ID MN020371; SV 1; linear; genomic DNA; STD; TGN; 7729 BP. XX AC MN020371; XX DT 20-JUL-2020 (Rel. 144, Created) DT 20-JUL-2020 (Rel. 144, Last updated, Version 1) XX DE Triticum aestivum transgenic MON71200 insertion site, genomic sequence. XX KW . XX OS Triticum aestivum (bread wheat) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade; OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum. XX OS synthetic construct OC other sequences; artificial sequences. XX RN [1] RP 1-7729 RA Duceppe M.-O., Koziol A.G., Colville A., Dupras A.A., Ogunremi D., RA Cote M.-J., Gagnon M.-C.; RT ; RL Submitted (03-JUN-2019) to the INSDC. RL Canadian Food Inspection Agency, Gouvernment of Canada, 3851, Fallowfield RL Road, Ottawa, ON K2H 8P9, Canada XX DR MD5; b70e6ad64a4cf990fda9b5dbdb2f8a00. XX CC ##Assembly-Data-START## CC Assembly Method :: Canu v. 1.7; SPAdes v. 3.11.1 CC Assembly Name :: MON71200_v1 CC Coverage :: 50X (approx) CC Sequencing Technology :: Illumina; Oxford Nanopore Technologies CC MinION; Sanger dideoxy sequencing CC ##Assembly-Data-END## XX FH Key Location/Qualifiers FH FT source 1..7729 FT /organism="Triticum aestivum" FT /transgenic FT /mol_type="genomic DNA" FT /db_xref="taxon:4565" FT source 37..6147 FT /organism="synthetic construct" FT /mol_type="genomic DNA" FT /clone="MON71200_v1" FT /db_xref="taxon:32630" FT misc_feature 1..7729 FT /note="insertion site of Monsanto event MON71200" FT misc_feature 1..36 FT /note="5' wheat flanking sequence" FT regulatory complement(37..235) FT /note="dervied from Oryza sativa actin 1 promoter fragment" FT /regulatory_class="promoter" FT regulatory 237..325 FT /note="35S promoter fragment" FT /regulatory_class="promoter" FT intron 354..803 FT /note="derived from Oryza sativa actin 1" FT CDS 804..2399 FT /codon_start=1 FT /product="CTP2-CP4-EPSPS" FT /note="derived from Arabidopsis thaliana and Agrobacterium FT tumefaciens" FT /protein_id="QLF98898.1" FT /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI FT SSSWGLKKSGMTLIGSELRPLKVMSSVSTACMLHGASSRPATARKSSGLSGTVRIPGDK FT SISHRSFMFGGLASGETRITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGL FT LAPEAPLDFGNAATGCRLTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKS FT EDGDRLPVTLRGPKTPTPITYRVPMASAQVKSAVLLAGLNTPGITTVIEPIMTRDHTEK FT MLQGFGANLTVETDADGVRTIRLEGRGKLTGQVIDVPGDPSSTAFPLVAALLVPGSDVT FT ILNVLMNPTRTGLILTLQEMGADIEVINPRLAGGEDVADLRVRSSTLKGVTVPEDRAPS FT MIDEYPILAVAAAFAEGATVMNGLEELRVKESDRLSAVANGLKLNGVDCDEGETSLVVR FT GRPDGKGLGNASGAAVATHLDHRIAMSFLVMGLVSENPVTVDDATMIATSFPEFMDLMA FT GLGAKIELSDTKAA" FT regulatory 2400..2667 FT /note="NOS terminator; derived from Agrobacterium FT tumefaciens" FT /regulatory_class="terminator" FT regulatory 2684..3296 FT /note="35S promoter; derived from cauliflower mosaic virus" FT /regulatory_class="promoter" FT intron 3325..3774 FT /note="derived from Oryza sativa actin 1 intron" FT CDS 3775..5370 FT /codon_start=1 FT /product="CTP2-CP4-EPSPS" FT /note="derived from Arabidopsis thaliana and Agrobacterium FT tumefaciens" FT /protein_id="QLF98899.1" FT /translation="MAQVSRICNGVQNPSLISNLSKSSQRKSPLSVSLKTQQHPRAYPI FT SSSWGLKKSGMTLIGSELRPLKVMSSVSTACMLHGASSRPATARKSSGLSGTVRIPGDK FT SISHRSFMFGGLASGETRITGLLEGEDVINTGKAMQAMGARIRKEGDTWIIDGVGNGGL FT LAPEAPLDFGNAATGCRLTMGLVGVYDFDSTFIGDASLTKRPMGRVLNPLREMGVQVKS