaa 540 agaagaggca gtgaaggaaa aaagtccttc gctggggaaa aaagatgcga gacagactcc 600 aaaaaagcca gaggccaagt ttttcaccac tcctagtaaa tctgtgagaa aagcttccca 660 cacccccaaa aaatggccca aaaaacccaa agtaccccag tcgacctaaa gtcagtgatt 720 caactggaag gaaacctcaa tgctgcctcc agagcttttt ggaaatactc agatcctggc 780 cgcctttgta accttctcta aacgtcaggc ctggacttaa aagatttttt aaaacctcca 840 taagtagtcc aggggcggtg gctcacgcct gt 872 // ID SCPV02062813; SV 1; linear; genomic DNA; STD; VRT; 872 BP. XX AC SCPV02062813; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22413.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-872 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-872 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; cb25d45e244d68a52e700fd79ec20bb5. DR ENA-CON; ML191230. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..872 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22413.2" XX SQ Sequence 872 BP; 333 A; 190 C; 198 G; 151 T; 0 other; tgagaaatcg gctgcacttc ccatcttttc ctcgtttgtc agcaattggg atgaagccac 60 caaaagatct ttgcttaata agaagaaaaa agaggcaagg agaaaacgaa gagaaagaaa 120 ttttgaaaaa caaaaggaga ggaagaagaa gaggcagcag gctaggaaga ctgcatcagt 180 tcttagtaaa gatgatgtgg cacctgaaag tggtgatact acagtgaaga aacctgaatc 240 aaagaaggaa cagaccccag agcatgggaa gaaaaaacgt ggcagaggaa aagcccaagt 300 taaagcaaca aatgaatccg aagacgaaat cccacagctg gtaccaatag gaaagaagac 360 tccagctaat gaaaaagtag agattcaaaa acatgccaca ggaaagaagt ctccagcaaa 420 gagtcctaat cccagcacac ctcgtgggaa gaaaagaaag gctttgccag catctgagac 480 cccaaaagct gcagagtctg agaccccagg gaaaagccca tataagaaac caaaaatcaa 540 agaagaggca gtgaaggaaa aaagtccttc gctggggaaa aaagatgcga gacagactcc 600 aaaaaagcca gaggccaagt ttttcaccac tcctagtaaa tctgtgagaa aagcttccca 660 cacccccaaa aaatggccca aaaaacccaa agtaccccag tcgacctaaa gtcagtgatt 720 caactggaag gaaacctcaa tgctgcctcc agagcttttt ggaaatactc agatcctggc 780 cgcctttgta accttctcta aacgtcaggc ctggacttaa aagatttttt aaaacctcca 840 taagtagtcc aggggcggtg gctcacgcct gt 872 // ID SCPV02062814; SV 1; linear; genomic DNA; STD; VRT; 707 BP. XX AC SCPV02062814; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22414.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-707 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-707 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; ac79149b99922a144fc7072a82a334ca. DR ENA-CON; ML191231. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..707 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22414.1" XX SQ Sequence 707 BP; 233 A; 188 C; 152 G; 134 T; 0 other; caacggtgac atcattatgg aagagttgag tgaacaaaac acttcagaaa ccaactcata 60 ctcactgagt tcttcagatg ccaaaggtaa tcaccaagat ggcctccata ccctaccatc 120 caccaaagag tctgccagta cccaggtaat cacaccccga gccccttgtc cagatcggaa 180 ctcacttagt caggtagaag atatggaaac tgaaagtcca gaggttaaga gagttatccc 240 gtctcctgtt agaactggag agggctctaa ccttagcaag ggcttcatgg atgacaatgg 300 actgccttcc agcaaagacg agaacgtcaa tggagaatcc caaagaaaga cggtcatcac 360 agaagtcacc acaatgacat ctactgttgc cacagaatca aaaactgtca ttaaggtagc 420 aaaaggagac aagcaaactg tggtttcttc cacagaaaat tgtgccagat ccacagtcac 480 aactaccact accactgtga caaagctctc cacaccctcc ccagacaccg gtgtggatac 540 catctctgta aaggagcaga gtaaaaccgt ggtcaccacg acagtgacgg actccttgac 600 cactgcagga agcacactgg tgacatctat gaccgtgagc aaagagtact ccacgagaga 660 cagggtaaag ctgatgaagt tctcacgacc caagaagacc cgctcag 707 // ID SCPV02062815; SV 1; linear; genomic DNA; STD; VRT; 707 BP. XX AC SCPV02062815; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22414.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-707 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-707 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; ac79149b99922a144fc7072a82a334ca. DR ENA-CON; ML191231. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..707 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22414.2" XX SQ Sequence 707 BP; 233 A; 188 C; 152 G; 134 T; 0 other; caacggtgac atcattatgg aagagttgag tgaacaaaac acttcagaaa ccaactcata 60 ctcactgagt tcttcagatg ccaaaggtaa tcaccaagat ggcctccata ccctaccatc 120 caccaaagag tctgccagta cccaggtaat cacaccccga gccccttgtc cagatcggaa 180 ctcacttagt caggtagaag atatggaaac tgaaagtcca gaggttaaga gagttatccc 240 gtctcctgtt agaactggag agggctctaa ccttagcaag ggcttcatgg atgacaatgg 300 actgccttcc agcaaagacg agaacgtcaa tggagaatcc caaagaaaga cggtcatcac 360 agaagtcacc acaatgacat ctactgttgc cacagaatca aaaactgtca ttaaggtagc 420 aaaaggagac aagcaaactg tggtttcttc cacagaaaat tgtgccagat ccacagtcac 480 aactaccact accactgtga caaagctctc cacaccctcc ccagacaccg gtgtggatac 540 catctctgta aaggagcaga gtaaaaccgt ggtcaccacg acagtgacgg actccttgac 600 cactgcagga agcacactgg tgacatctat gaccgtgagc aaagagtact ccacgagaga 660 cagggtaaag ctgatgaagt tctcacgacc caagaagacc cgctcag 707 // ID SCPV02062816; SV 1; linear; genomic DNA; STD; VRT; 629 BP. XX AC SCPV02062816; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22415.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-629 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-629 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 21a17b2ceacda9964c010033ba77b2ad. DR ENA-CON; ML191232. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..629 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22415.1" XX SQ Sequence 629 BP; 154 A; 184 C; 201 G; 90 T; 0 other; gtgccaacca cgtgctgaag ggaaaggaca cgagttgcct agcagagcgg tggtctggca 60 agaaggggga catagcctcc ctgggaggga agaaatccaa gaagaagcgc agcggagtca 120 agggctcctc cagtggcagc agcaagcaga ggaaggaact ggggggcctg ggggaggatg 180 ccaacgccga ggaggaggag ggtgtgcaga aggcatcgcc cctcccacac agcccccctg 240 atgagctgtg agcccagctt agtgtccttg aatcaagacc cctgacttca gagcttggga 300 cacgcacagc gcagcgcagc gcagctccag caaggacagc tgctgtccag catcaggtct 360 cctcccttgg ctgtgcccct ttccttccct tgaacaacag caagaggtgg aaggatctgg 420 ggtgctggga gacggcaccc caaagggaag aggaggagga gcagaaggca gctctctttc 480 tacacagtcc ccctcacgag ctccggggtc cacccagcat ccccaggctg agatccaggc 540 tcctgacatg gaagctgaag agcatgaggc acataagatg ctcaccagcg cccccttcag 600 ccaggaagga ctccgtgcag cctcagcag 629 // ID SCPV02062817; SV 1; linear; genomic DNA; STD; VRT; 629 BP. XX AC SCPV02062817; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22415.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-629 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-629 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 21a17b2ceacda9964c010033ba77b2ad. DR ENA-CON; ML191232. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..629 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22415.2" XX SQ Sequence 629 BP; 154 A; 184 C; 201 G; 90 T; 0 other; gtgccaacca cgtgctgaag ggaaaggaca cgagttgcct agcagagcgg tggtctggca 60 agaaggggga catagcctcc ctgggaggga agaaatccaa gaagaagcgc agcggagtca 120 agggctcctc cagtggcagc agcaagcaga ggaaggaact ggggggcctg ggggaggatg 180 ccaacgccga ggaggaggag ggtgtgcaga aggcatcgcc cctcccacac agcccccctg 240 atgagctgtg agcccagctt agtgtccttg aatcaagacc cctgacttca gagcttggga 300 cacgcacagc gcagcgcagc gcagctccag caaggacagc tgctgtccag catcaggtct 360 cctcccttgg ctgtgcccct ttccttccct tgaacaacag caagaggtgg aaggatctgg 420 ggtgctggga gacggcaccc caaagggaag aggaggagga gcagaaggca gctctctttc 480 tacacagtcc ccctcacgag ctccggggtc cacccagcat ccccaggctg agatccaggc 540 tcctgacatg gaagctgaag agcatgaggc acataagatg ctcaccagcg cccccttcag 600 ccaggaagga ctccgtgcag cctcagcag 629 // ID SCPV02062818; SV 1; linear; genomic DNA; STD; VRT; 785 BP. XX AC SCPV02062818; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22416.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-785 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-785 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 1b50a089866dd16832ac8ff4f3931fbb. DR ENA-CON; ML191233. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..785 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22416.1" XX SQ Sequence 785 BP; 183 A; 232 C; 238 G; 132 T; 0 other; ggccacagcg ccgggggagg ctgcgatgtg gctgctgctc tgggccccgc gaaggtgctg 60 gggacgaaag aagccgagct gccaggggca ctgaggcaga tgtggcgctc ccgctcctgg 120 gacgtgccac agatcccagc tgaggttccc cagacacaga aagccatcac caagtcgggc 180 ctccagcacc tggcaccccc tcctcccacg cccggggccc cgtgcggtga atctgaggag 240 cagatccaga gcactgtgga ctggagtgag tcagcagtgt atggggagca catctggttt 300 gagaccaacg tgtccggtga cttctgctat gtcggggagc agcactgtgt agctaagatg 360 ctgccgaagt cagcgcccag aaaaaagtgt gcagcctgta agatcgtggt gcatacccaa 420 tgcattaagc agctggaaaa gatcaatttc cgctgtaagc cgtccttccg cgaatcaggc 480 tccaggaatg tccgtgagcc aaccttcgta agacaccact gggtccacag acgacgccag 540 gatggcaagt gtcggcactg tgggaagggc ttccagcaga agttcacctt ccacagcaag 600 gagatcgtag ccatcagctg ctcctggtgc aaacaggcat accacagcaa ggtgtcgtgc 660 ttcatgatgc aacagattga ggaaccctgc tccctagggg tgcatgcagc cgtggtcatc 720 ccacccacct ggatcctgcg ggcccggagg ccccagaaca ccctcaaggc cagcaagaag 780 aaaaa 785 // ID SCPV02062819; SV 1; linear; genomic DNA; STD; VRT; 785 BP. XX AC SCPV02062819; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22416.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-785 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-785 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 1b50a089866dd16832ac8ff4f3931fbb. DR ENA-CON; ML191233. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..785 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22416.2" XX SQ Sequence 785 BP; 183 A; 232 C; 238 G; 132 T; 0 other; ggccacagcg ccgggggagg ctgcgatgtg gctgctgctc tgggccccgc gaaggtgctg 60 gggacgaaag aagccgagct gccaggggca ctgaggcaga tgtggcgctc ccgctcctgg 120 gacgtgccac agatcccagc tgaggttccc cagacacaga aagccatcac caagtcgggc 180 ctccagcacc tggcaccccc tcctcccacg cccggggccc cgtgcggtga atctgaggag 240 cagatccaga gcactgtgga ctggagtgag tcagcagtgt atggggagca catctggttt 300 gagaccaacg tgtccggtga cttctgctat gtcggggagc agcactgtgt agctaagatg 360 ctgccgaagt cagcgcccag aaaaaagtgt gcagcctgta agatcgtggt gcatacccaa 420 tgcattaagc agctggaaaa gatcaatttc cgctgtaagc cgtccttccg cgaatcaggc 480 tccaggaatg tccgtgagcc aaccttcgta agacaccact gggtccacag acgacgccag 540 gatggcaagt gtcggcactg tgggaagggc ttccagcaga agttcacctt ccacagcaag 600 gagatcgtag ccatcagctg ctcctggtgc aaacaggcat accacagcaa ggtgtcgtgc 660 ttcatgatgc aacagattga ggaaccctgc tccctagggg tgcatgcagc cgtggtcatc 720 ccacccacct ggatcctgcg ggcccggagg ccccagaaca ccctcaaggc cagcaagaag 780 aaaaa 785 // ID SCPV02062820; SV 1; linear; genomic DNA; STD; VRT; 820 BP. XX AC SCPV02062820; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22417.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-820 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-820 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; c3ef87ba0449e8fb31f895b46a7667b9. DR ENA-CON; ML191234. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..820 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22417.1" XX SQ Sequence 820 BP; 170 A; 227 C; 264 G; 159 T; 0 other; ggagtgttca gagggctgcc cagtatctga aggtggagca gctctttggg ctgggtcttc 60 ggccacgggt ggagtgccac aaggtcctag agatgttcga ctggaaccta gagcaagccg 120 gctgtcacct tctgggctcc tgtggccctg ctcatcacaa acgctgatgg ttgactggag 180 agcagtggtc tgccctaaaa gaacctcttg aacctgtctg tgggacaagg gtgggggagc 240 tgccctgcct caggcccgaa ggacccacaa ctgcctgcgg ctcccagggg acagtgagac 300 ccaggcagca ggaggctggg agcctcacct tgcccacctc gctcacccac ccagcgctgt 360 tctgcacacc ttggttcagc cctcagtgcc ccaagaggtg ggacgttccc agccttgtga 420 aggcaagaca ggaagctgcc tggcaggctc tgtgtggcct gcctctggct ccagatcaag 480 gctgggctgg tggctagagt gtgccccagg ctttcctctg ggggattaga ctgacaggaa 540 gccaggcttg acatggacaa gaaggatgtg ttggccacac agcagggcga gccagcaacc 600 aacaggccct cctcagggct aggccccttc ccagggaacc cctgtaggag tgtggagtgt 660 tggacagaat gtgacctgta gcttcactgt gaacttgtgg gcttggctgg tcttaggaac 720 ttgtacctat aaacagcctg aagtggctca gaaacagaaa aggtgccagg atcttctctg 780 gcagggacca gggcccaagg ccccggatgt ggaaggagac 820 // ID SCPV02062821; SV 1; linear; genomic DNA; STD; VRT; 820 BP. XX AC SCPV02062821; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22417.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-820 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-820 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; c3ef87ba0449e8fb31f895b46a7667b9. DR ENA-CON; ML191234. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..820 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22417.2" XX SQ Sequence 820 BP; 170 A; 227 C; 264 G; 159 T; 0 other; ggagtgttca gagggctgcc cagtatctga aggtggagca gctctttggg ctgggtcttc 60 ggccacgggt ggagtgccac aaggtcctag agatgttcga ctggaaccta gagcaagccg 120 gctgtcacct tctgggctcc tgtggccctg ctcatcacaa acgctgatgg ttgactggag 180 agcagtggtc tgccctaaaa gaacctcttg aacctgtctg tgggacaagg gtgggggagc 240 tgccctgcct caggcccgaa ggacccacaa ctgcctgcgg ctcccagggg acagtgagac 300 ccaggcagca ggaggctggg agcctcacct tgcccacctc gctcacccac ccagcgctgt 360 tctgcacacc ttggttcagc cctcagtgcc ccaagaggtg ggacgttccc agccttgtga 420 aggcaagaca ggaagctgcc tggcaggctc tgtgtggcct gcctctggct ccagatcaag 480 gctgggctgg tggctagagt gtgccccagg ctttcctctg ggggattaga ctgacaggaa 540 gccaggcttg acatggacaa gaaggatgtg ttggccacac agcagggcga gccagcaacc 600 aacaggccct cctcagggct aggccccttc ccagggaacc cctgtaggag tgtggagtgt 660 tggacagaat gtgacctgta gcttcactgt gaacttgtgg gcttggctgg tcttaggaac 720 ttgtacctat aaacagcctg aagtggctca gaaacagaaa aggtgccagg atcttctctg 780 gcagggacca gggcccaagg ccccggatgt ggaaggagac 820 // ID SCPV02062822; SV 1; linear; genomic DNA; STD; VRT; 786 BP. XX AC SCPV02062822; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22418.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-786 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-786 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; ca15fb40afb41753cb0dec44243049bc. DR ENA-CON; ML191235. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..786 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22418.1" XX SQ Sequence 786 BP; 246 A; 167 C; 262 G; 111 T; 0 other; aatttgaaac ccagaggagg gtggaggcgg cgggtgcgga gggccggcgg cgtccgaggc 60 tggaacggga cgcgttcccg gggcgagcaa gctcgagagg ggcggtgtcc ggtgtaagcg 120 gtaaccatga ctactgaagt tggctctgca tctgaagtga agaagggctc tgaccaggct 180 ggagcagatg cctccaagga gaaagctaaa gaagtagaaa atgagcagac tccagtatct 240 gagccagagg aagaaaaggg ttcccagcca ggtcctccgg ttgagaggca aagcactccc 300 cgcctgcgga aacgagggaa ggatccgtct gagaacaggg gcatctctcg attcataccc 360 ccgtggctta agaagcagag gtcttacaat ttagtagtag ccaaagatgg aggagataaa 420 aaagagccta ctcaagctga cgtggaagac cagatcttag gcaaagagga atcccttcct 480 gaagaagaga gtcgggccaa gggtgacgca gaagaaatgg ctcagaggaa gcacctggag 540 gttcaggtgg aggtcaggga agcaaaacca gccttgaaaa gcagcgtgga gacccagcct 600 gctgaagaag tgaggaagga caaggaggaa acgatacaag acacacagga agagaaactg 660 gaaggaggtg cagccaaaag ggagacaaag gaagtacaga caagtgagct gaaggcagag 720 gtggcctctc agaaagctac caagaagacc aagactgtcc tggccaaagt gacccttcta 780 gatggc 786 // ID SCPV02062823; SV 1; linear; genomic DNA; STD; VRT; 786 BP. XX AC SCPV02062823; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22418.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-786 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-786 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; ca15fb40afb41753cb0dec44243049bc. DR ENA-CON; ML191235. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..786 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22418.2" XX SQ Sequence 786 BP; 246 A; 167 C; 262 G; 111 T; 0 other; aatttgaaac ccagaggagg gtggaggcgg cgggtgcgga gggccggcgg cgtccgaggc 60 tggaacggga cgcgttcccg gggcgagcaa gctcgagagg ggcggtgtcc ggtgtaagcg 120 gtaaccatga ctactgaagt tggctctgca tctgaagtga agaagggctc tgaccaggct 180 ggagcagatg cctccaagga gaaagctaaa gaagtagaaa atgagcagac tccagtatct 240 gagccagagg aagaaaaggg ttcccagcca ggtcctccgg ttgagaggca aagcactccc 300 cgcctgcgga aacgagggaa ggatccgtct gagaacaggg gcatctctcg attcataccc 360 ccgtggctta agaagcagag gtcttacaat ttagtagtag ccaaagatgg aggagataaa 420 aaagagccta ctcaagctga cgtggaagac cagatcttag gcaaagagga atcccttcct 480 gaagaagaga gtcgggccaa gggtgacgca gaagaaatgg ctcagaggaa gcacctggag 540 gttcaggtgg aggtcaggga agcaaaacca gccttgaaaa gcagcgtgga gacccagcct 600 gctgaagaag tgaggaagga caaggaggaa acgatacaag acacacagga agagaaactg 660 gaaggaggtg cagccaaaag ggagacaaag gaagtacaga caagtgagct gaaggcagag 720 gtggcctctc agaaagctac caagaagacc aagactgtcc tggccaaagt gacccttcta 780 gatggc 786 // ID SCPV02062824; SV 1; linear; genomic DNA; STD; VRT; 797 BP. XX AC SCPV02062824; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22419.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-797 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-797 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 987cdd2682581c2707429eba35e58eb7. DR ENA-CON; ML191236. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..797 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22419.1" XX SQ Sequence 797 BP; 249 A; 150 C; 141 G; 257 T; 0 other; tccctacaca agcggcgact ccagtttgag aaacactggt atataacaac actatgatac 60 acttaaatta ggctatattt ttaaaattag ggttaacgtc caagctttgt atctgcatct 120 gaatctaatc tttcccatat ttcaggttgg ttcaattctg ggtccagttt tccaaaagtt 180 tgggaatgtc cagatcctta gctggtgtaa aatgctacat ccatatgaag ttcaatgaaa 240 ttatgcttat ttacatcagc tgaagatttg gtctccagga ttttagaaga ggaataaata 300 tattcacact aatgcctttg cagcaggctg tttccagatt ttaaaaagac aaaaacacat 360 acatttagca ctctaatacc taggatttct gcagtgattt tcatttcagg ctcttttcaa 420 ggagttcatg tacaatgtaa cagagccaag tttgcactgc atgcaacttt caatttgtat 480 tgatcacagc gatcttccag ggtgaccagg gcattatact tcctcagcta cttgtgcatt 540 agctctgttg ggaagctgct agttattttt agcaagcata gaataattta agcttcaagt 600 tgtaccttta atcttgaaaa gatctcacat actattgtga tgagagctgt ataattacca 660 ataaataatt ccacatatat taagtactct tcacaacatt cagtgacata tatataggaa 720 atattgtcat cctcattata gagctgaaga aactgaggca atgagacatt cagctgactt 780 gtgtgaggcc agacagc 797 // ID SCPV02062825; SV 1; linear; genomic DNA; STD; VRT; 1191 BP. XX AC SCPV02062825; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22419.