ID LC457975; SV 1; linear; other DNA; STD; SYN; 720 BP. XX AC LC457975; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct Gamillus-S gene for green fluorescent protein, complete DE cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-720 RA Nagai T., Matsuda T., Shinoda H.; RT ; RL Submitted (17-JAN-2019) to the INSDC. RL Contact:Tomoki Matsuda Osaka University, The Institute of Scientific & RL Industrial Research, Department of Biomolecular Science and Engineering; RL F-301, The Institute of Scientific & Industrial Research, Osaka University, RL 8-1, Mihogaoka, Ibaraki, Osaka 567-0047, Japan URL RL :https://www.sanken.osaka-u.ac.jp/labs/bse/english/ index.html#topics XX RN [2] RA Shinoda H., Matsuda T., Nagai T.; RT "Acid-Tolerant Monomeric Photoswitchable GFP from Olindias formosa: RT rsGamillus-S"; RL Unpublished. XX DR MD5; f333b64ca13b145dfc30f8ae5a1d5c3e. XX FH Key Location/Qualifiers FH FT source 1..720 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..720 FT /codon_start=1 FT /transl_table=11 FT /gene="Gamillus-S" FT /product="green fluorescent protein" FT /protein_id="BBI28097.1" FT /translation="MVSKGEEASGRALFQYPMTSKIELNGEINGKKFKVAGEGFTPSSG FT RFNMHAYCTTGDLPMSWVVIASPLQYGFHMFAHYPEDITHFFQECFPGSYTLDRTLRME FT GDGTLTTHHEYSLEDGCVTSKTTLNASGFDPKGATMTKSFVKQLPCEVKITPHGPNGIR FT LTSTVLYLKEDGTIQIGTQDCIVTPVGGRKVTQPKAHFLHVQIIQKKDPNDTRDHIVQT FT ELAVAGNLWHGMDELYK" XX SQ Sequence 720 BP; 178 A; 243 C; 196 G; 103 T; 0 other; atggtgagca agggcgagga ggcatctggc agagccctgt tccagtaccc catgaccagc 60 aagatcgagc tgaacggcga gatcaacggc aagaaattca aggtggccgg cgagggcttc 120 acccccagca gcggcagatt caacatgcac gcctactgca ccaccggcga cctgcctatg 180 agctgggtcg tgattgccag ccccctccag tacggcttcc acatgttcgc ccactacccc 240 gaggacatca cacacttttt ccaggaatgc ttccccggca gctacaccct ggaccggacc 300 ctgagaatgg aaggcgacgg caccctgacc acccaccacg agtacagcct ggaggacggc 360 tgcgtgacct ccaagaccac cctgaatgcc agcggcttcg accctaaggg cgccaccatg 420 accaagagct tcgtgaaaca actgccttgc gaggtgaaga tcacccccca cggccccaac 480 ggcatcagac tgaccagcac cgtgctgtac ctgaaggagg atggcaccat ccagatcggc 540 acccaggact gcatcgtgac ccctgtgggc ggaaggaaag tgacccagcc caaggcccac 600 ttcctgcacg tccagatcat ccagaagaag gaccccaacg acacccggga ccacatcgtg 660 cagacagaac tggccgtggc cggcaatctg tggcacggca tggacgagct gtacaagtaa 720 // ID LC457976; SV 1; linear; other DNA; STD; SYN; 720 BP. XX AC LC457976; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct Gamillus-F gene for green fluorescent protein, complete DE cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-720 RA Shinoda H., Matsuda T., Nagai T.; RT ; RL Submitted (17-JAN-2019) to the INSDC. RL Contact:Tomoki Matsuda Osaka University, The Institute of Scientific & RL Industrial Research, Department of Biomolecular Science and Engineering; RL F-301, The Institute of Scientific & Industrial Research, Osaka University, RL 8-1, Mihogaoka, Ibaraki, Osaka 567-0047, Japan URL RL :https://www.sanken.osaka-u.ac.jp/labs/bse/english/ index.html XX RN [2] RA Shinoda H., Matsuda T., Nagai T.; RT "Acid-Tolerant Monomeric Photoswitchable GFP from Olindias formosa: RT rsGamillus-F"; RL Unpublished. XX DR MD5; b0614710a30710df3a63b8fcc890517f. XX FH Key Location/Qualifiers FH FT source 1..720 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..720 FT /codon_start=1 FT /transl_table=11 FT /gene="Gamillus-F" FT /product="green fluorescent protein" FT /protein_id="BBI28098.1" FT /translation="MVSKGEEASGRALFQYPMTSKIELNGEINGKKFKVAGEGFTPSSG FT RFHMHAYCTTGDLPMSWVVIASPLQYGFHMFAHYPEDITHFFQECFPGSYTLDRTLRME FT GDGTLTTHHEYSLEDGCVTSKTTLNASGFDPKGATMTKSFVKQLPCEVKITPHGPNGIR FT LTSTVLYLKEDGTIQIGTQDCIVTPVGGRKVTQPKAHFLHAQIIQKKDPNDTRDHIVQT FT ELAVAGNLWHGMDELYK" XX SQ Sequence 720 BP; 177 A; 245 C; 196 G; 102 T; 0 other; atggtgagca agggcgagga ggcatctggc agagccctgt tccagtaccc catgaccagc 60 aagatcgagc tgaacggcga gatcaacggc aagaaattca aggtggccgg cgagggcttc 120 acccccagca gcggcagatt ccacatgcac gcctactgca ccaccggcga cctgcctatg 180 agctgggtcg tgattgccag ccccctccag tacggcttcc acatgttcgc ccactacccc 240 gaggacatca cacacttttt ccaggaatgc ttccccggca gctacaccct ggaccggacc 300 ctgagaatgg aaggcgacgg caccctgacc acccaccacg agtacagcct ggaggacggc 360 tgcgtgacct ccaagaccac cctgaatgcc agcggcttcg accctaaggg cgccaccatg 420 accaagagct tcgtgaaaca actgccttgc gaggtgaaga tcacccccca cggccccaac 480 ggcatcagac tgaccagcac cgtgctgtac ctgaaggagg atggcaccat ccagatcggc 540 acccaggact gcatcgtgac ccctgtgggc ggaaggaaag tgacccagcc caaggcccac 600 ttcctgcacg cccagatcat ccagaagaag gaccccaacg acacccggga ccacatcgtg 660 cagacagaac tggccgtggc cggcaatctg tggcacggca tggacgagct gtacaagtaa 720 // ID LC566707; SV 1; linear; other DNA; STD; SYN; 888 BP. XX AC LC566707; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct gene for polytope fusion protein, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-888 RA Sadeghi S., Bandehpour M.; RT ; RL Submitted (04-JUL-2020) to the INSDC. RL Contact:Sahar Sadeghi School of Advanced Technologies in Medicine, RL Department of Medical Biotechnology; Velenjak, Shahid Beheshti University RL of Medical Sciences, Tehran, Iran, Tehran, Tehran 98, Iran URL RL :http://sbmu.ac.ir/ XX RN [2] RA Sadeghi S., Bandehpour M.; RT "Computational designed an inhaled polypeptide vaccine against Influenza RT virus type A and Streptococcus pneumonia with chitin and chitosan adjuvants RT and Evaluation of them in BALB/c mice"; RL Unpublished. XX DR MD5; a5cda1b5f9ad93773f75aad5a582fec7. XX FH Key Location/Qualifiers FH FT source 1..888 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..888 FT /codon_start=1 FT /transl_table=11 FT /product="polytope fusion protein" FT /note="epitopes from Influenza A virus HA, M2, amd NP FT genes" FT /protein_id="BCH36770.1" FT /translation="MEVLVLWGIHHPPNSKGGGSLYEKVKSQLGGGSIYSTVASSLGPG FT PGVRSAKLRMVTGLRNIHHALLESMGIYQILAIYSTGGGSISCFLLCVVLLGFIHHAAG FT YFKMRTGKHHALSRISIYWTIKFERQRGYFKMRTGKSSIMRGPGPGIIGILHLILWILD FT RLGGGSNIIGILHLIHHALDPLAIAANIGGGSYRKEQQSAVDADDGHKFERQHKSCLPA FT CVYGPAVAGGGSFYIQMCTELHHAAAYERMCNILGPGPGKFQTAAQKAMMDQVRGGGSK FT ETAAAKFERQHMDS" XX SQ Sequence 888 BP; 171 A; 238 C; 225 G; 254 T; 0 other; atggaagttc tggttctgtg gggtatccac cacccgccga actctaaagg tggtggttct 60 ctgtacgaaa aagttaaatc tcagctgggt ggtggttcta tctactctac cgttgcttct 120 tctctgggtc cgggtccggg tgttcgttct gctaaactgc gtatggttac cggtctgcgt 180 aacatccacc acgctctgct ggaatctatg ggtatctacc agatcctggc tatctactct 240 accggtggtg gttctatctc ttgcttcctg ctgtgcgttg ttctgctggg tttcatccac 300 cacgctgctg gttacttcaa aatgcgtacc ggtaaacacc acgctctgtc tcgtatctct 360 atctactgga ccatcaaatt cgaacgtcag cgtggttact tcaaaatgcg taccggtaaa 420 tcttctatca tgcgtggtcc gggtccgggt atcatcggta tcctgcacct gatcctgtgg 480 atcctggacc gtctgggtgg tggttctaac atcatcggta tcctgcacct gatccaccac 540 gctctggacc cgctggctat cgctgctaac atcggtggtg gttcttaccg taaagaacag 600 cagtctgctg ttgacgctga cgacggtcac aaattcgaac gtcagcacaa atcttgcctg 660 ccggcttgcg tttacggtcc ggctgttgct ggtggtggtt ctttctacat ccagatgtgc 720 accgaactgc accacgctgc tgcttacgaa cgtatgtgca acatcctggg tccgggtccg 780 ggtaaattcc agaccgctgc tcagaaagct atgatggacc aggttcgtgg tggtggttct 840 aaagaaaccg ctgctgctaa attcgaacgt cagcacatgg actcttaa 888 // ID LC566708; SV 1; linear; other DNA; STD; SYN; 984 BP. XX AC LC566708; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct gene for polytope fusion protein, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-984 RA Sadeghi S., Bandehpour M.; RT ; RL Submitted (04-JUL-2020) to the INSDC. RL Contact:Sahar Sadeghi School of Advanced Technologies in Medicine, RL Department of Medical Biotechnology; Velenjak, Shahid Beheshti University RL of Medical Sciences, Tehran, Iran, Tehran, Tehran 98, Iran URL RL :http://sbmu.ac.ir/ XX RN [2] RA Sadeghi S., Bandehpour M.; RT "Computational designed an inhaled polypeptide vaccine against Influenza RT virus type A and Streptococcus pneumonia with chitin and chitosan adjuvants RT and Evaluation of them in BALB/c mice"; RL Unpublished. XX DR MD5; 1afbf833816e76c8866b5e7f0ec69326. XX FH Key Location/Qualifiers FH FT source 1..984 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..984 FT /codon_start=1 FT /transl_table=11 FT /product="polytope fusion protein" FT /note="epitopes from Streptococcus pneumoniae LytA, Ply, FT ZmpB, and NanA genes" FT /protein_id="BCH36771.1" FT /translation="MLYIELLRNLAGGGSSDHVDPYPYLAKGGGSYYFDSSGYMHHAAY FT YLDAKEGAKFERQAETYAAVELIESHSTKGPGPGGWNAETYAAVELIESGGGSDYRVVV FT DPVKPAYSDGGGSYPQVKDVYVQHHALNGVLSRTSLHHAATSLRSGNFIGPGPGYTYKA FT PLAQQALPETGGGSVGAMGLVVLPSAGAVGGGSAFGLLTVGSLLLIGGGSTYHYVNKEI FT HHALYSTASYNALGPGPGNKLKTAIDALNVDKTKFERQGNKTRASLLVPKVDYGGGSPL FT VYISSVAYHHAAKQIYYTVSVDAVKNPGGGSKETAAAKFERQHMDS" XX SQ Sequence 984 BP; 192 A; 264 C; 249 G; 279 T; 0 other; atgctgtaca tcgaactgct gcgtaacctg gctggtggtg gttcttctga ccacgttgac 60 ccgtacccgt acctggctaa aggtggtggt tcttactact tcgactcttc tggttacatg 120 caccacgctg cttactacct ggacgctaaa gaaggtgcta aattcgaacg tcaggctgaa 180 acctacgctg ctgttgaact gatcgaatct cactctacca aaggtccggg tccgggtggt 240 tggaacgctg aaacctacgc tgctgttgaa ctgatcgaat ctggtggtgg ttctgactac 300 cgtgttgttg ttgacccggt taaaccggct tactctgacg gtggtggttc ttacccgcag 360 gttaaagacg tttacgttca gcaccacgct ctgaacggtg ttctgtctcg tacctctctg 420 caccacgctg ctacctctct gcgttctggt aacttcatcg gtccgggtcc gggttacacc 480 tacaaagctc cgctggctca gcaggctctg ccggaaaccg gtggtggttc tgttggtgct 540 atgggtctgg ttgttctgcc gtctgctggt gctgttggtg gtggttctgc tttcggtctg 600 ctgaccgttg gttctctgct gctgatcggt ggtggttcta cctaccacta cgttaacaaa 660 gaaatccacc acgctctgta ctctaccgct tcttacaacg ctctgggtcc gggtccgggt 720 aacaaactga aaaccgctat cgacgctctg aacgttgaca aaaccaaatt cgaacgtcag 780 ggtaacaaaa cccgtgcttc tctgctggtt ccgaaagttg actacggtgg tggttctccg 840 ctggtttaca tctcttctgt tgcttaccac cacgctgcta aacagatcta ctacaccgtt 900 tctgttgacg ctgttaaaaa cccgggtggt ggttctaaag aaaccgctgc tgctaaattc 960 gaacgtcagc acatggactc ttaa 984 // ID MN612110; SV 1; linear; other DNA; STD; SYN; 456 BP. XX AC MN612110; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct PVF4-dAb gene, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-456 RA Madrid R., Garcia-Garcia A., Martin R., Garcia T.; RT ; RL Submitted (25-OCT-2019) to the INSDC. RL Nutrition and Food Science, University Complutense of Madrid, Avda. Puerta RL de Hierro s/n, Madrid, Madrid 28040, Spain XX DR MD5; 0843e893d4144993f5e41b5e94110980. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..456 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /clone="PVF4" FT /db_xref="taxon:32630" FT CDS <1..456 FT /codon_start=1 FT /transl_table=11 FT /transl_except=(pos:94..96,aa:Gln) FT /product="PVF4-dAb" FT /note="anti-Pistachio vera PVF4-dAb antibody with c-Myc and FT VSV tags; expression in Escherichia coli TG1 amber FT suppressor strain allows read through of amber stop codon" FT /protein_id="QLI42924.1" FT /translation="QVQLLESGGGLVQPGGSLRLSCAASGVSLNNQVMSWVRQAPGKGL FT EWVSTIRTQNGSTYYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAANHSTNA FT YVGFLGQGTLVTVSSAAAEQKLISEEDLNSAAHYTDIEMNRLGKGAA" FT V_region 1..357 FT /note="immunoglobulin heavy chain variable region" FT misc_feature 76..99 FT /note="Region: H1 hypervariable region" FT misc_feature 151..174 FT /note="Region: H2 hypervariable region" FT misc_feature 289..325 FT /note="Region: H3 hypervariable region" FT misc_feature 367..396 FT /note="Region: Myc (human c-Myc proto-oncogene) epitope FT tag" FT misc_feature 412..444 FT /note="Region: epitope tag from vesicular stomatitis virus FT G protein" XX SQ Sequence 456 BP; 110 A; 117 C; 134 G; 95 T; 0 other; caggtgcagc tgttggagtc tgggggaggc ttggtacagc ctggggggtc cctgcgtctc 60 tcctgtgcag cctccggagt tagccttaac aattaggtta tgagctgggt ccgccaggct 120 ccagggaagg gtctagagtg ggtatcaacc attcgtacgc aaaacggtag cacatactac 180 gcagactccg tgaagggccg gttcaccatc tcccgtgaca attccaagaa cacgctgtat 240 ctgcaaatga acagcctgcg tgccgaggac accgcggtat attattgcgc ggctaatcat 300 agtactaacg cctacgtggg gtttttgggt cagggaaccc tggtcaccgt ctcgagcgcg 360 gccgcagaac aaaaactcat ctcagaagag gatctgaatt cggccgcaca ttatacagac 420 atagagatga accgacttgg aaagggggcc gcatag 456 // ID MN623414; SV 1; linear; other DNA; STD; SYN; 1770 BP. XX AC MN623414; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct glucose oxidase GoxAh gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1770 RA Mou H., Liu Z., Yuan M.; RT "A thermostable glucose oxidase from Aspergillus heteromophus CBS 117.55 RT with broad pH stability and digestive enzyme resistance"; RL Unpublished. XX RN [2] RP 1-1770 RA Mou H., Liu Z., Yuan M.; RT ; RL Submitted (28-OCT-2019) to the INSDC. RL Ocean University of China, College of Food Science and Engineering, 5 RL Yushan Road, Qingdao, Shandong 266003, China XX DR MD5; 274c74d39b5276ceb0af72bc0698f0f2. XX FH Key Location/Qualifiers FH FT source 1..1770 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Aspergillus heteromophus CBS 117.55" FT /db_xref="taxon:32630" FT CDS 1..1770 FT /codon_start=1 FT /transl_table=11 FT /product="glucose oxidase GoxAh" FT /EC_number="1.1.3.4" FT /note="codon-optimized for Pichia pastoris expression" FT /protein_id="QLI42895.1" FT /translation="MPHYARRSLLEANLLTDPSKVAEHTVDYIIAGGGLTGLTAAARLS FT ENPNITVLVIETGFYESERGPIIEDLNTYGDIFGTTVDHAYETVELATNNRTEVIRSGN FT GLGGSTLVNGGTWTRPHKAQIDSWESVFGNDGWNWDTLVAYSHQVETVRAPNEKQIAAG FT HYFDPSCHGQNGSVHAGPRDTGESYSPIMKALMSTVQDQGVPIQQDLSCGNPHGVSMFP FT NSLNEEQTRSDAAREWLLPNFQRPNLQVLTGQRVGKVLLDQTGETPQAVGVEFGTHNNT FT KYNVYANHEVLLAAGSTVSPLILEYSGIGMKSILDSVGIETIVDLPVGLNLQDQTTTPV FT TSRITSAGDGQGQAAYFATFNETFGDYAPQAHELLNTKLAQWASETVSRGGFHNVTALT FT IQYENYRDWIVTHDVAYSELFLDTAGSISFTVWDLLPFTRGYVHILDKDPYLHRYAYNP FT QYLLNELDLLGQAAACKLARELSNSGEMQTYFAGEDVPGDNLAYDAGLDDWAEYIRYNF FT RPNYHGVGTCSMMPKELGGVVDSTARVYGVQGLRVIDGSIPPTQVSAHVMTVFYGMALK FT VSEAVLEDYAASL" XX SQ Sequence 1770 BP; 409 A; 425 C; 430 G; 506 T; 0 other; ttgccacact acgctagaag atccttgttg gaggctaact tgttgactga cccatccaag 60 gttgctgagc acactgtgga ctacattatt gctggtggtg gtttgaccgg tttgactgct 120 gctgctagat tgtctgagaa cccaaacatc accgtcctgg ttatcgagac tggtttctac 180 gaatctgaga gaggtccaat catcgaggac ttgaacactt acggtgacat cttcggtact 240 actgttgacc acgcttacga gactgttgag ttggctacca acaacagaac cgaggttatc 300 agatccggta acggtcttgg tggttccact ttggttaacg gtggtacttg gactagacca 360 cacaaggctc aaattgactc ttgggagtcc gttttcggta acgatggttg gaactgggat 420 accttggttg cttactctca ccaggttgag actgttagag ccccaaacga gaagcaaatt 480 gctgctggtc actacttcga cccatcttgt catggtcaaa acggttccgt tcatgctggt 540 ccaagagaca ctggtgaatc ttactcccca atcatgaagg ccttgatgtc cactgttcaa 600 gaccagggtg ttccaattca acaggacttg tcttgtggta acccacacgg tgtttctatg 660 ttcccaaact ccttgaacga ggaacagact agatctgacg ctgctagaga atggttgctg 720 ccaaacttcc aaagaccaaa cttgcaggtc ctgactggtc agagagttgg taaggttttg 780 ttggaccaga ctggtgagac tccacaagct gttggtgttg agtttggtac tcacaacaac 840 accaagtaca acgtctacgc caaccacgaa gtcttgttgg ctgctggttc tactgtttcc 900 ccactgatct tggaatactc cggtatcggt atgaagtcca tcttggactc cgtcggtatt 960 gagactatcg ttgacttgcc agtcggtttg aacttgcaag accagactac tactccagtg 1020 acctccagaa ttacttctgc tggtgatggt caaggtcagg ctgcttactt tgctaccttc 1080 aacgaaacct tcggtgacta cgctcctcaa gctcatgagt tgttgaacac taagttggct 1140 caatgggcct ccgaaactgt ttctagaggt ggtttccaca acgttactgc tctgactatc 1200 cagtacgaga actacagaga ctggatcgtt actcacgacg ttgcctactc tgagttgttc 1260 ttggataccg ctggttccat ctccttcact gtttgggatt tgctgccatt caccagaggt 1320 tacgtccaca ttttggacaa ggacccatac ctgcacagat acgcttacaa cccacagtac 1380 ttgttgaacg agttggactt gttgggtcaa gctgctgctt gtaagttggc cagagaattg 1440 tctaactccg gtgagatgca gacttacttc gccggtgaag atgttccagg tgacaacttg 1500 gcttacgatg ctggtttgga tgattgggcc gagtacatca gatacaactt cagacctaac 1560 taccacggtg tcggtacttg ttccatgatg ccaaaagaac ttggtggtgt cgttgactcc 1620 actgccagag tttatggtgt tcagggtttg agagttatcg acggttccat tccaccaact 1680 caagtttccg ctcacgtcat gaccgttttc tacggtatgg ctttgaaggt ttccgaggct 1740 gttttggagg attacgctgc ttccttgtaa 1770 // ID MN658769; SV 1; linear; other DNA; STD; SYN; 6609 BP. XX AC MN658769; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct hTfR::Syb::GFP gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-6609 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 4eadc0d5b4552c42714ff5aa8879abd0. XX FH Key Location/Qualifiers FH FT source 1..6609 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..2674,3848..6609) FT /note="pAWG" FT regulatory 96..2649 FT /note="ActinC5" FT /regulatory_class="promoter" FT 5'UTR 2675..2695 FT /note="Syn21" FT CDS 2696..3847 FT /codon_start=1 FT /transl_table=11 FT /product="hTfR::Syb::GFP" FT /protein_id="QGY73534.1" FT /translation="MMDQARSAFSNLFGGEPLSYTRFSLARQVDGDNSHVEMKLAVDEE FT ENADNNTKANVTKPKRCSGSICYGTIAVIVFFLIGFMIGYLGGEQPPQYQYPPQYMQPP FT PPPPQQPAGGQSSLVDGAGAGDGAGGSAGAGDHGGVMVSKGEELFTGVVPILVELDGDV FT NGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQH FT DFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHK FT LEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH FT YLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYKHRST" FT misc_feature 2696..2953 FT /note="cytosolic domain of the human transferrin receptor" FT misc_feature 2885..2953 FT /note="trans-membrane segment" FT misc_feature 2954..3115 FT /note="lumenal domain of Drosophila synaptobrevin" FT misc_feature 3116..3847 FT /note="EGFP" FT 3'UTR 3848..4570 FT /note="SV40" XX SQ Sequence 6609 BP; 1864 A; 1509 C; 1548 G; 1688 T; 0 other; gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact 60 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat 120 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca 180 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc 240 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat 300 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag 360 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc 420 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc 480 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg 540 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt 600 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc 660 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt 720 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt 780 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat 840 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt 900 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa 960 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg 1020 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg 1080 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc 1140 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc 1200 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt 1260 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact 1320 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc 1380 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat 1440 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg 1500 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca 1560 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt 1620 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag 1680 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct 1740 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt 1800 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca 1860 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc 1920 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct 1980 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta 2040 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa 2100 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat 2160 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac 2220 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac 2280 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga 2340 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt 2400 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg 2460 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc 2520 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc 2580 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa 2640 tccagagacc ccggatccag atatcgacaa gtttaactta aaaaaaaaaa tcaaaatgat 2700 ggatcaagct agatcagcat tctctaactt gtttggtgga gaaccattgt catatacccg 2760 gttcagcctg gctcggcaag tagatggcga taacagtcat gtggagatga aacttgctgt 2820 agatgaagaa gaaaatgctg acaataacac aaaggccaat gtcacaaaac caaaaaggtg 2880 tagtggaagt atctgctatg ggactattgc tgtgatcgtc tttttcttga ttggatttat 2940 gattggctac ttgggcgggg agcagccgcc acagtaccag tatcccccac agtacatgca 3000 gccaccgccg ccgccaccgc agcagccagc cggaggacag tcatcgctgg tggatggggc 3060 aggagcagga gacggagcag gaggatcggc aggagctggc gatcacggcg gcgtgatggt 3120 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 3180 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 3240 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 3300 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 3360 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 3420 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 3480 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 3540 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 3600 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 3660 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 3720 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 3780 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agcaccggtc 3840 cacgtgacgt aagctagcag gatctttgtg aaggaacctt acttctgtgg tgtgacataa 3900 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta 3960 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact 4020 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa 4080 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag 4140 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat 4200 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct 4260 gcactgctat acaagaaaat tatggaaaaa tattctgtaa cctttataag taggcataac 4320 agttataatc ataacatact gttttttctt actccacaca ggcatagagt gtctgctatt 4380 aataactatg ctcaaaaatt gtgtaccttt agctttttaa tttgtaaagg ggttaataag 4440 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga 4500 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa 4560 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag 4620 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 4680 actcatcaat gtatcttatc atgtctggat cccgtttaaa ctacgcgtaa ttcaaacagg 4740 gttctggcgt cgttctcgta ctgttttccc caggccagtg ctttagcgtt attgaaaaag 4800 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 4860 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 4920 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 4980 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 5040 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 5100 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 5160 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 5220 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 5280 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 5340 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 5400 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 5460 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 5520 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 5580 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 5640 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 5700 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 5760 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 5820 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 5880 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 5940 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 6000 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 6060 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 6120 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 6180 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 6240 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 6300 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 6360 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 6420 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 6480 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 6540 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 6600 agcggaaga 6609 // ID MN658770; SV 1; linear; other DNA; STD; SYN; 6235 BP. XX AC MN658770; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct 3xFlag::SNAP25 gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-6235 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 15a2a4d841195182cdcb89c88328bff0. XX FH Key Location/Qualifiers FH FT source 1..6235 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..2835,3457..6235) FT /note="pAFW" FT regulatory 96..2649 FT /note="ActinC5" FT /regulatory_class="promoter" FT CDS 2764..3456 FT /codon_start=1 FT /transl_table=11 FT /product="3xFlag::SNAP25" FT /protein_id="QGY73535.1" FT /translation="MDYKDHDGDYKDHDIDYKDDDDKHMAEDADMRNELEEMQRRADQL FT ADESLESTRRMLQLVEESKDAGIRTLVMLDEQGEQLDRVEEGMNHINQDMKEAEKNLKD FT LGKCCGLFICPCNKLKSSDAYKKAWGNNQDGVVASQPARVVDEREQMAISGGFIRRVTN FT DARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQR FT ATKMLGSG" FT misc_feature 2764..2835 FT /note="3xFLAG tag" FT misc_feature 2836..3456 FT /note="SNAP25 from human" FT 3'UTR 3457..4196 FT /note="SV40" XX SQ Sequence 6235 BP; 1809 A; 1330 C; 1447 G; 1649 T; 0 other; gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact 60 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat 120 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca 180 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc 240 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat 300 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag 360 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc 420 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc 480 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg 540 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt 600 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc 660 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt 720 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt 780 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat 840 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt 900 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa 960 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg 1020 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg 1080 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc 1140 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc 1200 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt 1260 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact 1320 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc 1380 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat 1440 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg 1500 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca 1560 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt 1620 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag 1680 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct 1740 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt 1800 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca 1860 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc 1920 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct 1980 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta 2040 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa 2100 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat 2160 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac 2220 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac 2280 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga 2340 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt 2400 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg 2460 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc 2520 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc 2580 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa 2640 tccagagacc ccggatccag atatcgaggc ctgtctagag aagcttgttc gaatctcgag 2700 tgcgcgcttc cggaggtata cacctaggcg gtaccactgc agtgaattcg gagctccgcc 2760 accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat 2820 gacgatgaca agcacatggc cgaggacgca gacatgcgca atgagctgga ggagatgcag 2880 cgaagggctg accagttggc tgatgagtcg ctggaaagca cccgtcgtat gctgcaactg 2940 gttgaagaga gtaaagatgc tggtatcagg actttggtta tgttggatga acaaggagaa 3000 caactcgatc gtgtcgaaga aggcatgaac catatcaacc aagacatgaa ggaggctgag 3060 aaaaatttaa aagatttagg gaaatgctgt ggccttttca tatgtccttg taacaagctt 3120 aaatcaagtg atgcttacaa aaaagcctgg ggcaataatc aggacggagt ggtggccagc 3180 cagcctgctc gtgtagtgga cgaacgggag cagatggcca tcagtggcgg cttcatccgc 3240 agggtaacaa atgatgcccg agaaaatgaa atggatgaaa acctagagca ggtgagcggc 3300 atcatcggga acctccgtca catggccctg gatatgggca atgagatcga tacacagaat 3360 cgccagatcg acaggatcat ggagaaggct gattccaaca aaaccagaat tgatgaggcc 3420 aaccaacgtg caacaaagat gctgggaagt ggttaacttg tacaaagtgg tgacgtaagc 3480 tagcaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg acaaactacc 3540 tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat gtgttaaact 3600 actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg aatgggagca 3660 gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc catctagtga 3720 tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga gaaaggtaga 3780 agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg tgtttagtaa 3840 tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac tgctatacaa 3900 gaaaattatg gaaaaatatt ctgtaacctt tataagtagg cataacagtt ataatcataa 3960 catactgttt tttcttactc cacacaggca tagagtgtct gctattaata actatgctca 4020 aaaattgtgt acctttagct ttttaatttg taaaggggtt aataaggaat atttgatgta 4080 tagtgccttg actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt 4140 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg 4200 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 4260 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 4320 cttatcatgt ctggatcccg tttaaactac gcgtaattca aacagggttc tggcgtcgtt 4380 ctcgtactgt tttccccagg ccagtgcttt agcgttattg aaaaaggaag agtatgagta 4440 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 4500 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 4560 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 4620 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 4680 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 4740 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 4800 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 4860 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 4920 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 4980 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 5040 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 5100 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 5160 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 5220 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 5280 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 5340 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 5400 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 5460 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 5520 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 5580 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 5640 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 5700 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 5760 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 5820 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 5880 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 5940 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 6000 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 6060 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 6120 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 6180 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga 6235 // ID MN658771; SV 1; linear; other DNA; STD; SYN; 7741 BP. XX AC MN658771; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct QF2::V5::SNAP25::syntaxin gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-7741 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; ead4a0aa4210e01ba4740010e38c540c. XX FH Key Location/Qualifiers FH FT source 1..7741 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..2834,4990..7741) FT /note="pAFW" FT regulatory 96..2649 FT /note="ActinC5" FT /regulatory_class="promoter" FT CDS 2764..4989 FT /codon_start=1 FT /transl_table=11 FT /product="QF2::V5::SNAP25::syntaxin" FT /protein_id="QGY73536.1" FT /translation="MDYKDHDGDYKDHDIDYKDDDDKQMPPKRKTLNAAAEANAHADGH FT ADGNADGHVANTAASSNNARFADLTNIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCR FT AAREKCDGIQPACFPCVSQGRSCTYQASPKKRGVQTGYIRTLELALAWMFENVARSEDA FT LHNLLVRDAGQGSALLVGKDSPAAERLHARWATSRVNKSITRLLATASIMPHVMEQPLS FT TSINPVNDRFNGIPNPTPYNSDAALDAITQTNDYGSVNTHGILSTYPPPATHLNEASVA FT LAPGGAPPRPPPPYVDSTTNHPPYHSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDV FT DPQFMTNLGFVPGCNFSDINTYEQGKPIPNPLLGLDSTARENEMDENLEQVSGIIGNLR FT HMALDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSGDDDDKQMTKDRLAA FT LHAAQSDDEEETEVAVNVDGHDSYMDDFFAQVEEIRGMIDKVQDNVEEVKKKHSAILSA FT PQTDEKTKQELEDLMADIKKNANRVRGKLKGIEQNIEQEEQQNKSSADLRIRKTQHSTL FT SRKFVEVMTEYNRTQTDYRERCKGRIQRQLEITGRPTNDDELEKMLEEGNSSVFTQGII FT METQQAKQTLADIEARHQDIMKLETSIKELHDMFMDMAMLVESQGEMIDRIEYHVEHAM FT DYVQTATQDTKKALKYQSKARRKKIMILICLTVLGILAASYVSSYFM" FT misc_feature 2764..2834 FT /note="3xFLAG tag" FT misc_feature 2836..3855 FT /note="QF2" FT misc_feature 3856..3897 FT /note="1xV5 tag" FT misc_feature 3898..4095 FT /note="encodes amino acids 141-206 of SNAP25 from human" FT misc_feature 4096..4110 FT /note="1xFLAG tag" FT misc_feature 4114..4989 FT /note="Drosophila syntaxin" FT 3'UTR 4990..5702 FT /note="SV40" XX SQ Sequence 7741 BP; 2179 A; 1792 C; 1842 G; 1928 T; 0 other; gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact 60 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat 120 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca 180 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc 240 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat 300 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag 360 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc 420 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc 480 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg 540 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt 600 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc 660 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt 720 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt 780 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat 840 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt 900 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa 960 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg 1020 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg 1080 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc 1140 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc 1200 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt 1260 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact 1320 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc 1380 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat 1440 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg 1500 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca 1560 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt 1620 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag 1680 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct 1740 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt 1800 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca 1860 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc 1920 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct 1980 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta 2040 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa 2100 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat 2160 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac 2220 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac 2280 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga 2340 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt 2400 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg 2460 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc 2520 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc 2580 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa 2640 tccagagacc ccggatccag atatcgaggc ctgtctagag aagcttgttc gaatctcgag 2700 tgcgcgcttc cggaggtata cacctaggcg gtaccactgc agtgaattcg gagctccgcc 2760 accatggact acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat 2820 gacgatgaca agcaaatgcc gcctaaacgc aagacactca atgccgctgc cgaagccaat 2880 gcccacgctg atggccatgc tgatggcaat gctgatggtc atgtcgctaa cactgcagca 2940 agcagcaaca acgcccgttt tgcggacttg accaacattg acacacccgg cctcggccct 3000 accacgacga cgttacttgt cgagcccgct cgttcgaaac gccagagagt ctcgagggcc 3060 tgtgatcagt gtcgagctgc acgtgaaaag tgtgatggaa tccagccggc ttgcttcccc 3120 tgtgtgtcgc agggccggtc gtgtacctac caggccagtc ccaagaagcg aggagtccag 3180 acgggctaca tccgcactct cgaactggct ctggcttgga tgttcgagaa cgttgcccgc 3240 agcgaggacg ccctccacaa tcttttggtc cgtgatgctg gccagggcag cgctctcctg 3300 gtcggcaaag actcgcctgc tgcagaacgc ctgcatgcaa gatgggcgac gagtcgagtc 3360 aacaaaagca tcacccgtct tctcgccact gcaagtatca tgccccatgt catggaacag 3420 cccctgtcaa cgtcaataaa tccggtcaat gatcggttta atggaatacc gaatcctacc 3480 ccctacaata gcgatgcggc cctagacgca attactcaaa ccaatgacta cggatcagtc 3540 aatacccatg gcattctcag tacatatcca ccacctgcca cccacttgaa cgaggcctcg 3600 gtggcccttg cgcctggagg tgctcctccc agaccacccc cgccatacgt tgatagtaca 3660 acgaaccatc ctccttacca tagtaacctc gtccccatgg ccaatttcgg atattcgact 3720 gtagactatg atgcgatggt ggatgatctg gcatcgatcg agtacacgga tgcggttgac 3780 gttgatccgc agttcatgac gaacctcggg tttgtcccag ggtgtaattt tagcgatatc 3840 aacacgtatg agcaaggtaa gcctatccct aaccctctcc ttggtctcga ttctacggcc 3900 cgagaaaatg aaatggatga aaacctagag caggtgagcg gcatcatcgg gaacctccgt 3960 cacatggccc tggatatggg caatgagatc gatacacaga atcgccagat cgacaggatc 4020 atggagaagg ctgattccaa caaaaccaga attgatgagg ccaaccaacg tgcaacaaag 4080 atgctgggaa gtggtgatga cgatgacaag caaatgacta aagacagatt agccgctctc 4140 catgccgccc aatccgatga cgaggaggag acggaggtgg ccgtcaatgt ggatggccat 4200 gattcctaca tggacgactt cttcgcccag gtggaggaga tccgcgggat gatcgacaag 4260 gtgcaggata acgtcgagga ggtaaagaag aagcactcgg ccatcctgtc cgccccacaa 4320 acggacgaga agaccaagca agagctggag gatctgatgg ccgacatcaa gaagaatgcc 4380 aatcgcgttc gcggcaagct caagggcatc gagcagaaca tcgagcagga agagcagcag 4440 aataagtcgt cggcggatct gagaattcgg aagacgcagc actcgacgtt gtcacggaaa 4500 ttcgtcgaag tgatgaccga gtacaatcgc acgcagaccg actaccgaga gcgctgcaag 4560 ggaaggatac agcgtcaact ggagattacc ggacgaccga ccaacgacga tgagctggag 4620 aagatgctgg aggagggcaa ctcgtctgtg ttcacgcagg gcatcatcat ggagacgcag 4680 caggccaaac agacgctggc ggacattgag gcccgccacc aggacatcat gaagctggag 4740 acatcgatca aggagctgca cgacatgttc atggacatgg ccatgctggt ggagtcgcag 4800 ggcgagatga tcgatcgcat tgagtaccat gtggagcacg ctatggacta tgtgcagacg 4860 gcaactcagg acaccaagaa ggcgctcaag taccagagta aagcccgacg aaagaagatc 4920 atgatactga tctgcctcac tgtgctgggc atcttagcgg cctcatatgt tagcagttat 4980 ttcatgtaac aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa 5040 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt 5100 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg 5160 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc 5220 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa 5280 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt 5340 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct 5400 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa 5460 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta 5520 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt 5580 gatgtatagt gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac 5640 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg 5700 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 5760 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 5820 atgtatctta tcatgtctgg atcccgttta aactacgcgt aattcaaaca gggttctggc 5880 gtcgttctcg tactgttttc cccaggccag tgctttagcg ttattgaaaa aggaagagta 5940 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg 6000 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac 6060 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg 6120 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc 6180 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg 6240 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat 6300 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg 6360 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg 6420 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc 6480 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt 6540 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct 6600 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc 6660 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca 6720 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct 6780 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt 6840 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 6900 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 6960 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 7020 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 7080 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 7140 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 7200 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 7260 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 7320 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 7380 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 7440 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 7500 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 7560 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 7620 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg 7680 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag 7740 a 7741 // ID MN658772; SV 1; linear; other DNA; STD; SYN; 8007 BP. XX AC MN658772; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct GST::BoNTA::GFPnb gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-8007 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; c321091e509a5acd3348c8a541c37d1a. XX FH Key Location/Qualifiers FH FT source 1..8007 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..941,3988..8007) FT /note="pGEX-kg" FT regulatory 185..213 FT /note="tac_promoter" FT /regulatory_class="promoter" FT CDS 259..3990 FT /codon_start=1 FT /transl_table=11 FT /product="GST::BoNTA::GFPnb" FT /protein_id="QGY73537.1" FT /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK FT FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY FT GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL FT YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLV FT PRGSPGMPFVNKQFNYKDPVNGVDIAYIKIPNAGQMQPVKAFKIHNKIWVIPERDTFTN FT PEEGDLNPPPEAKQVPVSYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIV FT RGIPFWGGSTIDTELKVIDTNCINVIQPDGSYRSEELNLVIIGPSADIIQFECKSFGHE FT VLNLTRNGYGSTQYIRFSPDFTFGFEESLEVDTNPLLGAGKFATDPAVTLAHELIHAGH FT RLYGIAINPNRVFKVNTNAYYEMSGLEVSFEELRTFGGHDAKFIDSLQENEFRLYYYNK FT FKDIASTLNKAKSIVGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEI FT YTEDNFVKFFKVLNRKTYLNFDKAVFKINIVPKVNYTIYDGFNLRNTNLAANFNGQNTE FT INNMNFTKLKNFTGLFEFYKLLCVRGIITSKTKSLDKGYNKAKSLVPRGSQALNDLCIK FT VNNWDLFFSPSEDNFTNDLNKGEEITSDTNIEAAEENISLDLIQQYYLTFNFDNEPENI FT SIENLSSDIIGQLELMPNIERFPNGKKYELDKYTMFHYLRAQEFEHGKSRIALTNSVNE FT ALLNPSRVYTFFSSDYVKKVNKATEAAMFLGWVEQLVYDFTDETSEVSTTDKIADITII FT IPYIGPALNIGNMLYKDDFVGALIFSGAVILLEFIPEIAIPVLGTFALVSYIANKVLTV FT QTIDNALSKRNEKWDEVYKYIVTNWLAKVNTQIDLIRKKMKEALENQAEATKAIINYQY FT NQYTEEEKNNINFNIDDLSSKLNESINKAMININKFLNQCSVSYLMNSMIPYGVKRLED FT FDASLKDALLKYIYDNRGTLIGQVDRLKDKVNNTLSTDIPFQLSKYVDNQRLLSTFTEY FT IKNIRSGMQVQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGM FT SSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQ FT VTVSSKSGGYPYDVPDYA" FT misc_feature 259..918 FT /note="GST" FT misc_feature 919..936 FT /note="thrombin site" FT misc_feature 943..2286 FT /note="BoNT/A1 light chain" FT misc_feature 2296..2313 FT /note="thrombin site" FT misc_feature 2320..3591 FT /note="BoNT/A1 translocation domain" FT misc_feature 3601..3951 FT /note="anti-GFP nanobody" FT misc_feature 3961..3987 FT /note="1xHA tag" XX SQ Sequence 8007 BP; 2261 A; 1713 C; 1916 G; 2117 T; 0 other; aacgttatcg actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt 60 ggtatggctg tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg 120 ttctggataa tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag 180 ctgttgacaa ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc 240 acacaggaaa cagtattcat gtcccctata ctaggttatt ggaaaattaa gggccttgtg 300 caacccactc gacttctttt ggaatatctt gaagaaaaat atgaagagca tttgtatgag 360 cgcgatgaag gtgataaatg gcgaaacaaa aagtttgaat tgggtttgga gtttcccaat 420 cttccttatt atattgatgg tgatgttaaa ttaacacagt ctatggccat catacgttat 480 atagctgaca agcacaacat gttgggtggt tgtccaaaag agcgtgcaga gatttcaatg 540 cttgaaggag cggttttgga tattagatac ggtgtttcga gaattgcata tagtaaagac 600 tttgaaactc tcaaagttga ttttcttagc aagctacctg aaatgctgaa aatgttcgaa 660 gatcgtttat gtcataaaac atatttaaat ggtgatcatg taacccatcc tgacttcatg 720 ttgtatgacg ctcttgatgt tgttttatac atggacccaa tgtgcctgga tgcgttccca 780 aaattagttt gttttaaaaa acgtattgaa gctatcccac aaattgataa gtacttgaaa 840 tccagcaagt atatagcatg gcctttgcag ggctggcaag ccacgtttgg tggtggcgac 900 catcctccaa aatcggatct ggttccgcgt ggatccccgg gtatgccatt tgttaataaa 960 caatttaatt ataaagatcc tgtaaatggt gttgatattg cttatataaa aattccaaat 1020 gcaggacaaa tgcaaccagt aaaagctttt aaaattcata ataaaatatg ggttattcca 1080 gaaagagata catttacaaa tcctgaagaa ggagatttaa atccaccacc agaagcaaaa 1140 caagttccag tttcatatta tgattcaaca tatttaagta cagataatga aaaagataat 1200 tatttaaagg gagttacaaa attatttgag agaatttatt caactgatct tggaagaatg 1260 ttgttaacat caatagtaag gggaatacca ttttggggtg gaagtacaat agatacagaa 1320 ttaaaagtta ttgatactaa ttgtattaat gtgatacaac cagatggtag ttatagatca 1380 gaagaactta atctagtaat aataggaccc tcagctgata ttatacagtt tgaatgtaaa 1440 agctttggac atgaagtttt gaatcttacg cgaaatggtt atggctctac tcaatacatt 1500 agatttagcc cagattttac atttggtttt gaggagtcac ttgaagttga tacaaatcct 1560 cttttaggtg caggcaaatt tgctacggat ccggcagtaa ctctggcaca cgagctcatc 1620 cacgctggtc accgtctgta cggtatcgcg attaatccaa atagggtttt taaagtaaat 1680 actaatgcct attatgaaat gagtgggtta gaagtaagct ttgaggaact tagaacattt 1740 gggggacatg atgcaaagtt tatagatagt ttacaggaaa acgaatttcg tctatattat 1800 tataataagt ttaaagatat agcaagtaca cttaataaag ctaaatcaat agtaggtact 1860 actgcttcat tacagtatat gaaaaatgtt tttaaagaga aatatctcct atctgaagat 1920 acatctggaa aattttcggt agataaatta aaatttgata agttatacaa aatgttaaca 1980 gagatttaca cagaggataa ttttgttaag ttttttaaag tacttaacag aaaaacatat 2040 ttgaattttg ataaagccgt atttaagata aatatagtac ctaaggtaaa ttacacaata 2100 tatgatggat ttaatttaag aaatacaaat ttagcagcaa actttaatgg tcaaaataca 2160 gaaattaata atatgaattt tactaaacta aaaaatttta ctggattgtt tgaattttat 2220 aagttgctat gtgtaagagg gataataact tctaaaacta aatcattaga taaaggatac 2280 aataaggcta aatctctggt accgcgtggt tctcaggcct tgaacgacct gtgtatcaaa 2340 gtcaataact gggacctgtt tttctcacct agtgaggata acttcaccaa cgacttaaac 2400 aaaggcgaag aaatcacctc agataccaat attgaggcag cggaagaaaa tattagcctg 2460 gatctgattc agcagtacta tctgaccttc aacttcgaca atgaacctga aaacattagc 2520 atcgagaatc tgtcgagtga tattattggc cagttagaac tgatgccgaa tattgaacgc 2580 tttccgaatg gcaaaaaata tgagctggat aagtacacga tgttccatta cttacgtgcc 2640 caagaatttg aacacggtaa aagccgtatt gcgctgacta atagtgtgaa tgaagcgctg 2700 ttaaatccgt cacgtgtgta tacgttcttt tcgtcggact atgtgaagaa agtgaacaaa 2760 gccactgaag cagcaatgtt tttaggttgg gtggaacaac tggtttatga ctttaccgat 2820 gaaacctcag aagtcagtac tacggacaaa attgccgata tcacgatcat tattccgtat 2880 attggtcctg ccttaaatat tggcaacatg ctgtacaaag acgattttgt tggtgcgctg 2940 atttttagtg gtgcagtgat tctgctggaa tttattccgg aaattgcgat tccggtttta 3000 ggcacttttg cactggttag ctatatcgcc aacaaagtat taacggtcca gaccattgat 3060 aatgcgttat cgaaacgcaa cgaaaaatgg gatgaggtgt acaaatacat cgtgaccaat 3120 tggttagcga aagtaaacac ccaaattgac ctgatccgca aaaagatgaa agaggcgtta 3180 gaaaatcagg cagaagcaac caaagcgatt atcaactacc agtataacca gtacaccgaa 3240 gaagagaaga acaacatcaa cttcaacatc gatgatctgt cgagcaaact gaatgaatcg 3300 atcaacaagg ccatgatcaa catcaacaag ttcctgaatc agtgctcagt aagctatctg 3360 atgaatagca tgattccgta tggcgtaaaa cgcttagagg attttgatgc gagcttaaaa 3420 gatgccctgc tgaaatacat ctacgataat cgcggcactt taattggcca agttgatcgt 3480 ctgaaggaca aagtgaacaa taccctgtca acggatattc cgtttcagct gagcaaatat 3540 gtcgataatc agcgtctgtt aagcaccttc acggagtata ttaagaacat tagatctgga 3600 atgcaagttc agctggtgga atcaggcggt gcgttggtac agccaggtgg ttccttacgc 3660 ctgagttgtg ccgcttctgg gtttccggtg aatcgctata gcatgcgctg gtatcggcaa 3720 gcacctggca aagaacgcga atgggtagcg ggtatgtcgt ctgcaggcga tcgttcctcg 3780 tatgaggact cagtcaaagg ccgtttcacc attagccgtg atgacgcgcg caataccgtc 3840 tatctgcaga tgaacagcct caaaccggaa gatacagccg tgtactactg caacgtcaac 3900 gttggctttg agtactgggg acaggggact caggttacgg tgagcagtaa gagtggtggt 3960 tacccatacg atgttcctga ctatgcgtaa cctcaagctt attcatcgtg actgactgac 4020 gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 4080 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 4140 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 4200 gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat 4260 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc 4320 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg 4380 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc 4440 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt 4500 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct 4560 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac 4620 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact 4680 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa 4740 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga 4800 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt 4860 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga 4920 agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg 4980 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat 5040 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat 5100 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc 5160 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga 5220 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc 5280 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag 5340 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc 5400 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 5460 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 5520 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 5580 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 5640 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 5700 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 5760 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 5820 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 5880 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 5940 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 6000 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 6060 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 6120 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 6180 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct 6240 tacgcatctg tgcggtattt cacaccgcat aaattccgac accatcgaat ggtgcaaaac 6300 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa 6360 accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg 6420 cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat 6480 ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt 6540 gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc 6600 gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag 6660 cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct 6720 gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa 6780 tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc 6840 ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat 6900 cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca 6960 taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc 7020 catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat 7080 gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct 7140 gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta 7200 tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga 7260 ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc 7320 actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt 7380 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc 7440 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc 7500 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct 7560 atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct 7620 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc 7680 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc 7740 tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct 7800 gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc 7860 tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg 7920 acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg 7980 cgaattattt ttgatggcgt tggaatt 8007 // ID MN658773; SV 1; linear; other DNA; STD; SYN; 12100 BP. XX AC MN658773; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct BONTA::GFPnb::CD2 gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-12100 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 4459224bbe01c921da4b0385014afec2. XX FH Key Location/Qualifiers FH FT source 1..12100 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7082,11344..12100) FT /note="pJFRC81" FT regulatory 6508..6602 FT /note="5xGal4_DBD" FT /regulatory_class="enhancer" FT regulatory 6636..6730 FT /note="5xGal4_DBD" FT /regulatory_class="enhancer" FT regulatory 6759..6986 FT /note="HS_promoter" FT /regulatory_class="promoter" FT misc_feature 7003..7080 FT /note="IVS" FT 5'UTR 7083..7103 FT /note="Syn21" FT CDS 7104..11342 FT /codon_start=1 FT /transl_table=11 FT /product="BONTA::GFPnb::CD2" FT /protein_id="QGY73538.1" FT /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTMPFVNKQFNYKDPVNG FT VDIAYIKIPNAGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLNPPPEAKQVPVSYYDS FT TYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGGSTIDTELKVIDTNC FT INVIQPDGSYRSEELNLVIIGPSADIIQFECKSFGHEVLNLTRNGYGSTQYIRFSPDFT FT FGFEESLEVDTNPLLGAGKFATDPAVTLAHELIHAGHRLYGIAINPNRVFKVNTNAYYE FT MSGLEVSFEELRTFGGHDAKFIDSLQENEFRLYYYNKFKDIASTLNKAKSIVGTTASLQ FT YMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVKFFKVLNRKTYLNFD FT KAVFKINIVPKVNYTIYDGFNLRNTNLAANFNGQNTEINNMNFTKLKNFTGLFEFYKLL FT CVRGIITSKTKSLDKGYNKAKSGSENLYFQGSGLVPRGSQALNDLCIKVNNWDLFFSPS FT EDNFTNDLNKGEEITSDTNIEAAEENISLDLIQQYYLTFNFDNEPENISIENLSSDIIG FT QLELMPNIERFPNGKKYELDKYTMFHYLRAQEFEHGKSRIALTNSVNEALLNPSRVYTF FT FSSDYVKKVNKATEAAMFLGWVEQLVYDFTDETSEVSTTDKIADITIIIPYIGPALNIG FT NMLYKDDFVGALIFSGAVILLEFIPEIAIPVLGTFALVSYIANKVLTVQTIDNALSKRN FT EKWDEVYKYIVTNWLAKVNTQIDLIRKKMKEALENQAEATKAIINYQYNQYTEEEKNNI FT NFNIDDLSSKLNESINKAMININKFLNQCSVSYLMNSMIPYGVKRLEDFDASLKDALLK FT YIYDNRGTLIGQVDRLKDKVNNTLSTDIPFQLSKYVDNQRLLSTFTEYIKNIRSGMQVQ FT LVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRSSYED FT SVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTVSSKSGGYP FT YDVPDYAGSENLYFQGGSLVPRGSGSDCRDSGTVWGALGHGINLNIPNFQMTDDIDEVR FT WERGSTLVAEFKRKMKPFLKSGAFEILANGDLKIKNLTRDDSGTYNVTVYSTNGTRILD FT KALDLRILEMVSKPMIYWECSNATLTCEVLEGTDVELKLYQGKEHLRSLRQKTMSYQWT FT NLRAPFKCKAVNRVSQESEMEVVNCPEKGLPLYLIVGVSAGGLLLVFFGALFIFCICKR FT KKRNRRRKGEELEIKASRMSTVERGPKPHSTQASAPASQNPVASQAPPPPGHHLQTPGH FT RPLPPSHRNREHQPKKRPPPSGTQVHQQKGPPLPRPRVQPKPPCGSGDVSLPPPNENAN FT SFCYENEVAL" FT misc_feature 7104..8540 FT /note="BoNT/A1 light chain" FT sig_peptide 7104..7190 FT /note="PAT3 signal peptide" FT misc_feature 8550..8570 FT /note="TEV site" FT misc_feature 8577..8594 FT /note="thrombin site" FT misc_feature 8601..9872 FT /note="BoNT/A1 translocation domain" FT misc_feature 9882..10232 FT /note="Anti-GFP nanobody" FT misc_feature 10242..10268 FT /note="1xHA tag" FT misc_feature 10275..10295 FT /note="TEV site" FT misc_feature 10302..10319 FT /note="thrombin site" FT misc_feature 10326..11297 FT /note="CD2 from rat" FT misc_feature 10875..10943 FT /note="transmembrane domain" FT misc_feature 11301..11339 FT /note="ER export signal from Kir2.1" FT 3'UTR 11343..12009 FT /note="P10" XX SQ Sequence 12100 BP; 3136 A; 3151 C; 2983 G; 2830 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct 6540 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg 6600 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt 6660 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact 6720 gtcctccgag cggagactct agcgctagcg acgtcgagcg ccggagtata aatagaggcg 6780 cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc gctaagcgaa 6840 agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta aagtgcaagt 6900 taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac tactgaaatc 6960 tgccaagaag taattattga atacaagaag agaactctga atagatctaa aaggtaggtt 7020 caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc tttacttcag 7080 gcaacttaaa aaaaaaaatc aaaatgcccc ccagcaccag cctgttgctg ctggccgccc 7140 tgctgccatt tgccctgccc gccagtgatt ggaagaccgg cgaagtgacc atgcccttcg 7200 tgaacaagca gttcaactac aaggatcccg tgaacggcgt ggacattgcc tacatcaaga 7260 tccccaacgc cggccagatg cagcccgtga aggcctttaa gatccacaac aagatctggg 7320 tcatccccga gcgcgatacc ttcaccaacc ccgaggaagg cgatctgaac cccccacccg 7380 aagccaagca ggtccccgtg tcctactacg atagcaccta cctgagcacc gataacgaga 7440 aggataacta cctgaagggc gtgaccaagc tgttcgagcg catctacagc accgatctgg 7500 gccgcatgct gctgacctcg atcgtgcgcg gcatcccgtt ctggggcggc agcaccatcg 7560 ataccgagct gaaggtcatc gataccaact gcatcaacgt gatccagccc gatggcagct 7620 accgcagcga ggaactgaac ctggtcatca tcggccccag cgccgatatc atccagttcg 7680 agtgcaagag cttcggccac gaggtgctga acctgacccg caacggctac ggcagcaccc 7740 agtacatccg cttcagcccc gatttcacct tcggcttcga ggaatccctg gaagtggata 7800 ccaaccccct gctgggagcc ggcaagttcg ccaccgatcc agccgtgacc ctggcccacg 7860 agctgattca tgccggccac cgcctgtacg gcattgccat caaccccaac cgcgtgttca 7920 aggtcaacac caacgcctac tacgagatga gcggcctgga agtgtccttc gaggaactgc 7980 gcaccttcgg cggacacgat gccaagttca tcgatagcct gcaggaaaac gagttccgcc 8040 tgtactacta caacaagttc aaggatatcg ccagcaccct gaacaaggcg aagagcatcg 8100 tgggcaccac cgccagcctg cagtacatga agaatgtgtt caaggaaaag tacctgctga 8160 gcgaggatac cagcggcaag ttctccgtgg ataagctgaa gttcgacaag ctgtacaaga 8220 tgctgaccga gatctacacc gaggacaact tcgtgaagtt cttcaaggtg ctgaatcgca 8280 agacctacct gaacttcgat aaggccgtgt tcaagattaa catcgtgccc aaggtcaact 8340 acaccatcta cgatggcttc aacctgcgca acaccaacct ggccgccaac ttcaacggcc 8400 agaacaccga gatcaacaac atgaacttca ccaagctgaa gaatttcacc ggcctgttcg 8460 agttctacaa gctgctgtgc gtgcgcggaa tcatcaccag caagaccaag agcctggata 8520 agggctacaa caaggccaag tccggcagcg agaacctgta ttttcaaggc agcggcctgg 8580 tgccacgcgg ctcccaggcc ctgaacgatc tgtgcattaa ggtcaacaac tgggacctgt 8640 tcttcagccc cagcgaggat aatttcacca acgatctgaa caagggcgag gaaatcacca 8700 gcgataccaa catcgaggcc gccgaggaga acatcagcct ggacctgatc cagcagtact 8760 acctgacctt caacttcgat aacgagcccg agaacatctc catcgagaac ctgagcagcg 8820 atatcatcgg ccagctggaa ctgatgccca acatcgagcg cttccccaac ggcaagaagt 8880 acgagctgga taagtacacc atgttccact acctgcgcgc ccaggaattc gagcacggca 8940 agagccgcat tgccctgacc aacagcgtga acgaggccct gctgaacccc agccgcgtgt 9000 acaccttctt cagcagcgat tacgtgaaga aggtcaacaa ggccaccgag gccgccatgt 9060 ttctgggctg ggtggaacag ctggtgtacg atttcaccga tgagacaagc gaggtgtcca 9120 ccaccgataa gatcgccgat atcaccatca tcatcccgta catcggccca gccctgaaca 9180 tcggcaacat gctgtacaag gatgatttcg tgggcgccct gatcttcagc ggagccgtga 9240 tcctgctgga attcatcccc gagatcgcca tccccgtgct gggcaccttc gccctggtgt 9300 cctacattgc caacaaggtg ctgaccgtgc agaccatcga taacgccctg agcaagcgca 9360 acgagaagtg ggatgaggtg tacaagtaca tcgtgaccaa ctggctggcc aaggtcaaca 9420 cccagatcga cctgatccgc aagaagatga aggaagccct ggaaaaccag gccgaggcca 9480 ccaaggccat catcaactac cagtacaatc agtacaccga ggaagagaag aacaacatca 9540 acttcaacat cgacgacctg tccagcaagc tgaacgagag cattaacaag gccatgatta 9600 acatcaacaa gtttctgaac cagtgcagcg tgtcctacct gatgaacagc atgatcccct 9660 acggcgtgaa gcgcctggaa gatttcgatg ccagcctgaa ggatgccctg ctgaagtaca 9720 tctacgataa ccgcggcacc ctgatcggcc aggtggaccg cctgaaggat aaggtcaaca 9780 ataccctgtc caccgatatc ccgttccagc tgtcgaagta cgtggacaac cagcgtctgc 9840 tgagcacctt caccgagtac atcaagaaca tccgcagcgg catgcaggtc cagctggtgg 9900 aaagtggcgg agccctggtg cagccaggtg gaagtctgcg actgagctgc gccgccagcg 9960 gcttccccgt gaatcgctac agcatgcgct ggtatcgcca ggccccaggc aaggaacgcg 10020 agtgggtggc cggaatgagc agtgccggcg atcgcagcag ctacgaggat agcgtcaagg 10080 gccgcttcac catcagccgc gacgatgccc gcaacaccgt gtacctgcag atgaacagcc 10140 tgaagcccga ggataccgcc gtgtactact gcaacgtgaa cgtgggcttc gagtactggg 10200 gccagggcac ccaggtcaca gtgtcctcca agagcggcgg ctacccctac gatgtgcccg 10260 attacgccgg cagcgagaac ctgtattttc aaggcggcag cctggtgcca cgcggcagtg 10320 gaagtgactg cagagacagt gggaccgtct ggggtgccct gggtcatggc atcaacctga 10380 acatccctaa ctttcaaatg actgatgata ttgatgaggt gcgatgggag agggggagca 10440 ccctggttgc cgagtttaaa aggaagatga agcctttttt gaaatcggga gcatttgaga 10500 tcttagcaaa tggagacttg aagataaaga atctgacaag agatgacagt ggcacctata 10560 atgtaacggt atacagcaca aatgggacac gtatcctgga caaggcactg gacttgagga 10620 tcctagagat ggtctcaaag ccgatgatct actgggagtg cagcaacgca accctgacct 10680 gtgaggtctt ggaaggaaca gatgttgaac taaagctgta ccaaggaaag gagcatctca 10740 ggagcctccg tcagaagacc atgagttacc agtggaccaa cctgagagca ccgtttaagt 10800 gcaaggcggt aaacagggtc agccaggagt ctgagatgga agttgtcaac tgtccagaga 10860 aaggtctgcc cctctatctc atagtggggg tcagtgcagg aggactcctc ttggtgttct 10920 ttggggcgct gtttattttc tgtatctgca agaggaaaaa acggaacagg aggagaaaag 10980 gtgaagagct ggagataaaa gcttccagaa tgagcactgt ggaaagggga cccaagccac 11040 actcaaccca ggcctcagca ccagcatcac agaatccagt ggcttcccaa gctcctcctc 11100 cacctggcca tcatctccag acacctggcc atcgtcccct gcctccaagc caccgtaacc 11160 gtgagcacca gccgaagaag agacctcctc catcaggcac acaggttcac cagcagaaag 11220 gccctccttt gcccagaccc cgagttcagc caaagcctcc ctgtgggagt ggagatgttt 11280 cgttgccacc ccctaatgag aacgcaaaca gcttttgcta tgaaaatgaa gttgccctct 11340 aaaatgaatc gtttttaaaa taacaaatca attgttttat aatattcgta cgattctttg 11400 attatgtaat aaaatgtgat cattaggaag attacgaaaa atataaaaaa tatgagttct 11460 gtgtgtataa caaatgctgt aaacgccaca attgtgtttg ttgcaaataa acccatgatt 11520 atttgattaa aattgttgtt ttctttgttc atagacaata gtgtgttttg cctaaacgtg 11580 tactgcataa actccatgcg agtgtatagc gagctagtgg ctaacgcttg ccccaccaaa 11640 gtagattcgt caaaatcctc aatttcatca ccctcctcca agtttaacat ttggccgtcg 11700 gaattaactt ctaaagatgc cacataatct aataaatgaa atagagattc aaacgtggcg 11760 tcatcgtccg tttcgaccat ttccgaaaag aactcgggca taaactctat gatttctctg 11820 gacgtggtgt tgtcgaaact ctcaaagtac gcagtcagga acgtgcgcga catgtcgtcg 11880 ggaaactcgc gcggaaacat gttgttgtaa ccgaacgggt cccatagcgc caaaaccaaa 11940 tctgccagcg tcaatagaat gagcacgatg ccgacaatgg agctggcttg gatagcgatt 12000 cgagttaacg gccggccgtt taaacgaatt cttgaagacg aaagggcctc gtgatacgcc 12060 tatttttata ggttaatgtc atgataataa tggtttctta 12100 // ID MN658774; SV 1; linear; other DNA; STD; SYN; 7000 BP. XX AC MN658774; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct BoNTA-LC gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-7000 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 156f0dc12e6d4191ef96afc73f5230d0. XX FH Key Location/Qualifiers FH FT source 1..7000 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..2674,4061..7000) FT /note="pAWH" FT regulatory 96..2649 FT /note="ActinC5" FT /regulatory_class="promoter" FT CDS 2697..4238 FT /codon_start=1 FT /transl_table=11 FT /product="BoNTA-LC" FT /protein_id="QGY73539.1" FT /translation="MPFVNKQFNYKDPVNGVDIAYIKIPNAGQMQPVKAFKIHNKIWVI FT PERDTFTNPEEGDLNPPPEAKQVPVSYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLG FT RMLLTSIVRGIPFWGGSTIDTELKVIDTNCINVIQPDGSYRSEELNLVIIGPSADIIQF FT ECKSFGHEVLNLTRNGYGSTQYIRFSPDFTFGFEESLEVDTNPLLGAGKFATDPAVTLA FT HELIHAGHRLYGIAINPNRVFKVNTNAYYEMSGLEVSFEELRTFGGHDAKFIDSLQENE FT FRLYYYNKFKDIASTLNKAKSIVGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDK FT LYKMLTEIYTEDNFVKFFKVLNRKTYLNFDKAVFKINIVPKVNYTIYDGFNLRNTNLAA FT NFNGQNTEINNMNFTKLKNFTGLFEFYKLLCVRGGGGSGGGGSALSENLYFQYPAFLYK FT VVSSATMDLHRGGGRIFYPYDVPDYAGYPYDVPDYAGSYPYDVPDYAAHGGHRST" FT misc_feature 2697..3995 FT /note="BoNT/A1 light chain" FT misc_feature 4032..4049 FT /note="TEV site" XX SQ Sequence 7000 BP; 1925 A; 1704 C; 1602 G; 1769 T; 0 other; gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact 60 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat 120 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca 180 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc 240 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat 300 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag 360 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc 420 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc 480 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg 540 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt 600 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc 660 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt 720 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt 780 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat 840 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt 900 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa 960 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg 1020 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg 1080 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc 1140 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc 1200 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt 1260 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact 1320 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc 1380 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat 1440 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg 1500 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca 1560 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt 1620 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag 1680 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct 1740 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt 1800 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca 1860 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc 1920 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct 1980 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta 2040 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa 2100 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat 2160 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac 2220 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac 2280 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga 2340 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt 2400 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg 2460 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc 2520 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc 2580 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa 2640 tccagagacc ccggatccag atatcgacaa gtttgtacaa aaaagcaggc tccaaaatgc 2700 ccttcgtgaa caagcagttc aactacaagg accccgtgaa cggcgtggac atcgcctaca 2760 tcaagatccc caacgccggc cagatgcagc ccgtgaaggc cttcaagatc cacaacaaga 2820 tctgggtgat ccccgagcgc gacaccttca ccaaccccga ggagggcgac ctgaaccccc 2880 cccccgaggc caagcaggtg cccgtgtcct actacgactc cacctacctg tccaccgaca 2940 acgagaagga caactacctg aagggcgtga ccaagctgtt cgagcgcatc tactccaccg 3000 acctgggccg catgctgctg acctccatcg tgcgcggcat ccccttctgg ggcggctcca 3060 ccatcgacac cgagctgaag gtgatcgaca ccaactgcat caacgtgatc cagcccgacg 3120 gctcctaccg ctccgaggag ctgaacctgg tgatcatcgg cccctccgcc gacatcatcc 3180 agttcgagtg caagtccttc ggccacgagg tgctgaacct gacccgcaac ggctacggct 3240 ccacccagta catccgcttc tcccccgact tcaccttcgg cttcgaggag tccctggagg 3300 tggacaccaa ccccctgctg ggcgccggca agttcgccac cgaccccgcc gtgaccctgg 3360 cccacgagct gatccacgcc ggccaccgcc tgtacggcat cgccatcaac cccaaccgcg 3420 tgttcaaggt gaacaccaac gcctactacg agatgtccgg cctggaggtg tccttcgagg 3480 agctgcgcac cttcggcggc cacgacgcca agttcatcga ctccctgcag gagaacgagt 3540 tccgcctgta ctactacaac aagttcaagg acatcgcctc caccctgaac aaggccaagt 3600 ccatcgtggg caccaccgcc tccctgcagt acatgaagaa cgtgttcaag gagaagtacc 3660 tgctgtccga ggacacctcc ggcaagttct ccgtggacaa gctgaagttc gacaagctgt 3720 acaagatgct gaccgagatc tacaccgagg acaacttcgt gaagttcttc aaggtgctga 3780 accgcaagac ctacctgaac ttcgacaagg ccgtgttcaa gatcaacatc gtgcccaagg 3840 tgaactacac catctacgac ggcttcaacc tgcgcaacac caacctggcc gccaacttca 3900 acggccagaa caccgagatc aacaacatga acttcaccaa gctgaagaac ttcaccggcc 3960 tgttcgagtt ctacaagctg ctgtgcgtgc gcggcggcgg cggaagtgga ggtggaggct 4020 cggcccttag tgagaacctg tacttccagt acccagcttt cttgtacaaa gtggtgagct 4080 ccgccaccat ggatctccac cgcggtggag gccgcatctt ttacccatac gatgttcctg 4140 actatgcggg ctatccctat gacgtcccgg actatgcagg atcctatcca tatgacgttc 4200 cagattacgc tgctcatggc ggacaccggt ccacgtgacg taagctagca ggatctttgt 4260 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag 4320 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt 4380 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt 4440 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc 4500 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt 4560 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg 4620 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa 4680 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct 4740 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt 4800 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag 4860 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac 4920 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg 4980 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt 5040 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga 5100 tcccgtttaa actacgcgta attcaaacag ggttctggcg tcgttctcgt actgttttcc 5160 ccaggccagt gctttagcgt tattgaaaaa ggaagagtat gagtattcaa catttccgtg 5220 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 5280 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 5340 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 5400 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 5460 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 5520 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 5580 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 5640 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 5700 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 5760 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 5820 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 5880 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 5940 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 6000 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 6060 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 6120 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 6180 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 6240 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 6300 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 6360 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 6420 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 6480 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 6540 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 6600 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 6660 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 6720 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 6780 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 6840 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 6900 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 6960 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 7000 // ID MN658775; SV 1; linear; other DNA; STD; SYN; 7498 BP. XX AC MN658775; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct TEVt173v::CD2 gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-7498 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; c624a97fcb14d9125992ce726826f1c7. XX FH Key Location/Qualifiers FH FT source 1..7498 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..2674,4559..7498) FT /note="pAWH" FT regulatory 96..2649 FT /note="ActinC5" FT /regulatory_class="promoter" FT CDS 2697..4547 FT /codon_start=1 FT /transl_table=11 FT /product="TEVt173v::CD2" FT /protein_id="QGY73540.1" FT /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTASHPTFLYKVGGHHHH FT HHHGESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLL FT VQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTN FT FQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFVN FT TNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQ FT LMNTTLYNKVGDCRDSGTVWGALGHGINLNIPNFQMTDDIDEVRWERGSTLVAEFKRKM FT KPFLKSGAFEILANGDLKIKNLTRDDSGTYNVTVYSTNGTRILDKALDLRILEMVSKPM FT IYWECSNATLTCEVLEGTDVELKLYQGKEHLRSLRQKTMSYQWTNLRAPFKCKAVNRVS FT QESEMEVVNCPEKGLPLYLIVGVSAGGLLLVFFGALFIFCICKRKKRNRRRKGEELEIK FT ASRMSTVERGPKPHSTQASAPASQNPVASQAPPPPGHHLQTPGHRPLPPSHRNREHQPK FT KRPPPSGTQVHQQKGPPLPRPRVQPKPPCGSGDVSLPPPN" FT misc_feature 2697..3548 FT /note="E. coli codon usage TEV T173V" FT sig_peptide 2697..2783 FT /note="PAT3 signal peptide" FT misc_feature 2820..2840 FT /note="polyhistidine" FT misc_feature 3357..3359 FT /note="T173V Mutation" FT misc_feature 3573..4544 FT /note="CD2 from rat" FT misc_feature 4122..4190 FT /note="transmembrane domain" XX SQ Sequence 7498 BP; 2148 A; 1659 C; 1719 G; 1972 T; 0 other; gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact 60 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat 120 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca 180 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc 240 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat 300 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag 360 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc 420 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc 480 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg 540 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt 600 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc 660 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt 720 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt 780 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat 840 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt 900 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa 960 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg 1020 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg 1080 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc 1140 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc 1200 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt 1260 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact 1320 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc 1380 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat 1440 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg 1500 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca 1560 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt 1620 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag 1680 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct 1740 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt 1800 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca 1860 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc 1920 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct 1980 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta 2040 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa 2100 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat 2160 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac 2220 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac 2280 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga 2340 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt 2400 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg 2460 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc 2520 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc 2580 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa 2640 tccagagacc ccggatccag atatcgacaa gtttgtacaa aaaagcaggc tacaaaatgc 2700 caccttcaac atcattgctg ctcctcgcag cacttcttcc attcgcttta ccagcaagcg 2760 attggaagac tggagaagtc actgctagcc acccaacttt tctatacaaa gttggaggtc 2820 atcatcatca tcatcatcat ggagaaagct tgtttaaggg gccgcgtgat tacaacccga 2880 tatcgagcac catttgtcat ttgacgaatg aatctgatgg gcacacaaca tcgttgtatg 2940 gtattggatt tggtcccttc atcattacaa acaagcactt gtttagaaga aataatggaa 3000 cactgttggt ccaatcacta catggtgtat tcaaggtcaa gaacaccacg actttgcaac 3060 aacacctcat tgatgggagg gacatgataa ttattcgcat gcctaaggat ttcccaccat 3120 ttcctcaaaa gctgaaattt agagagccac aaagggaaga gcgcatatgt cttgtgacaa 3180 ccaacttcca aactaagagc atgtctagca tggtgtcaga cactagttgc acattccctt 3240 catctgatgg catattctgg aagcattgga ttcaaaccaa ggatgggcag tgtggcagtc 3300 cattagtatc aactagagat gggttcattg ttggtataca ctcagcatcg aatttcgtga 3360 acacaaacaa ttatttcaca agcgtgccga aaaacttcat ggaattgttg acaaatcagg 3420 aggcgcagca gtgggttagt ggttggcgat taaatgctga ctcagtattg tgggggggcc 3480 ataaagtttt catggtgaaa cctgaagagc cttttcagcc agttaaggaa gcgactcaac 3540 tcatgaatac aactttgtat aataaagttg gagactgcag agacagtggg accgtctggg 3600 gtgccctggg tcatggcatc aacctgaaca tccctaactt tcaaatgact gatgatattg 3660 atgaggtgcg atgggagagg gggagcaccc tggttgccga gtttaaaagg aagatgaagc 3720 cttttttgaa atcgggagca tttgagatct tagcaaatgg agacttgaag ataaagaatc 3780 tgacaagaga tgacagtggc acctataatg taacggtata cagcacaaat gggacacgta 3840 tcctggacaa ggcactggac ttgaggatcc tagagatggt ctcaaagccg atgatctact 3900 gggagtgcag caacgcaacc ctgacctgtg aggtcttgga aggaacagat gttgaactaa 3960 agctgtacca aggaaaggag catctcagga gcctccgtca gaagaccatg agttaccagt 4020 ggaccaacct gagagcaccg tttaagtgca aggcggtaaa cagggtcagc caggagtctg 4080 agatggaagt tgtcaactgt ccagagaaag gtctgcccct ctatctcata gtgggggtca 4140 gtgcaggagg actcctcttg gtgttctttg gggcgctgtt tattttctgt atctgcaaga 4200 ggaaaaaacg gaacaggagg agaaaaggtg aagagctgga gataaaagct tccagaatga 4260 gcactgtgga aaggggaccc aagccacact caacccaggc ctcagcacca gcatcacaga 4320 atccagtggc ttcccaagct cctcctccac ctggccatca tctccagaca cctggccatc 4380 gtcccctgcc tccaagccac cgtaaccgtg agcaccagcc gaagaagaga cctcctccat 4440 caggcacaca ggttcaccag cagaaaggcc ctcctttgcc cagaccccga gttcagccaa 4500 agcctccctg tgggagtgga gatgtttcgt tgccaccccc taattaatac ccagctttct 4560 tgtacaaagt ggtgagctcc gccaccatgg atctccaccg cggtggaggc cgcatctttt 4620 acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat 4680 cctatccata tgacgttcca gattacgctg ctcatggcgg acaccggtcc acgtgacgta 4740 agctagcagg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact 4800 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa 4860 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga 4920 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag 4980 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt 5040 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag 5100 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata 5160 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca 5220 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc 5280 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat 5340 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg 5400 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 5460 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 5520 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 5580 tatcttatca tgtctggatc ccgtttaaac tacgcgtaat tcaaacaggg ttctggcgtc 5640 gttctcgtac tgttttcccc aggccagtgc tttagcgtta ttgaaaaagg aagagtatga 5700 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 5760 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 5820 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 5880 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 5940 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 6000 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 6060 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 6120 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 6180 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 6240 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 6300 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 6360 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 6420 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 6480 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 6540 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 6600 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 6660 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 6720 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 6780 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 6840 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 6900 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6960 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 7020 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 7080 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 7140 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 7200 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 7260 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 7320 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 7380 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 7440 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaga 7498 // ID MN658776; SV 1; linear; other DNA; STD; SYN; 10555 BP. XX AC MN658776; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct 3xHalo7::CAAX gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-10555 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 05ee647f19c6a1c5d2c3a3844e64deae. XX FH Key Location/Qualifiers FH FT source 1..10555 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..6463,10465..10555) FT /note="pJFRC19" FT misc_feature 6464..6898 FT /note="pQUAST" FT regulatory 6515..6608 FT /note="5XQUAS" FT /regulatory_class="enhancer" FT regulatory 6636..6898 FT /note="TATA" FT /regulatory_class="TATA_box" FT misc_feature 6899..6976 FT /note="IVS" FT 5'UTR 6985..7005 FT /note="L21" FT CDS 7006..9792 FT /codon_start=1 FT /transl_table=11 FT /product="3xHalo7::CAAX" FT /protein_id="QGY73541.1" FT /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS FT YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI FT HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII FT DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV FT EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL FT IGSEIARWLSTLEISGRSTRGSMAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLF FT LHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALG FT LEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTT FT DVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGE FT PANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNL FT LQEDNPDLIGSEIARWLSTLEISGRSTRGSMAEIGTGFPFDPHYVEVLGERMHYVDVGP FT RDGTPVLFLHGNPTSSYVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFM FT DAFIEALGLEEVVLVIHDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARE FT TFQAFRTTDVGRKLIIDQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFP FT NELPIAGEPANIVALVEEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAV FT DIGPGLNLLQEDNPDLIGSEIARWLSTLEISGRSTSLEMSKDGKKKKKKSKTKCVIM" FT misc_feature 7006..7896 FT /note="Halo7" FT misc_feature 7915..8805 FT /note="Halo7" FT misc_feature 8824..9714 FT /note="Halo7" FT misc_feature 9715..9792 FT /note="Linker::CAAX" FT misc_feature 9778..9792 FT /note="CAAX-motif" FT 3'UTR 9793..10464 FT /note="P10" XX SQ Sequence 10555 BP; 2573 A; 2745 C; 2589 G; 2648 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcgcttggc tgcatccaac 6480 gcgttgggag ctctccggat ccaagcttgg atccgggtaa tcgcttatcc tcggataaac 6540 aattatcctc acgggtaatc gcttatccgc tcgggtaatc gcttatcctc gggtaatcgc 6600 ttatccttaa gcttgatatc gaattgctgc agcccgagcg gagactctag cgagcgccgg 6660 agtataaata gaggcgcttc gtctacggag cgacaattca attcaaacaa gcaaagtgaa 6720 cacgtcgcta agcgaaagct aagcaaataa acaagcgcag ctgaacaagc taaacaatct 6780 gcagtaaagt gcaagttaaa gtgaatcaat taaaagtaac cagcaaccaa gtaaatcaac 6840 tgcaactact gaaatctgcc aagaagtaat tattgaatac aagaagagaa ctctgaatag 6900 atctaaaagg taggttcaac cactgatgcc taggcacacc gaaacgacta accctaattc 6960 ttatccttta cttcaggcgg ccgcaactcc taaaaaaccg ccaccatggc agaaatcggt 7020 actggctttc cattcgaccc ccattatgtg gaagtcctgg gcgagcgcat gcactacgtc 7080 gatgttggtc cgcgcgatgg cacccctgtg ctgttcctgc acggtaaccc gacctcctcc 7140 tacgtgtggc gcaacatcat cccgcatgtt gcaccgaccc atcgctgcat tgctccagac 7200 ctgatcggta tgggcaaatc cgacaaacca gacctgggtt atttcttcga cgaccacgtc 7260 cgcttcatgg atgccttcat cgaagccctg ggtctggaag aggtcgtcct ggtcattcac 7320 gactggggct ccgctctggg tttccactgg gccaagcgca atccagagcg cgtcaaaggt 7380 attgcattta tggagttcat ccgccctatc ccgacctggg acgaatggcc agaatttgcc 7440 cgcgagacct tccaggcctt ccgcaccacc gacgtcggcc gcaagctgat catcgatcag 7500 aacgttttta tcgagggtac gctgccgatg ggtgtcgtcc gcccgctgac tgaagtcgag 7560 atggaccatt accgcgagcc gttcctgaat cctgttgacc gcgagccact gtggcgcttc 7620 ccaaacgagc tgccaatcgc cggtgagcca gcgaacatcg tcgcgctggt cgaagaatac 7680 atggactggc tgcaccagtc ccctgtcccg aagctgctgt tctggggcac cccaggcgtt 7740 ctgatcccac cggccgaagc cgctcgcctg gccaaaagcc tgcctaactg caaggctgtg 7800 gacatcggcc cgggtctgaa tctgctgcaa gaagacaacc cggacctgat cggcagcgag 7860 atcgcgcgct ggctgtcgac gctcgaaatt tccggcagat ctactagagg atccatggca 7920 gaaatcggta ctggctttcc attcgacccc cattatgtgg aagtcctggg cgagcgcatg 7980 cactacgtcg atgttggtcc gcgcgatggc acccctgtgc tgttcctgca cggtaacccg 8040 acctcctcct acgtgtggcg caacatcatc ccgcatgttg caccgaccca tcgctgcatt 8100 gctccagacc tgatcggtat gggcaaatcc gacaaaccag acctgggtta tttcttcgac 8160 gaccacgtcc gcttcatgga tgccttcatc gaagccctgg gtctggaaga ggtcgtcctg 8220 gtcattcacg actggggctc cgctctgggt ttccactggg ccaagcgcaa tccagagcgc 8280 gtcaaaggta ttgcatttat ggagttcatc cgccctatcc cgacctggga cgaatggcca 8340 gaatttgccc gcgagacctt ccaggccttc cgcaccaccg acgtcggccg caagctgatc 8400 atcgatcaga acgtttttat cgagggtacg ctgccgatgg gtgtcgtccg cccgctgact 8460 gaagtcgaga tggaccatta ccgcgagccg ttcctgaatc ctgttgaccg cgagccactg 8520 tggcgcttcc caaacgagct gccaatcgcc ggtgagccag cgaacatcgt cgcgctggtc 8580 gaagaataca tggactggct gcaccagtcc cctgtcccga agctgctgtt ctggggcacc 8640 ccaggcgttc tgatcccacc ggccgaagcc gctcgcctgg ccaaaagcct gcctaactgc 8700 aaggctgtgg acatcggccc gggtctgaat ctgctgcaag aagacaaccc ggacctgatc 8760 ggcagcgaga tcgcgcgctg gctgtcgacg ctcgaaattt ccggcagatc tactagagga 8820 tccatggcag aaatcggtac tggctttcca ttcgaccccc attatgtgga agtcctgggc 8880 gagcgcatgc actacgtcga tgttggtccg cgcgatggca cccctgtgct gttcctgcac 8940 ggtaacccga cctcctccta cgtgtggcgc aacatcatcc cgcatgttgc accgacccat 9000 cgctgcattg ctccagacct gatcggtatg ggcaaatccg acaaaccaga cctgggttat 9060 ttcttcgacg accacgtccg cttcatggat