FT EDGDRLPVTLRGPKTPTPITYRVPMASAQVKSAVLLAGLNTPGITTVIEPIMTRDHTEK FT MLQGFGANLTVETDADGVRTIRLEGRGKLTGQVIDVPGDPSSTAFPLVAALLVPGSDVT FT ILNVLMNPTRTGLILTLQEMGADIEVINPRLAGGEDVADLRVRSSTLKGVTVPEDRAPS FT MIDEYPILAVAAAFAEGATVMNGLEELRVKESDRLSAVANGLKLNGVDCDEGETSLVVR FT GRPDGKGLGNASGAAVATHLDHRIAMSFLVMGLVSENPVTVDDATMIATSFPEFMDLMA FT GLGAKIELSDTKAA" FT regulatory 5371..5638 FT /note="NOS terminator; derived from Agrobacterium FT tumefaciens" FT /regulatory_class="terminator" FT intron 5670..5872 FT /note="derived from Oryza sativa actin 1 intron fragment" FT misc_feature 5882..6142 FT /note="CTP2-CP4-EPSPS fragment; derived from Agrobacterium FT tumefaciens" FT misc_feature 6148..7729 FT /note="3' wheat flanking sequence" XX SQ Sequence 7729 BP; 1660 A; 2095 C; 2087 G; 1887 T; 0 other; gttacgtatt gctgaacacg cgtcattacc tccggacacc tgctgttttt tctttttctt 60 ttttttcttt cttttttttt ttggctgcga gacgtgcggt gcgtgcggac aactcacggt 120 gatagtgggg gggtgtggag actattgtcc agttggctgg actggggtgg gttgggttgg 180 gttgggttgg gctgggcttg ctatggatcg tggatagcac tttgggcttt aggaaactcc 240 actgacgtaa gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa 300 ggaagttcat ttcatttgga gaggacacgc tgacaagctg actctagcag atccctccgc 360 cgccgccggt aaccaccccg cccctctcct ctttctttct ccgttttttt ttccgtctcg 420 gtctcgatct ttggccttgg tagtttgggt gggcgagagg cggcttcgtg cgcgcccaga 480 tcggtgcgcg ggaggggcgg gatctcgcgg ggaatggggc tctcggatgt agatctgcga 540 tccgccgttg ttgggggaga tgatgggggg tttaaaattt ccgccgtgct aaacaagatc 600 aggaagaggg gaaaagggca ctatggttta tatttttata tatttctgct gcttcgtcag 660 gcttagatgt gctagatctt tctttcttct ttttgtgggt agaatttaat ccctcagcat 720 tgttcatcgg tagtttttct tttcatgatt tgtgacaaat gcagcctcgt gcggagcttt 780 tttgtaggta gaagtgatca accatggcgc aagttagcag aatctgcaat ggtgtgcaga 840 acccatctct tatctccaat ctctcgaaat ccagtcaacg caaatctccc ttatcggttt 900 ctctgaagac gcagcagcat ccacgagctt atccgatttc gtcgtcgtgg ggattgaaga 960 agagtgggat gacgttaatt ggctctgagc ttcgtcctct taaggtcatg tcttctgttt 1020 ccacggcgtg catgcttcac ggtgcaagca gccggcccgc aaccgcccgc aaatcctctg 1080 gcctttccgg aaccgtccgc attcccggcg acaagtcgat ctcccaccgg tccttcatgt 1140 tcggcggtct cgcgagcggt gaaacgcgca tcaccggcct tctggaaggc gaggacgtca 1200 tcaatacggg caaggccatg caggcgatgg gcgcccgcat ccgtaaggaa ggcgacacct 1260 ggatcatcga tggcgtcggc aatggcggcc tcctggcgcc tgaggcgccg ctcgatttcg 1320 gcaatgccgc cacgggctgc cgcctgacga tgggcctcgt cggggtctac gatttcgaca 1380 gcaccttcat cggcgacgcc tcgctcacaa agcgcccgat gggccgcgtg ttgaacccgc 1440 tgcgcgaaat gggcgtgcag gtgaaatcgg aagacggtga ccgtcttccc gttaccttgc 1500 gcgggccgaa gacgccgacg ccgatcacct accgcgtgcc gatggcctcc gcacaggtga 1560 agtccgccgt gctgctcgcc ggcctcaaca cgcccggcat cacgacggtc atcgagccga 1620 tcatgacgcg cgatcatacg gaaaagatgc tgcagggctt tggcgccaac cttaccgtcg 1680 agacggatgc ggacggcgtg cgcaccatcc gcctggaagg ccgcggcaag ctcaccggcc 1740 aagtcatcga cgtgccgggc gacccgtcct cgacggcctt cccgctggtt gcggccctgc 1800 ttgttccggg ctccgacgtc accatcctca acgtgctgat gaaccccacc cgcaccggcc 1860 tcatcctgac gctgcaggaa atgggcgccg acatcgaagt catcaacccg cgccttgccg 1920 gcggcgaaga cgtggcggac ctgcgcgttc gctcctccac gctgaagggc gtcacggtgc 1980 cggaagaccg cgcgccttcg atgatcgacg aatatccgat tctcgctgtc gccgccgcct 2040 tcgcggaagg ggcgaccgtg atgaacggtc tggaagaact ccgcgtcaag gaaagcgacc 2100 gcctctcggc cgtcgccaat