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-1191 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-1191 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 52b1e90eab0fa06e96922cacb9b26701. DR ENA-CON; ML191236. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1191 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22419.2" XX SQ Sequence 1191 BP; 317 A; 298 C; 257 G; 319 T; 0 other; ggcaatgaga cattcagctg acttgtgtga ggccagacag caagccaatg gcagaactgc 60 ggttagatag caatcatgag agcaacagta gaactggcac atactgtctg gcagctaggg 120 gggcagtaac tgggtttatc aaatcatctt cttgctttca ttttactaat gtggcaaatt 180 gccagcacta ttatgatgga tatcacactt tctcttcttt tggggggggg tttcagggca 240 ccatttcttg cccctgaatt ggaatattaa ttgccccact agtgtcctag aggaggggag 300 tggagaggga gggacctggg ctcgtcctct actccaggtc ccagcccagg agccctgagg 360 atagtggtga accacttgaa ctggaggttc cttccgctgg gctacttccc tctcctgccc 420 ttcagcttgt ggggagactt cctgccctcc ctctgcacaa gccaagtgcc ccttacctag 480 ggtctaggac ttcttagccc accgcagcac ttctccaaac tttcctctgc tttccttcaa 540 actgttctct gctccaatac caatccttta tgctccaact cccacactgg ctgactgaag 600 caggggtttt ttatcatgtg actgactgca ggtgctctaa ttggctttag gtgctctagt 660 taatctatag caaactttct tccccttaca aggaataagg ctcctttcta accctctcct 720 gctgtcctct ggccatgctg tatcacacta actagagcta tagtatcaaa gacaagggca 780 gattttccta tttaagcaag aatattctcc ctctaaactt acacacccat agttatttaa 840 ttccttattc tctaatgaat cagcagcaga atgtatccaa gaagcataca gttactgtag 900 tagtaagaga ccctgaaaca tgaggccttg tatcagaggc ctgagacctg aactaaaata 960 gtggtgaaac ctttctgaca tatagcaaat ttaccaaaag tcaggctgtg agcagatgtc 1020 aggatctgcc tgcgtgcagg ctctcagaac ccagcaagaa cagggctggt attacaaaaa 1080 tacacatgat cctaagaagt gctaattctg ggaccatcat gcaagcacat tccagaaggg 1140 tagtaccaag tatggtatga acacattccc caaagatcat ataaagacac a 1191 // ID SCPV02062826; SV 1; linear; genomic DNA; STD; VRT; 718 BP. XX AC SCPV02062826; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22420.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-718 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-718 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 182fd6ff6cb45aa822e643b57f7bbde9. DR ENA-CON; ML191237. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..718 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22420.1" XX SQ Sequence 718 BP; 232 A; 142 C; 163 G; 181 T; 0 other; gggagtgtcg ccgccatctt tgttgatgtg tcagctccgc aggggtttgg ggaaacggcc 60 gctgagtgag gcgtcggctg tgtttctcac cgcggtcttt tcctcccact cttggctggt 120 tggaccccgc tatggaaaag ttggcccctg agccagagct ccagcagcct tgttagggcg 180 tggcctgagg cttggataag tgggatgtaa aacgaagatc aggagcagat ttgaagaatt 240 acaaagtgaa ttggtgccag tcagcatgtc agagacagac cacatagcct ctacttcctc 300 tgataaaaat gttgggaaaa cacctgaatt aaaggaagac tcatgcaact tgttttctgg 360 caatgaaagc agcaaattag aaaatgagtc caaactattg tcattaaaca ctgataaaac 420 tttatgtcaa cctaatgagc ataataatcg aattgaagcc caggaaaatt atattccaga 480 tcatggtgga ggtgaggatt cttgtgccaa aacagacaca ggctcagaaa attctgaaca 540 aatagctaat tttcctagtg gaaattttgc taaacatatt tcaaaaacaa atgaaacaga 600 acagaaagta acacaaatat tggtggaatt aaggtcatct acatttccag aatcagctaa 660 tgaaaagact tattcagaaa gcccctatga tacagactgc accaagaaat ttatttca 718 // ID SCPV02062827; SV 1; linear; genomic DNA; STD; VRT; 718 BP. XX AC SCPV02062827; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22420.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-718 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-718 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 182fd6ff6cb45aa822e643b57f7bbde9. DR ENA-CON; ML191237. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..718 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22420.2" XX SQ Sequence 718 BP; 232 A; 142 C; 163 G; 181 T; 0 other; gggagtgtcg ccgccatctt tgttgatgtg tcagctccgc aggggtttgg ggaaacggcc 60 gctgagtgag gcgtcggctg tgtttctcac cgcggtcttt tcctcccact cttggctggt 120 tggaccccgc tatggaaaag ttggcccctg agccagagct ccagcagcct tgttagggcg 180 tggcctgagg cttggataag tgggatgtaa aacgaagatc aggagcagat ttgaagaatt 240 acaaagtgaa ttggtgccag tcagcatgtc agagacagac cacatagcct ctacttcctc 300 tgataaaaat gttgggaaaa cacctgaatt aaaggaagac tcatgcaact tgttttctgg 360 caatgaaagc agcaaattag aaaatgagtc caaactattg tcattaaaca ctgataaaac 420 tttatgtcaa cctaatgagc ataataatcg aattgaagcc caggaaaatt atattccaga 480 tcatggtgga ggtgaggatt cttgtgccaa aacagacaca ggctcagaaa attctgaaca 540 aatagctaat tttcctagtg gaaattttgc taaacatatt tcaaaaacaa atgaaacaga 600 acagaaagta acacaaatat tggtggaatt aaggtcatct acatttccag aatcagctaa 660 tgaaaagact tattcagaaa gcccctatga tacagactgc accaagaaat ttatttca 718 // ID SCPV02062828; SV 1; linear; genomic DNA; STD; VRT; 539 BP. XX AC SCPV02062828; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22421.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-539 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-539 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4950f5df12ce889f6b0a7b07cb88a432. DR ENA-CON; ML191238. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..539 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22421.1" XX SQ Sequence 539 BP; 91 A; 158 C; 182 G; 108 T; 0 other; gcgccgaggg gcgcgcgtgg ccgaggggct tggattcctt gcctggagga gcattacgtg 60 acacagcaag gctccgcaca tcctagtcgc ctgcggggga cgcaacgctg ctcgctccgg 120 ctagtcactg gcgttgcctg ccctctgccg gcctgcggat ggttcggtcc ggctgggtca 180 cgcgctaagg ctctgtgtaa ttgcgggcgg aacgggctag cacttgccga ggggcagaac 240 agtggcgaca gccccaggac agttgcgagc gggttccggc ccagcatccg ggagacggcg 300 gcaagcgccc cagctgggtt ggtgttgggc catagggctg aatggaaagc gcggatgaca 360 acctcaagtc tttgaattcg aatagtgcag cttgcttacc tgactcttcc ggcgggcctc 420 gtacatgttc tgctctaggc gggatggtgc agttgtgatg tgctaagcat aaggcctcgg 480 ccattctcca gcaccatgaa cgccatagtc gccctctgcc acttctgcga gctccatgg 539 // ID SCPV02062829; SV 1; linear; genomic DNA; STD; VRT; 539 BP. XX AC SCPV02062829; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22421.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-539 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-539 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4950f5df12ce889f6b0a7b07cb88a432. DR ENA-CON; ML191238. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..539 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22421.2" XX SQ Sequence 539 BP; 91 A; 158 C; 182 G; 108 T; 0 other; gcgccgaggg gcgcgcgtgg ccgaggggct tggattcctt gcctggagga gcattacgtg 60 acacagcaag gctccgcaca tcctagtcgc ctgcggggga cgcaacgctg ctcgctccgg 120 ctagtcactg gcgttgcctg ccctctgccg gcctgcggat ggttcggtcc ggctgggtca 180 cgcgctaagg ctctgtgtaa ttgcgggcgg aacgggctag cacttgccga ggggcagaac 240 agtggcgaca gccccaggac agttgcgagc gggttccggc ccagcatccg ggagacggcg 300 gcaagcgccc cagctgggtt ggtgttgggc catagggctg aatggaaagc gcggatgaca 360 acctcaagtc tttgaattcg aatagtgcag cttgcttacc tgactcttcc ggcgggcctc 420 gtacatgttc tgctctaggc gggatggtgc agttgtgatg tgctaagcat aaggcctcgg 480 ccattctcca gcaccatgaa cgccatagtc gccctctgcc acttctgcga gctccatgg 539 // ID SCPV02062830; SV 1; linear; genomic DNA; STD; VRT; 860 BP. XX AC SCPV02062830; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22422.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-860 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-860 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d87f1f728fa60d3569acf2de46097e3f. DR ENA-CON; ML191239. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..860 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22422.1" XX SQ Sequence 860 BP; 263 A; 165 C; 178 G; 254 T; 0 other; aggctaccca aaactttgag gaggaagaat tgatcctgtg acaggctagt ctccactgat 60 tttcatcaat catgaggcag ttcaagatgg ttatgcttac ggggatgtcc agaaatttct 120 ctccttctgc tcggatattg tatttgcagg gtcgagtaga ctagactgat tatgatcaaa 180 gaggagacat ttcatctgaa ggtacatttg aaatacaggg ttgatttttc tgggatgttt 240 gtttgtttgt ttgtttgttt tttaatctgc taaactaaat tatcaattaa aggagttcat 300 agagcataga acctcatttc tgaattatat gtggatcctg ggatggaaaa atactgagga 360 gaaaggtttc cctccacctc catgaaataa ttacaatttc ttgttcatat agtttcctga 420 gtccatgagc attgggaata aaggggaagt ttaaattctt atcctcaagg ctcaatagct 480 gcataaatca acatggattt attcttttgt ctctagcttc aggttctgct cctcgtatct 540 ttgttgagga ttaccaggat ggagggatcc gagtggtatg ccgatcagct ggatggtacc 600 cagagcccga agtgctgtgg agagatcaca agggacagcc cttaccttca tcctctctga 660 caaattcccg agaagccagc agactatata aaacagaaac ttctatcatt ctaacagaaa 720 attcaaatca gaacatgtcc tgtttggtca ggaacaccca cctcagccta gaaaaggaat 780 cattatttta tatatcaggt cagtaaccat caaaaaccac acactgaaca ggaacagaaa 840 aaaataagat gacaaccctg 860 // ID SCPV02062831; SV 1; linear; genomic DNA; STD; VRT; 860 BP. XX AC SCPV02062831; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22422.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-860 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-860 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d87f1f728fa60d3569acf2de46097e3f. DR ENA-CON; ML191239. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..860 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22422.2" XX SQ Sequence 860 BP; 263 A; 165 C; 178 G; 254 T; 0 other; aggctaccca aaactttgag gaggaagaat tgatcctgtg acaggctagt ctccactgat 60 tttcatcaat catgaggcag ttcaagatgg ttatgcttac ggggatgtcc agaaatttct 120 ctccttctgc tcggatattg tatttgcagg gtcgagtaga ctagactgat tatgatcaaa 180 gaggagacat ttcatctgaa ggtacatttg aaatacaggg ttgatttttc tgggatgttt 240 gtttgtttgt ttgtttgttt tttaatctgc taaactaaat tatcaattaa aggagttcat 300 agagcataga acctcatttc tgaattatat gtggatcctg ggatggaaaa atactgagga 360 gaaaggtttc cctccacctc catgaaataa ttacaatttc ttgttcatat agtttcctga 420 gtccatgagc attgggaata aaggggaagt ttaaattctt atcctcaagg ctcaatagct 480 gcataaatca acatggattt attcttttgt ctctagcttc aggttctgct cctcgtatct 540 ttgttgagga ttaccaggat ggagggatcc gagtggtatg ccgatcagct ggatggtacc 600 cagagcccga agtgctgtgg agagatcaca agggacagcc cttaccttca tcctctctga 660 caaattcccg agaagccagc agactatata aaacagaaac ttctatcatt ctaacagaaa 720 attcaaatca gaacatgtcc tgtttggtca ggaacaccca cctcagccta gaaaaggaat 780 cattatttta tatatcaggt cagtaaccat caaaaaccac acactgaaca ggaacagaaa 840 aaaataagat gacaaccctg 860 // ID SCPV02062832; SV 1; linear; genomic DNA; STD; VRT; 762 BP. XX AC SCPV02062832; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22423.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-762 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-762 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 50724016a36c5946731b5f6be90825cd. DR ENA-CON; ML191240. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..762 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22423.1" XX SQ Sequence 762 BP; 186 A; 174 C; 210 G; 192 T; 0 other; ggatagtgtc ctcactgatt gtgccatctc tgctgggacc aacagtggtc acaggaggct 60 gggaagggta ctgtgagctt gttgtggcag ggtatgggcc agcaggtggg taaggacagc 120 caggataacc actggggttg ggagggtatc cagatggata tgcagagatt ccacttggca 180 tgcctggcat gtaggaggta tttggtggcc ctgttgctgt gtatggtggg taggatgcag 240 aaacagtagg ccgggagaac actggaggct cctctccaaa tatcacaatc atgatttgaa 300 taagctccag caactctgac cgtggatgtt tccagtcatg tagataaggt aggtagattt 360 tcccatttgc atccacatgc tttcctgttt taatagtcat tgaactagta ggcttaacaa 420 aacagatagg ggggttatat gggtatgtgt ccagcagcca caggcatatt ggaatattat 480 atatattacc tcgataacgc actgggattg taccagtgag gttcaccagc tccctggaac 540 tgccatcatt aaaaacatat gaatccaata caggtttgag atctttgtac atagcgatga 600 cattgacagt ttgacggacg gttagatctc tatatttgta cttggacatc atcttcttca 660 gctgactctc ggacaccgcc atggcgcgct cctccgtccc cacaggcaga gggtaggcgc 720 cacggggccg ccccaggccg ccagacaatc gccgccgccc cc 762 // ID SCPV02062833; SV 1; linear; genomic DNA; STD; VRT; 762 BP. XX AC SCPV02062833; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22423.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-762 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-762 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 50724016a36c5946731b5f6be90825cd. DR ENA-CON; ML191240. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..762 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22423.2" XX SQ Sequence 762 BP; 186 A; 174 C; 210 G; 192 T; 0 other; ggatagtgtc ctcactgatt gtgccatctc tgctgggacc aacagtggtc acaggaggct 60 gggaagggta ctgtgagctt gttgtggcag ggtatgggcc agcaggtggg taaggacagc 120 caggataacc actggggttg ggagggtatc cagatggata tgcagagatt ccacttggca 180 tgcctggcat gtaggaggta tttggtggcc ctgttgctgt gtatggtggg taggatgcag 240 aaacagtagg ccgggagaac actggaggct cctctccaaa tatcacaatc atgatttgaa 300 taagctccag caactctgac cgtggatgtt tccagtcatg tagataaggt aggtagattt 360 tcccatttgc atccacatgc tttcctgttt taatagtcat tgaactagta ggcttaacaa 420 aacagatagg ggggttatat gggtatgtgt ccagcagcca caggcatatt ggaatattat 480 atatattacc tcgataacgc actgggattg taccagtgag gttcaccagc tccctggaac 540 tgccatcatt aaaaacatat gaatccaata caggtttgag atctttgtac atagcgatga 600 cattgacagt ttgacggacg gttagatctc tatatttgta cttggacatc atcttcttca 660 gctgactctc ggacaccgcc atggcgcgct cctccgtccc cacaggcaga gggtaggcgc 720 cacggggccg ccccaggccg ccagacaatc gccgccgccc cc 762 // ID SCPV02062834; SV 1; linear; genomic DNA; STD; VRT; 597 BP. XX AC SCPV02062834; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22424.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-597 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-597 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2fdb761f2842a2c198f98f97ef6921ac. DR ENA-CON; ML191241. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..597 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22424.1" XX SQ Sequence 597 BP; 205 A; 133 C; 124 G; 135 T; 0 other; ccacatatgc ttgtgcttgg gccatgctac ggtcaggaag aactggcgga ggcccttagc 60 cttcacctct gagtgcccca gagattgtgc aagcaggaag tttaggttaa ggtagagttg 120 tcaacctttt ggctgagtgt tgaaggcagg ccccaacaag caaccagtgg tatgaggcag 180 gtaacttgct ggttccaggc atttaggaaa atctgtgtcc atcctcttac ctgatcagta 240 agccaagtag atgtgttaat agtcacacac ataatacaca cacacacaca cacacacaca 300 cacacacccc acagacttcc catattagtt ttgaaaagtt actaaacaac aattataagg 360 gctcctgggg agaatctgat ttccaaagat gccatgttgt gttaagatag ccagatttta 420 acagcagtac aagacacaca gagctacagt aataaacaca gaggaaataa aaaaagatcc 480 tgggctaact atctaggcaa aacccttaga tcatctattt caaaaacatt caaagaactg 540 atcggaagca tgtctaaaaa ataaaccaag acaaaagtag gagaatgatg tgcccca 597 // ID SCPV02062835; SV 1; linear; genomic DNA; STD; VRT; 597 BP. XX AC SCPV02062835; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22424.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-597 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-597 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2fdb761f2842a2c198f98f97ef6921ac. DR ENA-CON; ML191241. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..597 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22424.2" XX SQ Sequence 597 BP; 205 A; 133 C; 124 G; 135 T; 0 other; ccacatatgc ttgtgcttgg gccatgctac ggtcaggaag aactggcgga ggcccttagc 60 cttcacctct gagtgcccca gagattgtgc aagcaggaag tttaggttaa ggtagagttg 120 tcaacctttt ggctgagtgt tgaaggcagg ccccaacaag caaccagtgg tatgaggcag 180 gtaacttgct ggttccaggc atttaggaaa atctgtgtcc atcctcttac ctgatcagta 240 agccaagtag atgtgttaat agtcacacac ataatacaca cacacacaca cacacacaca 300 cacacacccc acagacttcc catattagtt ttgaaaagtt actaaacaac aattataagg 360 gctcctgggg agaatctgat ttccaaagat gccatgttgt gttaagatag ccagatttta 420 acagcagtac aagacacaca gagctacagt aataaacaca gaggaaataa aaaaagatcc 480 tgggctaact atctaggcaa aacccttaga tcatctattt caaaaacatt caaagaactg 540 atcggaagca tgtctaaaaa ataaaccaag acaaaagtag gagaatgatg tgcccca 597 // ID SCPV02062836; SV 1; linear; genomic DNA; STD; VRT; 838 BP. XX AC SCPV02062836; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22425.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-838 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-838 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2beaf0cb9d7474912af291003c3a9888. DR ENA-CON; ML191242. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..838 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22425.1" XX SQ Sequence 838 BP; 160 A; 280 C; 224 G; 174 T; 0 other; gggggatccg agctgccggg gcaggtgctt cggccccatc cacacaggcc acctgcaatg 60 gcccaccaaa gtccagcact acccaactgg gagtttcagg ggggcaggga gctcccacac 120 caccccccac ctcccaggac ccccttcctg tatctggacc agtgacagct cctgttccca 180 ggcccctggg ctccattcag agaccaaaca gcttcctctt ccggtcctct tctcagagtg 240 gctctagtcc ttcctctcca gagtctgttt tgagacctcg gccgtttcct caggagaagg 300 agctaatatc ccaacttcgc caggtcctag agtcgcggct gcagcagccc ctacctgagg 360 acctggcaga agctctcgcc aacggagtcc tcctttgcca gctggcgaac cagctgcggc 420 cccgctctgt acccttcatt catgtgccct cacctgctgt gccaaagctc agtgctctca 480 agtctcggaa gaatgtcgag agtttcctag aagcctgtcg gaaaatgggt gtgcctgagg 540 agtccctgtg ccagccccac cacatcctgg aagaggaagg ggcccctgga aggggcctcc 600 ctcacatcgc tgctgtcctt cacgcacttc tggaacagcc ttaggggtag aggttggggg 660 ctctgggccc caactaccat cggacctgtc ctgggagcag atgacacctg ggcccagcca 720 attaacaagg acttggtgac catggtcttg tgtgtagcac tggccttgga tatccccctg 780 cccttaggct gcttcgggta cagcccccat ctgggtgaag agcgttgtgt caaacaca 838 // ID SCPV02062837; SV 1; linear; genomic DNA; STD; VRT; 838 BP. XX AC SCPV02062837; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22425.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-838 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-838 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2beaf0cb9d7474912af291003c3a9888. DR ENA-CON; ML191242. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..838 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22425.2" XX SQ Sequence 838 BP; 160 A; 280 C; 224 G; 174 T; 0 other; gggggatccg agctgccggg gcaggtgctt cggccccatc cacacaggcc acctgcaatg 60 gcccaccaaa gtccagcact acccaactgg gagtttcagg ggggcaggga gctcccacac 120 caccccccac ctcccaggac ccccttcctg tatctggacc agtgacagct cctgttccca 180 ggcccctggg ctccattcag agaccaaaca gcttcctctt ccggtcctct tctcagagtg 240 gctctagtcc ttcctctcca gagtctgttt tgagacctcg gccgtttcct caggagaagg 300 agctaatatc ccaacttcgc caggtcctag agtcgcggct gcagcagccc ctacctgagg 360 acctggcaga agctctcgcc aacggagtcc tcctttgcca gctggcgaac cagctgcggc 420 cccgctctgt acccttcatt catgtgccct cacctgctgt gccaaagctc agtgctctca 480 agtctcggaa gaatgtcgag agtttcctag aagcctgtcg gaaaatgggt gtgcctgagg 540 agtccctgtg ccagccccac cacatcctgg aagaggaagg ggcccctgga aggggcctcc 600 ctcacatcgc tgctgtcctt cacgcacttc tggaacagcc ttaggggtag aggttggggg 660 ctctgggccc caactaccat cggacctgtc ctgggagcag atgacacctg ggcccagcca 720 attaacaagg acttggtgac catggtcttg tgtgtagcac tggccttgga tatccccctg 780 cccttaggct gcttcgggta cagcccccat ctgggtgaag agcgttgtgt caaacaca 838 // ID SCPV02062838; SV 1; linear; genomic DNA; STD; VRT; 868 BP. XX AC SCPV02062838; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22426.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-868 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-868 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f1803dd788edc143c63508c397d4bf16. DR ENA-CON; ML191243. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..868 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22426.1" XX SQ Sequence 868 BP; 124 A; 263 C; 197 G; 284 T; 0 other; tgaggctggt gattctcatc aggccatcct cctcctcgta gtccgattca ctctcttcac 60 tgtcagatct gggtgcaact tcgtaaccag gattcatctc cagccaggca tccagctgac 120 ttgctttggg tgcttctggt ccaaagagga ccttgccagt ttctgtgtgt gtcactttga 180 caggcaggtc actcatctgg ctgctttcat ctattggctc tccatctggt cccagggcag 240 gttcccctcc ctctgcattc tcttcagcct tcttcttcct cctcctcctc ttcttcttct 300 ctttggctgc ttgggcctgc ttgtgctccc acaccaggtt ggtcagattg gcgacatact 360 catcggtctg ctgcaatagg taggcgagac gtctgtcttt cttttggtca ataagcttcc 420 tgtagccctc ttcatcttcg gccatcagcc tccgcattct ctccttctcg atccgctccg 480 tctccttctt ctgctccctt tctgtgttag catgccaagt cgccactgct ttggagagct 540 tctggatctt cccggccaca gaccggtgat attccttaaa atcttttgca tgttgcaaaa 600 tactgttcag gtattcctgg tgtttctgcc ggcgtttcct ctcctgttct atcttctgct 660 gcttctccag tttctctgtc atgcgtgcct cacgcagggt ctggcgcttg ctccgcttat 720 atgctttgga gttgagggcc gtctccaggg tggtgtccct ccgcatgcag gccaccacct 780 cctgtctcag ctgacgttgg aagttgagta agcgaagtgc tttcagttcc acggttgctt 840 tggtgcgtaa atctggtggc aaggaacc 868 // ID SCPV02062839; SV 1; linear; genomic DNA; STD; VRT; 868 BP. XX AC SCPV02062839; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22426.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-868 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-868 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f1803dd788edc143c63508c397d4bf16. DR ENA-CON; ML191243. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..868 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22426.2" XX SQ Sequence 868 BP; 124 A; 263 C; 197 G; 284 T; 0 other; tgaggctggt gattctcatc aggccatcct cctcctcgta gtccgattca ctctcttcac 60 tgtcagatct gggtgcaact tcgtaaccag gattcatctc cagccaggca tccagctgac 120 ttgctttggg tgcttctggt ccaaagagga ccttgccagt ttctgtgtgt gtcactttga 180 caggcaggtc actcatctgg ctgctttcat ctattggctc tccatctggt cccagggcag 240 gttcccctcc ctctgcattc tcttcagcct tcttcttcct cctcctcctc ttcttcttct 300 ctttggctgc ttgggcctgc ttgtgctccc acaccaggtt ggtcagattg gcgacatact 360 catcggtctg ctgcaatagg taggcgagac gtctgtcttt cttttggtca ataagcttcc 420 tgtagccctc ttcatcttcg gccatcagcc tccgcattct ctccttctcg atccgctccg 480 tctccttctt ctgctccctt tctgtgttag catgccaagt cgccactgct ttggagagct 540 tctggatctt cccggccaca gaccggtgat attccttaaa atcttttgca tgttgcaaaa 600 tactgttcag gtattcctgg tgtttctgcc ggcgtttcct ctcctgttct atcttctgct 660 gcttctccag tttctctgtc atgcgtgcct cacgcagggt ctggcgcttg ctccgcttat 720 atgctttgga gttgagggcc gtctccaggg tggtgtccct ccgcatgcag gccaccacct 780 cctgtctcag ctgacgttgg aagttgagta agcgaagtgc tttcagttcc acggttgctt 840 tggtgcgtaa atctggtggc aaggaacc 868 // ID SCPV02062840; SV 1; linear; genomic DNA; STD; VRT; 905 BP. XX AC SCPV02062840; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22427.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-905 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-905 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 18d367b8feccb14529a97808d20bbd4e. DR ENA-CON; ML191244. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..905 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22427.1" XX SQ Sequence 905 BP; 262 A; 207 C; 213 G; 223 T; 0 other; cttgaaactg aacccagctc ttctgcatct cacagcagtt tcttaacccc aagactatcc 60 ttgtggtagc cactgccttg gacctgggct cctccacccc gtcagtccat atggaggggg 120 gctgagctgc tggtgacacc cgcttgcccg cctccccttt ctcaaaggga acttgggcca 180 gcagaagact ctgtgaaaac aacctgcctg gtgcatgtga gcctaactct gagccaggaa 240 ggaggaaggg agggagggag acagatggta tcttgtactg atgccaaaat ttctaggaaa 300 gcttcttcaa ggaaactgac aacaccaaag agaagaagct gccctgccac atgggagact 360 tcccacatga gccggagcag ggaatgccat ggagaccagc ccagggccca aggatgggca 420 ctttctccta ccatgcagga gctctgggtt cagatcctga actggccggg gctgagtgct 480 ggagcgtggt ccatatacag cctgctatgc aatagctggg tttggaacca aagccaggag 540 gagatgggcg cgcttcggaa ctggctccgg acccaaggag tcgtgttcct gttgcagagc 600 cagccaaaaa tgtttttttt catccaaaaa ggaaactttg ctgggaggtt ttaaattgga 660 ttgaaatttt ccacttttta aagttaaaac tgaaaatttt caggaacgac cttgggtttt 720 ggtaaaagcc ttcagttttt gaaaaccaat ctttttttta aatttatttt tgtctgaaaa 780 ttgttgctga aaatgttttt caaaaccaaa agaaatagga aaaacaaatt gggaaattaa 840 aataaataac taaataaata aaaaatctcc cctcccccat gtaaacatta ttttcccaca 900 aaaaa 905 // ID SCPV02062841; SV 1; linear; genomic DNA; STD; VRT; 905 BP. XX AC SCPV02062841; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22427.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-905 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-905 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 18d367b8feccb14529a97808d20bbd4e. DR ENA-CON; ML191244. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..905 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22427.2" XX SQ Sequence 905 BP; 262 A; 207 C; 213 G; 223 T; 0 other; cttgaaactg aacccagctc ttctgcatct cacagcagtt tcttaacccc aagactatcc 60 ttgtggtagc cactgccttg gacctgggct cctccacccc gtcagtccat atggaggggg 120 gctgagctgc tggtgacacc cgcttgcccg cctccccttt ctcaaaggga acttgggcca 180 gcagaagact ctgtgaaaac aacctgcctg gtgcatgtga gcctaactct gagccaggaa 240 ggaggaaggg agggagggag acagatggta tcttgtactg atgccaaaat ttctaggaaa 300 gcttcttcaa ggaaactgac aacaccaaag agaagaagct gccctgccac atgggagact 360 tcccacatga gccggagcag ggaatgccat ggagaccagc ccagggccca aggatgggca 420 ctttctccta ccatgcagga gctctgggtt cagatcctga actggccggg gctgagtgct 480 ggagcgtggt ccatatacag cctgctatgc aatagctggg tttggaacca aagccaggag 540 gagatgggcg cgcttcggaa ctggctccgg acccaaggag tcgtgttcct gttgcagagc 600 cagccaaaaa tgtttttttt catccaaaaa ggaaactttg ctgggaggtt ttaaattgga 660 ttgaaatttt ccacttttta aagttaaaac tgaaaatttt caggaacgac cttgggtttt 720 ggtaaaagcc ttcagttttt gaaaaccaat ctttttttta aatttatttt tgtctgaaaa 780 ttgttgctga aaatgttttt caaaaccaaa agaaatagga aaaacaaatt gggaaattaa 840 aataaataac taaataaata aaaaatctcc cctcccccat gtaaacatta ttttcccaca 900 aaaaa 905 // ID SCPV02062842; SV 1; linear; genomic DNA; STD; VRT; 700 BP. XX AC SCPV02062842; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22428.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-700 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-700 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 49cbe2776222cd7250445faad9f02d49. DR ENA-CON; ML191245. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..700 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22428.1" XX SQ Sequence 700 BP; 167 A; 209 C; 170 G; 154 T; 0 other; tttcttatat gggatactgt ctttaatccc accactgaga gactgggcga gaagacagga 60 ggatctcttg ataggaggca acctggtcta cattgtgatt tccatgctag ctagagagac 120 accgcctctc cccagcccag cagtcctcat ctgtattctg taattacccc cattttccag 180 tttctgacat cctggtgggt ttagttgctc tacatactcc tattgtctac actagtcctg 240 ccaccactcc cttccaagac gtctatccgg atgcccaaac tatggggatg gcaagtaaac 300 gcacatgctc ggtagtgccc aatacccccg cttcttacct gcacatcctg gaagagtgca 360 cggcccccgc gatcgttctg aaactcgagg agacgctccg cgccctcgcg cttctcctcg 420 gccaattcgc ggaagaagtg gcctacgccc tccagagcca cgtcatcccg atcaaaaaag 480 aagccctgcg ggaggttaca aatacgagtt aacggaacgc ggtggctcgg ctcacactgt 540 aaacttcaat cgcaacactc aagaggtaga ggcagtacgt gtgagatttc gagggcagcc 600 cggtctgccc agcgagttcc agtcagacaa aactactatg tacacagcgc gcagggagag 660 ggagaggtcg gagcacagtc tctgccctgc atggaacccc 700 // ID SCPV02062843; SV 1; linear; genomic DNA; STD; VRT; 700 BP. XX AC SCPV02062843; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22428.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-700 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-700 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 49cbe2776222cd7250445faad9f02d49. DR ENA-CON; ML191245. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..700 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22428.2" XX SQ Sequence 700 BP; 167 A; 209 C; 170 G; 154 T; 0 other; tttcttatat gggatactgt ctttaatccc accactgaga gactgggcga gaagacagga 60 ggatctcttg ataggaggca acctggtcta cattgtgatt tccatgctag ctagagagac 120 accgcctctc cccagcccag cagtcctcat ctgtattctg taattacccc cattttccag 180 tttctgacat cctggtgggt ttagttgctc tacatactcc tattgtctac actagtcctg 240 ccaccactcc cttccaagac gtctatccgg atgcccaaac tatggggatg gcaagtaaac 300 gcacatgctc ggtagtgccc aatacccccg cttcttacct gcacatcctg gaagagtgca 360 cggcccccgc gatcgttctg aaactcgagg agacgctccg cgccctcgcg cttctcctcg 420 gccaattcgc ggaagaagtg gcctacgccc tccagagcca cgtcatcccg atcaaaaaag 480 aagccctgcg ggaggttaca aatacgagtt aacggaacgc ggtggctcgg ctcacactgt 540 aaacttcaat cgcaacactc aagaggtaga ggcagtacgt gtgagatttc gagggcagcc 600 cggtctgccc agcgagttcc agtcagacaa aactactatg tacacagcgc gcagggagag 660 ggagaggtcg gagcacagtc tctgccctgc atggaacccc 700 // ID SCPV02062844; SV 1; linear; genomic DNA; STD; VRT; 568 BP. XX AC SCPV02062844; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22429.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-568 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-568 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 61d003a25aa0207eed978a79efa25413. DR ENA-CON; ML191246. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..568 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22429.1" XX SQ Sequence 568 BP; 101 A; 208 C; 202 G; 57 T; 0 other; ggccacagcg ctgggtagaa gaggcactcc cgccagtgcg agcaggaggc cgaggcccgc 60 agccggtcgg gcgccggcag aaaggagaag atgtgcacga tgagctcgct gggcagcgac 120 gcggcgccgg ccgcctgccc gcacagagcc atccggctcg gccgccggcc gcccgtcccc 180 ggccgcctgc gcagtcctcg cagcagcccc cgcagccggc gcagctgcag cagccgcagc 240 cgctgccgcc gccgccaccg caccaaccgg gcggccccgg ctcgggttcg cgctcccggc 300 ggtcggggtt gcggcactga caagaacaac aacatcaatg acaaggaaga agggggcgga 360 accgtcgtgc gcctcggcgg aaccaagtgc actcggaccg gctgagaggg gggaaaagct 420 ggcgcgggga ggcctgcacg ccgctgactt tccgtaccgc aagcaagtgc tccccggccg 480 gcccgggcaa cgacctcgaa atcctctctc gaacggacca gggatgcgga ggcgaccgcg 540 ctgcttccga ggaaaccgtg gttctccc 568 // ID SCPV02062845; SV 1; linear; genomic DNA; STD; VRT; 568 BP. XX AC SCPV02062845; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22429.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-568 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-568 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 61d003a25aa0207eed978a79efa25413. DR ENA-CON; ML191246. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..568 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22429.2" XX SQ Sequence 568 BP; 101 A; 208 C; 202 G; 57 T; 0 other; ggccacagcg ctgggtagaa gaggcactcc cgccagtgcg agcaggaggc cgaggcccgc 60 agccggtcgg gcgccggcag aaaggagaag atgtgcacga tgagctcgct gggcagcgac 120 gcggcgccgg ccgcctgccc gcacagagcc atccggctcg gccgccggcc gcccgtcccc 180 ggccgcctgc gcagtcctcg cagcagcccc cgcagccggc gcagctgcag cagccgcagc 240 cgctgccgcc gccgccaccg caccaaccgg gcggccccgg ctcgggttcg cgctcccggc 300 ggtcggggtt gcggcactga caagaacaac aacatcaatg acaaggaaga agggggcgga 360 accgtcgtgc gcctcggcgg aaccaagtgc actcggaccg gctgagaggg gggaaaagct 420 ggcgcgggga ggcctgcacg ccgctgactt tccgtaccgc aagcaagtgc tccccggccg 480 gcccgggcaa cgacctcgaa atcctctctc gaacggacca gggatgcgga ggcgaccgcg 540 ctgcttccga ggaaaccgtg gttctccc 568 // ID SCPV02062846; SV 1; linear; genomic DNA; STD; VRT; 522 BP. XX AC SCPV02062846; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22430.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-522 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-522 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; dc0b8687e86984619a6fe2c494c8d414. DR ENA-CON; ML191247. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..522 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22430.1" XX SQ Sequence 522 BP; 130 A; 149 C; 115 G; 128 T; 0 other; ctcaggaggc atcgaacaac tggaccaaca agattccacc ccatagactt aatgatggtc 60 ttgttctttt catcttgaat aatctctagc agctggttta tataaccatc ttcaatgcat 120 ctttgacctg ctaactgaaa gaggccaaaa tcttcttctt taaaatcatg ctcttctagg 180 atttgacatc tttttattat gctctgaagt tcctccacag ccattcctaa ttattttcct 240 aattttgatg ctgtaatgaa ataaacaatc gtcagtgaga tcaccgagta tcacgaaaag 300 gaggccagca gcgaaggtcc ttgccccgca ggcgcgaaca ggaaaatcac accctaccgc 360 gcctggctga ggcgaaggga ggccatttct ccctgctcct gcagggcagg gtgtccaggc 420 tccagcacct ccagaggccg agcaggagag ccgcgtgcgg cgcaggtcct gttcggcgag 480 cccgatccgc ccgacccagc atgtctacct acccgccgat cc 522 // ID SCPV02062847; SV 1; linear; genomic DNA; STD; VRT; 522 BP. XX AC SCPV02062847; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22430.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-522 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-522 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; dc0b8687e86984619a6fe2c494c8d414. DR ENA-CON; ML191247. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..522 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22430.2" XX SQ Sequence 522 BP; 130 A; 149 C; 115 G; 128 T; 0 other; ctcaggaggc atcgaacaac tggaccaaca agattccacc ccatagactt aatgatggtc 60 ttgttctttt catcttgaat aatctctagc agctggttta tataaccatc ttcaatgcat 120 ctttgacctg ctaactgaaa gaggccaaaa tcttcttctt taaaatcatg ctcttctagg 180 atttgacatc tttttattat gctctgaagt tcctccacag ccattcctaa ttattttcct 240 aattttgatg ctgtaatgaa ataaacaatc gtcagtgaga tcaccgagta tcacgaaaag 300 gaggccagca gcgaaggtcc ttgccccgca ggcgcgaaca ggaaaatcac accctaccgc 360 gcctggctga ggcgaaggga ggccatttct ccctgctcct gcagggcagg gtgtccaggc 420 tccagcacct ccagaggccg agcaggagag ccgcgtgcgg cgcaggtcct gttcggcgag 480 cccgatccgc ccgacccagc atgtctacct acccgccgat cc 522 // ID SCPV02062848; SV 1; linear; genomic DNA; STD; VRT; 565 BP. XX AC SCPV02062848; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22431.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-565 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-565 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4fc704cdfe0dd0977158e88074bd00eb. DR ENA-CON; ML191248. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..565 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22431.1" XX SQ Sequence 565 BP; 182 A; 105 C; 134 G; 144 T; 0 other; ggaacagaat gctgatgatt caacgctatg tggaagtcag tcctgccaca gagagacagt 60 gggtagctgc tggaggtcat atcactttta agcaaagtat ggggccttct ggacaagccc 120 accctcctcc acagacactt ccaaggtcaa aatcgcccag tgggcagaaa aggtcaagat 180 caagatcacc acatgaggct ggcttttgtg tttacttaaa agggctacca tttgaagcag 240 aaaacaaaca tgtcattgat ttttttaaga agttggatat tgtggaagat agtatttata 300 tagcttatgg acccaatggg aaagcaactg gtgaaggctt tgtagaattt aggaatgatg 360 ctgactataa ggctgctctc tgtcgtcata aacaatacat gggcaatcgc tttattcaag 420 ttcatccaat aactaagaaa ggtatgcttg aaaagataga tatgattcga aaacgacttc 480 agaacttcag ctatgaccag agggaactgg tgttaaatcc agagggggaa gtcagttctg 540 ccaaagtctg tgcccatata agaaa 565 // ID SCPV02062849; SV 1; linear; genomic DNA; STD; VRT; 565 BP. XX AC SCPV02062849; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22431.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-565 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-565 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4fc704cdfe0dd0977158e88074bd00eb. DR ENA-CON; ML191248. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..565 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22431.2" XX SQ Sequence 565 BP; 182 A; 105 C; 134 G; 144 T; 0 other; ggaacagaat gctgatgatt caacgctatg tggaagtcag tcctgccaca gagagacagt 60 gggtagctgc tggaggtcat atcactttta agcaaagtat ggggccttct ggacaagccc 120 accctcctcc acagacactt ccaaggtcaa aatcgcccag tgggcagaaa aggtcaagat 180 caagatcacc acatgaggct ggcttttgtg tttacttaaa agggctacca tttgaagcag 240 aaaacaaaca tgtcattgat ttttttaaga agttggatat tgtggaagat agtatttata 300 tagcttatgg acccaatggg aaagcaactg gtgaaggctt tgtagaattt aggaatgatg 360 ctgactataa ggctgctctc tgtcgtcata aacaatacat gggcaatcgc tttattcaag 420 ttcatccaat aactaagaaa ggtatgcttg aaaagataga tatgattcga aaacgacttc 480 agaacttcag ctatgaccag agggaactgg tgttaaatcc agagggggaa gtcagttctg 540 ccaaagtctg tgcccatata agaaa 565 // ID SCPV02062850; SV 1; linear; genomic DNA; STD; VRT; 615 BP. XX AC SCPV02062850; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22432.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-615 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-615 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f3686dffc0f5062479c56a6653dd4223. DR ENA-CON; ML191249. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..615 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22432.1" XX SQ Sequence 615 BP; 204 A; 101 C; 111 G; 199 T; 0 other; ttttttttaa ctgtaagggt ttggataagg ggaataagag aggatagcga ggagatggca 60 gtgataaggg tttttagtgc agacaaacag agggataaaa gatcaacagc ggaaaggaga 120 cttctgaaat tttctgcatt ttttaaaatg gatgttgttg tgccatggca acaaaaagca 180 gcattgcaat tatatgtgca gtcacttcca aatgcagttt aagatgggtt cctcttcttc 240 ctccctttca tttatataaa gtcagatgga atcattctgc actgagaagt gttagccaaa 300 tttcaccaaa aaaaaaaaat taaaaaaaaa acaataatcc caaattattt ctcagtagtt 360 ttcggtttgt tcttgaaact ttcctcctag actgcagggg gtgctcacac tcttttttct 420 gtgcaaaagc aattcctctg cctctagcat ttatttcatt tcacttttat tttattgttt 480 taaatccagg atttcctaat cccaccagta ttatatttta aaaagaaaaa tataaaatga 540 tcaaaagttt ttcctgtgat tttagcaggc ctaggcctag gctggatcat acactcaaaa 600 ggaaatttaa aaaaa 615 // ID SCPV02062851; SV 1; linear; genomic DNA; STD; VRT; 615 BP. XX AC SCPV02062851; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22432.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-615 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-615 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f3686dffc0f5062479c56a6653dd4223. DR ENA-CON; ML191249. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..615 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22432.2" XX SQ Sequence 615 BP; 204 A; 101 C; 111 G; 199 T; 0 other; ttttttttaa ctgtaagggt ttggataagg ggaataagag aggatagcga ggagatggca 60 gtgataaggg tttttagtgc agacaaacag agggataaaa gatcaacagc ggaaaggaga 120 cttctgaaat tttctgcatt ttttaaaatg gatgttgttg tgccatggca acaaaaagca 180 gcattgcaat tatatgtgca gtcacttcca aatgcagttt aagatgggtt cctcttcttc 240 ctccctttca tttatataaa gtcagatgga atcattctgc actgagaagt gttagccaaa 300 tttcaccaaa aaaaaaaaat taaaaaaaaa acaataatcc caaattattt ctcagtagtt 360 ttcggtttgt tcttgaaact ttcctcctag actgcagggg gtgctcacac tcttttttct 420 gtgcaaaagc aattcctctg cctctagcat ttatttcatt tcacttttat tttattgttt 480 taaatccagg atttcctaat cccaccagta ttatatttta aaaagaaaaa tataaaatga 540 tcaaaagttt ttcctgtgat tttagcaggc ctaggcctag gctggatcat acactcaaaa 600 ggaaatttaa aaaaa 615 // ID SCPV02062852; SV 1; linear; genomic DNA; STD; VRT; 695 BP. XX AC SCPV02062852; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22433.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-695 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-695 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 38d3f7561a83a550a637a960cee7af6f. DR ENA-CON; ML191250. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..695 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22433.1" XX SQ Sequence 695 BP; 260 A; 171 C; 111 G; 153 T; 0 other; aaaaaacaaa taaatcccta agccacatag taatggaata ctacacttgc tttttatatc 60 atatggaaac agctccaggc agagcacaag tagaagaaaa cctgccaaat caaagtactg 120 taaataaagt tcctctacgt actccagttg acaaaacctc aaaaataaaa atcagaagac 180 cacccattgt taccctcccc cccgcaacaa aacccaccca aaacacttta gttctgaacc 240 taacatttcc ctaagaagtt cacaggagaa gctttaaggt acttaaagct gcagcagtac 300 agttgaaagc gccacattac agaggaaatg ccctaaaggg ttcagcagct gcccacccat 360 cccgaaccac cccccatccc agtaatatgt aaaagatcct gttaaagttt tttttgtttt 420 atgctaataa aggcatacca cctagtgtgc taaagactac gactagcagc attagagcag 480 taaagtgaaa ataggaagcc tacaggaatg atctaaatgc ctacttagaa gaaacaaagc 540 agcagacaga atccttagcc ctctagtcta tgtagggaag cagttgaatt aaactcagat 600 ttacccacat taacaaaaat ttaacaaacc ttccccaaga caccagaata aaacttgacc 660 tttccagtca aagggcagag agaggcctct tccat 695 // ID SCPV02062853; SV 1; linear; genomic DNA; STD; VRT; 695 BP. XX AC SCPV02062853; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22433.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-695 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-695 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 38d3f7561a83a550a637a960cee7af6f. DR ENA-CON; ML191250. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..695 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22433.2" XX SQ Sequence 695 BP; 260 A; 171 C; 111 G; 153 T; 0 other; aaaaaacaaa taaatcccta agccacatag taatggaata ctacacttgc tttttatatc 60 atatggaaac agctccaggc agagcacaag tagaagaaaa cctgccaaat caaagtactg 120 taaataaagt tcctctacgt actccagttg acaaaacctc aaaaataaaa atcagaagac 180 cacccattgt taccctcccc cccgcaacaa aacccaccca aaacacttta gttctgaacc 240 taacatttcc ctaagaagtt cacaggagaa gctttaaggt acttaaagct gcagcagtac 300 agttgaaagc gccacattac agaggaaatg ccctaaaggg ttcagcagct gcccacccat 360 cccgaaccac cccccatccc agtaatatgt aaaagatcct gttaaagttt tttttgtttt 420 atgctaataa aggcatacca cctagtgtgc taaagactac gactagcagc attagagcag 480 taaagtgaaa ataggaagcc tacaggaatg atctaaatgc ctacttagaa gaaacaaagc 540 agcagacaga atccttagcc ctctagtcta tgtagggaag cagttgaatt aaactcagat 600 ttacccacat taacaaaaat ttaacaaacc ttccccaaga caccagaata aaacttgacc 660 tttccagtca aagggcagag agaggcctct tccat 695 // ID SCPV02062854; SV 1; linear; genomic DNA; STD; VRT; 1008 BP. XX AC SCPV02062854; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22434.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-1008 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-1008 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; abb8b7928aa5b0ae4eb1de1f1b171188. DR ENA-CON; ML191251. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1008 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22434.1" XX SQ Sequence 1008 BP; 341 A; 184 C; 143 G; 340 T; 0 other; tctaactaca gtgctttgtg tcactgtaaa taaaggtgac atactgccaa ggggagcaag 60 aaaaggaaaa acagctgatt gctattatta aaaggattgt tctccattgt aatatgtgtt 120 gtaaaacagt attatttcca atgttagtga acaaataact ttctagattg ttttgtcaca 180 ggtttatttt caaagtgtta tgtcatttca tggttttaat catctagcta cattttccat 240 aacctgctta caaagaagtc atcaacttct tcttctcttc actaatggat ttagaaaaca 300 cctgtaagta tccttttttt ctatacatag taattctaca agtttcacac aaattccagt 360 tgcaaaatga tttcaaatca ccttttactc tcaatgatct tatttttaaa atacattccg 420 tcagaagtaa catgaactat aaaccataat tctaaagaga agcataaaga aataaccgga 480 taggtaacct tcattacaaa agtcacatgc attatttatg accacataat tgagtgccaa 540 aagtgagtct tgagtgttgc atggggccac atgacttatg acaaatcatc aatctcagtc 600 tactgttgct tgggatattg gtgataaatg cagtcatctt atgatcctag taagtatgcc 660 agacttttta attaaactaa gccacaaggg gttaagaatc acatcttaat cttctccctc 720 tttttttttt aaatcatcat gtctttctaa agatctagaa aataaaagtt agaatcttgc 780 tttttatgct tttccttccg atcatacaca taatgtatat tgcccaggga taaaaatcac 840 aaaaaataaa atttaaaaaa cccacacaga tatacaatat tttgtatcta aatactctct 900 tccaagcaag gatctcaaag cactttttga gtcactcttt gtgactggca gaatgtttct 960 ctcctaagct ccaggtataa cataggttga aatattcaca gccaacta 1008 // ID SCPV02062855; SV 1; linear; genomic DNA; STD; VRT; 1008 BP. XX AC SCPV02062855; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22434.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-1008 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-1008 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; abb8b7928aa5b0ae4eb1de1f1b171188. DR ENA-CON; ML191251. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1008 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22434.2" XX SQ Sequence 1008 BP; 341 A; 184 C; 143 G; 340 T; 0 other; tctaactaca gtgctttgtg tcactgtaaa taaaggtgac atactgccaa ggggagcaag 60 aaaaggaaaa acagctgatt gctattatta aaaggattgt tctccattgt aatatgtgtt 120 gtaaaacagt attatttcca atgttagtga acaaataact ttctagattg ttttgtcaca 180 ggtttatttt caaagtgtta tgtcatttca tggttttaat catctagcta cattttccat 240 aacctgctta caaagaagtc atcaacttct tcttctcttc actaatggat ttagaaaaca 300 cctgtaagta tccttttttt ctatacatag taattctaca agtttcacac aaattccagt 360 tgcaaaatga tttcaaatca ccttttactc tcaatgatct tatttttaaa atacattccg 420 tcagaagtaa catgaactat aaaccataat tctaaagaga agcataaaga aataaccgga 480 taggtaacct tcattacaaa agtcacatgc attatttatg accacataat tgagtgccaa 540 aagtgagtct tgagtgttgc atggggccac atgacttatg acaaatcatc aatctcagtc 600 tactgttgct tgggatattg gtgataaatg cagtcatctt atgatcctag taagtatgcc 660 agacttttta attaaactaa gccacaaggg gttaagaatc acatcttaat cttctccctc 720 tttttttttt aaatcatcat gtctttctaa agatctagaa aataaaagtt agaatcttgc 780 tttttatgct tttccttccg atcatacaca taatgtatat tgcccaggga taaaaatcac 840 aaaaaataaa atttaaaaaa cccacacaga tatacaatat tttgtatcta aatactctct 900 tccaagcaag gatctcaaag cactttttga gtcactcttt gtgactggca gaatgtttct 960 ctcctaagct ccaggtataa cataggttga aatattcaca gccaacta 1008 // ID SCPV02062856; SV 1; linear; genomic DNA; STD; VRT; 735 BP. XX AC SCPV02062856; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22435.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-735 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-735 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 0d53aca32cd022c828c9891bc4c5d51e. DR ENA-CON; ML191252. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..735 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22435.1" XX SQ Sequence 735 BP; 196 A; 233 C; 176 G; 130 T; 0 other; gcgacgacat catcggctcc caggagtagc tccacaccta ccaccacgaa tagcagctcc 60 tttgggctgg gaagcctttc cagccttagt aacctgggct tgaactcgcc caacttcacc 120 gagcttcaga accagatgca gcagcagctc ctggccagcc ctgagatgat gatccagatc 180 atggaaaatc cctttgttca gagcatgctt tcgaatcctg atctgatgag gcagctcatc 240 atggccaatc cacagatgca acaattgatc cagagaaacc cagaaatcag ccacctactg 300 aacaacccag atataatgag gcagacgctg gaaatcgcca ggaatcctgc catgatgcaa 360 gagatgatgc gaaatcaaga cctggctctc agtaatctcg aaagcatccc aggtggctac 420 aatgctctgc ggcgcatgta cactgacatt caagaaccta tgctgaatgc cgcacaggag 480 cagtttgggg gcaacccgtt tgccacggtg gggagcagtt ccacctcagg ggaaggtact 540 cagccctccc gcacggagaa tcgtgatccg ctgcccaatc cttgggcacc accgccaaca 600 acccagacgg ctgcgaccac caccaccacc acgaccacaa gcagtggcag tggctctggc 660 agcagctcca gcagcaccac cgccgggaac accatggctg cagctaatta cgtggctagc 720 atcttcagca cccca 735 // ID SCPV02062857; SV 1; linear; genomic DNA; STD; VRT; 735 BP. XX AC SCPV02062857; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22435.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-735 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-735 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 0d53aca32cd022c828c9891bc4c5d51e. DR ENA-CON; ML191252. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..735 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22435.2" XX SQ Sequence 735 BP; 196 A; 233 C; 176 G; 130 T; 0 other; gcgacgacat catcggctcc caggagtagc tccacaccta ccaccacgaa tagcagctcc 60 tttgggctgg gaagcctttc cagccttagt aacctgggct tgaactcgcc caacttcacc 120 gagcttcaga accagatgca gcagcagctc ctggccagcc ctgagatgat gatccagatc 180 atggaaaatc cctttgttca gagcatgctt tcgaatcctg atctgatgag gcagctcatc 240 atggccaatc cacagatgca acaattgatc cagagaaacc cagaaatcag ccacctactg 300 aacaacccag atataatgag gcagacgctg gaaatcgcca ggaatcctgc catgatgcaa 360 gagatgatgc gaaatcaaga cctggctctc agtaatctcg aaagcatccc aggtggctac 420 aatgctctgc ggcgcatgta cactgacatt caagaaccta tgctgaatgc cgcacaggag 480 cagtttgggg gcaacccgtt tgccacggtg gggagcagtt ccacctcagg ggaaggtact 540 cagccctccc gcacggagaa tcgtgatccg ctgcccaatc cttgggcacc accgccaaca 600 acccagacgg ctgcgaccac caccaccacc acgaccacaa gcagtggcag tggctctggc 660 agcagctcca gcagcaccac cgccgggaac accatggctg cagctaatta cgtggctagc 720 atcttcagca cccca 735 // ID SCPV02062858; SV 1; linear; genomic DNA; STD; VRT; 600 BP. XX AC SCPV02062858; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22436.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-600 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-600 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; a7dd95e3b61d0fed110f871ca89007d9. DR ENA-CON; ML191253. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..600 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22436.1" XX SQ Sequence 600 BP; 218 A; 125 C; 109 G; 148 T; 0 other; agatgcacgg cgctgacgag ccatactact caaaacatag accgcagaag agtccatagg 60 tgtgaagtca tagcttttaa atgtatcaaa aacacctgaa tcatcgaaat taatggcatc 120 agggtgtcca ggaggtagac atacgtcacc aatatgaatt tcacaacatg gaatgccatg 180 ctgtaccaca gtcaagcttg gataaaatga cgaccaacct ttatttttaa catagaaagg 240 gtggtccagc ttgcactcta cagtgagcaa gccatcttct actgtaccag gatcaaatgt 300 caacttcagt acagactcac cgaatgacac actccctata tgggataaca actttagacc 360 atcagaccca tagccctgaa agcatatgca tggactaaat aaattggaaa cattccattc 420 aacagggaaa ctcccaatat atgtctggat tggaaaaggt aaatttagtc agtatctcca 480 gctttaaagt agtttcaacc aagcagtaaa gaaacacacg tatcatgacc acagaatatg 540 tatcagattg agataatatg cattttaatc caaatataag taatatgaag tttagtaaac 600 // ID SCPV02062859; SV 1; linear; genomic DNA; STD; VRT; 600 BP. XX AC SCPV02062859; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22436.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-600 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-600 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; a7dd95e3b61d0fed110f871ca89007d9. DR ENA-CON; ML191253. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..600 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22436.2" XX SQ Sequence 600 BP; 218 A; 125 C; 109 G; 148 T; 0 other; agatgcacgg cgctgacgag ccatactact caaaacatag accgcagaag agtccatagg 60 tgtgaagtca tagcttttaa atgtatcaaa aacacctgaa tcatcgaaat taatggcatc 120 agggtgtcca ggaggtagac atacgtcacc aatatgaatt tcacaacatg gaatgccatg 180 ctgtaccaca gtcaagcttg gataaaatga cgaccaacct ttatttttaa catagaaagg 240 gtggtccagc ttgcactcta cagtgagcaa gccatcttct actgtaccag gatcaaatgt 300 caacttcagt acagactcac cgaatgacac actccctata tgggataaca actttagacc 360 atcagaccca tagccctgaa agcatatgca tggactaaat aaattggaaa cattccattc 420 aacagggaaa ctcccaatat atgtctggat tggaaaaggt aaatttagtc agtatctcca 480 gctttaaagt agtttcaacc aagcagtaaa gaaacacacg tatcatgacc acagaatatg 540 tatcagattg agataatatg cattttaatc caaatataag taatatgaag tttagtaaac 600 // ID SCPV02062860; SV 1; linear; genomic DNA; STD; VRT; 701 BP. XX AC SCPV02062860; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22437.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-701 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-701 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4210c2c58864484a6a22f4addb998f8e. DR ENA-CON; ML191254. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..701 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22437.1" XX SQ Sequence 701 BP; 174 A; 148 C; 157 G; 222 T; 0 other; aatggaaagg ccatttgaca tgtgttgttg tcttaggagt ggtgatattg tagctccaag 60 catcagcctg ctgtttaggt cctagtatat ttagttcagg agatttcatt tggggtttga 120 cctggttttt actgtacctc tcctaatcac agaatcctgg cctagcaatg ccaggcagga 180 gaaatatcct ctgaaagcaa cctgcaggca gctcggctat tctcactgcc attagcatga 240 gcagcgctgt ctccgtggtg attccagaac acaggagttt agcgctatgc tgatactacg 300 ctgtctttac cagtaagatt tgatcatatg atcctttatt tggatgtacg tatttttatg 360 ctgcctgttt cagtggacat aattgccacc caacatttcc aatatggtgg ccagctttac 420 atttcccacc caggtttcac catttgctct agacctgggc caatggaaga cattagaacg 480 acacgaggtg gtgtcagagg tgactaatgc tgggaattag aggagtttgg gatgttttgt 540 tttgggaatg agttggtaag aatagggata gccccagaat gaaatgccaa aacaccagtt 600 attcaaacac agttttgtta ctcagtatga tgtaatattt taggtacatt ttccccatgc 660 tttccatcca cattcagttt ccattcttgc ccattgttcc c 701 // ID SCPV02062861; SV 1; linear; genomic DNA; STD; VRT; 701 BP. XX AC SCPV02062861; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22437.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-701 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-701 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4210c2c58864484a6a22f4addb998f8e. DR ENA-CON; ML191254. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..701 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22437.2" XX SQ Sequence 701 BP; 174 A; 148 C; 157 G; 222 T; 0 other; aatggaaagg ccatttgaca tgtgttgttg tcttaggagt ggtgatattg tagctccaag 60 catcagcctg ctgtttaggt cctagtatat ttagttcagg agatttcatt tggggtttga 120 cctggttttt actgtacctc tcctaatcac agaatcctgg cctagcaatg ccaggcagga 180 gaaatatcct ctgaaagcaa cctgcaggca gctcggctat tctcactgcc attagcatga 240 gcagcgctgt ctccgtggtg attccagaac acaggagttt agcgctatgc tgatactacg 300 ctgtctttac cagtaagatt tgatcatatg atcctttatt tggatgtacg tatttttatg 360 ctgcctgttt cagtggacat aattgccacc caacatttcc aatatggtgg ccagctttac 420 atttcccacc caggtttcac catttgctct agacctgggc caatggaaga cattagaacg 480 acacgaggtg gtgtcagagg tgactaatgc tgggaattag aggagtttgg gatgttttgt 540 tttgggaatg agttggtaag aatagggata gccccagaat gaaatgccaa aacaccagtt 600 attcaaacac agttttgtta ctcagtatga tgtaatattt taggtacatt ttccccatgc 660 tttccatcca cattcagttt ccattcttgc ccattgttcc c 701 // ID SCPV02062862; SV 1; linear; genomic DNA; STD; VRT; 577 BP. XX AC SCPV02062862; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22438.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-577 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-577 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; a882f9a49b5087804fddf1cb30d5148e. DR ENA-CON; ML191255. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..577 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22438.1" XX SQ Sequence 577 BP; 85 A; 164 C; 184 G; 144 T; 0 other; gcgcctcctc aaccgccatg tttacattgg ccgcgtctgg gcacttccgt tgtcgtggac 60 agtagggtct tttgtatggt ttttcttttt ttctttcttt ttttttcgag actacctcta 120 cgatcaactg cgctactttt gacaatgaaa gtcctgggga ctcggtggga gcggcgactg 180 gggcgcgagg gcggggtctt gcccggacta aagatggccg ccgccagtgc cgccccgcgc 240 aatttcacgt caggttctgg agcccaggga gccgcggggt tggccccccg ctccttacgg 300 aattggcgta aagctgggag gcgccggtta aagagcctgg cagctcttgg aaaactgccc 360 tcggggtggg ggccccgcag aggtcgctgg gccgtggacc tgacgtcttt gaaagaagaa 420 ggcaggtgtc tgtcctcgtc tcctcactcg ggtgactttg tgggtgggta gcgaggttag 480 gccctgctcg cctggacact tcctgctggt catccccggt ggtctggcgc acacatgagg 540 ccggctgtgc agtctcctca cactcccttg gtgactc 577 // ID SCPV02062863; SV 1; linear; genomic DNA; STD; VRT; 577 BP. XX AC SCPV02062863; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22438.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-577 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-577 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; a882f9a49b5087804fddf1cb30d5148e. DR ENA-CON; ML191255. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..577 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22438.2" XX SQ Sequence 577 BP; 85 A; 164 C; 184 G; 144 T; 0 other; gcgcctcctc aaccgccatg tttacattgg ccgcgtctgg gcacttccgt tgtcgtggac 60 agtagggtct tttgtatggt ttttcttttt ttctttcttt ttttttcgag actacctcta 120 cgatcaactg cgctactttt gacaatgaaa gtcctgggga ctcggtggga gcggcgactg 180 gggcgcgagg gcggggtctt gcccggacta aagatggccg ccgccagtgc cgccccgcgc 240 aatttcacgt caggttctgg agcccaggga gccgcggggt tggccccccg ctccttacgg 300 aattggcgta aagctgggag gcgccggtta aagagcctgg cagctcttgg aaaactgccc 360 tcggggtggg ggccccgcag aggtcgctgg gccgtggacc tgacgtcttt gaaagaagaa 420 ggcaggtgtc tgtcctcgtc tcctcactcg ggtgactttg tgggtgggta gcgaggttag 480 gccctgctcg cctggacact tcctgctggt catccccggt ggtctggcgc acacatgagg 540 ccggctgtgc agtctcctca cactcccttg gtgactc 577 // ID SCPV02062864; SV 1; linear; genomic DNA; STD; VRT; 585 BP. XX AC SCPV02062864; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22439.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-585 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-585 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f3877bb4e9f9cf05d8de81acf728f2cb. DR ENA-CON; ML191256. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..585 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22439.1" XX SQ Sequence 585 BP; 97 A; 205 C; 187 G; 96 T; 0 other; cgggagccaa ccgagggcgt tcctgtcggg gctgcagcgg cgggaggtaa ggcatggcca 60 ggccggctgg gctgcagagc gccggcacgg gtccacgcct cgggtgacgg gcttccagga 120 tgttcgggcg cggggcggcc catccgcatc ccccaacacc cccacctccg gcctgagcct 180 cccagcgccg tgggaaccac ctcctgtccg ctgttgctgg cccgcatcct agcagcggcc 240 tgacgccctc cccaccctgg catgccccct tgacctggga cgatgagcat acgactgggg 300 agcccagtgg aggcgccctc ccgaagcgcc actgcccatg ctgaccaccc agccctccgg 360 ctgctgatgt catgagtaac accactgtgc ccaatgcccc ccaggccaac agcgactcca 420 tggtgggcta tgtgttgggg cccttcttcc tcatcaccct ggtcggggtg gtggtggctg 480 tggtaatgta tgtacagaag aaaaagcggg tggaccggct gcgccatcac ctgctcccca 540 tgtacagcta tgacccagct gaggaactgc atgaggctga gcagg 585 // ID SCPV02062865; SV 1; linear; genomic DNA; STD; VRT; 585 BP. XX AC SCPV02062865; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22439.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-585 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-585 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f3877bb4e9f9cf05d8de81acf728f2cb. DR ENA-CON; ML191256. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..585 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22439.2" XX SQ Sequence 585 BP; 97 A; 205 C; 187 G; 96 T; 0 other; cgggagccaa ccgagggcgt tcctgtcggg gctgcagcgg cgggaggtaa ggcatggcca 60 ggccggctgg gctgcagagc gccggcacgg gtccacgcct cgggtgacgg gcttccagga 120 tgttcgggcg cggggcggcc catccgcatc ccccaacacc cccacctccg gcctgagcct 180 cccagcgccg tgggaaccac ctcctgtccg ctgttgctgg cccgcatcct agcagcggcc 240 tgacgccctc cccaccctgg catgccccct tgacctggga cgatgagcat acgactgggg 300 agcccagtgg aggcgccctc ccgaagcgcc actgcccatg ctgaccaccc agccctccgg 360 ctgctgatgt catgagtaac accactgtgc ccaatgcccc ccaggccaac agcgactcca 420 tggtgggcta tgtgttgggg cccttcttcc tcatcaccct ggtcggggtg gtggtggctg 480 tggtaatgta tgtacagaag aaaaagcggg tggaccggct gcgccatcac ctgctcccca 540 tgtacagcta tgacccagct gaggaactgc atgaggctga gcagg 585 // ID SCPV02062866; SV 1; linear; genomic DNA; STD; VRT; 638 BP. XX AC SCPV02062866; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22440.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-638 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-638 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e5bae72ae2969a33fe4ab8743ee78428. DR ENA-CON; ML191257. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..638 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22440.1" XX SQ Sequence 638 BP; 187 A; 177 C; 149 G; 125 T; 0 other; ctttccacgc caccccaaat gtatataagc gcaagaacaa ggaaatcaag attgagccag 60 aaccgtgtgg cacagactgc ttccttttgc tggaaggagc aaaggagtac gccatgctgc 120 acaaccctcg gtccaagtgc tctgggcgcc gccgccgaag gcacccagtg gtcagtgctt 180 cctgctccaa tgcatcagct tctgctatgg ctgaaactaa agaaggagac agtgatagag 240 acactggcaa tgactgggcc tccagttctt cagaggctaa ctctcgctgt cagaccccca 300 cgaaacagaa agccagtcca gccccagctc agctctgtgt tgtggaagcc ccctcagagc 360 cggtggaatg gaccggagcc gaagaatctc ttttccgagt cttccacggc acctatttca 420 acaacttctg ctcaatagcc aggcttctgg ggacaaagac atgcaagcag gtctttcagt 480 ttgcagtcaa agaatcactt atcctaaagc tgccaacaga tgagctcatg aaccctgcac 540 agaagaagaa aagaaaacac aggttgtggg ccgcacactg caggaaaatt cagctgaaga 600 aagataacaa ttctacacaa gtgtataact accaaccc 638 // ID SCPV02062867; SV 1; linear; genomic DNA; STD; VRT; 638 BP. XX AC SCPV02062867; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22440.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-638 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-638 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; e5bae72ae2969a33fe4ab8743ee78428. DR ENA-CON; ML191257. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..638 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22440.2" XX SQ Sequence 638 BP; 187 A; 177 C; 149 G; 125 T; 0 other; ctttccacgc caccccaaat gtatataagc gcaagaacaa ggaaatcaag attgagccag 60 aaccgtgtgg cacagactgc ttccttttgc tggaaggagc aaaggagtac gccatgctgc 120 acaaccctcg gtccaagtgc tctgggcgcc gccgccgaag gcacccagtg gtcagtgctt 180 cctgctccaa tgcatcagct tctgctatgg ctgaaactaa agaaggagac agtgatagag 240 acactggcaa tgactgggcc tccagttctt cagaggctaa ctctcgctgt cagaccccca 300 cgaaacagaa agccagtcca gccccagctc agctctgtgt tgtggaagcc ccctcagagc 360 cggtggaatg gaccggagcc gaagaatctc ttttccgagt cttccacggc acctatttca 420 acaacttctg ctcaatagcc aggcttctgg ggacaaagac atgcaagcag gtctttcagt 480 ttgcagtcaa agaatcactt atcctaaagc tgccaacaga tgagctcatg aaccctgcac 540 agaagaagaa aagaaaacac aggttgtggg ccgcacactg caggaaaatt cagctgaaga 600 aagataacaa ttctacacaa gtgtataact accaaccc 638 // ID SCPV02062868; SV 1; linear; genomic DNA; STD; VRT; 883 BP. XX AC SCPV02062868; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22441.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-883 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-883 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 3d450a35dda36e0ad8529a8804ce21a2. DR ENA-CON; ML191258. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..883 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22441.1" XX SQ Sequence 883 BP; 200 A; 236 C; 218 G; 229 T; 0 other; gccagacacc ttgattttgc tgagatccag gcttggagat actcccactg aaaatggact 60 cttggggatg tgatctcctc cataagtgac attgacacct actgggccct gctgcacagg 120 aatgtacttg actgtgtagg tattatcatg gtggtcaatg atgtccacat cccgtactgc 180 atctccctta gccagtcctg agaattggac atccagcttg cctttcccag cagttttagc 240 attgactgtg aaatgggtgg gtttgccaag ttcaacacca gtgcgattta ggccaggacc 300 ctcagccttc accttgctgg catcatgaga aggctccact ttgactctga tggggctggt 360 gggtgtggcc tggtcagcaa aaaggaccat gatggtatag ctgccagccc cacagggtgt 420 gtattttaca gtgaaggtgt cattgtcatt gcggatgata tcaaagtcaa tatcagcctc 480 agtggggccc actactccag gggcacactt gataccaatg ctgacatctc cctggccagc 540 ttcagtacaa tccacagtaa agtaggtagg ttcatgggcc ttgagcccag tcttggccac 600 tcctggacca tacaccttga ctttgtttgg atggctgcca gctcccacat tcacccggaa 660 aggactgttg gggatgctga cacctcccca agaaaccatg gctgtgtgct tcactggctt 720 tctgggcaca taagaacagc tgtaagtacc attgccattg tccttgactg tcgcttccac 780 agagcagccc tcattgtcct gaacttgaac tcgaagagga gccttcccag catgcttggc 840 atcaactgtg aactctgctg gcttgttgac agccacacca gtc 883 // ID SCPV02062869; SV 1; linear; genomic DNA; STD; VRT; 883 BP. XX AC SCPV02062869; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22441.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-883 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-883 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 3d450a35dda36e0ad8529a8804ce21a2. DR ENA-CON; ML191258. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..883 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22441.2" XX SQ Sequence 883 BP; 200 A; 236 C; 218 G; 229 T; 0 other; gccagacacc ttgattttgc tgagatccag gcttggagat actcccactg aaaatggact 60 cttggggatg tgatctcctc cataagtgac attgacacct actgggccct gctgcacagg 120 aatgtacttg actgtgtagg tattatcatg gtggtcaatg atgtccacat cccgtactgc 180 atctccctta gccagtcctg agaattggac atccagcttg cctttcccag cagttttagc 240 attgactgtg aaatgggtgg gtttgccaag ttcaacacca gtgcgattta ggccaggacc 300 ctcagccttc accttgctgg catcatgaga aggctccact ttgactctga tggggctggt 360 gggtgtggcc tggtcagcaa aaaggaccat gatggtatag ctgccagccc cacagggtgt 420 gtattttaca gtgaaggtgt cattgtcatt gcggatgata tcaaagtcaa tatcagcctc 480 agtggggccc actactccag gggcacactt gataccaatg ctgacatctc cctggccagc 540 ttcagtacaa tccacagtaa agtaggtagg ttcatgggcc ttgagcccag tcttggccac 600 tcctggacca tacaccttga ctttgtttgg atggctgcca gctcccacat tcacccggaa 660 aggactgttg gggatgctga cacctcccca agaaaccatg gctgtgtgct tcactggctt 720 tctgggcaca taagaacagc tgtaagtacc attgccattg tccttgactg tcgcttccac 780 agagcagccc tcattgtcct gaacttgaac tcgaagagga gccttcccag catgcttggc 840 atcaactgtg aactctgctg gcttgttgac agccacacca gtc 883 // ID SCPV02062870; SV 1; linear; genomic DNA; STD; VRT; 501 BP. XX AC SCPV02062870; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22442.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-501 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-501 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 626fe82777e1dd8fdc38360f7f88863c. DR ENA-CON; ML191259. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..501 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22442.1" XX SQ Sequence 501 BP; 95 A; 133 C; 90 G; 183 T; 0 other; gtgaatactc ttctgcatct tctccacctc atgtgcggta gcagcctgct cattagccag 60 ggcctgcaat ttctgcactt cagcttttaa taagaaattc atattctgta catccttaag 120 ctcctcctcc ttgcttgcta agtgatcctg ttcattcgct aatgaatctt cagtctgctt 180 tagctgcttg tccttttctg caatcctgag ctgctatctg ggaatggaat tgttgaatct 240 gagctttcaa agcctcgttt tgttccaaaa tatcctgcac ttggatctgc agagactctt 300 ctttgctcgc cctcttttgc tcggattcca tcaactgcat tagtctttgc tccaattgct 360 gttttgatac caaggtgtct gtgagcttac tatgtagact ctggacttca ttttctttgg 420 ccacaaattt actttgtaaa tcttgctggg cagcctcatg ctccgccgtc ctcttcctga 480 tgtagctctg aacttcttcc c 501 // ID SCPV02062871; SV 1; linear; genomic DNA; STD; VRT; 501 BP. XX AC SCPV02062871; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22442.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-501 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-501 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 626fe82777e1dd8fdc38360f7f88863c. DR ENA-CON; ML191259. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..501 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22442.2" XX SQ Sequence 501 BP; 95 A; 133 C; 90 G; 183 T; 0 other; gtgaatactc ttctgcatct tctccacctc atgtgcggta gcagcctgct cattagccag 60 ggcctgcaat ttctgcactt cagcttttaa taagaaattc atattctgta catccttaag 120 ctcctcctcc ttgcttgcta agtgatcctg ttcattcgct aatgaatctt cagtctgctt 180 tagctgcttg tccttttctg caatcctgag ctgctatctg ggaatggaat tgttgaatct 240 gagctttcaa agcctcgttt tgttccaaaa tatcctgcac ttggatctgc agagactctt 300 ctttgctcgc cctcttttgc tcggattcca tcaactgcat tagtctttgc tccaattgct 360 gttttgatac caaggtgtct gtgagcttac tatgtagact ctggacttca ttttctttgg 420 ccacaaattt actttgtaaa tcttgctggg cagcctcatg ctccgccgtc ctcttcctga 480 tgtagctctg aacttcttcc c 501 // ID SCPV02062872; SV 1; linear; genomic DNA; STD; VRT; 653 BP. XX AC SCPV02062872; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22443.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-653 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-653 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; acb9da014b92be20c6552618058d2d2f. DR ENA-CON; ML191260. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..653 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22443.1" XX SQ Sequence 653 BP; 215 A; 120 C; 195 G; 123 T; 0 other; tctgtcaagc tcaacgggac tactcacatg cataaagtta agcagaagtg tttgcagggt 60 cagccaccta gagttggcct ttggttccat acgccataaa gcccagcacc aacagtcctc 120 ttgtttgggg gcaagaagaa ctggatctat ttatagtagg gatacctagg ctatccagat 180 actgataggt tatgacctcc atcattatag tgtctgagtg ccctccgcat acttaaaaag 240 acaaacaaca gggctgccat ctgtatcact agactctgtg actgcagaag tggcagcaca 300 gtggagatcg cttaatacag gcagctgaca taatgggaat tagaccttgc tctttgtgca 360 caccccttca acagcagcaa cagccccctt gggtgggaca agagaaaaca tagggagaag 420 agaaagagga agggagggag ggagacatat cagtggcagg gcatggagat gaaagggaac 480 cacaggaagg agacagtatg aggtggaggg aaatttcaga gtggggagag cgaggagaat 540 ggtctcatgg aaaacagagg gatggagatg agagagagaa ctgaataaga gagagagagc 600 aaaggggcag gggaacagag aatgcaggga gtaaaagctt gacagaatga aat 653 // ID SCPV02062873; SV 1; linear; genomic DNA; STD; VRT; 653 BP. XX AC SCPV02062873; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22443.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-653 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-653 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; acb9da014b92be20c6552618058d2d2f. DR ENA-CON; ML191260. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..653 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22443.2" XX SQ Sequence 653 BP; 215 A; 120 C; 195 G; 123 T; 0 other; tctgtcaagc tcaacgggac tactcacatg cataaagtta agcagaagtg tttgcagggt 60 cagccaccta gagttggcct ttggttccat acgccataaa gcccagcacc aacagtcctc 120 ttgtttgggg gcaagaagaa ctggatctat ttatagtagg gatacctagg ctatccagat 180 actgataggt tatgacctcc atcattatag tgtctgagtg ccctccgcat acttaaaaag 240 acaaacaaca gggctgccat ctgtatcact agactctgtg actgcagaag tggcagcaca 300 gtggagatcg cttaatacag gcagctgaca taatgggaat tagaccttgc tctttgtgca 360 caccccttca acagcagcaa cagccccctt gggtgggaca agagaaaaca tagggagaag 420 agaaagagga agggagggag ggagacatat cagtggcagg gcatggagat gaaagggaac 480 cacaggaagg agacagtatg aggtggaggg aaatttcaga gtggggagag cgaggagaat 540 ggtctcatgg aaaacagagg gatggagatg agagagagaa ctgaataaga gagagagagc 600 aaaggggcag gggaacagag aatgcaggga gtaaaagctt gacagaatga aat 653 // ID SCPV02062874; SV 1; linear; genomic DNA; STD; VRT; 869 BP. XX AC SCPV02062874; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22444.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-869 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-869 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2d16cb41648c76dd1f5d73b7566d9837. DR ENA-CON; ML191261. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..869 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22444.1" XX SQ Sequence 869 BP; 305 A; 152 C; 113 G; 299 T; 0 other; gcatcattta ttcattacct ttcttttgac aacagaattc taaacaaata aaaattaatt 60 tgacctcctc agcacagcct tagttagcaa ttctttacat tagattacaa gagcaataaa 120 atagaagaca catatctggt tcagtttaca ggcctgccca cattgcacct gattcttttc 180 agagacccaa agacaattta tcagattctc agtatatttt ggcagatgta taaaaatcat 240 aaaaaggtta gtgtgttttc attacatcat ggaaatccat ttggagacat ggagtactca 300 atgtatatta tatgggccac cttaaatcaa aggcatcatt tattccattc tcacacacca 360 tcatggtctc tcacacctta tacaggcatg gtcctagacc agagacttca gaatcattcc 420 aaagaataca cagatactta tatacatatt tcttttaaaa taatgtttaa aagttttact 480 acacaaagtc tggcttcaac agagaagctt tttttttttt tccttttcaa gattatacat 540 cagtatggaa aaagttggtt tcctttacat tttgtgtcca caatagtaaa ataaacttct 600 acattgtatt ttaattgaca aaaaattaaa atgtaaagtt gcatttaatt tcttgtacta 660 tgaactaacc ctttaagcac tttcaagata cactgctgat gatttaaatg tagtacaact 720 tgatcagctt aattataact gtgttatgct gaacaagcca ttaaaaatat ggataaaata 780 gtaacaaact agtaagattt aaagggaatt gaatcaataa atatacatca attttttgct 840 attacttgtt tctataacgc atgtctttc 869 // ID SCPV02062875; SV 1; linear; genomic DNA; STD; VRT; 869 BP. XX AC SCPV02062875; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22444.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-869 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-869 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2d16cb41648c76dd1f5d73b7566d9837. DR ENA-CON; ML191261. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..869 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22444.2" XX SQ Sequence 869 BP; 305 A; 152 C; 113 G; 299 T; 0 other; gcatcattta ttcattacct ttcttttgac aacagaattc taaacaaata aaaattaatt 60 tgacctcctc agcacagcct tagttagcaa ttctttacat tagattacaa gagcaataaa 120 atagaagaca catatctggt tcagtttaca ggcctgccca cattgcacct gattcttttc 180 agagacccaa agacaattta tcagattctc agtatatttt ggcagatgta taaaaatcat 240 aaaaaggtta gtgtgttttc attacatcat ggaaatccat ttggagacat ggagtactca 300 atgtatatta tatgggccac cttaaatcaa aggcatcatt tattccattc tcacacacca 360 tcatggtctc tcacacctta tacaggcatg gtcctagacc agagacttca gaatcattcc 420 aaagaataca cagatactta tatacatatt tcttttaaaa taatgtttaa aagttttact 480 acacaaagtc tggcttcaac agagaagctt tttttttttt tccttttcaa gattatacat 540 cagtatggaa aaagttggtt tcctttacat tttgtgtcca caatagtaaa ataaacttct 600 acattgtatt ttaattgaca aaaaattaaa atgtaaagtt gcatttaatt tcttgtacta 660 tgaactaacc ctttaagcac tttcaagata cactgctgat gatttaaatg tagtacaact 720 tgatcagctt aattataact gtgttatgct gaacaagcca ttaaaaatat ggataaaata 780 gtaacaaact agtaagattt aaagggaatt gaatcaataa atatacatca attttttgct 840 attacttgtt tctataacgc atgtctttc 869 // ID SCPV02062876; SV 1; linear; genomic DNA; STD; VRT; 718 BP. XX AC SCPV02062876; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22445.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-718 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-718 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 8d0e6b5e00ca7c48294547fe8b8ae9d5. DR ENA-CON; ML191262. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..718 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22445.1" XX SQ Sequence 718 BP; 235 A; 142 C; 129 G; 212 T; 0 other; tgggagtcca caattctgta tgcaagtaga cagcaagcaa cagtgtttgt aacctgaagg 60 actcagccta gtaacatatt agttactcta ctttgtctac atgcagaagc acacttcatc 120 aagagtgcac ataaactaga tttaagttca gtctctttga ttcaaattga tgtttctgtt 180 accatttgtg tcatgcacat aagatataaa agtattaaaa gtagaatgtt ttgagttaaa 240 gttgttaatt tttttaaaaa actcttttct gggactggag ggatggctca gtggctatgc 300 cattgcagag gaccagacac agttcaaacc atctacgccg aggctaacaa ccatctgtaa 360 ttcttgttct ataggactct gatgcattct gatctctgcc agcacacggt acacacatgg 420 atgcaggtac agcactcaga cacataaagt atcaaaaaga gaaaactctt ttctctccta 480 cttgtttcgt gccctagtct ttgagctcag tgattatgca gttcagaatc ggcatgactc 540 agaattggtt tatcagacac cgttctagaa aacagtagtt tcataacgat ttgtcaatta 600 gtgcaatgca ccaaaattac atacctgaca acttcagaat tcaaaaaatt aaaaaaaaaa 660 aagtaaaacg gcttttaagg tattttcccc ataaattaca tccaagtgag ccacagtg 718 // ID SCPV02062877; SV 1; linear; genomic DNA; STD; VRT; 718 BP. XX AC SCPV02062877; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22445.