gccttcatcg aagccctggg tctggaagag 9120 gtcgtcctgg tcattcacga ctggggctcc gctctgggtt tccactgggc caagcgcaat 9180 ccagagcgcg tcaaaggtat tgcatttatg gagttcatcc gccctatccc gacctgggac 9240 gaatggccag aatttgcccg cgagaccttc caggccttcc gcaccaccga cgtcggccgc 9300 aagctgatca tcgatcagaa cgtttttatc gagggtacgc tgccgatggg tgtcgtccgc 9360 ccgctgactg aagtcgagat ggaccattac cgcgagccgt tcctgaatcc tgttgaccgc 9420 gagccactgt ggcgcttccc aaacgagctg ccaatcgccg gtgagccagc gaacatcgtc 9480 gcgctggtcg aagaatacat ggactggctg caccagtccc ctgtcccgaa gctgctgttc 9540 tggggcaccc caggcgttct gatcccaccg gccgaagccg ctcgcctggc caaaagcctg 9600 cctaactgca aggctgtgga catcggcccg ggtctgaatc tgctgcaaga agacaacccg 9660 gacctgatcg gcagcgagat cgcgcgctgg ctgtcgacgc tcgaaatttc cggcagatct 9720 actagtctcg agatgagcaa agatggtaaa aagaagaaaa agaagtcaaa gacaaagtgt 9780 gtaattatgt aatctagaat gaatcgtttt taaaataaca aatcaattgt tttataatat 9840 tcgtacgatt ctttgattat gtaataaaat gtgatcatta ggaagattac gaaaaatata 9900 aaaaatatga gttctgtgtg tataacaaat gctgtaaacg ccacaattgt gtttgttgca 9960 aataaaccca tgattatttg attaaaattg ttgttttctt tgttcataga caatagtgtg 10020 ttttgcctaa acgtgtactg cataaactcc atgcgagtgt atagcgagct agtggctaac 10080 gcttgcccca ccaaagtaga ttcgtcaaaa tcctcaattt catcaccctc ctccaagttt 10140 aacatttggc cgtcggaatt aacttctaaa gatgccacat aatctaataa atgaaataga 10200 gattcaaacg tggcgtcatc gtccgtttcg accatttccg aaaagaactc gggcataaac 10260 tctatgattt ctctggacgt ggtgttgtcg aaactctcaa agtacgcagt caggaacgtg 10320 cgcgacatgt cgtcgggaaa ctcgcgcgga aacatgttgt tgtaaccgaa cgggtcccat 10380 agcgccaaaa ccaaatctgc cagcgtcaat agaatgagca cgatgccgac aatggagctg 10440 gcttggatag cgattcgagt taacggccgg ccgtttaaac gaattcttga agacgaaagg 10500 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tctta 10555 // ID MN658777; SV 1; linear; other DNA; STD; SYN; 14887 BP. XX AC MN658777; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct B2 recombinase and PAT3SP::BoNTA::GFPnb::CD2 genes, DE complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-14887 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 745e23a1c3e1b2f83513575d66a52d48. XX FH Key Location/Qualifiers FH FT source 1..14887 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7332,14148..14887) FT /note="pJFRC161" FT regulatory 6508..6602 FT /note="5xGal4_DBD" FT /regulatory_class="enhancer" FT regulatory 6636..6730 FT /note="5xGal4_DBD" FT /regulatory_class="enhancer" FT regulatory 6783..6910 FT /note="5xGal4_DBD" FT /regulatory_class="enhancer" FT regulatory 6911..6982 FT /note="5xGal4_DBD" FT /regulatory_class="enhancer" FT regulatory 7009..7236 FT /note="HS promoter" FT /regulatory_class="promoter" FT misc_feature 7261..7332 FT /note="IVS" FT misc_feature 7333..7365 FT /note="B3RT" FT 5'UTR 7366..7386 FT /note="Syn21" FT CDS 7387..8811 FT /codon_start=1 FT /transl_table=11 FT /product="B2 recombinase" FT /protein_id="QGY73543.1" FT /translation="MSEFSELVRILPLDQVAEIKRILSRGDPIPLQRLASLLTMVILTV FT NMSKKRKSSPIKLSTFTKYRRNVAKSLYYDMSSKTVFFEYHLKNTQDLQEGLEQAIAPY FT NFVVKVHKKPIDWQKQLSSVHERKAGHRSILSNNVGAEISKLAETKDSTWSFIERTMDL FT IEARTRQPTTRVAYRFLLQLTFMNCCRANDLKNADPSTFQIIADPHLGRILRAFVPETK FT TSIERFIYFFPCKGRCDPLLALDSYLLWVGPVPKTQTTDEETQYDYQLLQDTLLISYDR FT FIAKESKENIFKIPNGPKAHLGRHLMASYLGNNSLKSEATLYGNWSVERQEGVSKMADS FT RYMHTVKKSPPSYLFAFLSGYYKKSNQGEYVLAETLYNPLDYDKTLPITTNEKLICRRY FT GKNAKVIPKDALLYLYTYAQQKRKQLADPNEQNRLFSSESPAHPFLTPQSTGSSTPLTW FT TAPKTLSTGLMTPGEE" FT 3'UTR 8817..9059 FT /note="Hsp70B" FT 3'UTR 9138..9834 FT /note="SV40" FT misc_feature 9836..9868 FT /note="B3RT" FT 5'UTR 9887..9907 FT /note="Syn21" FT CDS 9908..14146 FT /codon_start=1 FT /transl_table=11 FT /product="PAT3SP::BoNTA::GFPnb::CD2" FT /protein_id="QGY73542.1" FT /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTMPFVNKQFNYKDPVNG FT VDIAYIKIPNAGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLNPPPEAKQVPVSYYDS FT TYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGGSTIDTELKVIDTNC FT INVIQPDGSYRSEELNLVIIGPSADIIQFECKSFGHEVLNLTRNGYGSTQYIRFSPDFT FT FGFEESLEVDTNPLLGAGKFATDPAVTLAHELIHAGHRLYGIAINPNRVFKVNTNAYYE FT MSGLEVSFEELRTFGGHDAKFIDSLQENEFRLYYYNKFKDIASTLNKAKSIVGTTASLQ FT YMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVKFFKVLNRKTYLNFD FT KAVFKINIVPKVNYTIYDGFNLRNTNLAANFNGQNTEINNMNFTKLKNFTGLFEFYKLL FT CVRGIITSKTKSLDKGYNKAKSGSENLYFQGSGLVPRGSQALNDLCIKVNNWDLFFSPS FT EDNFTNDLNKGEEITSDTNIEAAEENISLDLIQQYYLTFNFDNEPENISIENLSSDIIG FT QLELMPNIERFPNGKKYELDKYTMFHYLRAQEFEHGKSRIALTNSVNEALLNPSRVYTF FT FSSDYVKKVNKATEAAMFLGWVEQLVYDFTDETSEVSTTDKIADITIIIPYIGPALNIG FT NMLYKDDFVGALIFSGAVILLEFIPEIAIPVLGTFALVSYIANKVLTVQTIDNALSKRN FT EKWDEVYKYIVTNWLAKVNTQIDLIRKKMKEALENQAEATKAIINYQYNQYTEEEKNNI FT NFNIDDLSSKLNESINKAMININKFLNQCSVSYLMNSMIPYGVKRLEDFDASLKDALLK FT YIYDNRGTLIGQVDRLKDKVNNTLSTDIPFQLSKYVDNQRLLSTFTEYIKNIRSGMQVQ FT LVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRSSYED FT SVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTVSSKSGGYP FT YDVPDYAGSENLYFQGGSLVPRGSGSDCRDSGTVWGALGHGINLNIPNFQMTDDIDEVR FT WERGSTLVAEFKRKMKPFLKSGAFEILANGDLKIKNLTRDDSGTYNVTVYSTNGTRILD FT KALDLRILEMVSKPMIYWECSNATLTCEVLEGTDVELKLYQGKEHLRSLRQKTMSYQWT FT NLRAPFKCKAVNRVSQESEMEVVNCPEKGLPLYLIVGVSAGGLLLVFFGALFIFCICKR FT KKRNRRRKGEELEIKASRMSTVERGPKPHSTQASAPASQNPVASQAPPPPGHHLQTPGH FT RPLPPSHRNREHQPKKRPPPSGTQVHQQKGPPLPRPRVQPKPPCGSGDVSLPPPNENAN FT SFCYENEVAL" FT misc_feature 9908..11344 FT /note="BoNT/A1 light chain" FT sig_peptide 9908..9994 FT /note="PAT3 signal peptide" FT misc_feature 11354..11374 FT /note="TEV site" FT misc_feature 11381..11398 FT /note="thrombin site" FT misc_feature 11405..12676 FT /note="BoNT/A1 translocation domain" FT misc_feature 12686..13036 FT /note="Anti-GFP nanobody" FT misc_feature 13046..13072 FT /note="1xHA tag" FT misc_feature 13079..13099 FT /note="TEV site" FT misc_feature 13106..13123 FT /note="thrombin site" FT misc_feature 13130..14101 FT /note="CD2 from rat" FT misc_feature 13679..13747 FT /note="transmembrane domain" FT misc_feature 14105..14143 FT /note="ER export signal from Kir2.1" FT 3'UTR 14148..14813 FT /note="P10" XX SQ Sequence 14887 BP; 3920 A; 3811 C; 3601 G; 3555 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct 6540 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg 6600 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt 6660 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact 6720 gtcctccgag cggagactct agcgctagcg catgcctgca ggtcggagta ctgtcctccg 6780 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc 6840 ggagtactgt cctccgagcg gagactctag cactagtgca tgcctgcagg tcggagtact 6900 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc 6960 ctccgagcgg agtactgtcc tccgagcgga gactctagcg acgtcgagcg ccggagtata 7020 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc 7080 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta 7140 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac 7200 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagatctaa 7260 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc 7320 tttacttcag gcggttgctt aagaataagt aattcttaag caaccaactt aaaaaaaaaa 7380 atcaaaatgt cggaatttag tgagttggta cgtatcttgc ctttggatca ggttgcggag 7440 atcaaacgta ttctgagccg tggcgatccc atcccactcc agcgcctggc ttcgctgctg 7500 accatggtga ttttgacggt gaacatgtcc aaaaagcgca aatcgtcccc tataaaactg 7560 tcgaccttca ccaagtaccg acgcaacgtg gctaagtcgt tgtactacga tatgtcgtcc 7620 aagacagtgt tctttgagta ccacttgaag aacactcagg acctgcagga aggcctggag 7680 caggccatcg ctccgtacaa cttcgtagtg aaggtgcata agaaacccat agactggcag 7740 aaacaactta gtagcgtcca cgaacgcaag gcaggccacc gttcgatcct gtccaataat 7800 gtgggcgccg agatttccaa gctggcagag accaaagata gcacttggtc gttcatcgaa 7860 cgaaccatgg acctgatcga ggctcgcacc cgtcagccca ccacccgggt tgcatatcgg 7920 tttctgctgc agctgacgtt catgaactgt tgccgtgcga atgacttgaa gaacgccgat 7980 cccagcacgt tccagatcat tgctgatccc cacctgggtc gtatcctccg cgcctttgtg 8040 cccgagacaa agaccagtat cgagcggttt atctacttct tcccatgtaa aggccgctgc 8100 gatccactcc ttgccttgga cagttacctg ttgtgggtgg gcccggtgcc caagacgcag 8160 accacggatg aagagacgca atacgactat cagctgttgc aagatacact ccttatcagc 8220 tacgatcgct tcatagccaa agaaagtaag gaaaatatct tcaagattcc caacggaccg 8280 aaggctcacc tgggccgcca cctcatggcc tcgtaccttg gaaataattc gcttaagtcg 8340 gaggctacgc tgtacggtaa ctggtcggtg gaacggcaag agggagtttc gaaaatggcc 8400 gacagccgat acatgcacac cgttaagaaa agccctcctt cctacctctt cgcctttttg 8460 tcgggttatt acaagaagag taaccaaggc gaatacgtgc ttgcagaaac cctctacaac 8520 cccttggatt acgataaaac actgccaata actaccaatg agaagttgat ctgtcgccgg 8580 tatggtaaga acgcgaaggt gatccccaaa gatgccctgt tgtatctgta cacctatgct 8640 cagcagaagc gtaaacagct tgccgatccg aatgaacaga atcgcctgtt ttcgagcgaa 8700 tccccggcac accccttcct gactccccag tcgacgggca gctcgacgcc gctgacctgg 8760 accgcaccga agacgctttc caccggcctg atgacaccgg gcgaagagta agaattgact 8820 aaagccaaat agaaaattat tcagttcctg gcttaagttt ttaaaagtga tattatttat 8880 ttggttgtaa ccaaccaaaa gaatgtaaat aactaataca taattatgtt agttttaagt 8940 tagcaacaaa ttgattttag ctatattagc tacttggtta ataaatagaa tatatttatt 9000 taaagataat tgcgttttta ttgtcaggga gtgagtttgc ttaaaaactc gtttagatcc 9060 actagttcta gttgcggccg gctaagaatt cgatatcaag cttatcgata ccgtcgacct 9120 cgagggtacc tctagaggat ctttgtgaag gaaccttact tctgtggtgt gacataattg 9180 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa 9240 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat 9300 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg 9360 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag 9420 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct 9480 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca 9540 ctgctataca agaaaattat ggaaaaatat ttgatgtata gtgccttgac tagagatcat 9600 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 9660 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 9720 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 9780 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcggttg 9840 cttaagaata agtaattctt aagcaaccca aatccgccac gaatcaaact taaaaaaaaa 9900 aatcaaaatg ccccccagca ccagcctgtt gctgctggcc gccctgctgc catttgccct 9960 gcccgccagt gattggaaga ccggcgaagt gaccatgccc ttcgtgaaca agcagttcaa 10020 ctacaaggat cccgtgaacg gcgtggacat tgcctacatc aagatcccca acgccggcca 10080 gatgcagccc gtgaaggcct ttaagatcca caacaagatc tgggtcatcc ccgagcgcga 10140 taccttcacc aaccccgagg aaggcgatct gaacccccca cccgaagcca agcaggtccc 10200 cgtgtcctac tacgatagca cctacctgag caccgataac gagaaggata actacctgaa 10260 gggcgtgacc aagctgttcg agcgcatcta cagcaccgat ctgggccgca tgctgctgac 10320 ctcgatcgtg cgcggcatcc cgttctgggg cggcagcacc atcgataccg agctgaaggt 10380 catcgatacc aactgcatca acgtgatcca gcccgatggc agctaccgca gcgaggaact 10440 gaacctggtc atcatcggcc ccagcgccga tatcatccag ttcgagtgca agagcttcgg 10500 ccacgaggtg ctgaacctga cccgcaacgg ctacggcagc acccagtaca tccgcttcag 10560 ccccgatttc accttcggct tcgaggaatc cctggaagtg gataccaacc ccctgctggg 10620 agccggcaag ttcgccaccg atccagccgt gaccctggcc cacgagctga ttcatgccgg 10680 ccaccgcctg tacggcattg ccatcaaccc caaccgcgtg ttcaaggtca acaccaacgc 10740 ctactacgag atgagcggcc tggaagtgtc cttcgaggaa ctgcgcacct tcggcggaca 10800 cgatgccaag ttcatcgata gcctgcagga aaacgagttc cgcctgtact actacaacaa 10860 gttcaaggat atcgccagca ccctgaacaa ggcgaagagc atcgtgggca ccaccgccag 10920 cctgcagtac atgaagaatg tgttcaagga aaagtacctg ctgagcgagg ataccagcgg 10980 caagttctcc gtggataagc tgaagttcga caagctgtac aagatgctga ccgagatcta 11040 caccgaggac aacttcgtga agttcttcaa ggtgctgaat cgcaagacct acctgaactt 11100 cgataaggcc gtgttcaaga ttaacatcgt gcccaaggtc aactacacca tctacgatgg 11160 cttcaacctg cgcaacacca acctggccgc caacttcaac ggccagaaca ccgagatcaa 11220 caacatgaac ttcaccaagc tgaagaattt caccggcctg ttcgagttct acaagctgct 11280 gtgcgtgcgc ggaatcatca ccagcaagac caagagcctg gataagggct acaacaaggc 11340 caagtccggc agcgagaacc tgtattttca aggcagcggc ctggtgccac gcggctccca 11400 ggccctgaac gatctgtgca ttaaggtcaa caactgggac ctgttcttca gccccagcga 11460 ggataatttc accaacgatc tgaacaaggg cgaggaaatc accagcgata ccaacatcga 11520 ggccgccgag gagaacatca gcctggacct gatccagcag tactacctga ccttcaactt 11580 cgataacgag cccgagaaca tctccatcga gaacctgagc agcgatatca tcggccagct 11640 ggaactgatg cccaacatcg agcgcttccc caacggcaag aagtacgagc tggataagta 11700 caccatgttc cactacctgc gcgcccagga attcgagcac ggcaagagcc gcattgccct 11760 gaccaacagc gtgaacgagg ccctgctgaa ccccagccgc gtgtacacct tcttcagcag 11820 cgattacgtg aagaaggtca acaaggccac cgaggccgcc atgtttctgg gctgggtgga 11880 acagctggtg tacgatttca ccgatgagac aagcgaggtg tccaccaccg ataagatcgc 11940 cgatatcacc atcatcatcc cgtacatcgg cccagccctg aacatcggca acatgctgta 12000 caaggatgat ttcgtgggcg ccctgatctt cagcggagcc gtgatcctgc tggaattcat 12060 ccccgagatc gccatccccg tgctgggcac cttcgccctg gtgtcctaca ttgccaacaa 12120 ggtgctgacc gtgcagacca tcgataacgc cctgagcaag cgcaacgaga agtgggatga 12180 ggtgtacaag tacatcgtga ccaactggct ggccaaggtc aacacccaga tcgacctgat 12240 ccgcaagaag atgaaggaag ccctggaaaa ccaggccgag gccaccaagg ccatcatcaa 12300 ctaccagtac aatcagtaca ccgaggaaga gaagaacaac atcaacttca acatcgacga 12360 cctgtccagc aagctgaacg agagcattaa caaggccatg attaacatca acaagtttct 12420 gaaccagtgc agcgtgtcct acctgatgaa cagcatgatc ccctacggcg tgaagcgcct 12480 ggaagatttc gatgccagcc tgaaggatgc cctgctgaag tacatctacg ataaccgcgg 12540 caccctgatc ggccaggtgg accgcctgaa ggataaggtc aacaataccc tgtccaccga 12600 tatcccgttc cagctgtcga agtacgtgga caaccagcgt ctgctgagca ccttcaccga 12660 gtacatcaag aacatccgca gcggcatgca ggtccagctg gtggaaagtg gcggagccct 12720 ggtgcagcca ggtggaagtc tgcgactgag ctgcgccgcc agcggcttcc ccgtgaatcg 12780 ctacagcatg cgctggtatc gccaggcccc aggcaaggaa cgcgagtggg tggccggaat 12840 gagcagtgcc ggcgatcgca gcagctacga ggatagcgtc aagggccgct tcaccatcag 12900 ccgcgacgat gcccgcaaca ccgtgtacct gcagatgaac agcctgaagc ccgaggatac 12960 cgccgtgtac tactgcaacg tgaacgtggg cttcgagtac tggggccagg gcacccaggt 13020 cacagtgtcc tccaagagcg gcggctaccc ctacgatgtg cccgattacg ccggcagcga 13080 gaacctgtat tttcaaggcg gcagcctggt gccacgcggc agtggaagtg actgcagaga 13140 cagtgggacc gtctggggtg ccctgggtca tggcatcaac ctgaacatcc ctaactttca 13200 aatgactgat gatattgatg aggtgcgatg ggagaggggg agcaccctgg ttgccgagtt 13260 taaaaggaag atgaagcctt ttttgaaatc gggagcattt gagatcttag caaatggaga 13320 cttgaagata aagaatctga caagagatga cagtggcacc tataatgtaa cggtatacag 13380 cacaaatggg acacgtatcc tggacaaggc actggacttg aggatcctag agatggtctc 13440 aaagccgatg atctactggg agtgcagcaa cgcaaccctg acctgtgagg tcttggaagg 13500 aacagatgtt gaactaaagc tgtaccaagg aaaggagcat ctcaggagcc tccgtcagaa 13560 gaccatgagt taccagtgga ccaacctgag agcaccgttt aagtgcaagg cggtaaacag 13620 ggtcagccag gagtctgaga tggaagttgt caactgtcca gagaaaggtc tgcccctcta 13680 tctcatagtg ggggtcagtg caggaggact cctcttggtg ttctttgggg cgctgtttat 13740 tttctgtatc tgcaagagga aaaaacggaa caggaggaga aaaggtgaag agctggagat 13800 aaaagcttcc agaatgagca ctgtggaaag gggacccaag ccacactcaa cccaggcctc 13860 agcaccagca tcacagaatc cagtggcttc ccaagctcct cctccacctg gccatcatct 13920 ccagacacct ggccatcgtc ccctgcctcc aagccaccgt aaccgtgagc accagccgaa 13980 gaagagacct cctccatcag gcacacaggt tcaccagcag aaaggccctc ctttgcccag 14040 accccgagtt cagccaaagc ctccctgtgg gagtggagat gtttcgttgc caccccctaa 14100 tgagaacgca aacagctttt gctatgaaaa tgaagttgcc ctctaaaatg aatcgttttt 14160 aaaataacaa atcaattgtt ttataatatt cgtacgattc tttgattatg taataaaatg 14220 tgatcattag gaagattacg aaaaatataa aaaatatgag ttctgtgtgt ataacaaatg 14280 ctgtaaacgc cacaattgtg tttgttgcaa ataaacccat gattatttga ttaaaattgt 14340 tgttttcttt gttcatagac aatagtgtgt tttgcctaaa cgtgtactgc ataaactcca 14400 tgcgagtgta tagcgagcta gtggctaacg cttgccccac caaagtagat tcgtcaaaat 14460 cctcaatttc atcaccctcc tccaagttta acatttggcc gtcggaatta acttctaaag 14520 atgccacata atctaataaa tgaaatagag attcaaacgt ggcgtcatcg tccgtttcga 14580 ccatttccga aaagaactcg ggcataaact ctatgatttc tctggacgtg gtgttgtcga 14640 aactctcaaa gtacgcagtc aggaacgtgc gcgacatgtc gtcgggaaac tcgcgcggaa 14700 acatgttgtt gtaaccgaac gggtcccata gcgccaaaac caaatctgcc agcgtcaata 14760 gaatgagcac gatgccgaca atggagctgg cttggatagc gattcgagtt aacccggttt 14820 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg 14880 tttctta 14887 // ID MN658778; SV 1; linear; other DNA; STD; SYN; 14866 BP. XX AC MN658778; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct B2 recombinase and HBMSP::BoNTA::GFPnb::CD2 genes, DE complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-14866 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 4275debc1707b16c899356f14551b9b5. XX FH Key Location/Qualifiers FH FT source 1..14866 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7332,14127..14866) FT /note="pJFRC161" FT regulatory 6508..6602 FT /note="5xGal4_DBD" FT /regulatory_class="enhancer" FT regulatory 6636..6730 FT /note="5xGal4_DBD" FT /regulatory_class="enhancer" FT regulatory 6783..6910 FT /note="5xGal4_DBD" FT /regulatory_class="enhancer" FT regulatory 6911..6982 FT /note="5xGal4_DBD" FT /regulatory_class="enhancer" FT regulatory 7009..7236 FT /note="HS promoter" FT /regulatory_class="promoter" FT misc_feature 7261..7332 FT /note="IVS" FT misc_feature 7333..7365 FT /note="B3RT" FT 5'UTR 7366..7386 FT /note="Syn21" FT CDS 7387..8811 FT /codon_start=1 FT /transl_table=11 FT /product="B2 recombinase" FT /protein_id="QGY73544.1" FT /translation="MSEFSELVRILPLDQVAEIKRILSRGDPIPLQRLASLLTMVILTV FT NMSKKRKSSPIKLSTFTKYRRNVAKSLYYDMSSKTVFFEYHLKNTQDLQEGLEQAIAPY FT NFVVKVHKKPIDWQKQLSSVHERKAGHRSILSNNVGAEISKLAETKDSTWSFIERTMDL FT IEARTRQPTTRVAYRFLLQLTFMNCCRANDLKNADPSTFQIIADPHLGRILRAFVPETK FT TSIERFIYFFPCKGRCDPLLALDSYLLWVGPVPKTQTTDEETQYDYQLLQDTLLISYDR FT FIAKESKENIFKIPNGPKAHLGRHLMASYLGNNSLKSEATLYGNWSVERQEGVSKMADS FT RYMHTVKKSPPSYLFAFLSGYYKKSNQGEYVLAETLYNPLDYDKTLPITTNEKLICRRY FT GKNAKVIPKDALLYLYTYAQQKRKQLADPNEQNRLFSSESPAHPFLTPQSTGSSTPLTW FT TAPKTLSTGLMTPGEE" FT 3'UTR 8817..9059 FT /note="Hsp70B" FT 3'UTR 9138..9834 FT /note="SV40" FT misc_feature 9836..9868 FT /note="B3RT" FT 5'UTR 9887..9907 FT /note="Syn21" FT CDS 9908..14125 FT /codon_start=1 FT /transl_table=11 FT /product="HBMSP::BoNTA::GFPnb::CD2" FT /protein_id="QGY73545.1" FT /translation="MKFLVNVALVFMVVYISYIYAAMPFVNKQFNYKDPVNGVDIAYIK FT IPNAGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLNPPPEAKQVPVSYYDSTYLSTDN FT EKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGGSTIDTELKVIDTNCINVIQPD FT GSYRSEELNLVIIGPSADIIQFECKSFGHEVLNLTRNGYGSTQYIRFSPDFTFGFEESL FT EVDTNPLLGAGKFATDPAVTLAHELIHAGHRLYGIAINPNRVFKVNTNAYYEMSGLEVS FT FEELRTFGGHDAKFIDSLQENEFRLYYYNKFKDIASTLNKAKSIVGTTASLQYMKNVFK FT EKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVKFFKVLNRKTYLNFDKAVFKIN FT IVPKVNYTIYDGFNLRNTNLAANFNGQNTEINNMNFTKLKNFTGLFEFYKLLCVRGIIT FT SKTKSLDKGYNKAKSGSENLYFQGSGLVPRGSQALNDLCIKVNNWDLFFSPSEDNFTND FT LNKGEEITSDTNIEAAEENISLDLIQQYYLTFNFDNEPENISIENLSSDIIGQLELMPN FT IERFPNGKKYELDKYTMFHYLRAQEFEHGKSRIALTNSVNEALLNPSRVYTFFSSDYVK FT KVNKATEAAMFLGWVEQLVYDFTDETSEVSTTDKIADITIIIPYIGPALNIGNMLYKDD FT FVGALIFSGAVILLEFIPEIAIPVLGTFALVSYIANKVLTVQTIDNALSKRNEKWDEVY FT KYIVTNWLAKVNTQIDLIRKKMKEALENQAEATKAIINYQYNQYTEEEKNNINFNIDDL FT SSKLNESINKAMININKFLNQCSVSYLMNSMIPYGVKRLEDFDASLKDALLKYIYDNRG FT TLIGQVDRLKDKVNNTLSTDIPFQLSKYVDNQRLLSTFTEYIKNIRSGMQVQLVESGGA FT LVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKEREWVAGMSSAGDRSSYEDSVKGRFT FT ISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYWGQGTQVTVSSKSGGYPYDVPDYA FT GSENLYFQGGSLVPRGSGSDCRDSGTVWGALGHGINLNIPNFQMTDDIDEVRWERGSTL FT VAEFKRKMKPFLKSGAFEILANGDLKIKNLTRDDSGTYNVTVYSTNGTRILDKALDLRI FT LEMVSKPMIYWECSNATLTCEVLEGTDVELKLYQGKEHLRSLRQKTMSYQWTNLRAPFK FT CKAVNRVSQESEMEVVNCPEKGLPLYLIVGVSAGGLLLVFFGALFIFCICKRKKRNRRR FT KGEELEIKASRMSTVERGPKPHSTQASAPASQNPVASQAPPPPGHHLQTPGHRPLPPSH FT RNREHQPKKRPPPSGTQVHQQKGPPLPRPRVQPKPPCGSGDVSLPPPNENANSFCYENE FT VAL" FT misc_feature 9908..11323 FT /note="BoNT/A1 light chain" FT sig_peptide 9908..9973 FT /note="from honeybee melittin (MBP)" FT misc_feature 11333..11353 FT /note="TEV site" FT misc_feature 11360..11377 FT /note="thrombin site" FT misc_feature 11384..12655 FT /note="BoNT/A1 translocation domain" FT misc_feature 12665..13015 FT /note="Anti-GFP nanobody" FT misc_feature 13025..13051 FT /note="1xHA tag" FT misc_feature 13058..13078 FT /note="TEV site" FT misc_feature 13085..13102 FT /note="thrombin site" FT misc_feature 13109..14080 FT /note="CD2 from rat" FT misc_feature 13658..13726 FT /note="transmembrane domain" FT misc_feature 14084..14122 FT /note="ER export signal from Kir2.1" FT 3'UTR 14127..14792 FT /note="P10" XX SQ Sequence 14866 BP; 3920 A; 3795 C; 3594 G; 3557 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggtcgg agtactgtcc tccgagcgga gtactgtcct 6540 ccgagcggag tactgtcctc cgagcggagt actgtcctcc gagcggagta ctgtcctccg 6600 agcggagact ctagccctag ggcatgcctg caggtcggag tactgtcctc cgagcggagt 6660 actgtcctcc gagcggagta ctgtcctccg agcggagtac tgtcctccga gcggagtact 6720 gtcctccgag cggagactct agcgctagcg catgcctgca ggtcggagta ctgtcctccg 6780 agcggagtac tgtcctccga gcggagtact gtcctccgag cggagtactg tcctccgagc 6840 ggagtactgt cctccgagcg gagactctag cactagtgca tgcctgcagg tcggagtact 6900 gtcctccgag cggagtactg tcctccgagc ggagtactgt cctccgagcg gagtactgtc 6960 ctccgagcgg agtactgtcc tccgagcgga gactctagcg acgtcgagcg ccggagtata 7020 aatagaggcg cttcgtctac ggagcgacaa ttcaattcaa acaagcaaag tgaacacgtc 7080 gctaagcgaa agctaagcaa ataaacaagc gcagctgaac aagctaaaca atctgcagta 7140 aagtgcaagt taaagtgaat caattaaaag taaccagcaa ccaagtaaat caactgcaac 7200 tactgaaatc tgccaagaag taattattga atacaagaag agaactctga atagatctaa 7260 aaggtaggtt caaccactga tgcctaggca caccgaaacg actaacccta attcttatcc 7320 tttacttcag gcggttgctt aagaataagt aattcttaag caaccaactt aaaaaaaaaa 7380 atcaaaatgt cggaatttag tgagttggta cgtatcttgc ctttggatca ggttgcggag 7440 atcaaacgta ttctgagccg tggcgatccc atcccactcc agcgcctggc ttcgctgctg 7500 accatggtga ttttgacggt gaacatgtcc aaaaagcgca aatcgtcccc tataaaactg 7560 tcgaccttca ccaagtaccg acgcaacgtg gctaagtcgt tgtactacga tatgtcgtcc 7620 aagacagtgt tctttgagta ccacttgaag aacactcagg acctgcagga aggcctggag 7680 caggccatcg ctccgtacaa cttcgtagtg aaggtgcata agaaacccat agactggcag 7740 aaacaactta gtagcgtcca cgaacgcaag gcaggccacc gttcgatcct gtccaataat 7800 gtgggcgccg agatttccaa gctggcagag accaaagata gcacttggtc gttcatcgaa 7860 cgaaccatgg acctgatcga ggctcgcacc cgtcagccca ccacccgggt tgcatatcgg 7920 tttctgctgc agctgacgtt catgaactgt tgccgtgcga atgacttgaa gaacgccgat 7980 cccagcacgt tccagatcat tgctgatccc cacctgggtc gtatcctccg cgcctttgtg 8040 cccgagacaa agaccagtat cgagcggttt atctacttct tcccatgtaa aggccgctgc 8100 gatccactcc ttgccttgga cagttacctg ttgtgggtgg gcccggtgcc caagacgcag 8160 accacggatg aagagacgca atacgactat cagctgttgc aagatacact ccttatcagc 8220 tacgatcgct tcatagccaa agaaagtaag gaaaatatct tcaagattcc caacggaccg 8280 aaggctcacc tgggccgcca cctcatggcc tcgtaccttg gaaataattc gcttaagtcg 8340 gaggctacgc tgtacggtaa ctggtcggtg gaacggcaag agggagtttc gaaaatggcc 8400 gacagccgat acatgcacac cgttaagaaa agccctcctt cctacctctt cgcctttttg 8460 tcgggttatt acaagaagag taaccaaggc gaatacgtgc ttgcagaaac cctctacaac 8520 cccttggatt acgataaaac actgccaata actaccaatg agaagttgat ctgtcgccgg 8580 tatggtaaga acgcgaaggt gatccccaaa gatgccctgt tgtatctgta cacctatgct 8640 cagcagaagc gtaaacagct tgccgatccg aatgaacaga atcgcctgtt ttcgagcgaa 8700 tccccggcac accccttcct gactccccag tcgacgggca gctcgacgcc gctgacctgg 8760 accgcaccga agacgctttc caccggcctg atgacaccgg gcgaagagta agaattgact 8820 aaagccaaat agaaaattat tcagttcctg gcttaagttt ttaaaagtga tattatttat 8880 ttggttgtaa ccaaccaaaa gaatgtaaat aactaataca taattatgtt agttttaagt 8940 tagcaacaaa ttgattttag ctatattagc tacttggtta ataaatagaa tatatttatt 9000 taaagataat tgcgttttta ttgtcaggga gtgagtttgc ttaaaaactc gtttagatcc 9060 actagttcta gttgcggccg gctaagaatt cgatatcaag cttatcgata ccgtcgacct 9120 cgagggtacc tctagaggat ctttgtgaag gaaccttact tctgtggtgt gacataattg 9180 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa 9240 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat 9300 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg 9360 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag 9420 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct 9480 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca 9540 ctgctataca agaaaattat ggaaaaatat ttgatgtata gtgccttgac tagagatcat 9600 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc 9660 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta 9720 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 9780 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcggttg 9840 cttaagaata agtaattctt aagcaaccca aatccgccac gaatcaaact taaaaaaaaa 9900 aatcaaaatg aaattcctcg tgaacgtggc cctggtgttc atggtggtgt acatcagcta 9960 catctacgcc gccatgccct tcgtgaacaa gcagttcaac tacaaggatc ccgtgaacgg 10020 cgtggacatt gcctacatca agatccccaa cgccggccag atgcagcccg tgaaggcctt 10080 taagatccac aacaagatct gggtcatccc cgagcgcgat accttcacca accccgagga 10140 aggcgatctg aaccccccac ccgaagccaa gcaggtcccc gtgtcctact acgatagcac 10200 ctacctgagc accgataacg agaaggataa ctacctgaag ggcgtgacca agctgttcga 10260 gcgcatctac agcaccgatc tgggccgcat gctgctgacc tcgatcgtgc gcggcatccc 10320 gttctggggc ggcagcacca tcgataccga gctgaaggtc atcgatacca actgcatcaa 10380 cgtgatccag cccgatggca gctaccgcag cgaggaactg aacctggtca tcatcggccc 10440 cagcgccgat atcatccagt tcgagtgcaa gagcttcggc cacgaggtgc tgaacctgac 10500 ccgcaacggc tacggcagca cccagtacat ccgcttcagc cccgatttca ccttcggctt 10560 cgaggaatcc ctggaagtgg ataccaaccc cctgctggga gccggcaagt tcgccaccga 10620 tccagccgtg accctggccc acgagctgat tcatgccggc caccgcctgt acggcattgc 10680 catcaacccc aaccgcgtgt tcaaggtcaa caccaacgcc tactacgaga tgagcggcct 10740 ggaagtgtcc ttcgaggaac tgcgcacctt cggcggacac gatgccaagt tcatcgatag 10800 cctgcaggaa aacgagttcc gcctgtacta ctacaacaag ttcaaggata tcgccagcac 10860 cctgaacaag gcgaagagca tcgtgggcac caccgccagc ctgcagtaca tgaagaatgt 10920 gttcaaggaa aagtacctgc tgagcgagga taccagcggc aagttctccg tggataagct 10980 gaagttcgac aagctgtaca agatgctgac cgagatctac accgaggaca acttcgtgaa 11040 gttcttcaag gtgctgaatc gcaagaccta cctgaacttc gataaggccg tgttcaagat 11100 taacatcgtg cccaaggtca actacaccat ctacgatggc ttcaacctgc gcaacaccaa 11160 cctggccgcc aacttcaacg gccagaacac cgagatcaac aacatgaact tcaccaagct 11220 gaagaatttc accggcctgt tcgagttcta caagctgctg tgcgtgcgcg gaatcatcac 11280 cagcaagacc aagagcctgg ataagggcta caacaaggcc aagtccggca gcgagaacct 11340 gtattttcaa ggcagcggcc tggtgccacg cggctcccag gccctgaacg atctgtgcat 11400 taaggtcaac aactgggacc tgttcttcag ccccagcgag gataatttca ccaacgatct 11460 gaacaagggc gaggaaatca ccagcgatac caacatcgag gccgccgagg agaacatcag 11520 cctggacctg atccagcagt actacctgac cttcaacttc gataacgagc ccgagaacat 11580 ctccatcgag aacctgagca gcgatatcat cggccagctg gaactgatgc ccaacatcga 11640 gcgcttcccc aacggcaaga agtacgagct ggataagtac accatgttcc actacctgcg 11700 cgcccaggaa ttcgagcacg gcaagagccg cattgccctg accaacagcg tgaacgaggc 11760 cctgctgaac cccagccgcg tgtacacctt cttcagcagc gattacgtga agaaggtcaa 11820 caaggccacc gaggccgcca tgtttctggg ctgggtggaa cagctggtgt acgatttcac 11880 cgatgagaca agcgaggtgt ccaccaccga taagatcgcc gatatcacca tcatcatccc 11940 gtacatcggc ccagccctga acatcggcaa catgctgtac aaggatgatt tcgtgggcgc 12000 cctgatcttc agcggagccg tgatcctgct ggaattcatc cccgagatcg ccatccccgt 12060 gctgggcacc ttcgccctgg tgtcctacat tgccaacaag gtgctgaccg tgcagaccat 12120 cgataacgcc ctgagcaagc gcaacgagaa gtgggatgag gtgtacaagt acatcgtgac 12180 caactggctg gccaaggtca acacccagat cgacctgatc cgcaagaaga tgaaggaagc 12240 cctggaaaac caggccgagg ccaccaaggc catcatcaac taccagtaca atcagtacac 12300 cgaggaagag aagaacaaca tcaacttcaa catcgacgac ctgtccagca agctgaacga 12360 gagcattaac aaggccatga ttaacatcaa caagtttctg aaccagtgca gcgtgtccta 12420 cctgatgaac agcatgatcc cctacggcgt gaagcgcctg gaagatttcg atgccagcct 12480 gaaggatgcc ctgctgaagt acatctacga taaccgcggc accctgatcg gccaggtgga 12540 ccgcctgaag gataaggtca acaataccct gtccaccgat atcccgttcc agctgtcgaa 12600 gtacgtggac aaccagcgtc tgctgagcac cttcaccgag tacatcaaga acatccgcag 12660 cggcatgcag gtccagctgg tggaaagtgg cggagccctg gtgcagccag gtggaagtct 12720 gcgactgagc tgcgccgcca gcggcttccc cgtgaatcgc tacagcatgc gctggtatcg 12780 ccaggcccca ggcaaggaac gcgagtgggt ggccggaatg agcagtgccg gcgatcgcag 12840 cagctacgag gatagcgtca agggccgctt caccatcagc cgcgacgatg cccgcaacac 12900 cgtgtacctg cagatgaaca gcctgaagcc cgaggatacc gccgtgtact actgcaacgt 12960 gaacgtgggc ttcgagtact ggggccaggg cacccaggtc acagtgtcct ccaagagcgg 13020 cggctacccc tacgatgtgc ccgattacgc cggcagcgag aacctgtatt ttcaaggcgg 13080 cagcctggtg ccacgcggca gtggaagtga ctgcagagac agtgggaccg tctggggtgc 13140 cctgggtcat ggcatcaacc tgaacatccc taactttcaa atgactgatg atattgatga 13200 ggtgcgatgg gagaggggga gcaccctggt tgccgagttt aaaaggaaga tgaagccttt 13260 tttgaaatcg ggagcatttg agatcttagc aaatggagac ttgaagataa agaatctgac 13320 aagagatgac agtggcacct ataatgtaac ggtatacagc acaaatggga cacgtatcct 13380 ggacaaggca ctggacttga ggatcctaga gatggtctca aagccgatga tctactggga 13440 gtgcagcaac gcaaccctga cctgtgaggt cttggaagga acagatgttg aactaaagct 13500 gtaccaagga aaggagcatc tcaggagcct ccgtcagaag accatgagtt accagtggac 13560 caacctgaga gcaccgttta agtgcaaggc ggtaaacagg gtcagccagg agtctgagat 13620 ggaagttgtc aactgtccag agaaaggtct gcccctctat ctcatagtgg gggtcagtgc 13680 aggaggactc ctcttggtgt tctttggggc gctgtttatt ttctgtatct gcaagaggaa 13740 aaaacggaac aggaggagaa aaggtgaaga gctggagata aaagcttcca gaatgagcac 13800 tgtggaaagg ggacccaagc cacactcaac ccaggcctca gcaccagcat cacagaatcc 13860 agtggcttcc caagctcctc ctccacctgg ccatcatctc cagacacctg gccatcgtcc 13920 cctgcctcca agccaccgta accgtgagca ccagccgaag aagagacctc ctccatcagg 13980 cacacaggtt caccagcaga aaggccctcc tttgcccaga ccccgagttc agccaaagcc 14040 tccctgtggg agtggagatg tttcgttgcc accccctaat gagaacgcaa acagcttttg 14100 ctatgaaaat gaagttgccc tctaaaatga atcgttttta aaataacaaa tcaattgttt 14160 tataatattc gtacgattct ttgattatgt aataaaatgt gatcattagg aagattacga 14220 aaaatataaa aaatatgagt tctgtgtgta taacaaatgc tgtaaacgcc acaattgtgt 14280 ttgttgcaaa taaacccatg attatttgat taaaattgtt gttttctttg ttcatagaca 14340 atagtgtgtt ttgcctaaac gtgtactgca taaactccat gcgagtgtat agcgagctag 14400 tggctaacgc ttgccccacc aaagtagatt cgtcaaaatc ctcaatttca tcaccctcct 14460 ccaagtttaa catttggccg tcggaattaa cttctaaaga tgccacataa tctaataaat 14520 gaaatagaga ttcaaacgtg gcgtcatcgt ccgtttcgac catttccgaa aagaactcgg 14580 gcataaactc tatgatttct ctggacgtgg tgttgtcgaa actctcaaag tacgcagtca 14640 ggaacgtgcg cgacatgtcg tcgggaaact cgcgcggaaa catgttgttg taaccgaacg 14700 ggtcccatag cgccaaaacc aaatctgcca gcgtcaatag aatgagcacg atgccgacaa 14760 tggagctggc ttggatagcg attcgagtta acccggtttg aagacgaaag ggcctcgtga 14820 tacgcctatt tttataggtt aatgtcatga taataatggt ttctta 14866 // ID MN658779; SV 1; linear; other DNA; STD; SYN; 9994 BP. XX AC MN658779; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct Syb::GFP gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-9994 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 61f75bd208ff7211eeb976a62873ba8c. XX FH Key Location/Qualifiers FH FT source 1..9994 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,9199..9994) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT 5'UTR 7212..7232 FT /note="Syn21" FT CDS 7233..8525 FT /codon_start=1 FT /transl_table=11 FT /product="Syb::GFP" FT /protein_id="QGY73546.1" FT /translation="MADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQT FT QAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLK FT MMIIMGVIGLVVVGIIANKLGLIGGEQPPQYQYPPQYMQPPPPPPQQPAGGQSSLVDGA FT GAGDGAGGSAGAGDHGGVGGGGSGGGGSSMSKGEELFTGVVPILVELDGDVNGHKFSVS FT GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMP FT EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSH FT NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSAL FT SKDPNEKRDHMVLLEFVTAAGITHGMDELYK" FT misc_feature 7233..7775 FT /note="Drosophila synaptobrevin" FT misc_feature 7548..7613 FT /note="trans-membrane segment" FT misc_feature 7808..8525 FT /note="Drosophila codon optimised EGFP" FT 3'UTR 8526..9197 FT /note="P10" FT 3'UTR 9201..9897 FT /note="SV40" XX SQ Sequence 9994 BP; 2665 A; 2352 C; 2387 G; 2590 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg caacttaaaa aaaaaaatca aaatggcgga cgctgcacca gctggcgatg 7260 caccacccaa tgccggagcc ccggccggag agggcggcga tggcgagatt gtgggcggac 7320 cacacaatcc gcagcagatc gcggcacaga agcgtctgca gcagacgcag gcgcaggtcg 7380 atgaggtcgt ggacatcatg cgcacgaacg tggaaaaggt gctggagcgc gacagcaagc 7440 tgtcggagct ggacgaccgt gccgatgcct tgcagcaggg tgcctcgcag tttgagcagc 7500 aggcgggcaa gctcaagagg aaattctggc tccagaactt gaagatgatg atcatcatgg 7560 gcgtgattgg cctggttgtc gtgggcatta ttgcaaataa acttggactc ataggcgggg 7620 agcagccgcc acagtaccag tatcccccac agtacatgca gccaccgccg ccgccaccgc 7680 agcagccagc cggaggacag tcatcgctgg tggatggggc aggagcagga gacggagcag 7740 gaggatcggc aggagctggc gatcacggcg gcgtgggtgg cggcggaagt ggaggtggag 7800 gctcgtccat gtccaaaggt gaagaactgt ttaccggagt agtcccgata ttggttgaac 7860 tcgacggcga tgtcaacggt cataaattca gtgtgtccgg cgagggtgag ggcgacgcca 7920 catacggtaa gctgacgttg aagttcatat gcaccacggg caagctgccc gtgccatggc 7980 cgacgttggt cacgacgctg acgtatggtg tccagtgttt cagccgttac cccgatcata 8040 tgaagcagca tgactttttc aagtcggcga tgccggaggg atacgttcaa gagaggacca 8100 ttttcttcaa ggatgacggc aactataaga cgcgagcgga ggtgaaattt gaaggcgaca 8160 cactcgttaa ccgtattgag ttgaagggca ttgattttaa ggaggatggc aacattctgg 8220 gccataagtt ggaatacaac tacaactcgc ataatgtgta tataatggca gataagcaga 8280 aaaacggaat aaaggttaac ttcaagattc gccacaacat agaggacggt tccgtgcaac 8340 ttgcagatca ttaccaacag aacacaccca ttggagatgg cccagttctc ttgccagaca 8400 atcactacct ttccacacag tccgcgttga gcaaggaccc caatgaaaag cgggaccaca 8460 tggtgttgct ggagtttgtg accgcagctg gtattacaca cggcatggat gagctctaca 8520 agtaatctag aatgaatcgt ttttaaaata acaaatcaat tgttttataa tattcgtacg 8580 attctttgat tatgtaataa aatgtgatca ttaggaagat tacgaaaaat ataaaaaata 8640 tgagttctgt gtgtataaca aatgctgtaa acgccacaat tgtgtttgtt gcaaataaac 8700 ccatgattat ttgattaaaa ttgttgtttt ctttgttcat agacaatagt gtgttttgcc 8760 taaacgtgta ctgcataaac tccatgcgag tgtatagcga gctagtggct aacgcttgcc 8820 ccaccaaagt agattcgtca aaatcctcaa tttcatcacc ctcctccaag tttaacattt 8880 ggccgtcgga attaacttct aaagatgcca cataatctaa taaatgaaat agagattcaa 8940 acgtggcgtc atcgtccgtt tcgaccattt ccgaaaagaa ctcgggcata aactctatga 9000 tttctctgga cgtggtgttg tcgaaactct caaagtacgc agtcaggaac gtgcgcgaca 9060 tgtcgtcggg aaactcgcgc ggaaacatgt tgttgtaacc gaacgggtcc catagcgcca 9120 aaaccaaatc tgccagcgtc aatagaatga gcacgatgcc gacaatggag ctggcttgga 9180 tagcgattcg agttaactag gatctttgtg aaggaacctt acttctgtgg tgtgacataa 9240 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta 9300 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact 9360 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa 9420 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag 9480 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat 9540 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct 9600 gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat 9660 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct 9720 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc 9780 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 9840 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcga 9900 tctggccggc cgtttaaacg aattcttgaa gacgaaaggg cctcgtgata cgcctatttt 9960 tataggttaa tgtcatgata ataatggttt ctta 9994 // ID MN658780; SV 1; linear; other DNA; STD; SYN; 10183 BP. XX AC MN658780; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct QF2::V5::SNAP25::syntaxin gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-10183 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 0bee8ed7c3dae7587af8116ea08d017d. XX FH Key Location/Qualifiers FH FT source 1..10183 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,9388..10183) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT 5'UTR 7212..7232 FT /note="Syn21" FT CDS 7233..9386 FT /codon_start=1 FT /transl_table=11 FT /product="QF2::V5::SNAP25::syntaxin" FT /protein_id="QGY73547.1" FT /translation="MPPKRKTLNAAAEANAHADGHADGNADGHVANTAASSNNARFADL FT TNIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCRAAREKCDGIQPACFPCVSQGRSCT FT YQASPKKRGVQTGYIRTLELALAWMFENVARSEDALHNLLVRDAGQGSALLVGKDSPAA FT ERLHARWATSRVNKSITRLLATASIMPHVMEQPLSTSINPVNDRFNGIPNPTPYNSDAA FT LDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPPYVDSTTNHPPY FT HSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPGCNFSDINTYEQ FT GKPIPNPLLGLDSTARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKA FT DSNKTRIDEANQRATKMLGSGDDDDKQMTKDRLAALHAAQSDDEEETEVAVNVDGHDSY FT MDDFFAQVEEIRGMIDKVQDNVEEVKKKHSAILSAPQTDEKTKQELEDLMADIKKNANR FT VRGKLKGIEQNIEQEEQQNKSSADLRIRKTQHSTLSRKFVEVMTEYNRTQTDYRERCKG FT RIQRQLEITGRPTNDDELEKMLEEGNSSVFTQGIIMETQQAKQTLADIEARHQDIMKLE FT TSIKELHDMFMDMAMLVESQGEMIDRIEYHVEHAMDYVQTATQDTKKALKYQSKARRKK FT IMILICLTVLGILAASYVSSYFM" FT misc_feature 7233..8252 FT /note="QF2" FT misc_feature 8253..8294 FT /note="1xV5 tag" FT misc_feature 8295..8492 FT /note="encodes amino acids 141-206 of human SNAP25" FT misc_feature 8493..8507 FT /note="1xFLAG tag" FT misc_feature 8511..9386 FT /note="Drosophila syntaxin" FT 3'UTR 9387..10086 FT /note="SV40" XX SQ Sequence 10183 BP; 2695 A; 2495 C; 2438 G; 2555 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg caacttaaaa aaaaaaatca aaatgccgcc taaacgcaag acactcaatg 7260 ccgctgccga agccaatgcc cacgctgatg gccatgctga tggcaatgct gatggtcatg 7320 tcgctaacac tgcagcaagc agcaacaacg cccgttttgc ggacttgacc aacattgaca 7380 cacccggcct cggccctacc acgacgacgt tacttgtcga gcccgctcgt tcgaaacgcc 7440 agagagtctc gagggcctgt gatcagtgtc gagctgcacg tgaaaagtgt gatggaatcc 7500 agccggcttg cttcccctgt gtgtcgcagg gccggtcgtg tacctaccag gccagtccca 7560 agaagcgagg agtccagacg ggctacatcc gcactctcga actggctctg gcttggatgt 7620 tcgagaacgt tgcccgcagc gaggacgccc tccacaatct tttggtccgt gatgctggcc 7680 agggcagcgc tctcctggtc ggcaaagact cgcctgctgc agaacgcctg catgcaagat 7740 gggcgacgag tcgagtcaac aaaagcatca cccgtcttct cgccactgca agtatcatgc 7800 cccatgtcat ggaacagccc ctgtcaacgt caataaatcc ggtcaatgat cggtttaatg 7860 gaataccgaa tcctaccccc tacaatagcg atgcggccct agacgcaatt actcaaacca 7920 atgactacgg atcagtcaat acccatggca ttctcagtac atatccacca cctgccaccc 7980 acttgaacga ggcctcggtg gcccttgcgc ctggaggtgc tcctcccaga ccacccccgc 8040 catacgttga tagtacaacg aaccatcctc cttaccatag taacctcgtc cccatggcca 8100 atttcggata ttcgactgta gactatgatg cgatggtgga tgatctggca tcgatcgagt 8160 acacggatgc ggttgacgtt gatccgcagt tcatgacgaa cctcgggttt gtcccagggt 8220 gtaattttag cgatatcaac acgtatgagc aaggtaagcc tatccctaac cctctccttg 8280 gtctcgattc tacggcccga gaaaatgaaa tggatgaaaa cctagagcag gtgagcggca 8340 tcatcgggaa cctccgtcac atggccctgg atatgggcaa tgagatcgat acacagaatc 8400 gccagatcga caggatcatg gagaaggctg attccaacaa aaccagaatt gatgaggcca 8460 accaacgtgc aacaaagatg ctgggaagtg gtgatgacga tgacaagcaa atgactaaag 8520 acagattagc cgctctccat gccgcccaat ccgatgacga ggaggagacg gaggtggccg 8580 tcaatgtgga tggccatgat tcctacatgg acgacttctt cgcccaggtg gaggagatcc 8640 gcgggatgat cgacaaggtg caggataacg tcgaggaggt aaagaagaag cactcggcca 8700 tcctgtccgc cccacaaacg gacgagaaga ccaagcaaga gctggaggat ctgatggccg 8760 acatcaagaa gaatgccaat cgcgttcgcg gcaagctcaa gggcatcgag cagaacatcg 8820 agcaggaaga gcagcagaat aagtcgtcgg cggatctgag aattcggaag acgcagcact 8880 cgacgttgtc acggaaattc gtcgaagtga tgaccgagta caatcgcacg cagaccgact 8940 accgagagcg ctgcaaggga aggatacagc gtcaactgga gattaccgga cgaccgacca 9000 acgacgatga gctggagaag atgctggagg agggcaactc gtctgtgttc acgcagggca 9060 tcatcatgga gacgcagcag gccaaacaga cgctggcgga cattgaggcc cgccaccagg 9120 acatcatgaa gctggagaca tcgatcaagg agctgcacga catgttcatg gacatggcca 9180 tgctggtgga gtcgcagggc gagatgatcg atcgcattga gtaccatgtg gagcacgcta 9240 tggactatgt gcagacggca actcaggaca ccaagaaggc gctcaagtac cagagtaaag 9300 cccgacgaaa gaagatcatg atactgatct gcctcactgt gctgggcatc ttagcggcct 9360 catatgttag cagttatttc atgtaacagg atctttgtga aggaacctta cttctgtggt 9420 gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa tataaaattt 9480 ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta gattccaacc 9540 tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa cctgttttgc 9600 tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca ttctactcct 9660 ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt gctaagtttt 9720 ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta caccacaaag 9780 gaaaaagctg cactgctata caagaaaatt atggaaaaat atttgatgta tagtgccttg 9840 actagagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct 9900 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt 9960 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 10020 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 10080 ctggatcgat ctggccggcc gtttaaacga attcttgaag acgaaagggc ctcgtgatac 10140 gcctattttt ataggttaat gtcatgataa taatggtttc tta 10183 // ID MN658781; SV 1; linear; other DNA; STD; SYN; 10186 BP. XX AC MN658781; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct QF2::SNAP25::syntaxin gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-10186 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 86a6066f8c541b2f9463f09ee014ca46. XX FH Key Location/Qualifiers FH FT source 1..10186 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,9391..10186) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT 5'UTR 7212..7232 FT /note="Syn21" FT CDS 7233..9389 FT /codon_start=1 FT /transl_table=11 FT /product="QF2::SNAP25::syntaxin" FT /protein_id="QGY73548.1" FT /translation="MPPKRKTLNAAAEANAHADGHADGNADGHVANTAASSNNARFADL FT TNIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCRAAREKCDGIQPACFPCVSQGRSCT FT YQASPKKRGVQTGYIRTLELALAWMFENVARSEDALHNLLVRDAGQGSALLVGKDSPAA FT ERLHARWATSRVNKSITRLLATASIMPHVMEQPLSTSINPVNDRFNGIPNPTPYNSDAA FT LDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPPYVDSTTNHPPY FT HSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPGCNFSDINTYEQ FT GGSGGARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKADSNKTRIDE FT ANQRATKMLGSGSVSGLQLPPLERLTLQMTKDRLAALHAAQSDDEEETEVAVNVDGHDS FT YMDDFFAQVEEIRGMIDKVQDNVEEVKKKHSAILSAPQTDEKTKQELEDLMADIKKNAN FT RVRGKLKGIEQNIEQEEQQNKSSADLRIRKTQHSTLSRKFVEVMTEYNRTQTDYRERCK FT GRIQRQLEITGRPTNDDELEKMLEEGNSSVFTQGIIMETQQAKQTLADIEARHQDIMKL FT ETSIKELHDMFMDMAMLVESQGEMIDRIEYHVEHAMDYVQTATQDTKKALKYQSKARRK FT KIMILICLTVLGILAASYVSSYFM" FT misc_feature 7233..8252 FT /note="QF2" FT misc_feature 8268..8465 FT /note="encodes amino acids 141-206 of human SNAP25" FT misc_feature 8478..8510 FT /note="HIV nuclear export signal (NES)" FT misc_feature 8514..9389 FT /note="Drosophila syntaxin" FT 3'UTR 9390..10089 FT /note="SV40" XX SQ Sequence 10186 BP; 2692 A; 2496 C; 2448 G; 2550 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg caacttaaaa aaaaaaatca aaatgccgcc taaacgcaag acactcaatg 7260 ccgctgccga agccaatgcc cacgctgatg gccatgctga tggcaatgct gatggtcatg 7320 tcgctaacac tgcagcaagc agcaacaacg cccgttttgc ggacttgacc aacattgaca 7380 cacccggcct cggccctacc acgacgacgt tacttgtcga gcccgctcgt tcgaaacgcc 7440 agagagtctc gagggcctgt gatcagtgtc gagctgcacg tgaaaagtgt gatggaatcc 7500 agccggcttg cttcccctgt gtgtcgcagg gccggtcgtg tacctaccag gccagtccca 7560 agaagcgagg agtccagacg ggctacatcc gcactctcga actggctctg gcttggatgt 7620 tcgagaacgt tgcccgcagc gaggacgccc tccacaatct tttggtccgt gatgctggcc 7680 agggcagcgc tctcctggtc ggcaaagact cgcctgctgc agaacgcctg catgcaagat 7740 gggcgacgag tcgagtcaac aaaagcatca cccgtcttct cgccactgca agtatcatgc 7800 cccatgtcat ggaacagccc ctgtcaacgt caataaatcc ggtcaatgat cggtttaatg 7860 gaataccgaa tcctaccccc tacaatagcg atgcggccct agacgcaatt actcaaacca 7920 atgactacgg atcagtcaat acccatggca ttctcagtac atatccacca cctgccaccc 7980 acttgaacga ggcctcggtg gcccttgcgc ctggaggtgc tcctcccaga ccacccccgc 8040 catacgttga tagtacaacg aaccatcctc cttaccatag taacctcgtc cccatggcca 8100 atttcggata ttcgactgta gactatgatg cgatggtgga tgatctggca tcgatcgagt 8160 acacggatgc ggttgacgtt gatccgcagt tcatgacgaa cctcgggttt gtcccagggt 8220 gtaattttag cgatatcaac acgtatgagc aaggtggcag tggcggagcc cgagaaaatg 8280 aaatggatga aaacctagag caggtgagcg gcatcatcgg gaacctccgt cacatggccc 8340 tggatatggg caatgagatc gatacacaga atcgccagat cgacaggatc atggagaagg 8400 ctgattccaa caaaaccaga attgatgagg ccaaccaacg tgcaacaaag atgctgggaa 8460 gtggtagcgt aagcggtctg cagctgcctc cactggaacg cctgaccctg caaatgacta 8520 aagacagatt agccgctctc catgccgccc aatccgatga cgaggaggag acggaggtgg 8580 ccgtcaatgt ggatggccat gattcctaca tggacgactt cttcgcccag gtggaggaga 8640 tccgcgggat gatcgacaag gtgcaggata acgtcgagga ggtaaagaag aagcactcgg 8700 ccatcctgtc cgccccacaa acggacgaga agaccaagca agagctggag gatctgatgg 8760 ccgacatcaa gaagaatgcc aatcgcgttc gcggcaagct caagggcatc gagcagaaca 8820 tcgagcagga agagcagcag aataagtcgt cggcggatct gagaattcgg aagacgcagc 8880 actcgacgtt gtcacggaaa ttcgtcgaag tgatgaccga gtacaatcgc acgcagaccg 8940 actaccgaga gcgctgcaag ggaaggatac agcgtcaact ggagattacc ggacgaccga 9000 ccaacgacga tgagctggag aagatgctgg aggagggcaa ctcgtctgtg ttcacgcagg 9060 gcatcatcat ggagacgcag caggccaaac agacgctggc ggacattgag gcccgccacc 9120 aggacatcat gaagctggag acatcgatca aggagctgca cgacatgttc atggacatgg 9180 ccatgctggt ggagtcgcag ggcgagatga tcgatcgcat tgagtaccat gtggagcacg 9240 ctatggacta tgtgcagacg gcaactcagg acaccaagaa ggcgctcaag taccagagta 9300 aagcccgacg aaagaagatc atgatactga tctgcctcac tgtgctgggc atcttagcgg 9360 cctcatatgt tagcagttat ttcatgtaac aggatctttg tgaaggaacc ttacttctgt 9420 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa 9480 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca 9540 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt 9600 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact 9660 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt 9720 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca 9780 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatatttgat gtatagtgcc 9840 ttgactagag atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa 9900 cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt 9960 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 10020 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 10080 tgtctggatc gatctggccg gccgtttaaa cgaattcttg aagacgaaag ggcctcgtga 10140 tacgcctatt tttataggtt aatgtcatga taataatggt ttctta 10186 // ID MN658782; SV 1; linear; other DNA; STD; SYN; 10213 BP. XX AC MN658782; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct QF2::V5::SNAP25::HIVnes::Sytaxin gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-10213 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 7627eabbfc2657370c537e68388798a0. XX FH Key Location/Qualifiers FH FT source 1..10213 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,9418..10213) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT 5'UTR 7212..7232 FT /note="Syn21" FT CDS 7233..9416 FT /codon_start=1 FT /transl_table=11 FT /product="QF2::V5::SNAP25::HIVnes::Sytaxin" FT /protein_id="QGY73549.1" FT /translation="MPPKRKTLNAAAEANAHADGHADGNADGHVANTAASSNNARFADL FT TNIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCRAAREKCDGIQPACFPCVSQGRSCT FT YQASPKKRGVQTGYIRTLELALAWMFENVARSEDALHNLLVRDAGQGSALLVGKDSPAA FT ERLHARWATSRVNKSITRLLATASIMPHVMEQPLSTSINPVNDRFNGIPNPTPYNSDAA FT LDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPPYVDSTTNHPPY FT HSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPGCNFSDINTYEQ FT GKPIPNPLLGLDSTARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKA FT DSNKTRIDEANQRATKMLGSGSVSGLQLPPLERLTLQMTKDRLAALHAAQSDDEEETEV FT AVNVDGHDSYMDDFFAQVEEIRGMIDKVQDNVEEVKKKHSAILSAPQTDEKTKQELEDL FT MADIKKNANRVRGKLKGIEQNIEQEEQQNKSSADLRIRKTQHSTLSRKFVEVMTEYNRT FT QTDYRERCKGRIQRQLEITGRPTNDDELEKMLEEGNSSVFTQGIIMETQQAKQTLADIE FT ARHQDIMKLETSIKELHDMFMDMAMLVESQGEMIDRIEYHVEHAMDYVQTATQDTKKAL FT KYQSKARRKKIMILICLTVLGILAASYVSSYFM" FT misc_feature 7233..8252 FT /note="QF2" FT misc_feature 8253..8294 FT /note="1xV5 tag" FT misc_feature 8295..8492 FT /note="encodes amino acids 141-206 of human SNAP25" FT misc_feature 8505..8537 FT /note="HIV nuclear export signal (NES)" FT misc_feature 8541..9416 FT /note="Drosophila syntaxin" FT 3'UTR 9417..10116 FT /note="SV40" XX SQ Sequence 10213 BP; 2697 A; 2509 C; 2446 G; 2561 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg caacttaaaa aaaaaaatca aaatgccgcc taaacgcaag acactcaatg 7260 ccgctgccga agccaatgcc cacgctgatg gccatgctga tggcaatgct gatggtcatg 7320 tcgctaacac tgcagcaagc agcaacaacg cccgttttgc ggacttgacc aacattgaca 7380 cacccggcct cggccctacc acgacgacgt tacttgtcga gcccgctcgt tcgaaacgcc 7440 agagagtctc gagggcctgt gatcagtgtc gagctgcacg tgaaaagtgt gatggaatcc 7500 agccggcttg cttcccctgt gtgtcgcagg gccggtcgtg tacctaccag gccagtccca 7560 agaagcgagg agtccagacg ggctacatcc gcactctcga actggctctg gcttggatgt 7620 tcgagaacgt tgcccgcagc gaggacgccc tccacaatct tttggtccgt gatgctggcc 7680 agggcagcgc tctcctggtc ggcaaagact cgcctgctgc agaacgcctg catgcaagat 7740 gggcgacgag tcgagtcaac aaaagcatca cccgtcttct cgccactgca agtatcatgc 7800 cccatgtcat ggaacagccc ctgtcaacgt caataaatcc ggtcaatgat cggtttaatg 7860 gaataccgaa tcctaccccc tacaatagcg atgcggccct agacgcaatt actcaaacca 7920 atgactacgg atcagtcaat acccatggca ttctcagtac atatccacca cctgccaccc 7980 acttgaacga ggcctcggtg gcccttgcgc ctggaggtgc tcctcccaga ccacccccgc 8040 catacgttga tagtacaacg aaccatcctc cttaccatag taacctcgtc cccatggcca 8100 atttcggata ttcgactgta gactatgatg cgatggtgga tgatctggca tcgatcgagt 8160 acacggatgc ggttgacgtt gatccgcagt tcatgacgaa cctcgggttt gtcccagggt 8220 gtaattttag cgatatcaac acgtatgagc aaggtaagcc tatccctaac cctctccttg 8280 gtctcgattc tacggcccga gaaaatgaaa tggatgaaaa cctagagcag gtgagcggca 8340 tcatcgggaa cctccgtcac atggccctgg atatgggcaa tgagatcgat acacagaatc 8400 gccagatcga caggatcatg gagaaggctg attccaacaa aaccagaatt gatgaggcca 8460 accaacgtgc aacaaagatg ctgggaagtg gtagcgtaag cggtctgcag ctgcctccac 8520 tggaacgcct gaccctgcaa atgactaaag acagattagc cgctctccat gccgcccaat 8580 ccgatgacga ggaggagacg gaggtggccg tcaatgtgga tggccatgat tcctacatgg 8640 acgacttctt cgcccaggtg gaggagatcc gcgggatgat cgacaaggtg caggataacg 8700 tcgaggaggt aaagaagaag cactcggcca tcctgtccgc cccacaaacg gacgagaaga 8760 ccaagcaaga gctggaggat ctgatggccg acatcaagaa gaatgccaat cgcgttcgcg 8820 gcaagctcaa gggcatcgag cagaacatcg agcaggaaga gcagcagaat aagtcgtcgg 8880 cggatctgag aattcggaag acgcagcact cgacgttgtc acggaaattc gtcgaagtga 8940 tgaccgagta caatcgcacg cagaccgact accgagagcg ctgcaaggga aggatacagc 9000 gtcaactgga gattaccgga cgaccgacca acgacgatga gctggagaag atgctggagg 9060 agggcaactc gtctgtgttc acgcagggca tcatcatgga gacgcagcag gccaaacaga 9120 cgctggcgga cattgaggcc cgccaccagg acatcatgaa gctggagaca tcgatcaagg 9180 agctgcacga catgttcatg gacatggcca tgctggtgga gtcgcagggc gagatgatcg 9240 atcgcattga gtaccatgtg gagcacgcta tggactatgt gcagacggca actcaggaca 9300 ccaagaaggc gctcaagtac cagagtaaag cccgacgaaa gaagatcatg atactgatct 9360 gcctcactgt gctgggcatc ttagcggcct catatgttag cagttatttc atgtaacagg 9420 atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag 9480 atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt 9540 ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg 9600 aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag tgatgatgag 9660 gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt agaagacccc 9720 aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag taatagaact 9780 cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata caagaaaatt 9840 atggaaaaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt 9900 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa 9960 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag 10020 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 10080 gtccaaactc atcaatgtat cttatcatgt ctggatcgat ctggccggcc gtttaaacga 10140 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa 10200 taatggtttc tta 10213 // ID MN658783; SV 1; linear; other DNA; STD; SYN; 10216 BP. XX AC MN658783; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct HIVnes::QF2::SNAP25::syntaxin gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-10216 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; acc446b1619b9be806acaab7912cbafb. XX FH Key Location/Qualifiers FH FT source 1..10216 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,9421..10216) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT 5'UTR 7212..7232 FT /note="Syn21" FT CDS 7233..9419 FT /codon_start=1 FT /transl_table=11 FT /product="HIVnes::QF2::SNAP25::syntaxin" FT /protein_id="QGY73550.1" FT /translation="MLQLPPLERLTLPPKRKTLNAAAEANAHADGHADGNADGHVANTA FT ASSNNARFADLTNIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCRAAREKCDGIQPAC FT FPCVSQGRSCTYQASPKKRGVQTGYIRTLELALAWMFENVARSEDALHNLLVRDAGQGS FT ALLVGKDSPAAERLHARWATSRVNKSITRLLATASIMPHVMEQPLSTSINPVNDRFNGI FT PNPTPYNSDAALDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPP FT YVDSTTNHPPYHSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPG FT CNFSDINTYEQGKPIPNPLLGLDSTARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQ FT NRQIDRIMEKADSNKTRIDEANQRATKMLGSGGGSGGQMTKDRLAALHAAQSDDEEETE FT VAVNVDGHDSYMDDFFAQVEEIRGMIDKVQDNVEEVKKKHSAILSAPQTDEKTKQELED FT LMADIKKNANRVRGKLKGIEQNIEQEEQQNKSSADLRIRKTQHSTLSRKFVEVMTEYNR FT TQTDYRERCKGRIQRQLEITGRPTNDDELEKMLEEGNSSVFTQGIIMETQQAKQTLADI FT EARHQDIMKLETSIKELHDMFMDMAMLVESQGEMIDRIEYHVEHAMDYVQTATQDTKKA FT LKYQSKARRKKIMILICLTVLGILAASYVSSYFM" FT misc_feature join(7233..7235,7269..8285) FT /note="QF2" FT misc_feature 7236..7268 FT /note="HIV nuclear export signal (NES)" FT misc_feature 8286..8327 FT /note="1xV5 tag" FT misc_feature 8328..8525 FT /note="encodes amino acids 141-206 of human SNAP25" FT misc_feature 8544..9419 FT /note="Drosophila syntaxin" FT 3'UTR 9420..10119 FT /note="SV40" XX SQ Sequence 10216 BP; 2696 A; 2509 C; 2450 G; 2561 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg caacttaaaa aaaaaaatca aaatgctgca gctgcctcca ctggaacgcc 7260 tgaccctgcc gcctaaacgc aagacactca atgccgctgc cgaagccaat gcccacgctg 7320 atggccatgc tgatggcaat gctgatggtc atgtcgctaa cactgcagca agcagcaaca 7380 acgcccgttt tgcggacttg accaacattg acacacccgg cctcggccct accacgacga 7440 cgttacttgt cgagcccgct cgttcgaaac gccagagagt ctcgagggcc tgtgatcagt 7500 gtcgagctgc acgtgaaaag tgtgatggaa tccagccggc ttgcttcccc tgtgtgtcgc 7560 agggccggtc gtgtacctac caggccagtc ccaagaagcg aggagtccag acgggctaca 7620 tccgcactct cgaactggct ctggcttgga tgttcgagaa cgttgcccgc agcgaggacg 7680 ccctccacaa tcttttggtc cgtgatgctg gccagggcag cgctctcctg gtcggcaaag 7740 actcgcctgc tgcagaacgc ctgcatgcaa gatgggcgac gagtcgagtc aacaaaagca 7800 tcacccgtct tctcgccact gcaagtatca tgccccatgt catggaacag cccctgtcaa 7860 cgtcaataaa tccggtcaat gatcggttta atggaatacc gaatcctacc ccctacaata 7920 gcgatgcggc cctagacgca attactcaaa ccaatgacta cggatcagtc aatacccatg 7980 gcattctcag tacatatcca ccacctgcca cccacttgaa cgaggcctcg gtggcccttg 8040 cgcctggagg tgctcctccc agaccacccc cgccatacgt tgatagtaca acgaaccatc 8100 ctccttacca tagtaacctc gtccccatgg ccaatttcgg atattcgact gtagactatg 8160 atgcgatggt ggatgatctg gcatcgatcg agtacacgga tgcggttgac gttgatccgc 8220 agttcatgac gaacctcggg tttgtcccag ggtgtaattt tagcgatatc aacacgtatg 8280 agcaaggtaa gcctatccct aaccctctcc ttggtctcga ttctacggcc cgagaaaatg 8340 aaatggatga aaacctagag caggtgagcg gcatcatcgg gaacctccgt cacatggccc 8400 tggatatggg caatgagatc gatacacaga atcgccagat cgacaggatc atggagaagg 8460 ctgattccaa caaaaccaga attgatgagg ccaaccaacg tgcaacaaag atgctgggaa 8520 gtggtggtgg cagtggcgga caaatgacta aagacagatt agccgctctc catgccgccc 8580 aatccgatga cgaggaggag acggaggtgg ccgtcaatgt ggatggccat gattcctaca 8640 tggacgactt cttcgcccag gtggaggaga tccgcgggat gatcgacaag gtgcaggata 8700 acgtcgagga ggtaaagaag aagcactcgg ccatcctgtc cgccccacaa acggacgaga 8760 agaccaagca agagctggag gatctgatgg ccgacatcaa gaagaatgcc aatcgcgttc 8820 gcggcaagct caagggcatc gagcagaaca tcgagcagga agagcagcag aataagtcgt 8880 cggcggatct gagaattcgg aagacgcagc actcgacgtt gtcacggaaa ttcgtcgaag 8940 tgatgaccga gtacaatcgc acgcagaccg actaccgaga gcgctgcaag ggaaggatac 9000 agcgtcaact ggagattacc ggacgaccga ccaacgacga tgagctggag aagatgctgg 9060 aggagggcaa ctcgtctgtg ttcacgcagg gcatcatcat ggagacgcag caggccaaac 9120 agacgctggc ggacattgag gcccgccacc aggacatcat gaagctggag acatcgatca 9180 aggagctgca cgacatgttc atggacatgg ccatgctggt ggagtcgcag ggcgagatga 9240 tcgatcgcat tgagtaccat gtggagcacg ctatggacta tgtgcagacg gcaactcagg 9300 acaccaagaa ggcgctcaag taccagagta aagcccgacg aaagaagatc atgatactga 9360 tctgcctcac tgtgctgggc atcttagcgg cctcatatgt tagcagttat ttcatgtaac 9420 aggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca 9480 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg 9540 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg 9600 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat 9660 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac 9720 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga 9780 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa 9840 attatggaaa aatatttgat gtatagtgcc ttgactagag atcataatca gccataccac 9900 atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca 9960 taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata 10020 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg 10080 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc gatctggccg gccgtttaaa 10140 cgaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 10200 taataatggt ttctta 10216 // ID MN658784; SV 1; linear; other DNA; STD; SYN; 9810 BP. XX AC MN658784; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct QF2::SNAP25::HIVnes::syntaxin gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-9810 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; cb1cf4aeb220d013ffda663db6067a68. XX FH Key Location/Qualifiers FH FT source 1..9810 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,9703..9810) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT 5'UTR 7212..7232 FT /note="Syn21" FT CDS 7233..9416 FT /codon_start=1 FT /transl_table=11 FT /product="QF2::SNAP25::HIVnes::syntaxin" FT /protein_id="QGY73551.1" FT /translation="MPPKRKTLNAAAEANAHADGHADGNADGHVANTAASSNNARFADL FT TNIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCRAAREKCDGIQPACFPCVSQGRSCT FT YQASPKKRGVQTGYIRTLELALAWMFENVARSEDALHNLLVRDAGQGSALLVGKDSPAA FT ERLHARWATSRVNKSITRLLATASIMPHVMEQPLSTSINPVNDRFNGIPNPTPYNSDAA FT LDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPPYVDSTTNHPPY FT HSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPGCNFSDINTYEQ FT GKPIPNPLLGLDSTARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKA FT DSNKTRIDEANQRATKMLGSGSVSGLQLPPLERLTLQMTKDRLAALHAAQSDDEEETEV FT AVNVDGHDSYMDDFFAQVEEIRGMIDKVQDNVEEVKKKHSAILSAPQTDEKTKQELEDL FT MADIKKNANRVRGKLKGIEQNIEQEEQQNKSSADLRIRKTQHSTLSRKFVEVMTEYNRT FT QTDYRERCKGRIQRQLEITGRPTNDDELEKMLEEGNSSVFTQGIIMETQQAKQTLADIE FT ARHQDIMKLETSIKELHDMFMDMAMLVESQGEMIDRIEYHVEHAMDYVQTATQDTKKAL FT KYQSKARRKKIMILICLTVLGILAASYVSSYFM" FT misc_feature 7233..8252 FT /note="QF2" FT misc_feature 8253..8294 FT /note="1xV5 tag" FT misc_feature 8295..8492 FT /note="encodes amino acids 141-206 of human SNAP25" FT misc_feature 8505..8537 FT /note="HIV nuclear export signal (NES)" FT misc_feature 8541..9416 FT /note="Drosophila syntaxin" FT 3'UTR 9417..9702 FT /note="Hsp70" XX SQ Sequence 9810 BP; 2570 A; 2421 C; 2373 G; 2446 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg caacttaaaa aaaaaaatca aaatgccgcc taaacgcaag acactcaatg 7260 ccgctgccga agccaatgcc cacgctgatg gccatgctga tggcaatgct gatggtcatg 7320 tcgctaacac tgcagcaagc agcaacaacg cccgttttgc ggacttgacc aacattgaca 7380 cacccggcct cggccctacc acgacgacgt tacttgtcga gcccgctcgt tcgaaacgcc 7440 agagagtctc gagggcctgt gatcagtgtc gagctgcacg tgaaaagtgt gatggaatcc 7500 agccggcttg cttcccctgt gtgtcgcagg gccggtcgtg tacctaccag gccagtccca 7560 agaagcgagg agtccagacg ggctacatcc gcactctcga actggctctg gcttggatgt 7620 tcgagaacgt tgcccgcagc gaggacgccc tccacaatct tttggtccgt gatgctggcc 7680 agggcagcgc tctcctggtc ggcaaagact cgcctgctgc agaacgcctg catgcaagat 7740 gggcgacgag tcgagtcaac aaaagcatca cccgtcttct cgccactgca agtatcatgc 7800 cccatgtcat ggaacagccc ctgtcaacgt caataaatcc ggtcaatgat cggtttaatg 7860 gaataccgaa tcctaccccc tacaatagcg atgcggccct agacgcaatt actcaaacca 7920 atgactacgg atcagtcaat acccatggca ttctcagtac atatccacca cctgccaccc 7980 acttgaacga ggcctcggtg gcccttgcgc ctggaggtgc tcctcccaga ccacccccgc 8040 catacgttga tagtacaacg aaccatcctc cttaccatag taacctcgtc cccatggcca 8100 atttcggata ttcgactgta gactatgatg cgatggtgga tgatctggca tcgatcgagt 8160 acacggatgc ggttgacgtt gatccgcagt tcatgacgaa cctcgggttt gtcccagggt 8220 gtaattttag cgatatcaac acgtatgagc aaggtaagcc tatccctaac cctctccttg 8280 gtctcgattc tacggcccga gaaaatgaaa tggatgaaaa cctagagcag gtgagcggca 8340 tcatcgggaa cctccgtcac atggccctgg atatgggcaa tgagatcgat acacagaatc 8400 gccagatcga caggatcatg gagaaggctg attccaacaa aaccagaatt gatgaggcca 8460 accaacgtgc aacaaagatg ctgggaagtg gtagcgtaag cggtctgcag ctgcctccac 8520 tggaacgcct gaccctgcaa atgactaaag acagattagc cgctctccat gccgcccaat 8580 ccgatgacga ggaggagacg gaggtggccg tcaatgtgga tggccatgat tcctacatgg 8640 acgacttctt cgcccaggtg gaggagatcc gcgggatgat cgacaaggtg caggataacg 8700 tcgaggaggt aaagaagaag cactcggcca tcctgtccgc cccacaaacg gacgagaaga 8760 ccaagcaaga gctggaggat ctgatggccg acatcaagaa gaatgccaat cgcgttcgcg 8820 gcaagctcaa gggcatcgag cagaacatcg agcaggaaga gcagcagaat aagtcgtcgg 8880 cggatctgag aattcggaag acgcagcact cgacgttgtc acggaaattc gtcgaagtga 8940 tgaccgagta caatcgcacg cagaccgact accgagagcg ctgcaaggga aggatacagc 9000 gtcaactgga gattaccgga cgaccgacca acgacgatga gctggagaag atgctggagg 9060 agggcaactc gtctgtgttc acgcagggca tcatcatgga gacgcagcag gccaaacaga 9120 cgctggcgga cattgaggcc cgccaccagg acatcatgaa gctggagaca tcgatcaagg 9180 agctgcacga catgttcatg gacatggcca tgctggtgga gtcgcagggc gagatgatcg 9240 atcgcattga gtaccatgtg gagcacgcta tggactatgt gcagacggca actcaggaca 9300 ccaagaaggc gctcaagtac cagagtaaag cccgacgaaa gaagatcatg atactgatct 9360 gcctcactgt gctgggcatc ttagcggcct catatgttag cagttatttc atgtaacagg 9420 atctttgtga aggaacctta cttctgtggt gtgacagtcg actaaagcca aatagaaaat 9480 tattcagttc ctggcttaag tttttaaaag tgatattatt tatttggttg taaccaacca 9540 aaagaatgta aataactaat acataattat gttagtttta agttagcaac aaattgattt 9600 tagctatatt agctacttgg ttaataaata gaatatattt atttaaagat aattgcgttt 9660 ttattgtcag ggagtgagtt tgcttaaaaa ctcgtttaga tccatgtctg gatcgatctg 9720 gccggccgtt taaacgaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata 9780 ggttaatgtc atgataataa tggtttctta 9810 // ID MN658785; SV 1; linear; other DNA; STD; SYN; 9713 BP. XX AC MN658785; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct QF2::SNAP25::HIVnes::syntaxin gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-9713 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 0533793e32310da773563344f7ff7505. XX FH Key Location/Qualifiers FH FT source 1..9713 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7131,9606..9713) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7131 FT /note="from Hsp70" FT /regulatory_class="promoter" FT CDS 7136..9319 FT /codon_start=1 FT /transl_table=11 FT /product="QF2::SNAP25::HIVnes::syntaxin" FT /protein_id="QGY73552.1" FT /translation="MPPKRKTLNAAAEANAHADGHADGNADGHVANTAASSNNARFADL FT TNIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCRAAREKCDGIQPACFPCVSQGRSCT FT YQASPKKRGVQTGYIRTLELALAWMFENVARSEDALHNLLVRDAGQGSALLVGKDSPAA FT ERLHARWATSRVNKSITRLLATASIMPHVMEQPLSTSINPVNDRFNGIPNPTPYNSDAA FT LDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPPYVDSTTNHPPY FT HSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPGCNFSDINTYEQ FT GKPIPNPLLGLDSTARENEMDENLEQVSGIIGNLRHMALDMGNEIDTQNRQIDRIMEKA FT DSNKTRIDEANQRATKMLGSGSVSGLQLPPLERLTLQMTKDRLAALHAAQSDDEEETEV FT AVNVDGHDSYMDDFFAQVEEIRGMIDKVQDNVEEVKKKHSAILSAPQTDEKTKQELEDL FT MADIKKNANRVRGKLKGIEQNIEQEEQQNKSSADLRIRKTQHSTLSRKFVEVMTEYNRT FT QTDYRERCKGRIQRQLEITGRPTNDDELEKMLEEGNSSVFTQGIIMETQQAKQTLADIE FT ARHQDIMKLETSIKELHDMFMDMAMLVESQGEMIDRIEYHVEHAMDYVQTATQDTKKAL FT KYQSKARRKKIMILICLTVLGILAASYVSSYFM" FT misc_feature 7136..8155 FT /note="QF2" FT misc_feature 8156..8197 FT /note="1xV5 tag" FT misc_feature 8198..8395 FT /note="encodes amino acids 141-206 of human SNAP25" FT misc_feature 8408..8440 FT /note="HIV nuclear export signal (NES)" FT misc_feature 8444..9319 FT /note="Drosophila syntaxin" FT 3'UTR 9320..9605 FT /note="Hsp70B" XX SQ Sequence 9713 BP; 2532 A; 2398 C; 2360 G; 2423 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tcaaaatgcc 7140 gcctaaacgc aagacactca atgccgctgc cgaagccaat gcccacgctg atggccatgc 7200 tgatggcaat gctgatggtc atgtcgctaa cactgcagca agcagcaaca acgcccgttt 7260 tgcggacttg accaacattg acacacccgg cctcggccct accacgacga cgttacttgt 7320 cgagcccgct cgttcgaaac gccagagagt ctcgagggcc tgtgatcagt gtcgagctgc 7380 acgtgaaaag tgtgatggaa tccagccggc ttgcttcccc tgtgtgtcgc agggccggtc 7440 gtgtacctac caggccagtc ccaagaagcg aggagtccag acgggctaca tccgcactct 7500 cgaactggct ctggcttgga tgttcgagaa cgttgcccgc agcgaggacg ccctccacaa 7560 tcttttggtc cgtgatgctg gccagggcag cgctctcctg gtcggcaaag actcgcctgc 7620 tgcagaacgc ctgcatgcaa gatgggcgac gagtcgagtc aacaaaagca tcacccgtct 7680 tctcgccact gcaagtatca tgccccatgt catggaacag cccctgtcaa cgtcaataaa 7740 tccggtcaat gatcggttta atggaatacc gaatcctacc ccctacaata gcgatgcggc 7800 cctagacgca attactcaaa ccaatgacta cggatcagtc aatacccatg gcattctcag 7860 tacatatcca ccacctgcca cccacttgaa cgaggcctcg gtggcccttg cgcctggagg 7920 tgctcctccc agaccacccc cgccatacgt tgatagtaca acgaaccatc ctccttacca 7980 tagtaacctc gtccccatgg ccaatttcgg atattcgact gtagactatg atgcgatggt 8040 ggatgatctg gcatcgatcg agtacacgga tgcggttgac gttgatccgc agttcatgac 8100 gaacctcggg tttgtcccag ggtgtaattt tagcgatatc aacacgtatg agcaaggtaa 8160 gcctatccct aaccctctcc ttggtctcga ttctacggcc cgagaaaatg aaatggatga 8220 aaacctagag caggtgagcg gcatcatcgg gaacctccgt cacatggccc tggatatggg 8280 caatgagatc gatacacaga atcgccagat cgacaggatc atggagaagg ctgattccaa 8340 caaaaccaga attgatgagg ccaaccaacg tgcaacaaag atgctgggaa gtggtagcgt 8400 aagcggtctg cagctgcctc cactggaacg cctgaccctg caaatgacta aagacagatt 8460 agccgctctc catgccgccc aatccgatga cgaggaggag acggaggtgg ccgtcaatgt 8520 ggatggccat gattcctaca tggacgactt cttcgcccag gtggaggaga tccgcgggat 8580 gatcgacaag gtgcaggata acgtcgagga ggtaaagaag aagcactcgg ccatcctgtc 8640 cgccccacaa acggacgaga agaccaagca agagctggag gatctgatgg ccgacatcaa 8700 gaagaatgcc aatcgcgttc gcggcaagct caagggcatc gagcagaaca tcgagcagga 8760 agagcagcag aataagtcgt cggcggatct gagaattcgg aagacgcagc actcgacgtt 8820 gtcacggaaa ttcgtcgaag tgatgaccga gtacaatcgc acgcagaccg actaccgaga 8880 gcgctgcaag ggaaggatac agcgtcaact ggagattacc ggacgaccga ccaacgacga 8940 tgagctggag aagatgctgg aggagggcaa ctcgtctgtg ttcacgcagg gcatcatcat 9000 ggagacgcag caggccaaac agacgctggc ggacattgag gcccgccacc aggacatcat 9060 gaagctggag acatcgatca aggagctgca cgacatgttc atggacatgg ccatgctggt 9120 ggagtcgcag ggcgagatga tcgatcgcat tgagtaccat gtggagcacg ctatggacta 9180 tgtgcagacg gcaactcagg acaccaagaa ggcgctcaag taccagagta aagcccgacg 9240 aaagaagatc atgatactga tctgcctcac tgtgctgggc atcttagcgg cctcatatgt 9300 tagcagttat ttcatgtaac aggatctttg tgaaggaacc ttacttctgt ggtgtgacag 9360 tcgactaaag ccaaatagaa aattattcag ttcctggctt aagtttttaa aagtgatatt 9420 atttatttgg ttgtaaccaa ccaaaagaat gtaaataact aatacataat tatgttagtt 9480 ttaagttagc aacaaattga ttttagctat attagctact tggttaataa atagaatata 9540 tttatttaaa gataattgcg tttttattgt cagggagtga gtttgcttaa aaactcgttt 9600 agatccatgt ctggatcgat ctggccggcc gtttaaacga attcttgaag acgaaagggc 9660 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc tta 9713 // ID MN658786; SV 1; linear; other DNA; STD; SYN; 9964 BP. XX AC MN658786; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct QF2::syntaxin gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-9964 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; a9d652ac1e95c909c90cf6381ebe29a4. XX FH Key Location/Qualifiers FH FT source 1..9964 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,9169..9964) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT 5'UTR 7212..7232 FT /note="Syn21" FT CDS 7233..9167 FT /codon_start=1 FT /transl_table=11 FT /product="QF2::syntaxin" FT /protein_id="QGY73553.1" FT /translation="MPPKRKTLNAAAEANAHADGHADGNADGHVANTAASSNNARFADL FT TNIDTPGLGPTTTTLLVEPARSKRQRVSRACDQCRAAREKCDGIQPACFPCVSQGRSCT FT YQASPKKRGVQTGYIRTLELALAWMFENVARSEDALHNLLVRDAGQGSALLVGKDSPAA FT ERLHARWATSRVNKSITRLLATASIMPHVMEQPLSTSINPVNDRFNGIPNPTPYNSDAA FT LDAITQTNDYGSVNTHGILSTYPPPATHLNEASVALAPGGAPPRPPPPYVDSTTNHPPY FT HSNLVPMANFGYSTVDYDAMVDDLASIEYTDAVDVDPQFMTNLGFVPGCNFSDINTYEQ FT GKPIPNPLLGLDSTTKDRLAALHAAQSDDEEETEVAVNVDGHDSYMDDFFAQVEEIRGM FT IDKVQDNVEEVKKKHSAILSAPQTDEKTKQELEDLMADIKKNANRVRGKLKGIEQNIEQ FT EEQQNKSSADLRIRKTQHSTLSRKFVEVMTEYNRTQTDYRERCKGRIQRQLEITGRPTN FT DDELEKMLEEGNSSVFTQGIIMETQQAKQTLADIEARHQDIMKLETSIKELHDMFMDMA FT MLVESQGEMIDRIEYHVEHAMDYVQTATQDTKKALKYQSKARRKKIMILICLTVLGILA FT ASYVSSYFM" FT misc_feature 7233..8252 FT /note="QF2" FT misc_feature 8253..8294 FT /note="1xV5 tag" FT misc_feature 8295..9167 FT /note="Drosophila syntaxin" FT 3'UTR 9168..9867 FT /note="SV40" XX SQ Sequence 9964 BP; 2618 A; 2449 C; 2376 G; 2521 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg caacttaaaa aaaaaaatca aaatgccgcc taaacgcaag acactcaatg 7260 ccgctgccga agccaatgcc cacgctgatg gccatgctga tggcaatgct gatggtcatg 7320 tcgctaacac tgcagcaagc agcaacaacg cccgttttgc ggacttgacc aacattgaca 7380 cacccggcct cggccctacc acgacgacgt tacttgtcga gcccgctcgt tcgaaacgcc 7440 agagagtctc gagggcctgt gatcagtgtc gagctgcacg tgaaaagtgt gatggaatcc 7500 agccggcttg cttcccctgt gtgtcgcagg gccggtcgtg tacctaccag gccagtccca 7560 agaagcgagg agtccagacg ggctacatcc gcactctcga actggctctg gcttggatgt 7620 tcgagaacgt tgcccgcagc gaggacgccc tccacaatct tttggtccgt gatgctggcc 7680 agggcagcgc tctcctggtc ggcaaagact cgcctgctgc agaacgcctg catgcaagat 7740 gggcgacgag tcgagtcaac aaaagcatca cccgtcttct cgccactgca agtatcatgc 7800 cccatgtcat ggaacagccc ctgtcaacgt caataaatcc ggtcaatgat cggtttaatg 7860 gaataccgaa tcctaccccc tacaatagcg atgcggccct agacgcaatt actcaaacca 7920 atgactacgg atcagtcaat acccatggca ttctcagtac atatccacca cctgccaccc 7980 acttgaacga ggcctcggtg gcccttgcgc ctggaggtgc tcctcccaga ccacccccgc 8040 catacgttga tagtacaacg aaccatcctc cttaccatag taacctcgtc cccatggcca 8100 atttcggata ttcgactgta gactatgatg cgatggtgga tgatctggca tcgatcgagt 8160 acacggatgc ggttgacgtt gatccgcagt tcatgacgaa cctcgggttt gtcccagggt 8220 gtaattttag cgatatcaac acgtatgagc aaggtaagcc tatccctaac cctctccttg 8280 gtctcgattc tacgactaaa gacagattag ccgctctcca tgccgcccaa tccgatgacg 8340 aggaggagac ggaggtggcc gtcaatgtgg atggccatga ttcctacatg gacgacttct 8400 tcgcccaggt ggaggagatc cgcgggatga tcgacaaggt gcaggataac gtcgaggagg 8460 taaagaagaa gcactcggcc atcctgtccg ccccacaaac ggacgagaag accaagcaag 8520 agctggagga tctgatggcc gacatcaaga agaatgccaa tcgcgttcgc ggcaagctca 8580 agggcatcga gcagaacatc gagcaggaag agcagcagaa taagtcgtcg gcggatctga 8640 gaattcggaa gacgcagcac tcgacgttgt cacggaaatt cgtcgaagtg atgaccgagt 8700 acaatcgcac gcagaccgac taccgagagc gctgcaaggg aaggatacag cgtcaactgg 8760 agattaccgg acgaccgacc aacgacgatg agctggagaa gatgctggag gagggcaact 8820 cgtctgtgtt cacgcagggc atcatcatgg agacgcagca ggccaaacag acgctggcgg 8880 acattgaggc ccgccaccag gacatcatga agctggagac atcgatcaag gagctgcacg 8940 acatgttcat ggacatggcc atgctggtgg agtcgcaggg cgagatgatc gatcgcattg 9000 agtaccatgt ggagcacgct atggactatg tgcagacggc aactcaggac accaagaagg 9060 cgctcaagta ccagagtaaa gcccgacgaa agaagatcat gatactgatc tgcctcactg 9120 tgctgggcat cttagcggcc tcatatgtta gcagttattt catgtaacag gatctttgtg 9180 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc 9240 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt 9300 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt 9360 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct 9420 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt 9480 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc 9540 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa 9600 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt 9660 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 9720 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 9780 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 9840 catcaatgta tcttatcatg tctggatcga tctggccggc cgtttaaacg aattcttgaa 9900 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 9960 ctta 9964 // ID MN658787; SV 1; linear; other DNA; STD; SYN; 12952 BP. XX AC MN658787; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct Flp1 and TEV::CD2 genes, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-12952 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; ac67fdcd19162226a3917c26078d5883. XX FH Key Location/Qualifiers FH FT source 1..12952 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,12157..12952) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="LexAop2x13" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT misc_feature 7212..7242 FT /note="RSRT" FT 5'UTR 7243..7263 FT /note="Syn21" FT CDS 7264..8541 FT /codon_start=1 FT /transl_table=11 FT /product="Flp1" FT /protein_id="QGY73555.1" FT /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI FT THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI FT IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN FT SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET FT KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR FT SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR FT TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR FT YPAWNGIISQEVLDYLSSYINRRIGS" FT 3'UTR 8547..8789 FT /note="Hsp70B" FT 3'UTR 8868..9564 FT /note="SV40" FT misc_feature 9566..9596 FT /note="RSRT" FT 5'UTR 9615..9635 FT /note="Syn21" FT CDS 9636..11489 FT /codon_start=1 FT /transl_table=11 FT /product="TEV::CD2" FT /protein_id="QGY73554.1" FT /translation="MKFLVNVALVFMVVYISYIYAAPWMESLFKGPRDYNPISSTICHL FT TNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGR FT DMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIF FT WKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQW FT VSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLMNELVYSQEQKLISEEDLNEMDC FT RDSGTVWGALGHGINLNIPNFQMTDDIDEVRWERGSTLVAEFKRKMKPFLKSGAFEILA FT NGDLKIKNLTRDDSGTYNVTVYSTNGTRILDKALDLRILEMVSKPMIYWECSNATLTCE FT VLEGTDVELKLYQGKEHLRSLRQKTMSYQWTNLRAPFKCKAVNRVSQESEMEVVNCPEK FT GLPLYLIVGVSAGGLLLVFFGALFIFCICKRKKRNRRRKGEELEIKASRMSTVERGPKP FT HSTQASAPASQNPVASQAPPPPGHHLQTPGHRPLPPSHRNREHQPKKRPPPSGTQVHQQ FT KGPPLPRPRVQPKPPCGSGDVSLPPPNENANSFCYENEVAL" FT misc_feature 9636..10433 FT /note="Drosophila codon optimised TEV" FT sig_peptide 9636..9701 FT /note="from honeybee melittin (MBP)" FT misc_feature 10434..10472 FT /note="1xMyc tag" FT misc_feature 10473..11444 FT /note="CD2 from rat" FT misc_feature 11022..11090 FT /note="transmembrane domain" FT misc_feature 11448..11486 FT /note="ER export signal from Kir2.1" FT 3'UTR 11490..12155 FT /note="P10" FT 3'UTR 12159..12855 FT /note="SV40" XX SQ Sequence 12952 BP; 3543 A; 3055 C; 2982 G; 3372 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg cttgatgaaa gaataacgta ttctttcatc aaaacttaaa aaaaaaaatc 7260 aaaatgccgc agtttgatat cctctgcaag accccaccaa aggtgttggt gcgtcaattc 7320 gtggagcgat ttgagaggcc gtcgggtgag aagatcgccc tgtgcgctgc cgagttgacc 7380 tatttgtgtt ggatgatcac tcataatggc accgcgatta agcgcgctac ctttatgagc 7440 tataacacta tcattagcaa ttccctgtcc tttgacatag taaacaagtc cctgcagttt 7500 aaatacaaga ctcagaaggc cactatattg gaggcttcgc tgaaaaagtt gatcccggca 7560 tgggagttca cgatcatccc atactacggt cagaaacacc agagcgatat taccgatatt 7620 gtaagcagcc tccagctgca gtttgagtcc agcgaagagg ctgataaggg taatagtcac 7680 agcaaaaaga tgctgaaggc actgctgtcc gagggcgaaa gcatctggga gattactgag 7740 aaaatcctga actcgtttga gtacaccagc cgattcacca agacgaagac cctgtaccag 7800 ttcctctttt tggcaacctt catcaattgt ggtcgcttca gtgacatcaa aaacgtggac 7860 cctaaatcgt tcaagctggt gcagaataag tacctgggtg ttatcatcca gtgcttggtt 7920 accgagacta agacctccgt gtccaggcac atttatttct tctccgcaag gggtcgcatc 7980 gatccgctgg tatacttgga tgagttcctt cggaatagtg aaccagtctt gaagcgcgtg 8040 aacaggacgg gcaattccag tagcaacaag caagagtacc agctgctgaa ggataatctt 8100 gttcggtcgt acaacaaagc cttgaagaaa aatgcgccat actcgatctt tgcaatcaag 8160 aatggtccca agagtcatat tggccggcac ctgatgacca gtttcttgtc catgaagggt 8220 ctgactgagc tcacaaacgt cgttggcaac tggtcggaca agcgagcgag tgccgtcgcc 8280 cgcacgacat acacccacca gatcacggcc ataccagatc actatttcgc gctggtgtcg 8340 cgttattatg cctatgatcc catcagcaag gagatgatcg cgctgaagga cgaaaccaat 8400 ccaatcgagg agtggcagca tatcgagcaa cttaagggaa gcgctgaggg tagcatccgt 8460 taccccgcct ggaacggcat catcagccag gaggttctgg attacctgag ctcctacatc 8520 aatcgccgta ttggatccta agaattgact aaagccaaat agaaaattat tcagttcctg 8580 gcttaagttt ttaaaagtga tattatttat ttggttgtaa ccaaccaaaa gaatgtaaat 8640 aactaataca taattatgtt agttttaagt tagcaacaaa ttgattttag ctatattagc 8700 tacttggtta ataaatagaa tatatttatt taaagataat tgcgttttta ttgtcaggga 8760 gtgagtttgc ttaaaaactc gtttagatcc actagttcta gttgcggccg gctaagaatt 8820 cgatatcaag cttatcgata ccgtcgacct cgagggtacc tctagaggat ctttgtgaag 8880 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct 8940 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt 9000 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat 9060 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac 9120 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct 9180 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt 9240 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat 9300 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt 9360 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 9420 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 9480 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 9540 caatgtatct tatcatgtct ggatcttgat gaaagaataa cgtattcttt catcaacaaa 9600 tccgccacga atcaaactta aaaaaaaaaa tcaaaatgaa gttcctcgtg aacgtggccc 9660 tggtgttcat ggtggtgtac atcagctaca tctatgccgc cccatggatg gagagcctgt 9720 tcaagggccc acgcgattac aaccccatca gcagcaccat ctgccacctg accaacgagt 9780 ccgatggcca caccaccagc ctgtacggca ttggcttcgg ccccttcatc atcaccaaca 9840 agcacctgtt ccgccgcaac aacggcaccc tgctggtgca gagtctgcat ggcgtgttca 9900 aagtgaagaa taccaccacc ctgcagcagc atctgatcga tggccgcgat atgatcatca 9960 tccgcatgcc caaggatttc ccgcccttcc cgcagaagct gaagttccgc gagccacagc 10020 gcgaggagcg catttgcctc gtgaccacca acttccagac caagagcatg agcagcatgg 10080 tgtccgatac cagctgcacc ttccccagct ccgatggcat cttctggaag cactggattc 10140 agaccaagga tggccagtgc ggcagccccc tggtgtccac ccgcgacgga ttcatcgtgg 10200 gcattcacag cgccagcaac ttcacgaata ccaacaacta cttcaccagc gtgcccaaga 10260 acttcatgga gctgctgacc aaccaggagg cccagcagtg ggtgtccgga tggcgcctga 10320 atgccgatag cgtgctgtgg ggcggacaca aggtgttcat ggtcaagccc gaggagccct 10380 tccagcccgt gaaggaggcc acccagctga tgaacgagct ggtgtactcc caggagcaaa 10440 agctcatttc tgaagaggac ttgaatgaaa tggactgcag agacagtggg accgtctggg 10500 gtgccctggg tcatggcatc aacctgaaca tccctaactt tcaaatgact gatgatattg 10560 atgaggtgcg atgggagagg gggagcaccc tggttgccga gtttaaaagg aagatgaagc 10620 cttttttgaa atcgggagca tttgagatct tagcaaatgg agacttgaag ataaagaatc 10680 tgacaagaga tgacagtggc acctataatg taacggtata cagcacaaat gggacacgta 10740 tcctggacaa ggcactggac ttgaggatcc tagagatggt ctcaaagccg atgatctact 10800 gggagtgcag caacgcaacc ctgacctgtg aggtcttgga aggaacagat gttgaactaa 10860 agctgtacca aggaaaggag catctcagga gcctccgtca gaagaccatg agttaccagt 10920 ggaccaacct gagagcaccg tttaagtgca aggcggtaaa cagggtcagc caggagtctg 10980 agatggaagt tgtcaactgt ccagagaaag gtctgcccct ctatctcata gtgggggtca 11040 gtgcaggagg actcctcttg gtgttctttg gggcgctgtt tattttctgt atctgcaaga 11100 ggaaaaaacg gaacaggagg agaaaaggtg aagagctgga gataaaagct tccagaatga 11160 gcactgtgga aaggggaccc aagccacact caacccaggc ctcagcacca gcatcacaga 11220 atccagtggc ttcccaagct cctcctccac ctggccatca tctccagaca cctggccatc 11280 gtcccctgcc tccaagccac cgtaaccgtg agcaccagcc gaagaagaga cctcctccat 11340 caggcacaca ggttcaccag cagaaaggcc ctcctttgcc cagaccccga gttcagccaa 11400 agcctccctg tgggagtgga gatgtttcgt tgccaccccc taatgagaac gcaaacagct 11460 tttgctatga aaatgaagtt gccctctaaa tgaatcgttt ttaaaataac aaatcaattg 11520 ttttataata ttcgtacgat tctttgatta tgtaataaaa tgtgatcatt aggaagatta 11580 cgaaaaatat aaaaaatatg agttctgtgt gtataacaaa tgctgtaaac gccacaattg 11640 tgtttgttgc aaataaaccc atgattattt gattaaaatt gttgttttct ttgttcatag 11700 acaatagtgt gttttgccta aacgtgtact gcataaactc catgcgagtg tatagcgagc 11760 tagtggctaa cgcttgcccc accaaagtag attcgtcaaa atcctcaatt tcatcaccct 11820 cctccaagtt taacatttgg ccgtcggaat taacttctaa agatgccaca taatctaata 11880 aatgaaatag agattcaaac gtggcgtcat cgtccgtttc gaccatttcc gaaaagaact 11940 cgggcataaa ctctatgatt tctctggacg tggtgttgtc gaaactctca aagtacgcag 12000 tcaggaacgt gcgcgacatg tcgtcgggaa actcgcgcgg aaacatgttg ttgtaaccga 12060 acgggtccca tagcgccaaa accaaatctg ccagcgtcaa tagaatgagc acgatgccga 12120 caatggagct ggcttggata gcgattcgag ttaactagga tctttgtgaa ggaaccttac 12180 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat 12240 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag 12300 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac 12360 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat 12420 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg 12480 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac 12540 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata tttgatgtat 12600 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt 12660 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 12720 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 12780 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 12840 ttatcatgtc tggatcgatc tggccggccg tttaaacgaa ttcttgaaga cgaaagggcc 12900 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct ta 12952 // ID MN658788; SV 1; linear; other DNA; STD; SYN; 12952 BP. XX AC MN658788; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct Flp1 and TEVt173v::CD2 genes, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-12952 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; c9201c7c07f20a9f9011669753684294. XX FH Key Location/Qualifiers FH FT source 1..12952 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,12157..12952) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT misc_feature 7212..7242 FT /note="RSRT" FT 5'UTR 7243..7263 FT /note="Syn21" FT CDS 7264..8541 FT /codon_start=1 FT /transl_table=11 FT /product="Flp1" FT /protein_id="QGY73556.1" FT /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI FT THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI FT IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN FT SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET FT KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR FT SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR FT TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR FT YPAWNGIISQEVLDYLSSYINRRIGS" FT 3'UTR 8547..8789 FT /note="Hsp70B" FT 3'UTR 8868..9564 FT /note="SV40" FT misc_feature 9566..9596 FT /note="RSRT" FT 5'UTR 9615..9635 FT /note="Syn21" FT CDS 9636..11489 FT /codon_start=1 FT /transl_table=11 FT /product="TEVt173v::CD2" FT /protein_id="QGY73557.1" FT /translation="MKFLVNVALVFMVVYISYIYAAPWMESLFKGPRDYNPISSTICHL FT TNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGR FT DMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIF FT WKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFVNTNNYFTSVPKNFMELLTNQEAQQW FT VSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLMNELVYSQEQKLISEEDLNEMDC FT RDSGTVWGALGHGINLNIPNFQMTDDIDEVRWERGSTLVAEFKRKMKPFLKSGAFEILA FT NGDLKIKNLTRDDSGTYNVTVYSTNGTRILDKALDLRILEMVSKPMIYWECSNATLTCE FT VLEGTDVELKLYQGKEHLRSLRQKTMSYQWTNLRAPFKCKAVNRVSQESEMEVVNCPEK FT GLPLYLIVGVSAGGLLLVFFGALFIFCICKRKKRNRRRKGEELEIKASRMSTVERGPKP FT HSTQASAPASQNPVASQAPPPPGHHLQTPGHRPLPPSHRNREHQPKKRPPPSGTQVHQQ FT KGPPLPRPRVQPKPPCGSGDVSLPPPNENANSFCYENEVAL" FT misc_feature 9636..10433 FT /note="Drosophila codon optimised TEVt173v" FT sig_peptide 9636..9701 FT /note="from honeybee melittin (MBP)" FT misc_feature 10224..10226 FT /note="T173V mutation - removes glycosylation site" FT misc_feature 10434..10472 FT /note="1xMyc tag" FT misc_feature 10473..11444 FT /note="CD2 from rat" FT misc_feature 11022..11090 FT /note="transmembrane domain" FT misc_feature 11448..11486 FT /note="ER export signal from Kir2.1" FT 3'UTR 11490..12155 FT /note="P10" FT 3'UTR 12159..12855 FT /note="SV40" XX SQ Sequence 12952 BP; 3542 A; 3054 C; 2983 G; 3373 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg cttgatgaaa gaataacgta ttctttcatc aaaacttaaa aaaaaaaatc 7260 aaaatgccgc agtttgatat cctctgcaag accccaccaa aggtgttggt gcgtcaattc 7320 gtggagcgat ttgagaggcc gtcgggtgag aagatcgccc tgtgcgctgc cgagttgacc 7380 tatttgtgtt ggatgatcac tcataatggc accgcgatta agcgcgctac ctttatgagc 7440 tataacacta tcattagcaa ttccctgtcc tttgacatag taaacaagtc cctgcagttt 7500 aaatacaaga ctcagaaggc cactatattg gaggcttcgc tgaaaaagtt gatcccggca 7560 tgggagttca cgatcatccc atactacggt cagaaacacc agagcgatat taccgatatt 7620 gtaagcagcc tccagctgca gtttgagtcc agcgaagagg ctgataaggg taatagtcac 7680 agcaaaaaga tgctgaaggc actgctgtcc gagggcgaaa gcatctggga gattactgag 7740 aaaatcctga actcgtttga gtacaccagc cgattcacca agacgaagac cctgtaccag 7800 ttcctctttt tggcaacctt catcaattgt ggtcgcttca gtgacatcaa aaacgtggac 7860 cctaaatcgt tcaagctggt gcagaataag tacctgggtg ttatcatcca gtgcttggtt 7920 accgagacta agacctccgt gtccaggcac atttatttct tctccgcaag gggtcgcatc 7980 gatccgctgg tatacttgga tgagttcctt cggaatagtg aaccagtctt gaagcgcgtg 8040 aacaggacgg gcaattccag tagcaacaag caagagtacc agctgctgaa ggataatctt 8100 gttcggtcgt acaacaaagc cttgaagaaa aatgcgccat actcgatctt tgcaatcaag 8160 aatggtccca agagtcatat tggccggcac ctgatgacca gtttcttgtc catgaagggt 8220 ctgactgagc tcacaaacgt cgttggcaac tggtcggaca agcgagcgag tgccgtcgcc 8280 cgcacgacat acacccacca gatcacggcc ataccagatc actatttcgc gctggtgtcg 8340 cgttattatg cctatgatcc catcagcaag gagatgatcg cgctgaagga cgaaaccaat 8400 ccaatcgagg agtggcagca tatcgagcaa cttaagggaa gcgctgaggg tagcatccgt 8460 taccccgcct ggaacggcat catcagccag gaggttctgg attacctgag ctcctacatc 8520 aatcgccgta ttggatccta agaattgact aaagccaaat agaaaattat tcagttcctg 8580 gcttaagttt ttaaaagtga tattatttat ttggttgtaa ccaaccaaaa gaatgtaaat 8640 aactaataca taattatgtt agttttaagt tagcaacaaa ttgattttag ctatattagc 8700 tacttggtta ataaatagaa tatatttatt taaagataat tgcgttttta ttgtcaggga 8760 gtgagtttgc ttaaaaactc gtttagatcc actagttcta gttgcggccg gctaagaatt 8820 cgatatcaag cttatcgata ccgtcgacct cgagggtacc tctagaggat ctttgtgaag 8880 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct 8940 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt 9000 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat 9060 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac 9120 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct 9180 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt 9240 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat 9300 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt 9360 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 9420 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 9480 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 9540 caatgtatct tatcatgtct ggatcttgat gaaagaataa cgtattcttt catcaacaaa 9600 tccgccacga atcaaactta aaaaaaaaaa tcaaaatgaa gttcctcgtg aacgtggccc 9660 tggtgttcat ggtggtgtac atcagctaca tctatgccgc cccatggatg gagagcctgt 9720 tcaagggccc acgcgattac aaccccatca gcagcaccat ctgccacctg accaacgagt 9780 ccgatggcca caccaccagc ctgtacggca ttggcttcgg ccccttcatc atcaccaaca 9840 agcacctgtt ccgccgcaac aacggcaccc tgctggtgca gagtctgcat ggcgtgttca 9900 aagtgaagaa taccaccacc ctgcagcagc atctgatcga tggccgcgat atgatcatca 9960 tccgcatgcc caaggatttc ccgcccttcc cgcagaagct gaagttccgc gagccacagc 10020 gcgaggagcg catttgcctc gtgaccacca acttccagac caagagcatg agcagcatgg 10080 tgtccgatac cagctgcacc ttccccagct ccgatggcat cttctggaag cactggattc 10140 agaccaagga tggccagtgc ggcagccccc tggtgtccac ccgcgacgga ttcatcgtgg 10200 gcattcacag cgccagcaac ttcgtgaata ccaacaacta cttcaccagc gtgcccaaga 10260 