ggcctcaagc tcaatggcgt ggattgcgat gagggcgaga 2160 cgtcgctcgt cgtgcgtggc cgccctgacg gcaaggggct cggcaacgcc tcgggcgccg 2220 ccgtcgccac ccatctcgat caccgcatcg ccatgagctt cctcgtcatg ggcctcgtgt 2280 cggaaaaccc tgtcacggtg gacgatgcca cgatgatcgc cacgagcttc ccggagttca 2340 tggacctgat ggccgggctg ggcgcgaaga tcgaactctc cgatacgaag gctgcctgat 2400 gagctcgaat tcccgatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg 2460 ttgccggtct tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa 2520 ttaacatgta atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat 2580 tatacattta atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc 2640 gcgcggtgtc atctatgtta ctagatcggg gatagcttct gcaggtccga ttgagacttt 2700 tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta tctgtcactt 2760 tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg 2820 aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac ccccacccac 2880 gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag tggattgatg 2940 tgatggtccg attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca 3000 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa 3060 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc 3120 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc 3180 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca 3240 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggacacg 3300 ctgacaagct gactctagca gatccctccg ccgccgccgg taaccacccc gcccctctcc 3360 tctttctttc tccgtttttt tttccgtctc ggtctcgatc tttggccttg gtagtttggg 3420 tgggcgagag gcggcttcgt gcgcgcccag atcggtgcgc gggaggggcg ggatctcgcg 3480 gggaatgggg ctctcggatg tagatctgcg atccgccgtt gttgggggag atgatggggg 3540 gtttaaaatt tccgccgtgc taaacaagat caggaagagg ggaaaagggc actatggttt 3600 atatttttat atatttctgc tgcttcgtca ggcttagatg tgctagatct ttctttcttc 3660 tttttgtggg tagaatttaa tccctcagca ttgttcatcg gtagtttttc ttttcatgat 3720 ttgtgacaaa tgcagcctcg tgcggagctt ttttgtaggt agaagtgatc aaccatggcg 3780 caagttagca gaatctgcaa tggtgtgcag aacccatctc ttatctccaa tctctcgaaa 3840 tccagtcaac gcaaatctcc cttatcggtt tctctgaaga cgcagcagca tccacgagct 3900 tatccgattt cgtcgtcgtg gggattgaag aagagtggga tgacgttaat tggctctgag 3960 cttcgtcctc ttaaggtcat gtcttctgtt tccacggcgt gcatgcttca cggtgcaagc 4020 agccggcccg caaccgcccg caaatcctct ggcctttccg gaaccgtccg cattcccggc 4080 gacaagtcga tctcccaccg gtccttcatg ttcggcggtc tcgcgagcgg tgaaacgcgc 4140 atcaccggcc ttctggaagg cgaggacgtc atcaatacgg gcaaggccat gcaggcgatg 4200 ggcgcccgca tccgtaagga aggcgacacc tggatcatcg atggcgtcgg caatggcggc 4260 ctcctggcgc ctgaggcgcc gctcgatttc ggcaatgccg ccacgggctg ccgcctgacg 4320 atgggcctcg tcggggtcta cgatttcgac agcaccttca tcggcgacgc ctcgctcaca 4380 aagcgcccga tgggccgcgt gttgaacccg ctgcgcgaaa tgggcgtgca ggtgaaatcg 4440 gaagacggtg accgtcttcc cgttaccttg cgcgggccga agacgccgac gccgatcacc 4500 taccgcgtgc cgatggcctc cgcacaggtg aagtccgccg tgctgctcgc cggcctcaac 4560 acgcccggca tcacgacggt catcgagccg atcatgacgc gcgatcatac ggaaaagatg 4620 ctgcagggct ttggcgccaa ccttaccgtc gagacggatg cggacggcgt gcgcaccatc 4680 cgcctggaag gccgcggcaa gctcaccggc caagtcatcg acgtgccggg cgacccgtcc 4740 tcgacggcct tcccgctggt tgcggccctg cttgttccgg gctccgacgt caccatcctc 4800 aacgtgctga tgaaccccac ccgcaccggc ctcatcctga cgctgcagga aatgggcgcc 4860 gacatcgaag tcatcaaccc gcgccttgcc ggcggcgaag acgtggcgga cctgcgcgtt 4920 cgctcctcca