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-718 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-718 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 8d0e6b5e00ca7c48294547fe8b8ae9d5. DR ENA-CON; ML191262. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..718 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22445.2" XX SQ Sequence 718 BP; 235 A; 142 C; 129 G; 212 T; 0 other; tgggagtcca caattctgta tgcaagtaga cagcaagcaa cagtgtttgt aacctgaagg 60 actcagccta gtaacatatt agttactcta ctttgtctac atgcagaagc acacttcatc 120 aagagtgcac ataaactaga tttaagttca gtctctttga ttcaaattga tgtttctgtt 180 accatttgtg tcatgcacat aagatataaa agtattaaaa gtagaatgtt ttgagttaaa 240 gttgttaatt tttttaaaaa actcttttct gggactggag ggatggctca gtggctatgc 300 cattgcagag gaccagacac agttcaaacc atctacgccg aggctaacaa ccatctgtaa 360 ttcttgttct ataggactct gatgcattct gatctctgcc agcacacggt acacacatgg 420 atgcaggtac agcactcaga cacataaagt atcaaaaaga gaaaactctt ttctctccta 480 cttgtttcgt gccctagtct ttgagctcag tgattatgca gttcagaatc ggcatgactc 540 agaattggtt tatcagacac cgttctagaa aacagtagtt tcataacgat ttgtcaatta 600 gtgcaatgca ccaaaattac atacctgaca acttcagaat tcaaaaaatt aaaaaaaaaa 660 aagtaaaacg gcttttaagg tattttcccc ataaattaca tccaagtgag ccacagtg 718 // ID SCPV02062878; SV 1; linear; genomic DNA; STD; VRT; 758 BP. XX AC SCPV02062878; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22446.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-758 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-758 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2a453f516463fa18a88590cbd476a8bd. DR ENA-CON; ML191263. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..758 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22446.1" XX SQ Sequence 758 BP; 226 A; 152 C; 155 G; 225 T; 0 other; ggacaaaaag tagtacagga agagattatc agcataaaca ggcattaaat aggaaaccgt 60 cactctctga ctttttatgg acacaccctt cttgtttagg aaacagaaga ggatgagagt 120 acatctgtga tcagttgcta cgtgtggtgt caagtgtttt ctggaatgag atggtttgac 180 tattaccata ggtatagttc aaaatacaaa acaggacttg gaacctttat tgtaacaagc 240 ttgtacgtgt gtggtcatct cccccccgcc ccctcagtcc cccgaccccc aatgtaagga 300 acacctttga aggagaaaga tgaagtcttt gaggaataca ttcagaaata caggactata 360 ataaatacat gcctcttagt gcgcaactgt tgctaatacg gcttccttcc tttgaggcag 420 agcacataag tacagttagc atggctctga gacacagcac aggcttcctg accacataga 480 atactgcctt ccatcagggt gggctaagtt gccaaaaacg acaatcaggg atccaataag 540 caatcttggt cacttaagta aattttatat tgcctttttt atctttgaga agtatttgtc 600 ataaaggtgt agaaacaggc tgtcattcct tcctattttt cttgctttgg gtttaatagt 660 tgttcataat tagtgctgaa cacttgatta cagataaact tatggaactt ccagctgtaa 720 atctagaatt cttgagcttc atagctcttc agttgtgc 758 // ID SCPV02062879; SV 1; linear; genomic DNA; STD; VRT; 758 BP. XX AC SCPV02062879; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22446.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-758 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-758 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 2a453f516463fa18a88590cbd476a8bd. DR ENA-CON; ML191263. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..758 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22446.2" XX SQ Sequence 758 BP; 226 A; 152 C; 155 G; 225 T; 0 other; ggacaaaaag tagtacagga agagattatc agcataaaca ggcattaaat aggaaaccgt 60 cactctctga ctttttatgg acacaccctt cttgtttagg aaacagaaga ggatgagagt 120 acatctgtga tcagttgcta cgtgtggtgt caagtgtttt ctggaatgag atggtttgac 180 tattaccata ggtatagttc aaaatacaaa acaggacttg gaacctttat tgtaacaagc 240 ttgtacgtgt gtggtcatct cccccccgcc ccctcagtcc cccgaccccc aatgtaagga 300 acacctttga aggagaaaga tgaagtcttt gaggaataca ttcagaaata caggactata 360 ataaatacat gcctcttagt gcgcaactgt tgctaatacg gcttccttcc tttgaggcag 420 agcacataag tacagttagc atggctctga gacacagcac aggcttcctg accacataga 480 atactgcctt ccatcagggt gggctaagtt gccaaaaacg acaatcaggg atccaataag 540 caatcttggt cacttaagta aattttatat tgcctttttt atctttgaga agtatttgtc 600 ataaaggtgt agaaacaggc tgtcattcct tcctattttt cttgctttgg gtttaatagt 660 tgttcataat tagtgctgaa cacttgatta cagataaact tatggaactt ccagctgtaa 720 atctagaatt cttgagcttc atagctcttc agttgtgc 758 // ID SCPV02062880; SV 1; linear; genomic DNA; STD; VRT; 860 BP. XX AC SCPV02062880; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22447.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-860 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-860 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; b3bbf40dfb1230a123239dad5ffeff3a. DR ENA-CON; ML191264. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..860 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22447.1" XX SQ Sequence 860 BP; 237 A; 214 C; 193 G; 216 T; 0 other; ctttcttata tgggggtcat aagagctagc tctctgtttt gtgaagctta aagctgtgtt 60 tccctctgca caggagtcaa ctgagagctc aaacaccacc attgaggatg aagacgtgaa 120 agcacgaaaa caggagatca tcaaagtcac tgagcaactg attgaagcta tcaacaatgg 180 ggactttgag gcttacacaa aaatctgtga tccaggcctc actgcctttg aacctgaagc 240 attgggcaac ttagtggaag ggatggactt tcacagattc tactttgaaa atgctttgtc 300 caaaagcaat aaaccaatcc acacgatcat cctcaaccca cacgttcacc tggtagggga 360 tgacgcagcc tgcatcgcat acattcggct cacacagtac atggacggaa gcgggatgcc 420 aaagaccatg cagtcagaag agacgcgcgt gtggcaccgc cgtgatggga agtggcagaa 480 tgttcacttt caccgttcgg ggtcccccac agtacccatc aagccactct ctataccgaa 540 acatatacat tctgtcttca aggcaccagg cgggtgatcc tgggacgtcc tctcctcttc 600 atgcatgttt ctgagtgcat gaagttgtga aggttctacg tgtaatgcat atgtgacacg 660 tcatcttatc atgtgacacg tcatcttatc atgtattcct tcctgtacat tgtttacact 720 ccagctaccg gacgggatgt tccatgcaaa cttccgttac tgttggcaaa ctctagaggg 780 agctcagaca agaaaattcc acaataactc caagttcagc tgatccgtca ggtttctctg 840 tgtatgccaa gatccaaaag 860 // ID SCPV02062881; SV 1; linear; genomic DNA; STD; VRT; 860 BP. XX AC SCPV02062881; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22447.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-860 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-860 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; b3bbf40dfb1230a123239dad5ffeff3a. DR ENA-CON; ML191264. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..860 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22447.2" XX SQ Sequence 860 BP; 237 A; 214 C; 193 G; 216 T; 0 other; ctttcttata tgggggtcat aagagctagc tctctgtttt gtgaagctta aagctgtgtt 60 tccctctgca caggagtcaa ctgagagctc aaacaccacc attgaggatg aagacgtgaa 120 agcacgaaaa caggagatca tcaaagtcac tgagcaactg attgaagcta tcaacaatgg 180 ggactttgag gcttacacaa aaatctgtga tccaggcctc actgcctttg aacctgaagc 240 attgggcaac ttagtggaag ggatggactt tcacagattc tactttgaaa atgctttgtc 300 caaaagcaat aaaccaatcc acacgatcat cctcaaccca cacgttcacc tggtagggga 360 tgacgcagcc tgcatcgcat acattcggct cacacagtac atggacggaa gcgggatgcc 420 aaagaccatg cagtcagaag agacgcgcgt gtggcaccgc cgtgatggga agtggcagaa 480 tgttcacttt caccgttcgg ggtcccccac agtacccatc aagccactct ctataccgaa 540 acatatacat tctgtcttca aggcaccagg cgggtgatcc tgggacgtcc tctcctcttc 600 atgcatgttt ctgagtgcat gaagttgtga aggttctacg tgtaatgcat atgtgacacg 660 tcatcttatc atgtgacacg tcatcttatc atgtattcct tcctgtacat tgtttacact 720 ccagctaccg gacgggatgt tccatgcaaa cttccgttac tgttggcaaa ctctagaggg 780 agctcagaca agaaaattcc acaataactc caagttcagc tgatccgtca ggtttctctg 840 tgtatgccaa gatccaaaag 860 // ID SCPV02062882; SV 1; linear; genomic DNA; STD; VRT; 871 BP. XX AC SCPV02062882; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22448.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-871 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-871 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f3f3ea1323d14a9ab9864b91ffee71f4. DR ENA-CON; ML191265. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..871 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22448.1" XX SQ Sequence 871 BP; 296 A; 132 C; 223 G; 220 T; 0 other; gatgaagatt atgaaatggc tgtcaagaag cttaatggaa aactctatct ggatgactca 60 gaaaaaccat tggaagaaaa tctagcagat aatgacaagg aatgtgttat tgtttgggaa 120 aagaagccaa cagttgaaga aagagcaaaa gcagatactt taaagcttcc acctacattt 180 ttctgtggag tctgcagtga tactgatgag gacaatggaa atggggagga ttttcagtca 240 gagcttcgaa aagtctgtga agctcagaaa tcccatataa gaaaagtgac tgatagagtt 300 ggtattgagc atataggtga gactgaagta acaaaccctg ttggctgtaa gtctgaagaa 360 cctgattctg ataccaaaca cagtagctca tcccctgttt ctgggactat ggacaaacct 420 gtcgatttgt ccactagaaa agaaactgac atggaattcc caagcaaagg ggaaaacaag 480 cctgttttgt ttggatttgg aagtggtaca ggcctgtcat ttgcagactt ggcttcaagt 540 aattctggag attttgcttt tggctctaaa gataaaaatt tccagtgggc aaatactgga 600 gcagctgtgt ttggaacaca gacaacaagt aaaggtggcg aggatgaaga cggcagtgat 660 gaagatgtag ttcataatga ggacatccac tttgaaccta tagtctcctt accagaggta 720 gaagtgaaat ctggagagga agatgaagaa gttttgttca aagagagagc caaactttat 780 aggtgggaca gagatgtcag tcagtggaag gagagaggta ttggagatat aaagattctc 840 tggcatacaa tgaagaagta ttatcggatc c 871 // ID SCPV02062883; SV 1; linear; genomic DNA; STD; VRT; 871 BP. XX AC SCPV02062883; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22448.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-871 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-871 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f3f3ea1323d14a9ab9864b91ffee71f4. DR ENA-CON; ML191265. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..871 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22448.2" XX SQ Sequence 871 BP; 296 A; 132 C; 223 G; 220 T; 0 other; gatgaagatt atgaaatggc tgtcaagaag cttaatggaa aactctatct ggatgactca 60 gaaaaaccat tggaagaaaa tctagcagat aatgacaagg aatgtgttat tgtttgggaa 120 aagaagccaa cagttgaaga aagagcaaaa gcagatactt taaagcttcc acctacattt 180 ttctgtggag tctgcagtga tactgatgag gacaatggaa atggggagga ttttcagtca 240 gagcttcgaa aagtctgtga agctcagaaa tcccatataa gaaaagtgac tgatagagtt 300 ggtattgagc atataggtga gactgaagta acaaaccctg ttggctgtaa gtctgaagaa 360 cctgattctg ataccaaaca cagtagctca tcccctgttt ctgggactat ggacaaacct 420 gtcgatttgt ccactagaaa agaaactgac atggaattcc caagcaaagg ggaaaacaag 480 cctgttttgt ttggatttgg aagtggtaca ggcctgtcat ttgcagactt ggcttcaagt 540 aattctggag attttgcttt tggctctaaa gataaaaatt tccagtgggc aaatactgga 600 gcagctgtgt ttggaacaca gacaacaagt aaaggtggcg aggatgaaga cggcagtgat 660 gaagatgtag ttcataatga ggacatccac tttgaaccta tagtctcctt accagaggta 720 gaagtgaaat ctggagagga agatgaagaa gttttgttca aagagagagc caaactttat 780 aggtgggaca gagatgtcag tcagtggaag gagagaggta ttggagatat aaagattctc 840 tggcatacaa tgaagaagta ttatcggatc c 871 // ID SCPV02062884; SV 1; linear; genomic DNA; STD; VRT; 798 BP. XX AC SCPV02062884; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22449.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-798 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-798 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 7bb960c35a7203e51e388482eeba10cd. DR ENA-CON; ML191266. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..798 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22449.1" XX SQ Sequence 798 BP; 257 A; 172 C; 165 G; 204 T; 0 other; tgggataaga gtggttggtt tgtgtttttt tgttttttaa atcttgtttc tcatggttta 60 tttattgatg gcatattggt gggggggggg tgcattaaac agaaatggga gtttaaggaa 120 gagtatatga ttcacatctt actttataac gtaagcagaa agagcccttc tgtctctgca 180 gagactcaaa ggaagagcca gagcctggtc tgtcacggca gctgaacaca aagacgacaa 240 caggaaggac ctagtcacac agtcacagga gcagcccagg ctgctttacc attctcagtc 300 tcctccccca gcctcagtgc tgacattaga gatacagagc accaacccag gaatctagta 360 ttaatgacgg tcaacagcat ctcaggatac accacccaac aggaccaaga tatccaaact 420 caacaaagtc tttgtatttg ccagcaaaga gttaccactg ccctggctaa gaggaggaca 480 gagcagcaaa tactgagaca aggctggctt ctggtagtag taatgaatcc aaagtttgca 540 taagcaaggg aaggcttatc tcaggttgta cccctaaata acaaatggaa tgtggagctg 600 aaatctacac ttttattcac tcaactatac caagataaac atctttaaaa attaaaactt 660 acatgcaata agctttacat taaatgctga aaacagtctc ttggcccaga atctaaagat 720 cctccctttc ttccttacct cgaatttcaa agaaaagaaa actgaaaaac tgcctctgtg 780 aacacgacac ggatctct 798 // ID SCPV02062885; SV 1; linear; genomic DNA; STD; VRT; 798 BP. XX AC SCPV02062885; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22449.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-798 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-798 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 7bb960c35a7203e51e388482eeba10cd. DR ENA-CON; ML191266. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..798 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22449.2" XX SQ Sequence 798 BP; 257 A; 172 C; 165 G; 204 T; 0 other; tgggataaga gtggttggtt tgtgtttttt tgttttttaa atcttgtttc tcatggttta 60 tttattgatg gcatattggt gggggggggg tgcattaaac agaaatggga gtttaaggaa 120 gagtatatga ttcacatctt actttataac gtaagcagaa agagcccttc tgtctctgca 180 gagactcaaa ggaagagcca gagcctggtc tgtcacggca gctgaacaca aagacgacaa 240 caggaaggac ctagtcacac agtcacagga gcagcccagg ctgctttacc attctcagtc 300 tcctccccca gcctcagtgc tgacattaga gatacagagc accaacccag gaatctagta 360 ttaatgacgg tcaacagcat ctcaggatac accacccaac aggaccaaga tatccaaact 420 caacaaagtc tttgtatttg ccagcaaaga gttaccactg ccctggctaa gaggaggaca 480 gagcagcaaa tactgagaca aggctggctt ctggtagtag taatgaatcc aaagtttgca 540 taagcaaggg aaggcttatc tcaggttgta cccctaaata acaaatggaa tgtggagctg 600 aaatctacac ttttattcac tcaactatac caagataaac atctttaaaa attaaaactt 660 acatgcaata agctttacat taaatgctga aaacagtctc ttggcccaga atctaaagat 720 cctccctttc ttccttacct cgaatttcaa agaaaagaaa actgaaaaac tgcctctgtg 780 aacacgacac ggatctct 798 // ID SCPV02062886; SV 1; linear; genomic DNA; STD; VRT; 512 BP. XX AC SCPV02062886; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22450.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-512 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-512 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 00d15f4d6507f8651ad28cc4c81d2308. DR ENA-CON; ML191267. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..512 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22450.1" XX SQ Sequence 512 BP; 81 A; 149 C; 139 G; 143 T; 0 other; atggggcact gcttcagggg caaggcccgt tcagggcccc gggccacaag ctggacgcct 60 ctgtgtgcct ggtcagctgc cgtggcagag gtatgtttgg atggactggg agcagcccct 120 gggaccggct gcagctgaag ggcatcagcg ctttctgggg accgcccact ctcatgtggc 180 tgcaagtcct cctcatcgcc tacgcttttc atcttcctcc tttttcttat atggggtgtc 240 tgctcccaca cagctgtggg agcagcaact ctgtagtgga gaacctgggg ttgtgcccca 300 ccgggtgccg ggccgtgctc tgcctctctc acagctgcct ttgttttggg cccatgtaac 360 tctgaacagt aaaatttctt atgattctca aaattctcca gcttccgata cctatttcga 420 caggtttcac actcaaacat ggttcctcgt ccttgatgtg acttcttctt ctctaggtga 480 ggggtctgcg ctgctttgct ctgtactggt cc 512 // ID SCPV02062887; SV 1; linear; genomic DNA; STD; VRT; 512 BP. XX AC SCPV02062887; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22450.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-512 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-512 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 00d15f4d6507f8651ad28cc4c81d2308. DR ENA-CON; ML191267. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..512 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22450.2" XX SQ Sequence 512 BP; 81 A; 149 C; 139 G; 143 T; 0 other; atggggcact gcttcagggg caaggcccgt tcagggcccc gggccacaag ctggacgcct 60 ctgtgtgcct ggtcagctgc cgtggcagag gtatgtttgg atggactggg agcagcccct 120 gggaccggct gcagctgaag ggcatcagcg ctttctgggg accgcccact ctcatgtggc 180 tgcaagtcct cctcatcgcc tacgcttttc atcttcctcc tttttcttat atggggtgtc 240 tgctcccaca cagctgtggg agcagcaact ctgtagtgga gaacctgggg ttgtgcccca 300 ccgggtgccg ggccgtgctc tgcctctctc acagctgcct ttgttttggg cccatgtaac 360 tctgaacagt aaaatttctt atgattctca aaattctcca gcttccgata cctatttcga 420 caggtttcac actcaaacat ggttcctcgt ccttgatgtg acttcttctt ctctaggtga 480 ggggtctgcg ctgctttgct ctgtactggt cc 512 // ID SCPV02062888; SV 1; linear; genomic DNA; STD; VRT; 594 BP. XX AC SCPV02062888; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22451.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-594 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-594 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 44f9806bb8866214c4d074a26b84eee4. DR ENA-CON; ML191268. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..594 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22451.1" XX SQ Sequence 594 BP; 185 A; 114 C; 159 G; 136 T; 0 other; gtgagaggct gactgtatcc gaggatggat cccaagtaaa ccaccaggag gacccaaagc 60 acaatgacga ctatgtatat gacatttact atatggagat ggcccctcca gggtggattg 120 agaacatcat gtccgtgcag ccctacagcc aggaatggga gctggtaaat gatgatgagc 180 agtcagaaga tatttatgaa gatgaagatg atgaaaacag tgaaaataac tggcggaatg 240 agtacccgga tgaagaaagc agtgatagag atgaagattc cagaggctct gatgaataca 300 acagcctcag cgaggaggaa agaagctgcg agcgactgat gtggagcaaa taccctttgg 360 atgtacaaaa ggagtttgac tatgacagcc cccatggtct ggattcagat tgatggtttg 420 ctggtatttg tgagattctt ccatgggcct ttaaggctct gactacagag tcatcatctt 480 aggattccta actgaacatg tggaaagaaa agcctttacc ccagcacagt gaggacttcc 540 ttgtaagtga taagtgacgc caaacacgct gggtcatgtc tgtaattcca gcat 594 // ID SCPV02062889; SV 1; linear; genomic DNA; STD; VRT; 594 BP. XX AC SCPV02062889; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22451.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-594 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-594 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 44f9806bb8866214c4d074a26b84eee4. DR ENA-CON; ML191268. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..594 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22451.2" XX SQ Sequence 594 BP; 185 A; 114 C; 159 G; 136 T; 0 other; gtgagaggct gactgtatcc gaggatggat cccaagtaaa ccaccaggag gacccaaagc 60 acaatgacga ctatgtatat gacatttact atatggagat ggcccctcca gggtggattg 120 agaacatcat gtccgtgcag ccctacagcc aggaatggga gctggtaaat gatgatgagc 180 agtcagaaga tatttatgaa gatgaagatg atgaaaacag tgaaaataac tggcggaatg 240 agtacccgga tgaagaaagc agtgatagag atgaagattc cagaggctct gatgaataca 300 acagcctcag cgaggaggaa agaagctgcg agcgactgat gtggagcaaa taccctttgg 360 atgtacaaaa ggagtttgac tatgacagcc cccatggtct ggattcagat tgatggtttg 420 ctggtatttg tgagattctt ccatgggcct ttaaggctct gactacagag tcatcatctt 480 aggattccta actgaacatg tggaaagaaa agcctttacc ccagcacagt gaggacttcc 540 ttgtaagtga taagtgacgc caaacacgct gggtcatgtc tgtaattcca gcat 594 // ID SCPV02062890; SV 1; linear; genomic DNA; STD; VRT; 724 BP. XX AC SCPV02062890; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22452.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-724 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-724 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; b442acd67d5c7982799e26c595c18b6f. DR ENA-CON; ML191269. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..724 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22452.1" XX SQ Sequence 724 BP; 235 A; 176 C; 124 G; 189 T; 0 other; aacatggatg ccactaaagg aatatacagg ccaccaatga aaacacttcc tcttcacatt 60 gttatggcac caggtacaat cttttcagtc aggcttgctg tcaagtgcta gatttttaca 120 gcaggtgatg agacacagac aaacagcaag tgttttgtca caagcaatct cacaatgtaa 180 gcagtgcttg aatttccccc aagcaccggt gacagaaccg gcagatagcc tcctacagat 240 tcatcatcag ggtcaccaaa cagtcccaca gtccacagtc ctcagatgtc atggtgatgt 300 cgctgtgcac acaccaacac accctaacgt atttaaaaat taatccagtg agtaccaact 360 tcagaatact tcccttactg aaggaattca ttctaacaac catttctttc aacttcctag 420 aacacactgt actactgtgt gtcctaagct tggaacaaat catagttaag aaaaattcag 480 caagtggaaa tgaagatttt tcccccctta agcaaatagt ttaaataata gcatctaact 540 gcagtggtga taagtgacat ttccaaacaa tctccacatt taaaataaaa atagacaaag 600 tgcaaaattt ccacctttcc ttacattcta aatgggcact acagaacccg ctggtgtaca 660 aggcaaggtc actggcattc acgaggttgg ctttttatct tagagtggcc cgttcccatc 720 acat 724 // ID SCPV02062891; SV 1; linear; genomic DNA; STD; VRT; 724 BP. XX AC SCPV02062891; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22452.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-724 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-724 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; b442acd67d5c7982799e26c595c18b6f. DR ENA-CON; ML191269. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..724 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22452.2" XX SQ Sequence 724 BP; 235 A; 176 C; 124 G; 189 T; 0 other; aacatggatg ccactaaagg aatatacagg ccaccaatga aaacacttcc tcttcacatt 60 gttatggcac caggtacaat cttttcagtc aggcttgctg tcaagtgcta gatttttaca 120 gcaggtgatg agacacagac aaacagcaag tgttttgtca caagcaatct cacaatgtaa 180 gcagtgcttg aatttccccc aagcaccggt gacagaaccg gcagatagcc tcctacagat 240 tcatcatcag ggtcaccaaa cagtcccaca gtccacagtc ctcagatgtc atggtgatgt 300 cgctgtgcac acaccaacac accctaacgt atttaaaaat taatccagtg agtaccaact 360 tcagaatact tcccttactg aaggaattca ttctaacaac catttctttc aacttcctag 420 aacacactgt actactgtgt gtcctaagct tggaacaaat catagttaag aaaaattcag 480 caagtggaaa tgaagatttt tcccccctta agcaaatagt ttaaataata gcatctaact 540 gcagtggtga taagtgacat ttccaaacaa tctccacatt taaaataaaa atagacaaag 600 tgcaaaattt ccacctttcc ttacattcta aatgggcact acagaacccg ctggtgtaca 660 aggcaaggtc actggcattc acgaggttgg ctttttatct tagagtggcc cgttcccatc 720 acat 724 // ID SCPV02062892; SV 1; linear; genomic DNA; STD; VRT; 595 BP. XX AC SCPV02062892; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22453.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-595 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-595 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 9f2336afe7539e3413d14fb356dfe19b. DR ENA-CON; ML191270. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..595 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22453.1" XX SQ Sequence 595 BP; 131 A; 200 C; 125 G; 139 T; 0 other; gcctgagcgg ctcttcagcg tttgcgccgg cggctgccgc gtctctctcg gctcccgctt 60 cctttgaccg cctccccccc cggcccggcg gcgcccgcct cctccacggc cactccgcct 120 cttccctccc ttcgtccctt cttcctctcc cttttttcct tcttccttcc cctcctcgcc 180 gccaccgccc aggaccgccg gccgggggac gagctcggag cagcagccag agtttattaa 240 ccacttaacc tctcagaact gaacaaagac aacattgttc ctggaacgcc ctctttttaa 300 aaaagaaagc ataaccccta ctgtagaact aaatgcactg tgcatgaaac ttggaaaaaa 360 accaatgtat aagcctgttg acccttactc tcggatgcag tccacctata actacaacat 420 gagaggaggt gcttatcccc cgaggtactt ttacccattt ccagttccac ctttacttta 480 tcaagtggaa ctttctgtgg gaggacagca atttaatggc aaaggaaaga caagacaggc 540 tgcgaaacac gatgctgctg ccaaagcgtt gaggatcctg cagaatgagc ccctg 595 // ID SCPV02062893; SV 1; linear; genomic DNA; STD; VRT; 595 BP. XX AC SCPV02062893; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22453.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-595 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-595 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 9f2336afe7539e3413d14fb356dfe19b. DR ENA-CON; ML191270. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..595 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22453.2" XX SQ Sequence 595 BP; 131 A; 200 C; 125 G; 139 T; 0 other; gcctgagcgg ctcttcagcg tttgcgccgg cggctgccgc gtctctctcg gctcccgctt 60 cctttgaccg cctccccccc cggcccggcg gcgcccgcct cctccacggc cactccgcct 120 cttccctccc ttcgtccctt cttcctctcc cttttttcct tcttccttcc cctcctcgcc 180 gccaccgccc aggaccgccg gccgggggac gagctcggag cagcagccag agtttattaa 240 ccacttaacc tctcagaact gaacaaagac aacattgttc ctggaacgcc ctctttttaa 300 aaaagaaagc ataaccccta ctgtagaact aaatgcactg tgcatgaaac ttggaaaaaa 360 accaatgtat aagcctgttg acccttactc tcggatgcag tccacctata actacaacat 420 gagaggaggt gcttatcccc cgaggtactt ttacccattt ccagttccac ctttacttta 480 tcaagtggaa ctttctgtgg gaggacagca atttaatggc aaaggaaaga caagacaggc 540 tgcgaaacac gatgctgctg ccaaagcgtt gaggatcctg cagaatgagc ccctg 595 // ID SCPV02062894; SV 1; linear; genomic DNA; STD; VRT; 588 BP. XX AC SCPV02062894; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22454.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-588 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-588 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 6bed7538b6a41dd73ba9c73e954c6303. DR ENA-CON; ML191271. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..588 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22454.1" XX SQ Sequence 588 BP; 168 A; 130 C; 150 G; 140 T; 0 other; attggagaca accatgttta gtatttgagc attggttaaa tgctaaagaa aaatcgccgt 60 taaagcagtt ttctttttca ctgtcttttt cttttcgcgg ggaacccagc tgttcctgcg 120 agggccacct cctcaggaag accccgcagc tctcccgcgg cgcttctgca ggaggcagcg 180 acagtttcga gaacccgggc cttcccctcc cagtgcctcc gggggttccg gcgtttcagg 240 cgctgctgtt ttccgggaag ggcaggcgcg ctgggccttg gggagctgcg ctcggcgggc 300 ggacgcgggg gatcatggaa gctgataaag atgacacaca acaaattctt aaggagcatt 360 tgccagatga atttataaaa gatgaacaaa ataagggact aattgatgaa attacaaaga 420 aaaatattca gctaaagaag gagatccaaa agcttgaaac ggagttacaa gaggctacca 480 aagaattcca gattaaagag gatattcctg aaacaaagat gaaattctta tcagttgaaa 540 ctcctgagaa tgacagccag ttgtcaaata tctcctgttc gtttcaag 588 // ID SCPV02062895; SV 1; linear; genomic DNA; STD; VRT; 588 BP. XX AC SCPV02062895; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22454.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-588 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-588 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 6bed7538b6a41dd73ba9c73e954c6303. DR ENA-CON; ML191271. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..588 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22454.2" XX SQ Sequence 588 BP; 168 A; 130 C; 150 G; 140 T; 0 other; attggagaca accatgttta gtatttgagc attggttaaa tgctaaagaa aaatcgccgt 60 taaagcagtt ttctttttca ctgtcttttt cttttcgcgg ggaacccagc tgttcctgcg 120 agggccacct cctcaggaag accccgcagc tctcccgcgg cgcttctgca ggaggcagcg 180 acagtttcga gaacccgggc cttcccctcc cagtgcctcc gggggttccg gcgtttcagg 240 cgctgctgtt ttccgggaag ggcaggcgcg ctgggccttg gggagctgcg ctcggcgggc 300 ggacgcgggg gatcatggaa gctgataaag atgacacaca acaaattctt aaggagcatt 360 tgccagatga atttataaaa gatgaacaaa ataagggact aattgatgaa attacaaaga 420 aaaatattca gctaaagaag gagatccaaa agcttgaaac ggagttacaa gaggctacca 480 aagaattcca gattaaagag gatattcctg aaacaaagat gaaattctta tcagttgaaa 540 ctcctgagaa tgacagccag ttgtcaaata tctcctgttc gtttcaag 588 // ID SCPV02062896; SV 1; linear; genomic DNA; STD; VRT; 596 BP. XX AC SCPV02062896; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22455.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-596 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-596 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 22f536b8ebad0b72b3dd0c1111f3d20a. DR ENA-CON; ML191272. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..596 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22455.1" XX SQ Sequence 596 BP; 153 A; 145 C; 143 G; 155 T; 0 other; ggctcatgtg tggtcttagc acttggaaga cagagacaag aggattgctg aagacttacg 60 ccagccagat gtgaggctgg aggagggttg caaacggaag gaagaaaggc agtacactca 120 ggttccagac ctattccctg tgagcttgag aaaaggttgg gcaatggggc ccgaagggca 180 tgcacactca agtagcattg ctccttccaa attaaaagag cccagcatga tacttttaat 240 atatctgatg gtttaaattg tcgcctgccc cataataaat gaactatgct tgtagtcagg 300 gcccagcacg actgcttgga tacatattct ttctctcctg agctggccct ggtttgaaac 360 tgctctctta ggctgacctc cttccagttt ccctgaggcc atgtgtggcc tatgacagat 420 ggtcgctctg tttccctttg tccagctagg cctccctcct attcctcctc ctcgtcctcc 480 tcaggaaaaa gaactggaga gattaatatg agccaagtag gaaggaactt gaagattaaa 540 agtgttatgt gccctttctg ctctctgaac acccctgagc tccccatata agaaaa 596 // ID SCPV02062897; SV 1; linear; genomic DNA; STD; VRT; 596 BP. XX AC SCPV02062897; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22455.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-596 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-596 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 22f536b8ebad0b72b3dd0c1111f3d20a. DR ENA-CON; ML191272. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..596 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22455.2" XX SQ Sequence 596 BP; 153 A; 145 C; 143 G; 155 T; 0 other; ggctcatgtg tggtcttagc acttggaaga cagagacaag aggattgctg aagacttacg 60 ccagccagat gtgaggctgg aggagggttg caaacggaag gaagaaaggc agtacactca 120 ggttccagac ctattccctg tgagcttgag aaaaggttgg gcaatggggc ccgaagggca 180 tgcacactca agtagcattg ctccttccaa attaaaagag cccagcatga tacttttaat 240 atatctgatg gtttaaattg tcgcctgccc cataataaat gaactatgct tgtagtcagg 300 gcccagcacg actgcttgga tacatattct ttctctcctg agctggccct ggtttgaaac 360 tgctctctta ggctgacctc cttccagttt ccctgaggcc atgtgtggcc tatgacagat 420 ggtcgctctg tttccctttg tccagctagg cctccctcct attcctcctc ctcgtcctcc 480 tcaggaaaaa gaactggaga gattaatatg agccaagtag gaaggaactt gaagattaaa 540 agtgttatgt gccctttctg ctctctgaac acccctgagc tccccatata agaaaa 596 // ID SCPV02062898; SV 1; linear; genomic DNA; STD; VRT; 530 BP. XX AC SCPV02062898; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22456.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-530 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-530 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 842052e85b89cfd4ff82def7ad4df792. DR ENA-CON; ML191273. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..530 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22456.1" XX SQ Sequence 530 BP; 170 A; 114 C; 93 G; 153 T; 0 other; cttggaatcg ccacaaataa acattataat gttttgacag attccccaaa gaaacaacat 60 tttaagcccc atggataatg atccctgaga cttctcagtg cagaagaaac cctcccagga 120 tttcctgtct ctcatttact ttccttggtt ctttttatgt ctggtaaact ttgactgtac 180 acaggatcaa gtactcagac aaaatgacta cagagctaat atacagagta gcaattctat 240 agaaatgttg agtggaactt caaaaaaaaa taagacccat ataaaaaaga aatctatatg 300 cccctggaat tttgttataa atttacatta gcaataatga ccttggttca gtaacaatta 360 gcagccagca gcagccctat ggtgctttgg ccaacaacct cttccagtgg acacgaactg 420 gccatttatc ccaaggaagt gccccaaggt gggatcctcg gcttttgcca tctgattgtg 480 atttagcctc ggattttaaa agggacagac aatagatttt tctttccata 530 // ID SCPV02062899; SV 1; linear; genomic DNA; STD; VRT; 530 BP. XX AC SCPV02062899; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22456.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-530 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-530 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 842052e85b89cfd4ff82def7ad4df792. DR ENA-CON; ML191273. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..530 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22456.2" XX SQ Sequence 530 BP; 170 A; 114 C; 93 G; 153 T; 0 other; cttggaatcg ccacaaataa acattataat gttttgacag attccccaaa gaaacaacat 60 tttaagcccc atggataatg atccctgaga cttctcagtg cagaagaaac cctcccagga 120 tttcctgtct ctcatttact ttccttggtt ctttttatgt ctggtaaact ttgactgtac 180 acaggatcaa gtactcagac aaaatgacta cagagctaat atacagagta gcaattctat 240 agaaatgttg agtggaactt caaaaaaaaa taagacccat ataaaaaaga aatctatatg 300 cccctggaat tttgttataa atttacatta gcaataatga ccttggttca gtaacaatta 360 gcagccagca gcagccctat ggtgctttgg ccaacaacct cttccagtgg acacgaactg 420 gccatttatc ccaaggaagt gccccaaggt gggatcctcg gcttttgcca tctgattgtg 480 atttagcctc ggattttaaa agggacagac aatagatttt tctttccata 530 // ID SCPV02062900; SV 1; linear; genomic DNA; STD; VRT; 470 BP. XX AC SCPV02062900; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22457.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-470 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-470 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 51bb18c3ccafe22bc3c34774279e67e7. DR ENA-CON; ML191274. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..470 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22457.1" XX SQ Sequence 470 BP; 102 A; 134 C; 132 G; 102 T; 0 other; agctccgtcc cgcccgcggg ccggctccgc cctcacctcc cggccgcggc tgccctctgc 60 ccgggttgtc caagatggag ggcgctccac cggggtcgct cgccctccgg ctcctgctgt 120 tcgtggcgct acccgcctcc ggctggctga cgacgggcgc ccccgagccg ccgccgctgt 180 ccggagcccc acaggacggc atcagaatta atgtaactac actgaaagat gatggggaca 240 tatctaaaca gcaggttgtt cttaacataa cctatgagag tggacaggtg tatgtaaatg 300 acttacctgt aaatagtggt gtaacccgaa taagctgtca gactttgata gtgaagagtg 360 aaaatctgga aaaattggag gagaaacact attttggaat tgtcactgtg aggatcttag 420 ttctcgagag gcctgtgacg tacagtgcca gctcccagct gattgtcatc 470 // ID SCPV02062901; SV 1; linear; genomic DNA; STD; VRT; 470 BP. XX AC SCPV02062901; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22457.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-470 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-470 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 51bb18c3ccafe22bc3c34774279e67e7. DR ENA-CON; ML191274. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..470 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22457.2" XX SQ Sequence 470 BP; 102 A; 134 C; 132 G; 102 T; 0 other; agctccgtcc cgcccgcggg ccggctccgc cctcacctcc cggccgcggc tgccctctgc 60 ccgggttgtc caagatggag ggcgctccac cggggtcgct cgccctccgg ctcctgctgt 120 tcgtggcgct acccgcctcc ggctggctga cgacgggcgc ccccgagccg ccgccgctgt 180 ccggagcccc acaggacggc atcagaatta atgtaactac actgaaagat gatggggaca 240 tatctaaaca gcaggttgtt cttaacataa cctatgagag tggacaggtg tatgtaaatg 300 acttacctgt aaatagtggt gtaacccgaa taagctgtca gactttgata gtgaagagtg 360 aaaatctgga aaaattggag gagaaacact attttggaat tgtcactgtg aggatcttag 420 ttctcgagag gcctgtgacg tacagtgcca gctcccagct gattgtcatc 470 // ID SCPV02062902; SV 1; linear; genomic DNA; STD; VRT; 694 BP. XX AC SCPV02062902; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22458.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-694 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-694 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d9c0d3f282cffe7c5655102a08ede96d. DR ENA-CON; ML191275. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..694 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22458.1" XX SQ Sequence 694 BP; 99 A; 202 C; 258 G; 135 T; 0 other; tatatgggag aacccggaag ctggggaccc acgtgggagg ctgggaggtg actgtcttgg 60 ctcgagggtc aggttgtggg cagtccttgt gaggggacga agtgtgggag gtcaacgggt 120 cctccccagc tgtctgtcag tggagtctcg ggcgctgctg gagcaatgcg gcgactctgg 180 acggtcgggt gcttcagtcg cctgattgcc cgccatttct cctcggtggc tcggcgcggg 240 gaacggcctg gcggggagga gctgagtcgc ttgcttctgg atgacctggc gccggcccag 300 cggctggaac gtctatttgg cttgtcccca tgcctcctgg ccttgcgggc tgcccgtcgg 360 cgcgtggccc ggctcctgct ccaggccggt aaggctgggc tgcaggggga gcgagccgag 420 ctgctccggg tggccgaggc tcggggcatc cctgtcttga ggcccaggcg tcagaaactg 480 gatgccttgt gcggctacca agtgcaccag ggcgtgtgca tggaggtgag cccgctgcga 540 ccccggcctt gcgatgaggc ggcagacacg agctcaggcg acgaccctca gcagctgtgg 600 ctcgtccttg aggggctcca ggatccccgc aatcttgggg ctgtgatgcg ctccgctcac 660 ttcctcggcg tagacagagt ccccatataa gaaa 694 // ID SCPV02062903; SV 1; linear; genomic DNA; STD; VRT; 694 BP. XX AC SCPV02062903; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22458.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-694 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-694 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; d9c0d3f282cffe7c5655102a08ede96d. DR ENA-CON; ML191275. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..694 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22458.2" XX SQ Sequence 694 BP; 99 A; 202 C; 258 G; 135 T; 0 other; tatatgggag aacccggaag ctggggaccc acgtgggagg ctgggaggtg actgtcttgg 60 ctcgagggtc aggttgtggg cagtccttgt gaggggacga agtgtgggag gtcaacgggt 120 cctccccagc tgtctgtcag tggagtctcg ggcgctgctg gagcaatgcg gcgactctgg 180 acggtcgggt gcttcagtcg cctgattgcc cgccatttct cctcggtggc tcggcgcggg 240 gaacggcctg gcggggagga gctgagtcgc ttgcttctgg atgacctggc gccggcccag 300 cggctggaac gtctatttgg cttgtcccca tgcctcctgg ccttgcgggc tgcccgtcgg 360 cgcgtggccc ggctcctgct ccaggccggt aaggctgggc tgcaggggga gcgagccgag 420 ctgctccggg tggccgaggc tcggggcatc cctgtcttga ggcccaggcg tcagaaactg 480 gatgccttgt gcggctacca agtgcaccag ggcgtgtgca tggaggtgag cccgctgcga 540 ccccggcctt gcgatgaggc ggcagacacg agctcaggcg acgaccctca gcagctgtgg 600 ctcgtccttg aggggctcca ggatccccgc aatcttgggg ctgtgatgcg ctccgctcac 660 ttcctcggcg tagacagagt ccccatataa gaaa 694 // ID SCPV02062904; SV 1; linear; genomic DNA; STD; VRT; 710 BP. XX AC SCPV02062904; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22459.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-710 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-710 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4149865e94c742ff9cc6b82df209d615. DR ENA-CON; ML191276. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..710 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22459.1" XX SQ Sequence 710 BP; 188 A; 149 C; 138 G; 235 T; 0 other; gtggagtgtc tcatgttggg catttttagt agcccagact ctgttcttca tttgaatgtt 60 tcacacattt ttgttcacag ttaatcttcc aagtttacta ttcaagtcag aaattcagat 120 gacatttcta gtggtttgct gttttggttt tttatgtttt ttggtttttt ttgaggtttc 180 atttcttaca caggtgtctt catcaccatc acttctacac tgggggaaaa acaatctcct 240 ttgtgagaat cactgcatgt atttatggcg aaaatatttc tgaaagtcta gagtgataca 300 agtgagcaca agaagttggt cagcttgcct atggagtgct ggcaataaac tctgaacatt 360 ccacaagcct gagctgaacc taggctccct tggaagctga acagacatag gaacatggga 420 ttgccagctg aatcctgtga ccatctagtt atggacctca gagatagatc agcgtggcct 480 aaagccattt caagtacaaa aatgaattgg actatagcaa cataaatttg aacacagtaa 540 cttttcctct ttgcctgagg gcatttgtaa actcggcaga agtaacttga cctcatgtct 600 actgcagagc tcactgcatt cacccctcct catcctttgc ttccttcccc ttgcctagtc 660 agtagttcat aacttagtgt tccttttgct tcagaaatct gaaagaaaat 710 // ID SCPV02062905; SV 1; linear; genomic DNA; STD; VRT; 710 BP. XX AC SCPV02062905; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22459.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-710 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-710 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 4149865e94c742ff9cc6b82df209d615. DR ENA-CON; ML191276. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..710 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22459.2" XX SQ Sequence 710 BP; 188 A; 149 C; 138 G; 235 T; 0 other; gtggagtgtc tcatgttggg catttttagt agcccagact ctgttcttca tttgaatgtt 60 tcacacattt ttgttcacag ttaatcttcc aagtttacta ttcaagtcag aaattcagat 120 gacatttcta gtggtttgct gttttggttt tttatgtttt ttggtttttt ttgaggtttc 180 atttcttaca caggtgtctt catcaccatc acttctacac tgggggaaaa acaatctcct 240 ttgtgagaat cactgcatgt atttatggcg aaaatatttc tgaaagtcta gagtgataca 300 agtgagcaca agaagttggt cagcttgcct atggagtgct ggcaataaac tctgaacatt 360 ccacaagcct gagctgaacc taggctccct tggaagctga acagacatag gaacatggga 420 ttgccagctg aatcctgtga ccatctagtt atggacctca gagatagatc agcgtggcct 480 aaagccattt caagtacaaa aatgaattgg actatagcaa cataaatttg aacacagtaa 540 cttttcctct ttgcctgagg gcatttgtaa actcggcaga agtaacttga cctcatgtct 600 actgcagagc tcactgcatt cacccctcct catcctttgc ttccttcccc ttgcctagtc 660 agtagttcat aacttagtgt tccttttgct tcagaaatct gaaagaaaat 710 // ID SCPV02062906; SV 1; linear; genomic DNA; STD; VRT; 848 BP. XX AC SCPV02062906; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22460.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-848 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-848 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; af2e7baad1eaf7ccc349d276b3b38a6d. DR ENA-CON; ML191277. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..848 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22460.1" XX SQ Sequence 848 BP; 159 A; 280 C; 262 G; 147 T; 0 other; cccaggggac agcgactcag ccaatgggaa gtcgggggtg tgaggaggag gcgggcgccg 60 ctggaagagg cccgggccgg ggcgatggtg gggctcgggg tcccgccggt gactccccgc 120 cccgccccgg cgcgctgcgt cccgggcccc cggccgctca cctccccttc accgtcacac 180 cgcgcttccg gatgagctcg tccagggaca ggtccgccat cttgtcgcgg ggcccagaga 240 caagacgcag cgagcgaagc ccggacatga gattaaacta cttccgcttc ccctcccagc 300 tccgcgcggc aacggcgctg cgtcgcgcga cccttcccgg gcgcgcgccg caaagagggc 360 gagaaggaaa ggcgcgcggt tcccacaatc ctttgcgtcc gaagactcta tatttgcata 420 cgcgacggcg attaccctgg acgatttcta ccccgaaaac aaagctcacg ctgaaaagag 480 acacgggtgg aagcgtcagg agggtctgga accgcggtgt ggcttgacct gaatccgggg 540 tggaactgca gccttgctca ccccgaccta aatagcatcc gtctcggctc ccccctctag 600 ctgctccact ttctcgtcat gccttaaact tatgagtaag gaaaataacg atccggggtg 660 acgcccgggt cctcactgct aatgtgagaa gaatttttgc gcgggcacag gtcgccccta 720 gggtgactcg cctacttagc gagatgcccg ggtgctgtga tctgcccgat cccacccacc 780 tgctgatgtg gcaaagacaa agagattgtg cttgctccct gcccgagctg ctccgtgcac 840 gggccttg 848 // ID SCPV02062907; SV 1; linear; genomic DNA; STD; VRT; 848 BP. XX AC SCPV02062907; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22460.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-848 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-848 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; af2e7baad1eaf7ccc349d276b3b38a6d. DR ENA-CON; ML191277. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..848 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22460.2" XX SQ Sequence 848 BP; 159 A; 280 C; 262 G; 147 T; 0 other; cccaggggac agcgactcag ccaatgggaa gtcgggggtg tgaggaggag gcgggcgccg 60 ctggaagagg cccgggccgg ggcgatggtg gggctcgggg tcccgccggt gactccccgc 120 cccgccccgg cgcgctgcgt cccgggcccc cggccgctca cctccccttc accgtcacac 180 cgcgcttccg gatgagctcg tccagggaca ggtccgccat cttgtcgcgg ggcccagaga 240 caagacgcag cgagcgaagc ccggacatga gattaaacta cttccgcttc ccctcccagc 300 tccgcgcggc aacggcgctg cgtcgcgcga cccttcccgg gcgcgcgccg caaagagggc 360 gagaaggaaa ggcgcgcggt tcccacaatc ctttgcgtcc gaagactcta tatttgcata 420 cgcgacggcg attaccctgg acgatttcta ccccgaaaac aaagctcacg ctgaaaagag 480 acacgggtgg aagcgtcagg agggtctgga accgcggtgt ggcttgacct gaatccgggg 540 tggaactgca gccttgctca ccccgaccta aatagcatcc gtctcggctc ccccctctag 600 ctgctccact ttctcgtcat gccttaaact tatgagtaag gaaaataacg atccggggtg 660 acgcccgggt cctcactgct aatgtgagaa gaatttttgc gcgggcacag gtcgccccta 720 gggtgactcg cctacttagc gagatgcccg ggtgctgtga tctgcccgat cccacccacc 780 tgctgatgtg gcaaagacaa agagattgtg cttgctccct gcccgagctg ctccgtgcac 840 gggccttg 848 // ID SCPV02062908; SV 1; linear; genomic DNA; STD; VRT; 587 BP. XX AC SCPV02062908; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22461.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-587 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-587 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 3d6be3ab1e8bfa78f77d0d05fa4de06a. DR ENA-CON; ML191278. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..587 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22461.1" XX SQ Sequence 587 BP; 141 A; 171 C; 151 G; 124 T; 0 other; gcgaggagcg tcgccgagag cgcagaattg agatggagga gaacaagaga cagggcaggt 60 atcccgaggt ccacattccc ctcgagaacc tacagcagtt tccggccttc aattcctata 120 cctgtccctc tgattctgct ttgggtccca ccagcactga gggccatggg gccctatccc 180 tgtctcctct gagcagaagt ccaggctccc atgcagactt tctgctgacc cctctgtcac 240 ccactgccag tcagggcagt ccttccttct gcgttgggag tctggaagac gactctccct 300 tcctttcctt tgcccagatg ctgagggttg gcaaagcaaa agcagacggg tggcccaaaa 360 ctgctccaaa gaaagatgac aatagcttgg ttcctcccgc ccctgtggac agtgatgggg 420 agagtgacaa ttctgaccgt gttcctgtgc ccagtttcca aaattccttc agccaagcta 480 tcgaagcagc cttcatgaaa ttggacacac cagctacttc agaccccctc tctgaagaca 540 gaggagggaa gaaaaggaag agacagaagc agaagctgct gttcagc 587 // ID SCPV02062909; SV 1; linear; genomic DNA; STD; VRT; 587 BP. XX AC SCPV02062909; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22461.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-587 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-587 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 3d6be3ab1e8bfa78f77d0d05fa4de06a. DR ENA-CON; ML191278. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..587 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22461.2" XX SQ Sequence 587 BP; 141 A; 171 C; 151 G; 124 T; 0 other; gcgaggagcg tcgccgagag cgcagaattg agatggagga gaacaagaga cagggcaggt 60 atcccgaggt ccacattccc ctcgagaacc tacagcagtt tccggccttc aattcctata 120 cctgtccctc tgattctgct ttgggtccca ccagcactga gggccatggg gccctatccc 180 tgtctcctct gagcagaagt ccaggctccc atgcagactt tctgctgacc cctctgtcac 240 ccactgccag tcagggcagt ccttccttct gcgttgggag tctggaagac gactctccct 300 tcctttcctt tgcccagatg ctgagggttg gcaaagcaaa agcagacggg tggcccaaaa 360 ctgctccaaa gaaagatgac aatagcttgg ttcctcccgc ccctgtggac agtgatgggg 420 agagtgacaa ttctgaccgt gttcctgtgc ccagtttcca aaattccttc agccaagcta 480 tcgaagcagc cttcatgaaa ttggacacac cagctacttc agaccccctc tctgaagaca 540 gaggagggaa gaaaaggaag agacagaagc agaagctgct gttcagc 587 // ID SCPV02062910; SV 1; linear; genomic DNA; STD; VRT; 483 BP. XX AC SCPV02062910; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22462.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-483 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-483 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 8801651975c6016650c008e7b334a489. DR ENA-CON; ML191279. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..483 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22462.1" XX SQ Sequence 483 BP; 97 A; 170 C; 127 G; 89 T; 0 other; ccaaggtccc caaggccggt gttgagggtt gtgggggagg ggtgagaaac agaaccggag 60 acccgggtga ggacccagcc cagagccatc taagactatc aactctggag gttcctcctc 120 cagctcccgc tagcctggga cttatatgaa tccgaggaag agagagagaa agagaggaga 180 ggcccttagc ctcgcactgc accccaagct gttgtggcga cgggtgtgcg gcactctgcc 240 cgctaccatt cgcgtccttc gccctcgatt ggctacgtgg ggaccgcagc accccttacc 300 tgtgactccg cggactgcct cagcctccat gctccggtac ggagtttgcc acctagtaag 360 cctctacctc cccaccccct ccccccacca gaaccaccaa cctttctacc aagagttccc 420 caggggctgc cccgccctgc cacctccccg gaggctttct ctttctcccc catataagaa 480 agg 483 // ID SCPV02062911; SV 1; linear; genomic DNA; STD; VRT; 483 BP. XX AC SCPV02062911; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22462.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-483 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-483 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; 8801651975c6016650c008e7b334a489. DR ENA-CON; ML191279. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..483 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22462.2" XX SQ Sequence 483 BP; 97 A; 170 C; 127 G; 89 T; 0 other; ccaaggtccc caaggccggt gttgagggtt gtgggggagg ggtgagaaac agaaccggag 60 acccgggtga ggacccagcc cagagccatc taagactatc aactctggag gttcctcctc 120 cagctcccgc tagcctggga cttatatgaa tccgaggaag agagagagaa agagaggaga 180 ggcccttagc ctcgcactgc accccaagct gttgtggcga cgggtgtgcg gcactctgcc 240 cgctaccatt cgcgtccttc gccctcgatt ggctacgtgg ggaccgcagc accccttacc 300 tgtgactccg cggactgcct cagcctccat gctccggtac ggagtttgcc acctagtaag 360 cctctacctc cccaccccct ccccccacca gaaccaccaa cctttctacc aagagttccc 420 caggggctgc cccgccctgc cacctccccg gaggctttct ctttctcccc catataagaa 480 agg 483 // ID SCPV02062912; SV 1; linear; genomic DNA; STD; VRT; 858 BP. XX AC SCPV02062912; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22463.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-858 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-858 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f57ef2745aa5bc44d387541ce11d5ed4. DR ENA-CON; ML191280. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..858 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22463.1" XX SQ Sequence 858 BP; 173 A; 239 C; 273 G; 173 T; 0 other; tagccgtgtt atattacaga ttggggttct attctcagtt ctgcatgaag tgaccttgtg 60 ctttctctca gttactttat gtataaagtg agtgcaaaat attacttcct gtaaggtggg 120 gagtaggagg tatatgaata gtagttagga aggacacaga aatattaaca atactcaggg 180 aagagctggg actagaattc acactctcct gaggtgccgt tcagtgcaat gtgccccaag 240 ctacaggatg ttatatggca gaatctcttt ctctccctgc acttaccccc accccctcag 300 gtgtgcattt aagtatttta taactaagag ctagaaagat accgtactta gcaccagggg 360 cggctctagg caccagcaaa acaagctggt gcctacggcg gcaaaatata gggggcggca 420 aaaggctggg gtcccagctc atcctgccgc tgcgagccga ggagcagccc gtcccctgca 480 gccggggcag ggctgtgcta ccggctccgc ttgggggaga gggatggccc cttaccggta 540 gcgcggcccc gcctggctgc agaagacggg ccgctcctcc tccgcttgtt cgcgcccggg 600 tcctaacagc ccagcagcag cttggctggg gggggcgggg ctgagttccg ccccgctcag 660 agccgcgtgg tgagggggcg gggcggcgag ctctgggcca agcggaggca gctgagctca 720 gcgcggagct cgcagcccca cccctcccca cgcgactctg agcggggaag agctcagtcc 780 cttccggagc catgcggctg cctcgctggg cagggccgct tctcgaggtg cgcggtgagc 840 cgggggtaag cagccggg 858 // ID SCPV02062913; SV 1; linear; genomic DNA; STD; VRT; 858 BP. XX AC SCPV02062913; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22463.2, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-858 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-858 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f57ef2745aa5bc44d387541ce11d5ed4. DR ENA-CON; ML191280. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled genome CC (Cuora_amboinensis-2.0), the total assembly N50 contig and scaffold CC lengths are 47.1 Kb and 345.1 Kb, respectively. This genome appears CC to have higher repeat content than most other turtle genomes CC sequenced to date (approx. 25%), and have a larger estimated genome CC size (approx. 3.1Gb). For questions regarding this CC Cuora_amboinensis-2.0 assembly please contact Robert Fulton, CC McDonnell Genome Institute at Washington University School of CC Medicine, St. Louis. CC Data use: The Cuora_amboinensis-2.0 assembly sequence is made CC freely available to the community by the McDonnell Genome Institute CC at Washington University School of Medicine, with the following CC understanding: 1. The data may be freely downloaded, used in CC analyses, and repackaged in databases. 2. Users are free to use the CC data in scientific papers analyzing these data if the providers of CC these data are properly cited. 3. Any redistribution of the data CC should carry this notice. CC Cuora amboinensis Sequence and Assembly Credits: Sequencing Funds CC - Costs of sequencing the genome was graciously donated by John CC Bermingham. DNA source - Robert Fulton, McDonnell Genome Institute CC at Washington University School of Medicine, St. Louis Blood CC extraction - Lauren Augustine, Curator of Herpetology, Saint Louis CC Zoo, Saint Louis, MO, and Mary Duncan, Staff Pathologist, Saint CC Louis Zoo, Saint Louis, MO Genome Sequence - The McDonnell Genome CC Institute, Washington University School of Medicine. Sequence CC Assembly - The McDonnell Genome Institute, Washington University CC School of Medicine. CC ##Genome-Assembly-Data-START## CC Assembly Method :: Supernova v. 2.1.1 CC Genome Coverage :: 58x CC Sequencing Technology :: Illumina CC ##Genome-Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..858 FT /organism="Cuora amboinensis" FT /mol_type="genomic DNA" FT /country="USA:St. Louis Zoo, St Louis, MO" FT /specimen_voucher="SLZ ID 105011" FT /collection_date="2018-09-21" FT /sex="male" FT /dev_stage="adult" FT /tissue_type="blood" FT /db_xref="taxon:74909" FT /submitter_seqid="Contig22463.2" XX SQ Sequence 858 BP; 173 A; 239 C; 273 G; 173 T; 0 other; tagccgtgtt atattacaga ttggggttct attctcagtt ctgcatgaag tgaccttgtg 60 ctttctctca gttactttat gtataaagtg agtgcaaaat attacttcct gtaaggtggg 120 gagtaggagg tatatgaata gtagttagga aggacacaga aatattaaca atactcaggg 180 aagagctggg actagaattc acactctcct gaggtgccgt tcagtgcaat gtgccccaag 240 ctacaggatg ttatatggca gaatctcttt ctctccctgc acttaccccc accccctcag 300 gtgtgcattt aagtatttta taactaagag ctagaaagat accgtactta gcaccagggg 360 cggctctagg caccagcaaa acaagctggt gcctacggcg gcaaaatata gggggcggca 420 aaaggctggg gtcccagctc atcctgccgc tgcgagccga ggagcagccc gtcccctgca 480 gccggggcag ggctgtgcta ccggctccgc ttgggggaga gggatggccc cttaccggta 540 gcgcggcccc gcctggctgc agaagacggg ccgctcctcc tccgcttgtt cgcgcccggg 600 tcctaacagc ccagcagcag cttggctggg gggggcgggg ctgagttccg ccccgctcag 660 agccgcgtgg tgagggggcg gggcggcgag ctctgggcca agcggaggca gctgagctca 720 gcgcggagct cgcagcccca cccctcccca cgcgactctg agcggggaag agctcagtcc 780 cttccggagc catgcggctg cctcgctggg cagggccgct tctcgaggtg cgcggtgagc 840 cgggggtaag cagccggg 858 // ID SCPV02062914; SV 1; linear; genomic DNA; STD; VRT; 666 BP. XX AC SCPV02062914; XX PR Project:PRJNA511034; XX DT 11-JUN-2019 (Rel. 141, Created) DT 11-JUN-2019 (Rel. 141, Last updated, Version 1) XX DE Cuora amboinensis Contig22464.1, whole genome shotgun sequence. XX KW WGS. XX OS Cuora amboinensis (Amboina box turtle) OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Archelosauria; Testudines; Cryptodira; Durocryptodira; Testudinoidea; OC Geoemydidae; Geoemydinae; Cuora. XX RN [1] RP 1-666 RA Minx P., Bermingham J.; RT ; RL Submitted (16-JAN-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX RN [2] RP 1-666 RA Minx P., Bermingham J.; RT ; RL Submitted (20-MAR-2019) to the INSDC. RL McDonnell Genome Institute, Washington University School of Medicine, 4444 RL Forest Park, St. Louis, MO 63108, USA XX DR MD5; f253868aa4ae93c56ac0aa15add3fced. DR ENA-CON; ML191281. DR BioSample; SAMN10722981. XX CC This Cuora_amboinensis-2.0 assembly replaces the CC Cuora_amboinensis-1.0 assembly, which was an assisted assembly CC (ref: Cuora mccordi), and prone to scaffolding errors. Use the 1.0 CC version at your own risk. The Asian box turtle DNA was derived from CC blood of a single male (SLZ ID 105011) at the Saint Louis Zoo, CC Saint Louis, MO. Sequences were generated on the 10X Genomics CC Chromium sequencing platform to an approx. 58X coverage based on a CC genome size of 2.6 Gb. The sequences were assembled by the de novo CC assembly program, Supernova v2.1.1, which estimated the genome size CC to be 3.1Mb. Of the 2.5 Gb assembled