acttcatgga gctgctgacc aaccaggagg cccagcagtg ggtgtccgga tggcgcctga 10320 atgccgatag cgtgctgtgg ggcggacaca aggtgttcat ggtcaagccc gaggagccct 10380 tccagcccgt gaaggaggcc acccagctga tgaacgagct ggtgtactcc caggagcaaa 10440 agctcatttc tgaagaggac ttgaatgaaa tggactgcag agacagtggg accgtctggg 10500 gtgccctggg tcatggcatc aacctgaaca tccctaactt tcaaatgact gatgatattg 10560 atgaggtgcg atgggagagg gggagcaccc tggttgccga gtttaaaagg aagatgaagc 10620 cttttttgaa atcgggagca tttgagatct tagcaaatgg agacttgaag ataaagaatc 10680 tgacaagaga tgacagtggc acctataatg taacggtata cagcacaaat gggacacgta 10740 tcctggacaa ggcactggac ttgaggatcc tagagatggt ctcaaagccg atgatctact 10800 gggagtgcag caacgcaacc ctgacctgtg aggtcttgga aggaacagat gttgaactaa 10860 agctgtacca aggaaaggag catctcagga gcctccgtca gaagaccatg agttaccagt 10920 ggaccaacct gagagcaccg tttaagtgca aggcggtaaa cagggtcagc caggagtctg 10980 agatggaagt tgtcaactgt ccagagaaag gtctgcccct ctatctcata gtgggggtca 11040 gtgcaggagg actcctcttg gtgttctttg gggcgctgtt tattttctgt atctgcaaga 11100 ggaaaaaacg gaacaggagg agaaaaggtg aagagctgga gataaaagct tccagaatga 11160 gcactgtgga aaggggaccc aagccacact caacccaggc ctcagcacca gcatcacaga 11220 atccagtggc ttcccaagct cctcctccac ctggccatca tctccagaca cctggccatc 11280 gtcccctgcc tccaagccac cgtaaccgtg agcaccagcc gaagaagaga cctcctccat 11340 caggcacaca ggttcaccag cagaaaggcc ctcctttgcc cagaccccga gttcagccaa 11400 agcctccctg tgggagtgga gatgtttcgt tgccaccccc taatgagaac gcaaacagct 11460 tttgctatga aaatgaagtt gccctctaaa tgaatcgttt ttaaaataac aaatcaattg 11520 ttttataata ttcgtacgat tctttgatta tgtaataaaa tgtgatcatt aggaagatta 11580 cgaaaaatat aaaaaatatg agttctgtgt gtataacaaa tgctgtaaac gccacaattg 11640 tgtttgttgc aaataaaccc atgattattt gattaaaatt gttgttttct ttgttcatag 11700 acaatagtgt gttttgccta aacgtgtact gcataaactc catgcgagtg tatagcgagc 11760 tagtggctaa cgcttgcccc accaaagtag attcgtcaaa atcctcaatt tcatcaccct 11820 cctccaagtt taacatttgg ccgtcggaat taacttctaa agatgccaca taatctaata 11880 aatgaaatag agattcaaac gtggcgtcat cgtccgtttc gaccatttcc gaaaagaact 11940 cgggcataaa ctctatgatt tctctggacg tggtgttgtc gaaactctca aagtacgcag 12000 tcaggaacgt gcgcgacatg tcgtcgggaa actcgcgcgg aaacatgttg ttgtaaccga 12060 acgggtccca tagcgccaaa accaaatctg ccagcgtcaa tagaatgagc acgatgccga 12120 caatggagct ggcttggata gcgattcgag ttaactagga tctttgtgaa ggaaccttac 12180 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat 12240 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag 12300 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac 12360 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat 12420 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg 12480 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac 12540 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata tttgatgtat 12600 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt 12660 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt 12720 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac 12780 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc 12840 ttatcatgtc tggatcgatc tggccggccg tttaaacgaa ttcttgaaga cgaaagggcc 12900 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct ta 12952 // ID MN658789; SV 1; linear; other DNA; STD; SYN; 12964 BP. XX AC MN658789; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct Flp1 and TEV3xmut::CD2 genes, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-12964 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 86b2e92e509d6f26fb025a4af0df44fd. XX FH Key Location/Qualifiers FH FT source 1..12964 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,12169..12964) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT misc_feature 7212..7242 FT /note="RSRT" FT 5'UTR 7243..7263 FT /note="Syn21" FT CDS 7264..8541 FT /codon_start=1 FT /transl_table=11 FT /product="Flp1" FT /protein_id="QGY73559.1" FT /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI FT THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI FT IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN FT SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET FT KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR FT SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR FT TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR FT YPAWNGIISQEVLDYLSSYINRRIGS" FT 3'UTR 8547..8789 FT /note="Hsp70B" FT 3'UTR 8868..9564 FT /note="SV40" FT misc_feature 9566..9596 FT /note="RSRT" FT 5'UTR 9615..9635 FT /note="Syn21" FT CDS 9636..11501 FT /codon_start=1 FT /transl_table=11 FT /product="TEV3xmut::CD2" FT /protein_id="QGY73558.1" FT /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTESLFKGPRDYNPISST FT ICHLTQESDGHTTSLYGIGFGPFIITNKHLFRRNNGVLLVQSLHGVFKVKNTTTLQQHL FT IDGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSS FT DGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFVNTNNYFTSVPKNFMELLTNQE FT AQQWVSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLMNELVYSQEQKLISEEDLN FT EMDCRDSGTVWGALGHGINLNIPNFQMTDDIDEVRWERGSTLVAEFKRKMKPFLKSGAF FT EILANGDLKIKNLTRDDSGTYNVTVYSTNGTRILDKALDLRILEMVSKPMIYWECSNAT FT LTCEVLEGTDVELKLYQGKEHLRSLRQKTMSYQWTNLRAPFKCKAVNRVSQESEMEVVN FT CPEKGLPLYLIVGVSAGGLLLVFFGALFIFCICKRKKRNRRRKGEELEIKASRMSTVER FT GPKPHSTQASAPASQNPVASQAPPPPGHHLQTPGHRPLPPSHRNREHQPKKRPPPSGTQ FT VHQQKGPPLPRPRVQPKPPCGSGDVSLPPPNENANSFCYENEVAL" FT misc_feature 9636..10445 FT /note="TEV N23Q, T54V, T173V - Drosophila codon optimised" FT sig_peptide 9636..9722 FT /note="PAT3 signal peptide" FT misc_feature 9786..9788 FT /note="N23Q mutation" FT misc_feature 9879..9881 FT /note="T54V mutation" FT misc_feature 10236..10238 FT /note="T173V mutation" FT misc_feature 10446..10484 FT /note="1xMyc tag" FT misc_feature 10485..11456 FT /note="CD2 from rat" FT misc_feature 11034..11102 FT /note="transmembrane domain" FT misc_feature 11460..11498 FT /note="ER export signal from Kir2.1" FT 3'UTR 11502..12167 FT /note="P10" FT 3'UTR 12171..12867 FT /note="SV40" XX SQ Sequence 12964 BP; 3516 A; 3111 C; 2973 G; 3364 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg cttgatgaaa gaataacgta ttctttcatc aaaacttaaa aaaaaaaatc 7260 aaaatgccgc agtttgatat cctctgcaag accccaccaa aggtgttggt gcgtcaattc 7320 gtggagcgat ttgagaggcc gtcgggtgag aagatcgccc tgtgcgctgc cgagttgacc 7380 tatttgtgtt ggatgatcac tcataatggc accgcgatta agcgcgctac ctttatgagc 7440 tataacacta tcattagcaa ttccctgtcc tttgacatag taaacaagtc cctgcagttt 7500 aaatacaaga ctcagaaggc cactatattg gaggcttcgc tgaaaaagtt gatcccggca 7560 tgggagttca cgatcatccc atactacggt cagaaacacc agagcgatat taccgatatt 7620 gtaagcagcc tccagctgca gtttgagtcc agcgaagagg ctgataaggg taatagtcac 7680 agcaaaaaga tgctgaaggc actgctgtcc gagggcgaaa gcatctggga gattactgag 7740 aaaatcctga actcgtttga gtacaccagc cgattcacca agacgaagac cctgtaccag 7800 ttcctctttt tggcaacctt catcaattgt ggtcgcttca gtgacatcaa aaacgtggac 7860 cctaaatcgt tcaagctggt gcagaataag tacctgggtg ttatcatcca gtgcttggtt 7920 accgagacta agacctccgt gtccaggcac atttatttct tctccgcaag gggtcgcatc 7980 gatccgctgg tatacttgga tgagttcctt cggaatagtg aaccagtctt gaagcgcgtg 8040 aacaggacgg gcaattccag tagcaacaag caagagtacc agctgctgaa ggataatctt 8100 gttcggtcgt acaacaaagc cttgaagaaa aatgcgccat actcgatctt tgcaatcaag 8160 aatggtccca agagtcatat tggccggcac ctgatgacca gtttcttgtc catgaagggt 8220 ctgactgagc tcacaaacgt cgttggcaac tggtcggaca agcgagcgag tgccgtcgcc 8280 cgcacgacat acacccacca gatcacggcc ataccagatc actatttcgc gctggtgtcg 8340 cgttattatg cctatgatcc catcagcaag gagatgatcg cgctgaagga cgaaaccaat 8400 ccaatcgagg agtggcagca tatcgagcaa cttaagggaa gcgctgaggg tagcatccgt 8460 taccccgcct ggaacggcat catcagccag gaggttctgg attacctgag ctcctacatc 8520 aatcgccgta ttggatccta agaattgact aaagccaaat agaaaattat tcagttcctg 8580 gcttaagttt ttaaaagtga tattatttat ttggttgtaa ccaaccaaaa gaatgtaaat 8640 aactaataca taattatgtt agttttaagt tagcaacaaa ttgattttag ctatattagc 8700 tacttggtta ataaatagaa tatatttatt taaagataat tgcgttttta ttgtcaggga 8760 gtgagtttgc ttaaaaactc gtttagatcc actagttcta gttgcggccg gctaagaatt 8820 cgatatcaag cttatcgata ccgtcgacct cgagggtacc tctagaggat ctttgtgaag 8880 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct 8940 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt 9000 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat 9060 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac 9120 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct 9180 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt 9240 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat 9300 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt 9360 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat 9420 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac 9480 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat 9540 caatgtatct tatcatgtct ggatcttgat gaaagaataa cgtattcttt catcaacaaa 9600 tccgccacga atcaaactta aaaaaaaaaa tcaaaatgcc accctccacc tccctgctgc 9660 tgctggccgc cctgctgccc ttcgccctgc ccgcctccga ctggaagacc ggcgaggtga 9720 ccgagtccct gttcaagggc ccccgcgact acaaccccat ctcctccacc atctgccacc 9780 tgacccagga gtccgacggc cacaccacct ccctgtacgg catcggcttc ggccccttca 9840 tcatcaccaa caagcacctg ttccgccgca acaacggcgt gctgctggtg cagtccctgc 9900 acggcgtgtt caaggtgaag aacaccacca ccctgcagca gcacctgatc gacggccgcg 9960 acatgatcat catccgcatg cccaaggact tcccaccctt cccccagaag ctgaagttcc 10020 gcgagcccca gcgcgaggag cgcatctgcc tggtgaccac caacttccag accaagtcca 10080 tgtcctccat ggtgtccgac acctcctgca ccttcccctc ctccgacggc atcttctgga 10140 agcactggat ccagaccaag gacggccagt gcggctcccc actggtgtcc acccgcgacg 10200 gcttcatcgt gggcatccac tccgcctcca acttcgtgaa caccaacaac tacttcacct 10260 ccgtgcccaa gaacttcatg gagctgctga ccaaccagga ggcccagcag tgggtgtccg 10320 gctggcgcct gaacgccgac tccgtgctgt ggggcggcca caaggtgttc atggtgaagc 10380 ccgaggagcc cttccagccc gtgaaggagg ccacccagct gatgaacgag ctggtgtact 10440 cccaggagca aaagctcatt tctgaagagg acttgaatga aatggactgc agagacagtg 10500 ggaccgtctg gggtgccctg ggtcatggca tcaacctgaa catccctaac tttcaaatga 10560 ctgatgatat tgatgaggtg cgatgggaga gggggagcac cctggttgcc gagtttaaaa 10620 ggaagatgaa gccttttttg aaatcgggag catttgagat cttagcaaat ggagacttga 10680 agataaagaa tctgacaaga gatgacagtg gcacctataa tgtaacggta tacagcacaa 10740 atgggacacg tatcctggac aaggcactgg acttgaggat cctagagatg gtctcaaagc 10800 cgatgatcta ctgggagtgc agcaacgcaa ccctgacctg tgaggtcttg gaaggaacag 10860 atgttgaact aaagctgtac caaggaaagg agcatctcag gagcctccgt cagaagacca 10920 tgagttacca gtggaccaac ctgagagcac cgtttaagtg caaggcggta aacagggtca 10980 gccaggagtc tgagatggaa gttgtcaact gtccagagaa aggtctgccc ctctatctca 11040 tagtgggggt cagtgcagga ggactcctct tggtgttctt tggggcgctg tttattttct 11100 gtatctgcaa gaggaaaaaa cggaacagga ggagaaaagg tgaagagctg gagataaaag 11160 cttccagaat gagcactgtg gaaaggggac ccaagccaca ctcaacccag gcctcagcac 11220 cagcatcaca gaatccagtg gcttcccaag ctcctcctcc acctggccat catctccaga 11280 cacctggcca tcgtcccctg cctccaagcc accgtaaccg tgagcaccag ccgaagaaga 11340 gacctcctcc atcaggcaca caggttcacc agcagaaagg ccctcctttg cccagacccc 11400 gagttcagcc aaagcctccc tgtgggagtg gagatgtttc gttgccaccc cctaatgaga 11460 acgcaaacag cttttgctat gaaaatgaag ttgccctcta aatgaatcgt ttttaaaata 11520 acaaatcaat tgttttataa tattcgtacg attctttgat tatgtaataa aatgtgatca 11580 ttaggaagat tacgaaaaat ataaaaaata tgagttctgt gtgtataaca aatgctgtaa 11640 acgccacaat tgtgtttgtt gcaaataaac ccatgattat ttgattaaaa ttgttgtttt 11700 ctttgttcat agacaatagt gtgttttgcc taaacgtgta ctgcataaac tccatgcgag 11760 tgtatagcga gctagtggct aacgcttgcc ccaccaaagt agattcgtca aaatcctcaa 11820 tttcatcacc ctcctccaag tttaacattt ggccgtcgga attaacttct aaagatgcca 11880 cataatctaa taaatgaaat agagattcaa acgtggcgtc atcgtccgtt tcgaccattt 11940 ccgaaaagaa ctcgggcata aactctatga tttctctgga cgtggtgttg tcgaaactct 12000 caaagtacgc agtcaggaac gtgcgcgaca tgtcgtcggg aaactcgcgc ggaaacatgt 12060 tgttgtaacc gaacgggtcc catagcgcca aaaccaaatc tgccagcgtc aatagaatga 12120 gcacgatgcc gacaatggag ctggcttgga tagcgattcg agttaactag gatctttgtg 12180 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc 12240 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt 12300 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt 12360 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct 12420 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt 12480 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc 12540 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa 12600 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt 12660 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc 12720 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat 12780 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact 12840 catcaatgta tcttatcatg tctggatcga tctggccggc cgtttaaacg aattcttgaa 12900 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 12960 ctta 12964 // ID MN658790; SV 1; linear; other DNA; STD; SYN; 9994 BP. XX AC MN658790; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct Syb::GFPn146i gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-9994 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 031a1049e8435648b388777b57f4701d. XX FH Key Location/Qualifiers FH FT source 1..9994 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,9199..9994) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT 5'UTR 7212..7232 FT /note="Syn21" FT CDS 7233..8525 FT /codon_start=1 FT /transl_table=11 FT /product="Syb::GFPn146i" FT /protein_id="QGY73560.1" FT /translation="MADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQT FT QAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLK FT MMIIMGVIGLVVVGIIANKLGLIGGEQPPQYQYPPQYMQPPPPPPQQPAGGQSSLVDGA FT GAGDGAGGSAGAGDHGGVGGGGSGGGGSSMSKGEELFTGVVPILVELDGDVNGHKFSVS FT GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMP FT EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISH FT NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSAL FT SKDPNEKRDHMVLLEFVTAAGITHGMDELYK" FT misc_feature 7233..7775 FT /note="Drosophila synaptobrevin" FT misc_feature 7548..7613 FT /note="trans-membrane segment" FT misc_feature 7809..8525 FT /note="Drosophila codon optimised EGFP N146I" FT misc_feature 8244..8246 FT /note="N146I mutation" FT 3'UTR 8532..9197 FT /note="P10" FT 3'UTR 9201..9897 FT /note="SV40" XX SQ Sequence 9994 BP; 2664 A; 2352 C; 2387 G; 2591 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg caacttaaaa aaaaaaatca aaatggcgga cgctgcacca gctggcgatg 7260 caccacccaa tgccggagcc ccggccggag agggcggcga tggcgagatt gtgggcggac 7320 cacacaatcc gcagcagatc gcggcacaga agcgtctgca gcagacgcag gcgcaggtcg 7380 atgaggtcgt ggacatcatg cgcacgaacg tggaaaaggt gctggagcgc gacagcaagc 7440 tgtcggagct ggacgaccgt gccgatgcct tgcagcaggg tgcctcgcag tttgagcagc 7500 aggcgggcaa gctcaagagg aaattctggc tccagaactt gaagatgatg atcatcatgg 7560 gcgtgattgg cctggttgtc gtgggcatta ttgcaaataa acttggactc ataggcgggg 7620 agcagccgcc acagtaccag tatcccccac agtacatgca gccaccgccg ccgccaccgc 7680 agcagccagc cggaggacag tcatcgctgg tggatggggc aggagcagga gacggagcag 7740 gaggatcggc aggagctggc gatcacggcg gcgtgggtgg cggcggaagt ggaggtggag 7800 gctcgtccat gtccaaaggt gaagaactgt ttaccggagt agtcccgata ttggttgaac 7860 tcgacggcga tgtcaacggt cataaattca gtgtgtccgg cgagggtgag ggcgacgcca 7920 catacggtaa gctgacgttg aagttcatat gcaccacggg caagctgccc gtgccatggc 7980 cgacgttggt cacgacgctg acgtatggtg tccagtgttt cagccgttac cccgatcata 8040 tgaagcagca tgactttttc aagtcggcga tgccggaggg atacgttcaa gagaggacca 8100 ttttcttcaa ggatgacggc aactataaga cgcgagcgga ggtgaaattt gaaggcgaca 8160 cactcgttaa ccgtattgag ttgaagggca ttgattttaa ggaggatggc aacattctgg 8220 gccataagtt ggaatacaac tacatctcgc ataatgtgta tataatggca gataagcaga 8280 aaaacggaat aaaggttaac ttcaagattc gccacaacat agaggacggt tccgtgcaac 8340 ttgcagatca ttaccaacag aacacaccca ttggagatgg cccagttctc ttgccagaca 8400 atcactacct ttccacacag tccgcgttga gcaaggaccc caatgaaaag cgggaccaca 8460 tggtgttgct ggagtttgtg accgcagctg gtattacaca cggcatggat gagctctaca 8520 agtaatctag aatgaatcgt ttttaaaata acaaatcaat tgttttataa tattcgtacg 8580 attctttgat tatgtaataa aatgtgatca ttaggaagat tacgaaaaat ataaaaaata 8640 tgagttctgt gtgtataaca aatgctgtaa acgccacaat tgtgtttgtt gcaaataaac 8700 ccatgattat ttgattaaaa ttgttgtttt ctttgttcat agacaatagt gtgttttgcc 8760 taaacgtgta ctgcataaac tccatgcgag tgtatagcga gctagtggct aacgcttgcc 8820 ccaccaaagt agattcgtca aaatcctcaa tttcatcacc ctcctccaag tttaacattt 8880 ggccgtcgga attaacttct aaagatgcca cataatctaa taaatgaaat agagattcaa 8940 acgtggcgtc atcgtccgtt tcgaccattt ccgaaaagaa ctcgggcata aactctatga 9000 tttctctgga cgtggtgttg tcgaaactct caaagtacgc agtcaggaac gtgcgcgaca 9060 tgtcgtcggg aaactcgcgc ggaaacatgt tgttgtaacc gaacgggtcc catagcgcca 9120 aaaccaaatc tgccagcgtc aatagaatga gcacgatgcc gacaatggag ctggcttgga 9180 tagcgattcg agttaactag gatctttgtg aaggaacctt acttctgtgg tgtgacataa 9240 ttggacaaac tacctacaga gatttaaagc tctaaggtaa atataaaatt tttaagtgta 9300 taatgtgtta aactactgat tctaattgtt tgtgtatttt agattccaac ctatggaact 9360 gatgaatggg agcagtggtg gaatgccttt aatgaggaaa acctgttttg ctcagaagaa 9420 atgccatcta gtgatgatga ggctactgct gactctcaac attctactcc tccaaaaaag 9480 aagagaaagg tagaagaccc caaggacttt ccttcagaat tgctaagttt tttgagtcat 9540 gctgtgttta gtaatagaac tcttgcttgc tttgctattt acaccacaaa ggaaaaagct 9600 gcactgctat acaagaaaat tatggaaaaa tatttgatgt atagtgcctt gactagagat 9660 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct 9720 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc 9780 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc 9840 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcga 9900 tctggccggc cgtttaaacg aattcttgaa gacgaaaggg cctcgtgata cgcctatttt 9960 tataggttaa tgtcatgata ataatggttt ctta 9994 // ID MN658791; SV 1; linear; other DNA; STD; SYN; 10789 BP. XX AC MN658791; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct Syb::GFPn146i::TEV gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-10789 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 4addc6da93cf2f7000734b6a56a9127e. XX FH Key Location/Qualifiers FH FT source 1..10789 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,9994..10789) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT 5'UTR 7212..7232 FT /note="Syn21" FT CDS 7233..9320 FT /codon_start=1 FT /transl_table=11 FT /product="Syb::GFPn146i::TEV" FT /protein_id="QGY73561.1" FT /translation="MADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQT FT QAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLK FT MMIIMGVIGLVVVGIIANKLGLIGGEQPPQYQYPPQYMQPPPPPPQQPAGGQSSLVDGA FT GAGDGAGGSAGAGDHGGVGGGGSGGGGSSMSKGEELFTGVVPILVELDGDVNGHKFSVS FT GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMP FT EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISH FT NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSAL FT SKDPNEKRDHMVLLEFVTAAGITHGMDELYKGGGGSGGGGSMESLFKGPRDYNPISSTI FT CHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLI FT DGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSSD FT GIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFTNTNNYFTSVPKNFMELLTNQEA FT QQWVSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLMNELVYSQEQKLISEEDLNE FT M" FT misc_feature 7233..7775 FT /note="Drosophila synaptobrevin" FT misc_feature 7548..7613 FT /note="trans-membrane segment" FT misc_feature 7809..8522 FT /note="Drosophila codon optimised EGFP N146I" FT misc_feature 8244..8246 FT /note="N146I mutation" FT misc_feature 8553..9278 FT /note="Drosophila codon optimised TEV" FT misc_feature 9279..9317 FT /note="1xMyc tag" FT 3'UTR 9327..9992 FT /note="P10" FT 3'UTR 9996..10692 FT /note="SV40" XX SQ Sequence 10789 BP; 2852 A; 2603 C; 2604 G; 2730 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg caacttaaaa aaaaaaatca aaatggcgga cgctgcacca gctggcgatg 7260 caccacccaa tgccggagcc ccggccggag agggcggcga tggcgagatt gtgggcggac 7320 cacacaatcc gcagcagatc gcggcacaga agcgtctgca gcagacgcag gcgcaggtcg 7380 atgaggtcgt ggacatcatg cgcacgaacg tggaaaaggt gctggagcgc gacagcaagc 7440 tgtcggagct ggacgaccgt gccgatgcct tgcagcaggg tgcctcgcag tttgagcagc 7500 aggcgggcaa gctcaagagg aaattctggc tccagaactt gaagatgatg atcatcatgg 7560 gcgtgattgg cctggttgtc gtgggcatta ttgcaaataa acttggactc ataggcgggg 7620 agcagccgcc acagtaccag tatcccccac agtacatgca gccaccgccg ccgccaccgc 7680 agcagccagc cggaggacag tcatcgctgg tggatggggc aggagcagga gacggagcag 7740 gaggatcggc aggagctggc gatcacggcg gcgtgggtgg cggcggaagt ggaggtggag 7800 gctcgtccat gtccaaaggt gaagaactgt ttaccggagt agtcccgata ttggttgaac 7860 tcgacggcga tgtcaacggt cataaattca gtgtgtccgg cgagggtgag ggcgacgcca 7920 catacggtaa gctgacgttg aagttcatat gcaccacggg caagctgccc gtgccatggc 7980 cgacgttggt cacgacgctg acgtatggtg tccagtgttt cagccgttac cccgatcata 8040 tgaagcagca tgactttttc aagtcggcga tgccggaggg atacgttcaa gagaggacca 8100 ttttcttcaa ggatgacggc aactataaga cgcgagcgga ggtgaaattt gaaggcgaca 8160 cactcgttaa ccgtattgag ttgaagggca ttgattttaa ggaggatggc aacattctgg 8220 gccataagtt ggaatacaac tacatctcgc ataatgtgta tataatggca gataagcaga 8280 aaaacggaat aaaggttaac ttcaagattc gccacaacat agaggacggt tccgtgcaac 8340 ttgcagatca ttaccaacag aacacaccca ttggagatgg cccagttctc ttgccagaca 8400 atcactacct ttccacacag tccgcgttga gcaaggaccc caatgaaaag cgggaccaca 8460 tggtgttgct ggagtttgtg accgcagctg gtattacaca cggcatggat gagctctaca 8520 agggtggcgg cggaagtgga ggtggaggct cgatggagag cctgttcaag ggcccacgcg 8580 attacaaccc catcagcagc accatctgcc acctgaccaa cgagtccgat ggccacacca 8640 ccagcctgta cggcattggc ttcggcccct tcatcatcac caacaagcac ctgttccgcc 8700 gcaacaacgg caccctgctg gtgcagagtc tgcatggcgt gttcaaagtg aagaatacca 8760 ccaccctgca gcagcatctg atcgatggcc gcgatatgat catcatccgc atgcccaagg 8820 atttcccgcc cttcccgcag aagctgaagt tccgcgagcc acagcgcgag gagcgcattt 8880 gcctcgtgac caccaacttc cagaccaaga gcatgagcag catggtgtcc gataccagct 8940 gcaccttccc cagctccgat ggcatcttct ggaagcactg gattcagacc aaggatggcc 9000 agtgcggcag ccccctggtg tccacccgcg acggattcat cgtgggcatt cacagcgcca 9060 gcaacttcac gaataccaac aactacttca ccagcgtgcc caagaacttc atggagctgc 9120 tgaccaacca ggaggcccag cagtgggtgt ccggatggcg cctgaatgcc gatagcgtgc 9180 tgtggggcgg acacaaggtg ttcatggtca agcccgagga gcccttccag cccgtgaagg 9240 aggccaccca gctgatgaac gagctggtgt actcccagga gcaaaagctc atttctgaag 9300 aggacttgaa tgaaatgtaa tctagaatga atcgttttta aaataacaaa tcaattgttt 9360 tataatattc gtacgattct ttgattatgt aataaaatgt gatcattagg aagattacga 9420 aaaatataaa aaatatgagt tctgtgtgta taacaaatgc tgtaaacgcc acaattgtgt 9480 ttgttgcaaa taaacccatg attatttgat taaaattgtt gttttctttg ttcatagaca 9540 atagtgtgtt ttgcctaaac gtgtactgca taaactccat gcgagtgtat agcgagctag 9600 tggctaacgc ttgccccacc aaagtagatt cgtcaaaatc ctcaatttca tcaccctcct 9660 ccaagtttaa catttggccg tcggaattaa cttctaaaga tgccacataa tctaataaat 9720 gaaatagaga ttcaaacgtg gcgtcatcgt ccgtttcgac catttccgaa aagaactcgg 9780 gcataaactc tatgatttct ctggacgtgg tgttgtcgaa actctcaaag tacgcagtca 9840 ggaacgtgcg cgacatgtcg tcgggaaact cgcgcggaaa catgttgttg taaccgaacg 9900 ggtcccatag cgccaaaacc aaatctgcca gcgtcaatag aatgagcacg atgccgacaa 9960 tggagctggc ttggatagcg attcgagtta actaggatct ttgtgaagga accttacttc 10020 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata 10080 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt 10140 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg 10200 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct 10260 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta 10320 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc 10380 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt gatgtatagt 10440 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 10500 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 10560 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 10620 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 10680 tcatgtctgg atcgatctgg ccggccgttt aaacgaattc ttgaagacga aagggcctcg 10740 tgatacgcct atttttatag gttaatgtca tgataataat ggtttctta 10789 // ID MN658792; SV 1; linear; other DNA; STD; SYN; 10789 BP. XX AC MN658792; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct Syb::GFPn146i::TEVt173v gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-10789 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; d92a5cc29b282c68bad3579e3bc1743a. XX FH Key Location/Qualifiers FH FT source 1..10789 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..7211,9994..10789) FT /note="pJFRC19" FT regulatory 6488..6878 FT /note="13xLexAop2" FT /regulatory_class="enhancer" FT regulatory 6879..7137 FT /note="from Hsp70" FT /regulatory_class="promoter" FT misc_feature 7140..7211 FT /note="IVS" FT 5'UTR 7212..7232 FT /note="Syn21" FT CDS 7233..9320 FT /codon_start=1 FT /transl_table=11 FT /product="Syb::GFPn146i::TEVt173v" FT /protein_id="QGY73562.1" FT /translation="MADAAPAGDAPPNAGAPAGEGGDGEIVGGPHNPQQIAAQKRLQQT FT QAQVDEVVDIMRTNVEKVLERDSKLSELDDRADALQQGASQFEQQAGKLKRKFWLQNLK FT MMIIMGVIGLVVVGIIANKLGLIGGEQPPQYQYPPQYMQPPPPPPQQPAGGQSSLVDGA FT GAGDGAGGSAGAGDHGGVGGGGSGGGGSSMSKGEELFTGVVPILVELDGDVNGHKFSVS FT GEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMP FT EGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISH FT NVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSAL FT SKDPNEKRDHMVLLEFVTAAGITHGMDELYKGGGGSGGGGSMESLFKGPRDYNPISSTI FT CHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLLVQSLHGVFKVKNTTTLQQHLI FT DGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTNFQTKSMSSMVSDTSCTFPSSD FT GIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFVNTNNYFTSVPKNFMELLTNQEA FT QQWVSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQLMNELVYSQEQKLISEEDLNE FT M" FT misc_feature 7233..7775 FT /note="Drosophila synaptobrevin" FT misc_feature 7548..7613 FT /note="trans-membrane segment" FT misc_feature 7809..8522 FT /note="Drosophila codon optimised EGFP N146I" FT misc_feature 8244..8246 FT /note="N146I mutation" FT misc_feature 8553..9278 FT /note="Drosophila codon optimised TEV T173V" FT misc_feature 9069..9071 FT /note="T173V mutation - removes glycosylation site" FT misc_feature 9279..9317 FT /note="1xMyc tag" FT 3'UTR 9327..9992 FT /note="P10" FT 3'UTR 9996..10692 FT /note="SV40" XX SQ Sequence 10789 BP; 2851 A; 2602 C; 2605 G; 2731 T; 0 other; gactcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 60 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 120 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg 180 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa 240 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt 300 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt 360 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat 420 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg 480 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta 540 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat 600 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag 660 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa 720 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca 780 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc 840 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt 900 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc 960 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat 1020 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt 1080 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac 1140 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc 1200 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca 1260 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta 1320 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct 1380 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg 1440 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc 1500 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta 1560 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg 1620 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt 1680 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg 1740 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg 1800 ccttttgctc acatgttacc gtcgacgatg taggtcacgg tctcgaagcc gcggtgcggg 1860 tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat ctggtccatc 1920 atgatgaacg ggtcgaggtg gcggtagttg atcccggcga acgcgcggcg caccgggaag 1980 ccctcgccct cgaaaccgct gggcgcggtg gtcacggtga gcacgggacg tgcgacggcg 2040 tcggcgggtg cggatacgcg gggcagcgtc agcgggttct cgacggtcac ggcgggcatg 2100 tcgacaagcc gaacatatgg gcgcgcctag tatgtatgta agttaataaa acccattttt 2160 gcggaaagta gataaaaaaa acattttttt tttttactgc actggatatc attgaactta 2220 tctgatcagt tttaaattta cttcgatcca agggtatttg atgtaccagg ttctttcgat 2280 tacctctcac tcaaaatgac attccactca aagtcagcgc tgtttgcctc cttctctgtc 2340 cacagaaata tcgccgtctc tttcgccgct gcgtccgcta tctctttcgc caccgtttgt 2400 agcgttacgt agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa 2460 gctccaagcg gtttacgcca tcaattaaac acaaagtgct gtgccaaaac tcctctcgct 2520 tcttattttt gtttgttttt tgagtgattg gggtggtgat tggttttggg tgggtaagca 2580 ggggaaagtg tgaaaaatcc cggcaatggg ccaagaggat caggagctat taattcgcgg 2640 aggcagcaaa cacccatctg ccgagcatct gaacaatgtg agtagtacat gtgcatacat 2700 cttaagttca cttgatctat aggaactgcg attgcaacat caaattgtat gcggcgtgag 2760 aactgcgacc cacaaaaatc ccaaaccgca attgcacaaa caaatagtga cacgaaacag 2820 attattctgg tagctgttct cgctatataa gacaattttt gagatcatat catgatcaag 2880 acatctaaag gcattcattt tcgactatat tcttttttac aaaaaatata acaaccagat 2940 attttaagct gatcctagat gcacaaaaaa taaataaaag tataaaccta cttcgtagga 3000 tacttcgggg tactttttgt tcggggttag atgagcataa cgctagtagt tgatatttga 3060 gatcccctat cattgcaggg tgacagcgga gcggcttcgc agagctgcat taaccagggc 3120 ttcgggcagg ccaaaaacta cggcacgctc cggccaccca gtccgccgga ggactccggt 3180 tcagggagcg gccaactagc cgagaacctc acctatgcct ggcacaatat ggacatcttt 3240 ggggcggtca atcagccggg ctccggatgg cggcagctgg tcaaccggac acgcggacta 3300 ttctgcaacg agcgacacat accggcgccc aggaaacatt tgctcaagaa cggtgagttt 3360 ctattcgcag tcggctgatc tgtgtgaaat cttaataaag ggtccaatta ccaatttgaa 3420 actcagtttg cggcgtggcc tatccgggcg aacttttggc cgtgatgggc agttccggtg 3480 ccggaaagac gaccctgctg aatgcccttg cctttcgatc gccgcagggc atccaagtat 3540 cgccatccgg gatgcgactg ctcaatggcc aacctgtgga cgccaaggag atgcaggcca 3600 ggtgcgccta tgtccagcag gatgacctct ttatcggctc cctaacggcc agggaacacc 3660 tgattttcca agccatggtg cggatgccac gacatctgac ctatcggcag cgagtggccc 3720 gcgtggatca ggtgatccag gagctttcgc tcagcaaatg tcagcacacg atcatcggtg 3780 tgcccggcag ggtgaaaggt ctgtccggcg gagaaaggaa gcgtctggca ttcgcctccg 3840 aggctctaac cgatccgccg cttctgatct gcgatgagcc cacctccgga ctggactcct 3900 ttaccgccca cagcgtcgtc caggtgctga agaagctgtc gcagaagggc aagaccgtca 3960 tcctgaccat tcatcagccg tcttccgagc tgtttgagct ctttgacaag atccttctga 4020 tggccgaggg cagggtagct ttcttgggca ctcccagcga agccgtcgac ttcttttcct 4080 agtgagttcg atgtgtttat taagggtatc tagtattaca taacatctca actcctatcc 4140 agcgtgggtg cccagtgtcc taccaactac aatccggcgg acttttacgt acaggtgttg 4200 gccgttgtgc ccggacggga gatcgagtcc cgtgatcgga tcgccaagat atgcgacaat 4260 tttgccatta gcaaagtagc ccgggatatg gagcagttgt tggccaccaa aaatctggag 4320 aagccactgg agcagccgga gaatgggtac acctacaagg ccacctggtt catgcagttc 4380 cgggcggtcc tgtggcgatc ctggctgtcg gtgctcaagg aaccactcct cgtaaaagtg 4440 cgacttattc agacaacggt gagtggttcc agtggaaaca aatgatataa cgcttacaat 4500 tcttggaaac aaattcgcta gattttagat agaattgcct gattccacac ccttcttagt 4560 ttttttcaat gagatgtata gtttatagtt ttgcagaaga taaataaatt tcatttaact 4620 cgcgaatatt aatgagatgc gagtaacatt ttaatttgca gatggttgcc atcttgattg 4680 gcctcatctt tttgggccaa caactcacgc aagtgggtgt gatgaatatc aacggagcca 4740 tcttcctctt cctgaccaac atgacctttc aaaacgtctt tgccacgata aatgtaagtc 4800 atgtttagaa tacatttgca tttcaataat ttactaactt tctaatgaat cgattcgatt 4860 taggtgttca cctcagagct gccagttttt atgagggagg cccgaagtcg actttatcgc 4920 tgtgacacat actttctggg caaaacgatt gccgaattgc cgctttttct cacagtgcca 4980 ctggtcttca cggcgattgc ctatccgatg atcggactgc gggccggagt gctgcacttc 5040 ttcaactgcc tggcgctggt cactctggtg gccaatgtgt caacgtcctt cggatatcta 5100 atatcctgcg ccagctcctc gacctcgatg gcgctgtctg tgggtccgcc ggttatcata 5160 ccattcctgc tctttggcgg cttcttcttg aactcgggct cggtgccagt atacctcaaa 5220 tggttgtcgt acctctcatg gttccgttac gccaacgagg gtctgctgat taaccaatgg 5280 gcggacgtgg agccgggcga aattagctgc acatcgtcga acaccacgtg ccccagttcg 5340 ggcaaggtca tcctggagac gcttaacttc tccgccgccg atctgccgct ggactacgtg 5400 ggtctggcca ttctcatcgt gagcttccgg gtgctcgcat atctggctct aagacttcgg 5460 gcccgacgca aggagtagcc gacatatatc cgaaataact gcttgttttt tttttttacc 5520 attattacca tcgtgtttac tgtttattgc cccctcaaaa agctaatgta attatatttg 5580 tgccaataaa aacaagatat gacctataga atacaagtat ttccccttcg aacatcccca 5640 caagtagact ttggatttgt cttctaacca aaagacttac acacctgcat accttacatc 5700 aaaaactcgt ttatcgctac ataaaacacc gggatatatt ttttatatac atacttttca 5760 aatcgcgcgc cctcttcata attcacctcc accacaccac gtttcgtagt tgctctttcg 5820 ctgtctccca cccgctctcc gcaacacatt caccttttgt tcgacgacct tggagcgact 5880 gtcgttagtt ccgcgcgatt cggttcgctc aaatggttcc gagtggttca tttcgtctca 5940 atagaaatta gtaataaata tttgtatgta caatttattt gctccaatat atttgtatat 6000 atttccctca cagctatatt tattctaatt taatattatg actttttaag gtaatttttt 6060 gtgacctgtt cggagtgatt agcgttacaa tttgaactga aagtgacatc cagtgtttgt 6120 tccttgtgta gatgcatctc aaaaaaatgg tgggcataat agtgttgttt atatatatca 6180 aaaataacaa ctataataat aagaatacat ttaatttaga aaatgcttgg atttcactgg 6240 aactagggcg cgcctccgga acataatggt gcagggcgct gacttccgcg tttccagact 6300 ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca 6360 gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca 6420 accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca 6480 gggccgcaag cttgcatgcc tgcaggttac tgtacatcca tacagtaagt actgtacatc 6540 catacagtaa gtactgtaca tccatacagt aagtactgta catccataca gtaagtactg 6600 tacatccata cagtaagcgg agactctagc cctagggcat gcctgcaggt tactgtacat 6660 ccatacagta agtactgtac atccatacag taagtactgt acatccatac agtaagcgga 6720 gactctagcg ctagcgcatg cctgcaggtt actgtacatc catacagtaa gtactgtaca 6780 tccatacagt aagtactgta catccataca gtaagtactg tacatccata cagtaagtac 6840 tgtacatcca tacagtaagc ggagactcta gcactagtga cgtcgagcgc cggagtataa 6900 atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa caagcaaagt gaacacgtcg 6960 ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca agctaaacaa tctgcagtaa 7020 agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac caagtaaatc aactgcaact 7080 actgaaatct gccaagaagt aattattgaa tacaagaaga gaactctgaa tagatctaaa 7140 aggtaggttc aaccactgat gcctaggcac accgaaacga ctaaccctaa ttcttatcct 7200 ttacttcagg caacttaaaa aaaaaaatca aaatggcgga cgctgcacca gctggcgatg 7260 caccacccaa tgccggagcc ccggccggag agggcggcga tggcgagatt gtgggcggac 7320 cacacaatcc gcagcagatc gcggcacaga agcgtctgca gcagacgcag gcgcaggtcg 7380 atgaggtcgt ggacatcatg cgcacgaacg tggaaaaggt gctggagcgc gacagcaagc 7440 tgtcggagct ggacgaccgt gccgatgcct tgcagcaggg tgcctcgcag tttgagcagc 7500 aggcgggcaa gctcaagagg aaattctggc tccagaactt gaagatgatg atcatcatgg 7560 gcgtgattgg cctggttgtc gtgggcatta ttgcaaataa acttggactc ataggcgggg 7620 agcagccgcc acagtaccag tatcccccac agtacatgca gccaccgccg ccgccaccgc 7680 agcagccagc cggaggacag tcatcgctgg tggatggggc aggagcagga gacggagcag 7740 gaggatcggc aggagctggc gatcacggcg gcgtgggtgg cggcggaagt ggaggtggag 7800 gctcgtccat gtccaaaggt gaagaactgt ttaccggagt agtcccgata ttggttgaac 7860 tcgacggcga tgtcaacggt cataaattca gtgtgtccgg cgagggtgag ggcgacgcca 7920 catacggtaa gctgacgttg aagttcatat gcaccacggg caagctgccc gtgccatggc 7980 cgacgttggt cacgacgctg acgtatggtg tccagtgttt cagccgttac cccgatcata 8040 tgaagcagca tgactttttc aagtcggcga tgccggaggg atacgttcaa gagaggacca 8100 ttttcttcaa ggatgacggc aactataaga cgcgagcgga ggtgaaattt gaaggcgaca 8160 cactcgttaa ccgtattgag ttgaagggca ttgattttaa ggaggatggc aacattctgg 8220 gccataagtt ggaatacaac tacatctcgc ataatgtgta tataatggca gataagcaga 8280 aaaacggaat aaaggttaac ttcaagattc gccacaacat agaggacggt tccgtgcaac 8340 ttgcagatca ttaccaacag aacacaccca ttggagatgg cccagttctc ttgccagaca 8400 atcactacct ttccacacag tccgcgttga gcaaggaccc caatgaaaag cgggaccaca 8460 tggtgttgct ggagtttgtg accgcagctg gtattacaca cggcatggat gagctctaca 8520 agggtggcgg cggaagtgga ggtggaggct cgatggagag cctgttcaag ggcccacgcg 8580 attacaaccc catcagcagc accatctgcc acctgaccaa cgagtccgat ggccacacca 8640 ccagcctgta cggcattggc ttcggcccct tcatcatcac caacaagcac ctgttccgcc 8700 gcaacaacgg caccctgctg gtgcagagtc tgcatggcgt gttcaaagtg aagaatacca 8760 ccaccctgca gcagcatctg atcgatggcc gcgatatgat catcatccgc atgcccaagg 8820 atttcccgcc cttcccgcag aagctgaagt tccgcgagcc acagcgcgag gagcgcattt 8880 gcctcgtgac caccaacttc cagaccaaga gcatgagcag catggtgtcc gataccagct 8940 gcaccttccc cagctccgat ggcatcttct ggaagcactg gattcagacc aaggatggcc 9000 agtgcggcag ccccctggtg tccacccgcg acggattcat cgtgggcatt cacagcgcca 9060 gcaacttcgt gaataccaac aactacttca ccagcgtgcc caagaacttc atggagctgc 9120 tgaccaacca ggaggcccag cagtgggtgt ccggatggcg cctgaatgcc gatagcgtgc 9180 tgtggggcgg acacaaggtg ttcatggtca agcccgagga gcccttccag cccgtgaagg 9240 aggccaccca gctgatgaac gagctggtgt actcccagga gcaaaagctc atttctgaag 9300 aggacttgaa tgaaatgtaa tctagaatga atcgttttta aaataacaaa tcaattgttt 9360 tataatattc gtacgattct ttgattatgt aataaaatgt gatcattagg aagattacga 9420 aaaatataaa aaatatgagt tctgtgtgta taacaaatgc tgtaaacgcc acaattgtgt 9480 ttgttgcaaa taaacccatg attatttgat taaaattgtt gttttctttg ttcatagaca 9540 atagtgtgtt ttgcctaaac gtgtactgca taaactccat gcgagtgtat agcgagctag 9600 tggctaacgc ttgccccacc aaagtagatt cgtcaaaatc ctcaatttca tcaccctcct 9660 ccaagtttaa catttggccg tcggaattaa cttctaaaga tgccacataa tctaataaat 9720 gaaatagaga ttcaaacgtg gcgtcatcgt ccgtttcgac catttccgaa aagaactcgg 9780 gcataaactc tatgatttct ctggacgtgg tgttgtcgaa actctcaaag tacgcagtca 9840 ggaacgtgcg cgacatgtcg tcgggaaact cgcgcggaaa catgttgttg taaccgaacg 9900 ggtcccatag cgccaaaacc aaatctgcca gcgtcaatag aatgagcacg atgccgacaa 9960 tggagctggc ttggatagcg attcgagtta actaggatct ttgtgaagga accttacttc 10020 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata 10080 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt 10140 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg 10200 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct 10260 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta 10320 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc 10380 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattt gatgtatagt 10440 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa 10500 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa 10560 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 10620 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 10680 tcatgtctgg atcgatctgg ccggccgttt aaacgaattc ttgaagacga aagggcctcg 10740 tgatacgcct atttttatag gttaatgtca tgataataat ggtttctta 10789 // ID MN658793; SV 1; linear; other DNA; STD; SYN; 8134 BP. XX AC MN658793; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct mCherry::TEVs::V5::CD2::GFP gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-8134 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; e504f8b4a462c5a1a52749b011e41bc6. XX FH Key Location/Qualifiers FH FT source 1..8134 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..2674,5373..8134) FT /note="pAWG" FT regulatory 96..2649 FT /note="ActinC5" FT /regulatory_class="promoter" FT CDS 2697..5372 FT /codon_start=1 FT /transl_table=11 FT /product="mCherry::TEVs::V5::CD2::GFP" FT /protein_id="QGY73563.1" FT /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTASHPTFLYKVGMVSKG FT EEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWD FT ILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEF FT IYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEV FT KTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKALSEN FT LYFQSGALSTTLYNKVGKPIPNPLLGLDSTDCRDSGTVWGALGHGINLNIPNFQMTDDI FT DEVRWERGSTLVAEFKRKMKPFLKSGAFEILANGDLKIKNLTRDDSGTYNVTVYSTNGT FT RILDKALDLRILEMVSKPMIYWECSNATLTCEVLEGTDVELKLYQGKEHLRSLRQKTMS FT YQWTNLRAPFKCKAVNRVSQESEMEVVNCPEKGLPLYLIVGVSAGGLLLVFFGALFIFC FT ICKRKKRNRRRKGEELEIKASRMSTVERGPKPHSTQASAPASQNPVASQAPPPPGHHLQ FT TPGHRPLPPSHRNREHQPKKRPPPSGTQVHQQKGPPLPRPRVQPKPPCGSGDVSLPPPN FT YPAFLYKVVSSATMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FT FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG FT NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV FT NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FT FVTAAGITLGMDELYKHRST" FT sig_peptide 2697..2783 FT /note="PAT3 signal peptide" FT misc_feature 2817..3524 FT /note="mCherry" FT misc_feature 3525..3566 FT /note="TEVs" FT misc_feature 3589..3629 FT /note="V5 tag" FT misc_feature 3630..4601 FT /note="CD2 from rat" FT misc_feature 4179..4247 FT /note="transmembrane domain" FT misc_feature 4641..5372 FT /note="EGFP" FT 3'UTR 5373..6095 FT /note="SV40" XX SQ Sequence 8134 BP; 2247 A; 1963 C; 1958 G; 1966 T; 0 other; gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact 60 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat 120 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca 180 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc 240 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat 300 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag 360 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc 420 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc 480 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg 540 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt 600 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc 660 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt 720 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt 780 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat 840 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt 900 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa 960 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg 1020 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg 1080 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc 1140 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc 1200 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt 1260 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact 1320 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc 1380 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat 1440 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg 1500 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca 1560 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt 1620 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag 1680 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct 1740 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt 1800 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca 1860 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc 1920 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct 1980 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta 2040 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa 2100 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat 2160 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac 2220 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac 2280 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga 2340 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt 2400 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg 2460 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc 2520 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc 2580 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa 2640 tccagagacc ccggatccag atatcgacaa gtttgtacaa aaaagcaggc tacaaaatgc 2700 caccttcaac atcattgctg ctcctcgcag cacttcttcc attcgcttta ccagcaagcg 2760 attggaagac tggagaagtc actgctagcc acccaacttt tctatacaaa gttggaatgg 2820 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 2880 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 2940 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 3000 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 3060 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 3120 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg 3180 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 3240 agaagaagac catgggctgg gaggcctcct ccgagagaat gtaccccgag gacggcgccc 3300 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg 3360 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca 3420 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg 3480 ccgagggccg ccactccacc ggcggcatgg acgagctgta caaggccctt agtgagaacc 3540 tgtacttcca gtccggggcc cttagtacaa ctttgtataa taaagttggt aagcctatcc 3600 ctaaccctct cctcggtctc gattctacgg actgcagaga cagtgggacc gtctggggtg 3660 ccctgggtca tggcatcaac ctgaacatcc ctaactttca aatgactgat gatattgatg 3720 aggtgcgatg ggagaggggg agcaccctgg ttgccgagtt taaaaggaag atgaagcctt 3780 ttttgaaatc gggagcattt gagatcttag caaatggaga cttgaagata aagaatctga 3840 caagagatga cagtggcacc tataatgtaa cggtatacag cacaaatggg acacgtatcc 3900 tggacaaggc actggacttg aggatcctag agatggtctc aaagccgatg atctactggg 3960 agtgcagcaa cgcaaccctg acctgtgagg tcttggaagg aacagatgtt gaactaaagc 4020 tgtaccaagg aaaggagcat ctcaggagcc tccgtcagaa gaccatgagt taccagtgga 4080 ccaacctgag agcaccgttt aagtgcaagg cggtaaacag ggtcagccag gagtctgaga 4140 tggaagttgt caactgtcca gagaaaggtc tgcccctcta tctcatagtg ggggtcagtg 4200 caggaggact cctcttggtg ttctttgggg cgctgtttat tttctgtatc tgcaagagga 4260 aaaaacggaa caggaggaga aaaggtgaag agctggagat aaaagcttcc agaatgagca 4320 ctgtggaaag gggacccaag ccacactcaa cccaggcctc agcaccagca tcacagaatc 4380 cagtggcttc ccaagctcct cctccacctg gccatcatct ccagacacct ggccatcgtc 4440 ccctgcctcc aagccaccgt aaccgtgagc accagccgaa gaagagacct cctccatcag 4500 gcacacaggt tcaccagcag aaaggccctc ctttgcccag accccgagtt cagccaaagc 4560 ctccctgtgg gagtggagat gtttcgttgc caccccctaa ttacccagct ttcttgtaca 4620 aagtggtgag ctccgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc 4680 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg 4740 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc 4800 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc 4860 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg 4920 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga 4980 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg 5040 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca 5100 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg 5160 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg 5220 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg 5280 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca 5340 tggacgagct gtacaagcac cggtccacgt gacgtaagct agcaggatct ttgtgaagga 5400 accttacttc tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa 5460 ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt 5520 attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga 5580 ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc 5640 tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc 5700 agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc 5760 tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc 5820 tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc 5880 acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt 5940 tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca 6000 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc 6060 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt 6120 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 6180 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcccgt 6240 ttaaactacg cgtaattcaa acagggttct ggcgtcgttc tcgtactgtt ttccccaggc 6300 cagtgcttta gcgttattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc 6360 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga 6420 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca 6480 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt 6540 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg 6600 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc 6660 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata 6720 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt 6780 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag 6840 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca 6900 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg 6960 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg 7020 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag 7080 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg 7140 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag 7200 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga 7260 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt 7320 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 7380 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 7440 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 7500 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 7560 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 7620 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 7680 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 7740 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 7800 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 7860 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 7920 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 7980 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 8040 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 8100 agcgcagcga gtcagtgagc gaggaagcgg aaga 8134 // ID MN658794; SV 1; linear; other DNA; STD; SYN; 8092 BP. XX AC MN658794; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct mCherry::V5::CD2::GFP gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-8092 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; 507d77a289bab62941e910ee8e55b46e. XX FH Key Location/Qualifiers FH FT source 1..8092 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..2674,5331..8092) FT /note="pAWG" FT regulatory 96..2649 FT /note="ActinC5" FT /regulatory_class="promoter" FT CDS 2697..5330 FT /codon_start=1 FT /transl_table=11 FT /product="mCherry::V5::CD2::GFP" FT /protein_id="QGY73564.1" FT /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTASHPTFLYKVGMVSKG FT EEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWD FT ILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEF FT IYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEV FT KTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYKTTLYN FT KVGKPIPNPLLGLDSTDCRDSGTVWGALGHGINLNIPNFQMTDDIDEVRWERGSTLVAE FT FKRKMKPFLKSGAFEILANGDLKIKNLTRDDSGTYNVTVYSTNGTRILDKALDLRILEM FT VSKPMIYWECSNATLTCEVLEGTDVELKLYQGKEHLRSLRQKTMSYQWTNLRAPFKCKA FT VNRVSQESEMEVVNCPEKGLPLYLIVGVSAGGLLLVFFGALFIFCICKRKKRNRRRKGE FT ELEIKASRMSTVERGPKPHSTQASAPASQNPVASQAPPPPGHHLQTPGHRPLPPSHRNR FT EHQPKKRPPPSGTQVHQQKGPPLPRPRVQPKPPCGSGDVSLPPPNYPAFLYKVVSSATM FT VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPT FT LVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDT FT LVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQ FT LADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDEL FT YKHRST" FT sig_peptide 2697..2783 FT /note="from PAT3" FT misc_feature 2817..3524 FT /note="mCherry" FT misc_feature 3547..3587 FT /note="V5 tag" FT misc_feature 3588..4559 FT /note="CD2 from rat" FT misc_feature 4137..4205 FT /note="transmembrane domain" FT misc_feature 4599..5330 FT /note="EGFP" FT 3'UTR 5331..6053 FT /note="SV40" XX SQ Sequence 8092 BP; 2240 A; 1950 C; 1947 G; 1955 T; 0 other; gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact 60 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat 120 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca 180 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc 240 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat 300 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag 360 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc 420 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc 480 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg 540 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt 600 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc 660 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt 720 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt 780 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat 840 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt 900 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa 960 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg 1020 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg 1080 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc 1140 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc 1200 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt 1260 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact 1320 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc 1380 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat 1440 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg 1500 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca 1560 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt 1620 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag 1680 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct 1740 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt 1800 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca 1860 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc 1920 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct 1980 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta 2040 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa 2100 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat 2160 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac 2220 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac 2280 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga 2340 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt 2400 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg 2460 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc 2520 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc 2580 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa 2640 tccagagacc ccggatccag atatcgacaa gtttgtacaa aaaagcaggc tacaaaatgc 2700 caccttcaac atcattgctg ctcctcgcag cacttcttcc attcgcttta ccagcaagcg 2760 attggaagac tggagaagtc actgctagcc acccaacttt tctatacaaa gttggaatgg 2820 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc 2880 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc 2940 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg 3000 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg 3060 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga 3120 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg 3180 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc 3240 agaagaagac catgggctgg gaggcctcct ccgagagaat gtaccccgag gacggcgccc 3300 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg 3360 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca 3420 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg 3480 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagacaact ttgtataata 3540 aagttggtaa gcctatccct aaccctctcc tcggtctcga ttctacggac tgcagagaca 3600 gtgggaccgt ctggggtgcc ctgggtcatg gcatcaacct gaacatccct aactttcaaa 3660 tgactgatga tattgatgag gtgcgatggg agagggggag caccctggtt gccgagttta 3720 aaaggaagat gaagcctttt ttgaaatcgg gagcatttga gatcttagca aatggagact 3780 tgaagataaa gaatctgaca agagatgaca gtggcaccta taatgtaacg gtatacagca 3840 caaatgggac acgtatcctg gacaaggcac tggacttgag gatcctagag atggtctcaa 3900 agccgatgat ctactgggag tgcagcaacg caaccctgac ctgtgaggtc ttggaaggaa 3960 cagatgttga actaaagctg taccaaggaa aggagcatct caggagcctc cgtcagaaga 4020 ccatgagtta ccagtggacc aacctgagag caccgtttaa gtgcaaggcg gtaaacaggg 4080 tcagccagga gtctgagatg gaagttgtca actgtccaga gaaaggtctg cccctctatc 4140 tcatagtggg ggtcagtgca ggaggactcc tcttggtgtt ctttggggcg ctgtttattt 4200 tctgtatctg caagaggaaa aaacggaaca ggaggagaaa aggtgaagag ctggagataa 4260 aagcttccag aatgagcact gtggaaaggg gacccaagcc acactcaacc caggcctcag 4320 caccagcatc acagaatcca gtggcttccc aagctcctcc tccacctggc catcatctcc 4380 agacacctgg ccatcgtccc ctgcctccaa gccaccgtaa ccgtgagcac cagccgaaga 4440 agagacctcc tccatcaggc acacaggttc accagcagaa aggccctcct ttgcccagac 4500 cccgagttca gccaaagcct ccctgtggga gtggagatgt ttcgttgcca ccccctaatt 4560 acccagcttt cttgtacaaa gtggtgagct ccgccaccat ggtgagcaag ggcgaggagc 4620 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 4680 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 4740 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 4800 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 4860 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 4920 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 4980 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 5040 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 5100 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 5160 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 5220 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 5280 ccgggatcac tctcggcatg gacgagctgt acaagcaccg gtccacgtga cgtaagctag 5340 caggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca aactacctac 5400 agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg ttaaactact 5460 gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat gggagcagtg 5520 gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga 5580 tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa aggtagaaga 5640 ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt ttagtaatag 5700 aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc tatacaagaa 5760 aattatggaa aaatattctg taacctttat aagtaggcat aacagttata atcataacat 5820 actgtttttt cttactccac acaggcatag agtgtctgct attaataact atgctcaaaa 5880 attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt tgatgtatag 5940 tgccttgact agagatcata atcagccata ccacatttgt agaggtttta cttgctttaa 6000 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta 6060 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 6120 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 6180 atcatgtctg gatcccgttt aaactacgcg taattcaaac agggttctgg cgtcgttctc 6240 gtactgtttt ccccaggcca gtgctttagc gttattgaaa aaggaagagt atgagtattc 6300 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 6360 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 6420 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 6480 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 6540 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 6600 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 6660 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 6720 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 6780 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 6840 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 6900 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 6960 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 7020 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 7080 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 7140 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 7200 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 7260 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 7320 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 7380 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 7440 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact 7500 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 7560 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 7620 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 7680 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 7740 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 7800 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 7860 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 7920 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 7980 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 8040 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa ga 8092 // ID MN658795; SV 1; linear; other DNA; STD; SYN; 7498 BP. XX AC MN658795; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct TEV::CD2 gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-7498 RA Cachero S., Jefferis G.S.X.E.; RT ; RL Submitted (08-NOV-2019) to the INSDC. RL Division of Neurobiology, MRC Laboratory of Molecular Biology, Francis RL Crick Avenue, Cambridge, Cambridgeshire CB2 OQH, United Kingdom XX DR MD5; b830329f42c34f29e4d95c27a06039bd. XX FH Key Location/Qualifiers FH FT source 1..7498 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT misc_feature join(1..2674,4559..7498) FT /note="pAWH" FT regulatory 96..2649 FT /note="ActinC5" FT /regulatory_class="promoter" FT CDS 2697..4547 FT /codon_start=1 FT /transl_table=11 FT /product="TEV::CD2" FT /protein_id="QGY73565.1" FT /translation="MPPSTSLLLLAALLPFALPASDWKTGEVTASHPTFLYKVGGHHHH FT HHHGESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKHLFRRNNGTLL FT VQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQREERICLVTTN FT FQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIVGIHSASNFTN FT TNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEEPFQPVKEATQ FT LMNTTLYNKVGDCRDSGTVWGALGHGINLNIPNFQMTDDIDEVRWERGSTLVAEFKRKM FT KPFLKSGAFEILANGDLKIKNLTRDDSGTYNVTVYSTNGTRILDKALDLRILEMVSKPM FT IYWECSNATLTCEVLEGTDVELKLYQGKEHLRSLRQKTMSYQWTNLRAPFKCKAVNRVS FT QESEMEVVNCPEKGLPLYLIVGVSAGGLLLVFFGALFIFCICKRKKRNRRRKGEELEIK FT ASRMSTVERGPKPHSTQASAPASQNPVASQAPPPPGHHLQTPGHRPLPPSHRNREHQPK FT KRPPPSGTQVHQQKGPPLPRPRVQPKPPCGSGDVSLPPPN" FT misc_feature 2697..3548 FT /note="E. coli codon usage TEV" FT sig_peptide 2697..2783 FT /note="PAT3 signal peptide" FT misc_feature 2820..2840 FT /note="polyhistidine" FT misc_feature 3573..4544 FT /note="CD2 from rat" FT misc_feature 4122..4190 FT /note="transmembrane domain" XX SQ Sequence 7498 BP; 2149 A; 1661 C; 1717 G; 1971 T; 0 other; gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact 60 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat 120 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca 180 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc 240 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat 300 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag 360 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc 420 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc 480 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg 540 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt 600 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc 660 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt 720 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt 780 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat 840 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt 900 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa 960 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg 1020 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg 1080 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc 1140 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc 1200 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt 1260 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact 1320 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc 1380 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat 1440 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg 1500 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca 1560 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt 1620 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag 1680 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct 1740 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt 1800 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca 1860 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc 1920 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct 1980 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta 2040 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa 2100 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat 2160 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac 2220 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac 2280 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga 2340 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt 2400 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg 2460 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc 2520 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc 2580 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa 2640 tccagagacc ccggatccag atatcgacaa gtttgtacaa aaaagcaggc tacaaaatgc 2700 caccttcaac atcattgctg ctcctcgcag cacttcttcc attcgcttta ccagcaagcg 2760 attggaagac tggagaagtc actgctagcc acccaacttt tctatacaaa gttggaggtc 2820 atcatcatca tcatcatcat ggagaaagct tgtttaaggg gccgcgtgat tacaacccga 2880 tatcgagcac catttgtcat ttgacgaatg aatctgatgg gcacacaaca tcgttgtatg 2940 gtattggatt tggtcccttc atcattacaa acaagcactt gtttagaaga aataatggaa 3000 cactgttggt ccaatcacta catggtgtat tcaaggtcaa gaacaccacg actttgcaac 3060 aacacctcat tgatgggagg gacatgataa ttattcgcat gcctaaggat ttcccaccat 3120 ttcctcaaaa gctgaaattt agagagccac aaagggaaga gcgcatatgt cttgtgacaa 3180 ccaacttcca aactaagagc atgtctagca tggtgtcaga cactagttgc acattccctt 3240 catctgatgg catattctgg aagcattgga ttcaaaccaa ggatgggcag tgtggcagtc 3300 cattagtatc aactagagat gggttcattg ttggtataca ctcagcatcg aatttcacca 3360 acacaaacaa ttatttcaca agcgtgccga aaaacttcat ggaattgttg acaaatcagg 3420 aggcgcagca gtgggttagt ggttggcgat taaatgctga ctcagtattg tgggggggcc 3480 ataaagtttt catggtgaaa cctgaagagc cttttcagcc agttaaggaa gcgactcaac 3540 tcatgaatac aactttgtat aataaagttg gagactgcag agacagtggg accgtctggg 3600 gtgccctggg tcatggcatc aacctgaaca tccctaactt tcaaatgact gatgatattg 3660 atgaggtgcg atgggagagg gggagcaccc tggttgccga gtttaaaagg aagatgaagc 3720 cttttttgaa atcgggagca tttgagatct tagcaaatgg agacttgaag ataaagaatc 3780 tgacaagaga tgacagtggc acctataatg taacggtata cagcacaaat gggacacgta 3840 tcctggacaa ggcactggac ttgaggatcc tagagatggt ctcaaagccg atgatctact 3900 gggagtgcag caacgcaacc ctgacctgtg aggtcttgga aggaacagat gttgaactaa 3960 agctgtacca aggaaaggag catctcagga gcctccgtca gaagaccatg agttaccagt 4020 ggaccaacct gagagcaccg tttaagtgca aggcggtaaa cagggtcagc caggagtctg 4080 agatggaagt tgtcaactgt ccagagaaag gtctgcccct ctatctcata gtgggggtca 4140 gtgcaggagg actcctcttg gtgttctttg gggcgctgtt tattttctgt atctgcaaga 4200 ggaaaaaacg gaacaggagg agaaaaggtg aagagctgga gataaaagct tccagaatga 4260 gcactgtgga aaggggaccc aagccacact caacccaggc ctcagcacca gcatcacaga 4320 atccagtggc ttcccaagct cctcctccac ctggccatca tctccagaca cctggccatc 4380 gtcccctgcc tccaagccac cgtaaccgtg agcaccagcc gaagaagaga cctcctccat 4440 caggcacaca ggttcaccag cagaaaggcc ctcctttgcc cagaccccga gttcagccaa 4500 agcctccctg tgggagtgga gatgtttcgt tgccaccccc taattaatac ccagctttct 4560 tgtacaaagt ggtgagctcc gccaccatgg atctccaccg cggtggaggc cgcatctttt 4620 acccatacga tgttcctgac tatgcgggct atccctatga cgtcccggac tatgcaggat 4680 cctatccata tgacgttcca gattacgctg ctcatggcgg acaccggtcc acgtgacgta 4740 agctagcagg atctttgtga aggaacctta cttctgtggt gtgacataat tggacaaact 4800 acctacagag atttaaagct ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa 4860 actactgatt ctaattgttt gtgtatttta gattccaacc tatggaactg atgaatggga 4920 gcagtggtgg aatgccttta atgaggaaaa cctgttttgc tcagaagaaa tgccatctag 4980 tgatgatgag gctactgctg actctcaaca ttctactcct ccaaaaaaga agagaaaggt 5040 agaagacccc aaggactttc cttcagaatt gctaagtttt ttgagtcatg ctgtgtttag 5100 taatagaact cttgcttgct ttgctattta caccacaaag gaaaaagctg cactgctata 5160 caagaaaatt atggaaaaat attctgtaac ctttataagt aggcataaca gttataatca 5220 taacatactg ttttttctta ctccacacag gcatagagtg tctgctatta ataactatgc 5280 tcaaaaattg tgtaccttta gctttttaat ttgtaaaggg gttaataagg aatatttgat 5340 gtatagtgcc ttgactagag atcataatca gccataccac atttgtagag gttttacttg 5400 ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat gcaattgttg 5460 ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt 5520 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg 5580 tatcttatca tgtctggatc ccgtttaaac tacgcgtaat tcaaacaggg ttctggcgtc 5640 gttctcgtac tgttttcccc aggccagtgc tttagcgtta ttgaaaaagg aagagtatga 5700 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt 5760 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag 5820 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag 5880 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta 5940 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg 6000 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca 6060 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag 6120 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc 6180 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg 6240 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc 6300 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg 6360 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg 6420 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga 6480 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac 6540 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa 6600 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 6660 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 6720 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 6780 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 6840 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 6900 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 6960 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 7020 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 7080 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 7140 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 7200 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 7260 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 7320 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct 7380 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 7440 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaga 7498 // ID MT346027; SV 1; circular; other DNA; STD; SYN; 2477 BP. XX AC MT346027; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning vector pY71-sfGFP, complete sequence. XX KW . XX OS Cloning vector pY71-sfGFP OC other sequences; artificial sequences; vectors. XX RN [1] RC Publication Status: Online-Only RP 1-2477 RX PUBMED; 32637668. RA Lee M.S., Hung C.S., Phillips D.A., Buck C.C., Gupta M.K., Lux M.W.; RT "Silk fibroin as an additive for cell-free protein synthesis"; RL Synth Syst Biotechnol 5(3):145-154(2020). XX RN [2] RP 1-2477 RA Lee M.S.; RT ; RL Submitted (16-APR-2020) to the INSDC. RL US Army CCDC Chemical Biological Center, 8567 Ricketts Point Road, RL Gunpowder, MD 21010, USA XX DR MD5; aeb26d4442ecb05220a8ed8f9e9fac62. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..2477 FT /organism="Cloning vector pY71-sfGFP" FT /mol_type="other DNA" FT /db_xref="taxon:2754840" FT regulatory 12..601 FT /note="ColE1 origin of replication" FT /regulatory_class="replication_regulatory_region" FT regulatory 628..649 FT /note="T7 promoter R0085" FT /regulatory_class="promoter" FT gene 708..1424 FT /gene="sfGFP" FT CDS 708..1424 FT /codon_start=1 FT /transl_table=11 FT /gene="sfGFP" FT /product="sfGFP" FT /note="super folder green fluorescent protein" FT /protein_id="QLI61463.1" FT /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATIGKLTLK FT FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG FT KYKTRAVVKFEGDTLVNRIELKGTDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA FT NFTVRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQTVLSKDPNEKRDHMVLHE FT YVNAAGITWSHPQFEK" FT gene complement(1558..2373) FT /gene="agp" FT CDS complement(1558..2373) FT /codon_start=1 FT /transl_table=11 FT /gene="agp" FT /product="aminoglucoside phosphotransferase" FT /note="kanamycin resistance protein; KanR" FT /protein_id="QLI61464.1" FT /translation="MSHIQRETSCSKPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" XX SQ Sequence 2477 BP; 695 A; 571 C; 596 G; 615 T; 0 other; ggatcctgca gttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 60 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 120 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 180 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 240 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 300 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 360 gagcgaacga cctacaccga actgagatac ctacagcgtg agcattgaga aagcgccacg 420 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 480 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 540 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 600 cgaattcaga tctcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt 660 ttccctctag aaataatttt gtttaacttt aagaaggaga tatacatatg agcaaaggtg 720 aagaactgtt taccggcgtt gtgccgattc tggtggaact ggatggcgat gtgaacggtc 780 acaaattcag cgtgcgtggt gaaggtgaag gcgatgccac gattggcaaa ctgacgctga 840 aatttatctg caccaccggc aaactgccgg tgccgtggcc gacgctggtg accaccctga 900 cctatggcgt tcagtgtttt agtcgctatc cggatcacat gaaacgtcac gatttcttta 960 aatctgcaat gccggaaggc tatgtgcagg aacgtacgat tagctttaaa gatgatggca 1020 aatataaaac gcgcgccgtt gtgaaatttg aaggcgatac cctggtgaac cgcattgaac 1080 tgaaaggcac ggattttaaa gaagatggca atatcctggg ccataaactg gaatacaact 1140 ttaatagcca taatgtttat attacggcgg ataaacagaa aaatggcatc aaagcgaatt 1200 ttaccgttcg ccataacgtt gaagatggca gtgtgcagct ggcagatcat tatcagcaga 1260 ataccccgat tggtgatggt ccggtgctgc tgccggataa tcattatctg agcacgcaga 1320 ccgttctgtc taaagatccg aacgaaaaac gggaccacat ggttctgcac gaatatgtga 1380 atgcggcagg tattacgtgg agccatccgc agttcgaaaa ataataagtc gaccggctgc 1440 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata 1500 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaagcca attctgatta 1560 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc 1620 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag 1680 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat 1740 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga 1800 atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc 1860 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc 1920 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg 1980 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc 2040 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc 2100 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag 2160 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa 2220 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt 2280 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggctt 2340 cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta 2400 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag 2460 attttgagac acaacgt 2477 // ID MT346028; SV 1; circular; other DNA; STD; SYN; 5295 BP. XX AC MT346028; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning vector pJV298-Tyr1, complete sequence. XX KW . XX OS Cloning vector pJV298-Tyr1 OC other sequences; artificial sequences; vectors. XX RN [1] RC Publication Status: Online-Only RP 1-5295 RX PUBMED; 32637668. RA Lee M.S., Hung C.S., Phillips D.A., Buck C.C., Gupta M.K., Lux M.W.; RT "Silk fibroin as an additive for cell-free protein synthesis"; RL Synth Syst Biotechnol 5(3):145-154(2020). XX RN [2] RP 1-5295 RA Lee M.S.; RT ; RL Submitted (16-APR-2020) to the INSDC. RL US Army CCDC Chemical Biological Center, 8567 Ricketts Point Road, RL Gunpowder, MD 21010, USA XX DR MD5; 8080ec8697cd02bf46104cfbab56492b. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..5295 FT /organism="Cloning vector pJV298-Tyr1" FT /mol_type="other DNA" FT /db_xref="taxon:2754841" FT regulatory 1..546 FT /note="p15a origin of replication" FT /regulatory_class="replication_regulatory_region" FT gene complement(767..1726) FT /gene="lacI" FT CDS complement(767..1726) FT /codon_start=1 FT /transl_table=11 FT /gene="lacI" FT /product="LacI" FT /note="Lac repressor" FT /protein_id="QLI61465.1" FT /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR FT ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA FT LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY FT LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR FT VGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVS FT LVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ" FT regulatory complement(1850..1927) FT /note="LacIq promoter" FT /regulatory_class="promoter" FT regulatory 2161..2189 FT /note="tac promoter" FT /regulatory_class="promoter" FT regulatory 2193..2215 FT /note="LacO operator" FT /regulatory_class="other" FT gene 2277..3170 FT /gene="tyr1" FT CDS 2277..3170 FT /codon_start=1 FT /transl_table=11 FT /gene="tyr1" FT /product="tyrosinase" FT /note="Tyr1 tyrosinase; derived from Bacillus megaterium" FT /protein_id="QLI61466.1" FT /translation="MGNKYRVRKNVLHLTDTEKRDFVRTVLILKEKGIYDRYIAWHGAA FT GKFHTPPGSDRNAAHMSSAFLPWHREYLLRFERDLQSINPEVTLPYWEWETDAQMQDPS FT QSQIWSADFMGGNGNPIKDFIVDTGPFAAGRWTTIDEQGNPSGGLKRNFGATKEAPTLP FT TRDDVLNALKITQYDTPPWDMTSQNSFRNQLEGFINGPQLHNRVHRWVGGQMGVVPTAP FT NDPVFFLHHANVDRIWAVWQIIHRNQNYQPMKNGPFGQNFRDPMYPWNTTPEDVMNHRK FT LGYVYDIELRKSKRSS" FT gene complement(4103..4741) FT /gene="cat" FT CDS complement(4103..4741) FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="Cat" FT /note="chloramphenicol acetyltransferase" FT /protein_id="QLI61467.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRCLMNTTVLR" FT regulatory complement(4724..4844) FT /note="cat promoter" FT /regulatory_class="promoter" XX SQ Sequence 5295 BP; 1345 A; 1315 C; 1304 G; 1331 T; 0 other; tttccatagg ctccgccccc ctgacaagca tcacgaaatc tgacgctcaa atcagtggtg 60 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggcggct ccctcgtgcg 120 ctctcctgtt cctgcctttc ggtttaccgg tgtcattccg ctgttatggc cgcgtttgtc 180 tcattccacg cctgacactc agttccgggt aggcagttcg ctccaagctg gactgtatgc 240 acgaaccccc cgttcagtcc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 300 acccggaaag acatgcaaaa gcaccactgg cagcagccac tggtaattga tttagaggag 360 ttagtcttga agtcatgcgc cggttaaggc taaactgaaa ggacaagttt tggtgactgc 420 gctcctccaa gccagttacc tcggttcaaa gagttggtag ctcagagaac cttcgaaaaa 480 ccgccctgca aggcggtttt ttcgttttca gagcaagaga ttacgcgcag accaaaacga 540 tctcaagaag atcatcttat taatcagata atatattttt agcggagtga ctataaaaag 600 gcgcactgat caatgcgcct tttttataag atcataaaag acccttcatt tatgaagggt 660 ctttttgcat gcggcgcctg atctgtcgag ccgaacgcca gcaagacgta gcccagcgcg 720 tcggccagct tgcaattcgc gctaacttac attaattgcg ttgcgctcac tgcccgcttt 780 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 840 cggtttgcgt attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct 900 gattgccctt caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc 960 ccagcaggcg aaaatcctgt ttgatggtgg ttaacggcgg gatataacat gagctgtctt 1020 cggtatcgtc gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa 1080 tggcgcgcat tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga 1140 tgccctcatt cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt 1200 cccgttccgc tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac 1260 gcagacgcgc cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca 1320 atgcgaccag atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt 1380 tgatgggtgt ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt 1440 ccacagcaat ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt 1500 gcgcgagaag attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg 1560 acaccaccac gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg 1620 acggcgcgtg cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg 1680 ccagttgttg tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt 1740 tttcccgcgt tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat 1800 aagagacacc ggcatactct gcgacatcgt ataacgttac tggtttcaca ttcaccaccc 1860 tgaattgact ctcttccggg cgctatcatg ccataccgcg aaaggttttg caccattcga 1920 tggtgtcaac gtaaatgcat gccgcttcgc cttcgcgcgc gaattgcaag ctgatccggg 1980 cttatcgact gcacggtgca ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt 2040 atggctgtgc aggtcgtaaa tcactgcata attcgtgtcg ctcaaggcgc actcccgttc 2100 tggataatgt tttttgcgcc gacatcataa cggttctggc aaatattctg aaatgagctg 2160 ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca 2220 caggaaacag cctcgacagg cctaggaatt cggcgtaagc ttaaggagat atacatatgg 2280 gtaacaagta tagagttaga aaaaacgtat tacatcttac cgacacggaa aaaagagatt 2340 ttgttcgtac cgtgctaata ctaaaggaaa aagggatata tgaccgctat atagcctggc 2400 atggtgcagc aggtaaattt catactcctc cgggcagcga tcgaaatgca gcacatatga 2460 gttctgcttt tttaccgtgg catcgtgaat accttttacg attcgaacgt gaccttcagt 2520 caatcaatcc agaagtaacc cttccttatt gggaatggga aacggacgca cagatgcagg 2580 atccctcaca atcacaaatt tggagtgcag attttatggg aggaaacgga aatcccataa 2640 aagattttat cgtcgatacc gggccatttg cagctgggcg ctggacgacg atcgatgaac 2700 aaggaaatcc ttccggaggg ctaaaacgta attttggagc aacgaaagag gcacctacac 2760 tccctactcg agatgatgtc ctcaatgctt taaaaataac tcagtatgat acgccgcctt 2820 gggatatgac cagccaaaac agctttcgta atcagcttga aggatttatt aacgggccac 2880 agcttcacaa tcgcgtacac cgttgggttg gcggacagat gggcgttgtg cctactgctc 2940 cgaatgatcc tgtcttcttt ttacaccacg caaatgtgga tcgtatttgg gctgtatggc 3000 aaattattca tcgtaatcaa aactatcagc cgatgaaaaa cgggccattt ggtcaaaact 3060 ttagagatcc gatgtaccct tggaatacaa cccctgaaga cgttatgaac catcgaaagc 3120 ttgggtacgt atacgatata gaattaagaa aatcaaaacg ttcctcatga ggatccggtg 3180 attgattgag caagctttat gcttgtaaac cgttttgtga aaaaattttt aaaataaaaa 3240 aggggacctc tagggtcccc aattactagc caattcgatt actggcttac tatgttggca 3300 ctgatgaggg tgtcagtgaa gtgacctgca gggggggggg gcgctgaggt ctgcctcgtg 3360 aagaaggtgt tgctgactca taccaggctt aattcctgca tttgcctgtt tccagtcggt 3420 agatattcca caaaacagca gggaagcagc gcttttccgc tgcataaccc tgcttcgggg 3480 tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc 3540 aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg 3600 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg 3660 tgctcaacgg gaatcctgct ctgcgaggct ggccggctac cgccggcgta acagatgagg 3720 gcaagcggat ggctgatgaa accaagccaa ccaggaaggg cagcccacct atcaaggtgt 3780 actgccttcc agaattaagc ctgaatcgcc ccatcatcca gccagaaagt gagggagcca 3840 cggttgatga gagctttgtt gtaggtggac cagttggtga ttttgaactt ttgctttgcc 3900 acggaacggt ctgcgttgtc gggaagatgc gtgatctgat ccttcaactc agcaaaagtt 3960 cgatttattc aacaaagccg ccgtcccgtc aagtcagcgt aatgctctgc cagtgttaca 4020 accaattaac caattctaga gaataggaac ttcggaatag gaacttcatt taaatggcgc 4080 gccttacgcc ccgccctgcc actcatcgca gtactgttgt attcattaag catctgccga 4140 catggaagcc atcacaaacg gcatgatgaa cctgaatcgc cagcggcatc agcaccttgt 4200 cgccttgcgt ataatatttg cccatggtga aaacgggggc gaagaagttg tccatattgg 4260 ccacgtttaa atcaaaactg gtgaaactca cccagggatt ggctgagacg aaaaacatat 4320 tctcaataaa ccctttaggg aaataggcca ggttttcacc gtaacacgcc acatcttgcg 4380 aatatatgtg tagaaactgc cggaaatcgt cgtggtattc actccagagc gatgaaaacg 4440 tttcagtttg ctcatggaaa acggtgtaac aagggtgaac actatcccat atcaccagct 4500 caccgtcttt cattgccata cgtaattccg gatgagcatt catcaggcgg gcaagaatgt 4560 gaataaaggc cggataaaac ttgtgcttat ttttctttac ggtctttaaa aaggccgtaa 4620 tatccagctg aacggtctgg ttataggtac attgagcaac tgactgaaat gcctcaaaat 4680 gttctttacg atgccattgg gatatatcaa cggtggtata tccagtgatt tttttctcca 4740 ttttagcttc cttagctcct gaaaatctcg acaactcaaa aaatacgccc ggtagtgatc 4800 ttatttcatt atggtgaaag ttggaacctc ttacgtgccg atcaacgtct cattttcgcc 4860 aaaagttggc ccagggcttc ccggtatcaa cagggacacc aggatttatt tattctgcga 4920 agtgatcttc cgtcacaggt aggcgcgccg aagttcctat actttctaga attactgttt 4980 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat 5040 cagagatttt gagacacaac gtggctttcc cccccccccc tgcaggtcct tcatgtggca 5100 ggagaaaaaa ggctgcaccg gtgcgtcagc agaatatgtg atacaggata tattccgctt 5160 cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg 5220 aacggggcgg agatttcctg gaagatgcca ggaagatact taacagggaa gtgagagggc 5280 cgcggcaaag ccgtt 5295 // ID MT396223; SV 1; linear; other DNA; STD; SYN; 2211 BP. XX AC MT396223; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct clone AAV3b-DE5 capsid protein VP1 (cap) gene, complete DE cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-2211 RA Biswas M., Marsic D., Li N., Zou C., Zolotukhin I., Kumar S.R.P., RA Butterfield J.S.S., Kondratov O., de Jong Y.P., Herzog R.W., Zolotukhin S.; RT "Engineering and in vitro selection of a novel AAV3B variant with high RT hepatocyte tropism and improved immune evasion"; RL Unpublished. XX RN [2] RP 1-2211 RA Marsic D., Biswas M., Zolotukhin S., Herzog R.; RT ; RL Submitted (25-APR-2020) to the INSDC. RL R&D, Porton Biologics, 388 Xinping Street, Suzhou, Jiangsu 215100, China XX DR MD5; 59425201fa885c93f0bc818d9cb8935c. XX CC ##Assembly-Data-START## CC Assembly Method :: personal python scripts v. 2017 CC Sequencing Technology :: PacBio CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..2211 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /clone="AAV3b-DE5" FT /note="derived from Adeno-associated virus AAV3B by FT directed evolution" FT /db_xref="taxon:32630" FT gene 1..2211 FT /gene="cap" FT CDS 1..2211 FT /codon_start=1 FT /transl_table=11 FT /gene="cap" FT /product="capsid protein VP1" FT /protein_id="QLI60567.1" FT /translation="MAADGYLPDWLEDNLSEGIREWWALKPGVPQPKANQQHQDNRRGL FT VLPGYKYLGPGNGLDKGEPVNEADAAALEHDKAYDQQLKAGDNPYLKYNHADAEFQERL FT QEDTSFGGNLGRAVFQAKKRILEPLGLVEEAAKTAPGKKRPVDQSPQEPDSSSGVGKSG FT KQPARKRLNFGQTGDSESVPDPQPLGEPPAAPTSLGSNTMASGGGAPMADNNEGADGVG FT NSSGNWHCDSQWLGDRVITTSTRTWALPTYNNHLYKQISSASGASNDNHYFGYSTPWGY FT FDFNRFHCHFSPRDWQRLINNNWGFRPKKLSFKLFNIQVKEVTQNDGTTTIANNLTSTV FT QVFTDSEYQLPYVLGSAHQGCLPPFPADVFMVPQYGYLTLNNGSQAVGRSSFYCLEYFP FT SQMLRTGNNFQFSYTFEDVPFHSSYAHSQSLDRLMNPLIDQYLYYLNRTQSTPGGTTGT FT NGLKFSQAGPQSMSLQARNWLPGPCYRQQRLSKIPSQNNNSNFPWTAASKYHLNGRDSL FT VNPGPAMASHKDDDDRFFPMHGNLIFGKQGAGRDNTEYDHVMITDEEEIRTTNPVATEQ FT YGTVANNLQSSNTAPTTRTVNDQGALPGMVWQDRDVYLQGPIWAKIPHTDGHFHPSPLM FT GGFGLKHPPPQIMIKNTPVPANPPTTFSPAKFASFITQYSTGQVSVEIEWELQKENSKR FT WNPEIQYTSNYNKSVNVDFTVDTNGVYSEPRPIGTRYLTRNL" XX SQ Sequence 2211 BP; 606 A; 615 C; 527 G; 463 T; 0 other; atggctgctg acggttatct tccagattgg ctcgaggaca acctttctga aggcattcgt 60 gagtggtggg ctctgaaacc tggagtccct caacccaaag cgaaccaaca acaccaggac 120 aaccgtcggg gtcttgtgct tccgggttac aaatacctcg gacccggtaa cggactcgac 180 aaaggagagc cggtcaacga ggcggacgcg gcagccctcg aacacgacaa agcttacgac 240 cagcagctca aggccggtga caacccgtac ctcaagtaca accacgccga cgccgagttt 300 caggagcgtc ttcaagaaga tacgtctttt gggggcaacc ttggcagagc agtcttccag 360 gccaaaaaga ggatccttga gcctcttggt ctggttgagg aagcagctaa aacggctcct 420 ggaaagaaga ggcctgtaga tcagtctcct caggaaccgg actcatcatc tggtgttggc 480 aaatcgggca aacagcctgc cagaaaaaga ctaaatttcg gtcagactgg cgactcagag 540 tcagtcccag accctcaacc tctcggagaa ccaccagcag cccccacaag tttgggatct 600 aatacaatgg cttcaggcgg tggcgcacca atggcagaca ataacgaggg tgccgatgga 660 gtgggtaatt cctcaggaaa ttggcattgc gattcccaat ggctgggcga cagagtcatc 720 accaccagca ccagaacctg ggccctgccc acttacaaca accatctcta caagcaaatc 780 tccagcgcat caggagctag caacgacaac cactactttg gctacagcac cccttggggg 840 tattttgact ttaacagatt ccactgccac ttctcaccac gtgactggca gcgactcatt 900 aacaacaact ggggattccg gcccaagaaa ctcagcttca agctcttcaa catccaagtt 960 aaagaggtca cgcagaacga tggcacgacg actattgcca ataaccttac cagcacggtt 1020 caagtgttta cggactcgga gtatcagctc ccgtacgtgc tcgggtcggc gcaccaaggc 1080 tgtctcccgc cgtttccagc ggacgtcttc atggtccctc agtatggata cctcaccctg 1140 aacaacggaa gtcaagcggt gggacgctca tccttttact gcctggagta cttcccttcg 1200 cagatgctaa ggactggaaa taacttccaa ttcagctata ccttcgagga tgtacctttt 1260 cacagcagct acgctcacag ccagagtttg gatcgcttga tgaatcctct tattgatcag 1320 tatctgtact acctgaacag aacgcaaagc acccccggcg gaacaaccgg cacgaacggg 1380 ctgaagttta gccaggctgg gcctcagtct atgtctttgc aggccagaaa ttggctacct 1440 gggccctgct accggcaaca gagactttca aaaatcccca gccagaacaa caacagtaac 1500 tttccttgga cagcggccag caaatatcat ctcaatggcc gcgactcgct ggtgaatcca 1560 ggaccagcta tggccagtca caaggacgat gacgacagat ttttccctat gcacggcaat 1620 ctaatatttg gcaaacaagg cgccgggaga gacaataccg agtacgacca cgtaatgatt 1680 acggatgaag aagagattcg taccaccaat cctgtggcaa cagagcagta tggaactgtg 1740 gcaaataact tgcagagctc aaatacagct cccacgacta gaactgtcaa tgatcagggg 1800 gccttacctg gcatggtgtg gcaagatcgt gacgtctacc ttcaaggacc tatctgggca 1860 aagattcctc acacggatgg acactttcat ccttctcctc tgatgggagg ctttggactg 1920 aaacatccgc ctcctcaaat catgatcaaa aatactccgg taccggcaaa tcctccgacg 1980 actttcagcc cggccaagtt tgcttcattt atcactcagt actccactgg acaggtcagc 2040 gtggaaattg agtgggagct acagaaagaa aacagcaaac gttggaatcc agagattcag 2100 tacacttcca actacaacaa gtctgttaat gtggacttta ctgtagacac taatggtgtt 2160 tatagtgaac ctcgccctat tggaacccgg tatctcacac gaaacttgta a 2211 // ID MT458148; SV 1; linear; other DNA; STD; SYN; 1905 BP. XX AC MT458148; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct EnvNS1 gene, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1905 RA Silva A.J.D., Jesus A.L.S., Freitas A.C.; RT "Development of vaccines against Zika virus based on epitopes of Envelope RT and NS1 proteins delivered by Pichia pastoris"; RL Unpublished. XX RN [2] RP 1-1905 RA Silva A.J.D., Jesus A.L.S., Freitas A.C.; RT ; RL Submitted (08-MAY-2020) to the INSDC. RL Genetics, Federal University of Pernambuco, Av. Prof. Moraes Rego, 1235, RL Recife, PE 50670-901, Brasil XX DR MD5; 55274ffa48d390fc07ab0c164e4d680e. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1905 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS <1..>1905 FT /codon_start=1 FT /transl_table=11 FT /product="EnvNS1" FT /protein_id="QLG96822.1" FT /translation="HHHHHHHHGGGGGGGGLQLDKQSDTQYVEAAAKEAAAKEAAAKEV FT QYAGTDGPCKEAAAKEAAAKEAAAKLVTCAKFACAEAAAKEAAAKALGLNTKNGSAEAA FT AKEAAAKALRLKGVSYSEAAAKEAAAKEAAAKRLITANPVIAEAAAKEAAAKANSPRAE FT ATLAAYMCLALGGVLAAYLQPGVQLTVVVGSAEAAAKEAAAKAVREDYSLECAEAAAKE FT AAAKAVKGKEAVHSEAAAKEAAAKEAAAKWRLKRAHLIEAAAKEAAAKEAAAKWYGMEI FT RPRAEAAAKEAAAKAKGPWHSEELAAYPGDDDDKSAKSSFISTTTTDLTSINTSAYSTG FT SISTVETGNRTTSEVISHVVTTSTKLSPTATTSLTIAQTSIYSTDSNITVGTDIHTTSE FT VISDVETISRETASTVVAAPTSTTGWTGAMNTYIPQFTSSSFATINSTPIISSSAVFET FT SDASIVNVHTENITNTAAVPSEEPTFVNATRNSLNSFCSSKQPSSPSSYTSSPLVSSLS FT VSKTLLSTSFTPSVPTSNTYIKTENTGYFEHTALTTSSVGLNSFSETALSSQGTKIDTF FT LVSSLIAYPSSASGSQLSGIQQNFTSTSLMISTYEGKASIFFSAELGSIIFLLLSYLLF" FT misc_feature <1..24 FT /note="Region: 8xHis affinity tag" FT misc_feature 25..48 FT /note="Region: Gly (8) linker" FT misc_feature 49..549 FT /note="Region: Env epitopes" FT misc_feature 550..930 FT /note="Region: NS1 epitopes" FT misc_feature 931..945 FT /note="Region: enterokinase site" FT misc_feature 946..>1905 FT /note="Region: alfa-agglutinin" XX SQ Sequence 1905 BP; 493 A; 494 C; 421 G; 497 T; 0 other; catcaccatc accatcatca ccatggtgga ggaggaggtg gaggtggtct gcagctggat 60 aaacagtcag atacccaata cgtggaagca gctgcaaaag aggctgctgc aaaggaggct 120 gctgccaagg aagtccaata tgctggtact gatggtccat gtaaggaggc agcagccaaa 180 gaggctgctg caaaggaagc cgctgctaag ttggttactt gcgctaagtt cgcctgcgct 240 gaggctgctg caaaggaggc tgccgcaaaa gccctgggat tgaacaccaa gaatggatct 300 gccgaagccg cagctaaaga agcagctgct aaggccttga ggttgaaagg agtttcatac 360 tctgaggcag cagccaaaga agctgctgca aaagaggcag ctgcaaagcg tcttatcact 420 gctaacccag ttatcgccga agcagccgct aaggaagcag ctgctaaggc aaattcccct 480 agagctgaag ctacacttgc tgcctacatg tgtcttgctc tgggaggagt tttggctgcc 540 tacctgcagc ccggggttca actgacagtg gttgttggat ctgccgaagc tgctgctaaa 600 gaagctgctg ctaaagctgt acgtgaggat tactctctgg aatgtgcaga agctgctgca 660 aaagaagctg ccgctaaagc cgttaaggga aaggaggctg tccattctga agccgcagct 720 aaagaagccg ctgctaaaga ggctgctgct aagtggaggt tgaagcgtgc tcacttgatc 780 gaagccgctg caaaagaggc agctgctaaa gaagcagccg caaagtggta tggaatggag 840 attaggccta gagcagaggc agccgctaaa gaggcagccg ctaaagctaa gggtccttgg 900 cactctgaag agttggctgc ttatcccggg gatgacgacg ataagtctgc taagtcttct 960 ttcatctcca ctactactac cgatcttact tctatcaata catccgctta ttccaccgga 1020 agtatttcta ccgtcgaaac cggaaacagg actacttccg aagttatctc ccacgtggtt 1080 accacttcta caaagttgtc cccaacagcc accacatctt taacaatagc tcagacctct 1140 atctactcaa ctgattccaa tatcaccgtt ggaactgaca tccacactac ttctgaggtg 1200 atttccgacg tcgaaactat ttctcgtgaa actgcttcta ctgttgtagc cgctccaact 1260 tctacaacag gatggaccgg agccatgaat acttacattc cccaattcac cagttcttct 1320 tttgcaacca tcaactctac ccctatcatc tcttcttctg ctgttttcga gacttctgat 1380 gccagtattg ttaacgtgca cactgagaat atcactaata cagccgcagt gccctctgag 1440 gaacccactt ttgttaatgc aaccaggaac tcactgaact ccttctgttc ctctaaacaa 1500 ccttcttccc ctagttcata cacctcatca ccattggttt ctagtttgtc tgtgtctaaa 1560 actctgcttt ctacttcctt cactccatct gttccaacca gtaatactta catcaagact 1620 gagaatactg gatactttga gcacactgcc ctgacaacct cttcagttgg actgaactca 1680 ttcagtgaga cagcactttc atctcaagga accaaaatcg atactttcct ggtatctagt 1740 ttgattgcct atccttcatc tgcttctggt tcccaattgt ctggtataca gcaaaacttt 1800 acttctactt ctttgatgat tagtacttac gaaggaaagg cttccatttt cttttctgct 1860 gagcttggat ctatcatctt cctgcttctt tcctaccttc tgttt 1905 // ID MT747949; SV 1; circular; other DNA; STD; SYN; 11657 BP. XX AC MT747949; XX DT 23-JUL-2020 (Rel. 144, Created) DT 23-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning vector pWattB-nlacZ, complete sequence. XX KW . XX OS Cloning vector pWattB-nlacZ OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-11657 RX PUBMED; 26657774. RA Ahmad SM, Bhattacharyya P, Jeffries N, Gisselbrecht SS Michelson.AM.; RT "Two Forkhead transcription factors regulate cardiac progenitor RT specification by controlling the expression of receptors of the fibroblast RT growth factor and Wnt signaling pathways"; RL Development 143(2):306-317(2016). XX RN [2] RP 1-11657 RA Panta M., Kump A.J., Dalloul J.M., Schwab K.R., Ahmad S.M.; RT "Three distinct mechanisms, Notch instructive, permissive, and independent, RT regulate the expression of two different pericardial genes to specify RT cardiac cell subtypes"; RL Unpublished. XX RN [3] RP 1-11657 RA Ahmad S.M.; RT ; RL Submitted (12-JUL-2020) to the INSDC. RL Department of Biology, Indiana State University, Science Building, Room RL 287H, 600 Chestnut St., Terre Haute, IN 47809, USA XX DR MD5; 1950fc8e0a5a906b171a267aaa0e7075. XX CC ##Assembly-Data-START## CC Assembly Method :: FlyE v. 2020-03-20 CC Sequencing Technology :: PacBio CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..11657 FT /organism="Cloning vector pWattB-nlacZ" FT /mol_type="other DNA" FT /db_xref="taxon:2754839" FT misc_recomb 89..373 FT /note="phiC31 attB site; derived from Streptomyces FT lividans" FT gene complement(1423..2283) FT /gene="AmpR" FT CDS complement(1423..2283) FT /codon_start=1 FT /transl_table=11 FT /gene="AmpR" FT /product="beta-lactamase" FT /note="confers resistance to ampicillin, carbenicillin, and FT related antibiotics" FT /protein_id="QLI61520.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT gene complement(2574..5446) FT /gene="mini-white" FT CDS complement(join(2574..3188,3259..3390,3594..3909, FT 3971..4625,4700..4973,5375..5446)) FT /codon_start=1 FT /transl_table=11 FT /gene="mini-white" FT /product="white" FT /note="derived from Drosophila; lacks part of the first FT intron" FT /protein_id="QLI61519.1" FT /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY FT GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH FT IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG FT QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL FT SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ FT VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP FT TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP FT ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG FT VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL FT FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV FT GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS FT NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE" FT regulatory 6097..6491 FT /note="gypsy" FT /regulatory_class="insulator" FT misc_feature 6496..6586 FT /note="multiple cloning site" FT regulatory 6596..6687 FT /note="Hsp27" FT /regulatory_class="TATA_box" FT gene 6735..9833 FT /gene="lacZ" FT CDS 6735..9833 FT /codon_start=1 FT /transl_table=11 FT /gene="lacZ" FT /product="beta-galactosidase" FT /note="derived from Escherichia coli; contains nuclear FT localization signal" FT /protein_id="QLI61521.1" FT /translation="MGDPTPPKKKRKVEDDPVVLQRRDWENPGVTQLNRLAAHPPFASW FT RNSEEARTDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGY FT DAPIYTNVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWC FT NGRWVGYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVS FT LLHKPTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTA FT PFGGEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVG FT FREVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCS FT HYPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN FT HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDE FT DQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFV FT WDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQF FT FQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPEL FT PQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSE FT MDFCIELGNKRWQFNRQSGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDP FT NAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSG FT QMAITVDVEVASDTPHPARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLP FT LSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEE FT GTWLNIDGFHMGIGGDDSWSPSVSAELQLSAGRYHYQLVWCQK" FT regulatory 10987..11373 FT /note="gypsy" FT /regulatory_class="insulator" XX SQ Sequence 11657 BP; 3006 A; 2858 C; 2854 G; 2939 T; 0 other; agcgtcaatg tccgccttca gttgcatttt gtcagcggtt tcgtgacgaa gctccaagcg 60 gtttacgcca tcaattaaac acggccgcgt cgacgatgta ggtcacggtc tcgaagccgc 120 ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc tcacccatct 180 ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac gcgcggcgca 240 ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc acgggacgtg 300 cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg acggtcacgg 360 cgggcatgtc gactcgaggc cggtctccct atagtgagtc gtattaattt cgataagcca 420 ggttaacctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 480 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 540 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 600 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 660 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 720 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 780 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 840 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 900 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 960 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 1020 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 1080 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 1140 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 1200 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 1260 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 1320 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 1380 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 1440 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 1500 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 1560 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 1620 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 1680 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 1740 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 1800 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 1860 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 1920 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 1980 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 2040 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 2100 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 2160 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 2220 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 2280 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 2340 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 2400 aaaagtgcca cctgacgtct tcgaagggga aatacttgta ttctataggt catatcttgt 2460 ttttattggc acaaatataa ttacattagc tttttgaggg ggcaataaac agtaaacacg 2520 atggtaataa tggtaaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc 2580 ttgcgtcggg cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga 2640 atggccagac ccacgtagtc cagcggcaga tcggcggcgg agaagttaag cgtctccagg 2700 atgaccttgc ccgaactggg gcacgtggtg ttcgacgatg tgcagctaat ttcgcccggc 2760 tccacgtccg cccattggtt aatcagcaga ccctcgttgg cgtaacggaa ccatgagagg 2820 tacgacaacc atttgaggta tactggcacc gagcccgagt tcaagaagaa gccgccaaag 2880 agcaggaatg gtatgataac cggcggaccc acagacagcg ccatcgaggt cgaggagctg 2940 gcgcaggata ttagatatcc gaaggacgtt gacacattgg ccaccagagt gaccagcgcc 3000 aggcagttga agaagtgcag cactccggcc cgcagtccga tcatcggata ggcaatcgcc 3060 gtgaagacca gtggcactgt gagaaaaagc ggcaattcgg caatcgtttt gcccagaaag 3120 tatgtgtcac agcgataaag tcgacttcgg gcctccctca taaaaactgg cagctctgag 3180 gtgaacacct aaatcgaatc gattcattag aaagttagta aattattgaa atgcaaatgt 3240 attctaaaca tgacttacat ttatcgtggc aaagacgttt tgaaaggtca tgttggtcag 3300 gaagaggaag atggctccgt tgatattcat cacacccact tgcgtgagtt gttggcccaa 3360 aaagatgagg ccaatcaaga tggcaaccat ctgcaaatta aaatgttact cgcatctcat 3420 taatattcgc gagttaaatg aaatttattt atcttctgca aaactataaa ctatacatct 3480 cattgaaaaa aactaagaag ggtgtggaat caggcaattc tatctaaaat ctagcgaatt 3540 tgtttccaag aattgtaagc gttatatcat ttgtttccac tggaaccact caccgttgtc 3600 tgaataagtc gcacttttac gaggagtggt tccttgagca ccgacagcca ggatcgccac 3660 aggaccgccc ggaactgcat gaaccaggtg gccttgtagg tgtacccatt ctccggctgc 3720 tccagtggct tctccagatt tttggtggcc aacaactgct ccatatcccg ggctactttg 3780 ctaatggcaa aattgtcgca tatcttggcg atccgatcac gggactcgat ctcccgtccg 3840 ggcacaacgg ccaacacctg tacgtaaaag tccgccggat tgtagttggt aggacactgg 3900 gcacccacgc tggataggag ttgagatgtt atgtaatact agataccctt aataaacaca 3960 tcgaactcac taggaaaaga agtcgacggc ttcgctggga gtgcccaaga aagctaccct 4020 gccctcggcc atcagaagga tcttgtcaaa gagctcaaac agctcggaag acggctgatg 4080 aatggtcagg atgacggtct tgcccttctg cgacagcttc ttcagcacct ggacgacgct 4140 gtgggcggta aaggagtcca gtccggaggt gggctcatcg cagatcagaa gcggcggatc 4200 ggttagagcc tcggaggcga atgccagacg cttcctttct ccgccggaca gacctttcac 4260 cctgccgggc acaccgatga tcgtgtgctg acatttgctg agcgaaagct cctggatcac 4320 ctgatccacg cgggccactc gctgccgata ggtcagatgt cgtggcatcc gcaccatggc 4380 ttggaaaatc aggtgttccc tggccgttag ggagccgata aagaggtcat cctgctggac 4440 ataggcgcac ctggcctgca tctccttggc gtccacaggt tggccattga gcagtcgcat 4500 cccggatggc gatacttgga tgccctgcgg cgatcgaaag gcaagggcat tcagcagggt 4560 cgtctttccg gcaccggaac tgcccatcac ggccaaaagt tcgcccggat aggccacgcc 4620 gcaaactgag tttcaaattg gtaattggac cctttattaa gatttcacac agatcagccg 4680 actgcgaata gaaactcacc gttcttgagc aaatgtttcc tgggcgccgg tatgtgtcgc 4740 tcgttgcaga atagtccgcg tgtccggttg accagctgcc gccatccgga gcccggctga 4800 ttgaccgccc caaagatgtc catattgtgc caggcatagg tgaggttctc ggctagttgg 4860 ccgctccctg aaccggagtc ctccggcgga ctgggtggcc ggagcgtgcc gtagtttttg 4920 gcctgcccga agccctggtt aatgcagctc tgcgaagccg ctccgctgtc accctgcaat 4980 gataggggat ctcaaatatc aactacaagc gttatgctca tctaaccccg aacaaaaagt 5040 accccgaagt atcctacgaa gtaggtttat acttttattt attttttgtg catctaggat 5100 cagcttaaaa tatctggttg ttatattttt tgtaaaaaag aatatagtcg aaaatgaatg 5160 cctttagatg tcttgatcat gatatgatct caaaaattgt cttatatagc gagaacagct 5220 accagaataa tctgtttcgt gtcactattt gtttgtgcaa ttgcggtttg ggatttttgt 5280 gggtcgcagt tctcacgccg cagacaattt gatgttgcaa tcgcagttcc tatagatcaa 5340 gtgaacttaa gatgtatgca catgtactac tcacattgtt cagatgctcg gcagatgggt 5400 gtttgctgcc tccgcgaatt aatagctcct gatcctcttg gcccattgcc gggatttttc 5460 acactttccc ctgcttaccc acccaaaacc aatcaccacc ccaatcactc aaaaaacaaa 5520 caaaaataag aagcgagagg agttttggca cagcactttg tgtttaattg atggcgtaaa 5580 ccgcttggag cttcgtcacg aaaccgctga caaaatgcaa ctgaaggcgg acattgacgc 5640 tacgtaacgc tacaaacggt ggcgaaagag atagcggacg cagcggcgaa agagacggcg 5700 atatttctgt ggacagagaa ggaggcaaac agcccggacg tctaagaaac cattattatc 5760 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt 5820 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa 5880 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg 5940 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatggacata 6000 ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca catacgattt 6060 aggtgacact atagaaccag atcagcttgg ctgcatcacg taataagtgt gcgttgaatt 6120 tattcgcaaa aacattgcat attttcggca aagtaaaatt ttgttgcata ccttatcaaa 6180 aaataagtgc tgcatacttt ttagagaaac caaataattt tttattgcat acccgttttt 6240 aataaaatac attgcatacc ctcttttaat aaaaaatatt gcatactttg acgaaacaaa 6300 ttttcgttgc atacccaata aaagattatt atattgcata cccgttttta ataaaataca 6360 ttgcataccc tcttttaata aaaaatattg catacgttga cgaaacaaat tttcgttgca 6420 tacccaataa aagattatta tattgcatac cttttcttgc cataccattt agccgatcaa 6480 ttgtgctcgg caacagcatg ctgcagcaga tctggtctag agcccgggcg aattcgccgg 6540 cgcgccggta ccccgcggcc gctagcggat ccgcggccgc tacgtatcta gagtcgagcc 6600 cagcgtcagt ataaaagccg gcgtcaacgt cgaccgagca cagtctaaac tgaaaaattg 6660 aaggcaaacg ttgaagcaaa cttcgctaaa aaaaaattcg aaaaggcaaa aaaaattcct 6720 ttgtctagac caccatggga gatcccaccc cacccaagaa gaagcgcaag gtggaggacg 6780 atcccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc 6840 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc 6900 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag 6960 aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc 7020 cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgtg acctatccca 7080 ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat 7140 ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgtta 7200 actcggcgtt tcatctgtgg tgcaacgggc gctgggtcgg ttacggccag gacagtcgtt 7260 tgccgtctga atttgacctg agcgcatttt tacgcgccgg agaaaaccgc ctcgcggtga 7320 tggtgctgcg ctggagtgac ggcagttatc tggaagatca ggatatgtgg cggatgagcg 7380 gcattttccg tgacgtctcg ttgctgcata aaccgactac acaaatcagc gatttccatg 7440 ttgccactcg ctttaatgat gatttcagcc gcgctgtact ggaggctgaa gttcagatgt 7500 gcggcgagtt gcgtgactac ctacgggtaa cagtttcttt atggcagggt gaaacgcagg 7560 tcgccagcgg caccgcgcct ttcggcggtg aaattatcga tgagcgtggt ggttatgccg 7620 atcgcgtcac actacgtctg aacgtcgaaa acccgaaact gtggagcgcc gaaatcccga 7680 atctctatcg tgcggtggtt gaactgcaca ccgccgacgg cacgctgatt gaagcagaag 7740 cctgcgatgt cggtttccgc gaggtgcgga ttgaaaatgg tctgctgctg ctgaacggca 7800 agccgttgct gattcgaggc gttaaccgtc acgagcatca tcctctgcat ggtcaggtca 7860 tggatgagca gacgatggtg caggatatcc tgctgatgaa gcagaacaac tttaacgccg 7920 tgcgctgttc gcattatccg aaccatccgc tgtggtacac gctgtgcgac cgctacggcc 7980 tgtatgtggt ggatgaagcc aatattgaaa cccacggcat ggtgccaatg aatcgtctga 8040 ccgatgatcc gcgctggcta ccggcgatga gcgaacgcgt aacgcgaatg gtgcagcgcg 8100 atcgtaatca cccgagtgtg atcatctggt cgctggggaa tgaatcaggc cacggcgcta 8160 atcacgacgc gctgtatcgc tggatcaaat ctgtcgatcc ttcccgcccg gtgcagtatg 8220 aaggcggcgg agccgacacc acggccaccg atattatttg cccgatgtac gcgcgcgtgg 8280 atgaagacca gcccttcccg gctgtgccga aatggtccat caaaaaatgg ctttcgctac 8340 ctggagagac gcgcccgctg atcctttgcg aatacgccca cgcgatgggt aacagtcttg 8400 gcggtttcgc taaatactgg caggcgtttc gtcagtatcc ccgtttacag ggcggcttcg 8460 tctgggactg ggtggatcag tcgctgatta aatatgatga aaacggcaac ccgtggtcgg 8520 cttacggcgg tgattttggc gatacgccga acgatcgcca gttctgtatg aacggtctgg 8580 tctttgccga ccgcacgccg catccagcgc tgacggaagc aaaacaccag cagcagtttt 8640 tccagttccg tttatccggg caaaccatcg aagtgaccag cgaatacctg ttccgtcata 8700 gcgataacga gctcctgcac tggatggtgg cgctggatgg taagccgctg gcaagcggtg 8760 aagtgcctct ggatgtcgct ccacaaggta aacagttgat tgaactgcct gaactaccgc 8820 agccggagag cgccgggcaa ctctggctca cagtacgcgt agtgcaaccg aacgcgaccg 8880 catggtcaga agccgggcac atcagcgcct ggcagcagtg gcgtctggcg gaaaacctca 8940 gtgtgacgct ccccgccgcg tcccacgcca tcccgcatct gaccaccagc gaaatggatt 9000 tttgcatcga gctgggtaat aagcgttggc aatttaaccg ccagtcaggc tttctttcac 9060 agatgtggat tggcgataaa aaacaactgc tgacgccgct gcgcgatcag ttcacccgtg 9120 caccgctgga taacgacatt ggcgtaagtg aagcgacccg cattgaccct aacgcctggg 9180 tcgaacgctg gaaggcggcg ggccattacc aggccgaagc agcgttgttg cagtgcacgg 9240 cagatacact tgctgatgcg gtgctgatta cgaccgctca cgcgtggcag catcagggga 9300 aaaccttatt tatcagccgg aaaacctacc ggattgatgg tagtggtcaa atggcgatta 9360 ccgttgatgt tgaagtggcg agcgatacac cgcatccggc gcggattggc ctgaactgcc 9420 agctggcgca ggtagcagag cgggtaaact ggctcggatt agggccgcaa gaaaactatc 9480 ccgaccgcct tactgccgcc tgttttgacc gctgggatct gccattgtca gacatgtata 9540 ccccgtacgt cttcccgagc gaaaacggtc tgcgctgcgg gacgcgcgaa ttgaattatg 9600 gcccacacca gtggcgcggc gacttccagt tcaacatcag ccgctacagt caacagcaac 9660 tgatggaaac cagccatcgc catctgctgc acgcggaaga aggcacatgg ctgaatatcg 9720 acggtttcca tatggggatt ggtggcgacg actcctggag cccgtcagta tcggcggaat 9780 tacagctgag cgccggtcgc taccattacc agttggtctg gtgtcaaaaa taataataac 9840 cgggcaggcc atgtctgccc gtatttcgcg taaggaaatc cattatgtac tatttaaaaa 9900 acacaaactt ttggatgttc ggtttattct ttttctttta cttttttatc atgggagcct 9960 acttcccgtt tttcccgatt tggctacatg acatcaacca tatcagcaaa agtgatacgg 10020 gtattatttt tgccgctatt tctctgttct cgctattatt ccaaccgctg tttggtctgc 10080 tttctgacaa actcggcctc gactctagct agaggatctt tgtgaaggaa ccttacttct 10140 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa 10200 aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta ttttagattc 10260 caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt 10320 tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct caacattcta 10380 ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca gaattgctaa 10440 gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct atttacacca 10500 caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct gtaaccttta 10560 taagtaggca taacagttat aatcataaca tactgttttt tcttactcca cacaggcata 10620 gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt ttaatttgta 10680 aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat aatcagccat 10740 accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 10800 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 10860 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 10920 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcgggcg agctcgaatt 10980 gactagtcac gtaataagtg tgcgttgaat ttattcgcaa aaacattgca tattttcggc 11040 aaagtaaaat tttgttgcat accttatcaa aaaataagtg ctgcatactt tttagagaaa 11100 ccaaataatt ttttattgca tacccgtttt taataaaata cattgcatac cctcttttaa 11160 taaaaaatat tgcatacttt gacgaaacaa attttcgttg catacccaat aaaagattat 11220 tatattgcat acccgttttt aataaaatac attgcatacc ctcttttaat aaaaaatatt 11280 gcatacgttg acgaaacaaa ttttcgttgc atacccaata aaagattatt atattgcata 11340 ccttttcttg ccataccatt tagccgatca attctagtat gtatgtaagt taataaaacc 11400 catttttgcg gaaagtagat aaaaaaaaca tttttttttt ttactgcact ggatatcatt 11460 gaacttatct gatcagtttt aaatttactt cgatccaagg gtatttgatg taccaggttc 11520 tttcgattac ctctcactca aaatgacatt ccactcaaag tcagcgctgt ttgcctcctt 11580 ctctgtccac agaaatatcg ccgtctcttt cgccgctgcg tccgctatct ctttcgccac 11640 cgtttgtagc gttacgt 11657 // ID MT344942; SV 1; circular; other DNA; STD; SYN; 7205 BP. XX AC MT344942; XX DT 20-JUL-2020 (Rel. 144, Created) DT 20-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Vector pMQ650, complete sequence. XX KW . XX OS Vector pMQ650 OC other sequences; artificial sequences; vectors. XX RN [1] RC Publication Status: Online-Only RP 1-7205 RX PUBMED; 32414795. RA Callaghan J.D., Stella N.A., Lehner K.M., Treat B.R., Brothers K.M., RA St Leger A.J., Shanks R.M.Q.; RT "Xylose-Inducible Promoter Tools for Pseudomonas Species and Their Use in RT Implicating a Role for the Type II Secretion System Protein XcpQ in the RT Inhibition of Corneal Epithelial Wound Closure"; RL Appl. Environ. Microbiol. 86(14):e00250-20(2020). XX RN [2] RP 1-7205 RA Shanks R.M.Q., Lehner K.M.; RT ; RL Submitted (15-APR-2020) to the INSDC. RL Ophthalmology, University of Pittsburgh, 203 Lothrop St, Pittsburgh, PA RL 15213, USA XX DR MD5; 6fa0a8de3d728ad5d12dffbaed357562. XX CC ##Assembly-Data-START## CC Assembly Method :: RAST v. tk CC Coverage :: 32 CC Sequencing Technology :: PacBio CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..7205 FT /organism="Vector pMQ650" FT /mol_type="other DNA" FT /db_xref="taxon:2748649" FT regulatory complement(51..412) FT /regulatory_class="terminator" FT regulatory complement(452..463) FT /note="stop codons" FT /regulatory_class="other" FT regulatory complement(517..523) FT /regulatory_class="ribosome_binding_site" FT repeat_region 523..548 FT /rpt_family="operator" FT /rpt_type=DIRECT FT gene 700..1875 FT /gene="xutR" FT /note="from Pseudomonas fluorescens PF0-1" FT CDS 700..1875 FT /codon_start=1 FT /transl_table=11 FT /gene="xutR" FT /product="XutR" FT /note="xylose responsive transcriptional regulator" FT /protein_id="QLG03807.1" FT /translation="MKTVPPVHRIALLFNGSKIYDRGIISGIGNYLSSTRASWDLFLEE FT DFLCRLKGIERWQGDGIIADFDDPLIGEALADIKLPVVAVGGSYQDERAYPKGIPYVAT FT DNNALITMAYEHLIEAGLQRFACFSLPEAQANRWAQEREKAFLRLMQRDGSHGEVYRGM FT GTSAPLWDSAVEQLIAWLQSLPKPIGIIAVSDARARQLLQACLTAGIAVPEQVALIGID FT NDPLTRSLTRVPLSSVIQGTETMGRTAAQLLHQMLHGKPSTGAHFLIPPEAINVQVSSL FT HQPLGNPYVMQALLFIRQYACQGIKTAQVAAYVGVSRSSLESHFRAVRGCSVHDEILRF FT KLAAATRGLEKTDTAIADVARTCGFKSAQYLHTVFRREFGCTPREYQQGAR" FT regulatory 1983..2842 FT /note="oripBBR1" FT /regulatory_class="replication_regulatory_region" FT gene 2843..3505 FT /gene="rep" FT CDS 2843..3505 FT /codon_start=1 FT /transl_table=11 FT /gene="rep" FT /product="Rep" FT /note="replication protein for pBBR1 origin of replication" FT /protein_id="QLG03808.1" FT /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH FT HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV FT NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE FT PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR" FT gene complement(3786..4319) FT /gene="aacC-1" FT CDS complement(3786..4319) FT /codon_start=1 FT /transl_table=11 FT /gene="aacC-1" FT /product="AacC-1" FT /note="Gentamicin resistance protein" FT /protein_id="QLG03809.1" FT /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD FT LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS FT EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR FT EEVMHFDIDPSTAT" FT gene complement(4755..5558) FT /gene="ura3" FT CDS complement(4755..5558) FT /codon_start=1 FT /transl_table=11 FT /gene="ura3" FT /product="Ura3" FT /note="Uracil biosynthetic protein from Saccharomyces FT cerevisiae" FT /protein_id="QLG03810.1" FT /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL FT ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ FT YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE FT YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD FT VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" FT regulatory 5792..6305 FT /regulatory_class="replication_regulatory_region" FT regulatory 6399..7205 FT /note="RP4 oriT" FT /regulatory_class="other" XX SQ Sequence 7205 BP; 1687 A; 1977 C; 1860 G; 1681 T; 0 other; catatttgaa tgtatttaga aaaataaaca aaagagtttg tagaaacgca aaaaggccat 60 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc 120 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac 180 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag 240 cctttcgttt tatttgatgc ctggcagttc cctactctcg catggggaga ccccacacta 300 ccatcggcgc tacggcgttt cacttctgag ttcggcatgg ggtcaggtgg gaccaccgcg 360 ctactgccgc caggcaaatt ctgttttatc agaccgcttc tgcgttctga tttaatctgt 420 atcaggctga aaatcttctc tcatccgcca attattctat ttaccaagct tgcatgcgac 480 tagttggatc cttgggtcga ccgaagaatt caaaaatcct ccttgttgtt cttggttgtt 540 gttcttgggc gtgtcccgat actagcaacg gccctgtgcc tgccgattac gaaaatcacc 600 aacacgcagt gcgattttgc gtattgagat tcgccctctg cgcgcctagt ctgtgcagac 660 cgcctctacg gcaagaggcc cggaacaagc ctgaaaacaa tgaaaaccgt accgcctgtt 720 caccgcatcg ccctgttgtt caacggcagc aagatctatg accgcggcat catcagcggc 780 atcggcaact acctgagcag cacgcgcgcg tcttgggacc tgtttctgga agaggatttt 840 ctctgccgct tgaaaggtat cgagcgttgg caaggtgacg ggatcatcgc cgacttcgac 900 gatccgctga ttggcgaggc gctggccgat atcaagttgc ccgtggtagc ggtgggtggt 960 tcttatcagg atgagcgggc ctatcccaaa ggcatcccgt acgtcgcgac cgacaataac 1020 gcgctgatca ccatggctta cgagcacttg atcgaggccg ggttgcaacg gtttgcctgt 1080 ttcagcctgc cggaggcgca ggccaaccgc tgggcccagg aacgcgaaaa ggcttttctg 1140 cgattgatgc aacgggacgg ctcgcacggc gaggtctatc gcggcatggg caccagcgcg 1200 ccgctgtggg acagcgccgt cgaacagttg atcgcctggc tgcaaagcct gcccaagccc 1260 attggcatca tcgcggtcag tgacgcccgt gcccggcagt tgctgcaagc ctgcctgacc 1320 gccggcatcg ccgttccgga acaggtggcg ttgatcggca tcgacaacga cccactgacc 1380 cgcagcctga cccgggtccc gctgagttcg gtgattcagg gcaccgaaac catgggccgc 1440 accgccgcgc aactgctgca ccagatgctg cacggcaaac cgtcaacggg cgcgcatttc 1500 ctgatcccgc ccgaagcgat caacgtgcag gtgtcgagct tgcatcaacc gttgggcaac 1560 ccctacgtga tgcaggcgct gctgttcatc cgccaatatg cctgtcaggg catcaagacc 1620 gcgcaagtgg cggcctatgt cggtgtgtcg cgctcctccc tggaatcgca ctttcgcgcg 1680 gtgcgtggtt gcagcgtgca tgacgagatt ctgcgtttca aactggcggc ggccactcgc 1740 ggactggaaa aaaccgacac agcgattgcg gacgttgccc gcacgtgcgg tttcaaatcc 1800 gcacagtacc tgcacacggt gtttcgccgg gagttcgggt gtacgccccg tgaatatcag 1860 cagggcgccc gatagccggc cactcaaaag cgatccagcc gatagggctc gctcgacggg 1920 agaatacgcg ccgtgtcctg ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 1980 atgcgcccta cgggcttgct ctccgggctt cgccctgcgc ggccgctgcg ctcccttgcc 2040 agcccgtgga tatgtggacg atggccgcga gcggccaccg gctggctcgc ttcgctcggc 2100 ccgtggacaa ccctgctgga caagctgatg gacaggctgc gcctgcccac gagcttgacc 2160 acagggattg cccaccggct acccagcctt cgaccacata cccaccggct ccaactgcgc 2220 ggcctgcggc cttgccccat caattttttt aattttctct ggggaaaagc ctccggcctg 2280 cggcctgcgc gcttcgcttg ccggttggac accaagtgga aggcgggtca aggctcgcgc 2340 agcgaccgcg cagcggcttg gccttgacgc gcctggaacg acccaagcct atgcgagtgg 2400 gggcagtcga agggcgaagc ccgcccgcct gccccccgag cctcacggcg gcgagtgcgg 2460 gggttccaag ggggcagcgc caccttgggc aaggccgaag gccgcgcagt cgatcaacaa 2520 gccccggagg ggccactttt tgccggaggg ggagccgcgc cgaaggcgtg ggggaacccc 2580 gcaggggtgc ccttctttgg gcaccaaaga actagatata gggcgaaatg cgaaagactt 2640 aaaaatcaac aacttaaaaa aggggggtac gcaacagctc attgcggcac cccccgcaat 2700 agctcattgc gtaggttaaa gaaaatctgt aattgactgc cacttttacg caacgcataa 2760 ttgttgtcgc gctgccgaaa agttgcagct gattgcgcat ggtgccgcaa ccgtgcggca 2820 cccctaccgc atggagataa gcatggccac gcagtccaga gaaatcggca ttcaagccaa 2880 gaacaagccc ggtcactggg tgcaaacgga acgcaaagcg catgaggcgt gggccgggct 2940 tattgcgagg aaacccacgg cggcaatgct gctgcatcac ctcgtggcgc agatgggcca 3000 ccagaacgcc gtggtggtca gccagaagac actttccaag ctcatcggac gttctttgcg 3060 gacggtccaa tacgcagtca aggacttggt ggccgagcgc tggatctccg tcgtgaagct 3120 caacggcccc ggcaccgtgt cggcctacgt ggtcaatgac cgcgtggcgt ggggccagcc 3180 ccgcgaccag ttgcgcctgt cggtgttcag tgccgccgtg gtggttgatc acgacgacca 3240 ggacgaatcg ctgttggggc atggcgacct gcgccgcatc ccgaccctgt atccgggcga 3300 gcagcaacta ccgaccggcc ccggcgagga gccgcccagc cagcccggca ttccgggcat 3360 ggaaccagac ctgccagcct tgaccgaaac ggaggaatgg gaacggcgcg ggcagcagcg 3420 cctgccgatg cccgatgagc cgtgttttct ggacgatggc gagccgttgg agccgccgac 3480 acgggtcacg ctgccgcgcc ggtagcactt gggttgcgca gcaacccgta agtgcgctgt 3540 tccagactat cggctgtagc cgcctcgccg ccctatacct tgtctgcctc cccgcgttgc 3600 gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccttttac gagtttttgg 3660 tgcaatcaat ctaaagtata tatgagtaaa cttggtctga caatcgatgc gaattggccg 3720 cggcgttgtg acaatttacc gaacaactcc gcggccggga agccgatctc ggcttgaacg 3780 aattgttagg tggcggtact tgggtcgata tcaaagtgca tcacttcttc ccgtatgccc 3840 aactttgtat agagagccac tgcgggatcg tcaccgtaat ctgcttgcac gtagatcaca 3900 taagcaccaa gcgcgttggc ctcatgcttg aggagattga tgagcgcggt ggcaatgccc 3960 tgcctccggt gctcgccgga gactgcgaga tcatagatat agatctcact acgcggctgc 4020 tcaaacttgg gcagaacgta agccgcgaga gcgccaacaa ccgcttcttg gtcgaaggca 4080 gcaagcgcga tgaatgtctt actacggagc aagttcccga ggtaatcgga gtccggctga 4140 tgttgggagt aggtggctac gtctccgaac tcacgaccga aaagatcaag agcagcccgc 4200 atggatttga cttggtcagg gccgagccta catgtgcgaa tgatgcccat acttgagcca 4260 cctaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct gcgtaacatc 4320 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc 4380 ccgaggcata gactgtacaa aaaaacagtc ataacaagcc atgaaaaccg ccactgcgcc 4440 gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg cgtgagcgca tacgctactt 4500 gcattacagt ttacgaaccg aacaggctta tgtcaattcg gttgaatact catactcttc 4560 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 4620 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgggt 4680 aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca tgcatttact 4740 tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc ccagcctgct 4800 tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag tcctcttcca 4860 acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata ctgttgaccc 4920 aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca atcgtaacct 4980 tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc tttgtcgctc 5040 ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc cttgcatgac 5100 aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc cgcctgcttc 5160 aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc tgcccattct 5220 gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat gtcagcaaat 5280 tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag cggcttaact 5340 gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa acaaattttg 5400 ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc caatgaagca 5460 cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac aggactagga 5520 tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg tttcctgcag 5580 gtttttgttc tgtgcagttg ggttaagaat actgggcaat ttcatgtttc ttcaacacta 5640 catatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc cttctgttcg 5700 gagattaccg aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga ataaaaaaaa 5760 aatgatgaat tgaaaagcta gcttatcgat gggtcctttt catcacgtgc tataaaaata 5820 attataattt aaatttttta atataaatat ataaattaaa aatagaaagt aaaaaaagaa 5880 attaaagaaa aaatagtttt tgttttccga agatgtaaaa gactctaggg ggatcgccaa 5940 caaatactac cttttatctt gctcttcctg ctctcaggta ttaatgccga attgtttcat 6000 cttgtctgtg tagaagacca cacacgaaaa tcctgtgatt ttacatttta cttatcgtta 6060 atcgaatgta tatctattta atctgctttt cttgtctaat aaatatatat gtaaagtacg 6120 ctttttgttg aaatttttta aacctttgtt tatttttttt tcttcattcc gtaactcttc 6180 taccttcttt atttactttc taaaatccaa atacaaaaca taaaaataaa taaacacaga 6240 gtaaattccc aaattattcc atcattaaaa gatacgaggc gcgtgtaagt tacaggcaag 6300 cgatctctaa gaaaccatta ttatcatgac attaacctat aaaaaaggcc tctcgagcta 6360 gaatcgatct tcgccagcag ggcgaggatc gtggcatcac cgaaccgcgc cgtgcgcggg 6420 tcgtcggtga gccagagttt cagcaggccg cccaggcggc ccaggtcgcc attgatgcgg 6480 gccagctcgc ggacgtgctc atagtccacg acgcccgtga ttttgtagcc ctggccgacg 6540 gccagcaggt aggccgacag gctcatgccg gccgccgccg ccttttcctc aatcgctctt 6600 cgttcgtctg gaaggcagta caccttgata ggtgggctgc ccttcctggt tggcttggtt 6660 tcatcagcca tccgcttgcc ctcatctgtt acgccggcgg tagccggcca gcctcgcaga 6720 gcaggattcc cgttgagcac cgccaggtgc gaataaggga cagtgaagaa ggaacacccg 6780 ctcgcgggtg ggcctacttc acctatcctg cccggctgac gccgttggat acaccaagga 6840 aagtctacac gaaccctttg gcaaaatcct gtatatcgtg cgaaaaagga tggatatacc 6900 gaaaaaatcg ctataatgac cccgaagcag ggttatgcag cggaaaagcg ctgcttccct 6960 gctgttttgt ggaatatcta ccgactggaa acaggcaaat gcaagaaatt actgaactga 7020 ggggacaggc gagaggcgat gccaaagagc tacaccgacg agctggccga gtgggttgaa 7080 tcccgcgcgg ccaagaagcg ccggcgtgat gaggctgcgg ttgcgttcct ggcggtgagg 7140 gcggatgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 7200 aggcg 7205 // ID MT345687; SV 1; circular; other DNA; STD; SYN; 5505 BP. XX AC MT345687; XX DT 20-JUL-2020 (Rel. 144, Created) DT 20-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning vector pALH015, complete sequence. XX KW . XX OS Cloning vector pALH015 OC other sequences; artificial sequences; vectors. XX RN [1] RC Publication Status: Available-Online prior to print RP 1-5505 RA Herneisen A.L., Sidik S.M., Markus B.M., Drewry D.H., Zuercher W.J., RA Lourido S.; RT "Identifying the Target of an Antiparasitic Compound in Toxoplasma Using RT Thermal Proteome Profiling"; RL ACS Chem. Biol.:(2020). XX RN [2] RP 1-5505 RA Herneisen A.L., Sidik S.M., Markus B.M., Zuercher W.J., Lourido S.; RT ; RL Submitted (15-APR-2020) to the INSDC. RL Whitehead Institute, Massachusetts Institute of Technology, 455 Main St, RL Cambridge, MA 02142, USA XX DR MD5; ce5f1172fb8da2a6ab3e0329dbccc394. XX FH Key Location/Qualifiers FH FT source 1..5505 FT /organism="Cloning vector pALH015" FT /mol_type="other DNA" FT /db_xref="taxon:2750731" FT rep_origin complement(3..458) FT /note="f1 ori; f1 bacteriophage origin of replication" FT primer_bind 600..616 FT /note="M13 fwd" FT regulatory 626..644 FT /note="T7 promoter" FT /regulatory_class="promoter" FT primer_bind 670..686 FT /note="KS" FT 5'UTR 698..1195 FT /note="TgTUB1 5'UTR" FT CDS 1196..2548 FT /codon_start=1 FT /transl_table=11 FT /product="GCaMP6f" FT /note="fluorescent protein-based calcium sensor" FT /protein_id="QLF98886.1" FT /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK FT TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP FT VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE FT LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT FT YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE FT LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS FT LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN FT GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK" FT 3'UTR 2549..3291 FT /note="3'UTR of DHFR" FT regulatory complement(3317..3335) FT /note="T3 promoter" FT /regulatory_class="promoter" FT primer_bind complement(3356..3372) FT /note="M13 rev" FT protein_bind 3380..3396 FT /bound_moiety="lac repressor" FT regulatory complement(3404..3434) FT /note="E. coli lac operon promoter" FT /regulatory_class="promoter" FT protein_bind 3449..3470 FT /bound_moiety="catabolite activator protein" FT rep_origin complement(3758..4346) FT /note="high-copy-number ColE1/pMB1/pBR322/pUC" FT gene complement(4517..5377) FT /gene="bla" FT CDS complement(4517..5377) FT /codon_start=1 FT /transl_table=11 FT /gene="bla" FT /product="beta-lactamase" FT /note="confers resistance to ampicillin, carbenicillin, and FT related antibiotics" FT /protein_id="QLF98887.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT regulatory complement(4517..5377) FT /gene="bla" FT /note="ampR promoter" FT /regulatory_class="promoter" XX SQ Sequence 5505 BP; 1378 A; 1403 C; 1462 G; 1261 T; 1 other; ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 60 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 120 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 180 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 240 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 300 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 360 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 420 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 480 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 660 gccccccctc gaggtcgacg gtatcgatat taattaacat gcatgtcccg cgttcgtgaa 720 attctctgca tcagcggagt gatcaggaat catcgtctca gcgggatgac gttgcggagc 780 aggccggctc gcggtgggca gtcagatgcc gaaggcgtaa ctcaggacgg cttgcgctca 840 tcgcagaaca ggggtggtgc ctgcattggg tgcggttggt gatcctggtt ggaccggtgg 900 agatgcgcgc gcacgaaggg gatgtgtcag aaacattttg tttgttctct gtgaactttt 960 agatgtgtta aaggcggcga atattagcag agagtcctcc ttgttggatt ctctcttgaa 1020 tttcgccctt tccttctctt tgcgagtctc gtagagaaca agcactcgtt cgccgtccct 1080 gacgacgcaa cccgcgcaga agacatccac caaacggtgt tacacaatca ccttgtgtga 1140 agttcttgcg gaaaactact cgttggcatt ttttcttgaa ttcccttttt cgacaatggg 1200 ttctcatcat catcatcatc atggtatggc tagcatgact ggtggacagc aaatgggtcg 1260 ggatctgtac gacgatgacg ataaggatct cgccaccatg gtcgactcat cacgtcgtaa 1320 gtggaataag acaggtcacg cagtcagagc tataggtcgg ctgagctcac tcgagaacgt 1380 ctatatcaag gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa 1440 catcgaggac ggcggcgtgc agctcgccta ccactaccag cagaacaccc ccatcggcga 1500 cggccccgtg ctgctgcccg acaaccacta cctgagcgtg cagtccaaac tttcgaaaga 1560 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac 1620 tctcggcatg gacgagctgt acaagggcgg taccggaggg agcatggtga gcaagggcga 1680 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca 1740 caagttcagc gtgtccggcg agggtgaggg cgatgccacc tacggcaagc tgaccctgaa 1800 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac 1860 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa 1920 gtccgccatg cccgaaggct acatccagga gcgcaccatc ttcttcaagg acgacggcaa 1980 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct 2040 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacct 2100 gccggaccaa ctgactgaag agcagatcgc agaatttaaa gaggaattct ccctatttga 2160 caaggacggg gatgggacaa taacaaccaa ggagctgggg acggtgatgc ggtccctggg 2220 gcagaacccc acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga 2280 cggcacaatc gacttccctg agttcctgac aatgatggca agaaaaatga aatacaggga 2340 cacggaagaa gaaattagag aagcgttcgg tgtgtttgat aaggatggca atggctacat 2400 cagtgcagca gagcttcgcc acgtgatgac aaaccttgga gagaagttaa cagatgaaga 2460 ggttgatgaa atgatcaggg aagcagacat cgatggggat ggtcaggtaa actacgaaga 2520 gtttgtacaa atgatgacag cgaagtaaac agaagctgcc cgtctctcgt tttcctctct 2580 tttcggaggg atcagggaga gtgcctcggg tcggagagag ctgacgaggg ggtgccagag 2640 acccctgtgt cctttatcga agaaaaggga tgactcttca tgtggcattt cacacagtct 2700 cacctcgcct tgttttcttt ttgtcaatca gaacgaaagc gagttgcggg tgacgcagat 2760 gtgcgtgtat ccactcgnga atgcgttatc gttctgtatg ccgctagagt gctggactgt 2820 tgctgtctgc ccacgacagc agacaacttt ccttctatgc acttgcagga tggtgcagcg 2880 caaacgacgg agagaaagga gcaccctctc agtttcccta cgatgtgctg tcagtttcga 2940 ctcttcaccg cgaacgattg gcgatacgtc tctgttgact tgttaggctc cgaccacgaa 3000 gctcccttaa ctagataagc cgcgacacct aagtgtacac catttgcaga tcgataatct 3060 gcgaccgctg aatccgtcca gatcagtaaa accgcaccac ctaagtgtaa accttgttta 3120 ggtcgataaa atgctaccaa cccccaccca caatcgagcc ttgagcgttt ctgcgcacgc 3180 gttggcctac gtgacttgct gatgcctgcc tctggccatt catgccagtc agtgcgcata 3240 aaaatgtgga cacagtcggt tgacaagtgt tctggcaggc tacagtgaca cgcggccgca 3300 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat 3360 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 3420 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 3480 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 3540 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 3600 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 3660 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 3720 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 3780 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 3840 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 3900 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 3960 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 4020 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 4080 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 4140 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 4200 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 4260 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 4320 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 4380 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 4440 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 4500 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 4560 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 4620 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 4680 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 4740 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 4800 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 4860 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 4920 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 4980 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 5040 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 5100 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 5160 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 5220 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 5280 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 5340 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 5400 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 5460 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac 5505 // ID MT482301; SV 1; linear; other DNA; STD; SYN; 1122 BP. XX AC MT482301; XX DT 20-JUL-2020 (Rel. 144, Created) DT 20-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct PelB/keratinase gene, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1122 RA Rahimnahal S., Karkhane A.A., Meimandipour A., Fayazi J., Shamsara M., RA Nassiri M.T.B., Tarrahimofrad H.; RT ; RL Submitted (15-MAY-2020) to the INSDC. RL Animal Science, Ramin Agriculture and Natural Resources University of RL Khuzestan, Khuzestan Agricultural Sciences and Natural Resources RL University, Mollasani, Ahvaz, Khuzestan 6341773637, Iran XX DR MD5; 30697cb4d1a91b9e4b1f762f780499c6. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1122 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Bacillus licheniformis LRKr1" FT /db_xref="taxon:32630" FT CDS 4..>1122 FT /codon_start=1 FT /transl_table=11 FT /product="PelB/keratinase" FT /note="codon-optimized for expression in Escherichia coli" FT /protein_id="QLE11229.1" FT /translation="MKYLLPTAAAGLLLLAAQPAMAQPAKNVEKDYIVGFKSGVKTASV FT KKDIIKESGGKVDKQFRIINAAKAKLDKEALKEVKNDPDVAYVEEDHVAHALAQTVPYG FT IPLIKADKVQAQGFKGANVKVAVLDTGIQASHPDLNVVGGASFVAGEAYNTDGNGHGTH FT VAGTVAALDNTTGVLGVAPSVSLYAVKVLNSSGSGSYSGIVSGIEWATTNGMDVINMSL FT GGASGSTAMKQAVDNAYARGVVVVAAAGNSGSSGNTNTIGYPAKYDSVIAVGAVDSNSN FT RASFSSVGAELEVMAPGAGVYSTYPTNTYATLNGTSMASPHVAGAAALILSKHPNLSAS FT QVRNRLSSTATYLGSSFYYGKGLINVEGAAQLE" XX SQ Sequence 1122 BP; 291 A; 270 C; 317 G; 244 T; 0 other; catatgaaat acctgctgcc gaccgctgct gctggtctgc tgctcctcgc tgcccagccg 60 gccatggccc agccggcgaa aaacgtcgaa aaggattaca ttgtcggatt taaatccggc 120 gtgaaaaccg cctcagttaa gaaagatata atcaaggaat ctggcggaaa agtagataag 180 caattcagaa taattaatgc agctaaagcc aagttagaca aagaagcgct gaaagaggtt 240 aagaatgacc ccgacgtggc atacgtcgag gaggaccatg tagcgcacgc cttagcccag 300 acggtaccct acggtattcc cttgataaag gccgacaagg tacaggcgca ggggtttaag 360 ggtgcgaatg tgaaggttgc cgtacttgac acagggattc aagcctcgca tcccgacctt 420 aatgttgtcg ggggcgctag ttttgtagca ggagaagcat ataataccga cggcaacggt 480 cacggtacac atgtggcggg caccgtggca gccttagata acactaccgg tgtgctgggt 540 gtcgcaccga gtgttagctt gtacgcggtg aaagtattga actcctctgg aagtggatcc 600 tactctggga tagtcagcgg tatcgagtgg gctaccacaa acggcatgga cgtaataaat 660 atgagtcttg gtggggcgtc aggctcgaca gccatgaagc aggccgttga taatgcttat 720 gcacgggggg tcgtggttgt ggcagccgct ggcaacagtg gcagctcggg gaacacgaac 780 actatcggct acccagccaa atatgattct gtcatcgcag taggtgccgt cgactccaac 840 tctaatcgtg cttcgttcag ctcagttgga gccgaacttg aggtgatggc acctggtgct 900 ggagtgtact cgacataccc aactaatact tatgcaacct tgaacggcac atcaatggca 960 tcaccccacg tagctggcgc agcagcgctt attttaagta agcatcccaa tctgagtgcc 1020 tcgcaggttc ggaatcgcct gagtagcacg gcaacttacc tgggaagttc attttattac 1080 ggaaagggtt taatcaacgt ggagggagct gctcaactcg ag 1122 // ID MT591673; SV 1; linear; other DNA; STD; SYN; 7895 BP. XX AC MT591673; XX DT 20-JUL-2020 (Rel. 144, Created) DT 20-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning Vector pBMNZ-mCD8aLck-mCherry, complete sequence. XX KW . XX OS Cloning Vector pBMNZ-mCD8aLck-mCherry OC other sequences; artificial sequences; vectors. XX RN [1] RC Publication Status: Available-Online prior to print RP 1-7895 RA Wei Q., Brzostek J., Sankaran S., Casas J., Hew L.S., Yap J., Zhao X., RA Wojciech L., Gascoigne N.R.J.; RT "Lck bound to coreceptor is less active than free Lck"; RL Proc. Natl. Acad. Sci. U.S.A.:(2020). XX RN [2] RP 1-7895 RA Wei Q., Brzostek J., Casas J.; RT ; RL Submitted (10-JUN-2020) to the INSDC. RL Microbiology and Immunology, National University of Singapore, Block MD4, 5 RL Science Drive 2, NUS, Singapore 117545, Singapore XX DR MD5; 6348959eacb111eb5022f2ceb3dd0d56. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..7895 FT /organism="Cloning Vector pBMNZ-mCD8aLck-mCherry" FT /mol_type="other DNA" FT /db_xref="taxon:2751469" FT CDS 2331..5228 FT /codon_start=1 FT /transl_table=11 FT /product="CD8aLck-mCherry" FT /note="mouse CD8a, mouse Lck and mCherry fusion protein. FT CD8a and Lck are fused at the Zinc Clasp; Lck and mCherry FT are fused via a flexable linker. Protein is coded by FT synthetic DNA." FT /protein_id="QLF95854.1" FT /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL FT GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA FT MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP FT VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHRS FT RKRVIVPLDSKISLPIRNGSEVRDPLVTYEGSLPPASPLQDNLVIALHSYEPSHDGDLG FT FEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQ FT LLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITF FT PGLHDLVRHYTNASDGLCTKLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGE FT VWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHPRLVRLYAVVTQEPIYIITE FT YMENGSLVDFLKTPSGIKLNVNKLLDMAAQIAEGMAFIEEQNYIHRDLRAANILVSDTL FT SCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVT FT HGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYHLMMLCWKERPEDRPTFDYLRSVL FT DDFFTATEGQYQPQPGGGGSGGGGSGGGGSGDPRVMVSKGEEDNMAIIKEFMRFKVHME FT GSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPAD FT IPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQ FT KKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVN FT IKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK" XX SQ Sequence 7895 BP; 1893 A; 2174 C; 2019 G; 1809 T; 0 other; ggccctttcg tcttcaagaa cagctttgct cttaggagtt tcctaataca tcccaaactc 60 aaatatataa agcatttgac ttgttctatg ccctaggggg cggggggaag ctaagccagc 120 tttttttaac atttaaaatg ttaattccat tttaaatgca cagatgtttt tatttcataa 180 gggtttcaat gtgcatgaat gctgcaatat tcctgttacc aaagctagta taaataaaaa 240 tagataaacg tggaaattac ttagagtttc tgtcattaac gtttccttcc tcagttgaca 300 acataaatgc gctgctgaga agccagtttg catctgtcag gatcaatttc ccattatgcc 360 agtcatatta attactagtc aattagttga tttttatttt tgacatatac atgtgaaaga 420 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa 480 tacataactg agaatagaaa agttcagatc aaggtcagga acagatggaa cagctgaata 540 tgggccaaag cggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga 600 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc 660 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca 720 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac 780 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag 840 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt 900 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg 960 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg 1020 gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag ctggccagca 1080 acttatctgt gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg 1140 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttcgga 1200 acacccggcc gcaaccctgg gagacgtccc agggacttcg ggggccgttt ttgtggcccg 1260 acctgagtcc aaaaatcccg atcgttttgg actctttggt gcacccccct tagaggaggg 1320 atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt 1380 gctttcggtt tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg 1440 ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg gccagactgt 1500 taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa 1560 ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac 1620 ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa 1680 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac 1740 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc 1800 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc 1860 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata 1920 tgagatctta tatggggcac ccccgcccct tgtaaacttc cctgaccctg acatgacaag 1980 agttactaac agcccctctc tccaagctca cttacaggct ctctacttag tccagcacga 2040 agtctggaga cctctggcgg cagcctacca agaacaactg gaccgaccgg tggtacctca 2100 cccttaccga gtcggcgaca cagtgtgggt ccgccgacac cagactaaga acctagaacc 2160 tcgctggaaa ggaccttaca cagtcctgct gaccaccccc accgccctca aagtagacgg 2220 catcgcagct tggatacacg ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc 2280 ctctagactg ccggatccca gtgtggtggt acgggaattc aagcttgacc atggcctcac 2340 cgttgacccg ctttctgtcg ctgaacctgc tgctgctggg tgagtcgatt atcctgggga 2400 gtggagaagc taagccacag gcacccgaac tccgaatctt tccaaagaaa atggacgccg 2460 aacttggtca gaaggtggac ctggtatgtg aagtgttggg gtccgtttcg caaggatgct 2520 cttggctctt ccagaactcc agctccaaac tcccccagcc caccttcgtt gtctatatgg 2580 cttcatccca caacaagata acgtgggacg agaagctgaa ttcgtcgaaa ctgttttctg 2640 ccatgaggga cacgaataat aagtacgttc tcaccctgaa caagttcagc aaggaaaacg 2700 aaggctacta tttctgctca gtcatcagca actcggtgat gtacttcagt tctgtcgtgc 2760 cagtccttca gaaagtgaac tctactacta ccaagccagt gctgcgaact ccctcacctg 2820 tgcaccctac cgggacatct cagccccaga gaccagaaga ttgtcggccc cgtggctcag 2880 tgaaggggac cggattggac ttcgcctgtg atatttacat ctgggcaccc ttggccggaa 2940 tctgcgtggc ccttctgctg tccttgatca tcactctcat ctgctaccac aggagccgaa 3000 agcgtgttat agtcccactg gacagcaaga tctcgctgcc catccggaat ggctctgaag 3060 tgcgggaccc actggtcacc tatgagggat ctctcccacc agcatccccg ctgcaagaca 3120 acctggttat cgccctgcac agttatgagc cctcccatga tggagacttg ggctttgaga 3180 agggtgaaca gctccgaatc ctggagcaga gcggtgagtg gtggaaggct cagtccctga 3240 cgactggcca agaaggcttc attcccttca acttcgtggc gaaagcaaac agcctggagc 3300 ctgaaccttg gttcttcaag aatctgagcc gtaaggacgc cgagcggcag cttttggcgc 3360 ccgggaacac gcatggatcc ttcctgatcc gggaaagcga aagcactgcg gggtcctttt 3420 ccctgtcggt cagagacttc gaccagaacc agggagaagt ggtgaaacat tacaagatcc 3480 gtaacctaga caacggtggc ttctacatct cccctcgtat cacttttccc ggattgcacg 3540 atctagtccg ccattacacc aacgcctctg atgggctgtg cacaaagttg agccgtcctt 3600 gccagaccca gaagccccag aaaccatggt gggaggacga atgggaagtt cccagggaaa 3660 cactgaagtt ggtggagcgg ctgggagctg gccagttcgg ggaagtgtgg atggggtact 3720 acaacggaca cacgaaggtg gcggtgaaga gtctgaaaca agggagcatg tcccccgacg 3780 ccttcctggc tgaggctaac ctcatgaagc agctgcagca cccgcggcta gtccggcttt 3840 atgcagtggt cacccaggaa cccatctaca tcatcacgga atacatggag aacgggagcc 3900 tagtagattt tctcaagact ccctcgggca tcaagttgaa tgtcaacaaa cttttggaca 3960 tggcagccca gattgcagag ggcatggcgt tcatcgaaga acagaattac atccatcggg 4020 acctgcgcgc cgccaacatc ctggtgtctg acacgctgag ctgcaagatt gcagactttg 4080 gcctggcgcg cctcattgag gacaatgagt acacggcccg ggagggggcc aaatttccca 4140 ttaagtggac agcaccagaa gccattaact atgggacctt caccatcaag tcagacgtgt 4200 ggtccttcgg gatcttgctt acagagatcg tcacccacgg tcgaatccct tacccaggaa 4260 tgaccaaccc tgaagtcatt cagaacctgg agagaggcta ccgcatggtg agacctgaca 4320 actgtccgga agagctgtac cacctcatga tgctgtgctg gaaggagcgc ccagaggacc 4380 ggcccacgtt tgactacctt cggagtgttc tggatgactt cttcacagcc acagagggcc 4440 agtaccagcc ccagcctggt ggaggcggta gcggtggagg tgggagcggt ggaggtggca 4500 gcggggatcc tcgagtcatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg 4560 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg 4620 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca 4680 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca 4740 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg 4800 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg 4860 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc 4920 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga 4980 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg 5040 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc 5100 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca 5160 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt 5220 acaagtaagc ggccgccagc acagtggtcg acgataaaat aaaagatttt atttagtctc 5280 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg 5340 ccattttgca aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca 5400 ggaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag cagttcctgc 5460 cccggctcag ggccaagaac agatggaaca gctgaatatg ggccaaacag gatatctgtg 5520 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtccagc 5580 cctcagcagt ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga 5640 ccctgtgcct tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct 5700 gctccccgag ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt 5760 gactgagtcg cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg 5820 tggtctcgct gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt 5880 ctttcattca catgcagcat gtatcaaaat taatttggtt ttttttctta agtatttaca 5940 ttaaatggcc atagttgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6000 ttggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 6060 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 6120 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 6180 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 6240 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 6300 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 6360 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 6420 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 6480 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 6540 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 6600 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 6660 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 6720 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 6780 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 6840 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 6900 aagtttgcgc aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 6960 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 7020 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 7080 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 7140 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 7200 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 7260 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 7320 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 7380 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 7440 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 7500 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 7560 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 7620 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 7680 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 7740 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 7800 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 7860 catgacatta acctataaaa ataggcgtat cacga 7895 // ID MT591674; SV 1; linear; other DNA; STD; SYN; 7910 BP. XX AC MT591674; XX DT 20-JUL-2020 (Rel. 144, Created) DT 20-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning Vector pBMNZ-mCD8aLck-eGFP, complete sequence. XX KW . XX OS Cloning Vector pBMNZ-mCD8aLck-eGFP OC other sequences; artificial sequences; vectors. XX RN [1] RC Publication Status: Available-Online prior to print RP 1-7910 RA Wei Q., Brzostek J., Sankaran S., Casas J., Hew L.S., Yap J., Zhao X., RA Wojciech L., Gascoigne N.R.J.; RT "Lck bound to coreceptor is less active than free Lck"; RL Proc. Natl. Acad. Sci. U.S.A.:(2020). XX RN [2] RP 1-7910 RA Wei Q., Brzostek J., Casas J.; RT ; RL Submitted (10-JUN-2020) to the INSDC. RL Microbiology and Immunology, National University of Singapore, Block MD4, 5 RL Science Drive 2, NUS, Singapore 117545, Singapore XX DR MD5; 365040c79156e99449d01c00a0a7f8ba. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..7910 FT /organism="Cloning Vector pBMNZ-mCD8aLck-eGFP" FT /mol_type="other DNA" FT /db_xref="taxon:2751468" FT CDS 2331..5243 FT /codon_start=1 FT /transl_table=11 FT /product="CD8aLck-eGFP" FT /note="mouse CD8alpha, mouse Lck and eGFP fusion protein; FT CD8alpha and Lck are fused at the Zinc clasp; Lck and eGFP FT are fused via a flexible linker" FT /protein_id="QLF95855.1" FT /translation="MASPLTRFLSLNLLLLGESIILGSGEAKPQAPELRIFPKKMDAEL FT GQKVDLVCEVLGSVSQGCSWLFQNSSSKLPQPTFVVYMASSHNKITWDEKLNSSKLFSA FT MRDTNNKYVLTLNKFSKENEGYYFCSVISNSVMYFSSVVPVLQKVNSTTTKPVLRTPSP FT VHPTGTSQPQRPEDCRPRGSVKGTGLDFACDIYIWAPLAGICVALLLSLIITLICYHRS FT RKRVIVPLDSKISLPIRNGSEVRDPLVTYEGSLPPASPLQDNLVIALHSYEPSHDGDLG FT FEKGEQLRILEQSGEWWKAQSLTTGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQ FT LLAPGNTHGSFLIRESESTAGSFSLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITF FT PGLHDLVRHYTNASDGLCTKLSRPCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGE FT VWMGYYNGHTKVAVKSLKQGSMSPDAFLAEANLMKQLQHPRLVRLYAVVTQEPIYIITE FT YMENGSLVDFLKTPSGIKLNVNKLLDMAAQIAEGMAFIEEQNYIHRDLRAANILVSDTL FT SCKIADFGLARLIEDNEYTAREGAKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVT FT HGRIPYPGMTNPEVIQNLERGYRMVRPDNCPEELYHLMMLCWKERPEDRPTFDYLRSVL FT DDFFTATEGQYQPQPGGGGSGGGGSGGGGSGDPRVATMVSKGEELFTGVVPILVELDGD FT VNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQ FT HDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGH FT KLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDN FT HYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" XX SQ Sequence 7910 BP; 1903 A; 2193 C; 2003 G; 1811 T; 0 other; ggccctttcg tcttcaagaa cagctttgct cttaggagtt tcctaataca tcccaaactc 60 aaatatataa agcatttgac ttgttctatg ccctaggggg cggggggaag ctaagccagc 120 tttttttaac atttaaaatg ttaattccat tttaaatgca cagatgtttt tatttcataa 180 gggtttcaat gtgcatgaat gctgcaatat tcctgttacc aaagctagta taaataaaaa 240 tagataaacg tggaaattac ttagagtttc tgtcattaac gtttccttcc tcagttgaca 300 acataaatgc gctgctgaga agccagtttg catctgtcag gatcaatttc ccattatgcc 360 agtcatatta attactagtc aattagttga tttttatttt tgacatatac atgtgaaaga 420 ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg catggaaaaa 480 tacataactg agaatagaaa agttcagatc aaggtcagga acagatggaa cagctgaata 540 tgggccaaag cggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga 600 tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc 660 agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc tagagaacca 720 tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac 780 caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag 840 cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc gggtacccgt 900 gtatccaata aaccctcttg cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg 960 gtctcctctg agtgattgac tacccgtcag cgggggtctt tcatttgggg gctcgtccgg 1020 gatcgggaga cccctgccca gggaccaccg acccaccacc gggaggtaag ctggccagca 1080 acttatctgt gtctgtccga ttgtctagtg tctatgactg attttatgcg cctgcgtcgg 1140 tactagttag ctaactagct ctgtatctgg cggacccgtg gtggaactga cgagttcgga 1200 acacccggcc gcaaccctgg gagacgtccc agggacttcg ggggccgttt ttgtggcccg 1260 acctgagtcc aaaaatcccg atcgttttgg actctttggt gcacccccct tagaggaggg 1320 atatgtggtt ctggtaggag acgagaacct aaaacagttc ccgcctccgt ctgaattttt 1380 gctttcggtt tgggaccgaa gccgcgccgc gcgtcttgtc tgctgcagca tcgttctgtg 1440 ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat atgggcccgg gccagactgt 1500 taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa 1560 ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac 1620 ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa 1680 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac 1740 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc 1800 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc 1860 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccccca tatggccata 1920 tgagatctta tatggggcac ccccgcccct tgtaaacttc cctgaccctg acatgacaag 1980 agttactaac agcccctctc tccaagctca cttacaggct ctctacttag tccagcacga 2040 agtctggaga cctctggcgg cagcctacca agaacaactg gaccgaccgg tggtacctca 2100 cccttaccga gtcggcgaca cagtgtgggt ccgccgacac cagactaaga acctagaacc 2160 tcgctggaaa ggaccttaca cagtcctgct gaccaccccc accgccctca aagtagacgg 2220 catcgcagct tggatacacg ccgcccacgt gaaggctgcc gaccccgggg gtggaccatc 2280 ctctagactg ccggatccca gtgtggtggt acgggaattc aagcttgacc atggcctcac 2340 cgttgacccg ctttctgtcg ctgaacctgc tgctgctggg tgagtcgatt atcctgggga 2400 gtggagaagc taagccacag gcacccgaac tccgaatctt tccaaagaaa atggacgccg 2460 aacttggtca gaaggtggac ctggtatgtg aagtgttggg gtccgtttcg caaggatgct 2520 cttggctctt ccagaactcc agctccaaac tcccccagcc caccttcgtt gtctatatgg 2580 cttcatccca caacaagata acgtgggacg agaagctgaa ttcgtcgaaa ctgttttctg 2640 ccatgaggga cacgaataat aagtacgttc tcaccctgaa caagttcagc aaggaaaacg 2700 aaggctacta tttctgctca gtcatcagca actcggtgat gtacttcagt tctgtcgtgc 2760 cagtccttca gaaagtgaac tctactacta ccaagccagt gctgcgaact ccctcacctg 2820 tgcaccctac cgggacatct cagccccaga gaccagaaga ttgtcggccc cgtggctcag 2880 tgaaggggac cggattggac ttcgcctgtg atatttacat ctgggcaccc ttggccggaa 2940 tctgcgtggc ccttctgctg tccttgatca tcactctcat ctgctaccac aggagccgaa 3000 agcgtgttat agtcccactg gacagcaaga tctcgctgcc catccggaat ggctctgaag 3060 tgcgggaccc actggtcacc tatgagggat ctctcccacc agcatccccg ctgcaagaca 3120 acctggttat cgccctgcac agttatgagc cctcccatga tggagacttg ggctttgaga 3180 agggtgaaca gctccgaatc ctggagcaga gcggtgagtg gtggaaggct cagtccctga 3240 cgactggcca agaaggcttc attcccttca acttcgtggc gaaagcaaac agcctggagc 3300 ctgaaccttg gttcttcaag aatctgagcc gtaaggacgc cgagcggcag cttttggcgc 3360 ccgggaacac gcatggatcc ttcctgatcc gggaaagcga aagcactgcg gggtcctttt 3420 ccctgtcggt cagagacttc gaccagaacc agggagaagt ggtgaaacat tacaagatcc 3480 gtaacctaga caacggtggc ttctacatct cccctcgtat cacttttccc ggattgcacg 3540 atctagtccg ccattacacc aacgcctctg atgggctgtg cacaaagttg agccgtcctt 3600 gccagaccca gaagccccag aaaccatggt gggaggacga atgggaagtt cccagggaaa 3660 cactgaagtt ggtggagcgg ctgggagctg gccagttcgg ggaagtgtgg atggggtact 3720 acaacggaca cacgaaggtg gcggtgaaga gtctgaaaca agggagcatg tcccccgacg 3780 ccttcctggc tgaggctaac ctcatgaagc agctgcagca cccgcggcta gtccggcttt 3840 atgcagtggt cacccaggaa cccatctaca tcatcacgga atacatggag aacgggagcc 3900 tagtagattt tctcaagact ccctcgggca tcaagttgaa tgtcaacaaa cttttggaca 3960 tggcagccca gattgcagag ggcatggcgt tcatcgaaga acagaattac atccatcggg 4020 acctgcgcgc cgccaacatc ctggtgtctg acacgctgag ctgcaagatt gcagactttg 4080 gcctggcgcg cctcattgag gacaatgagt acacggcccg ggagggggcc aaatttccca 4140 ttaagtggac agcaccagaa gccattaact atgggacctt caccatcaag tcagacgtgt 4200 ggtccttcgg gatcttgctt acagagatcg tcacccacgg tcgaatccct tacccaggaa 4260 tgaccaaccc tgaagtcatt cagaacctgg agagaggcta ccgcatggtg agacctgaca 4320 actgtccgga agagctgtac cacctcatga tgctgtgctg gaaggagcgc ccagaggacc 4380 ggcccacgtt tgactacctt cggagtgttc tggatgactt cttcacagcc acagagggcc 4440 agtaccagcc ccagcctggt ggaggcggta gcggtggagg tgggagcggt ggaggtggca 4500 gcggggatcc tcgagtcgcc accatggtga gcaagggcga ggagctgttc accggggtgg 4560 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 4620 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 4680 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 4740 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 4800 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 4860 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 4920 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 4980 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 5040 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 5100 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 5160 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 5220 gcatggacga gctgtacaag taagcggccg ccagcacagt ggtcgacgat aaaataaaag 5280 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag 5340 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag 5400 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg 5460 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca 5520 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc 5580 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc 5640 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc 5700 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc 5760 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca 5820 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta 5880 cccgtcagcg ggggtctttc attcacatgc agcatgtatc aaaattaatt tggttttttt 5940 tcttaagtat ttacattaaa tggccatagt tgcattaatg aatcggccaa cgcgcgggga 6000 gaggcggttt gcgtattggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6060 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 6120 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 6180 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 6240 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 6300 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 6360 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 6420 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 6480 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 6540 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 6600 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 6660 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 6720 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 6780 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 6840 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 6900 tttaaattaa aaatgaagtt tgcgcaaatc aatctaaagt atatatgagt aaacttggtc 6960 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 7020 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 7080 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 7140 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7200 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 7260 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 7320 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 7380 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 7440 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 7500 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 7560 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 7620 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 7680 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 7740 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 7800 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 7860 tcagggttat tgtctcatga cattaaccta taaaaatagg cgtatcacga 7910 // ID MT591675; SV 1; linear; other DNA; STD; SYN; 7296 BP. XX AC MT591675; XX DT 20-JUL-2020 (Rel. 144, Created) DT 20-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning Vector pBMNZ-mLck-mCherry, complete sequence. XX KW . XX OS Cloning Vector pBMNZ-mLck-mCherry OC other sequences; artificial sequences; vectors. XX RN [1] RC Publication Status: Available-Online prior to print RP 1-7296 RA Wei Q., Brzostek J., Sankaran S., Casas J., Hew L.S., Yap J., Zhao X., RA Wojciech L., Gascoigne N.R.J.; RT "Lck bound to coreceptor is less active than free Lck"; RL Proc. Natl. Acad. Sci. U.S.A.:(2020). XX RN [2] RP 1-7296 RA Wei Q., Brzostek J., Casas J.; RT ; RL Submitted (10-JUN-2020) to the INSDC. RL Microbiology and Immunology, National University of Singapore, Block MD4, 5 RL Science Drive 2, NUS, Singapore 117545, Singapore XX DR MD5; 9115f29b911ae60bd0da88b6ad185736. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..7296 FT /organism="Cloning Vector pBMNZ-mLck-mCherry" FT /mol_type="other DNA" FT /db_xref="taxon:2751470" FT CDS 2332..4629 FT /codon_start=1 FT /transl_table=11 FT /product="Lck-mCherry" FT /note="mouse Lck fused with mCherry via a flexable linker" FT /protein_id="QLF95856.1" FT /translation="MGCVCSSNPEDDWMENIDVCENCHYPIVPLDSKISLPIRNGSEVR FT DPLVTYEGSLPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLT FT TGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSF FT SLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHDLVRHYTNASDGLCTKLSR FT PCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMS FT PDAFLAEANLMKQLQHPRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLNVNK FT LLDMAAQIAEGMAFIEEQNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREG FT AKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYR FT MVRPDNCPEELYHLMMLCWKERPEDRPTFDYLRSVLDDFFTATEGQYQPQPGGGGSGGG FT GSGGGGSGDPPVMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ FT TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED FT GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE FT IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG FT RHSTGGMDELYK" XX SQ Sequence 7296 BP; 1758 A; 2001 C; 1874 G; 1663 T; 0 other; aggccctttc gtcttcaaga acagctttgc tcttaggagt ttcctaatac atcccaaact 60 caaatatata aagcatttga cttgttctat gccctagggg gcggggggaa gctaagccag 120 ctttttttaa catttaaaat gttaattcca ttttaaatgc acagatgttt ttatttcata 180 agggtttcaa tgtgcatgaa tgctgcaata ttcctgttac caaagctagt ataaataaaa 240 atagataaac gtggaaatta cttagagttt ctgtcattaa cgtttccttc ctcagttgac 300 aacataaatg cgctgctgag aagccagttt gcatctgtca ggatcaattt cccattatgc 360 cagtcatatt aattactagt caattagttg atttttattt ttgacatata catgtgaaag 420 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa 480 atacataact gagaatagaa aagttcagat caaggtcagg aacagatgga acagctgaat 540 atgggccaaa gcggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag 600 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct 660 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc 720 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa 780 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga 840 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg 900 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag 960 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg 1020 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggccagc 1080 aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg 1140 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg 1200 aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc 1260 gacctgagtc caaaaatccc gatcgttttg gactctttgg tgcacccccc ttagaggagg 1320 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt 1380 tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt 1440 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg ggccagactg 1500 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca 1560 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa 1620 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta 1680 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga 1740 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc 1800 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc 1860 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat 1920 atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa 1980 gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg 2040 aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc 2100 acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac 2160 ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg 2220 gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat 2280 cctctagact gccggatccc agtgtggtgg tacgggaatt caagcttgac catgggctgt 2340 gtctgcagct caaaccctga agatgactgg atggagaaca ttgacgtgtg tgaaaactgc 2400 cactatccca tagtcccact ggacagcaag atctcgctgc ccatccggaa tggctctgaa 2460 gtgcgggacc cactggtcac ctatgaggga tctctcccac cagcatcccc gctgcaagac 2520 aacctggtta tcgccctgca cagttatgag ccctcccatg atggagactt gggctttgag 2580 aagggtgaac agctccgaat cctggagcag agcggtgagt ggtggaaggc tcagtccctg 2640 acgactggcc aagaaggctt cattcccttc aacttcgtgg cgaaagcaaa cagcctggag 2700 cctgaacctt ggttcttcaa gaatctgagc cgtaaggacg ccgagcggca gcttttggcg 2760 cccgggaaca cgcatggatc cttcctgatc cgggaaagcg aaagcactgc ggggtccttt 2820 tccctgtcgg tcagagactt cgaccagaac cagggagaag tggtgaaaca ttacaagatc 2880 cgtaacctag acaacggtgg cttctacatc tcccctcgta tcacttttcc cggattgcac 2940 gatctagtcc gccattacac caacgcctct gatgggctgt gcacaaagtt gagccgtcct 3000 tgccagaccc agaagcccca gaaaccatgg tgggaggacg aatgggaagt tcccagggaa 3060 acactgaagt tggtggagcg gctgggagct ggccagttcg gggaagtgtg gatggggtac 3120 tacaacggac acacgaaggt ggcggtgaag agtctgaaac aagggagcat gtcccccgac 3180 gccttcctgg ctgaggctaa cctcatgaag cagctgcagc acccgcggct agtccggctt 3240 tatgcagtgg tcacccagga acccatctac atcatcacgg aatacatgga gaacgggagc 3300 ctagtagatt ttctcaagac tccctcgggc atcaagttga atgtcaacaa acttttggac 3360 atggcagccc agattgcaga gggcatggcg ttcatcgaag aacagaatta catccatcgg 3420 gacctgcgcg ccgccaacat cctggtgtct gacacgctga gctgcaagat tgcagacttt 3480 ggcctggcgc gcctcattga ggacaatgag tacacggccc gggagggggc caaatttccc 3540 attaagtgga cagcaccaga agccattaac tatgggacct tcaccatcaa gtcagacgtg 3600 tggtccttcg ggatcttgct tacagagatc gtcacccacg gtcgaatccc ttacccagga 3660 atgaccaacc ctgaagtcat tcagaacctg gagagaggct accgcatggt gagacctgac 3720 aactgtccgg aagagctgta ccacctcatg atgctgtgct ggaaggagcg cccagaggac 3780 cggcccacgt ttgactacct tcggagtgtt ctggatgact tcttcacagc cacagagggc 3840 cagtaccagc cccagcctgg tggaggcggt agcggtggag gtgggagcgg tggaggtggc 3900 agcggggatc caccggtcat ggtgagcaag ggcgaggagg ataacatggc catcatcaag 3960 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc 4020 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 4080 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc 4140 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag 4200 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag 4260 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc 4320 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg 4380 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac 4440 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg 4500 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc 4560 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg 4620 tacaagtaaa gcggccgcca gcacagtggt cgacgataaa ataaaagatt ttatttagtc 4680 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa 4740 cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt 4800 caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct 4860 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg 4920 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca 4980 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat 5040 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt 5100 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga 5160 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact 5220 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg 5280 gtctttcatt cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta 5340 cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 5400 tattggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 5460 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 5520 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 5580 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 5640 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 5700 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 5760 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 5820 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 5880 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 5940 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 6000 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 6060 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 6120 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 6180 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 6240 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 6300 tgaagtttgc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 6360 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 6420 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 6480 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 6540 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 6600 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 6660 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 6720 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 6780 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 6840 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 6900 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 6960 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 7020 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 7080 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 7140 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 7200 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 7260 ctcatgacat taacctataa aaataggcgt atcacg 7296 // ID MT591676; SV 1; linear; other DNA; STD; SYN; 7296 BP. XX AC MT591676; XX DT 20-JUL-2020 (Rel. 144, Created) DT 20-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning Vector pBMNZ-mLckC20.23A-mCherry, complete sequence. XX KW . XX OS Cloning Vector pBMNZ-mLckC20.23A-mCherry OC other sequences; artificial sequences; vectors. XX RN [1] RC Publication Status: Available-Online prior to print RP 1-7296 RA Wei Q., Brzostek J., Sankaran S., Casas J., Hew L.S., Yap J., Zhao X., RA Wojciech L., Gascoigne N.R.J.; RT "Lck bound to coreceptor is less active than free Lck"; RL Proc. Natl. Acad. Sci. U.S.A.:(2020). XX RN [2] RP 1-7296 RA Wei Q., Brzostek J., Casas J.; RT ; RL Submitted (10-JUN-2020) to the INSDC. RL Microbiology and Immunology, National University of Singapore, Block MD4, 5 RL Science Drive 2, NUS, Singapore 117545, Singapore XX DR MD5; ee8a5619ef017e81334993d023ed0aa7. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..7296 FT /organism="Cloning Vector pBMNZ-mLckC20.23A-mCherry" FT /mol_type="other DNA" FT /db_xref="taxon:2751471" FT CDS 2332..4629 FT /codon_start=1 FT /transl_table=11 FT /product="Lck(C20.23A)-mCherry" FT /note="mouse Lck with C20.23A mutant fused with mCherry via FT a flexable linker" FT /protein_id="QLF95857.1" FT /translation="MGCVCSSNPEDDWMENIDVAENAHYPIVPLDSKISLPIRNGSEVR FT DPLVTYEGSLPPASPLQDNLVIALHSYEPSHDGDLGFEKGEQLRILEQSGEWWKAQSLT FT TGQEGFIPFNFVAKANSLEPEPWFFKNLSRKDAERQLLAPGNTHGSFLIRESESTAGSF FT SLSVRDFDQNQGEVVKHYKIRNLDNGGFYISPRITFPGLHDLVRHYTNASDGLCTKLSR FT PCQTQKPQKPWWEDEWEVPRETLKLVERLGAGQFGEVWMGYYNGHTKVAVKSLKQGSMS FT PDAFLAEANLMKQLQHPRLVRLYAVVTQEPIYIITEYMENGSLVDFLKTPSGIKLNVNK FT LLDMAAQIAEGMAFIEEQNYIHRDLRAANILVSDTLSCKIADFGLARLIEDNEYTAREG FT AKFPIKWTAPEAINYGTFTIKSDVWSFGILLTEIVTHGRIPYPGMTNPEVIQNLERGYR FT MVRPDNCPEELYHLMMLCWKERPEDRPTFDYLRSVLDDFFTATEGQYQPQPGGGGSGGG FT GSGGGGSGDPPVMVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQ FT TAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFED FT GGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGE FT IKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEG FT RHSTGGMDELYK" XX SQ Sequence 7296 BP; 1758 A; 2003 C; 1874 G; 1661 T; 0 other; aggccctttc gtcttcaaga acagctttgc tcttaggagt ttcctaatac atcccaaact 60 caaatatata aagcatttga cttgttctat gccctagggg gcggggggaa gctaagccag 120 ctttttttaa catttaaaat gttaattcca ttttaaatgc acagatgttt ttatttcata 180 agggtttcaa tgtgcatgaa tgctgcaata ttcctgttac caaagctagt ataaataaaa 240 atagataaac gtggaaatta cttagagttt ctgtcattaa cgtttccttc ctcagttgac 300 aacataaatg cgctgctgag aagccagttt gcatctgtca ggatcaattt cccattatgc 360 cagtcatatt aattactagt caattagttg atttttattt ttgacatata catgtgaaag 420 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa 480 atacataact gagaatagaa aagttcagat caaggtcagg aacagatgga acagctgaat 540 atgggccaaa gcggatatct gtggtaagca gttcctgccc cggctcaggg ccaagaacag 600 atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct 660 cagggccaag aacagatggt ccccagatgc ggtccagccc tcagcagttt ctagagaacc 720 atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa 780 ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct caataaaaga 840 gcccacaacc cctcactcgg ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg 900 tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt tccttgggag 960 ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatttggg ggctcgtccg 1020 ggatcgggag acccctgccc agggaccacc gacccaccac cgggaggtaa gctggccagc 1080 aacttatctg tgtctgtccg attgtctagt gtctatgact gattttatgc gcctgcgtcg 1140 gtactagtta gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttcgg 1200 aacacccggc cgcaaccctg ggagacgtcc cagggacttc gggggccgtt tttgtggccc 1260 gacctgagtc caaaaatccc gatcgttttg gactctttgg tgcacccccc ttagaggagg 1320 gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg tctgaatttt 1380 tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt ctgctgcagc atcgttctgt 1440 gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa tatgggcccg ggccagactg 1500 ttaccactcc cttaagtttg accttaggtc actggaaaga tgtcgagcgg atcgctcaca 1560 accagtcggt agatgtcaag aagagacgtt gggttacctt ctgctctgca gaatggccaa 1620 cctttaacgt cggatggccg cgagacggca cctttaaccg agacctcatc acccaggtta 1680 agatcaaggt cttttcacct ggcccgcatg gacacccaga ccaggtcccc tacatcgtga 1740 cctgggaagc cttggctttt gacccccctc cctgggtcaa gccctttgta caccctaagc 1800 ctccgcctcc tcttcctcca tccgccccgt ctctccccct tgaacctcct cgttcgaccc 1860 cgcctcgatc ctccctttat ccagccctca ctccttctct aggcgccccc atatggccat 1920 atgagatctt atatggggca cccccgcccc ttgtaaactt ccctgaccct gacatgacaa 1980 gagttactaa cagcccctct ctccaagctc acttacaggc tctctactta gtccagcacg 2040 aagtctggag acctctggcg gcagcctacc aagaacaact ggaccgaccg gtggtacctc 2100 acccttaccg agtcggcgac acagtgtggg tccgccgaca ccagactaag aacctagaac 2160 ctcgctggaa aggaccttac acagtcctgc tgaccacccc caccgccctc aaagtagacg 2220 gcatcgcagc ttggatacac gccgcccacg tgaaggctgc cgaccccggg ggtggaccat 2280 cctctagact gccggatccc agtgtggtgg tacgggaatt caagcttgac catgggctgt 2340 gtctgcagct caaaccctga agatgactgg atggagaaca ttgacgtggc tgaaaacgcc 2400 cactatccca tagtcccact ggacagcaag atctcgctgc ccatccggaa tggctctgaa 2460 gtgcgggacc cactggtcac ctatgaggga tctctcccac cagcatcccc gctgcaagac 2520 aacctggtta tcgccctgca cagttatgag ccctcccatg atggagactt gggctttgag 2580 aagggtgaac agctccgaat cctggagcag agcggtgagt ggtggaaggc tcagtccctg 2640 acgactggcc aagaaggctt cattcccttc aacttcgtgg cgaaagcaaa cagcctggag 2700 cctgaacctt ggttcttcaa gaatctgagc cgtaaggacg ccgagcggca gcttttggcg 2760 cccgggaaca cgcatggatc cttcctgatc cgggaaagcg aaagcactgc ggggtccttt 2820 tccctgtcgg tcagagactt cgaccagaac cagggagaag tggtgaaaca ttacaagatc 2880 cgtaacctag acaacggtgg cttctacatc tcccctcgta tcacttttcc cggattgcac 2940 gatctagtcc gccattacac caacgcctct gatgggctgt gcacaaagtt gagccgtcct 3000 tgccagaccc agaagcccca gaaaccatgg tgggaggacg aatgggaagt tcccagggaa 3060 acactgaagt tggtggagcg gctgggagct ggccagttcg gggaagtgtg gatggggtac 3120 tacaacggac acacgaaggt ggcggtgaag agtctgaaac aagggagcat gtcccccgac 3180 gccttcctgg ctgaggctaa cctcatgaag cagctgcagc acccgcggct agtccggctt 3240 tatgcagtgg tcacccagga acccatctac atcatcacgg aatacatgga gaacgggagc 3300 ctagtagatt ttctcaagac tccctcgggc atcaagttga atgtcaacaa acttttggac 3360 atggcagccc agattgcaga gggcatggcg ttcatcgaag aacagaatta catccatcgg 3420 gacctgcgcg ccgccaacat cctggtgtct gacacgctga gctgcaagat tgcagacttt 3480 ggcctggcgc gcctcattga ggacaatgag tacacggccc gggagggggc caaatttccc 3540 attaagtgga cagcaccaga agccattaac tatgggacct tcaccatcaa gtcagacgtg 3600 tggtccttcg ggatcttgct tacagagatc gtcacccacg gtcgaatccc ttacccagga 3660 atgaccaacc ctgaagtcat tcagaacctg gagagaggct accgcatggt gagacctgac 3720 aactgtccgg aagagctgta ccacctcatg atgctgtgct ggaaggagcg cccagaggac 3780 cggcccacgt ttgactacct tcggagtgtt ctggatgact tcttcacagc cacagagggc 3840 cagtaccagc cccagcctgg tggaggcggt agcggtggag gtgggagcgg tggaggtggc 3900 agcggggatc caccggtcat ggtgagcaag ggcgaggagg ataacatggc catcatcaag 3960 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc 4020 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc 4080 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc 4140 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag 4200 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag 4260 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc 4320 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg 4380 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac 4440 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg 4500 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc 4560 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg 4620 tacaagtaaa gcggccgcca gcacagtggt cgacgataaa ataaaagatt ttatttagtc 4680 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa 4740 cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt 4800 caggaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct 4860 gccccggctc agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg 4920 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca 4980 gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat 5040 gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt 5100 ctgctccccg agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga 5160 ttgactgagt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact 5220 tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg 5280 gtctttcatt cacatgcagc atgtatcaaa attaatttgg ttttttttct taagtattta 5340 cattaaatgg ccatagttgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 5400 tattggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg 5460 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac 5520 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg 5580 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca 5640 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc 5700 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc 5760 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag 5820 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc 5880 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca 5940 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg 6000 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg 6060 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct 6120 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa 6180 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa 6240 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa 6300 tgaagtttgc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 6360 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 6420 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 6480 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 6540 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 6600 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 6660 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 6720 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 6780 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 6840 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 6900 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 6960 ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 7020 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 7080 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 7140 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 7200 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 7260 ctcatgacat taacctataa aaataggcgt atcacg 7296 // ID MT628541; SV 1; linear; other DNA; STD; SYN; 1662 BP. XX AC MT628541; XX DT 20-JUL-2020 (Rel. 144, Created) DT 20-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct pectinase gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1662 RA Binay B.; RT ; RL Submitted (16-JUN-2020) to the INSDC. RL Bioengineering, Gebze Technical University, Gebze, Kocaeli, Marmara Bolgesi RL 41400, Turkey XX DR MD5; 593008f84eba9374c3802fd56e9dd8fd. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1662 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Sporothrix schenckii 1099-18 sequence" FT /db_xref="taxon:32630" FT CDS 1..1662 FT /codon_start=1 FT /transl_table=11 FT /product="pectinase" FT /EC_number="3.2.1.67" FT /note="extracellular exo-polygalacturonase; optimized for FT expression in Pichia pastoris" FT /protein_id="QLF97696.1" FT /translation="MRFPSIFTAVLFAASSALAAPVNTTTEDETAQIPAEAVIGYSDLE FT GDFDVAVLPFSNSTNNGLLFINTTIASIAAKEEGVSLEKREAEAYVEFAPSNGNGNGNG FT NDSAPCKPVSTRPTVVAHPKQPRLPIPIPPPRNKVCCVKTHGDGVTDDSTYIMSAFHAC FT NHGGHVVFRADTTYLVGTAMDWTFLKDIDIGGSGRTITVNTVANIPPDIQGTIQFTNDT FT DYWQANSFRFGFQNVTSFFKLGGENVFVYGGGTLHGNGQVWYDLHAQDTDTLRPVLVGI FT DGLQNSVVADLHLRLSPMYYHFVANASNVVFSDIDISISNSNGGASSSAHHAAKNTDGW FT DTYRSSDIAILNSVVVNGDDCVSLKPNSTHILVASLSCTGSHGISIGSLGQYAGAYDIV FT EHVWVGNVSLHAATDGARIKVWPNTPSALSGDLQGGGGGGRVRNITYDGMYIDGVDYAI FT EITQCYGQKNLTVCQQHPSSLTLADVVFRNFYGVTSTKYAPQIAALACSSTATCRAISA FT TNIDVVSPRGGNQAYCLNQNATALDLTCTDVWKGFN" XX SQ Sequence 1662 BP; 387 A; 422 C; 366 G; 487 T; 0 other; atgagatttc cttcaatttt tactgcagtt ttattcgcag catcctccgc attagctgct 60 ccagtcaaca ctacaacaga agatgaaacg gcacaaattc cggctgaagc tgtcatcggt 120 tactcagatt tagaagggga tttcgatgtt gctgttttgc cattttccaa cagcacaaat 180 aacgggttat tgtttataaa tactactatt gccagcattg ctgctaaaga agaaggggta 240 tctctcgaga aaagagaggc tgaagcttac gtagaattcg ctccatctaa cggtaatggt 300 aacggaaacg gtaacgactc cgctccatgt aagccagttt ctactagacc aaccgttgtc 360 gctcatccaa agcaacctag actgccaatt ccaattccac caccaagaaa caaggtctgc 420 tgtgttaaga ctcacggtga cggtgttact gacgactcca cttacattat gtccgctttc 480 cacgcttgta accacggtgg tcacgttgtt tttagagctg acaccaccta cttggtcggt 540 actgctatgg attggacctt cttgaaggac atcgacattg gtggttccgg tagaaccatc 600 accgttaaca ctgttgctaa catcccacca gacatccagg gtactatcca gttcactaac 660 gacactgact actggcaggc taactccttc agattcggtt tccagaacgt cacctcattc 720 ttcaagcttg gaggtgagaa cgtgttcgtt tacggtggtg gtacattgca cggtaacggt 780 caagtttggt acgacttgca cgctcaagac actgacactt tgaggccagt tttggttggt 840 attgacggtc tgcagaactc cgttgttgct gacttgcact tgagactgtc cccaatgtac 900 taccatttcg ttgctaacgc ctccaacgtc gttttctccg acattgacat ttccatctcc 960 aactccaacg gtggtgcttc ttcttctgct catcatgctg ctaagaacac tgacggttgg 1020 gacacttaca gatcctccga tatcgctatc ttgaactccg tcgtcgttaa tggtgacgac 1080 tgtgtttcct tgaagccaaa ctccactcac atcttggttg cttccctgtc ttgtactggt 1140 tcccacggta tttccattgg ttccttgggt caatacgctg gtgcttacga catcgttgaa 1200 cacgtttggg ttggtaacgt ttctctgcac gctgctactg atggtgccag aatcaaggtt 1260 tggcctaaca ctccatctgc tttgtccggt gatcttcaag gtggtggtgg cggtggtaga 1320 gttagaaaca ttacttacga cggtatgtac atcgacggtg tcgactacgc tattgagatc 1380 actcaatgct acggtcagaa gaacttgact gtttgtcagc agcacccatc ttctctgact 1440 ttggccgatg tcgtgttcag aaacttctac ggtgtcacct ccactaagta cgctccacaa 1500 attgctgctt tggcttgttc ctccactgct acttgtagag ctatttccgc taccaacatc 1560 gacgtcgttt cacctagagg tggtaaccag gcctactgtt tgaaccagaa cgctactgcc 1620 ttggacttga cttgtactga cgtttggaag ggtttcaact ga 1662 // ID MT086575; SV 1; circular; other DNA; STD; SYN; 7230 BP. XX AC MT086575; XX DT 30-JUN-2020 (Rel. 144, Created) DT 15-JUL-2020 (Rel. 144, Last updated, Version 2) XX DE Expression vector pMT-5, complete sequence. XX KW . XX OS Expression vector pMT-5 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-7230 RA Taghavi M., Parham A., Dehghani H., Naderi-Meshkin H.; RT ; RL Submitted (17-FEB-2020) to the INSDC. RL Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi RL University of Mashhad, Azadi Square, Mashhad, Razavi Khorasan 91779-48974, RL Iran XX DR MD5; 97837d0445ec4fea51abebccb5266e5a. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..7230 FT /organism="Expression vector pMT-5" FT /mol_type="other DNA" FT /db_xref="taxon:2747878" FT repeat_region 29..172 FT /rpt_family="response_element" FT /rpt_unit_range=29..52 FT /rpt_unit_seq="cacacgtgggttcccgcacgtccg" FT regulatory 29..172 FT /note="sextuplicate hypoxia-response element" FT /regulatory_class="response_element" FT regulatory 188..391 FT /note="derived from human cytomegalovirus immediate early FT promoter" FT /regulatory_class="promoter" FT regulatory 377..385 FT /regulatory_class="TATA_box" FT regulatory 424..466 FT /note="cumate operator sequences (CuO); CymR binding site" FT /regulatory_class="transcriptional_cis_regulatory_region" FT regulatory 487..504 FT /note="Kozak consensus sequence; vertebrate consensus FT sequence for strong initiation of translation" FT /regulatory_class="ribosome_binding_site" FT CDS 505..2556 FT /codon_start=1 FT /transl_table=11 FT /product="cytosine deaminase/uracil FT phosphoribosyltransferase fusion protein" FT /note="product of CodA::upp recombinant fusion gene" FT /protein_id="QKX95816.1" FT /translation="MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARLARQMVSNNA FT LETIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITENSLDAEQGLVIPPFVEPHIH FT LDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQRAWQTLKWQIANGIQHVRTH FT VDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGILSYPNGEALLEEALRLGADVVG FT AIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEIDDEQSRFVETVAALAHHEGMGA FT RVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANPLVNIHLQGRFDTYPKRRGITRVK FT EMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYGQINDGLNLITHHSA FT RTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVRGGKVIASTQPAQTTVYLE FT QPEAIDYKRISGANGVMAKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYE FT ATADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGM FT YRNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV FT AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK" FT sig_peptide 505..609 FT /note="tPA prepro (secretory signal peptide)" FT misc_feature 622..1905 FT /note="cytosine deaminase; CodA" FT misc_feature 1933..2556 FT /note="uracil phosphoribosyltransferase; upp" FT regulatory 2589..3175 FT /note="internal ribosome entry site (IRES); derived from FT encephalomyocarditis virus" FT /regulatory_class="ribosome_binding_site" FT CDS 3176..3895 FT /codon_start=1 FT /transl_table=11 FT /product="enhanced green fluorescent protein" FT /note="mammalian codon-optimized EGFP" FT /protein_id="QKX95817.1" FT /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL FT KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD FT GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK FT VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL FT EFVTAAGITLGMDELYK" FT regulatory 4018..4139 FT /note="SV40 polyadenylation signal" FT /regulatory_class="terminator" FT rep_origin complement(4146..4601) FT /note="derived from f1 bacteriophage" FT regulatory 4628..4732 FT /note="ampicillin resistance promoter" FT /regulatory_class="promoter" FT regulatory 4734..5091 FT /note="SV40 enhancer and early promoter" FT /regulatory_class="promoter" FT CDS 5126..5920 FT /codon_start=1 FT /transl_table=11 FT /product="aminoglycoside phosphotransferase" FT /note="derived from transposon Tn5; confers resistance to FT neomycin, kanamycin, and G418; APH(3')II; kanamycin kinase FT type II; neomycin-kanamycin phosphotransferase type II" FT /protein_id="QKX95818.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT regulatory 6095..6364 FT /note="herpesvirus thymidine kinase polyadenylation signal; FT HSV TK, poly(A) signal" FT /regulatory_class="terminator" FT rep_origin 6512..7131 FT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of FT replication" XX SQ Sequence 7230 BP; 1742 A; 1945 C; 1959 G; 1584 T; 0 other; tagttattaa tagtaaccgc tcgagcggca cacgtgggtt cccgcacgtc cgcacacgtg 60 ggttcccgca cgtccgcaca cgtgggttcc cgcacgtccg cacacgtggg ttcccgcacg 120 tccgcacacg tgggttcccg cacgtccgca cacgtgggtt cccgcacgtc cgccggaatt 180 gatcttcgtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg 240 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca 300 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg 360 tgtacggtgg gaggtctata taagcagagc tacgcgtcga cgtcggccat agcggccgcg 420 gaattgaaaa caaacagaca atctggtctg tttgtattat aagtaaggac tagtccatcg 480 atcgatcccg ccgccaccat ggcgatggat gcaatgaaga gagggctctg ctgtgtgctg 540 ctgctgtgtg gagcagtctt cgtttcgccc agccaggaaa tccatgcccg attcagaaga 600 ggagccagat tggcgcgcca aatggtgtcg aataacgctt tagaaacaat tattaacgcc 660 cggttaccag gcgaagaggg gctgtggcag attcatctgc aggacggaaa aatcagcgcc 720 attgatgcgc aatccggcgt gatgcccata actgaaaaca gcctggatgc cgaacaaggt 780 ttagttatac cgccgtttgt ggagccacat attcacctgg acaccacgca aaccgccgga 840 caaccgaact ggaatcagtc cggcacgctg tttgaaggca ttgaacgctg ggccgagcgc 900 aaagcgttat taacccatga cgatgtgaaa caacgcgcat ggcaaacgct gaaatggcag 960 attgccaacg gcattcagca tgtgcgtacc catgtcgatg tttcggatgc aacgctaact 1020 gcgctgaaag caatgctgga agtgaagcag gaagtcgcgc cgtggattga tctgcaaatc 1080 gtcgccttcc ctcaggaagg gattttgtcg tatcccaacg gtgaagcgtt gctggaagag 1140 gcgttacgct taggggcaga tgtagtgggg gcgattccgc attttgaatt tacccgtgaa 1200 tacggcgtgg agtcgctgca taaaaccttc gccctggcgc aaaaatacga ccgtctcatc 1260 gacgttcact gtgatgagat cgatgacgag cagtcgcgct ttgtcgaaac cgttgctgcc 1320 ctggcgcacc atgaaggcat gggcgcgcga gtcaccgcca gccacaccac ggcaatgcac 1380 tcctataacg gggcgtatac ctcacgcctg ttccgcttgc tgaaaatgtc cggtattaac 1440 tttgtcgcca acccgctggt caatattcat ctgcaaggac gtttcgatac gtatccaaaa 1500 cgtcgcggca tcacgcgcgt taaagagatg ctggagtccg gcattaacgt ctgctttggt 1560 cacgatgatg tcttcgatcc gtggtatccg ctgggaacgg cgaatatgct gcaagtgctg 1620 catatggggc tgcatgtttg ccagttgatg ggctacgggc agattaacga tggcctgaat 1680 ttaatcaccc accacagcgc aaggacgttg aatttgcagg attacggcat tgccgccgga 1740 aacagcgcca acctgattat cctgccggct gaaaatgggt ttgatgcgct gcgccgtcag 1800 gttccggtac gttattcggt acgtggcggc aaggtgattg ccagcacaca accggcacaa 1860 accaccgtat atctggagca gccagaagcc atcgattaca aacgtatcag cggagctaat 1920 ggcgtcatgg ctaagatcgt ggaagtcaaa cacccactcg tcaaacacaa gctgggactg 1980 atgcgtgagc aagatatcag caccaagcgc tttcgcgaac tcgcttccga agtgggtagc 2040 ctgctgactt acgaagcgac cgccgacctc gaaacggaaa aagtaactat cgaaggctgg 2100 aacggcccgg tagaaatcga ccagatcaaa ggtaagaaaa ttaccgttgt gccaattctg 2160 cgtgcgggtc ttggtatgat ggacggtgtg ctggaaaacg ttccgagcgc gcgcatcagc 2220 gttgtcggta tgtaccgtaa tgaagaaacg ctggagccgg taccgtactt ccagaaactg 2280 gtttctaaca tcgatgagcg tatggcgctg atcgttgacc caatgctggc aaccggtggt 2340 tccgttatcg cgaccatcga cctgctgaaa aaagcgggct gcagcagcat caaagttctg 2400 gtgctggtag ctgcgccaga aggtatcgct gcgctggaaa aagcgcaccc ggacgtcgaa 2460 ctgtataccg catcgattga tcagggactg aacgagcacg gatacattat tccgggcctc 2520 ggcgatgccg gtgacaaaat ctttggtacg aaataagctt gtcgaaattc gctagccgcg 2580 cggatccgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa 2640 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg 2700 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc 2760 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct 2820 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac 2880 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc 2940 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta 3000 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg 3060 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg 3120 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatggt 3180 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga 3240 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa 3300 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt 3360 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca 3420 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa 3480 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa 3540 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct 3600 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat 3660 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca 3720 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct 3780 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct 3840 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaaagcgg 3900 ccgcgactct agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 3960 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 4020 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 4080 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 4140 ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 4200 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 4260 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 4320 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 4380 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 4440 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 4500 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 4560 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg 4620 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct 4680 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc 4740 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 4800 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 4860 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 4920 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 4980 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 5040 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca 5100 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc 5160 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc 5220 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga 5280 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac 5340 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct 5400 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa 5460 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc 5520 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct 5580 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc 5640 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg 5700 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct 5760 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct 5820 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca 5880 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa 5940 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc 6000 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc 6060 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag 6120 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac 6180 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat 6240 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac 6300 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc 6360 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 6420 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 6480 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 6540 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 6600 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 6660 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 6720 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 6780 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 6840 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 6900 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 6960 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 7020 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 7080 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 7140 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 7200 tctgtggata accgtattac cgccatgcat 7230 // ID MT086576; SV 1; circular; other DNA; STD; SYN; 7014 BP. XX AC MT086576; XX DT 30-JUN-2020 (Rel. 144, Created) DT 15-JUL-2020 (Rel. 144, Last updated, Version 2) XX DE Expression vector pMT-6, complete sequence. XX KW . XX OS Expression vector pMT-6 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-7014 RA Taghavi M., Parham A., Dehghani H., Naderi-Meshkin H.; RT ; RL Submitted (17-FEB-2020) to the INSDC. RL Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi RL University of Mashhad, Azadi Square, Mashhad, Razavi Khorasan 91779-48974, RL Iran XX DR MD5; 9bfad7f4e3f15955f521cca2217eab36. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..7014 FT /organism="Expression vector pMT-6" FT /mol_type="other DNA" FT /db_xref="taxon:2747879" FT repeat_region 29..172 FT /rpt_family="response_element" FT /rpt_unit_range=29..52 FT /rpt_unit_seq="cacacgtgggttcccgcacgtccg" FT regulatory 29..172 FT /note="sextuplicate hypoxia-response element" FT /regulatory_class="response_element" FT regulatory 208..250 FT /note="cumate operator sequences (CuO); CymR binding site" FT /regulatory_class="transcriptional_cis_regulatory_region" FT regulatory 271..288 FT /note="Kozak consensus sequence; vertebrate consensus FT sequence for strong initiation of translation" FT /regulatory_class="ribosome_binding_site" FT CDS 289..2340 FT /codon_start=1 FT /transl_table=11 FT /product="cytosine deaminase/uracil FT phosphoribosyltransferase fusion protein" FT /note="product of CodA::upp recombinant fusion gene" FT /protein_id="QKX95819.1" FT /translation="MDAMKRGLCCVLLLCGAVFVSPSQEIHARFRRGARLARQMVSNNA FT LETIINARLPGEEGLWQIHLQDGKISAIDAQSGVMPITENSLDAEQGLVIPPFVEPHIH FT LDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVKQRAWQTLKWQIANGIQHVRTH FT VDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGILSYPNGEALLEEALRLGADVVG FT AIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDEIDDEQSRFVETVAALAHHEGMGA FT RVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVANPLVNIHLQGRFDTYPKRRGITRVK FT EMLESGINVCFGHDDVFDPWYPLGTANMLQVLHMGLHVCQLMGYGQINDGLNLITHHSA FT RTLNLQDYGIAAGNSANLIILPAENGFDALRRQVPVRYSVRGGKVIASTQPAQTTVYLE FT QPEAIDYKRISGANGVMAKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYE FT ATADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGM FT YRNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV FT AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK" FT sig_peptide 289..393 FT /note="tPA prepro (secretory signal peptide)" FT misc_feature 406..1689 FT /note="cytosine deaminase; CodA" FT misc_feature 1717..2340 FT /note="uracil phosphoribosyltransferase; upp" FT regulatory 2373..2959 FT /note="internal ribosome entry site (IRES); derived from FT encephalomyocarditis virus" FT /regulatory_class="ribosome_binding_site" FT CDS 2960..3679 FT /codon_start=1 FT /transl_table=11 FT /product="enhanced green fluorescent protein" FT /note="mammalian codon-optimized EGFP" FT /protein_id="QKX95820.1" FT /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL FT KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD FT GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK FT VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL FT EFVTAAGITLGMDELYK" FT regulatory 3802..3923 FT /note="SV40 polyadenylation signal" FT /regulatory_class="terminator" FT rep_origin complement(3930..4385) FT /note="derived from f1 bacteriophage" FT regulatory 4412..4516 FT /note="ampicillin resistance promoter" FT /regulatory_class="promoter" FT regulatory 4518..4875 FT /note="SV40 enhancer and early promoter" FT /regulatory_class="promoter" FT CDS 4910..5704 FT /codon_start=1 FT /transl_table=11 FT /product="aminoglycoside phosphotransferase" FT /note="derived from transposon Tn5; confers resistance to FT neomycin, kanamycin, and G418; APH(3')II; kanamycin kinase FT type II; neomycin-kanamycin phosphotransferase type II" FT /protein_id="QKX95821.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT regulatory 5879..6148 FT /note="herpesvirus thymidine kinase polyadenylation signal, FT HSV TK, poly(A) signal" FT /regulatory_class="terminator" FT rep_origin 6296..6915 FT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of FT replication" XX SQ Sequence 7014 BP; 1691 A; 1896 C; 1897 G; 1530 T; 0 other; tagttattaa tagtaaccgc tcgagcggca cacgtgggtt cccgcacgtc cgcacacgtg 60 ggttcccgca cgtccgcaca cgtgggttcc cgcacgtccg cacacgtggg ttcccgcacg 120 tccgcacacg tgggttcccg cacgtccgca cacgtgggtt cccgcacgtc cgccggaatt 180 tcgacgtcgg ccatagcggc cgcggaattg aaaacaaaca gacaatctgg tctgtttgta 240 ttataagtaa ggactagtcc atcgatcgat cccgccgcca ccatggcgat ggatgcaatg 300 aagagagggc tctgctgtgt gctgctgctg tgtggagcag tcttcgtttc gcccagccag 360 gaaatccatg cccgattcag aagaggagcc agattggcgc gccaaatggt gtcgaataac 420 gctttagaaa caattattaa cgcccggtta ccaggcgaag aggggctgtg gcagattcat 480 ctgcaggacg gaaaaatcag cgccattgat gcgcaatccg gcgtgatgcc cataactgaa 540 aacagcctgg atgccgaaca aggtttagtt ataccgccgt ttgtggagcc acatattcac 600 ctggacacca cgcaaaccgc cggacaaccg aactggaatc agtccggcac gctgtttgaa 660 ggcattgaac gctgggccga gcgcaaagcg ttattaaccc atgacgatgt gaaacaacgc 720 gcatggcaaa cgctgaaatg gcagattgcc aacggcattc agcatgtgcg tacccatgtc 780 gatgtttcgg atgcaacgct aactgcgctg aaagcaatgc tggaagtgaa gcaggaagtc 840 gcgccgtgga ttgatctgca aatcgtcgcc ttccctcagg aagggatttt gtcgtatccc 900 aacggtgaag cgttgctgga agaggcgtta cgcttagggg cagatgtagt gggggcgatt 960 ccgcattttg aatttacccg tgaatacggc gtggagtcgc tgcataaaac cttcgccctg 1020 gcgcaaaaat acgaccgtct catcgacgtt cactgtgatg agatcgatga cgagcagtcg 1080 cgctttgtcg aaaccgttgc tgccctggcg caccatgaag gcatgggcgc gcgagtcacc 1140 gccagccaca ccacggcaat gcactcctat aacggggcgt atacctcacg cctgttccgc 1200 ttgctgaaaa tgtccggtat taactttgtc gccaacccgc tggtcaatat tcatctgcaa 1260 ggacgtttcg atacgtatcc aaaacgtcgc ggcatcacgc gcgttaaaga gatgctggag 1320 tccggcatta acgtctgctt tggtcacgat gatgtcttcg atccgtggta tccgctggga 1380 acggcgaata tgctgcaagt gctgcatatg gggctgcatg tttgccagtt gatgggctac 1440 gggcagatta acgatggcct gaatttaatc acccaccaca gcgcaaggac gttgaatttg 1500 caggattacg gcattgccgc cggaaacagc gccaacctga ttatcctgcc ggctgaaaat 1560 gggtttgatg cgctgcgccg tcaggttccg gtacgttatt cggtacgtgg cggcaaggtg 1620 attgccagca cacaaccggc acaaaccacc gtatatctgg agcagccaga agccatcgat 1680 tacaaacgta tcagcggagc taatggcgtc atggctaaga tcgtggaagt caaacaccca 1740 ctcgtcaaac acaagctggg actgatgcgt gagcaagata tcagcaccaa gcgctttcgc 1800 gaactcgctt ccgaagtggg tagcctgctg acttacgaag cgaccgccga cctcgaaacg 1860 gaaaaagtaa ctatcgaagg ctggaacggc ccggtagaaa tcgaccagat caaaggtaag 1920 aaaattaccg ttgtgccaat tctgcgtgcg ggtcttggta tgatggacgg tgtgctggaa 1980 aacgttccga gcgcgcgcat cagcgttgtc ggtatgtacc gtaatgaaga aacgctggag 2040 ccggtaccgt acttccagaa actggtttct aacatcgatg agcgtatggc gctgatcgtt 2100 gacccaatgc tggcaaccgg tggttccgtt atcgcgacca tcgacctgct gaaaaaagcg 2160 ggctgcagca gcatcaaagt tctggtgctg gtagctgcgc cagaaggtat cgctgcgctg 2220 gaaaaagcgc acccggacgt cgaactgtat accgcatcga ttgatcaggg actgaacgag 2280 cacggataca ttattccggg cctcggcgat gccggtgaca aaatctttgg tacgaaataa 2340 gcttgtcgaa attcgctagc cgcgcggatc cgcccctctc cctccccccc ccctaacgtt 2400 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc 2460 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc 2520 attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag 2580 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg 2640 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat 2700 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga 2760 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc 2820 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg 2880 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga 2940 tgataatatg gccacaacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc 3000 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg 3060 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct 3120 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg 3180 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt 3240 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa 3300 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga 3360 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat 3420 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga 3480 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt 3540 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga 3600 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat 3660 ggacgagctg tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt 3720 gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa 3780 atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc 3840 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg 3900 tccaaactca tcaatgtatc ttaaggcgta aattgtaagc gttaatattt tgttaaaatt 3960 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat 4020 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa 4080 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg 4140 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa 4200 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc 4260 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag 4320 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg 4380 cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 4440 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 4500 ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg 4560 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 4620 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg 4680 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact 4740 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag 4800 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc 4860 ctaggctttt gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca 4920 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg 4980 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg 5040 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc 5100 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt 5160 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc 5220 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca 5280 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc 5340 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg 5400 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct 5460 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc 5520 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc 5580 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta 5640 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt 5700 ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga 5760 gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac 5820 gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg 5880 gggaggctaa ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca 5940 ataaaaagac agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg 6000 tcccagggct ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc 6060 gtttcttcct tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc 6120 aacgtcgggg cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat 6180 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 6240 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 6300 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 6360 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 6420 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 6480 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 6540 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 6600 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 6660 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 6720 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 6780 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 6840 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 6900 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 6960 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat 7014 // ID MT661610; SV 1; linear; other DNA; STD; SYN; 894 BP. XX AC MT661610; XX DT 15-JUL-2020 (Rel. 144, Created) DT 15-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct BKC-b beta-lactamase (blaBKC-b) gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-894 RA Kieffer N., Ebmeyer S., Larsson J.D.G.; RT "The class A carbapanemases BKC-1 and GPC-1 both originate from the RT bacterial genus Shinella"; RL Unpublished. XX RN [2] RP 1-894 RA Kieffer N., Ebmeyer S., Larsson J.D.G.; RT ; RL Submitted (17-JUN-2020) to the INSDC. RL Department of infectious diseases, University of Gothenburg, Guldhedsgatan RL 10, Goteborg, Vastra Gotalands lan [SE-14] 413 46, Suede XX DR MD5; 8fe4698448c6db785169f1e36ba5ac74. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..894 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT gene 1..894 FT /gene="blaBKC-b" FT /note="putative ancestral blaBKC-1" FT CDS 1..894 FT /codon_start=1 FT /transl_table=11 FT /gene="blaBKC-b" FT /product="BKC-b beta-lactamase" FT /note="BKC putative ancestral protein; blaBKC-1 variant; FT variant of the blaBKC-1 gene deleted in vitro of 16 amino FT acid fragment that was actually a duplication; mutant FT BKC/GPC family beta-lactamase; mutant beta-lactamase" FT /protein_id="QLD99528.1" FT /translation="MTITFSRRQAIAGALLAVPAVSTLAASAGAATGGPLEKRLAELEG FT RHKGRIGVAIHNLATGARIGHRADERFLMCSTFKALLAAHILARVDRKEETLDRRIVVG FT KSDLVDWSPVVETRVGGEGISIAELCEAAITLSDNAAANLLLSASGGPKAVTQFLRGFG FT DDVTRLDRTEPTLNYRETPDDERDTTTPAAMAETLRKLIIGDVLARGSKAQLAAWLVMN FT KTGDTRLRAGFPVDWTTGDKTGTNGDRHGNANDVAIAWSPDRGAVVVTAFCEIPGISGD FT ERNAVIAEIGRIAAEA" XX SQ Sequence 894 BP; 150 A; 308 C; 303 G; 133 T; 0 other; atgacgatca cattttcgcg ccggcaggcg attgccggcg ctctccttgc cgttcccgcc 60 gtgtccacgc tggccgccag cgccggggcg gccacgggcg gcccgctgga aaagaggctg 120 gccgagctgg aagggcgtca caagggccgc atcggcgttg ccatacataa tctcgcaacg 180 ggcgcccgca tcggccaccg ggcggacgag cgcttcctga tgtgcagcac cttcaaggcg 240 ttgctggcgg cccatatcct cgcccgcgtc gacaggaagg aagaaacgct cgaccggcgg 300 atcgtcgtcg ggaaatccga tcttgtggac tggtcgccgg tggtggaaac ccgtgtcggc 360 ggcgagggca tctcgattgc cgagctttgc gaggcggcga tcacgctcag cgacaacgcc 420 gcggccaatc tcctgctgtc cgcctcgggc gggccgaagg ccgtcacgca gttcctgcgc 480 ggtttcggcg acgacgtgac gcgcctcgac cgcaccgaac cgacgctcaa ctatcgcgag 540 acgccggacg acgagcgcga cacgaccacg cccgccgcca tggccgagac gctgcgcaag 600 ctgatcatcg gcgatgtcct cgcgcgcggc tcgaaggcgc agcttgccgc ctggctggtg 660 atgaacaaga ccggcgacac gcgattgcgc gccggcttcc cggtcgactg gacgaccggc 720 gacaagaccg gcacgaacgg cgacaggcac ggcaacgcca acgatgtcgc catcgcctgg 780 tcgccggatc gcggcgcggt ggtcgtcacc gctttctgcg agattcccgg catttccggg 840 gatgagcgca acgccgtcat cgccgagatt ggtcgcattg ccgccgaggc ctga 894 // ID AP019390; SV 1; circular; other DNA; STD; SYN; 12317 BP. XX AC AP019390; XX DT 10-JUL-2020 (Rel. 144, Created) DT 10-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Gateway binary vector pB4nVGW3 DNA, complete sequence. XX KW . XX OS Gateway binary vector pB4nVGW3 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-12317 RA Nishimura K., Yoshimura S.; RT ; RL Submitted (15-JAN-2019) to the INSDC. RL Contact:Kohji Nishimura Shimane University, Faculty of Life and RL Environmental Science; 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan XX RN [2] RC Publication Status: Online-Only DOI:10.1093/jxb/erz556 RA Asano T., Nguyen H.-N.T., Michiko Y., Sidiq Y., Nishimura K., Nakashita H., RA Nishiuchi T.; RT "The Arabidopsis MAPKKK ganma-1 is required for full immunity against RT bacterial and fungal infection"; RL J. Exp. Bot. erz556:(2019). XX DR MD5; 22653f7cbf0c362535c415d4db17e030. XX CC constructed using pGWB402 XX FH Key Location/Qualifiers FH FT source 1..12317 FT /organism="Gateway binary vector pB4nVGW3" FT /mol_type="other DNA" FT /db_xref="taxon:2507546" FT misc_feature complement(54..78) FT /note="T-DNA right border" FT regulatory 325..1159 FT /note="35S promoter" FT /regulatory_class="promoter" FT misc_feature 1178..1807 FT /note="n-terminal fragment of yellow fluorescent protein FT (replacement of gcc with aag at 1796-1798)" FT misc_feature complement(1817..1941) FT /note="attR1" FT CDS 2062..2721 FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BBI25408.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS 3063..3368 FT /codon_start=1 FT /transl_table=11 FT /gene="ccdB" FT /protein_id="BBI25409.1" FT /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK FT VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" FT misc_feature 3409..3533 FT /note="attR2" FT regulatory 3543..3952 FT /note="NOS terminator" FT /regulatory_class="terminator" FT regulatory complement(3986..4245) FT /note="NOS terminator" FT /regulatory_class="terminator" FT CDS complement(4635..5429) FT /codon_start=1 FT /transl_table=11 FT /gene="nptII" FT /product="neomycin phosphotransferase II" FT /protein_id="BBI25410.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT regulatory complement(5442..5748) FT /note="NOS promoter" FT /regulatory_class="promoter" FT misc_feature complement(5839..5863) FT /note="T-DNA left border" XX SQ Sequence 12317 BP; 2864 A; 3290 C; 3289 G; 2874 T; 0 other; tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc 60 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga 120 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga 180 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 240 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 300 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc 360 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata 420 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga 480 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag 540 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta 600 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa 660 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca 720 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca 780 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg 840 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa 900 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg 960 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag 1020 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa 1080 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat 1140 ttcatttgga gagaacacgg gggactctag aatgagcatg gtgagcaagg gcgaggagct 1200 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt 1260 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat 1320 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg 1380 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc 1440 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa 1500 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg 1560 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag 1620 ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat 1680 ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc 1740 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct 1800 gagcaaagct gttatcacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat 1860 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac 1920 acaacatatc cagtcactat ggcggcccct cgaggggccg cattaggcac cccaggcttt 1980 acactttatg cttccggctc gtataatgtg tggattttga gttaggatcc gtcgagattt 2040 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata 2100 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat 2160 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac 2220 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc 2280 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc 2340 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc 2400 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat 2460 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc 2520 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg 2580 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat 2640 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat 2700 gagtggcagg gcggggcgta aacgcgtgga tccggcttac taaaagccag ataacagtat 2760 gcgtatttgc gcgctgattt ttgcggtata agaatatata ctgatatgta tacccgaagt 2820 atgtcaaaaa gaggtatgct atgaagcagc gtattacagt gacagttgac agcgacagct 2880 atcagttgct caaggcatat atgatgtcaa tatctccggt ctggtaagca caaccatgca 2940 gaatgaagcc cgtcgtctgc gtgccgaacg ctggaaagcg gaaaatcagg aagggatggc 3000 tgaggtcgcc cggtttattg aaatgaacgg ctcttttgct gacgagaaca ggggctggtg 3060 aaatgcagtt taaggtttac acctataaaa gagagagccg ttatcgtctg tttgtggatg 3120 tacagagtga tattattgac acgcccgggc gacggatggt gatccccctg gccagtgcac 3180 gtctgctgtc agataaagtc tcccgtgaac tttacccggt ggtgcatatc ggggatgaaa 3240 gctggcgcat gatgaccacc gatatggcca gtgtgccggt ctccgttatc ggggaagaag 3300 tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc cattaacctg atgttctggg 3360 gaatataaat gtcaggctcc cttatacaca gccagtctgc aggtcgacca tagtgactgg 3420 atatgttgtg ttttacagta ttatgtagtc tgttttttat gcaaaatcta atttaatata 3480 ttgatattta tatcatttta cgtttctcgt tcagctttct tgtacaaagt ggtgataacc 3540 tagagctcga atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat 3600 cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta 3660 ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg 3720 caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta 3780 tcgcgcgcgg tgtcatctat gttactagat cgggaattgg ttccggaacc aattcgtaat 3840 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac 3900 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattag 3960 gctgaattag gcgcgcctat ttctgaattc ccgatctagt aacatagatg acaccgcgcg 4020 cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa 4080 ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat 4140 tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac 4200 aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga tcatccgggt 4260 ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt 4320 cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgggcccga tcatattgtc 4380 gctcaggatc gtggcgttgt gcttgtcggc cgttgctgtc gtaatgatat cggcaccttc 4440 gaccgcctgt tccgcagaga tcccgtgggc gaagaactcc agcatgagat ccccgcgctg 4500 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc 4560 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa 4620 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa 4680 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct 4740 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg 4800 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca 4860 tcgccatggg tcacgacgag atcatcgccg tcgggcatgc gcgccttgag cctggcgaac 4920 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg 4980 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag 5040 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg 5100 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag 5160 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc 5220 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc 5280 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag 5340 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa 5400 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc cagatccggt gcagattatt 5460 tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag 5520 tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac 5580 gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg 5640 agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt 5700 catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatgaa ttcagaaata 5760 aattcagcct aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag 5820 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct 5880 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga 5940 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt 6000 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca 6060 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt 6120 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact 6180 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt 6240 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt 6300 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc 6360 gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac 6420 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt 6480 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta 6540 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa 6600 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc 6660 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc 6720 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta 6780 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac 6840 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat 6900 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca 6960 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa 7020 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg 7080 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg 7140 tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc 7200 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct 7260 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc 7320 tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc 7380 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 7440 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 7500 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 7560 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 7620 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 7680 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 7740 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 7800 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 7860 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 7920 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 7980 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 8040 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 8100 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 8160 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc 8220 atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg 8280 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg 8340 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta 8400 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc 8460 gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg 8520 cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg 8580 ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg 8640 ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt 8700 taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta 8760 tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg 8820 ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga 8880 gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt 8940 aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc 9000 cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct 9060 tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg 9120 tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa 9180 aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt 9240 tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg 9300 gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata 9360 ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt 9420 ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc 9480 agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc 9540 cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt 9600 aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa 9660 gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca 9720 cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat 9780 ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt 9840 gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt 9900 ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat 9960 cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc 10020 ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc 10080 tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc 10140 ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc 10200 tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt 10260 ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc 10320 gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc 10380 tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac 10440 tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc 10500 ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg 10560 ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc 10620 accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc 10680 atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc 10740 gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc 10800 caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt 10860 cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg 10920 cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc 10980 cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc 11040 tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact 11100 catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac 11160 ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc 11220 ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc 11280 ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac 11340 ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg 11400 cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg 11460 cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca 11520 cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg 11580 cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct 11640 tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa 11700 tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct 11760 ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt 11820 cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta 11880 ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg 11940 aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt 12000 cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt 12060 aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa 12120 aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt 12180 cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg 12240 gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg 12300 gacgaacgga taaacct 12317 // ID AP019391; SV 1; circular; other DNA; STD; SYN; 11774 BP. XX AC AP019391; XX DT 10-JUL-2020 (Rel. 144, Created) DT 10-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Gateway binary vector pB4cVGW DNA, complete sequence. XX KW . XX OS Gateway binary vector pB4cVGW OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-11774 RA Nishimura K., Yoshimura S.; RT ; RL Submitted (15-JAN-2019) to the INSDC. RL Contact:Kohji Nishimura Shimane University, Faculty of Life and RL Environmental Science; 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan XX RN [2] RC Publication Status: Online-Only DOI:10.1093/jxb/erz556 RA Asano T., Nguyen H.-N.T., Michiko Y., Sidiq Y., Nishimura K., Nakashita H., RA Nishiuchi T.; RT "The Arabidopsis MAPKKK ganma-1 is required for full immunity against RT bacterial and fungal infection"; RL J. Exp. Bot. erz556:(2019). XX DR MD5; 67b06e8c38c1c3db46862fdc33988a2d. XX CC constructed using pGWB402 XX FH Key Location/Qualifiers FH FT source 1..11774 FT /organism="Gateway binary vector pB4cVGW" FT /mol_type="other DNA" FT /db_xref="taxon:2507547" FT misc_feature complement(54..78) FT /note="T-DNA right border" FT regulatory 325..1159 FT /note="35S promoter" FT /regulatory_class="promoter" FT misc_feature 1178..1264 FT /note="c-terminal fragment of yellow fluorescent protein" FT misc_feature complement(1274..1398) FT /note="attR1" FT CDS 1519..2178 FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BBI25411.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS 2520..2825 FT /codon_start=1 FT /transl_table=11 FT /gene="ccdB" FT /protein_id="BBI25412.1" FT /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK FT VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" FT misc_feature 2866..2990 FT /note="attR2" FT regulatory 3000..3409 FT /note="NOS terminator" FT /regulatory_class="terminator" FT regulatory complement(3443..3702) FT /note="NOS terminator" FT /regulatory_class="terminator" FT CDS complement(4092..4886) FT /codon_start=1 FT /transl_table=11 FT /gene="nptII" FT /product="neomycin phosphotransferase II" FT /protein_id="BBI25413.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT regulatory complement(4899..5205) FT /note="NOS promoter" FT /regulatory_class="promoter" FT misc_feature complement(5296..5320) FT /note="T-DNA left border" XX SQ Sequence 11774 BP; 2731 A; 3102 C; 3139 G; 2802 T; 0 other; tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc 60 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga 120 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga 180 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 240 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 300 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc 360 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata 420 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga 480 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag 540 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta 600 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa 660 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca 720 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca 780 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg 840 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa 900 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg 960 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag 1020 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa 1080 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat 1140 ttcatttgga gagaacacgg gggactctag aatgagcgac cccaacgaga agcgcgatca 1200 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 1260 caaggctgtt atcacaagtt tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa 1320 tatcaatata ttaaattaga ttttgcataa aaaacagact acataatact gtaaaacaca 1380 acatatccag tcactatggc ggcccctcga ggggccgcat taggcacccc aggctttaca 1440 ctttatgctt ccggctcgta taatgtgtgg attttgagtt aggatccgtc gagattttca 1500 ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt tgatatatcc 1560 caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg tacctataac 1620 cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa taagcacaag 1680 ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc ggaattccgt 1740 atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg ttacaccgtt 1800 ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga cgatttccgg 1860 cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc 1920 cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt gagtttcacc 1980 agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt caccatgggc 2040 aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt tcatcatgcc 2100 gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta ctgcgatgag 2160 tggcagggcg gggcgtaaac gcgtggatcc ggcttactaa aagccagata acagtatgcg 2220 tatttgcgcg ctgatttttg cggtataaga atatatactg atatgtatac ccgaagtatg 2280 tcaaaaagag gtatgctatg aagcagcgta ttacagtgac agttgacagc gacagctatc 2340 agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa ccatgcagaa 2400 tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag ggatggctga 2460 ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg gctggtgaaa 2520 tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt gtggatgtac 2580 agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc agtgcacgtc 2640 tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg gatgaaagct 2700 ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg gaagaagtgg 2760 ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg ttctggggaa 2820 tataaatgtc aggctccctt atacacagcc agtctgcagg tcgaccatag tgactggata 2880 tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt taatatattg 2940 atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt gataacctag 3000 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct 3060 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata 3120 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa 3180 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg 3240 cgcgcggtgt catctatgtt actagatcgg gaattggttc cggaaccaat tcgtaatcat 3300 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 3360 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaggct 3420 gaattaggcg cgcctatttc tgaattcccg atctagtaac atagatgaca ccgcgcgcga 3480 taatttatcc tagtttgcgc gctatatttt gttttctatc gcgtattaaa tgtataattg 3540 cgggactcta atcataaaaa cccatctcat aaataacgtc atgcattaca tgttaattat 3600 tacatgctta acgtaattca acagaaatta tatgataatc atcgcaagac cggcaacagg 3660 attcaatctt aagaaacttt attgccaaat gtttgaacga tcggggatca tccgggtctg 3720 tggcgggaac tccacgaaaa tatccgaacg cagcaagata tcgcggtgca tctcggtctt 3780 gcctgggcag tcgccgccga cgccgttgat gtggacgccg ggcccgatca tattgtcgct 3840 caggatcgtg gcgttgtgct tgtcggccgt tgctgtcgta atgatatcgg caccttcgac 3900 cgcctgttcc gcagagatcc cgtgggcgaa gaactccagc atgagatccc cgcgctggag 3960 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc 4020 ggtggaatcg aaatctcgtg atggcaggtt gggcgtcgct tggtcggtca tttcgaaccc 4080 cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg 4140 ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc aagctcttca 4200 gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc cagccggcca 4260 cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa gcaggcatcg 4320 ccatgggtca cgacgagatc atcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt 4380 tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac aagaccggct 4440 tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta 4500 gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac tttctcggca 4560 ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag cagccagtcc 4620 cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc 4680 cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag gtcggtcttg 4740 acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc agagcagccg 4800 attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc cggagaacct 4860 gcgtgcaatc catcttgttc aatcatgcga aacgatccag atccggtgca gattatttgg 4920 attgagagtg aatatgagac tctaattgga taccgagggg aatttatgga acgtcagtgg 4980 agcatttttg acaagaaata tttgctagct gatagtgacc ttaggcgact tttgaacgcg 5040 caataatggt ttctgacgta tgtgcttagc tcattaaact ccagaaaccc gcggctgagt 5100 ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa aacggcttgt cccgcgtcat 5160 cggcgggggt cataacgtga ctcccttaat tctccgctca tgatgaattc agaaataaat 5220 tcagcctaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt tattaagttg 5280 tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc aacagctccc 5340 cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag tccgggacgg 5400 cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga tgctattcgg 5460 aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag ggtagcatgt 5520 tgattgtaac gatgacagag cgttgctgcc tgtgatcaat tcgggcacga acccagtgga 5580 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt 5640 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt 5700 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg 5760 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc 5820 ctaaaacaaa gttaaacatc atgggggaag cggtgatcgc cgaagtatcg actcaactat 5880 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 5940 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 6000 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 6060 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 6120 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 6180 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 6240 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 6300 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 6360 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 6420 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 6480 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac 6540 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga 6600 aaggcgagat caccaaggta gtcggcaaat aatgtctagc tagaaattcg ttcaagccga 6660 cgccgcttcg cggcgcggct taactcaagc gttagatgca ctaagcacat aattgctcac 6720 agccaaacta tcaggtcaag tctgctttta ttatttttaa gcgtgcataa taagccctac 6780 acaaattggg agatatatca tgcatgacca aaatccctta acgtgagttt tcgttccact 6840 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg 6900 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc 6960 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata 7020 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta 7080 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc 7140 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg 7200 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac 7260 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg 7320 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt 7380 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct 7440 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg 7500 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata 7560 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca 7620 gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc 7680 tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct gatgccgcat 7740 agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca 7800 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 7860 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 7920 acgcgcgagg cagggtgcct tgatgtgggc gccggcggtc gagtggcgac ggcgcggctt 7980 gtccgcgccc tggtagattg cctggccgta ggccagccat ttttgagcgg ccagcggccg 8040 cgataggccg acgcgaagcg gcggggcgta gggagcgcag cgaccgaagg gtaggcgctt 8100 tttgcagctc ttcggctgtg cgctggccag acagttatgc acaggccagg cgggttttaa 8160 gagttttaat aagttttaaa gagttttagg cggaaaaatc gccttttttc tcttttatat 8220 cagtcactta catgtgtgac cggttcccaa tgtacggctt tgggttccca atgtacgggt 8280 tccggttccc aatgtacggc tttgggttcc caatgtacgt gctatccaca ggaaagagac 8340 cttttcgacc tttttcccct gctagggcaa tttgccctag catctgctcc gtacattagg 8400 aaccggcgga tgcttcgccc tcgatcaggt tgcggtagcg catgactagg atcgggccag 8460 cctgccccgc ctcctccttc aaatcgtact ccggcaggtc atttgacccg atcagcttgc 8520 gcacggtgaa acagaacttc ttgaactctc cggcgctgcc actgcgttcg tagatcgtct 8580 tgaacaacca tctggcttct gccttgcctg cggcgcggcg tgccaggcgg tagagaaaac 8640 ggccgatgcc gggatcgatc aaaaagtaat cggggtgaac cgtcagcacg tccgggttct 8700 tgccttctgt gatctcgcgg tacatccaat cagctagctc gatctcgatg tactccggcc 8760 gcccggtttc gctctttacg atcttgtagc ggctaatcaa ggcttcaccc tcggataccg 8820 tcaccaggcg gccgttcttg gccttcttcg tacgctgcat ggcaacgtgc gtggtgttta 8880 accgaatgca ggtttctacc aggtcgtctt tctgctttcc gccatcggct cgccggcaga 8940 acttgagtac gtccgcaacg tgtggacgga acacgcggcc gggcttgtct cccttccctt 9000 cccggtatcg gttcatggat tcggttagat gggaaaccgc catcagtacc aggtcgtaat 9060 cccacacact ggccatgccg gccggccctg cggaaacctc tacgtgcccg tctggaagct 9120 cgtagcggat cacctcgcca gctcgtcggt cacgcttcga cagacggaaa acggccacgt 9180 ccatgatgct gcgactatcg cgggtgccca cgtcatagag catcggaacg aaaaaatctg 9240 gttgctcgtc gcccttgggc ggcttcctaa tcgacggcgc accggctgcc ggcggttgcc 9300 gggattcttt gcggattcga tcagcggccg cttgccacga ttcaccgggg cgtgcttctg 9360 cctcgatgcg ttgccgctgg gcggcctgcg cggccttcaa cttctccacc aggtcatcac 9420 ccagcgccgc gccgatttgt accgggccgg atggtttgcg accgctcacg ccgattcctc 9480 gggcttgggg gttccagtgc cattgcaggg ccggcagaca acccagccgc ttacgcctgg 9540 ccaaccgccc gttcctccac acatggggca ttccacggcg tcggtgcctg gttgttcttg 9600 attttccatg ccgcctcctt tagccgctaa aattcatcta ctcatttatt catttgctca 9660 tttactctgg tagctgcgcg atgtattcag atagcagctc ggtaatggtc ttgccttggc 9720 gtaccgcgta catcttcagc ttggtgtgat cctccgccgg caactgaaag ttgacccgct 9780 tcatggctgg cgtgtctgcc aggctggcca acgttgcagc cttgctgctg cgtgcgctcg 9840 gacggccggc acttagcgtg tttgtgcttt tgctcatttt ctctttacct cattaactca 9900 aatgagtttt gatttaattt cagcggccag cgcctggacc tcgcgggcag cgtcgccctc 9960 gggttctgat tcaagaacgg ttgtgccggc ggcggcagtg cctgggtagc tcacgcgctg 10020 cgtgatacgg gactcaagaa tgggcagctc gtacccggcc agcgcctcgg caacctcacc 10080 gccgatgcgc gtgcctttga tcgcccgcga cacgacaaag gccgcttgta gccttccatc 10140 cgtgacctca atgcgctgct taaccagctc caccaggtcg gcggtggccc atatgtcgta 10200 agggcttggc tgcaccggaa tcagcacgaa gtcggctgcc ttgatcgcgg acacagccaa 10260 gtccgccgcc tggggcgctc cgtcgatcac tacgaagtcg cgccggccga tggccttcac 10320 gtcgcggtca atcgtcgggc ggtcgatgcc gacaacggtt agcggttgat cttcccgcac 10380 ggccgcccaa tcgcgggcac tgccctgggg atcggaatcg actaacagaa catcggcccc 10440 ggcgagttgc agggcgcggg ctagatgggt tgcgatggtc gtcttgcctg acccgccttt 10500 ctggttaagt acagcgataa ccttcatgcg ttccccttgc gtatttgttt atttactcat 10560 cgcatcatat acgcagcgac cgcatgacgc aagctgtttt actcaaatac acatcacctt 10620 tttagacggc ggcgctcggt ttcttcagcg gccaagctgg ccggccaggc cgccagcttg 10680 gcatcagaca aaccggccag gatttcatgc agccgcacgg ttgagacgtg cgcgggcggc 10740 tcgaacacgt acccggccgc gatcatctcc gcctcgatct cttcggtaat gaaaaacggt 10800 tcgtcctggc cgtcctggtg cggtttcatg cttgttcctc ttggcgttca ttctcggcgg 10860 ccgccagggc gtcggcctcg gtcaatgcgt cctcacggaa ggcaccgcgc cgcctggcct 10920 cggtgggcgt cacttcctcg ctgcgctcaa gtgcgcggta cagggtcgag cgatgcacgc 10980 caagcagtgc agccgcctct ttcacggtgc ggccttcctg gtcgatcagc tcgcgggcgt 11040 gcgcgatctg tgccggggtg agggtagggc gggggccaaa cttcacgcct cgggccttgg 11100 cggcctcgcg cccgctccgg gtgcggtcga tgattaggga acgctcgaac tcggcaatgc 11160 cggcgaacac ggtcaacacc atgcggccgg ccggcgtggt ggtgtcggcc cacggctctg 11220 ccaggctacg caggcccgcg ccggcctcct ggatgcgctc ggcaatgtcc agtaggtcgc 11280 gggtgctgcg ggccaggcgg tctagcctgg tcactgtcac aacgtcgcca gggcgtaggt 11340 ggtcaagcat cctggccagc tccgggcggt cgcgcctggt gccggtgatc ttctcggaaa 11400 acagcttggt gcagccggcc gcgtgcagtt cggcccgttg gttggtcaag tcctggtcgt 11460 cggtgctgac gcgggcatag cccagcaggc cagcggcggc gctcttgttc atggcgtaat 11520 gtctccggtt ctagtcgcaa gtattctact ttatgcgact aaaacacgcg acaagaaaac 11580 gccaggaaaa gggcagggcg gcagcctgtc gcgtaactta ggacttgtgc gacatgtcgt 11640 tttcagaaga cggctgcact gaacgtcaga agccgactgc actatagcag cggaggggtt 11700 ggatcaaagt actttgatcc cgaggggaac cctgtggttg gcatgcacat acaaatggac 11760 gaacggataa acct 11774 // ID AP019392; SV 1; circular; other DNA; STD; SYN; 12315 BP. XX AC AP019392; XX DT 10-JUL-2020 (Rel. 144, Created) DT 10-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Gateway binary vector pB4GWnV3 DNA, complete sequence. XX KW . XX OS Gateway binary vector pB4GWnV3 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-12315 RA Nishimura K., Yoshimura S.; RT ; RL Submitted (15-JAN-2019) to the INSDC. RL Contact:Kohji Nishimura Shimane University, Faculty of Life and RL Environmental Science; 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan XX RN [2] RC Publication Status: Online-Only DOI:10.1093/jxb/erz556 RA Asano T., Nguyen H.-N.T., Michiko Y., Sidiq Y., Nishimura K., Nakashita H., RA Nishiuchi T.; RT "The Arabidopsis MAPKKK ganma-1 is required for full immunity against RT bacterial and fungal infection"; RL J. Exp. Bot. erz556:(2019). XX DR MD5; 8c5e5f5e605794fc9ead1cf08020cb67. XX CC constructed using pGWB402 XX FH Key Location/Qualifiers FH FT source 1..12315 FT /organism="Gateway binary vector pB4GWnV3" FT /mol_type="other DNA" FT /db_xref="taxon:2507548" FT misc_feature complement(54..78) FT /note="T-DNA right border" FT regulatory 325..1159 FT /note="35S promoter" FT /regulatory_class="promoter" FT misc_feature complement(1179..1303) FT /note="attR1" FT CDS 1424..2083 FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BBI25414.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS 2425..2730 FT /codon_start=1 FT /transl_table=11 FT /gene="ccdB" FT /protein_id="BBI25415.1" FT /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK FT VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" FT misc_feature 2771..2895 FT /note="attR2" FT misc_feature 2906..3535 FT /note="n-terminal fragment of yellow fluorescent protein FT (replacement of gcc with aag at 3524-3426)" FT regulatory 3541..3950 FT /note="NOS terminator" FT /regulatory_class="terminator" FT regulatory complement(3984..4243) FT /note="NOS terminator" FT /regulatory_class="terminator" FT CDS complement(4633..5427) FT /codon_start=1 FT /transl_table=11 FT /gene="nptII" FT /product="neomycin phosphotransferase II" FT /protein_id="BBI25416.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT regulatory complement(5440..5746) FT /note="NOS promoter" FT /regulatory_class="promoter" FT misc_feature complement(5837..5861) FT /note="T-DNA left border" XX SQ Sequence 12315 BP; 2865 A; 3288 C; 3287 G; 2875 T; 0 other; tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc 60 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga 120 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga 180 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 240 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 300 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc 360 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata 420 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga 480 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag 540 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta 600 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa 660 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca 720 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca 780 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg 840 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa 900 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg 960 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag 1020 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa 1080 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat 1140 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg 1200 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac 1260 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc 1320 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt 1380 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac 1440 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca 1500 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa 1560 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct 1620 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga 1680 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg 1740 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg 1800 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc 1860 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt 1920 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc 1980 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa 2040 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt 2100 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata 2160 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca 2220 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg 2280 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag 2340 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg 2400 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc 2460 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg 2520 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg 2580 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg 2640 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac 2700 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct 2760 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt 2820 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt 2880 cttgtacaaa gtggttgata acagcatggt gagcaagggc gaggagctgt tcaccggggt 2940 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 3000 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg 3060 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcc tgcagtgctt 3120 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 3180 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 3240 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 3300 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 3360 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat 3420 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 3480 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagctta 3540 gagctcgaat ttccccgatc gttcaaacat ttggcaataa agtttcttaa gattgaatcc 3600 tgttgccggt cttgcgatga ttatcatata atttctgttg aattacgtta agcatgtaat 3660 aattaacatg taatgcatga cgttatttat gagatgggtt tttatgatta gagtcccgca 3720 attatacatt taatacgcga tagaaaacaa aatatagcgc gcaaactagg ataaattatc 3780 gcgcgcggtg tcatctatgt tactagatcg ggaattggtt ccggaaccaa ttcgtaatca 3840 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 3900 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaggc 3960 tgaattaggc gcgcctattt ctgaattccc gatctagtaa catagatgac accgcgcgcg 4020 ataatttatc ctagtttgcg cgctatattt tgttttctat cgcgtattaa atgtataatt 4080 gcgggactct aatcataaaa acccatctca taaataacgt catgcattac atgttaatta 4140 ttacatgctt aacgtaattc aacagaaatt atatgataat catcgcaaga ccggcaacag 4200 gattcaatct taagaaactt tattgccaaa tgtttgaacg atcggggatc atccgggtct 4260 gtggcgggaa ctccacgaaa atatccgaac gcagcaagat atcgcggtgc atctcggtct 4320 tgcctgggca gtcgccgccg acgccgttga tgtggacgcc gggcccgatc atattgtcgc 4380 tcaggatcgt ggcgttgtgc ttgtcggccg ttgctgtcgt aatgatatcg gcaccttcga 4440 ccgcctgttc cgcagagatc ccgtgggcga agaactccag catgagatcc ccgcgctgga 4500 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg 4560 cggtggaatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc 4620 ccagagtccc gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc 4680 gggagcggcg ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc 4740 agcaatatca cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc 4800 acagtcgatg aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc 4860 gccatgggtc acgacgagat catcgccgtc gggcatgcgc gccttgagcc tggcgaacag 4920 ttcggctggc gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc 4980 ttccatccga gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt 5040 agccggatca agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc 5100 aggagcaagg tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc 5160 ccttcccgct tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag 5220 ccacgatagc cgcgctgcct cgtcctgcag ttcattcagg gcaccggaca ggtcggtctt 5280 gacaaaaaga accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc 5340 gattgtctgt tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc 5400 tgcgtgcaat ccatcttgtt caatcatgcg aaacgatcca gatccggtgc agattatttg 5460 gattgagagt gaatatgaga ctctaattgg ataccgaggg gaatttatgg aacgtcagtg 5520 gagcattttt gacaagaaat atttgctagc tgatagtgac cttaggcgac ttttgaacgc 5580 gcaataatgg tttctgacgt atgtgcttag ctcattaaac tccagaaacc cgcggctgag 5640 tggctccttc aacgttgcgg ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca 5700 tcggcggggg tcataacgtg actcccttaa ttctccgctc atgatgaatt cagaaataaa 5760 ttcagcctaa ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt 5820 gtctaagcgt caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc 5880 ccgaccggca gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg 5940 gcgtcagcgg gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg 6000 gaagaacggc aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg 6060 ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa ttcgggcacg aacccagtgg 6120 acataagcct gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg 6180 tccagaacct tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt 6240 tatgactgtt tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac 6300 gccgtgggtc gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc 6360 cctaaaacaa agttaaacat catgggggaa gcggtgatcg ccgaagtatc gactcaacta 6420 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg 6480 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg 6540 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact 6600 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac 6660 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag 6720 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc 6780 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc 6840 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg 6900 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt 6960 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg 7020 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga 7080 caagaagaag atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg 7140 aaaggcgaga tcaccaaggt agtcggcaaa taatgtctag ctagaaattc gttcaagccg 7200 acgccgcttc gcggcgcggc ttaactcaag cgttagatgc actaagcaca taattgctca 7260 cagccaaact atcaggtcaa gtctgctttt attattttta agcgtgcata ataagcccta 7320 cacaaattgg gagatatatc atgcatgacc aaaatccctt aacgtgagtt ttcgttccac 7380 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc 7440 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat 7500 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat 7560 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct 7620 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt 7680 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg 7740 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta 7800 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg 7860 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg 7920 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 7980 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 8040 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 8100 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 8160 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat 8220 ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca 8280 tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac 8340 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca 8400 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga 8460 aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct 8520 tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc 8580 gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct 8640 ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta 8700 agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata 8760 tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg 8820 ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaagaga 8880 ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag 8940 gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca 9000 gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg 9060 cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc 9120 ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa 9180 cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc 9240 ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc 9300 cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc 9360 gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt 9420 aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag 9480 aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct 9540 tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa 9600 tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc 9660 tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg 9720 tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct 9780 ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc 9840 cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct 9900 gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca 9960 cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct 10020 cgggcttggg ggttccagtg ccattgcagg gccggcagac aacccagccg cttacgcctg 10080 gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt 10140 gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc 10200 atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg 10260 cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc 10320 ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc 10380 ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc 10440 aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct 10500 cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct 10560 gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac 10620 cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat 10680 ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt 10740 aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca 10800 agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca 10860 cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca 10920 cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc 10980 cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt 11040 tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca 11100 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct 11160 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt 11220 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg 11280 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg 11340 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg 11400 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc 11460 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg 11520 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg 11580 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg 11640 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg 11700 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct 11760 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg 11820 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg 11880 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa 11940 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg 12000 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa 12060 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa 12120 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg 12180 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt 12240 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga 12300 cgaacggata aacct 12315 // ID AP019393; SV 1; circular; other DNA; STD; SYN; 11772 BP. XX AC AP019393; XX DT 10-JUL-2020 (Rel. 144, Created) DT 10-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Gateway binary vector pB4GWcV DNA, complete sequence. XX KW . XX OS Gateway binary vector pB4GWcV OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-11772 RA Nishimura K., Yoshimura S.; RT ; RL Submitted (15-JAN-2019) to the INSDC. RL Contact:Kohji Nishimura Shimane University, Faculty of Life and RL Environmental Science; 1060 Nishikawatsu, Matsue, Shimane 690-8504, Japan XX RN [2] RC Publication Status: Online-Only DOI:10.1093/jxb/erz556 RA Asano T., Nguyen H.-N.T., Michiko Y., Sidiq Y., Nishimura K., Nakashita H., RA Nishiuchi T.; RT "The Arabidopsis MAPKKK ganma-1 is required for full immunity against RT bacterial and fungal infection"; RL J. Exp. Bot. erz556:(2019). XX DR MD5; 6c79ac2545455304b6bea038f10cfd69. XX CC constructed using pGWB402 XX FH Key Location/Qualifiers FH FT source 1..11772 FT /organism="Gateway binary vector pB4GWcV" FT /mol_type="other DNA" FT /db_xref="taxon:2507549" FT misc_feature complement(54..78) FT /note="T-DNA right border" FT regulatory 325..1159 FT /note="35S promoter" FT /regulatory_class="promoter" FT misc_feature complement(1179..1303) FT /note="attR1" FT CDS 1424..2083 FT /codon_start=1 FT /transl_table=11 FT /gene="cat" FT /product="chloramphenicol acetyltransferase" FT /protein_id="BBI25417.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" FT CDS 2425..2730 FT /codon_start=1 FT /transl_table=11 FT /gene="ccdB" FT /protein_id="BBI25418.1" FT /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK FT VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" FT misc_feature 2771..2895 FT /note="attR2" FT misc_feature 2906..2992 FT /gene="c-terminal fragment of yellow fluorescent protein" FT regulatory 2998..3407 FT /note="NOS terminator" FT /regulatory_class="terminator" FT regulatory complement(3441..3700) FT /note="NOS terminator" FT /regulatory_class="terminator" FT CDS complement(4090..4884) FT /codon_start=1 FT /transl_table=11 FT /gene="nptII" FT /product="neomycin phosphotransferase II" FT /protein_id="BBI25419.