cgctgaaggg cgtcacggtg ccggaagacc gcgcgccttc gatgatcgac 4980 gaatatccga ttctcgctgt cgccgccgcc ttcgcggaag gggcgaccgt gatgaacggt 5040 ctggaagaac tccgcgtcaa ggaaagcgac cgcctctcgg ccgtcgccaa tggcctcaag 5100 ctcaatggcg tggattgcga tgagggcgag acgtcgctcg tcgtgcgtgg ccgccctgac 5160 ggcaaggggc tcggcaacgc ctcgggcgcc gccgtcgcca cccatctcga tcaccgcatc 5220 gccatgagct tcctcgtcat gggcctcgtg tcggaaaacc ctgtcacggt ggacgatgcc 5280 acgatgatcg ccacgagctt cccggagttc atggacctga tggccgggct gggcgcgaag 5340 atcgaactct ccgatacgaa ggctgcctga tgagctcgaa ttcccgatcg ttcaaacatt 5400 tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa 5460 tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg 5520 agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa 5580 atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg 5640 ggatatcccc ggggaattcg gtaccaagta acaagatcag gaagagggga aaagggcact 5700 atggtttata tttttatata tttctgctgc ttcgtcaggc ttagatgtgc tagatctttc 5760 tttcttcttt ttgtgggtag aatttaatcc ctcagcattg ttcatcggta gtttttcttt 5820 tcatgatttg tgacaaatgc agcctcgtgc ggagcttttt tgtaggtaga agtgatcaac 5880 catggcgcaa gttagcagaa tctgcaatgg tgtgcagaac ccatctctta tctccaatct 5940 ctcgaaatcc agtcaacgca aatctccctt atcggtttct ctgaagacgc agcagcatcc 6000 acgagccgat cacctaccgc gtgccgatgg cctccgcaca ggtgaagtcc gccgtgctgc 6060 tcgccggcct caacacgccc ggcatcacga cggtcatcga gccgatcatg acgcgcgatc 6120 atacggaaaa gatgctgcag ggaatatatt gaactagtta gtttaattaa atagtaatta 6180 gattagttaa accctaatta gactaaacag tcaatgacga acgggaccca catgtcaggt 6240 tgactaggcc aactgctgac gtcatgatga cgtcagcaaa tactgttttg gataatgttg 6300 attttaataa ttaaataaat cctaaaattg atttaaatct tttaagatta atatagaata 6360 aaccgtagct cggatggaaa aactttgtac atgaaatttg ctcaaaacga cgagtcgaat 6420 tcggatacgc agcccgttcg tccgccacgc atccctagca tagtgaacac gcaactttcc 6480 cctccgattc atctgtccga aaacgcaaaa cactggggat attttcccgg atgtttcctc 6540 ccttcaccgg tatcacctca taccacgtta gggcacccta gcaccgctac ttgccatgtc 6600 atgcatcgcc atgcatttgt ttgcattata tttattgttt ctcccctctt ctctcgctag 6660 actccgagac cgacgccgct gctacccagt acgactacgg tgttgacgac ccttccttct 6720 tgtcagagca accaggcaag cccccttgat caccagatat ggcctactct cctgtatact 6780 gcttgcatta gagtagtgtg gcatgttact gctttccgtt aatcttattc tgttgcatag 6840 cctgtcattg ttgctacaac tgttgatacc ttacctgcaa tcctaaatgc ttagtatagg 6900 atgctaatgt tccatcagtg gccctacact cttgtccgtc tgccatgcta tactactggg 6960 ccgtgatcac tcgggaggtg atcacgggca catatatact tatactgtta cattacttat 7020 gatactgttc ggagatgggg gctgaagggg ccggtggctc catccaggta gtggtgggcc 7080 tgagttcccg atggccccga ctgttacttt gaggtggagc gacaaggcag gttgagacca 7140 cctaggagag aggtgggctt ggccctggtc ggcgttcgcg gatacttaac acgtttaacg 7200 agatcttggt atttgatctg agtctggcta ctggccgata tgcactaacc atctacgcgg 7260 ggatagttat gggcactcga cgtcgtggta tcagccgaag cctttgtgac gtcagcgacc 7320 gagcggcgcg cgtcgggttg gacctcgtaa cacaacttcc tttgtaatgg aggttgctag 7380 gtctgctcac cggccgcgta cgcaacgtgc aggtgtgcaa tgggcgatgg gcctgaaccg 7440 ctgcgccgta ggatttagac cggcgtgctg acttctctgt tgtgcctagg tagggatgcg 7500 acgtgttgat cttccgaggc cgggcatgac cagaaaagtg tgtccggaca aagtggatcg 7560 agcatgttgg aaaatgtggt gcacctgcag tggttgatct attcgaatag ccgtgtccct 7620 cgataaagga cgacccggag ttgtaccttg accttatgac aactagaacc ggatacttaa 7680 taaaacacac ccttcaagtg cgagatacaa ccggtgatcg ctctctcac 7729 //