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT regulatory complement(4897..5203) FT /note="NOS promoter" FT /regulatory_class="promoter" FT misc_feature complement(5294..5318) FT /note="T-DNA left border" XX SQ Sequence 11772 BP; 2732 A; 3100 C; 3137 G; 2803 T; 0 other; tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc 60 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga 120 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga 180 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 240 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc 300 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc 360 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata 420 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga 480 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag 540 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta 600 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa 660 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca 720 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca 780 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg 840 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa 900 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg 960 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag 1020 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa 1080 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat 1140 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg 1200 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac 1260 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc 1320 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt 1380 gagttaggat ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac 1440 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca 1500 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa 1560 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct 1620 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga 1680 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg 1740 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg 1800 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc 1860 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt 1920 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc 1980 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa 2040 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt 2100 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata 2160 tactgatatg tatacccgaa gtatgtcaaa aagaggtatg ctatgaagca gcgtattaca 2220 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg 2280 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag 2340 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg 2400 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc 2460 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg 2520 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg 2580 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg 2640 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac 2700 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct 2760 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt 2820 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt 2880 cttgtacaaa gtggttgata acagcgaccc caacgagaag cgcgatcaca tggtcctgct 2940 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca aggcttagag 3000 ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt 3060 tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat 3120 taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt 3180 atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg 3240 cgcggtgtca tctatgttac tagatcggga attggttccg gaaccaattc gtaatcatgg 3300 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 3360 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaggctga 3420 attaggcgcg cctatttctg aattcccgat ctagtaacat agatgacacc gcgcgcgata 3480 atttatccta gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg 3540 ggactctaat cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta 3600 catgcttaac gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat 3660 tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc ggggatcatc cgggtctgtg 3720 gcgggaactc cacgaaaata tccgaacgca gcaagatatc gcggtgcatc tcggtcttgc 3780 ctgggcagtc gccgccgacg ccgttgatgt ggacgccggg cccgatcata ttgtcgctca 3840 ggatcgtggc gttgtgcttg tcggccgttg ctgtcgtaat gatatcggca ccttcgaccg 3900 cctgttccgc agagatcccg tgggcgaaga actccagcat gagatccccg cgctggagga 3960 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg 4020 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca 4080 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg 4140 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc 4200 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca 4260 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc 4320 atgggtcacg acgagatcat cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc 4380 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc 4440 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc 4500 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg 4560 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct 4620 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca 4680 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac 4740 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat 4800 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc 4860 gtgcaatcca tcttgttcaa tcatgcgaaa cgatccagat ccggtgcaga ttatttggat 4920 tgagagtgaa tatgagactc taattggata ccgaggggaa tttatggaac gtcagtggag 4980 catttttgac aagaaatatt tgctagctga tagtgacctt aggcgacttt tgaacgcgca 5040 ataatggttt ctgacgtatg tgcttagctc attaaactcc agaaacccgc ggctgagtgg 5100 ctccttcaac gttgcggttc tgtcagttcc aaacgtaaaa cggcttgtcc cgcgtcatcg 5160 gcgggggtca taacgtgact cccttaattc tccgctcatg atgaattcag aaataaattc 5220 agcctaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta ttaagttgtc 5280 taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa cagctccccg 5340 accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc cgggacggcg 5400 tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg ctattcggaa 5460 gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg tagcatgttg 5520 attgtaacga tgacagagcg ttgctgcctg tgatcaattc gggcacgaac ccagtggaca 5580 taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc 5640 agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat 5700 gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc 5760 gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct 5820 aaaacaaagt taaacatcat gggggaagcg gtgatcgccg aagtatcgac tcaactatca 5880 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac 5940 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg 6000 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg 6060 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac 6120 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc 6180 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg 6240 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt 6300 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac 6360 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg 6420 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag 6480 cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa 6540 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa 6600 ggcgagatca ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt caagccgacg 6660 ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa ttgctcacag 6720 ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata agccctacac 6780 aaattgggag atatatcatg catgaccaaa atcccttaac gtgagttttc gttccactga 6840 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta 6900 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa 6960 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact 7020 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca 7080 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt 7140 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg 7200 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag 7260 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta 7320 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat 7380 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg 7440 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc 7500 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac 7560 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc 7620 gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg 7680 tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag 7740 ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc 7800 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac 7860 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac 7920 gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg cgcggcttgt 7980 ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc agcggccgcg 8040 ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt aggcgctttt 8100 tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg ggttttaaga 8160 gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc ttttatatca 8220 gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat gtacgggttc 8280 cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg aaagagacct 8340 tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt acattaggaa 8400 ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat cgggccagcc 8460 tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat cagcttgcgc 8520 acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta gatcgtcttg 8580 aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta gagaaaacgg 8640 ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc cgggttcttg 8700 ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta ctccggccgc 8760 ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc ggataccgtc 8820 accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt ggtgtttaac 8880 cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg ccggcagaac 8940 ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc cttcccttcc 9000 cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag gtcgtaatcc 9060 cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc tggaagctcg 9120 tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac ggccacgtcc 9180 atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa aaaatctggt 9240 tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg cggttgccgg 9300 gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg tgcttctgcc 9360 tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag gtcatcaccc 9420 agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc gattcctcgg 9480 gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt acgcctggcc 9540 aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt tgttcttgat 9600 tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca tttgctcatt 9660 tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt gccttggcgt 9720 accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt gacccgcttc 9780 atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg tgcgctcgga 9840 cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca ttaactcaaa 9900 tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg tcgccctcgg 9960 gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc acgcgctgcg 10020 tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca acctcaccgc 10080 cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc cttccatccg 10140 tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat atgtcgtaag 10200 ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac acagccaagt 10260 ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg gccttcacgt 10320 cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct tcccgcacgg 10380 ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca tcggccccgg 10440 cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac ccgcctttct 10500 ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat ttactcatcg 10560 catcatatac gcagcgaccg catgacgcaa gctgttttac tcaaatacac atcacctttt 10620 tagacggcgg cgctcggttt cttcagcggc caagctggcc ggccaggccg ccagcttggc 10680 atcagacaaa ccggccagga tttcatgcag ccgcacggtt gagacgtgcg cgggcggctc 10740 gaacacgtac ccggccgcga tcatctccgc ctcgatctct tcggtaatga aaaacggttc 10800 gtcctggccg tcctggtgcg gtttcatgct tgttcctctt ggcgttcatt ctcggcggcc 10860 gccagggcgt cggcctcggt caatgcgtcc tcacggaagg caccgcgccg cctggcctcg 10920 gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca gggtcgagcg atgcacgcca 10980 agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt cgatcagctc gcgggcgtgc 11040 gcgatctgtg ccggggtgag ggtagggcgg gggccaaact tcacgcctcg ggccttggcg 11100 gcctcgcgcc cgctccgggt gcggtcgatg attagggaac gctcgaactc ggcaatgccg 11160 gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg tgtcggccca cggctctgcc 11220 aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg caatgtccag taggtcgcgg 11280 gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa cgtcgccagg gcgtaggtgg 11340 tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc cggtgatctt ctcggaaaac 11400 agcttggtgc agccggccgc gtgcagttcg gcccgttggt tggtcaagtc ctggtcgtcg 11460 gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc tcttgttcat ggcgtaatgt 11520 ctccggttct agtcgcaagt attctacttt atgcgactaa aacacgcgac aagaaaacgc 11580 caggaaaagg gcagggcggc agcctgtcgc gtaacttagg acttgtgcga catgtcgttt 11640 tcagaagacg gctgcactga acgtcagaag ccgactgcac tatagcagcg gaggggttgg 11700 atcaaagtac tttgatcccg aggggaaccc tgtggttggc atgcacatac aaatggacga 11760 acggataaac ct 11772 // ID MK310264; SV 1; linear; other RNA; STD; SYN; 1209 BP. XX AC MK310264; XX DT 10-JUL-2020 (Rel. 144, Created) DT 10-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct red fluorescent protein based redox biosensor GrxRFP1 DE gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1209 RA Fan Y., Ai H.-W.; RT ; RL Submitted (17-DEC-2018) to the INSDC. RL Molecular Physiology and Biological Physics, University of Virginia, 1340 RL Jefferson Park Ave, Charlottesville, VA 22908, USA XX DR MD5; f494e3456c3fe674f4b292b9a251fd7f. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1209 FT /organism="synthetic construct" FT /mol_type="other RNA" FT /db_xref="taxon:32630" FT CDS 1..1209 FT /codon_start=1 FT /transl_table=11 FT /product="red fluorescent protein based redox biosensor FT GrxRFP1" FT /protein_id="QDH45908.1" FT /translation="MAQEFVNCKIQPGKVVVFIKPTCPYCRRAQEILSQLPIKQGLLEF FT VDITATNHTNEIQDYLQQLTGARTVPQVFISKDCIGGCSDLVSLQQSGELLTRLKQIGA FT LQTGGSGGGSSGGGGSGGGGSGGGGSGRGGSGGELSCAISRLSSPVVSERMYPEDGALK FT SEIKKGLRLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKLDIVSHNEDYTIVEQYERA FT EGRHSTGGMDELYKGGTGGSLVSKGEEDNMAIIKEFMRFKVHMEGSVNGYEFEIEGEGE FT GRPYEAFQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGFRW FT ERVMNFEDGGIIHVNQDSSLQDGVFIYKVKLRGTNFPPDGPVMQKKTMGWEATRDQRCS FT SQA" XX SQ Sequence 1209 BP; 285 A; 324 C; 387 G; 213 T; 0 other; atggctcaag agtttgtgaa ctgcaaaatc cagcctggga aggtggttgt gttcatcaag 60 cccacctgcc cgtactgcag gagggcccaa gagatcctca gtcaattgcc catcaaacaa 120 gggcttctgg aatttgtcga tatcacagcc accaaccaca ctaacgagat tcaagattat 180 ttgcaacagc tcacgggagc aagaacggtg cctcaagtct ttattagtaa agattgtata 240 ggcggatgca gtgatctagt ctctttgcaa cagagtgggg aactgctgac gcggctaaag 300 cagattggag ctctgcagac tggtggcagt ggtggcggca gtagtggcgg tggagggagc 360 ggaggggggg gaagcggggg aggtggaagt ggcaggggag gtagtggggg cgagcttagt 420 tgcgctataa gtcggctgag ctcacccgtg gtctccgagc ggatgtaccc cgaggacggc 480 gccctgaaga gcgagatcaa gaaggggctg aggctgaagg acggcggcca ctacgccgcc 540 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc 600 gacatcaagt tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa 660 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg aggtacaggc 720 gggagtctgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 780 ttcaaggtgc acatggaggg ctccgtgaac ggctacgagt tcgagatcga gggcgagggc 840 gagggccgcc cctacgaggc ctttcagacc gctaagctga aggtgaccaa gggtggcccc 900 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacatt 960 aagcacccag ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaggtgg 1020 gagcgcgtga tgaacttcga ggacggcggc attattcacg ttaaccagga ctcctccctg 1080 caggacggcg tattcatcta caaggtgaag ctgcgcggca ccaacttccc ccccgacggc 1140 cccgtaatgc agaagaagac catgggctgg gaggctacgc gtgaccaaag gtgctccagt 1200 caggcttaa 1209 // ID MK310265; SV 1; linear; other RNA; STD; SYN; 1569 BP. XX AC MK310265; XX DT 10-JUL-2020 (Rel. 144, Created) DT 10-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct red fluorescent protein based redox biosensor PerxRFP1 DE gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1569 RA Fan Y., Ai H.-W.; RT ; RL Submitted (17-DEC-2018) to the INSDC. RL Molecular Physiology and Biological Physics, University of Virginia, 1340 RL Jefferson Park Ave, Charlottesville, VA 22908, USA XX DR MD5; 4713d5219e155ce510e223e7d51c7ed8. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1569 FT /organism="synthetic construct" FT /mol_type="other RNA" FT /db_xref="taxon:32630" FT CDS 1..1569 FT /codon_start=1 FT /transl_table=11 FT /product="red fluorescent protein based redox biosensor FT PerxRFP1" FT /protein_id="QDH45909.1" FT /translation="MASQQGETMSGPLHIGLIPTVGPYLLPHIIPMLHQTFPKLEMYLH FT EAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPLFDEPMLLAIYEDHPWANRECVPMA FT DLAGEKLLMLEDGHCLRDQAMGFSFEAGADEDTHFRATSLETLRNMVAAGSGITLLPAL FT AVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGSPLRSRYEQLAEAICARMDGHFDKVLK FT QAVEFGGSGGGGSGGGGSGGGGSGGGGSGGGGSGGELSCAISRLSSPVVSERMYPEDGA FT LKSEIKKGLRLKDGGHYAAEVKTTYKAKKPVQLPGAYIVDIKMDIVSHNEDYTIVEQYE FT RAEGRHSTGGMDELYKGGTGGSLVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGE FT GEGRPYEAFQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYIKHPADIPDYFKLSFPEGF FT RWERVMNFEEGGIIHVNQDSSLQDGVFIYKVKLRGTNFPPDGPVMQKKTMGWEYTRDQR FT CSSQA" XX SQ Sequence 1569 BP; 351 A; 423 C; 508 G; 287 T; 0 other; atggcaagcc agcagggcga gacgatgtcc ggaccgctgc acattggttt gattcccaca 60 gttggaccgt acctgctacc gcatattatc cctatgctgc accagacctt tccaaagctg 120 gaaatgtatc tgcatgaagc acagacccac cagttactgg cgcaactgga cagcggcaaa 180 ctcgattgcg tgatcctcgc gctggtgaaa gagagcgaag cattcattga agtgccgttg 240 tttgatgagc caatgttgct ggctatctat gaagatcacc cgtgggcgaa ccgcgaatgc 300 gtaccgatgg ccgatctggc aggggaaaaa ctgctgatgc tggaagatgg tcactgtttg 360 cgcgatcagg caatgggttt cagttttgaa gccggggcgg atgaagatac acacttccgc 420 gcgaccagcc tggaaactct gcgcaacatg gtggcggcag gtagcgggat cactttactg 480 ccagcgctgg ctgtgccgcc ggagcgcaaa cgcgatgggg ttgtttatct gccgtgcatt 540 aagccggaac cacgccgcac tattggcctg gtttatcgtc ctggctcacc gctgcgcagc 600 cgctatgagc agctggcaga ggccatctgc gcaagaatgg atggccattt cgataaagtt 660 ttaaaacagg cggttgaatt cggtggcagt ggtggcggcg gtagtggggg cggagggagc 720 ggaggggggg gaagcggggg aggtggaagt ggcgggggag gtagtggggg cgagcttagt 780 tgcgctataa gtcggctgag ctcacccgtg gtttccgagc ggatgtaccc cgaggacggc 840 gccctgaaga gcgagatcaa gaaggggctg aggctgaagg acggcggcca ctacgccgcc 900 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc 960 gacatcaaga tggacatcgt gtcccacaac gaggactaca ccatcgtgga acagtacgaa 1020 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaaggg aggtacaggc 1080 gggagtctgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc 1140 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc 1200 gagggccgcc cctacgaggc ctttcagacc gctaagctga aggtgaccaa gggtggcccc 1260 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacatt 1320 aagcacccag ccgacatccc cgactacttc aagctgtcct tccccgaggg cttcaggtgg 1380 gagcgcgtga tgaacttcga ggagggcggc attattcacg ttaaccagga ctcctccctg 1440 caggacggcg tattcatcta caaggtgaag ctgcgcggca ccaacttccc ccccgacggc 1500 cccgtaatgc agaagaagac catgggctgg gagtatacgc gtgaccaaag gtgctccagt 1560 caggcttaa 1569 // ID MN561360; SV 1; linear; other RNA; STD; SYN; 2451 BP. XX AC MN561360; XX DT 10-JUL-2020 (Rel. 144, Created) DT 10-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct SUS1 gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-2451 RA Xue F., Jiang M.; RT "Salidroside production by in-situ UDP-glucose regeneration"; RL Unpublished. XX RN [2] RP 1-2451 RA Xue F., Jiang M.; RT ; RL Submitted (11-OCT-2019) to the INSDC. RL College of Biological Science and Engineering, Beijing University of RL Agriculture, Changping District Huilongguanzhen Beinonglu 7, Beijing RL 102206, China XX DR MD5; dab9f175ec6e81277a3b86c80e2cd061. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..2451 FT /organism="synthetic construct" FT /mol_type="other RNA" FT /note="derived from Arabidopsis thaliana" FT /db_xref="taxon:32630" FT CDS 1..2445 FT /codon_start=1 FT /transl_table=11 FT /product="SUS1" FT /note="AtSUS1-opt; codon-optimized" FT /protein_id="QKZ93247.1" FT /translation="MANAERMITRVHSQRERLNETLVSERNEVLALLSRVEAKGKGILQ FT QNQIIAEFEALPEQTRKKLEGGPFFDLLKSTQEAIVLPPWVALAVRPRPGVWEYLRVNL FT HALVVEELQPAEFLHFKEELVDGVKNGNFTLELDFEPFNASIPRPTLHKYIGNGVDFLN FT RHLSAKLFHDKESLLPLLKFLRLHSHQGKNLMLSEKIQNLNTLQHTLRKAEEYLAELKS FT ETLYEEFEAKFEEIGLERGWGDNAERVLDMIRLLLDLLEAPDPCTLETFLGRVPMVFNV FT VILSPHGYFAQDNVLGYPDTGGQVVYILDQVRALEIEMLQRIKQQGLNIKPRILILTRL FT LPDAVGTTCGERLERVYDSEYCDILRVPFRTEKGIVRKWISRFEVWPYLETYTEDAAVE FT LSKELNGKPDLIIGNYSDGNLVASLLAHKLGVTQCTIAHALEKTKYPDSDIYWKKLDDK FT YHFSCQFTADIFAMNHTDFIITSTFQEIAGSKETVGQYESHTAFTLPGLYRVVHGIDVF FT DPKFNIVSPGADMSIYFPYTEEKRRLTKFHSEIEELLYSDVENKEHLCVLKDKKKPILF FT TMARLDRVKNLSGLVEWYGKNTRLRELANLVVVGGDRRKESKDNEEKAEMKKMYDLIEE FT YKLNGQFRWISSQMDRVRNGELYRYICDTKGAFVQPALYEAFGLTVVEAMTCGLPTFAT FT CKGGPAEIIVHGKSGFHIDPYHGDQAADTLADFFTKCKEDPSHWDEISKGGLQRIEEKY FT TWQIYSQRLLTLTGVYGFWKHVSNLDRLEARRYLEMFYALKYRPLAQAVPLAQDDGGGG FT GS" XX SQ Sequence 2451 BP; 615 A; 589 C; 628 G; 619 T; 0 other; atggcaaacg ctgaacgcat gatcactcgt gtacatagcc aacgtgaacg tctgaacgag 60 actctggtta gcgaacgtaa cgaggttctg gcactgctgt ctcgtgtaga agccaaaggc 120 aaaggcatcc tgcaacagaa ccagatcatt gctgagttcg aagctctgcc tgaacagact 180 cgcaagaaac tggaaggtgg tccgttcttc gatctgctga aatccactca agaggcaatc 240 gttctgccac cttgggtagc actggctgtt cgtccacgtc ctggtgtatg ggaatacctg 300 cgtgtcaatc tgcatgcact ggtagtcgaa gaactgcaac cagctgagtt tctgcacttc 360 aaagaagagc tggttgatgg cgttaagaac ggtaacttca ctctggaact ggacttcgaa 420 ccgttcaatg catccattcc gcgtccaact ctgcacaagt acatcggtaa cggtgtagac 480 ttcctgaacc gtcatctgtc tgctaaactg ttccatgaca aagagagcct gcttccactg 540 ttgaagttcc tgcgtctgca ctctcatcag ggcaagaacc tgatgctgtc tgagaagatc 600 cagaacctga acactctgca acacactctg cgcaaagctg aagagtacct cgcagaactg 660 aagtccgaga ctctgtacga agagttcgaa gccaagttcg aagagatcgg tctggaacgt 720 ggttggggtg acaatgcaga acgtgtactg gacatgattc gtctgctctt ggatctgctg 780 gaagcaccgg atccgtgtac tctggagacc tttctgggtc gtgtaccgat ggtattcaac 840 gttgtgatcc tgtctccgca tggctacttc gctcaagaca acgtactggg ttatccggat 900 actggtggtc aggttgtgta cattctggat caagtacgtg ctctggagat cgagatgctg 960 caacgcatca agcagcaagg tctgaacatc aaaccgcgta ttctgatcct gactcgtctt 1020 ctgccagatg cagtaggtac tacctgtggt gaacgtctcg aacgcgttta cgactctgag 1080 tactgtgaca ttctgcgtgt accgttccgt actgagaaag gcatcgttcg caaatggatc 1140 tctcgtttcg aagtatggcc atatctggag acttacaccg aagatgctgc agttgagctg 1200 tccaaagagc tgaatggcaa accggatctg atcattggca actacagcga tggcaatctg 1260 gtagcatctc tgcttgctca caaactgggt gtcactcagt gtaccattgc tcatgcactg 1320 gagaagacca agtatccgga ttctgacatc tactggaaga agctggatga caagtaccat 1380 ttcagctgtc agttcactgc tgatatcttc gcaatgaacc acactgactt catcatcact 1440 agcactttcc aagagatcgc tggtagcaaa gagactgtag gtcagtacga atctcacact 1500 gccttcactc tgccaggtct gtatcgtgtc gttcacggta ttgacgtgtt cgatccgaag 1560 ttcaacatcg tctctcctgg tgctgatatg agcatctact ttccgtacac cgaagagaag 1620 cgtcgtctga ctaagttcca ctctgagatc gaagagctgc tgtacagcga tgtcgagaac 1680 aaagagcatc tgtgtgtact gaaagacaag aagaagccga ttctgttcac catggcacgt 1740 ctggatcgtg tcaagaacct gtctggtctc gttgagtggt acggtaagaa cactcgtctg 1800 cgtgaactgg ctaatctggt agtcgttggt ggtgatcgtc gcaaagagtc caaagacaac 1860 gaagagaaag cagagatgaa gaagatgtac gatctgatcg aagagtacaa gctgaacggt 1920 cagttccgtt ggatctctag ccagatggat cgtgtacgta acggtgaact gtatcgctac 1980 atctgtgaca ccaaaggtgc attcgtccaa ccggctctgt atgaagcctt tggtctgact 2040 gttgtagagg ctatgacttg tggtcttccg actttcgcaa cttgcaaagg tggtccagct 2100 gagatcattg tgcacggtaa atctggtttc cacatcgatc cgtaccatgg tgatcaggca 2160 gctgatactc tggcagactt cttcaccaag tgcaaagaag atccgtctca ctgggatgag 2220 atcagcaaag gtggtctgca acgcatcgaa gagaagtaca cttggcaaat ctactctcag 2280 cgtctgttga ctctgactgg tgtgtatggc ttctggaagc atgtctctaa tctggatcgt 2340 cttgaagctc gtcgctacct ggagatgttc tacgcactga agtatcgtcc actggcacag 2400 gctgtaccac ttgcacaaga cgatggtggc ggcggtggat cctaatgata g 2451 // ID MN562656; SV 1; linear; other DNA; STD; SYN; 435 BP. XX AC MN562656; XX DT 10-JUL-2020 (Rel. 144, Created) DT 10-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct clone G6A anti-Hsp70 single chain Fv antibody gene, DE partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-435 RX PUBMED; 30998271. RA Vostakolaei M.A., Molavi O., Hejazi M.S., Kordi S., Rahmati S., RA Barzegari A., Abdolalizadeh J.; RT "Isolation and characterization of a novel scFv antibody fragments specific RT for Hsp70 as a tumor biomarker"; RL J. Cell. Biochem. 120(9):14711-14724(2019). XX RN [2] RP 1-435 RA Asghari Vostakolaei M.; RT ; RL Submitted (12-OCT-2019) to the INSDC. RL Pharmaceutical Biotechnology, Faculty of Pharmacy/Tabriz University of RL Medical Sciences, Daneshgah Street, Tabriz, East Azerbayjan 51664-14766, RL Iran XX DR MD5; 6cc95c2028c40a6a93a706b74d2303e8. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..435 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /country="Iran" FT /collected_by="Mehdi Asghari Vostakolaei" FT /collection_date="20-Nov-2018" FT /clone="G6A" FT /db_xref="taxon:32630" FT CDS <1..>435 FT /codon_start=1 FT /transl_table=11 FT /product="anti-Hsp70 single chain Fv antibody" FT /protein_id="QKZ93314.1" FT /translation="MAEVFDYWGQGTLVTVSSGGGGSGGGGSGGGGSTDIQMTQSPSSL FT SASVGDRVTITCRASQSISSYLNWYQQKPGKAPKLLIYAASSLQSGVPSRFSGSGSGTD FT FTLTISSLQPEDFATYYCQQSYSTPNTFGQGTKVEIKRAAA" XX SQ Sequence 435 BP; 105 A; 114 C; 125 G; 91 T; 0 other; atggccgagg tgtttgacta ctggggccag ggaaccctgg tcaccgtctc gagcggtgga 60 ggcggttcag gcggaggtgg cagcggcggt ggcgggtcga cggacatcca gatgacccag 120 tctccatcct ccctgtctgc atctgtagga gacagagtca ccatcacttg ccgggcaagt 180 cagagcatta gcagctattt aaattggtat cagcagaaac cagggaaagc ccctaagctc 240 ctgatctatg ctgcatccag tttgcaaagt ggggtcccat caaggttcag tggcagtgga 300 tctgggacag atttcactct caccatcagc agtctgcaac ctgaagattt tgcaacttac 360 tactgtcaac agagttacag tacccctaat acgttcggcc aagggaccaa ggtggaaatc 420 aaacgggcgg ccgca 435 // ID MN564743; SV 1; linear; other DNA; STD; SYN; 735 BP. XX AC MN564743; XX DT 10-JUL-2020 (Rel. 144, Created) DT 10-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct anti-VEGFR2 single chain Fv antibody gene, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RC Publication Status: Online-Only RP 1-735 RX PUBMED; 31522436. RA Kordi S., Rahmati-Yamchi M., Asghari Vostakolaei M., Etemadie A., RA Barzegari A., Abdolalizadeh J.; RT "Isolation of a Novel Anti-KDR3 Single-chain Variable Fragment Antibody RT from a Phage Display Library"; RL Iran J Allergy Asthma Immunol 18(3):289-299(2019). XX RN [2] RP 1-735 RA Kordi S., Asghari Vostakolaei M., Rahmati-Yamchi M., Etemadie A., RA Barzegari A., Abdolalizadeh J.; RT ; RL Submitted (12-OCT-2019) to the INSDC. RL Medical Biotechnology, Faculty of Advanced Medical Sciences/Tabriz RL University of Medical Sciences, Daneshgah Street, Tabriz, East Azerbayjan RL 51664-14766, Iran XX DR MD5; 3006f587242c2cc96e340f07e53d7658. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..735 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /country="Iran" FT /collected_by="Shirafkan Kordi" FT /collection_date="08-Jun-2019" FT /clone="A9K" FT /db_xref="taxon:32630" FT CDS <1..>735 FT /codon_start=1 FT /transl_table=11 FT /product="anti-VEGFR2 single chain Fv antibody" FT /protein_id="QKZ93353.1" FT /translation="MAEVQLLESGGGLVQPGGSLRLSCAASGFTFSSYAMSWVRQAPGK FT GLEWVSYIAANGADTSYADSVKGRFTISRDNSKNTLYLQMNSLRAEDTAVYYCAKAYSN FT FDYWDQGTLVTVSSGGGGSGGGGSGGGGSTDIQMTQSPSSLSASVGDRVTITCRASQSI FT SSYLNWYQQKPGKAPKLLIYGASNLQSGVPSRFSGSGSGTDFTLTISSLQPEDFATYYC FT QQGSSYPSTFGQGTKVEIKRAAA" XX SQ Sequence 735 BP; 170 A; 186 C; 214 G; 165 T; 0 other; atggccgagg tgcagctgtt ggagtctggg ggaggcttgg tacagcctgg ggggtccctg 60 agactctcct gtgcagcctc tggattcacc tttagcagct atgccatgag ctgggtccgc 120 caggctccag ggaaggggct ggagtgggtc tcatatattg ctgctaatgg tgctgataca 180 tcttacgcag actccgtgaa gggccggttc accatctcca gagacaattc caagaacacg 240 ctgtatctgc aaatgaacag cctgagagcc gaggacacgg ccgtatatta ctgtgcgaaa 300 gcttatagta attttgacta ctgggaccag ggaaccctgg tcaccgtctc gagcggtgga 360 ggcggttcag gcggaggtgg cagcggcggt ggcgggtcga cggacatcca gatgacccag 420 tctccatcct ccctgtctgc atctgtagga gacagagtca ccatcacttg ccgggcaagt 480 cagagcatta gcagctattt aaattggtat cagcagaaac cagggaaagc ccctaagctc 540 ctgatctatg gtgcatccaa tttgcaaagt ggggtcccat caaggttcag tggcagtgga 600 tctgggacag atttcactct caccatcagc agtctgcaac ctgaagattt tgcaacttac 660 tactgtcaac agggtagttc ttatccttct acgttcggcc aagggaccaa ggtggaaatc 720 aaacgggcgg ccgca 735 // ID MN533977; SV 1; linear; other RNA; STD; SYN; 813 BP. XX AC MN533977; XX DT 30-JUN-2020 (Rel. 144, Created) DT 30-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct CTLT gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-813 RA Yu L., Pan J., Cao G., Jiang M., Zhang Y., Zhu M., Liang Z., Zhang X., RA Hu X., Xue R., Gong C.; RT ; RL Submitted (02-OCT-2019) to the INSDC. RL Soochow University, School of Basic Medicine and Biological Sciences, 199 RL Renai Road, Suzhou, Jiangsu 215000, China XX DR MD5; a825ab6483e51372ee18a3b061ae119b. XX FH Key Location/Qualifiers FH FT source 1..813 FT /organism="synthetic construct" FT /mol_type="other RNA" FT /db_xref="taxon:32630" FT CDS 1..813 FT /codon_start=1 FT /transl_table=11 FT /product="CTLT" FT /note="codon optimized for Bombyx mori nucleopolyhedrovirus FT expression" FT /protein_id="QKW90293.1" FT /translation="MGMQVQIQSLFLLLLWVPGSRGGILGFVFTLAAYILGFVFTLTVK FT AAAKFVAAWTLKAAAKAACVNGSCFTVKAAAQIAILITTVTAAYITGFAPFSKAAASLC FT PIRGWAIGAAASIIPSGPLKAAAIMDKNIILKAAATSQYIKANSKFIGITEAAACLLKG FT IAPLNAAAELREQLSSVKAAAVTAACSHAGKAAATLTENNVPVNAAAYIVERPSAVKAA FT ANVSYSGTSKAAATLTERGVEVKAAAKLYGSGSKLKAAATVERTNIPR" XX SQ Sequence 813 BP; 160 A; 259 C; 210 G; 184 T; 0 other; atgggtatgc aagttcaaat ccaatcgttg ttcctcctgc tcctgtgggt gccgggtagc 60 cgtggcggta tcctgggttt cgtcttcacc ctcgctgcct acatcttggg cttcgtcttc 120 acactcaccg ttaaggctgc cgctaagttc gtcgccgctt ggaccttgaa ggccgctgcc 180 aaggctgcct gcgttaacgg ttcctgtttc actgtgaagg ctgccgctca gatcgccatc 240 ctgatcacca ctgtgacagc cgcttacatc accggcttcg ctccattctc taaggctgct 300 gccagcctct gcccaatcag gggttgggct atcggagctg ctgcttcgat catcccatcc 360 ggacctctga aggccgctgc catcatggac aagaacatca tcctcaaggc tgccgctact 420 tcacagtaca tcaaggccaa ctcgaagttc atcggaatca cagaggccgc tgcctgcctg 480 ctcaagggta tcgctccact gaacgctgcc gctgagttgc gtgaacaact gtcctctgtg 540 aaggccgctg ccgtcacagc tgcctgttct cacgctggca aggctgccgc tactctgaca 600 gagaacaacg tgcccgtcaa cgccgctgcc tacatcgttg aacgtcctag cgctgtgaag 660 gctgccgcta acgtcagcta ctcaggcact tccaaggccg ctgccacctt gactgagcgc 720 ggagttgaag tgaaggctgc cgctaagctg tacggctcag gcagcaagtt gaaggccgcc 780 gctacagttg aaagaacaaa catcccgaga taa 813 // ID MN533978; SV 1; linear; other RNA; STD; SYN; 1002 BP. XX AC MN533978; XX DT 30-JUN-2020 (Rel. 144, Created) DT 30-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct THB gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1002 RA Yu L., Pan J., Cao G., Jiang M., Zhang Y., Zhu M., Liang Z., Zhang X., RA Hu X., Xue R., Gong C.; RT ; RL Submitted (02-OCT-2019) to the INSDC. RL Soochow University, School of Basic Medicine and Biological Sciences, 199 RL Renai Road, Suzhou, Jiangsu 215000, China XX DR MD5; 8b013c3b18e0917980a2aec2f16c3ec6. XX FH Key Location/Qualifiers FH FT source 1..1002 FT /organism="synthetic construct" FT /mol_type="other RNA" FT /db_xref="taxon:32630" FT CDS 1..1002 FT /codon_start=1 FT /transl_table=11 FT /product="THB" FT /note="codon optimized for Bombyx mori nucleopolyhedrovirus FT expression" FT /protein_id="QKW90294.1" FT /translation="MGMQVQIQSLFLLLLWVPGSRGKRWIILGLNKIVRMYGPGPGRAL FT YHTENAYVSVVSSHYGPGPGKESWSYIVETPNPENGPGPGNHTVTGVSASCSHNGKKGP FT GPGSGPLKAEIAQRLEGPGPGPLKAEIAQRLEDVKKGPGPGEYLNKIQNSLSTEWSPCS FT VTGPGPGNVSYSGTSKACSDSFYRSGPGPGGGKWSYIVERPSAVNGMCGPGPGHNGMXX FT XTNLXHPLILNTKKGPGPGDPALIIWGIHHSGSTAEGPGPGSMGIQSDVQVDANCEGPG FT PGQTKLYGSGSKLITVGGPGPGAKFVAAWTLKAAAGGGGSGGGGSGGGGSID" XX SQ Sequence 1002 BP; 216 A; 282 C; 292 G; 205 T; 7 other; atgggtatgc aggttcaaat ccagtcgttg ttcttgctcc tcttgtgggt gccaggttct 60 aggggaaagc gttggatcat cttgggcctg aacaagatcg tgcgtatgta cggaccaggt 120 cctggaagag ctctctacca cacagagaac gcctacgtct ctgtggtctc ctctcactac 180 ggaccaggtc cgggcaagga aagctggtca tacatcgtgg agacccccaa ccctgaaaac 240 ggtcctggtc cgggcaacca caccgtcact ggcgtttcgg cttcctgctc tcacaacggc 300 aagaagggtc caggtcctgg ctccggacct ttgaaggctg agatcgctca gaggctggaa 360 ggccctggac caggaccact caaggctgag atcgctcaaa gattggaaga cgtcaagaag 420 ggtcctggcc caggagagta cctcaacaag atccagaaca gcttgtcaac agaatggagc 480 ccttgctcag ttaccggtcc aggaccagga aacgtgtcgt actccggcac ctcaaaggcc 540 tgttctgata gcttctaccg ttccggccct ggaccaggtg gtggaaagtg gtcttacatc 600 gtggagaggc caagcgctgt caacggcatg tgcggaccgg gtcccggcca caacggaatg 660 tthththcca ctaaccthhh tcacccacth atcctcaaca caaagaaggg acctggtcca 720 ggcgaccccg ctctgatcat ctggggtatc caccactcag gctcgactgc tgagggacca 780 ggtccaggct cgatgggcat ccagtccgac gttcaagtgg atgctaactg tgaaggacct 840 ggtccaggcc aaaccaagct gtacggttcc ggttctaagc tcatcactgt cggtggacca 900 ggacctggtg ctaagttcgt tgctgcctgg acactgaagg ccgctgctgg tggcggcggt 960 tcaggcggtg gtggttcggg aggcggcggc tctatcgact aa 1002 // ID MN756606; SV 1; circular; other DNA; STD; SYN; 10194 BP. XX AC MN756606; XX DT 30-JUN-2020 (Rel. 144, Created) DT 30-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct clone p47832mc, complete sequence. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Hepatitis E virus OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; OC Hepelivirales; Hepeviridae; Orthohepevirus; Orthohepevirus A. XX RN [1] RC Publication Status: Online-Only RP 1-10194 RA Scholz J., Bachlein C., Gadicherla A.K., Falkenhagen A., Tausch S.H., RA Johne R.; RT "Establishment of a Plasmid-Based Reverse Genetics System for the Cell RT Culture-Adapted Hepatitis E Virus Genotype 3c Strain 47832c"; RL Pathogens 9(3):-E157(2020). XX RN [2] RP 1-10194 RA Johne R., Falkenhagen A., Baechlein C., Scholz J., Tausch S.; RT ; RL Submitted (29-NOV-2019) to the INSDC. RL Unit 46, Federal institute for risk assessment, Diedersdorfer Weg 1, RL Berlin, Berlin 12277, Germany XX DR MD5; ad8f45346a3dafb14d62c9a643528259. XX CC ##Assembly-Data-START## CC Assembly Method :: spades v. 3.13.1 CC Sequencing Technology :: Illumina CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..10194 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /clone="p47832mc" FT /db_xref="taxon:32630" FT source 480..7912 FT /organism="Hepatitis E virus" FT /strain="47832c" FT /mol_type="other DNA" FT /db_xref="taxon:12461" FT regulatory 463..479 FT /note="T7 promotor" FT /regulatory_class="promoter" FT 5'UTR 480..504 FT CDS 505..5802 FT /codon_start=1 FT /transl_table=11 FT /product="nonstrucural polyprotein" FT /note="ORF1" FT /protein_id="QKX95862.1" FT /translation="MEAHQFIKAPGITTAIEQAALAAANSALANAVVVRPFLSRVQTEI FT LINLMQPRQLVFRPEVLWNHPIQRVIHNELEQYCRARAGRCLEVGAHPRSINDNPNVLH FT RCFLRPVGRDVQRWYSAPTRGPAANCRRSALRGLPPADRTYCFDGFSRCSFAAETGVAL FT YSLHDLWPADVAEAMARHGMTRLYAALHLPPEVLLPPGTYHTTSYLLIHDGDRAVVTYE FT GDTSAGYNHDVSILRAWIRTTKIVGDHPLVIERVRAIGCHFVLLLTAAPEPSPMPYVPY FT PRSTEVYVRSIFGPGGSPSLFPSACSTKSTFHAVPVHIWDRLMLFGATLDDQAFCCSRL FT MTYLRGISYKVTVGALVANEGWNASEDALTAVITAAYLTICHQRYLRTQAISKGMRRLE FT VEHAQKFITRLYSWLFEKSGRDYIPGRQLQFYAQCRRWLSAGFHLDPRVLVFDESVPCR FT CRTFLKKAVGKFCCFMKWLGQECTCFLEPAEGLVGDHGHDNEAYEGSEVDQAEPAHLDV FT SGTYAVHGRQLEALYRALNIPHDIAARASRLTATVELVAGPDRLECRTVLGNKTFRTTV FT VDGAHLEANGPEQYVLSFDASRQSMGAGPHSLSYELTPAGLQVKISSNGLDCTATFPPG FT GAPSAAPEEVAAFCSALYRYNRFTQRHSLIGGLWLHPEGLLGIFPPFSPGHLWESANPF FT CGEGTLYTRTWSTSGFSSDFSPPEAAAAAPAAAPGLRHPKPPVSDIWVLPPSSEEFQVG FT TAPAPPAPAPAQPSNPAGPKAPVRKLPMLRGLTNVAQVCVDVVSRVCGVSPGPPSSEEF FT QVGTAPAPPAPAPAQPSNPAGPKAPVRKLPMPPSPRTRRLLYTYPDGAKVYVGSLFESD FT CDWLVNASNPGHRPGGGLCHAFYQRYPESFHPTEFIMRDGLAAYTLTPRPIIHAVAPDY FT RVEHNPKRLEAAYRETCSRRGTAAYPLLGSGIYQVPVSLSFDAWERNHRPGDELYLTDL FT AATWFEANKPTQPTLTITEDAARTANLALEIDAATEVGRACAGCAVSPGVVHYQFTAGV FT PGSGKSRSIQQGDVDVVVVPTRELRNSWRRRGFAAFTPHTAARVTTGRRVVIDEAPSLP FT PHLLLLHMQRASSVHLLGDPNQIPAIDFEHAGLVPAIRPELAPTSWWHVTHRCPADVCE FT LIRGAYPKIQTTSRVLRSLFWNEPAIGQKLVFTQAAKAANPGAITVHEAQGATFTETTI FT IATADARGLIQSSRAHAIVALTRHTEKCVILDAPGLLREVGISDVIVNNFFLAGGEVGH FT HRPSVIPRGYPDQNLATLQAFPPSCQISAYHQLAEELGHRPAPVAAVLPPCPELEQGLL FT YMPQELTVSDSVLVFELTDIVHCRMAAPSQRKAVLSTLVGRYGRRTKLYEAAHSDVRES FT LARFIPTIGPVQATTCELYELVEAMVEKGQDGSAVLELDLCNRDVSRITFFQKDCNKFT FT TGETIAHGKVGQGISAWSKTFCALFGPWFRAIEKEILALLPPNIFYGDAYEESVFAAAV FT SGAGSSMVFENDFSEFDSTQNNFSLGLECVVMEECGMPQWLIRLYHLVRSAWILQAPKE FT SLKGFWKKHSGEPGTLLWNTVWNMAIIAHCYEFRDFRVAAFKGDDSVVLCSDYRQSRNA FT AALIAGCGLKLKVDYRPIGLYAGVVVAPGLGTLPDVVRFAGRLSEKNWGPGPERAEQLR FT LAVCDFLRGLTNVAQVCVDVVSRVYGVSPGLVHNLIGMLQTIADGKAHFTETVKPVLDL FT TNSIIQRVE" FT misc_feature 2733..2918 FT /note="insertion" FT CDS 5799..6167 FT /codon_start=1 FT /transl_table=11 FT /product="small phosphoprotein" FT /note="ORF3" FT /protein_id="QKX95864.1" FT /translation="MNNMFCALPMGSPCALGLFCCCSSCFCLCCPRHRPASRLAAVVGG FT AAAVPAVVSGVTGLILSPSPSPIFIQPTPLPPMSYRNPGLELALDSHPAPSAPLGAISP FT SAPLLSHVVDLPQPGLRR" FT CDS 5837..7819 FT /codon_start=1 FT /transl_table=11 FT /product="capsid protein" FT /note="ORF2" FT /protein_id="QKX95863.1" FT /translation="MRPRAVLLLFFMLLPMLPAPPAGQPSGRRRGRRSGGTGSGFWGDR FT VDSQPFALPYIHPTNPFAADVVPQSGARARPRQPPRPLGSSWRDQSQRPSAVPRRRSAP FT AGAAPLTAVSPAPDTAPVPDVDSRGAILRRQYNLSTSPLTSSVASGTNLVLYAAPLNPL FT LPLQDGTNTHIMATEASNYAQYRVVRATIRYRPLVPNAVGGYAISISFWPQTTTTPTSV FT DMNSITSTDVRILVQPGIASELVIPSERLHYRNQGWRSVETSGVAEEEATSGLVMLCIH FT GSPVNSYTNTPYTGALGLLDFALELEFRNLTPGNTNTRVSRYTSTARHRLRRGADGTAE FT LTTTAATRFMKDLHFTGTNGVGEVGRGIALTLFNLADTLLGGLPTELISSAGGQLFYSR FT PVVSANGEPTVKLYTSVENAQQDKGIAIPHDIDLGDSRVVIQDYDNQHEQDRPTPSPAP FT SRPFSVLRANDVLWLSLTAAEYDQTTYGSSTNPMYVSDTVTFVNVATGAQAVARSLDWS FT KVTLDGRPLTTIQQYSKTFYVLPLRGKLSFWEAGTTKAGYPYNYNTTASDQILIENAAG FT HRVAISTYTTSLGAGPVSVSAVGVLAPHSALAVLEDTIDYSARAHTFDDFCPECRALGL FT QGCAFQSTIAELQRLKMKVGKTREF" FT 3'UTR 7820..7887 FT misc_feature 7888..7909 FT /note="poly-A tail" FT rep_origin 8367..8955 FT gene 9126..9986 FT /gene="beta-lactamase" XX SQ Sequence 10194 BP; 2073 A; 2898 C; 2633 G; 2590 T; 0 other; tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 60 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 120 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc 180 gacgctctcc cttatgcgac tcctgcatta agaagcagcc cagtagtagg ttgaggccgt 240 tgagcaccgc cgccgcaagg aatggtgcaa ggagatggcg cccaacagtc ccccggccac 300 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg 360 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt 420 gatgccggcc acgatgcgtc cggcgtagag gatctggcta gctaatacga ctcactatag 480 cagaccacgt atgtggtcga tgccatggag gcccatcagt tcattaaggc tcctggcatt 540 actactgcca ttgagcaagc tgctctggct gcggccaatt ctgccctggc gaatgctgtg 600 gtggttcggc cgtttttatc tcgtgtgcaa actgagattc ttattaattt gatgcaaccc 660 cggcagttgg tttttcgacc tgaagtgctc tggaatcatc ccatccaacg ggttatccat 720 aatgaacttg aacagtactg tcgggcccgg gctggtcgtt gtctagaggt tggggcccac 780 ccaagatcca ttaatgacaa cccaaatgtt ctgcaccggt gttttctacg accagttggg 840 agagatgttc agcgctggta ctctgctcct acccgcggcc ctgcggctaa ctgccgccgt 900 tctgccttgc gtggcctccc ccctgctgat cgtacctatt gttttgatgg attctcccgc 960 tgctcatttg ctgcagaaac tggggttgcc ctttattctc tgcatgacct ctggccggcc 1020 gatgttgcgg aggctatggc ccgacatggt atgacacgct tgtatgctgc actacatctc 1080 ccccctgaag tgctactacc acccggtact taccatacaa cttcatacct tctgattcac 1140 gacggagatc gtgctgttgt gacctatgaa ggcgatacta gtgcaggcta caatcatgat 1200 gtttccatac tccgtgcatg gatccgcaca actaagatag tcggcgatca tccgctggtg 1260 atagagcgtg tgcgggctat tggctgtcat tttgtgctgc tgcttactgc agcccctgag 1320 ccgtcaccaa tgccttacgt tccatacccc cggtcgacag aggtgtatgt ccgctctata 1380 ttcggccctg gcgggtcccc atccctattc ccatcagctt gctctacgaa atccacattt 1440 cacgctgtcc cggttcatat ttgggaccgg ctcatgcttt ttggtgctac tctggatgat 1500 caggcgtttt gctgttcacg gcttatgacc tacctccgcg ggattagtta caaggtcacc 1560 gttggcgccc ttgtcgctaa tgaggggtgg aatgcctcgg aggacgctct taccgctgtt 1620 attactgcag cgtatttgac catttgccat cagcgttacc tccgtactca agctatatcc 1680 aagggtatgc gtcgactgga ggttgagcat gcccaaaaat tcattacaag actttatagt 1740 tggctgttcg agaagtccgg ccgcgactat atccccggcc gccagctcca gttttatgca 1800 cagtgccgcc gctggttatc ggcaggtttt catcttgacc caagggtgct tgtttttgat 1860 gagtctgtac cctgccgttg taggacattt cttaagaagg ctgtgggtaa gttctgctgt 1920 tttatgaagt ggttgggaca ggagtgcacc tgctttttgg aaccagcaga gggtctagtt 1980 ggcgaccatg gccacgataa tgaagcttat gaaggctctg aggttgatca ggccgagccc 2040 gcccatctcg atgtttctgg gacctatgcc gtccatggcc gtcaacttga ggccctgtat 2100 agagcgctta acatcccgca tgacatcgct gcccgagcct cccgtttgac tgccaccgtc 2160 gaactcgttg caggtccaga ccgtttggag tgccgcactg tgctcgggaa taagactttc 2220 cggacgacgg tggttgacgg cgcccatctt gaggcgaatg gccccgagca gtacgttctt 2280 tcgtttgatg cctctcgcca gtccatgggg gccgggccgc atagcctctc ctacgagctc 2340 actcctgctg gtctgcaggt caagatttca tctaatgggc tggattgcac tgcaacattc 2400 cccccgggcg gggcccctag tgctgctccg gaggaggtag cagccttttg cagtgctctt 2460 tacaggtata acaggttcac tcagcgccac tcacttatag gtggcctgtg gctgcatccc 2520 gaggggttgt tgggtatttt cccccctttc tctcctgggc acctctggga gtctgctaac 2580 cctttttgtg gggagggaac cttgtacacc cggacatggt caacatctgg tttttctagt 2640 gacttctccc cccctgaggc agccgccgca gcgccggctg ctgccccggg gctacgccac 2700 cctaaacctc ctgttagtga catttgggtg ttaccgccat cttctgaaga atttcaggtt 2760 ggcacagcgc ccgctccccc tgcccctgcg cccgctcaac catccaaccc cgccgggcca 2820 aaggcccccg tgcgtaagct gccaatgctt cgagggttaa cgaatgttgc gcaggtatgt 2880 gttgatgttg tatcccgagt ttgcggagtt agccctggac cgccatcttc tgaagaattt 2940 caggttggca cagcgcccgc tccccctgcc cctgcgcccg ctcaaccatc caaccccgcc 3000 gggccaaagg cccccgtgcg taagctgcca atgccaccat ccccgcgcac ccgccgcctt 3060 ctttacacct atccggatgg ggcaaaggtg tatgtagggt cactgtttga gtctgactgt 3120 gattggctgg ttaatgcgtc gaatcccggc catcgccctg gaggcggcct ttgccatgcc 3180 ttctaccaac gctaccccga gtctttccac ccaactgagt tcattatgcg cgacggtctt 3240 gccgcgtata ctttaactcc ccggcctatt attcatgcag tggctcctga ctatagggtt 3300 gagcataacc ccaagaggct tgaggcggca taccgagaga cttgctctcg ccgcggtacc 3360 gccgcctacc cactcctcgg ctcgggtata taccaagttc ccgtcagcct cagctttgac 3420 gcttgggagc gtaaccaccg ccccggggat gagctctacc taaccgacct cgccgctacc 3480 tggttcgagg ctaataagcc aacgcagccg accctcacaa taactgagga tgcggcccgt 3540 acagccaacc tagcactgga gatcgatgct gctacggagg tcggccgggc ttgtgccggc 3600 tgcgcagtta gtcctggggt tgtgcactat cagtttactg ctggggtccc aggttcgggg 3660 aagtcgcgct ctatacagca gggggatgtt gatgtggtgg ttgttcccac tcgggagctc 3720 cggaatagtt ggcgtcgccg gggtttcgcg gcttttacac ctcatacggc ggcccgtgtc 3780 accacgggcc gtcgtgttgt gattgatgag gccccatccc tcccaccgca tttgttgcta 3840 ctacacatgc agcgggcctc gtcggtccac cttctcggcg acccaaacca gatccctgcc 3900 atagacttcg agcatgccgg cctggtcccc gcaatacgtc ctgagcttgc gcccaccagt 3960 tggtggcatg tcacccatcg ctgccccgct gacgtgtgcg agcttatacg cggggcctat 4020 cccaaaatcc aaaccactag ccgcgtgctg cggtctttat tctggaatga gcctgccatc 4080 ggccagaagt tagtttttac tcaggctgct aaggctgcca accccggtgc gattacagtc 4140 cacgaggccc agggcgctac tttcacggag actacaatca tagccacggc tgatgctagg 4200 gggctcatcc aatcttcccg agcccatgcc atagtcgcac tcacccgcca cacagagaag 4260 tgcgttattc ttgatgcccc cggcttgtta cgtgaggtcg gtatatcgga tgtgattgtc 4320 aacaattttt tcctcgccgg cggggaggtg ggtcaccatc gcccctctgt gataccccgc 4380 ggctatcctg accagaacct cgcgacacta caggcctttc caccttcctg ccagattagt 4440 gcctaccacc agctagctga ggaacttggc caccgcccag ctcccgtcgc cgctgtcttg 4500 cccccttgcc ctgaacttga gcagggcttg ttatatatgc ctcaggagct tacggtgtct 4560 gatagcgtgc tagtctttga actcacggac atagtccact gccggatggc cgcccccagc 4620 cagcggaagg ccgtcctatc gacgctcgtg ggtaggtatg gccgtcggac gaagctgtat 4680 gaagcagctc actctgacgt ccgtgagtcc ctggctagat tcatccccac cattgggcct 4740 gttcaggcta ccacgtgtga gttatatgag ctggttgagg ccatggtgga gaaaggtcag 4800 gatggctctg ccgtgcttga gctcgacctt tgcaatcgtg atgtatcgcg tatcacattt 4860 ttccagaaag attgtaacaa attcaccaca ggggagacca ttgcccacgg taaggttggc 4920 cagggcatct cggcttggag taagaccttt tgtgccctgt ttggtccgtg gtttcgtgct 4980 attgaaaaag aaatactagc cctgctccca cctaatattt tctacggcga cgcatacgag 5040 gagtctgtgt ttgccgccgc tgtgtcgggg gcaggttcaa gcatggtatt tgagaatgat 5100 ttctcagagt ttgatagcac ccaaaataac ttctcccttg gtctcgagtg tgtagtcatg 5160 gaggaatgtg gcatgcccca gtggctaatc cggttgtacc atttggtccg gtcggcctgg 5220 atcctacagg cgccgaagga gtctctcaag ggattttgga agaagcattc tggtgagccc 5280 ggcaccctcc tctggaacac tgtttggaat atggcgatca tagcacattg ctatgaattt 5340 cgtgatttta gagttgccgc tttcaaggga gatgattccg tggtcctctg tagcgactac 5400 cgtcagagcc gcaatgcagc ggccctgatt gcaggttgcg ggctcaaact gaaggttgat 5460 tatcgcccca ttgggttgta tgctggtgtg gtggtggccc ctggcctagg gacgctaccc 5520 gatgtggtgc gctttgccgg ccggctgtct gagaagaact ggggccctgg gccggagcgg 5580 gctgagcagt tgcgcctagc tgtttgtgac tttcttcgag ggttaacgaa tgttgcgcag 5640 gtatgtgttg atgttgtatc ccgagtttac ggggttagcc ctgggctggt acataacctt 5700 attggcatgt tgcaaaccat agctgatggc aaagcccatt ttacagagac tgtcaaacct 5760 gtgcttgacc tcacgaactc tatcatacag cgggtggaat gaataacatg ttttgtgcat 5820 tgcccatggg atcaccatgc gccctagggc tgttctgttg ctgttcttca tgcttctgcc 5880 tatgctgccc gcgccaccgg ccggccagcc gtctggccgc cgtcgtgggc ggcgcagcgg 5940 cggtaccggc agtggtttct ggggtgacag ggttgattct cagcccttcg ccctccccta 6000 tattcatcca accaacccct ttgccgccga tgtcgtaccg caatccgggg ctagagctcg 6060 ccctcgacag ccaccccgcc ccctcggctc ctcttggcgc gatcagtccc agcgcccctc 6120 tgctgtccca cgtcgtcgat ctgccccagc cggggctgcg ccgctgactg ccgtttcacc 6180 tgctcccgac acagctcctg tacctgatgt tgactcgcgc ggcgccatac tgcgacgcca 6240 gtacaattta tccacatccc cgctcacatc atctgttgct tcgggcacta atctggttct 6300 ttatgccgcc ccgctaaacc ctttgctgcc ccttcaggat ggcactaaca ctcatatcat 6360 ggccactgag gcatctaatt atgcccagta tcgggttgtc cgagctacga tccgttacag 6420 gccattggtg ccaaatgctg tcggcggcta tgcaatatcc atctcattct ggcctcagac 6480 tactactacc cccacgtctg ttgatatgaa ctctattact tccactgatg ttaggattct 6540 agttcaaccc ggcattgcct ctgagttggt cattcctagt gagcgcctcc attatcgtaa 6600 ccagggctgg cgctctgtgg agacctctgg cgtggctgaa gaggaggcta cttctggttt 6660 ggtaatgctt tgtatccatg gctctcctgt taattcctac accaataccc cctataccgg 6720 ggcgcttgga ctccttgatt tcgctttaga gcttgagttt aggaatttga cacccgggaa 6780 caccaacact cgtgtgtccc ggtatacaag cacagcccgt catcggctgc gccgcggtgc 6840 tgatggcacc gccgaactta ccaccacagc ggccacgcgc tttatgaagg acctgcactt 6900 caccggtacg aatggggtcg gtgaggtggg tcgtggtatt gctctcacac tctttaatct 6960 tgctgacacg cttctcggtg gtttgccgac agaattaatt tcgtcggccg ggggacagtt 7020 attctactcc cgccccgtcg tctcagccaa tggcgagccg actgttaagt tatatacatc 7080 tgtagagaat gcgcagcagg ataaagggat cgctatccca catgacatag atctgggtga 7140 ctcccgtgtg gtcatccaag actatgacaa ccagcatgag caggatcgac ccaccccctc 7200 gcctgcccct tctcgccctt tttcggttct tcgcgctaat gatgtcctat ggctttctct 7260 tactgccgct gagtacgacc agactacata tgggtcgtcc actaacccga tgtatgtctc 7320 ggatactgtc acatttgtca atgtggctac aggcgcccag gctgtcgccc gttccctcga 7380 ctggtctaaa gttactctgg acggccgtcc tcttactact atccagcagt actccaaaac 7440 attttatgtt ctcccgctcc gcgggaagtt atctttttgg gaggccggga caactaaggc 7500 cggttacccc tataattata acacaactgc tagtgatcag attctgatcg aaaatgcggc 7560 cggtcatcgt gttgctattt ccacgtatac caccagcttg ggcgctggcc ctgtgtctgt 7620 ttctgcagtc ggtgttttag ccccacattc ggctcttgca gtccttgaag acactattga 7680 ctactctgcc cgtgcccaca cttttgatga tttctgcccg gagtgtcgcg ctcttggttt 7740 gcaggggtgt gccttccagt ctactattgc tgagcttcag cgtcttaaaa tgaaggtagg 7800 taaaacccgg gagttttaat caatttcctt tgtgccccct tcatagcttt tgctttattt 7860 cttttttctg cgtttcgcgc tcccaggaaa aaaaaaaaaa aaaaaaaaaa tttaaatatc 7920 agttctggac cagcgagctg tgctgcgact cgtggcgtaa tcatggtcat agctgtttcc 7980 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 8040 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 8100 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 8160 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 8220 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 8280 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 8340 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 8400 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 8460 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 8520 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 8580 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 8640 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 8700 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 8760 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 8820 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 8880 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 8940 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 9000 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 9060 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 9120 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 9180 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 9240 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 9300 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 9360 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 9420 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 9480 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 9540 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 9600 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 9660 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 9720 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 9780 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 9840 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 9900 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 9960 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 10020 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 10080 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 10140 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcg 10194 // ID MN756607; SV 1; circular; other DNA; STD; SYN; 10194 BP. XX AC MN756607; XX DT 30-JUN-2020 (Rel. 144, Created) DT 30-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct clone p47832mc/EcoRI, complete sequence. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX OS Hepatitis E virus OC Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; OC Hepelivirales; Hepeviridae; Orthohepevirus; Orthohepevirus A. XX RN [1] RC Publication Status: Online-Only RP 1-10194 RA Scholz J., Bachlein C., Gadicherla A.K., Falkenhagen A., Tausch S.H., RA Johne R.; RT "Establishment of a Plasmid-Based Reverse Genetics System for the Cell RT Culture-Adapted Hepatitis E Virus Genotype 3c Strain 47832c"; RL Pathogens 9(3):-E157(2020). XX RN [2] RP 1-10194 RA Johne R., Falkenhagen A., Baechlein C., Scholz J., Tausch S.; RT ; RL Submitted (29-NOV-2019) to the INSDC. RL Unit 46, Federal institute for risk assessment, Diedersdorfer Weg 1, RL Berlin, Berlin 12277, Germany XX DR MD5; a32c0b0efde107072d9a1dc1efd4078e. XX CC ##Assembly-Data-START## CC Assembly Method :: spades v. 3.13.1 CC Sequencing Technology :: Illumina CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..10194 FT /organism="synthetic construct" FT /focus FT /mol_type="other DNA" FT /clone="p47832mc/EcoRI" FT /db_xref="taxon:32630" FT source 480..7887 FT /organism="Hepatitis E virus" FT /strain="47832c" FT /mol_type="other DNA" FT /db_xref="taxon:12461" FT regulatory 463..479 FT /note="T7 promotor" FT /regulatory_class="promoter" FT 5'UTR 480..504 FT CDS 505..5802 FT /codon_start=1 FT /transl_table=11 FT /product="nonstrucural polyprotein" FT /note="ORF1" FT /protein_id="QKX95865.1" FT /translation="MEAHQFIKAPGITTAIEQAALAAANSALANAVVVRPFLSRVQTEI FT LINLMQPRQLVFRPEVLWNHPIQRVIHNELEQYCRARAGRCLEVGAHPRSINDNPNVLH FT RCFLRPVGRDVQRWYSAPTRGPAANCRRSALRGLPPADRTYCFDGFSRCSFAAETGVAL FT YSLHDLWPADVAEAMARHGMTRLYAALHLPPEVLLPPGTYHTTSYLLIHDGDRAVVTYE FT GDTSAGYNHDVSILRAWIRTTKIVGDHPLVIERVRAIGCHFVLLLTAAPEPSPMPYVPY FT PRSTEVYVRSIFGPGGSPSLFPSACSTKSTFHAVPVHIWDRLMLFGATLDDQAFCCSRL FT MTYLRGISYKVTVGALVANEGWNASEDALTAVITAAYLTICHQRYLRTQAISKGMRRLE FT VEHAQKFITRLYSWLFEKSGRDYIPGRQLQFYAQCRRWLSAGFHLDPRVLVFDESVPCR FT CRTFLKKAVGKFCCFMKWLGQECTCFLEPAEGLVGDHGHDNEAYEGSEVDQAEPAHLDV FT SGTYAVHGRQLEALYRALNIPHDIAARASRLTATVELVAGPDRLECRTVLGNKTFRTTV FT VDGAHLEANGPEQYVLSFDASRQSMGAGPHSLSYELTPAGLQVKISSNGLDCTATFPPG FT GAPSAAPEEVAAFCSALYRYNRFTQRHSLIGGLWLHPEGLLGIFPPFSPGHLWESANPF FT CGEGTLYTRTWSTSGFSSDFSPPEAAAAAPAAAPGLRHPKPPVSDIWVLPPSSEEFQVG FT TAPAPPAPAPAQPSNPAGPKAPVRKLPMLRGLTNVAQVCVDVVSRVCGVSPGPPSSEEF FT QVGTAPAPPAPAPAQPSNPAGPKAPVRKLPMPPSPRTRRLLYTYPDGAKVYVGSLFESD FT CDWLVNASNPGHRPGGGLCHAFYQRYPESFHPTEFIMRDGLAAYTLTPRPIIHAVAPDY FT RVEHNPKRLEAAYRETCSRRGTAAYPLLGSGIYQVPVSLSFDAWERNHRPGDELYLTDL FT AATWFEANKPTQPTLTITEDAARTANLALEIDAATEVGRACAGCAVSPGVVHYQFTAGV FT PGSGKSRSIQQGDVDVVVVPTRELRNSWRRRGFAAFTPHTAARVTTGRRVVIDEAPSLP FT PHLLLLHMQRASSVHLLGDPNQIPAIDFEHAGLVPAIRPELAPTSWWHVTHRCPADVCE FT LIRGAYPKIQTTSRVLRSLFWNEPAIGQKLVFTQAAKAANPGAITVHEAQGATFTETTI FT IATADARGLIQSSRAHAIVALTRHTEKCVILDAPGLLREVGISDVIVNNFFLAGGEVGH FT HRPSVIPRGYPDQNLATLQAFPPSCQISAYHQLAEELGHRPAPVAAVLPPCPELEQGLL FT YMPQELTVSDSVLVFELTDIVHCRMAAPSQRKAVLSTLVGRYGRRTKLYEAAHSDVRES FT LARFIPTIGPVQATTCELYELVEAMVEKGQDGSAVLELDLCNRDVSRITFFQKDCNKFT FT TGETIAHGKVGQGISAWSKTFCALFGPWFRAIEKEILALLPPNIFYGDAYEESVFAAAV FT SGAGSSMVFENDFSEFDSTQNNFSLGLECVVMEECGMPQWLIRLYHLVRSAWILQAPKE FT SLKGFWKKHSGEPGTLLWNTVWNMAIIAHCYEFRDFRVAAFKGDDSVVLCSDYRQSRNA FT AALIAGCGLKLKVDYRPIGLYAGVVVAPGLGTLPDVVRFAGRLSEKNWGPGPERAEQLR FT LAVCDFLRGLTNVAQVCVDVVSRVYGVSPGLVHNLIGMLQTIADGKAHFTETVKPVLDL FT TNSIIQRVE" FT misc_feature 2733..2918 FT /note="insertion" FT misc_feature 2749..2754 FT /note="introduced EcoRI single restriction site" FT CDS 5799..6167 FT /codon_start=1 FT /transl_table=11 FT /product="small phosphoprotein" FT /note="ORF3" FT /protein_id="QKX95867.1" FT /translation="MNNMFCALPMGSPCALGLFCCCSSCFCLCCPRHRPASRLAAVVGG FT AAAVPAVVSGVTGLILSPSPSPIFIQPTPLPPMSYRNPGLELALDSHPAPSAPLGAISP FT SAPLLSHVVDLPQPGLRR" FT CDS 5837..7819 FT /codon_start=1 FT /transl_table=11 FT /product="capsid protein" FT /note="ORF2" FT /protein_id="QKX95866.1" FT /translation="MRPRAVLLLFFMLLPMLPAPPAGQPSGRRRGRRSGGTGSGFWGDR FT VDSQPFALPYIHPTNPFAADVVPQSGARARPRQPPRPLGSSWRDQSQRPSAVPRRRSAP FT AGAAPLTAVSPAPDTAPVPDVDSRGAILRRQYNLSTSPLTSSVASGTNLVLYAAPLNPL FT LPLQDGTNTHIMATEASNYAQYRVVRATIRYRPLVPNAVGGYAISISFWPQTTTTPTSV FT DMNSITSTDVRILVQPGIASELVIPSERLHYRNQGWRSVETSGVAEEEATSGLVMLCIH FT GSPVNSYTNTPYTGALGLLDFALELEFRNLTPGNTNTRVSRYTSTARHRLRRGADGTAE FT LTTTAATRFMKDLHFTGTNGVGEVGRGIALTLFNLADTLLGGLPTELISSAGGQLFYSR FT PVVSANGEPTVKLYTSVENAQQDKGIAIPHDIDLGDSRVVIQDYDNQHEQDRPTPSPAP FT SRPFSVLRANDVLWLSLTAAEYDQTTYGSSTNPMYVSDTVTFVNVATGAQAVARSLDWS FT KVTLDGRPLTTIQQYSKTFYVLPLRGKLSFWEAGTTKAGYPYNYNTTASDQILIENAAG FT HRVAISTYTTSLGAGPVSVSAVGVLAPHSALAVLEDTIDYSARAHTFDDFCPECRALGL FT QGCAFQSTIAELQRLKMKVGKTREF" FT 3'UTR 7820..7887 FT misc_feature 7888..7909 FT /note="poly-A tail" FT rep_origin 8367..8955 FT /note="origin of replication" FT gene 9126..9986 FT /gene="beta-lactamase" XX SQ Sequence 10194 BP; 2073 A; 2899 C; 2633 G; 2589 T; 0 other; tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 60 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 120 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccattc 180 gacgctctcc cttatgcgac tcctgcatta agaagcagcc cagtagtagg ttgaggccgt 240 tgagcaccgc cgccgcaagg aatggtgcaa ggagatggcg cccaacagtc ccccggccac 300 ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg 360 atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt 420 gatgccggcc acgatgcgtc cggcgtagag gatctggcta gctaatacga ctcactatag 480 cagaccacgt atgtggtcga tgccatggag gcccatcagt tcattaaggc tcctggcatt 540 actactgcca ttgagcaagc tgctctggct gcggccaatt ctgccctggc gaatgctgtg 600 gtggttcggc cgtttttatc tcgtgtgcaa actgagattc ttattaattt gatgcaaccc 660 cggcagttgg tttttcgacc tgaagtgctc tggaatcatc ccatccaacg ggttatccat 720 aatgaacttg aacagtactg tcgggcccgg gctggtcgtt gtctagaggt tggggcccac 780 ccaagatcca ttaatgacaa cccaaatgtt ctgcaccggt gttttctacg accagttggg 840 agagatgttc agcgctggta ctctgctcct acccgcggcc ctgcggctaa ctgccgccgt 900 tctgccttgc gtggcctccc ccctgctgat cgtacctatt gttttgatgg attctcccgc 960 tgctcatttg ctgcagaaac tggggttgcc ctttattctc tgcatgacct ctggccggcc 1020 gatgttgcgg aggctatggc ccgacatggt atgacacgct tgtatgctgc actacatctc 1080 ccccctgaag tgctactacc acccggtact taccatacaa cttcatacct tctgattcac 1140 gacggagatc gtgctgttgt gacctatgaa ggcgatacta gtgcaggcta caatcatgat 1200 gtttccatac tccgtgcatg gatccgcaca actaagatag tcggcgatca tccgctggtg 1260 atagagcgtg tgcgggctat tggctgtcat tttgtgctgc tgcttactgc agcccctgag 1320 ccgtcaccaa tgccttacgt tccatacccc cggtcgacag aggtgtatgt ccgctctata 1380 ttcggccctg gcgggtcccc atccctattc ccatcagctt gctctacgaa atccacattt 1440 cacgctgtcc cggttcatat ttgggaccgg ctcatgcttt ttggtgctac tctggatgat 1500 caggcgtttt gctgttcacg gcttatgacc tacctccgcg ggattagtta caaggtcacc 1560 gttggcgccc ttgtcgctaa tgaggggtgg aatgcctcgg aggacgctct taccgctgtt 1620 attactgcag cgtatttgac catttgccat cagcgttacc tccgtactca agctatatcc 1680 aagggtatgc gtcgactgga ggttgagcat gcccaaaaat tcattacaag actttatagt 1740 tggctgttcg agaagtccgg ccgcgactat atccccggcc gccagctcca gttttatgca 1800 cagtgccgcc gctggttatc ggcaggtttt catcttgacc caagggtgct tgtttttgat 1860 gagtctgtac cctgccgttg taggacattt cttaagaagg ctgtgggtaa gttctgctgt 1920 tttatgaagt ggttgggaca ggagtgcacc tgctttttgg aaccagcaga gggtctagtt 1980 ggcgaccatg gccacgataa tgaagcttat gaaggctctg aggttgatca ggccgagccc 2040 gcccatctcg atgtttctgg gacctatgcc gtccatggcc gtcaacttga ggccctgtat 2100 agagcgctta acatcccgca tgacatcgct gcccgagcct cccgtttgac tgccaccgtc 2160 gaactcgttg caggtccaga ccgtttggag tgccgcactg tgctcgggaa taagactttc 2220 cggacgacgg tggttgacgg cgcccatctt gaggcgaatg gccccgagca gtacgttctt 2280 tcgtttgatg cctctcgcca gtccatgggg gccgggccgc atagcctctc ctacgagctc 2340 actcctgctg gtctgcaggt caagatttca tctaatgggc tggattgcac tgcaacattc 2400 cccccgggcg gggcccctag tgctgctccg gaggaggtag cagccttttg cagtgctctt 2460 tacaggtata acaggttcac tcagcgccac tcacttatag gtggcctgtg gctgcatccc 2520 gaggggttgt tgggtatttt cccccctttc tctcctgggc acctctggga gtctgctaac 2580 cctttttgtg gggagggaac cttgtacacc cggacatggt caacatctgg tttttctagt 2640 gacttctccc cccctgaggc agccgccgca gcgccggctg ctgccccggg gctacgccac 2700 cctaaacctc ctgttagtga catttgggtg ttaccgccat cttctgaaga attccaggtt 2760 ggcacagcgc ccgctccccc tgcccctgcg cccgctcaac catccaaccc cgccgggcca 2820 aaggcccccg tgcgtaagct gccaatgctt cgagggttaa cgaatgttgc gcaggtatgt 2880 gttgatgttg tatcccgagt ttgcggagtt agccctggac cgccatcttc tgaagaattt 2940 caggttggca cagcgcccgc tccccctgcc cctgcgcccg ctcaaccatc caaccccgcc 3000 gggccaaagg cccccgtgcg taagctgcca atgccaccat ccccgcgcac ccgccgcctt 3060 ctttacacct atccggatgg ggcaaaggtg tatgtagggt cactgtttga gtctgactgt 3120 gattggctgg ttaatgcgtc gaatcccggc catcgccctg gaggcggcct ttgccatgcc 3180 ttctaccaac gctaccccga gtctttccac ccaactgagt tcattatgcg cgacggtctt 3240 gccgcgtata ctttaactcc ccggcctatt attcatgcag tggctcctga ctatagggtt 3300 gagcataacc ccaagaggct tgaggcggca taccgagaga cttgctctcg ccgcggtacc 3360 gccgcctacc cactcctcgg ctcgggtata taccaagttc ccgtcagcct cagctttgac 3420 gcttgggagc gtaaccaccg ccccggggat gagctctacc taaccgacct cgccgctacc 3480 tggttcgagg ctaataagcc aacgcagccg accctcacaa taactgagga tgcggcccgt 3540 acagccaacc tagcactgga gatcgatgct gctacggagg tcggccgggc ttgtgccggc 3600 tgcgcagtta gtcctggggt tgtgcactat cagtttactg ctggggtccc aggttcgggg 3660 aagtcgcgct ctatacagca gggggatgtt gatgtggtgg ttgttcccac tcgggagctc 3720 cggaatagtt ggcgtcgccg gggtttcgcg gcttttacac ctcatacggc ggcccgtgtc 3780 accacgggcc gtcgtgttgt gattgatgag gccccatccc tcccaccgca tttgttgcta 3840 ctacacatgc agcgggcctc gtcggtccac cttctcggcg acccaaacca gatccctgcc 3900 atagacttcg agcatgccgg cctggtcccc gcaatacgtc ctgagcttgc gcccaccagt 3960 tggtggcatg tcacccatcg ctgccccgct gacgtgtgcg agcttatacg cggggcctat 4020 cccaaaatcc aaaccactag ccgcgtgctg cggtctttat tctggaatga gcctgccatc 4080 ggccagaagt tagtttttac tcaggctgct aaggctgcca accccggtgc gattacagtc 4140 cacgaggccc agggcgctac tttcacggag actacaatca tagccacggc tgatgctagg 4200 gggctcatcc aatcttcccg agcccatgcc atagtcgcac tcacccgcca cacagagaag 4260 tgcgttattc ttgatgcccc cggcttgtta cgtgaggtcg gtatatcgga tgtgattgtc 4320 aacaattttt tcctcgccgg cggggaggtg ggtcaccatc gcccctctgt gataccccgc 4380 ggctatcctg accagaacct cgcgacacta caggcctttc caccttcctg ccagattagt 4440 gcctaccacc agctagctga ggaacttggc caccgcccag ctcccgtcgc cgctgtcttg 4500 cccccttgcc ctgaacttga gcagggcttg ttatatatgc ctcaggagct tacggtgtct 4560 gatagcgtgc tagtctttga actcacggac atagtccact gccggatggc cgcccccagc 4620 cagcggaagg ccgtcctatc gacgctcgtg ggtaggtatg gccgtcggac gaagctgtat 4680 gaagcagctc actctgacgt ccgtgagtcc ctggctagat tcatccccac cattgggcct 4740 gttcaggcta ccacgtgtga gttatatgag ctggttgagg ccatggtgga gaaaggtcag 4800 gatggctctg ccgtgcttga gctcgacctt tgcaatcgtg atgtatcgcg tatcacattt 4860 ttccagaaag attgtaacaa attcaccaca ggggagacca ttgcccacgg taaggttggc 4920 cagggcatct cggcttggag taagaccttt tgtgccctgt ttggtccgtg gtttcgtgct 4980 attgaaaaag aaatactagc cctgctccca cctaatattt tctacggcga cgcatacgag 5040 gagtctgtgt ttgccgccgc tgtgtcgggg gcaggttcaa gcatggtatt tgagaatgat 5100 ttctcagagt ttgatagcac ccaaaataac ttctcccttg gtctcgagtg tgtagtcatg 5160 gaggaatgtg gcatgcccca gtggctaatc cggttgtacc atttggtccg gtcggcctgg 5220 atcctacagg cgccgaagga gtctctcaag ggattttgga agaagcattc tggtgagccc 5280 ggcaccctcc tctggaacac tgtttggaat atggcgatca tagcacattg ctatgaattt 5340 cgtgatttta gagttgccgc tttcaaggga gatgattccg tggtcctctg tagcgactac 5400 cgtcagagcc gcaatgcagc ggccctgatt gcaggttgcg ggctcaaact gaaggttgat 5460 tatcgcccca ttgggttgta tgctggtgtg gtggtggccc ctggcctagg gacgctaccc 5520 gatgtggtgc gctttgccgg ccggctgtct gagaagaact ggggccctgg gccggagcgg 5580 gctgagcagt tgcgcctagc tgtttgtgac tttcttcgag ggttaacgaa tgttgcgcag 5640 gtatgtgttg atgttgtatc ccgagtttac ggggttagcc ctgggctggt acataacctt 5700 attggcatgt tgcaaaccat agctgatggc aaagcccatt ttacagagac tgtcaaacct 5760 gtgcttgacc tcacgaactc tatcatacag cgggtggaat gaataacatg ttttgtgcat 5820 tgcccatggg atcaccatgc gccctagggc tgttctgttg ctgttcttca tgcttctgcc 5880 tatgctgccc gcgccaccgg ccggccagcc gtctggccgc cgtcgtgggc ggcgcagcgg 5940 cggtaccggc agtggtttct ggggtgacag ggttgattct cagcccttcg ccctccccta 6000 tattcatcca accaacccct ttgccgccga tgtcgtaccg caatccgggg ctagagctcg 6060 ccctcgacag ccaccccgcc ccctcggctc ctcttggcgc gatcagtccc agcgcccctc 6120 tgctgtccca cgtcgtcgat ctgccccagc cggggctgcg ccgctgactg ccgtttcacc 6180 tgctcccgac acagctcctg tacctgatgt tgactcgcgc ggcgccatac tgcgacgcca 6240 gtacaattta tccacatccc cgctcacatc atctgttgct tcgggcacta atctggttct 6300 ttatgccgcc ccgctaaacc ctttgctgcc ccttcaggat ggcactaaca ctcatatcat 6360 ggccactgag gcatctaatt atgcccagta tcgggttgtc cgagctacga tccgttacag 6420 gccattggtg ccaaatgctg tcggcggcta tgcaatatcc atctcattct ggcctcagac 6480 tactactacc cccacgtctg ttgatatgaa ctctattact tccactgatg ttaggattct 6540 agttcaaccc ggcattgcct ctgagttggt cattcctagt gagcgcctcc attatcgtaa 6600 ccagggctgg cgctctgtgg agacctctgg cgtggctgaa gaggaggcta cttctggttt 6660 ggtaatgctt tgtatccatg gctctcctgt taattcctac accaataccc cctataccgg 6720 ggcgcttgga ctccttgatt tcgctttaga gcttgagttt aggaatttga cacccgggaa 6780 caccaacact cgtgtgtccc ggtatacaag cacagcccgt catcggctgc gccgcggtgc 6840 tgatggcacc gccgaactta ccaccacagc ggccacgcgc tttatgaagg acctgcactt 6900 caccggtacg aatggggtcg gtgaggtggg tcgtggtatt gctctcacac tctttaatct 6960 tgctgacacg cttctcggtg gtttgccgac agaattaatt tcgtcggccg ggggacagtt 7020 attctactcc cgccccgtcg tctcagccaa tggcgagccg actgttaagt tatatacatc 7080 tgtagagaat gcgcagcagg ataaagggat cgctatccca catgacatag atctgggtga 7140 ctcccgtgtg gtcatccaag actatgacaa ccagcatgag caggatcgac ccaccccctc 7200 gcctgcccct tctcgccctt tttcggttct tcgcgctaat gatgtcctat ggctttctct 7260 tactgccgct gagtacgacc agactacata tgggtcgtcc actaacccga tgtatgtctc 7320 ggatactgtc acatttgtca atgtggctac aggcgcccag gctgtcgccc gttccctcga 7380 ctggtctaaa gttactctgg acggccgtcc tcttactact atccagcagt actccaaaac 7440 attttatgtt ctcccgctcc gcgggaagtt atctttttgg gaggccggga caactaaggc 7500 cggttacccc tataattata acacaactgc tagtgatcag attctgatcg aaaatgcggc 7560 cggtcatcgt gttgctattt ccacgtatac caccagcttg ggcgctggcc ctgtgtctgt 7620 ttctgcagtc ggtgttttag ccccacattc ggctcttgca gtccttgaag acactattga 7680 ctactctgcc cgtgcccaca cttttgatga tttctgcccg gagtgtcgcg ctcttggttt 7740 gcaggggtgt gccttccagt ctactattgc tgagcttcag cgtcttaaaa tgaaggtagg 7800 taaaacccgg gagttttaat caatttcctt tgtgccccct tcatagcttt tgctttattt 7860 cttttttctg cgtttcgcgc tcccaggaaa aaaaaaaaaa aaaaaaaaaa tttaaatatc 7920 agttctggac cagcgagctg tgctgcgact cgtggcgtaa tcatggtcat agctgtttcc 7980 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 8040 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 8100 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 8160 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 8220 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 8280 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 8340 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 8400 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 8460 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 8520 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 8580 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 8640 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 8700 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 8760 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg 8820 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 8880 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 8940 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 9000 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 9060 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 9120 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 9180 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 9240 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 9300 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 9360 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 9420 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 9480 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 9540 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 9600 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 9660 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 9720 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 9780 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 9840 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 9900 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 9960 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 10020 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 10080 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 10140 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcg 10194 // ID MT051993; SV 1; linear; other DNA; STD; SYN; 834 BP. XX AC MT051993; XX DT 30-JUN-2020 (Rel. 144, Created) DT 30-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct CHIS1754T gene, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RC Publication Status: Available-Online prior to print RP 1-834 RA Han Y., Guan F., Sun J., Wu N., Tian J.; RT "Identification of a chitosanase from the marine metagenome and its RT molecular improvement based on evolution data"; RL Appl Microbiol Biotechnol:(2020). XX RN [2] RP 1-834 RA Guan F., Tian J., Wu N., Han Y.; RT ; RL Submitted (03-FEB-2020) to the INSDC. RL Institute of Biotechnology, Chinese Academy of Agricultural Sciences, 12 RL Zhongguancun South Street, Beijing, Beijing 100081, China XX DR MD5; 0dfea96124fb92a64a9721e20b08ccc5. XX FH Key Location/Qualifiers FH FT source 1..834 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..>834 FT /codon_start=1 FT /transl_table=11 FT /product="CHIS1754T" FT /EC_number="3.2.1.132" FT /note="multi-point mutant of chis1754" FT /protein_id="QKX95860.1" FT /translation="MTKKPGTRLAAVVLGAALAAGLPLGTASAAPETVHSATQRAGADL FT DEPHKKEIAMELVSSAENSSLDWKAQYRYIEDIGDGRGYTAGIIGFTSGTGDMLELVEY FT YTKAKPDNVLAKYLPALRKVNGSDSHDGLDPDFTADWHKAAKDPAFQKAQNDERDRVYF FT DPAVDQAKKDGLRALGQFIYYDAMVMHGPGDDDTSFGGIRHRALEKAKPPAQGGDEREY FT LDAFLDARKAAMKTEEAHSDTSRVDTEQRVFLDKGNFDLNPPLHWKVYGDSYTIPE" XX SQ Sequence 834 BP; 191 A; 216 C; 282 G; 145 T; 0 other; atgaccaaga aaccgggtac ccgtctggcg gcggtggttc tgggtgcggc gctggcggcg 60 ggtctgccgc tgggtaccgc gagcgcggcg ccggaaaccg ttcacagcgc gacccagcgt 120 gcgggtgcgg atctggacga accgcacaag aaagagatcg cgatggaact ggtgagcagc 180 gcggagaaca gcagcctgga ctggaaggcg caataccgtt atatcgaaga tattggtgac 240 ggccgtggtt acaccgcggg catcattggt tttaccagcg gcaccggtga tatgctggag 300 ctggttgagt actataccaa ggcgaaaccg gacaacgtgc tggcgaagta tctgccggcg 360 ctgcgtaaag tgaacggcag cgatagccac gacggtctgg acccggactt caccgcggat 420 tggcacaagg cggcgaaaga cccggcgttt cagaaagcgc aaaacgatga gcgtgaccgt 480 gtttacttcg atccggcggt tgaccaggcg aagaaagatg gcctgcgtgc gctgggtcaa 540 tttatctact atgacgcgat ggttatgcac ggcccgggtg acgatgacac cagcttcggt 600 ggcattcgtc accgtgcgct ggaaaaagcg aaaccgccgg cgcagggtgg cgatgagcgt 660 gaatatctgg atgcgttcct ggacgcgcgt aaggcggcga tgaaaaccga ggaagcgcac 720 agcgatacca gccgtgtgga caccgagcaa cgtgttttcc tggataaggg caactttgac 780 ctgaacccgc cgctgcactg gaaagtgtac ggtgacagct ataccattcc ggaa 834 // ID MT237562; SV 1; linear; other DNA; STD; SYN; 1488 BP. XX AC MT237562; XX DT 30-JUN-2020 (Rel. 144, Created) DT 30-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct DNA photolyase class II gene, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RC Publication Status: Available-Online prior to print RP 1-1488 RA Marizcurrena J.J., Acosta S., Canclini L., Hernandez P., Valles D., RA Lamparter T., Castro-Sowinski S.; RT "A natural occurring bifunctional CPD/(6-4)-photolyase from the Antarctic RT bacterium Sphingomonas sp. UV9"; RL Appl Microbiol Biotechnol:(2020). XX RN [2] RP 1-1488 RA Marizcurrena J.J., Castro Sowinski S.; RT ; RL Submitted (23-MAR-2020) to the INSDC. RL Bioquimica, Facultad de Ciencias, Igua, Montevideo, Montevideo 11400, RL Uruguay XX DR MD5; bac5a8ef0875c2471ce9efe2358aa1e2. XX CC ##Assembly-Data-START## CC Assembly Method :: SPAdes v. 3.14.0 CC Coverage :: 40 CC Sequencing Technology :: Illumina CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1488 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /country="Antarctica" FT /note="derived from Sphingomonas sp. UV9; codon-optimized FT for expression in E. coli" FT /db_xref="taxon:32630" FT CDS 1..1488 FT /codon_start=1 FT /transl_table=11 FT /product="DNA photolyase class II" FT /EC_number="4.1.99.3" FT /note="PhrA; DNA repair enzyme" FT /protein_id="QKY77621.1" FT /translation="MPAILPHSLSSWSEAPRVRALNARDVRPDGRYVLCWLQQALRARD FT NPVIDAAIHLGNALGLPVIVYHGLREDYPYASDRLHRFILGASRDLARECRQRGLACVQ FT YVDRADAREKGLVYRLAADSAAVLVEDQPTFVARWQSERFAARIERAVFAVNAACLVPP FT AVLGDGIGSTTAFRRRHQPVRSDWQETTDAAPAVAPYDGPLPYTPDRLEACSDTDLDHL FT VATAAIDHTLPVSTMFPAGREGALVHLERLMDTVLPTYAATRNDATNPVGASMLSPYLH FT FGVIGPRDVMATVEAADAPAASKEKFADELLTWREWFHYQARTLPAPERYDRIAEWAQE FT TLEAHAVDPRPDIETLAALLHGETRDETWNACQRQFLIDGWMHNNLRMYWGKRIIAMTP FT DPQTAWATACYLNDRLSLDGRDPSTYGNIAATFGGGSPGREGPSVYGKVPTRSDGSTRH FT RPGGPAWLHAAATRSRPDPAVPSDLPTDLYLAGKSPL" XX SQ Sequence 1488 BP; 243 A; 435 C; 521 G; 289 T; 0 other; atgccggcga tcctgccgca cagcctgagc agctggagcg aggcgccgcg tgtgcgtgcg 60 ctgaacgcgc gtgatgtgcg tccggacggt cgttacgttc tgtgctggct gcagcaagcg 120 ctgcgtgcgc gtgataaccc ggttatcgac gcggcgattc atctgggtaa cgcgctgggt 180 ctgccggtga tcgtttatca cggcctgcgt gaggattacc cgtatgcgag cgaccgtctg 240 caccgtttta ttctgggtgc gagccgtgac ctggcgcgtg aatgccgtca gcgtggcctg 300 gcgtgcgtgc aatacgttga tcgtgcggac gcgcgtgaga agggtctggt gtatcgtctg 360 gcggcggata gcgcggcggt gctggttgaa gaccagccga ccttcgttgc gcgttggcaa 420 agcgagcgtt ttgcggcgcg tatcgaacgt gcggtgttcg cggttaacgc ggcgtgcctg 480 gtgccgccgg cggttctggg cgatggtatt ggcagcacca ccgcgttccg tcgtcgtcac 540 cagccggtgc gtagcgattg gcaagaaacc accgatgcgg cgccggcggt tgcgccgtac 600 gatggtccgc tgccgtatac cccggaccgt ctggaagcgt gcagcgacac cgatctggac 660 cacctggttg cgaccgcggc gattgaccac accctgccgg ttagcaccat gtttccggcg 720 ggtcgtgagg gtgcgctggt gcacctggaa cgtctgatgg ataccgttct gccgacctac 780 gcggcgaccc gtaacgatgc gaccaacccg gtgggtgcga gcatgctgag cccgtatctg 840 cacttcggtg ttattggccc gcgtgatgtg atggcgaccg ttgaggcggc ggatgcgccg 900 gcggcgagca aggagaaatt tgcggatgaa ctgctgacct ggcgtgaatg gttccactat 960 caagcgcgta ccctgccggc gccggagcgt tatgaccgta tcgcggaatg ggcgcaagaa 1020 accctggaag cgcatgcggt ggatccgcgt ccggacattg aaaccctggc ggcgctgctg 1080 catggcgaga cccgtgatga aacctggaac gcgtgccagc gtcaatttct gatcgacggt 1140 tggatgcaca acaacctgcg tatgtactgg ggcaaacgta tcattgcgat gaccccggat 1200 ccgcagaccg cgtgggcgac cgcgtgctac ctgaacgacc gtctgagcct ggatggtcgt 1260 gacccgagca cctatggcaa cattgcggcg acctttggtg gcggtagccc gggtcgtgaa 1320 ggtccgagcg tgtacggcaa ggttccgacc cgtagcgatg gcagcacccg tcaccgtccg 1380 ggcggtccgg cgtggctgca cgctgcggcg acccgtagcc gtccggaccc ggcggttccg 1440 agcgatctgc cgaccgacct gtatctggcg ggtaaaagcc cgctgtaa 1488 // ID MN544408; SV 1; linear; other DNA; STD; SYN; 1377 BP. XX AC MN544408; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct GDHB gene, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1377 RA Wagschal K.C., Chan V.J.; RT "Characterization of Acinetobacter calcoaceticus Glucose Dehydrogenase: RT Oxidation of Uronic Acids to Aldarates"; RL Unpublished. XX RN [2] RP 1-1377 RA Wagschal K.C., Chan V.J.; RT ; RL Submitted (07-OCT-2019) to the INSDC. RL BRU, USDA, 800 Buchanan Street, Albany, CA 94702, USA XX DR MD5; fef73b366be6cfc0abd543dff1f6d0cb. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1377 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..>1377 FT /codon_start=1 FT /transl_table=11 FT /product="GDHB" FT /EC_number="1.1.5.2" FT /note="Acinetobacter sGDHB; no signal sequence; N-terminal FT Met, C-terminal GlyGlySerGly" FT /protein_id="QKV50545.1" FT /translation="MDVPLTPSQFAKAKSENFDKKVILSNLNKPHALLWGPDNQIWLTE FT RATGKILRVNPESGSVKTVFQVPEIVNDADGQNGLLGFAFHPDFKNNPYIYISGTFKNP FT KSTDKELPNQTIIRRYTYNKSTDTLEKPVDLLAGLPSSKDHQSGRLVIGPDQKIYYTIG FT DQGRNQLAYLFLPNQAQHTPTQQELNGKDYHTYMGKVLRLNLDGSIPKDNPSFNGVVSH FT IYTLGHRNPQGLAFTPNGKLLQSEQGPNSDDEINLIVKGGNYGWPNVAGYKDDSGYAYA FT NYSAAANKSIKDLAQNGVKVAAGVPVTKESEWTGKNFVPPLKTLYTVQDTYNYNDPTCG FT EMTYICWPTVAPSSAYVYKGGKKAITGWENTLLVPSLKRGVIFRIKLDPTYSTTYDDAV FT PMFKSNNRYRDVIASPDGNVLYVLTDTAGNVQKDDGSVTNTLENPGSLIKFTYKAKGGS FT G" XX SQ Sequence 1377 BP; 366 A; 379 C; 388 G; 244 T; 0 other; atggatgtgc cgctgacccc gagccagttc gcgaaggcga aaagcgaaaa ctttgacaag 60 aaagttatcc tgagcaacct gaacaaaccg cacgcgctgc tgtggggtcc ggataaccaa 120 atctggctga ccgagcgtgc gaccggtaaa attctgcgtg ttaacccgga aagcggcagc 180 gtgaagaccg ttttccaggt gccggagatt gtgaacgacg cggatggtca aaacggcctg 240 ctgggtttcg cgtttcaccc ggacttcaaa aacaacccgt acatctatat tagcggtacc 300 tttaagaacc cgaaaagcac cgataaggaa ctgccgaacc agaccatcat tcgtcgttac 360 acctataaca agagcaccga caccctggag aaaccggttg atctgctggc gggcctgccg 420 agcagcaaag accaccagag cggccgtctg gtgatcggtc cggaccaaaa gatctactat 480 accattggcg atcagggtcg taaccaactg gcgtacctgt ttctgccgaa ccaggcgcaa 540 cacaccccga cccagcaaga gctgaacggt aaagattacc acacctatat gggcaaggtg 600 ctgcgtctga acctggacgg tagcatcccg aaagataacc cgagcttcaa cggcgtggtt 660 agccacattt acaccctggg ccaccgtaac ccgcaaggtc tggcgtttac cccgaacggc 720 aagctgctgc agagcgagca aggcccgaac agcgacgatg aaatcaacct gattgttaaa 780 ggtggcaact acggctggcc gaacgtggcg ggttataagg acgatagcgg ctacgcgtat 840 gcgaactaca gcgcggcggc gaacaagagc atcaaagacc tggcgcagaa cggtgtgaaa 900 gttgcggcgg gcgtgccggt taccaaggag agcgaatgga ccggtaaaaa cttcgttccg 960 ccgctgaaga ccctgtacac cgtgcaagac acctacaact ataacgatcc gacctgcggc 1020 gaaatgacct atatctgctg gccgaccgtt gcgccgagca gcgcgtacgt gtataagggt 1080 ggcaagaaag cgattaccgg ttgggagaac accctgctgg ttccgagcct gaaacgtggc 1140 gtgatcttcc gtattaagct ggacccgacc tacagcacca cctatgacga tgcggttccg 1200 atgtttaaaa gcaacaaccg ttaccgtgac gtgattgcga gcccggatgg taacgttctg 1260 tatgtgctga ccgataccgc gggtaacgtt cagaaggacg atggcagcgt gaccaacacc 1320 ctggagaatc cgggcagcct gatcaagttc acctacaaag cgaagggcgg cagcggt 1377 // ID MN550906; SV 1; linear; other DNA; STD; SYN; 1173 BP. XX AC MN550906; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct reverse adipate degradation pathway protein 0875 gene, DE partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1173 RA Zhao Y.; RT ; RL Submitted (08-OCT-2019) to the INSDC. RL National Engineering Laboratory for Cereal Fermentation Technology (NELCF), RL Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China., Wuxi, RL Jiangsu 214122, China XX DR MD5; 9e07caf78aafabe27c3c3a418c2eaa8c. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1173 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Thermobifida fusca" FT /db_xref="taxon:32630" FT CDS 1..>1173 FT /codon_start=1 FT /transl_table=11 FT /product="reverse adipate degradation pathway protein 0875" FT /note="Tfu_0875_sce; RAPD enzyme; codon optimized for S. FT cerevisiae" FT /protein_id="QKV50739.1" FT /translation="MTDVYILDAVRTPFGRYGGALSGIRPDDLAAHVLRALAERSPGLD FT PAAVDDVFFGDANGAGEDNRNVARMAALLAGWPTSVPGVTLNRLCGSGMESVIAANRAI FT AVGDASLAVAGGVESMSRAPWVLPKPAQGFPTGHETLYSTTLGWRMVNPAMPEQWTVSL FT GESTEQVAATYGISRAEQDAFALRSHERAARAWAEGVFDAEITQIPGAELERDESIRET FT SAEKLAALKPAFRPDGTITAGNASPLNDGAAALLIGDAAAAERVGREPLARIVSRGVAA FT VDPDVFGIGPVQAAEIALRRAGIGWDDLSVVELNEAFAAQSLACLKLWPDLDPEIVNPN FT GGAIAIGHPLGASGARIVGTLAHELHRRGGGWGLAAICIGVGQGLAVVLHR" XX SQ Sequence 1173 BP; 279 A; 274 C; 380 G; 240 T; 0 other; atgactgacg tttatatctt ggacgcagta agaaccccat tcggaagata tgggggagcg 60 cttagcggca tcagaccgga tgatctagcc gcccatgtac tacgtgcgtt agctgagagg 120 agcccggggt tagatcctgc ggccgtcgac gatgttttct tcggtgacgc aaacggtgca 180 ggcgaagaca accgtaatgt tgcccgtatg gcggctttgc tggcggggtg gcctacaagt 240 gtgcccgggg taactcttaa ccgtttatgc ggatcaggta tggagagcgt cattgcagca 300 aatagagcca tcgctgttgg tgacgcgtca cttgctgtcg caggcggtgt cgagtccatg 360 tccagggcac cgtgggtctt gccgaaacca gcacaagggt ttcccaccgg gcacgagact 420 ctatacagca cgactttagg gtggcgtatg gtaaatcccg ctatgccgga gcagtggacc 480 gtttcactag gcgaaagtac agaacaggtg gcagcaacat acggcatatc cagggcggag 540 caagatgctt tcgcactgag gagtcatgag cgtgctgcgc gtgcgtgggc agaaggggtc 600 tttgacgctg agattacaca aattcctgga gcagaactgg aaagagatga aagcattaga 660 gaaacctctg cggaaaaact agccgcctta aagccagcgt ttaggccgga cggaacaatt 720 acggcaggaa acgcatcacc actaaacgac ggagccgcgg ccttgctaat cggagacgcg 780 gcggcagcag aaagggttgg cagggagcct ttggccagga ttgtcagcag aggtgtagcg 840 gctgtggacc cggatgtctt tggtattggt ccagtacagg cagcagaaat tgcattaaga 900 cgtgccggga ttggctggga tgacttatca gtcgtcgagc ttaacgaagc attcgcggct 960 cagtccttag cctgtctaaa gttgtggccg gaccttgacc ctgaaatagt taacccaaac 1020 ggaggcgcga ttgccatagg gcatccctta ggggcatcag gtgcaaggat tgttgggact 1080 ctagcgcacg aactacacag gaggggcgga gggtggggac tagcggcaat atgtattggc 1140 gtagggcagg gcttggcagt cgtactgcac aga 1173 // ID MN550907; SV 1; linear; other DNA; STD; SYN; 1194 BP. XX AC MN550907; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct reverse adipate degradation pathway protein 2399 gene, DE partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1194 RA Zhao Y.; RT ; RL Submitted (08-OCT-2019) to the INSDC. RL National Engineering Laboratory for Cereal Fermentation Technology (NELCF), RL Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China., Wuxi, RL Jiangsu 214122, China XX DR MD5; cb490020a22f782e003d6a0e1e75725e. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1194 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Thermobifida fusca" FT /db_xref="taxon:32630" FT CDS 1..>1194 FT /codon_start=1 FT /transl_table=11 FT /product="reverse adipate degradation pathway protein 2399" FT /note="Tfu_2399_sce; RAPD enzyme; codon optimized for S. FT cerevisiae" FT /protein_id="QKV50740.1" FT /translation="MVEEINKVGVVGLGTMGAGIVEVFARAGFTVTGVEIDDAALERGR FT THLEKSLAKAVAKGKLTEDEQRAILGRVTFTTSRDDLADAHLAVEAVPERLDIKRSVFA FT DLDRILPPAAILATNTSSLSVTEIAALTSRPGKVIGLHFFNPAPVMRLVEIVTTVVTEP FT HVRETATQVVTRLGKTPVAVGDRAGFVANALLVPYLNHAVAVYEQGLATREQIDAAITS FT AAGFPMGPLTLMDLVGLDVLLDVMDVLWDEFRRPRYAAAPLLRRMVAAGLLGRKSGRGF FT YDYSGADNPAEPEPTAPLAQLVGDGPGQISLADLLLVPHLNDAARMIGDGYATADDVDT FT AMRLGCGYPKGLAAMLDERGVKNVTETLAELAAAGLFTDDTAPLLTMLAKQGKDTLRS" XX SQ Sequence 1194 BP; 293 A; 264 C; 362 G; 275 T; 0 other; atggttgaag aaataaataa agttggtgtc gtaggactag ggacaatggg ggctggtata 60 gtagaggttt ttgcgagggc ggggtttacg gttacaggag tggaaattga cgacgcagct 120 cttgaaaggg gacgtaccca tttggagaaa agtctggcca aagccgtggc gaagggaaag 180 ctaactgagg atgaacaaag agccatattg ggtagggtaa ctttcaccac aagcagggat 240 gaccttgctg atgctcatct tgcggtcgaa gctgtcccag aaaggctaga tattaaaagg 300 tccgtcttcg ccgacttgga tagaatcctg cctccagctg ctatactggc tacgaacacg 360 agttcattat ctgtgacgga aattgccgct ttaacatcca gacccggtaa agtcattggt 420 ttgcactttt ttaatccagc tccggttatg aggctagttg aaatcgtcac gacagtggtg 480 acggaacccc acgtgagaga gactgccaca caggtagtga cgcgtttggg taaaacgccg 540 gtggcagttg gtgaccgtgc tggttttgtc gctaacgcct tgttggtgcc gtatctaaat 600 cacgcggtcg cggtttacga gcaaggcctt gctacgaggg agcaaataga cgcagctatt 660 acgtccgctg caggtttccc aatggggccg ttgaccctaa tggatctggt gggattggac 720 gtgctgctag atgttatgga tgtactgtgg gacgagttta ggagaccacg ttatgccgct 780 gccccactac tgaggaggat ggtagccgct ggcctattag gcagaaaaag cggcagaggc 840 ttctatgact atagcggcgc cgataaccct gctgagcccg agccgacggc accccttgcc 900 caacttgttg gggatggacc aggccaaatc tctttagcgg atctattatt agttccgcac 960 ctgaatgacg cagctaggat gataggcgat gggtatgcga ccgccgacga tgtggataca 1020 gccatgcgtc tgggttgcgg ttaccccaag ggcttagccg ccatgctgga cgagcgtgga 1080 gtcaagaacg tgactgagac tttggcagag ctggcagcgg caggactttt tacggatgat 1140 acagcaccgt tgcttacaat gttagcgaag caaggaaaag acactctgag aagc 1194 // ID MN550908; SV 1; linear; other DNA; STD; SYN; 768 BP. XX AC MN550908; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct reverse adipate degradation pathway protein 0067 gene, DE partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-768 RA Zhao Y.; RT ; RL Submitted (08-OCT-2019) to the INSDC. RL National Engineering Laboratory for Cereal Fermentation Technology (NELCF), RL Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China., Wuxi, RL Jiangsu 214122, China XX DR MD5; eb61f3be8d555afc4953cb4066436adf. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..768 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Thermobifida fusca" FT /db_xref="taxon:32630" FT CDS 1..>768 FT /codon_start=1 FT /transl_table=11 FT /product="reverse adipate degradation pathway protein 0067" FT /note="Tfu_0067_sce; RAPD enzyme; codon optimized for S. FT cerevisiae" FT /protein_id="QKV50741.1" FT /translation="MGEFIRFESDGPVRHIVLNAPQRLNALDRPMLAELAEAVRAVAAD FT EEARALVVSGAGRAFCAGADVTSLFGDPTRPPAVIRDELKQVYASFLSIADLTIPTIAA FT VGGIAVGAGVNIAMACDMVVAGPKAKFAITFAEMGLHPGGGCSWFLTRRMGGHRALATL FT LDAERIDAEEAFRAGLVTRLVEDPVAEALAMAHSYAERDPGLVRDMKRAVRMAETADLA FT TVLEFESWAQASSVNSPRFQEFLAEFAARKNKKE" XX SQ Sequence 768 BP; 186 A; 168 C; 233 G; 181 T; 0 other; atgggtgagt tcattagatt tgagtctgac gggcccgtga gacatatagt gttaaatgca 60 ccccaaagac ttaatgcact tgatcgtcct atgttagctg aattagctga ggccgttcgt 120 gccgtggccg cggatgaaga ggcccgtgca ttggttgtga gcggcgctgg tagagccttc 180 tgcgctgggg ccgatgtcac ctcattgttt ggcgatccaa cccgtccccc ggcagtaatc 240 cgtgatgaac taaaacaagt atatgcgtct tttctgtcta tcgctgatct gacgattccc 300 accattgccg ccgtaggggg tattgctgtg ggagcgggag taaacatagc gatggcctgt 360 gacatggtcg ttgctgggcc aaaagccaaa ttcgctatca cttttgccga aatggggtta 420 catcctgggg gtgggtgttc ctggttcctt acaaggagga tggggggtca cagggcactg 480 gcgaccctac ttgatgctga gaggattgat gcggaagaag cattcagggc cggattagtt 540 acaaggctag tggaagatcc cgtggctgaa gcgctggcaa tggcacacag ctatgctgag 600 agagatcctg gccttgttcg tgatatgaaa agggcagtca gaatggcaga aaccgccgac 660 ctagctactg tgctagaatt tgaaagctgg gcacaagcaa gtagcgtcaa tagcccaaga 720 tttcaggagt tcctggcaga gtttgcagcg aggaaaaaca aaaaagaa 768 // ID MN550909; SV 1; linear; other DNA; STD; SYN; 1155 BP. XX AC MN550909; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct reverse adipate degradation pathway protein 1647 gene, DE partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1155 RA Zhao Y.; RT ; RL Submitted (08-OCT-2019) to the INSDC. RL National Engineering Laboratory for Cereal Fermentation Technology (NELCF), RL Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China., Wuxi, RL Jiangsu 214122, China XX DR MD5; 49daf953e4f01b2d0554205b95d5bc66. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1155 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Thermobifida fusca" FT /db_xref="taxon:32630" FT CDS 1..>1155 FT /codon_start=1 FT /transl_table=11 FT /product="reverse adipate degradation pathway protein 1647" FT /note="Tfu_1647_sce; RAPD enzyme; codon optimized for S. FT cerevisiae" FT /protein_id="QKV50742.1" FT /translation="MSDFDLYRPTEEHEALREAIRSVAEDKIAPHAADVDEQSRFPQEA FT YEALRASDFHAPHVAEEYGGVGADALATCIVIEEIARVCASSSLIPAVNKLGSMPLILS FT GSDEVKQRYLPELASGEAMFSYGLSEREAGSDTASMRTRAVRDGDDWILNGQKSWITNA FT GISKYYTVMAVTDPDGPRGRNISAFVVHIDDPGFSFGEPERKLGIKGSPTRELIFDNVR FT IPGDRLVGKVGEGLRTALRTLDHTRVTIGAQAVGIAQGALDYALGYVKERKQFGKAIAD FT FQGIQFMLADMAMKLEAARQMVYVAAAKSERDDADLSFYGAAAKCFASDVAMEITTDAV FT QLLGGYGYTRDYPVERMMRDAKITQIYEGTNQIQRVVMARQLLKK" XX SQ Sequence 1155 BP; 313 A; 229 C; 338 G; 275 T; 0 other; atgtctgatt tcgatttata tagaccgacc gaagagcatg aagcattaag ggaggccatt 60 aggtccgtgg cagaggataa aatagctcct cacgctgcag atgtggatga gcagagcagg 120 ttcccacaag aagcgtacga ggctctgcgt gcaagtgact tccacgctcc tcatgtggct 180 gaagagtacg gcggtgtcgg cgctgatgcg ctggcgactt gtattgtaat tgaagaaatc 240 gccagagtat gtgcgagctc ctccttaatc ccagcagtga acaaattggg tagtatgcct 300 ttgatactgt ctgggagtga cgaagtaaaa cagcgttact tgcccgaatt ggccagcgga 360 gaggctatgt ttagttatgg gctatctgaa agggaagctg gtagtgacac tgcctccatg 420 agaactcgtg cggtgagaga cggggatgac tggatactga atggccaaaa gtcctggata 480 accaatgctg gaatttccaa gtactatacc gttatggctg taactgaccc agacggaccc 540 aggggtagga atattagcgc atttgtagta cacatagatg atcccggttt tagtttcgga 600 gaacctgaga gaaagctagg aataaagggg tctccaactc gtgagctaat cttcgataat 660 gttaggattc cgggggatag attggtgggt aaggtcggtg aaggtttaag gactgctcta 720 aggactttgg accatacgag ggtaactatt ggagctcaag ccgttgggat tgcgcagggc 780 gcattagatt acgcacttgg ttatgtaaag gagaggaagc agttcggtaa ggcaattgct 840 gacttccagg ggatccaatt catgctggct gatatggcca tgaaattaga ggctgcacgt 900 cagatggtgt atgtcgccgc agcgaaatct gaacgtgatg acgccgactt atccttttac 960 ggcgcggcag caaagtgctt tgcgtccgat gtcgcaatgg aaataaccac cgacgccgtt 1020 cagttgttag gcgggtatgg atatactaga gactatccag tagaacgtat gatgcgtgat 1080 gccaaaataa cgcagatcta cgaaggtacg aatcagatcc aacgtgtagt catggcaagg 1140 cagctgctga aaaaa 1155 // ID MN550910; SV 1; linear; other DNA; STD; SYN; 876 BP. XX AC MN550910; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct reverse adipate degradation pathway protein 2576 gene, DE partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-876 RA Zhao Y.; RT ; RL Submitted (08-OCT-2019) to the INSDC. RL National Engineering Laboratory for Cereal Fermentation Technology (NELCF), RL Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China., Wuxi, RL Jiangsu 214122, China XX DR MD5; 5e54245090e3fe0742de09e341c5550f. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..876 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Thermobifida fusca" FT /db_xref="taxon:32630" FT CDS 1..>876 FT /codon_start=1 FT /transl_table=11 FT /product="reverse adipate degradation pathway protein 2576" FT /note="Tfu_2576_sce; RAPD enzyme; codon optimized for S. FT cerevisiae" FT /protein_id="QKV50743.1" FT /translation="MAIFLTKDSKVLVQGMTGSEGTKHTRRMLAAGTNIVGGVNPRKAG FT QVVDFDGTQVPVFGSVAEGMKATGADVTVIFVPPKFAKDAVIEAIDAEIGLAVVITEGI FT PVHDTATFWAHACSKGNKTRIIGPNCPGLITPGQSNAGIIPADITKPGRIGLVSKSGTL FT TYQMMYELRDIGFSTCVGIGGDPIIGTTHIDALAAFEADPDTDVIVMIGEIGGDAEERA FT AEYIKKHVTKPVVGYIAGFTAPEGKTMGHAGAIVSGSSGTAAAKKEALEAVGVKVGKTP FT SEAAKLVRSLF" XX SQ Sequence 876 BP; 227 A; 180 C; 260 G; 209 T; 0 other; atggctatct tcttaaccaa agactccaaa gtccttgtgc agggcatgac tgggagtgag 60 ggaacaaagc ataccagaag aatgttggca gccgggacaa acatagttgg cggggttaac 120 ccgcgtaagg cgggtcaagt tgtggacttc gatggtacac aggtgcctgt attcggatca 180 gtcgctgagg gtatgaaagc tactggtgcc gatgtcaccg ttatctttgt accgccgaaa 240 tttgcaaaag atgctgtaat agaggcgatt gatgcggaga ttggtctagc tgtagttatc 300 actgagggca ttccggttca tgataccgca accttctggg ctcatgcttg cagtaagggt 360 aacaaaacac gtattattgg gcccaactgt cctgggctta ttactccagg ccaatcaaat 420 gcaggaataa tacccgccga tataacgaaa ccgggccgta ttggtcttgt aagtaagtcc 480 ggtacgctaa cttatcagat gatgtacgag ctaagggata tcgggtttag cacttgtgta 540 gggattggcg gggacccgat tatagggaca acccacattg acgcgctagc ggcttttgag 600 gcagaccccg ataccgatgt tatcgttatg atcggcgaaa ttggcgggga tgccgaggaa 660 agggccgccg aatatataaa aaagcatgtt accaagccgg tagtcggtta catagctggt 720 ttcacagcac ctgagggtaa gactatgggg catgctggcg cgattgtatc tggtagctct 780 ggaacagccg ctgcgaagaa ggaggcgtta gaagccgtcg gagtaaaagt tggtaagact 840 ccgtctgagg ctgcgaaatt ggtgaggagt ttgttc 876 // ID MN550911; SV 1; linear; other DNA; STD; SYN; 1230 BP. XX AC MN550911; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct reverse adipate degradation pathway protein 2577 gene, DE partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1230 RA Zhao Y.; RT ; RL Submitted (08-OCT-2019) to the INSDC. RL National Engineering Laboratory for Cereal Fermentation Technology (NELCF), RL Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu 214122, China., Wuxi, RL Jiangsu 214122, China XX DR MD5; 8ed09253667fa530288c5cfe2bd1dcca. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1230 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Thermobifida fusca" FT /db_xref="taxon:32630" FT CDS 1..>1230 FT /codon_start=1 FT /transl_table=11 FT /product="reverse adipate degradation pathway protein 2577" FT /note="Tfu_2577_sce; RAPD enzyme; codon optimized for S. FT cerevisiae" FT /protein_id="QKV50744.1" FT /translation="MNDLRRNPASESQGRTLVDLFEYQAKALFAEYGVPVPQGKVASTP FT EEVRAIAEEFAAAGKPRVVVKAQVKTGGRGKAGGVKVADGPDDAVAKAKQILGMDIKGH FT TVHRVLVEEASDIAEEYYFSFLLDRANRSFLSICSAEGGMEIEEVAATNPDAVAKVPIS FT PLKGAPADVAADIVAQGKLPEAAAQGAVDVITKLWKVFVEKDATLVEVNPLILTKDGRV FT VALDGKVTLDDNAEFRQDLESLASAAEGDPLEVKAKEKGLNYVKLDGEVGIIGNGAGLV FT MSTLDVVAYAGEQHGGVKPANFLDIGGGASAEVMANGLEIILSDPAVKSVFVNVFGGIT FT ACDAVANGIVQALELLESRGEDVTKPLVVRLDGNNAELGRSILNERNHPAVRQVDTMDG FT AAALAAELAAK" XX SQ Sequence 1230 BP; 323 A; 236 C; 392 G; 279 T; 0 other; atgaacgact tgaggagaaa ccccgcttct gagtcccaag gcagaactct ggtggatttg 60 ttcgagtatc aggctaaggc gctatttgcg gaatatggcg tgcctgtccc acaagggaag 120 gtggcctcaa cgccagaaga agtgcgtgct atcgcagagg agtttgcggc agcggggaag 180 ccaagggtcg ttgtgaaggc gcaggttaag actggcggtc gtggaaaagc aggcggcgtg 240 aaggtggcgg atggtcccga tgacgccgtc gctaaagcaa aacaaatatt aggtatggat 300 ataaaaggcc atactgttca tcgtgtactt gtagaggaag cgagcgatat agctgaagaa 360 tattacttta gttttctgtt agacagggcg aacagaagtt tcctttcaat ttgctcagcg 420 gaaggcggaa tggagattga ggaagtcgct gccacaaacc cggacgcagt cgcgaaggtt 480 ccgatctcac cccttaaagg tgcccctgcc gatgtggcgg cagacatcgt agctcagggt 540 aaattgcctg aagctgccgc acagggggct gttgatgtta ttacgaaatt atggaaagta 600 tttgttgaga aggacgcgac ccttgtggaa gtgaatccac taattctgac aaaggatggt 660 cgtgttgtgg ctttagacgg caaggtcaca ctagacgata atgcggagtt taggcaggat 720 ttggagtctc ttgcatctgc tgccgaaggt gatcctctag aagtaaaagc taaggagaaa 780 ggtctgaatt atgtaaagtt ggacggagaa gtgggtataa tcgggaatgg tgctggctta 840 gtaatgagta cattagacgt cgtggcctac gcgggagagc aacatggagg agtaaaacct 900 gcgaactttc tggatatcgg tggcggtgca tctgcggaag ttatggctaa tgggttggag 960 ataattctgt ccgatcctgc agtgaagagt gtgtttgtta atgtctttgg cggcattaca 1020 gcgtgcgacg cagtggcgaa tggcattgtt caagcactag agctgcttga gagtaggggg 1080 gaggatgtga caaaacccct ggttgtaaga ttagacggga acaacgcgga actaggcagg 1140 tcaatactga acgagaggaa ccatcctgcc gtacgtcaag ttgacacgat ggacggggca 1200 gctgctctgg ccgcggaact agcggcgaaa 1230 // ID MT039000; SV 1; linear; other DNA; STD; SYN; 2019 BP. XX AC MT039000; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct lipoxygenase gene, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-2019 RA Hashem C., Stolterfoht H., Rinnofner C., Steinberger S., Winkler M., RA Pichler H.; RT "Secretion of Pseudomonas aeruginosa lipoxygenase by Pichia pastoris upon RT glycerol feed"; RL Unpublished. XX RN [2] RP 1-2019 RA Hashem C., Stolterfoht H., Rinnofner C., Steinberger S., Winkler M., RA Pichler H.; RT ; RL Submitted (07-FEB-2020) to the INSDC. RL TU Graz, Institute of molecular biotechnology, Petersgasse 14, Graz, Styria RL 8010, Austria XX DR MD5; 283e2054d74f0a00db67f4abc1a248f2. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..2019 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Pseudomonas aeruginosa PAO1 and FT optimized for Komagataella phaffii PaLOX" FT /db_xref="taxon:32630" FT CDS <1..2019 FT /codon_start=1 FT /transl_table=11 FT /product="lipoxygenase" FT /EC_number="1.13.11.33" FT /protein_id="QKV49803.1" FT /translation="HHHHHHNDSIFFSPLKYLGAEQQRSIDASRSLLDNLIPPSLPQYD FT NLAGKLARRAVLTSKKLVYVWTENFGNVKGVPMARSVPLGELPNVDWLLKTAGVIVELI FT VNFVASLPASAAAQFERIATGLSGDLEAARQVHEALLEEAKNDPAAAGSLLLRFTELQT FT RVIAILTRVGLLVDDILKSASNLVTQRGQGDGLNRFRAVFGTLRLPEVADSFRDDEAFA FT YWRVAGPNPLLIRRVDALPANFPLGEEQFRRVMGADDSLLEAAASRRLYLLDYAELGKL FT APSGAVDKLLTGTGFAYAPIALFALGKDRARLLPVAIQCGQDPATHPMFVRPAESESDL FT YWGWQMAKTVVQVAEENYHEMFVHLAQTHLVSEAFCLATQRTLAPSHPLHVLLAPHFEG FT TLFINEGAARILLPSAGFIDVMFAAPIQDTQATAGGNRLGFDFYRGMLPESLKARNVDD FT PLALPDYPYRDDGLLVWNAIRQWAADYVAVYYASDGDVTADVELAAWVGEVIGSGKVAG FT FRPITGRSQLVEVLTMVIFTASAQHAAVNFPQPSMMTYAPAICAMSAAPAPDSPSGKSE FT ADWLKMMPPTLVALEKVNIYHLLGSVYHGRLGDYRQTGFPYAPVFSDRRVTASGGPLER FT FQARLKEVEATIRTRNQARRRPYEYLLPSRIPASTNI" XX SQ Sequence 2019 BP; 500 A; 451 C; 491 G; 577 T; 0 other; caccatcatc accatcataa cgattctatt ttcttttctc cacttaagta cttgggtgcc 60 gaacaacagc gttctattga tgctagtaga tccttgttgg ataatctgat cccaccttca 120 cttccacaat atgacaactt agctggtaag cttgcaagaa gggctgtact gacatcaaag 180 aagttggtgt atgtctggac cgagaacttt ggtaatgtga agggagttcc aatggctagg 240 tctgttccac taggtgaatt acctaacgtt gattggcttt tgaagactgc tggagttatt 300 gtagagttga ttgttaactt tgtcgcttca ctgccagcct cagcagctgc tcagtttgag 360 agaatcgcta caggtctatc tggtgactta gaagctgcaa gacaagtgca cgaagctctt 420 ttggaagagg ctaagaatga ccctgctgca gctggctctc tgttacttag attcaccgaa 480 ctgcagacca gagtgattgc tatccttact agagtaggtc tgttggttga cgacatactg 540 aaatcagcat ccaacttggt tacacagaga ggtcaaggag atggacttaa tcgtttcaga 600 gccgttttcg gtacactgag attgcctgag gtagccgatt cctttagaga cgatgaggcc 660 tttgcctact ggagagtggc aggtccaaac ccattattga ttcgtagggt cgatgctcta 720 cccgcaaact tcccattggg agaggaacaa ttccgtaggg tgatgggagc tgacgatagt 780 ctgttagaag ccgctgcatc taggagactg tacctgttgg attacgccga attgggaaaa 840 cttgctccaa gtggagccgt agataaactg cttactggta ctggatttgc atacgcacca 900 atcgctttgt tcgccctggg taaagatagg gctaggctat taccagtcgc catacaatgt 960 ggacaagatc cagccaccca cccaatgttt gtgaggccag ctgaatccga aagtgatttg 1020 tattggggat ggcagatggc taagaccgtc gttcaagttg ccgaagagaa ctatcacgaa 1080 atgttcgtac atcttgccca aactcatctt gtatctgagg ctttctgcct ggcaactcag 1140 aggacacttg caccatccca ccctttgcat gtactgcttg caccacactt cgaaggaact 1200 ttgttcatca acgaaggtgc tgcaagaatc ctattaccat cagcaggttt cattgatgtg 1260 atgtttgctg ccccaattca agacactcag gcaacagctg gaggcaatcg tttgggtttt 1320 gacttttatc gtggtatgct tcctgaatct ttgaaagcaa gaaacgttga tgaccctctg 1380 gcactacctg actaccctta cagagatgac ggtcttctgg tttggaacgc aataagacaa 1440 tgggctgcag attacgttgc tgtgtactac gctagtgatg gcgacgttac agctgatgtt 1500 gagttggctg cctgggtcgg tgaggtgatt ggctctggaa aggttgctgg attcagacca 1560 atcaccggac gttcccaatt ggtggaggtt ttgacgatgg ttattttcac agcctccgct 1620 caacatgcag ctgttaattt tcctcaacca tctatgatga cttacgctcc tgctatttgt 1680 gctatgtctg cagctcctgc tcctgattcc ccttccggaa aatctgaagc tgattggttg 1740 aaaatgatgc ccccaactct agttgccttg gagaaagtca atatctatca tttgctgggt 1800 agtgtgtatc acggtaggct tggtgattat agacaaacgg gatttcccta cgctccagtt 1860 ttctctgaca gacgtgttac ggcctctgga ggtccattgg agagatttca agctagattg 1920 aaggaggttg aagctactat aagaacccgt aaccaagcta gaagacgtcc ttatgagtat 1980 ttgctgccat ctagaatacc tgcttctact aatatttga 2019 // ID MT328400; SV 1; circular; other DNA; STD; SYN; 5754 BP. XX AC MT328400; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning vector pMESD2, complete sequence. XX KW . XX OS Cloning vector pMESD2 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-5754 RA Steyaert J., Uchanski T., Pardon E.; RT "Megabodies expand the nanobody toolkit for protein structure determination RT by single-particle cryo-EM"; RL Unpublished. XX RN [2] RP 1-5754 RA Steyaert J., Uchanski T., Pardon E.; RT ; RL Submitted (10-APR-2020) to the INSDC. RL VIB-VUB Center for Structural Biology, VIB, Pleinlaan 2, Brussels 1050, RL Belgium XX DR MD5; 5ccce72857bd2a7e07b51b8c7b477fdd. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..5754 FT /organism="Cloning vector pMESD2" FT /mol_type="other DNA" FT /db_xref="taxon:2741117" FT regulatory 143..198 FT /note="pLac" FT /regulatory_class="promoter" FT misc_feature 225..281 FT /note="DsbA signal peptide" FT misc_feature 282..320 FT /note="nanobody beta-strain A (residues 1-14)" FT misc_feature 321..1544 FT /note="cHopQ scaffold protein" FT misc_feature 1545..1547 FT /note="nanobody residue 17" FT misc_feature 1548..1580 FT /note="multi cloning site" FT misc_feature 1581..1598 FT /note="His6-tag" FT misc_feature 1599..1610 FT /note="EPEA-tag" FT misc_feature 1614..1643 FT /note="HA-tag" FT gene 1644..2877 FT /gene="M13 geneIII" FT rep_origin 3112..3567 FT /note="f1 ori" FT CDS 3954..4814 FT /codon_start=1 FT /transl_table=11 FT /product="ampR" FT /EC_number="3.5.2.6" FT /note="beta-bactamase; ampicillin resistance" FT /protein_id="QKV49908.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT rep_origin 4974..5588 FT /note="pMB1" XX SQ Sequence 5754 BP; 1506 A; 1378 C; 1366 G; 1504 T; 0 other; agagcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180 ttgtgagcgg ataacaattt cacaagctta aggagacagt acatatgaaa aagatttggc 240 tggcgctggc tggtttagtt ttagcgttta gcgcatcggc gcaggtgcag ctggtggagt 300 ctgggggagg actagtacag actaaaacaa caacttctgt tattgatact actaatgatg 360 cccaaaatct tttgactcaa gcgcaaacta ttgtcaatac ccttaaagat tattgcccta 420 tcttgatcgc aaaatctagt agtagcaatg gtggcacaaa caatgctaac accccttcat 480 ggcaaacagc cggtggcggc aaaaattcat gcgctacttt tggtgcagag tttagtgccg 540 cttcagacat gattaataat gcacaaaaaa tcgttcaaga aacccaacaa ctgagcgcca 600 accaaccaaa aaatatcaca caacctcata atctgaacct taacagccct agcagtctta 660 ccgctttagc tcaaaaaatg ctgaaaaacg cgcaatctca agcagaaatt ttaaaacttg 720 ccaatcaagt ggagagcgat tttaacaaac tttcttcagg ccatcttaaa gactacattg 780 ggaaatgtga cgcctctgct atcagtagtg ctaatatgac aatgcagaat cagaagaaca 840 actgggggaa cgggtgcgct ggcgtggaag aaactcagtc actgttaaaa acaagcgccg 900 ctgattttaa caaccaaact cctcaaatca atcaagctca aaacctggcc aacaccctta 960 ttcaagaact tggcaacaac ccttttcgtg ctagcggtgg aggatcagga ggtggaggct 1020 caggtaaact ttcagacact tatgaacagc taagccgtct tttaaccaac gataatggca 1080 caaactcaaa gacaagcgcc caagctatca accaagcggt taataatttg aacgaacgtg 1140 caaaaacttt agccggtggg acaaccaatt ctccggcata tcaagccacg cttttagcct 1200 tacgttctgt gttagggctg tggaatagca tgggttatgc tgtcatctgc ggaggttata 1260 ccaaaagtcc aggcgaaaac aatcaaaaag atttccacta caccgatgag aatggcaacg 1320 gcactacaat caattgcggt gggagcacaa atagtaatgg cactcatagt tataatggca 1380 caaatacatt aaaagcagac aaaaatgttt ctctttctat tgagcaatat gaaaaaatcc 1440 atgaagccta tcagattctt tcaaaagcct taaaacaagc cgggcttgct cctttaaata 1500 gcaaagggga aaagttagaa gcacatgtaa ccacatcaaa gtatggatcc ggaagagctt 1560 agtcgacaag ctcttcaagt caccaccatc accatcacga acctgaagcc tagtacccgt 1620 acgacgttcc ggactacggt tccactgttg aaagttgttt agcaaaacct catacagaaa 1680 attcatttac taacgtctgg aaagacgaca aaactttaga tcgttacgct aactatgagg 1740 gctgtctgtg gaatgctaca ggcgttgtcg tttgtactgg tgacgaaact cagtgttacg 1800 gtacatgggt tcctattggg cttgctatcc ctgaaaatga gggtggtggc tctgagggtg 1860 gcggttctga gggtggcggt tctgagggtg gcggtactaa acctcctgag tacggtgata 1920 cacctattcc gggctatact tatatcaacc ctctcgacgg cacttatccg cctggtactg 1980 agcaaaaccc cgctaatcct aatccttctc ttgaggagtc tcagcctctt aatactttca 2040 tgtttcagaa taataggttc cgaaataggc agggtgcatt aactgtttat acgggcactg 2100 ttactcaagg cactgacccc gttaaaactt attaccagta cactcctgta tcatcaaaag 2160 ccatgtatga cgcttactgg aacggtaaat tcagagactg cgctttccat tctggcttta 2220 atgaggatcc attcgtttgt gaatatcaag gccaatcgtc tgacctgcct caacctcctg 2280 tcaatgctgg cggcggctct ggtggtggtt ctggtggcgg ctctgagggt ggcggctctg 2340 agggtggcgg ctctgagggt ggcggttctg agggtggcgg ctctgagggt ggcggttccg 2400 gtggcggctc cggttccggt gattttgatt atgaaaaaat ggcaaacgct aataaggggg 2460 ctatgaccga aaatgccgat gaaaacgcgc tacagtctga cgctaaaggc aaacttgatt 2520 ctgtcgctac tgattacggt gctgctatcg atggtttcat tggtgacgtt tccggccttg 2580 ctaatggtaa tggtgctact ggtgattttg ctggctctaa ttcccaaatg gctcaagtcg 2640 gtgacggtga taattcacct ttaatgaata atttccgtca atatttacct tctttacctc 2700 agtcggttga atgtcgccct tatgtctttg gcgctggtaa accatatgaa ttttctattg 2760 attgtgacaa aataaactta ttccgtggtg tctttgcgtt tcttttatat gttgccacct 2820 ttatgtatgt attttcgacg tttgctaaca tactgcgtaa taaggagtct taataagaat 2880 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 2940 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 3000 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc 3060 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct 3120 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 3180 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 3240 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 3300 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct 3360 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 3420 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt 3480 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 3540 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg 3600 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 3660 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 3720 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat 3780 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg 3840 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 3900 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 3960 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 4020 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 4080 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 4140 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 4200 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 4260 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 4320 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 4380 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 4440 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 4500 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 4560 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 4620 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 4680 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 4740 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 4800 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 4860 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 4920 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 4980 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 5040 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 5100 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 5160 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 5220 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 5280 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 5340 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 5400 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 5460 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 5520 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 5580 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 5640 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 5700 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag 5754 // ID MT338520; SV 1; circular; other DNA; STD; SYN; 4434 BP. XX AC MT338520; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning vector pMESD22c7, complete sequence. XX KW . XX OS Cloning vector pMESD22c7 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-4434 RA Steyaert J., Uchanski T., Pardon E.; RT "Megabodies expand the nanobody toolkit for protein structure determination RT by single-particle cryo-EM"; RL Unpublished. XX RN [2] RP 1-4434 RA Steyaert J., Uchanski T., Pardon E.; RT ; RL Submitted (14-APR-2020) to the INSDC. RL VIB-VUB Center for Structural Biology, VIB, Pleinlaan 2, Brussels 1050, RL Belgium XX DR MD5; 4f92552d6c49a22247ebe53d7bd37fcb. XX FH Key Location/Qualifiers FH FT source 1..4434 FT /organism="Cloning vector pMESD22c7" FT /mol_type="other DNA" FT /db_xref="taxon:2741114" FT regulatory 143..198 FT /note="pLac" FT /regulatory_class="promoter" FT misc_feature 225..281 FT /note="DsbA signal peptide" FT misc_feature 282..320 FT /note="nanobody beta-strain A (residues 1-14)" FT misc_feature 321..1487 FT /note="c7HopQ scaffold protein" FT misc_feature 1488..1490 FT /note="nanobody residue 17" FT misc_feature 1491..1523 FT /note="multi cloning site" FT misc_feature 1524..1541 FT /note="His6-tag" FT misc_feature 1542..1553 FT /note="EPEA-tag" FT rep_origin 1792..2247 FT /note="f1 ori" FT CDS 2634..3494 FT /codon_start=1 FT /transl_table=11 FT /product="ampR" FT /EC_number="3.5.2.6" FT /note="beta-bactamase; ampicillin resistance" FT /protein_id="QKV49903.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT rep_origin 3654..4268 FT /note="pMB1" XX SQ Sequence 4434 BP; 1208 A; 1095 C; 1025 G; 1106 T; 0 other; agagcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180 ttgtgagcgg ataacaattt cacaagctta aggagacagt acatatgaaa aagatttggc 240 tggcgctggc tggtttagtt ttagcgttta gcgcatcggc gcaggtgcag ctggtggagt 300 ctgggggagg actagtacag actaaaacaa caacttctgt tattgatact actaatgatg 360 cccaaaatct tttgactcaa gcgcaaacta ttgtcaatac ccttaaagat tattgcccta 420 tcttgatcgc aaaatctagt agtagcaatg gtggcacaaa caatgctaac accccttcat 480 ggcaaacagc cggtggcggc aaaaattcat gcgctacttt tggtgcagag tttagtgccg 540 cttcagacat gattaataat gcacaaaaaa tcgttcaaga aacccaacaa ctgagcgcca 600 accaaccaaa aaatatcaca caacctcata atctgaacct taacagccct agcagtctta 660 ccgctttagc tcaaaaaatg ctgaaaaacg cgcaatctca agcagaaatt ttaaaacttg 720 ccaatcaagt ggagagcgat tttaacaaac tttcttcagg ccatcttaaa gactacattg 780 ggaaatgtga cgcctctgct atcagtagtg ctaatatgac aatgcagaat cagaagaaca 840 actgggggaa cgggtgcgct ggcgtggaag aaactcagtc actgttaaaa acaagcgccg 900 ctgattttaa caaccaaact cctcaaatca atcaagctca aaacctggcc aacaccctta 960 ttcaagaact tggcaacaac acttatgaac agctaagccg tcttttaacc aacgataatg 1020 gcacaaactc aaagacaagc gcccaagcta tcaaccaagc ggttaataat ttgaacgaac 1080 gtgcaaaaac tttagccggt gggacaacca attctccggc atatcaagcc acgcttttag 1140 ccttacgttc tgtgttaggg ctgtggaata gcatgggtta tgctgtcatc tgcggaggtt 1200 ataccaaaag tccaggcgaa aacaatcaaa aagatttcca ctacaccgat gagaatggca 1260 acggcactac aatcaattgc ggtgggagca caaatagtaa tggcactcat agttataatg 1320 gcacaaatac attaaaagca gacaaaaatg tttctctttc tattgagcaa tatgaaaaaa 1380 tccatgaagc ctatcagatt ctttcaaaag ccttaaaaca agccgggctt gctcctttaa 1440 atagcaaagg ggaaaagtta gaagcacatg taaccacatc aaagtatgga tccggaagag 1500 cttagtcgac aagctcttca agtcaccacc atcaccatca cgaacctgaa gcctaagaat 1560 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat 1620 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat 1680 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc 1740 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct 1800 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg 1860 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg 1920 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac 1980 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct 2040 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt 2100 tccaaactgg aacaacactc aaccctatct cgggctattc ttttgattta taagggattt 2160 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt 2220 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg 2280 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt 2340 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc 2400 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat 2460 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg 2520 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 2580 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 2640 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 2700 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 2760 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 2820 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 2880 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 2940 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 3000 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 3060 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 3120 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 3180 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 3240 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 3300 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 3360 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 3420 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 3480 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 3540 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 3600 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 3660 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 3720 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 3780 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 3840 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 3900 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 3960 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 4020 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 4080 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 4140 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 4200 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 4260 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 4320 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 4380 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag 4434 // ID MT338521; SV 1; circular; other DNA; STD; SYN; 5601 BP. XX AC MT338521; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning vector pMESP23E2, complete sequence. XX KW . XX OS Cloning vector pMESP23E2 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-5601 RA Steyaert J., Uchanski T., Pardon E.; RT "Megabodies expand the nanobody toolkit for protein structure determination RT by single-particle cryo-EM"; RL Unpublished. XX RN [2] RP 1-5601 RA Steyaert J., Uchanski T., Pardon E.; RT ; RL Submitted (14-APR-2020) to the INSDC. RL VIB-VUB Center for Structural Biology, VIB, Pleinlaan 2, Brussels 1050, RL Belgium XX DR MD5; 3d38e2b5bffadff35cb0c1877e6954a2. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..5601 FT /organism="Cloning vector pMESP23E2" FT /mol_type="other DNA" FT /db_xref="taxon:2741115" FT regulatory 143..198 FT /note="pLac" FT /regulatory_class="promoter" FT misc_feature 225..290 FT /note="pelB signal peptide" FT misc_feature 291..326 FT /note="nanobody beta-strain A (residues 1-13)" FT misc_feature 330..2654 FT /note="cYgjK scaffold protein" FT misc_feature 2658..2690 FT /note="multi cloning site" FT misc_feature 2691..2708 FT /note="His6-tag" FT misc_feature 2709..2720 FT /note="EPEA-tag" FT rep_origin 2959..3414 FT /note="f1 ori" FT CDS 3801..4661 FT /codon_start=1 FT /transl_table=11 FT /product="ampR" FT /EC_number="3.5.2.6" FT /note="beta-bactamase; ampicillin resistance" FT /protein_id="QKV49904.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT rep_origin 4821..5435 FT /note="pMB1" XX SQ Sequence 5601 BP; 1409 A; 1410 C; 1428 G; 1354 T; 0 other; agagcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180 ttgtgagcgg ataacaattt cacaagctta aggagacagt acatatgaaa tacctattgc 240 ctacggcagc cgctggattg ttattactcg cggcccagcc ggccatggcc caggtgcagc 300 tggtggagtc tgggggagga ctagtatata aagaagagac gcagtctggg ctgaacaact 360 acgcccgtgt ggtggagaaa ggccagtacg acagtctgga aattcctgca caggttgctg 420 catcgtggga atcggggcgt gatgatgccg ccgtctttgg gtttatcgac aaagaacagt 480 tggataaata tgtcgctaat ggcggcaaac gtagcgactg gacagtgaaa ttcgccgaaa 540 accgtagtca ggacggaaca ttgctgggct actcgctttt gcaggagtcg gtggatcagg 600 ccagctatat gtacagcgat aaccattatc tggcagagat ggcaaccatc ctgggtaagc 660 cagaagaggc caaacgttat cgtcagttgg cacagcagct ggcagactac atcaacacct 720 gtatgttcga ccctactacc cagttctact atgacgtacg tattgaagat aaaccactgg 780 ctaacggctg cgcaggcaaa cctattgttg agcgtggtaa agggccagaa ggctggtcgc 840 cactgtttaa cggtgcagca actcaggcca atgccgacgc tgtggtgaag gtgatgcttg 900 atcctaaaga gttcaacacc tttgtcccac tgggaactgc cgctttaacc aatcctgctt 960 ttggcgctga catctactgg cgtgggcgtg tatgggtgga tcagttctgg tttggtctga 1020 aagggatgga gcgttacggt tatcgtgatg atgccctgaa gctggcagat acattcttcc 1080 ggcacgccaa agggttaacc gccgatggac caattcagga aaattacaac ccactgacag 1140 gcgcacagca aggcgcacca aatttctcct ggagcgctgc tcatttgtat atgttgtata 1200 acgatttttt ccgtaagcaa gctagcggtg gaggatcagg aggtggagga tcaggaggtg 1260 gaggctcagg taacgccgac aattataaaa acgtgattaa ccgtactggg gcccctcagt 1320 acatgaagga ttacgattac gacgatcacc agcgttttaa tccatttttc gatcttggag 1380 cctggcatgg tcatctgttg ccagacggcc ctaacactat gggcggcttt ccaggcgttg 1440 ctctgctgac agaagagtac atcaacttta tggcaagcaa tttcgaccgt ctgaccgtct 1500 ggcaggacgg caagaaagta gactttacac tggaggcata cagtattcct ggtgctctgg 1560 tgcaaaaact gacagcaaaa gatgtgcaag tcgaaatgac tctgcgtttc gccactcctc 1620 gtacctcact tctggaaacc aaaatcacca gcaataaacc actggatctg gtgtgggacg 1680 gcgaactgct ggaaaaactg gaagctaaag aagggaaacc tctttccgat aaaaccattg 1740 ctggcgaata ccctgactat cagcgtaaaa tcagcgccac ccgtgatggc ctgaaagtca 1800 cctttggcaa agtgcgtgcc acctgggatc tgctgacctc cggcgaatcg gaatatcagg 1860 tgcataaatc cctgcctgtg cagactgaaa tcaacggtaa tcgttttacc agtaaggccc 1920 atatcaacgg ttcgaccaca ctttacacca cctattccca tctgctgacc gcacaggaag 1980 ttagcaaaga gcaaatgcag atccgtgata ttctggctcg tccagcattt tatctgaccg 2040 cctcgcagca acgttgggaa gaatacctga agaaagggtt aaccaatcca gatgcaaccc 2100 ctgaacagac tcgtgtcgct gtgaaagcaa tcgaaactct gaacggtaac tggcgttcac 2160 ctggcggtgc tgtgaaattt aacaccgtta caccatcggt gacagggcgt tggttctccg 2220 gcaatcagac ctggccttgg gatacctgga agcaggcttt tgctatggca catttcaatc 2280 cagacatcgc caaagagaat atccgtgctg tcttctcctg gcagatccag cctggcgata 2340 gcgtgcgtcc acaggatgtg ggctttgtcc cagacctgat tgcttggaac ctgagtccag 2400 agcgtggtgg cgatggcggc aactggaacg aacgtaatac caaacctagc cttgccgcct 2460 ggtcggtgat ggaagtgtat aacgtcaccc aagataaaac ctgggtggca gagatgtacc 2520 ctaaactggt ggcctatcac gactggtggt tacgtaaccg tgatcacaac ggcaacggcg 2580 tgccagaata tggcgcaacc cgtgacaaag cccacaacac tgagagcggc gagatgctgt 2640 ttacagtgaa aaaggattcc ggaagagctt agtcgacaag ctcttcaagt caccaccatc 2700 accatcacga acctgaagcc taagaattca ctggccgtcg ttttacaacg tcgtgactgg 2760 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg 2820 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc 2880 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 2940 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 3000 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 3060 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 3120 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg 3180 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 3240 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 3300 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 3360 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt 3420 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa 3480 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg 3540 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga 3600 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 3660 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt 3720 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat 3780 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt 3840 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg 3900 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga 3960 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc 4020 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac 4080 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg 4140 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca 4200 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg 4260 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg 4320 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg 4380 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag 4440 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg 4500 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct 4560 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac 4620 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact 4680 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga 4740 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt 4800 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct 4860 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc 4920 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc 4980 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc 5040 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg 5100 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt 5160 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg 5220 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg 5280 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt 5340 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag 5400 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt 5460 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta 5520 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt 5580 cagtgagcga ggaagcggaa g 5601 // ID MT338522; SV 1; circular; other DNA; STD; SYN; 5595 BP. XX AC MT338522; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning vector pMESP23NO, complete sequence. XX KW . XX OS Cloning vector pMESP23NO OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-5595 RA Steyaert J., Uchanski T., Pardon E.; RT "Megabodies expand the nanobody toolkit for protein structure determination RT by single-particle cryo-EM"; RL Unpublished. XX RN [2] RP 1-5595 RA Steyaert J., Uchanski T., Pardon E.; RT ; RL Submitted (14-APR-2020) to the INSDC. RL VIB-VUB Center for Structural Biology, VIB, Pleinlaan 2, Brussels 1050, RL Belgium XX DR MD5; bb5205cacf18c5b592779626963e0ebc. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..5595 FT /organism="Cloning vector pMESP23NO" FT /mol_type="other DNA" FT /db_xref="taxon:2741116" FT regulatory 143..198 FT /note="pLac" FT /regulatory_class="promoter" FT misc_feature 225..290 FT /note="pelB signal peptide" FT misc_feature 291..326 FT /note="nanobody beta-strain A (residues 1-13)" FT misc_feature 327..2651 FT /note="cYgjK scaffold protein" FT misc_feature 2652..2684 FT /note="multi cloning site" FT misc_feature 2685..2702 FT /note="His6-tag" FT misc_feature 2703..2714 FT /note="EPEA-tag" FT rep_origin 2953..3408 FT /note="f1 ori" FT CDS 3795..4655 FT /codon_start=1 FT /transl_table=11 FT /product="ampR" FT /EC_number="3.5.2.6" FT /note="beta-bactamase; ampicillin resistance" FT /protein_id="QKV49905.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT rep_origin 4815..5429 FT /note="pMB1" XX SQ Sequence 5595 BP; 1407 A; 1410 C; 1427 G; 1351 T; 0 other; agagcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 60 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 120 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 180 ttgtgagcgg ataacaattt cacaagctta aggagacagt acatatgaaa tacctattgc 240 ctacggcagc cgctggattg ttattactcg cggcccagcc ggccatggcc caggtgcagc 300 tggtggagtc tgggggagga ctagtaaaag aagagacgca gtctgggctg aacaactacg 360 cccgtgtggt ggagaaaggc cagtacgaca gtctggaaat tcctgcacag gttgctgcat 420 cgtgggaatc ggggcgtgat gatgccgccg tctttgggtt tatcgacaaa gaacagttgg 480 ataaatatgt cgctaatggc ggcaaacgta gcgactggac agtgaaattc gccgaaaacc 540 gtagtcagga cggaacattg ctgggctact cgcttttgca ggagtcggtg gatcaggcca 600 gctatatgta cagcgataac cattatctgg cagagatggc aaccatcctg ggtaagccag 660 aagaggccaa acgttatcgt cagttggcac agcagctggc agactacatc aacacctgta 720 tgttcgaccc tactacccag ttctactatg acgtacgtat tgaagataaa ccactggcta 780 acggctgcgc aggcaaacct attgttgagc gtggtaaagg gccagaaggc tggtcgccac 840 tgtttaacgg tgcagcaact caggccaatg ccgacgctgt ggtgaaggtg atgcttgatc 900 ctaaagagtt caacaccttt gtcccactgg gaactgccgc tttaaccaat cctgcttttg 960 gcgctgacat ctactggcgt gggcgtgtat gggtggatca gttctggttt ggtctgaaag 1020 ggatggagcg ttacggttat cgtgatgatg ccctgaagct ggcagataca ttcttccggc 1080 acgccaaagg gttaaccgcc gatggaccaa ttcaggaaaa ttacaaccca ctgacaggcg 1140 cacagcaagg cgcaccaaat ttctcctgga gcgctgctca tttgtatatg ttgtataacg 1200 attttttccg taagcaagct agcggtggag gatcaggagg tggaggatca ggaggtggag 1260 gctcaggtaa cgccgacaat tataaaaacg tgattaaccg tactggggcc cctcagtaca 1320 tgaaggatta cgattacgac gatcaccagc gttttaatcc atttttcgat cttggagcct 1380 ggcatggtca tctgttgcca gacggcccta acactatggg cggctttcca ggcgttgctc 1440 tgctgacaga agagtacatc aactttatgg caagcaattt cgaccgtctg accgtctggc 1500 aggacggcaa gaaagtagac tttacactgg aggcatacag tattcctggt gctctggtgc 1560 aaaaactgac agcaaaagat gtgcaagtcg aaatgactct gcgtttcgcc actcctcgta 1620 cctcacttct ggaaaccaaa atcaccagca ataaaccact ggatctggtg tgggacggcg 1680 aactgctgga aaaactggaa gctaaagaag ggaaacctct ttccgataaa accattgctg 1740 gcgaataccc tgactatcag cgtaaaatca gcgccacccg tgatggcctg aaagtcacct 1800 ttggcaaagt gcgtgccacc tgggatctgc tgacctccgg cgaatcggaa tatcaggtgc 1860 ataaatccct gcctgtgcag actgaaatca acggtaatcg ttttaccagt aaggcccata 1920 tcaacggttc gaccacactt tacaccacct attcccatct gctgaccgca caggaagtta 1980 gcaaagagca aatgcagatc cgtgatattc tggctcgtcc agcattttat ctgaccgcct 2040 cgcagcaacg ttgggaagaa tacctgaaga aagggttaac caatccagat gcaacccctg 2100 aacagactcg tgtcgctgtg aaagcaatcg aaactctgaa cggtaactgg cgttcacctg 2160 gcggtgctgt gaaatttaac accgttacac catcggtgac agggcgttgg ttctccggca 2220 atcagacctg gccttgggat acctggaagc aggcttttgc tatggcacat ttcaatccag 2280 acatcgccaa agagaatatc cgtgctgtct tctcctggca gatccagcct ggcgatagcg 2340 tgcgtccaca ggatgtgggc tttgtcccag acctgattgc ttggaacctg agtccagagc 2400 gtggtggcga tggcggcaac tggaacgaac gtaataccaa acctagcctt gccgcctggt 2460 cggtgatgga agtgtataac gtcacccaag ataaaacctg ggtggcagag atgtacccta 2520 aactggtggc ctatcacgac tggtggttac gtaaccgtga tcacaacggc aacggcgtgc 2580 cagaatatgg cgcaacccgt gacaaagccc acaacactga gagcggcgag atgctgttta 2640 cagtgaaaaa gtccggaaga gcttagtcga caagctcttc aagtcaccac catcaccatc 2700 acgaacctga agcctaagaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac 2760 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat 2820 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg 2880 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa 2940 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 3000 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt 3060 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 3120 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt 3180 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 3240 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt 3300 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt 3360 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc 3420 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg 3480 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg 3540 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa 3600 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 3660 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 3720 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 3780 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 3840 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 3900 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 3960 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 4020 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 4080 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 4140 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 4200 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 4260 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 4320 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 4380 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 4440 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 4500 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 4560 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 4620 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 4680 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 4740 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 4800 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 4860 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 4920 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 4980 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 5040 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 5100 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 5160 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 5220 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 5280 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 5340 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 5400 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 5460 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 5520 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 5580 gcgaggaagc ggaag 5595 // ID MT338523; SV 1; circular; other DNA; STD; SYN; 8011 BP. XX AC MT338523; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Yeast display vector pNMB2, complete sequence. XX KW . XX OS Yeast display vector pNMB2 OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-8011 RA Steyaert J., Uchanski T., Pardon E.; RT "Megabodies expand the nanobody toolkit for protein structure determination RT by single-particle cryo-EM"; RL Unpublished. XX RN [2] RP 1-8011 RA Steyaert J., Uchanski T., Pardon E.; RT ; RL Submitted (14-APR-2020) to the INSDC. RL VIB-VUB Center for Structural Biology, VIB, Pleinlaan 2, Brussels 1050, RL Belgium XX DR MD5; ee9640660c5c2506fc7ab09d0d11e3c3. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..8011 FT /organism="Yeast display vector pNMB2" FT /mol_type="other DNA" FT /db_xref="taxon:2741118" FT CDS 715..1575 FT /codon_start=1 FT /transl_table=11 FT /product="ampR" FT /EC_number="3.5.2.6" FT /note="beta-bactamase; ampicillin resistance" FT /protein_id="QKV49906.1" FT /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI FT ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS FT PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW FT EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA FT LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS FT LIKHW" FT regulatory 2940..3594 FT /note="GAL1/10" FT /regulatory_class="promoter" FT misc_feature 3632..3898 FT /note="appS4 leader sequence" FT misc_feature 3899..3937 FT /note="nanobody beta-strain A (residues 1-14)" FT misc_feature 3938..5161 FT /note="cHopQ scaffold protein" FT misc_feature 5162..5200 FT /note="multi cloning site" FT misc_feature 5201..5302 FT /note="linker" FT misc_feature 5303..5509 FT /note="Aga2p anchor protein" FT misc_feature 5531..5764 FT /note="ACP-tag" FT misc_feature 5768..5797 FT /note="Myc-tag" FT regulatory 5811..6117 FT /note="MFa1" FT /regulatory_class="terminator" FT rep_origin 6318..6745 FT /note="f1 ori" FT CDS complement(6870..7544) FT /codon_start=1 FT /transl_table=11 FT /product="Trp1" FT /note="phosphoribosylanthranilate isomerase; tryptophan FT biosynthesis" FT /protein_id="QKV49907.1" FT /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK FT RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES FT WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW FT VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA FT KK" XX SQ Sequence 8011 BP; 2338 A; 1839 C; 1660 G; 2174 T; 0 other; gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt 60 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata 120 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt 180 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa 240 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata 300 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct 360 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata 420 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact 480 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa 540 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg 600 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 660 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 720 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 780 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 840 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 900 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt 960 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1020 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1080 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1140 ccgaaggagc taaccgcttt ttttcacaac atgggggatc atgtaactcg ccttgatcgt 1200 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta 1260 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 1320 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 1380 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 1440 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 1500 ggcagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 1560 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 1620 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 1680 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1740 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 1800 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 1860 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 1920 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 1980 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2040 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2100 acgacctaca ccgaactgag atacctacag cgtgagcatt gagaaagcgc cacgcttccc 2160 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2220 agggagcttc caggggggaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2280 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc cgagcctatg gaaaaacgcc 2340 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2400 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 2460 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 2520 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac 2580 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttacctcact 2640 cattaggcac cccaggcttt acactttatg cttccggctc ctatgttgtg tggaattgtg 2700 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcggaatt 2760 aaccctcact aaagggaaca aaagctgggt acccgacagg ttatcagcaa caacacagtc 2820 atatccattc tcaattagct ctaccacagt gtgtgaacca atgtatccag caccacctgt 2880 aaccaaaaca attttagaag tactttcact ttgtaactga gctgtcattt atattgaatt 2940 ttcaaaaatt cttacttttt ttttggatgg acgcaaagaa gtttaataat catattacat 3000 ggcattacca ccatatacat atccatatct aatcttactt atatgttgtg gaaatgtaaa 3060 gagccccatt atcttagcct aaaaaaacct tctctttgga actttcagta atacgcttaa 3120 ctgctcattg ctatattgaa gtacggatta gaagccgccg agcgggtgac agccctccga 3180 aggaagactc tcctccgtgc gtcctcgtct tcaccggtcg cgttcctgaa acgcagatgt 3240 gcctcgcgcc gcactgctcc gaacaataaa gattctacaa tactagcttt tatggttatg 3300 aagaggaaaa attggcagta acctggcccc acaaaccttc aaatgaacga atcaaattaa 3360 caaccatagg atgataatgc gattagtttt ttagccttat ttctggggta attaatcagc 3420 gaagcgatga tttttgatct attaacagat atataaatgc aaaaactgca taaccacttt 3480 aactaatact ttcaacattt tcggtttgta ttacttctta ttcaaatgta ataaaagtat 3540 caacaaaaaa ttgttaatat acctctatac tttaacgtca aggagaaaaa accccggatc 3600 gaattcccta cttcatacat tttcaattaa gatgagattt ccttcaattt ttactgctgt 3660 tgtcttcgca gcatcctccg cattagctgc tccagcaaac actacagctg aagatgaaac 3720 ggcacaaatt ccagctgaag ctgtcatcgg ttacttaggt ttagaagggg atagcgatgt 3780 tgctgccttg ccattatccg atagcacaaa taacgggtca ttgtccacaa atactactat 3840 tgccagcatt gctgctaaag aagaaggggt acaattagat aaaagagagg ccgaagcaca 3900 ggtgcagctg gtggagtctg ggggaggact agtacagact aaaacaacaa cttctgttat 3960 tgatactact aatgatgccc aaaatctttt gactcaagcg caaactattg tcaataccct 4020 taaagattat tgccctatct tgatcgcaaa atctagtagt agcaatggtg gcacaaacaa 4080 tgctaacacc ccttcatggc aaacagccgg tggcggcaaa aattcatgcg ctacttttgg 4140 tgcagagttt agtgccgctt cagacatgat taataatgca caaaaaatcg ttcaagaaac 4200 ccaacaactg agcgccaacc aaccaaaaaa tatcacacaa cctcataatc tgaaccttaa 4260 cagccctagc agtcttaccg ctttagctca aaaaatgctg aaaaacgcgc aatctcaagc 4320 agaaatttta aaacttgcca atcaagtgga gagcgatttt aacaaacttt cttcaggcca 4380 tcttaaagac tacattggga aatgtgacgc ctctgctatc agtagtgcta atatgacaat 4440 gcagaatcag aagaacaact gggggaacgg gtgcgctggc gtggaagaaa ctcagtcact 4500 gttaaaaaca agcgccgctg attttaacaa ccaaactcct caaatcaatc aagctcaaaa 4560 cctggccaac acccttattc aagaacttgg caacaaccct tttcgtgcta gcggtggagg 4620 atcaggaggt ggaggctcag gtaaactttc agacacttat gaacagctaa gccgtctttt 4680 aaccaacgat aatggcacaa actcaaagac aagcgcccaa gctatcaacc aagcggttaa 4740 taatttgaac gaacgtgcaa aaactttagc cggtgggaca accaattctc cggcatatca 4800 agccacgctt ttagccttac gttctgtgtt agggctgtgg aatagcatgg gttatgctgt 4860 catctgcgga ggttatacca aaagtccagg cgaaaacaat caaaaagatt tccactacac 4920 cgatgagaat ggcaacggca ctacaatcaa ttgcggtggg agcacaaata gtaatggcac 4980 tcatagttat aatggcacaa atacattaaa agcagacaaa aatgtttctc tttctattga 5040 gcaatatgaa aaaatccatg aagcctatca gattctttca aaagccttaa aacaagccgg 5100 gcttgctcct ttaaatagca aaggggaaaa gttagaagca catgtaacca catcaaagta 5160 tggatccgga agagcttagt cgacaagctc ttcaagcttg ggaggtggct ctggtggagg 5220 gggatcagga ggtggtggct ccggtggagg tggttcgggc ggaggtggtt ccggtggagg 5280 aggttctggt ggaggcggta gccaggaact gacaactata tgcgagcaaa tcccctcacc 5340 aactttagaa tcgacaccat actctttgtc aacgactact attttagcca acgggaaggc 5400 aatgcaagga gtttttgaat attacaaatc agtaacgttt gtcagtaatt gcggttctca 5460 cccctcaaca actagcaaag gcagccccat aaacacacag tatgttttta aggacaatag 5520 ctcgacaagt atgagcacta tcgaagaacg tgttaagaaa attatcggcg aacagctggg 5580 cgttaagcag gaagaagtta ccaacaatgc ttctttcgtt gaagacctgg gcgccgattc 5640 tcttgacacc gttgagctgg taatggctct ggaagaagag tttgatactg agattccaga 5700 cgaagaagct gagaaaatca ccaccgttca ggctgccatt gattacatca acggccacca 5760 ggcaagcgaa caaaagttaa tttctgaaga ggacttgtaa tagcatgcag atctgataac 5820 aacagtgtag atgtaacaaa atcgactttg ttcccactgt acttttagct cgtacaaaat 5880 acaatatact tttcatttct ccgtaaacaa catgttttcc catgtaatat ccttttctat 5940 ttttcgttcc gttaccaact ttacacatac tttatatagc tattcacttc tatacactaa 6000 aaaactaaga caattttaat tttgctgcct gccatatttc aatttgttat aaattcctat 6060 aatttatcct attagtagct aaaaaaagat gaatgtgaat cgaatcctaa gagaattgag 6120 ctccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg 6180 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccccttcgc 6240 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 6300 gaatggcgaa tggcgcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 6360 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 6420 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 6480 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 6540 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 6600 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 6660 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 6720 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcctgatg 6780 cggtattttc tccttacgca tctgtgcggt atttcacacc gcaggcaagt gcacaaacaa 6840 tacttaaata aatactactc agtaataacc tatttcttag catttttgac gaaatttgct 6900 attttgttag agtcttttac accatttgtc tccacacctc cgcttacatc aacaccaata 6960 acgccattta atctaagcgc atcaccaaca ttttctggcg tcagtccacc agctaacata 7020 aaatgtaaac tttcggggct ctcttgcctt ccaacccagt cagaaatcga gttccaatcc 7080 aaaagttcac ctgtcccacc tgcttctgaa tcaaacaagg gaataaacga atgaggtttc 7140 tgtgaagctg cactgagtag tatgttgcag tcttttggaa atacgagtct tttaataact 7200 ggcaaaccga ggaactcttg gtattcttgc cacgactcat ctccatgcag ttggacgata 7260 tcaatgccgt aatcattgac cagagccaaa acatcctcct taggttgatt acgaaacacg 7320 ccaaccaagt atttcggagt gcctgaacta tttttatatg cttttacaag acttgaaatt 7380 ttccttgcaa taaccgggtc aattgttctc tttctattgg gcacacatat aatacccagc 7440 aagtcagcat cggaatctag agcacattct gcggcctctg tgctctgcaa gccgcaaact 7500 ttcaccaatg gaccagaact acctgtgaaa ttaataacag acatactcca agctgccttt 7560 gtgtgcttaa tcacgtatac tcacgtgctc aatagtcacc aatgccctcc ctcttggccc 7620 tctccttttc ttttttcgac cgaattaatt cttaatcggc aaaaaaagaa aagctccgga 7680 tcaagattgt acgtaaggtg acaagctatt tttcaataaa gaatatcttc cactactgcc 7740 atctggcgtc ataactgcaa agtacacata tattacgatg ctgtctatta aatgcttcct 7800 atattatata tatagtaatg tcgtttatgg tgcactctca gtacaatctg ctctgatgcc 7860 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 7920 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 7980 aggttttcac cgtcatcacc gaaacgcgcg a 8011 // ID MT450838; SV 1; linear; other DNA; STD; SYN; 1836 BP. XX AC MT450838; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct laccase mRNA, complete cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1836 RA Tulek A., Karatas E., Cakar M.M., Aydin D., Yilmazcan O., Binay B.; RT "A New Laccase from Madurella mycetomatis expressed in Pichia pastoris: RT From discovery to yielding biotechnological performance in textile RT industry"; RL Unpublished. XX RN [2] RP 1-1836 RA Binay B.; RT ; RL Submitted (08-MAY-2020) to the INSDC. RL Bioengineering, Gebze Technical University, Gebze, Kocaeli, Marmara Bolgesi RL 41400, Turkey XX DR MD5; 4b4e40b45aa6f74f7ae06b5dcef0f4bf. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1836 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Madurella mycetomatis sequence" FT /db_xref="taxon:32630" FT CDS 1..1836 FT /codon_start=1 FT /transl_table=11 FT /product="laccase" FT /note="codon optimized for expression in Pichia pastoris" FT /protein_id="QKV50993.1" FT /translation="MYWLLNRSAAACSAVLLTLLKSTLGSPAKPDPVVGLLPRQTIVPG FT GRPCGQHGPTNRGCWKNNWNISTDYEHDTPPAFNTHVFDFHITNVTDWLGPDGIRKPAM FT LINNQFPGPTLWVDWGDYIVINVYNDMQDNGTSIHWHGIRQFGESNQDGANGVTECPIP FT PGSSKTYDFHVTQYGTSWYHSHYSNQYGNGVVGALIARGPSSADYDIDLGPYMITDYYH FT ETADRLHLQAELVRNGPPPDSDNILFRGKNINPNGPGGSYDRLTLTPGKKHLIRLINAS FT VDNSFVVSLVGHNFTVVTTDLVPITPVVRSSLFLGVGQRYNVIVEANQAVDNYWFNATL FT ETNGNCGHSHNPYPAAIFQYEGASSTALPTNRGTPLVATCDGEKGFSPIVQRSVPPSQF FT RPSTLPITLEFPTHDRGQVFEWRVKNTPINVEWGHPVLEYVLENNDTFPGSLNLIEVSE FT PEVWTFWVIQNDFALPHPIHLHGHDFLVLGVGNGTFDPATMAGLLNFNNPIRRDVEQMP FT GAGWLVIAFKTDNPGCWLMHCHIGWHVAMGLGIQFLERKSDIRALMALDQMVPNCDAWR FT AYEPTSPYLPKIDSGLKRGVGVDMEGMEPAVRRIG" XX SQ Sequence 1836 BP; 442 A; 328 C; 403 G; 663 T; 0 other; atgtattggt tgttgaatag atccgctgct gcttgttctg ctgttttgtt gactttgttg 60 aagtctactt tgggttctcc agctaaacct gatccagttg ttggtttgtt gcctagacaa 120 actattgttc caggtggtag accttgtggt caacacggtc caactaatag aggttgttgg 180 aagaacaatt ggaacatttc tactgattac gaacatgata ctccacctgc tttcaatact 240 cacgtttttg atttccatat cactaacgtt actgattggt tgggtccaga tggtattaga 300 aagcctgcta tgttgattaa caaccaattt cctggtccaa ctttgtgggt tgattgggga 360 gattacatcg ttattaatgt ttacaacgat atgcaagata acggtacttc tattcattgg 420 cacggtatta gacaattcgg tgaatctaat caagatggtg ctaacggtgt tactgagtgt 480 ccaattccac ctggttcttc taagacttat gatttccatg ttactcaata cggtacttct 540 tggtatcatt ctcactactc taaccaatac ggtaacggtg ttgttggtgc tttgattgct 600 agaggtccat cttctgctga ttatgatatt gatttgggtc cttacatgat tactgattac 660 tatcacgaaa ctgctgatag attgcatttg caagctgagt tggttagaaa tggtccacct 720 ccagattctg ataacatctt gttcagaggt aaaaacatca accctaacgg tccaggtggt 780 tcttatgata gattgacttt gactccaggt aaaaagcact tgatcagatt gattaatgct 840 tctgttgata actctttcgt tgtttctttg gttggtcata acttcactgt tgttactact 900 gatttggttc ctattactcc agttgttaga tcctctttgt ttttgggtgt tggtcaaaga 960 tacaacgtta tcgttgaggc taaccaagct gttgataatt actggttcaa cgctactttg 1020 gaaactaatg gtaactgtgg tcattctcac aatccttatc cagctgctat ttttcaatac 1080 gagggtgctt cttctactgc tttgccaact aacagaggta ctcctttggt tgctacttgt 1140 gatggtgaaa agggtttttc tccaattgtt caaagatccg ttccaccatc tcaattcaga 1200 ccatctactt tgcctattac tttggaattt cctactcacg atagaggtca agttttcgag 1260 tggagagtta aaaatactcc aattaacgtt gaatggggtc atcctgtttt ggaatacgtt 1320 ttggagaaca atgatacttt cccaggttct ttgaatttga ttgaggtttc tgaacctgag 1380 gtttggactt tttgggttat tcaaaacgat ttcgctttgc ctcacccaat tcatttgcac 1440 ggtcatgatt tcttggtttt gggtgttggt aatggtactt tcgatccagc tactatggct 1500 ggtttgttga acttcaacaa cccaatcaga agagatgttg aacaaatgcc tggtgctggt 1560 tggttggtta ttgctttcaa gactgataat cctggttgtt ggttgatgca ctgtcatatt 1620 ggttggcatg ttgctatggg tttgggtatt caatttttgg agagaaagtc tgatattaga 1680 gctttgatgg ctttggatca aatggttcca aactgtgatg cttggagagc ttatgaacct 1740 acttctccat acttgcctaa gattgattct ggtttgaaaa gaggtgttgg tgttgatatg 1800 gagggtatgg agcctgctgt tagaagaatt ggttaa 1836 // ID MT458696; SV 1; linear; other DNA; STD; SYN; 525 BP. XX AC MT458696; XX DT 26-JUN-2020 (Rel. 144, Created) DT 26-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct SARS-CoV-2 assay target sequence. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-525 RA Barra G.B.; RT ; RL Submitted (12-MAY-2020) to the INSDC. RL Research and Development, Sabin Laboratory, SAAN quadra 3 lote 145/185, RL Brasilia, Federal District 70632-340, Brazil XX DR MD5; 0a9bf685e37f2ddcf547b9418aaf04ea. XX CC ##Assembly-Data-START## CC Assembly Method :: Manual v. JAN 2020 CC Sequencing Technology :: Synthetic CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..525 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Severe acute respiratory syndrome FT coronavirus 2 strain Wuhan-Hu-1; gblock- IDT" FT /db_xref="taxon:32630" FT misc_feature 1..525 FT /note="SARS-CoV-2 assay target sequence; dsDNA molecule FT comprising the concatenation of six SARS-CoV-2 sequences FT used as target sequences for the assays E_Sarbeco, FT N_Sarberco, RdRP (Charite protocol) and N1, N2 and N3 (CDC FT protocol) used in the in vitro transcribed for production FT of its RNA form" XX SQ Sequence 525 BP; 138 A; 133 C; 112 G; 142 T; 0 other; taatacgact cactataggg gtgaaatggt catgtgtggc ggttcactat atgttaaacc 60 aggtggaacc tcatcaggag atgccacaac tgcttatgct aatagtgttt ttaacatttg 120 acaggtacgt taatagttaa tagcgtactt ctttttcttg ctttcgtggt attcttgcta 180 gttacactag ccatccttac tgcgcttcga ttgtgtgcgt actgctgcaa tatgacccca 240 aaatcagcga aatgcacccc gcattacgtt tggtggaccc tcagattcaa ctggcagtaa 300 ccagattaca aacattggcc gcaaattgca caatttgccc ccagcgcttc agcgttcttc 360 ggaatgtcgc gcgggagcct tgaatacacc aaaagatcac attggcaccc gcaatcctgc 420 taacaatgct gcaatcgtgc tacaacttcc tcaaggaaca acattgccaa aaggcttcta 480 cgcagaaggg agcagaggcg gcagtcaagc ctcttctcgt tcctc 525 // ID MT559569; SV 1; linear; other DNA; STD; SYN; 433 BP. XX AC MT559569; XX DT 25-JUN-2020 (Rel. 144, Created) DT 25-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct right border T-DNA sequence. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-433 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT "Expression of a Malassezia codon optimized mCherry fluorescent protein in RT a bicistronic vector"; RL Unpublished. XX RN [2] RP 1-433 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT ; RL Submitted (02-JUN-2020) to the INSDC. RL SRIS, Skin Research Institute of Singapore, 11 Mandalay Road, #17-01 RL Clinical Sciences Building, Singapore 308207, Singapore XX DR MD5; e75ee8f0b0df591092ed515f9857d7fc. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..433 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="Agrobacterium tumefaciens EHA105 pAIM2-pPZP201BK FT backbone vector" FT /db_xref="taxon:32630" FT misc_feature 114..138 FT /note="right border T-DNA" XX SQ Sequence 433 BP; 105 A; 111 C; 110 G; 107 T; 0 other; ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc 60 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc 120 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga 180 tccaagctca agctcattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc 240 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt 300 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gccaagcttg 360 gcgcgcctag gcctctgcag gtcgactcta gaggatcccc gggtaccgag ctcgatatct 420 taattaagcc ctt 433 // ID MT559570; SV 1; linear; other DNA; STD; SYN; 977 BP. XX AC MT559570; XX DT 25-JUN-2020 (Rel. 144, Created) DT 25-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct promoter region. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-977 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT "Expression of a Malassezia codon optimized mCherry fluorescent protein in RT a bicistronic vector"; RL Unpublished. XX RN [2] RP 1-977 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT ; RL Submitted (02-JUN-2020) to the INSDC. RL SRIS, Skin Research Institute of Singapore, 11 Mandalay Road, #17-01 RL Clinical Sciences Building, Singapore 308207, Singapore XX DR MD5; 57c7322617f424c8e60a24bc0f20f10d. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..977 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT regulatory 1..971 FT /note="pACT1; actin promoter" FT /regulatory_class="promoter" XX SQ Sequence 977 BP; 237 A; 287 C; 307 G; 146 T; 0 other; tccacggtgc agatcctcgg tgcggagaag gcattgttcc gcgcgctcaa gacgaagggc 60 aacacgccaa agtacggcct gatctatcac gcgtcggcca tatcgcgcgc cgcgcccaag 120 aacaagggcc gcatgtcgcg cttccttgcc aacaagatca gcattgcgtg ccgcattgac 180 tgcttctccg acgcgccctc caccaagttt ggcgaggtcc tgcacatgca ggtcgaggag 240 cgcctcgcct tctacgagac gggcaagcct acgacgaaga atagcgatgc catgcgcaag 300 gcgattgccg ctatggagga ggccgccggc gacctcatgg acgtcgacgc tgacgaggat 360 gctgacgagg acgacgagcc tgctgagccc gccaagccca aggaaaagag caagagcgcc 420 aagaaagaaa agaaggacaa gaaggaaaag aaggacaagt ccaagtccaa atccaaggcg 480 gacgatgtgg aaaaggccgc gaaaaaggcg gccaaaaagg cggccaaaga ggccaaagag 540 gccaagaagg acaagaaaaa gaaggatgcc tgattcagac ctcgatcccc gtagtatgcc 600 gcgcctagac cggccctctt gtataagtaa tagcgatgtt gggatacgac ggcggcagcg 660 cctggggctc ctcgcacacg cctcgcgtgc ctagcgtggc ttgcatgtga tcggcgctgc 720 ctgcgtgcca gcgcagcagt ctcggccgag accacgagcc tgaggccctg tggcggcatt 780 tggagcgtgg cctagtaggt ccccgtggct gtggcatgag cgtccgacag ggggacggga 840 gaggcccgcc ggagtccctg caccgacttt gattggcctt ggctgacaaa gcccgagagg 900 cagcgattgc cagcgatccc gattgtgcga caccgactcg tgttgacgac gcttgtaaca 960 ccaccatcac ccttaag 977 // ID MT559571; SV 1; linear; other DNA; STD; SYN; 714 BP. XX AC MT559571; XX DT 25-JUN-2020 (Rel. 144, Created) DT 25-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct mCherry gene, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-714 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT "Expression of a Malassezia codon optimized mCherry fluorescent protein in RT a bicistronic vector"; RL Unpublished. XX RN [2] RP 1-714 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT ; RL Submitted (02-JUN-2020) to the INSDC. RL SRIS, Skin Research Institute of Singapore, 11 Mandalay Road, #17-01 RL Clinical Sciences Building, Singapore 308207, Singapore XX DR MD5; 8ce9e715836ef65741cfb60827639351. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..714 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS 1..>714 FT /codon_start=1 FT /transl_table=11 FT /product="mCherry" FT /note="Malassezia codon-optimized" FT /protein_id="QKV27546.1" FT /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG FT TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF FT EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK FT GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA FT EGRHSTGGMDELYKLE" XX SQ Sequence 714 BP; 160 A; 187 C; 257 G; 110 T; 0 other; atggtgtcga agggcgagga agacaacatg gcgatcatta aggagttcat gcgctttaag 60 gtgcacatgg agggctcggt gaacggccac gagttcgaga ttgagggcga gggcgagggc 120 cgcccttacg agggcacgca gacggcgaag ctcaaggtga cgaagggcgg ccccctgccc 180 tttgcgtggg acatcctctc gccgcagttt atgtacggct cgaaggcgta cgtgaagcac 240 ccggcggaca ttccggacta cctgaagctc tcgttcccgg agggctttaa gtgggagcgc 300 gtgatgaact ttgaggacgg cggcgtggtg acggtgacgc aggactcgtc gctgcaggac 360 ggcgagttca tctacaaggt gaagctgcgc ggcacgaact ttccctcgga cggcccggtg 420 atgcagaaga agacgatggg ctgggaggcg tcgtcggagc gcatgtaccc cgaggacggc 480 gcgctgaagg gcgagattaa gcagcgcctg aagctcaagg acggtggcca ctacgacgcg 540 gaggtgaaga cgacgtacaa ggcgaagaag cccgtgcagc tgcccggcgc gtacaacgtg 600 aacatcaagc tcgacattac gtcgcacaac gaggactaca cgattgtgga gcagtacgag 660 cgcgcggagg gccgccactc gacgggcggc atggacgagc tctacaagct cgag 714 // ID MT559572; SV 1; linear; other DNA; STD; SYN; 72 BP. XX AC MT559572; XX DT 25-JUN-2020 (Rel. 144, Created) DT 25-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct P2A gene, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-72 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT "Expression of a Malassezia codon optimized mCherry fluorescent protein in RT a bicistronic vector"; RL Unpublished. XX RN [2] RP 1-72 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT ; RL Submitted (02-JUN-2020) to the INSDC. RL SRIS, Skin Research Institute of Singapore, 11 Mandalay Road, #17-01 RL Clinical Sciences Building, Singapore 308207, Singapore XX DR MD5; c76064490484794d8cf1ea0bf35055e5. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..72 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS <1..>72 FT /codon_start=1 FT /transl_table=11 FT /product="P2A" FT /note="Malassezia codon-optimized" FT /protein_id="QKV27547.1" FT /translation="GSGATNFSLLKQAGDVEENPGPTS" XX SQ Sequence 72 BP; 13 A; 23 C; 27 G; 9 T; 0 other; ggctcgggcg cgacgaactt ctcgctcctc aagcaggcgg gcgacgtgga ggagaacccg 60 ggcccgacta gt 72 // ID MT559573; SV 1; linear; other DNA; STD; SYN; 582 BP. XX AC MT559573; XX DT 25-JUN-2020 (Rel. 144, Created) DT 25-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct NAT-resistant marker gene, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-582 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT "Expression of a Malassezia codon optimized mCherry fluorescent protein in RT a bicistronic vector"; RL Unpublished. XX RN [2] RP 1-582 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT ; RL Submitted (02-JUN-2020) to the INSDC. RL SRIS, Skin Research Institute of Singapore, 11 Mandalay Road, #17-01 RL Clinical Sciences Building, Singapore 308207, Singapore XX DR MD5; e3de0b42e5cde3e68b6805a57a9184c2. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..582 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT CDS <1..576 FT /codon_start=1 FT /transl_table=11 FT /product="NAT-resistant marker" FT /note="nourseothricin-resistant marker" FT /protein_id="QKV27548.1" FT /translation="MAAATLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDG FT FTLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWN FT RRLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGF FT TLCGLDTALYDGTASDGEQALYMSMPCP" XX SQ Sequence 582 BP; 140 A; 146 C; 149 G; 147 T; 0 other; atggcggccg ccactcttga cgatacggct taccgttaca gaaccagtgt cccaggtgat 60 gctgaagcca tcgaagcact tgatggtagt ttcacaaccg atactgtctt ccgagtcaca 120 gccaccggtg atggattcac tttgcgagaa gtccctgttg atccacctct tactaaagta 180 tttcctgatg atgaatcaga tgatgaatca gatgcaggtg aagacggcga tccagactcc 240 agaacattcg tcgcttacgg tgacgatgga gaccttgcag gattcgttgt cgtctcttac 300 tccggctgga acagacgtct taccgtcgaa gatatcgaag tcgccccaga acaccgtggt 360 cacggtgtcg gtagagcttt gatgggtctt gctacagaat ttgcacgtga aagaggtgca 420 ggtcacctct ggttggaagt cactaacgtt aacgcacctg ctatccatgc gtaccgacgt 480 atgggtttca ccctttgtgg actggatacc gcactgtacg acggaaccgc atctgacgga 540 gagcaagctc tttatatgag tatgccttgt ccataacaat tg 582 // ID MT559574; SV 1; linear; other DNA; STD; SYN; 492 BP. XX AC MT559574; XX DT 25-JUN-2020 (Rel. 144, Created) DT 25-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct terminator. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-492 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT "Expression of a Malassezia codon optimized mCherry fluorescent protein in RT a bicistronic vector"; RL Unpublished. XX RN [2] RP 1-492 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT ; RL Submitted (02-JUN-2020) to the INSDC. RL SRIS, Skin Research Institute of Singapore, 11 Mandalay Road, #17-01 RL Clinical Sciences Building, Singapore 308207, Singapore XX DR MD5; 58b7e31094d12196a328aa4b3b5a66b6. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..492 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /db_xref="taxon:32630" FT regulatory 1..481 FT /note="tACT1; actin terminator" FT /regulatory_class="terminator" XX SQ Sequence 492 BP; 118 A; 142 C; 137 G; 95 T; 0 other; gttttctaac gatgcggcat ggaatactta gctcttccat agtggccata ctacgatcag 60 tgcccacacg gatcgaggac cgatgtaagt cctattcgcg catgccaaac agcttgccgc 120 cgcgatggcg gacattggcg aacccggccc gggcatccac acagtcgatc gatctgcatt 180 agtcgctgcc aacgcacgtt tcgcgcatcc gggccgacca ggcgatagga cgaagccggg 240 ggaccgttag cgcacacccc agcacgagca ctgcaaaggc caccagggtt tccagtctca 300 tctgacggta agacaggccc acctacatcc aagggaatcg tgttttcgta ctgcgtcgag 360 cctgtgccaa gatctaggct ctttccctgc gataggactg gtgtcaggga actttaccta 420 cactcgtaga tcgagtacgc agcatggaaa aacaggactg ggtcagacat aggagaggac 480 gagggcgaat tc 492 // ID MT559575; SV 1; linear; other DNA; STD; SYN; 335 BP. XX AC MT559575; XX DT 25-JUN-2020 (Rel. 144, Created) DT 25-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct left border T-DNA sequence. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-335 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT "Expression of a Malassezia codon optimized mCherry fluorescent protein in RT a bicistronic vector"; RL Unpublished. XX RN [2] RP 1-335 RA Goh J.P.Z., Ianiri G., Heitman J., Dawson T.L.Jr.; RT ; RL Submitted (02-JUN-2020) to the INSDC. RL SRIS, Skin Research Institute of Singapore, 11 Mandalay Road, #17-01 RL Clinical Sciences Building, Singapore 308207, Singapore XX DR MD5; 932617aa0908ad9f617d4ad1cf176b0a. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..335 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="Agrobacterium tumefaciens EHA105 pAIM2-pPZP201BK FT backbone vector" FT /db_xref="taxon:32630" FT misc_feature 311..>335 FT /note="left border T-DNA" XX SQ Sequence 335 BP; 88 A; 77 C; 79 G; 91 T; 0 other; agggcgaatt cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 60 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 120 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 180 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga 240 gcaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa 300 gcgtcaattt gtttacacca caatatatcc tgcca 335 // ID KX035095; SV 1; circular; other DNA; STD; SYN; 6883 BP. XX AC KX035095; XX DT 19-SEP-2017 (Rel. 134, Created) DT 24-JUN-2020 (Rel. 144, Last updated, Version 2) XX DE Cloning vector pTLR7-A-SEAP, complete sequence. XX KW . XX OS Cloning vector pTLR7-A-SEAP OC other sequences; artificial sequences; vectors. XX RN [1] RC Publication Status: Online-Only RP 1-6883 RX PUBMED; 32554924. RA Azar P., Mejia J.E., Cenac C., Shaiykova A., Youness A., Laffont S., RA Essat A., Izopet J., Passaes C., Muller-Trutwin M., Delobel P., Meyer L., RA Guery J.C.; RT "TLR7 dosage polymorphism shapes interferogenesis and HIV-1 acute viremia RT in women"; RL JCI Insight 5(12):0-136047(2020). XX RN [2] RP 1-6883 RA Mejia J.E., Azar P., Guery J.-C.; RT ; RL Submitted (07-APR-2016) to the INSDC. RL Centre de Physiopathologie de Toulouse-Purpan, Inserm, CHU Purpan, BP 3028, RL Toulouse Cedex 3 31024, France XX DR MD5; 71dc9e4bbc919806866a43e4394f3d88. XX FH Key Location/Qualifiers FH FT source 1..6883 FT /organism="Cloning vector pTLR7-A-SEAP" FT /mol_type="other DNA" FT /db_xref="taxon:1932888" FT misc_feature join(4239..6883,1..688) FT /note="derived from pBC KS+" FT regulatory 1124..2302 FT /note="EEF1A1; derived from human EF-1 alpha constitutive FT promoter and 5'UTR" FT /regulatory_class="promoter" FT 5'UTR 2308..2376 FT /note="derived from human TLR7" FT CDS 2377..3969 FT /codon_start=1 FT /product="TLR7/SEAP fusion protein" FT /note="derived from secreted alkaline phosphatase and human FT toll-like receptor 7 allele A rs179008" FT /protein_id="APT68416.1" FT /translation="MVFPMWTLKRQILILFNIILISKLLGARWFPVEEENPDFWNREAA FT EALGAAKKLQPAQTAAKNLIIFLGDGMGVSTVTAARILKGQKKDKLGPEIPLAMDRFPY FT VALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQCNTTRGNEVISVMNR FT AKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNM FT DIDVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTEL FT MQASLDPSVTHLMGLFEPGDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEG FT GRIDHGHHESRAYRALTETIMFDDAIERAGQLTSEEDTLSLVTADHSHVFSFGGYPLRG FT SSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQQSAVPLDEE FT THAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTDAAHPG FT YSRVGAAGRFEQT" FT regulatory 3961..4182 FT /note="polyadenylation signal from SV40" FT /regulatory_class="other" FT rep_origin 4697..5285 FT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of FT replication" FT CDS complement(5674..6333) FT /codon_start=1 FT /product="chloramphenicol acetyl transferase" FT /note="confers resistance to chloramphenicol" FT /protein_id="APT68415.1" FT /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL FT KTVKKNKHKFYPAFIHILARLMNAHPELRMAMKDGELVIWDSVHPCYTVFHEQTETFSS FT LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM FT DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA" XX SQ Sequence 6883 BP; 1664 A; 1761 C; 1874 G; 1584 T; 0 other; ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc 60 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga 120 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc 180 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc 240 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag 300 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa 360 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac 420 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg 480 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcca 660 ccgcggtggc ggccgctcta gaactagtta gttattacta gccgcgtgca aagcaccggc 720 ggcggtagat gcggggtaag tactgaattt taattcgacc tatcccggta aagcgaaagc 780 gacacgcttt tttttcacac atagcgggac cgaacacgtt ataagtatcg aactcctaaa 840 gaagcagtgt agttttctgc agatgcaaaa aagatctcat gaaggaggaa cttgtgcagt 900 ttacttaatc tgctttaaca cagctaaaag cagagaaaca gtccggaatc tgatggcaaa 960 cacgctaaat gtaagagaag agtgtttgat gctgcagcca gctaaaattc gaggactcag 1020 cgcagctcta ttctggttta gcttgcaaag atggataaag ttttaaacag agaggaatct 1080 ttgcagctaa tggaccttgt aggtcttgaa aggagtggga attggctccg gtgcccgtca 1140 gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg 1200 aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct 1260 ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt 1320 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 1380 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca 1440 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg 1500 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg 1560 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc 1620 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa 1680 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc 1740 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat 1800 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg 1860 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag 1920 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga 1980 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc 2040 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg 2100 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga 2160 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc 2220 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt 2280 tcttccattt caggtgtcgt gaggaattct tggcacctct catgctctgc tctcttcaac 2340 cagacctcta cattccattt tggaagaaga ctaaaaatgg tgtttccaat gtggacactg 2400 aagagacaaa ttcttatcct ttttaacata atcctaattt ccaaactcct tggggctaga 2460 tggtttcctg ttgaggagga gaacccggac ttctggaacc gcgaggcagc cgaggccctg 2520 ggtgccgcca agaagctgca gcctgcacag acagccgcca agaacctcat catcttcctg 2580 ggcgatggga tgggggtgtc tacggtgaca gctgccagga tcctaaaagg gcagaagaag 2640 gacaaactgg ggcctgagat acccctggcc atggaccgct tcccatatgt ggctctgtcc 2700 aagacataca atgtagacaa acatgtgcca gacagtggag ccacagccac ggcctacctg 2760 tgcggggtca agggcaactt ccagaccatt ggcttgagtg cagccgcccg ctttaaccag 2820 tgcaacacga cacgcggcaa cgaggtcatc tccgtgatga atcgggccaa gaaagcaggg 2880 aagtcagtgg gagtggtaac caccacacga gtgcagcacg cctcgccagc cggcacctac 2940 gcccacacgg tgaaccgcaa ctggtactcg gacgccgacg tgcctgcctc ggcccgccag 3000 gaggggtgcc aggacatcgc tacgcagctc atctccaaca tggacattga cgtgatccta 3060 ggtggaggcc gaaagtacat gtttcgcatg ggaaccccag accctgagta cccagatgac 3120 tacagccaag gtgggaccag gctggacggg aagaatctgg tgcaggaatg gctggcgaag 3180 cgccagggtg cccggtatgt gtggaaccgc actgagctca tgcaggcttc cctggacccg 3240 tctgtgaccc atctcatggg tctctttgag cctggagaca tgaaatacga gatccaccga 3300 gactccacac tggacccctc cctgatggag atgacagagg ctgccctgcg cctgctgagc 3360 aggaaccccc gcggcttctt cctcttcgtg gagggtggtc gcatcgacca tggtcatcat 3420 gaaagcaggg cttaccgggc actgactgag acgatcatgt tcgacgacgc cattgagagg 3480 gcgggccagc tcaccagcga ggaggacacg ctgagcctcg tcactgccga ccactcccac 3540 gtcttctcct tcggaggcta ccccctgcga gggagctcca tcttcgggct ggcccctggc 3600 aaggcccggg acaggaaggc ctacacggtc ctcctatacg gaaacggtcc aggctatgtg 3660 ctcaaggacg gcgcccggcc ggatgttacc gagagcgaga gcgggagccc cgagtatcgg 3720 cagcagtcag cagtgcccct ggacgaagag acccacgcag gcgaggacgt ggcggtgttc 3780 gcgcgcggcc cgcaggcgca cctggttcac ggcgtgcagg agcagacctt catagcgcac 3840 gtcatggcct tcgccgcctg cctggagccc tacaccgcct gcgacctggc gccccccgcc 3900 ggcaccaccg acgccgcgca cccgggttac tctagagtcg gggcggccgg ccgcttcgag 3960 cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa 4020 aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca 4080 ataaacaagt taacaacaac aattgcattc attttatgtt tcaggttcag ggggaggtgt 4140 gggaggtttt ttaaagcaag taaaacctct acaaatgtgg taaaatcgat aaggatccgt 4200 cgacctcgag ggggggcccg gtacgcggcc gcaatggcgt acccagcttt tgttcccttt 4260 agtgagggtt aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 4320 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 4380 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 4440 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 4500 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 4560 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 4620 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 4680 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 4740 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 4800 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 4860 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 4920 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 4980 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 5040 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 5100 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 5160 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 5220 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 5280 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 5340 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc ctttttgatc 5400 agaaaaaaag gatcatatcg tcaattatta cctccacggg gagagcctga gcaaactggc 5460 ctcaggcatt tgagaagcac acggtcacac tgcttccggt agtcaataaa ccggtaaacc 5520 agcaatagac ataagcggct atttaacgac cctgccctga accgacgacc gggtcgaatt 5580 tgctttcgaa tttctgccat tcatccgctt attatcactt attcaggcgt agcaaccagg 5640 cgtttaaggg caccaataac tgccttaaaa aaattacgcc ccgccctgcc actcatcgca 5700 gtactgttgt aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg 5760 aacctgaatc gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt 5820 gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact 5880 cacccaggga ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc 5940 caggttttca ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc 6000 gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta 6060 acaagggtga acactatccc atatcaccag ctcaccgtct ttcattgcca tacgtaattc 6120 cggatgagca ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt 6180 atttttcttt acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt 6240 acattgagca actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc 6300 aacggtggta tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct 6360 cgacaactca aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc 6420 tcttacgtgc cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc 6480 aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc 6540 ggtcgaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta 6600 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct 6660 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag 6720 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga 6780 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat 6840 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac 6883 // ID MN750314; SV 1; linear; other DNA; STD; SYN; 1167 BP. XX AC MN750314; XX DT 24-JUN-2020 (Rel. 144, Created) DT 24-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Synthetic construct P2-gD/gC/gB gene, partial cds. XX KW . XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-1167 RX PUBMED; 32002602. RA Wen X., Tong X., Wang M., Wang J., Ni H., Ran X.; RT "Protective immunity following vaccination with a recombinant RT multiple-epitope protein of bovine herpesvirus type I in a rabbit model"; RL Appl Microbiol Biotechnol 104(7):3011-3023(2020). XX RN [2] RP 1-1167 RA Wen X., Ran X.; RT ; RL Submitted (27-NOV-2019) to the INSDC. RL Department of Virus, Heilongjiang Bayi Agricultural University, Daqing, RL Heilongjiang 163319, China XX DR MD5; 5d899a8339472d206754d602adea0287. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..1167 FT /organism="synthetic construct" FT /mol_type="other DNA" FT /note="derived from Bovine alphaherpesvirus 1" FT /db_xref="taxon:32630" FT CDS <1..1167 FT /codon_start=1 FT /transl_table=11 FT /product="P2-gD/gC/gB" FT /protein_id="QKU37011.1" FT /translation="ASGSQYIKANSKFIGITELAAYAAYGSEFGEPKPGPSPDADRPEA FT AYAAYGEPKPGPSPDADRPEAAYAAYGEPKPGPSPDADRPEAAYAAYVVPPYFEESKGY FT EPPPAAAAYAAYVVPPYFEESKGYEPPPAAAAYAAYVVPPYFEESKGYEPPPAAAAYAA FT YLGAARGYTFGACFPARDYEQKKVLEFELAAYAAYAGNASRDGRPSAAYAAYAGNASRD FT GRPSAAYAAYAGNASRDGRPSAAYAAYSAGTTGATPPTPNSPDATPEDSTAAYAAYHRE FT HTSYSPERFQQIEGYYKRAAYAAYHREHTSYSPERFQQIEGYYKRAAYAAYHREHTSYS FT PERFQQIEGYYKRAAYAAYEGLFAAAAPKPGPRRARRAAPAAYAAYVD" XX SQ Sequence 1167 BP; 294 A; 337 C; 298 G; 238 T; 0 other; gctagcggat cccagtatat caaagcaaac agcaaattta tcggcatcac cgaactggca 60 gcatatgcag cctatggatc cgaattcggt gaaccgaaac cgggtccgag tccggatgca 120 gatcgtccgg aagcagcgta tgccgcatat ggcgaaccta aacctggtcc ttcacctgat 180 gcggatcgcc ctgaagctgc ctatgcagcg tacggcgagc caaaaccggg accgtcaccg 240 gatgccgaca gaccggaagc cgcatacgca gcttatgttg ttccgcctta ttttgaagaa 300 agcaaaggtt atgaaccgcc tccggcagca gcagcctacg cagcatacgt tgtgcctccg 360 tatttcgaag aatcaaaagg ctacgaacct ccgcctgcag cagccgcata tgcagcgtat 420 gtggtgcctc cgtactttga agagagtaaa ggatacgagc caccgccagc tgccgcagcg 480 tacgcagcct atctgggtgc agcacgtggt tatacctttg gtgcatgttt tccggcacgt 540 gattatgaac agaaaaaagt tctggaattc gagctcgcag cttatgcagc atatgccggt 600 aatgcaagcc gtgatggtcg tccgagcgca gcatacgcag cctacgcagg taatgcctca 660 cgtgatggcc gaccgtcagc agcgtatgca gcttatgctg gcaacgcgag tcgcgacggt 720 cgtccgtctg cagcttacgc tgcctatagc gcaggcacca ccggtgcaac ccctccgaca 780 ccgaatagcc ctgatgcaac accggaagat agcaccgcag cttacgcagc gtaccatcgt 840 gaacatacca gctatagtcc ggaacgtttt cagcagattg aaggttatta caaacgtgca 900 gcctatgctg cctatcatcg tgaacacacc tcatattcac cggaacgctt ccagcaaatc 960 gagggctact ataaacgtgc tgcttacgca gcatatcacc gcgagcatac cagttactca 1020 cctgagcgtt ttcaacaaat agaagggtat tataagcgtg ctgcctatgc cgcttatgaa 1080 ggtctgtttg cagccgcagc accaaaacca ggtccgcgtc gtgcacgtcg tgccgcacca 1140 gcagcctatg cagcgtatgt cgactaa 1167 // ID MT521917; SV 1; circular; other DNA; STD; SYN; 7054 BP. XX AC MT521917; XX DT 24-JUN-2020 (Rel. 144, Created) DT 24-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Shuttle expression vector pEC-XK99-Gold, complete sequence. XX KW . XX OS Shuttle expression vector pEC-XK99-Gold OC other sequences; artificial sequences; vectors. XX RN [1] RC Publication Status: Online-Only RP 1-7054 RX PUBMED; 32521697. RA Walter T., Al Medani N., Burgardt A., Cankar K., Ferrer L., Kerbs A., RA Lee J.H., Mindt M., Risse J.M., Wendisch V.F.; RT "Fermentative N-Methylanthranilate Production by Engineered Corynebacterium RT glutamicum"; RL Microorganisms 8(6):0-E866(2020). XX RN [2] RP 1-7054 RA Cankar K., Walter T.; RT ; RL Submitted (26-MAY-2020) to the INSDC. RL Chair of Genetics of Prokaryotes, Bielefeld University, RL Universitaetsstrasse 25, Bielefeld, NRW 33615, Germany XX DR MD5; 1767bc007aea1ca8d45d2f972157ed07. XX FH Key Location/Qualifiers FH FT source 1..7054 FT /organism="Shuttle expression vector pEC-XK99-Gold" FT /mol_type="other DNA" FT /note="codon-optimized for expression in Corynebacterium FT glutamicum" FT /db_xref="taxon:2745591" FT regulatory 294..380 FT /note="T1" FT /regulatory_class="terminator" FT regulatory 472..499 FT /note="T2" FT /regulatory_class="terminator" FT gene complement(738..953) FT /gene="per" FT CDS complement(738..953) FT /codon_start=1 FT /transl_table=11 FT /gene="per" FT /product="Per" FT /note="positive effector of replication" FT /protein_id="QKU37006.1" FT /translation="MDLSDVALESDALDAAVDLKTVIGFFRALDTTDAPASRDWASAAS FT DLETLVADLEELADELRARQRQEDAQ" FT gene complement(1596..3062) FT /gene="repA" FT CDS complement(1596..3062) FT /codon_start=1 FT /transl_table=11 FT /gene="repA" FT /product="RepA" FT /protein_id="QKU37007.1" FT /translation="MTLADPQDTVTASAWKLSADLFDTHPEAMRCGSRGWTAEDRRELL FT AHLGRESFQGSKTRDFASAWIKNPDTGETQPKLYRAGSKALTRCQYVALTHAQHAAVIV FT LDIDVPSHQAGGKIEHVNPQVYAILEKWARLEKAPAWIGVNPLSGKCQLIWLIDPVYAA FT AGKTSPNMRLLAATTEEMTRVFGADQAFSHRLSRWPLHVSDDPTAYKWHCQHDRVDRLA FT DLMEIARTMTGSQKPKKYIEQDFSSGRARIEAAQRATAEAKALAILDASLPSALDASGD FT LIDGVRVLWTNPERAARDETAFRHALTVGYQLKAAGERLKDAKIIDAYEVAYNVAQAVG FT ADGREPDLPAMRDRLTMARRVRGYVAKGQPVVPARRVETQSSRGRKALATMGRRGAATS FT NARRWADPESKYAQETRQRLAEANKRREMTGELLELRVKTAILDARSQSVADPSTRELA FT GELGVSERRIQQVRKALGMEAKRGRPRAEN" FT gene 3592..4386 FT /gene="aph(3')-IIa" FT CDS 3592..4386 FT /codon_start=1 FT /transl_table=11 FT /gene="aph(3')-IIa" FT /product="Aph(3')-IIa" FT /note="aminoglycoside phosphotransferase" FT /protein_id="QKU37008.1" FT /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP FT VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS FT SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ FT GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA FT LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" FT rep_origin 4486..5074 FT /note="oriV" FT gene 5664..6746 FT /gene="lacIq" FT CDS 5664..6746 FT /codon_start=1 FT /transl_table=11 FT /gene="lacIq" FT /product="LacIq" FT /note="Lac-repressor" FT /protein_id="QKU37009.1" FT /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL FT NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV FT EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH FT EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA FT MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC FT YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR FT ALADSLMQLARQVSRLESGQ" XX SQ Sequence 7054 BP; 1483 A; 1926 C; 2043 G; 1602 T; 0 other; gaattccaga tgagaccgca tgcctgcaag gtctcagtat gagctcggta cccggggatc 60 ctctagagtc gacctgcagg catgcaagct tggctgtttt ggcggatgag agaagatttt 120 cagcctgata cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg 180 cggcagtagc gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag 240 cgccgatggt agtgtggcgt ctccccatgc gagagtaggg aactgccagg catcaaataa 300 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 360 ctctcctgag taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg 420 gagggtggcg ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca 480 tcctgacgga tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat 540 tcaaatatgt atccgctcat gaattaattc cgctagatga cgtgcggctt cgacctcctg 600 ggcgtggcgc ttgttggcgc gctcgcggct ggctgcggca cgacacgcgt ctgagcagta 660 ttttgcgcgc cgtcctcgtg ggtcaggccg gggtgggatc aggccaccgc agtaggcgca 720 gctgatgcga tcctccacta ctgcgcgtcc tcctggcgct gccgagcacg cagctcgtcg 780 gccagctctt caaggtcggc cacaagcgtt tctaggtcgc tcgcggcact tgcccagtcg 840 cgtgatgctg gcgcgtctgt cgtatcgagg gcgcggaaaa atccgatcac cgtttttaaa 900 tcgacggcgg catcgagtgc gtcggactcc agcgcgacat cggagagatc caccgctgat 960 gcttcaggcc agttttggta cttcgtcgtg aaggtcatga caccattata acgaacgttc 1020 gttaaaaatt ctagccccaa ttctgataat ttcttccggc actcctgcga aaacctgcga 1080 gacttcttgc ccagaaaaaa cgccaagcgc agcggttacc gcactttttt tccaggtgat 1140 ttcaccctga ccagcgaagc ggcactttag tgcatgaggt gtgcccctgg tttcccctct 1200 ttggagggtt caacccaaaa aagcacacaa gcaaaaatga aaatcatcat gagcaagttg 1260 gtgcgaagca gcaacgcgct agctccaaaa acgtctccag gatctcgagg agatttttga 1320 gggggaggga gtcgaggaag agccagagca gaaggcgggg gaaccgttct ctgccgacag 1380 cgtgagcccc cttaaaaatc aggccgggga ggaaccgggg agggatcaga gctaggagcg 1440 agacacccta aaggggggga accgttttct gctgacggtg tttcgtttat tagttttcag 1500 cccgtggata gcggagggtg agggcaagtg agagccagag caaggacggg acccctaaag 1560 gggggaaccg ttttctgctg acggtgtttc gtttattagt tttcagcccg tggacggccg 1620 cgtttagctt ccattccaag tgcctttctg acttgttgga tgcgcctttc actgacacct 1680 agttcgcctg caagctcacg agtcgaggga tcagcaaccg attgagaacg ggcatccagg 1740 atcgcagttt tgacgcgaag ttcgagcaac tcgcctgtca tttctcggcg tttgtttgct 1800 tccgctaatc gctgtcgcgt ctcctgcgca tacttacttt ctgggtcagc ccatctgcgt 1860 gcattcgatg tagctgcgcc ccgtcgcccc atcgtcgcta gagctttccg ccctcggctg 1920 ctctgcgttt ccacccgacg agcagggacg actggctggc ctttagccac gtagccgcgc 1980 acacgacgcg ccatcgtcag gcgatcacgc atggcgggaa gatccggctc ccggccgtct 2040 gcaccgaccg cctgggcaac gttgtacgcc acttcatacg cgtcgatgat cttggcatct 2100 tttaggcgct caccagcagc tttgagctgg tatcccacgg tcaacgcgtg gcgaaacgca 2160 gtctcgtcgc gcgctgcgcg ctctggattt gtccagagca ctcgcacgcc gtcgatcagg 2220 tcgccggacg cgtccagggc gctcggcagg ctcgcgtcca aaatcgctag cgccttggct 2280 tctgcggtgg cgcgttgtgc cgcttcaatg cgggcgcgtc cgctggaaaa gtcctgctca 2340 atgtactttt tcggcttctg tgatccggtc atcgttcgag caatctccat taggtcggcc 2400 agccgatcca cacgatcatg ctggcagtgc catttatagg ctgtcggatc gtctgagacg 2460 tgcagcggcc accggctcag cctatgcgaa aaagcctggt cagcgccgaa aacacgagtc 2520 atttcttccg tcgttgcagc cagcaggcgc atatttgggc tggttttacc tgctgcggca 2580 tacaccgggt caatgagcca gatgagctgg catttcccgc tcagcggatt cacgccgatc 2640 caagccggcg ctttttctag gcgtgcccat ttctctaaaa tcgcgtagac ctgcgggttt 2700 acgtgctcaa tcttcccgcc ggcctggtgg ctgggcacat cgatgtcaag cacgatcacc 2760 gcggcatgtt gcgcgtgcgt cagcgcaacg tactggcacc gcgtcagcgc ttttgagcca 2820 gcccggtaga gctttggttg ggtttcgccg gtatccgggt ttttaatcca ggcgctcgcg 2880 aaatctcttg tcttgctgcc ctggaagctt tcgcgtccca ggtgagcgag cagttcgcgg 2940 cgatcttctg ccgtccagcc gcgtgagccg cagcgcatag cttcggggtg ggtgtcgaac 3000 agatcggcgg acaatttcca cgcgctagct gtgactgtgt cctgcggatc ggctagagtc 3060 atgtcttgag tgctttctcc cagctgatga ctgggggtta gccgacgccc tgtgagttcc 3120 cgctcacggg gcgttcaact ttttcaggta tttgtgcagc ttatcgtgtt ttcttcgtaa 3180 atgaacgctt aactaccttg ttaaacgtgg caaataggca ggattgatgg ggatctagct 3240 tcacgctgcc gcaagcactc agggcgcaag ggctgctaaa ggaagcggaa cacgtagaaa 3300 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca 3360 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag 3420 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct 3480 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga 3540 tggcgcaggg gatcaagatc tgatcaagag acaggatgag gatcgtttcg catgattgaa 3600 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 3660 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 3720 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact ccaagacgag 3780 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 3840 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 3900 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 3960 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 4020 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 4080 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc ggatgcccga cggcgaggat 4140 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 4200 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 4260 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 4320 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 4380 ttctgagcgg gactctgggg ttcgcggaat catgaccaaa atcccttaac gtgagttttc 4440 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt 4500 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt 4560 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat 4620 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc 4680 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa 4740 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg 4800 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag 4860 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag 4920 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa 4980 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt 5040 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg 5100 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc 5160 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac 5220 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct 5280 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga 5340 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg 5400 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc 5460 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca 5520 tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg gcatgcattt 5580 acgttgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag cgcccggaag 5640 agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg 5700 ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga 5760 aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg 5820 cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc 5880 tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca 5940 gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca 6000 atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg 6060 ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc 6120 agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc 6180 atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct 6240 cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga 6300 tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc 6360 tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg 6420 caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat 6480 acgacgatac cgaagacagc tcatgttata tcccgccgtc aaccaccatc aaacaggatt 6540 ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg 6600 tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca 6660 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg 6720 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtaagtta gcgcgaattg 6780 atctggtttg acagcttatc atcgactgca cggtgcacca atgcttctgg cgtcaggcag 6840 ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt cgtgtcgctc 6900 aaggcgcact cccgttctgg ataatgtttt ttgcgccgac atcataacgg ttctggcaaa 6960 tattctgaaa tgagctgttg acaattaatc atccggctcg tataatgtgt ggaattgtga 7020 gcggataaca atttcacaca ggaaacagac catg 7054 // ID MN519522; SV 1; circular; other DNA; STD; SYN; 20300 BP. XX AC MN519522; XX DT 21-JUN-2020 (Rel. 144, Created) DT 21-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning vector OsPHO1pNFG, complete sequence. XX KW . XX OS Cloning vector OsPHO1pNFG OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-20300 RA Reis R.S.; RT "Translation enhancement of rice PHO1.2 by its cis-NAT"; RL Unpublished. XX RN [2] RP 1-20300 RA Reis R.S.; RT ; RL Submitted (28-SEP-2019) to the INSDC. RL Department of Plant Molecular Biology, University of Lausanne, Quartier RL UNIL-Sorge, Batiment Biophore, Lausanne, Vaud 1015, Switzerland XX DR MD5; f5c7ad5534c16462444e62738ed93c3b. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..20300 FT /organism="Cloning vector OsPHO1pNFG" FT /mol_type="other DNA" FT /db_xref="taxon:2744019" FT CDS join(1994..2266,2366..2419,2509..2800,2919..3318, FT 3986..4034,4374..4970) FT /codon_start=1 FT /transl_table=11 FT /product="PHO1spx/nLuc/HA fusion protein" FT /note="PHO1.2 SPX domain" FT /protein_id="QKR72419.1" FT /translation="MVKFSREYEASIIPEWKAAFVDYKRLKKLIKRIKVTRRDDSFAAA FT NAAAAADHLLPPPPAEKEAGGYGFSILDPVRAIAARFSAGQQPSASEDEECPDRGELVR FT STDKHEREFMERADEELEKVNAFYTGQEAELLARGDALLEQLRILADVKRILADHAAAR FT RARGLARSRSMPPPPPSSSPPSSVHGSSGRYLLSGLSSPQSMSDGSLELQQAQVSEGAA FT VADEVMAALERNGVSFVGLAGKKDGKTKDGSGKGRGGGGGGGGGVLQLPATVRIDIPAT FT SPGRAALKVWEELVNVLRKDGADPAAAFVHRKKIQHAEKNIRDAFMALYRGLELLKKFS FT SLNVKAFTKILKKFVKVSEQQRATDLFSGSGSGSMVFTLEDFVGDWRQTAGYNLDQVLE FT QGGVSSLFQNLGVSVTPIQRIVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYP FT VDDHHFKVILHYGTLVIDGVTPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDER FT LINPDGSLLFRVTINGVTGWRLCERILAYPYDVPDYA" FT gene 7065..8717 FT /gene="Fluc" FT CDS 7065..8717 FT /codon_start=1 FT /transl_table=11 FT /gene="Fluc" FT /product="firefly luciferase" FT /protein_id="QKR72420.1" FT /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA FT HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP FT ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS FT MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA FT RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK FT IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY FT GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS FT GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL FT LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV FT FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV" XX SQ Sequence 20300 BP; 5328 A; 4857 C; 5053 G; 5062 T; 0 other; cattctatcc gaccaactca gctcatgtgc atgcatgctt actgtgtaac cgatcagttc 60 atatttatct ttttttttaa aaacacacac acacacacaa ataccatgta ctccactaca 120 attgtagata gataccttag agcagtggtg tagttagaat tttctcacgc ctaaagcaca 180 actagtataa tttacataat ttacctttat ttttcgtgtt ttgaagtttt taaatgtaac 240 ttttaataga tatttaagct tagggcaaca gccctaactg ccctacccag ttttcgtcac 300 tgcgcctaga gaaatatacc ttttttttaa aaaaaaagaa aaacactaaa aagaaagaaa 360 agaaaaagga gtaggagtcg gcgtcatggc cacgtcaggc cggcgtcgct gcagcagcgg 420 cggtggcgtg gcctccattg cgcatacatg gagagcatcc ggctgatctg gtccctgtac 480 tagcagaacc ctagctaagc ttagctccat tgtctccaac ttgcgatcga tccatccatc 540 catccattaa tttccgcgat ccaatcgaac cacgatatgt gtaaagctat agcatatcta 600 cagtcactcc atgctgcacc gcacaaaaca ctctaatcag aattaattaa ttaattaatt 660 atataatcat ccatcctatt ttaaatacat tagcgggttt ccgtgtttgt cgtttgaccg 720 cacatcttat ttaacgaatt tataaaaaaa ataaaaaaaa gtcgcgcata aaatattatt 780 catgttttat catctaatag caacaaaaat actaatcaaa aagtttaaaa caaaacgaac 840 gatcaaactt tagacataga aatccacata catacttaaa agtttacgac cttaattagt 900 cctactcccc tggacactgc cctcttcttt aattaactaa ataattaatt aacacatgag 960 ctgattagtg atactaataa gaaaattata gtgcatgtca actagccaat tacttgatta 1020 gttattgtct ttttttagag gaggcaacta gctaattagc agctgcatgc atggtcacct 1080 gagtgtgagt ggccgagtag gttaagcaag ggtctgtctc tgcttgattc ctgcagctgc 1140 tcatgtttcc ctcactccat tgcccctgcc ttttcgccca aaagatgggc cgctcaaatt 1200 aacaacccgg cctaaagcaa ccggctttct ttcctttctt tctttaattt ctttccttct 1260 cctctttaat tctctgcgct acaaatatgt atacactgcc ttactctaat tagaagtaat 1320 tacttaatca atttctccct taatacagaa ttagcatcca accagatcat gcgacgaggc 1380 ttaattacgt gtacataact agtatataat aatataacta tatatgatcc cactgatcga 1440 attaagcaac taactaagcc actaactgaa tgaacaggac acaaacagtt ctacgaatgg 1500 catggtcgat ccccactatg taagtgtata tatatatctc tgaattcaga tacacctttc 1560 tactttaatt tagctaagct agctagctct acctaatatt gtaacataca aggccactat 1620 ctgtccctgt atgtgcacca gcagtgccaa atttcgctgc atgcttagct aagctaagct 1680 tatatagcag gagcactcga ggtgagcaga gagagaggat tgaagaagaa ggagaagagc 1740 accaagctag tagcctgatc tctctctctc tctctaactg tctatctata taagctaaca 1800 aggcaggacg gttgttgctt gcgagatcat ccctgcctgc ctgtcccttt cctgcatcca 1860 tccatccatc ttgatcttct cctagctcga ttccacacat gtgaacgtac gtacgtttca 1920 gttaattgtt cgatcgatcg atcgatcagc tgctgctgct gctgctgcaa cgaggaagaa 1980 gaagaagaag aagatggtga agttctcgag ggagtacgag gcgagcatca taccggagtg 2040 gaaggctgcg ttcgtcgact acaagcggct caagaagctc atcaagagga tcaaggtcac 2100 ccgccgcgac gactccttcg ccgccgccaa cgccgcagcc gcagccgacc accttctccc 2160 gccgccgccc gcagagaagg aagccggtgg ctatgggttc tccattcttg accccgttcg 2220 cgccatcgcc gcccgcttct ccgctggaca acagccttct gcctcagtac gtcgtcgttg 2280 tgtctcgaat caaatactaa ctaataaacg tgtattaatt aattaataca catttctgat 2340 gttaatacgt atgtgcattt cgcaggagga tgaggagtgc ccggacagag gggagctcgt 2400 gcgatccacg gacaagcatg tataaaatta attagttaat ttgattgtaa ataaaactaa 2460 gtaattaaat tgattgtaaa aatggagatg catggatgga tggtgtagga gagggagttc 2520 atggagaggg cggacgagga gctggagaag gtgaacgcgt tctacacggg gcaggaggcg 2580 gagctgctgg cgcgcggcga cgcgctgctg gagcagctcc ggatcctcgc cgacgtcaag 2640 cgcatcctcg ccgaccacgc cgccgcccgc cgcgcccgcg gcctcgcccg aagccgctct 2700 atgccgccgc cgccgccgtc ctcctcgccg ccgtcgtcgg tgcacggctc cagcggccgc 2760 tacctcctct ccggcctctc ctcgccgcag tccatgtcag gtaggtgaca ccaaccaccc 2820 acacacatga atcctgggct agctgcacat ctctttatgc atgcaatgtc atgtcatgtc 2880 gatgtcgccg tcgtcgtcgt cgatggcgtg ttttgcagat gggagcctgg agttgcagca 2940 ggcgcaggtg tcggagggcg cggcggtggc ggacgaggtg atggcggcgc tggagcggaa 3000 cggcgtcagc ttcgtggggc tcgccgggaa gaaggacggc aagaccaagg acggcagcgg 3060 caaagggcgg ggcggcggcg gcggcggagg cggaggcgtg ctgcagctgc cagcgacggt 3120 gaggatcgac atcccggcga cgagccccgg gcgggcggcg ctcaaggtgt gggaggagct 3180 cgtgaacgtg ctccgcaagg acggcgccga ccccgccgcg gcgttcgtcc accggaagaa 3240 gatccagcac gccgagaaga acatccgcga cgccttcatg gcgctctacc gcggcctcga 3300 gctgctcaag aaattcaggc acatcttccc tcttcatctt tcctttttgc catatctgac 3360 aagctaaaac cacgaagtca cggtatttta gaacaagact tgccttttgt gtctatgtat 3420 attgtcttct atgccatgca tgatgcatat atatacgcat ttgtaagcta tttagaattc 3480 tgatggccaa acctcacact aatagcatgc aactgatcga tcacactaat tgcatgcaca 3540 caaatgatat agatagtttg gttaactgac caactaggaa aaaaaaatta agttgtttct 3600 tcatagatgt atatctaaca aattactact atgaatatta gttgcagcat gagtcatgga 3660 gcactgtgtg agcagcacac aactacacct gcactgcacc caagcatgca gtacagtagt 3720 agtttagtca aatttcaacc ccgttttgca tggaagcatc cgtgtaaatc acgtagccat 3780 ccatacacca tcctgtgtca cctgaaagcg actgtttctc ctttcaaatt actaattctt 3840 ttgtggtttt tgaaaaggta gattaattca ccggttattt taggtggctg tctttatatt 3900 tcttttactt gtcaattatg gtgttcttgt ggttctgacg gatttggatg cttttttggt 3960 tgcatgcatg gatggatggg agcagctctc tcaatgtaaa ggctttcacc aaaatattga 4020 agaaatttgt caaggtaata tactaattag ccacatctgt gcccttttga aatggatgga 4080 tggaagaatg aagagtctaa ataatagatg ataaagatta aagaaaaccg gtctacatat 4140 ataaggcttc taggagaata aagttctaag tgtactatta gtagtttagt accctcaaag 4200 cagaggtact agatctcatt ctcgacagtg taatcctagt cactagtcag cctaataaca 4260 tgagacagca taaacatttt cttgcaagga ataatttaat tttttaatat atttttttaa 4320 aaaaatgcta ctccgagctg acagtgactg gtacgaactg ctttgcattt caggtgtccg 4380 agcaacagcg agctacagac ctgttttcag gtagcggcag cggtagcatg gtcttcacac 4440 tcgaagattt cgttggggac tggcgacaga cagccggcta caacctggac caagtccttg 4500 aacagggagg tgtgtccagt ttgtttcaga atctcggggt gtccgtaact ccgatccaaa 4560 ggattgtcct gagcggtgaa aatgggctga agatcgacat ccatgtcatc atcccgtatg 4620 aaggtctgag cggcgaccaa atgggccaga tcgaaaaaat ttttaaggtg gtgtaccctg 4680 tggatgatca tcactttaag gtgatcctgc actatggcac actggtaatc gacggggtta 4740 cgccgaacat gatcgactat ttcggacggc cgtatgaagg catcgccgtg ttcgacggca 4800 aaaagatcac tgtaacaggg accctgtgga acggcaacaa aattatcgac gagcgcctga 4860 tcaaccccga cggctccctg ctgttccgag taaccatcaa cggagtgacc ggctggcggc 4920 tgtgcgaacg cattctggcg tacccatacg atgttccaga ttacgcttga ccttagtaaa 4980 agggtgggat atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc 5040 ttaagtttgt gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt 5100 aaatgaatgt aagatctcat tataatgaat aaacaaatgt ttctataatc cattgtgaat 5160 gttttgttgg atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg 5220 aatatgatta aagataaggc gcgccgaccc agctttcttg tacaaagtgg tgatatcact 5280 agtgtatgta ggtatagtaa catgaacaag aacagactca agtacaagat cgccaggatt 5340 cgggtcaagg cggaagccag cgcgccaccc cacgtcagca aatacggagg cgcggggttg 5400 acggcgtcac ccggtcctaa cggcgaccaa caaaccagcc agaagaaatt acagtaaaaa 5460 aaaagtaaat tgcactttga tccacctttt attacctaag tctcaatttg gatcaccctt 5520 aaacctatct tttcaatttg ggccgggttg tggtttggac taccatgaac aacttttcgt 5580 catgtctaac ttccctttca gcaaacatat gaaccatata tagaggagat cggccgtata 5640 ctagagctga tgtgtttaag gtcgttgatt gcacgagaaa aaaaaatcca aatcgcaaca 5700 atagcaaatt tatctggttc aaagtgaaaa gatatgttta aaggtagtcc aaagtaaaac 5760 ttatagataa taaaatgtgg tccaaagcgt aattcactca aaaaaaatca acgagacgtg 5820 taccaaacgg agacaaacgg catcttctcg aaatttccca accgctcgct cgcccgcctc 5880 gtcttcccgg aaaccgcggt ggtttcagcg tggcggattc tccaagcaga cggagacgtc 5940 acggcacggg actcctccca ccacccaacc gccataaata ccagccccct catctcctct 6000 cctcgcatca gctccacccc cgaaaaattt ctccccaatc tcgcgaggct ctcgtcgtcg 6060 aatcgaatcc tctcgcgtcc tcaaggtacg ctgcttctcc tctcctcgct tcgtttcgat 6120 tcgatttcgg acgggtgagg ttgttttgtt gctagatccg attggtggtt agggttgtcg 6180 atgtgattat cgtgagatgt ttaggggttg tagatctgat ggttgtgatt tgggcacggt 6240 tggttcgata ggtggaatcg tggttaggtt ttgggattgg atgttggttc tgatgattgg 6300 ggggaatttt tacggttaga tgaattgttg gatgattcga ttggggaaat cggtgtagat 6360 ctgttgggga attgtggaac tagtcatgcc tgagtgattg gtgcgatttg tagcgtgttc 6420 catcttgtag gccttgttgc gagcatgttc agatctactg ttccgctctt gattgagtta 6480 ttggtgccat gggttggtgc aaacacaggc tttaatatgt tatatctgtt ttgtgtttga 6540 tgtagatctg tagggtagtt cttcttagac atggttcaat tatgtagctt gtgcgtttcg 6600 atttgatttc atatgttcac agattagata atgatgaact cttttaatta attgtcaatg 6660 gtaaatagga agtcttgtcg ctatatctgt cataatgatc tcatgttact atctgccagt 6720 aatttatgct aagaactata ttagaatatc atgttacaat ctgtagtaat atcatgttac 6780 aatctgtagt tcatctatat aatctattgt ggtaatttct ttttactatc tgtgtgaaga 6840 ttattgccac tagttcattc tacttatttc tgaagttcag gatacgtgtg ctgttactac 6900 ctatctgaat acatgtgtga tgtgcctgtt actatctttt tgaatacatg tatgttctgt 6960 tggaatatgt ttgctgtttg atccgttgtt gtgtccttaa tcttgtgcta gttcttaccc 7020 tatctgtttg gtgattattt cttgcagatt cagatcgcta gcccatggaa gacgccaaaa 7080 acataaagaa aggcccggcg ccattctatc cgctggaaga tggaaccgct ggagagcaac 7140 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac 7200 atatcgaggt ggacatcact tacgctgagt acttcgaaat gtccgttcgg ttggcagaag 7260 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc 7320 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga 7380 acgacattta taatgaacgt gaattgctca acagtatggg catttcgcag cctaccgtgg 7440 tgttcgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaag ctcccaatca 7500 tccaaaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca 7560 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgtg ccagagtcct 7620 tcgataggga caagacaatt gcactgatca tgaactcctc tggatctact ggtctgccta 7680 aaggtgtcgc tctgcctcat agaactgcct gcgtgagatt ctcgcatgcc agagatccta 7740 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg 7800 gttttggaat gtttactaca ctcggatatt tgatatgtgg atttcgagtc gtcttaatgt 7860 atagatttga agaagagctg tttctgagga gccttcagga ttacaagatt caaagtgcgc 7920 tgctggtgcc aaccctattc tccttcttcg ccaaaagcac tctgattgac aaatacgatt 7980 tatctaattt acacgaaatt gcttctggtg gcgctcccct ctctaaggaa gtcggggaag 8040 cggttgccaa gaggttccat ctgccaggta tcaggcaagg atatgggctc actgagacta 8100 catcagctat tctgattaca cccgaggggg atgataaacc gggcgcggtc ggtaaagttg 8160 ttccattttt tgaagcgaag gttgtggatc tggataccgg gaaaacgctg ggcgttaatc 8220 aaagaggcga actgtgtgtg agaggtccta tgattatgtc cggttatgta aacaatccgg 8280 aagcgaccaa cgccttgatt gacaaggatg gatggctaca ttctggagac atagcttact 8340 gggacgaaga cgaacacttc ttcatcgttg accgcctgaa gtctctgatt aagtacaaag 8400 gctatcaggt ggctcccgct gaattggaat ccatcttgct ccaacacccc aacatcttcg 8460 acgcaggtgt cgcaggtctt cccgacgatg acgccggtga acttcccgcc gccgttgttg 8520 ttttggagca cggaaagacg atgacggaaa aagagatcgt ggattacgtc gccagtcaag 8580 taacaaccgc gaaaaagttg cgcggaggag ttgtgtttgt ggacgaagta ccgaaaggtc 8640 ttaccggaaa actcgacgca agaaaaatca gagagatcct cataaaggcc aagaagggcg 8700 gaaagatcgc cgtgtaatga gcttggaatg gatcttcgat cccgatcgtt caaacatttg 8760 gcaataaagt ttcttaagat tgaatcctgt tgccggtctt gcgacgatta tcatataatt 8820 tctgttgaat tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag 8880 atgggttttt atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat 8940 atagcgcgca aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga 9000 attgccaagc taattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta 9060 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg 9120 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat 9180 aaccctgata aatgcttcaa taatgggacc gactcgcgct gtcaggagca ccggtcaaca 9240 tggtggagca cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc 9300 aaagggcaat tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt 9360 gcccagctat ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat 9420 gccatcattg cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca 9480 aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt 9540 caaagcaagt ggattgatgt gataacatgg tggagcacga cacacttgtc tactccaaaa 9600 atatcaaaga tacagtctca gaagaccaaa gggcaattga gacttttcaa caaagggtaa 9660 tatccggaaa cctcctcgga ttccattgcc cagctatctg tcactttatt gtgaagatag 9720 tggaaaagga aggtggctcc tacaaatgcc atcattgcga taaaggaaag gccatcgttg 9780 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg 9840 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg 9900 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa 9960 gttcatttca tttggagagg acctcgactc tagaggatcc cccgagctcg aattatcaca 10020 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat 10080 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta 10140 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt 10200 ggattttgag ttaggatccg gcgagatttt caggagctaa ggaagctaaa atggagaaaa 10260 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg 10320 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct 10380 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg 10440 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga 10500 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat 10560 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg 10620 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt 10680 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg 10740 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc 10800 tgatgccgct ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa 10860 tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa acgcgtggat 10920 ccggcttact aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa 10980 gaatatatac tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg 11040 tattacagtg acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat 11100 atctccggtc tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc 11160 tggaaagcgg aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc 11220 tcttttgctg acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag 11280 agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg 11340 acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact 11400 ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag 11460 tgtgccggtg tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat 11520 caaaaacgcc attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag 11580 ccagtctgca ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct 11640 gttttttatg caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt 11700 cagctttctt gtacaaagtg gtgatagctt ggcgccctta gtaaaagggt gggatatgaa 11760 gatgaagatg aaatatttgg tgtgtcaaat aaaaagcttg tgtgcttaag tttgtgtttt 11820 tttcttggct tgttgtgtta tgaatttgtg gctttttcta atattaaatg aatgtaagat 11880 ctcattataa tgaataaaca aatgtttcta taatccattg tgaatgtttt gttggatctc 11940 ttctgcagca tataactact gtatgtgcta tggtatggac tatggaatat gattaaagat 12000 aaggcgcgcc gacccagctt tcttgtacaa agtggtgata tcactagtgt atgtaggtat 12060 agtaacatga acaagaacag actcaagtac aagatcgcca tgatcataca tgagaattaa 12120 gggagtcacg ttatgacccc gccgatgacg cgggacaagc cgttttacgt ttggaactga 12180 cagaaccgca acgttgaagg agccactgag ccgcgggttt ctggagttta atgagctaag 12240 cacatacgtc agaaaccatt attgcgcgtt caaaagtcgc ctaaggtcac tatcagctag 12300 caaatatttc ttgtcaaaaa tgctccactg acgttccata aattcccctc ggtatccaat 12360 tagagtctca tattcactct caactcgaat cccccctatg aaaaagcctg aactcaccgc 12420 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct 12480 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct 12540 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc 12600 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagttcagcg agagcctgac 12660 ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact 12720 gcccgctgtt cttcagccgg tcgcggaggc tatggatgct atcgctgcgg ccgatcttag 12780 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg 12840 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga 12900 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg cgaggactgc 12960 cccgaagtcc ggcactcgtg cacgcggatt cggctccaac aatgtcctga cggacaatgg 13020 ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt 13080 cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 13140 cgagcggagg catccggagc ttgcaggatc gccaaggctc cgggcgtata tgctccgcat 13200 tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 13260 gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat 13320 cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg 13380 aaaccgacgc cccagcactc gtccgagggc aaaggaatag agtagatgcc gaccgaacaa 13440 gagctgattt cgagaacgcc tcagccagca actcgcgcga gcctagcaag gcaaatgcga 13500 gagaacggcc ttacgcttgg tggcacagtt ctcgtccaca gttcgctaag ctcgctcggc 13560 tgggtcgcgg gagggccggt cgcagtgatt caggaattaa ttccctagag tcaagcagat 13620 cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg tcttgcgatg 13680 attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat gtaatgcatg 13740 acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat ttaatacgcg 13800 atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt gtcatctatg 13860 ttactagatc gaccggcatg caagctgata attcaattcg gtggacatga gacgaattct 13920 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata 13980 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag 14040 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc 14100 ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc ctaagttacg 14160 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata 14220 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct 14280 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc 14340 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc 14400 gaccgcccgg agctggccag gatgcttgac cacctacgcc ctggcgacgt tgtgacagtg 14460 accaggctag accgcctggc ccgcagcacc cgcgacctac tggacattgc cgagcgcatc 14520 caggaggccg gcgcgggcct gcgtagcctg gcagagccgt gggccgacac caccacgccg 14580 gccggccgca tggtgttgac cgtgttcgcc ggcattgccg agttcgagcg ttccctaatc 14640 atcgaccgca cccggagcgg gcgcgaggcc gccaaggccc gaggcgtgaa gtttggcccc 14700 cgccctaccc tcaccccggc acagatcgcg cacgcccgcg agctgatcga ccaggaaggc 14760 cgcaccgtga aagaggcggc tgcactgctt ggcgtgcatc gctcgaccct gtaccgcgca 14820 cttgagcgca gcgaggaagt gacgcccacc gaggccaggc ggcgcggtgc cttccgtgag 14880 gacgcattga ccgaggccga cgccctggcg gccgccgaga atgaacgcca agaggaacaa 14940 gcatgaaacc gcaccaggac ggccaggacg aaccgttttt cattaccgaa gagatcgagg 15000 cggagatgat cgcggccggg tacgtgttcg agccgcccgc gcacctctca accgtgcggc 15060 tgcatgaaat cctggccggt ttgtctgatg ccaagctggc ggcctggccg gccagcttgg 15120 ccgctgaaga aaccgagcgc cgccgtctaa aaaggtgatg tgtatttgag taaaacagct 15180 tgcgtcatgc ggtcgctgcg tatatgatcc gatgagtaaa taaacaaata cgcaagggga 15240 acgcatgaag gttatcgctg tacttaacca gaaaggcggg tcaggcaaga cgaccatcgg 15300 aacccatcta gcccgcgccc tgcaactcgc cggggccgat gttctgttag tcgattccga 15360 tccccagggc agtgcccgcg attgggcggc cgtgcgggaa gatcaaccgc taaccgttgt 15420 cggcatcgac cgcccgacga ttgaccgcga cgtgaaggcc atcggccggc gcgacttcgt 15480 agtgatcgac ggagcgcccc aggcggcgga cttggctgtg tccgcgatca aggcagccga 15540 cttcgtgctg attccggtgc agccaagccc ttacgacata tgggccaccg ccgacctggt 15600 ggagctggtt aagcagcgca ttgaggtcac ggatggaagg ctacaagcgg cctttgtcgt 15660 gtcgcgggcg atcaaaggca cgcgcatcgg cggtgaggtt gccgaggcgc tggccgggta 15720 cgagctgccc attcttgagt cccgtatcac gcagcgcgtg agctacccag gcactgccgc 15780 cgccggcaca accgttcttg aatcagaacc cgagggcgac gctgcccgcg aggtccaggc 15840 gctggccgct gaaattaaat caaaactcat ttgagttaat gaggtaaaga gaaaatgagc 15900 aaaagcacaa acacgctaag tgccggccgt ccgagcgcac gcagcagcaa ggctgcaacg 15960 ttggccagcc tggcagacac gccagccatg aagcgggtca actttcagtt gccggcggag 16020 gatcacacca agctgaagat gtacgcggta cgccaaggca agaccattac cgagctgcta 16080 tctgaataga tcgcgcagct accagagtaa atgagcaaat gaataaatga gtagatgaat 16140 tttagcggct aaaggaggcg gcatggaaaa tcaagaacaa ccaggcaccg acgccgtgga 16200 atgccccatg tgtggaggaa cgggcggttg gccaggcgta agcggctggg ttgtctgccg 16260 gccctgcaat ggcactggaa cccccaagcc cgaggaatcg gcgtgacggt cgcaaaccat 16320 ccggcccggt acaaatcggc gcggcgctgg gtgatgacct ggtggagaag ttgaaggccg 16380 cgcaggccgc ccagcggcaa cgcatcgagg cagaagcacg ccccggtgaa tcgtggcaag 16440 cggccgctga tcgaatccgc aaagaatccc ggcaaccgcc ggcagccggt gcgccgtcga 16500 ttaggaagcc gcccaagggc gacgagcaac cagatttttt cgttccgatg ctctatgacg 16560 tgggcacccg cgatagtcgc agcatcatgg acgtggccgt tttccgtctg tcgaagcgtg 16620 accgacgagc tggcgaggtg atccgctacg agcttccaga cgggcacgta gaggtttccg 16680 cagggccggc cggcatggcc agtgtgtggg attacgacct ggtactgatg gcggtttccc 16740 atctaaccga atccatgaac cgataccggg aagggaaggg agacaagccc ggccgcgtgt 16800 tccgtccaca cgttgcggac gtactcaagt tctgccggcg agccgatggc ggaaagcaga 16860 aagacgacct ggtagaaacc tgcattcggt taaacaccac gcacgttgcc atgcagcgta 16920 cgaagaaggc caagaacggc cgcctggtga cggtatccga gggtgaagcc ttgattagcc 16980 gctacaagat cgtaaagagc gaaaccgggc ggccggagta catcgagatc gagctagctg 17040 attggatgta ccgcgagatc acagaaggca agaacccgga cgtgctgacg gttcaccccg 17100 attacttttt gatcgatccc ggcatcggcc gttttctcta ccgcctggca cgccgcgccg 17160 caggcaaggc agaagccaga tggttgttca agacgatcta cgaacgcagt ggcagcgccg 17220 gagagttcaa gaagttctgt ttcaccgtgc gcaagctgat cgggtcaaat gacctgccgg 17280 agtacgattt gaaggaggag gcggggcagg ctggcccgat cctagtcatg cgctaccgca 17340 acctgatcga gggcgaagca tccgccggtt cctaatgtac ggagcagatg ctagggcaaa 17400 ttgccctagc aggggaaaaa ggtcgaaaag gactctttcc tgtggatagc acgtacattg 17460 ggaacccaaa gccgtacatt gggaaccgga acccgtacat tgggaaccca aagccgtaca 17520 ttgggaaccg gtcacacatg taagtgactg atataaaaga gaaaaaaggc gatttttccg 17580 cctaaaactc tttaaaactt attaaaactc ttaaaacccg cctggcctgt gcataactgt 17640 ctggccagcg cacagccgaa gagctgcaaa aagcgcctac ccttcggtcg ctgcgctccc 17700 tacgccccgc cgcttcgcgt cggcctatcg cggccgctgg ccgctcaaaa atggctggcc 17760 tacggccagg caatctacca gggcgcggac aagccgcgcc gtcgccactc gaccgccggc 17820 gcccacatca aggcaccctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 17880 atgcagctcc cggtgacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 17940 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt 18000 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag 18060 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 18120 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 18180 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 18240 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 18300 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 18360 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 18420 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 18480 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 18540 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 18600 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 18660 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 18720 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 18780 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 18840 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 18900 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 18960 tggtcatgca ttctaggtga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta 19020 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa 19080 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact 19140 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag 19200 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagtttatg catttctttc 19260 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa 19320 ccgttattca ttcgtgattg cgcctgagcg agtcgaaata cgcgatcgct gttaaaagga 19380 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata 19440 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc tgggatcgca 19500 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc 19560 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta 19620 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcggtagatt 19680 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc 19740 atgttggaat ttaatcgcgg ccttgagcaa gacgtttccc gttgaatatg gctcataaca 19800 gaacttatta tttccttcct cttttctaca gtatttaaag ataccccaag aagctaatta 19860 taacaagacg aactccaatt cactgttcct tgcattctaa aaccttaaat accagaaaac 19920 agctttttca aagttgtttt caaagttggc gtataacata gtatcgacgg agccgatttt 19980 gaaaccgcgg tgatcacagg cagcaacgct ctgtcatcgt tacaatcaac atgctaccct 20040 ccgcgagatc atccgtgttt caaacccggc agcttagttg ccgttcttcc gaatagcatc 20100 ggtaacatga gcaaagtctg ccgccttaca acggctctcc cgctgacgcc gtcccggact 20160 gatgggctgc ctgtatcgag tggtgatttt gtgccgagct gccggtcggg gagctgttgg 20220 ctggctggtg gcaggatata ttgtggtgta aacataacga attcgtctca ggagatacat 20280 gagaattaag ggagtcacgg 20300 // ID MN528127; SV 1; circular; other DNA; STD; SYN; 8159 BP. XX AC MN528127; XX DT 21-JUN-2020 (Rel. 144, Created) DT 21-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE UNVERIFIED: Cloning vector pCW619-LM dal, complete sequence. XX KW UNVERIFIED. XX OS Cloning vector pCW619-LM dal OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-8159 RA Yuzhen Z.; RT ; RL Submitted (02-OCT-2019) to the INSDC. RL Department of Public Health Laboratory Sciences, West China School of RL Public HealthSichuan University, No. 17, Section 3, Renmin South Road, RL Wuhou District, Chengdu, Sichuan 610041, China XX DR MD5; d2fae96f251a25c761eb35ab2958be4c. XX CC GenBank staff is unable to verify sequence and/or annotation CC provided by the submitter. CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..8159 FT /organism="Cloning vector pCW619-LM dal" FT /mol_type="other DNA" FT /db_xref="taxon:2741709" XX SQ Sequence 8159 BP; 2604 A; 1579 C; 1750 G; 2226 T; 0 other; tcatgtttga cagcttatca tcgtcgggca gcgttgggtc ctggccacgg gtgcgcatga 60 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga 120 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct 180 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt 240 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg 300 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt 360 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt 420 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca 480 tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct 540 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct 600 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta 660 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 720 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 780 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg 840 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 900 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 960 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1020 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 1080 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 1140 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 1200 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 1260 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 1320 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 1380 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 1440 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 1500 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 1560 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 1620 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 1680 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 1740 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 1800 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 1860 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 1920 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 1980 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 2040 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 2100 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 2160 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg 2220 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 2280 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 2340 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 2400 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 2460 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 2520 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 2580 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 2640 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 2700 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 2760 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 2820 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 2880 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 2940 aggccctttc gtcttcaaga attcccagca tggagccaca caactgtcgg cggccttcca 3000 attactgaat ggattagcga agatgaacaa ggtgcaatgg atactatttt cgtaagtgtt 3060 cgtgatgcag cttatgaaat tattaataaa aaaggcgcta cattctacgg cgttgctgca 3120 gctcttgctc gtattacaaa agcaattcta aataacgaaa atgcgatttt gccactttct 3180 gtttatttag atggccatta cggtatgaac gatatttata taggtgcacc agcagtcgtt 3240 aaccgtcaag gcgttcgcca tattgttgaa atgaacttaa atgacaaaga aaaagaacaa 3300 atgaaaaact ccgcagatac acttaaaaaa gttctagaca ttatcgctgc cacacatgat 3360 gcgttaattc atttattagc agaaacaacg acagaaccat tcgcgggaca accgcttacg 3420 acccttgcga ccgtttcgtt agcatataat gaacaagacg taccgatttt acctgatgga 3480 acaggctatc tagttgccag aaccgcacca tataaaacca ctgcttgtac atgggtgcag 3540 aaaaaatggc cgcatatggt accaaagaac aaaatgttac tacgaggctt tgttgggaaa 3600 gcaggagaaa cttggttaga gcaagcaagc gatgaagcaa tcgtctcagc tgttttgaga 3660 gattatgcgg aaattatgga ccttcacgcc gcgccacttt tttatgaagt aagccgaatg 3720 aaatccgcga tgcctcaata cttagttaat caccaggacc gactaaaaca attgaaaaag 3780 aacatcaaaa cagattaccc aggtgtttat cttgcaggaa tgagctacga aggtgtgggt 3840 attcctgatt gcatcgcagg ggctcaaaca gccgctaagg aattagtaga ttacttggaa 3900 gaggtgtaac atgattaaag gaattggtct agatatgatt gatttagaac gtgtaaaaca 3960 agtcgtggaa aagaatccgc gctttatcga acgcgtttta acagaaaaag aaattaagca 4020 atttgaaaaa tacgaaggta atcgcaaaat cgaattttta gctgggcgtt ttgcagcaaa 4080 agaagcatat gctaaggcga atggaactgg ttttggcaaa catttaagtt ttacggatgt 4140 cgaaattttg caagtagaag atggtcgccc ccatgttact ttaccagtca aatcaggcga 4200 aaacgttttt gttagcatca cccacacagc tagatcagcc gcagctcaag tgataattga 4260 aatttagaaa ggaaagtgaa caatcaaggt aatattcgtg ctttttttag aagaagcgtt 4320 tataatctaa catatgaaac tttatagcta tatataggac ttaagggagt atatcagcta 4380 gaactgtaat attcttaaca ataaaatgcg aaataactct tttcttcaag gcgtgataat 4440 ggtacgatat agttgttact tacaggtacg aggctttggg ggtgtgacac gtgttagaga 4500 aagaaaatcg gatgataata tccgtagaac tgacacagga aatgatacaa gaactcgacg 4560 tagttgtaga aaaagaaaaa atggggcgga gtgaagttat aatggaagca acgcaacagt 4620 ttttacaaga gaaaagggct cgcgaattaa gagacgagat ggaacgcggc tatgcagaga 4680 tggcgacaat taattttgct atcgcatgcg agtgtaccca tgtcgaagca gaagcagaag 4740 acaggaatat tagtatttta ggaggttaat ggctgatggt gaagcgtggt gacgtatact 4800 acgcggacct ttcccccgtg gtcggaagcg agcaaggggg aatacggcct gttctcatca 4860 ttcaaaatga tattggtaat agattcagtc caactgtgat cgtggcagca ataactgcaa 4920 aaattcaaaa agcaaaattg ccaacgcacg tggaagctac tcgtaaagat ggctttgaga 4980 gagattctgt cattctttta gaacagatta gaacgattga taaacagcgc ctgacagata 5040 aaattacgca tttggacgaa gatttaatgg ccaaggtaaa caaggcactt gaagtcagtc 5100 taggagtagt agaattttaa tccatcctaa aaaaaataac ataaaatgat gaatgcaggg 5160 caaaagggtc tgacgtaaac tagtcccggg gcgaattgca tctgaccgaa ttttacgttt 5220 ccctgaataa ttctcatcaa tcgtttcatc aattttatct ttatacttta tattttgtgc 5280 gttaatcaaa tcataatttt tatatgtttc ctcatgattt atgtctttat tattatagtt 5340 tttattctct ctttgattat gtctttgtat cccgtttgta ttacttgatc ctttaactct 5400 ggcaaccctc aaaattgaat gagacatgct acacctccgg ataataaata tatataaacg 5460 tataaagatt tcataaagtc taacacacta gacttattta cttcgtaatt aagtcgttaa 5520 accgtgtgct ctacgaccaa aactataaaa cctttaagaa ctttcttttt ttacaagaaa 5580 aaagaaatta gataaatctc tcatatcttt tattcaataa tcgcatccga ttgcagtata 5640 aatttaacga tcactcatca tgttcatatt tatcagagct cgtgctataa ttatactaat 5700 tttataagga ggaaaaaata tgggcatttt tagtattttt gtaatcagca cagttcatta 5760 tcaaccaaac aaaaaataag tggttataat gaatcgttaa taagcaaaat tcatataacc 5820 aaattaaaga gggttataat gaacgagaaa aatataaaac acagtcaaaa ctttattact 5880 tcaaaacata atatagataa aataatgaca aatataagat taaatgaaca tgataatatc 5940 tttgaaatcg gctcaggaaa aggccatttt acccttgaat tagtaaagag gtgtaatttc 6000 gtaactgcca ttgaaataga ccataaatta tgcaaaacta cagaaaataa acttgttgat 6060 cacgataatt tccaagtttt aaacaaggat atattgcagt ttaaatttcc taaaaaccaa 6120 tcctataaaa tatatggtaa tataccttat aacataagta cggatataat acgcaaaatt 6180 gttcttgata gtatagctaa tgagatttat ttaatcgtgg aatacgggtt tgctaaaaga 6240 ttattaaata caaaacgctc attggcatta cttttaatgg cagaagttga tatttctata 6300 ttaagtatgg ttccaagaga atattttcat cctaaaccta aagtgaatag ctcacttatc 6360 agattaagta gaaaaaaatc aagaatatca cacaaagata aacaaaagta taattatttc 6420 gttctgaaat gggttaacaa agaatacaag aaaatattta caaaaaatca atttaacaat 6480 tccttaaaac atgcaggaat tgacgattta aacaatatta gctttgaaca attcttatct 6540 cttgtcaata gctataaatt atttaataag taagttaagg gatgcataaa ctgcatccct 6600 taacttgttt ttcgtgtgcc tattttttgt gaatcgtcga caagctttaa tgcggtagtt 6660 tatcacagtt aaattgctaa cgcagtcagg caccgtgtat gaaatctaac aatgcgctca 6720 tcgtcatcct cggcaccgtc accctggatg ctgtaggcat aggcttggtt atgccggtac 6780 tgccgggcct cttgcgggat cgatagaaag cgtgagaaac agcgtacaga cgatttagag 6840 atgtagaggt acttttatgc cgagaaaact ttttgcgtgt gacagtcctt aaaatatact 6900 tagagcgtaa gcgaaagtag tagcgacagc tattaacttt cggttgcaaa gctctaggat 6960 ttttaatgga cgcagcgcat cacacgcaaa aaggaaattg gaataaatgc gaaatttgag 7020 atgttaatta aagacctttt tgaggtcttt ttttcttaga tttttggggt tatttagggg 7080 agaaaacata ggggggtact acgacctccc ccctaggtgt ccattgtcca ttgtccaaac 7140 aaataaataa atattgggtt tttaatgtta aaaggttgtt ttttatgtta aagtgaaaaa 7200 aacagatgtt gggaggtaca gtgatagttg tagatagaaa agaagagaaa aaagttgctg 7260 ttactttaag acttacaaca gaagaaaatg agatattaaa tagaatcaaa gaaaaatata 7320 atattagcaa atcagatgca accggtattc taataaaaaa atatgcaaag gaggaatacg 7380 gtgcatttta aacaaaaaaa gatagacagc actggcatgc tgcctatcta tgactaaatt 7440 ttgttaagtg tattagcacc gttattatat catgagcgaa aatgtaataa aagaaactga 7500 aaacaagaaa aattcaagag gacgtaattg gacatttgtt ttatatccag aatcagcaaa 7560 gccgagtggt tagagtattt aaaagagtta cacattcaat ttgtagtgct ccattacatg 7620 atagggatac tgatacagaa ggtaggatga aaaaagagca ttatcatatt ctagtgatgt 7680 atgagggtaa taaatcttat gaacagataa aaataataac agaagaattg aatgcgacta 7740 ttccgcagat tgcaggaagt gtgaaaggtc ttgtgagata tatgcttcac atggacgatc 7800 ctaataaatt taaatatcaa aaagaagata tgatagttta tggcggtgta gatgttgatg 7860 aattattaaa gaaaacaaca acagatagat ataaattaat taaagaaatg attgagttta 7920 ttgatgaaca aggaatcgta gaatttaaga gtttaatgga ttatgcaatg aagtttaaat 7980 ttgatgattg gttcccgctt ttatgtgata actcggcgta tgttattcaa gaatatataa 8040 aatcaaatcg gtataaatct gaccgataga ttttgaattt aggtgtcaca agacactctt 8100 ttttcgcacc agcgaaaact ggtttaagcc gagtgcgcaa aagacataat cgggaattc 8159 // ID MN528128; SV 1; circular; other DNA; STD; SYN; 8201 BP. XX AC MN528128; XX DT 21-JUN-2020 (Rel. 144, Created) DT 21-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE UNVERIFIED: Cloning vector pCW619-LM dat, complete sequence. XX KW UNVERIFIED. XX OS Cloning vector pCW619-LM dat OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-8201 RA Yuzhen Z.; RT ; RL Submitted (02-OCT-2019) to the INSDC. RL Department of Public Health Laboratory Sciences, West China School of RL Public HealthSichuan University, No. 17, Section 3, Renmin South Road, RL Wuhou District, Chengdu, Sichuan 610041, China XX DR MD5; 6d6c0c0caac65199cb6f9e18b89cee30. XX CC GenBank staff is unable to verify sequence and/or annotation CC provided by the submitter. CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..8201 FT /organism="Cloning vector pCW619-LM dat" FT /mol_type="other DNA" FT /db_xref="taxon:2741710" XX SQ Sequence 8201 BP; 2496 A; 1661 C; 1641 G; 2403 T; 0 other; tcatgtttga cagcttatca tcgtcgggca gcgttgggtc ctggccacgg gtgcgcatga 60 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga 120 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct 180 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt 240 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg 300 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt 360 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt 420 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca 480 tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct 540 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct 600 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta 660 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 720 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 780 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg 840 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 900 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 960 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1020 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 1080 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 1140 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 1200 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 1260 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 1320 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 1380 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 1440 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 1500 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 1560 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 1620 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 1680 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 1740 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 1800 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 1860 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 1920 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 1980 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 2040 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 2100 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 2160 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg 2220 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 2280 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 2340 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 2400 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 2460 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 2520 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 2580 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 2640 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 2700 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 2760 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 2820 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 2880 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 2940 aggccctttc gtcttcaaga attcccagca tggagccaca caactgtcgg cggccttcca 3000 attactgaat ggattagcga agatgaacaa ggtgcaatgg atactatttt cgtaagtgtt 3060 cgtgatgcag cttatgaaat tattaataaa aaaggcgcta cattctacgg cgttgctgca 3120 gctcttgctc gtattacaaa agcaattcta aataacgaaa atgcgatttt gccactttct 3180 gtttatttag atggccatta cggtatgaac gatatttata taggtgcacc agcagtcgtt 3240 aaccgtcaag gcgttcgcca tattgttgaa atgaacttaa atgacaaaga aaaagaacaa 3300 atgaaaaact ccgcagatac acttaaaaaa gttctagagt aatcatcgcg aataagtaaa 3360 gctgacgcgt tgtaaatcgt tcaattaaaa aggccgtcat tggaatcatg acaccattaa 3420 ctaacataaa tccagttgat aaccattgtc cttgactggc tgtaattcca aaatctttca 3480 taatacttgg tagcgcgaca ttcatcaacg tttggttgag aatggtaaca aaggcgccca 3540 taagcataac aatcaaaata ccgttacgct tcactgatgt acttgctgct ttcatattca 3600 atctcgtgca ccttcccttt ccaaaatggt aataatttct tggtgcattt ttagcatttc 3660 ttttaaccga tcctcgccaa tctctaagat aggttctaaa cggttgaaga atacatgata 3720 cgtttctgct gcttttgtag ctcctttttc agttaattct aagctgagtg cgcgacgatt 3780 attttcattt cttgtacgct ttaaaaatcc cgcttttact aatcgatcta caataccaga 3840 tacagtactt ttaccaagct tcattcgttc tgctaattcg ccaagtgtta attctgattc 3900 tctttgtaat tctctgattg caagtaattg ggttgtagta atctctcggt tcgcagcttc 3960 ttcagctagc acgtgatgtg tttttcgttg tacttcgcga aaagagaaaa tgacctcttt 4020 caccaatttt tcatccatga cttccctcca tattgttcgc tctcaaatat ttcgtatgcg 4080 aactattagc cttttgaatt atacaccctg taaaaaattt gtcaatcgct ttaaatggca 4140 cttttaagta tgaaagcgac accaattgag tattttcaac gaataaaaaa gttatataaa 4200 tttttacata ggtatcaaag cacaaaaaac cttcctgaaa taggaaggtt ttctactatg 4260 ctcgagtctc tccttgcata gttaattttt ggaactatct aaaaacttaa tgaaacaaag 4320 aaactgccgc aaaactaagc tttgcagcag ttaatttttt caattataac ccttacttcg 4380 ctaatttgta aagcgcttct gcgtaaattg ctgtagcttt taacaaatca tcaaaatagc 4440 tgaattcgtc tttttggtgc atcgtgtctt cacggcctgg gaatagcgct ccgaacgcta 4500 cgccagtctc catatgacgt gcataagttc cgccacctat tgctagtaaa gtagcctctt 4560 cacctgtttg ttttgtatat acttcttgta aaacttgaat aagtgggtga tcttttggta 4620 cgaaaagtgg tttggaatct tcataatgtg tatattgcgc attatactca tatacgactg 4680 tttgcatttt atttttaagt ttgtccatgc tagcagtaac tggataacgg aaattaagtc 4740 catatttgcc accttctgca acatcataac ggataacgcc aacgttcatt gttagttcgc 4800 cgctttctgc atcttcatag ctaatgccta gtttaacagc acgagaatca ccaaacagat 4860 agtcacgacc aaatgttacg aaatcattcg ctgctccagt cagcttgaat ttaccaagga 4920 aagcgactaa atgtagacca gcgttcacac cgttatttgg ttccattgca tgggcagatt 4980 tgccgacaat attaatttta acggacttgc cattttcttc tagtgttcct tctactggat 5040 gatttgctag gaaggttttg aaggcactcg ctactttatc aaagtctttt acatcttcta 5100 aaatagctgt tgcgtgatct ggaaccatgt tgtagcgttc gcctgattct acgctaagta 5160 aacggaatgc tgcttccccg cctgcttcac cgtctttaaa tgatacatct agttctgaga 5220 actagtcccg gggcgaattg catctgaccg aattttacgt ttccctgaat aattctcatc 5280 aatcgtttca tcaattttat ctttatactt tatattttgt gcgttaatca aatcataatt 5340 tttatatgtt tcctcatgat ttatgtcttt attattatag tttttattct ctctttgatt 5400 atgtctttgt atcccgtttg tattacttga tcctttaact ctggcaaccc tcaaaattga 5460 atgagacatg ctacacctcc ggataataaa tatatataaa cgtataaaga tttcataaag 5520 tctaacacac tagacttatt tacttcgtaa ttaagtcgtt aaaccgtgtg ctctacgacc 5580 aaaactataa aacctttaag aactttcttt ttttacaaga aaaaagaaat tagataaatc 5640 tctcatatct tttattcaat aatcgcatcc gattgcagta taaatttaac gatcactcat 5700 catgttcata tttatcagag ctcgtgctat aattatacta attttataag gaggaaaaaa 5760 tatgggcatt tttagtattt ttgtaatcag cacagttcat tatcaaccaa acaaaaaata 5820 agtggttata atgaatcgtt aataagcaaa attcatataa ccaaattaaa gagggttata 5880 atgaacgaga aaaatataaa acacagtcaa aactttatta cttcaaaaca taatatagat 5940 aaaataatga caaatataag attaaatgaa catgataata tctttgaaat cggctcagga 6000 aaaggccatt ttacccttga attagtaaag aggtgtaatt tcgtaactgc cattgaaata 6060 gaccataaat tatgcaaaac tacagaaaat aaacttgttg atcacgataa tttccaagtt 6120 ttaaacaagg atatattgca gtttaaattt cctaaaaacc aatcctataa aatatatggt 6180 aatatacctt ataacataag tacggatata atacgcaaaa ttgttcttga tagtatagct 6240 aatgagattt atttaatcgt ggaatacggg tttgctaaaa gattattaaa tacaaaacgc 6300 tcattggcat tacttttaat ggcagaagtt gatatttcta tattaagtat ggttccaaga 6360 gaatattttc atcctaaacc taaagtgaat agctcactta tcagattaag tagaaaaaaa 6420 tcaagaatat cacacaaaga taaacaaaag tataattatt tcgttctgaa atgggttaac 6480 aaagaataca agaaaatatt tacaaaaaat caatttaaca attccttaaa acatgcagga 6540 attgacgatt taaacaatat tagctttgaa caattcttat ctcttgtcaa tagctataaa 6600 ttatttaata agtaagttaa gggatgcata aactgcatcc cttaacttgt ttttcgtgtg 6660 cctatttttt gtgaatcgtc gacaagcttt aatgcggtag tttatcacag ttaaattgct 6720 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg 6780 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg 6840 atcgatagaa agcgtgagaa acagcgtaca gacgatttag agatgtagag gtacttttat 6900 gccgagaaaa ctttttgcgt gtgacagtcc ttaaaatata cttagagcgt aagcgaaagt 6960 agtagcgaca gctattaact ttcggttgca aagctctagg atttttaatg gacgcagcgc 7020 atcacacgca aaaaggaaat tggaataaat gcgaaatttg agatgttaat taaagacctt 7080 tttgaggtct ttttttctta gatttttggg gttatttagg ggagaaaaca taggggggta 7140 ctacgacctc ccccctaggt gtccattgtc cattgtccaa acaaataaat aaatattggg 7200 tttttaatgt taaaaggttg ttttttatgt taaagtgaaa aaaacagatg ttgggaggta 7260 cagtgatagt tgtagataga aaagaagaga aaaaagttgc tgttacttta agacttacaa 7320 cagaagaaaa tgagatatta aatagaatca aagaaaaata taatattagc aaatcagatg 7380 caaccggtat tctaataaaa aaatatgcaa aggaggaata cggtgcattt taaacaaaaa 7440 aagatagaca gcactggcat gctgcctatc tatgactaaa ttttgttaag tgtattagca 7500 ccgttattat atcatgagcg aaaatgtaat aaaagaaact gaaaacaaga aaaattcaag 7560 aggacgtaat tggacatttg ttttatatcc agaatcagca aagccgagtg gttagagtat 7620 ttaaaagagt tacacattca atttgtagtg ctccattaca tgatagggat actgatacag 7680 aaggtaggat gaaaaaagag cattatcata ttctagtgat gtatgagggt aataaatctt 7740 atgaacagat aaaaataata acagaagaat tgaatgcgac tattccgcag attgcaggaa 7800 gtgtgaaagg tcttgtgaga tatatgcttc acatggacga tcctaataaa tttaaatatc 7860 aaaaagaaga tatgatagtt tatggcggtg tagatgttga tgaattatta aagaaaacaa 7920 caacagatag atataaatta attaaagaaa tgattgagtt tattgatgaa caaggaatcg 7980 tagaatttaa gagtttaatg gattatgcaa tgaagtttaa atttgatgat tggttcccgc 8040 ttttatgtga taactcggcg tatgttattc aagaatatat aaaatcaaat cggtataaat 8100 ctgaccgata gattttgaat ttaggtgtca caagacactc ttttttcgca ccagcgaaaa 8160 ctggtttaag ccgagtgcgc aaaagacata atcgggaatt c 8201 // ID MN528129; SV 1; circular; other DNA; STD; SYN; 8713 BP. XX AC MN528129; XX DT 21-JUN-2020 (Rel. 144, Created) DT 21-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE UNVERIFIED: Cloning vector pCW619-LI dal, complete sequence. XX KW UNVERIFIED. XX OS Cloning vector pCW619-LI dal OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-8713 RA Yuzhen Z.; RT ; RL Submitted (02-OCT-2019) to the INSDC. RL Department of Public Health Laboratory Sciences, West China School of RL Public HealthSichuan University, No. 17, Section 3, Renmin South Road, RL Wuhou District, Chengdu, Sichuan 610041, China XX DR MD5; 096586c44387b63689af0151762688ae. XX CC GenBank staff is unable to verify sequence and/or annotation CC provided by the submitter. CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..8713 FT /organism="Cloning vector pCW619-LI dal" FT /mol_type="other DNA" FT /db_xref="taxon:2741707" XX SQ Sequence 8713 BP; 2795 A; 1647 C; 1874 G; 2397 T; 0 other; tcatgtttga cagcttatca tcgtcgggca gcgttgggtc ctggccacgg gtgcgcatga 60 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga 120 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct 180 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt 240 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg 300 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt 360 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt 420 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca 480 tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct 540 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct 600 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta 660 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 720 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 780 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg 840 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 900 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 960 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1020 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 1080 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 1140 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 1200 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 1260 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 1320 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 1380 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 1440 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 1500 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 1560 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 1620 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 1680 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 1740 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 1800 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 1860 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 1920 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 1980 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 2040 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 2100 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 2160 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg 2220 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 2280 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 2340 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 2400 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 2460 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 2520 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 2580 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 2640 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 2700 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 2760 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 2820 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 2880 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 2940 aggccctttc gtcttcaaga attcccagca tggagccaca caactgtcgg cggccttcca 3000 attactgaat ggattagcga agatgaacaa ggtgcaatgg atactatttt cgtaagtgtt 3060 cgtgatgcag cttatgaaat tattaataaa aaaggcgcta cattctacgg cgttgctgca 3120 gctcttgctc gtattacaaa agcaattcta aataacgaaa atgcgatttt gccactttct 3180 gtttatttag atggccatta cggtatgaac gatatttata taggtgcacc agcagtcgtt 3240 aaccgtcaag gcgttcgcca tattgttgaa atgaacttaa atgacaaaga aaaagaacaa 3300 atgaaaaact ccgcagatac acttaaaaaa gttctagagc ttatagagcc acttttatct 3360 ggtatttatg caggagatat ttataaaatg agcttgcggg caacatttcc gcaatttgaa 3420 gaagcagtgg ctaaacacgg tagtttgatg gaaggcctgc aacaaaacca gctgaacacg 3480 accggaacca aagcaactat tggtgcattc aggacgctag atggcgggct agaagcttta 3540 ccaaaagcag ttgaacaagc tcttcctaag aaccatttgc acacaggaaa acaagcaacg 3600 caaattttga aaaaaggtca ttcttatgaa attttttttg aaaatggaga taaaatatct 3660 gctgatggcg tcattattgc tgctactcac gatacattaa ttgatttact tggagccagg 3720 gtaacagaac cgtttgccaa ccagccatta accagccttg caacagtctc attagcttat 3780 aatgaaagtg atgtacccat tttaccggat ggaacgggtt atttagttgc tagaactgct 3840 ccttatcgaa ccactgcttg tacatgggtg caaaaaaaat ggccacatat ggtacctgaa 3900 aataaaatgc tactacgagg ttttgttggt aaagcaggtg aaacttggtt agaagaggca 3960 agtgatgaag caattatttc tgctgtttta gcagattatg ccgaaatgat ggatatcgaa 4020 agagcgccac ttttttatga agtaagtcgg atgaagtctg caatgccaca gtatttagtg 4080 acacaccaag ctagactaaa acaactaaaa gaaaatattg cgacagacta cccgggaatt 4140 tattttgcag gaatgagtta tcaaggtgtt ggaattcctg attgtattgc tggggcgaaa 4200 ctagctgtta atgacgttac tgattttttg aaagaggtgt aagatgatta aaggtattgg 4260 gcttgatatg attgatttag cacgagtaaa acaagtttta gaaaagaatc cccgttttat 4320 ggagcggatt ttaacagaaa aagaaattag ccaatttgaa aaatatgaag gtagtcgtaa 4380 aattgaattt ttagcggggc gttttgctgc caaagaagct tatgcaaaag cgaacggaac 4440 tggatttgga aaacatttaa gctttacgga tgtagaaatt ttacaagtag aagatggcag 4500 acctcacgtg acaatgccga tcaaacctgg tgagtcggtg tttgtgagta ttacccacac 4560 ggcgagatct gctgcggcac aagtgattat tgaagtttag aaaggaatgt ggaaattaag 4620 gtaatattcg tgcttttttg aggagaagcg tttataatct aacatatgaa actttatagc 4680 tacatctagg acttaaggga ggatgttgga taaaactata acaatcttaa caagtaaatg 4740 ctaaataact cttttcttca tggcttgata atggtacgat atagttgtta cttacaggta 4800 cgaggctttg ggggtgtgac acgtgttaga gaaagaaaag cggatgataa tatccgtaga 4860 actgacacaa gaaatggtac aggaactcga cgtagttgta gaaaaagaaa aaatggggcg 4920 gagtgaagtt ataatggaag caacgcaaca atttttgcaa gagaaaaggg cacgtgagtt 4980 gagagacgag atggagcgcg gctatgcgga aatggcgaca attaactttg ctatcgcatg 5040 cgagtgtacc catgtcgaat cagaagcaga agacagaaat attagtattt taggaggtta 5100 atggctgatg gtgaagcgtg gtgacgtata ctacgcggac ctttcccccg tggtcggaag 5160 cgagcaaggg ggaatacggc ctgttctcat cattcaaaac gacattggta atagattcag 5220 cccaactgtg attgtggcag caataaccgc aaagattcaa aaagcaaagc tgccaacaca 5280 tgtggaagct actcgtaaag atggctttga aagagattct gtcattcttt tagaacaaat 5340 tagaacaatt gacaaacaac gcctgacaga taagattaca catttggacg aggaactaat 5400 ggtcaaggta aaccaggcgc tggaagtcag tctaggagta gtagaattct aaacaaccta 5460 aaaatataac atagaatgat gaacgcagga tagtaaataa gtccgacgta gaagaatacc 5520 aaatacataa tgaactaatt aaccacttta caagatttaa gggacatcag aaacctacaa 5580 acaatgcgta tgattcatcc ttcgctaaag ttgtttctaa tgtctttttt taaaaagtag 5640 ttaatcaaat cgcagaaaaa aagagagatc tttaaggtgg agaaaggttg gaaaataatc 5700 aatgtataaa gattttgcaa acttcatccg gaactagtcc cggggcgaat tgcatctgac 5760 cgaattttac gtttccctga ataattctca tcaatcgttt catcaatttt atctttatac 5820 tttatatttt gtgcgttaat caaatcataa tttttatatg tttcctcatg atttatgtct 5880 ttattattat agtttttatt ctctctttga ttatgtcttt gtatcccgtt tgtattactt 5940 gatcctttaa ctctggcaac cctcaaaatt gaatgagaca tgctacacct ccggataata 6000 aatatatata aacgtataaa gatttcataa agtctaacac actagactta tttacttcgt 6060 aattaagtcg ttaaaccgtg tgctctacga ccaaaactat aaaaccttta agaactttct 6120 ttttttacaa gaaaaaagaa attagataaa tctctcatat cttttattca ataatcgcat 6180 ccgattgcag tataaattta acgatcactc atcatgttca tatttatcag agctcgtgct 6240 ataattatac taattttata aggaggaaaa aatatgggca tttttagtat ttttgtaatc 6300 agcacagttc attatcaacc aaacaaaaaa taagtggtta taatgaatcg ttaataagca 6360 aaattcatat aaccaaatta aagagggtta taatgaacga gaaaaatata aaacacagtc 6420 aaaactttat tacttcaaaa cataatatag ataaaataat gacaaatata agattaaatg 6480 aacatgataa tatctttgaa atcggctcag gaaaaggcca ttttaccctt gaattagtaa 6540 agaggtgtaa tttcgtaact gccattgaaa tagaccataa attatgcaaa actacagaaa 6600 ataaacttgt tgatcacgat aatttccaag ttttaaacaa ggatatattg cagtttaaat 6660 ttcctaaaaa ccaatcctat aaaatatatg gtaatatacc ttataacata agtacggata 6720 taatacgcaa aattgttctt gatagtatag ctaatgagat ttatttaatc gtggaatacg 6780 ggtttgctaa aagattatta aatacaaaac gctcattggc attactttta atggcagaag 6840 ttgatatttc tatattaagt atggttccaa gagaatattt tcatcctaaa cctaaagtga 6900 atagctcact tatcagatta agtagaaaaa aatcaagaat atcacacaaa gataaacaaa 6960 agtataatta tttcgttctg aaatgggtta acaaagaata caagaaaata tttacaaaaa 7020 atcaatttaa caattcctta aaacatgcag gaattgacga tttaaacaat attagctttg 7080 aacaattctt atctcttgtc aatagctata aattatttaa taagtaagtt aagggatgca 7140 taaactgcat cccttaactt gtttttcgtg tgcctatttt ttgtgaatcg tcgacaagct 7200 ttaatgcggt agtttatcac agttaaattg ctaacgcagt caggcaccgt gtatgaaatc 7260 taacaatgcg ctcatcgtca tcctcggcac cgtcaccctg gatgctgtag gcataggctt 7320 ggttatgccg gtactgccgg gcctcttgcg ggatcgatag aaagcgtgag aaacagcgta 7380 cagacgattt agagatgtag aggtactttt atgccgagaa aactttttgc gtgtgacagt 7440 ccttaaaata tacttagagc gtaagcgaaa gtagtagcga cagctattaa ctttcggttg 7500 caaagctcta ggatttttaa tggacgcagc gcatcacacg caaaaaggaa attggaataa 7560 atgcgaaatt tgagatgtta attaaagacc tttttgaggt ctttttttct tagatttttg 7620 gggttattta ggggagaaaa catagggggg tactacgacc tcccccctag gtgtccattg 7680 tccattgtcc aaacaaataa ataaatattg ggtttttaat gttaaaaggt tgttttttat 7740 gttaaagtga aaaaaacaga tgttgggagg tacagtgata gttgtagata gaaaagaaga 7800 gaaaaaagtt gctgttactt taagacttac aacagaagaa aatgagatat taaatagaat 7860 caaagaaaaa tataatatta gcaaatcaga tgcaaccggt attctaataa aaaaatatgc 7920 aaaggaggaa tacggtgcat tttaaacaaa aaaagataga cagcactggc atgctgccta 7980 tctatgacta aattttgtta agtgtattag caccgttatt atatcatgag cgaaaatgta 8040 ataaaagaaa ctgaaaacaa gaaaaattca agaggacgta attggacatt tgttttatat 8100 ccagaatcag caaagccgag tggttagagt atttaaaaga gttacacatt caatttgtag 8160 tgctccatta catgataggg atactgatac agaaggtagg atgaaaaaag agcattatca 8220 tattctagtg atgtatgagg gtaataaatc ttatgaacag ataaaaataa taacagaaga 8280 attgaatgcg actattccgc agattgcagg aagtgtgaaa ggtcttgtga gatatatgct 8340 tcacatggac gatcctaata aatttaaata tcaaaaagaa gatatgatag tttatggcgg 8400 tgtagatgtt gatgaattat taaagaaaac aacaacagat agatataaat taattaaaga 8460 aatgattgag tttattgatg aacaaggaat cgtagaattt aagagtttaa tggattatgc 8520 aatgaagttt aaatttgatg attggttccc gcttttatgt gataactcgg cgtatgttat 8580 tcaagaatat ataaaatcaa atcggtataa atctgaccga tagattttga atttaggtgt 8640 cacaagacac tcttttttcg caccagcgaa aactggttta agccgagtgc gcaaaagaca 8700 taatcgggaa ttc 8713 // ID MN528130; SV 1; circular; other DNA; STD; SYN; 8118 BP. XX AC MN528130; XX DT 21-JUN-2020 (Rel. 144, Created) DT 21-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE UNVERIFIED: Cloning vector pCW619-LI dat, complete sequence. XX KW UNVERIFIED. XX OS Cloning vector pCW619-LI dat OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-8118 RA Yuzhen Z.; RT ; RL Submitted (02-OCT-2019) to the INSDC. RL Department of Public Health Laboratory Sciences, West China School of RL Public HealthSichuan University, No. 17, Section 3, Renmin South Road, RL Wuhou District, Chengdu, Sichuan 610041, China XX DR MD5; d72afa8589fc3d15391bc122e8ef5ef3. XX CC GenBank staff is unable to verify sequence and/or annotation CC provided by the submitter. CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..8118 FT /organism="Cloning vector pCW619-LI dat" FT /mol_type="other DNA" FT /db_xref="taxon:2741708" XX SQ Sequence 8118 BP; 2438 A; 1663 C; 1644 G; 2373 T; 0 other; tcatgtttga cagcttatca tcgtcgggca gcgttgggtc ctggccacgg gtgcgcatga 60 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga 120 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct 180 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt 240 cagcgccctg caccattatg ttccggatct gcatcgcagg atgctgctgg ctaccctgtg 300 gaacacctac atctgtatta acgaagcgct ggcattgacc ctgagtgatt tttctctggt 360 cccgccgcat ccataccgcc agttgtttac cctcacaacg ttccagtaac cgggcatgtt 420 catcatcagt aacccgtatc gtgagcatcc tctctcgttt catcggtatc attaccccca 480 tgaacagaaa ttccccctta cacggaggca tcaagtgacc aaacaggaaa aaaccgccct 540 taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac tcaacgagct 600 ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg atgagcttta 660 ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 720 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc 780 gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg 840 agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg 900 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct 960 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 1020 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 1080 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 1140 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 1200 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 1260 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 1320 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 1380 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 1440 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 1500 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 1560 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 1620 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 1680 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 1740 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 1800 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 1860 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 1920 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 1980 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 2040 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 2100 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 2160 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg 2220 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 2280 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 2340 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 2400 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 2460 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat 2520 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 2580 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 2640 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 2700 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 2760 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 2820 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 2880 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 2940 aggccctttc gtcttcaaga attcccagca tggagccaca caactgtcgg cggccttcca 3000 attactgaat ggattagcga agatgaacaa ggtgcaatgg atactatttt cgtaagtgtt 3060 cgtgatgcag cttatgaaat tattaataaa aaaggcgcta cattctacgg cgttgctgca 3120 gctcttgctc gtattacaaa agcaattcta aataacgaaa atgcgatttt gccactttct 3180 gtttatttag atggccatta cggtatgaac gatatttata taggtgcacc agcagtcgtt 3240 aaccgtcaag gcgttcgcca tattgttgaa atgaacttaa atgacaaaga aaaagaacaa 3300 atgaaaaact ccgcagatac acttaaaaaa gttctagaag taaggcaaat cctcctcgga 3360 gcacgcgttc ctggtcgctt agttctcggc cggatatcca atcttttcca gtaactgctg 3420 aagaggcttc tagtgcgcca aatcctactc caagtgctaa tccggctggt gggcagacga 3480 tacttacgcc cacaatcacc acggcggcaa cgatattgac ccagaactct ttctcttctt 3540 gttcgtcttg gacggattta taatcaaacg cacgagtatg taacatcccc attttaaaat 3600 cggcttcgct aaccttttgg tccggatttt catgcttcca gacttcgtat tggagggtca 3660 tttggttggc gtagaagtta tcgccattca ttaagtcctc aatagttgca ttttctttta 3720 ccgattcaat cacttggggc tgattactgt acgaagacat atacgtttgt gtcgacttag 3780 ccccaatacg gtttgtagtt gagtatgtcg tgatactcaa gtcttgcatc ccatctacaa 3840 atgcatccaa cttcttgtaa aaaggctcat caatcagcgt ttcgattttc tcccctgcgt 3900 gacaactaaa ctgatacagc acctttaagt cttcctcgat ttccccgtac ttgcttcgat 3960 ggtcggtatg ttggaaatga atcgcgtgat cggtgtctaa gtcacggata tcgtcttccg 4020 cattctccat atttttcgac actttttcta aatgattaaa taggccacca aaccaattgg 4080 ctgcattaaa tggagctagg gcacttttgg ctttttacca gtcgtcttct cggtagttta 4140 cgtctttcat tcatctgtcc tcgactttct ttgttatatg agctgtatat taatgaaagt 4200 tcatctccaa atcttgttat atgtttcttt taatcaatat aagcaaccat ccttttaaga 4260 tggttgccca aacctcaaga cactccttgc tagttaattt attggaacta tctaaaaact 4320 taattaaata gagaaactgc cgcaaaacta agctttgcag cagttaattt tttcaattat 4380 aacccttact ttgctaattt gtaaagcgct tctgcgtaaa ttgctgtcgc ttttaacaaa 4440 tcgtcaaaat aactgaattc gtctttttgg tgcatcgtat cttcgcggcc tgggaatagt 4500 gcgccaaacg ccacacctgt ttccatatga cgtgcataag ttccgccacc aattgcaagt 4560 aaagtagctt cttctcctgt ttgtttcgta taaacttctt gtaaagtttg aattaatggg 4620 tggtcttttg gtacgaaaag cggtttggaa tcactgtaat gtgtgtactg tgcattatat 4680 tcatatacga cagtttgcat tttatttttc agcttatcca tgtttgcagt aactgggtaa 4740 cggaaattaa gtccgtattt cccaccttcc ccaacatcat aacggataac gccaacattc 4800 atcgttaatt cgccactttc tttatcttcg tagctaatac caagttttac agcacgagaa 4860 tcgccaaata agtaatcacg accaaatgta acgaaatcat tagcagcacc agttaattta 4920 aatttaccta agaaggctac taaatgaaga cctgcattga taccattatt tggttccatt 4980 gcatgagcag attttccaac catgttaatt ttaactgttt taccgtcttc ttctaacgta 5040 ccttctactg gatggttagc taagaaagtt ttgaaagtac ttgttagttt atcgtagtct 5100 ttcacgtttt cgatgatggc actagcatga tctggtaact agtcccgggg cgaattgcat 5160 ctgaccgaat tttacgtttc cctgaataat tctcatcaat cgtttcatca attttatctt 5220 tatactttat attttgtgcg ttaatcaaat cataattttt atatgtttcc tcatgattta 5280 tgtctttatt attatagttt ttattctctc tttgattatg tctttgtatc ccgtttgtat 5340 tacttgatcc tttaactctg gcaaccctca aaattgaatg agacatgcta cacctccgga 5400 taataaatat atataaacgt ataaagattt cataaagtct aacacactag acttatttac 5460 ttcgtaatta agtcgttaaa ccgtgtgctc tacgaccaaa actataaaac ctttaagaac 5520 tttctttttt tacaagaaaa aagaaattag ataaatctct catatctttt attcaataat 5580 cgcatccgat tgcagtataa atttaacgat cactcatcat gttcatattt atcagagctc 5640 gtgctataat tatactaatt ttataaggag gaaaaaatat gggcattttt agtatttttg 5700 taatcagcac agttcattat caaccaaaca aaaaataagt ggttataatg aatcgttaat 5760 aagcaaaatt catataacca aattaaagag ggttataatg aacgagaaaa atataaaaca 5820 cagtcaaaac tttattactt caaaacataa tatagataaa ataatgacaa atataagatt 5880 aaatgaacat gataatatct ttgaaatcgg ctcaggaaaa ggccatttta cccttgaatt 5940 agtaaagagg tgtaatttcg taactgccat tgaaatagac cataaattat gcaaaactac 6000 agaaaataaa cttgttgatc acgataattt ccaagtttta aacaaggata tattgcagtt 6060 taaatttcct aaaaaccaat cctataaaat atatggtaat ataccttata acataagtac 6120 ggatataata cgcaaaattg ttcttgatag tatagctaat gagatttatt taatcgtgga 6180 atacgggttt gctaaaagat tattaaatac aaaacgctca ttggcattac ttttaatggc 6240 agaagttgat atttctatat taagtatggt tccaagagaa tattttcatc ctaaacctaa 6300 agtgaatagc tcacttatca gattaagtag aaaaaaatca agaatatcac acaaagataa 6360 acaaaagtat aattatttcg ttctgaaatg ggttaacaaa gaatacaaga aaatatttac 6420 aaaaaatcaa tttaacaatt ccttaaaaca tgcaggaatt gacgatttaa acaatattag 6480 ctttgaacaa ttcttatctc ttgtcaatag ctataaatta tttaataagt aagttaaggg 6540 atgcataaac tgcatccctt aacttgtttt tcgtgtgcct attttttgtg aatcgtcgac 6600 aagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc accgtgtatg 6660 aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata 6720 ggcttggtta tgccggtact gccgggcctc ttgcgggatc gatagaaagc gtgagaaaca 6780 gcgtacagac gatttagaga tgtagaggta cttttatgcc gagaaaactt tttgcgtgtg 6840 acagtcctta aaatatactt agagcgtaag cgaaagtagt agcgacagct attaactttc 6900 ggttgcaaag ctctaggatt tttaatggac gcagcgcatc acacgcaaaa aggaaattgg 6960 aataaatgcg aaatttgaga tgttaattaa agaccttttt gaggtctttt tttcttagat 7020 ttttggggtt atttagggga gaaaacatag gggggtacta cgacctcccc cctaggtgtc 7080 cattgtccat tgtccaaaca aataaataaa tattgggttt ttaatgttaa aaggttgttt 7140 tttatgttaa agtgaaaaaa acagatgttg ggaggtacag tgatagttgt agatagaaaa 7200 gaagagaaaa aagttgctgt tactttaaga cttacaacag aagaaaatga gatattaaat 7260 agaatcaaag aaaaatataa tattagcaaa tcagatgcaa ccggtattct aataaaaaaa 7320 tatgcaaagg aggaatacgg tgcattttaa acaaaaaaag atagacagca ctggcatgct 7380 gcctatctat gactaaattt tgttaagtgt attagcaccg ttattatatc atgagcgaaa 7440 atgtaataaa agaaactgaa aacaagaaaa attcaagagg acgtaattgg acatttgttt 7500 tatatccaga atcagcaaag ccgagtggtt agagtattta aaagagttac acattcaatt 7560 tgtagtgctc cattacatga tagggatact gatacagaag gtaggatgaa aaaagagcat 7620 tatcatattc tagtgatgta tgagggtaat aaatcttatg aacagataaa aataataaca 7680 gaagaattga atgcgactat tccgcagatt gcaggaagtg tgaaaggtct tgtgagatat 7740 atgcttcaca tggacgatcc taataaattt aaatatcaaa aagaagatat gatagtttat 7800 ggcggtgtag atgttgatga attattaaag aaaacaacaa cagatagata taaattaatt 7860 aaagaaatga ttgagtttat tgatgaacaa ggaatcgtag aatttaagag tttaatggat 7920 tatgcaatga agtttaaatt tgatgattgg ttcccgcttt tatgtgataa ctcggcgtat 7980 gttattcaag aatatataaa atcaaatcgg tataaatctg accgatagat tttgaattta 8040 ggtgtcacaa gacactcttt tttcgcacca gcgaaaactg gtttaagccg agtgcgcaaa 8100 agacataatc gggaattc 8118 // ID MN913428; SV 1; circular; other DNA; STD; SYN; 7928 BP. XX AC MN913428; XX DT 21-JUN-2020 (Rel. 144, Created) DT 21-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning vector pRGPDuo2, complete sequence. XX KW . XX OS Cloning vector pRGPDuo2 OC other sequences; artificial sequences; vectors. XX RN [1] RC Publication Status: Available-Online prior to print RP 1-7928 RX PUBMED; 32504628. RA Gauttam R., Mukhopadhyay A., Singer S.W.; RT "Construction of a novel dual-inducible duet-expression system for gene RT (over)expression in Pseudomonas putida"; RL Plasmid 102514:0(2020). XX RN [2] RP 1-7928 RA Gauttam R., Singer S.W.; RT ; RL Submitted (08-JAN-2020) to the INSDC. RL Joint BioEnergy Institute, Lawrence Berkeley National Laboratory, 5885 RL Hollis Street, Emeryville, CA 94608, USA XX DR MD5; d5e4666222a873f87e1f1314959b3fab. XX CC ##Assembly-Data-START## CC Sequencing Technology :: Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..7928 FT /organism="Cloning vector pRGPDuo2" FT /mol_type="other DNA" FT /db_xref="taxon:2744475" FT rep_origin 1325..1913 FT /note="replication origin from E. coli ColE1" FT gene 2146..2961 FT /gene="aph" FT /note="confers kanamycin resistance" FT CDS 2146..2961 FT /codon_start=1 FT /transl_table=11 FT /gene="aph" FT /product="Aph" FT /note="confers kanamycin resistance" FT /protein_id="QKS02554.1" FT /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG FT KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT regulatory 3898..3951 FT /note="tetR/tetA promoter; overlapping promoters for FT bacterial tetR and tetA" FT /regulatory_class="promoter" FT gene 3971..4594 FT /gene="tetR" FT /note="from transposon Tn10" FT CDS 3971..4594 FT /codon_start=1 FT /transl_table=11 FT /gene="tetR" FT /product="TetR" FT /note="from transposon Tn10" FT /protein_id="QKS02556.1" FT /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV FT KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR FT PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT FT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS" FT rep_origin 5144..5977 FT /note="Replication origin from Pseudomonas putida" FT gene complement(6422..7396) FT /gene="lacI" FT /note="encodes Lac repressor" FT CDS complement(6422..7396) FT /codon_start=1 FT /transl_table=11 FT /gene="lacI" FT /product="LacI" FT /note="encodes Lac repressor" FT /protein_id="QKS02555.1" FT /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSR FT ADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPA FT LFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKY FT LTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLR FT VGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVS FT LVKRKTTLAPNTQTASPRASAAMPAIMACFSPKRLVAGPVTKA" FT regulatory 7831..7859 FT /note="tac promoter" FT /regulatory_class="promoter" XX SQ Sequence 7928 BP; 1938 A; 1980 C; 2077 G; 1933 T; 0 other; aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct cgaattcact 60 ggccgtcgtt ttacagccaa gcttggctgt tttggcggat gagagaagat tttcagcctg 120 atacagatta aatcagaacg cagaagcggt ctgataaaac agaatttgcc tggcggcagt 180 agcgcggtgg tcccacctga ccccatgccg aactcagaag tgaaacgccg tagcgccgat 240 ggtagtgtgg ggtctcccca tgcgagagta gggaactgcc aggcatcaaa taaaacgaaa 300 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcct 360 gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc ccggagggtg 420 gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg ccatcctgac 480 ggatggcctt tttgcgtttc tacaaactct tttgtttatt tttctaaata cattcaaata 540 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 600 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 660 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 720 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 780 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 840 cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 900 tggttgagta attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca 960 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 1020 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 1080 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 1140 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 1200 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 1260 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 1320 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 1380 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 1440 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 1500 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 1560 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 1620 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 1680 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 1740 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag 1800 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 1860 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 1920 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 1980 tggtcatgag attatcaaaa aggatcttca cctagatcct tttggggggg gggggaaagc 2040 cacgttgtgt ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga 2100 acaataaaac tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa 2160 cgggaaacgt cttgctcgag gccgcgatta aattccaaca tggatgctga tttatatggg 2220 tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg attgtatggg 2280 aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt 2340 acagatgaga tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag 2400 cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca 2460 gcattccagg tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca 2520 gtgttcctgc gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc 2580 gtatttcgtc tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat 2640 tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataagctt 2700 ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt 2760 tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga 2820 taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa 2880 cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg 2940 atgctcgatg agtttttcta atcagaattg gttaattggt tgtaacactg gcagagcatt 3000 acgctgactt gacgggacgg cggctttgtt gaataaatcg aacttttgct gagttgaagg 3060 atcagatcac gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa 3120 tcaccaactg gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc 3180 tggatgatgg ggcgattcag gcctggtatg agtcagcaac accttcttca cgaggcagac 3240 ctcagcgccc ccccccccct agcttgtcta cgtctgatgc tttgaatcgg acggacttgc 3300 cgatcttgta tgcggtgatt tttccctcgt ttgcccactt tttaatggtg gccggggtga 3360 gagctacgcg ggcggcgacc tgctgcgctg tgatccaata ttcggggtcg ttcactggtt 3420 cccctttctg atttctggca tagaagaacc cccgtgaact gtgtggttcc gggggttgct 3480 gatttttgcg agacttctcg cgcaattccc tagcttaggt gaaaacacca tgaaacacta 3540 gggaaacacc catgaaacac ccattagggc agtagggcgg cttcttcgtc tagggcttgc 3600 atttgggcgg tgatctggtc tttagcgtgt gaaagtgtgt cgtaggtggc gtgctcaatg 3660 cactcgaacg tcacgtcatt taccgggtca cggtgggcaa agagaactag tttcgaaacg 3720 cagaaaggcc cacccgaagg tgagccaata aaacgaaagg cccagtcttt cgactgagcc 3780 tttcgtttta tttgatgcct ggaccgcggt actccgcggg ctgaggacat gttgtacaat 3840 ttaaatccta gggctagcgt ataccctttc ctcctcttta gatcttttga attcttttct 3900 ctatcactga tagggagtgg taaaataact ctatcaacga tagagtgtca acaaaaatta 3960 ggaattaatg atgtctagat tagataaaag taaagtgatt aacagcgcat tagagctgct 4020 taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc taggtgtaga 4080 gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg ccttagccat 4140 tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa gctggcaaga 4200 ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc gcgatggagc 4260 aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg aaaatcaatt 4320 agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac tcagcgctgt 4380 ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg ctaaagaaga 4440 aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta tcgaattatt 4500 tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca tatgcggatt 4560 agaaaaacaa cttaaatgtg aaagtgggtc ttaagacgtc gatcaattga gccatgaaaa 4620 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc 4680 gcatacgcta cttgcattac agtttacgaa ccgaacaggc ttatgtcaat tcgcctctca 4740 ggcgccgctg gtgccgctgg ttggacgcca agggtgaatc cgcctcgata ccctgattac 4800 tcgcttcctg cgccctctca ggcggcgata ggggactggt aaaacgggga ttgcccagac 4860 gcctcccccg ccccttcagg ggcacaaatg cggccccaac ggggccacgt agtggtgcgt 4920 tttttgcgtt tccacccttt tcttcctttt cccttttaaa ccttttagga cgtctacagg 4980 ccacgtaatc cgtggcctgt agagtttaaa aagggacgga tttgttgcca ttaagggacg 5040 gatttgttgt taagaaggga cggatttgtt gttgtaaagg gacggatttg ttgtattgtg 5100 ggacgcagat acagtgtccc cttatacaca aggaatgtcg aacgtggcct cacccccaat 5160 ggtttacaaa agcaatgccc tggtcgaggc cgcgtatcgc ctcagtgttc aggaacagcg 5220 gatcgttctg gcctgtatta gccaggtgaa gaggagcgag cctgtcaccg atgaagtgat 5280 gtattcagtg acggcggagg acatagcgac gatggcgggt gtccctatcg aatcttccta 5340 caaccagctc aaagaagcgg ccctgcgcct gaaacggcgg gaagtccggt taacccaaga 5400 gcccaatggc aaggggaaaa gaccgagtgt gatgattacc ggctgggtgc aaacaatcat 5460 ctaccgggag ggtgagggcc gtgtagaact caggttcacc aaagacatgc tgccgtacct 5520 gacggaactc accaaacagt tcaccaaata cgccttggct gacgtggcca agatggacag 5580 cacccacgcg atcaggcttt acgagctgct catgcaatgg gacagcatcg gccagcgcga 5640 aatagaaatt gaccagctgc gaaagtggtt tcaactggaa ggccggtatc cctcgatcaa 5700 ggacttcaag ttgcgagtgc ttgatccagc cgtgacgcag atcaacgagc acagcccgct 5760 acaggtggag tgggcgcagc gaaagaccgg gcgcaaggtc acacatctgt tgttcagttt 5820 tggaccgaag aagcccgcca aggcggtggg taaggcccca gcgaagcgca aggccgggaa 5880 gatttcagat gctgagatcg cgaaacaggc tcgccctggt gagacatggg aagcggcccg 5940 cgctcgacta acccagatgc cgctggatct ggcctagagg ccgtggccac cacggcccgg 6000 cctgcctttc aggctgcgca actgcgtacg aggcccggcg ccagcgcgag gcgatcgtgt 6060 ccgccgtggc gtcggcagtc gctcgccgag atacgtcacg tgacgaaatg cagcagcctt 6120 ccattccgtc acgtgacgaa actcgggccg caggtcagag cacggttccg cccgctccgg 6180 ccctgccgga cccccggcat cccgcaagag gcccggcagt accggcataa ccaagcctat 6240 gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat 6300 acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc 6360 gatgataagc tgtcaaacat ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg 6420 ctcaagcctt cgtcactggt cccgccacca aacgtttcgg cgagaagcag gccattatcg 6480 ccggcatggc ggccgacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt 6540 ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga 6600 gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg 6660 ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatat 6720 ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat 6780 cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt 6840 gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc 6900 gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc 6960 ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg 7020 taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa 7080 ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg 7140 gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac 7200 aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg 7260 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg 7320 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt 7380 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg 7440 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt 7500 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg 7560 ccataccgcg aaaggttttg caccattcga tggtgtcaac gtaaatgcat gccgcttcgc 7620 cttcgcgcgc gaattgcaag ctgatccggg cttatcgact gcacggtgca ccaatgcttc 7680 tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa tcactgcata 7740 attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa 7800 cggttctggc aaatattctg aaatgagctg ttgacaatta atcatcggct cgtataatgt 7860 gtggaattgt gagcggataa caatttcaca caggaaacag aattaaaaga tatgaccatg 7920 attacgcc 7928 // ID MT453110; SV 1; circular; other DNA; STD; SYN; 10220 BP. XX AC MT453110; XX DT 21-JUN-2020 (Rel. 144, Created) DT 21-JUN-2020 (Rel. 144, Last updated, Version 1) XX DE Cloning vector pBXL_attP_PUbDsRed.T3_fa_s1-tTA_a, complete sequence. XX KW . XX OS Cloning vector pBXL_attP_PUbDsRed.T3_fa_s1-tTA_a OC other sequences; artificial sequences; vectors. XX RN [1] RP 1-10220 RA Zhao Y., Handler A.M., Schetelig M.F.; RT "Genetic breakdown of a Tet-off lethality system for insect population RT control"; RL Unpublished. XX RN [2] RP 1-10220 RA Schetelig M.F.; RT ; RL Submitted (07-MAY-2020) to the INSDC. RL Department of Insect Biotechnology in Plant Protection, RL Justus-Liebig-University Giessen, Institute for Insect Biotechnology, RL Winchesterstr. 2, Giessen, Hesse 35394, Germany XX DR MD5; f1bf1d6f12882868601c639d923013df. XX FH Key Location/Qualifiers FH FT source 1..10220 FT /organism="Cloning vector FT pBXL_attP_PUbDsRed.T3_fa_s1-tTA_a" FT /mol_type="other DNA" FT /db_xref="taxon:2744358" FT mobile_element join(83..391,3777..3977) FT /mobile_element_type="transposon:piggyBac" FT misc_recomb 4035..4268 FT /note="attP" FT regulatory 4322..6319 FT /note="PUb" FT /regulatory_class="promoter" FT gene 6341..7018 FT /gene="DsRed.T3" FT CDS 6341..7018 FT /codon_start=1 FT /transl_table=11 FT /gene="DsRed.T3" FT /product="DsRed.T3" FT /protein_id="QKQ15145.1" FT /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK FT LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV FT VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK FT ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH FT LFL" FT regulatory 7019..7257 FT /note="SV40" FT /regulatory_class="polyA_signal_sequence" FT regulatory 7367..7677 FT /note="serendipity alpha (s1)" FT /regulatory_class="promoter" FT gene 7685..8692 FT /gene="tTA" FT CDS 7685..8692 FT /codon_start=1 FT /transl_table=11 FT /gene="tTA" FT /product="tTA" FT /protein_id="QKQ15146.1" FT /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV FT KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR FT PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT FT DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI FT EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA FT DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG" FT regulatory 8709..10106 FT /note="K10" FT /regulatory_class="polyA_signal_sequence" XX SQ Sequence 10220 BP; 2764 A; 2411 C; 2449 G; 2596 T; 0 other; cgcgcctaga tcttaatacg actcactata gggcgaattg ggtaccgggc cccccctcga 60 ggtcgacggt atcgataagc ttgatatcta taacaagaaa atatatatat aataagttat 120 cacgtaagta gaacatgaaa taacaatata attatcgtat gagttaaatc ttaaaagtca 180 cgtaaaagat aatcatgcgt cattttgact cacgcggtcg ttatagttca aaatcagtga 240 cacttaccgc attgacaagc acgcctcacg ggagctccaa gcggcgactg agatgtccta 300 aatgcacagc gacggattcg cgctatttag aaagagagag caatatttca agaatgcatg 360 cgtcaatttt acgcagacta tctttctagg gttaatctag ctgcatcagg atcatatcgt 420 cgggtctttt ttccggctca gtcatcgccc aagctggcgc tatctgggca tcggggagga 480 agaagcccgt gccttttccc gcgaggttga agcggcatgg aaagagtttg ccgaggatga 540 ctgctgctgc attgacgttg agcgaaaacg cacgtttacc atgatgattc gggaaggtgt 600 ggccatgcac gcctttaacg gtgaactgtt cgttcaggcc acctgggata ccagttcgtc 660 gcggcttttc cggacacagt tccggatggt cagcccgaag cgcatcagca acccgaacaa 720 taccggcgac agccggaact gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct 780 gggatattac gtcagcgagg acgggtatcc tggctggatg ccgcagaaat ggacatggat 840 accccgtgag ttacccggcg ggcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg 900 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 960 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 1020 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 1080 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 1140 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 1200 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 1260 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 1320 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 1380 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 1440 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 1500 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 1560 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 1620 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 1680 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 1740 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 1800 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 1860 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 1920 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 1980 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 2040 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 2100 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 2160 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 2220 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 2280 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 2340 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 2400 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 2460 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 2520 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 2580 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 2640 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 2700 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 2760 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 2820 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 2880 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 2940 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 3000 gttccgcgca catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa 3060 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 3120 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 3180 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 3240 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 3300 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 3360 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 3420 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 3480 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg 3540 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg 3600 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag cgcgcctcgt 3660 tcattcacgt ttttgaaccc gtggaggacg ggcagactcg cggtgcaaat gtgttttaca 3720 gcgtgatgga gcagatgaag atgctcgaca cgctgcagaa cacgcagcta gattaaccct 3780 agaaagataa tcatattgtg acgtacgtta aagataatca tgcgtaaaat tgacgcatgt 3840 gttttatcgg tctgtatatc gaggtttatt tattaatttg aatagatatt aagttttatt 3900 atatttacac ttacatacta ataataaatt caacaaacaa tttatttatg tttatttatt 3960 tattaaaaaa aaacaaaaac tcaaaatttc ttctataaag taacaaaact tttatcgaat 4020 tcctgcagcc cgggactagt actgacggac acaccgaagc cccggcggca accctcagcg 4080 gatgccccgg ggcttcacgt tttcccaggt cagaagcggt tttcgggagt agtgccccaa 4140 ctggggtaac ctttgagttc tctcagttgg gggcgtaggg tcgccgacat gacacaaggg 4200 gttgtgaccg gggtggacac gtacgcgggt gcttacgacc gtcagtcgcg cgagcgcgac 4260 tagtacagcc cgggggatct cgagctcaag cttcgaattc tgcagtcgac ggtacccgat 4320 cttgtcgccg gaacgcagcg acagagattc caatgtgtcc gtatctttca ggcttttgcc 4380 cttcagttcc agac